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From: PESTOEM-at-aol.com
Date: Sat, 1 Jan 2000 14:46:25 EST
Subject: Jeol 1200 Alignment

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Happy New Year to all and especially all who responded to my call for help.
Thanks again. Best wishes for a great year. Sincerely, Peter A.
Stolzenberg,Pesto Inc.



From daemon Sat Jan 01 16:49:40 2000



From: Tom Phillips :      PhillipsT-at-missouri.edu
Date: Sat, 1 Jan 2000 14:39:45 -0600
Subject: Re: Potential for expansion of microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


sounds like a research grant to me. I don't believe the salary of
faculty in Chemistry departments of most major colleges would be
"soft money" requiring grant support. I commend the Society of
Analytical Chemists for trying to encourage this bridge. 20K of seed
money for a new line of research can make an important difference to
a junior faculty member.


} ---------------------------------------------------------------.
}
}
} Hum....so for a 2000 hour year, this works out to be $10 per hour.
} What a gift. And they probably want $30/hour of work in return.
}
} No matter how you package it, all of this babble does not measure
} up to today's standards. Unless SEM, etc. is a obscure and
} diminutive endeavor, I simply do not understand the cost-benefit
} ratio. Maybe this is not an annual salary. OK. Is this in addition
} to an existing income stream? Geeze, I hope it is the latter. But it
} sounds like the position is on-site. So, the candidate gets a full time
} job at McDonald's as well?
}
} All I can say is that I am glad and relieved that I do not have to
} work and try to survive in this type of environment. Welcome to H-2 visas.
}
} gary g.
}
}
} At 08:14 AM 12/31/99 , you wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } I just received this notification from the Society for Analytical Chemists
} } of Pittsburgh. Perhaps it will provide an important start for a new chem
} } prof who has an interest in expanding the use of microscopy and/or in
} } walking across the new bridge between microscopy and spectroscopy:
} }
} } "Twenty-first annual Analytical Chemistry Starter Grant Award"
} } The society for Analytical Chemists of Pittsburgh will award one grant of
} } $20,000 to an assistant professor in the field of analytical chemistry.
} } The purpose of this grant is to encourage high-quality, innovative research
} } by a new analytical chemistry professor and to promote the training and
} } development of graduate students in this field. Assistant professors who
} } have accepted a US college or university appoint since December 31, 1996
} } are eligible. Application forms available from:
} } James Chadwick, Chairman
} } Starter Grant Committee
} } Society for Analytical Chemists of Pittsburgh
} } 200 Penn Center Blvd., Suite 332
} } Pittsburgh, PA 15235
} } Ph: 1-800-825-3221, Xt. 208
} } Fx: 412-825-3224
} }
} } Deadline for application receipt: February 29, 2000
} } Award winner announced: May 1, 2000
} }
} }
} } Best regards and welcome to the new millennium!
} } Barbara Foster
} } Consortium President
} } Microscopy/Microscopy Education ...Educating microscopists for greater
} } productivity.
} }
} } 125 Paridon Street Suite 102 Springfield, MA 01118
} } PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com
} } Visit our web site {http://www.MME-Microscopy.com/education}
} } ******************************************************
} } MME is America's first national consortium providing
} } customized on-site workshops in all areas of
} } microscopy, sample preparation, and image analysis.

Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)



From daemon Sat Jan 01 17:10:58 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Sat, 01 Jan 2000 14:43:01 -0800
Subject: Re: Potential for expansion of microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-----BEGIN PGP SIGNED MESSAGE-----
Hash: SHA1

Thanks for the info. I understand what is going on now. sounds like a great opportunity.

I discussed compensation before and don't want to clutter up the list with a repeat. But I
would appreciate an off-list communication which helps me better understand how the
mechanics of SEM and its personnel work in the big picture. I get asked often how to
get into the field. Since I use my SEM for personal research and photography only, I am
not in academia or companys' formal research departments. If I sound ignorant about
this, it is because I am. I work with some high school and upper level pre-high school
students at times using my SEM and LMs. What can be done with the SEM and LMs
turns them on as much as it still does me today. I'm hopelessly hooked.

But I lack facts and figures about this type of career. Rather than speculating about it,
what do you careerists think? Is this a good career? What is involved in getting into
the career at various levels? And what compensation is commensurate at these levels?

All responses will be kept confidential. Use PGP if you need to.

gary g.




At 12:39 PM 1/1/00 , you wrote:
} sounds like a research grant to me. I don't believe the salary of faculty in Chemistry departments of most major colleges would be "soft money" requiring grant support. I commend the Society of Analytical Chemists for trying to encourage this bridge. 20K of seed money for a new line of research can make an important difference to a junior faculty member.
}
}
} } ---------------------------------------------------------------.
} }
} }
} } Hum....so for a 2000 hour year, this works out to be $10 per hour.
} } What a gift. And they probably want $30/hour of work in return.
} }
} } No matter how you package it, all of this babble does not measure
} } up to today's standards. Unless SEM, etc. is a obscure and
} } diminutive endeavor, I simply do not understand the cost-benefit
} } ratio. Maybe this is not an annual salary. OK. Is this in addition
} } to an existing income stream? Geeze, I hope it is the latter. But it
} } sounds like the position is on-site. So, the candidate gets a full time
} } job at McDonald's as well?
} }
} } All I can say is that I am glad and relieved that I do not have to
} } work and try to survive in this type of environment. Welcome to H-2 visas.
} }
} } gary g.
} }
} }
} } At 08:14 AM 12/31/99 , you wrote:
} } } ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } -----------------------------------------------------------------------.
} } }
} } }
} } } I just received this notification from the Society for Analytical Chemists
} } } of Pittsburgh. Perhaps it will provide an important start for a new chem
} } } prof who has an interest in exd, MA 01118
PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com
Visit our web site {http://www.MME-Microscopy.com/education}
******************************************************
MME is America's first national consortium providing
customized on-site workshops in all areas of
microscopy, sample preparation, and image analysis.


--CAB25050.946689051/kepler.fmph.uniba.sk--


The subject of the message is: tacky wax

The address to which the message has not yet been delivered is:

cg02-at-gre-wo-staff.greenwich.ac.uk

No action is required on your part. Delivery attempts will continue for
some time, and this warning may be repeated at intervals if the message
remains undelivered. Eventually the mail delivery software will give up,
and when that happens, the message will be returned to you.

} From MAILER-DAEMON Fri Dec 31 19:00 CST 1999
Received: from alta.gre.ac.uk (alta.gre.ac.uk [193.60.48.95]) by Sparc5.Microscopy.Com (8.6.11/8.6.11) with ESMTP id TAA01169 for {Microscopy-request-at-sparc5.microscopy.com} ; Fri, 31 Dec 1999 19:00:01 -0600
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for Microscopy-request-at-sparc5.microscopy.com; Sat, 01 Jan 2000 01:00:25 +0000


-----BEGIN PGP SIGNED MESSAGE-----
Hash: SHA1

Thanks for the info. I understand what is going on now. sounds like a great opportunity.

I discussed compensation before and don't want to clutter up the list with a repeat. But I
would appreciate an off-list communication which helps me better understand how the
mechanics of SEM and its personnel work in the big picture. I get asked often how to
get into the field. Since I use my SEM for personal research and photography only, I am
not in academia or companys' formal research departments. If I sound ignorant about
this, it is because I am. I work with some high school and upper level pre-high school
students at times using my SEM and LMs. What can be done with the SEM and LMs
turns them on as much as it still does me today. I'm hopelessly hooked.

But I lack facts and figures about this type of career. Rather than speculating about it,
what do you careerists think? Is this a good career? What is involved in getting into
the career at various levels? And what compensation is commensurate at these levels?

All responses will be kept confidential. Use PGP if you need to.

gary g.




At 12:39 PM 1/1/00 , you wrote:
} sounds like a research grant to me. I don't believe the salary of faculty in Chemistry departments of most major colleges would be "soft money" requiring grant support. I commend the Society of Analytical Chemists for trying to encourage this bridge. 20K of seed money for a new line of research can make an important difference to a junior faculty member.
}
}
} } ---------------------------------------------------------------.
} }
} }
} } Hum....so for a 2000 hour year, this works out to be $10 per hour.
} } What a gift. And they probably want $30/hour of work in return.
} }
} } No matter how you package it, all of this babble does not measure
} } up to today's standards. Unless SEM, etc. is a obscure and
} } diminutive endeavor, I simply do not understand the cost-benefit
} } ratio. Maybe this is not an annual salary. OK. Is this in addition
} } to an existing income stream? Geeze, I hope it is the latter. But it
} } sounds like the position is on-site. So, the candidate gets a full time
} } job at McDonald's as well?
} }
} } All I can say is that I am glad and relieved that I do not have to
} } work and try to survive in this type of environment. Welcome to H-2 visas.
} }
} } gary g.
} }
} }
} } At 08:14 AM 12/31/99 , you wrote:
} } } ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } -----------------------------------------------------------------------.
} } }
} } }
} } } I just received this notification from the Society for Analytical Chemists
} } } of Pittsburgh. Perhaps it will provide an important start for a new chem
} } } prof who has an interest in expanding the use of microscopy and/or in
} } } walking across the new bridge between microscopy and spectroscopy:
} } }
} } } "Twenty-first annual Analytical Chemistry Starter Grant Award"
} } } The society for Analytical Chemists of Pittsburgh will award one grant of
} } } $20,000 to an assistant professor in the field of analytical chemistry.
} } } The purpose of this grant is to encourage high-quality, innovative research
} } } by a new analytical chemistry professor and to promote the training and
} } } development of graduate students in this field. Assistant professors who
} } } have accepted a US college or university appoint since December 31, 1996
} } } are eligible. Application forms available from:
} } } James Chadwick, Chairman
} } } Starter Grant Committee
} } } Society for Analytical Chemists of Pittsburgh
} } } 200 Penn Center Blvd., Suite 332
} } } Pittsburgh, PA 15235
} } } Ph: 1-800-825-3221, Xt. 208
} } } Fx: 412-825-3224
} } }
} } } Deadline for application receipt: February 29, 2000
} } } Award winner announced: May 1, 2000
} } }
} } }
} } } Best regards and welcome to the new millennium!
} } } Barbara Foster
} } } Consortium President
} } } Microscopy/Microscopy Education ...Educating microscopists for greater
} } } productivity.
} } }
} } } 125 Paridon Street Suite 102 Springfield, MA 01118
} } } PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com
} } } Visit our web site {http://www.MME-Microscopy.com/education}
} } } ******************************************************
} } } MME is America's first national consortium providing
} } } customized on-site workshops in all areas of
} } } microscopy, sample preparation, and image analysis.
}
} Thomas E. Phillips, Ph.D.
} Associate Professor of Biological Sciences
} Director, Molecular Cytology Core Facility
}
} 3 Tucker Hall
} Division of Biological Sciences
} University of Missouri
} Columbia, MO 65211-7400
} (573)-882-4712 (voice)
} (573)-882-0123 (fax)

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From daemon Sat Jan 01 17:50:59 2000



From: Nestor J. Zaluzec :      zaluzec-at-Sparc5.Microscopy.Com
Date: Sat, 1 Jan 2000 17:17:47 -0600
Subject: Re: PDP EDS systems after December 31st

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



A simple fix will be to set your date to the year 1972. Since
the days of the week for 1972 = 2000, 1973 = 2001, etc...

It should hold you for a few years (at least it works on my old PDP).

Nestor





From daemon Mon Jan 03 07:35:45 2000



From: Joseph Passero :      jp-at-spacelab.net
Date: Sun, 02 Jan 2000 23:04:00 -0500
Subject: WTB: Looking for a Manual, for an AO-860 Microtome....

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I am looking for a service and or operator's manual (an extra one you may have or a copy)
for an American Optics 860 sliding microtome.

Thank You

Best Regards

Joseph Passero

mailto:jp-at-spacelab.net



________________________________________________________
1stUp.com - Free the Web®
Get your free Internet access at http://www.1stUp.com



From daemon Mon Jan 03 17:30:19 2000



From: Joseph Passero :      jp-at-spacelab.net
Date: Mon, 03 Jan 2000 11:54:50 -0500
Subject: COURSE ANNOUCMENT - Polarized Light Microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I am posting this for the NY Microscopical Society.

This is a Very Good Course, at a Great Price.

Best Regards

Joseph Passero
mailto:jp-at-spacelab.net


New York Microscopical Society -- Course Announcement
====================================================

Bernard Friedman Memorial Workshop

Polarized Light Microscopy

April 8, 9, 15 & 16, 2000

An advanced course on polarized light microscopy which will cover the following topics:

The nature of polarized light

The origin and interpretation of interference colors

Birefringence and crystal orientation

The Indicatrix

Compensation and variable compensators

Interference figures and their interpretation

The workshop will consist of two consecutive weekends of lectures and hands on labs to cover
the theoretical and practical aspects of polarized light microscopy.

The course instructors include;

Jan Hinsch of Leica, Inc.

John Reffner of Trace Consulting

N.Y.M.S. Instructor Donald O'Leary.

WHEN: April 8, 9, 15 & 16, 2000 from 10 A.M. to 4 P.M.

WHERE:

New York Microscopical Society Facility
1244 McBride Avenue
West Paterson, NJ.

Phone (973) 812-8377

Web Site URL: http://www.nyms.org

(The facility has free parking and is accessible by public transportation, Information on
car pools and transportation will be provided.)

COST: $275 for N.Y.M.S. members, $295 for non-members (includes membership) Lunch and course
materials are included. Checks made out to N.Y.M.S.

WHO: advanced course for those who have completed "The Use of the Microscope" or are
experienced in microscopy and familiar with the theory of its use.

HOW: Register using the form below. Limited to the first 12 registrants.


For Further Information Contact

Donald O'Leary.

eMail: mailto:donoleary-at-worldnet.att.net

(201) 797 -8849 Voice Phone Number


--------------------------------------------------------------------------------------------------------------

Registration Form

Polarized Light Microscopy

N.Y.M.S. Member ______________________ ($275) Non-Member _______________($295)

Name ___________________________________________________________________

Address __________________________________________________________________

City __________________________________ State ____________________ Zip Code
________________

Phone (W) _______________________ (H) ______________________ eMail
_____________________________



________________________________________________________
1stUp.com - Free the Web®
Get your free Internet access at http://www.1stUp.com



From daemon Mon Jan 03 17:30:21 2000



From: Barbara Foster :      mme-at-map.com
Date: Mon, 03 Jan 2000 12:56:07 -0500
Subject: Re: Potential for expansion of microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Tom,

Thanks for your on-target observations ...and especially for getting Gary
into a more positive perspective!

Best regards,

Barbara Foster
Consortium President
Microscopy/Microscopy Education ...Educating microscopists for greater
productivity.

125 Paridon Street Suite 102 Springfield, MA 01118
PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com
Visit our web site {http://www.MME-Microscopy.com/education}
At 02:39 PM 1/1/00 -0600, Tom Phillips wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Mon Jan 03 17:30:21 2000



From: Lugosi1936-at-aol.com
Date: Mon, 3 Jan 2000 13:18:13 EST
Subject: Vickers Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

I am looking for any information (year of manufacture, company history, etc.)
about a Vickers Instruments, M1500974C microscope. I would especially like
to acquire a copy of the owners manual if possible. Please look at the
following pictures
for further identification.

{A HREF="http://members.aol.com/lugosi1936/vic1a.jpg"} http://members.aol.com/l
ugosi1936/vic1a.jpg {/A}
{A HREF="http://members.aol.com/lugosi1936/vic2.jpg"} http://members.aol.com/lu
gosi1936/vic2.jpg {/A}
{A HREF="http://members.aol.com/lugosi1936/vic3.jpg"} http://members.aol.com/lu
gosi1936/vic3.jpg {/A}

Thanks for you help,
Dana Grantham





From daemon Mon Jan 03 17:30:22 2000



From: Barbara Foster :      mme-at-map.com
Date: Mon, 03 Jan 2000 13:20:39 -0500
Subject: LM: Course Announcement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


American Chemical Society, "Applied Optical Microscopy"

3 days of exciting interactive lectures, lab, and demos on all aspects of
Light Microscopy, with a touch of video imaging
Learn how to
-match optics to your application
-interpret images from a variety of contrast techniques
-troubleshoot for artifacts and misinformation
-do simple measurement
-put a camera system on your microscope

New Orleans Hyatt Regency, Mar 10-12,2000
Tuition, books, coffee breaks: $895 for ACS members, $995 for non-members

While many of the examples used will be from materials science, this course
is NOT LIMITED TO CHEMISTS! Biologists are also welcome.

For a syllabus and enrollment information, visit MME's website:
www.MME-Microscopy.com/education (B. Foster is course coordinator)

Please reserve early. This course is given in conjunction with Pittcon,
the biggest analytical meeting in the country. Rooms fill up early.

Best regards .... and welcome to the positive side of Y2K!

Barbara Foster
Consortium President
Microscopy/Microscopy Education ...Educating microscopists for greater
productivity.

125 Paridon Street Suite 102 Springfield, MA 01118
PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com
Visit our web site {http://www.MME-Microscopy.com/education}



From daemon Mon Jan 03 18:31:48 2000



From: Mei Lie Wong :      wong-at-msg.ucsf.edu
Date: Mon, 3 Jan 2000 16:03:51 -0800
Subject: CHROMOSOME 5 (5P-)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} I am forwarding this from someone who sent this to me. If anyone has any
} ideas for this person, please contact him through his email address which
} is listed.
}
} ML
}
} } From: "rwinn" {rwinn-at-mweb.co.za}
} } To: {wong-at-msg.ucsf.edu}
} } Subject: CHROMOSOME 5 (5P-)
} } Date: Fri, 31 Dec 1999 07:21:48 +0200
} } MIME-Version: 1.0
} } X-Priority: 3
} }
} } DEAR, MEI LEI WONG
} }
} } MY NAME IS RENEY WINN AND I AM MAILING YOU FROM SOUTH AFRICA.
} } I AM 25 YRS OLD AND HAS A BABY BOY OF 14 MTHS.
} }
} } I FOUND OUT THAT HE HAS CRI-DU-CHAT SYNDROME WHICH IS ALSO CALLED CAT-CRY
} } SYNDROME. HE HAS A DELETION ON THE SHORT ARM OF CHROMOSOME 5. HIS
} } DELETION ON THE SHORT ARM IS FROM THE 14.2 MARK WHICH EXTENDS TO THE 15.
} } MARK.
} }
} } I KNOW THAT THIS IS A VERY RARE SYNDROME AND HAVE BEEN IN TOUCH WITH THE
} } SUPPORT GROUPS IN AUSTRALIA AND U.K. HOWEVER..
} } I STILL FEEL THAT I NEED MORE HELP FROM LABRORATRIES OR SCIENTISTS. I AM
} } TRYING TO FIND OUT MORE ABOUT THE SEVERITY OF MY SON'S CONDITION. I AM
} } TRYING TO FIND OUT HOW BADLY HE WILL BE AFFECTED BY HIS DELETION. I WOULD
} } JUST LIKE TO KNOW HOW HIS DELETION SIVERITY WOULD BE CLASIFIED AS. MAYBE
} } ON A SCALE OF 1-10 ONE AS BEING NONE SIVERE AND 10 AS MOST SEVERE. THIS
} } WAY I WOULD BE ABLE TO UNDERSTAND +- HOW SEVERE MY CHILD'S CONDITION IS. I
} } WOULD ALSO APPRECIATE IT IF YOU CAN TELL ME MORE OR LESS WHAT TO EXPECT
} } WITH HIS DELETION.
} }
} } MEI, IF THIS IS NOT SOMETHING YOU CAN HELP ME WITH, COULD YOU PLSE
} } FORWARD THIS MAIL TO SOMEONE WHO COULD PLSE. I AM VERY DESPERATE TO FIND
} } OUT MORE ABOUT THE SEVERITY OF MY CHILDS DELETION. THIS WOULD BE MUCH
} } APPRECIATED.
} }
} } BRGDS
} } RENEY WINN.
} } rwinn-at-mweb.co.z
Mei Lie Wong
Electron Microscope Laboratory
Department of Biochemistry
HHMI-UCSF
Ph. 415-476-4441 Fax 415-476-1902
http://util.ucsf.edu/agard/wong/index.html
email wong-at-msg.ucsf.edu



From daemon Tue Jan 04 18:52:22 2000



From: Mike Southwell :      mjsouth-at-flash.net
Date: Mon, 03 Jan 2000 18:44:05 -0800
Subject: BIO-Bacterial Growth Medium

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello all,

I know this is a little off the microscopy subject, but in the interest
of encouraging scientific exploration in a young person. I hope you will
indulge me.

I'm looking for materials for my daughter's science project. She is
testing the effectiveness of different mouthwashes in killing bacteria
found in the mouth. Her experiment matrix requires 12 petri dishes with
a bacterial growth medium. I can probably scrounge something to use as
petri dishes, but the growth medium is a problem. I'm not even sure
what it is properly called or what its makeup is. Is it something we
can easily purchase locally, or even better is there a recipe for making
a suitable substitute from ingredients found in the home?

Any and all suggestions will be greatly appreciated.

Michael Southwell
JEOL USA INC.
Austin, TX




From daemon Tue Jan 04 07:29:31 2000



From: Krzysztof Jan Huebner :      hubner-at-IOd.krakow.pl
Date: Tue, 4 Jan 2000 09:33:21 +0100 (MET)
Subject: Metal 2000 in the Ostrava 16-18 May 2000

Contents Retrieved from Microscopy Listserver Archives
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Good morning,

In the Ostrava, Czech 16th-18 May 2000 are organizing
"Metal 2000" - 9th international metallurigcal conference,

about conference look at http://www.tanger.cz/metal2000

best regards

Krzysztof Jan Huebner

{hubner-at-IOd.krakow.pl} :-)



From daemon Tue Jan 04 18:11:43 2000



From: Laurie Le Tarte :      lletarte-at-uamail.albany.edu
Date: Tue, 4 Jan 2000 10:06:47 +0000
Subject: Seeking applicants for a postdoctoral research postion

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Postdoctoral Research Associate

The New York State Center for Advanced Thin Film Technology
The University at Albany - State University at New York

The Center for Advanced Thin Film Technology at the University at
Albany - SUNY seeks applicants for a postdoctoral position with
established expertise in the area of analytical electron microscopy.
Demonstrated experience in the development and application of SEM
and/or TEM based analyses, including chemical analysis, is required to
achieve targeted technique development goals and to support an
expanding microscopy base at the Center. Experience with, or interest
in, scanning probe microscopy techniques is also desirable.
Candidates with a Ph.D. in applied physics, materials science and
engineering, chemistry or related fields are encouraged to apply.
This appointment is part of an ongoing multi-year investment in
personnel and new laboratory facilities for thin film technology
research areas at the Center.

The Center is a New York State sponsored high technology research and
development center with a $60M state-of-the-art infrastructure,
including class 1 clean rooms for back end IC manufacturing on 200 mm
wafers, and advanced processing, patterning and characterization
capabilities for single and multi-layered thin films. The Center is
currently assembling a 200 mm wafer facility for MEMS integration. In
addition, a new facility that will house a 25,000 ft2 class 1cleanroom
for 300 mm wafer pilot manufacturing and workforce training is
presently under construction.

Please send a letter of application including a summary of research
interests, curriculum vitae, and three letters of recommendation to:

William Harris, Director of Analytical Sciences
NYS Center for Advanced Thin Film Technology
CESTM B110
251 Fuller Road
Albany, NY 12203



The SUNY/Research Foundation is an Equal
Opportunity/Affirmative Action Employer. All
positions contingent upon availability of
funding.



From daemon Tue Jan 04 18:11:44 2000



From: Laurie Le Tarte :      lletarte-at-uamail.albany.edu
Date: Tue, 4 Jan 2000 10:30:36 +0000
Subject: Seeking applicants for an analytical support specialist

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Analytical Support Specialist

The New York State Center for Advanced Thin Film Technology
The University at Albany - State University at New York

The New York State Center for Advanced Thin Film Technology at the
University at Albany - SUNY is a fast growing, high technology
research and development program with a mission of supporting industry
and creating new jobs. This position will serve as the primary
infrastructure support person for the Center's scientific and
technical staff.

Job responsibilities include: performing routine maintenance and
calibration of a variety of surface spectroscopic and electron
microscopy instruments; performing analytical work with these
instruments; assisting students with the operation of this equipment;
diagnosing and repairing non-routine problems with the
instrumentation; and maintaining an adequate store of spare parts and
supplies.

The position requires: an associates degree in electronics, computer
science, or related technology and two to four years of directly
relevant experience; a working knowledge of high vacuum systems;
proficiency with related computer hardware/software; demonstrated
ability to work in a high energy, team oriented environment; and
excellent communication and analytical skills. Experience with surface
analysis and/or electron microscopy instrumentation is highly
desirable.

Please send a letter of application including a summary of research
interests, curriculum vitae, and three letters of recommendation to:

William Harris, Director of Analytical Sciences
NYS Center for Advanced Thin Film Technology
CESTM B110
251 Fuller Road
Albany, NY 12203

The SUNY/Research Foundation is an Equal
Opportunity/Affirmative Action Employer. All
positions contingent upon availability of
funding.



From daemon Tue Jan 04 18:11:43 2000



From: Walck. Scott D. :      walck-at-ppg.com
Date: Tue, 4 Jan 2000 10:05:11 -0500
Subject: Request for EELS data in EMSA Format -Windows Spectra Plotting pr

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear all,

I am working on a spectra plotting program in Visual Basic 6 that is just
about ready for general release. I was hoping to put it out there in time
for the M&M MM meeting.

It started out just as a program that would take EMSA formatted EELS data
from Gatan's ELP and convert them into an Excel compatible file so that I
bring the file to a PC. Then I got carried away and used it to try to learn
Visual Basic. Currently, the program will open and overlay up to five
spectra, plot them in linear, log or rescale them and print them. They can
also be copied to the clipboard and pasted in other applications. They can
be re-colored and a couple other things as well. There is even a help
file!

I am trying to make the program more general and make it completely
compatible with the EMMFF standard for all spectra, EDS and EELS alike.I
have a couple of requests for information.

1) I would like to have some EELS spectra of different elements in EMMFF
format to test it out and include in a distribution packet that I can put on
the MAS-LIB. I am particularly interested in transition metal oxides.
Please send them to me if you have them and I could put them in the
deployment file. Who knows, this could be a poor man's EELS atlas.

2) I would like to know how much interest there is in this program.

3) What features would you like in such a program. It doesn't do any
analysis yet, but it may in the future.

-Scott


Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)





From daemon Tue Jan 04 18:11:46 2000



From: Howard, Jean M. :      JMHoward-at-rmc.com
Date: Tue, 4 Jan 2000 10:48:26 -0500
Subject: SEM-Refurbishing multi-standards mount

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear listers,

I have a multi-element standards mount which over time has deteriorated and
become contaminated with salts. Three of the standards have fallen out of
the holder. The mount was made by C.M. Taylor Corp., Sunnyvale, Calif. in
1982, and it appears that this company no longer exists.
I am interested in finding someone to re-polish the mount and replace the
lost standards. Does anyone know of such a company/person?

Thanks in advance,

Jean M. Howard
Reynolds Metals Company
Materials Characterization-Electron Microscopy
E-mail: jmhoward-at-rmc.com
Office: 804.751.2554






From daemon Tue Jan 04 18:11:46 2000



From: Huggins, Bradley J :      HUGGINBJ-at-BP.com
Date: Tue, 4 Jan 2000 10:53:06 -0500
Subject: RE: SEM - epoxy mountants

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Tina,
I have not been reading all posts so you may have been provided with all the
help you needed, but I thought I would respond just in case...
We do a lot of work with similarly embedded specimens. The problem you have
is very familiar to me, and I believe you are right in your approach to
"Hold the samples in a vacuum for some period of time before putting them
into the scope" This is the best method that I have been able to come up
with. Assuming: 1) the resin is fully cured, 2) you have vacuum
infiltrated the resin into the specimen as thoroughly as practical, and 3)
you have minimized the size of high surface area specimens prior to
embedding, then there is not much more to try. In my experience the
outgassing is most often the result of contaminants within the porosity of
the specimen, and is not the result of the resin. If you still feel the
need to check this out further, you might try an embedding procedure using a
thermosetting resin (such as Bakelite) rather than a thermoplastic (epoxy).


If the sample prep involves polishing, then the source of the contamination
is probably the water or oil lubricant in that procedure. Minimize these
contaminants by preparing the smallest, and best vacuum infiltrated specimen
with as little porosity as possible. I do this by repetitive evacuation/N2
back filling (with heat if possible) prior to embedding. This "clears the
way" for better vacuum infiltration.

I have had polymerization problems with some resin/material combinations,
and I have usually overcome these by choosing a different resin polymer.
Epoxies and acrylics, for example, behave quite differently on various
substrates, so I will try LR White if I am having trouble with epoxy, and
visa versa. If you use LR White for the initial embedding, keep the
reaction volume small, and re-embed in a larger mount if necessary.
I do not know if there are any good references on the subject of vacuum
infiltration for porous materials, but these are the approaches I have
learned to take.
Good Luck!
Brad Huggins

} ----------
} From: Tina Carvalho[SMTP:tina-at-pbrc.hawaii.edu]
} Sent: Tuesday, December 28, 1999 2:23 PM
} To: Microscopy Listserver
} Subject: SEM - epoxy mountants
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hello, all-
}
} We have a Hitachi S-800 FESEM that is mostly used for biological
} specimens. However, recently we have had a number of planetary geologists
} looking at sections of meteorites mounted on epoxy resin. About the time
} they started using the scope, we started having a number of problems that
} suggest that we are getting some outgassing and contamination. I'm not
} surprised; this happened before when looking at fish ear bones in similar
} resin, despite the resin manufacturer's clain their product was completely
} stable in the high vacuum and under the beam.
}
} My question is for those of you who routinely look at such samples: What
} do you do to minimize the potential problems? Hold the samples in a
} vacuum for some period of time before putting them into the scope? Paint
} the exposed epoxy areas with carbon paint or similar? For the biological
} samples we only require that the specimens be held over dessicant
} overnight, but I suspect that more heroic measures must be taken for these
} samples.
}
} Happy New Year to all!
}
} Tina
}
} **************************************************************************
} **
} * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu
} *
} * Biological Electron Microscope Facility * (808) 956-6251
} *
} * University of Hawaii at Manoa *
} http://www.pbrc.hawaii.edu/bemf*
} **************************************************************************
} **
}
}



From daemon Tue Jan 04 20:13:28 2000



From: Lehman, Ann :      Ann.Lehman-at-trincoll.edu
Date: Tue, 4 Jan 2000 15:11:21 -0500
Subject: Philips400 LaB6 & Supplies

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Can someone suggest a reliable manufacturer for LaB6 filaments? I need the
sturdiest, most poor-vacuum-tolerant variety for a Philips EM400T in a
student lab! and I figure that users know best which filaments perform well
in the real world.

Also, I am looking for a source for Philips bulk-sample mounts, the type
used to carry SEM samples for the 6485/STEM system. (I need the mounts, not
the specimen rod.) I need both low-background carbon (preferred) or
beryllium for EDS, as well as standard copper carriers. My usual sources
tell me they have not stocked these items for years. Maybe someone has some
sitting unused in a cabinet somewhere?? or knows of a current supplier.

Offlist replies preferred, so as not to clog up the works... will summarize
and send info to others who are interested.

Thanks for your help.

Ann Hein-Lehman
Trinity College
Hartford, CT
860-297-4289
ann.lehman-at-trincoll.edu




From daemon Tue Jan 04 18:42:23 2000



From: Karen Zaruba :      kszaruba-at-MMM.COM
Date: Tue, 04 Jan 2000 14:55:19 -0600
Subject: Re: Request for EELS data in EMSA Format -Windows Spectra Plotting

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have a side question spurred by Scott Walck's post. Is there a simple low
cost program already available that would just allow me to view and print EMSA
format spectra under a Windows PC environment? I found some info. in the
archives regarding the EMMPDL site and the EMMFF software. I even got so far as
to download the sourcecode. But, I don't have a compiler to turn it into an
executable. Even so, I'm not sure from the documentation whether it will do
what I want. Also I came across NIST's DTSA but that's only for Mac.

Any suggestions out there? And if not, then to Scott yes there is some interest
from myself!
Thanks,
Karen Zaruba

"Walck. Scott D." wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Dear all,
}
} I am working on a spectra plotting program in Visual Basic 6 that is just
} about ready for general release. I was hoping to put it out there in time
} for the M&M MM meeting.
}
} It started out just as a program that would take EMSA formatted EELS data
} from Gatan's ELP and convert them into an Excel compatible file so that I
} bring the file to a PC. Then I got carried away and used it to try to learn
} Visual Basic. Currently, the program will open and overlay up to five
} spectra, plot them in linear, log or rescale them and print them. They can
} also be copied to the clipboard and pasted in other applications. They can
} be re-colored and a couple other things as well. There is even a help
} file!
}
} I am trying to make the program more general and make it completely
} compatible with the EMMFF standard for all spectra, EDS and EELS alike.I
} have a couple of requests for information.
}
} 1) I would like to have some EELS spectra of different elements in EMMFF
} format to test it out and include in a distribution packet that I can put on
} the MAS-LIB. I am particularly interested in transition metal oxides.
} Please send them to me if you have them and I could put them in the
} deployment file. Who knows, this could be a poor man's EELS atlas.
}
} 2) I would like to know how much interest there is in this program.
}
} 3) What features would you like in such a program. It doesn't do any
} analysis yet, but it may in the future.
}
} -Scott
}
} Scott D. Walck, Ph.D.
} PPG Industries, Inc.
} Glass Technology Center
} Guys Run Rd. (packages)
} P. O. Box 11472 (letters)
} Pittsburgh, PA 15238-0472
}
} Walck-at-PPG.com
}
} (412) 820-8651 (office)
} (412) 820-8161 (fax)

--
Karen S. Zaruba kszaruba-at-mmm.com
3M Company, St. Paul, MN





From daemon Tue Jan 04 19:18:31 2000



From: Nestor J. Zaluzec :      zaluzec-at-Sparc5.Microscopy.Com
Date: Tue, 4 Jan 2000 19:13:46 -0600
Subject: Spectra in MSA/MSA Format How to Plot...

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



As one of the co-authors of the MSA/MAS File format we
had a goal to make the format simple enough to be
useable in even a simple spreadsheet program.

A number of the major manufacturer's allow you to
translate their data files into this format directly
from their application programs. To use the data
in a spreadsheet, simply specify dual column (x,y)
format for the output file and
then you can import the data files directly into
a MS Excel spreadsheet, or even better a data
graphing program like KaleidaGraph (which runs
on both Mac's & PC's). Then plot to your hearts content.



Nestor
Your Friendly Neighborhood SysOp.





From daemon Tue Jan 04 20:13:27 2000



From: McLean, Dorrance :      dmclea-at-sandia.gov
Date: Tue, 4 Jan 2000 18:34:58 -0700
Subject: Mat: Cadmium Zinc Telluride thin foils

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,
I've been working on thin foils of CZT and I'm looking for some suggestions
to help eliminate artifacts created by ion milling. So far I have polished
side one and etched (to remove polishing damage) with bromine-methanol and
then dimpled side two, ending with 0.1um alumina. I have been successful
obtaining a final sample thickness of about 8 to 10 microns. However, when
I put the samples into the PIPs (Gatan) to complete the thinning process I'm
seeing beam damage. I was hoping that someone might have a different
approach or suggestion that would eliminate the beam damage.
Thanks for your help.
Dorrance




From daemon Wed Jan 05 08:22:43 2000



From: Palatsides, Manuela :      m.palatsides-at-pmci.unimelb.edu.au
Date: Wed, 5 Jan 2000 13:23:00 +1100
Subject: TEM: Staining yeast

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I have an investigator who would like to stain just the cell wall of the
yeast S.cerevisae to distinguish it from the cell membrane at the EM level.
Any tips or references willbe appreciated.
Many thanks

Manuela Palatsides
Electron Microscopy
Peter MacCallum Cancer Institute
Locked Bag#1
A'Beckett Street
Melbourne 3000

Telephone: 03 96561244
Fax: 03 96561411
Email: m.palatsides-at-pmci.unimelb.edu.au





From daemon Wed Jan 05 08:22:49 2000



From: Nelson+ Conti :      NelsonC51-at-excite.com
Date: Tue, 4 Jan 2000 21:59:47 -0800 (PST)
Subject: Re: BIO-Bacterial Growth Medium

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html





On Mon, 03 Jan 2000 18:44:05 -0800, Mike Southwell wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hello all,
}
} I know this is a little off the microscopy subject, but in the interest
} of encouraging scientific exploration in a young person. I hope you will
} indulge me.
}
} I'm looking for materials for my daughter's science project. She is
} testing the effectiveness of different mouthwashes in killing bacteria
} found in the mouth. Her experiment matrix requires 12 petri dishes with
} a bacterial growth medium. I can probably scrounge something to use as
} petri dishes, but the growth medium is a problem. I'm not even sure
} what it is properly called or what its makeup is. Is it something we
} can easily purchase locally, or even better is there a recipe for making
} a suitable substitute from ingredients found in the home?
}
} Any and all suggestions will be greatly appreciated.
}
} Michael Southwell
} JEOL USA INC.
} Austin, TX
}
}
Michael -
I'm sure that any bacterial growth medium requries agar or, at home,
gelatin. If you were considering making it at home, then buy some clear
gelatin. You'll need to boil water first, though, and then add the gelatin
to make it liquid. Before it cools, pour the gelatin along with the
apprpriate growth medium into the petri plates (typically, a plate holds
about 10 ml of medium). This substance (err, the gelatin) acts primarily as
a hardener for the actual medium. It is generally considered to be
non-nutritive for most bacteria, although I believe that some may consider
it to be nutritious (spelling ?). As for the main nutrients ... I've been
dealing primarily with protozoa, but I'm pretty sure that bacteria can grow
on an extract of boiled lettuce or even wheat grains, etc.
I cannot tell you from my own experience, however, whether a boiled extract
in combination with gelatin does indeed work as a suitable growth medium,
only that I've heard that gelatin works and that, in my experience, a boiled
extract works well for supporting bacterial growth for protozoa.
If, instead, you have access to scientific catalogs, then it's fairly easy
simply to order proteose peptone, glucose, and agar. The peptone is a
standard component of typical "nutrient agar" plates that, I believe, can be
bought commercially, and the glucose may be needed as a sugar source and
carbon source (?). The agar serves to harden the medium much like gelatin is
supposed to.
I hope this helps.
Nelson Conti
[a graduate student formerly from San Francisco State University with a M.A.
degree in microbiology & a B. A. degree from UC Davis in Bacteriology ]





_______________________________________________________
Visit Excite Shopping at http://shopping.excite.com
The fastest way to find your Holiday gift this season




From daemon Wed Jan 05 08:22:53 2000



From: Ken Tiekotter :      tiekotte-at-up.edu
Date: Wed, 5 Jan 2000 00:07:03 -0800 (PST)
Subject: Re: SEM - epoxy mountants

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear tina and others,
My experience with samples you have described makes me head for the
nearest exit! However, I have looked at samples such as expanded teflon,
and vascular castings mounted on nothing more than carbon sticky tabs.
Frequently I have clientes who do not want samples to be gold coated and
consequently, charging is an issue.

I frequently examine this specimens at an extremely reduced kV and adjust
the spot size and working distance to help in obtaining the best
resolution. The parameters will be different for a FESEM: I am using a
tungsten system. I would think a coating of colloidal carbon or silver to
ground would help under any circumstances. Any thoughts? Good luck!

Ken Tiekotter




On Tue, 28 Dec 1999, Tina Carvalho wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hello, all-
}
} We have a Hitachi S-800 FESEM that is mostly used for biological
} specimens. However, recently we have had a number of planetary geologists
} looking at sections of meteorites mounted on epoxy resin. About the time
} they started using the scope, we started having a number of problems that
} suggest that we are getting some outgassing and contamination. I'm not
} surprised; this happened before when looking at fish ear bones in similar
} resin, despite the resin manufacturer's clain their product was completely
} stable in the high vacuum and under the beam.
}
} My question is for those of you who routinely look at such samples: What
} do you do to minimize the potential problems? Hold the samples in a
} vacuum for some period of time before putting them into the scope? Paint
} the exposed epoxy areas with carbon paint or similar? For the biological
} samples we only require that the specimens be held over dessicant
} overnight, but I suspect that more heroic measures must be taken for these
} samples.
}
} Happy New Year to all!
}
} Tina
}
} ****************************************************************************
} * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
} * Biological Electron Microscope Facility * (808) 956-6251 *
} * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
} ****************************************************************************
}
}
}




From daemon Wed Jan 05 08:22:57 2000



From: TQ23TEAM-at-LHT.DLH.DE
Date: Wed, 5 Jan 2000 10:30:53 +0100
Subject: Re: SEM-Refurbishment multi-standard mount

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear listers,

I have a multi-element standards mount which over time has deteriorated and
become contaminated with salts. Three of the standards have fallen out of
the holder. The mount was made by C.M. Taylor Corp., Sunnyvale, Calif. in
1982, and it appears that this company no longer exists.
I am interested in finding someone to re-polish the mount and replace the
lost standards. Does anyone know of such a company/person?

Thanks in advance,

Jean M. Howard
Reynolds Metals Company
Materials Characterization-Electron Microscopy
E-mail: jmhoward-at-rmc.com
Office: 804.751.2554

Dear Jean,

Plano W. Plannet GmbH is a supplier for electron microscopy. This company
offers refurbishment of SEM standards.
The company is situated in Germany.

Address: Ernst-Befort-Str.12
D-35578 Wetzlar

e-mail: plano-at-t-online.de, or plano-at-plano-em.com
http://www.plano-em.com

You might contact them to see if they can help you with your problem.

best regards
Mit freundlichen Gr٤en

Detlef Warmbold
Geb. 381 Raum 2152
Tel. / Fax +49/40-5070-3962/1411
E-mail TQ23Team-at-LHT.DLH.DE



From daemon Wed Jan 05 08:53:23 2000



From: Laurie Le Tarte :      lletarte-at-uamail.albany.edu
Date: Wed, 5 Jan 2000 09:44:01 +0000
Subject: Seeking applicants for a postdoctoral research position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Postdoctoral Research Associate

The New York State Center for Advanced Thin Film Technology
The University at Albany - State University at New York

The Center for Advanced Thin Film Technology at the University at
Albany - SUNY seeks applicants for a postdoctoral position with
established expertise in the area of analytical electron microscopy.
Demonstrated experience in the development and application of SEM
and/or TEM based analyses, including chemical analysis, is required to
achieve targeted technique development goals and to support an
expanding microscopy base at the Center. Experience with, or interest
in, scanning probe microscopy techniques is also desirable.
Candidates with a Ph.D. in applied physics, materials science and
engineering, chemistry or related fields are encouraged to apply.
This appointment is part of an ongoing multi-year investment in
personnel and new laboratory facilities for thin film technology
research areas at the Center.

The Center is a New York State sponsored high technology research and
development center with a $60M state-of-the-art infrastructure,
including class 1 clean rooms for back end IC manufacturing on 200 mm
wafers, and advanced processing, patterning and characterization
capabilities for single and multi-layered thin films. The Center is
currently assembling a 200 mm wafer facility for MEMS integration. In
addition, a new facility that will house a 25,000 ft2 class 1cleanroom
for 300 mm wafer pilot manufacturing and workforce training is
presently under construction.

Please send a letter of application including a summary of research
interests, curriculum vitae, and three letters of recommendation to:

William Harris, Director of Analytical Sciences
NYS Center for Advanced Thin Film Technology
CESTM B110
251 Fuller Road
Albany, NY 12203

The SUNY/Research Foundation is an Equal
Opportunity/Affirmative Action Employer. All
positions contingent upon availability of
funding.



From daemon Wed Jan 05 09:03:24 2000



From: Laurie Le Tarte :      lletarte-at-uamail.albany.edu
Date: Wed, 5 Jan 2000 09:47:27 +0000
Subject: Seeking applicants for an analytical support position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Analytical Support Specialist

The New York State Center for Advanced Thin Film Technology
The University at Albany - State University at New York

The New York State Center for Advanced Thin Film Technology at the
University at Albany - SUNY is a fast growing, high technology
research and development program with a mission of supporting industry
and creating new jobs. This position will serve as a primary
infrastructure support person for the Center's scientific and
technical staff.

Job responsibilities include: performing routine maintenance and
calibration of a variety of surface spectroscopic and electron
microscopy instruments; performing analytical work with these
instruments; assisting students with the operation of this equipment;
diagnosing and repairing non-routine problems with the
instrumentation; and maintaining an adequate store of spare parts and
supplies.

The position requires: an associates degree in electronics, computer
science or related technology and two to four years of directly
relevant experience; a working knowledge of high vacuum systems;
proficiency with related computer hardware/software; demonstrated
ability to work in a high energy, team oriented environment; and
excellent communication and analytical skills. Experience with surface
analysis and/or electron microscopy instrumentation is highly
desirable.

Please send a letter of application including a summary of research
interests, curriculum vitae, and three letters of recommendation to:

William Harris, Director of Analytical Sciences
NYS Center for Advanced Thin Film Technology
CESTM B110
251 Fuller Road
Albany, NY 12203

The SUNY/Research Foundation is an Equal
Opportunity/Affirmative Action Employer. All
positions contingent upon availability of
funding.



From daemon Wed Jan 05 08:23:13 2000



From: Geoff Williams :      Geoffrey.Lloyd.Williams-at-cmich.edu
Date: Wed, 05 Jan 2000 08:30:43 -0500
Subject: GEN: ATTN: Lab Managers W/SEM&EDS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have a question for all of the SEM microscope labs with EDS.

What is the percent of Biological use that the EDS gets in your
facility?

This is going to be my first semester teaching a Biological EDS class.
The facility here sees very little, if any, biological EDS projects. It
is used here as in other places I am familiar with nearly 100% non
biological applications.

I would like as many responses as possible so I can give a
representative summary to the students in the class. A short reply
directly to me ( Geoffrey.Lloyd.Williams-at-cmich.edu or willi1gl-at-cmich.edu
) would be much appreciated. A rough % bio use, most popular bio
samples (plants or animals, type of organisms . . .) and most common
analysis (Quantitative, Qualitative, Dot mapping. . .).

Thank you in advance. If there is sufficient interest I would be
willing to put a summary up on the list for all.

-Geoff W.

--
Geoff Williams

Electron Microscope Facility Supervisor
Biology Department
Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859

Geoffrey.Lloyd.Williams-at-cmich.edu
517 774-3576
517 774-3462 (fax)





From daemon Wed Jan 05 08:53:22 2000



From: Gillmeister, Russ :      RGillmeister-at-sdms.usa.xerox.com
Date: Wed, 5 Jan 2000 09:15:28 -0500
Subject: Re: SEM - epoxy mountants

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ken, If you have a vacuum evaporator Ken, I would try indirect carbon. Most
metals will evaporate directionally only. Carbon will also evaporate
indirectly (around corners) although at a reduced thickness. The advantage
of this type of evaporation is the carbon will coat very convoluted surfaces
which are not line of site. Another advantage is the carbon will add very
little structure to your sample at high magnifications. Additionally heating
of the sample can be reduced due to shading of the source from the sample.
One caveat is, as you know, carbon is a very inefficient secondary producer
and the carbon will not have the efficiency of gold. Indirect carbon, with a
proper thickness, will prevent charging though. Just put a line of site
shield between your source and sample while keeping it as small as possible.
Good luck. Russ Gillmeister, Xerox

-----Original Message-----
} From: Ken Tiekotter [mailto:tiekotte-at-up.edu]
Sent: Wednesday, January 05, 2000 3:07 AM
To: Tina Carvalho
Cc: Microscopy Listserver


Dear tina and others,
My experience with samples you have described makes me head for the
nearest exit! However, I have looked at samples such as expanded teflon,
and vascular castings mounted on nothing more than carbon sticky tabs.
Frequently I have clientes who do not want samples to be gold coated and
consequently, charging is an issue.

I frequently examine this specimens at an extremely reduced kV and adjust
the spot size and working distance to help in obtaining the best
resolution. The parameters will be different for a FESEM: I am using a
tungsten system. I would think a coating of colloidal carbon or silver to
ground would help under any circumstances. Any thoughts? Good luck!

Ken Tiekotter




On Tue, 28 Dec 1999, Tina Carvalho wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hello, all-
}
} We have a Hitachi S-800 FESEM that is mostly used for biological
} specimens. However, recently we have had a number of planetary geologists
} looking at sections of meteorites mounted on epoxy resin. About the time
} they started using the scope, we started having a number of problems that
} suggest that we are getting some outgassing and contamination. I'm not
} surprised; this happened before when looking at fish ear bones in similar
} resin, despite the resin manufacturer's clain their product was completely
} stable in the high vacuum and under the beam.
}
} My question is for those of you who routinely look at such samples: What
} do you do to minimize the potential problems? Hold the samples in a
} vacuum for some period of time before putting them into the scope? Paint
} the exposed epoxy areas with carbon paint or similar? For the biological
} samples we only require that the specimens be held over dessicant
} overnight, but I suspect that more heroic measures must be taken for these
} samples.
}
} Happy New Year to all!
}
} Tina
}
}
****************************************************************************
} * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu
*
} * Biological Electron Microscope Facility * (808) 956-6251
*
} * University of Hawaii at Manoa *
http://www.pbrc.hawaii.edu/bemf*
}
****************************************************************************
}
}
}



From daemon Wed Jan 05 10:33:07 2000



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Wed, 5 Jan 2000 10:15:07 -0600
Subject: SEM: Sputter coating

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

Due to some recent high-resolution requirements in our lab, I find myself
having to go back to Sputter Coating 101 (after years of just putting
specimens in the coater and turning it on without a second thought!). We
find ourselves in need of very thin coatings with as little structure as
possible, in order to image samples down in the nanometer range.

We have a chromium coater, but often need to revisit samples days or weeks
after the initial coating. The oxidation problem rears its ugly head. We
intend to purchase a platinum target, but don't yet have one, so we're
experimenting with our venerable Au/Pd coater.

My questions are:

1) I seem to remember a string on this listserver suggesting that lower
deposition currents yield finer coating structure. Is this right? Does a
low deposition current for a longer time yield a finer coating than a higher
current for a shorter time? (I'm running some tests to check this, but
would be very interested in others' experiences, too.)

2) Deposition current can be controlled by the initial current setting
(i.e., the knob on the machine) and by the argon flow through the chamber.
Is there any difference in the coating when adjusting the deposition current
by either of these two ways?

3) Charts I have seen indicate that deposition current is directly
proportional to coating rate. Is the same true for coating time? I.e., is a
one minute coating twice as thick as a 30 sec. coating? It would seem so
intuitively, but you know what they say about the word "assume".

My apologies if these are very basic questions, but, like I said, back to SC
101!

I'll be happy to summarize the responses for anyone who is interested.

Thanks!

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine Bldg.
University of Missouri
Columbia, MO 65211
(573)882-8304
tindallr-at-missouri.edu
http://www.biotech.missouri.edu/emc/




From daemon Wed Jan 05 10:33:07 2000



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Wed, 05 Jan 2000 08:30:38 -0800
Subject: Re: Mat: Cadmium Zinc Telluride thin foils

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Mike,

You can probably use Agar, this is commonly used to grow yeast cultures for
brewing. This is a vegetable based gelatin like substance. You can buy it
at some organic type stores in the bulk section (you don't need much
probably 10 tbsp) it costs about $100/ounce.

I don't remember the exact proportions but I think you use something like
1-3 tsp per 2cups of water and 1 tbsp of a complex sugar (brown sugar will
probably be fine). You just get it al boiling for 5-10 min to sterilize it
and pour into sterile glass dishes WITH LIDS. I use small Ball jars. Put
it in the fridge and it should harden in 10-15 min. If not, then my
proportions are off.

After it is hard, just wipe some saliva on the media and watch the green and
red goo grow.

Good luck
-andrew

----- Original Message -----
} From: Mike Southwell {mjsouth-at-flash.net}
To: {microscopy-at-sparc5.microscopy.com}
Sent: Monday, January 03, 2000 7:44 PM


Dear Dorrance,
It seems to me that the etching step is redundant. The ion-milling should
remove any polishing deformation and the etching will introduce surface
profiling. I recall that the CZT is very soft and may require some
modification to the ion beam voltage or current to reduce damage. Sounds
like you are close to getting good results.
At 06:34 PM 1/4/00 -0700, you wrote:
}
} Hi,
} I've been working on thin foils of CZT and I'm looking for some suggestions
} to help eliminate artifacts created by ion milling. So far I have polished
} side one and etched (to remove polishing damage) with bromine-methanol and
} then dimpled side two, ending with 0.1um alumina. I have been successful
} obtaining a final sample thickness of about 8 to 10 microns. However, when
} I put the samples into the PIPs (Gatan) to complete the thinning process I'm
} seeing beam damage. I was hoping that someone might have a different
} approach or suggestion that would eliminate the beam damage.
} Thanks for your help.
} Dorrance
}
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca




From daemon Wed Jan 05 11:42:58 2000



From: Donna Wagahoff :      DWAGAHOFF-at-siumed.edu
Date: Wed, 5 Jan 2000 11:08:12 -0600
Subject: osmium's stability in a plastic container

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have always kept our osmium solutions in glass containers. However, we
are in the process of evaluating our safety procedures and discussed the
idea of increasing the safety of the transport of osmium from the
refrigerator to the fume hood by putting the osmium solution in plastic
containers. (If they are dropped, they would not break and cause the danger
of a spill outside the hood.)
Does anyone have experience with osmium stored in plastic? Any comments
about this particular subject or any of your safety with osmium procedures
are welcomed.
Thanks.

Donna Wagahoff
SIU School of Medicine
PO Box 19627
Springfield, IL 62794-9627
217-782-0898
fax217-524-3227



From daemon Wed Jan 05 12:12:54 2000



From: Mike Dalbey :      dalbey-at-biology.ucsc.edu
Date: Wed, 05 Jan 2000 10:07:45 -0800
Subject: Re: BIO-Bacterial Growth Medium

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Mike,

This experiment is very popular in the science fair circuit; it is only
slightly less popular than "What kind of music do plants like best?".
Moreover (as a topic RELEVANT TO THE LIST) van Leeuwenhoek showed more than
300 years ago, BY MICROSCOPY, that the experiment as usaully performed is
invalid as a test of mouthwash efficacy in situ. You might want to consult
his observations on the topic.

Prepared agar media can be purchased from educational scientific supply
houses such as Carolina Biologicals or Ward's, both of which maintain web
sites. They both offer "instant" formulations designed for preparation of
Petri dishes without autoclaving. You would want a medium capable of
supporting growth of Streptococcus species, which are an important
component of the oral microflora. The streptococci are somewhat fastidious
in their nutritional requirements. Therefore, select something rich in
organic nutrients such as amino acids. Tryptic Soy Medium would probably
work best. The proteose peptone/glucose composition suggested by a previous
respondent would also probably work. Lettuce or wheat grain extract would
probably not give satisfactory results.If you wish to make your own medium
at home from local sources, I suggest table sugar, beef bullion (more
concentrated than in culinary use) and agar (often available at health food
stores). A pressure cooker would help to ensure sterility during
preparation. Be advised that many of the bacteria in the oral cavity are
obligate anaerobes; they will not grow on any medium if it is in contact
with the atmosphere.

Best wishes for the success of your daughter's work!
Mike Dalbey
Biology Dept.
University of California
Santa Cruz, CA 95064

831-459-3674



From daemon Wed Jan 05 13:12:48 2000



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Wed, 05 Jan 2000 13:34:49 -0500
Subject: Fine SEM coating

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Randy Tindall wrote:
=================================================
{snip}
We find ourselves in need of very thin coatings with as little structure
as possible, in order to image samples down in the nanometer range.

We have a chromium coater, but often need to revisit samples days or weeks
after the initial coating. The oxidation problem rears its ugly head. We
intend to purchase a platinum target, but don't yet have one, so we're
experimenting with our venerable Au/Pd coater.

{snip}
===================================================
One (maybe the only) solution to the problem is to coat with osmium metal in
the osmium plasma coater. Pt grain size can be on the same size magnitude
as your nanostructures. You can get information about this coater on our
website at URL
http://www.2spi.com/catalog/osmi-coat.html

One does not need to worry about grain size because it is an (apparently
completely) amorphous layer and since it is a precious group metal, it has
the intertness of Au and Pt and will essentially last forever.

The physics of the process is explained in the US Patent #5855682 which can
be clicked to from the above URL.

We would be happy to coat some demo samples for you in our demo unit at any
time, just let us know.

Disclaimer: SPI Supplies distributes the line of Osmium Plasma Coaters,
manufactured by Nippon Laser and Electronics Ltd.

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.spi.cc
########################
============================================



From daemon Wed Jan 05 13:12:48 2000



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Wed, 05 Jan 2000 13:34:49 -0500
Subject: Fine SEM coating

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Randy Tindall wrote:
=================================================
{snip}
We find ourselves in need of very thin coatings with as little structure
as possible, in order to image samples down in the nanometer range.

We have a chromium coater, but often need to revisit samples days or weeks
after the initial coating. The oxidation problem rears its ugly head. We
intend to purchase a platinum target, but don't yet have one, so we're
experimenting with our venerable Au/Pd coater.

{snip}
===================================================
One (maybe the only) solution to the problem is to coat with osmium metal in
the osmium plasma coater. Pt grain size can be on the same size magnitude
as your nanostructures. You can get information about this coater on our
website at URL
http://www.2spi.com/catalog/osmi-coat.html

One does not need to worry about grain size because it is an (apparently
completely) amorphous layer and since it is a precious group metal, it has
the intertness of Au and Pt and will essentially last forever.

The physics of the process is explained in the US Patent #5855682 which can
be clicked to from the above URL.

We would be happy to coat some demo samples for you in our demo unit at any
time, just let us know.

Disclaimer: SPI Supplies distributes the line of Osmium Plasma Coaters,
manufactured by Nippon Laser and Electronics Ltd.

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.spi.cc
########################
============================================



From daemon Wed Jan 05 12:42:51 2000



From: Tom Phillips :      PhillipsT-at-missouri.edu
Date: Wed, 5 Jan 2000 12:41:26 -0600
Subject: Re: osmium's stability in a plastic container

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Agar can be purchased from some Asian stores at much cheaper rate if you can find them. It is made into strands, so, don't expect powder form.



Ann Fook Yang
EM Unit,
Eastern Cereal and Oilseed Research Centre,
Rm 2091, K.W. Neatby Bldg.,
Central Experimental Farm,
Ottawa, Ontario, Canada K1A 0C6

Phone: 613-759-1638
Fax; 613-759-1701

} } } "Andrew Cahill" {cahill-at-colorado.edu} 01/05 11:15 AM } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Mike,

You can probably use Agar, this is commonly used to grow yeast cultures for
brewing. This is a vegetable based gelatin like substance. You can buy it
at some organic type stores in the bulk section (you don't need much
probably 10 tbsp) it costs about $100/ounce.

I don't remember the exact proportions but I think you use something like
1-3 tsp per 2cups of water and 1 tbsp of a complex sugar (brown sugar will
probably be fine). You just get it al boiling for 5-10 min to sterilize it
and pour into sterile glass dishes WITH LIDS. I use small Ball jars. Put
it in the fridge and it should harden in 10-15 min. If not, then my
proportions are off.

After it is hard, just wipe some saliva on the media and watch the green and
red goo grow.

Good luck
-andrew

----- Original Message -----
} From: Mike Southwell {mjsouth-at-flash.net}
To: {microscopy-at-sparc5.microscopy.com}
Sent: Monday, January 03, 2000 7:44 PM


Agar can be purchased from some Asian stores at much cheaper rate if you can find them. It is made into strands, so, don't expect powder form.



Ann Fook Yang
EM Unit,
Eastern Cereal and Oilseed Research Centre,
Rm 2091, K.W. Neatby Bldg.,
Central Experimental Farm,
Ottawa, Ontario, Canada K1A 0C6

Phone: 613-759-1638
Fax; 613-759-1701

} } } "Andrew Cahill" {cahill-at-colorado.edu} 01/05 11:15 AM } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Mike,

You can probably use Agar, this is commonly used to grow yeast cultures for
brewing. This is a vegetable based gelatin like substance. You can buy it
at some organic type stores in the bulk section (you don't need much
probably 10 tbsp) it costs about $100/ounce.

I don't remember the exact proportions but I think you use something like
1-3 tsp per 2cups of water and 1 tbsp of a complex sugar (brown sugar will
probably be fine). You just get it al boiling for 5-10 min to sterilize it
and pour into sterile glass dishes WITH LIDS. I use small Ball jars. Put
it in the fridge and it should harden in 10-15 min. If not, then my
proportions are off.

After it is hard, just wipe some saliva on the media and watch the green and
red goo grow.

Good luck
-andrew

----- Original Message -----
} From: Mike Southwell {mjsouth-at-flash.net}
To: {microscopy-at-sparc5.microscopy.com}
Sent: Monday, January 03, 2000 7:44 PM


I store my osmium in a glass bottle with plastic cap (Schott bottle -
orange cap - commonly used for tissue culture work). The inside
portion of the cap turns black quite rapidly but the solution is
stable for months at room temperature. I store this glass bottle
inside an aluminum-foiled plastic container in my fume hood at room
temp. The clear plastic of this outer container gets translucent
black within weeks no matter how carefully I seal the glass bottle.
I think the storage of osmium in any single container (except a
sealed ampule) in the refrigerator is foolish and unnecessary. Due
to University regulations, we are required to store our aldehyde
fixatives and other toxic chemicals inside the refrigerator in
"secondary containment" containers. We keep the glass bottles of
aldehydes in small plastic containers and transport them this way to
the fume hood for use.

}
}
}
} We have always kept our osmium solutions in glass containers. However, we
} are in the process of evaluating our safety procedures and discussed the
} idea of increasing the safety of the transport of osmium from the
} refrigerator to the fume hood by putting the osmium solution in plastic
} containers. (If they are dropped, they would not break and cause the danger
} of a spill outside the hood.)
} Does anyone have experience with osmium stored in plastic? Any comments
} about this particular subject or any of your safety with osmium procedures
} are welcomed.
} Thanks.
}
} Donna Wagahoff
} SIU School of Medicine
} PO Box 19627
} Springfield, IL 62794-9627
} 217-782-0898
} fax217-524-3227

Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)



From daemon Wed Jan 05 13:22:45 2000



From: William Tivol :      tivol-at-wadsworth.org
Date: Wed, 05 Jan 2000 14:16:01 -0500
Subject: Re: osmium's stability in a plastic container

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Donna Wagahoff wrote:

} We have always kept our osmium solutions in glass containers. However, we
} are in the process of evaluating our safety procedures and discussed the
} idea of increasing the safety of the transport of osmium from the
} refrigerator to the fume hood by putting the osmium solution in plastic
} containers. (If they are dropped, they would not break and cause the danger
} of a spill outside the hood.)
} Does anyone have experience with osmium stored in plastic? Any comments
} about this particular subject or any of your safety with osmium procedures
} are welcomed.

Dear Donna,
How about putting the glass containers inside plastic containers?
There are also padded and styrofoam containers which you could use for
the transportation step. These can usually be made out of waste packaging
material--better than sending it to a landfill.
Yours,
Bill Tivol




From daemon Wed Jan 05 13:32:43 2000



From: Caroline Schooley :      schooley-at-mcn.org
Date: Wed, 5 Jan 2000 11:20:43 -0800 (PST)
Subject: Re: BIO-Bacterial Growth Medium

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} I know this is a little off the microscopy subject, but in the interest
} of encouraging scientific exploration in a young person. I hope you will
} indulge me.
}
} I'm looking for materials for my daughter's science project. She is
} testing the effectiveness of different mouthwashes in killing bacteria
} found in the mouth. Her experiment matrix requires 12 petri dishes with
} a bacterial growth medium. I can probably scrounge something to use as
} petri dishes, but the growth medium is a problem. I'm not even sure
} what it is properly called or what its makeup is. Is it something we
} can easily purchase locally, or even better is there a recipe for making
} a suitable substitute from ingredients found in the home?
}
} Any and all suggestions will be greatly appreciated.
}
Mike -

You're a supportive parent! I agree with you that purchasing prepared
microbiological groth media doesn't have as much learning potential as
starting from scratch, but it's undeniably easier. And probably cheaper.
How old is she? Since you obviously don't know any microtechnique, I worry
about the adequacy of her "experiment matrix". Some degree of sterile
technique is required, implying the use of an alcohol lamp-sterilized wire
loop.

If you want to cook your own, you'll need a pressure cooker and the
instructions available in Zook et al., "The Microcosmos guide to exploring
microbial space" (see the MICRO bibliography! URL below). I can send you
photocopied pages, but if this is a major project you may want the book.
Or you can buy prepared sterile plates (and the agarose that you'd need for
home cooking) from any large biological supply house, such as Carolina
Biological (800-334-5551). You may even have a medical supply source in
town.

Caroline


Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/PCI/pci.html





From daemon Wed Jan 05 15:32:24 2000



From: rfelten-at-Macdermid.com
Date: Wed, 5 Jan 2000 16:16:31 -0500
Subject: Looking for a Contract laboratory that does AFM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Rick Felten-at-MACDERMID
01/05/2000 04:16 PM
I have a couple of Ni/Au Samples that I need analyzed using AFM. The
conductive samples would need to be scanned over a 10 X 10 micron region.
Were are located in Waterbury Ct and the closer to us the better.

Any Help would be appreciated.

Ric





From daemon Wed Jan 05 16:02:18 2000



From: jmkrupp-at-cats.ucsc.edu (Jon Krupp)
Date: Wed, 5 Jan 2000 13:43:33 -0800
Subject: Image distortion correction?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


HI:

Is there a way to remove a moderate barrel distortion from an image? I have
images taken with a 17 mm wide angle lens that are slightly distorted and
wish to make some area measurements from them.

I have a picture of a grid of known size so it seems like there should be a
way to 'stretch' the picture into shape in something like Photoshop, I just
don't know where to look. Any help or ideas?

Thanks

Jonathan Krupp
Microscopy & Imaging Lab
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu





From daemon Wed Jan 05 16:12:20 2000



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Wed, 5 Jan 2000 12:04:59 -1000 (HST)
Subject: TEM, Sectioning, Need Ultracut belt

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi, all-

The drive belt on our Reichert (Leica) Ultracut E broke last night,
leaving a number of people in a state of panic. Leica does not have any
of these in the US, and the Vienna group is still on holiday.

I would appreciate and forever be indebted to anyone who could quickly
send me the appropriate belt, which I could either pay for (list $38.34,
plus shipping) or replace when ours come in.

Mahalo!
Tina

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************




From daemon Wed Jan 05 16:32:18 2000



From: Walck. Scott D. :      walck-at-ppg.com
Date: Wed, 5 Jan 2000 17:22:52 -0500
Subject: RE: Spectra in MSA/MSA Format How to Plot...

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Nestor,
The Gatan ELP option for MSA formatted files outputs the files to 5 columns
serial. There are other ASCII options, but they are not MSA format. I have
found that Noran does the same thing in our new system. It is not easy to
parse the data for a spreadsheet when it is in that format. I would have
been happy if they had done it with the two column option that is available
in the standard or if they simply used a single column. Once it is in that
format, it's easy to do in a spreadsheet. With multiple columns, I had to
go into the file with a word processor, take out the hard returns at the end
of each row, and then replace all of the commas with hard returns. Then I
could open them easily in the spreadsheet. That's how I got started with
the Basic program -simply to read them in and output them to a two column
text with the MSA format. Then I got carried away with VB.

-Scott



} -----Original Message-----
} From: Nestor J. Zaluzec [mailto:zaluzec-at-Sparc5.Microscopy.Com]
} Sent: Tuesday, January 04, 2000 8:14 PM
} To: microscopy-at-Sparc5.Microscopy.Com
} Subject: Spectra in MSA/MSA Format How to Plot...
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.} html
}
}
} --------------------------------------------------------------
} ---------.
}
}
}
} As one of the co-authors of the MSA/MAS File format we
} had a goal to make the format simple enough to be
} useable in even a simple spreadsheet program.
}
} A number of the major manufacturer's allow you to
} translate their data files into this format directly
} from their application programs. To use the data
} in a spreadsheet, simply specify dual column (x,y)
} format for the output file and
} then you can import the data files directly into
} a MS Excel spreadsheet, or even better a data
} graphing program like KaleidaGraph (which runs
} on both Mac's & PC's). Then plot to your hearts content.
}
}
}
} Nestor
} Your Friendly Neighborhood SysOp.
}
}
}



From daemon Wed Jan 05 17:42:07 2000



From: mykkb-at-juno.com
Date: Wed, 5 Jan 2000 18:28:19 -0500
Subject: Re:Bio Bacteria

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


There is a Bacterial Growth Medium Kit for grades 3-6 in the Carolina
K-6 Science Catalog ( I have the Fall 1998 Catalog). It contains 2 cans
of Beef Browth, 2 Boxes of Unflavored Gelatin, Multivitamins, 20 sterile
Petri Dishes and 10 sterile Cotton-Tipped Applicators. It recommends the
use of a pressure cooker or autoclave for "complete" sterility.
It costs $26.43 per kit (1998 price) Phone: 1-800-334-5551
Most of the items are available at the supermarket. The petri dishes and
sterile applicators are more difficult to get. A local college
microbiology department might give a hand or even some sterile Nutrient
Agar Plates.

Mike Baxter
Lehman College




From daemon Wed Jan 05 17:42:09 2000



From: bozzola-at-siu.edu (John J. Bozzola)
Date: Wed, 5 Jan 2000 17:37:02 -0600
Subject: Re: osmium's stability in a plastic container

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Donna,

Whenever I tried storing osmium solutions in plastics they always reacted
with the plastic causing it to blacken. This took place even in Teflon
altho at a much slower rate.

Why not use a glass that has been coated with plastic and rendered
breakproof. I see that many chemicals (like acids) come in such reagent
bottles.

Hint: maybe one of the EM vendors may know about this.

John



} We have always kept our osmium solutions in glass containers. However, we
} are in the process of evaluating our safety procedures and discussed the
} idea of increasing the safety of the transport of osmium from the
} refrigerator to the fume hood by putting the osmium solution in plastic
} containers. (If they are dropped, they would not break and cause the danger
} of a spill outside the hood.)
} Does anyone have experience with osmium stored in plastic? Any comments
} about this particular subject or any of your safety with osmium procedures
} are welcomed.

####################################################################
John J. Bozzola, Ph.D., Director
Micro-Imaging and Analysis Center
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
####################################################################





From daemon Wed Jan 05 18:12:04 2000



From: Earl Weltmer :      earlw-at-pacbell.net
Date: Wed, 05 Jan 2000 15:41:36 -0800
Subject: Mag. Calibration for JEOL 5800

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all,

The newer SEMs require software access to do some fundamental
adjustments: magnification, high voltage, crt brightness, etc.

I am attempting to calibrate the magnification on a JEOL 5800 SEM. I am
guessing this requires a sequence of keystrokes to access the computer
in "service" mode. Does anyone have the keystroke sequence or know of
the calibration procedure for the JEOL 5800?

Thank You,
Earl Weltmer




From daemon Thu Jan 06 07:35:21 2000



From: jim :      jim-at-proscitech.com.au
Date: Thu, 6 Jan 2000 10:51:49 +1000
Subject: RE: SEM-Refurbishing multi-standards mount

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We could quote on that and certainly, if it involved repolishing and re-coating
only this would be worthwhile. In this case the separated standards would need
to be identified, remounted and thoroughly polished. If much thickness is lost
during the polishing there may be a problem with other standards.
Certainly its possible but I expect that the cost will come close to our new
standard blocks, complete with micro-engraving. I suggest that you check it out
in our online.
Disclaimer: ProSciTech supplies WDS/EDS standards.

Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Wednesday, January 05, 2000 1:48 AM, Howard, Jean M. [SMTP:JMHoward-at-rmc.com]
wrote:
}
} Dear listers,
}
} I have a multi-element standards mount which over time has deteriorated and
} become contaminated with salts. Three of the standards have fallen out of
} the holder. The mount was made by C.M. Taylor Corp., Sunnyvale, Calif. in
} 1982, and it appears that this company no longer exists.
} I am interested in finding someone to re-polish the mount and replace the
} lost standards. Does anyone know of such a company/person?
}
} Thanks in advance,
}
} Jean M. Howard
} Reynolds Metals Company
} Materials Characterization-Electron Microscopy
} E-mail: jmhoward-at-rmc.com
} Office: 804.751.2554
}
}
}




From daemon Wed Jan 05 20:51:40 2000



From: Sara Miller :      saram-at-duke.edu
Date: Wed, 5 Jan 2000 21:29:07 -0500 (EST)
Subject: old Kinney evap instruc bk avail

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Some time ago I sent out a Kinney High Vacuum manual. There were 4
interested parties; one got the original; others, copies. I have now
found a 2nd one of these antiques, and would be happy to send it to the
highest bidder (i.e., free to the first to reply). The date in the front is
listed as June, 1961.

Sara E. Miller, Ph. D.
P. O. Box 3020
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-8735




From daemon Thu Jan 06 08:07:04 2000



From: Nestor J. Zaluzec :      zaluzec-at-Sparc5.Microscopy.Com
Date: Thu, 6 Jan 2000 07:59:23 -0600
Subject: RE: Spectra in MSA/MSA Format How to Plot...

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Scott etal...

Perhaps it is time to point out to some of the Manufacturers that they should
implement the MSA/MAS standard with an option to specify
the number of columns in the output file ( like the demo/test
copy does). That would be in the spirit of how the format
was originally designed so that "additional" programs code
would not have to be written. They generally listen to customers
so speak your mind!

In the mean time I'll look into also putting a version of the
demo translator on-line.


(Grinning Devilishly)

Nestor
Your Friendly Neighborhood SysOp

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America






From daemon Thu Jan 06 18:00:58 2000



From: Lou Solebello :      microls1297-at-mindspring.com
Date: Thursday, January 06, 2000 4:30 PM
Subject: SEM- service companies in Mexico

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Good Lord.......So this is what NAFTA brings us to.......Third world
companies undermining real science with a New Brand of Burger King products
and services? Care for an Enchilada while you wait for your SEM
images?.....$5.95 please.......

Just a cynical comment that is much closer to the truth than I care to think
about.
-----Original Message-----
} From: Paul Rennie (KIDDE) {Paul.Rennie-at-kidde-hq.com}
To: microscopy-at-Sparc5.Microscopy.Com {microscopy-at-Sparc5.Microscopy.Com}


Dear All,

Having just purchased a new SEM, our old instrument, a Cambridge S200 is
destined to be sent to one of our companies in Cuidad Victoria, Mexico
(Although I'm trying to persuade management that it's not worth it).
Although we will probably be installing the instrument ourselves, I would
welcome details of any service companies in Mexico. Additionally, who sells
consumables within Mexico, or is it best to purchase over the border in the
USA.

Thanking you in advance.

Paul Rennie

Kidde International Research
Mathisen Way
Colnbrook
Slough
Berks SL3 0HB
UK

Tel: 44 (0) 1753 683245
Fax: 44 (0) 1753 683810
http://www.kidde-int.com





From daemon Thu Jan 06 18:00:52 2000



From: Paul Rennie (KIDDE) :      Paul.Rennie-at-kidde-hq.com
Date: Thu, 6 Jan 2000 15:54:44 +0000
Subject: SEM- service companies in Mexico

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear All,

Having just purchased a new SEM, our old instrument, a Cambridge S200 is
destined to be sent to one of our companies in Cuidad Victoria, Mexico
(Although I'm trying to persuade management that it's not worth it).
Although we will probably be installing the instrument ourselves, I would
welcome details of any service companies in Mexico. Additionally, who sells
consumables within Mexico, or is it best to purchase over the border in the USA.

Thanking you in advance.

Paul Rennie

Kidde International Research
Mathisen Way
Colnbrook
Slough
Berks SL3 0HB
UK

Tel: 44 (0) 1753 683245
Fax: 44 (0) 1753 683810
http://www.kidde-int.com



From daemon Thu Jan 06 18:00:52 2000



From: :      UICPRINT-at-uic.edu
Date: Thu, 06 Jan 00 08:41:26 CST
Subject: FREE SCREENINGS IN JANUARY

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


For Sale:

JEOL 100B - Disassembled, great source for Parts . $2,000 Cash and Carry.

PHILLIPS 201C - Will be taken out of service this month. Excellent working
condition. Maintained on OEM Service Contracts since day one. Make an offer.

--
Ford M. Royer, MT(ASCP)
Analytical Instruments, Ltd
(Refurbished Histology, Cytology, & General Lab Equipment)
9921 13th Ave. N.
Minneapolis, MN 55441-5004
800-565-1895 phone
612-929-1895 fax
web site: http://www.aibltd.com




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Message-Id: {200001061637.KAA00322-at-Sparc5.Microscopy.Com}



The UIC Physicians Group at Central Station, 1550 South Indiana Avenue,
(312) 957-0049, is offering FREE SCREENINGS/TALKS in JANUARY:

"Stress Management"
Learn to manage the stress in your busy life.
All Fridays in January, 1:30-3:30 p.m.

"Bone Density Screening"
For the detection of osteoporosis
January 19--call for appointments

"Bowel and Bladder Management"
Tuesday, January 18, 9:30-11 a.m.
Friday, January 28, 9:30-11 a.m.

Special Feature
Are you overweight? Not sure? Find out your body mass index (BMI) and body
fat percentage. Also learn about UIC's new weight loss program and all
available options.
January 26, 9-11 a.m.




From: :      UICPRINT-at-uic.edu
Date: Thu, 06 Jan 00 08:41:26 CST
Subject: FREE SCREENINGS IN JANUARY

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



From daemon Thu Jan 06 18:00:52 2000



From: Robert Plano :      RPLANO-at-cea.com
Date: Thu, 6 Jan 2000 08:47:59 -0800
Subject: RE: Looking for a Contract laboratory that does AFM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html
id {CF52CAJP} ; Thu, 6 Jan 2000 08:48:07 -0800
Message-ID: {EAF569A980E4D21185380090271F40EB125F35-at-EXCHANGE}
{Microscopy-at-Sparc5.Microscopy.Com} ,
"',rfelten-at-Macdermid.com'"
{,rfelten-at-Macdermid.com}


Rick,

While we are not too close to Connecticut (California to be exact), we have
a great deal of experience in analyzing all types of samples with the AFM
and overnight delivery services can make turnaround times very short. What
information are you looking for from the samples? Please give me a call at
650-962-8767 or respond to this email so we can help you out.

-Rob

Robert J. Plano
Staff Analyst, SPM Services
Surface Science Labs
(650)962-8767, ext. 742
http://www.cea.com
http://www.surface-science.com


} -----Original Message-----
} From: "rfelten-at-Macdermid.com"-at-Sparc5.Microscopy.Com
} [mailto:"rfelten-at-Macdermid.com"-at-Sparc5.Microscopy.Com]
} Sent: Wednesday, January 05, 2000 1:17 PM
} To: Microscopy-at-Sparc5.Microscopy.Com
} Subject: Looking for a Contract laboratory that does AFM
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListser} ver/FAQ.html
}
}
} --------------------------------------------------------------
} ---------.
}
}
}
}
} Rick Felten-at-MACDERMID
} 01/05/2000 04:16 PM
} I have a couple of Ni/Au Samples that I need analyzed using AFM. The
} conductive samples would need to be scanned over a 10 X 10
} micron region.
} Were are located in Waterbury Ct and the closer to us the better.
}
} Any Help would be appreciated.
}
} Ric
}
}
}



From daemon Thu Jan 06 18:00:56 2000



From: rgriffin-at-eng.uab.edu
Date: Thu, 6 Jan 2000 12:11:25 -0600
Subject: 8 inch 10 megabyte bernoulli flexible disk cartridges

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have about 45 USED 8 inch 10 megabyte bernoulli flexible disk cartridges
that I will ship to the first person that emails me at rgriffin-at-eng.uab.edu.
Due to shipping charges, I will only ship to a continental U.S. address.

We have gotten rid of our computer that uses these disks and I thought
someone out there that still needed them might appreciate them (since you
can't buy them anymore!).

Robin Griffin
Materials and Mechanical Engineering
The University of Alabama at Birmingham



From daemon Thu Jan 06 18:00:57 2000



From: Corazon Bucana :      bucana-at-audumla.mdacc.tmc.edu
Date: Thu, 06 Jan 2000 12:35:35 -0600
Subject: subscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


*******************************************************
Corazon D. Bucana, Ph.D.
Department of Cancer Biology
U.T. M.D. Anderson Cancer Center
1515 Holcombe Blvd. Box 173
Houston, Texas 77030
Phone: (713) 792-8106
FAX: (713) 792-8747
Email:bucana-at-audumla.mdacc.tmc.edu
FAX: (713) 792-8747




From daemon Thu Jan 06 18:00:59 2000



From: Barbara Foster :      mme-at-map.com
Date: Thu, 06 Jan 2000 15:24:14 -0500
Subject: Re: SEM- service companies in Mexico

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Paul,

Contact Gordon Grau Scientific (407-282-5749)

While they are in Florida, they deal extensively with Latin America. As
for either Dr. Neddy Fookes or Dr. Barry Fookes... tell them I sent you.

Best regards,
Barbara Foster
Consortium President
Microscopy/Microscopy Education ...Educating microscopists for greater
productivity.

125 Paridon Street Suite 102 Springfield, MA 01118
PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com
Visit our web site {http://www.MME-Microscopy.com/education}
******************************************************
MME is America's first national consortium providing
customized on-site workshops in all areas of
microscopy, sample preparation, and image analysis.


At 03:54 PM 1/6/00 +0000, Paul Rennie (KIDDE) wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Thu Jan 06 18:01:01 2000



From: Ladd Research :      ladres-at-worldnet.att.net
Date: Thu, 06 Jan 2000 16:11:22 -0500
Subject: Re: osmium's stability in a plastic container

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Osmium vapors can penetrate and blacken plastics. The long term effects
are uncertain. If you can find a Teflon bottle this might work.

Probably best to stay with glass bottles with Teflon colsures and do not
hand carry the solution (i.e. use a lab cart for transport). Hope this
is some help.

Charles Duvic, Ph.D.
Chief Chemist

--

LADD RESEARCH
131 Dorset Lane
Williston, VT 05495

TEL 1-800-451-3406 (US) or 1-802-878-6711 (anywhere)
FAX 1-802-878-8074
e-mail ladres-at-worldnet.att.net
web site http://www.ladd.cc



From daemon Thu Jan 06 18:01:02 2000



From: jjerome-at-wfubmc.edu (Jay Jerome)
Date: Thu, 06 Jan 2000 16:38:45 -0500
Subject: Survey of Graduate Students

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


This was sent to the MSA Education Committee. It seems to be a worthwhile
endeavor, so I am passing it along to those on the listserver.
Jay Jerome

Adam Fagen wrote:

} Dear Microscopy Community:
}
} The National Association of Graduate-Professional Students (NAGPS) has
} recently received a grant from the Alfred P. Sloan Foundation to conduct a
} survey of doctoral students on their graduate school experiences. The
} survey will be completed on the Web {http://survey.nagps.org/} by current
} and recent doctoral students from January - May 2000, and the results made
} publicly available on the Web on a department specific basis in September.
}
} This effort is a follow-up to a more limited survey which occurred this
} past spring, which was aimed at science and engineering doctoral students.
} The aggregate results from that survey are available at
} {http://www.phds.org/survey/results/} .
}
} The survey we are conducting is unique in at least two important ways: it
} collects information on a department-specific basis, not only averaged
} over entire institutions or disciplines (though discipline-level results
} will also be available). So it will be possible to look at, for instance,
} responses from individual biology programs, or to rank history departments
} based on faculty mentoring. And it makes this data publicly available on
} the Internet in Fall 2000. So we'll be opening the door about the
} situation in individual departments for wider viewing by graduate
} students, prospective students, faculty, administrators, etc.
}
} The survey is based upon best practices and covers issues in a number of
} areas, including information for prospective students, curriculum breadth
} and flexibility, career guidance and placement services, faculty
} mentoring, time to degree, department climate, teaching, professionalism,
} and overall satisfaction. In other words, the sort of best practices and
} concerns outside of the reputation. The NAGPS survey itself will run from
} January 18-May 1, 2000, and will be available on the Web at
} {http://survey.nagps.org/} (which already has a number of resources).
}
} For this survey to be useful, it is vital that we reach as many current
} and recent doctoral students (anyone who has been enrolled for at least
} one semester in the past five years) as possible. We are hoping that we
} can encourage a significant percentage of students to respond so that the
} results will represent a broad range of experiences and a realistic
} picture of department and institutional practices.
}
} In order to realize this level of participation, getting the word out is
} obviously very important. One of the publicity strategies we're employing
} is trying to reach current and recent doctoral students through the major
} professional societies and organizations, such as the MSA. (Other
} strategies include messages to relevant e-mail listservs, coverage in
} campus and national media, and working through graduate student
} organizations, department chairs and graduate deans, other organizations,
} etc.)
}
} Since the MSA reaches so many current and recent graduate students, it is
} certainly one of the organizations of prime importance for getting the
} word out. We have thought of a few strategies for spreading the word
} among your membership (and would welcome additional ideas and
} suggestions): (1) distribution on e-mail listservs that reach a high
} number of graduate students and recent graduates, those who have left
} their programs, etc.; (2) notice in newsletters and other publications
} that current and former students might see; and (3) publicity at meetings
} and conferences. We are happy to provide whatever resources and materials
} that would facilitate distribution (e.g., flyers, letters, posters, etc.).
} We would certainly appreciate any insight you have on publicity within
} (and outside of) the MSA.
}
} As a bit of background on our organization, NAGPS represents nearly
} 900,000 graduate and professional students on 150 member campuses and is
} dedicated to improving the quality of graduate and professional student
} life and education by actively promoting the interests and welfare of
} graduate- and professional-degree-seeking students.
}
} Of course, I'd be happy to answer any questions or provide any more info.
} Thanks for your assistance in this important effort!
}
} --Adam Fagen
}
} Adam Fagen, Chair
} Ad Hoc Committee on Faculty-Student Relations
} National Association of Graduate-Professional Students (NAGPS)
}
} NAGPS Web: http://www.nagps.org/
} The National Doctoral Program Survey: http://survey.nagps.org/
}
} Adam Fagen \ afagen-at-fas.harvard.edu
} Molecular Biology/Education / http://www.fas.harvard.edu/~afagen/
} Harvard University GSAS \ http://mazur-www.harvard.edu/

--
Jay
----------------------------------------------
- AKA: W. Gray Jerome, Ph.D. -
- Department of Pathology -
- Wake Forest University School of Medicine -
- Winston-Salem, NC 27157-1092 -
- Ph: 336-716-4972, 336-716-2675 -
- Fax: 336-716-6174 -
- E-mail: jjerome-at-wfubmc.edu -
----------------------------------------------





From daemon Fri Jan 07 07:08:42 2000



From: Rick Harris :      raharris-at-ucdavis.edu
Date: Thu, 06 Jan 2000 16:46:43 -0800
Subject: Image montage software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Can anyone recommend image montaging software? That is, software that
assists you in compositing several images into one larger image. I am
familiar with the offerings from GATAN for their Digital Micrograph and
QuickStitch from Enroute Software. Also, can anyone offer an evaluation of
the QuickStitch software?

TIA


Rick A. Harris, Director
Microscopy and Imaging Facility
Section of Molecular and Cellular Biology
1241 Life Sciences Addition
University of California
Davis, CA
530 752 2914
530 754 7536 fax
http://katie.ucdavis.edu
raharris-at-ucdavis.edu



From daemon Fri Jan 07 07:08:49 2000



From: jim :      jim-at-proscitech.com.au
Date: Fri, 7 Jan 2000 15:11:32 +1000
Subject: RE: osmium's stability in a plastic container

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Plastic is oxidized and so part of the osmium is exhausted in the vials. Even
when frozen osmium diffuses through plastic containers. So you may save a rare
breakage, but you are certain to have osmium in your refrigerator. It's just a
bad idea to pack osmium in plastic. You could use a secondary container, be it
plastic or glass, to increase overall safety.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Thursday, January 06, 2000 3:08 AM, Donna Wagahoff
[SMTP:DWAGAHOFF-at-siumed.edu] wrote:
}
} We have always kept our osmium solutions in glass containers. However, we
} are in the process of evaluating our safety procedures and discussed the
} idea of increasing the safety of the transport of osmium from the
} refrigerator to the fume hood by putting the osmium solution in plastic
} containers. (If they are dropped, they would not break and cause the danger
} of a spill outside the hood.)
} Does anyone have experience with osmium stored in plastic? Any comments
} about this particular subject or any of your safety with osmium procedures
} are welcomed.
} Thanks.
}
} Donna Wagahoff
} SIU School of Medicine
} PO Box 19627
} Springfield, IL 62794-9627
} 217-782-0898
} fax217-524-3227




From daemon Fri Jan 07 07:08:49 2000



From: Chris Jeffree :      cjeffree-at-srv0.bio.ed.ac.uk
Date: Fri, 7 Jan 2000 10:24:22 +0000
Subject: Re: osmium's stability in a plastic container

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I would not recommend storing osmium in ordinary laboratory
plastics containers used on their own. Osmium penetrates
polyolefin plastics (PE, PP) to some depth, and reacts with them,
so containers made from these plastics or of polystyrene or
polycarbonate with polyolefin closures cannot be recommended.
Mostly, osmium is supplied in some protective packaging, but if
you want to improve security still further I would consider placing
the glass ampoules inside a polyethylene or polypropylene tube.
That would give considerable shock-protection, and if the ampoule
did break would protect against leakage, but only for the short
period required to get it to a fume cupboard. The same principle
can be applied to osmium solutions prepared in glass bottles -
enclosure in an outer polyethylene bottle will give shock-protection
and temporary containment. It will also indicate by its blackening
how much leakage is occurring from your supposedly closed glass
container. Speaking of which, we have a rule that osmium solutions
are NEVER stored in the laboratory refrigerator. Why? because
even when greatest care is taken, osmium blackening of the fridge
walls and other objects in the fridge takes place, indicating that
vapour is escaping into the fridge atmosphere. If you must store
osmium at low temperatures, I recommend you consider installing
a compact refrigerator in your fume hood or in a fan-ventilated
cupboard.

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} We have always kept our osmium solutions in glass containers. However, we
} are in the process of evaluating our safety procedures and discussed the
} idea of increasing the safety of the transport of osmium from the
} refrigerator to the fume hood by putting the osmium solution in plastic
} containers. (If they are dropped, they would not break and cause the danger
} of a spill outside the hood.)
} Does anyone have experience with osmium stored in plastic? Any comments
} about this particular subject or any of your safety with osmium procedures
} are welcomed.
} Thanks.
}
} Donna Wagahoff
} SIU School of Medicine
} PO Box 19627
} Springfield, IL 62794-9627
} 217-782-0898
} fax217-524-3227
}


=====================================================================
DR CHRIS JEFFREE
BIOSEM - BIOLOGICAL SCIENCES EM FACILITY
UNIVERSITY OF EDINBURGH
Daniel Rutherford Building
King's Buildings, Mayfield Road
EDINBURGH, EH9 3JH, Scotland, UK
Tel. #44 131 650 5345
FAX. #44 131 650 6563
Mobile 0410 585 401
email c.jeffree-at-ed.ac.uk
SEM / TEM bookings sem-at-ed.ac.uk
=====================================================================



From daemon Fri Jan 07 07:38:57 2000



From: Petra Wahlbring :      wahlbrin-at-crpgl.lu
Date: Fri, 07 Jan 2000 14:16:32 +0100
Subject: Mat: Cutting of small semiconductor specimen?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



A happy New Year to everybody!

I just solved (with the help of some of you) my last problem with the
carbon extraction replicas but the next specimen causing difficulties is on
my desk...

I got a small semiconducor specimen with a layered structure that should be
investigated. If I say small I mean it: it has a shape like a cube with a
side length of about 250um. And now comes the real difficulty: We need to
cut this one precious specimen into several slices in order to allow other
investigations with other analytical methods. In fact we have five
specimens to try with, but they are not completely identical to the one we
have finally to investigate.

We are equipped with everything we need to do a TEM preparation of bulk
materials and also of interfaces as long as the specimens are large enough
(saw, disk cutter, dimpler, ion etcher, mounting tools, ...) but I don't
see how I could be successful with such a small specimen.

Any ideas?

Petra


--------------------------------------------------------------
Dr. Petra Wahlbring
Centre de Recherche Public - Gabriel Lippmann
Laboratoire d'Analyse des Materiaux (LAM)
162a, av. de la Faiencerie L-1511 Luxembourg
tel. +352-466644-402 fax +352-466644-400
e-mail: petra.wahlbring-at-crpgl.lu
Visit our WWW site! http://www.crpgl.lu/~wahlbrin



From daemon Fri Jan 07 07:58:55 2000



From: Petra Wahlbring :      wahlbrin-at-crpgl.lu
Date: Fri, 07 Jan 2000 14:16:32 +0100
Subject: Mat: Cutting of small semiconductor specimen?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



A happy New Year to everybody!

I just solved (with the help of some of you) my last problem with the
carbon extraction replicas but the next specimen causing difficulties is on
my desk...

I got a small semiconducor specimen with a layered structure that should be
investigated. If I say small I mean it: it has a shape like a cube with a
side length of about 250um. And now comes the real difficulty: We need to
cut this one precious specimen into several slices in order to allow other
investigations with other analytical methods. In fact we have five
specimens to try with, but they are not completely identical to the one we
have finally to investigate.

We are equipped with everything we need to do a TEM preparation of bulk
materials and also of interfaces as long as the specimens are large enough
(saw, disk cutter, dimpler, ion etcher, mounting tools, ...) but I don't
see how I could be successful with such a small specimen.

Any ideas?

Petra


--------------------------------------------------------------
Dr. Petra Wahlbring
Centre de Recherche Public - Gabriel Lippmann
Laboratoire d'Analyse des Materiaux (LAM)
162a, av. de la Faiencerie L-1511 Luxembourg
tel. +352-466644-402 fax +352-466644-400
e-mail: petra.wahlbring-at-crpgl.lu
Visit our WWW site! http://www.crpgl.lu/~wahlbrin



From daemon Fri Jan 07 18:08:50 2000



From: Eric Windsor :      Eric.Windsor-at-nist.gov
Date: Fri, 07 Jan 2000 09:40:08 -0500
Subject: Re: Mat: Cadmium Zinc Telluride thin foils

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dorrance,

I think I can make several suggestions and point you toward several references
that may help.

First, can you or have you tried dipping your ion milled (to perforation)
samples into your bromine/methanol etch as the final step in your procedure?
Yu et al (J.of Elec. Micro.Tech./18/315-324/1991) dipped their perforated
samples in 0.5% Br2/Ch3OH for 1or 2 seconds followed by 20 seconds in HF/HCL of
volume ratio 1:1. This technique worked well for them.

As you may know, reducing the energy during the final step(s) of ion milling
can reduce the severity and/or the number of artifacts produced. If you have
access to a mill that allows milling in the 100eV to 1keV range you may want to
try this as the final step in your milling procedure.

The following techniques have also been used to prepare type II-VI compound
semiconductors:

(1) Reactive ion milling. This can be done 2 different ways. In one
method
Iodine gas is used instead of Argon. The iodine gas is ionized to form I+,
which is then used for milling (Cullis and Chew/ MRS symposium proceedings/
115/ 3-14/1988). In the second method, Iodine gas is introduced into the
“Atmosphere” of the milling chamber during Ar+ milling (Pecz and Barna
/Vacuum/45/1-3/1994). The authors have applied these techniques to type II-VI
compound semiconductors of CdTe, ZnS, and ZnSe in addition to other compound
semiconductors. CAUTION must be used when introducing iodine gas into your ion
mill. The gas is very corrosive and may damage mill parts and vacuum systems.
I recommend that you check with the manufacturer of your ion mill before
proceeding.
(2) Chemomechanical polishing. Sabinina and Gutakovsky
(Ultramicroscopy/45/411-415/1992) eliminated the need for ion milling
completely by using this technique. They prepare samples of HgCdTe using a
bromine-methanol etchant in a technique that is very similar to dimpling using
padded tools.


Are you familiar with the Small Angle Cleavage Technique (SACT)? This
technique may or may not work for your materials. I am not aware of any
references for preparing II-VI materials using SACT but that certainly does not
imply that it can’t be done. I have used this technique to prepare samples of
thin films on silicon substrates. It is quick and relatively easy to learn.
The technique was developed by John McCaffrey and a good step by step procedure
is given by Walck and McCaffrey (MRS symposium proceedings/480/149-171/1997).

The references listed above are meant to be a starting point and are by no
means a complete listing.

Hope this helps.

E. Windsor

Eric Windsor
Physical Scientist
NIST
100 Bureau Dr. Stop 8371
Gaithersburg, MD 20899-8371
(301) 975-3930
fax: (301) 417-1321
eric.windsor-at-nist.gov

Dorrance McClean originally wrote:

Hi,
I've been working on thin foils of CZT and I'm looking for some suggestions
to help eliminate artifacts created by ion milling. So far I have polished
side one and etched (to remove polishing damage) with bromine-methanol and
then dimpled side two, ending with 0.1um alumina. I have been successful
obtaining a final sample thickness of about 8 to 10 microns. However, when
I put the samples into the PIPs (Gatan) to complete the thinning process I'm
seeing beam damage. I was hoping that someone might have a different
approach or suggestion that would eliminate the beam damage.
Thanks for your help.
Dorrance




From daemon Fri Jan 07 18:08:48 2000



From: David Rohde :      drohde-at-zuul.noran.com
Date: Fri, 7 Jan 2000 08:41:45 -0600
Subject: Image montage software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Soft Imaging Systems has a program called analySIS that has a montage module
called MIA. Have a look at their web site at: www.soft-imaging.de

David Rohde
NORAN Instruments

-----Original Message-----
} From: Rick Harris [mailto:raharris-at-ucdavis.edu]
Sent: Thursday, January 06, 2000 6:47 PM
To: microscopy-at-sparc5.microscopy.com


Can anyone recommend image montaging software? That is, software that
assists you in compositing several images into one larger image. I am
familiar with the offerings from GATAN for their Digital Micrograph and
QuickStitch from Enroute Software. Also, can anyone offer an evaluation of
the QuickStitch software?

TIA


Rick A. Harris, Director
Microscopy and Imaging Facility
Section of Molecular and Cellular Biology
1241 Life Sciences Addition
University of California
Davis, CA
530 752 2914
530 754 7536 fax
http://katie.ucdavis.edu
raharris-at-ucdavis.edu





From daemon Fri Jan 07 18:08:49 2000



From: mcannon-at-bio.umass.edu (Maura Cannon)
Date: Fri, 7 Jan 2000 09:53:47 -0500
Subject: in situ hyb

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Subscribers,
I would be interested in hearing from anyone who has experience in in situ
hybridization to mRNA. I am working with immature embryos of Arabidopsis
and would like to recieve replies and comments to the following questions:

1. For embedding, Paraffin is mostly used.
Why not use LR-White (London Resin)?
or BMM (Butylmethylmethacrylate)?
or another acrylic material?
These hydrophilic materials should be easier to remove, or is it necessary
to remove them for mRNA exposure?
If it is necessary, what is best for removing them? Perhaps acetone?

2. About the probe itself, obviously RNA is best. Has anyone tried DNA
oligos (~20 nucleotides) for less abundant mRNAs?

3. Has anyone used tailed oligos?

4. Is DIG significantly better to use than biotin labelled probes?

Many thanks to anyone who is prepared to take the time to help reveal the
unknowns of plant embryogenesis.
Maura
____________________
Dr. Maura C. Cannon
Dept. Biochemistry & Molecular Biology
Lederle Graduate Research Center
University of Massachusetts
Amherst, MA 01003






From daemon Fri Jan 07 18:08:49 2000



From: Walck. Scott D. :      walck-at-ppg.com
Date: Fri, 7 Jan 2000 09:54:45 -0500
Subject: RE: Cutting of small semiconductor specimen?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ron Anderson has a technique that might do the trick for you. He took an
ultrasonic drill and made a small cavity in a piece of Si at the surface.
The drill was solid spherical end. He then epoxied his small sample into
the hole. You might want to mount it on another piece of Si first and then
put it in the hole. He then used the Tripod Polishing technique to examine
it. The benefit is that he has the Si to gauge the thickness of the sample.
I though that it was pretty slick.

Your other option and the one most easy to do if you have access to an
instrument is to have the sample FIB'd.

-Scott


Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)


"The opinions expressed are those of Scott D. Walck and not of PPG
Industries, Inc. nor of any PPG-associated companies."
--


} -----Original Message-----
} From: Petra Wahlbring [mailto:wahlbrin-at-crpgl.lu]
} Sent: Friday, January 07, 2000 8:17 AM
} To: microscopy-at-Sparc5.Microscopy.Com
} Subject: Mat: Cutting of small semiconductor specimen?
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.} html
}
}
} --------------------------------------------------------------
} ---------.
}
}
}
} A happy New Year to everybody!
}
} I just solved (with the help of some of you) my last problem with the
} carbon extraction replicas but the next specimen causing
} difficulties is on
} my desk...
}
} I got a small semiconducor specimen with a layered structure
} that should be
} investigated. If I say small I mean it: it has a shape like a
} cube with a
} side length of about 250um. And now comes the real
} difficulty: We need to
} cut this one precious specimen into several slices in order
} to allow other
} investigations with other analytical methods. In fact we have five
} specimens to try with, but they are not completely identical
} to the one we
} have finally to investigate.
}
} We are equipped with everything we need to do a TEM
} preparation of bulk
} materials and also of interfaces as long as the specimens are
} large enough
} (saw, disk cutter, dimpler, ion etcher, mounting tools, ...)
} but I don't
} see how I could be successful with such a small specimen.
}
} Any ideas?
}
} Petra
}
}
} --------------------------------------------------------------
} Dr. Petra Wahlbring
} Centre de Recherche Public - Gabriel Lippmann
} Laboratoire d'Analyse des Materiaux (LAM)
} 162a, av. de la Faiencerie L-1511 Luxembourg
} tel. +352-466644-402 fax +352-466644-400
} e-mail: petra.wahlbring-at-crpgl.lu
} Visit our WWW site! http://www.crpgl.lu/~wahlbrin
}



From daemon Fri Jan 07 18:08:53 2000



From: rlvaughn-at-unmc.edu
Date: Fri, 7 Jan 2000 09:30:03 -0600
Subject: Re: Osmium in plastic bottle

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Every plastic container we have seen turns black and some seals break down.
Our current method is similar to Bill Trivol's. We use a 25ml Pyrex media
bottle with the orange plastic lid (it does not seem affected). This is
placed in a spare metal can (that the ampules of osmium crystals were
shipped in) with paper padding. This new bottle (with parafilm around
cap) also solved our osmium fumes in the refrig. problem. Everything is
small enoug to transport from refrig. to hood.

Rick Vaughn




From daemon Fri Jan 07 18:08:52 2000



From: JIM ROMANOW :      bsgphy3-at-uconnvm.uconn.edu
Date: Fri, 7 Jan 2000 11:22:11 -0500
Subject: Re: osmium's stability in a plastic container

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


ON 1/7/00 DR CHRIS JEFFREE WROTE:

Speaking of which, we have a rule that osmium solutions
are NEVER stored in the laboratory refrigerator. Why? because
even when greatest care is taken, osmium blackening of the fridge
walls and other objects in the fridge takes place, indicating that
vapour is escaping into the fridge atmosphere. If you must store
osmium at low temperatures, I recommend you consider installing
a compact refrigerator in your fume hood or in a fan-ventilated
cupboard.

With all due respect Chris for over 20 years we have used our lab
refrigerator to store Osmium with no blackening of the plastic walls. A
25ml 4% solution of Osmium is put into a 60 ml glass stopper bottle with
the stopper tightly wrapped in parafilm. Next, that bottle is placed in a
waxed 6" long x 3" diameter screw capped cardboard mailing tube. We prepare
the waxed tubes by pouring hot paraffin inside and rotated quickly and
evenly until the entire surface area is well coated. We also coat the
inside of the metal screw cap but not the outside of the tube. Mailing
tubes are available from the local shipping supply house and our waxed
containers have lasted decades.
Regards
Jim

James S. Romanow
The University of Connecticut
Physiology and Neurobiology Department
Electron Microscopy Facility
U-2131
Storrs, CT 06269-2131
bsgphy3-at-uconnvm.uconn.edu
860 486-2914 voice
860 486-1936 fax





From daemon Fri Jan 07 18:08:53 2000



From: Greg Erdos :      gwe-at-biotech.ufl.edu
Date: Fri, 07 Jan 2000 12:48:42 -0500
Subject: Re: Osmium in plastic bottle

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have been using teflon bottles for many yeas with success. We
got them from Fisher Sci

At 09:30 AM 1/7/00 -0600, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Gregory W. Erdos, Ph.D. Ph. 352-392-1295
Assistant Director, Biotechnology Program
PO Box 110580 Fax:
352-846-0251
University of Florida
Gainesville, FL 32611





From daemon Fri Jan 07 18:08:57 2000



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Fri, 07 Jan 2000 11:57:20 -0800
Subject: Re: osmium's stability in a plastic container

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Donna Wagahoff wrote:

} We have always kept our osmium solutions in glass containers. However, we
} are in the process of evaluating our safety procedures and discussed the
} idea of increasing the safety of the transport of osmium from the
} refrigerator to the fume hood by putting the osmium solution in plastic
} containers. (If they are dropped, they would not break and cause the danger
} of a spill outside the hood.)
} Does anyone have experience with osmium stored in plastic? Any comments
} about this particular subject or any of your safety with osmium procedures
} are welcomed.

Dear Donna,

In my point of view polypropylene and polyethylene are compatible with
osmium tetroxide solutions. Only problem - to hold that nasty stuff inside
the vial. Some plastics may partially be penetrable by osmium tetroxide.

I had some limited experience to store aqueous osmium tetroxide solutions
in the polypropylene (?) NUNC 2 or 5 ml cryo-vials. I aliquot solution
into that vials and store it at -40oC. For extra protection I stored
cryo-vials in the 15 ml cell-culture tubes. Cryo-vial's seal may penetrate
by osmium tetroxide vapors a little bit but second tube provides complete
protection. 2-4% aqueous osmium tetroxide solutions (no buffer, please)
are stable for at least 1-2 month in the dark at -20 - 40oC in that plastic
containers.

Good luck.

Sergey.

_________________________________

Sergey Ryazantsev Ph. D.
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu
http://www.bol.ucla.edu/~sryazant





From daemon Fri Jan 07 18:08:57 2000



From: Bill Chissoe :      wchiss-at-ou.edu
Date: Fri, 07 Jan 2000 14:21:38 -0600
Subject: SEM of biofilms

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear List,

There is a researcher at our Med. School who wants to do SEM on biofilms

on ear biopsies. He says the biopsies will be approximately 1mmX1mm. I
proposed a standard glut.-osmium fix, Et-oh dehydration, CPD, mount,
sput coat protocol. Is this sufficient? Do we need to affix the biopsies

to a substrate first? I would appreciate any suggestions. If anybody has
reprints that contain a good protocol, I would certainly appreciate
getting a copy. My address is in the footer and my FAX is 405-325-7619.
Thanks for your help.

Bill Chissoe


--
=============================================================
Bill Chissoe III
Electron Microscopist
University of Oklahoma
770 Van Vleet Oval
Norman, Ok. 73019
E-mail: wchiss-at-ou.edu Ph. (405)325-4391
=============================================================





From daemon Fri Jan 07 18:09:01 2000



From: John Chandler :      chandler-at-lamar.ColoState.EDU
Date: Fri, 7 Jan 2000 15:55:56 -0700
Subject: Vac Evap: Special needs request

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


A user of our facility wishes to do some special vacuum evaporation
processes. He is looking for ceramic coated tungsten boats for the
purpose, but has not been able to locate a source. Any help would be
appreciated.

Replies offline would be welcome. Please reply to the list only if you
have generally useful information.

Thanks,

John Chandler
Colorado State University
chandler-at-lamar.colostate.edu





From daemon Fri Jan 07 18:09:01 2000



From: White, Woody N :      Woody.N.White-at-mcdermott.com
Date: Fri, 7 Jan 2000 17:23:00 -0600
Subject: BSE imaging artifact - Help!

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello All,

For years, I have been using my diode GW BSE system with high beam currents
and
high gain (contrast) to yield electron channeling contrast on polished
specimens.

FWIW: Contrary to some reports I have read, lower kV works better than
higher.
Since it is imperative to use high beam currents at the required resolution,
I
wonder if difficulty in achieving sufficient current at lower potentials may
have been influencing opinions.

I have also been a very good customer for GW Electronics. When the detector
is
new, I generally have no problem, but as it ages, the same operating
conditions
will produce artifacts. A new detector every year or so is a bit expensive,
but
fixes the problem.

I now have a new detector (and SEM :) which produces this artifact and am
now
trying to find the reason.

The artifact can be described as video brightness overshoot from black to
white
when the black is strongly saturated. It occurs when the (very high gain)
BSE
signal goes from saturated black to some median gray. A low-Z inclusion or
deep
pore in the field of view will produce this artifact. When the beam leaves
the
inclusion/pore, it overshoots to white then settles back to the appropriate
level. The effect is a black pore with a white "comet tail" pointing in the
sweep direction. This problem does not manifest itself when using less
extreme
operating conditions.

Any suggestions about how to avoid the artifact?

TIA for your comments and help.

Woody White
McDermott Technology, Inc.



From daemon Fri Jan 07 18:09:01 2000



From: White, Woody N :      Woody.N.White-at-mcdermott.com
Date: Fri, 7 Jan 2000 17:41:00 -0600
Subject: More on BSE artifact

Contents Retrieved from Microscopy Listserver Archives
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PS...

I certainly cannot exclude the possibility that the detector is not the
direct
cause. The amount of signal gain (contrast) is not calibrated (nor have I
paid
much attention to the position of the adjustment) . If the detector simply
loses some sensitivity, I would make up the difference in amplifier gain and
not
realize I was not at exactly the same operating conditions. ...Thus, the
artifact could be amplifier gain related rather than simply a detector
problem.

Woody



From daemon Sat Jan 08 07:54:27 2000



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Sat, 08 Jan 2000 00:16:19 -0500
Subject: Shipping to Mexico

Contents Retrieved from Microscopy Listserver Archives
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-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Paul Rennie wrote:
===============================================
Having just purchased a new SEM, our old instrument, a Cambridge S200 is
destined to be sent to one of our companies in Cuidad Victoria, Mexico
(Although I'm trying to persuade management that it's not worth it).
Although we will probably be installing the instrument ourselves, I would
welcome details of any service companies in Mexico. Additionally, who sells
consumables within Mexico, or is it best to purchase over the border in the
USA.
================================================
With regard to the method of purchasing consumables from within Mexico, the
situation is entirely analogous to that that exists in the UK vis a vis
purchasing items from the USA directly or via a distributor in the UK. It
is a matter of institutional policy and also, personal preference.

The main manufacturers of consumables all have local distributors in Mexico
and those firms can be found on the websites of those respective firms. On
the other hand, with the implimentation of NAFTA, making a shipment to a
point in Mexico from the USA is not really all that different from making a
shipment to some other point in the USA. So again it is a matter of
institutional policy and personal preference.

Chuck


============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.spi.cc
########################
============================================



From daemon Sat Jan 08 12:07:30 2000



From: Lugosi1936-at-aol.com
Date: Sat, 8 Jan 2000 12:41:36 EST
Subject: LM Vickers Microscope M1500974C

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I have been trying for some time to find any information about a Vickers
Instrument Company microscope. I had never heard of the Company but the scope
looked interesting. I have searched hundreds of websites, posted messages on
newsgroups and also on Microscopy, UK. So far I've had only two responses.
The sum total of what I have learned is that the company was in York and that
they closed in 1989. Cooke, Troughton and Simms were taken over by Vickers

The rather lengthy description of the scope follows:

Head: Binocular configuration, adjustable for interpupillary distance and
dioptric differences. Interpupillary adjustment range, 50 to 72mm.
Eyepieces(2): 10X, DIN standard. Objectives(4): Vickers microplan,
Planachromatic objectives. Include 5X (0.15 N.A.), 10X (0.25 N.A.), 20X (0.50
N.A. spring loaded), 40X (0.65 N.A. spring-loaded), DIN standard objectives.
All are parfocal, parcentered, and coated to resist reflection. Stage:
Precision-machined mechanical stage with oversized, low-position, coaxial
control knobs. Chemical-resistant finish with glass insert. This stage is
exceptionally smooth in movement with a 3.25 x 2.75-inch range of motion.
Focus Control: Low-position, coaxial, coarse/fine controls. . Condenser: Abbe
condenser, fitted with an iris diaphragm. Illumination: Diascopic lower
illumination features variable-intensity quartz halogen lamp ( 12V, 100 watt
) with metered solid-state control as well as field iris, condenser and
centering adjustments. Episcopic illumination (30-watt lamp) is also of
variable intensity via an independent control on front of the microscope
base. Upper illuminator housing fitted with iris and condenser controls. As
shown in the photos, the microscope can be separated from the 100-watt
illuminator base Finish chemical-resistant paint with "hammertone" finish.

I have included some pictures that I have posted to help with the
identification:

{A HREF="http://members.aol.com/lugosi1936/vic1a.jpg"} http://members.aol.com/l
ugosi1936/vic1a.jpg {/A}
{A HREF="http://members.aol.com/lugosi1936/vic2.jpg"} http://members.aol.com/lu
gosi1936/vic2.jpg {/A}
{A HREF="http://members.aol.com/lugosi1936/vic3.jpg"} http://members.aol.com/lu
gosi1936/vic3.jpg {/A}

I am trying to find any information about the company and about the scope
itself. I understand that this is rather difficult (impossible?) because of
the practice of the company not to use serial numbers. I would especially
like to be able to aquire a copy of the owners manual and a copy of the
Vickers catalog.

When I began to attempt to collect information about the Vickers I never
guessed that I would run into a blank wall. If you could assist me in any way
at all it would be greatly appreciated.

Best regards,
Dana



From daemon Sun Jan 09 10:49:57 2000



From: Melany H. Chapin :      mchapin-at-ntbg.org
Date: Sat, 8 Jan 2000 13:42:27 -1000
Subject: Histochemical test for lipids/oils in plant tissue

Contents Retrieved from Microscopy Listserver Archives
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I am looking for a simple method to indicate the presence of lipids /oils
in plant tissue. Specifically palm fruit tissues. Ideally, if there is a
procedure that I can used on fruits fixed in FAA that would be the best. I
have tried Sudan Black B without success. Thank you in advance for your
ideas or suggestions. Please reply via email to : mchapin-at-ntbg.org

________________________________________________________________________

Melany H. Chapin Herbarium (PTBG)
Curator & Plant Records Manager ph: 808-332-7324 ext. 133
National Tropical Botanical Garden (NTBG) fax: 808-332-9765

3530 Papalina Road email: mchapin-at-ntbg.org
Kalaheo, Kauai, Hawaii 96741 www.ntbg.org
USA
___________________________________________________________________________





From daemon Sun Jan 09 10:50:14 2000



From: Campbell36-at-aol.com
Date: Sun, 9 Jan 2000 10:45:02 EST
Subject: Re: Vac Evap: Special needs request

Contents Retrieved from Microscopy Listserver Archives
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The item you are looking for should be available from R.D. Matis. The
following contact information is from www.vacuum.org. There are several
vendor listed in the subsection for filaments if you care to browse. I have
no financial interest in R.D. Matis.

R.D. Mathis Company
2840 Gundry Avenue Long Beach, CA 90806
Phone: 562-426-7049
FAX: 562-595-0907

Description

Specializes in the manufacture of hi-vacuum evaporation sources. We offer a
comprehensive selection of tungsten, molybdenum and tantalum sources as well
as custom fabrication to meet your specific needs. Display will be a variety
of evaporation sources along with one of our "LV Series" low voltage high
current power supplies and our "GP 100" inert gas purifier to compliment your
evaporation process.

Product Categories
Filaments



Good luck

Jim Campbell

James Campbell
36 Van Drive
Bordentown, NJ 08505
Tel: 609-298-9206
Fax: 609-278-6969
e-mail campbell36-at-aol.com



In a message dated 1/7/00 9:56:09 PM Eastern Standard Time,
chandler-at-lamar.ColoState.EDU writes:

} Subj: Vac Evap: Special needs request
} Date: 1/7/00 9:56:09 PM Eastern Standard Time
} From: chandler-at-lamar.ColoState.EDU (John Chandler)
} To: Microscopy-at-Sparc5.Microscopy.Com
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} A user of our facility wishes to do some special vacuum evaporation
} processes. He is looking for ceramic coated tungsten boats for the
} purpose, but has not been able to locate a source. Any help would be
} appreciated.
}
} Replies offline would be welcome. Please reply to the list only if you
} have generally useful information.
}
} Thanks,
}
} John Chandler
} Colorado State University
} chandler-at-lamar.colostate.edu



James Campbell
36 Van Drive
Bordentown, NJ 08505
Tel: 609-298-9206
Fax: 609-278-6969
e-mail campbell36-at-aol.com



From daemon Sun Jan 09 19:10:36 2000



From: Michael L. Boucher :      mboucher-at-isd.net
Date: Sun, 9 Jan 2000 13:58:37 -0600
Subject: Vickers

Contents Retrieved from Microscopy Listserver Archives
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Dana:
This may not help, but I have a book I purchased about 1970 that was
published by Vickers entitled:
The Polarizing Microscope by A.F. Hallimond.
It is an excellent work and has many pictures of the Vickers line of
polarizing scopes and accessories from the late 60s. They did buy out Cooke,
Troughton and Simms and marketed a number of innovative and competitively
priced (cheaper than Leitz and Zeiss) scopes. Dr Hallimond was one of their
consultants on design. I am sure you can find the book in a large University
Geology department library ( I hope). Or try interlibrary loan. It is still
an excellent and definitive reference for polarizing light microscopes and
their use.

Michael L. Boucher Sr.
mboucher-at-isd.net
http://www.isd.net/mboucher




From daemon Sun Jan 09 19:10:39 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Sun, 09 Jan 2000 15:50:09 -0800
Subject: Low Z contrast quantitative analysis

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I am trying to quantify the alloy amount of Al/Si. The standard
alloy ratio is 1-3 wt % Si/Al. However, with Z of each only 1 unit
apart, and low Z at that, EDAX and AES cannot detect the Si.
My next attempt is to try dynamic SIMS and then time of flight SIMS.

The specimen is a microcircuit die. I am analyzing the bonding pad
metalization.

Has anyone done this sort of thing before and had success? If so,
how did you do it?

gary g.




From daemon Sun Jan 09 19:10:39 2000



From: Barbara Foster :      mme-at-map.com
Date: Sun, 09 Jan 2000 18:53:50 -0500
Subject: Re: LM Vickers Microscope M1500974C

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dana,

Vickers was a well known British manufacturer of microscopes. I have had
the privilege of using some of their more interesting measuring equipment.
Several contacts come to mind, most of them from the UK: Clive Cowan, Micro
Instruments in Oxford (UK) 199-388-9616 and Gerard Turner, who is a curator
in microscopy at the Museum of Science at Oxford University, Dr. Savile
Bradbury, retired from Oxford (but could probably be reached by letter
there; also, if you are interested, I can probably get a more recent
address), and Dr. Cecile Fox at Molecular Histology in Gaithersburg
(301-216-1564). Cecile put together major microscopy exhibits for both the
Smithsonian and the Am. Mus. of Natural History in the mid- 1980's. Please
give my regards to them (Gerard may only remember me as an RMS student from
the distant past) and best of luck on your search.

Best regards,
Barbara Foster
Consortium President
Microscopy/Microscopy Education ...Educating microscopists for greater
productivity.

125 Paridon Street Suite 102 Springfield, MA 01118
PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com
Visit our web site {http://www.MME-Microscopy.com/education}
******************************************************
MME is America's first national consortium providing
customized on-site workshops in all areas of
microscopy, sample preparation, and image analysis.




At 12:41 PM 1/8/00 EST, Lugosi1936-at-aol.com"-at-Sparc5.Microscopy.Com wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Mon Jan 10 08:06:03 2000



From: jim :      jim-at-proscitech.com.au
Date: Mon, 10 Jan 2000 13:39:27 +1000
Subject: RE: Sputter coating

Contents Retrieved from Microscopy Listserver Archives
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Do you have an evaporator in the lab? Simultaneous evaporation of Pt (wire) and
carbon gives a very fine and permanent coating.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Thursday, January 06, 2000 2:15 AM, Tindall, Randy D.
[SMTP:TindallR-at-missouri.edu] wrote:
}
}
} Hi,
}
} Due to some recent high-resolution requirements in our lab, I find myself
} having to go back to Sputter Coating 101 (after years of just putting
} specimens in the coater and turning it on without a second thought!). We
} find ourselves in need of very thin coatings with as little structure as
} possible, in order to image samples down in the nanometer range.
}
} We have a chromium coater, but often need to revisit samples days or weeks
} after the initial coating. The oxidation problem rears its ugly head. We
} intend to purchase a platinum target, but don't yet have one, so we're
} experimenting with our venerable Au/Pd coater.
}
} My questions are:
}
} 1) I seem to remember a string on this listserver suggesting that lower
} deposition currents yield finer coating structure. Is this right? Does a
} low deposition current for a longer time yield a finer coating than a higher
} current for a shorter time? (I'm running some tests to check this, but
} would be very interested in others' experiences, too.)
}
} 2) Deposition current can be controlled by the initial current setting
} (i.e., the knob on the machine) and by the argon flow through the chamber.
} Is there any difference in the coating when adjusting the deposition current
} by either of these two ways?
}
} 3) Charts I have seen indicate that deposition current is directly
} proportional to coating rate. Is the same true for coating time? I.e., is a
} one minute coating twice as thick as a 30 sec. coating? It would seem so
} intuitively, but you know what they say about the word "assume".
}
} My apologies if these are very basic questions, but, like I said, back to SC
} 101!
}
} I'll be happy to summarize the responses for anyone who is interested.
}
} Thanks!
}
} Randy
}
} Randy Tindall
} EM Specialist
} Electron Microscopy Core Facility
} W122 Veterinary Medicine Bldg.
} University of Missouri
} Columbia, MO 65211
} (573)882-8304
} tindallr-at-missouri.edu
} http://www.biotech.missouri.edu/emc/
}




From daemon Mon Jan 10 17:49:35 2000



From: rschoonh-at-sph.unc.edu
Date: Mon, 10 Jan 2000 09:54:45 -0500 (Eastern Standard Time)
Subject: Re: LM Vickers Microscope M1500974C

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dana,

I'm not 100% sure but I thought that the Microscience Division of Bio-Rad bought
Vickers some years ago. You might want to give them a shot.

best regards,
Bob
Robert Schoonhoven
Laboratory of Molecular Carcinogenesis and Mutagenesis
Dept. of Environmental Sciences and Engineering
University of North Carolina
CB#7400
Chapel Hill, NC 27599
Phone
office 919-966-6343
Lab 919-966-6140
Fax 919-966-6123

Don't go around saying the world owes you a living; the world owes you
nothing; it was here first.
Mark Twain [Samuel Langhornne Clemens] (1835-1910)

-- Begin original message --
} -----------------------------------------------------------------------.
}
}
}
} I have been trying for some time to find any information about a Vickers
} Instrument Company microscope. I had never heard of the Company but the scope
} looked interesting. I have searched hundreds of websites, posted messages on
} newsgroups and also on Microscopy, UK. So far I've had only two responses.
} The sum total of what I have learned is that the company was in York and that
} they closed in 1989. Cooke, Troughton and Simms were taken over by Vickers
}
} The rather lengthy description of the scope follows:
}
} Head: Binocular configuration, adjustable for interpupillary distance and
} dioptric differences. Interpupillary adjustment range, 50 to 72mm.
} Eyepieces(2): 10X, DIN standard. Objectives(4): Vickers microplan,
} Planachromatic objectives. Include 5X (0.15 N.A.), 10X (0.25 N.A.), 20X (0.50
} N.A. spring loaded), 40X (0.65 N.A. spring-loaded), DIN standard objectives.
} All are parfocal, parcentered, and coated to resist reflection. Stage:
} Precision-machined mechanical stage with oversized, low-position, coaxial
} control knobs. Chemical-resistant finish with glass insert. This stage is
} exceptionally smooth in movement with a 3.25 x 2.75-inch range of motion.
} Focus Control: Low-position, coaxial, coarse/fine controls. . Condenser: Abbe
} condenser, fitted with an iris diaphragm. Illumination: Diascopic lower
} illumination features variable-intensity quartz halogen lamp ( 12V, 100 watt
} ) with metered solid-state control as well as field iris, condenser and
} centering adjustments. Episcopic illumination (30-watt lamp) is also of
} variable intensity via an independent control on front of the microscope
} base. Upper illuminator housing fitted with iris and condenser controls. As
} shown in the photos, the microscope can be separated from the 100-watt
} illuminator base Finish chemical-resistant paint with "hammertone" finish.
}
} I have included some pictures that I have posted to help with the
} identification:
}
} {A HREF="http://members.aol.com/lugosi1936/vic1a.jpg"} http://members.aol.com/l
} ugosi1936/vic1a.jpg {/A}
} {A HREF="http://members.aol.com/lugosi1936/vic2.jpg"} http://members.aol.com/lu
} gosi1936/vic2.jpg {/A}
} {A HREF="http://members.aol.com/lugosi1936/vic3.jpg"} http://members.aol.com/lu
} gosi1936/vic3.jpg {/A}
}
} I am trying to find any information about the company and about the scope
} itself. I understand that this is rather difficult (impossible?) because of
} the practice of the company not to use serial numbers. I would especially
} like to be able to aquire a copy of the owners manual and a copy of the
} Vickers catalog.
}
} When I began to attempt to collect information about the Vickers I never
} guessed that I would run into a blank wall. If you could assist me in any way
} at all it would be greatly appreciated.
}
} Best regards,
} Dana
}
}

-- End original message --




From daemon Mon Jan 10 17:49:37 2000



From: Michael Reiner :      michael.reiner-at-Smail.Uni-Koeln.de
Date: Mon, 10 Jan 2000 16:23:07 +0100
Subject: EM: Shelf life of LR-Gold

Contents Retrieved from Microscopy Listserver Archives
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Dear members of the list,

first, I would like to wish you all the best for the new year.
Now my question:
Does anyone know the shelf-life/usability of LR-Gold stored in a fridge?
Is it that delicate as LR-White (Meanwhile I don«t use LR-W older than
half a year). My bottle which was not opened many times, could be
roundabout three years old.

Thanks a lot,
Michael

Michael Reiner
Department of Anatomy I
University of Cologne
Germany
michael.reiner-at-smail.uni-koeln.de




From daemon Mon Jan 10 17:49:37 2000



From: carol williams :      cswill-at-acd.tusk.edu
Date: Mon, 10 Jan 2000 09:23:30 -0600
Subject: gamma correction - image analysis

Contents Retrieved from Microscopy Listserver Archives
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Please send me (if possible) a brief explanation of gamma correction in
image analysis and a good reference source (book, papers, etc) to get
the details... Thanks



From daemon Mon Jan 10 17:49:40 2000



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Mon, 10 Jan 2000 08:48:07 -0800
Subject: Re: SEM: Sputter coating

Contents Retrieved from Microscopy Listserver Archives
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Dear Randy,
There was a good series of articles in the Scanning Electron Microscopy,
1980, volume I (pp. 143--218), that examined a number of thin-film
deposition methods and measured the films for feature size, mostly using
TEM. They found that lower kV made for finer films, using Mo, W or Ta made a
more featureless coating, Pt was a little finer than Au/Pd and that lowering
the temperature of the substrate also made the films finer.
In my own experience I found that, on smoother specimens, even a two second
coating would sometimes be enough to stop charging. Try very short times and
turn the specimen and coat again for a very short time, if the first one
isn't enough. I used 700V, if your coater can change voltage.
If you can find the Scanning Electron Microscopy articles, they have other
good suggestions.
At 10:15 AM 1/5/00 -0600, you wrote:

} Hi,
}
} Due to some recent high-resolution requirements in our lab, I find myself
} having to go back to Sputter Coating 101 (after years of just putting
} specimens in the coater and turning it on without a second thought!). We
} find ourselves in need of very thin coatings with as little structure as
} possible, in order to image samples down in the nanometer range.
}
} We have a chromium coater, but often need to revisit samples days or weeks
} after the initial coating. The oxidation problem rears its ugly head. We
} intend to purchase a platinum target, but don't yet have one, so we're
} experimenting with our venerable Au/Pd coater.
}
} My questions are:
}
} 1) I seem to remember a string on this listserver suggesting that lower
} deposition currents yield finer coating structure. Is this right? Does a
} low deposition current for a longer time yield a finer coating than a higher
} current for a shorter time? (I'm running some tests to check this, but
} would be very interested in others' experiences, too.)
}
} 2) Deposition current can be controlled by the initial current setting
} (i.e., the knob on the machine) and by the argon flow through the chamber.
} Is there any difference in the coating when adjusting the deposition current
} by either of these two ways?
}
} 3) Charts I have seen indicate that deposition current is directly
} proportional to coating rate. Is the same true for coating time? I.e., is a
} one minute coating twice as thick as a 30 sec. coating? It would seem so
} intuitively, but you know what they say about the word "assume".
}
} My apologies if these are very basic questions, but, like I said, back to SC
} 101!
}
} I'll be happy to summarize the responses for anyone who is interested.
}
} Thanks!
}
} Randy
}
Best of luck,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca




From daemon Mon Jan 10 17:49:43 2000



From: Joanne Crudele :      Joanne.Crudele-at-unilever.com
Date: Mon, 10 Jan 2000 12:01:46 -0600
Subject: CryoTEM or SEM

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Microscopy-at-MSA.Microscopy.Com

Is anyone willing to do some contract cryoSEM or TEM in the Chicagoland area? I
have a primary emulsion with particle size less than 1.0 micrometers. 2 samples.
Please contact me via the server. Joanne Crudele-Unilever-Rolling Meadows Il.
Give a price estimate per sample.



From daemon Mon Jan 10 17:49:42 2000



From: White, Woody N :      Woody.N.White-at-mcdermott.com
Date: 1/8/00 7:11 PM
Subject: Re: More on BSE artifact

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John,

You are corrrect in that the time constant of diode BSE detectors is
relatively
slow. Unfortunatly, I am already sweeping about as slowly as possible to
minimize the effect and help the signal to noise ratio.

Woody
____________________Reply Separator____________________

Woody,
Is the problem independent of scan speed? Slower scan speeds
often overcome distortion using solid state BEI detectors.


At 05:41 PM 1/7/00 -0600, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Mon Jan 10 08:06:03 2000



From: IKSM :      IKSM-at-aphy.iphy.ac.cn
Date: Mon, 10 Jan 2000 19:3:24 +0800
Subject: IKSM: latest news -- call for paper

Contents Retrieved from Microscopy Listserver Archives
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id AA30574; Mon, 10 Jan 2000 19:01:49 +0800
Message-Id: {200001101101.AA30574-at-aphy.iphy.ac.cn}


Hi, Microscopist,

International Kunming Symposium on Microscopy (IKSM)
will be held on July 2-5, 2000, in Kunming, one of the
most attractive tourist destinations in China.

Call-for-paper circular and pre-registration form for
International Kunming Symposium on Microscopy (IKSM)
are available on request by email. For more information,
pls visit http://www.iphy.ac.cn/microsc/IKSM.html .

Happy a new year 2000.

Regards,
IKSM secretariat
IKSM-at-aphy.iphy.ac.cn




From daemon Mon Jan 10 17:49:48 2000



From: rfelten-at-Macdermid.com
Date: Mon, 10 Jan 2000 16:20:40 -0500
Subject: Re: Image montage software

Contents Retrieved from Microscopy Listserver Archives
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Rick Felten-at-MACDERMID
01/10/2000 04:20 PM
Whenever I wanted to merge images I used corel draw 8. I inserted the
images where I wanted them and exported the entire image as a tiff. Seem
to work ok. Not sure if it is the most efficient way. In fac, it is the
only thing that I liked about corel draw over MS publisher.

Ric





From daemon Mon Jan 10 17:49:48 2000



From: MICHAEL DELANNOY :      delannoy-at-mail.jhmi.edu
Date: Mon, 10 Jan 2000 16:43:55 -0500 (EST)
Subject: Spotty artifacts

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To the Board,
We have a problem with spotty,black,amorphous artifacts on our
SEM samples visible at 200X and up. The problem seems to arise only
with one sample type, epoxy resin casts made with amine-blush resistant
hardener. We use professional dental impression molds,a two part process, base
and catalyst to make the flexible mold (President, polyvinylsiloxane). Then
Araldite 506 epoxy with HY 356 hardener is poured and cured at room temp
overnight. Sputter coating glass slides with gold palladium does not
seem to cause the artifact, but another question, is gold palladium more
susceptible to oxide formation than pure gold, and do oxides look like
the above described artifact. Also, etching the sample does not help.
Any suggesstions will be greatly appreciated. Thanks.

Mike D.




From daemon Mon Jan 10 17:49:50 2000



From: Sara Miller :      saram-at-duke.edu
Date: Mon, 10 Jan 2000 18:13:14 -0500 (EST)
Subject: Re: Osmium in plastic bottle

Contents Retrieved from Microscopy Listserver Archives
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We store Os in a glass bottle with Parafilm around the top, inside a
larger plastic jar with a corn oil-soaked paper towel taped to the
inside of the lid, and then the outer container is Parafilmed around the
top. No black frig. (Change the oil-soaked towel periodically.)

Sara E. Miller, Ph. D.
P. O. Box 3020
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-8735




From daemon Mon Jan 10 17:49:50 2000



From: Dusevich, Vladimir :      DusevichV-at-umkc.edu
Date: Mon, 10 Jan 2000 17:25:02 -0600
Subject: RE: Sputter coating

Contents Retrieved from Microscopy Listserver Archives
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I use Denton spatter coater with Au/Pd target for magnifications
up to 100,000 without any problems. I can observe collagen striations
and other fine details and do not see coating structure.
Parameters for coating: current 15 ma, time 5-10 sec. Some charging can occur
for samples with "multilayer" surface, but it is tough case
for almost any coating anyway. I have also magnetron Cr coater, but
do not use it often because it's much more time consuming.

Vladimir


Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
3127 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy

} -----Original Message-----
} From: Tindall, Randy D. [mailto:TindallR-at-missouri.edu]
} Sent: Wednesday, January 05, 2000 10:15 AM
} To: 'microscopy-at-sparc5.microscopy.com'
} Subject: SEM: Sputter coating
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} ---------.
}
}
} Hi,
}
} Due to some recent high-resolution requirements in our lab, I
} find myself
} having to go back to Sputter Coating 101 (after years of just putting
} specimens in the coater and turning it on without a second
} thought!). We
} find ourselves in need of very thin coatings with as little
} structure as
} possible, in order to image samples down in the nanometer range.
}
} We have a chromium coater, but often need to revisit samples
} days or weeks
} after the initial coating. The oxidation problem rears its
} ugly head. We
} intend to purchase a platinum target, but don't yet have one, so we're
} experimenting with our venerable Au/Pd coater.
}
} My questions are:
}
} 1) I seem to remember a string on this listserver suggesting
} that lower
} deposition currents yield finer coating structure. Is this
} right? Does a
} low deposition current for a longer time yield a finer
} coating than a higher
} current for a shorter time? (I'm running some tests to check
} this, but
} would be very interested in others' experiences, too.)
}
} 2) Deposition current can be controlled by the initial current setting
} (i.e., the knob on the machine) and by the argon flow through
} the chamber.
} Is there any difference in the coating when adjusting the
} deposition current
} by either of these two ways?
}
} 3) Charts I have seen indicate that deposition current is directly
} proportional to coating rate. Is the same true for coating
} time? I.e., is a
} one minute coating twice as thick as a 30 sec. coating? It
} would seem so
} intuitively, but you know what they say about the word "assume".
}
} My apologies if these are very basic questions, but, like I
} said, back to SC
} 101!
}
} I'll be happy to summarize the responses for anyone who is interested.
}
} Thanks!
}
} Randy
}
} Randy Tindall
} EM Specialist
} Electron Microscopy Core Facility
} W122 Veterinary Medicine Bldg.
} University of Missouri
} Columbia, MO 65211
} (573)882-8304
} tindallr-at-missouri.edu
} http://www.biotech.missouri.edu/emc/
}
}



From daemon Mon Jan 10 18:48:38 2000



From: Nestor J. Zaluzec :      zaluzec-at-Sparc5.Microscopy.Com
Date: Mon, 10 Jan 2000 18:41:35 -0600
Subject: Osmium Vapor- A Safety Question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Posted at author's request to remain anonymous....


} To: zaluzec-at-Sparc5.Microscopy.Com
} Date: Mon, 10 Jan 2000 01:20:59 +0530
} Subject: Nestor, would you post this for me?
}
} Osmium Vapor- A Safety Question
}
} The last time I mentioned a safety concern about the rumor of an possible
} "explosive" warning about some Ruthenium compound , I got "Battered" by
} certain vendors(for quite a while too I might add..certainly didn't help
} my previously supportive, pro-vendor position-since I used to be one!) .
} I was warned and pestered frequently and asked to retract any suggestion
} of such a possibility, but I refused. So here we go again....
}
} On approximately January 6, 2000 I noticed a request under the
} title:
}
} : Donna Wagahoff {DWAGAHOFF-at-siumed.edu}
}
} "....Does anyone have experience with osmium stored in
} plastic?..."
}
} and the only responses on the list server were:
}
} " How about putting the glass containers inside plastic containers?
} There are also padded and styrofoam containers which you could use for
} the transportation step."
}
} and
}
} ......" I store this glass bottle inside an aluminum-foiled plastic
} container in my fume hood at room
} temp. The clear plastic of this outer container gets translucent black
} within weeks no matter how carefully I seal the glass bottle".......
}
} ( I'm sure these were very fine suggestions..but..)
}
} This concerns me for several reasons:
}
} 1. I believe there is a considerably more dangerous situation here than
} many of us may realize, or..will discuss. We must check, and we may find
} that Osmium and Ruthenium compounds(and others?) may penetrate all
} plastics and we may only see this at a macroscopic level long after the
} heavy metal compounds or there derivatives have penetrated many layers
} and possible contaminated at some lower and less visible level( but
} undetermined danger level that may be a health risk) a much larger area.
} Many of us may have seen the effect in EM refrigerators as the interior
} blackens over a long period of storage of Osmium.
}
} 2. There doesn't seem to be an acceptable safe level of these compounds
} or known affects, but probably fixation or contamination and loss of
} function at some level occurs(microscopic?).
}
} 3.Also venting these vapors through hoods should be scrubbed or
} absorbed(?), reduced,etc.( "I'm no chemist") and the level of the output
} probably should be carefully and technically monitored.
}
} 4. The safety level must be obvious to those who receive bottles of
} solutions in sealed glass(or Pirex?) and the factories that manufactor
} them.(What glass type materials are safe?)
}
} Does anyone know of the level of danger(or safety) or know of some good
} research sites or periodicals that have dealt with these issues? Any
} personal experience, or are we all afraid of retribution and retaliation?
} The MSDS's seem a little less than thorough to me. I'm sure I'm again not
} alone here in this concern and only wish to continue this difficult and
} frequently dangerous craft(EM) with more care for myself , my associates
} and my community.
}
} Anonymous (chicken....)
} (...and Vendors and manufacturers are our backbone and our support!
} Thank you all!)
}
}
}
}





From daemon Mon Jan 10 18:49:56 2000



From: ELMA Cortinas :      ECORTI-at-childmed.dallas.tx.us
Date: Mon, 10 Jan 2000 18:44:54 -0600
Subject: Negative film scanner

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does anyone have an idea of a good negative film scanner to use for
TEM negatives? I am trying to eliminate the darkroom printing
process. These are the stipulations:
1. Must be able to scan 3 1/4 x 4 inch negatives. (Kodak 4489
film)
2. Must be compatible to a PC.
3. Need to know what software is needed.
4. Must be priced under $3,000.00
5. Must provided the best quality resolution for diagnostic
results.

Thanks in advance!





From daemon Mon Jan 10 18:59:46 2000



From: Mike Mizell :      MMizell-at-CompuServe.COM
Date: Mon, 10 Jan 2000 18:47:22 -0600
Subject: Re: SEM: Sputter Coating

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Randy;

It is our experience, at South Bay Technology, that Cr films deposited by
ion beam sputtering remain conductive for a short time. The amount of time
depends on the film thickness and sample surface topography. Because Cr
deposition requires a water vapor free environment, usually not possible in
a sputter coater, you may have more success with Ir target. Under 200Kx,
ion beam deposited Ir (better than Pt since Ir films minimize beam damage)
does not display any artifacts (grains cannot be seen) or specimen damage.
We recommend using Cr (or Ti, W or Ta) only when looking at mag } 200Kx.

An Ir target in your sputter coater may work well and an Ir target in your
"Cr coater" may solve the oxidation problem more effectively.

Some advantages of Ion Beam Sputtering:
- Controlled thickness on angstrom level since the average deposition rates
are 10A/min
- Precise thickness measurements reported by quartz thickness monitor as
result of low energy sputterant energy striking crystal
- No damage or artifacts as a result of 30ev sputterant energy
- Any material can be deposited although Cr is suggested for } 200Kx mags,
Ir for {200Kx
- C like metal films are amorphous. C ddoes not display grains or create
damage
- X-ray production from 10A films is lost in the noise
- Image improvement results from increased signal to noise as well as
conductivity

Disclosure: South Bay Technology is the manufacturer of the IBS/E Ion Beam
Sputtering and Etching System and therefore has a vested interested
interest in promoting it use.

Regards, Mike Mizell

*************************************************************************
Michael K. Mizell Tele: 949-492-2600
VP Sales & Marketing Fax: 949-492-1499
South Bay Technology
1120 Via Callejon mizell-at-southbaytech.com
San Clemente, Ca 92673 USA
**************************************************************************
South Bay Technology is an American manufacturer of precision
sample preparation equipment and supplies for metallography
crystallography and electron microscopy.

} } } } } } } Please visit us at http://www.southbaytech.com { { { { { { { {





From daemon Mon Jan 10 21:45:09 2000



From: Pauline C. Yu :      splene-at-pw.usda.gov
Date: Mon, 10 Jan 2000 16:57:55 -0800 (PST)
Subject: Microtomes available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The following items are available to anyone who wants to pay for
shipping or pickup:

American Optical Microtome Knife Sharpener with Grit and Glass Planes

American Optical 820 Rotary Microtome with extra knives

Labline/Hooker (Sledge-type) Plant Microtome

Pauline Yu
pyu-at-pw.usda.gov
510-559-5938
Microscopist Technician
USDA-ARS-WRRC




From daemon Mon Jan 10 21:45:09 2000



From: sumka :      sumka-at-vsnl.com
Date: Mon, 10 Jan 2000 19:06:50 -0600
Subject: what is a nomarski disc

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I would like to know what is a nomarski disc. What it is used for? Is there
any difference between this and the routine phase contrast condenser we use
in light microscopy. Regards, M. Angou SUMKA SONS INSTRUMENTATION1137-B,
THADAGAM ROAD,R S PURAM, COIMBATOREINDIA - 641002 FAX: 
91-422-473227TEL:91-422-474378 URL:www.sumka.com





From daemon Tue Jan 11 18:01:06 2000



From: Michael Reiner :      michael.reiner-at-Smail.Uni-Koeln.de
Date: Tue, 11 Jan 2000 17:25:46 +0100
Subject: EM: Shelf life of LR-Gold

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear members of the list,

first, I would like to wish you all the best for the new year.
Now my question:
Does anyone know the shelf-life/usability of LR-Gold stored in a fridge?

Is it that delicate as LR-White (Meanwhile I don«t use LR-W older than
half a year). My bottle which was not opened many times, could be
roundabout three years old.

Thanks a lot,
Michael

Michael Reiner
Department of Anatomy I
University of Cologne
Germany
michael.reiner-at-smail.uni-koeln.de





From daemon Tue Jan 11 18:01:08 2000



From: JIM ROMANOW :      bsgphy3-at-uconnvm.uconn.edu
Date: Tue, 11 Jan 2000 11:52:55 -0500
Subject: Negative film scanner

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We scan our 4489 negs with a UMAX PowerLook III regularly on a Mac G3. The
UMAX is compatable with PCs too, cost about 1100 dollars last summer with
the transparancy adaptor and has 1200x2400 dpi actual resolution. We had to
modify the 4x5" transparancy holder (easy) to fit the smaller EM negs. UMAX
tech help could improve but the scanner works well. Stand alone or
PhotoShop plug-in software. Connect via SCSI.

Other units to check out are the Agfa DuoScan T2500 and Microtek ScanMaker 5.

The major electronic trade show meetings are soon so all of the new
'improved' equipment will be ordered and available for summer/fall 2000.
The remaining stock of last year's discontinued models will be discount
priced by Spring.
Good Luck



} Date: Mon, 10 Jan 2000 18:44:54 -0600
} To: Microscopy-at-Sparc5.Microscopy.Com
} From: "ELMA Cortinas" {ECORTI-at-childmed.dallas.tx.us}
} Subject: Negative film scanner
}

}
} Does anyone have an idea of a good negative film scanner to use for
} TEM negatives? I am trying to eliminate the darkroom printing
} process. These are the stipulations:
} 1. Must be able to scan 3 1/4 x 4 inch negatives. (Kodak 4489
} film)
} 2. Must be compatible to a PC.
} 3. Need to know what software is needed.
} 4. Must be priced under $3,000.00
} 5. Must provided the best quality resolution for diagnostic
} results.
}
} Thanks in advance!
}

James S. Romanow
The University of Connecticut
Physiology and Neurobiology Department
Electron Microscopy Facility
U-2131
Storrs, CT 06269-2131
bsgphy3-at-uconnvm.uconn.edu
860 486-2914 voice
860 486-1936 fax





From daemon Tue Jan 11 18:01:11 2000



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Tue, 11 Jan 2000 13:09:22 -0600
Subject: Negative film scanner

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Elma,

We are using an Epson Expression 800 and have been very happy with it. We
routinely scan in at 600 dpi, which has been more than enough for
publication quality, in our experience. The unit is capable of higher
resolutions than that. I don't remember what we paid, but it was
considerably less than $3000. It came with a transparency adapter and all
necessary software, including SilverFast, text recognition software,
calibration software, etc. Ours works through Adobe Photoshop's Import
functions, but may be usable in other programs, too.

Best wishes,
Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine Bldg.
University of Missouri
Columbia, MO 65211
(573)882-8304
tindallr-at-missouri.edu
http://www.biotech.missouri.edu/emc/


-----Original Message-----
} From: ELMA Cortinas [mailto:ECORTI-at-childmed.dallas.tx.us]
Sent: Monday, January 10, 2000 6:45 PM
To: Microscopy-at-Sparc5.Microscopy.Com


Does anyone have an idea of a good negative film scanner to use for
TEM negatives? I am trying to eliminate the darkroom printing
process. These are the stipulations:
1. Must be able to scan 3 1/4 x 4 inch negatives. (Kodak 4489
film)
2. Must be compatible to a PC.
3. Need to know what software is needed.
4. Must be priced under $3,000.00
5. Must provided the best quality resolution for diagnostic
results.

Thanks in advance!





From daemon Tue Jan 11 18:01:13 2000



From: Kenneth A. Taylor :      taylor-at-bio.fsu.edu
Date: Tue, 11 Jan 2000 15:39:52 -0500
Subject: Postdoctoral Position Available Immediately

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


{fontfamily} {param} Times_New_Roman {/param} {smaller} Postdoctoral
position available immediately to study the 3-D structure of insect
flight muscle. The research project, recently renewed for a 4 year
period, involves several experimental and theoretical approaches to
studying crossbridge structure in different states. Approaches include
electron microscope tomography, alignment and classification of 3-D
crossbridge structures (see recent publication (Winkler & Taylor,
Ultramicroscopy 77:141-152 (1999) for details) and fitting of atomic
coordinates of actin and myosin S1 into the envelope of the 3-D images.
Emphasis is on the study of quick-frozen, contracting muscle,
freeze-substituted for thin section electron microscopy and 3-D image
reconstruction. We use stretch activated muscle as well as an
isometrically contracting state dubbed stretch activation. Specimens
are mechanically monitored right up the point of freezing to facilitate
the interpretation of the structures in terms of muscle force and
stiffness. Parallel X-ray diffraction experiments make for thoroughly
characterized specimens. Please see recent publication (Taylor et al.,
Cell 99:421-431 (1999)) for current status of this project. This
project is a collaboration with Michael and Mary Reedy (Duke Univ. Med.
Center), Yale Goldman and Clara Franzini-Armstrong (U. Penn. Med.
School) and Richard Tregear (MRC, Cambridge, UK). Successful applicants
can work on any of several aspects of the problem of identifying
structural features and relating them to the generation of tension in
muscle. The project has evolved to the point that most of the effort
needs to be put on classification and averaging, model building, model
refinement and interpretation of X-ray data. An individual with
experience in either image reconstruction or protein structure and
function who is interested in gaining further experience in the
interpretation and correlation of diverse experimental data on a
topical biophysical problem would be ideal for this position. The
experimental emphasis of correlating high resolution structures with
lower resolution EM data is expected to become a growth area for
structural biology. Our laboratory is equipped with Silicon Graphics
workstations, one of which is dedicated to this position, a cluster of
3 DEC Alpha compute servers, a Perkin-Elmer PDS 1010M microdensitometer
and a Philips CM300-FEG electron microscope. Salary is commensurate
with relevant experience. Successful candidates will join a strong
Program in Structural Biology with 4 groups using primarily X-ray
diffraction, 3 using NMR, 2 using EPR and one using EM. The Program
enjoys close ties with the National High Field Magnetic Laboratory and
the Supercomputer Computation Research Institute on campus. Additional
information about the Structural Biology Program can be found at
http://www.sb.fsu.edu/. Interested applicants should send their CV and
names, addresses and phone numbers of 3 references to Dr. Kenneth A.
Taylor, Institute of Molecular Biophysics, Florida State University,
Tallahassee, FL 32306, USA. E-mail address is taylor-at-bio.fsu.edu.
Phone number 1-850-644-3357, FAX 1-850-561-1406.


The Institute of Molecular Biophysics and Florida State University are
located in Tallahassee, the capitol of Florida. The city has a
population of ~200,000. The city is surrounded by rolling hills and
pine forests and is 35 miles from pristine beaches on the coast of Gulf
of Mexico. Tallahassee residents enjoy many cultural and sporting
events.



{/smaller} {/fontfamily}
{ { { {} } { { { {} } { { { {} } { { { {} } { { { {} } { { { {} } { { { {} } { { { {} } { { { {} } { { { {} } { { { {} } { { { {} } { { { {} } { { { {} } { { { {} } { { { {} } { { { {}


Kenneth A. Taylor, Ph.D. Office phone: 850-644-3357

Institute of Molecular Biophysics Lab phone: 850-644-4104

Florida State University EM room phone: 850-644-8769

Tallahassee, FL 32306-4380 Fax: 850-561-1406

E-mail: taylor-at-bio.fsu.edu

Home pages: http://www.sb.fsu.edu/~taylor/

http://www.fsu.edu/~biology/faculty/Taylor/kat.html


{ { { {} } { { { {} } { { { {} } { { { {} } { { { {} } { { { {} } { { { {} } { { { {} } { { { {} } { { { {} } { { { {} } { { { {} } { { { {} } { { { {} } { { { {} } { { { {} } { { { {}





{/x-rich}



From daemon Tue Jan 11 18:01:13 2000



From: wft03-at-health.state.ny.us
Date: Tue, 11 Jan 2000 16:25:35 -0500
Subject: Re: Osmium Vapor- A Safety Question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html





Posted at author's request to remain anonymous....

Dear Anon,

} Osmium Vapor- A Safety Question
}
} The last time I mentioned a safety concern about the rumor of an possible
} "explosive" warning about some Ruthenium compound , I got "Battered"...
}
The EM Safety Handbook also warns of the risk of explosion from RuO4.

} On approximately January 6, 2000 I noticed a request under the
} title:
}
} : Donna Wagahoff {DWAGAHOFF-at-siumed.edu}
}
} "....Does anyone have experience with osmium stored in
} plastic?..."
}
} and the only responses on the list server were:
}
} " How about putting the glass containers inside plastic containers?
} There are also padded and styrofoam containers which you could use for
} the transportation step."
}
} and
}
} ......" I store this glass bottle inside an aluminum-foiled plastic
} container in my fume hood at room
} temp. The clear plastic of this outer container gets translucent black
} within weeks no matter how carefully I seal the glass bottle".......
}
} ( I'm sure these were very fine suggestions..but..)
}
} This concerns me for several reasons:
}
} 1. I believe there is a considerably more dangerous situation here than
} many of us may realize, or..will discuss. We must check, and we may find
} that Osmium and Ruthenium compounds(and others?) may penetrate all
} plastics...
}
This is very likely.

} 2. There doesn't seem to be an acceptable safe level of these compounds
} or known affects, but probably fixation or contamination and loss of
} function at some level occurs(microscopic?).
}
The EM Safety Handbook gives a TLV--threshold limit value, defined as
a concentration
"to which *it is believed* (emphasis is mine) *nearly* all workers may be
repeatedly exposed day
after day without adverse effects."--of 2parts in 10^10. Note the very
small value and the cautionary
wording.

} 3.Also venting these vapors through hoods should be scrubbed or
} absorbed(?), reduced,etc.( "I'm no chemist") and the level of the output
} probably should be carefully and technically monitored.
}
Since OsO4 is a powerful oxidant, and since it reacts with unsaturated
lipids, I had heard
the recommendation that corn oil be used to clean up spills; however, the
EM Safety Handbook
recommends ascorbate powder "as it reacts quickly", and, come to think of
it, is more suited for
treating an aqueous solution than is an immiscible oil.

} 4. The safety level must be obvious to those who receive bottles of
} solutions in sealed glass(or Pirex?) and the factories that manufactor
} them.(What glass type materials are safe?)
}
I doubt that any particular glass is less safe (but would not be
surprised to be corrected).
Any glass which doesn't oxidize shouldn't react with OsO4.

} Does anyone know of the level of danger(or safety) or know of some good
} research sites or periodicals that have dealt with these issues? Any
} personal experience, or are we all afraid of retribution and retaliation?

The extensively-quoted EM Safety Handbook is a good source.

} The MSDS's seem a little less than thorough to me.

Although, the existance of an MSDS for di-hydrogen oxide (flamed here
some time ago)
would seem to argue otherwise. There is further info available from
company web sites and phone
lines (listed in some instances on the MSDS), and your safety office should
have other relevant info.

} I'm sure I'm again not
} alone here in this concern and only wish to continue this difficult and
} frequently dangerous craft(EM) with more care for myself , my associates
} and my community.
}
} Anonymous (chicken....)
} (...and Vendors and manufacturers are our backbone and our support!
} Thank you all!)
}
I'd say the first order of safety is to be concerned, next is to get
all the info available.
Yours in chickenhood,
Bill Tivol





From daemon Tue Jan 11 20:28:02 2000



From: c j day :      wa5ekh-at-juno.com
Date: Tue, 11 Jan 2000 19:55:07 -0600
Subject: Vapor Stains-Safety Issue Discussion Please

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


This technology needs more thorough discussion, I believe. There are
several rather serious safety issues that, I believe, we might find some
better solutions to here, if we open this area for discussion . For
example vapor level and detection, penetration, permeability, scrubbing,
reactive absorption, isolation, etc.. Some plastics stain easily and some
do not, but all are apparently permeable.
On the productive side the commercially astute might find several
new product ideas valuable to this community.
'JD'

previously:

Plastic is oxidized and so part of the osmium is exhausted in the vials.
Even
when frozen osmium diffuses through plastic containers. So you may save a
rare
breakage, but you are certain to have osmium in your refrigerator. It's
just a
bad idea to pack osmium in plastic. You could use a secondary container,
be it
plastic or glass, to increase overall safety.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Thursday, January 06, 2000 3:08 AM, Donna Wagahoff
[SMTP:DWAGAHOFF-at-siumed.edu] wrote:
}
} We have always kept our osmium solutions in glass containers. However,
we
} are in the process of evaluating our safety procedures and discussed
the
} idea of increasing the safety of the transport of osmium from the
} refrigerator to the fume hood by putting the osmium solution in plastic
} containers. (If they are dropped, they would not break and cause the
danger
} of a spill outside the hood.)
} Does anyone have experience with osmium stored in plastic? Any
comments
} about this particular subject or any of your safety with osmium
procedures
} are welcomed.
} Thanks.
}
} Donna Wagahoff
} SIU School of Medicine
} PO Box 19627
} Springfield, IL 62794-9627
} 217-782-0898
} fax217-524-3227





From daemon Tue Jan 11 20:41:33 2000



From: c j day :      wa5ekh-at-juno.com
Date: Tue, 11 Jan 2000 20:24:00 -0600
Subject: ESEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Recently an E3 Electroscan has become available and I'm becoming aware
of a much different SEM technology than I'm used to seeing over the last
30 years. It obviously operates only at vacuum levels in the torr range,
right? I am still a little confused how resolution can be maintained in
these vacuum levels. And dispersive X-ray spectroscopy, does this
broaden the peaks and what happens to spectral resolution? It also
appears that this particular design cannot pump down below 10-4(?).
There are some complex gas background issues that are different. Are
there ways of using these design parameters to the benefit of the
materials imaging analysis in samples that are not hydrated or partially
volatile?
'JD'/Texas





From daemon Wed Jan 12 07:38:15 2000



From: Krzysztof Jan Huebner :      hubner-at-IOd.krakow.pl
Date: Wed, 12 Jan 2000 11:50:25 +0100 (MET)
Subject: ICVGIP2000 (fwd)

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Our apologies if you receive multiple copies of this announcement.
Please circulate this announcement to your friends and other
researchers.

--------------------------------------------------------------------
Indian Conference on Computer Vision, Graphics and
Image Processing

Dec 20-22, 2000
Bangalore, INDIA

Email: icvgip-at-cair.res.in
URL: http://www.cair.res.in/~icvgip
Phone: +91 80 226 5609 Fax: +91 80 225 5615
--------------------------------------------------------------------

Call for Participation
----------------------

Continuing in the line of ICPIC '95 held at IIT, Kharagpur and
ICVGIP '98 held at IIT, Delhi, ICVGIP 2000 will be organized by the
Centre for Artificial Intelligence & Robotics at Bangalore during
December 20-22, 2000. The conference is intended to bring together
the Vision, Graphics, and Image Processing communities together with
a special emphasis on India. A high quality technical track will be
augmented by presentations from various R&D institutions in the
country and the industry.

Important Dates:
----------------

Submissions due: May 15, 2000
Notifiation of acceptance: Sep 01, 2000
Final papers due: Oct 15, 2000
Conference dates: Dec 20-22, 2000

Topics:
-------

We strive to host a high quality conference in India. An additional
goal his to bring the community of Indian practitioners of these
areas together at a single forum. We encourage papers related to
system development, innovative applications etc., in addition to
research papers. We especially encourage papers by student. The
topics of interest include, but are not limited to, the following:

Computer Vision Image Processing
Computer Graphics Signal Processing
Virtual Reality Multimedia
Document Analysis Pattern Recognition & Matching
Applications Image Processing Architectures
ASIC Design Software & Hardware Tools


Submissions:
------------

Electronic submissions are highly encouraged. Acceptable formats
are: Acrobat PDF, standard PostScript, self-contained LaTeX with
psfig, and Word 7.0. Check the official web page for details on
electronic submission. Manuscripts should not exceed 20
double-spaced pages including figures and tables. The submission
should include a cover page with the title, the authors' names,
abstract and keywords. Those submitting hard-copy manuscripts
should send four copies to the following address:

ICVGIP 2000 Secretariat
Centre for Artificial Intelligence & Robotics (CAIR)
Raj Bhavan Circle, High Grounds
Bangalore, 560 001. INDIA

Further Information:
--------------------

Email address: icvgip-at-cair.res.in
URL: http://www.cair.res.in/~icvgip
Fax: +91 80 225 5615 (Attn: ICVGIP 2000)

--------------------------------------------------------------------
Patrons:
--------
Prof. M. Vidyasagar, CAIR
Prof. R. Narasimha, NIAS

General Chair:
--------------
Dr. P. J. Narayanan, CAIR pjn-at-cair.res.in

Program Co-Chairs:
------------------
Prof. Ramakant Nevatia, USC nevatia-at-usc.edu
Prof. Jayanta Mukherjee, IIT, KGP jay-at-cse.iitkgp.ernet.in

Organizing Chair:
-----------------
Prof. Swamy Manohar, IISc manohar-at-csa.iisc.ernet.in

Plenary Chair:
--------------
Dr. P. Anandan, Microsoft anandan-at-microsoft.com

Publications Chair:
-------------------
Dr. C. V. Jawahar, CAIR jawahar-at-cair.res.in

Treasurer:
----------
Dr. Subrata Rakshit, CAIR subrata-at-cair.res.in

--------------------------------------------------------------------
Organized by Centre for Artificial Intelligence and Robotics (CAIR)
--------------------------------------------------------------------




From daemon Wed Jan 12 07:38:15 2000



From: Greg Ketley :      greg.ketley-at-snet.net
Date: Wed, 12 Jan 2000 06:18:28 -0500
Subject: Denton coater service

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Hi all-
I am looking for someone to provide in house service for a Denton
Sputter Coater in the Rhode Island area. Please feel free to contact me
offline.

Thanks
Greg Ketley
greg.ketley-at-snet.net



From daemon Wed Jan 12 07:38:16 2000



From: Frank Thomas :      thomasf-at-AGC.BIO.NS.CA
Date: Wed, 12 Jan 2000 08:41:30 -0400
Subject: Re: ESEM (longish)

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----------
} From: c j day {wa5ekh-at-juno.com}
} To: Microscopy-at-sparc5.microscopy.com
} Subject: ESEM
} Date: January 11, 2000 10:24 PM
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Recently an E3 Electroscan has become available and I'm becoming aware
} of a much different SEM technology than I'm used to seeing over the last
} 30 years. It obviously operates only at vacuum levels in the torr range,
} right? I am still a little confused how resolution can be maintained in
} these vacuum levels. And dispersive X-ray spectroscopy, does this
} broaden the peaks and what happens to spectral resolution? It also
} appears that this particular design cannot pump down below 10-4(?).
} There are some complex gas background issues that are different. Are
} there ways of using these design parameters to the benefit of the
} materials imaging analysis in samples that are not hydrated or partially
} volatile?
} 'JD'/Texas
}
} This is exactly the model we've been using here for the past 7 or so
years. In fact, the instrument can be operated in "normal" high vacuum
mode, too (if your samples don't mind it). The resolution is not bad, even
in "wet" mode - say, 2 - 10 torr. There is a certain amount of beam
diffusion, of course, in a wet atmosphere, but we've been doing EDS for the
past 5 or 6 years with pretty good, reproducible, results. (We have a NORAN
ultra-thin window detector and Voyager 3 software.)
Since we have a LaB6 gun in ours, there is also an ion pump, and the gun
vacuum is maintained at a little better than 10 -6 torr.
As you know, the instrument can be used with, instead of water vapour,
inert gases as an atmosphere in the chamber. As it happens, we don't do
that with our instrument, so I couldn't really comment. I suspect that
there probably aren't any major advantages in using an instrument like
this for materials studies, except that it has a very large specimen
chamber that can accomodate several types of stage. And, of course, the
fact that samples generally require no coating before examination. Much of
our usage is earth sciences, and it's nice not to have to coat type fossils
with carbon or metals.
There are two major disadvantages with the E3's. One is that the field of
view is very small - you can't really image anything larger than about 1 mm
in length or diameter, because of the configuration of the ESD
detector/final aperture assembly. This can be a major pain. Another is
that, with the standard stage, samples can not be thicker than about 25 mm
or so, especially if you want to do EDS.
FWIW, our instrument has been dead reliable since it's installation. Other
than biannual column cleanings, the odd hose leak, and an occasional glitch
with some miscellaneous part, the machine is hardly ever down. (Kind of
like my Harley - there may even be a few shared parts :-).
If you do wind up acquiring the E3, the Philips service rep for the
American southeast is (or at least was a couple years ago) Steve Booth.
(You probably know that Philips bought out ElectroScan a few years ago, so
they handle the service contracts now). Steve was here twice to do service
calls on ours, because both times, the regular US Northeast guy was
unavailable. Steve runs a horse ranch somewhere in Texas, I believe, but
knows his E3's pretty well, too.
This might be a whole lot more than you wanted to know, but I'll admit to
being a bit of a fan of our instrument - like my bike, it's ruggedly built,
but is no more complex than it has to be. Just last week, myself and a
local SEM service tech who'd never seen an ESEM before completed a biannual
column cleaning, and it all went very well - takes a day or two to get the
gun vacuum down to operating levels again, though.


No connection with Philips Electron Optics, etc......

F.C. Thomas
MicroAnalysis Facility
Geological Survey of Canada
Bedford Institute of Oceanography
Dartmouth, Nova Scotia
Canada
B3L 4C8



From daemon Wed Jan 12 08:20:05 2000



From: Giles Sanders :      g.sanders-at-ic.ac.uk
Date: Wed, 12 Jan 2000 07:44:17 -0600
Subject: Fw: CCD Video camera

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Dear List, can you help me once again. Can anyone recommend a sub $1500
video camera for video microscopy.  It will be generally used for
relatively high intensity fluorescence microscopy (imaging GFP bacteria)
and basic microscopy. At present we have a Sony XC-999P (752x582 pixels)
and are looking to upgrade - preferably in resolution and sensitivity -
but resolution is the most important factor. If people wish they can
respond off=list and I will produce a prŽcis of the information I receive.
Thanks.
--------------------------------------------------------------------------------
-----------------------Dr. Giles SandersZeneca / SmithKline Beecham Centre
for Analytical SciencesChemistry DepartmentImperial College of Science,
Technology and MedicineLondonSW7 2AY (44) - 0171-594-5749 Never
express yourself more clearly than you think. --  Niels Bohr
(1885-1962) Danish physicist
--------------------------------------------------------------------------------
--------------------





From daemon Wed Jan 12 17:31:53 2000



From: Tracey M. Pepper :      tpepper-at-iastate.edu
Date: Wed, 12 Jan 2000 09:24:09 -0600
Subject: N'tl Geographic/Scharf

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Hello All!
I guess National Geographic Explorer Magazine will be showing some of
David Scharf's work in the next "Explorer" show on TBS (Turner Broadcasting
System-yes it's not the Braves or Clint Eastwood!) on January 16th. They
will show some of his work imaging parasites with the modified
SEM. Should be cool! I love it when they show electron microscopy on TV.
Just thought you might want to know!

Tracey Pepper
Supervisor
Bessey Microscopy Facility
Iowa State University
ph: 515-294-3872
fax: 515.294.1337




From daemon Wed Jan 12 17:31:54 2000



From: Heeschen, Bill (WA) :      WAHEESCHEN-at-dow.com
Date: Wed, 12 Jan 2000 10:38:50 -0500
Subject: ESEM

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A few of the simpler advantages of an ESEM (we have a model E3):
No need to coat a sample
saves a few minutes (at least)
allows for back-and-forth work with a light microscope
No need to pre-pump to remove volatiles - the differential pumping system
handles this (although your rough pump oil becomes your trap)
Gas evolution (degradation on heating, for example)is not a problem (see
above)
Aging/dynamic studies don't get compromised due to sample coating.
near-atmospheric pressure minimizes sublimation without cryogenics
You can study influence of water (swelling, for example)or other sample/gas
interactions
ESD detector is light-insensitive, so you can watch the sample as you
position it. Also as you poke/prod it with the micromanipulator option.


As for EDX, yes, there is a significant beam spread from the imaging gas. I
typically line up the region-of-interest, then dial the chamber pressure to
zero before collecting spectra. The spread is significantly reduced.

Without trying to touch off arguments, my practical experience is that above
about 20,000X I don't collect images worth writing home about. They are
useful, but not beautiful.

It's an instrument that fills an interesting niche, even for a materials
scientist. (The real forte' is biological/wet stuff. That's where the fun
really begins!)

Bill Heeschen
The Dow Chemical Company

-----Original Message-----
} From: c j day [mailto:wa5ekh-at-juno.com]
Sent: Tuesday, January 11, 2000 9:24 PM
To: Microscopy-at-Sparc5.Microscopy.Com


Recently an E3 Electroscan has become available and I'm becoming aware
of a much different SEM technology than I'm used to seeing over the last
30 years. It obviously operates only at vacuum levels in the torr range,
right? I am still a little confused how resolution can be maintained in
these vacuum levels. And dispersive X-ray spectroscopy, does this
broaden the peaks and what happens to spectral resolution? It also
appears that this particular design cannot pump down below 10-4(?).
There are some complex gas background issues that are different. Are
there ways of using these design parameters to the benefit of the
materials imaging analysis in samples that are not hydrated or partially
volatile?
'JD'/Texas





From daemon Wed Jan 12 17:32:03 2000



From: Bob Wise :      wise-at-vaxa.cis.uwosh.edu
Date: Wed, 12 Jan 2000 11:07:13 -0500
Subject: flat sections

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Does anyone have a method for flattening 0.5 um thick epoxy (Spurr)
sections for LM? We are using water pickup and mild heat drying onto glass
slides and that doesn't seem to be doing the trick.

TIA

Bob
Dr. Robert R. Wise
Department of Biology
University of Wisconsin Oshkosh
Oshkosh, WI 54901
(920) 424-3404
wise-at-uwosh.edu
http://www.uwosh.edu/departments/biology/wise/wise.html



From daemon Wed Jan 12 17:31:54 2000



From: Tom Phillips :      PhillipsT-at-missouri.edu
Date: Wed, 12 Jan 2000 10:09:29 -0600
Subject: TEM membrane lipid visualization

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I want to look at what I hypothesize is a densely packed array of
membranes in TEM. In routine osmium fixed, LR White and Epon
embedded specimens, the image is not overwhelming. I plan to try
ruthenium tetroxide ala the skin people looking at lamellar bodies.
I am wondering whether I am missing another obvious approach. Any
ideas gratefully accepted. Tom

Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)



From daemon Wed Jan 12 17:32:00 2000



From: John Balk :      balk-at-kjhsgi.me.jhu.edu
Date: Wed, 12 Jan 2000 13:18:10 -0400
Subject: TEM: preparation of gold foils

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Dear Listers,

I am currently working on preparing TEM foils of single crystalline gold.
The electropolishing was completely fruitless, until I tried Bernie Kestel's
solution "BK-2". This has worked wonders, giving a very smooth and shiny
surface. FYI, I use a Struers Tenupol twin-jet electropolisher, so my
conditions are slightly different than with the South Bay polisher. One
problem remains: the electron-transparent edges of the foil are very prone
to bending, and thus I have regions that are full of bend contours. This
isn't terribly surprising, since the gold is from a grown single crystal and
has been subjected only to about 1% plastic strain. Any ideas on reducing
the amount of bending, so that I can see the dislocations more easily? Are
there any specific profiles for foil perforations that will help keep the
edges rigid (other than a smooth, small hole)? Any ideas, either for
improving specimen prep, or for "fixing" foils I already have, would be
greatly appreciated.

Regards,

John
--
____________________________________
John Balk
200 Latrobe Hall
Johns Hopkins University
3400 N. Charles St.
Baltimore, MD 21218
ph: (410) 516-8284
fax: (410) 516-4316
e-mail: balk-at-kjhsgi.me.jhu.edu




From daemon Wed Jan 12 17:31:57 2000



From: Yuhui_Xu :      Yuhui_Xu-at-hms.harvard.edu
Date: Wed, 12 Jan 2000 12:18:24 -0500
Subject: CCD Cameras for TEM

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Dear Colleagues:

We are in the market for a CCD camera for a JEOL 1200CX TEM. I would
appreciate any information regarding to this type of products available on the
market.
Thank you.

Yuhui




From daemon Wed Jan 12 17:32:01 2000



From: Damian :      dneuberger-at-mindspring.com
Date: Wed, 12 Jan 2000 12:45:29 -0600
Subject: Re: Vapor Stains-Safety Issue Discussion Please

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Those of you who know me can understand that I agree completely with JDs
comment below. The whole issue of safety in handling laboratory chemicals
is one that, I believe, has still not been completely addressed and
accepted by everyone. We all know the short term effect that exposure to
toxic levels of OsO4 can have on a person, eyes is one that comes to
mind. Formaldehyde and glutaraldehyde are others that I and a pathologist,
who I just met yesterday, are now well aware of its possible long term
effects. But what about long term, synergistic effects of some chemicals
that are by themselves relatively innocuous? Combinations of two, three,
four? The only answer is to develop and follow safely procedures for
handling each chemical as if it were very toxic. There is no reason not
to, you already do it for some chemical, and there are many reasons to do so.

For those who don't know me and need a little motivation, think "bilateral,
single sequential lung transplant" .

Damian


At 07:55 PM 1/11/00 -0600, c j day wrote:

} This technology needs more thorough discussion, I believe. There are
} several rather serious safety issues that, I believe, we might find some
} better solutions to here, if we open this area for discussion . For
} example vapor level and detection, penetration, permeability, scrubbing,
} reactive absorption, isolation, etc.. Some plastics stain easily and some
} do not, but all are apparently permeable.
} On the productive side the commercially astute might find several
} new product ideas valuable to this community.
} 'JD'




From daemon Wed Jan 12 17:32:02 2000



From: Sally Stowe :      stowe-at-rsbs.anu.edu.au
Date: Thu, 13 Jan 2000 07:17:01 +1100
Subject: Re: Vapor Stains-Safety Issue

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What is the longterm effect of exposure to osmium on the brittleness and
permeability of common plastics? We leave exposed polymer block faces in
osmium vapour overnight before sectioning.


Sally



From daemon Wed Jan 12 17:32:02 2000



From: Robert Palmer :      rjpalmer-at-dir.nidcr.nih.gov
Date: Wed, 12 Jan 2000 15:27:11 -0500
Subject: ESEM in DC

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Hello all! We are looking for an ESEM in the greater Washington DC area
for a limited amount of work (maybe a dozen samples over a two-month
period). This could be a contractual situation, although we are rather
hoping for the owner's generosity....
Please reply off-list to: rjpalmer-at-dir.nidcr.nih.gov
Robert J. Palmer Jr., Ph.D.
Natl Inst Dental Craniofacial Res - Natl Insts Health
Oral Infection and Immunity Branch
Bldg 30, Room 310
30 Convent Drive
Bethesda MD 20892
ph 301-496-2088
fax 301-402-0396



From daemon Wed Jan 12 17:32:07 2000



From: Harrison, Gail :      Gail.Harrison-at-reichhold.com
Date: Wed, 12 Jan 2000 16:44:43 -0500
Subject: Optical Scope Needs Servicing

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I have a Reichert compound microscope that needs servicing. The number I
have for the company who used to service my microscope is no longer valid.
Is there anyone in the RTP, NC area who could recommend a service vendor?
Please respond to gail.harrison-at-reichhold.com

Many thanks in advance

Gail Harrison
Reichhold
RTP, NC



From daemon Thu Jan 13 07:47:04 2000



From: simon baconnier :      simonb-at-bgumail.bgu.ac.il
Date: Thu, 13 Jan 2000 15:32:39 +0200
Subject: Pineal gland

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Hi,

I'm a post graduate student, and i'm working on the pineal gland, a gland
located in the middle of the brain.

I'd like to know what would be the better way to prpare it for a thin cut
and a hard fixation??

Thank you for answering!!

Simon




From daemon Thu Jan 13 17:49:58 2000



From: Jaci Lett :      jmlett-at-cid.wustl.edu
Date: Thu, 13 Jan 2000 09:20:44 -0600
Subject: STAIN: Toluidine Blue removal

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Does anyone have a solution for this problem? I managed to get a spot ( {3mm
around) of Toluidine Blue on the cuff of my new shirt (the only part
extending beyond my lab coat). Is there any way to get rid of all (or most)
of the stain without it spreading? The shirt is 100% cotton. (I don't care
if it gets on my lab coat, but this is the first time in over fourteen years
that I've gotten it on my clothes.)

If I don't get any response, my inclination is to try a sodium tetraborate
paste applied with a cotton swab.

Jaclynn Lett, Research Assistant jmlett-at-cid.wustl.edu

Fay and Carl Simons Center for the Biology of Hearing and Deafness
Central Institute for the Deaf
818 S. Euclid Ave.
St. Louis, MO 63110

voice: 314-977-0257 fax: 314-977-0030




From daemon Thu Jan 13 17:50:02 2000



From: Downey, Kevin E :      Kevin.Downey-at-hrl.bsco.com
Date: Thu, 13 Jan 2000 13:42:47 -0500
Subject: TEM Extraction Replicas

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I am having great difficulty extracting large carbides from a steel
specimen using the standard single stage carbon extraction technique.
The film is either not releasing or it is breaking up into unusable
pieces. My standard practice is as follows:

1) Etch polished surface in 2% Nital for 15 to 45 seconds
2) Release sections in 10% Nital
3) Float sections in Dist. water and retrieve

I have used an electrolytic process to aid in releasing the sections
but often this process tends to create somewhat dirty replicas.

I also am going to try a two-stage replication technique, but would prefer
to have sucessful single stage replicas.

Does anyone have any suggestions that would be of assistance in my
single stage replication technique? Thank you for your consideration.

Kevin Downey
Research Analyst
Bethlehem Steel Corp.
e-mail: rkedo-at-bsco.com



From daemon Thu Jan 13 17:50:02 2000



From: pmoore-at-wfubmc.edu (Paula Moore)
Date: Thu, 13 Jan 2000 13:43:54 -0500
Subject: Re: STAIN: Toluidine Blue removal

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} Does anyone have a solution for this problem? I managed to get a spot ( {3mm
} around) of Toluidine Blue on the cuff of my new shirt (the only part
} extending beyond my lab coat). Is there any way to get rid of all (or most)
} of the stain without it spreading? The shirt is 100% cotton. (I don't care
} if it gets on my lab coat, but this is the first time in over fourteen years
} that I've gotten it on my clothes.)

We have some stuff called Erada-Stain. Its made for histological stain removal
from hands, glassware, etc. We've had our tube of it for forever(15 years +)
but it seems like it would be one of those things you should still be able to
find.
Its always worked for me when I had a clothes splash.
Good Luck

Paula Moore
Wake Forest Univ. Baptist Medical Center
EM Lab
pmoore-at-wfubmc.edu




From daemon Thu Jan 13 17:50:02 2000



From: White, Woody N :      Woody.N.White-at-mcdermott.com
Date: Thu, 13 Jan 2000 12:56:00 -0600
Subject: TIFF image dpi format ?

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Hello All,

Embeded in TIFF images is data describing the "print" size, X inches by Y
inches at N dpi. Of course, this is directly related to the pixel matrix
size.

My problem is that my digital imaging equipment (Hitachi S-3500 and IXRF
EDS)
saves the image data at 72 (or less) dpi. The pixel array size is correct,
but
at {= 72 dpi, many programs (like PhotoShop) want to print a huge image.

I can overcome this by (in P.S. for example) resetting the image to 300 dpi
and
adjusting the print size so that the pixel array is not altered. At the
very
least, this is cumbersome and time consuming when a large number of images
must
be printed.

I would like to find a way to change the file saving default value for the
dpi
to avoid image resizing for most print applications.

Any suggestions???

Thanks,
Woody White
McDermott Technology



From daemon Thu Jan 13 17:50:03 2000



From: Caroline Schooley :      schooley-at-mcn.org
Date: Thu, 13 Jan 2000 11:27:36 -0800 (PST)
Subject: Mattel QX3 toy microscope

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I've received a lot of inquiries about the education potential of the $100
(or less, in some stores) toy digital microscope introduced by Mattel just
before Christmas; it's a plastic-bodied scope with simple image processing
software which requires a wired connection to a Windows 98 computer (I'm a
Mac user and I don't often feel envious, but...). It's been hard to get
good information, but Jim Harper has just posted an excellent article on
the web. He describes its capabilities well - far better than any of the
other reviews that I've read. And he gives detailed instructions on how to
mount it on ANY light microscope! Don't miss the hotlinks at the end of
his piece.

Educationally, it's no substitute for the one student - one microscope
approach of Project MICRO and "Microscopic Explorations", its manual. But
it has exciting potential for classroom demonstrations and science fair
projects. And listserver readers who are looking for low cost digital
recording of LM may find that it's adequate for a lot of applications.
Please let us know if it works for you.

The site is http://www.microscopy-uk.org.uk/mag/artjan00/jhqx3.html

Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/PCI/pci.html





From daemon Thu Jan 13 17:50:06 2000



From: bozzola-at-siu.edu (John J. Bozzola)
Date: Thu, 13 Jan 2000 14:36:41 -0600
Subject: SYMPOSIUM CALL FOR PAPERS

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==============================================

ANNOUNCEMENT, AN INVITATION TO OUR SYMPOSIUM:

I am soliciting contributors (or names of potential contributors) for a
symposium for the natiional Microscopy & Microanalysis Annual Meeting to be
held on August 13-17, 2000 in Philadelphia, Pa.

Talks may range in length from 25 to 45 minutes. Deadline for receipt of 2
page absracts is Feb 15, 2000.

A description of the symposium follows.

SYMPOSIUM: MICROORGANISMS: THE GOOD, THE BAD, THE UNUSUAL

This symposium will deal with microorganisms (viruses, bacteria, parasites,
prions) found in the environment as well as in higher life forms (animals
and plants). Newly discovered pathogens or organisms with unique
capabilities (detoxification, invasiveness, resistance to antibiotics) are
of interest in this symposium. Of particular interest are those orgamisms
that represent extremes, as for example: the ability to grow in extreme
environments, having extreme virulence or invasiveness, or being difficult
to visualize using conventional prepartory procedures. Hopefully, the
participants shall describe some of the features of extreme organisms that
give rise to these capabilities. Finally, many of these organisms are often
difficult to visualize using standard preparatory procedures. Papers
describing procedures to prepare the specimens for visualization would be
germane to this symposium.


==============================================







####################################################################
John J. Bozzola, Ph.D., Director
Micro-Imaging and Analysis Center
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
####################################################################





From daemon Thu Jan 13 17:50:07 2000



From: Rick Harris :      raharris-at-ucdavis.edu
Date: Thu, 13 Jan 2000 13:31:24 -0800
Subject: Re: TIFF image dpi format ?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} Hello All,
}
} Embeded in TIFF images is data describing the "print" size, X inches by Y
} inches at N dpi. Of course, this is directly related to the pixel matrix
} size.
}
} My problem is that my digital imaging equipment (Hitachi S-3500 and IXRF
} EDS)
} saves the image data at 72 (or less) dpi. The pixel array size is correct,
} but
} at {= 72 dpi, many programs (like PhotoShop) want to print a huge image.
}
} I can overcome this by (in P.S. for example) resetting the image to 300 dpi
} and
} adjusting the print size so that the pixel array is not altered. At the
} very
} least, this is cumbersome and time consuming when a large number of images
} must
} be printed.
}
} I would like to find a way to change the file saving default value for the
} dpi
} to avoid image resizing for most print applications.
}

I also have a Hitachi S-3500N. Use the Action Palette in PhotoShop to
change the print size and adjust the pixel array with a single key stroke.



Rick A. Harris, Director
Microscopy and Imaging Facility
Section of Molecular and Cellular Biology
1241 Life Sciences Addition
University of California
Davis, CA
530 752 2914
530 754 7536 fax
http://katie.ucdavis.edu
raharris-at-ucdavis.edu



From daemon Thu Jan 13 17:50:09 2000



From: Walck. Scott D. :      walck-at-ppg.com
Date: Thu, 13 Jan 2000 18:12:08 -0500
Subject: RE: TIFF image dpi format ?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I suggest getting a hold of ThumbsPlus. I print everything from it. It can
stretch an image to full scale and can print annotation text with the image.
It is a graphics database program that works very well. In addition, it can
convert from almost any format to any other format.

What it can do for you is to convert your image from 72 dpi -big to 300
-small and keep it in the same format, e.g. Tiff. You can do a batch
convert easily.

Find out more at www.cerious.com

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)



} -----Original Message-----
} From: White, Woody N [mailto:Woody.N.White-at-mcdermott.com]
} Sent: Thursday, January 13, 2000 1:56 PM
} To: Microscopy-at-Sparc5.Microscopy.Com
} Subject: TIFF image dpi format ?
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.} html
}
}
} --------------------------------------------------------------
} ---------.
}
}
} Hello All,
}
} Embeded in TIFF images is data describing the "print" size,
} X inches by Y
} inches at N dpi. Of course, this is directly related to the
} pixel matrix
} size.
}
} My problem is that my digital imaging equipment (Hitachi
} S-3500 and IXRF
} EDS)
} saves the image data at 72 (or less) dpi. The pixel array
} size is correct,
} but
} at {= 72 dpi, many programs (like PhotoShop) want to print a
} huge image.
}
} I can overcome this by (in P.S. for example) resetting the
} image to 300 dpi
} and
} adjusting the print size so that the pixel array is not
} altered. At the
} very
} least, this is cumbersome and time consuming when a large
} number of images
} must
} be printed.
}
} I would like to find a way to change the file saving default
} value for the
} dpi
} to avoid image resizing for most print applications.
}
} Any suggestions???
}
} Thanks,
} Woody White
} McDermott Technology
}



From daemon Thu Jan 13 20:01:28 2000



From: Dave Phelan :      emudp-at-mail.newcastle.edu.au
Date: Fri, 14 Jan 2000 11:56:52 +1100
Subject: X-Ray mapping problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


G'day

I have a problem doing x-ray mapping on my Philips XL30 (W) with
an attached Oxford ISIS 200 EDS system. I'm running the ISIS
software on the same pc that controls the XL30, a 133 Pentium
with 32Mb memory, running NT4 with service pack 3. I have ISIS
v3.32 and XL v5.5 software. When I try to collect say 6 elemental
maps using the SPEEDMAP software the system locks after 2 or
3 scans and the computer has to be re-booted. I have tried
increasing the memory up to 80Mb but this had no effect. I did not
have this problem when running Windows 3.1, although the system
would give 'out of memory' errors which is why I upgraded to NT.
The problem also occurs when collecting an image using the ISIS
AUTOBEAM software and integrating several frames. Single frame
acquisitions are ok.
If you have a similar system configuration would you please let me
know it you experience this problem? I know of stand alone NT
systems that are ok, so can only assume it is some conflict with
my particular software/hardware combination.

Thanks

Dave







Dave Phelan
EM/X-Ray Unit
University of Newcastle
NSW 2308
AUSTRALIA
Ph 02 4921 5667
Fax 02 4921 7019
emudp-at-mail.newcastle.edu.au




From daemon Fri Jan 14 07:48:17 2000



From: Mel Dickson :      m.dickson-at-unsw.edu.au
Date: Fri, 14 Jan 2000 16:52:03 +1100
Subject: Hitachi FESEMs in NY

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello EM Listers,

We are having antifield systems from Lindgren RF Enclosures , fitted on our
two Hitachi FESEMs, an S-4500II and an S-900.

It would be very advisable for the installing engineer to inspect columns
of these models before they set out for Sydney.

Could any operator of these models around New York who would allow
inspection of their microscopes please mail me?

thanks,


Dr. Mel Dickson,
Deputy Director, The Electron Microscope Unit,
Adjunct Associate Professor, School of Microbiology & Immunology
The University of New South Wales Sydney, Australia.
Phone +612 9385 6383 Fax +612 9385 6400



From daemon Fri Jan 14 07:48:20 2000



From: Keith Ryan :      KPR-at-wpo.nerc.ac.uk
Date: Fri, 14 Jan 2000 09:24:55 +0000
Subject: gutaraldehyde safety

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Microscopists

Revisiting an old chestnut - safety and aldehyde fixatives.

In the UK, the Health & Safety Commission have just lowered exposure
limits for glutaraldehyde.

Formalin/formaldehyde has a MEL (maximum exposure limit) of 2 ppm or
2.5 mg/cu m - this is measurable and legally enforcible.
Glutaraldehyde used to have an OES (occupational exposure standard) of
0.2 ppm or 0.83 mg/cu m. over a 15 minute period. OES is a standard to
aim for, but not prosecutable if you weren't achieving it.

Now, glutaraldehyde suddenly has a MEL of 0.05 ppm, both for 15
minute short term reference limit AND THE 8 HOUR TIME WEIGHTED AVERAGE
EXPOSURE!! This is a quarter of what it previously was and 40 times
lower than that for formaldehyde! Does anyone know why or have
evidence or anecdotes of glutaradehyde-related health problems? Is it
so nasty??

I appreciate that it is used in bulk as a bacteriocide in hospitals
and possibly in horticulture/agriculture.

I am trying to contact HSC specialist committees for a response and
will post anything that I receive. I suspect that may be very
little.

Regards - Keith (reincarnated after "early retirement")

PS - Hello, Daniele! And those who know her!
_______________________________
Dr. Keith Ryan
Marine Biological Association of the UK
The Laboratory
Citadel Hill
Plymouth
Devon PL1 2PB
England

Tel. ++44 (0)1752 633249
Tel. ++44 (0)1752 633279
The 279 number has an answering machine

Fax ++44 (0)1752 633102

e-mail: kpr-at-wpo.nerc.ac.uk




From daemon Fri Jan 14 07:48:23 2000



From: White, Woody N :      Woody.N.White-at-mcdermott.com
Date: Fri, 14 Jan 2000 07:34:00 -0600
Subject: TIFF dpi follow-up...

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thanks for the replies, but...

There may be some confusion about my question. Perhaps I can clarify....

I can resize/fix the image size parameters ok. Either totally manually or
with
a macro from PhotoShop, etc.

My goal is to NOT have to do that. If the original images are SAVED at the
appropriate print dpi this would not be required.... That is my goal. That
is... Is there any way to modify the SAVING software (Hitachi PC-SEM/IXRF
Iridium) so that the images are, for example, 300 dpi rather than 72 or 26
which
is what the software(s) does now.

Woody



From daemon Fri Jan 14 18:31:54 2000



From: HILDEGARD CROWLEY :      hcrowley-at-du.edu
Date: Fri, 14 Jan 2000 09:57:38 -0700 (MST)
Subject: Re: STAIN: Toluidine Blue removal

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




On Thu, 13 Jan 2000, Paula Moore wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} } Does anyone have a solution for this problem? I managed to get a spot ( {3mm
} } around) of Toluidine Blue on the cuff of my new shirt (the only part
} } extending beyond my lab coat). Is there any way to get rid of all (or most)
} } of the stain without it spreading? The shirt is 100% cotton. (I don't care
} } if it gets on my lab coat, but this is the first time in over fourteen years
} } that I've gotten it on my clothes.)
}
} We have some stuff called Erada-Stain. Its made for histological stain removal
} from hands, glassware, etc. We've had our tube of it for forever(15 years +)
} but it seems like it would be one of those things you should still be able to
} find.
} Its always worked for me when I had a clothes splash.
} Good Luck
}
} Paula Moore
} Wake Forest Univ. Baptist Medical Center
} EM Lab
} pmoore-at-wfubmc.edu
}
}
}
Hi,

To destain a slide which has been contrasted with toluidine blue (or any
of the other blues), soak the slide in acid alcohol. To one liter of 70%
ethanol, add 10ml of conc hydrochloric acid. Soak the cuff in that. If
that does not do it, paint your cuff. I have done this frequently. Use
laundry marking stick or magic marker or whatever. If I have a lot of
staining to do, I
simply wear a multicolor blue blouse. No problem.

Hildy Crowley,




From daemon Sun Jan 16 07:07:06 2000



From: HILDEGARD CROWLEY :      hcrowley-at-du.edu
Date: Fri, 14 Jan 2000 09:57:38 -0700 (MST)
Subject: Re: STAIN: Toluidine Blue removal

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




On Thu, 13 Jan 2000, Paula Moore wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} } Does anyone have a solution for this problem? I managed to get a spot ( {3mm
} } around) of Toluidine Blue on the cuff of my new shirt (the only part
} } extending beyond my lab coat). Is there any way to get rid of all (or most)
} } of the stain without it spreading? The shirt is 100% cotton. (I don't care
} } if it gets on my lab coat, but this is the first time in over fourteen years
} } that I've gotten it on my clothes.)
}
} We have some stuff called Erada-Stain. Its made for histological stain removal
} from hands, glassware, etc. We've had our tube of it for forever(15 years +)
} but it seems like it would be one of those things you should still be able to
} find.
} Its always worked for me when I had a clothes splash.
} Good Luck
}
} Paula Moore
} Wake Forest Univ. Baptist Medical Center
} EM Lab
} pmoore-at-wfubmc.edu
}
}
}
Hi,

To destain a slide which has been contrasted with toluidine blue (or any
of the other blues), soak the slide in acid alcohol. To one liter of 70%
ethanol, add 10ml of conc hydrochloric acid. Soak the cuff in that. If
that does not do it, paint your cuff. I have done this frequently. Use
laundry marking stick or magic marker or whatever. If I have a lot of
staining to do, I
simply wear a multicolor blue blouse. No problem.

Hildy Crowley,




From daemon Fri Jan 14 18:31:58 2000



From: Linda Fox :      LFOX1-at-wpo.it.luc.edu
Date: Fri, 14 Jan 2000 13:43:26 -0600
Subject: Barr bodies in TEM prep

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greetings friends,
A researcher wants to know how to stain for, or find by TEM, Barr
bodies that are already prepped and embedded in resin for TEM. Any
advise that you can supply will be most appreciated.
Thanks,
Linda M. Fox
Loyola University
Stritch School of Medicine
Core Imaging Facility
2160 S. First Ave.
Maywood, Il 60153
Bld. 102 Room 0617
1-708-216-3395
lfox1-at-wpo.it.luc.edu



From daemon Fri Jan 14 18:31:59 2000



From: Larry Stoter :      LPS-at-teknesis.demon.co.uk
Date: Fri, 14 Jan 2000 22:01:18 +0000
Subject: Re: TEM Extraction Replicas

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

I spent a lot of time many years ago working on 316 stainless. Many
of the specimens I made were single stage carbon extraction replicas.
The technique I found most effective was to use dilute hydrochloric
acid and electrolytic activation. I used this both to etch the
surface prior to carbon coating and also to release the carbon
replica. I also used the same process on ferritic steels. It was very
effective it even released large sheets of M23C6 carbides from grain
boundaries. The only 'precipitate' it would not work on was ferrite
in austenite.

regards,
--
Larry Stoter
17, Rocks Park Road, Uckfield, E. Sussex, TN22 2AT, UK
email: LPS-at-teknesis.demon.co.uk, Home Phone/Fax: +44 (0)1825 767967



From daemon Sun Jan 16 07:06:55 2000



From: Sally Stowe :      stowe-at-rsbs.anu.edu.au
Date: Sat, 15 Jan 2000 11:45:00 +1100
Subject: EM Position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Position open at the Australian National University , Canberra
www.anu.edu.au/hr/jobs

RESEARCH SCHOOL OF BIOLOGICAL SCIENCES
ELECTRON MICROSCOPY UNIT
ELECTRON MICROSCOPIST
ANU OFFICER GRADE 7 (TECHNICAL)
$43,506 - $47,010 per annum (Plus generous superannuation
provisions)

Reference No: G000011. The ANU Electron Microscopy Unit, a
multidisciplinary research and teaching
support facility with four SEMs, three TEMS and ancillary equipment,
requires a skilled and experienced
person to join its team of 5-6 staff. The successful applicant will have a
history of work in electron
microscopy in a diversified research-oriented environment, preferably with
some administrative experience,
and areas of expertise that support and complement those of existing staff.
They will have up-to-date
expertise in a number of areas of electron microscopy and image analysis.
Among these, experience with
quantitative energy-dispersive X-ray analysis, research projects in plant
or animal cell biology, and
cryopreparation techniques is a necessity.

The ANU EMU website is http://online.anu.edu.au/EMU

Contact for selection documentation: Ms Susan Toscan , ph (02) 6249 4752,
email:
susan.toscan-at-rsbs.anu.edu.au
For further information contact: Dr Sally Stowe, email:
stowe-at-rsbs.anu.edu.au
Closing date: 31st January 2000.




From daemon Sat Jan 15 06:23:38 2000



From: Shaffer-at-physics.niu.edu
Date: Fri, 14 Jan 2000 20:30:14 -0600
Subject: Tenure-Track Faculty Position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



NORTHERN ILLINOIS UNIVERSITY

Tenure-Track Faculty Position in Condensed Matter: Electron
Microscopist. The candidate should have a strong background in
transmission electron microscopy and diffraction, and an interest in the
applications of advanced TEM techniques to materials physics. The
candidate would be expected to have a broad knowledge of electron
diffraction theory, of high resolution microscopy and electron
spectroscopy and of materials physics. Possible areas of interest
include (but are not limited to), microscopy of magnetic and/or
superconducting materials, including holography; defects and interfaces
in materials; ferroelectrics; diamond films and film growth; nanoscale
materials; amorphous materials; quantitative microscopy; and 3-D
tomography. Although not essential, an interest in electron optics
would be valuable. The candidate will have the opportunity to establish
a joint program with the Electron Microscopy Center in The Materials
Science Division at Argonne National Laboratory. Send curriculum vitae
and references by March 17, 2000 to: Physics Dept., NIU, DeKalb, IL
60115, Attn: J. C. Shaffer, Chairman. NIU is an AA/EEO Institution.






From daemon Sat Jan 15 06:23:40 2000



From: jean michel Wulveryck :      jm.wulveryck-at-univ-reims.fr
Date: Sat, 15 Jan 2000 15:36:39 +0100
Subject: Information about Beam blanking ....

Contents Retrieved from Microscopy Listserver Archives
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Try Scripps their metrology does contract AFM.

Scripps Institution of Oceanography Analytical Facility

http://sioaf.ucsd.edu/flyer/



----- Original Message -----
} From: {"rfelten-at-Macdermid.com"-at-sparc5.microscopy.com}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Wednesday, January 05, 2000 2:16 PM


Dear colleagues,

Would you please give us some information about a beam blanking device
and a cryostat which can be set inside the microscope chamber. In fact,
we would like to modify our old Phillips microscope (SEM 505) with these
two devices. In particularly, could you give us the quotation for these
two devices,

Thanking you in advance,
Cordially,

email adresse for the answer :
abdelillah.elhdiy-at-univ-reims.fr




From daemon Mon Jan 17 07:16:46 2000



From: Caroline Schooley :      schooley-at-mcn.org
Date: Sun, 16 Jan 2000 09:44:33 -0800 (PST)
Subject: Mattel QX3 toy microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I've received a lot of inquiries about the education potential of the $100
(or less, in some stores) toy digital microscope introduced by Mattel just
before Christmas; it's a plastic-bodied scope with simple image processing
software which requires a wired connection to a Windows 98 computer (I'm a
Mac user and I don't often feel envious, but...). It's been hard to get
good information, but Jim Harper has just posted an excellent article on
the web. He describes its capabilities well - far better than any of the
other reviews that I've read. And he gives detailed instructions on how to
mount it on ANY light microscope! Don't miss the hotlinks at the end of his
piece.

Educationally, it's no substitute for the one student - one microscope
approach of Project MICRO and "Microscopic Explorations", its manual. But
it has exciting potential for classroom demonstrations and science fair
projects. And listserver readers who are looking for low cost digital
recording of LM may find that it's adequate for a lot of applications.
Please let us know if it works for you.

The site is http://www.microscopy-uk.org.uk/mag/artjan00/jhqx3.html

Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/PCI/pci.html





From daemon Mon Jan 17 07:16:54 2000



From: Mel Dickson :      m.dickson-at-unsw.edu.au
Date: Mon, 17 Jan 2000 17:32:50 +1100
Subject: position open

Contents Retrieved from Microscopy Listserver Archives
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University of New South Wales,
Sydney, Australia

ELECTRON MICROSCOPE UNIT

Laboratory Assistant

REF. 063NET

FIXED TERM - Total Remuneration: Level 3 (38 hours): A$35,824 - A$41,251
per year. (Salary Level 3: A$30,272 - A$34,858 per year plus up to 17%
employer superannuation plus leave
loading.)

The Electron Microscope Unit is a central infrastructural research
facility, containing nine principal instruments, which support a wide range
of projects. The Unit seeks a self-motivated, enthusiastic person to offer
technical support to assist in the smooth running of the Unit. The
successful applicant will be required to perform a range of routine
laboratory tasks such as film processing, specimen preparation and assist
users of the Unit with operation of microscopes.

Essential criteria: familiarity with the operation of both scanning and
transmission
electron microscopes and with microscope specimen preparation techniques;
previous
experience in research laboratory environment; familiarity with common
windows-based
software packages; good interpersonal skills and a knowledge of EEO/AA
principles.

Desirable criteria: experience with microscopy of biomedical specimens,
experience with
cryomicroscopy techniques; ability to use image processing and analysis
software.
This is a fixed term position to 31 December 2000

Information about the Unit can be found on its website:
http://srv.emunit.unsw.edu.au

Enquiries may be directed to Associate Professor Paul Munroe on telephone
(02) 9385 4435, facsimile (02) 9385 6400 or email: p.munroe-at-unsw.edu.au.

Applications close 28 January 2000.

Dr. Mel Dickson,
Deputy Director, The Electron Microscope Unit,
Adjunct Associate Professor, School of Microbiology & Immunology
The University of New South Wales Sydney, Australia.
Phone +612 9385 6383 Fax +612 9385 6400



From daemon Mon Jan 17 07:29:13 2000



From: Alex_Liversage-at-bio-rad.com
Date: Mon, 17 Jan 2000 07:17:37 -0600
Subject: problematic immunogold labelling references

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear readers


I have been looking for some relevant references to put in my PhD
thesis which are applicable to the work undertaken.

I was trying to immunogold label (using 5nm gold conjugated to Fab) an
epitope on the giant muscle protein titin within muscle fibre bundles
(all Ab labelling was done prior to sample fixation). However, the
labelling seen was low and inconsistent.

Labelling with FITC conjugated Ab or unconjugated Ab labelled samples
which were then stained was fine though.

Does anyone one know of similar work where immunogold labelling has
failed and possible reasons for this has been explicitly mentioned
within the paper.

Many thanks


Alex Liversage





From daemon Mon Jan 17 19:12:52 2000



From: Roger Moretz :      rcmoretz-at-excite.com
Date: Mon, 17 Jan 2000 09:48:05 -0800 (PST)
Subject: Plan Apo Objectives for Leitz Aristoplan

Contents Retrieved from Microscopy Listserver Archives
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I have inherited a Leitz Aristoplan microscope for integration into a
digital imaging system for histopathology. Unfortunately, the mid-range
objectives (10, 25, 40x) are all fluors, although the scope is not equipped
for fluorescence. I would like to acquire planapochromats for each of these
magnifications. The Aristoplan is a fixed tube length (160mm) instrument
that is excellent optically, but the fluotars cause significant vignetting
at all magnifications, even with the correct C mount. If you can provide
any or all of these lenses, please contact me off-list with pricing
information and purchasing details.

Roger Moretz
Dept of Toxicology





_______________________________________________________
Get 100% FREE Internet Access powered by Excite
Visit http://freeworld.excite.com




From daemon Mon Jan 17 19:12:54 2000



From: ancq3f1nkrz5xza-at-lll.de
Date: Mon, 17 Jan 2000 12:34:37
Subject: Need To Reach Thousands Of Prospects Every Month?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


_/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/

S P E C I A L R E P O R T -

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_/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/

Our research has found that many people have tried one or more of
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Free Classifieds? (They just don't work anymore)
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S O W H A T D O E S W O R K ?

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For those not interested in this research, or, if you're
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5 1,050 4,200 50,400
10 2,100 8,400 100,000
15 3,150 12,600 151,200


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_/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/

F R E E B O N U S E S

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_/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/

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O R D E R N O W . . . SAME DAY SERVICE (M-F) if your order
is received before 2pm Pacific. 24hour fax service, just fax
to: 1-435-404-1340

To order, via credit card simply cut/paste and print out the
EZ ORDER FORM below and fax to our office today.

***** MILLIONS CD - Volume 7 *****

***** NOW ONLY $235! *****

This "Special Price" is in effect for the next seven days,
after that we go back to our regular price of $299.00 ...
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We accept Visa, Mastercard, Amex and Checks by Fax.
Fax your order to: 1-435-404-1340

----------------------Cut & Paste----------------------
---------------------EZ Order Form---------------------

_____Yes! I want everything! I am ordering within 72 hours.
Include my FREE "Business On A CD" bonus along with your 10
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addresses) for the special price of only $235.00 + s&h.

_____I missed the 72 hour special, but I am ordering Vol. 7,
10 Million, super clean e-mail addresses, within 7 days for the
"special" price of only $235.00 + s&h.

_____Oop's I missed the 72 hour and 7 day "specials". I am
ordering Vol. 7 at the regular price of $299.00 + s&h.

***PLEASE SELECT YOUR SHIPPING OPTION***

____I would like to receive my package FedEx OVERNIGHT. I am
including $15 for shipping. (Hawaii & Alaska $20 - Canada $25,
all other International add an *additional* $25 [$40 total] for
shipping)

____I would like to receive my package FedEx 2 DAY delivery.
I'm including $10 for shipping. (Sorry no Alaska, Hawaii, Canada
or International 2nd day delivery - Continental U.S. shipping
addresses only).

***Please Print Carefully***

NOTE: Orders cannot be shipped without complete information
including your signature. No exceptions!


NAME____________________________________________________

COMPANY NAME____________________________________________

ADDRESS_________________________________________________
(FedEx can only ship to street addresses - no P.O. boxes)

CITY, STATE, ZIP________________________________________

PHONE NUMBER____________________________________________
(required for shipping & tracking)


EMAIL ADDRESS___________________________________________
(Print Carefully - required in case we have a question and to
send you a confirmation that your order has been shipped)

TYPE OF CREDIT CARD:

______VISA _____MASTERCARD _____AMEX

CREDIT CARD# __________________________________________

EXPIRATION DATE________________________________________

NAME ON CARD___________________________________________

TOTAL AMOUNT (Including Shipping): $___________________

DATE:x__________________

(Required) SIGNATURE:x_________________________________
I understand that I am purchasing the Millions Vol. 7 e-mail
address CD, the addresses are not rented, but are mine to use for
my own mailing, over-and-over. Free bonuses are included, but
cannot be considered part of the financial transaction. It is
the users responsibility to comply with any laws applicable to
their local area. As with all software, once opened the CD may
not be returned, however, if found defective it will be replaced
with like product at no charge.

You may fax your order to us at: 1-435-404-1340

CHECK BY FAX SERVICES!

Please Note: Sorry, we can only accept checks drawn on U.S.
banks.

If you would like to fax a check, tape your check below and
fax it to our office along with the EZ Order Form to:
1-435-404-1340

******************************************************

***24 HOUR FAX SERVICES*** PLEASE PASTE YOUR

CHECK HERE AND FAX IT TO US AT 1-435-404-1340

*******************************************************

If You fax a check, there is no need for you to mail the
original. We will prepare a one-time draft, with the exact
information on your original check. All checks will be
held for bank clearance. (7-10 days) Make payable to:
"CD-Marketing"





























********************************************************
Do not reply to this message -
********************************************************
To register your e-mail address for future removal, visit:
http://www.OptList.com/register.html.
********************************************************



From daemon Tue Jan 18 07:43:45 2000



From: Stephen Griffiths :      s.griffiths-at-ucl.ac.uk
Date: Tue, 18 Jan 2000 12:59:53 -0000
Subject: LM: Large slides for large sections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Everyone

One of our Ph.D students has to mount some large sections of flattened
visual cortex.
The sections are about 10 to 12 cm square.

I cannot find any supplier of microscope slides, that big, in our
catalogues.

Does anyone know of a supplier/manufacturer of large glass slides?
They must exist, surely?
UK supplier preferred, but anyone, anywhere if necessary.

TIA

Stephen
{} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {}
Stephen Griffiths
Visual Science Department
Institute of Ophthalmology
Bath Street, London. EC1V 9EL
e-mail:- s.griffiths-at-ucl.ac.uk (work)
or stephen.griffiths-at-dial.pipex.com (home)
{} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {}




From daemon Tue Jan 18 18:53:24 2000



From: Evans Stephen SJ :      Stephen.Evans-at-aguk.zeneca.com
Date: Tue, 18 Jan 2000 14:58:48 -0000
Subject: LM: Efficient clearing of Arabidopsis seed coats

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hello!

Not having a lot of microscopy experience, I was wondering if anyone could
recommend some solutions for effectively clearing Arabidopsis seed coats. I
would like to use as non-toxic a solution as possible (no Xylene!) and also
one which works quickly. Can anyone give me some handy hints?

Thanks for you help!

Stephen Evans
Wheat Improvement Centre
Norwich Research Park
Colney
Norwich NR4 7UH
stephen.evans-at-aguk.zeneca.com




From daemon Thu Jan 20 07:35:25 2000



From: Michael Coviello :      coviello-at-mae.uta.edu
Date: Tue, 18 Jan 2000 10:40:29 -0600
Subject: Getting back to you

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Roy:
Sorry I haven't gotten back with you sooner, but I was off for the
holidays. Do you still need equipment? Give me a call so we can
discuss what you need.
Regrards,
Mike Coviello
817 272-5496



From daemon Tue Jan 18 18:53:27 2000



From: Marisa Ahmad :      mahmad-at-semiconductor.com
Date: Tue, 18 Jan 2000 12:34:22 -0500
Subject: re: pump lifetimes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I received this question from a co-worker. Please respond directly to me.

thanks in advance,
Marisa Ahmad

--------------------

Small question. We have a Leybold mechanical pump hooked to a Reactive Ion
Etcher. It is running 24 hrs/ day, and pumps actively on the chamber about
35 times per day. How often should a pump under these conditions be rebuilt?
The reason I am asking is the pump seems to be blowing seals and requires
rebuilding about once a year, the manufacturer says this is normal...is it?
If not, what should we do to improve the time between rebuilds?



From daemon Wed Jan 19 08:04:47 2000



From: Dr G. R. Coulton [bs_mp] :      g.coulton-at-ic.ac.uk
Date: Wed, 19 Jan 2000 10:16:23 +0000
Subject: 11th International Congress of Histochemistry and

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Michael Davidson at Florida State University has an excellent demonstration
of the QX3 microscope at:
http://microscopy.fsu.edu

Don O'Leary
----- Original Message -----
} From: "Caroline Schooley" {schooley-at-mcn.org}
To: {Microscopy-at-Sparc5.Microscopy.Com}
Sent: Sunday, January 16, 2000 12:44 PM


11th International Congress of Histochemistry and Cytochemistry (ICHC 2000)

ON-LINE REGISTRATION NOW AVAILABLE


Dear Collegues,

I have mentioned before the upcoming meeting ICHC 2000 3-8 Sept. 2000 York, UK

There will a range of sessions that will I am sure be of great interest to
this group. Please take a look at our web-site at
http://www.med.ic.ac.uk/external/ichc_2000

Hope to see you their

Best wishes

Gary Coulton
Organiser ICHC 2000



Dr. Gary Coulton
Molecular Pathology
Division of Biomedical Sciences
Imperial College School of Medicine
The Sir Alexander Fleming Building
South Kensington
London SW7 2AZ

tel 0044 (0)171 594 3190
fax 0044 (0)171 594 3022

e-mail g.coulton-at-ic.ac.uk

-------------------------------------
Announcing the 11th International Congress of Histochemistry and
Cytochemistry (ICHC 2000)

"Understanding Biocomplexity: The Post-Genome Challenge"

September 3-8, 2000, York, United Kingdom

ICHC 2000 will comprise 27 symposia addressing the latest developments and
applications of histochemistry and cytochemistry in the life sciences
including medicine.

Many leading experts to speak

On-line registration now open!!!!!!!!!!

For further details of the meeting and how to pre-register please visit our
web-site at http://www.med.ic.ac.uk/external/ichc_2000

Hope to see you there.



From daemon Wed Jan 19 19:14:41 2000



From: john grazul :      grazul-at-physics.bell-labs.com
Date: Wed, 19 Jan 2000 10:02:34 -0500
Subject: Need Sony printer drivers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




john grazul wrote:

} Fellow Microscopists,
}
} I "found" a Sony Mavigraph UP-D7000 Digital Color Printer with 2 cases
} of consumables. What I could not find is any of the software to run the
} little beast; and besides it was suppose to go on a Unix system, so
} even if I found the software it would be useless {I think}.
}
} I went to the Sony Web site and found no downloads, any other
} suggestions or even some discs etc... would be a great help. BTW, the
} Sony will be on a PC.
}
} Thanks,
}
} John Grazul
} Lucent




From daemon Wed Jan 19 19:14:46 2000



From: William Tivol :      tivol-at-wadsworth.org
Date: Wed, 19 Jan 2000 12:18:50 -0500
Subject: Martian summer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers,
I thought I'd beat Tina to the weather report. Here in
Albany, NY (where cryo-microscopy means working with the
windows open) we are having Martian summer--yesterday's
high was -15 C.
Yours,
Bill Tivol




From daemon Wed Jan 19 19:14:47 2000



From: Tamara Howard :      howard-at-cshl.org
Date: Wed, 19 Jan 2000 13:11:22 -0500 (EST)
Subject: Robert Derby search

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Sorry to bug everyone, but I'm trying to reach Robert Derby. If you are
out there, please e-mail me! Or if anyone has a contact address for him,
could you send it to me?

Thanks!

Tamara Howard
CSHL





From daemon Wed Jan 19 19:14:47 2000



From: Beth Dickey :      ecdickey-at-engr.uky.edu
Date: Wed, 19 Jan 2000 13:41:02 -0500
Subject: Job Opening

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


{fontfamily} {param} Times_New_Roman {/param} {bigger}

{/bigger} {/fontfamily} {bigger} {bold} {fontfamily} {param} Times {/param} {bigger} RESEARCH
ASSOCIATE

Electron Microscopy Facility

University of Kentucky


{/bigger} {/fontfamily} {/bold} {fontfamily} {param} Times {/param} {bigger} The
University of Kentucky invites applications for a Research Associate in
the area of electron microscopy. The Electron Microscopy Facility
houses two SEMs, two TEMs one AFM and extensive sample preparation
equipment that are used by undergraduate and graduate students in
support of research and education. The primary responsibility of the
Research Associate will be to oversee the JEOL 2010F Field Emission TEM
and auxiliary equipment, including an Oxford energy dispersive x-ray
spectrometer (EDS), Gatan electron energy loss imaging filter and
acquisition hardware and software. S/he will be responsible for
coordinating and administering maintenance, access, training and use of
the equipment by internal and external users. S/he will also be
encouraged to develop sponsored research programs and collaborate with
university faculty members. The qualified candidate will have a Ph.D.
and practical experience in analytical electron microscopy and
demonstrate good communication skills. The salary will be commensurate
with qualifications and experience. The University of Kentucky offers
comprehensive insurance and benefits packages and is an equal
opportunity employer. Please send applications to:


Professor Elizabeth Dickey

Director, Electron Microscopy Facility

University of Kentucky

A254 ASTeCC Bldg.

Lexington, KY 40506-0286

{/bigger} {/fontfamily} {/bigger}

***********

Elizabeth C. Dickey

Assistant Professor

Department of Chemical and Materials Engineering

University of Kentucky

177 Anderson Hall

Lexington, KY 40506-0046

ph: 606.257.2300 ext.288

FAX: 606.323.1929

http://www.engr.uky.edu/CME/faculty/dickey/



{/x-rich}



From daemon Wed Jan 19 19:14:48 2000



From: UIC Network Services Kit User :      netid-at-machine.cc.uic.edu
Date: Wed, 19 Jan 2000 12:43:40 -0600
Subject: frozen sections, need help

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi, I am trying to get frozen sections of rat skin about 10 um thick.
The tissue was perfusion fixed w/ 4% para in PBS then cryoprotected in
sucrose/ PBS and mounted with OTC compound. More often then not my
sections are sticking to the knife edge and are hard to remove even with
a brush. I'm a bit knew to cryo-sectioning so any tips would be greatly
appreciated.
Thanks, Andy




From daemon Wed Jan 19 19:14:48 2000



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Wed, 19 Jan 2000 09:04:38 -1000 (HST)
Subject: Re: Martian summer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} Dear Listers,
} I thought I'd beat Tina to the weather report. Here in
} Albany, NY (where cryo-microscopy means working with the
} windows open) we are having Martian summer--yesterday's
} high was -15 C.
} Yours,
} Bill Tivol


OK, OK, it's in the 70sF, raining with enough sun for spectacular
rainbows, and there's a break in the winter North Shore surf
season. But I'm stuck in a windowless lab just like the rest of you guys!

http://wavetrak.surfline.com/pipecam.asp

My condolences on your winter blues.

Aloha,
Tina


****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************





From daemon Wed Jan 19 19:14:50 2000



From: John Bonevich :      john.bonevich-at-nist.gov
Date: Wed, 19 Jan 2000 15:38:24 -0500
Subject: Re: Need Sony printer drivers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello, I have version 1.1 of a plugin for Adobe Photoshop
(Macintosh). I can send you a copy to try. You'll be printing
"pink" images in no time.

John Bonevich


} john grazul wrote:
}
} } Fellow Microscopists,
} }
} } I "found" a Sony Mavigraph UP-D7000 Digital Color Printer with 2 cases
} } of consumables. What I could not find is any of the software to run the
} } little beast; and besides it was suppose to go on a Unix system, so
} } even if I found the software it would be useless {I think}.
} }
} } I went to the Sony Web site and found no downloads, any other
} } suggestions or even some discs etc... would be a great help. BTW, the
} } Sony will be on a PC.
} }
} } Thanks,
} }
} } John Grazul
} } Lucent


--------------------------
John Bonevich, Ph.D.
NIST, Metallurgy, Stop 8554
100 Bureau Drive
Gaithersburg, MD 20899 USA
TEL: (301) 975-5428
FAX: (301) 975-4553



From daemon Wed Jan 19 19:14:52 2000



From: Caroline Schooley :      schooley-at-mcn.org
Date: Wed, 19 Jan 2000 13:42:19 -0800 (PST)
Subject: Mattel QX3 "toy" microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Please don't consider this a "commercial" posting; my goal is to encourage
lots of experimentation. The Mattel microscope
(http://www.microscopy-uk.org.uk/mag/artjan00/jhqx3.html )lists for $100,
but Toys-R-Us is now selling it for $69.95, in both its retail stores and
website. And if you go to www.etoys.com, you can download a Mattel $30
rebate certificate, good till 4/29. So it looks like you can get one for
~$40. Have fun, folks!

Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/PCI/pci.html





From daemon Wed Jan 19 19:14:54 2000



From: Walck. Scott D. :      walck-at-ppg.com
Date: Wed, 19 Jan 2000 19:43:21 -0500
Subject: Anti-vibration platform for EM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have a Micro-g anti-vibration platform that our JEOL 1200EX TEM was
sitting on until recently. This allowed us to do decent microscopy on a
vibration prone second floor with very good results. We no longer have a
need for this unit. It could be used with a TEM or SEM.

What we would like to do is trade it for a new Mac. We can not buy Macs.
If you need a platform, let's talk trade. I think that this would be a good
deal.

-Scott


Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)





From daemon Wed Jan 19 23:19:53 2000



From: Kristen Lennon :      kalen-at-citrus.ucr.edu
Date: Wed, 19 Jan 2000 17:29:23 -0800
Subject: Freeze Fracture retirement sale

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All,
We have a Balzars 301 Freeze Fracture with quartz thin film monitor, double
replica accessory, and electron guns for both platinum and carbon up for
grabs. New seals on the mechanical pump. Original equipment bought circa
1977. Last used 1998, and was perfectly fine. Free, as is. Recipient will
pay for moving.
Kristen

Kristen A. Lennon
Cell, Molecular & Developmental Biology Group
Department of Botany & Plant Sciences
University of California
Riverside, CA 92521
kalen-at-citrus.ucr.edu



From daemon Wed Jan 19 23:30:53 2000



From: Rosemary Walsh :      rw9-at-psu.edu
Date: Wed, 19 Jan 2000 23:20:22 -0600
Subject: confocal vs deconvolution microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers,
We're investigating both confocal microscopy and epi-fluorescence
combined
with de-convolution software for a multi-user facility. I'm requesting
input from directors and managers of other shared technology laboratories
regarding systems chosen and whether the system has met their expectations.
Please include websites which include FAQs and clarification of terms.
Thanks in advance for your input.
Rosemary Walsh
EM Facility for the Life Sciences
Life Science Consortium & Biotechnology Institute
Penn State University
University Park, PA. 16802
(814) 865-0212





From daemon Thu Jan 20 07:25:21 2000



From: pogany-at-power.szfki.kfki.hu (Pogany Lajos)
Date: Thu, 20 Jan 2000 10:06:12 +0100
Subject: Mattel QX3 driver

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear ALL,
there is a lot of good news about the QX3 microscope (toy-)attachment, and I
would like to try it under Windows95 or Windows NT4. Is there any driver for
this systems?
any thanks
Lajos Pogany




From daemon Thu Jan 20 07:25:22 2000



From: Corneliu Sarbu :      Corneliu.Sarbu-at-mtm.kuleuven.ac.be
Date: Thu, 20 Jan 2000 10:33:03 +0100
Subject: steel precipitates analysis by EELS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear fellow microscopists,

I have the task to analyse (by EELS technique) precipitates in steel.
I would greatly appreciate some hints coming from people who are
already familiar with the difficulties connected with:
1. carbon analysis. It is obvius that a carbon film support for the
precipitates extracted from the steel is to be avoided. Has anyone
experince on the use of other kind of supporting film
(silicium monoxide ?, beryllium ?). I have the same problem with
using copper grids, because the precipitates are supposed to
contain copper too. What kind of grids is most appropriate?

2. would it be a better solution to perform precipitate analysis on
thinned steel specimens ? Can it occurr an annoying interference
originating in the material surrounding the precipitate ?

3. last but not least, I would greatly appreciate some bibliographical
hints on that very specific topics.

Thank you in advance.

Corneliu Sarbu
MTM Dept. of KULeuven, Belgium



From daemon Thu Jan 20 18:25:28 2000



From: Donald Delaney :      delaneyd-at-mcw.edu
Date: Thu, 20 Jan 2000 08:25:58 -0600
Subject: 2 questions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello List

I have two questions to ask the List

1) Does anyone have a used TEm ultrathin microtome for sale and if so, how
much?

2) Does anyone know refernces or protocols for both immunogold labelling
and microwave embedding?





From daemon Thu Jan 20 18:25:28 2000



From: Dan Kremser :      dkremser-at-levee.wustl.edu
Date: Thu, 20 Jan 2000 08:50:19 -0600
Subject: Biological Standards for Microprobe Work

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greetings:

Recently, an interesting medical school project has appeared at my lab
door. I've been asked to quantify Ca and P in scar tissue found in
sections of hamster hearts. Obtaining appropriate standards is critical.
Are there commercially available biological standards or procedures to
create such standards out there? Any suggestions would be appreciated and
thanks for your time.

Dan



=====================================================
Dan Kremser
Washington University
Department of Earth and Planetary Sciences/Campus Box 1169
(Wilson Hall, Room 108-----for packages)
One Brookings Dr.
St. Louis, MOÊ 63130-4899

VOICE: (314) 935-5605Ê FAX: (314) 935-7361
E-MAIL: dkremser-at-levee.wustl.edu



From daemon Thu Jan 20 18:25:36 2000



From: Anita Garg :      Anita.Garg-at-lerc.nasa.gov
Date: Thu, 20 Jan 2000 10:51:15 -0500
Subject: Re: glue for cross-section

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Colleagues
What is the best glue for making cross-section samples to study ~ 30
micron reaction layer formed by a coating on a Ni-base superalloy?
The M-bond 610 which used to work great for Si and ceramic samples
does not seem to work here. Though the glue I have is pretty old.
TIA
Anita



From daemon Thu Jan 20 18:25:38 2000



From: Sobocinski, Gregg :      Gregg.Sobocinski-at-WL.com
Date: Thu, 20 Jan 2000 11:32:10 -0500
Subject: Need Recommendations for Digital Image Database/organization Soft

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Anyone have any recommendations for organizing digital images and associated
data in electronic form in a GLP environment? I'm going crazy trying to find
something that will be easy to use, will link the image with associated data
information, and will have restriction or audit capabilities to track any
changes to the data that may be made.

Any input is greatly appreciated.

Gregg Sobocinski
Parke-Davis Pharmaceuticals
Ann Arbor, Michigan
USA
Gregg.Sobocinski-at-wl.com





From daemon Thu Jan 20 18:25:38 2000



From: John F. Mansfield :      jfmjfm-at-engin.umich.edu
Date: Thu, 20 Jan 2000 12:00:18 -0500
Subject: Re: Mattel QX3 driver

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} What about the Mac? :-)
} ----------------------------------------------------------------------
} --
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




John Mansfield PhD CPhys MInstP
North Campus Electron Microbeam Analysis Laboratory
417 SRB, University of Michigan
2455 Hayward, Ann Arbor MI 48109-2143
Phone: (734) 936-3352 FAX (734) 763-2282 Cellular Phone: (734) 358-7555
(Leaving a phone message at 936-3352 is preferable to 358-7555)
Email: jfmjfm-at-engin.umich.edu
URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html
Location: Lat. 42¡ 16' 48" Long. 83¡ 43' 48"



From daemon Thu Jan 20 18:25:45 2000



From: Hendrik O. Colijn :      colijn.1-at-osu.edu
Date: Thu, 20 Jan 2000 12:54:27 -0500
Subject: MAT: making silicon oxide layers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all,

I need to make some ~100nm thick oxide layers on silicon to align an ion
gun in an auger system. I assume that most people use thermal oxidation to
grow the films.

Does anyone have a recipe for making these films? (time, temperature,
atmosphere) It would be really great if the recipe allowed me to get an
exact thickness too!

Thanks,
Henk

Hendrik O. Colijn colijn.1-at-osu.edu
Campus Electron Optics Facility Ohio State University
(614) 292-0674 http://web.ceof.ohio-state.edu
An optimist believes that we live in the best of all possible worlds.
A pessimist fears that this is true.




From daemon Thu Jan 20 18:25:40 2000



From: anderron-at-us.ibm.com
Date: Thu, 20 Jan 2000 13:17:14 -0500
Subject: Re: glue for cross-section

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


According to the data sheet packed with M-Bond 610, the room temp pot life,
after mixing, is six weeks.

We note that it fails quickly, i.e. it works fine one day and doesn't the
next. As we can't abide samples coming unglued, we toss mixed M-Bond 610
after 30 days and mix fresh.

Also, M-Bond 610 works best bonding smooth surfaces. If we have rough
surfaces we use GATAN G-1 (Epo-Tec 353ND).

Ron



Ron Anderson, IBM, Hopewell Jct., New York, USA. anderron-at-us.ibm.com

IBM Analytical Services; http://www.chips.ibm.com/services/asg



Anita Garg {Anita.Garg-at-lerc.nasa.gov} on 01/20/2000 10:51:15 AM

To: Microscopy-at-sparc5.microscopy.com
cc:


Dear Colleagues
What is the best glue for making cross-section samples to study ~ 30
micron reaction layer formed by a coating on a Ni-base superalloy?
The M-bond 610 which used to work great for Si and ceramic samples
does not seem to work here. Though the glue I have is pretty old.
TIA
Anita







From daemon Thu Jan 20 18:25:40 2000



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Thu, 20 Jan 2000 08:27:24 -1000 (HST)
Subject: Re: Mattel QX3 driver

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


}
} } What about the Mac? :-)

Remember - this thing was developed by Intel!


Aloha,
Tina

It's raining cats and dogs, and it's cold and miserable. Feel better,
now?

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************





From daemon Thu Jan 20 18:25:42 2000



From: MICHAEL MOHN :      MMOHN-at-mail.monroe.cc.mi.us
Date: Thu, 20 Jan 2000 14:24:07 -0500
Subject: LM-Bausch & Lomb Research II Metallograph

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does anyone have technical manuals or documentation for a B&L Research II Metallograph which I could copy. MCCC just received one as a donation.
Thanks!

Michael Mohn
Assistant Professor of Materials Technology
Monroe County Community College
Phone: 734-384-4122 Fax: 734-242-9711
http://www.monroe.cc.mi.us/mmohn




From daemon Thu Jan 20 18:25:46 2000



From: Leona Cohen-Gould :      lcgould-at-mail.med.cornell.edu
Date: Thu, 20 Jan 2000 17:51:29 -0400
Subject: Re: Mattel QX3 driver

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



}
} Aloha,
} Tina
}
} It's raining cats and dogs, and it's cold and miserable. Feel better,
} now?
}
} ****************************************************************************
} * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
} * Biological Electron Microscope Facility * (808) 956-6251 *
} * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
} ****************************************************************************

But what you call cold and what we call cold are two very different
animals! Its been snowing all day in the Northeast, and the temps haven't
nosed above (or even near) freezing) in days!

Lee

Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Confocal Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175





From daemon Thu Jan 20 18:25:47 2000



From: Anthony Garratt-Reed :      tonygr-at-mit.edu
Date: Thu, 20 Jan 2000 17:08:23 -0500
Subject: Re: steel precipitates analysis by EELS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I attempted to do this many years ago. I made replicas using aluminium. This
was written up in the proceedings of a conference on microanalysis
("Measurement
of carbon in V(C,N) precipitates extracted from HSLA steels in aluminum
replicas," by A.J. Garratt-Reed, in "Quantitative Microanalysis with High
Spatial Resolution," The Metals Society, London, Book No. 277, 1981, p. 165.)
Then someone else tried to replicate my work (for the moment I can't remember
his name, but he was at Strathclyde University in Glasgow) and could not get
consistent results. He concluded that the aluminum was somehow catalysing the
oxidation of the carbon in the carbides. I expect he published the results,
but I can't now remember where. I have some memory that he used silicon
monoxide to make replicas, but when I tried that, the replicas would break up
because of beam-induced charging. Of course, it would have defeated the point
to have put a carbon coat on the films!

When copper has been an issue, I have often used nickel, which are very
satisfactory. You can also get grids of many other materials, including Ti,
Al, Au, Mo, etc., etc. - check your EM suppies catalogues.

Tony G-R.




At 10:33 AM 01/20/2000 +0100, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

** Anthony J. Garratt-Reed
** MIT Room # 13-1027
** 77 Massachusetts Avenue
** Cambridge, MA 02139-4307
** USA
**
** Phone: (+) 1-617-253-4622
** Fax: (+) 1-617-258-6479
**





From daemon Thu Jan 20 18:25:47 2000



From: Walck. Scott D. :      walck-at-ppg.com
Date: Thu, 20 Jan 2000 17:44:19 -0500
Subject: RE: making silicon oxide layers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Henk,
Ta is usually used for measuring sputter rates and you can see where the
beam was put. You electrochemically grow a specific thickness and measure
the sputter rate. If you want to pursue this, I can give you a referral to
a couple of surface scientists who have done this. They are in your neck of
the woods.

Another thing that you might want to try is something that I wrote up a
number of years ago in JVST as a shop note for aligning an ion gun system
where I could not see the beam. Take Double sticky tape and put it on your
sample holder. It works in a UHV system well enough, trust me. Then push
the sample holder into yellow WO3 powder to cover the sticky tape. Where
the beam hits the sample, the WO3 will turn blue. A couple of sample
transfers and you have it.

-Scott


Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)


"The opinions expressed are those of Scott D. Walck and not of PPG
Industries, Inc. nor of any PPG-associated companies."
--


} -----Original Message-----
} From: Hendrik O. Colijn [mailto:colijn.1-at-osu.edu]
} Sent: Thursday, January 20, 2000 12:54 PM
} To: Microscopy-at-Sparc5.Microscopy.Com
} Subject: MAT: making silicon oxide layers
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} ---------.
}
}
} Hi all,
}
} I need to make some ~100nm thick oxide layers on silicon to
} align an ion
} gun in an auger system. I assume that most people use
} thermal oxidation to
} grow the films.
}
} Does anyone have a recipe for making these films? (time,
} temperature,
} atmosphere) It would be really great if the recipe allowed
} me to get an
} exact thickness too!
}
} Thanks,
} Henk
}
} Hendrik O. Colijn colijn.1-at-osu.edu
} Campus Electron Optics Facility Ohio State University
} (614) 292-0674 http://web.ceof.ohio-state.edu
} An optimist believes that we live in the best of all possible worlds.
} A pessimist fears that this is true.
}
}



From daemon Thu Jan 20 19:45:45 2000



From: Walck. Scott D. :      walck-at-ppg.com
Date: Thu, 20 Jan 2000 18:40:25 -0500
Subject: RE: steel precipitates analysis by EELS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I read Tony's answer to your question and I can't add anything to that.
However, I just have a minor point. If you are using EELS, why are you
worried about the grids? The grids would not show up in the EELS spectrum.
Of course it would interfere if you are using EDS.

You can get your grids in almost any material you want nowadays. Ni, Mo,
Be, Al, Cu, C, etc. Just pick one that doesn't interfere with your EDS
spectrum.

-Scott


Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)


"The opinions expressed are those of Scott D. Walck and not of PPG
Industries, Inc. nor of any PPG-associated companies."
--



} -----Original Message-----
} From: Corneliu Sarbu [mailto:Corneliu.Sarbu-at-mtm.kuleuven.ac.be]
} Sent: Thursday, January 20, 2000 4:33 AM
} To: microscopy-at-Sparc5.Microscopy.Com
} Subject: steel precipitates analysis by EELS
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.} html
}
}
} --------------------------------------------------------------
} ---------.
}
}
} Dear fellow microscopists,
}
} I have the task to analyse (by EELS technique) precipitates in steel.
} I would greatly appreciate some hints coming from people who are
} already familiar with the difficulties connected with:
} 1. carbon analysis. It is obvius that a carbon film support for the
} precipitates extracted from the steel is to be avoided. Has anyone
} experince on the use of other kind of supporting film
} (silicium monoxide ?, beryllium ?). I have the same problem with
} using copper grids, because the precipitates are supposed to
} contain copper too. What kind of grids is most appropriate?
}
} 2. would it be a better solution to perform precipitate analysis on
} thinned steel specimens ? Can it occurr an annoying interference
} originating in the material surrounding the precipitate ?
}
} 3. last but not least, I would greatly appreciate some bibliographical
} hints on that very specific topics.
}
} Thank you in advance.
}
} Corneliu Sarbu
} MTM Dept. of KULeuven, Belgium
}



From daemon Thu Jan 20 18:41:07 2000



From: Gaener Rodger :      grodger-at-molbiol.ox.ac.uk
Date: Thu, 20 Jan 2000 18:32:55 -0600
Subject: information about current TEM and Confocal technologies

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html





Dear users,

I am celating information about current TEM and Confocal technologies with
the intention of setting up both a confocal suite and an EM suite at a new
facility. However, I am new to this field and would like some advice on
the pros. and cons. of different systems based on other user experiences,
to give me an idea of what I should be looking out for. Specifically, the
confocal suite would allow the study of both live (EGFP) and fixed
samples. Thus, the type of confocal needed should have good resolution
for both live and fixed samples as well as good phase contrast.
Importantly, this will probably be a multi-user facility, which needs
reliable lasers that do not need to be realigned often. It would also be
important to have good quality microscope, filters and objectives as well
as extras such as a heated stage, video and CCD cameras, appropriate
computer workstations and software. Any advice that you could offer on
such equipment would be very much appreciated.

Thanking you in advance,


Dr Gaener Rodger.

-------------------------------------------------------------------

Dr Gaener Rodger
Sir William Dunn School of Pathology
University of Oxford
South Parks Road
Oxford
UK.





From daemon Thu Jan 20 19:15:46 2000



From: Nestor J. Zaluzec :      zaluzec-at-Sparc5.Microscopy.Com
Date: Thu, 20 Jan 2000 19:08:26 -0600
Subject: Microscopy Society of America - Council Election Results

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Colleagues

The results of the recent election of officers to the Society are now official
The current list of elected officers is below.



The new officers elected this year are:

President-Elect
RON ANDERSON

Secretary
JANET H. WOODWARD (2000-2002)

Director, Physical Sciences
THOMAS F. KELLY (2000-2002)

Director, Biological Sciences
SARA E. MILLER (2000-2002)

-------------------------------------------
The complete list of officers is given below
and on the MSA WWW site
http://www.msa.microscopy.com/MSADocs/MSAOfficers.html
--------------------------------------------



MSA COUNCIL 2000

President
KEN DOWNING
326 Donner Lab
Lawrence Berkeley Lab
Berkeley, CA 94720
(510) 486-5941; Fax (510) 486-6488
Email: khdowning-at-lbl.gov

President-Elect
RON ANDERSON
IBM Analytical Services
IBM Zip-41E
Hopewell Junction, NY 12533
(914) 892-2225; Fax (914) 892-2555
E-mail: ron-anderson-at-vnet.ibm.com

Past-President
DAVID JOY
Rm.232 Science and Engineering Research Facility
Univ. of Tennessee
Knoxville, TN 37996-0810
(865) 974-3642, Fax (865) 974-9449
and
Rm. S189, High Temperature Materials Laboratory
Oak Ridge National Laboratory
Oak Ridge, TN 37831-6064
(865) 574-6799
E-mail: djoy-at- utk.edu

Secretary
JANET H. WOODWARD (2000-2002)
Buckman Laboratories, Inc.
1256 N. McLean Blvd.
Memphis, TN 38108-1241
(901) 272-6408; Fax (901) 272-6451
E-mail: jhwoodward-at-buckman.com

Treasurer
KATHI ALEXANDER (1999-2001)
Los Alamos National Lab
MST-8 G755 P. O. Box 1663
Los Alamos, NM 87545
(505) 665-4750, Fax (505) 667-8021
Email: kbalexander-at-lanl.gov

Directors, Physical Sciences

J. MURRAY GIBSON (1998-2000)
Argonne National Laboratory
Materials Science Division
Argonne, Ill 60439 {p}
Email:gibson-at-anl.gov

THOMAS F. KELLY (2000-2002)
2021 Chamberlain Ave.
Madison, WI 53705-4076
(608) 263-1073; Fax (608) 262-8353
E-mail: tfkelly-at-engr.wisc.edu

MIKE KERSKER (1999-2001)
JEOL USA
11 Dearborn Rd
Peabody MA 01960
(508) 535-5900 Fax (508) 536-2205
E-mail: kersker-at-jeol.com

Directors, Biological Sciences
SARA E. MILLER (2000-2002)
Duke Medical Center, Pathology
Box 3712
Durham, NC 27710
(919) 684-3452; Fax (919) 684-8735
E-mail: saram-at-acpub.duke.edu

AVRIL SOMLYO (1998-2000)
Dept. of Molecular Physiol. & Bio. Phys.
Box 10011, Health Sciences Center
Univ. of Virginia
Charlottesville, VA 22906-0011
(804) 982-0825; Fax (804) 982-1616
E-mail: avs5u-at-virginia.edu

JOHN BOZZOLA (1999-2001)
EM Ctr - Mailcode 4402
Southern Illinois Univ
Carbondale IL 62901
(618) 453-3730, Fax (618) 453-2665
E-mail: bozzola-at-siu.edu

Director, Local Affiliated Societies
EV OSTEN (2000-2002)
3M Company
3M Center, Bldg. 201-BE-16
St. Paul, MN 55144-1000
(651) 736-0104; Fax (651) 733-0648
E-mail: efosten-at-mmm.com


==============================================
Nestor
Your Friendly Neighborhood SysOp






From daemon Fri Jan 21 07:34:51 2000



From: Debbie Nieuwenhuis :      watersci-at-sauk.com
Date: Thu, 20 Jan 2000 20:01:02 -0600
Subject: reticle

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I was wondering if anyone could tell me how to properly install a reticle in
an Olympus microscope eyepiece. The eyepiece housing does not seem to come
apart, although there are two tiny round holes on either site of the lens.
Is there a special tool needed?

Thanks!

Deb




From daemon Fri Jan 21 10:20:51 2000



From: Anthony Garratt-Reed :      tonygr-at-mit.edu
Date: Fri, 21 Jan 2000 10:55:08 -0500
Subject: RE: steel precipitates analysis by EELS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have to admit that until I read Scott's posting, I had missed the point
about the elemental interference from the grid not being a problem with
EELS analysis.

However, in the case of copper, there is another effect to worry about when
making replicas, if the process involves any sort of flotation on, or
picking the sample up from, water. Because of its electronegativity,
copper can dissolve in the water and then ion-exchange with other elements
from the sample, replacing them. Then you really do have copper in the
sample. The problem isn't particularly severe with extraction replicas
from steels (but then, I have never used extraction replicas from steels to
try to analyze for small amounts of copper in the precipitates), but has
been terrible, for example, when looking at iron sulphides, which actually
had a visible shell of copper sulphide (but I was also fishing those
samples out of brine, not distilled water!). The problem doesn't seem to
occur with nickel grids, which I use in preference to copper for this type
of application, just to be on the safe side.

BTW, I'm glad you got my posting, Scott - I haven't seen it come back to
myself yet. I guess the poor mail redirector gets indigestion with the
volume it has to deal with!

Tony.



** Anthony J. Garratt-Reed
** MIT Room # 13-1027
** 77 Massachusetts Avenue
** Cambridge, MA 02139-4307
** USA
**
** Phone: (+) 1-617-253-4622
** Fax: (+) 1-617-258-6479
**





From daemon Fri Jan 21 18:19:15 2000



From: Robert Derby :      rjderby-at-excite.com
Date: Fri, 21 Jan 2000 08:22:55 -0800 (PST)
Subject: Me

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


************************************************
Robert J. Derby
New Mexico Institute of Technology
Socorro, N.M.
Phone - 505-835-5866
E-mail - rjderby-at-excite.com
************************************************
I have heard through the grapevine that Tamara Howard from Cold Spring
Harbor is looking for me.
I was told it was posted here but I never got it.
Tamara call me at 505-835-5866(Iam 2 hours behind you)
Robert





_______________________________________________________
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Visit http://freeworld.excite.com




From daemon Fri Jan 21 18:19:15 2000



From: Neal Leddy :      nleddy-at-tcd.ie
Date: Fri, 21 Jan 2000 16:27:58 -0000
Subject: Polymerising Epoxy Resin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Group,

Just a quick question, I was wondering if anyone had ever tried using a
microwave oven to harden their resin??? And if so what sort of results were
achieved.
Another question I have is how to remove / dissolve hardened resin from an
aluminium surface???

I would appreciate any suggestions or advice.
Thank you

Neal Leddy




From daemon Fri Jan 21 18:19:16 2000



From: Ford M. Royer :      froyer-at-bitstream.net
Date: Fri, 21 Jan 2000 10:32:09 -0600
Subject: TEM Available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Anyone interested in the following TEMs FOR SALE?

Phillips 201C
Complete system, fully operational, taken out of service in December.

JEOL model JEM-100B. - Parts Unit Only

--
Ford M. Royer, MT(ASCP)
Analytical Instruments, Ltd
(Refurbished Histology, Cytology, & General Lab Equipment)
9921 13th Ave. N.
Minneapolis, MN 55441-5004
800-565-1895 phone
612-929-1895 fax
web site: http://www.aibltd.com





From daemon Fri Jan 21 18:19:16 2000



From: Keith Ryan :      KPR-at-wpo.nerc.ac.uk
Date: Fri, 21 Jan 2000 17:19:40 +0000
Subject: glutaraldehyde safety - summary (M)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello all

Many thanks to all who responded on and off-line to my request for
information. I hope I acknowledged each one individually.

My quest was to find the real reason for lowering the exposure limit.
I won't summarise the replies but: (1) pulmonologists at one hospital
are thinking that long term, low level exposure to formaldehyde and
glutaraldehyde may cause idiopathic pulmonary fibrosis, (2) I was told
of a serious skin burn caused by a splash.

I have now heard from the UK Health & Safety Commission's Advisory
Committee on the Toxicity of Substances (ACTS). I have copy from: HSE
Review 1997, published 1999, section C58 (consisting of 4 pages).
Quotes from this are below.

The official reason for lowering the exposure limit is that it has
not been possible to set a no-observed adverse effect level for
glutaraldehyde with regard to the induction of asthma.

Carcinogenicity is not supported by the available good-quality
evidence to date.
________________________________________

Glutaraldehyde must be labelled under the Chemicals (Hazard
Information and Packaging for supply) Regulations 1994 (CHIP) as
Toxic, Corrosive, Sensitising and Dangerous for the Environment.

Several thousand tonnes are imported into the UK each year. It is
primarily used as a biocide and disinfectant in the health care,
off-shore, paper-making and agricultural sectors.

.. it is estimated that a considerable number of (health care)
workers are intermittently exposed, given the widespread use in that
sector. Similarly, ....... for several hundred workers in the
manufacture of glutaraldehyde solutions.

..... available exposure data relates mainly to use in the health
care sector....... suggests that under normal operational conditions
short term exposures are generally less than 0.2 ppm. This can be
exceeded during the cleaning of endoscopes ...... or the wiping of
surfaces.

HEALTH EFFECTS - ANIMAL STUDIES.
Glutaraldehyde is acutely toxic to rats by inhalation, oral and
dermal routes. The principal effects are due to its irritant
properties.

Glutaraldehyde is clearly a skin sensitiser in rabbits, guinea pigs,
rats and mice.

Glutaraldehyde is clearly mutagenic in vitro, in bacterial and
mammalian cells. ............. No firm conclusions can be drawn from
the available evidence on chromosomal aberrations, but glutaraldehyde
clearly causes sister chromatid exchange (SCE) and unscheduled DNA
synthesis (UDS) in mammalian cells.

......... However, the clearly negative results of recent,
good-quality bone marrow cytogenetics and peripheral blood
micronucleus tests, together with those of the liver UDS assay,
provide reassurance that the genotoxic effects shown in vitro are
unlikely to be expressed in vivo.

No reports of carcinogenicity studies of glutaraldehyde by the
inhalation or dermal routes of administration are currently available.
A recent oral study provided no convincing evidence ... in rats .....
drinking water for up to two years.

No significant effects on reproduction were reported in a modern two
generation study ........... There were no indications of significant
gonadal effects in 13 weeks inhalation studies carried out in rats or
mice, or in a lethal assay in mice.

HUMAN DATA
Glutaraldehyde is irritant to to human skin at concentrations of
2-10%, but not 0.5%. Higher concentrations have not been
investigated.

There is substantial evidence that glutaraldehyde is a skin
sensitiser in humans. Concentrations as low as 0.13% have induced
allergic contact dermatitis. The majority of cases have been reported
in health or funeral workers.

A fair body of evidence ............. indicates that glutaraldehyde
has the potential to cause occupational asthma.

.......... several workplace studies with exposure data in which no
cases of asthma have been found among contemporary workers. .....
However, superimposed on these data are other reports of sensory
irritation and / or asthma in endoscopy nurses where the reported
levels of exposure overlap with those in the above studies.
.................. From the data available, it has not been possible
to determine a NOAEL (no-observed adverse effect level) for the
induction of asthma.

No information is available in genotoxicity in humans.

The only available mortality study is of limited value, but does not
provide any evidence that glutaraldehyde caused cancer or increased
mortality in glutaraldehyde production workers.

On the basis of the very limited information available, it does not
appear that glutaraldehyde causes reproductive toxicity in humans.

REFERENCE: Glutaraldehyde: Criteria document for an occupational
exposure limit EH/65/32. HSE Books ISBN 0 7176 1443 3.
_________________________________________________

Dr. Keith Ryan
Marine Biological Association of the UK
The Laboratory
Citadel Hill
Plymouth
Devon PL1 2PB
England

Tel. ++44 (0)1752 633249
Tel. ++44 (0)1752 633279
The 279 number has an answering machine

Fax ++44 (0)1752 633102

e-mail: kpr-at-wpo.nerc.ac.uk

PS - Daniele, you're still here! Keep smiling!! That was a nice
evening in Strasbourg. Do you know which Gewurztraminer we all had as
an aperatif? Now the world will wonder?!




From daemon Fri Jan 21 18:19:21 2000



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Fri, 21 Jan 2000 14:18:24 -1000 (HST)
Subject: Images to PowerPoint

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


************************************************
Robert J. Derby
New Mexico Institute of Technology
Socorro, N.M.
Phone - 505-835-5866
E-mail - rjderby-at-excite.com
************************************************
Does anyone or any company know of a ccd that will capture single photon at
the 852-872 wavelength?
This is needed for a special project.
Any help would be of great help
Thanks,

Robert





_______________________________________________________
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Visit http://freeworld.excite.com



1256 N. McLean Blvd.
Memphis, TN 38108-1241
(901) 272-6408; Fax (901) 272-6451
E-mail: jhwoodward-at-buckman.com

Treasurer
KATHI ALEXANDER (1999-2001)
Los Alamos National Lab
MST-8 G755 P. O. Box 1663
Los Alamos, NM 87545
(505) 665-4750, Fax (505) 667-8021
Email: kbalexander-at-lanl.gov

Directors, Physical Sciences

J. MURRAY GIBSON (1998-2000)
Argonne National Laboratory
Materials Science Division
Argonne, Ill 60439 {p}
Email:gibson-at-anl.gov

THOMAS F. KELLY (2000-2002)
2021 Chamberlain Ave.
Madison, WI 53705-4076
(608) 263-1073; Fax (608) 262-8353
E-mail: tfkelly-at-engr.wisc.edu

MIKE KERSKER (1999-2001)
JEOL USA
11 Dearborn Rd
Peabody MA 01960
(508) 535-5900 Fax (508) 536-2205
E-mail: kersker-at-jeol.com

Directors, Biological Sciences
SARA E. MILLER (2000-2002)
Duke Medical Center, Pathology
Box 3712
Durham, NC 27710
(919) 684-3452; Fax (919) 684-8735
E-mail: saram-at-acpub.duke.edu

AVRIL SOMLYO (1998-2000)
Dept. of Molecular Physiol. & Bio. Phys.
Box 10011, Health Sciences Center
Univ. of Virginia
Charlottesville, VA 22906-0011
(804) 982-0825; Fax (804) 982-1616
E-mail: avs5u-at-virginia.edu

JOHN BOZZOLA (1999-2001)
EM Ctr - Mailcode 4402
Southern Illinois Univ
Carbondale IL 62901
(618) 453-3730, Fax (618) 453-2665
E-mail: bozzola-at-siu.edu

Director, Local Affiliated Societies
EV OSTEN (2000-2002)
3M Company
3M Center, Bldg. 201-BE-16
St. Paul, MN 55144-1000
(651) 736-0104; Fax (651) 733-0648
E-mail: efosten-at-mmm.com


==============================================
Nestor
Your Friendly Neighborhood SysOp





--CAB19553.948442177/styx.services.ou.edu--


} From MAILER-DAEMON Fri Jan 21 02:09 CST 2000
Received: from localhost (localhost) by Sparc5.Microscopy.Com (8.6.11/8.6.11) with internal id CAA00349; Fri, 21 Jan 2000 02:09:34 -0600


Hello, all-

I'm hoping to hear from those of you who have worked out the optimum size
and resolution to make images in e.g., Photoshop that are destined for
PowerPoint to be made into transparencies. An image that is 4 x 5 inches
and 400-600 dpi seems to be overkill for a 35mm slide.

Your opinions?

Mahalo,
Tina
****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************




From daemon Fri Jan 21 18:49:25 2000



From: David Henriks :      Henriks-at-CompuServe.COM
Date: Fri, 21 Jan 2000 18:35:08 -0600
Subject: Inconel 718 Polish

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Nick:

I found the following recipe from Bernie Kestel. He states that this is
used for surface polishing using the beaker method. Although he did not
use it for "jet polishing", it may be a good starting point.

Inconel 718 (annealed)
10% HClO4
90% Ethanol
Temperature = -60C
Current: 275mA
Volts: as required

There is some more information about stir rate, sample orientation etc.
that is only relevant for the beaker method. If you'd like to try this
approach, I would be happy to send you the entire reference.

I hope this helps.

Best regards-

David
Writing at 10:14:39 AM on 01/21/2000

***************************************************************************
************************

David Henriks
Vice President TEL: 800-728-2233
(toll free in the USA)
South Bay Technology, Inc. +1-949-492-2600
1120 Via Callejon FAX: +1-949-492-1499
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

***************************************************************************
************************

} } } } } Please visit us at http://www.southbaytech.com { { { { {

Manufacturers of precision sample preparation equipment and supplies for
metallography, crystallography and electron microscopy.

Message text written by Schryvers Dominique
}
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


I'm looking for a good electropolishing solution + conditions for
as-received and annealed Inconel 718 for use with a Tenupol 3 system to
produce well thinned matrix + precipitates for TEM work. Any suggestions?

Nick Schryvers






From daemon Sat Jan 22 11:28:25 2000



From: Larry Stoter :      LPS-at-teknesis.demon.co.uk
Date: Sat, 22 Jan 2000 09:55:54 -0600
Subject: Re: steel precipitates analysis by EELS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

A minor observation ...........

While the issue of overlaps is generally not a problem with EELS (as
opposed to EDS), if can arise with steel precipates. The vanadium L
and Oxygen K edges are really quite close, so if you have vanadium
precipatates, a silicon oxide support film will cause problems for
quantitation.

regards,
--
Larry Stoter
17, Rocks Park Road, Uckfield, E. Sussex, TN22 2AT, UK
email: LPS-at-teknesis.demon.co.uk, Home Phone/Fax: +44 (0)1825 767967





From daemon Sat Jan 22 11:28:25 2000



From: COURYHOUSE-at-aol.com
Date: Sat, 22 Jan 2000 12:19:05 EST
Subject: Re: Martian summer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Yikes! Great! set the microscope near the window and get those snow flake
pictures! It has been close to 80 degrees a few days this week here in
Arizona!
I have a great batch of infusion brewing on the back patio.

Got the happage win TV card to add to the computer....Have the c-mount ccd
camera I found for $100.... now... I should be able to share pictures soon!

Ed Sharpe
archivist for SMECC

{ { Subj: Martian summer
Date: 1/22/00 7:50:37 AM Pacific Standard Time
From: tivol-at-wadsworth.org (William Tivol)
Sender: tivol-at-wadsworth.org
To: microscopy-at-sparc5.microscopy.com

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America
To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
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-----------------------------------------------------------------------.


Dear Listers,
I thought I'd beat Tina to the weather report. Here in
Albany, NY (where cryo-microscopy means working with the
windows open) we are having Martian summer--yesterday's
high was -15 C.
Yours,
Bill Tivol
} }



From daemon Sun Jan 23 16:27:39 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Mon, 24 Jan 2000 01:16:18 -0600
Subject: Administrivia: Server Replaced this weekend

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Colleagues

The main Microscopy Server was replaced this weekend (read
that as many blurry eyed hours in front of a monitor, and lots
of cursing at new formats of configuration files for DNS and SENDMAIL).

The old beast was growing increasing unreliable with hardware
crashes nearly daily. I believe that most of the services have been
restored however until all databases are reconfigured and tested there
will be some glitches. Please be patient.

I think I have been able to capture the few messages that were sent
over the weekend. If those of you that posted items over the weekend
don't see things in the next day please repost them.

Cheers...

Nestor
Your Friendly Neighborhood SysOp




From daemon Mon Jan 24 07:52:03 2000



From: G. Macdonald :      glenmac-at-u.washington.edu
Date: Mon, 24 Jan 2000 01:26:50 -0600
Subject: Re: Images to PowerPoint

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Tina,
Our medical photography dept. requests that files submitted for
slides are at least 1 Mb and no more than 3 Mb in size. Less than this
doesn't have enough pixels to adequately fill the image (similar to a thin
negative), and more is unneeded. I create the slide figure, set to 300 dpi
and adjust dimensions to put the file size into that range.

Regards,
Glen


Glen MacDonald
Research Scientist
Hearing Research Laboratories of the
Virginia Merrill Bloedel Hearing Research Center
Box 35-7923
University of Washington
Seattle, WA 98195-7923
(206) 616-4156
glenmac-at-u.washington.edu

On Fri, 21 Jan 2000, Tina Carvalho wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hello, all-
}
} I'm hoping to hear from those of you who have worked out the optimum size
} and resolution to make images in e.g., Photoshop that are destined for
} PowerPoint to be made into transparencies. An image that is 4 x 5 inches
} and 400-600 dpi seems to be overkill for a 35mm slide.
}
} Your opinions?
}
} Mahalo,
} Tina
} ****************************************************************************
} * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
} * Biological Electron Microscope Facility * (808) 956-6251 *
} * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
} ****************************************************************************
}
}
}




From daemon Mon Jan 24 07:52:03 2000



From: Pnina Ari-Gur :      arigurp-at-wmich.edu
Date: Mon, 24 Jan 2000 01:26:54 -0600
Subject: Faculty Position Materials Science

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Position: Full/Associate/Assistant Professor of Materials Science and
Engineering. This is a full time, tenure track teaching and research
position beginning Fall 2000.

Rank and Salary: Rank will depend upon background and experience; salary
competitive.

Responsibilities: Teach undergraduate and graduate courses in materials
science and engineering, advise undergraduate and graduate students,
seek and conduct funded research, supervise theses and projects, and
serve on university, college and department committees.

Academic
Qualifications: Earned Ph.D. in materials science and/or engineering or
a closely related field is required. Demonstrated experience in research
and grant seeking is highly desired. Prior teaching experience a plus.

Experience
Qualifications: Demonstrated experience in research and grant seeking is
highly desired. Prior teaching experience a plus.

Department: The Department of Construction Engineering, Materials
Engineering and Industrial Design at Western Michigan University
currently offers two undergraduate BSE -- Materials Engineering and
Construction Engineering and Management, and BS in Industrial Design.
The Department also offers two Master of Science programs - Materials
Science and Engineering and Construction Management.

University: Western Michigan University, with a student body of
approximately 28,000 is located in southwest Michigan. Kalamazoo is
halfway between Chicago and Detroit and 45 miles south of Grand Rapids.
The population of the greater Kalamazoo area is approximately 200,000.
Its industry is highly diversified and it is the center of many cultural
and sporting events.

Applications: Review of applications will begin on January 10, 2000, and
will continue until the position is filled. Please send the following
credentials: Letter of Application addressing qualifications, Vitae,
Transcripts from all institutions, and the names/addresses,
telephone/fax numbers of three references

Contact: Please send credentials to:

Dr. Roman Rabiej, Chair
Western Michigan University
Department of Construction, Materials, & Industrial Design
2007 Kohrman Hall
Kalamazoo, MI 49008

AN EQUAL OPPORTUNITY/AFFIRMATIVE ACTION EMPLOYER
Western Michigan University is an Equal Opportunity Employer. In
addition, it has embarked upon a vigorous affirmative action program and
encourages the applications of women and members of minority groups.
==============================
Pnina Ari-Gur, D.Sc., Professor of
Materials Science and Engineering
Western Michigan University
Kalamazoo, MI 49008
(616) 387-3372 FAX: (616) 387-6517
email: pnina.ari-gur-at-wmich.edu
http://www.wmich.edu/cmd/arigur.htm
==============================




From daemon Mon Jan 24 07:52:02 2000



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Mon, 24 Jan 2000 01:26:57 -0600
Subject: PCD for JEOL 840 wanted

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Happy New Year

Does anyone have a Probe Current Detector accessory for a JEOL 840
surplus to requirements and available for purchase?
I think its designation is PCD40, it's the pneumatically-operated
thing which mounts on the column opposite to the objective aperture
holder, and shoots a Faraday cup across into the beam.

thanks

Ritchie Sims


Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand




From daemon Sun Jan 23 16:27:44 2000



From: de Lillo Enrico :      delillo-at-agr.uniba.it
Date: Mon, 24 Jan 2000 14:41:14 +0100
Subject: Re: EURAAC meeting

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Dr. Fashing,
it is a pleasure receiving news from you. I have to apologize for the
trouble you have reported in your letter and I hope to help you.

First of all, the website is going to be updated because of the second
circular is ready to be sent.

The second point is that you have enough time to submit your paper or
poster. The deadline for submission has been established on 30 April 2000.
I hope you answered to the first circular (my database has not been
recently updated - Migliorini is working on this and he has the complete
and updated archive), so in this case you will receive the second circular
directly on your computer. Otherwise I suggest you to fill the application
on the web site.

Let me know if I could help you more. I will be glad to do that.

My best wishes and see you in Siena


Enrico


} Dear Dr. De Lillo,
}
} I am interested in attending the EURACC symposium in Siena this July,
} and would greatly appreciate receiving information concerning that
} meeting. I keep checking the meeting homepage on the web site
} (http://www.unisi.it/ricerca/dip/bio_evol/sitoeuraac/siena2000.html),
} but very little information is given. I also contacted the e-mail
} address (euraac2000-at-unisi.it) a few months back and have not yet
} received a reply. I wrote to Dr. Leo P. S. van der Geest, the EURAAC
} Secretary, yesterday and he gave me your e-mail address and thought
} perhaps you could help.
}
} I would like to present a paper at the meeting (probably a poster)
} and need to know when titles and abstracts are due and to whom they
} should be sent. Also the format that should be used for submitting
} the paper.
}
} Many thanks for your help; it is greatly appreciated. I look forward
} to hearing from you.
}
} Sincerely,
}
} Norm
}
} Norm Fashing
} Professor of Biology
} Department of Biology
} College of William and Mary
} P.O. Box 8795
} Williamsburg, VA 23187-8795
} 757 221-2221 (Office)
} 757 221-6483 (FAX)
} njfash-at-facstaff.wm.edu
} http://www.wm.edu/biology/Fashing.html


dr Enrico de Lillo
Istituto di Entomologia agraria - Universitˆ Bari - Italy
via Amendola, 165/A - 70126 Bari - Italy
tel. +39 080 5443105
fax +39 080 5442876
email: delillo-at-agr.uniba.it
http://193.204.185.103/de_lillo.htm




From daemon Mon Jan 24 08:04:35 2000



From: Marti, Jordi :      jordi.marti-at-honeywell.com
Date: Mon, 24 Jan 2000 06:46:26 -0700
Subject: Reichert Ultracut E with cryo FC 4D

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The power supply in our cryo microtome is having problems which might be
related to the transformer. I was told by the service engineer that the
transformer is no longer supported by Reichert. Does any one know where I
can get a replacement ?

Thanks

Jordi Marti



From daemon Mon Jan 24 15:07:21 2000



From: Leona Cohen-Gould :      lcgould-at-mail.med.cornell.edu
Date: Mon, 24 Jan 2000 11:04:49 -0400
Subject: Re: Administrivia: Server Replaced this weekend

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Nestor,
Thanks for taking such good care of us.
Lee

Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Confocal Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175




From daemon Mon Jan 24 15:07:23 2000



From: Sobocinski, Gregg :      Gregg.Sobocinski-at-WL.com
Date: Mon, 24 Jan 2000 10:05:22 -0500
Subject: Ignore This Message (Testing)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


This is a test to see if my messages are being posted to the listserver.




From daemon Mon Jan 24 15:07:24 2000



From: Sobocinski, Gregg :      Gregg.Sobocinski-at-WL.com
Date: Mon, 24 Jan 2000 10:35:13 -0500
Subject: Need Recommendations for Digital Image Database/organization Soft

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Anyone have any recommendations for organizing digital images and associated
data in electronic form in a GLP environment? I'm going crazy trying to find
something that will be easy to use, will link the image with associated data
information, and will have restriction or audit capabilities to track any
changes to the data that may be made.

Any input is greatly appreciated.

Gregg Sobocinski
Parke-Davis Pharmaceuticals
Ann Arbor, Michigan
USA
Gregg.Sobocinski-at-wl.com



From daemon Mon Jan 24 15:07:24 2000



From: Walck. Scott D. :      walck-at-ppg.com
Date: Mon, 24 Jan 2000 10:41:36 -0500
Subject: RE: Inconel 718 polish

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Contact Robin Griffin at UAB. I bought that unit there and we worked on 718
and developed the polishing conditions for it. I believe that we used the
butyl cellusolve(SP?)/perchloric acid solution at low temp to do it. She
should have the recipe or know who to contact. Her Email address is
rgriffin-at-eng.uab.edu or you might get a hold of Ray Thompson whose student
did the work. As I recall, the as-cast material was very difficult to do
and had very narrow conditions. The annealed samples were a little easier.
To save time, we had the samples initially cut out of the bulk samples using
an EDM machine.

-Scott


Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)


"The opinions expressed are those of Scott D. Walck and not of PPG
Industries, Inc. nor of any PPG-associated companies."
--



} -----Original Message-----
} From: Schryvers Dominique [mailto:schryver-at-ruca.ua.ac.be]
} Sent: Friday, January 21, 2000 2:53 AM
} To: Microscopy MAIL
} Subject: Inconel 718 polish
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.} html
}
}
} --------------------------------------------------------------
} ---------.
}
}
} I'm looking for a good electropolishing solution + conditions for
} as-received and annealed Inconel 718 for use with a Tenupol 3
} system to
} produce well thinned matrix + precipitates for TEM work. Any
} suggestions?
}
} Nick Schryvers
}
}
} *=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*
} *=* *=*
} *=* Dr. D. Schryvers *=*
} *=* Electron Microscopy for Materials Research (EMAT) *=*
} *=* University of Antwerp, RUCA *=*
} *=* Groenenborgerlaan 171 *=*
} *=* B-2020 ANTWERP *=*
} *=* Belgium *=*
} *=* tel: 32-3-2180247 *=*
} *=* fax: 32-3-2180257 *=*
} *=* e-mail: schryver-at-ruca.ua.ac.be *=*
} *=* homepage: http://www.ruca.ua.ac.be/EMAT *=*
} *=* *=*
} *=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*
}
}



From daemon Mon Jan 24 15:07:25 2000



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Mon, 24 Jan 2000 09:45:39 -0600
Subject: Re: Images to PowerPoint

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The short answer to you question would be an image of about 1024 pixels
across should be adequate for a slide used for a presentation. The slide
will appear to most people as a 8x10"-print held at arm's length, or as a
computer screen at 4 to 6 feet. If you cannot make out the pixels in those
images, then you probably have enough pixels in your image for PowerPoint.

I think I start seeing the pixels when the resolution drops to 800 or 640
pixels across. I might see a benefit in raising the pixels to 1280 across,
but I am not able to see improvement beyond that point. This would give you
an image of about 1 million pixels.

Now if you are shooting your slide with a 35-mm camera, it might be good to
use 24-bit color on the image if your output device (e.g., printer) can
well render it. However, since the slide is only for a presentation, you
can probably get by with much less color depth if file size per slide is an
issue. I venture to say that 8-bit color at 1024 pixels across is plenty
adequate for most presentations.

Remember, the above considerations are only for images for slide
presentations. If the slides are meant to archive the images for other
purposes, then you probably want every bit of resolution and color depth
that your technology allows and justifies.

Warren

At 02:18 PM 1/21/2000 -1000, you wrote:
} Hello, all-
}
} I'm hoping to hear from those of you who have worked out the optimum size
} and resolution to make images in e.g., Photoshop that are destined for
} PowerPoint to be made into transparencies. An image that is 4 x 5 inches
} and 400-600 dpi seems to be overkill for a 35mm slide.
}
} Your opinions?
}
} Mahalo,
} Tina



From daemon Mon Jan 24 15:07:34 2000



From: Frank Thomas :      thomasf-at-AGC.BIO.NS.CA
Date: Mon, 24 Jan 2000 14:24:57 -0400
Subject: just a test

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Just checking to see if I'm still "wired to the world".....


F.C. Thomas
MicroAnalysis Facility
Geological Survey of Canada (Atlantic)
Bedford Institute of Oceanography
Dartmouth, Nova Scotia
Canada B2Y 4A2



From daemon Mon Jan 24 15:07:34 2000



From: Greg Erdos :      gwe-at-biotech.ufl.edu
Date: Mon, 24 Jan 2000 13:33:28 -0500
Subject: Bio Cryo

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Periodically questions appear on this list concerning the use of cryostats
for cutting histological sections. I usually reply to the sender off line
and offer a copy of a handout that I got from a workshop at a Histochem
Meeting some years ago. Even though it is old, cryostat sectioning has not
changed a lot. I think there is some valuable info there, especially for
beginners. I thought it might be helpful to make this available on the net
for whomsoever might want to take a look.
It can be found at :
http://www.biotech.ufl.edu/sems/

Look for the snowflake

It was written by Bruce Quinn, then of MIT. I hope he has no objections
to my posting it.

Greg Erdos
Gregory W. Erdos, Ph.D. Ph. 352-392-1295
Assistant Director, Biotechnology Program
PO Box 110580 Fax:
352-846-0251
University of Florida
Gainesville, FL 32611





From daemon Mon Jan 24 15:07:34 2000



From: David H. Hall :      hall-at-aecom.yu.edu
Date: Mon, 24 Jan 2000 13:51:22 -0500
Subject: EM technician position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


TECHNICAL POSITION AVAILABLE

A full time position is available immediately for a highly motivated
individual to work in the Center for C. elegans Anatomy at the Albert
Einstein College of Medicine, located in the Bronx, New York. The
candidate should have a Bachelor's degree in Biology or some related
science, and some previous laboratory experience. We are particularly
looking for an individual with training in transmission electron microscopy
and thin section microtomy. Experience with immunocytochemistry and/or
computerized image analysis is helpful but not required.

The College offers a generous compensation package including 4 weeks
vacation and tuition reimbursement. Qualified candidates should submit a
resume and a list of references to:

Dr. David Hall
Department of Neuroscience
Albert Eistein College of Medicine
1410 Pelham Parkway
Bronx, NY 10461

email: hall-at-aecom.yu.edu
FAX: 718 430-8821
phone: 718 430-2195



From daemon Mon Jan 24 15:07:36 2000



From: Cieslinski, Robert (RC) :      RCCIESLINSKI-at-dow.com
Date: Mon, 24 Jan 2000 13:33:07 -0600
Subject: uProbe - Position Available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Position Available:

Electron Microprobe Analyst

Company: The Dow Chemical Company

Location: Midland, Michigan

Qualifications (education, certification, language, etc.) and Experience
required:
A candidate with a BS or MS or PHD degree in physical science is preferred.
Prior experience in electron probe microanalysis is essential.


Job Overview:
The Surface/Microscopy/X-ray (SMX) Group in Dow's Corporate R&D Analytical
Science Laboratory has one professional level full time opening for an
electron microprobe analyst. The candidate should have theoretical and
practical experience in electron beam techniques, including quantitative
x-ray microanalysis, digital imaging, digital x-ray imaging, electron
beam/solid interactions, scanning electron microscopy and material science.
Good computer skills are very desirable. Good written and oral
communication skills and the ability to work both independently and in a
team environment are extremely important.


Key responsibilities will include:
1. Extensive problem solving on a wide variety of Dow materials and
processes
2. Operation and routine maintenance of a CAMECA SX-50 electron microprobe.
3. Some sample preparation including microtomy and metallography
4. Operation of light microscopes.
5. Operation of scanning and transmission microscopes as needed.
6. Interpretation of images.
7. Documentation of work.
8. Compliance with safety and quality systems


Interested:
Please e-mail or send your resume and cover letter, with reference to this
ad to:
Email: R&D-at-Dow.com or The Dow Chemical Company, Workforce Planning, P. O.
Box 150, Plaquemine, Louisiana 70765-0150. E-mail respondents must list Job
006145 and their last name as the first and second items on the Subject
line. Only those selected for an interview will be contacted. Only U.S.
citizens or aliens who are authorized to work in the United States will be
considered for employment.

We are an equal opportunity employer and offer a competitive compensation
and benefits package including 401k, stock purchase, tuition reimbursement
and performance incentives. The Dow Chemical Company is the fifth largest
chemical company in the world with annual sales of US$20billion. Dow
manufactures and supplies chemicals, plastics and agricultural products for
customers in 164 countries and employs approx. 43,000 people worldwide. For
more news and information about Dow, please visit our web site at
www.dow.com.






Robert C. Cieslinski
The Dow Chemical Company
Microscopy & Microanalysis
(517) 636-6875
email: rccieslinski-at-dow.com



From daemon Mon Jan 24 15:07:39 2000



From: Ekstrom, Harry :      harry.ekstrom-at-honeywell.com
Date: Mon, 24 Jan 2000 13:08:38 -0700
Subject: Need Recommendations for Digital Image Database/organization

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We successfully import our digital images into a PDF file using Adobe
Acrobat.

Harry Ekstrom

-----Original Message-----
} From: Sobocinski, Gregg [mailto:Gregg.Sobocinski-at-WL.com]
Sent: Monday, January 24, 2000 8:35 AM
To: 'Microscopy-at-MSA.Microscopy.Com'


Anyone have any recommendations for organizing digital images and associated
data in electronic form in a GLP environment? I'm going crazy trying to find
something that will be easy to use, will link the image with associated data
information, and will have restriction or audit capabilities to track any
changes to the data that may be made.

Any input is greatly appreciated.

Gregg Sobocinski
Parke-Davis Pharmaceuticals
Ann Arbor, Michigan
USA
Gregg.Sobocinski-at-wl.com



From daemon Mon Jan 24 21:02:20 2000



From: Robert Derby :      rjderby-at-excite.com
Date: Mon, 24 Jan 2000 20:46:41 -0600
Subject: CCD

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does anyone or any Company know of a CCD that will do single photon
detection at 852 wavelength?
This is for a very specialized app.
Thanks in advance




************************************************
Robert J. Derby
New Mexico Institute of Technology
Socorro, N.M.
Phone - 505-835-5866
E-mail - rjderby-at-excite.com
************************************************





_______________________________________________________
Get 100% FREE Internet Access powered by Excite
Visit http://freeworld.excite.com




From daemon Tue Jan 25 07:33:19 2000



From: Michael Reiner :      michael.reiner-at-Smail.Uni-Koeln.de
Date: Tue, 25 Jan 2000 07:52:03 +0100
Subject: EM: Shelf life of LR-Gold

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear members of the list,

first, I would like to wish you all the best for the new year.
Now my question:
Does anyone know the shelf-life/usability of LR-Gold stored in a fridge?

Is it that delicate as LR-White (Meanwhile I don«t use LR-W older than
half a year). My bottle which was not opened many times, could be
roundabout three years old.

Thanks a lot,
Michael

Michael Reiner
Department of Anatomy I
University of Cologne
Germany
michael.reiner-at-smail.uni-koeln.de



From daemon Tue Jan 25 18:19:12 2000



From: Schryvers Dominique :      schryver-at-ruca.ua.ac.be
Date: Tue, 25 Jan 00 15:50:33 +0100
Subject: glass: TEM sample prep

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Dear all,

we're starting a project of HRTEM on small (nanoscale) colouring metal
particles in glass. Does anyone have experience with sample preparation
for this type of material. We prefer ion-milling as this retains the
relative positions of the particles with respect to the surface. Any
suggestions, also on literature, are welcome.

Many thanks in advance,

Nick Schryvers




*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*
*=* *=*
*=* Dr. D. Schryvers *=*
*=* Electron Microscopy for Materials Research (EMAT) *=*
*=* University of Antwerp, RUCA *=*
*=* Groenenborgerlaan 171 *=*
*=* B-2020 ANTWERP *=*
*=* Belgium *=*
*=* tel: 32-3-2180247 *=*
*=* fax: 32-3-2180257 *=*
*=* e-mail: schryver-at-ruca.ua.ac.be *=*
*=* homepage: http://www.ruca.ua.ac.be/EMAT *=*
*=* *=*
*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*



From daemon Tue Jan 25 18:19:12 2000



From: Greg Erdos :      gwe-at-biotech.ufl.edu
Date: Tue, 25 Jan 2000 09:50:51 -0500
Subject: Re: cryo bio

Contents Retrieved from Microscopy Listserver Archives
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by ultra5.microscopy.com (8.9.1b+Sun/8.9.1) id IAA06203
for dist-Microscopy; Tue, 25 Jan 2000 08:53:13 -0600 (CST)
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for "MicroscopyFilteredEmail-at-msa.microscopy.com"; Tue, 25 Jan 2000 08:52:42 -0600 (CST)
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Received: from empc1 by snarl.biotech.ufl.edu (8.8.5/4.09)
id JAA02445; Tue, 25 Jan 2000 09:47:10 -0500 (EST)
Message-Id: {4.2.0.58.20000125094650.00a545f0-at-biotech}
X-Sender: gwe-at-biotech
X-Mailer: QUALCOMM Windows Eudora Pro Version 4.2.0.58


Some people have trouble and some do not. It is an Acrobat . After you
get the blank screen, try hitting the reload button on your browser
menu. This has worked for some people. I need to consult a web expert to
see why my PDF files cause trouble.

Nestor, are you out there?????

If you still have trouble, let me know and I will put it into an HTML file.
My apologies to anyone having trouble.

Greg Erdos


At 09:39 AM 01/25/2000 -0500, you wrote:
} Dear Greg;
} I was most interested to look at your tips etc. for cryo sectioning,
} but when I clicked on the snowflake, all I ended up with was a blank
} screen. Any idea what I did wrong (or is my computer system to blame?)
}
} thanks in advance
} shea
}
}
}
} Dr. S. Shea Miller
} Agriculture and Agri-Food Canada
} Eastern Cereal and Oilseed Research Centre
} 2068 K.W. Neatby Bldg
} Central Experimental Farm
} Ottawa, Ontario
} Canada K1A 0C6
} email: millers-at-em.agr.ca
} phone: 613-759-1760
} fax: 613-759-1701

Gregory W. Erdos, Ph.D. Ph. 352-392-1295
Assistant Director, Biotechnology Program
PO Box 110580 Fax:
352-846-0251
University of Florida
Gainesville, FL 32611





From daemon Tue Jan 25 18:19:29 2000



From: McCaffrey, John :      John.McCaffrey-at-nrc.ca
Date: Tue, 25 Jan 2000 11:42:08 -0500
Subject: glass: TEM sample prep

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Dominique,

Your best source of advice would be Scott Walck, at these contacts:

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)

Scott has been in the 'TEM of glass' business for years and has developed a
series of techniques relevant to the preparation of cross-sections of this
material, which I assume you require when you say, "we prefer ion-milling as
this retains the
relative positions of the particles with respect to the surface." As Scott
will probably tell you, there is a small-angle cleaving technique that you
may find preferable to ion milling.

Cheers
John

John P. McCaffrey
National Research Council of Canada
M-50, Montreal Rd.
Ottawa, Ontario
K1A 0R6 CANADA

tel: +613-993-7823
fax: +613-990-0202
email: john.mccaffrey-at-nrc.ca


-----Original Message-----
} From: Schryvers Dominique [mailto:schryver-at-ruca.ua.ac.be]
Sent: Tuesday, January 25, 2000 9:51 AM
To: Microscopy MAIL


Dear all,

we're starting a project of HRTEM on small (nanoscale) colouring metal
particles in glass. Does anyone have experience with sample preparation
for this type of material. We prefer ion-milling as this retains the
relative positions of the particles with respect to the surface. Any
suggestions, also on literature, are welcome.

Many thanks in advance,

Nick Schryvers




*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*
*=* *=*
*=* Dr. D. Schryvers *=*
*=* Electron Microscopy for Materials Research (EMAT) *=*
*=* University of Antwerp, RUCA *=*
*=* Groenenborgerlaan 171 *=*
*=* B-2020 ANTWERP *=*
*=* Belgium *=*
*=* tel: 32-3-2180247 *=*
*=* fax: 32-3-2180257 *=*
*=* e-mail: schryver-at-ruca.ua.ac.be *=*
*=* homepage: http://www.ruca.ua.ac.be/EMAT *=*
*=* *=*
*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*



From daemon Tue Jan 25 18:19:59 2000



From: Yali Tang :      ytang-at-ameslab.gov
Date: Tue, 25 Jan 2000 17:53:48 +0100
Subject: BG by OM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html





Dear all,

we're starting a project of patterning on BG of YBCO films. I got
difficulties to find the BG by using opital microscope, because we
can not etch the sample before patterning. Does anyone have
experience with checking the GB by OM? We appraciate any suggestions
or references.

Thanks in advance,

Yali Tang
--



From daemon Tue Jan 25 18:19:36 2000



From: Mark Aindow :      maindow-at-ims.uconn.edu
Date: Tue, 25 Jan 2000 12:14:09 -0500
Subject: Postdoc Position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


University of Connecticut
Institute for Materials Science

Postdoctoral Research Position in Electron Microscopy

The Institute for Materials Science (IMS) at UConn is an
interdisciplinary center with the threefold mission of fostering
education, research and outreach in all areas of the materials
sciences. Due to a re-organization of the IMS Microscopy
Unit, a Postdoctoral Position has become available in the area
of transmission electron microscopy. The appointee will be
involved in a range of academic and industrial projects, and
will assist in developing the TEM facilities. Candidates should
hold a PhD in Materials Science, Physics or a related discipline
and must have extensive hands-on experience in a broad range
of electron microscopy techniques. Experience in instrument
development and/or computer image processing/simulation
would be beneficial. The appointment is for one year in the
first instance and is available immediately. Screening of the
applications will begin immediately and will continue until the
post is filled. Applications from under-represented groups,
including minorities, women and people with disabilities are
encouraged.

Interested candidates should send a curriculum vitae, including
publication list, and the names of at least three referees with
postal addresses, telephone numbers and Email addresses to:
Prof. M. Aindow, Institute for Materials Science, University of
Connecticut, 97 North Eagleville Road, U-3136, Storrs,
CT 06269-3136 USA. Email: maindow-at-ims.uconn.edu




From daemon Tue Jan 25 18:19:41 2000



From: Neilly,Joseph :      joe.p.neilly-at-abbott.com
Date: Tue, 25 Jan 2000 13:20:09 -0600
Subject: Mattel QX3 on the Mac

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I recently asked Michael Davidson of FSU (http://microscopy.fsu.edu/) if he
had any success running the QX3 on a Mac w/ USB and he said "It works with a
windows 98 emulator, but very very slowly."

Several people have had success running the QX3 with twain drivers from other
programs such as Paint Shop Pro and Photoshop
(http://clubs.yahoo.com/clubs/qx3). Does anybody know of a twain driver that
would run the QX3 from a Mac?

Joe Neilly
MMMS Educational Outreach Chair
voice: (847)-938-5024
fax: (847)-938-5027
e-mail: joe.neilly-at-abbott.com



From daemon Tue Jan 25 18:19:42 2000



From: Mitch McCartney :      Mitch.McCartney-at-alconlabs.com
Date: Tue, 25 Jan 2000 13:30:47 -0600
Subject: EM Position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Position Title: (Technical-Level) Scientist-Electron Microscopy

Company: Alcon Laboratories, Inc., a wholly owned subsidiary of Nestle,
S.A., has 10,000 employees and worldwide sales of $2.4 billion. It is the
global leader in the discovery, development, manufacture and marketing of
ophthalmic pharmaceuticals and medical devices. Alcon expects to double its
sales over the next five years and has achieved a profit growth rate of
approximately 11% over the last several years. Historically, the company
commits 10% of sales to research and development. Products developed in the
last ten years generate 50% of current sales. The current product pipeline
is strong. Alcon was just renamed to the Fortune List of the 100 Best
Companies to Work for in America.

Location: Fort Worth was recognized by USA Today as one of the 20 best
cities in which to live and work.

Position Responsibilities: The EM Unit is a core resource for R&D, witnessed
by the fact that the staff of three generated 9,350 electron micrographs
from 1,160 processed specimens in 1998 alone. The successful candidate will
be a key member of the EM Unit who processes, examines and provides
preliminary interpretation of ophthalmic devices as well as human and animal
tissue specimens from a wide variety of R&D groups. S/he will provide
electron microscopic research and method development directed towards the
discovery of new drug candidates and unique ophthalmic devices, the
understanding of pathogenic mechanisms and the identification of new
therapeutic agents. S/he will handle the EM Unit commitment to several
groups: Surgical-Intraocular Lens, Toxicology, and Degenerative Diseases, as
well as contributing to Glaucoma Therapeutic Research, Surgical,
Formulations, Consumer Technical Support, Physical Characterization and
Pharmacokinetics.

Responsibilities include preparing ophthalmic devices for SEM and x-ray
analysis and human and animal tissue for TEM and SEM; use and daily
maintenance of Zeiss CEM-902, Cambridge Stereoscan-120 and Zeiss DSM-940,
and PGT System 4+ x-ray system; developing and implementing EM techniques
for various research projects; assisting with human and animal tissue
procedures; and providing preliminary interpretation of EM data.

Preferred Qualifications: Candidates should possess a Bachelor of Science
degree in a related discipline plus at least seven years of significant EM
experience related exclusively to human and animal tissue. Collaborative
and problem solving skills are essential for this position. The successful
candidate will also demonstrate highly refined interpersonal and technical
writing skills. Certification by or eligibility to be certified by the MSA
is a plus.

Alcon professionals enjoy state-of-the-art facilities in a year-round
business casual environment. Our company offers competitive salaries and a
wide array of excellent benefits: a very generous retirement plan and
dollar-for-dollar matching (up to 5%) 401K, medical, dental, vision, life,
and accident insurance, death, dismemberment, illness and disability
benefits, tuition reimbursement, employee credit union, adoption assistance,
dependent care, and wellness programs, on-site fitness center, running
track, cafeteria, and company store, innovative paid time off and holidays,
and retiree medical coverage.

An Equal Opportunity and Affirmative Action Employer. Pre-employment drug
testing.

Please email your resume and salary requirements to:
Job28_1261-at-careers.alconlabs.com
Reference Code: EM




From daemon Tue Jan 25 18:19:43 2000



From: Mandayam V. Parthasarathy :      mvp2-at-cornell.edu
Date: Tue, 25 Jan 2000 14:43:14 -0500
Subject: Subject: Bio Cryo/pdf file

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Apparently several were unable to read the cryostat technique pdf file that
Dr. Greg Erdos had generously posted at his website. The answer to the
problem could be the version of Acrobat used. I had the "blank page"
problem with version 3.0 but no problem at all with version 4.0 (Mac).



*******************************************************************

M.V. Parthasarathy
Prof. of Plant Biology, Adjunct Prof. of Biomedical Sciences (Vet), &
Director, Cornell Integrated Microscopy Center (CIMC)
Section of Plant Biology
228 Plant Science Building
Cornell University, Ithaca, NY 14853
E-Mail: mvp2-at-cornell.edu
Plant Biology Office: 268 Emerson; Telephone: 607-255-1734
Plant Biology Fax: 607-255-5407
CIMC Office: C1 054 Vet. Med. Center; Telephone: 607-253-3803
CIMC Office Fax: 607-253-3803
CIMC web site: http://www.cimc.cornell.edu




From daemon Tue Jan 25 18:19:46 2000



From: wft03-at-health.state.ny.us
Date: Tue, 25 Jan 2000 15:14:36 -0500
Subject: Cold

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html






}
} Aloha,
} Tina
}
} It's raining cats and dogs, and it's cold and miserable. Feel better,
} now?
}

But what you call cold and what we call cold are two very different
animals! Its been snowing all day in the Northeast, and the temps haven't
nosed above (or even near) freezing) in days!


Dear Lee,
Fair is fair. Often wind and rain at slightly above freezing is more
miserable
than snow at a few degrees below--having been both in San Francisco for the
first
condition and Albany NY for the second, I can say this with first-hand
authority.
Yours,
Bill Tivol




From daemon Tue Jan 25 18:19:49 2000



From: wft03-at-health.state.ny.us
Date: Tue, 25 Jan 2000 16:03:37 -0500
Subject: Repair/overhaul of a Kevex Detector

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Appropriate vendors,
I have a Kevex detector which has been giving peak widths
larger than specs. It probably just needs an overhaul, but there may
be some repair necessary for the pre-amp and FET. Could anyone
who can undertake this please respond to me off-list with estimates
for various contingencies? TIA.
Yours,
Bill Tivol




From daemon Tue Jan 25 18:19:54 2000



From: Ronald C. Decker :      decker-at-utcdayton.com
Date: Tue, 25 Jan 2000 17:42:35 -0500
Subject: TEM & Aerospace lubrication - Researcher Needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all,

Our Ohio company is seeking an engineer / scientist to research the
relationships between the chemistry and microstructure of solid lubricant
and hard coatings and their performance in the lubrication of aerospace
systems. Research will involve a variety of surface analytical tools (XPS,
Raman, etc.) so that fundamental mechanisms of lubrication can be
elucidated. Emphasis on microstructure will require expertise with TEM and
SEM including preparation of SEM & TEM specimens of thin films and wear
scars on steel and ceramic substrates. Research will also involve
correlating thin film properties with deposition plasma characteristics and
making recommendations for improving lifetime and performance of such
materials in different environments: e.g., vacuum, moist air, high
temperature, etc.

It is important that candidates have capabilities in cross-section TEM,
analytical TEM, analysis of unique microstructures; and understand TEM of
thin films on a fundamental level. It is desirable that the candidate have
knowledge of tribological materials and experience with TEM/XTEM of wear
tracks.

Contact Ronald Decker - mailto:decker-at-utcdayton.com

{} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {}
Ronald C. Decker
Program Manager
Universal Technology Corporation
1270 N FAIRFIELD RD
DAYTON OH 45432-2600

Voice (937) 426-8530, Fax (937) 426-7753
(Voice mail is available at my extension, 270)

http://www.utcdayton.com/
mailto:decker-at-utcdayton.com
{} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {}




From daemon Tue Jan 25 18:19:54 2000



From: Pete :      pjpns-at-worldnet.att
Date: Tue, 25 Jan 2000 17:51:25 -0500
Subject: Spectrophotometer Schematics

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Schematics for Carl Zeiss Spectrophotometer DM4, DMR21, PMQII being discarded. Please
respond by 1/31/00 if interested.

Pete Dondl
pjpns-at-worldnet.att.net



From daemon Tue Jan 25 18:19:58 2000



From: G. Macdonald :      glenmac-at-u.washington.edu
Date: Tue, 25 Jan 2000 15:28:42 -0800 (PST)
Subject: Re: Mattel QX3 on the Mac

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


While browsing news from the latest MacWorld Expo I came across a brief
comment about a USB video microscope for the Mac. Further searches at the
MacWorld Expo website or at Apple's web site have not turned up anything
more about it, although there were announcements that Data Translation and
National instruments
have released additional PCI and USB I/O boards for the Mac. Has anyone
heard more of this?


Regards,
Glen


Glen MacDonald
Research Scientist
Hearing Research Laboratories of the
Virginia Merrill Bloedel Hearing Research Center
Box 35-7923
University of Washington
Seattle, WA 98195-7923
(206) 616-4156
glenmac-at-u.washington.edu





From daemon Tue Jan 25 19:30:11 2000



From: Snow, David B. :      snowdb-at-pweh.com
Date: Tue, 25 Jan 2000 19:17:31 -0600
Subject: Job Opening:High Resolution Scanning Electron

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




High Resolution Scanning Electron Microscopist/Engineer


United Technologies Research Center is seeking an engineer to fill the
HR-SEM operator/engineer position at the United Technologies Research
Center in East Hartford, CT. This position will provide support to the
United Technologies Corporation Business Unites including Pratt & Whitney,
Carrier, Sikorsky, Hamilton Sundstrand and Otis. The SEM operator/engineer
will be responsible for the full utilization of both high-resolution
secondary and back-scattered imaging to characterize a wide range of
metallic and non-metallic materials, including surface coatings and
advanced structural materials including metals and ceramics. In addition,
the ability to recognize fracture modes and origins of fractures is
strongly desired. The candidate should be experienced in the use of EDS
for both qualitative and quantitative analyses, including compositional
mapping and line profiles. The qualified candidate must be capable of
judging the optimal combinations of imaging and EDS to yield t!
he most informative characterization of a particular specimen. Good
communication and interpersonal skills are essential. Experience with
electron backscatter diffraction (EBSD) is a plus.

Qualified candidates will have BS in Materials Science or an equivalent
discipline, with a minimum of 2 years SEM experience. U.S. citizenship or
permanent resident status is required.

Please visit our web site at http://www.utrc.utc.com for additional general
information. Interested parties should send a letter of application and a
resume to Employment Opportunities, Code MATS-2050-9049, United
Technologies Research Center, 411 Silver Lane, East Hartford, CT 06108 or
e-mail employment-at-utrc.utc.com. United Technologies Research Center is an
equal opportunity employer.




From daemon Wed Jan 26 08:08:20 2000



From: Damian :      dneuberger-at-mindspring.com
Date: Tue, 25 Jan 2000 20:13:24 -0600
Subject: Re: Subject: Bio Cryo/pdf file

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I tried to open the file with Acrobat 4.0 in Windows 98. No luck.

Damian Neuberger
etc., etc.



} Apparently several were unable to read the cryostat technique pdf file that
} Dr. Greg Erdos had generously posted at his website. The answer to the
} problem could be the version of Acrobat used. I had the "blank page"
} problem with version 3.0 but no problem at all with version 4.0 (Mac).



From daemon Wed Jan 26 08:08:25 2000



From: Bill Carmichael :      billc-at-jvlnet.com
Date: Tue, 25 Jan 2000 21:58:42 -0600
Subject: ISI SS40 parts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all,

We have an ISI SS40 SEM that is in need of a discontinued part. It is a
NEC transistor, number D588. It is used in the filament current
control circuit. I'm having trouble locating the part because it has
not been manufactured by NEC since 1984. Does anyone know of a source
for this part or a substitute transistor?

Thanks in advance,

Bill Carmichael

______________________________________
Bill Carmichael
Electron Microscopy Faculty
Madison Area Technical College
3550 Anderson St.
Madison, WI 53704
608-243-4309
wcarmichael-at-madison.tec.wi.us

http://electron-microscopy.madison.tec.wi.us




From daemon Wed Jan 26 08:08:25 2000



From: phil.swab-at-depsci.com (Phil Swab)
Date: Tue, 25 Jan 2000 20:11:00 -0800
Subject: glass: TEM sample prep

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dominique,
Glass is readily microtomed with a diamond knife and may be a suitable
inexpensive technique for you to consider. Particularly if the materials
of your sample are sufficiently dissimilar in reaction to the chemical and
ion etching of some techniques. I've been embedding and sectioning coated
glass, optics, and other hard materials for 18 years (even diamond coated
silicon in Microscopy Research and Technique, vol. 31, p. 308, 1995). The
imaging and analysis of nano-structures in micron sized areas near the
surface of glass is routine, fast, and inexpensive for physical
microstructure and chemistry. Mechanical artifacts generated in
ultramicrotomy tend to be quite large, readily visible, and easily ignored
but may interfere with the analysis of naturally occurring deformation
features (i.e. twinning, slip, etc.). Any good diamond knife will work
with meticulous and careful technique, but experience has shown that 35
degree knives yield the best results with hard and ultra-hard materials.

The critical elements for microtomy of hard, non-porous materials include:
1. Minimize the cross-sectional area to be sectioned. An easy way is to do
this is to pop concoidal micro-chips from the surface. These tend to be
very thin at the edges and may be further broken to form very pointed thin
samples. [Time = ~20 minutes]
2. Optimize sample orientation for sectioning and preferred orientation.
Some physical microstructures are anisotropic and are difficult to
interpret when viewed in the wrong orientation. [Time = ~10 minutes]
3. Maximize adhesion to the resin through the selection of an appropriate
resin (low viscosity and non-reactive with your sample), meticulous and
contamination-free sample prep, and the addition of adhesion promoters
(such as Dow Corning Z-6040). [Time = ~1 hour, with an over night epoxy
cure]
4. Section using standard procedures, but minimize the sectioning speed
(optimize cutting speed). [Time = ~1 hour]

These times are approximate for 1 sample, and there could be economy in
numbers. As always, each case will require individual attention.
Cheers,

Phil Swab
Engineering Development
Deposition Sciences Inc.
Santa Rosa, CA
707-566-3718
phil.swab-at-depsci.com

-----Original Message-----
} From: Schryvers Dominique [SMTP:schryver-at-ruca.ua.ac.be]
Sent: Tuesday, January 25, 2000 6:51 AM
To: Microscopy MAIL


Dear all,

we're starting a project of HRTEM on small (nanoscale) colouring metal
particles in glass. Does anyone have experience with sample preparation
for this type of material. We prefer ion-milling as this retains the
relative positions of the particles with respect to the surface. Any
suggestions, also on literature, are welcome.

Many thanks in advance,

Nick Schryvers




*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*
*=* *=*
*=* Dr. D. Schryvers *=*
*=* Electron Microscopy for Materials Research (EMAT) *=*
*=* University of Antwerp, RUCA *=*
*=* Groenenborgerlaan 171 *=*
*=* B-2020 ANTWERP *=*
*=* Belgium *=*
*=* tel: 32-3-2180247 *=*
*=* fax: 32-3-2180257 *=*
*=* e-mail: schryver-at-ruca.ua.ac.be *=*
*=* homepage: http://www.ruca.ua.ac.be/EMAT *=*
*=* *=*
*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*





From daemon Wed Jan 26 08:08:57 2000



From: Petra Wahlbring :      wahlbrin-at-crpgl.lu
Date: Wed, 26 Jan 2000 09:42:35 +0100
Subject: Re: glass: TEM sample prep

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Nick,

we are analyzing frequently this kind of specimens. In our case the
particles are silver. Depending on density and size distribution they are
changing the color of the glass.
Since we are interested in the depth distribution starting at the interface
with a silver containging layer on the glass, we need to do a
cross-sectional preparation. Therefore, we use several steps of preparation
as described below:

á gluing of two coated glass surfaces face to face

á cutting and polishing of the obtained block to a size of 2 x 1 x 10 mmÒ
with the interface plane running parallel to the 2 mm x 10 mm face and in
the middle of the block

á gluing of this block in a steel-cylinder of 3 mm diameter and 10 mm
length with a 1 mm x 2 mm rectangular hole along the cylinder axis

á cutting of 0.5 - 1 mm thick slices perpendicular to the cylinder axis

á fine polishing of one face of the obtained disk (lowest grain size: 1µm)

á grinding of the disk from the opposite face down to 100 µm thickness with
subsequent fine polishing

á dimpling of a crater into one face of the disk with a residual thickness
in the middle of the disk of about 10 µm

á ion etching of the flat side of the sample with argon ions of 4 kV under
an angle of 2 - 4 degrees with a current of about 12 µA until electron
transparency is reached in the region of the interface.

Some of the results were presented on the FEMMS-Meeting in Irsee, Germany,
1998.
For more details, you might contact me directly.

Hope this helps,

Petra

At 15:50 25.01.00 +0100, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

--------------------------------------------------------------
Dr. Petra Wahlbring
Centre de Recherche Public - Gabriel Lippmann
Laboratoire d'Analyse des Materiaux (LAM)
162a, av. de la Faiencerie L-1511 Luxembourg
tel. +352-466644-402 fax +352-466644-400
e-mail: petra.wahlbring-at-crpgl.lu
Visit our WWW site! http://www.crpgl.lu/~wahlbrin



From daemon Wed Jan 26 08:28:08 2000



From: Hyman, S.C. :      sch10-at-leicester.ac.uk
Date: Wed, 26 Jan 2000 14:00:54 -0000
Subject: SEM - Halophilic bacteria preparation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Can anybody offer a method for the preparation of Halophilic bacteria for
'standard' SEM observation? post fixation washing appears to produce cell
lysis!.



From daemon Wed Jan 26 08:28:09 2000



From: Greg Erdos :      gwe-at-biotech.ufl.edu
Date: Wed, 26 Jan 2000 09:19:58 -0500
Subject: Cryostat info.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


For those who are unable to view the PDF file of Cryostat information that
I posted, I have also posted it in ugly HTML. I am still trying to find
out why some can read the PDF and others cannot. The Version of Acrobat
does not seem to be the answer.

My apologies to anyone who got frustrated.
Once again the site is:
www.biotech.ufl.edu/sems/

Greg Erdos
Gregory W. Erdos, Ph.D. Ph. 352-392-1295
Assistant Director, Biotechnology Program
PO Box 110580 Fax:
352-846-0251
University of Florida
Gainesville, FL 32611





From daemon Wed Jan 26 09:29:37 2000



From: Ann-Fook Yang (Ann-Fook Yang) :      yanga-at-em.agr.ca
Date: Wed, 26 Jan 2000 09:39:51 -0500
Subject: No weather report please

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Please keep weather reports private.



} } } {"wft03-at-health.state.ny.us"-at-sparc5.Microscopy.Com} 01/25 3:14 PM } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America






}
} Aloha,
} Tina
}
} It's raining cats and dogs, and it's cold and miserable. Feel better,
} now?
}

But what you call cold and what we call cold are two very different
animals! Its been snowing all day in the Northeast, and the temps haven't
nosed above (or even near) freezing) in days!


Dear Lee,
Fair is fair. Often wind and rain at slightly above freezing is more
miserable
than snow at a few degrees below--having been both in San Francisco for the
first
condition and Albany NY for the second, I can say this with first-hand
authority.
Yours,
Bill Tivol






From daemon Wed Jan 26 09:29:37 2000



From: Ann-Fook Yang (Ann-Fook Yang) (Ann-Fook Yang) :      yanga-at-em.agr.ca
Date: Wed, 26 Jan 2000 09:43:30 -0500
Subject: No weather report please

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Please keep weather reports private.

Ann Fook



} } } {"wft03-at-health.state.ny.us"-at-sparc5.Microscopy.Com} 01/25 3:14 PM } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America






}
} Aloha,
} Tina
}
} It's raining cats and dogs, and it's cold and miserable. Feel better,
} now?
}

But what you call cold and what we call cold are two very different
animals! Its been snowing all day in the Northeast, and the temps haven't
nosed above (or even near) freezing) in days!


Dear Lee,
Fair is fair. Often wind and rain at slightly above freezing is more
miserable
than snow at a few degrees below--having been both in San Francisco for the
first
condition and Albany NY for the second, I can say this with first-hand
authority.
Yours,
Bill Tivol






From daemon Wed Jan 26 19:00:50 2000



From: Peter Guthrie :      Peter.Guthrie-at-hsc.utah.edu
Date: Wed, 26 Jan 2000 09:28:03 -0700 (MST)
Subject: USB or firewire cameras

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


It would be convenient to be able to bring a video camera into an
elementary school classroom, hook it up to a microscope (with an eyepiece
adaptor) and display the microscope image for an entire classroom to see.
If there is a video monitor (or a TV with a video input), this is fairly
easy. If there is not an available monitor, I should be able to bring in a
laptop and display the image on the laptop screen.

I am looking for an inexpensive lightweight video camera (with a C-mount)
with either a firewire or USB linkage.

Does anyone have any suggestions?

Thanks

Peter Guthrie
Department of Neurobiology & Anatomy
University of Utah School of Medicine
50 N Medical Drive
Salt Lake City, UT 84132
(801) 581-8336 (801) 581-4233 fax
Peter.Guthrie-at-hsc.utah.edu




From daemon Wed Jan 26 19:00:51 2000



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Wed, 26 Jan 2000 08:39:59 -0800
Subject: Re: Repair/overhaul of a Kevex Detector

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


DearBill,
When my Kevex detector showed degraded resolution, I turned off the bias and
grounded the BNC plug with a paper clip, then warmed it up completely to get
rid of ice and frost in the detector. This brought back my resolution, but
degraded my LN2 holding time. Then I had a friend in Physics pump out the
dewar and now I'm back to peak performance.
04:03 PM 1/25/00 -0500, you wrote:
} Appropriate vendors,
} I have a Kevex detector which has been giving peak widths
} larger than specs. It probably just needs an overhaul, but there may
} be some repair necessary for the pre-amp and FET. Could anyone
} who can undertake this please respond to me off-list with estimates
} for various contingencies? TIA.
} Yours,
} Bill Tivol
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Wed Jan 26 19:00:53 2000



From: Dan Kremser :      dkremser-at-levee.wustl.edu
Date: Wed, 26 Jan 2000 11:16:41 -0600
Subject: Biological Standards for Microprobe Work

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greetings all:

Recently, an interesting medical school project has appeared at my lab
door. I've been asked to quantify Ca and P in scar tissue found in
sections of hamster hearts. Obtaining appropriate standards is critical.
Are there commercially available biological standards or procedures to
create such standards out there? Any suggestions would be appreciated and
thanks for your time.

Dan


=====================================================
Dan Kremser
Washington University
Department of Earth and Planetary Sciences/Campus Box 1169
(Wilson Hall, Room 108-----for packages)
One Brookings Dr.
St. Louis, MOÊ 63130-4899

VOICE: (314) 935-5605Ê FAX: (314) 935-7361
E-MAIL: dkremser-at-levee.wustl.edu



From daemon Wed Jan 26 19:00:55 2000



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Wed, 26 Jan 2000 11:44:14 -0600
Subject: Re: USB or firewire cameras

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


How about using a device like Dazzle or Snappy to display the video stream
from your video camera on a laptop? It would require a little software
setup but should work. The models I am familiar with used parallel port
connections, but there ought to be models around that would use USB.

At 09:28 AM 1/26/2000 -0700, you wrote:
} It would be convenient to be able to bring a video camera into an
} elementary school classroom, hook it up to a microscope (with an eyepiece
} adaptor) and display the microscope image for an entire classroom to see.
} If there is a video monitor (or a TV with a video input), this is fairly
} easy. If there is not an available monitor, I should be able to bring in a
} laptop and display the image on the laptop screen.
}
} I am looking for an inexpensive lightweight video camera (with a C-mount)
} with either a firewire or USB linkage.
}
} Does anyone have any suggestions?
}
} Thanks



From daemon Wed Jan 26 19:00:54 2000



From: hank adams :      hpadams-at-bcm.tmc.edu
Date: Wed, 26 Jan 2000 13:38:41 -0600
Subject: sperm fixation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Listservers,
Can anyone lead me to a good reference for processing human sperm for TEM? Or if you have a procedure can you please forward the details.
TIA
Hank Adams
Integrated Microscopy Core
Molecular and Cell Biology
Baylor College of Medicine
Houston, TX



From daemon Wed Jan 26 19:01:19 2000



From: A. K. Christensen :      akc-at-umich.edu
Date: Wed, 26 Jan 2000 17:15:30 -0500
Subject: Re: sperm fixation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hank,

Here are three good references:

Stefanini M, De Martino C, Zamboni L. 1967. Fixation of ejaculated
spermatozoa for electron microscopy. Nature 216:173-174.

Meschede D, Keck C, Zander M, Cooper TG, Yeung CH, Nieschlag E. 1993.
Influence of three different preparation techniques on the results of human
sperm morphology analysis. Int J Androl 16:362-369.

Phillips DM. 1995. Fixation of mammalian spermatozoa for electron
microscopy. In: Dentler W, Witman G (Eds.), Methods in Cell Biology, Vol
47, vol 47. Academic Press Inc (San Diego), pp 199-204.

Kent

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
A. Kent Christensen, Professor Emeritus
Department of Cell and Developmental Biology, Medical Science II Building
University of Michigan Medical School, Ann Arbor, MI 48109-0616
Office: 2703A Medical Science II Building
Tel (work) (734) 763-1287, Fax (work) (734) 763-1166
E-mail: akc-at-umich.edu
http://www.umich.edu/~akc/
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

--On Wed, Jan 26, 2000 1:38 PM -0600 hank adams {hpadams-at-bcm.tmc.edu} wrote:

} Listservers,
} Can anyone lead me to a good reference for processing human sperm for
} TEM? Or if you have a procedure can you please forward the details. TIA
} Hank Adams
} Integrated Microscopy Core
} Molecular and Cell Biology
} Baylor College of Medicine
} Houston, TX




From daemon Wed Jan 26 19:01:19 2000



From: White, Woody N :      Woody.N.White-at-mcdermott.com
Date: Wed, 26 Jan 2000 17:13:00 -0600
Subject: Re:USB or firewire cameras

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Peter,

If you intend to use a "video" camera, you may need a capture card rather
than,
or in addition to, a USB or Firewire interface. Most video cams are analog
(NTSC in the US, SECAM or PAL elsewhere in the world). Others can certainly
have digital circuits internal (like the new digital camcorders or the
PCcams
used for video conferencing on the net). ...You first need to determine
the
source format.

Warren's suggestion implements an inexpensive external NTSC* video frame
grabber
(Snappy). Which will "grab" an analog video frame and digitize it. *May do
PAL
too??

Parallel port I/O is a bit slow (or is that a byte slow :) for pictures
containing lots of data, but is cheap and works since the inherent
resolution of
typical NTSC video is { 640x480. USB is very much faster and Firewire
faster
yet (and usually a lot more $$ for the interface card). For applications
other
than full frame rate/resolution streaming video, USB is fine.

Woody White



From daemon Wed Jan 26 19:16:54 2000



From: Lawrence Kordon :      nikon-at-jagunet.com
Date: Wed, 26 Jan 2000 20:04:33 -0500
Subject: Re: USB or firewire cameras

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Peter,

Take a look at this site....

http://www.dazzlemultimedia.com/html/products/index.htm

They have a USB version and the software interface is great. It is easy to use
and has a street price of about $180!

Good Luck,

Lawrence Kordon
Nikon, Inc.
Columbia, Maryland
nikon-at-jagunet.com

***I have no affiliation with Dazzle, Inc.***


"White, Woody N" wrote:

} ------------------------------------------------------------------------
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} -----------------------------------------------------------------------.
}
} Peter,
}
} If you intend to use a "video" camera, you may need a capture card rather
} than,
} or in addition to, a USB or Firewire interface. Most video cams are analog
} (NTSC in the US, SECAM or PAL elsewhere in the world). Others can certainly
} have digital circuits internal (like the new digital camcorders or the
} PCcams
} used for video conferencing on the net). ...You first need to determine
} the
} source format.
}
} Warren's suggestion implements an inexpensive external NTSC* video frame
} grabber
} (Snappy). Which will "grab" an analog video frame and digitize it. *May do
} PAL
} too??
}
} Parallel port I/O is a bit slow (or is that a byte slow :) for pictures
} containing lots of data, but is cheap and works since the inherent
} resolution of
} typical NTSC video is { 640x480. USB is very much faster and Firewire
} faster
} yet (and usually a lot more $$ for the interface card). For applications
} other
} than full frame rate/resolution streaming video, USB is fine.
}
} Woody White



From daemon Wed Jan 26 23:12:23 2000



From: Paul Webster :      pwebster-at-hei.org
Date: 26 Jan 00 17:52:26 -0800
Subject: Re:No weather report please

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} Please keep weather reports private.
}
} Ann Fook

Why? A little light heartedness never hurt anyone.
For the record, LA was sunny as usual today. Happy I don't live in CT anymore.

Regards,

Paul Webster, Ph.D.
Associate Scientist & Director
Ahmanson Advanced Electron Microscopy & Imaging Center
House Ear Institute
2100 West Third St.
Los Angeles, CA 90057

Phone: (213) 273-8026
Fax: (213) 413-6739
e-mail: pwebster-at-hei.org
http://www.hei.org/htm/aemi.htm



From daemon Wed Jan 26 23:12:28 2000



From: Lou Ann Miller :      lamiller-at-ux1.cso.uiuc.edu
Date: Wed, 26 Jan 2000 20:39:59 -0600
Subject: Re: Firewire Video & no need for capture board

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I currently video with a Sony DV Video camera, fire wire connect to
my computer with no capture board.

It does require special software however, We bought Final Cut Pro,
But from trying out the demo and reading, It appears Adobe Priemier
also will allow firewire capture without a board. Though Adobe's is
one I have not tried, I'd call first.

This works fine for video, and I can pull off individual frames for
low res images for the web, and ok small images to print if very
small.

But, in emailing to and from Sony, It was my understanding that if I
were to firewire images, I WOULD need a board.

My video camera will do both images and video. FinalCut Pro pulls
off the video, but I can't seem to get it to recognize the individual
image shots. So I believe Sony, though I may just not have selected
the right buttons etc.

If pulling in video by fire wire, especially pulling it onto a
firewire hard drive ( ie VST) It is pretty close to real time video.

Lou Ann
***************************
Lou Ann Miller
Service Supervisor
Microscopic Imaging Lab
College of Vet. Medicine
University of Illinois
2001 S Lincoln Ave
Urbana,Illinois 61801
217-244-1566
lamiller-at-ux1.cso.uiuc.edu

Microscopy Home Page:
http://treefrog.cvm.uiuc.edu

Central States Microscopy Society
http://treefrog.cvm.uiuc.edu/csmms

Personal Home Page:
http://treefrog.cvm.uiuc.edu/lam



From daemon Wed Jan 26 23:12:33 2000



From: jim :      jim-at-proscitech.com.au
Date: Thu, 27 Jan 2000 13:38:03 +1000
Subject: RE: SEM - Halophilic bacteria preparation

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I did some TEM work on the extreme holophile bacterias (they grow in saturated
NaCl solutions) some 30 years ago. Very difficult specimens, it seems no
fixation is complete and can prevent osmotic shock.

I have not tried SEM on halophiles, but I suggest this:
You could try excessive fixation, using 2 hours at room 20 degrees.
I would use a several molar solution of ammonium acetate to rinse the specimen
after fixation. Ammonium acetate is a volatile salt solution and leaves no
crystals after evaporation.
The still wet sample (mounted on a 10mm coverslip) could then be placed in a
glass Petrie dish which has a double layer of filter paper, saturated with
chloroform.
Place the closed Petrie dish in the fridge for a day or two. Warm the dish
before opening (to avoid condensation) and metal coat prior to SEM.

Ah, your first problem could be the fixation. The osmium (I'd forget GA), would
need to go into the bacteria's growth medium, or use vapour fixation only. Even
then, I expect much damage before any further processing.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Thursday, January 27, 2000 12:01 AM, Hyman, S.C.
[SMTP:sch10-at-leicester.ac.uk] wrote:
}
}
} Can anybody offer a method for the preparation of Halophilic bacteria for
} 'standard' SEM observation? post fixation washing appears to produce cell
} lysis!.



From daemon Wed Jan 26 23:12:32 2000



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Thu, 27 Jan 2000 00:31:22 -0800
Subject: No weather report please. Why not?

Contents Retrieved from Microscopy Listserver Archives
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Message-ID: {388FBBF2.61FF-at-mail.superlink.net}


Message-ID: {388FBBF2.61FF-at-mail.superlink.net}


Moreover, it is well known that weather may affect the quality of EM
samples. For instance, humidity is very critical for many EM techniques. I
utilized very unusual technique for holey-film preparation with
calcium-rhodanide. This technique is extremely sensitive for
humidity/temperature combination. When I was working in Russia (without
conditioner in the room), I was able predict the weather changes using that
technique. Again, it was tricky to work when temperature in the room was
around 7oC (at winter). The guys from East Coast may have something like
that. Why not to share experience how to work at different weather
conditions?

Have a good weather!

Sergey

} Date: Wed, 26 Jan 2000 17:52:26 -0800
} From: Paul Webster {pwebster-at-hei.org}
} Subject: Re:No weather report please
} To: MSA listserver submission {Microscopy-at-sparc5.Microscopy.Com}
} Reply-to: Paul Webster {pwebster-at-hei.org}
} X-Mailer: QuickMail Pro 1.5.4 (Mac)
} X-MIME-Autoconverted: from quoted-printable to 8bit by
ultra5.microscopy.com id
} TAA11085
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu
http://www.bol.ucla.edu/~sryazant




From daemon Thu Jan 27 06:51:48 2000



From: Ken Tiekotter :      tiekotte-at-up.edu
Date: Thu, 27 Jan 2000 00:57:54 -0800 (PST)
Subject: Re: sperm fixation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Hank and others,
I have had good success this Stefanini's buffered picric acid
paraformaldehyde (PAF) for spermatozoan. I do not have the fixative
formula at my fingertips, but the reference is: Nature Vol. 216, OCT 14.
1967.

I will look it up if you are interested and get back to you or you can
email me-at- tiekotte-at-up.edu.
-Ken

Ken Tiekotter
Dept. of Biology
The University of Portland
5000 N Willamette Blvd.
Portland, OR 97303



On Wed, 26 Jan 2000, hank adams wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Listservers,
} Can anyone lead me to a good reference for processing human sperm for TEM? Or if you have a procedure can you please forward the details.
} TIA
} Hank Adams
} Integrated Microscopy Core
} Molecular and Cell Biology
} Baylor College of Medicine
} Houston, TX
}
}



From daemon Thu Jan 27 06:51:58 2000



From: Shu-You Li :      syli-at-mail.uni-mainz.de
Date: Thu, 27 Jan 2000 11:36:12 +0100
Subject: Re: Cryostat info.

Contents Retrieved from Microscopy Listserver Archives
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Dear Dr. Erdos,

Perheps it is due to the difference between Netscape and IE. I got a blank
page with netscape but read it correctly with IE4.0.

Shu-You Li
**************************************************
Shu-You Li, Dr.
Institut fuer Physikalische Chemie
Johannes Guttenberg Universitaet
Jakob-Welder-Weg 11
D-55099 Mainz, Germany

E-mail: syli-at-mail.uni-mainz.de; syli16-at-hotmail.com
Fax: +49-6131-3923768
Tel: +49-6131-3923148(O)
**************************************************



} From: Greg Erdos {gwe-at-biotech.ufl.edu}
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Cryostat info.
} Date: Wed, 26 Jan 2000 09:19:58 -0500
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} For those who are unable to view the PDF file of Cryostat information that
} I posted, I have also posted it in ugly HTML. I am still trying to find
} out why some can read the PDF and others cannot. The Version of Acrobat
} does not seem to be the answer.
}
} My apologies to anyone who got frustrated.
} Once again the site is:
} www.biotech.ufl.edu/sems/
}
} Greg Erdos
} Gregory W. Erdos, Ph.D. Ph.
} 352-392-1295
} Assistant Director, Biotechnology Program
} PO Box 110580 Fax:
} 352-846-0251
} University of Florida
} Gainesville, FL 32611
}



From daemon Thu Jan 27 06:52:00 2000



From: Tony Bruton :      bruton-at-EMU.UNP.AC.ZA
Date: Thu, 27 Jan 2000 14:29:04 +0200
Subject: Re: No weather report please. Why not?

Contents Retrieved from Microscopy Listserver Archives
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Listers

I think that we are proceeding slightly tongue-in-cheek here - but to continue the 'serious' note;

The most weather sensitive task I have ever tackled is the production of formvar films. With the extremely high humidities we experience here there are times when the formvar will simply NOT separate from the substrate, despite 'air-conditioning'.

Tony



Tony Bruton
Head, Centre for Electron Microscopy
University of Natal, Pietermaritzburg
Tel +27 (0) 33 260 5155
Fax +27 (0) 33 260 5776
website via:http:www.nu.ac.za
Email:bruton-at-emu.unp.ac.za
postal address;
Private Bag X01,
Scottsville, 3209
KwaZulu-Natal
South Africa

} } } Sergey Ryazantsev {sryazant-at-ucla.edu} 01/27/00 10:31AM } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Moreover, it is well known that weather may affect the quality of EM
samples. For instance, humidity is very critical for many EM techniques. I
utilized very unusual technique for holey-film preparation with
calcium-rhodanide. This technique is extremely sensitive for
humidity/temperature combination. When I was working in Russia (without
conditioner in the room), I was able predict the weather changes using that
technique. Again, it was tricky to work when temperature in the room was
around 7oC (at winter). The guys from East Coast may have something like
that. Why not to share experience how to work at different weather
conditions?

Have a good weather!

Sergey

} Date: Wed, 26 Jan 2000 17:52:26 -0800
} From: Paul Webster {pwebster-at-hei.org}
} Subject: Re:No weather report please
} To: MSA listserver submission {Microscopy-at-sparc5.Microscopy.Com}
} Reply-to: Paul Webster {pwebster-at-hei.org}
} X-Mailer: QuickMail Pro 1.5.4 (Mac)
} X-MIME-Autoconverted: from quoted-printable to 8bit by
ultra5.microscopy.com id
} TAA11085
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu
http://www.bol.ucla.edu/~sryazant






From daemon Thu Jan 27 07:12:03 2000



From: Rick Powell at Nanoprobes :      rpowell-at-lihti.org
Date: Thu, 27 Jan 2000 08:02:45 -0500
Subject: Light microscopy:Fluorogold suppliers

Contents Retrieved from Microscopy Listserver Archives
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Hello Light Microscopists:

Can anyone tell me who currently makes and sells the retrograde neuronal
tracer, Fluorogold? We have a customer who is confusing it with our
FluoroNanogold products (not the first time this has happenned) and I would
lkke to point them to the right source!

Thanks,

Rick Powell


**********************************************************************
* NANOPROBES, Incorporated | US Toll-free: (877) 808-2101 *
* 25 East Loop Road, Suite 113 | Tel: (919) 510-0590 *
* Stony Brook, NY 11790-3350, | Fax: (919) 510-0590 *
* USA | rpowell-at-lihti.org *
* *
* NOW EASY TO FIND ON THE WEB: http://www.nanoprobes.com *
**********************************************************************




From daemon Thu Jan 27 07:36:36 2000



From: Robinson John :      emxray-at-server.uwindsor.ca
Date: Thu, 27 Jan 2000 08:09:45 -0500
Subject: Test

Contents Retrieved from Microscopy Listserver Archives
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From daemon Thu Jan 27 07:41:58 2000



From: RAHBARI, RAMIN :      RAMIN.RAHBARI-at-WL.com
Date: Thu, 27 Jan 2000 09:29:54 -0500
Subject: sperm fixation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Some time ago, Kent Christensen, Univ. of Michigan, (akc-at-umich.edu) kindly
supplied to me the following:


Zamboni's Fixative

FROM:rj.wilson-at-qut.edu.au (Russell J. Wilson)
The following is the composition of the Zamboni's Fixative

0.2M Na2HPO4.....................390mL
0.2M NaH2PO4.....................110mL
16% Paraformaldehyde.............25mL
Saturated Picric Acid............15mL
Distilled Water..................10mL
...........................................................................
.................

The main reference is: Stefanini et al., 1967, Nature 216:173.


Ramin Rahbari
PARKE-DAVIS Pharmaceutical Research
Worldwide Preclinical Safety
2800 Plymouth Road
Ann Arbor, MI 48105
Voice (734) 622-3383
Fax (734) 622-5001
Ramin.Rahbari-at-WL.COM


-----Original Message-----
} From: hank adams [mailto:hpadams-at-bcm.tmc.edu]
Sent: Wednesday, January 26, 2000 2:39 PM
To: 'microscopy-at-msa.microscopy.com'


Listservers,
Can anyone lead me to a good reference for processing human sperm
for TEM? Or if you have a procedure can you please forward the details.
TIA
Hank Adams
Integrated Microscopy Core
Molecular and Cell Biology
Baylor College of Medicine
Houston, TX



From daemon Thu Jan 27 17:57:49 2000



From: Sally Schroeter :      sally.schroeter-at-mcmail.vanderbilt.edu
Date: Thu, 27 Jan 2000 09:36:51 -0600
Subject: video to laptop display

Contents Retrieved from Microscopy Listserver Archives
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As far as I could discover, Dazzler and Snappy are for Windows
machines only. Are there similar devices available for Macintoshes?




From daemon Thu Jan 27 17:57:55 2000



From: john grazul :      grazul-at-physics.bell-labs.com
Date: Thu, 27 Jan 2000 11:29:14 -0500
Subject: Re: No weather report please. Why not?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




If anyone remembers I use to end all my e-mails to the listees with a quite
sarcastic weather and/or olfactory report from the garden state. Either no one
read my posts or they just didn't get the East Coast thing.

Cold, windy and something smells real odd under the Pulaski Skyway...Aloha!

John Grazul
Lucent

Sergey Ryazantsev wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Moreover, it is well known that weather may affect the quality of EM
} samples. For instance, humidity is very critical for many EM techniques. I
} utilized very unusual technique for holey-film preparation with
} calcium-rhodanide. This technique is extremely sensitive for
} humidity/temperature combination. When I was working in Russia (without
} conditioner in the room), I was able predict the weather changes using that
} technique. Again, it was tricky to work when temperature in the room was
} around 7oC (at winter). The guys from East Coast may have something like
} that. Why not to share experience how to work at different weather
} conditions?
}
} Have a good weather!
}
} Sergey
}
} } Date: Wed, 26 Jan 2000 17:52:26 -0800
} } From: Paul Webster {pwebster-at-hei.org}
} } Subject: Re:No weather report please
} } To: MSA listserver submission {Microscopy-at-sparc5.Microscopy.Com}
} } Reply-to: Paul Webster {pwebster-at-hei.org}
} } X-Mailer: QuickMail Pro 1.5.4 (Mac)
} } X-MIME-Autoconverted: from quoted-printable to 8bit by
} ultra5.microscopy.com id
} } TAA11085
} }
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } } Please keep weather reports private.
} } }
} } } Ann Fook
} }
} } Why? A little light heartedness never hurt anyone.
} } For the record, LA was sunny as usual today. Happy I don't live in CT
} anymore.
} }
} } Regards,
} }
} } Paul Webster, Ph.D.
} } Associate Scientist & Director
} } Ahmanson Advanced Electron Microscopy & Imaging Center
} } House Ear Institute
} } 2100 West Third St.
} } Los Angeles, CA 90057
} }
} } Phone: (213) 273-8026
} } Fax: (213) 413-6739
} } e-mail: pwebster-at-hei.org
} } http://www.hei.org/htm/aemi.htm
} }
} }
} }
} _____________________________________
}
} Sergey Ryazantsev Ph. D.
} Electron Microscopy
} UCLA School of Medicine
} Department of Biological Chemistry
} Box 951737
} Los Angeles, CA 90095-1737
}
} Phone: (310) 825-1144
} Pager: (310) 845-0248
} FAX (departmental): (310) 206-5272
} mailto:sryazant-at-ucla.edu
} http://www.bol.ucla.edu/~sryazant



From daemon Thu Jan 27 17:57:55 2000



From: J.F. Bailey :      jfb-at-uidaho.edu
Date: Thu, 27 Jan 2000 08:34:11 -0800
Subject: ultramicrotome service

Contents Retrieved from Microscopy Listserver Archives
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Is anyone aware of a company who would service an old LKB Ultratome
III? If so, please contact me at: jfb-at-uidaho.edu

Thank you.



From daemon Thu Jan 27 17:57:57 2000



From: Karen Zaruba :      kszaruba-at-MMM.COM
Date: Thu, 27 Jan 2000 10:50:50 -0600
Subject: Re: Light microscopy:Fluorogold suppliers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Molecular Probes sells hydroxystilbamidine, methanesulfonate (cat. # H-7599)
which I beleive is the active chemical in Fluorogold. (see paper by Martin W.
Wessendorf in Brain Res 553(1): 135-48. Jul 1991).

Karen Zaruba

P.S. I have no interest in Molecular Probes other than a satisfied customer.


Rick Powell at Nanoprobes wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hello Light Microscopists:
}
} Can anyone tell me who currently makes and sells the retrograde neuronal
} tracer, Fluorogold? We have a customer who is confusing it with our
} FluoroNanogold products (not the first time this has happenned) and I would
} lkke to point them to the right source!
}
} Thanks,
}
} Rick Powell
}
} **********************************************************************
} * NANOPROBES, Incorporated | US Toll-free: (877) 808-2101 *
} * 25 East Loop Road, Suite 113 | Tel: (919) 510-0590 *
} * Stony Brook, NY 11790-3350, | Fax: (919) 510-0590 *
} * USA | rpowell-at-lihti.org *
} * *
} * NOW EASY TO FIND ON THE WEB: http://www.nanoprobes.com *
} **********************************************************************

--
Karen S. Zaruba kszaruba-at-mmm.com
3M Company, St. Paul, MN




From daemon Thu Jan 27 17:57:57 2000



From: jmkrupp-at-cats.ucsc.edu (Jon Krupp)
Date: Thu, 27 Jan 2000 09:07:18 -0800
Subject: Cynthia Shannon-Are you there?

Contents Retrieved from Microscopy Listserver Archives
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Tried to respond to a message posted here from Cynthia Shannon re: a used
TEM, it keeps bouncing. Cynthia, if you are out there, how can I contact
you?

Jonathan Krupp
Microscopy & Imaging Lab
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu




From daemon Thu Jan 27 17:57:58 2000



From: hank adams :      hpadams-at-bcm.tmc.edu
Date: Thu, 27 Jan 2000 11:27:02 -0600
Subject: EM position opening

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


ELECTRON MICROSCOPY TECHNICIAN
The Integrated Microscopy Core, Department of Molecular and Cell Biology,
Baylor College of Medicine is expanding and has an immediate full-time
opening for an electron microscopy technician. The Integrated Microscopy
Core is a growing, state-of-the-art facility with 2 TEMs, deconvolution, laser
scanning confocal, 2 CCD-based fluorescent microscopes and multiple PC and
Silicon Graphics workstations for imaging software. The applicant should
have at least one year of experience in various aspects of sample
preparation for biological TEM including fixation, embedding, ultrathin
sectioning and staining. The applicant should have darkroom experience and
experience in the operation of TEMs. Other duties include preparation of
solutions, embedding media and the maintaining of records. The position
offers excellent opportunities for training in advanced light and electron
microscopy techniques, including immunofluorescence and immunogold labeling,
laser scanning confocal and deconvolution microscopy, as well as live
imaging of GFP-tagged proteins. Training in several image computer-based
imaging programs will also be available (PhotoShop, MetaMorph, SoftWorks).
The position requires a minimum of a Bachelors degree and will start as a
Lab Technician II; salary will be commensurate with experience, and includes
the standard Baylor benefits package.

Send CV and letter of research/technical interests to:

Hank Adams
Laboratory Manager
Integrated Microscopy Core
Department of Molecular and Cell Biology
Baylor College of Medicine
One Baylor Plaza
Houston, TX 77030
Email submissions to: hpadams-at-bcm.tmc.edu
Fax submissions to: 713 790 0545

Baylor College of Medicine is an Equal Opportunity, Affirmative Action and
Equal Access Employer.



From daemon Thu Jan 27 17:58:00 2000



From: Roger Moretz :      rcmoretz-at-excite.com
Date: Thu, 27 Jan 2000 09:45:24 -0800 (PST)
Subject: Re: Cryostat info.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I can't see how it can be netscape vs IE, since I got the blank page using
IE4. I haven't tried netscape or IE5 (have both at home--but not here at
work). Acrobat seems to work on every other PDF file I have opened (the
intranet and internet standard here for public documents in a read-only
setting), so I don't think the version of Acrobat is the problem either. I
tried Dr. Erdos' original work-around, but still got the blank page.
????


On Thu, 27 Jan 2000 11:36:12 +0100, Shu-You Li wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear Dr. Erdos,
}
} Perheps it is due to the difference between Netscape and IE. I got a
blank
} page with netscape but read it correctly with IE4.0.
}
} Shu-You Li
} **************************************************
} Shu-You Li, Dr.
} Institut fuer Physikalische Chemie
} Johannes Guttenberg Universitaet
} Jakob-Welder-Weg 11
} D-55099 Mainz, Germany
}
} E-mail: syli-at-mail.uni-mainz.de; syli16-at-hotmail.com
} Fax: +49-6131-3923768
} Tel: +49-6131-3923148(O)
} **************************************************
}
}
}
} } From: Greg Erdos {gwe-at-biotech.ufl.edu}
} } To: Microscopy-at-sparc5.microscopy.com
} } Subject: Cryostat info.
} } Date: Wed, 26 Jan 2000 09:19:58 -0500
} }
}
} ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
} } On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
}
} -----------------------------------------------------------------------.
} }
} }
} } For those who are unable to view the PDF file of Cryostat information
that
} } I posted, I have also posted it in ugly HTML. I am still trying to
find
} } out why some can read the PDF and others cannot. The Version of
Acrobat
} } does not seem to be the answer.
} }
} } My apologies to anyone who got frustrated.
} } Once again the site is:
} } www.biotech.ufl.edu/sems/
} }
} } Greg Erdos
} } Gregory W. Erdos, Ph.D. Ph.
} } 352-392-1295
} } Assistant Director, Biotechnology Program
} } PO Box 110580
Fax:
} } 352-846-0251
} } University of Florida
} } Gainesville, FL 32611
} }
}


Roger C Moretz, Ph.D.





_______________________________________________________
Get 100% FREE Internet Access powered by Excite
Visit http://freeworld.excite.com



From daemon Thu Jan 27 17:58:00 2000



From: Michael Bode :      mb-at-soft-imaging.com
Date: Thu, 27 Jan 2000 10:48:26 -0700
Subject: Re: USB or firewire cameras

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I think, there is some confusion out there about digital and analog
cameras and different protocols and interfaces. Woody's posting is
correct, but perhaps a look at some of the current implementations might
help:

1) Cameras come either with a digital output or analog output. As Woody
mentions, there are several different analog standards (PAL, NTSC,
RS-170,...) and formats for transmitting the data (RGB, S-VHS,
composite, ...)

2) Regardless of what the signal is, there must be some "device" that
translates the incoming signals into "numbers" that the computer can
understand. Sometimes this is implemented on the motherboards (USB), or
needs an additional card (frame grabber). It depends on the age of the
computer and its make and Operating system which of the different
options are supported.

3) Currently there are 3 ways of getting the signals into a PC (other
than serial RS-232 and parallel ports which are way to slow):

a) USB
b) 1394 or firewire
c) PCI boards

USB

USB is a serial interface that is now supported on most computers out of
the box. The bandwidth of a USB connection is a maximum of 12
MegaBIT/second, which translates of course into 1.5 Mega-BYTE per
second. This is too slow for video (about 4-5 MegaByte per second), but
enough for still images, unless the video is compressed. Compression is
OK for "consumer" video, but not acceptable for "scientific" video
unless it is lossless (JPEG and MPEG is usually not). Several devices on
the USB bus can compete for bandwidth.

1394

1394 (or firewire) is also a serial interface with a maximum bandwidth
of 400 Mega-BIT per second (or 50 Mega-BYTES per second). This is fast
enough for video. However, I believe that again more than one device can
be attached to a 1394 port which will again compete for bandwidth.
Firewire is not generally available on PCs (I believe the newer Macs
have it), so that for a firewire implementation on a PC normally a card
is necessary that fits into a PCI slot.

PCI

PCI boards, while a bit more cumbersome to install, have the highest
throughput. I think, they are now implementing a new PCI-X specification
that allows up to 1 GigaBYTE per second, i.e., about 20 times faster
than firewire. The reason is of course that PCI is a parallel standard
and not a serial like USB or firewire.

So, there are various ways to attach a camera:

Analog camera: There is no other way than to use a board to transform
the analog signals into digital signals and then send them to the
computer. This can be through a PCI or other card or other electronics
for example on the video card, but the translation is necessary.

Digital cameras: Digital cameras essentially put the digitizer into the
camera and then transmit digital signals. They can then be transferred
through USB (slow but available everywhere), firewire (faster, currently
on Macs (I believe) and PCs with additional card), or through boards for
the PCI bus (fastest, widely available, require board installation).

So, for most users (of PCs at least) there is currently no real
difference between using a firewire or other camera, as they either have
to install a PCI-} firewire card, or another PCI card for image
acquisition. That may change if the motherboard manufacturers start
building firewire circuitry into the motherboards and the operating
systems start supporting this option. For highest performance, however,
I believe that we will see PCI boards for some time to come. Firewire
may run into a performance problem in the future for image streams with
large images. A 1600x1200 image stream with 24 bit color and 30 frames
per second requires a bandwidth of about 170 MBytes/second uncompressed.

I hope I have not confused anybody with this.

Michael

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================


} ----------
} From: White, Woody N[SMTP:WOODY.N.WHITE-at-MCDERMOTT.COM]
} Sent: Wednesday, January 26, 2000 4:13:00 PM
} To: "Peter Guthrie" ; Microscopy-at-sparc5.Microscopy.Com
} Subject: Re:USB or firewire cameras
} Auto forwarded by a Rule
}
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Peter,

If you intend to use a "video" camera, you may need a capture card
rather
than,
or in addition to, a USB or Firewire interface. Most video cams are
analog
(NTSC in the US, SECAM or PAL elsewhere in the world). Others can
certainly
have digital circuits internal (like the new digital camcorders or the
PCcams
used for video conferencing on the net). ...You first need to
determine
the
source format.

Warren's suggestion implements an inexpensive external NTSC* video frame
grabber
(Snappy). Which will "grab" an analog video frame and digitize it.
*May do
PAL
too??

Parallel port I/O is a bit slow (or is that a byte slow :) for pictures
containing lots of data, but is cheap and works since the inherent
resolution of
typical NTSC video is { 640x480. USB is very much faster and Firewire
faster
yet (and usually a lot more $$ for the interface card). For
applications
other
than full frame rate/resolution streaming video, USB is fine.

Woody White


Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================




From daemon Thu Jan 27 17:58:03 2000



From: Damian :      dneuberger-at-mindspring.com
Date: Thu, 27 Jan 2000 12:50:15 -0600
Subject: Re: Cryostat info.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greg,

I just downloaded from Netscape 4.6 and opened it with Acrobat 3.0

Damian



From daemon Thu Jan 27 17:58:03 2000



From: Scott Wight :      scott.wight-at-nist.gov
Date: Thu, 27 Jan 2000 14:15:53 -0500
Subject: Re: USB or firewire cameras

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Michael

The IMAC DV comes with a firewire port standard and a firewire cable.
Connection to a Sony DV camcorder is easy and it gives you complete control
from the computer. See http://www.apple.com/firewire/ for more information.

To get firewire into a PCI (mac or windows) machine Sony sells this card
http://www.sel.sony.com/SEL/consumer/ss5/office/digitalvideo/minidvcamcorderspro
ducts/dvbk-2000_specs.shtml for around $350 which gives similar controls
for live video and digital stills.

No interest in either company except as a satisfied customer.
Scott


}
} 1394 (or firewire) is also a serial interface with a maximum bandwidth
} of 400 Mega-BIT per second (or 50 Mega-BYTES per second). This is fast
} enough for video. However, I believe that again more than one device can
} be attached to a 1394 port which will again compete for bandwidth.
} Firewire is not generally available on PCs (I believe the newer Macs
} have it), so that for a firewire implementation on a PC normally a card
} is necessary that fits into a PCI slot.
}
..snip...
} So, for most users (of PCs at least) there is currently no real
} difference between using a firewire or other camera, as they either have
} to install a PCI-} firewire card, or another PCI card for image
} acquisition. That may change if the motherboard manufacturers start
} building firewire circuitry into the motherboards and the operating
} systems start supporting this option. For highest performance, however,
} I believe that we will see PCI boards for some time to come. Firewire
} may run into a performance problem in the future for image streams with
} large images. A 1600x1200 image stream with 24 bit color and 30 frames
} per second requires a bandwidth of about 170 MBytes/second uncompressed.
}
} Michael Bode, Ph.D.

-------------------note: new mailing address----------------------
Scott Wight e-mail: scott.wight-at-nist.gov
NIST 222/A113 W voice: 301-975-3949
100 Bureau Dr STOP 8371 | fax: 301-417-1321
Gaithersburg, MD 20899-8371 \|/ disclaimer: Any opinion expressed
is my own and does not represent those of my employer.




From daemon Thu Jan 27 17:58:06 2000



From: Ekstrom, Harry :      harry.ekstrom-at-honeywell.com
Date: Thu, 27 Jan 2000 13:08:57 -0700
Subject: Re:No weather report please

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I agree...

Chill out Fook

Sunny and 70 in Phoenix

-----Original Message-----
} From: Paul Webster [mailto:pwebster-at-hei.org]
Sent: Wednesday, January 26, 2000 6:52 PM
To: MSA listserver submission


} Please keep weather reports private.
}
} Ann Fook

Why? A little light heartedness never hurt anyone.
For the record, LA was sunny as usual today. Happy I don't live in CT
anymore.

Regards,

Paul Webster, Ph.D.
Associate Scientist & Director
Ahmanson Advanced Electron Microscopy & Imaging Center
House Ear Institute
2100 West Third St.
Los Angeles, CA 90057

Phone: (213) 273-8026
Fax: (213) 413-6739
e-mail: pwebster-at-hei.org
http://www.hei.org/htm/aemi.htm



From daemon Thu Jan 27 17:57:45 2000



From: Pierre Ruterana :      ruterana-at-lermat8.ismra.fr
Date: Thu, 27 Jan 2000 16:47:52 -0800
Subject: Positions available in Europe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Collegues

We would apreciate to inform interested young researcher of the
following available positions in our research project.
Thank you very much for getting this circulated.
Pierre

Pierre Ruterana
Laboratoire d'Etude et de Recherche sur les Materiaux (LERMAT)
Unite associee CNRS No 6004
Institut Superieur de la Matiere et du Rayonnement(ISMRA)
6, Bd Marechal Juin
14050 Caen Cedex
France
Tel: (33 2) 31 45 26 53
Fax:(33 2) 31 45 26 60 e-mail: ruterana-at-lermat8.ismra.fr




Research Training Network EC Contract N¡: HPRN-CT-1999-00040
Interface analysis at atomic level and Properties of Advanced Materials
(IPAM)

Eight positions are immediately available, eligible young ( { 35 years)
researchers must be citizens
of EC or associated countries (Norway, Island, Israel, Lichtenstein,
Bulgaria, the Czech Republic,
Estonia, Hungary, Lithuania, Poland, Romania, Slovakia, Slovenia and
Letonia), however any
foreigner who has spent five years in an EC country may apply. Women
candidates are particularly
encouraged to apply and equal opportunity between women and men will
govern our choice.
Following the mobility criteria, the young researchers will not apply
for a position in their native
country.

1. POSTDOCTORAL POSITION at Fritz Haber Institute, Max Planck Society,
Berlin,
Germany
A postdoctoral position at the Fritz-Haber-Institut in Berlin (Germany)
is available in the group
"Surface morphology and growth of semiconductors" under the supervision
of Dr. Joerg
Neugebauer. The research will be mainly focused on the theoretical
modeling of electronic
properties and atomic structure of interfaces and interfacial defects
employing first principles total
energy calculations. The basic materials for this project will be
gallium nitride based
semiconducting layers where extended defects are well known to occur in
large concentrations. The
research will be performed in close collaboration with experimental,
industrial, and theoretical
partners within the EC. Strong interaction with the other groups is
therefore expected. The
successful candidate should have a PhD in Physics, Chemistry or
Materials Science, and have a
strong interest on microscopic simulations. Preference will be given to
candidates with strong
background in any (or several) of these fields: electronic structure
calculations, molecular
modeling, density functional theory, empirical potentials, and analysis
of transmission electron
microscopy measurements.
Interested candidates should send immediately a CV, list of
publications, name and address
(including email) of three references, preferably by email or fax, to:
Dr Joerg Neugebauer
E-mail: neugebauer-at-fhi-berlin.mpg.de, Phone: ++49 30 8413 4826, Fax:
++49 30
8413 4701, www: http://www.fhi-berlin.mpg.de/th/JG

2. POSTDOCTORAL POSITION at Universitat Politcnica de Catalunya,
Barcelona, Spain
A postdoctoral position is available at the department of Applied
Mathematics in the UPC. We are
seeking a computational materials scientist interested in modeling the
atomic structure of interfaces
and defects in crystals, mainly gallium nitride based materials. A PhD
in Physics, Materials Science
or related discipline and having experience with atomic simulations is
required. It is highly
desirable an ability to develop empirical interatomic potentials. It is
intended that he/she visits the
other laboratories working in the project to learn how to interpret the
experimental observations and
how to use the theoretical concepts.
Interested candidates should send immediately a CV, list of
publications, name and address
(including email) of three references, preferably by email or fax, to:
Prof. Anna Serra
E-Mail: a.serra-at-upc.es, Phone: ++34 93 401 68 86, Fax: ++ 34 93 401 18
25

3. A FULL TIME PhD STUDENT at CRHEA, Valbonne, France
These last years, CRHEA-CNRS has implemented an expertise in the growth
of
heteroepitaxial GaN layers on different substrates: sapphire, SiC and Si
by different techniques,
MBE, MOVPE and HVPE. The group has developed a proprietary Epitaxial
Lateral Overgrowth
(ELO) technology which allows to decrease by orders of magnitude the
density of dislocations in
GaN heteroepitaxial layers on sapphire, SiC or Si. Therefore, a great
interest in the procurement of
high quality GaN substrates currently exists. The successful candidate
will strongly support our
present effort to produce self-supported GaN of ELO quality by combining
ELO-MOVPE and
HVPE. Parallel to the growth, he will contribute to the development of
in depth analysis of basis
mechanisms linked to the generation and propagation of threading
dislocations(TDs). More
precisely, it is planned to determine the core structure of defects in
ELO GaN, their electronic
structures (by EELS), the mechanism of bending of these TDs, to
implement new ways of further
decreasing the density of dislocations. He will be able to use two
MOVPE, one HVPE reactor and
all basic characterisation tools (double X-ray diffraction,
magnetotransport, low temperature
photoluminescence, HRTEM,.).
Candidates should send immediately a CV, name and address (including
email) of two references,
preferably by email or fax, to:
Dr Pierre Gibart, email: Pierre.Gibart-at-crhea.cnrs.fr, Tel: ++33 4 93 95
42 27, Fax: ++33 4
93 95 83 61
CHREA-CNRS is located at the French Riviera near Nice ( see:
http://www.crhea.cnrs.fr/)

4. POSTDOCTORAL POSITION at ISMRA Caen, France
Candidates should preferably have a Phd with experience in electron
microscopy and/or atomic
structure modeling. The project will involve experimental high
resolution electron microscopy and
image analysis. In parallel, atomic structure modeling of defects and
interfaces will use empirical
and tight binding methods. A connection will be established with ab
initio techniques developed in
partner groups and the successful candidate will undertake quantitative
comparison of experimental
and simulated images. The overall aim is the understanding of the role
of defects and interfaces on
the optoelectronic properties in the Ga based nitride semiconductors.
Candidates should send immediately a CV, list of publications, name and
address (including email)
of three references, preferably by email or fax, to:
Dr Gerard Nouet on ++33 2 31 45 26 47,
email:gerard.nouet-at-labolermat.ismra.fr or Dr
Pierre Ruterana on ++33 2 31 45 26 53 email: ruterana-at-lermat8.ismra.fr

5. POSTDOCTORAL POSITION at University of Liverpool, Great Britain
A three year full-time appointment funded by the European Commission is
available to study defect
mechanisms in gallium nitride based electronic device structures within
the III-V semiconductor
materials group. Candidates should preferably have postgraduate
experience in the growth or
processing of semiconductor device materials. The project will involve
the chemical beam epitaxy
of GaN based materials and the fabrication of model device structures.
The influence of processing
parameters on defect propagation will be investigated using analytical
methods such as electron
microscopy, Raman spectroscopy and surface analytical techniques. This
appointment is part of a
Research Training Network and eligible candidates must be citizens of EC
member countries other
than the United Kingdom.
Candidates should send immediately a CV, list of publications, name and
address (including email)
of three references, preferably by email or fax, to:
Dr Paul Chalker: ++44 151 794 4313, email pchalker-at-liv.ac.uk or Prof
Robert Pond: ++44
151 794 43 13 / 46 60, email R.C.Pond-at-liverpool.ac.uk

6. POSTDOCTORAL POSITION at UniversitŠt Erlangen-NŸrnberg, Germany
Focus of the work in Erlangen university will be on direct correlation
of structural, optical and
electrical properties of extended defects in (i) group-III nitrides (ii)
group III-nitride based
heterostructures. Experimental work is based on (scanning) transmission
electron microscopy
((S)TEM) at all levels of resolution. These comprise high resolution
imaging with atomic
resolution, optical characterisation by cathodoluminescence in the STEM
and analysis of electrical
properties (electrical activity of extended defects, diffusion length of
minority carriers) by the
electron beam induced current (EBIC) both in the SEM and the STEM.
Theoretical analyses are
based on TEM contrast simulation for defect analysis, analysis of the
tetragonal distortion from
high resolution TEM images and finite element simulations of the
strained state of heterostructures.
Candidates should send immediately a CV, list of publications, name and
address (including email)
of three references, preferably by email or fax, to:
Prof Horst Strunk, Tel: ++49 9131 85 2 8601, Fax: ++49 9131 85 2 8602,
email:
strunk-at-cmp03ww7.ww.uni-erlangen.de, Dr Martin Albrecht, Tel.: ++49
9131 85 2 8613,
Fax: ++49 9131 85 2 8602, e-mail: albrecht-at-cmp04ww7.ww.uni-erlangen.de,


7. POSTDOCTORAL POSITION at the Aristotle University of Thessaloniki,
Greece
A research opportunity is available for postdoctoral candidates with a
background in Electron
Microscopy, Crystal Growth, Materials Science. The primary
responsibility of the candidate will
be the study of the structure and properties of the heterophase
interfaces between thin films on
gallium nitride based materials. The project will offer the necessary
training for the specific skills
to meet the requirements of the job.
Candidates should send immediately a CV, list of publications, and name
and address (including
email) of three references, preferably by email or fax, to:
Prof Philomela Komninou, Tel: ++30 31 99 81 95 / Fax: ++30 31 99 80 61
e-mail: komnhnoy-at-auth.gr

8. A FULL TIME PhD STUDENT at The University of Cambridge, Great Britain
A three year research studentship position leading to a PhD degree is
available to study the
microscopy and analysis of defects in gallium nitride layers and device
structures. This exciting
project will use a wide range of state-of-the-art electron microscopy
and analysis techniques to
study the atomic structure of defects (using high resolution electron
microscopy), their chemical
composition and electronic properties (using x-ray spectroscopy and
electron energy loss
spectroscopy), including which defects give rise to states in the band
gap. This project is part of a
European Research Training Network aimed at optimising devices in
GaN-based materials. The
research student will form strong links with the other European partners
in this project. Eligible
candidates should have a top quality degree in physics, chemistry,
materials science or electrical
engineering.
Candidates should send immediately a CV, name and address (including
email) of two references,
preferably by email or fax, to:
Prof Colin Humphreys on +44 1223 334457, email
{colin.humphreys-at-msm.cam.ac.uk} , or
Dr Dave Tricker on +44 1223 334469, email {dmt1000-at-cus.cam.ac.uk}


The Candidates will gain time by sending a copy of their CV also to
Prof. Philomela
Komninou, leader of the Training Programme. Tel: ++30 31 99 81 95 /
Fax: ++30 31 99 80 61
e-mail: komnhnoy-at-auth.gr; Please indicate the position of interest.


Caen, January 27 2000
Dr. Pierre Ruterana
Coordinator



From daemon Fri Jan 28 07:53:17 2000



From: jim :      jim-at-proscitech.com.au
Date: Fri, 28 Jan 2000 15:13:15 +1000
Subject: RE: Shelf life of LR-Gold

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


It seems nobody replied to Michaels question. Perhaps because there is no
single answer. LR White and LR Gold I would expect to have a very similar
shelf-life - under similar conditions.

The trouble is to know the starting point. LR White slowly "goes off" from
when the catalyst is added. At room temperature or higher this happens at a
much faster rate than when it's kept refrigerated. Catalyzed LR White could be
kept at the room temperatures for some months. Because the shipping time (even
by air) to our home-market (Australia) from the UK is too long and often at
high temperatures, much and an indeterminable part of the shelf-life would be
expired prior to sale.

Consequently we only procure uncatalysed LR White and the end-user must add and
thoroughly mix the catalyst prior to first use. Our users expect a full year of
refrigerated shelf-life. Shelf-life is really a "when, how long and at what
temperature" question.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Tuesday, January 25, 2000 4:52 PM, Michael Reiner
[SMTP:michael.reiner-at-Smail.Uni-Koeln.de] wrote:
}
}
} Dear members of the list,
}
} first, I would like to wish you all the best for the new year.
} Now my question:
} Does anyone know the shelf-life/usability of LR-Gold stored in a fridge?
}
} Is it that delicate as LR-White (Meanwhile I don?t use LR-W older than
} half a year). My bottle which was not opened many times, could be
} roundabout three years old.
}
} Thanks a lot,
} Michael
}
} Michael Reiner
} Department of Anatomy I
} University of Cologne
} Germany
} michael.reiner-at-smail.uni-koeln.de



From daemon Fri Jan 28 07:53:25 2000



From: Witold Zielinski :      WIZIEL-at-inmat.pw.edu.pl
Date: Fri, 28 Jan 2000 09:42:49 CET
Subject: Re:No weather report please

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


*Date sent: 26 Jan 00 17:52:26 -0800
*From: Paul Webster {pwebster-at-hei.org}
*Subject: Re:No weather report please
*To: MSA listserver submission {Microscopy-at-sparc5.microscopy.com}
*Send reply to: Paul Webster {pwebster-at-hei.org}

*------------------------------------------------------------------------
*The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Fri Jan 28 07:53:33 2000



From: Timothy Dimitri :      tdimitri-at-us.ibm.com
Date: Fri, 28 Jan 2000 07:56:57 -0500
Subject: SEM - Manufactures of Microcleave tools for silicon wafers???

Contents Retrieved from Microscopy Listserver Archives
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Hello,

I am looking for the names of any manufactures (other than SELA) that
have a product that can cleave sub micron features on silicon wafers...

Thank you

Timothy Dimitri
ASTC Failure Analysis Laboratory
IBM Microelectronics Division



From daemon Fri Jan 28 08:08:17 2000



From: McGill, Ricky L :      rlmcgill-at-eastman.com (by way of Nestor J.
Date: Fri, 28 Jan 2000 07:55:51 -0600
Subject: Spring 2000 AReMS Meeting

Contents Retrieved from Microscopy Listserver Archives
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Dear list members,
The intent of this note is to formally announce a call for papers
for the upcoming Spring 2000 AReMS (Appalachian Regional Microscopy
Society) meeting to be held in Raleigh, NC.The meeting dates are March 30
and 31.
The theme for this meeting is "Recent Advances in Microscopy for
2000",specifically we would like to focus on recent (but not limited to)
advances in microscopical instrumentation and applications therefrom.


Please forward any abstracts, papers or related items to me at
mailto:\\rlmcgill-at-eastman.com or you may contact me at the number
below.

The most recent meeting info is -at-
http://www.wise.virginia.edu/cvc/arems/raleigh.html

Thanks in advance for your interest!


Rick McGill
Microscopy Research
Eastman Chemical Company
- - - Phone: (423) 229-5473
- - - Fax: (423) 229-4558
- - - e-mail: rlmcgill-at-eastman.com




From daemon Sat Jan 29 08:54:12 2000



From: Keith Ryan :      KPR-at-wpo.nerc.ac.uk
Date: Fri, 28 Jan 2000 14:13:01 +0000
Subject: Re: RE: No weather report please

Contents Retrieved from Microscopy Listserver Archives
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You should logon to Histonet - they're much friendlier!

Had a beautiful drive from old Plymouth to Reading, Berkshire, with
my retired boss yesterday to collect x-ray microanalysis equipment.
Clear blue sky, -2 to +5 degrees. Saw the most gorgeous scenes of
trees covered by thick hoar frost - photographers' dream. Met two
nice ladies - Hello, Jill and Hilary! Thanks for the help!

Keith Ryan
Marine Biological Association of the UK
PS - Don't read this if you don't like the weather !
PPS - See, Paul, I did get there!
PPS - Hello, Daniele - time to write!



From daemon Sat Jan 29 08:54:09 2000



From: Keith Ryan :      KPR-at-wpo.nerc.ac.uk
Date: Fri, 28 Jan 2000 14:23:12 +0000
Subject: Re: No weather report please. Why not?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


You ought to logon to "Histonet" - they're much friendlier!

I agree about the weather being important to EM. 20-30 years ago we
had some seaweed hanging in the microtome room (about 100 m from the
sea). If it was damp we didn't even try cutting some resins!

Had a car trip yesterday from old Plymouth to Reading, Berkshire,
collect EM equipment., -2 to +5 degrees, clear blue sky, gorgeous
scenes of thick hoar frost on the winter trees - a photographers'
dream. Bonus - met two nice girls - Hello, Jill and Hilary, thanks for
the help!

Keith Ryan
Marine Biological Association of the UK
PS - Don't read this if you don't like weather!
PPS - Paul, I made it!
PPPS - Hello, Daniele, its time you wrote!



From daemon Sat Jan 29 08:54:18 2000



From: Gib Ahlstrand :      giba-at-puccini.cdl.umn.edu
Date: Fri, 28 Jan 2000 11:14:34 -0600 (CST)
Subject: weather & Formvar films

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Dear Microlisters,

Neither rain nor sleet, snow nor heat, humidity, hell nor highwater can keep me
from getting Formvar films off glass slides. Only time can...see below.

My secret? Just rinse glass slides - both sides & all around edges, except for
the end you are holding onto - with 95% ethanol and air dry. Then use right away
- dunk into Formvar solution (.25 to .5% w/v in ethylene dichloride), drain and
air dry. Score around edges to break film and float off onto clean water
surface. I like to score the edge by using the corner of a razor blade to score
on the top surface of the slide, near the edge, in addition to scoring the
actual corner edge of the slide with the blade held perpendicular to the edge -
know what I mean? Also, score across the slide near the "top" edge of the
Formvar film, near the end you are holding on to, for clean release of the end
of the film.

Second point, Formvar film solutions older that 3 months tend to stick to glass.
I've been tracking that for years. Put mix date on bottle of fresh solution,
after 3 months expect poor release effects to appear.

Gib

P.S. I tend to agree with Fook that this forum should not be used to discuss the
weather ONLY, but if someome wants to put a current local weather "tag" at the
end, AFTER the Microscopy stuff, with their signature, thats OK with me.
Everyone is entitled to their (short) bit of poetry, sage sayings, or weather
commentary there.

For example: "The weather here is unremarkable at this time."

Gib Ahlstrand
Electron Optical Facility, University of Minnesota, Dept. Plant Pathology
495 Borlaug Hall, St. Paul, MN. USA. 55108 (612)625-8249
612-625-9728 FAX, giba-at-puccini.cdl.umn.edu
http://biosci.umn.edu/MIC/consortium.html



From daemon Sat Jan 29 08:54:19 2000



From: Ray D. Twesten :      twesten-at-uiuc.edu
Date: Fri, 28 Jan 2000 11:52:18 -0600
Subject: TEM: Help with Microtoming Nanophase Metals

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Dear All,
I have a user who is trying to make TEM samples from nanophase metals.
They come out of the process as a fine (10 to 100um) powder. The study is
to compare the microstructure at different processing temperatures (77K to
400K). The current sample preparation technique is to embed the powder in
epoxy, slice and polish, and finish with l-N2 ion milling (BTW, direct
dispersion of the powder does not work as the edges are too thick). There
are several problems with this technique, the worst being that we have
found these materials age rapidly even at room temperature. The epoxy cure
and ion milling thermal budgets may be a problem.
I am considering ultramicrotomy for these samples, but I have zero
experience in this field. McMahon and Malis (1995 Micro Res & Tech v31 267)
worked on a similar system and outline the use of thermally cured LR-White
as the embedding material, so I think it is an appropriate option, but I am
worried about the thermal budget. My question is:
Does any one have experience with low-temperature, UV cured resins for
materials of these type? If so I would greatly appreciate any advice.

Thanks in advance,
Ray


*************************
Ray D. Twesten, PhD
Center for Microanalysis of Materials
University of Illinois
(217) 244-6177 fax:(217) 244-2278




From daemon Sat Jan 29 08:54:22 2000



From: Tobias Baskin :      BaskinT-at-missouri.edu
Date: Fri, 28 Jan 2000 15:21:14 -0600
Subject: Re: ICC label on cell walls

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The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Please see request below. If you have any suggestions, either send to the list and I will forward them to the investigator, or send them directly to Dale.
Thanks for the input.
Debby Sherman

--------------------------------------


Greetings,
I can make two suggestions.

1) Ignore the staining in the cell wall. Since you know that
your protein of interest is not there and you know also that
secondary cell wall must be outside of the cell, this should not
interfere with staning inside the cell.

2) Try to pre-absorb with "wood". The idea here would be to
incubate your serum with some sort of wood pulp, and then spin out
the wood pulp presumably taking with it all the ab's that bind wood,
but leaving behind the ones of interst. I am not sure how best to
make a suitable pulp of wood. Maybe take a pencil sharpener and grind
a dowell, and then grind the shavings further in a mortar and pestle
or maybe use a homogenizer of some sort. I am guessing wildly here.
Certainly the wood bits should be easy to spin after absorption.

Hope this helps,
Tobias.


}
} Date: Thursday, January 27, 2000
} } From: Dale Karlson {dtk-at-omni.cc.purdue.edu}
}
}
}
} QUESTION: How to minimize artifact labelling with secondary cell walls
}
}
} To whom it may concern:
}
} I am attempting to localize protein in a woody plant and consistently
} observe an interaction with secondary cell walls. This is an artifact, we
} know that the protein does not exist in the cell wall. We are using a
} polyclonal antibody that was raised against a protein that was excised
} from an SDS gel, suspended in Freund's complete adjuvant and used for the
} immunization (in chicken). Chicken antibodies were purified by an
} ammonium sulfate precipiation method described by Song et al. (Song, C.S.,
} J.H. Yu, D.H. Bai, P.Y. Hester, and K.H. Kim. 1985. Antibodies to the
} 5-subunit of insulin receptor from eggs of immunized hens. Journal of
} Immunology 135: 3354-3359). I have tried Western blot affinity
} purification of the antigen and antibody and it has not solved this
} problem. We do not have access to a "purified" form of the protein, so
} affinity purification with a purified protein is not an option.
}
} It is obvious that the chicken had an "allergy" that was not visible
} during our screening process (with western blots) to select the host
} animal. The chicken obviously has specific antibodies to some secondary
} wall component, and I would like to know what it might be and how I could
} remove this artifact.
}
} Any input would be greatly appreciated.
}
}
} -------------------------------------------
}
} Debbie,
}
} let me know if this is suitable..or if it is way too long etc.
}
} Thanks,
}
} -Dale
}
}
}
}
}
} _______________________________________________________________________
}
} Dale Karlson .***. .***. .***.
} 1165 Horticulture Building * | | | * | | | * * | | | *
} Purdue University * | | | * * | | | * * | | | *
} W.Lafayette. IN 47907-1165 * | | | * * | | | * | | | *
} '***' '***' '***'
}
} Home Phone: (765) 742-8379
} Lab Phone: (765) 494-1345
} _______________________________________________________________________

_ ____ ^ __ ____ Tobias I. Baskin
/ \ / / \ / \ \ University of Missouri
/ | / / \ \ \ Biological Sciences
/___/ /__ /___ \ \ \__ 109 Tucker Hall
/ / / \ \ \ Columbia, MO 65211-7400 USA
/ / / \ \ \ voice: 573-882-0173
/ /____ / \ \__/ \____ fax: 573-882-0123



From daemon Sat Jan 29 08:54:27 2000



From: Jim Goodman :      jgoodman-at-utsi.edu
Date: Fri, 28 Jan 2000 14:07:42 -0800
Subject: TEM

Contents Retrieved from Microscopy Listserver Archives
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To All,

The decision has been made to get rid of the following piece of equipment. We obtained the unit approximately five years ago when an outside Contractor brought the system very near operational state. Numerous distractions and circumstances have prevented the TEM from becoming the valuable research investigative tool we had planned.

HITACHI H-600 TEM

1) Model H-600-1 Analytical Electron Microscope
2) Model H-6015 EDX Interface Kit
3) Model H-6012 Micro-Diffraction Unit
4) Spot Scan
5) Model H-6006 Auto Data Display Unit
6) Reduced Area Scan Unit
7) Polaroid Camera w/ Adaptor
8) Model H-6007 High Resolution CRT
9) Model H-5001-C Cobling Holder
10) 2 ea. Overhauled Mechanical Vacuum Pumps & 1 ea. Diffusion Pump

Anyone interested in requesting a Bid Form should contact me at following address:

Jim Goodman
University of Tennessee Space Institute (UTSI)
411 B. H. Goethert Pkwy.
Tullahoma, TN 37388
TEL: (931) 393-7494
FAX: (931) 393-7543
e-mail: jgoodman-at-utsi.edu



From daemon Sat Jan 29 08:54:21 2000



From: Jo Dee :      jofish-at-burnham-inst.org
Date: Fri, 28 Jan 2000 22:32:52 -0800
Subject: Re: No weather report please

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Yes, I also agree. Today in San Diego it is about 68 degrees with 76% humidity.
Great for cryoultramicrotomy and regular cutting!
Take care all,
Jo Dee

Witold Zielinski wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} *Date sent: 26 Jan 00 17:52:26 -0800
} *From: Paul Webster {pwebster-at-hei.org}
} *Subject: Re:No weather report please
} *To: MSA listserver submission {Microscopy-at-sparc5.microscopy.com}
} *Send reply to: Paul Webster {pwebster-at-hei.org}
}
} *------------------------------------------------------------------------
} *The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} *To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} *On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} *-----------------------------------------------------------------------.
} *
} *
} *} Please keep weather reports private.
} *}
} *} Ann Fook
} *
} *Why? A little light heartedness never hurt anyone.
} *For the record, LA was sunny as usual today. Happy I don't live in CT anymore.
} *
} *Regards,
} *
} *Paul Webster, Ph.D.
} *Associate Scientist & Director
} *Ahmanson Advanced Electron Microscopy & Imaging Center
} *House Ear Institute
} *2100 West Third St.
} *Los Angeles, CA 90057
} *
} *Phone: (213) 273-8026
} *Fax: (213) 413-6739
} *e-mail: pwebster-at-hei.org
} *http://www.hei.org/htm/aemi.htm
} *
} *
} I agree with you Paul.
} In Warsaw, Poland yesterday was snow on the ground today is
} rain and no chance for sunshine.
} Stay cool,
} Witold

--
Jo Dee Fish
Electron Microscopy Assistant
The Burnham Institute
10901 N. Torrey Pines Rd.
La Jolla, CA 92037
858-646-3100 ext.3620




From daemon Sat Jan 29 08:54:52 2000



From: Keith Ryan :      KPR-at-wpo.nerc.ac.uk
Date: Sat, 29 Jan 2000 11:13:44 +0000
Subject: Double weather report - sorry

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The first one took a long time to appear - so I thought it hadn't made
it. Must've encountered head winds!

Keith



From daemon Sat Jan 29 08:54:53 2000



From: Keith Ryan :      KPR-at-wpo.nerc.ac.uk
Date: Sat, 29 Jan 2000 11:17:31 +0000
Subject: Re: No weather report please. Why not?

Contents Retrieved from Microscopy Listserver Archives
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Sorry for wasting time with two messages.

The first weather report took so long to appear that I thought it
hadn't made it. Must've encountered headwinds!

Enough - Keith Ryan
Marine Biological Association of the UK
- where its now wet and windy
PS - Hi Daniele



From daemon Sat Jan 29 13:04:49 2000



From: Diane G. Miller :      millerd-at-coho.net
Date: Sat, 29 Jan 2000 09:12:49 -0600
Subject: Cutting live cells on Vibratome

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Is anyone cutting live cells on a vibratome? These are from cell cultures.
If anyone has had experience with this, I would appreciate the information.

Thanks
Diane
millerd-at-coho.net




From daemon Sat Jan 29 13:04:48 2000



From: Cochran :      fisher-at-meganet.net
Date: Sat, 29 Jan 2000 10:30:45 -0500
Subject: service providers needed

Contents Retrieved from Microscopy Listserver Archives
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Hi All,

This request is posted on behalf of an associate who does not subscribe
to this list. He has assumed responsibility for the following equipment
and is looking for service providers. Equipment is located in south
central Massachusetts.

Jeol 840A sem
Kevex delta 5 EDS & XRF with Syquest drive upgrade

He should be contacted off line by e-mail at LapradeB-at-burle-eo.com

Thanx,
Ray



From daemon Sat Jan 29 13:04:52 2000



From: IMZartTchr-at-aol.com
Date: Sat, 29 Jan 2000 13:09:29 EST
Subject: microbiological staining technique

Contents Retrieved from Microscopy Listserver Archives
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what microbiological staining technique could be used to allow you
to differeniate Bordetella bronchiseptica from Vibrio cholerae with certainty?



From daemon Sun Jan 30 08:44:58 2000



From: Damian :      dneuberger-at-mindspring.com
Date: Sat, 29 Jan 2000 18:40:43 -0600
Subject: Re: USB or firewire cameras

Contents Retrieved from Microscopy Listserver Archives
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Michael,

FYI, I just tested an Optronics digital camera that had a on board firewire
connection to a Gateway 366MHz notebook computer. One can also get PCMCIA
card to connect to most notebook computers. Nice camera but should be for
$13k, without notebook computer!

Damian


} 1394 (or firewire) is also a serial interface with a maximum bandwidth
} of 400 Mega-BIT per second (or 50 Mega-BYTES per second). This is fast
} enough for video. However, I believe that again more than one device can
} be attached to a 1394 port which will again compete for bandwidth.
} Firewire is not generally available on PCs (I believe the newer Macs
} have it), so that for a firewire implementation on a PC normally a card
} is necessary that fits into a PCI slot.



From daemon Sun Jan 30 08:45:03 2000



From: Dr. Raj Lartius :      rlartius-at-novascan.com
Date: Sat, 29 Jan 2000 21:54:07 -0600
Subject: Looking for Ion Beam sputter coater or similar

Contents Retrieved from Microscopy Listserver Archives
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I'm trying to find a used ion beam sputter coater or something similar...
The system I am used to is the old VCR group ion beam sputter coater. Any
leads that you might have would be greatly appreciated...

Thanks,

Raj

*********************************************
Raj Lartius, Ph.D.
NovaScan Technologies
Iowa State University Research Park
2501 North Loop Drive
Ames, Iowa USA 50010

Email: rlartius-at-novascan.com
Web: www.novascan.com
**********************************************



From daemon Sun Jan 30 12:05:00 2000



From: bobrob-at-uswest.net
Date: Sun, 30 Jan 2000 10:18:55 -0700
Subject: TEM/EDS Detector Wanted

Contents Retrieved from Microscopy Listserver Archives
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We are interested in acquiring an EDS detector for our JEOL
JEM-1200EXII.
Will trade a Kevex Be-windowed 30mm2 that was formerly attached to an
EM400 for a Kevex/TN/Noran 10mm2/30mm2 Be or UTW. Need detector
only (no MCA, P. Processor, etc). Will purchase or trade.

If you have a suitable detector and are in a position to trade/sell
immediately,
please reply off line to sender.

Bob Roberts
EM Lab Services, Inc
2409 S. Rural Rd
Tempe, Arizona 85282
(480) 967-3946



From daemon Mon Jan 31 07:34:26 2000



From: David Knecht :      knecht-at-uconnvm.uconn.edu
Date: Sun, 30 Jan 2000 11:53:22 -0500
Subject: Double immunofluorescence artifacts

Contents Retrieved from Microscopy Listserver Archives
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I have been reading about FRET and have a question about dual label
imaging with probes like FITC/RHo or Cy3/Cy5. We have to worry about
excitation of the long wavelength probe at the shorter probe
wavelength, and FRET as two ways in which we can be mislead about the
co-localization of two probes. I am wondering to what extent one
also has to worry about non-FRET energy transfer. It seems that
there is some possibility that, for example, Cy5 could become excited
by absorbing photons from Cy3 emission. My presumption is that the
density of photons is low, and this would limit the effect, but it
seems that as proximity gets closer, the chances of this radiative
exchange (rather than resonance exchange) would become greater. Are
there any experimental guidelines as to when to worry about this?
Thanks- Dave
--

************************************************************

Dr. David Knecht
Department of Molecular and Cell Biology
University of Connecticut
75 N. Eagleville Rd. U-125
Storrs, CT 06269
Knecht-at-uconnvm.uconn.edu
860-486-2200 860-486-4331 (fax)
home page: http://www.sp.uconn.edu/~mcbstaff/knecht/knecht.html
************************************************************



From daemon Mon Jan 31 07:34:26 2000



From: Roger Moretz :      rcmoretz-at-excite.com
Date: Sun, 30 Jan 2000 20:08:22 -0800 (PST)
Subject: Re: Bio Cryo

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On Mon, 24 Jan 2000 13:33:28 -0500, Greg Erdos wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Periodically questions appear on this list concerning the use of
cryostats
} for cutting histological sections. I usually reply to the sender off
line
} and offer a copy of a handout that I got from a workshop at a Histochem
} Meeting some years ago. Even though it is old, cryostat sectioning has
not
} changed a lot. I think there is some valuable info there, especially for

} beginners. I thought it might be helpful to make this available on the
net
} for whomsoever might want to take a look.
} It can be found at :
} http://www.biotech.ufl.edu/sems/
}
} Look for the snowflake
}
} It was written by Bruce Quinn, then of MIT. I hope he has no
objections
} to my posting it.
}
} Greg Erdos
} Gregory W. Erdos, Ph.D. Ph.
352-392-1295
} Assistant Director, Biotechnology Program
} PO Box 110580
Fax:
} 352-846-0251
} University of Florida
} Gainesville, FL 32611
}
}
}
Finally got onto things here at home, and using Netscape 4.5 and Acrobat
3.0, the document opens up the way it should. Thanks for the info and link,
Greg.

Roger C Moretz, Ph.D.





_______________________________________________________
Get 100% FREE Internet Access powered by Excite
Visit http://freeworld.excite.com



From daemon Mon Jan 31 07:34:37 2000



From: simon watkins :      swatkins+-at-pitt.edu
Date: Mon, 31 Jan 2000 08:57:00 -0500
Subject: 2nd annual course in fluorescence microscopy

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Dear Timothy

A method I've used for controlling the position of the cleave in silicon
wafers is to use focused ion beams (FIBs) to mill micro-cleaving grooves
into the silicon. I found that the grooves can determine the position of a
cleave to within 200 nm.

If you want any more details I can forward you a pre-print on the technique.

Richard

--------------------------------------------------------------
Richard M Langford

Department of Materials, University of Oxford
Parks Road, Oxford, OX1 3PH, UK

Tel: +44 (0)1865 273729, Fax: +44 (0)1865 273794
email: richard.langford-at-materials.oxford.ac.uk

----- Original Message -----
} From: Timothy Dimitri {tdimitri-at-us.ibm.com}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Friday, January 28, 2000 12:56 PM


Folks:
I thought I should let you all know about the Second annual course in
Quantitative Fluorescence Microscopy to be taught between june 19 and 24th
2000 at the Mount Desert Island Marine Biology Laboratories in Arcadia
National Park in Maine. This team taught course led by Dave Piston
(Vanderbilt), Mike Nathanson (Yale), Guillermo Romero (Pittsburgh) and
myself focusses specifically on the development and application of modern
fluorescence microscopic methods. This intensive course covers all aspects
of the technology from microscope and dye design, cameras, confocal
microscopy, live cell microscopy, multiphoton microscopy and GFP.
Considerable attention is also given to quantitative analysis in 2 and 3
dimensions and time. The specific focus of the course allows an in depth
treatment of these methods. The goal of the course it to teach students how
to best implement these methods within their labs, using either their own
cells and tissues or using material supplied by the course. An extensive
array instrumentation, provided by all the major microscope and associated
software, hardware and camera manufacturers will be available for students
to use.
Last year it was a very successful event and we were encouraged to give the
course again. A full description of the course lectures together with
lecture outlines, registration etc. is available at
http://sbic6.sbic.pitt.edu/microscopy. I would encourage you to spread the
word, or sign up if you are interested. The total number of students is
limited to 20, enrollment is decided by the course faculty.
If you have any further questions please feel free to contact me
Thanks
Simon


-----------------------------------------
Simon C. Watkins Ph.D. MRCPath
Associate Professor
Director: Center for Biologic Imaging
University of Pittsburgh
Pittsburgh PA 15261
tel:412-648-3051
fax:412-648-8330
URL: http://sbic6.sbic.pitt.edu
-----------------------------------------
Simon C. Watkins Ph.D. MRCPath
Associate Professor
Director: Center for Biologic Imaging
University of Pittsburgh
Pittsburgh PA 15261
tel:412-648-3051
fax:412-648-8330
URL: http://sbic6.sbic.pitt.edu



From daemon Mon Jan 31 18:30:41 2000



From: Leona Cohen-Gould :      lcgould-at-mail.med.cornell.edu
Date: Mon, 31 Jan 2000 11:02:16 -0400
Subject: Re: microbiological staining technique

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At 1:09 PM -0500 1/29/0, "IMZartTchr-at-aol.com"-at-sparc5.Microscopy.Com wrote:
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***********
If you don't get an answer from MSA people, try the listserver for the
histologists out there:
"HistoNet Server" {HistoNet-at-Pathology.swmed.edu}

Lee

Lee Cohen-Gould
EM & Confocal Facilities
Weill Medical College of Cornell U.
(212)746-6146
Rms A-105, LC-207




From daemon Mon Jan 31 18:30:48 2000



From: ctschristopher :      ctschristopher-at-samiot.uct.ac.za
Date: Mon, 31 Jan 2000 17:36:52 +0300
Subject: TEM Video imaging

Contents Retrieved from Microscopy Listserver Archives
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Hi folks

The Cape Town weather is great today, beautiful clear blue skies and a nice
warm 25 C.

We have a Philips TEM 420 which has an EDAX system attached (which is
non-functional at the moment). We are looking for video / digital image
grabbing system that we can use to grab images for prints and possibly Image
analysis. Is this possible on the 420 ? Can anyone suggest a system?

Thanks

Phil


Phillip Christopher
Cardiovascular Research,UCT
Anzio Road, Observatory, 7925
27-21-4066613/6476(tel)
27-21-4485925(fax)
ctschristopher-at-samiot.uct.ac.za



From daemon Mon Jan 31 18:30:49 2000



From: Joyce Craig :      j-craig-at-CSU.EDU
Date: Mon, 31 Jan 2000 10:06:12 -0600
Subject: TEMSEM Halophytes

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A graduate student here (Rita Ware) had some success fixing halophilic
bacteria for TEM several years ago. She used the growth medium as a
buffer. She made a poster presentation at an MSA meeting.



From daemon Mon Jan 31 18:30:50 2000



From: Sara Miller :      saram-at-duke.edu
Date: Mon, 31 Jan 2000 11:54:48 -0500 (EST)
Subject: Re: Reichert Ultracut E with cryo FC 4D

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Call TekNet: 800 835-6386

On Mon, 24 Jan 2000, Marti, Jordi wrote:

} Date: Mon, 24 Jan 2000 06:46:26 -0700
} From: Marti, Jordi {jordi.marti-at-honeywell.com}
} To: 'Microscopy' {Microscopy-at-sparc5.Microscopy.Com}
} Subject: Reichert Ultracut E with cryo FC 4D
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
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} -----------------------------------------------------------------------.
}
}
} The power supply in our cryo microtome is having problems which might be
} related to the transformer. I was told by the service engineer that the
} transformer is no longer supported by Reichert. Does any one know where I
} can get a replacement ?
}
} Thanks
}
} Jordi Marti
}
}

Sara E. Miller, Ph. D.
P. O. Box 3020
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-8735



From daemon Mon Jan 31 18:30:51 2000



From: David Henriks :      Henriks-at-CompuServe.COM
Date: Mon, 31 Jan 2000 12:06:45 -0500
Subject: SEM - Manufactures of Microcleave tools for silicon wafers???

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Dear Timothy:

South Bay Technology, Inc. manufactures an SEM Cleaving System which
provides a means to precisely and quickly cleave a wafer while in the
inspection mode. A wafer is mounted to a vacuum chuck which is positioned
under an optical microscope. The exact area of interest is located
visually and the sample is cleaved at that point. SEM compatible versions
of the cleaving system are under development which will allow the user to
image and cleave while mounted in the SEM. The Cleaving System is quick,
easy to operate and precise. It allows anyone to quickly and repeatably
prepare SEM cross sections.

If you have an interest, please let me know and we can discuss your
requirements in detail.

Best regards-

David
Writing at 11:48:36 AM on 01/28/2000

***************************************************************************
************************

David Henriks
Vice President TEL: 800-728-2233
(toll free in the USA)
South Bay Technology, Inc. +1-949-492-2600
1120 Via Callejon FAX: +1-949-492-1499
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

***************************************************************************
************************

} } } } } Please visit us at http://www.southbaytech.com { { { { {

Manufacturers of precision sample preparation equipment and supplies for
metallography, crystallography and electron microscopy.


Message text written by Timothy Dimitri
}
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Hello,

I am looking for the names of any manufactures (other than SELA) that
have a product that can cleave sub micron features on silicon wafers...

Thank you

Timothy Dimitri
ASTC Failure Analysis Laboratory
IBM Microelectronics Division


{



From daemon Mon Jan 31 18:30:51 2000



From: David Henriks :      Henriks-at-CompuServe.COM
Date: Mon, 31 Jan 2000 12:06:43 -0500
Subject: TEM - Manufactures of Microcleave tools for silicon wafers???

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Timothy:

I forget to mention in my original posting that South Bay Technology also
produces the MicroCleave kit which is designed for TEM cross sectioning.
Again, if you have an interest, please let me know and I'll get you
additional information.

Best regards-

David
Writing at 11:53:19 AM on 01/28/2000

***************************************************************************
************************

David Henriks
Vice President TEL: 800-728-2233
(toll free in the USA)
South Bay Technology, Inc. +1-949-492-2600
1120 Via Callejon FAX: +1-949-492-1499
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

***************************************************************************
************************

} } } } } Please visit us at http://www.southbaytech.com { { { { {

Manufacturers of precision sample preparation equipment and supplies for
metallography, crystallography and electron microscopy.
Message text written by Timothy Dimitri
}
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Hello,

I am looking for the names of any manufactures (other than SELA) that
have a product that can cleave sub micron features on silicon wafers...

Thank you

Timothy Dimitri
ASTC Failure Analysis Laboratory
IBM Microelectronics Division

{



From daemon Mon Jan 31 18:30:51 2000



From: David Henriks :      Henriks-at-CompuServe.COM
Date: Mon, 31 Jan 2000 12:06:40 -0500
Subject: Looking for Ion Beam sputter coater or similar

Contents Retrieved from Microscopy Listserver Archives
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Dear Dr. Lartius:

While I don't have any leads on a used IBS system, I wanted to let you know
that we at South Bay Technology, Inc. are continuing the manufacture of the
IBS system formerly produced by VCR Group. Actually, we have updated the
system and are now offering the IBS/E. The IBS/E now adds the capability
of etching samples as well as coating and will accommodate samples up to 2"
in diameter.

If you would like additional information, please feel free to contact me.

Best regards-

David
Writing at 9:48:22 AM on 01/31/2000

***************************************************************************
************************

David Henriks
Vice President TEL: 800-728-2233
(toll free in the USA)
South Bay Technology, Inc. +1-949-492-2600
1120 Via Callejon FAX: +1-949-492-1499
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

***************************************************************************
************************

} } } } } Please visit us at http://www.southbaytech.com { { { { {

Manufacturers of precision sample preparation equipment and supplies for
metallography, crystallography and electron microscopy.

Message text written by "Dr. Raj Lartius"
}
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


I'm trying to find a used ion beam sputter coater or something similar...
The system I am used to is the old VCR group ion beam sputter coater. Any
leads that you might have would be greatly appreciated...

Thanks,

Raj

*********************************************
Raj Lartius, Ph.D.
NovaScan Technologies
Iowa State University Research Park
2501 North Loop Drive
Ames, Iowa USA 50010

Email: rlartius-at-novascan.com
Web: www.novascan.com
**********************************************

{



From daemon Mon Jan 31 18:30:54 2000



From: HILDEGARD CROWLEY :      hcrowley-at-du.edu
Date: Mon, 31 Jan 2000 11:06:50 -0700 (MST)
Subject: Sandbox Squabble-Weather

Contents Retrieved from Microscopy Listserver Archives
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Hi,

Are we like kids sitting in a sandbox and squabbling? Yes weather, no
weather! He said it first! She started it! I don't want weather on the
listserver. I want to read about weather in the boondocks. Who cares
about you, anyway? Sort of funny when you think of it that way, isn't it?

Bye guys,
Hildy Crowley



From daemon Mon Jan 31 18:30:57 2000



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Mon, 31 Jan 2000 08:49:42 -1000 (HST)
Subject: SEM: JEOL JSM840-AM1000

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Hi, microscopists-

A friend has the chance to buy a used JEOL JSM840 - AM1000 with EDX and
all the bells and whistles. He has asked if this is a good instrument,
and worth the price (whatever that is - he won't tell me). Since I
haven't seen the instrument and I don't know JEOLs at all, I told him I
would ask the experts.

If anyone can tell me a little about the instrument and what it might be
worth (the second part of the question being more difficult), I would
appreciate it.


In Honolulu it is gloriously clear and sunny, with temperatures near 80F
during the day and about 60F at night, which is unusually cold but really
nice.

Aloha,
Tina

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************



From daemon Mon Jan 31 18:31:10 2000



From: Steve Barlow :      sbarlow-at-sunstroke.sdsu.edu
Date: Mon, 31 Jan 2000 13:32:03 -0800
Subject: Teaching/Outreach call for papers

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You are invited to participate in a symposium of remote access microscopy,
and /or teaching microscopy to take place at the Microscopy &
Microanalysis Annual Meeting August 13-17, 2000 in Philadelphia, Pa.

Platform and poster contributions are welcome. Please contact me
directly for more information about the symposium.

Deadline for receipt of a 2page abstract is Feb 15, 2000. For
registrationand abstract forms, see
http://www.microscopy.com/MSAMeetings/MMMeeting.html

ADVANCES IN INSTRUMENTATION AND TECHNIQUES SYMPOSIUM 19: TEACHING
MICROSCOPY IN THE NEW MILLENNIUM

Organizer: Steve Barlow

The use of computers to control microscope operations, the ability to
control microscopes remotely over the Internet, and the creation of
microscope computer simulations allow researchers and students to access
microscopes in new ways. These developments mean changes in the way
microscopy can be taught to students and researchers. This symposium will
examine different ways to teach microscopy and microscope theory and
operation to reseachers and students of all levels, in the context of new
laboratory configurations, computer simulations, remote access usage, and
classroom exercises.


Dr. Steven Barlow, Associate Director
EM Facility/Biology Department
San Diego State University
5500 Campanile Drive
San Diego CA 92182-4614
phone: (619)594-4523
fax: (619) 594-5676
email: sbarlow-at-sunstroke.sdsu.edu
website: http://www.sci.sdsu.edu/emfacility/

Chairman, Educational Outreach subcommittee
promoting access to microscopes
Microscopy Society of America http://www.msa.microscopy.com/




From daemon Mon Jan 31 18:31:28 2000



From: Valerie Leppert :      vjleppert-at-ucdavis.edu
Date: Mon, 31 Jan 2000 15:43:33 -0800 (PST)
Subject: Question on Philips CM-12 SAD Alignment

Contents Retrieved from Microscopy Listserver Archives
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Hi there;

We have a new (to us) Philips CM-12 TEM in our lab and are wondering how
to get the intermediate lens focused on the diffraction aperture (for
making the first image plane and the diffraction aperture coincident
prior to obtaining a SAED pattern). It doesn't seem to be covered in the
manual.

Any help would be appreciated as this is a completely new microscope to
us.

Thanks,
Valerie Leppert



From daemon Mon Jan 31 18:31:30 2000



From: Earl Weltmer :      earlw-at-pacbell.net
Date: Mon, 31 Jan 2000 15:59:18 -0800
Subject: Re: SEM: JEOL JSM840-AM1000

Contents Retrieved from Microscopy Listserver Archives
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The JEOL 840 is an excellent instrument: very reliable & very easy to use.
Enjoy with confidence.

Earl Weltmer

P.S.: Does your friend need someone to install the SEM? I would love to install
it assuming it is in Hawaii.



Tina Carvalho wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hi, microscopists-
}
} A friend has the chance to buy a used JEOL JSM840 - AM1000 with EDX and
} all the bells and whistles. He has asked if this is a good instrument,
} and worth the price (whatever that is - he won't tell me). Since I
} haven't seen the instrument and I don't know JEOLs at all, I told him I
} would ask the experts.
}
} If anyone can tell me a little about the instrument and what it might be
} worth (the second part of the question being more difficult), I would
} appreciate it.
}
} In Honolulu it is gloriously clear and sunny, with temperatures near 80F
} during the day and about 60F at night, which is unusually cold but really
} nice.
}
} Aloha,
} Tina
}
} ****************************************************************************
} * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
} * Biological Electron Microscope Facility * (808) 956-6251 *
} * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
} ****************************************************************************



From daemon Mon Jan 31 18:31:31 2000



From: Kristen Lennon :      kalen-at-citrus.ucr.edu
Date: Mon, 31 Jan 2000 16:22:55 -0800
Subject: historesin supplier

Contents Retrieved from Microscopy Listserver Archives
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Hello All,
Does anyone know of a supplier for Historesin, formerly Cambridge, Leica,
LKB? And, the weather in SoCal is quite lovely today - it finally rained.
Thanks,
Kristen
Kristen A. Lennon
Cell, Molecular & Developmental Biology Group
Department of Botany & Plant Sciences
University of California
Riverside, CA 92521
kalen-at-citrus.ucr.edu



From daemon Mon Jan 31 20:32:35 2000



From: Chuck Butterick :      cbutte-at-ameripol.com
Date: 1/31/00 11:06 AM
Subject: Sandbox Squabble-Weather

Contents Retrieved from Microscopy Listserver Archives
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The whole weather thing has been pretty silly.

Chuck Butterick
Engineered Carbons, Inc.


______________________________ Reply Separator _________________________________


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Hi,

Are we like kids sitting in a sandbox and squabbling? Yes weather, no
weather! He said it first! She started it! I don't want weather on the
listserver. I want to read about weather in the boondocks. Who cares
about you, anyway? Sort of funny when you think of it that way, isn't it?

Bye guys,
Hildy Crowley






From daemon Tue Feb 01 07:28:29 2000



From: Ken Tiekotter :      tiekotte-at-up.edu
Date: Mon, 31 Jan 2000 23:44:54 -0800 (PST)
Subject: Re: Wanted:Used TEM for virus work

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Would you be interested in a Zeiss 9s2 TEM? 60k is the top magnification.
It has been a nifty scope as we have upgraded to a Zeiss 109. If you are
interested let me know.
Cheers!
-Ken
------------
Ken Tiekotter
Dept. of Biol.
The University of Portland
5000 Willamette Blvd.
Portland, OR 97303

On Wed, 26 Jan 2000, Cynthia Shannon wrote:

} ------------------------------------------------------------------------
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} -----------------------------------------------------------------------.
}
}
} Subject: Wanted: Used TEM for virus work
} Date: Tues, 26 Jan 2000
} } From: cshannon-at-nctimes.net
} To: Microscopy-at-sparc5.microscopy.com
}
} Does anyone have an old TEM for virus work?
} I am the electron microscopist for the county veterinarian. We are short
} of funds. Please contact me by email.
} Thanks.
} Cindy Shannon
}
}
}



From daemon Tue Feb 01 07:28:46 2000



From: Asbj¿rn Skogstad :      asbjorsk-at-stami.no
Date: Tue, 01 Feb 2000 12:17:19 +0100
Subject: LM,fluorescence - aggregation of bacteria

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-Obviously an advantage for many bacteria species, but a nightmare for
the microscopist who wants to count
them. For several reasons we want to split the aggregates of bacteria
into single cells before counting.
We have tried mild detergent treatment and ultrasound though with
limited success. The samples are initially
taken, as filter samples in working atmospheres were bacteria could be
airborne, e.g. farm work. Afterwards
the bacteria are washed off the filter, resuspended, stained (AO),
refiltered on black pc-filter, mounted and
counted. Any suggestions for a treatment, which will de-aggregate the
bacteria, are most welcome.

Asbjorn Skogstad
National Inst. of Occup. Health,
Oslo, Norway
asbjorn.skogstad-at-stami.no



From daemon Tue Feb 01 07:40:49 2000



From: Tony Bruton :      bruton-at-EMU.UNP.AC.ZA
Date: 1/31/00 11:06 AM
Subject: Sandbox Squabble-Weather

Contents Retrieved from Microscopy Listserver Archives
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Chuck 'an all

That's why I tried to bring in a useful element to it by mentioning, a little tongue in cheek, my humidity vs formvar films question. But thanks to the silly weather thread I had some useful and interesting responses. May I be forgiven for saying that there's a silver lining..............

Tony Bruton
University of Natal
South Africa

} } } "Chuck Butterick" {cbutte-at-ameripol.com} 02/01/00 04:06AM } } }
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The whole weather thing has been pretty silly.

Chuck Butterick
Engineered Carbons, Inc.


______________________________ Reply Separator _________________________________


------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America



Hi,

Are we like kids sitting in a sandbox and squabbling? Yes weather, no
weather! He said it first! She started it! I don't want weather on the
listserver. I want to read about weather in the boondocks. Who cares
about you, anyway? Sort of funny when you think of it that way, isn't it?

Bye guys,
Hildy Crowley








From daemon Sun Feb 20 13:53:24 2000



From: Walck. Scott D. :      walck-at-ppg.com
Date: Sat, 19 Feb 2000 16:42:48 -0500
Subject: DTSA Geometry setup for JEOL 2000FX in Experimental Header(Kevex

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Quantum)


Does anyone have the geometry settings for a Kevex Quantum detector on a
JEOL 2000FX? I am using DTSA and need the values. I would like to have the
sample to detector distance and the azimuthal angle. I am using +45 for the
azimuthal angle and 90 for the detector angle. Are these correct? I know
that the takeoff angle is 70.
-Scott


Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)




From daemon Sun Feb 20 13:53:24 2000



From: David Lockwood :      d.lockwood-at-mailbox.uq.edu.au
Date: Sun, 20 Feb 2000 09:13:45 +1000
Subject: Fluoro - GFP, Nikon filters, fixation

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Hi all. I have just joined this list and am posting without the usual
"lurking time" due to time constraints - please forgive if recently
covered. I am also a novice in all microscopy techniques, especially
fluorescence.

I am trying to detect GFP-containing cells in liver tissue and having
some problems.

1. I can't determine the spectra for the filter blocks I am using (on a
Nikon Eclipse TE300 with TE-FM Epifluorescence attachment). The blocks
are marked "UV", "B-2" (blue from source, green in field) and "G" (green
from source, red in field). I have e-mailed Nikon and to be fair
they've only had a few days but I'm under some pressure. No help from
the manual. I have been using B-2 for GFP.

2. I get quite a lot of background fluorescence even with frozen
sections (fixed in neutral buffered formalin). Can anyone suggest a way
to reduce this? (eg any extra filters?)

3. I have read conflicting opinions on fixation. Most previous work has
used thick sections (50 microns cut eg with Vibratome, ? to avoid having
to embed tissue blocks). GFP is interfered with by acetone (and probably
other organic solvents) but I would have thought that after fixation
with formalin it should be stabilized and resistant to the
xylene/alcohol used in paraffin embedding. I have seen fluorescence
retained after this treatment but perhaps it can be improved.

4. For those with liver fluoro experience - on "UV" setting I see bright
fluorescence which photobleaches. I believe I am looking at retinoids in
stellate cells, although the bleaching is incomplete and a bit slower
than I have seen before. Can anyone confirm this?

Apologies for long post. Any help much appreciated.

David Lockwood
University of Qld Dept of Surgery
PA Hospital Brisbane Australia



From daemon Fri Feb 11 18:29:51 2000



From: Marty Reed :      mmr7001-at-humboldt.edu
Date: Fri, 04 Jan 1980 08:23:24 -0800
Subject: questions for MSA members

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A colleague would like your responses to these questions. I believe that I
say something on the first question about a year ago and apologize for
asking again.


} Here's what I/we need to know.
}
} 1) Are Microstar diamond knives equal to Diatome. We have an offer that
} will save us about $1200 on three knives ( one 3mm ultra with 45 degree
} angle, one 3mm ultra with 35 degree angle, one 10 mm histo knife).
}
}
} 2) Is the power controller feature on the Pelco Microwave Tissue processor
} (model #3451) worth it? (costs about $1300 additional compared to the
} Pelco 3450 without the power controller). I plan to do immunolocalization
} work, but am not sure this power controller feature is necessary or worth
} the cost.
}
} Casey
}
}
}
Thanks for your time
}
Marty Reed
Equipment Technician
Biology Department
Humboldt State University
Arcata CA 95521
707-826-3234
707-826-3201 FAX
mmr7001-at-axe.humboldt.edu







From daemon Tue Feb 01 10:56:26 2000



From: Dorota Wadowska :      wadowska-at-upei.ca
Date: Tue, 01 Feb 2000 10:58:08 -0400
Subject: TEM H600

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Hi!
Could anybody tell me what would be the sale price for a Hitachi
H600?
Thanks
Dorota



From daemon Tue Feb 01 10:56:32 2000



From: Walck. Scott D. :      walck-at-ppg.com
Date: Tue, 1 Feb 2000 11:28:02 -0500
Subject: RE: Question on Philips CM-12 SAD Alignment

Contents Retrieved from Microscopy Listserver Archives
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I don't use a CM series microscope on a regular basis, but what is important
is that you set it up the same way every time.

You can not make the back focal plane of the objective lens coincident with
the objective aperture. The back focal plane is well above the location of
the objective aperture plane.

There are two ways that I use to set up diffraction patterns reproducibly
depending on whether I am using CBED or SAD. Both are set up after the
sample has been made eucentric and the image focussed.

CBED: This method can be done for both CBED and SAD. The shadow of the
condenser aperture defines the diameter of the diffraction disk. When the
intermediate lens is adjusted properly, the edges of the diffraction disks
will be in focus. You are grabbing the back focal plane for your
diffraction pattern in the projector lens system. You will note that all of
the HOLZ lines (if you can see them) are the sharpest at this condition. If
you have a highly polycrystalline sample with continuous diffraction rings,
this method is difficult to do.

SAD. Spread the beam with the condenser all the way. (clockwise in the
CM-12 will go to more parallel beam faster than CCW -I think.) Then focus
the spot to the smallest that you can. You can take a really long exposure
or cheat a little and put some intensity back into the pattern with the
condenser lens. You will note that the objective aperture is not focused in
this method.

The most important thing to remember is to make the sample eucentric and
focus before during either of these methods and to do the diffraction
focussing consistently from sample to sample.
-Scott


Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)


"The opinions expressed are those of Scott D. Walck and not of PPG
Industries, Inc. nor of any PPG-associated companies."
--



} -----Original Message-----
} From: Valerie Leppert [mailto:vjleppert-at-ucdavis.edu]
} Sent: Monday, January 31, 2000 6:44 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Question on Philips CM-12 SAD Alignment
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} ---------.
}
}
} Hi there;
}
} We have a new (to us) Philips CM-12 TEM in our lab and are
} wondering how
} to get the intermediate lens focused on the diffraction aperture (for
} making the first image plane and the diffraction aperture coincident
} prior to obtaining a SAED pattern). It doesn't seem to be
} covered in the
} manual.
}
} Any help would be appreciated as this is a completely new
} microscope to
} us.
}
} Thanks,
} Valerie Leppert
}
}



From daemon Tue Feb 01 11:47:12 2000



From: Laurie Wallin :      lwallin-at-ucsd.edu
Date: Tue, 1 Feb 2000 09:02:04 -0800
Subject: Any other fluorescence microscopy classes?

Contents Retrieved from Microscopy Listserver Archives
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I received Simon Watkins' note about the microscopy class in Maine and
wonder if anyone knows about similar courses closer to California in the
near future? We've got a number of technicians working on fluorescence
microscopy in our lab, and I think it would be great to have some more
formal training for us!

Sincerely,
Laurie Wallin
UCSD Department of Anesthesiology
9500 Gilman Drive, 0629, La Jolla, CA 92093



From daemon Tue Feb 01 13:58:38 2000



From: Robert S Dotson :      rdotson-at-mailhost.tcs.tulane.edu
Date: Tue, 1 Feb 2000 11:55:19 -0600 (CST)
Subject: used Philips EM410

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We are disposing of our old Philips 410 transmission
electron microscope. It is in pretty good shape but
is not fully functioning. At minimum it needs its ion
getter reconditioned. Does anyone know of someone who
might want to buy it and fix it up or use it for
parts?
-Robert


____________________________________

Robert S. Dotson, Ph.D.,
Laboratory Supervisor, Microscopy

Coordinated Instrumentation Facility
605 Lindy Boggs Building
Tulane University
6823 St. Charles Avenue
New Orleans, LA 70118-5698

504-865-5142| fax 504-865-6768

rdotson-at-mailhost.tcs.tulane.edu
http://www.tulane.edu/~cif
____________________________________



From daemon Tue Feb 01 13:58:42 2000



From: best-at-Juniata.Edu
Date: Tue, 1 Feb 2000 14:09:48 -0500
Subject: RE: weather & Formvar films

Contents Retrieved from Microscopy Listserver Archives
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I am thankful to those who took this weather thread and imparted
some information that I can really use. The retired professor that taught
me how to release forvar films had no idea why sometimes it worked and
sometimes it didn't. Now at least I have a couple of likely variables to
check.

Thanks!
Chris Best
Mol. Biol.
Juniata College
Huntingdon, PA 16652


PS - I can't help but be amused that even on a forum for scientists,
the petty &/or silly items get the most responses. Tell the truth, do you
stay up at night watching Jerry Springer? (For heaven sake, don't answer
that!)




From daemon Tue Feb 01 14:23:32 2000



From: Alwyn Eades :      jae5-at-lehigh.edu
Date: Tue, 01 Feb 2000 15:01:57 -0500
Subject: SAD and the back focal plane

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I am afraid that I do not agree with Scott Walck about back focal planes.
I do not have a CM 12 in the lab here. So I can not check that I am not
confusing the CM 12 with other Philips/FEI instruments. However, when
Scott says “You can not make the back focal plane of the objective lens
coincident with the objective aperture. The back focal plane is well above
the location of the objective aperture plane.” he is wrong. On all
microscopes except the very few which have very small pole-piece gaps for
high resolution, the objective aperture should coincide with the back focal
plane.

There is an easy and accurate way to find the true back focal plane on a CM
12 (or any other microscope with an immersion lens). Use a crystalline
sample, go to convergent-beam diffraction then use the diffraction focus to
make the Kikuchi lines as sharp as you can. That is the back focal plane.

If the microscope is set up properly, the image of the objective aperture
should be sharply in focus at nearly the same setting of the diffraction
focus. If the diffraction focus to give a sharp image of the objective
aperture is very different from the diffraction focus to make the Kikuchi
lines sharp, get your service engineer to reset the height of the objective
aperture until they agree.

If the sample is at the correct eucentric height, a selected-area
diffraction pattern in the true back focal plane will have sharp spots for
a C2 setting almost but not quite to the maximum (almost fully clockwise).
Again, if it does not, ask the service engineer to fix it.



Alwyn Eades
Department of Materials Science and Engineering
Lehigh University
5 East Packer Avenue
Bethlehem
Pennsylvannia 18015-3195
Phone 610 758 4231
Fax 610 758 4244
jae5-at-lehigh.edu



From daemon Tue Feb 01 15:14:30 2000



From: Walck. Scott D. :      walck-at-ppg.com
Date: Tue, 1 Feb 2000 15:58:05 -0500
Subject: RE: SAD and the back focal plane

Contents Retrieved from Microscopy Listserver Archives
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Alwyn,
We have a CM-12 at our other facility and I will check it out in a day or
so.

I thought that I did and it worked like our JEOL 2000FX. In the 2000FX,
because the lens is highly excited, there are 3 cross overs in the objective
lens after the sample. That means the true back focal plane is inside the
objective lens and it is not possible to put the aperture at that plane. In
the 2000FX, I have focused the CBED pattern and have found the objective
aperture not in focus. I have also found that the two methods that I
outlined do not agree with the camera constants. The 2000FX has a condenser
mini lens to make the beam parallel, but the highly excited lens allows the
small probes. Since the CM-12 can form the small probes, I thought that the
lens system was working in a similar manner.

I will try it out unless someone beats me to it. How about it CM owners?

-Scott


Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)



} -----Original Message-----
} From: Alwyn Eades [mailto:jae5-at-lehigh.edu]
} Sent: Tuesday, February 01, 2000 3:02 PM
} To: microscopy-at-sparc5.microscopy.com
} Subject: SAD and the back focal plane
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} ---------.
}
}
}
} I am afraid that I do not agree with Scott Walck about back
} focal planes.
} I do not have a CM 12 in the lab here. So I can not check
} that I am not
} confusing the CM 12 with other Philips/FEI instruments.
} However, when
} Scott says "You can not make the back focal plane of the
} objective lens
} coincident with the objective aperture. The back focal plane
} is well above
} the location of the objective aperture plane." he is wrong. On all
} microscopes except the very few which have very small
} pole-piece gaps for
} high resolution, the objective aperture should coincide with
} the back focal
} plane.
}
} There is an easy and accurate way to find the true back focal
} plane on a CM
} 12 (or any other microscope with an immersion lens). Use a
} crystalline
} sample, go to convergent-beam diffraction then use the
} diffraction focus to
} make the Kikuchi lines as sharp as you can. That is the
} back focal plane.
}
} If the microscope is set up properly, the image of the
} objective aperture
} should be sharply in focus at nearly the same setting of the
} diffraction
} focus. If the diffraction focus to give a sharp image of
} the objective
} aperture is very different from the diffraction focus to make
} the Kikuchi
} lines sharp, get your service engineer to reset the height of
} the objective
} aperture until they agree.
}
} If the sample is at the correct eucentric height, a selected-area
} diffraction pattern in the true back focal plane will have
} sharp spots for
} a C2 setting almost but not quite to the maximum (almost
} fully clockwise).
} Again, if it does not, ask the service engineer to fix it.
}
}
}
} Alwyn Eades
} Department of Materials Science and Engineering
} Lehigh University
} 5 East Packer Avenue
} Bethlehem
} Pennsylvannia 18015-3195
} Phone 610 758 4231
} Fax 610 758 4244
} jae5-at-lehigh.edu
}
}



From daemon Wed Feb 02 17:01:23 2000



From: Henry Eichelberger :      heichelb-at-binghamton.edu
Date: Tue, 1 Feb 2000 16:14:07 -0500
Subject: RE: Reichert Ultracut parts & repair

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-----Original Message-----



Jordi:
For Reichert repair and parts I would suggest Helmut Patzig of MOC
(Microscopical Optical Consulting Inc.), phone (914) 268-6450 or e-mail
MOCLeica-at-Aol.com Helmut is known to carry spare parts for Reichert and LKB
ultramicrotomes.
I have no vested interest in MOC other than as a satisfied customer.
If he cannot help, you could ask him what your Reichert transformer voltage
output should be and using a voltage meter adjust the voltage of a variable
voltage transformer to the correct amount. Make sure to incorporate a
"stop" on the dial so the voltage can't be accidentally moved above the
correct amount.
Henry


Henry Eichelberger, Manager
Electron Microscopy Facility
Department of Biological Sciences
Binghamton University
Binghamton, NY 13902-6000

phone: (607) 777-2682
fax: (607) 777-6521
e-mail: heichelb-at-binghamton.edu


-----Original Message-----
------------------------------------------------------
On Mon, 24 Jan 2000, Marti, Jordi wrote:

} The power supply in our cryo microtome is having problems which might be
} related to the transformer. I was told by the service engineer that the
} transformer is no longer supported by Reichert. Does any one know where I
} can get a replacement ?
}
} Thanks
}
} Jordi Marti




From daemon Wed Feb 02 17:02:00 2000



From: Milo Kral :      m.kral-at-mech.canterbury.ac.nz
Date: Wed, 02 Feb 2000 15:28:32 +1300
Subject: Looking for a turbo pump

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Does anyone have an extra turbo pump that they can part with?

the weather is quite wintery considering its summer here in Christchurch
New Zealand. low clouds with southerly prevailing winds (explaining the
COLD)




From daemon Wed Feb 02 17:02:10 2000



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Wed, 02 Feb 2000 02:37:10 -0500
Subject: Silver membranes being discontinued

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-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Hello,

I apologize in advance if this posting offends anyone. However there are a
good many people who use silver membranes in their work and to have the
supply from the worlds only manufacturer (to my knowledge) come to a halt,
has the potential of being highly disruptive at least to some programs:
=================================================
Osmonics has announced the closing of our Phoenix, AZ manufacturing facility
effective 1 May 2000. Since our silver membranes are manufactured in this
facility, Osmonics has decided to end production of this membrane because of
declining sales and the very expensive costs associated with moving the mfg.
. plant to another location. All orders placed before 1 March, 2000 will be
honored and filled.
==================================================
We plan to make a "last buy" before the cut off date of March 1, 2000. I
would advise anyone depending on these silver membranes for their work to
take stock of their future requirements because after the cut off date,
sales will be possible only from remaining stocks.

For those who do run out, and are in a bind, we are prepared help them find
alternative filtration media, of which there are indeed some alternatives
for some applications.

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.spi.cc
########################
============================================



From daemon Wed Feb 02 17:02:13 2000



From: Keith Ryan :      KPR-at-wpo.nerc.ac.uk
Date: Wed, 02 Feb 2000 08:35:01 +0000
Subject: LM - Cryostat - marine larvae

Contents Retrieved from Microscopy Listserver Archives
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Hello Listers

I have someone here who wants to freeze and cut cryostat sections of
marine larvae (5 microns?) for immuno/light microscopy. The specimens
are 100-200 microns in length.

1. What would be the best way of handling these small items?
2. What would be the best support/medium e.g TissueTek?
3. What about cryoprotection? I am familiar with 2.3 molar sucrose
for EM.
4. Any general tips for immuno (protein/amino peptidases)?

Thanks - Keith
_______________________
Keith Ryan (Dr)
Marine Biological Association
Citadel Hill
Plymouth
Devon PL1 2PB
England

Tel. 0044 (0)1752 633279 (with answer machine)
also 0044 (0)1752 633249
Fax. 0044 (0)1752 633102

e-mail: kpr-at-wpo.nerc.ac.uk



From daemon Wed Feb 02 17:02:11 2000



From: Ron Doole :      ron.doole-at-materials.oxford.ac.uk
Date: Wed, 2 Feb 2000 08:38:24 +0000 (GMT Standard Time)
Subject: re - CM12 SAD focus

Contents Retrieved from Microscopy Listserver Archives
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Hi Valerie,

Like the other respondees I am not a CM12 user
(where are they?), however, as an ex CM12 user of some 10
years ago I think that I remember the alignment that Valerie
is asking about. In the basic alignment of the machine there
was a step during which the SAD aperture was focussed. I
think that it was in the `service calibrations' page. This
may have changed in later software revisions.

The alignment of this microscope was usually
carried out by the engineer when the instrument was
installed. They would set up the objective lens current and
adjust the specimen goniometer height to ensure that when
it was at the eucentric position it was correctly in focus.
Following this a complete column alignment was carried out
including focussing the SAD aperture. The manufacturers
then assumed that the operator would set up the specimen to
the eucentric height, it should focus in the same position
and the aperture should be in focus.

If the aperture is not in focus when in the SA
range (as noted next to the mag readout) then check you are
correctly at the eucentric position, if you are then either
it was not aligned properly after installation or something
has changed.
NOTE: DO NOT ADJUST THE SERVICE ALIGNMENTS UNLESS YOU KNOW
WHAT YOU ARE DOING AS YOU CAN LOSE THE INSTRUMENT
ALIGNMENTS.

Good luck,
Ron


----------------------
Mr. R.C. Doole
Department of Materials,
University of Oxford.
Parks Road, Oxford. OX1 3PH. UK.
Phone +44 (0) 1865 273701
Fax +44 (0) 1865 283333
ron.doole-at-materials.ox.ac.uk



From daemon Wed Feb 02 17:02:14 2000



From: Jonathan Barnard :      Jonathan.Barnard-at-Angstrom.UU.SE
Date: Wed, 02 Feb 2000 11:20:15 +0100
Subject: TEM:finding the parallel beam

Contents Retrieved from Microscopy Listserver Archives
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I got a tip for finding the condenser lens settings required for parallel
illumination from Angus Kirkland (Cambridge).

Make sure you are in microprobe mode and set up for SAED and the specimen
is out of the way. Spread the beam, put in the SA Aperture, go to
diffraction and then sweep the SA aperture (SAA) from side to side and
minimise the deflection of the diffraction spot by tweaking the
illumination control (C2 or second condenser lens). A little bit of thought
will convince you that anything other than a parallel beam will give you a
side to side motion (tilt) when the SAA is swept.

I have found it useful to keep a table of C2 condenser lens current
settings for each spot size (C1 excitation) in microprobe mode. Setting the
C2 lens for parallel illumination and adjusting the diffraction focus to
get the sharpest spot will then give you near perfect SAD conditions.

Anyone contemplating electron holography for example, will have to start
from the parallel illumination to get the best spatial coherence for their
holograms.

********************************************************
Dr Jonathan Barnard

Analytical Materials Physics
The Angstrom Laboratory, Uppsala University
P O Box 534, SE-751 21 Uppsala, Sweden
Phone: +46-(0)18-4716838 Fax: +46-(0)18-500131
http://www.angstrom.uu.se/analytical/home.html

********************************************************



From daemon Wed Feb 02 17:02:20 2000



From: Matt_Plantinga-at-amway.com
Date: Wed, 2 Feb 2000 08:15:48 -0500
Subject: LSCM Need help with non-fluorescing resin

Contents Retrieved from Microscopy Listserver Archives
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I am working on fluorescent imaging of thin sections cut from samples
embedded in TEM embedding resin, and am having a significant problem with
resin fluorescence. Does anyone know of an embedding resin that doesn't
autofluoresce?

Matt Plantinga
Amway Corporation
matt_plantinga-at-amway.com



From daemon Wed Feb 02 17:03:28 2000



From: Andrew Ochalski :      aochalsk-at-science.uottawa.ca
Date: Wed, 2 Feb 2000 14:05:21 GMT+5
Subject: Heating stage with DvorakStotler Chamber...Recommendations?

Contents Retrieved from Microscopy Listserver Archives
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{color} {param} 0100,0100,0100 {/param} {FontFamily} {param} Arial {/param} {smaller} Fellow Microscopists,


We have a Zeiss Axiophot microscope and a
Dvorak-Stotler Chamber that we would like to
assemble into a temperature-controlled environment
for digital live-cell imaging. I have several questions
about the best way to combine these elements:

i) Can anyone recommend a heating stage
compatible with both the microscope and the
chamber?

ii) Would the best position for a temperature sensor
be on the top (near the objective?) or on the bottom
of the chamber, or both (two sensors)?

iii) Is it better to heat the media/fluids in their
containers, or to have an in-line heater ( brand
recommendations?)

iv) Are there any fuid flow or temperature
considerations specific to this chamber that need to
be addressed (gravity vs. pump feed, shear forces,
heating stage redundant, etc.)?

v) Are there any problems related to objective lens
mag/NA that need to be overcome when using this D-
S apparatus?


Any references, anecdotes, or words of wisdom
would be appreciated. Dealers, if you have a product
that you think I could use, please don't hesitate to
contact me. Thank you in advance. {FontFamily} {param} BrushScript BT {/param} {bigger}

{nofill}
Regards,

Andrew Ochalski,
Microscopy Technician,
Dept. of Biology,
University of Ottawa
Room 108, Gendron Bldg.
130 Louis Pasteur,
Ottawa, Ontario
CANADA
K1N 6N5
613-562-5800 x6343
FAX: 613-562 5486

{/x-rich}



From daemon Wed Feb 02 17:02:50 2000



From: Cheri Owen :      cowen-at-cmb.biosci.wayne.edu
Date: Wed, 02 Feb 2000 09:32:01 -0500
Subject: Looking for a new microscope

Contents Retrieved from Microscopy Listserver Archives
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I would like to get some information from some of the top companies on
microscopes with photographic and possibly fluourescent capabilities.
Please email me or call 313-993-4195
Thank you

Cheri Owen
Wayne State University



From daemon Wed Feb 02 17:02:48 2000



From: BARRY SHAW :      Barry.Shaw-at-nottingham.ac.uk
Date: Wed, 2 Feb 2000 14:36:36 GMT0BST
Subject: Re: Any other fluorescence microscopy classes?

Contents Retrieved from Microscopy Listserver Archives
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Hello All,

Not really a reply but another question!!
Anybody know of a similar course a little further east - England
(Great Britain) to be exact !!?
Thanks in Advance,

Baz


Barry Shaw
Senior Imaging Technician
Molecular & Cell Biology
E Floor
School Of Biomedical Sciences
University of Nottingham Medical School
NG7 2UH



From daemon Wed Feb 02 17:02:49 2000



From: Manfred Prantl :      prantl-at-alicona.com
Date: Wed, 02 Feb 2000 15:42:52 +0100
Subject: SEM and 3D?

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We are a company that has specialized on extracting 3D information
from stereoscopic SEM images. Currently we try to figure out the
potential applications and the future prospects of such techniques for
the microscopy community.

What we do exactly is that we take two images from a sample on the
SEM from slightly different tilt angles and use digital image processing

to compute a 3D elevation model. From there you can then perform
various analysis steps like extraction of 3D profiles, determination
of surface roughness etc.

My questions now are:

1.) How would you judge the importance of obtaining 3D data from a SEM
image?
2.) What would be the major requirements for such a data set to be
useful
(like density, accuracy, number of false alarms, etc.)?
3.) Is it of importance to you that you get the 3D data and the image
data
together and not separated (like it is the case with AFM)?
4.) How would you judge the future prospects and influence of such a
technique on your personal field of work?
5.) Do you generally trust the measurement of 3D data via stereoscopic
images?
6.) Do you already have experience with stereoscopic depth measurments
and what are your conclusions?

Best regards, Manfred Prantl
R&D
Alicona GmbH, Germany
www.alicona.com



From daemon Wed Feb 02 17:02:53 2000



From: Russell E. Cook :      cook-at-horus.et.anl.gov
Date: Wed, 2 Feb 2000 09:15:18 -0600
Subject: Back focal plane of CM's

Contents Retrieved from Microscopy Listserver Archives
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Irene Piscopo of FEI/Philips probably can answer your question. I'm copying
this message to her although if she's in the "field" a response may take a
few days. Also, Max Otten of FEI/Philips is another good source for that
type of information.

We have a CM-120 so we're aware of your problems in deciphering the
operator's manual. Good luck!

Bruce F. Ingber, Biologist
USDA-ARS, SRRC
1100 Robert E. Lee Blvd.
New Orleans, LA 70124

(504) 286-4270 phone
(504) 286-4419 fax
bingber-at-nola.srrc.usda.gov

-----Original Message-----
} From: Valerie Leppert [mailto:vjleppert-at-ucdavis.edu]
Sent: Monday, January 31, 2000 5:44 PM
To: Microscopy-at-sparc5.Microscopy.Com


I can't speak about Philips CM12's, but the back focal plane of the
objective coincides with the objective aperture in the CM30 here.

----------------------------------------------------------------------
Russell E. Cook, Ph.D.
Electron Microscopy Center for Materials Research
Argonne National Laboratory
Materials Science Division, Building 212
9700 South Cass Avenue
Argonne, IL 60439-4838
(630)252-7194
FAX: (630)252-4289
cook-at-horus.msd.anl.gov




From daemon Wed Feb 02 17:03:04 2000



From: Piscopo, Irene :      IPiscopo-at-FEICO.COM
Date: Wed, 2 Feb 2000 07:59:45 -0800
Subject: RE: Question on Philips CM-12 SAD Alignment

Contents Retrieved from Microscopy Listserver Archives
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Hello all: Valerie Leppart, you can reach me at 203-853-3256. I cannot call
you since there is no phone number to contact. Irene Piscopo

The CM 12 microscope has four mag ranges: LM, M, SA, Mh

As long as you are eucentric and using the SA range the image plane
and the diffraction aperture plane will be in focus. (It is done
automatically by the instrument.) This will give you accurate SAD down to
1um. The objective mag in the plane of the diffraction aperture is
approxmately 27X. If you wish to obtain diffraction from areas smaller than
one micron, use uD, or uuD. If you call me I will discuss these methods
with you and send you detailed instructions on using these methods.


Irene Piscopo


} -----Original Message-----
} From: Ingber, Bruce F. [SMTP:bingber-at-commserver.srrc.usda.gov]
} Sent: Wednesday, February 02, 2000 10:14 AM
} To: Microscopy-at-MSA. Microscopy. com (E-mail)
} Cc: Piscopo Irene (CS) (E-mail); Max T. Otten (E-mail)
} Subject: FW: Question on Philips CM-12 SAD Alignment
}
} Irene Piscopo of FEI/Philips probably can answer your question. I'm
} copying
} this message to her although if she's in the "field" a response may take a
} few days. Also, Max Otten of FEI/Philips is another good source for that
} type of information.
}
} We have a CM-120 so we're aware of your problems in deciphering the
} operator's manual. Good luck!
}
} Bruce F. Ingber, Biologist
} USDA-ARS, SRRC
} 1100 Robert E. Lee Blvd.
} New Orleans, LA 70124
}
} (504) 286-4270 phone
} (504) 286-4419 fax
} bingber-at-nola.srrc.usda.gov
}
} -----Original Message-----
} From: Valerie Leppert [mailto:vjleppert-at-ucdavis.edu]
} Sent: Monday, January 31, 2000 5:44 PM
} To: Microscopy-at-sparc5.Microscopy.Com
} Subject: Question on Philips CM-12 SAD Alignment
}
} ------------------------------------------------------------------------
} The Microscopy ListServer-Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe-Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi there;
} We have a new (to us) Philips CM-12 TEM in our lab and are wondering how
} to
} get the intermediate lens focused on the diffraction aperture (for making
} the first image plane and the diffraction aperture coincident prior to
} obtaining a SAED pattern). It doesn't seem to be covered in the manual.
} Any help would be appreciated as this is a completely new microscope to
} us.
} Thanks,
} Valerie Leppert



From daemon Wed Feb 02 17:03:59 2000



From: rgriffin-at-eng.uab.edu
Date: Wed, 2 Feb 2000 14:15:55 -0600
Subject: Gray level

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How many gray levels can the human eye distinguish? We've found several
references that disagree.
If anyone knows of a reference - that would be best.


Robin Griffin
UAB



From daemon Wed Feb 02 17:04:02 2000



From: Hendrik O. Colijn :      colijn.1-at-osu.edu
Date: Wed, 02 Feb 2000 16:07:50 -0500
Subject: Re: Gray level

Contents Retrieved from Microscopy Listserver Archives
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Robin,

I don't have the reference right in front of me, but I believe that
Gonzalez and Wintz say that

1. The eye can respond to an intensity range of ~10^10 in light intensity,
but this is the adaptive response. The perceived brightness is a log (some
will argue power law) function of the incident intensity.

2. In any one point in an image, the eye can distinguish at most ~20-30
gray levels... But... in a complex image you need at least 100 gray levels
for the eye to see it as smooth. In other words, the eye seems to adapt as
it scans the image.

Cheers,
Henk


At 02:15 PM 2/2/00 -0600, rgriffin-at-eng.uab.edu"-at-sparc5.Microscopy.Com wrote:

} How many gray levels can the human eye distinguish? We've found several
} references that disagree.
} If anyone knows of a reference - that would be best.
}
}
} Robin Griffin
} UAB

Hendrik O. Colijn colijn.1-at-osu.edu
Campus Electron Optics Facility Ohio State University
(614) 292-0674 http://web.ceof.ohio-state.edu
Murphy's Law: "If anything can go wrong, it will."
Commentary: "Murphy was an optimist."



From daemon Wed Feb 02 18:52:13 2000



From: Valerie Leppert :      vjleppert-at-ucdavis.edu
Date: Wed, 2 Feb 2000 15:04:41 -0800 (PST)
Subject: Re: re - CM12 SAD focus

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Thanks for your reply and everyone else's reply regarding the SAD
alignment. There seem to be a lot of different opinions about how this
done!

We did set the sample at the eucentric and did notice that the SAD
aperture is not quite in focus when in image mode. Of course this will
cause the diffraction information to come from an area that is displaced
with respect to the aperture position.

I am accustomed to being able to independently focus the intermediate lens
on the SAD aperture, and then bring the image into focus using the
objective lens - making the image plane and the SAD aperture coincident.
This is on a much older Philips model and a much older Hitachi model.

However, on the CM-12, there doesn't seem to be a way for the user to do
this in normal operation. I've tried a few of the suggestions (haven't
worked my way through them all yet!) with no luck yet.

It does seem that this adjustment has been grouped under the category of
"service alignment" in newer models.

Thank you to everyone who has replied.

Valerie Leppert




From daemon Wed Feb 02 18:52:14 2000



From: Karl Hagglund-KW :      hagglund.kw-at-pg.com (by way of Nestor J. Zaluzec)
Date: Wed, 2 Feb 2000 17:06:39 -0600
Subject: LM Sony camera bayonet mount

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We have a sony DXC-760 MD ccd camera that we have mounted to a standard camera
lens with a bayonet adapter. Unfortunately, we have borrowed the adapter and
have to return it. Is there a standard size (i.e. Nikon, Minolta, Canon) lens
adapter that works with this camera mount? We can't find a part number or
description for the adapter, and the book doesn't describe the size. We have
already ordered and returned one that didn't fit.

Thanks in advance.

Karl Hagglund
Proctor and Gamble
Food and Beverage Analytical Microbiology




From daemon Wed Feb 02 18:52:16 2000



From: Dr Eric Lachowski :      che136-at-abdn.ac.uk (by way of Nestor J. Zaluzec)
Date: Wed, 2 Feb 2000 17:14:03 -0600
Subject: Electron diffraction simulations

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Fellow Listers,
Does anyone know of a (preferrably free) software package
which simulates ray paths, including the diffraction mode,
in the TEM? We would like to use such a package to teach
physics students a bit of optics.

Thanks,
Eric



----------------------
Dr Eric Lachowski
Department of Chemistry
University of Aberdeen
Meston Walk
Old Aberdeen AB24 3UE
Scotland
+44 (0)1224 272934 fax 272921
e.lachowski-at-abdn.ac.uk




From daemon Wed Feb 02 18:52:20 2000



From: Paul Webster :      pwebster-at-mailhouse.hei.org
Date: 02 Feb 00 16:29:17 -0800
Subject: RE: LSCM Need help with non-fluorescing resin

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------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Reply to: RE: LSCM Need help with non-fluorescing resin
Dear Matt,

Try any of the immunocytochemical resins (Lowicryl, LR White or Gold, Unicryl etc). When specific antibodies are used on embedded tissue, they produce very beautiful fluorescent patterns that look like line drawings.

Paul Webster

Matt_Plantinga-at-amway.com wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
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} -----------------------------------------------------------------------.
}
}
} I am working on fluorescent imaging of thin sections cut from samples
} embedded in TEM embedding resin, and am having a significant problem with
} resin fluorescence. Does anyone know of an embedding resin that doesn't
} autofluoresce?
}
} Matt Plantinga
} Amway Corporation
} matt_plantinga-at-amway.com
}
}
}
}
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Paul Webster, Ph.D
House Ear Institute
2100 West Third Street
Los Angeles, CA 90057
phone:213 273 8026
fax: 213 413 6739
e-mail: pwebster-at-hei.org
http://www.hei.org/htm/aemi.htm



From daemon Wed Feb 02 22:19:11 2000



From: DrJohnRuss-at-aol.com
Date: Wed, 2 Feb 2000 19:56:04 EST
Subject: Re: Gray level

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In a message dated 2/2/00 4:00:26 PM,
rgriffin-at-eng.uab.edu-at-sparc5.Microscopy.Com writes:

} How many gray levels can the human eye distinguish? We've found several
}
} references that disagree.
}
} If anyone knows of a reference - that would be best.

Most of the books on image processing say the same thing (probably all copied
from each other and other non-prime sources), but I don't know of an original
reference based on real research. You can find the same ideas in books like
Frisby "Seeing: illusion, brain and mind", Oxford, 1980; Marr "Vision",
Freeman, 1982; and Rock "Perception", Freeman, 1984 which deal with the
human visual system not in the context of computer graphics. These are
probably closer to the original research.

The basic idea is that it takes about a 2-2.5% change in absolute brightness
(i.e. a ratio of 1.025) to be just detectable, and only if it is a fairly
sudden change spatially (or temporally for that matter). That is where the
20-30 distinct brightness levels over the full range of brightness that can
be seen at one time (i.e. without accommodations like varying pupil size)
comes from. The requirement that an entire scene have about 100 levels to
avoid banding is more controversial, based on the idea that the eye can
perform some accommodation as it traverses from one part of a scene to
another. This probably doesn't apply when (e.g.) looking through a
microscope, so the 20-30 number would be more applicable. It also ignores
color, which complicates things further.



From daemon Wed Feb 02 22:19:14 2000



From: special123-at-smartportfolio.com
Date: Thu, 3 Feb 2000 12:17:58 +0900
Subject: TEM:finding the parallel beam

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Dear Jonathan,

} From what is my understanding of optics - this will not give you parallel
illumination at the specimen plane (if this what you meant?).
By tweaking C2 you are moving the crossover plane (which is actually the
diffraction plane). You can in the same way make the spot not to move by
changing the diffraction focus. For each setting of C2 you can find the
crossover by changing the diff. focus.
Try this - spread the beam, go to diffraction, focus by diffraction focus
for smallest spot, go back to imaging mode put the SAA, go to diffraction
and try the procedure you described. I think the spot will not move.
Ofcourse if you expand the beam too much you can no longer produce small
diffraction spot (without the use of SAA) because you go out of paraxial
mode.
The diffraction plane is always the crossover plane not the theoretical back
focal plane of the objective. Its z-position changes with the change of the
C2 excitement.
The spatial coherency of the electron waves is very high (actually the
electrons are flying one by one through the microscope). The limiting
factors are the source size, the illumination angle and the energy spread.

Please correct me if I'm wrong ... I'm still "green" in electron microscopy
.. I'll be happy to learn from experienced users.

Best wishes,

Rado

---------------------------------------------------------------------
Radostin Danev
Laboratory of Ultrastructure Research
National Institute for Physiological Sciences
Myodaiji-cho, Okazaki 444-8585, JAPAN
e-mail: rado-at-nips.ac.jp
---------------------------------------------------------------------
----- Original Message -----
} From: Jonathan Barnard {Jonathan.Barnard-at-Angstrom.UU.SE}
To: {microscopy-at-sparc5.microscopy.com}
Sent: Wednesday, February 02, 2000 7:20 PM


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From daemon Thu Feb 03 15:56:54 2000



From: Dmitri V. Sokolov :      sokolov-at-ryouko.rciqe.hokudai.ac.jp
Date: Thu, 3 Feb 2000 20:20:06 +0900
Subject: JAMP-7810 manual in English and local chemical analysis

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Dear all,

We are making preparations for experiments on determination of the chemical
composition of semiconductor oxides, formed locally. The size of the
modified area should be about 1 micron, and we are not sure yet if it is
possible to make mentioned chemical analysis with Auger microprobe at all.

Another problem is that our newly came Auger spectroscope JAMP-7810 has no
English manual with it. And believe me, it is barely possible to read that
in Japanese!

We would be very grateful for your suggestions on both my questions:
- how to measure the best chemical bonding on the spot with 1x1 sq.
micrometer dimensions
- where to get the manual (or copy of it) from.
Remark: Mails to USA and Japanese representatives of JEOL gave no result!

Best regards.
Dmitri


__________________________________________
Dmitri V. Sokolov, Doctor Course student
Research Center for Interface Quantum Electronics,
Hokkaido University, North 13, West 8, Kitaku,
Sapporo 060, Hokkaido, Japan
Phone 81-11-706-7174 Fax 81-11-716-6004
http://www.geocities.com/SiliconValley/Campus/1314
AOL Instant Messenger FalconDot
ICQ 9418072
__________________________________________




From daemon Thu Feb 03 15:57:12 2000



From: bengi :      bengi-at-unimo.it
Date: Thu, 3 Feb 2000 14:54:20 +0100
Subject: Nitrogen determination

Contents Retrieved from Microscopy Listserver Archives
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Dear listers,
I have started a research project on ammonium -bearing mineral (zeolites),
and now I'm trying to quantify N using electron microprobe analysis. My
problems of dealing with N are:

a) The thickness of the coating (in the literateur = approximately 150 A¡).
Does anyone have information on the model of coater to making these films?
Does anyone have experience with preparation of such
samples?

b) Obtaining appropriate standards. (I have Si3N4, but it contains too much
N (=39.96%) towards my samples (5%). Does anyone known any good nitrogen
standards for such a sample ?

Any other suggestions about quantitative nitrogen analysis would be greatly
appreciated !!
Thanks for your time.

My best regards

Eugenia


Eugenia Marchi
Universitˆ degli Studi di Modena
Dip. Scienze della Terra
P.le S.Eufemia n¡.19
41100 Modena (Italy)
Tel. +39-059-417289
Fax. +39-059-417399
e-mail: bengi-at-unimo.it




From daemon Thu Feb 03 15:57:36 2000



From: Barr, Dennis B :      dennbarr-at-eastman.com
Date: Thu, 3 Feb 2000 09:30:07 -0500
Subject: RE: Silver membranes being discontinued

Contents Retrieved from Microscopy Listserver Archives
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It's unfortunate that this manufacturer is stopping production. Aren't
there other manufacturers?

Dennis B. Barr (dennbarr-at-eastman.com)
Physical Chemistry Research Laboratory
Physical & Analytical Chemistry Research Division
Eastman Chemical Company
Kingsport, TN 37662-5150

B-150B, R-132E, (423) 229-2188


} -----Original Message-----
} From: Garber, Charles A. [SMTP:cgarber-at-2spi.com]
} Sent: Wednesday, February 02, 2000 2:37 AM
} To: MICROSCOPY BB
} Subject: Silver membranes being discontinued
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
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} -----------------------------------------------------------------------.
}
}
} -- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --
}
} Hello,
}
} I apologize in advance if this posting offends anyone. However there are a
} good many people who use silver membranes in their work and to have the
} supply from the worlds only manufacturer (to my knowledge) come to a halt,
} has the potential of being highly disruptive at least to some programs:
} =================================================
} Osmonics has announced the closing of our Phoenix, AZ manufacturing
} facility
} effective 1 May 2000. Since our silver membranes are manufactured in this
} facility, Osmonics has decided to end production of this membrane because
} of
} declining sales and the very expensive costs associated with moving the
} mfg.
} .. plant to another location. All orders placed before 1 March, 2000 will
} be
} honored and filled.
} ==================================================
} We plan to make a "last buy" before the cut off date of March 1, 2000. I
} would advise anyone depending on these silver membranes for their work to
} take stock of their future requirements because after the cut off date,
} sales will be possible only from remaining stocks.
}
} For those who do run out, and are in a bind, we are prepared help them
} find
} alternative filtration media, of which there are indeed some alternatives
} for some applications.
}
} Chuck
}
} ============================================
}
} Charles A. Garber, Ph. D. Ph: 1-610-436-5400
} President 1-800-2424-SPI
} SPI SUPPLIES FAX: 1-610-436-5755
} PO BOX 656 e-mail:cgarber-at-2spi.com
} West Chester, PA 19381-0656 USA
} Cust.Service: spi2spi-at-2spi.com
}
} Look for us!
} ########################
} WWW: http://www.spi.cc
} ########################
} ============================================



From daemon Thu Feb 03 15:57:55 2000



From: Michael Bode :      mb-at-soft-imaging.com
Date: Thu, 3 Feb 2000 08:11:02 -0700
Subject: RE: Gray level

Contents Retrieved from Microscopy Listserver Archives
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Yes, I agree with John. A better question than "how many gray levels can
one see" is probably "how many gray levels can one see under what
circumstances". The answer is probably very different for a static image
and a dynamic image, with edges or without, in low light conditions or
bright light conditions.

The human eye (and brain) has had Millions of years to evolve and
develop some rather sophisticated algorithms to deal with different
conditions and the answer is probably also very complex. For example,
our vision is extremely good at detecting movement. On a completely
static image this tends to depress differences, while on a dynamic image
it tends to enhance differences (we automatically focus on the changes).


Maybe you can test that yourself:

take the same image on a computer at different bit depths (=levels of
gray). Then randomly put them on the screen (without watching the
change) and try to decide, which image it is. Then do the same but watch
the changes and try to decide how many gray levels you need before the
changeover becomes invisible. You could do this with a "noise" image, an
image that shows some object, and a smooth gray wedge. I for one would
be interested to hear about the results.

Michael

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
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===================================
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} ----------
} From:
"DrJohnRuss-at-aol.com"-at-sparc5.Microscopy.Com[SMTP:"DRJOHNRUSS-at-AOL.COM"-at-SPA
RC5.MICROSCOPY.COM]
} Sent: Wednesday, February 02, 2000 5:56:04 PM
} To: Microscopy-at-sparc5.Microscopy.Com; rgriffin-at-eng.uab.edu
} Subject: Re: Gray level
} Auto forwarded by a Rule
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In a message dated 2/2/00 4:00:26 PM,
rgriffin-at-eng.uab.edu-at-sparc5.Microscopy.Com writes:

} How many gray levels can the human eye distinguish? We've found
several
}
} references that disagree.
}
} If anyone knows of a reference - that would be best.

Most of the books on image processing say the same thing (probably all
copied
from each other and other non-prime sources), but I don't know of an
original
reference based on real research. You can find the same ideas in books
like
Frisby "Seeing: illusion, brain and mind", Oxford, 1980; Marr "Vision",

Freeman, 1982; and Rock "Perception", Freeman, 1984 which deal with the

human visual system not in the context of computer graphics. These are
probably closer to the original research.

The basic idea is that it takes about a 2-2.5% change in absolute
brightness
(i.e. a ratio of 1.025) to be just detectable, and only if it is a
fairly
sudden change spatially (or temporally for that matter). That is where
the
20-30 distinct brightness levels over the full range of brightness that
can
be seen at one time (i.e. without accommodations like varying pupil
size)
comes from. The requirement that an entire scene have about 100 levels
to
avoid banding is more controversial, based on the idea that the eye can
perform some accommodation as it traverses from one part of a scene to
another. This probably doesn't apply when (e.g.) looking through a
microscope, so the 20-30 number would be more applicable. It also
ignores
color, which complicates things further.



From daemon Thu Feb 03 15:57:56 2000



From: MICHAEL MOHN :      MMOHN-at-mail.monroe.cc.mi.us
Date: Thu, 03 Feb 2000 10:14:13 -0500
Subject: Re: LM Sony camera bayonet mount

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Most industrial cameras use C-mounts. C-mount to camera lens adapters are available from Edmund Industrial Optics (http://www.edmundoptics.com phone:800-363-1992) on page 165 of their 2000 catalog. They have mounts for several types of cameras, and list for $62 each. If your connecting the camera to a 'scope, Edmund sells a relay lens for $240 which mounts the camera to a 23mm eyepiece tube. For connecting to trinocular and CCD ports on a scope, Diagnostic Instruments sells relay lenses for many types of 'scopes (http://www.diaginc.com) for $299.


Michael Mohn
Assistant Professor of Materials Technology
Monroe County Community College
Phone: 734-384-4122 Fax: 734-242-9711
http://www.monroe.cc.mi.us/mmohn

} } } "Karl Hagglund-KW" by way of Nestor J. Zaluzec {hagglund.kw-at-pg.com} 02/02 6:06 PM } } }
We have a sony DXC-760 MD ccd camera that we have mounted to a standard camera
lens with a bayonet adapter. Unfortunately, we have borrowed the adapter and
have to return it. Is there a standard size (i.e. Nikon, Minolta, Canon) lens
adapter that works with this camera mount? We can't find a part number or
description for the adapter, and the book doesn't describe the size. We have
already ordered and returned one that didn't fit.

Thanks in advance.

Karl Hagglund
Proctor and Gamble
Food and Beverage Analytical Microbiology






From daemon Thu Feb 03 15:57:57 2000



From: rlvaughn-at-unmc.edu
Date: Thu, 3 Feb 2000 09:39:47 -0600
Subject: Re: Gray level

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Are you equating brightness levels with grey levels here?
I was told something like this years ago when we were buying our first
digital B&W camera, when I asked the vendor about the competitors thousand
plus grey levels vs his 256 grey levels and was told that the eye could not
distinguish greater levels. Now, four or five years later everyone is
pitching 10, 12 and 16? bit cameras, and yet the images from these cameras
do look better. Is it the bits and increased grey levels? I know that if
your doing image analysis at the pixel level that more bits are to your
advantage but what about just photgraphic quality?

Rick Vaughn



From daemon Thu Feb 03 15:58:08 2000



From: L. D. Marks :      ldm-at-apollo.numis.nwu.edu
Date: Thu, 3 Feb 2000 10:06:36 -0600 (CST )
Subject: Re: TEM:finding the parallel beam

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Sorry, but I think we are getting a little confused here.

1) The diffraction plane is (by definition) the objective lens
focal length below the sample - it is the focus of parallel
directions coming from the sample. The focal length (and
parameters such as Cs, Cc) change rapidly with the objective lens
current so an eucentric position makes life simple but is not
necessarily the best condition to use. (For HREM you generaly
want to have the objective lens stronger and reduce Cs.)

2) In general C2 does not change the focal length at all. You
can have some effect from coupling of the magnetic fields, but
all C2 is supposed to do is change the convergence angle.

3) The objective aperature is (approximately) in the back-focal
plane, given that it is finite and height adjustments are only
done coarsely (for a specific value of the objective current
only).

4) The selected area aperature is (approximately) in the same
plane as the object for the same reasons as above. Remember that
there are positional errors in SA diffraction due to Cs (see
Hirsch et al for instance).

5) You should focus Kikuchi-lines, not go for the smallest spot,
since these are real object in the diffraction pattern. What you
will see is then representative of you incident beam, sometimes
a Gaussian range of directions.

6) The simplest way to get more parallel illumination in general is
to reduce the condensor aperture size.

7) All bets are off if you have a FEG. Rather than having incoherent
illumination things like the Cs of the prefield (above the sample)
and postfield (below) add coherently making it much more complicated.
For instance, the illumination angle varies across the field of view.
While this is also there with LaB6, it is a weak effect (except for
certain classes of diffracted beams, e.g. those with a screw-axis). With
a FEG you can get a "spot" pattern many ways, and most of these will have
large aberrations (e.g. pincushion).

++++++++++++++++++++++++++++++++++++++++++++++++
Laurence Marks
Department of Materials Science and Engineering
Northwestern University
fax: (847) 491-7820
mailto:l-marks-at-nwu.edu
http://www.numis.nwu.edu
++++++++++++++++++++++++++++++++++++++++++++++++



From daemon Thu Feb 03 15:58:11 2000



From: Dr Eric Lachowski :      che136-at-abdn.ac.uk
Date: Thu, 3 Feb 2000 16:24:08 +0000 (GMT)
Subject: Re: Nitrogen determination

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Eugenia,
the microprobe is not the best tool for the analysis of
ammonia in zeolites because ammonia is very volatile in the
electron beam. If your zeolite is phase pure it would be
far better to dissolve it and do a classical chemical
analysis.

regards,
Eric
On Thu, 3 Feb 2000 14:54:20 +0100 bengi {bengi-at-unimo.it}
wrote:


} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
}
} Dear listers,
} I have started a research project on ammonium -bearing mineral (zeolites),
} and now I'm trying to quantify N using electron microprobe analysis. My
} problems of dealing with N are:
}
} a) The thickness of the coating (in the literateur = approximately 150 A°).
} Does anyone have information on the model of coater to making these films?
} Does anyone have experience with preparation of such
} samples?
}
} b) Obtaining appropriate standards. (I have Si3N4, but it contains too much
} N (=39.96%) towards my samples (5%). Does anyone known any good nitrogen
} standards for such a sample ?
}
} Any other suggestions about quantitative nitrogen analysis would be greatly
} appreciated !!
} Thanks for your time.
}
} My best regards
}
} Eugenia
}
}
} Eugenia Marchi
} Università degli Studi di Modena
} Dip. Scienze della Terra
} P.le S.Eufemia n°.19
} 41100 Modena (Italy)
} Tel. +39-059-417289
} Fax. +39-059-417399
} e-mail: bengi-at-unimo.it
}
}
}

----------------------
Dr Eric Lachowski
Department of Chemistry
University of Aberdeen
Meston Walk
Old Aberdeen AB24 3UE
Scotland
+44 (0)1224 272934 fax 272921
e.lachowski-at-abdn.ac.uk





From daemon Thu Feb 03 15:58:23 2000



From: L. Paul Bédard :      Paul_Bedard-at-uqac.uquebec.ca
Date: Thu, 3 Feb 2000 12:12:17 -0500
Subject: Need repairman for Hitachi SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,

I need somebody to repair a Hitachi SEM S-2700.
Please contact me individually not the list.
Thanks for help,
--
L.Paul Bédard, ing. Ph.D.
Resp. Lab. Géochimie | Lab. Manager
Sciences Appliquées ; Université du Québec à Chicoutimi Canada
Tél. : 418/545-5011 x 2276 | Alt. E-mail : pbedard-at-saglac.qc.ca
{} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {}
La période humaine, depuis la création d'Adam jusqu'à nos jours, n'est qu'une
moisissure dans l'histoire de notre planète
JCK Laflamme 1886



From daemon Thu Feb 03 15:58:37 2000



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Thu, 03 Feb 2000 11:22:46 -0600
Subject: Re: Gray level

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Let me offer my guess that the improved appearance of the images is be due
largely to the improved signal-to-noise ratio in the newer cameras.

Of course there is also something to be gained in the dynamic range by the
extra bits of resolution. They are much more forgiving and allow
substantial changes in contrast and brightness without sacrificing the
information contained in the data.

Warren S.

At 09:39 AM 2/3/2000 -0600, you wrote:

} Are you equating brightness levels with grey levels here?
} I was told something like this years ago when we were buying our first
} digital B&W camera, when I asked the vendor about the competitors thousand
} plus grey levels vs his 256 grey levels and was told that the eye could not
} distinguish greater levels. Now, four or five years later everyone is
} pitching 10, 12 and 16? bit cameras, and yet the images from these cameras
} do look better. Is it the bits and increased grey levels? I know that if
} your doing image analysis at the pixel level that more bits are to your
} advantage but what about just photgraphic quality?
}
} Rick Vaughn



From daemon Thu Feb 03 15:58:28 2000



From: Michael Plociniak :      plocinia-at-aecom.yu.edu
Date: Thu, 03 Feb 2000 12:44:23 -0500
Subject: New Gold Enhancement Reagents

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Colleagues,
Has anyone used gold enhancement reagents from Nanoprobes, Inc.
(Stonybrook, NY - USA) as an alternative to silver enhancement for
enlarging 1 nm immunogold probes?

Advertisements state that gold enhancement has lower background and is
compatible with osmium. Can anyone verify this from personal experience?

In addition to these advantages, I am hoping that milder pH conditions will
be less damaging to ultrastructure in cultured neurons (using a
preembedment protocol).

Thank you,
Michael



From daemon Thu Feb 03 15:58:41 2000



From: Sinkler, Wharton :      wharton.sinkler-at-anlw.anl.gov
Date: Thu, 03 Feb 2000 12:13:10 -0700
Subject: Re: TEM:finding the parallel beam

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Rado,

} The diffraction plane is always the crossover plane not the theoretical back
} focal plane of the objective. Its z-position changes with the change of the
} C2 excitement.

Your statement above is not wrong, but isn't the standard TEM viewpoint (or
terminology). Yes, the plane at which the spot is sharpest (the in focus
plane for the demagnified filament image) is the crossover plane. However
to generally call this the "diffraction plane" is confusing. For example in
the case of CBED, the crossover plane has moved (up or down, depending on
whether C2 was initially under or overfocused) to the specimen plane. This
doesn't make the specimen plane the "diffraction plane". Rather, the
"diffraction plane" is now considered the in-focus plane for the C2
aperture. This is at the objective lens back-focal plane - the plane at
which points on the specimen are magnified to infinity and where the
position variable corresponds to the illumination angle. In collecting an
SADP you can correct for slightly non-parallel illumination by correcting
diffraction focus slightly off of the true back focal plane, but this should
be only slight.

What was proposed in the original mail was a technique of determining how
parallel the incident beam is, and of improving things by tweaking C2. If
the beam isn't very parallel, you'll see the diffraction spots shift when
you move the SA aperture. This is because the incident beam inclination
depends on the position on the sample.

I would think the "tweak" to C2 for obtaining more parallel illumination
would always in the direction of greater beam spread (unless I am
misunderstanding something?).

Regards,
Wharton
++++++++++++++++++++++++++++++++++++++++++++++++++
Wharton Sinkler
Argonne National Laboratory West
P. O. Box 2528
Idaho Falls, ID 83403



} Rado
}
} ---------------------------------------------------------------------
} Radostin Danev
} Laboratory of Ultrastructure Research
} National Institute for Physiological Sciences
} Myodaiji-cho, Okazaki 444-8585, JAPAN
} e-mail: rado-at-nips.ac.jp
} ---------------------------------------------------------------------
} ----- Original Message -----
} } From: Jonathan Barnard {Jonathan.Barnard-at-Angstrom.UU.SE}
} To: {microscopy-at-sparc5.microscopy.com}
} Sent: Wednesday, February 02, 2000 7:20 PM
} Subject: TEM:finding the parallel beam
}
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } I got a tip for finding the condenser lens settings required for parallel
} } illumination from Angus Kirkland (Cambridge).
} }
} } Make sure you are in microprobe mode and set up for SAED and the specimen
} } is out of the way. Spread the beam, put in the SA Aperture, go to
} } diffraction and then sweep the SA aperture (SAA) from side to side and
} } minimise the deflection of the diffraction spot by tweaking the
} } illumination control (C2 or second condenser lens). A little bit of
} thought
} } will convince you that anything other than a parallel beam will give you a
} } side to side motion (tilt) when the SAA is swept.
} }
} } I have found it useful to keep a table of C2 condenser lens current
} } settings for each spot size (C1 excitation) in microprobe mode. Setting
} the
} } C2 lens for parallel illumination and adjusting the diffraction focus to
} } get the sharpest spot will then give you near perfect SAD conditions.
} }
} } Anyone contemplating electron holography for example, will have to start
} } from the parallel illumination to get the best spatial coherence for their
} } holograms.
} }
} } ********************************************************
} } Dr Jonathan Barnard
} }
} } Analytical Materials Physics
} } The Angstrom Laboratory, Uppsala University
} } P O Box 534, SE-751 21 Uppsala, Sweden
} } Phone: +46-(0)18-4716838 Fax: +46-(0)18-500131
} } http://www.angstrom.uu.se/analytical/home.html
} }
} } ********************************************************
} }
} }
}



From daemon Thu Feb 03 15:59:01 2000



From: Carl Henderson :      chender-at-umich.edu
Date: Thu, 3 Feb 2000 15:11:42 -0500
Subject: Re: Nitrogen determination

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Eugenia,

You can avoid your concern with C coating by coating your standards
and unknowns at the same time. You should use the minimum coating
thickness--just enough to prevent beam charging.

However, as stated below by Dr. Lachowski, ammonia volatility would
be a big problem, especially since you will need help from high beam
intensities and/or long counting times to get enough N counts to give
decent precision. The expected intensity measured with a 10kV beam
from a zeolite nominally of analcime composition (NaAlSi2O6.H2O) with
5 wt% N would be about 2.5% that of the intensity from Si3N4. High N
intensity is difficult to achieve even with the best spectrometer and
crystal designs. And to add insult to injury, the addition of the C
coating absorbs even more of the N x-rays from the sample. The mass
absorption coefficient for N K-alpha emission with a C absorber is
huge (greater than 23,000).

Plus, you should check for possible N peak shape differences between
Si3N4 (or other standards) and your ammonium-bearing zeolite.

All in all, it is a very difficult analytical problem.

Good luck!

Carl

At 4:24 PM +0000 2/3/00, Dr Eric Lachowski wrote:
} Eugenia,
} the microprobe is not the best tool for the analysis of
} ammonia in zeolites because ammonia is very volatile in the
} electron beam. If your zeolite is phase pure it would be
} far better to dissolve it and do a classical chemical
} analysis.
}
} regards,
} Eric
} On Thu, 3 Feb 2000 14:54:20 +0100 bengi {bengi-at-unimo.it}
} wrote:
}
} }
} } Dear listers,
} } I have started a research project on ammonium -bearing mineral (zeolites),
} } and now I'm trying to quantify N using electron microprobe analysis. My
} } problems of dealing with N are:
} }
} } a) The thickness of the coating (in the literateur =
} approximately 150 A°).
} } Does anyone have information on the model of coater to making these films?
} } Does anyone have experience with preparation of such
} } samples?
} }
} } b) Obtaining appropriate standards. (I have Si3N4, but it contains too much
} } N (=39.96%) towards my samples (5%). Does anyone known any good nitrogen
} } standards for such a sample ?
} }
} } Any other suggestions about quantitative nitrogen analysis would be greatly
} } appreciated !!
} } Thanks for your time.
} }
} } My best regards
} }
} } Eugenia
} }
} }
} } Eugenia Marchi
} } Università degli Studi di Modena
} } Dip. Scienze della Terra
} } P.le S.Eufemia n°.19
} } 41100 Modena (Italy)
} } Tel. +39-059-417289
} } Fax. +39-059-417399
} } e-mail: bengi-at-unimo.it
} }
} }
} }
}
} ----------------------
} Dr Eric Lachowski
} Department of Chemistry
} University of Aberdeen
} Meston Walk
} Old Aberdeen AB24 3UE
} Scotland
} +44 (0)1224 272934 fax 272921
} e.lachowski-at-abdn.ac.uk

======================================
Carl Henderson
Electron Microbeam Analysis Laboratory
University of Michigan
2501 C.C. Little Bldg.
Ann Arbor, MI 48109-1063 USA
(734) 936-1550 FAX (734) 763-4690
======================================



From daemon Thu Feb 03 17:53:40 2000



From: Paul.Nolan-at-Alcan.Com
Date: Thu, 3 Feb 2000 16:05:19 -0600
Subject: Technoorg-Linda Ion Beam Thinner

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


To: {Microscopy-at-MSA.Microscopy.Com}


Does anyone out there have experience with the Technoorg-Linda IV3H ion beam
thinner.
We have their instruction manual but it has been a long time since we had a
training session and are having some problem with the set up.
Does anyone have a revised instruction manual or perhaps some tips on the
alignment procedure of the guns. (please please please please).

Thanks
Paul Nolan

P.S..
It is cold in Canada..everyday...all year. hehe




From daemon Thu Feb 03 17:53:43 2000



From: Thomas, Larry :      Larry.Thomas-at-pnl.gov
Date: Thu, 03 Feb 2000 14:10:01 -0800
Subject: RE: TEM:finding the parallel beam

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Laurie,

Now I'm really confused. I used to think I understood this somewhat.

1. In the JEOL 2010F FEG (the "all bets are off" case in your item#7),
changing the illumination spread by changing C2 profoundly moves the
"diffraction plane" relative to the objective aperture. [There are actually two
objective apertures at different heights in this microscope, but that's beside
the point]. By diffraction plane, I mean the plane below the objective lens
where the diffraction spots are in sharp focus. In other words (assuming fixed
OL and condenser minilens excitations), in this microscope there is only one C2
value that allows the diffraction spot pattern and OL aperture to be in focus
simultaneously. If you focus on the OL aperture (with the intermediate lens),
the spot pattern can be focused at only this one C2 setting. At any other C2
setting, you can focus the diffraction pattern with the intermediate lens
("diffraction focus") but the OL aperture will then be defocused. Changing the
condenser minilens excitation changes the diffraction plane (relative to the OL
aperture) to a different C2 setting. This is actually quite a useful feature of
the microscope, e.g., for getting very intense focused diffraction patterns, but
a different story.

2. The reason for choosing to focus the diffraction spots rather than
the K-lines is --to put it simply-- that's where the intensity is. In amplitude
contrast imaging (conventional brightfield and darkfield imaging), the intensity
contribution to the image is limited by the OL aperture. It's really important
to have the defining aperture and diffraction spot pattern in focus
simultaneously. In addition, if I aim to measure diffraction spot patterns
there is little incentive to focus the K-lines instead of the spots.

3. Textbooks such as Hirsch et al. often discuss the positional error
in SA diffraction due to Cs. What readers often miss is the consequence of the
fact that the error depends strongly on the diffraction angle. If measurements
are confined to the low-index (low-angle) reflections, the selecting area can be
reduced (to 200 nm or so at 200 kV) without big positional errors. Maybe that's
an example of differences in experimental and theoretical viewpoints.

4. Reducing the condensor aperture size will give less illumination,
but it won't give parallel illumination. It will change the size of the
diffraction spots, but not their focus.

5. I'm not quite sure why the illumination spread has such a strong
effect on the apparent position of the diffraction plane relative to the OL
aperture, but suspect it arises from the action of the strong
condensor-objective in this microscope. I would also question that it has much
to do with coherent/incoherent beams in a FEG or with prefield/postfield Cs
effects.

Larry


Larry Thomas
Pacific Northwest National Laboratory
MSIN P8-16
P.O. Box 999
Richland, WA 99352
Phone: (509)372-0793 Fax: (509)376-6308
Email: mailto: Larry.Thomas-at-pnl.gov



----------
From: L. D. Marks
Reply To: L-marks-at-nwu.edu
Sent: Thursday, February 3, 2000 8:06 AM
To: Radostin Danev
Cc: MSA listserver
Subject: Re: TEM:finding the parallel beam

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America
To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
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-----------------------------------------------------------------------.


Sorry, but I think we are getting a little confused here.

1) The diffraction plane is (by definition) the objective lens
focal length below the sample - it is the focus of parallel
directions coming from the sample. The focal length (and
parameters such as Cs, Cc) change rapidly with the objective lens
current so an eucentric position makes life simple but is not
necessarily the best condition to use. (For HREM you generaly
want to have the objective lens stronger and reduce Cs.)

2) In general C2 does not change the focal length at all. You
can have some effect from coupling of the magnetic fields, but
all C2 is supposed to do is change the convergence angle.

3) The objective aperature is (approximately) in the back-focal
plane, given that it is finite and height adjustments are only
done coarsely (for a specific value of the objective current
only).

4) The selected area aperature is (approximately) in the same
plane as the object for the same reasons as above. Remember that
there are positional errors in SA diffraction due to Cs (see
Hirsch et al for instance).

5) You should focus Kikuchi-lines, not go for the smallest spot,
since these are real object in the diffraction pattern. What you
will see is then representative of you incident beam, sometimes
a Gaussian range of directions.

6) The simplest way to get more parallel illumination in general is
to reduce the condensor aperture size.

7) All bets are off if you have a FEG. Rather than having incoherent
illumination things like the Cs of the prefield (above the sample)
and postfield (below) add coherently making it much more complicated.
For instance, the illumination angle varies across the field of view.
While this is also there with LaB6, it is a weak effect (except for
certain classes of diffracted beams, e.g. those with a screw-axis).
With
a FEG you can get a "spot" pattern many ways, and most of these will
have
large aberrations (e.g. pincushion).

++++++++++++++++++++++++++++++++++++++++++++++++
Laurence Marks
Department of Materials Science and Engineering
Northwestern University
fax: (847) 491-7820
mailto:l-marks-at-nwu.edu
http://www.numis.nwu.edu
++++++++++++++++++++++++++++++++++++++++++++++++




From daemon Thu Feb 03 17:54:04 2000



From: David Henriks :      Henriks-at-CompuServe.COM
Date: Thu, 3 Feb 2000 17:57:35 -0500
Subject: Technoorg-Linda Ion Beam Thinner

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Paul:

South Bay Technology does have quite a bit of experience with the TL IV3
Ion Mill and we are happy to offer our assistance. We market the IV3
system throughout the world for Technoorg-Linda and offer technical support
as well. I will send you the most up to date IV3 manual that we have
produced and will include by separate e-mail the section on the gun
alignment.

I will follow up with you to be sure that you have all of the information
you need.

Best regards-

David
Writing at 3:50:09 PM on 02/03/2000

***************************************************************************
************************

David Henriks
Vice President TEL: 800-728-2233
(toll free in the USA)
South Bay Technology, Inc. +1-949-492-2600
1120 Via Callejon FAX: +1-949-492-1499
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

***************************************************************************
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} } } } } Please visit us at http://www.southbaytech.com { { { { {

Manufacturers of precision sample preparation equipment and supplies for
metallography, crystallography and electron microscopy.

Message text written by
INTERNET:"Paul.Nolan-at-Alcan.Com"-at-sparc5.Microscopy.Com
}
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Does anyone out there have experience with the Technoorg-Linda IV3H ion
beam
thinner.
We have their instruction manual but it has been a long time since we had
a
training session and are having some problem with the set up.
Does anyone have a revised instruction manual or perhaps some tips on the
alignment procedure of the guns. (please please please please).

Thanks
Paul Nolan

P.S..
It is cold in Canada..everyday...all year. hehe
{



From daemon Thu Feb 03 17:54:01 2000



From: L. D. Marks :      ldm-at-risc4.numis.nwu.edu
Date: Thu, 3 Feb 2000 17:08:04 -0600 (CST)
Subject: RE: TEM:finding the parallel beam

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On Thu, 3 Feb 2000, Thomas, Larry wrote:

} Laurie,
}
} Now I'm really confused. I used to think I understood this somewhat.
}
} 1. In the JEOL 2010F FEG (the "all bets are off" case in your item#7),
} changing the illumination spread by changing C2 profoundly moves the
} "diffraction plane" relative to the objective aperture. [There are actually two
} objective apertures at different heights in this microscope, but that's beside
} the point]. By diffraction plane, I mean the plane below the objective lens
} where the diffraction spots are in sharp focus. In other words (assuming fixed
} OL and condenser minilens excitations), in this microscope there is only one C2
} value that allows the diffraction spot pattern and OL aperture to be in focus
} simultaneously. If you focus on the OL aperture (with the intermediate lens),
} the spot pattern can be focused at only this one C2 setting. At any other C2
} setting, you can focus the diffraction pattern with the intermediate lens
} ("diffraction focus") but the OL aperture will then be defocused. Changing the
} condenser minilens excitation changes the diffraction plane (relative to the OL
} aperture) to a different C2 setting. This is actually quite a useful feature of
} the microscope, e.g., for getting very intense focused diffraction patterns, but
} a different story.
}
OK, the bets off case. In a FEG you have a somewhat coherent
range of incident directions. The "diffraction pattern" is therefore
something which is effected by the coherent aberrations of the microscope,
unlike the case with incoherent illumination. Consider the case with
focussed illumination, when the diffraction pattern shows an image of
the condensor aperture. In a FEG As a you can "focus" this (coherent)
image to a small spot by going out of focus in a true sense for the
diffraction pattern. This gives you a spot-like pattern, but you will also
see severe distortions at higher angles. With incoherent illumination you
cannot do this and going out of focus (in the diffraction pattern) will
give in general a blurred image of the condensor aperture - life is
simple.
What you need to do is focus "real diffraction" features, e.g.
Kikuchi lines, then not play with the diffraction focus at all. As you
change C2 the size of the spots will grow or shrink, this is fine.

} 2. The reason for choosing to focus the diffraction spots rather than
} the K-lines is --to put it simply-- that's where the intensity is. In amplitude
} contrast imaging (conventional brightfield and darkfield imaging), the intensity
} contribution to the image is limited by the OL aperture. It's really important
} to have the defining aperture and diffraction spot pattern in focus
} simultaneously. In addition, if I aim to measure diffraction spot patterns
} there is little incentive to focus the K-lines instead of the spots.
}
Yes, you can always get a "spot" pattern with a FEG, and it may
be prettier. However, beware the distortions which make doing
measurements from it very dangerous.

} 3. Textbooks such as Hirsch et al. often discuss the positional error
} in SA diffraction due to Cs. What readers often miss is the consequence of the
} fact that the error depends strongly on the diffraction angle. If measurements
} are confined to the low-index (low-angle) reflections, the selecting area can be
} reduced (to 200 nm or so at 200 kV) without big positional errors. Maybe that's
} an example of differences in experimental and theoretical viewpoints.
}
Hirsch et al is just a good reference to read, about this as well
as many other things!

} 4. Reducing the condensor aperture size will give less illumination,
} but it won't give parallel illumination. It will change the size of the
} diffraction spots, but not their focus.
}
It makes it closer to parallel. Of course, with really parallel
illumination there is no intensity!

} 5. I'm not quite sure why the illumination spread has such a strong
} effect on the apparent position of the diffraction plane relative to the OL
} aperture, but suspect it arises from the action of the strong
} condensor-objective in this microscope. I would also question that it has much
} to do with coherent/incoherent beams in a FEG or with prefield/postfield Cs
} effects.
}
Sorry, I don't think that is correct. I would suggested that
people look at the distortions with a standard sample and see how bad they
can be.

} Larry
}
}
} Larry Thomas
} Pacific Northwest National Laboratory
} MSIN P8-16
} P.O. Box 999
} Richland, WA 99352
} Phone: (509)372-0793 Fax: (509)376-6308
} Email: mailto: Larry.Thomas-at-pnl.gov
}
}
}
} ----------
} From: L. D. Marks
} Reply To: L-marks-at-nwu.edu
} Sent: Thursday, February 3, 2000 8:06 AM
} To: Radostin Danev
} Cc: MSA listserver
} Subject: Re: TEM:finding the parallel beam
}
} ------------------------------------------------------------------------
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} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Sorry, but I think we are getting a little confused here.
}
} 1) The diffraction plane is (by definition) the objective lens
} focal length below the sample - it is the focus of parallel
} directions coming from the sample. The focal length (and
} parameters such as Cs, Cc) change rapidly with the objective lens
} current so an eucentric position makes life simple but is not
} necessarily the best condition to use. (For HREM you generaly
} want to have the objective lens stronger and reduce Cs.)
}
} 2) In general C2 does not change the focal length at all. You
} can have some effect from coupling of the magnetic fields, but
} all C2 is supposed to do is change the convergence angle.
}
} 3) The objective aperature is (approximately) in the back-focal
} plane, given that it is finite and height adjustments are only
} done coarsely (for a specific value of the objective current
} only).
}
} 4) The selected area aperature is (approximately) in the same
} plane as the object for the same reasons as above. Remember that
} there are positional errors in SA diffraction due to Cs (see
} Hirsch et al for instance).
}
} 5) You should focus Kikuchi-lines, not go for the smallest spot,
} since these are real object in the diffraction pattern. What you
} will see is then representative of you incident beam, sometimes
} a Gaussian range of directions.
}
} 6) The simplest way to get more parallel illumination in general is
} to reduce the condensor aperture size.
}
} 7) All bets are off if you have a FEG. Rather than having incoherent
} illumination things like the Cs of the prefield (above the sample)
} and postfield (below) add coherently making it much more complicated.
} For instance, the illumination angle varies across the field of view.
} While this is also there with LaB6, it is a weak effect (except for
} certain classes of diffracted beams, e.g. those with a screw-axis).
} With
} a FEG you can get a "spot" pattern many ways, and most of these will
} have
} large aberrations (e.g. pincushion).
}
} ++++++++++++++++++++++++++++++++++++++++++++++++
} Laurence Marks
} Department of Materials Science and Engineering
} Northwestern University
} fax: (847) 491-7820
} mailto:l-marks-at-nwu.edu
} http://www.numis.nwu.edu
} ++++++++++++++++++++++++++++++++++++++++++++++++
}
}
}

++++++++++++++++++++++++++++++++++++++++++++++++
Laurence Marks
Department of Materials Science and Engineering
Northwestern University
fax: (847) 491-7820
mailto:L-marks-at-nwu.edu
http://www.numis.nwu.edu
++++++++++++++++++++++++++++++++++++++++++++++++




From daemon Thu Feb 03 20:37:06 2000



From: Tobias Baskin :      BaskinT-at-missouri.edu
Date: Fri, 4 Feb 2000 12:30:27 +1100
Subject: Re: LSCM Need help with non-fluorescing resin

Contents Retrieved from Microscopy Listserver Archives
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Greetings,
Butyl methyl methacrylate is in our hands non fluorescent
totally. You can use it for TEM, although it is not as good as epoxies.
Hope this helps.
Tobias
}
}
} I am working on fluorescent imaging of thin sections cut from samples
} embedded in TEM embedding resin, and am having a significant problem with
} resin fluorescence. Does anyone know of an embedding resin that doesn't
} autofluoresce?
}
} Matt Plantinga
} Amway Corporation
} matt_plantinga-at-amway.com



From daemon Thu Feb 03 20:36:55 2000



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Date: Thu, 03 Feb 2000 19:34:55
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From daemon Thu Feb 03 17:54:05 2000



From: ctschristopher :      ctschristopher-at-samiot.uct.ac.za (by way of Carol
Date: Thu, 3 Feb 2000 17:56:46 -0800
Subject: a question about 2-hydroxyhexane-dial, AKA hydroxyadipaldehyde

Contents Retrieved from Microscopy Listserver Archives
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LIst,

Does anyone know of a current source for this chemical? It's an ingredient
of an embedding medium made up by our group. We have a supply on hand but
have to reserve it if it's no longer commercially available.


} } We are very excited about the possibilities of HACH as a solution for a
} } problem
} } that has troubled us for a couple of years with our PU grafts. We have,
} } unfortunately, one serious problem in that we cannot find a source for HA.
} } We have tried Sigma Aldrich and they informed us that they withdrew
} } 2-Hydroxyhexane-dial from their range in 1990. We have tried a web search
} } and are contacting our local suppliers, so far without success. Do you have
} } any ideas where we can find it? We are very anxious to try HACH on our PU
} } samples and will gratefully appreciate any additional help that you can give
} } us.

} } Thanks again

} } Phil




From daemon Fri Feb 04 21:48:26 2000



From: Dr Malcolm Roberts :      malc-at-rock.ru.ac.za
Date: Fri, 04 Feb 2000 09:04:34 +0200
Subject: Silence

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Have I been cut off, or is nobody talking anymore?

--
Dr MP Roberts Phone: [+27](0)46 603 8313
Dept of Geology Fax: [+27](0)46 622 9715
Rhodes University e-mail: malc-at-rock.ru.ac.za
6140 Grahamstown
SOUTH AFRICA




From daemon Fri Feb 04 21:48:27 2000



From: Alexander Mironov Jr. :      amironov-at-cmns.mnegri.it
Date: Fri, 4 Feb 2000 08:51:22 +0100 (MET)
Subject: Re: New Gold Enhancement Reagents

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Dear Michael,

I have tried GoldEnhance for preembedding protocol to label intracellular
structures. It is marvelous. Buy it and use it - you will not be
unsatisfied. I have no any interest in Nanoprobes, I just like these dense
gold particles.
Practically all they claim is true:
- light insensitive
- no self-nucleation
- do not react with buffer ions
- is not dissolved by uranil and osmium (I have treat for 1 h)

Sincerely,
Dr. Alexander A. Mironov Jr., MD, PhD
Unit of Morphology
Dept. of Cell Biology and Oncology
Consorzio Mario Negri Sud
Via Nazionale, S.Maria Imbaro (Ch)
66030 Italy

Tel. 0039-0872-570-332
Fax 0039-0872-570-412
E-mail: amironov-at-cmns.mnegri.it


On Thu, 3 Feb 2000, Michael Plociniak wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear Colleagues,
} Has anyone used gold enhancement reagents from Nanoprobes, Inc.
} (Stonybrook, NY - USA) as an alternative to silver enhancement for
} enlarging 1 nm immunogold probes?
}
} Advertisements state that gold enhancement has lower background and is
} compatible with osmium. Can anyone verify this from personal experience?
}
} In addition to these advantages, I am hoping that milder pH conditions will
} be less damaging to ultrastructure in cultured neurons (using a
} preembedment protocol).
}
} Thank you,
} Michael
}
}



From daemon Fri Feb 04 21:48:31 2000



From: Ken Tiekotter :      tiekotte-at-up.edu
Date: Fri, 4 Feb 2000 00:13:57 -0800 (PST)
Subject: Re: Help:A comparison of optics

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Dear Steve and interested parties:

The comparison between Zeiss and Olympus '100x' objective lenses is
impossible unless one knows the type of lens be compared.

Catagories used to determine the quality of such a lens include:
aberration correction (spherical and chromatic), correction of flatness of
field, numerical aperture, the presence of an iris diaphram, whether the
system is based on a 160mm tube length or infinity correction, and if the
lens is used for brightfield or phase contrast for instance.

(PLAN APO, PH3, 100x, iris diaphram, 1.32 numerical aperture? 160mm tube
length)

Resolution is still basically related to half the wavelength utilized.
Hey, let's look at the advantages of oblique illumination for contrast
enhancement and a differerent formula for resolution!!

Is your client looking at a system or an indivdual lens? Do they have a
Zeiss or an Olympus system? One does not mixed and match objectives from
different systems merely for the convenience of pricing.

An additional consideration is the type and correction of the condenser
lens. Check the numerical aperture of not only the objective, but the
condenser lens as well.

More questions than answers...Let me know if I can further complicate the
answers!

Cheers!
Ken

------------
Ken Tiekotter
Dept. of Biology
The University of Portland
5000 N Willamette Blvd.
Portland, OR 97303


On Thu, 3 Feb 2000, Steve Niemela wrote:

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} To: {Microscopy-at-MSA.Microscopy.Com}
} Subject: A comparison of optics
} Date: Thu, 3 Feb 2000 10:57:19 -0600
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}
} A client of ours wonders about a comparison between Zeiss and Olympus
} optics. The Zeiss 100X lense cost $10,000, and the Olympus about $4,000:
} does that mean the Olympus is inferior?  That's what our client's
} colleagues imply. What measure of lense function is relevant? Does Olympus
} (or Zeiss) have a specification, like 'resolves particles to 0.1 micron'
} or something like that?  Is there a measure of distortion? Best
} regards, Steve NiemelaVayTek, Inc.305 West Lowe AvenueFairfield, IA
} 52556email: sniemela-at-vaytek.com Phone: 515.472.2227Fax: 515.472.8131web:
} www.vaytek.comgeneral email: vaytek-at-vaytek.com
}
}
}
}



From daemon Fri Feb 04 21:48:33 2000



From: Janko Otto :      otto-at-quantifoil.com
Date: Fri, 04 Feb 2000 09:47:44 +0100
Subject: Re: JAMP-7810 manual in English and local chemical analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Dmitri,

} We are making preparations for experiments on determination of the chemical
} composition of semiconductor oxides, formed locally. The size of the
} modified area should be about 1 micron, and we are not sure yet if it is
} possible to make mentioned chemical analysis with Auger microprobe at all.

I can recommend you some former colleagues of mine performing auger
spectroscopy and many other semiconductor surface analytics.
Please contact Prof. Wolfgang Richter: richter-at-pinet.uni-jena.de.
The URL is: http://www.physik.uni-jena.de/~layer/

Regards,
Kay Pfennighaus


--
_____________________________________________________________

Janko Otto/Kay Pfennighaus email otto-at-quantifoil.com
Quantifoil Micro Tools GmbH Tel +49 (0) 3641 - 206 470
Winzerlaer Strasse 10 Fax +49 (0) 3641 - 206 471
D-07745 Jena Web http://www.quantifoil.com
Germany
_____________________________________________________________



From daemon Fri Feb 04 21:48:35 2000



From: Tony Bruton :      bruton-at-EMU.UNP.AC.ZA
Date: Fri, 04 Feb 2000 11:38:49 +0200
Subject: Re: Silence

Contents Retrieved from Microscopy Listserver Archives
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W'ere all waiting to hear news of MSSA Conference 2000 { :-)

Tony

} } } Dr Malcolm Roberts {malc-at-rock.ru.ac.za} 02/04/00 09:04AM } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Have I been cut off, or is nobody talking anymore?

--
Dr MP Roberts Phone: [+27](0)46 603 8313
Dept of Geology Fax: [+27](0)46 622 9715
Rhodes University e-mail: malc-at-rock.ru.ac.za
6140 Grahamstown
SOUTH AFRICA






From daemon Fri Feb 04 21:48:38 2000



From: A.Walker :      Alan.Walker-at-sheffield.ac.uk
Date: Fri, 4 Feb 2000 10:10:23 +0000
Subject: Re: Technoorg-Linda Ion Beam Thinner

Contents Retrieved from Microscopy Listserver Archives
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Hi Paul,

} Does anyone out there have experience with the Technoorg-Linda IV3H ion beam
} thinner.
} We have their instruction manual but it has been a long time since we had a
} training session and are having some problem with the set up.
} Does anyone have a revised instruction manual or perhaps some tips on the
} alignment procedure of the guns. (please please please please).

We have the IV3 here in Sheffield - I'll try and help you if I can.
You need to be able to actually see the beams (cover your head and
the viewing glass with a black sheet if you cannot darken the room).
Get one gun to fire through the center of the sample holder (tilt it
at right angles to the beam) and to hit the opposite gun where it's
beam would exit. Do the same for the other gun. Now keep this gun
orientation, load the specimen and tilt the holder to the desired milling angle.

Hope this helps,

Alan Walker

*********************************************
Alan Walker
Dept of Electronic and Electrical Engineering
University of Sheffield
Mappin Street
Sheffield S1 3JD
United Kingdom

Tel:+44-(0)114-2225365
Fax:+44-(0)114-2726391
alan.walker-at-shef.ac.uk
http://www.shef.ac.uk/uni/academic/D-H/eee/
*********************************************



From daemon Fri Feb 04 21:48:59 2000



From: Donald Lovett :      lovett-at-tcnj.edu
Date: Fri, 4 Feb 2000 09:17:35 -0500 (EST)
Subject: Is KEVEX 8000 worth installing?

Contents Retrieved from Microscopy Listserver Archives
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A Kevex 8000 (with huge 8 or 10" disk drives), attached to an old Amray
1000, has been donated to us. All components were fully functional when
they were crated up a year ago. (They are sitting in our basement until we
move into our new building this June).

A colleague in Physics (I'm a biologist) wishes to use the system to
quantify elemental composition (beryllium, gallium, etc) in semi-conductor
ceramics. We already have (up and running) an Hitachi HS-510 (SEM) and an
Hitachi H-7100 (STEM).

1) Is this (Kevex 8000) an appropriate instrument for her to do the
analyses?

2) Which would be wiser: Trying to get the Amray installed and running
or getting an adapter to use the Kevex in one of the Hitachi's? (Space is
a consideration, so I'd prefer not to have to install (and keep in running
condition) another scope.)

3) The Kevex is pretty old. Are we wasting money trying to get or keep
running something that is this old? I realize that we may need to replace
the detector, since it has been at room temp for about a year. Is there
anything else we should automatically consider replacing or upgrading as
part of the installation?

4) She also has been sending her samples out to do x-ray analysis of the
material to determine it's crystalline structure. The place she sends it
to has a special x-ray diffraction instrument. Could the x-ray
diffraction on the H-7100 be used to get equal results, or does this other
instrument have capabilities that the TEM doesn't.

Thanks for any advice.

Don
______________________________________________________________________
Donald L. Lovett e-mail: lovett-at-tcnj.edu
Assoc. Professor, Dept. of Biology voice: (609) 771-2876
P.O. Box 7718 fax: (609) 637-5118
The College of New Jersey
Ewing, NJ 08628-0718








From daemon Fri Feb 04 21:49:36 2000



From: Barbara Foster :      mme-at-map.com
Date: Fri, 04 Feb 2000 10:20:50 -0500
Subject: Re: Help:A comparison of optics

Contents Retrieved from Microscopy Listserver Archives
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Steve,

The cost of an objective depends on a number of issues, not the least of
which are the corrections for chromatic and spherical aberration as well as
the size of the numerical aperture. I'd need more information to really
compare. "Optimizing Light Microscopy" has a detailed discussion of all of
this which might be helpful. Ordering information is available on our
website.

The best test is to try each system with your applications. Since much of
Vaytek's work centers on deconvolution of fluorescence images, fluorescent
beads of varying sizes would be a good test object for you and high
throughput and crisp imaging would probably be two key benchmarks. Since
fluorescence detects objects beyond the resolution limits, the normal
resolution tests are meaningless. By the way, the numerical aperture of
the objective is the major deciding factor on a microscope's ability to
resolve fine detail (as well as providing good edge information).

If you'd like to write or call me directly, I may be able to give you
further guidance.

Best regards,
Barbara Foster
Consortium President
Microscopy/Microscopy Education ...Educating microscopists for greater
productivity.

125 Paridon Street Suite 102 Springfield, MA 01118
PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com
Visit our web site {http://www.MME-Microscopy.com/education}


Caveat: MME does have a commercial interest in the sale of "Optimizing
Light MIcroscopy:"

At 04:04 PM 2/3/00 -0600, Steve Niemela wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Fri Feb 04 21:49:47 2000



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Fri, 04 Feb 2000 09:52:32 -0600
Subject: Re: Is KEVEX 8000 worth installing?

Contents Retrieved from Microscopy Listserver Archives
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If your Kevex has a Quantum thin window detector you should be able to see
about half of a Boron peak, but there is no commercially available detector
(that I know of) that reliably sees all of the beryllium peak. And if you
have a standard window, you will only be able to see sodium and above.

I suppose the detector may still be functional even after storage. I recall
that it is not a trivial thing to switch detectors between scope models.
The adapters can be quite different and can get pricey. But I have not
priced one in over 10 years.

I suppose with rigorous use of standards, you might be able to analyze Be
by difference, but it would be a trick.

I think the Kevex 8000 may be worth installing if you are primarily
interested in x-ray analysis. We used a Kevex Delta for many years and
found it quite adequate. I might be able to talk you through some of the
technical issues.

However, if you are interested in digital imaging or x-ray mapping, the
improvements in the new equipment is fantastic compared to the old systems.
We run an IXRF Systems EDS on a JEOL 840A (having replaced our Kevex) and a
Link ISIS on a Hitachi 2460N. I could hardly imagine giving up their
capabilities and going back to the Delta or an 8000. Disk storage, the
operating environment, digital imaging can hardly be compared between the
old and the new.

FWIW,
Warren

At 09:17 AM 2/4/2000 -0500, Donald L. Lovett wrote:
} A Kevex 8000 (with huge 8 or 10" disk drives), attached to an old Amray
} 1000, has been donated to us. All components were fully functional when
} they were crated up a year ago. (They are sitting in our basement until we
} move into our new building this June).
}
} A colleague in Physics (I'm a biologist) wishes to use the system to
} quantify elemental composition (beryllium, gallium, etc) in semi-conductor
} ceramics. We already have (up and running) an Hitachi HS-510 (SEM) and an
} Hitachi H-7100 (STEM).
}
} 1) Is this (Kevex 8000) an appropriate instrument for her to do the
} analyses?
}
} 2) Which would be wiser: Trying to get the Amray installed and running
} or getting an adapter to use the Kevex in one of the Hitachi's? (Space is
} a consideration, so I'd prefer not to have to install (and keep in running
} condition) another scope.)
}
} 3) The Kevex is pretty old. Are we wasting money trying to get or keep
} running something that is this old? I realize that we may need to replace
} the detector, since it has been at room temp for about a year. Is there
} anything else we should automatically consider replacing or upgrading as
} part of the installation?
}
} 4) She also has been sending her samples out to do x-ray analysis of the
} material to determine it's crystalline structure. The place she sends it
} to has a special x-ray diffraction instrument. Could the x-ray
} diffraction on the H-7100 be used to get equal results, or does this other
} instrument have capabilities that the TEM doesn't.
}
} Thanks for any advice.
}
} Don



From daemon Fri Feb 04 21:49:48 2000



From: Jim McGee :      mcgee-at-geol.sc.edu
Date: Fri, 04 Feb 2000 10:55:56 -0500
Subject: Re: Nitrogen determination

Contents Retrieved from Microscopy Listserver Archives
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Carl et al.,

I responded to Eugenia's query on another listserver (MAS microprobe listserver),
but since it is also discussed here, I will add a couple things.

I did some work on ammonium-bearing minerals (with ~ 3 - 5 wt%N), using BN and later
synthetic buddingtonite (ammonium feldspar) as the N standard (see Microbeam
Analysis 1994, New Orleans meeting). I avoided using any epoxy for the sample prep
as a precaution against N artifacts from the mounting medium. The synthetic
buddingtonite can be made ala Voncken et al., 1988 (Phys. Chem of Minerals, v. 15,
p. 323).

We did "normal" carbon coating, using brass color as a guide to obtain 150-200A
coating (standards and unknowns). I used a synthetic crystal, 2d 60A, and ran at
10kV, 25 nA probe current. I agree that a light C coat, done at same time/conditions
as the standards is important. I don't recall a substantial problem with mobility
at thnese operating conditions, although the N intensity yields were low and
detection limits were on the order of 0.5wt% N. The minerals are feldpar and
perhaps the ammonium ion stayed put better than it woud in zeolites. I agree
though, it is a difficult analytical problem. But that makes it interesting.

Jim
--
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
James J. McGee (email: jmcgee-at-sc.edu)
Department of Geological Sciences
University of South Carolina
Columbia, SC 29208

Tel: 803-777-6300 Fax: 803-777-6610




Carl Henderson wrote:

} ------------------------------------------------------------------------
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} -----------------------------------------------------------------------.
}
} Eugenia,
}
} You can avoid your concern with C coating by coating your standards
} and unknowns at the same time. You should use the minimum coating
} thickness--just enough to prevent beam charging.
}
} However, as stated below by Dr. Lachowski, ammonia volatility would
} be a big problem, especially since you will need help from high beam
} intensities and/or long counting times to get enough N counts to give
} decent precision. The expected intensity measured with a 10kV beam
} from a zeolite nominally of analcime composition (NaAlSi2O6.H2O) with
} 5 wt% N would be about 2.5% that of the intensity from Si3N4. High N
} intensity is difficult to achieve even with the best spectrometer and
} crystal designs. And to add insult to injury, the addition of the C
} coating absorbs even more of the N x-rays from the sample. The mass
} absorption coefficient for N K-alpha emission with a C absorber is
} huge (greater than 23,000).
}
} Plus, you should check for possible N peak shape differences between
} Si3N4 (or other standards) and your ammonium-bearing zeolite.
}
} All in all, it is a very difficult analytical problem.
}
} Good luck!
}
} Carl
}
} At 4:24 PM +0000 2/3/00, Dr Eric Lachowski wrote:
} } Eugenia,
} } the microprobe is not the best tool for the analysis of
} } ammonia in zeolites because ammonia is very volatile in the
} } electron beam. If your zeolite is phase pure it would be
} } far better to dissolve it and do a classical chemical
} } analysis.
} }
} } regards,
} } Eric
} } On Thu, 3 Feb 2000 14:54:20 +0100 bengi {bengi-at-unimo.it}
} } wrote:
} }
} } }
} } } Dear listers,
} } } I have started a research project on ammonium -bearing mineral (zeolites),
} } } and now I'm trying to quantify N using electron microprobe analysis. My
} } } problems of dealing with N are:
} } }
} } } a) The thickness of the coating (in the literateur =
} } approximately 150 A°).
} } } Does anyone have information on the model of coater to making these films?
} } } Does anyone have experience with preparation of such
} } } samples?
} } }
} } } b) Obtaining appropriate standards. (I have Si3N4, but it contains too much
} } } N (=39.96%) towards my samples (5%). Does anyone known any good nitrogen
} } } standards for such a sample ?
} } }
} } } Any other suggestions about quantitative nitrogen analysis would be greatly
} } } appreciated !!
} } } Thanks for your time.
} } }
} } } My best regards
} } }
} } } Eugenia
} } }
} } }
} } } Eugenia Marchi
} } } Università degli Studi di Modena
} } } Dip. Scienze della Terra
} } } P.le S.Eufemia n°.19
} } } 41100 Modena (Italy)
} } } Tel. +39-059-417289
} } } Fax. +39-059-417399
} } } e-mail: bengi-at-unimo.it
} } }
} } }
} } }
} }
} } ----------------------
} } Dr Eric Lachowski
} } Department of Chemistry
} } University of Aberdeen
} } Meston Walk
} } Old Aberdeen AB24 3UE
} } Scotland
} } +44 (0)1224 272934 fax 272921
} } e.lachowski-at-abdn.ac.uk
}
} ======================================
} Carl Henderson
} Electron Microbeam Analysis Laboratory
} University of Michigan
} 2501 C.C. Little Bldg.
} Ann Arbor, MI 48109-1063 USA
} (734) 936-1550 FAX (734) 763-4690
} ======================================



From daemon Fri Feb 04 21:49:50 2000



From: Augusto_A_Morrone-at-notes.seagate.com
Date: Fri, 4 Feb 2000 09:58:13 -0600
Subject: JEOL 2010 question/C2, diffraction and OA

Contents Retrieved from Microscopy Listserver Archives
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Since the discussion on CM12 alignment shifted to several other problems, and
eventually got some JEOL 2010 users involved (Larry Thomas), I thought of
throwing in this alignment question regarding the OA in a 2010 with a LaB6 gun:

To keep the camera length at a consistent value when I am not using an internal
standard, I record SA diffraction patterns with C2 fully defocused cw (this is
by conventional jargon, although actually using the Brightness knob I am
changing C3). This is supposed to make the illumination as parallel as possible
and is easily reproducible. Then I focus the direct beam with the intermediate
lens (diffraction focus). CBD and NBD are another problem.

The problem is to align the Objective Aperture under this condition. If the OA
is centered around the direct beam in diffraction, when switching to image mode
and increasing the illumination (Brightness) for imaging conditions, the
aperture will be apparently out of center. This gives the impression that the
voltage center changes between a fully defocused C2 and a moderately focused
(still cw from crossover, converging the illumination on the sample) condenser.
That shift is obvious in DIFF mode, the direct beam can be seen to shift as C2
is turned ccw from the fully cw position.

As was noted in the on-going discussion, the crossover planes move along the
optic axis as the strength of C2 changes. The helicoidal path of the beam is
straight down the optic axis of the lenses only piecewise, and at the level of
the OA plane it stays centered only for a limited range of C2 settings--or
convergence angles. In my case, the convergence changes considerably when I
record images digitally in a 1kx1k CCD camera, which screams for brightness.

My two ways around this are to (1) recenter the OA in image mode, or (2) in DIFF
mode, but keeping C2 at the setting I will use in MAG mode. Both methods are a
pain, mostly when doing BF/DF work, and probably incorrect.

Is there an alignment procedure for the 2010 that will prevent this " OA shift "
from DIFF to MAG modes?

Augusto Morrone
Seagate Technology
NRW-115
7801 Computer Ave S.
(612) 844-5838
Fax: (612) 844-7301




From daemon Fri Feb 04 21:49:54 2000



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Fri, 04 Feb 2000 08:42:59 -0800
Subject: Re: Need repairman for Hitachi SEM

Contents Retrieved from Microscopy Listserver Archives
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Dear Paul,
Why don't you use the Hitachi service from NSC? They are the best and the
cheapest.
At 12:12 PM 2/3/00 -0500, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Fri Feb 04 21:49:59 2000



From: Lesley Weston :      lesley-at-interchange.ubc.ca
Date: Fri, 4 Feb 2000 10:12:49 -0800 (PST)
Subject: Aqueous mounting medium

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Can anyone recommend an aqueous mounting medium that polymerises (or
whatever) to form a permanent hard mount, and that does not
autofluoresce? Thanks.

Lesley Weston.





From daemon Fri Feb 04 21:50:02 2000



From: Douglas Keene :      DRK-at-SHCC.ORG
Date: Fri, 04 Feb 2000 10:36:37 -0800 (Pacific Standard Time)
Subject: New Gold Enhancement Reagents

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Michael,

We had been using silver enhancement kits from Amersham and
Nanoprobe. Of these, we found the Nanoprobe kit to be very
difficult to work with given it's high viscosity. We also
use a pre-embed protocol, and found that both silver
enhancement kits caused collagen fibrils to denature
(unravel). However, during our protocol the tissue is
incubated for fairly long duration in the enhancement
medium (15 minutes on ice, then warmed to 25 C in a water
bath for an additional 5 minutes, all in the dark).

Our more recent experience is with the Nanoprobe gold
enhancement kit. It has HUGE advantages over the silver
system.

First, you can Osmicate the tissue. Second, the viscosity
of the components is comparatively very low, easing the use
of the product. It is not so sensitive to light, and it
does not cause denaturation of collagen fibrils. We do see
some variability in particulate size, but this is probably
a problem associated with diffusion of the media through
the tissue. We have found no disadvantages of the Gold
enhancement v.s. Silver and now use the Gold method
exclusively.

I hope this helps,

Doug

On Thu, 03 Feb 2000 12:44:23 -0500 Michael Plociniak
{plocinia-at-aecom.yu.edu} wrote:

}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy
} Society of America To Subscribe/Unsubscribe -- Send Email
} to ListServer-at-MSA.Microscopy.Com On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear Colleagues,
} Has anyone used gold enhancement reagents from Nanoprobes,
} Inc. (Stonybrook, NY - USA) as an alternative to silver
} enhancement for enlarging 1 nm immunogold probes?
}
} Advertisements state that gold enhancement has lower
} background and is compatible with osmium. Can anyone
} verify this from personal experience?
}
} In addition to these advantages, I am hoping that milder pH
} conditions will be less damaging to ultrastructure in
} cultured neurons (using a preembedment protocol).
}
} Thank you,
} Michael

----------------------
Douglas R. Keene
Associate Investigator
Shriners Hospital Research Facilities
3101 S.W. Sam Jackson Park Road
Portland, Oregon 97201
phone: 503-221-3434
FAX: 503-412-6894 (9-5 PST)
e-mail: DRK-at-shcc.org






From daemon Fri Feb 04 21:50:14 2000



From: Dan Freidus :      freidus-at-wwnet.com
Date: Fri, 4 Feb 2000 14:38:50 -0500
Subject: Re: Help:A comparison of optics

Contents Retrieved from Microscopy Listserver Archives
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-----Original Message-----
} From: Ken Tiekotter {tiekotte-at-up.edu}
..One does not mixed and match objectives from different systems merely for
the convenience of pricing....
----------------------------------------------------------------------------
---------------------------------------------------------------------------

Of course, I've heard this sort of statement many times before. But has
anyone ever done any comparisons of mixed systems to see if there are
certain brands that are particularly incompatible or particularly
compatible?

I ask this both as a general question and because I use a Wild M7 for which
I need a pair of high eyepoint oculars. I don't need the adjusting collar
built into current models and haven't been able to find an older pair used.
But before I buy a Nikon/Olympus/ etc. pair I'd love to know how different
the complete system will be from what I've got now.

I know many people who have used third party photo eyepieces, in just the
situation where you'd think quality would be of the utmost concern. Dealers
are often not the best source of info since they tend to represent one brand
and, even when they are completely honest, rarely have experienc ewith a
wide range of brands (especially mixed up.

Dan Freidus
freidus-at-wwnet.com

P.S. Of course, if anyone has a pair of Wild 10x high eyepoint oculars for
sale, that would also solve my problem (I'd also consider other
magnifications). (I'm also looking for accessory objective lenses and
various other accessories. Please contact me off-list if you've got anything
for sale or trade.)






From daemon Fri Feb 04 21:51:06 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 04 Feb 2000 15:02:28 -0600
Subject: Re: Help:A comparison of optics

Contents Retrieved from Microscopy Listserver Archives
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At 09:20 AM 2/4/00 , you wrote:
} } A client of ours wonders about a comparison between Zeiss and Olympus
} } optics. The Zeiss 100X lense cost $10,000, and the Olympus about $4,000:
} } does that mean the Olympus is inferior?  That's what our client's
} } colleagues imply. What measure of lense function is relevant? Does Olympus
} } (or Zeiss) have a specification, like 'resolves particles to 0.1 micron'
} } or something like that?  Is there a measure of distortion? Best
} } regards, Steve NiemelaVayTek, Inc.305 West Lowe AvenueFairfield, IA
} } 52556email: sniemela-at-vaytek.com Phone: 515.472.2227Fax: 515.472.8131web:
} } www.vaytek.comgeneral email: vaytek-at-vaytek.com
} }

The NA is very important in achieving high resolution. The other factor is
the NA of the condenser. An Abbe condenser is easily out gunned by
the aplanatic condenser (NA=1.4). Olympus makes two PlanAPO 100X oil
objectives. One has an NA of 1.3 while the other is 1.4. The 1.4 lens
costs today new about $6000. The lower NA lens is about $4500. So this
is probably the one you are comparing. I had a Zeiss PlanAPO and recall
that it had an NA of 1.3. It was terrible. I replaced it with the Olympus 1.4
and got major improvements. Of the Zeiss objectives, their 63X PlanAPO
is probably the best one they have made. The rest are so so. Some are
easily beaten by Olympus flourites. Zeiss is more expensive because it
is Zeiss, not necessarily because it is better. The cost of manufacturing
in Germany is very high compared to Japan and other places that
Olympus uses. The emerging problem I see is that Olympus is moving much
of its scope production to China. I have purchased all of the Olympus
items I need and that were made in Japan before being stuck with Chinese
Olympus. It may reach a point where Zeiss is a better product despite the
higher cost.

BTW, the 1.4NA lens at $6000 reflects the recent 10% across the board
price increase by Olympus.

gary g.



From daemon Fri Feb 04 21:50:38 2000



From: Sinkler, Wharton :      wharton.sinkler-at-anlw.anl.gov
Date: Fri, 04 Feb 2000 14:14:10 -0700
Subject: Re: TEM:finding the parallel beam

Contents Retrieved from Microscopy Listserver Archives
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Listers,

There are important differences between older and more recent TEMs, which go
to the heart of this discussion.

On older dedicated HREM's (LaB6) imaging is done with the beam at or close
to crossover on the specimen, and beam convergence is determined by the user
using the C2 aperture. On newer TEM's such as the JEOL 2010 (FEG or not),
you need to set C2 so crossover is pretty far from the specimen plane in
order to get decent coherence for imaging. Whenever this is the case, the
C2 aperture isn't incoherently filled, and some of the older theory won't
apply (regardless of whether the machine is or isn't a FEG).

For example, the standard method of determining convergence in an image is
by switching to diffraction under the same conditions used to image, and
measuring the disc diameter. (see e.g. O'Keefe and Sanders, Acta Cryst A31,
p.307). This assumes the beam being near or at crossover on the specimen
aperture. It won't work if the beam is far from crossover on the specimen,
because now the incident angle depends on position on the specimen. The
disc diameters in diffraction only indicate what the total range of incident
angles is over the entire illuminated area. But the area visible in the
HREM image is much smaller than this, and over this latter area the range of
illuminating angles is smaller. This is just another way of saying that the
C2 aperture isn't incoherently filled. If it were, would be impossible to
form a shadow image of the C2 aperture at the specimen plane, as one does by
spreading the beam.

Think of the illumination on the specimen as a convolution of the source
image with the circular C2 aperture: When the beam is at crossover, the
aperture part is approximating a delta function - we see the source in the
image. When the beam is spread very far, the illumination is a nice shadow
image of the C2 aperture, with a little blurriness at the edge because of
convolution with a source which is not quite point-like.

In the latter case, the angular spread locally is given by the source size
(demagnification) in the diffraction pattern. This can be imaged by
switching to diffraction and focusing with the intermediate lens to obtain
sharp source images.

This doesn't depend on whether the machine is FEG or not, though the source
will be very much smaller if it is. I have posted an example in which a
tungsten filament is imaged sharply by defocusing with the intermediate lens
with the illumination just slightly defocused. This should be a square
pattern, so note that the optics introduce significant distortions in this
extreme case.

http://www.numis.nwu.edu/internet/Staff/wharton/sources.jpg


++++++++++++++++++++++++++++++++++++++++++++++++++
Wharton Sinkler
Argonne National Laboratory West
P. O. Box 2528
Idaho Falls, ID 83403

}
}
} On Thu, 3 Feb 2000, Thomas, Larry wrote:
}
} } Laurie,
} }
} } Now I'm really confused. I used to think I understood this somewhat.
} }
} } 1. In the JEOL 2010F FEG (the "all bets are off" case in your item#7),
} } changing the illumination spread by changing C2 profoundly moves the
} } "diffraction plane" relative to the objective aperture. [There are actually
} } two
} } objective apertures at different heights in this microscope, but that's
} } beside
} } the point]. By diffraction plane, I mean the plane below the objective lens
} } where the diffraction spots are in sharp focus. In other words (assuming
} } fixed
} } OL and condenser minilens excitations), in this microscope there is only one
} } C2
} } value that allows the diffraction spot pattern and OL aperture to be in focus
} } simultaneously. If you focus on the OL aperture (with the intermediate
} } lens),
} } the spot pattern can be focused at only this one C2 setting. At any other C2
} } setting, you can focus the diffraction pattern with the intermediate lens
} } ("diffraction focus") but the OL aperture will then be defocused. Changing
} } the
} } condenser minilens excitation changes the diffraction plane (relative to the
} } OL
} } aperture) to a different C2 setting. This is actually quite a useful feature
} } of
} } the microscope, e.g., for getting very intense focused diffraction patterns,
} } but
} } a different story.
} }
} OK, the bets off case. In a FEG you have a somewhat coherent
} range of incident directions. The "diffraction pattern" is therefore
} something which is effected by the coherent aberrations of the microscope,
} unlike the case with incoherent illumination. Consider the case with
} focussed illumination, when the diffraction pattern shows an image of
} the condensor aperture. In a FEG As a you can "focus" this (coherent)
} image to a small spot by going out of focus in a true sense for the
} diffraction pattern. This gives you a spot-like pattern, but you will also
} see severe distortions at higher angles. With incoherent illumination you
} cannot do this and going out of focus (in the diffraction pattern) will
} give in general a blurred image of the condensor aperture - life is
} simple.
} What you need to do is focus "real diffraction" features, e.g.
} Kikuchi lines, then not play with the diffraction focus at all. As you
} change C2 the size of the spots will grow or shrink, this is fine.
}
} } 2. The reason for choosing to focus the diffraction spots rather than
} } the K-lines is --to put it simply-- that's where the intensity is. In
} } amplitude
} } contrast imaging (conventional brightfield and darkfield imaging), the
} } intensity
} } contribution to the image is limited by the OL aperture. It's really
} } important
} } to have the defining aperture and diffraction spot pattern in focus
} } simultaneously. In addition, if I aim to measure diffraction spot patterns
} } there is little incentive to focus the K-lines instead of the spots.
} }
} Yes, you can always get a "spot" pattern with a FEG, and it may
} be prettier. However, beware the distortions which make doing
} measurements from it very dangerous.
}
} } 3. Textbooks such as Hirsch et al. often discuss the positional error
} } in SA diffraction due to Cs. What readers often miss is the consequence of
} } the
} } fact that the error depends strongly on the diffraction angle. If
} } measurements
} } are confined to the low-index (low-angle) reflections, the selecting area can
} } be
} } reduced (to 200 nm or so at 200 kV) without big positional errors. Maybe
} } that's
} } an example of differences in experimental and theoretical viewpoints.
} }
} Hirsch et al is just a good reference to read, about this as well
} as many other things!
}
} } 4. Reducing the condensor aperture size will give less illumination,
} } but it won't give parallel illumination. It will change the size of the
} } diffraction spots, but not their focus.
} }
} It makes it closer to parallel. Of course, with really parallel
} illumination there is no intensity!
}
} } 5. I'm not quite sure why the illumination spread has such a strong
} } effect on the apparent position of the diffraction plane relative to the OL
} } aperture, but suspect it arises from the action of the strong
} } condensor-objective in this microscope. I would also question that it has
} } much
} } to do with coherent/incoherent beams in a FEG or with prefield/postfield Cs
} } effects.
} }
} Sorry, I don't think that is correct. I would suggested that
} people look at the distortions with a standard sample and see how bad they
} can be.
}
} } Larry
} }
} }
} } Larry Thomas
} } Pacific Northwest National Laboratory
} } MSIN P8-16
} } P.O. Box 999
} } Richland, WA 99352
} } Phone: (509)372-0793 Fax: (509)376-6308
} } Email: mailto: Larry.Thomas-at-pnl.gov
} }
} }
} }
} } ----------
} } From: L. D. Marks
} } Reply To: L-marks-at-nwu.edu
} } Sent: Thursday, February 3, 2000 8:06 AM
} } To: Radostin Danev
} } Cc: MSA listserver
} } Subject: Re: TEM:finding the parallel beam
} }
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Sorry, but I think we are getting a little confused here.
} }
} } 1) The diffraction plane is (by definition) the objective lens
} } focal length below the sample - it is the focus of parallel
} } directions coming from the sample. The focal length (and
} } parameters such as Cs, Cc) change rapidly with the objective lens
} } current so an eucentric position makes life simple but is not
} } necessarily the best condition to use. (For HREM you generaly
} } want to have the objective lens stronger and reduce Cs.)
} }
} } 2) In general C2 does not change the focal length at all. You
} } can have some effect from coupling of the magnetic fields, but
} } all C2 is supposed to do is change the convergence angle.
} }
} } 3) The objective aperature is (approximately) in the back-focal
} } plane, given that it is finite and height adjustments are only
} } done coarsely (for a specific value of the objective current
} } only).
} }
} } 4) The selected area aperature is (approximately) in the same
} } plane as the object for the same reasons as above. Remember that
} } there are positional errors in SA diffraction due to Cs (see
} } Hirsch et al for instance).
} }
} } 5) You should focus Kikuchi-lines, not go for the smallest spot,
} } since these are real object in the diffraction pattern. What you
} } will see is then representative of you incident beam, sometimes
} } a Gaussian range of directions.
} }
} } 6) The simplest way to get more parallel illumination in general is
} } to reduce the condensor aperture size.
} }
} } 7) All bets are off if you have a FEG. Rather than having incoherent
} } illumination things like the Cs of the prefield (above the sample)
} } and postfield (below) add coherently making it much more complicated.
} } For instance, the illumination angle varies across the field of view.
} } While this is also there with LaB6, it is a weak effect (except for
} } certain classes of diffracted beams, e.g. those with a screw-axis).
} } With
} } a FEG you can get a "spot" pattern many ways, and most of these will
} } have
} } large aberrations (e.g. pincushion).
} }
} } ++++++++++++++++++++++++++++++++++++++++++++++++
} } Laurence Marks
} } Department of Materials Science and Engineering
} } Northwestern University
} } fax: (847) 491-7820
} } mailto:l-marks-at-nwu.edu
} } http://www.numis.nwu.edu
} } ++++++++++++++++++++++++++++++++++++++++++++++++
} }
} }
} }
}
} ++++++++++++++++++++++++++++++++++++++++++++++++
} Laurence Marks
} Department of Materials Science and Engineering
} Northwestern University
} fax: (847) 491-7820
} mailto:L-marks-at-nwu.edu
} http://www.numis.nwu.edu
} ++++++++++++++++++++++++++++++++++++++++++++++++
}
}



From daemon Fri Feb 04 21:51:05 2000



From: Jaci Lett :      jmlett-at-cid.wustl.edu
Date: Fri, 4 Feb 2000 16:01:45 -0600
Subject: TEM: B-glucuronidase localization

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We are looking to localize B-glucuronidase in the mouse cochlea. We'd like
to stain for the enzyme and still be able to decalcify the cochleas and
process them into Epon-Araldite for LM and TEM without losing the reaction
product. Does anyone have any suggestions for staining or localization
protocols for this enzyme? I'm not certain whether the tissue will be taken
all the way to TEM, but I do know we'll be looking at LM (1 to 4 microns).
I found a method by Hayashi and Hayashi ("Electron Microscopic Cytochemistry
and Immunocytochemistry in Biomedicine", CRC Press, 1993) using hexazonium
pararosanilin (red in LM and electron dense for EM) on 40 micron non-frozen
sections (and then processed for EM) that sounds appropriate, but I'm
concerned that it may not work en bloc (the cochlea is a twisty, windy
beasty).

I posted this request a few months ago and received no assistance (just
individuals also interested in similar protocols and a company promoting
their antibody).

Thanks so much for any direction I can get,

Jaclynn M. Lett, Research Assistant jmlett-at-cid.wustl.edu

Fay and Carl Simons Center for Biology of Hearing and Deafness
Central Institute for the Deaf
818 S. Euclid Ave.
St. Louis, MO 63110

voice: 314-977-0257 fax: 314-977-0030



From daemon Fri Feb 04 21:51:15 2000



From: Jonathan Barnard :      Jonathan.Barnard-at-Angstrom.UU.SE
Date: Sat, 05 Feb 2000 00:45:21 +0100
Subject: TEM: Jeol 2010 question

Contents Retrieved from Microscopy Listserver Archives
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So the problem as I have understood it is:

===} changing C3 (brightness) leads to a shift of the bright field (BF)
beam in the back focal plane (BFP) causing the objective aperture to appear
off centre after converging the beam?

If you have aligned the gun properly then I suspect that the (shift) wobble
alignment has not been done properly.
When this button is pressed the microscope goes over to diffraction and you
should see a pair of discs or spots (which represent the two extremes in
tilt when the beam is shifted to its own extremes). The beam tilts are
adjusted to bring the two discs/spots into coincidence. This is done for
both the X and Y shift coils separately.
The X wobble button shifts the beam back and forth (X shift coils), but in
the back focal plane the spot or disc should remain stationary. This is
what you are trying to achieve with this alignment procedure. Afterwards
this should give you a beam that expands about the same point in the back
focal plane. You should find that the objective aperture remains centred in
both diff and image modes after doing this alignment.

As for the crossovers, yes you are right, they do move up and down when
changing the brightness. The back focal plane remains stationary (by
definition as L.Marks said). Anything other than a parallel beam should
give you a disc in the BFP (which represents the angular distribution of
rays incident on the objective lens).

What did you mean by a voltage centre? My interpretation of this is that
the source high tension is wobbled and any shift in the (previously
focussed) image is minimised by tuning the beam tilts. This alignment would
be used for energy filtered TEM (EFTEM) when doing elemental mapping on a
Gatan Imaging Filter (GIF) for example.



********************************************************
Dr Jonathan Barnard

Analytical Materials Physics
The Angstrom Laboratory, Uppsala University
P O Box 534, SE-751 21 Uppsala, Sweden
Phone: +46-(0)18-4716838 Fax: +46-(0)18-500131
http://www.angstrom.uu.se/analytical/home.html

********************************************************



From daemon Fri Feb 04 21:51:14 2000



From: Stephen A. Shaffer :      sshaffer-at-microdataware.com
Date: Fri, 04 Feb 2000 15:47:58 -0800
Subject: LM Meeting Announcement, San Francisco Microscopical Society

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The San Francisco Microscopical Society Announces its next regular
meeting:

Wednesday, February 9, 2000

Randall Museum
199 Museum Way
San Francisco, CA 94114-1429
(415) 554-9600

7:00 pm

Topic: Introduction to Phase Contrast Microscopy
by Robert D. Griffin, President, SFMS
And: Annual Business Meeting and Election of Officers

Further information, including an important Letter to the Members from
President Griffin, is available at http://www.microdataware.com/sfms .

The San Francisco Microscopical Society (SFMS) is a small, informal
group of light microscopists who meet on a monthly basis to discuss
topics of common interest. All are invited and welcome, regardless of
knowledge level or professional field.



From daemon Fri Feb 04 21:51:19 2000



From: James Pawley :      jbpawley-at-facstaff.wisc.edu
Date: Sat, 5 Feb 2000 14:39:24 +1100
Subject: 5th Live-cell Course at UBC: June 19-29

Contents Retrieved from Microscopy Listserver Archives
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Dear Steve,
Because of the nomenclature and the way manufacturers determine their
specifications, it is really difficult to compare optics on paper. When I
find myself in these types of discussions, I find it more useful to compare
optics of similar list price rather than specifications because generally
speaking, in our industry, the more things cost, the more care is taken in
their manufacture. Olympus has a new 100X objective with an NA of 1.65 that
sells for $10k. That would be the optic I would compare to the $10k Zeiss.

Shane Collins
Scientific Instruments

-----Original Message-----
} From: Steve Niemela [mailto:sniemela-at-vaytek.com]
Sent: Thursday, February 03, 2000 2:05 PM
To: microscopy-at-sparc5.microscopy.com


To: {Microscopy-at-MSA.Microscopy.Com}


SECOND ANNOUNCEMENT: UBC LIVE-CELL COURSE: Please register by March!!

Hello all,

The faculty for the 2000 UBC 3D Live-cell Microscopy Course is now set and
we welcome several new faces, including Stephan Hell, Andreas Kriete and
Steve Potter. The whole list is below.

o Stephen Adams University of California-SD
o Ping Chin Cheng SUNY, Buffalo
o Elaine Humphrey Univ. of British Columbia
o Stephan Hell Max Planck, Goettingen
o Ted InouŽ Universal Imaging, PA
o Iain Johnson Molecular Probes, OR
o Larry Keenan Cell Robotics, NM
o Ernst Keller Carl Zeiss, Thornwood, NY
o Andreas Kriete University of Geissen
o Felix Margadant University of Sydney
o Tim Murphy University of British Columbia
o Jim Pawley University of Wisconsin-Madison
o Steve Potter California Institute of Tech
o Jason Swedlow University of Aberdeen
o Michael Weis Agriculture Canada


Basic info is below but you can get the entire brochure, including links to
faculty home pages, at

http://www.cs.ubc.ca/spider/ladic/course/brochure.htm

Cheers,

Jim P.

Fifth Annual INTERNATIONAL 10-Day Short Course on

3D Microscopy of Living Cells
June 19 - 29, 2000



Fourth, Post-course Workshop on

3D Image Processing,
July 1 - 3, 2000



Organized by Prof. James Pawley,
(University of Wisconsin-Madison)
(SEE SABBATICAL ADDRESS AT END OF MESSAGE)


in association with Dr. Elaine Humphrey
UBC BioSciences Microscopy Facility:
University of British Columbia
Vancouver, BC, Canada



DATES

Applications must be received by March 1, 2000
Deposit due April 15, 2000
Registration 5:00 - 7:00 PM Sunday, June 18, 2000
First Lecture 7:30 PM Sunday, June 18, 2000
Live-cell Course ends, noon Thursday, June 29, 2000


APPLICATIONS DUE BY MARCH 1, 2000


More information at :
http://www.cs.ubc.ca/spider/ladic/course/bulletin.html

or

REGARDING COURSE ORGANIZATION:


Prof. James B. Pawley, (on Sabbatical)
Room LG 10, Madsen Building, F-09,
University of Sydney, NSW, 2006
Australia

Ph. 61-2-9351-7548/2351
FAX 61-2-9351-7682
JBPAWLEY-at-FACSTAFF.WISC.EDU

REGARDING APPLICATIONS

Dr. Elaine Humphrey,
Biosciences EM Facility
Biosciences Building
Univ. of British Columbia
6270 University Blvd.
Vancouver, BC, V6T-1Z4




THE PURPOSE OF THE COURSE

Modern methods of 3D light microscopy promise a revolutionary improvement
in our ability to view living cells. To help convert this promise to
reality for a wider selection of biological scientists, an intensive
eleven-day residential course concentrating on all aspects of 3D Microscopy
of Living Cells will be held at the University of British Columbia, in June
of 2000. The course includes 4 days on 2D techniques, 6 days of 3D
techniques and a summary presentation. It covers basic microscopy to the
highest level confocal microscopy. (A half-day Pre-course is offered for
any who may need to brush up on basic optics!).

Topics include:
o Quantitative 2D light microscopy
o How to keep your cells alive
o 3D imaging in confocal microscopy
o Widefield/deconvolution techniques
o Two-photon excitation microscopy
o Fluorescent and backscattered light signals
o Dye design, characteristics and use
o Pixelation: The Nyquist Criterion
o Lasers and laser tweezers
o Objectives and aberrations
o Scanning-systems: AODs and mirrors
o Optimal pinhole size/photon efficiency
o Detectors: operation and performance
o Video-rate confocal imaging
o Measuring ion concentrations
o Display and measurement of 3D data

Morning lecture/demonstrations lead to hands-on laboratory exercises each
afternoon that will utilize most of the commercial instruments currently
available for 3D microscopic imaging. Students will work in groups of 3 or
4 throughout the discussion and laboratory sessions, and may complete a
live-cell 3D study on their own specimens. In the first four years, over
100 students from 22 countries have attended. Last year, 11 separate, 3D
microscopical workstations were available for student use under the
supervision of a 17-member international faculty. We expect to have even
more workstations in 2000. Including manufacturers representatives, the
teacher/ student ratio will be almost 2:1.

INTERNATIONAL FACULTY

o Stephen Adams University of California-SD
o Ping Chin Cheng SUNY, Buffalo
o Elaine Humphrey Univ. of British Columbia
o Stephan Hell Max Planck, Goettingen
o Ted InouŽ Universal Imaging, PA
o Iain Johnson Molecular Probes, OR
o Larry Keenan Cell Robotics, NM
o Ernst Keller Carl Zeiss, Thornwood, NY
o Andreas Kriete University of Geissen
o Felix Margadant University of Sydney
o Tim Murphy University of British Columbia
o Jim Pawley University of Wisconsin-Madison
o Steve Potter California Institute of Tech
o Jason Swedlow University of Aberdeen
o Michael Weis Agriculture Canada

TUITION

Course tuition is $2,150 US and includes lunches. On receipt of 50%
deposit, students will receive preliminary group assignments and the
textbook, Handbook of Biological Confocal Microscopy, (Plenum, 1995). The
tuition fee includes the textbook, a binder of handouts, and tickets for
the Opening Reception, the Manufacturer's Reception and the Beach Party.
Accommodations and meals other than lunch are not included in the tuition
fee. The Pre-course is $100 US.

APPLICATIONS

Applicants will complete a questionnaire to assess knowledge level and
field of interest. Enrollment is limited to about 24 participants.
Selection will be made on the basis of background and perceived need.
Those without previous LM experience will be provided with basic texts on
request to read before the course begins and are encouraged to take the
Pre-course on the afternoon of June 18.


Application forms, and other course information from this and past years,
can be downloaded from the WWW site at:

http://www.cs.ubc.ca/spider/ladic/course/bulletin.html

or obtained from:

Dr. Elaine Humphrey,
Biosciences EM Facility
Biosciences Building
Univ. of British Columbia
6270 University Blvd.
Vancouver, BC, V6T-1Z4
Phone: 1-604.822-3354
FAX: 1-604.265-5315
Email: ech-at-unixg.ubc.ca.


Application deadlines:

Application forms are due by March 1, 2000!
Deadlines are early to facilitate setting up groups. Successful applicants
will be notified by April 1, and a deposit of 50% must be received by April
15, 2000 to reserve your position. In general, deposit refunds are only be
possible if your position can be filled from the waiting list. The
remainder of the fees is due at registration.

DATES

Applications must be received by March 1, 2000
Deposit due April 15 2000
Registration 5:00 - 7:00 PM Sunday, June 18, 2000
First Lecture 7:30 PM Sunday, June 18, 2000
Live-cell Course ends, noon Thursday, June 29, 2000


TEACHING PHILOSOPHY

As befits teaching in an area at the boundary of "what is now known,"
lecturers have been chosen based on their expertise as scientists working
in the field rather than because they all agree. They are encouraged more
to be provocative than to be prosaic. Students should expect discussion in
areas where differences of opinion exist.

Prior to the course, students will be organized into groups and encouraged
to communicate by email/phone, about the "Living-cell" group projects that
they will pursue during the course and that will be presented to the class
on the last day of the course. It has been found that group interactions
make best use of students' prior experience and can be very effective in
teaching the practical skills covered in a hands-on course of this type.

ARRANGEMENTS FOR LIVE SAMPLES

Students must contact Dr. Elaine Humphrey to make necessary arrangements
for the transport and maintenance of cell lines etc. needed for their
projects. Organisms linked in any way with human disease are not permitted
because of safety considerations. Transport and customs arrangements for
living specimens are entirely the responsibility of the student.
Students also attending the 3D Image Processing Course, may be able to
analyze, process and display some of the 3D data collected from their
specimens


********************************************************************************

Fourth Annual

3D MAGE PROCESSING WORKSHOP

The workshop will cover 3D image processing for measurement and display.
Enrollment is limited to those attending the 3D Microscopy course.
Tuition : $850 US (lunches and snacks incl.)

WHO SHOULD ATTEND?

The course is designed for biologists working with multidimensional and
possibly multicolor microscopical data sets. Getting the data is only half
of the battle. Image data in 3, 4 or even 5 dimensions may be difficult to
store let alone analyze or display. This course is to help students
understand the hardware and software aspects of this problem and give them
the techniques they need to make the best use of their data.

The course is designed for biologists who need to make measurements on 3D
microscopical data sets and then display the results in an effective
manner. The course will be taught on SGI, Macintosh and IBM-compatible
computers. A wide variety of software designed for the 3D microscopy market
will be described, demonstrated and available for use.

Workshop Organizers

o Andreas Kriete Giessen University, DE
o Felix Margadant University of Sydney, Au

Faculty

o Ping Chin Cheng State U. of New York, Buffalo
o Chris MacLean Vaytek Inc., IA

PLAN OF INSTRUCTION
Classes will meet from 8:30-12:00 and 1:00-6:00 with lecture-demonstrations
followed immediately by hands-on laboratory sessions using a variety of
workstations. Students will "learn-by-doing" with two to a machine. Lab
handouts will describe some specific exercises to be performed on "canned"
data sets. Facilities and supervision will be available until 11:00 PM, for
students to work on their own data.
****************************************
Prof. James B. Pawley, (on Sabbatical) Ph. 61-2-9351-7548/2351
Room LG 10, Madsen Building, F-09, FAX 61-2-9351-7682
University of Sydney, NSW, 2006 Australia JBPAWLEY-at-FACSTAFF.WISC.EDU
Come to the 5th 3D Live-cell Microscopy Course, June 19-29, 2000, UBC, Canada.
Info and forms at: http://www.cs.ubc.ca/spider/ladic/course/bulletin.html
"A scientist is not one who can answer questions but one who can question
answers." Theodore Schick Jr., Skeptical Enquirer, 21-2:39



From daemon Fri Feb 04 22:50:55 2000



From: Stan Rice :      srice-at-alpha.utampa.edu
Date: Fri, 4 Feb 2000 21:55:32 -0600
Subject: LKB Nova ultramicrotome

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I recently inherited a used LKB Nova ultramicrotome from a medical
facility in town. The previous owner could not locate the operating
instructions and I am at a loss to figure out its proper operation. Does

anyone on the list have instructions for operation of this instrument??
I would be willing to reimburse anyone for a copy of the instruction
manual.

Dr. Stanley A. Rice
University of Tampa
srice-at-alpha.utampa.edu
(813) 253-3333 Ext. 3340

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From daemon Fri Feb 04 22:50:57 2000



From: Patricia A. Glazebrook :      PGlazebr-at-Research.metrohealth.org
Date: Fri, 4 Feb 2000 21:57:44 -0600
Subject: Cy5 filtercube today on a upright flourescent microscope

Contents Retrieved from Microscopy Listserver Archives
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I tried a Cy5 filtercube today on a upright flourescent microscope equiped
with a Diagnostics Instrument Spot Camera. My tissue had been
immunolabelled with Cy5 and I did not expect to see thru the microscope. So
we took pictures with the Spot Camera and the immunolabeling seemed to
work. The problem was that there was uneven illumination in the pictures,
half moon of brightness and the rest of the field dark. The salesperson did
check that the mercury bulb was aligned correctly. Also there was no
ambient light from the room causing the shadow.

Anyone with an idea  of what is causing this problem?

Thank-you, Pat Glazebrook





From daemon Fri Feb 04 22:50:56 2000



From: Lucille A. Giannuzzi :      lag-at-mail.ucf.edu
Date: Fri, 4 Feb 2000 21:59:21 -0600
Subject: TEM Specimen Prep short course

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


TEM Specimen Preparation Short Course!!!!

With emphasis on Tripod Polishing and Focused Ion Beam Specimen Preparation
....

..will be offered in conjunction with the Joint Annual Meeting of the
Florida Society for Microscopy and the Florida American Vacuum Society in
Orlando at the University of Central Florida March 15-17, 2000.

Instructors:

Ron Anderson, IBM
Fred Stevie, Lucent Technologies
Lucille Giannuzzi, UCF

Vendor Sponsors:

FEI Company
Micro Optics
South Bay Technology

For registration material please contact Lucille Giannuzzi at lag-at-mail.ucf.edu

*******************************************************************
Lucille A. Giannuzzi, Ph.D.

Associate Professor, Dept. of Mechanical, Materials, and Aerospace Eng.,
University of Central Florida,
PO Box 162450, 4000 Central Florida Blvd., Orlando, FL 32816-2450 USA
phone (407) 823-5770 fax (407) 823-0208 email lag-at-mail.ucf.edu

Director, UCF/Cirent Materials Characterization Facility, 12443 Research
Parkway, Suite 305
Orlando, FL 32826 phone (407) 275-4354,5,6 fax (407) 275-4321
--------------------------------------------------------------------
"Good judgement comes from experience.

Experience comes from making bad judgement."

Mark Twain
********************************************************************




From daemon Sun Feb 06 18:27:19 2000



From: Roger Moretz :      rcmoretz-at-excite.com
Date: Fri, 4 Feb 2000 20:52:16 -0800 (PST)
Subject: Re: Help:A comparison of optics

Contents Retrieved from Microscopy Listserver Archives
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The responses to this thread have been outstanding. Yet from my perspective
the unasked and thus unanswerable question is: what is the purpose for the
lens? A couple of the responses have brushed the issue, but it still hasn't
been specifically stated. Assuming that resolution is the main issue, the
concerns of flatness of field, NA, color correction, working distance, etc,
all have to be factored into the evaluation of the different lenses. It
should be possible to demo both (or any of a set of) lenses under the lab
and experimental conditions of real life. Only then can one truly determine
which lens is the "best" (or more correctly, the appropriate) lens. All of
the theory and specifications in the world don't matter if the lens doesn't
aid the microscopist in obtaining the data necessary to carry out the work.
One thing that is unalterable is that even if one is considering a number of
"infinity-corrected" lenses from different manufacturers, they may not be
truly interchangeable. Make sure the lens works on the microscope body.
Then make sure it provides the performance needed. That's the right lens.

Roger Moretz


On Thu, 3 Feb 2000 16:04:55 -0600, Steve Niemela wrote:

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}
} To: {Microscopy-at-MSA.Microscopy.Com}
} Subject: A comparison of optics
} Date: Thu, 3 Feb 2000 10:57:19 -0600
} MIME-Version: 1.0
} Content-Type: multipart/alternative;
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} X-Mailer: Microsoft Outlook Express 5.00.2615.200
} X-MimeOLE: Produced By Microsoft MimeOLE V5.00.2615.200
}
} A client of ours wonders about a comparison between Zeiss and
Olympus
} optics. The Zeiss 100X lense cost $10,000, and the Olympus about
$4,000:
} does that mean the Olympus is inferior?  That's what our client's
} colleagues imply. What measure of lense function is relevant? Does
Olympus
} (or Zeiss) have a specification, like 'resolves particles to 0.1 micron'
} or something like that?  Is there a measure of distortion? Best
} regards, Steve NiemelaVayTek, Inc.305 West Lowe AvenueFairfield, IA
} 52556email: sniemela-at-vaytek.com Phone: 515.472.2227Fax: 515.472.8131web:
} www.vaytek.comgeneral email: vaytek-at-vaytek.com
}
}
}


Roger C Moretz, Ph.D.





_______________________________________________________
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From daemon Sun Feb 06 18:27:43 2000



From: Ken Tiekotter :      tiekotte-at-up.edu
Date: Sat, 5 Feb 2000 00:36:00 -0800 (PST)
Subject: Re: Aqueous mounting medium

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Lesley,
How about CMC (Refractive Index=1.37). CMC10 (R.I.=1.39), CMCP-9
(R.I.=1.40) and CMCP-10 (R.I. 1.41)? This is a mixture of Polyvinyl
alcohol (5%), phenol(25%), lactic acid (25%), and water (45%).

I believe you can get this from Polysciences Inc. This mountant causes
problems with most stained specimens, but may work for you. Is glycerine
jelly auto-fluorescening?

-Ken

--------------
Ken Tiekotter
Dept. of Biology
The University of Portland
5000N. Willamette Blvd.
Portland, OR 97303





From daemon Sun Feb 06 18:27:45 2000



From: Ken Tiekotter :      tiekotte-at-up.edu
Date: Sat, 5 Feb 2000 00:45:21 -0800 (PST)
Subject: Re: Help:A comparison of optics

Contents Retrieved from Microscopy Listserver Archives
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Dan,
Mixing and matching objectives is one thing, while doing the same for
oculars is probably not visually as big an issue. However, in highly
corrected systems, compensating eyepieces are a critical consideration if
one wishes to maintain image quality, i.e., plan apo objectives require
compensating oculars to maintain the correction of the apochromatic
objective. This is especially true for color reproduction in color
photomicrograph. This is not as important in black and white
photomicrography.

-Ken

----------------
Ken Tiekotter
Dept. of Biology
The University of Portland
5000 N Willamette Blvd.
Portland, OR 97303


On Fri, 4 Feb 2000, Dan Freidus wrote:

} ------------------------------------------------------------------------
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} -----------------------------------------------------------------------.
}
}
} -----Original Message-----
} } From: Ken Tiekotter {tiekotte-at-up.edu}
} ..One does not mixed and match objectives from different systems merely for
} the convenience of pricing....
} ----------------------------------------------------------------------------
} ---------------------------------------------------------------------------
}
} Of course, I've heard this sort of statement many times before. But has
} anyone ever done any comparisons of mixed systems to see if there are
} certain brands that are particularly incompatible or particularly
} compatible?
}
} I ask this both as a general question and because I use a Wild M7 for which
} I need a pair of high eyepoint oculars. I don't need the adjusting collar
} built into current models and haven't been able to find an older pair used.
} But before I buy a Nikon/Olympus/ etc. pair I'd love to know how different
} the complete system will be from what I've got now.
}
} I know many people who have used third party photo eyepieces, in just the
} situation where you'd think quality would be of the utmost concern. Dealers
} are often not the best source of info since they tend to represent one brand
} and, even when they are completely honest, rarely have experienc ewith a
} wide range of brands (especially mixed up.
}
} Dan Freidus
} freidus-at-wwnet.com
}
} P.S. Of course, if anyone has a pair of Wild 10x high eyepoint oculars for
} sale, that would also solve my problem (I'd also consider other
} magnifications). (I'm also looking for accessory objective lenses and
} various other accessories. Please contact me off-list if you've got anything
} for sale or trade.)
}
}
}
}
}
}



From daemon Sun Feb 06 18:28:48 2000



From: Paul Webster :      pwebster-at-hei.org
Date: 05 Feb 00 12:50:25 -0800
Subject: RE: Aqueous mounting medium

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Lesley Weston {lesley-at-interchange.ubc.ca}
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From: Paul Webster :      pwebster-at-hei.org
Date: 05 Feb 00 12:50:25 -0800
Subject: RE: Aqueous mounting medium

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The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Reply to: RE: Aqueous mounting medium
Dear Lesley,

You can make a very useful mounting medium from inexpensive ingredients.
The main ingredient is poly(vinyl) alcohol which is soluble in water but will dry without shrinking too much. The water solubility means you can take previously mounted slides, rehydrate them and use them again.

Fading of fluorescent dyes can be retarded by including anti-fade compounds.

You can find the recipe at {http://www.hei.org/htm/moviol.htm} .

Details of anti-fade compounds can be found here (at the bottom of the page) {http://www.hei.org/htm/ifse.htm} .
Ths site is getting a little outdated so if you feel there are details missing then please contact me with your questions.

Many thanks to Heinz Schwarz of Tuebingen for writing these protocols.

Regards,

Paul Webster

Paul Webster, Ph.D.
Associate Scientist & Director
Ahmanson Advanced Electron Microscopy & Imaging Center
House Ear Institute
2100 West Third St.
Los Angeles, CA 90057

Phone: (213) 273-8026
Fax: (213) 413-6739
e-mail: pwebster-at-hei.org
http://www.hei.org/htm/aemi.htm



From daemon Sun Feb 06 18:29:32 2000



From: donald j marshall :      dmrelion-at-world.std.com
Date: Sun, 6 Feb 2000 07:21:55 -0500 (EST)
Subject: solid state emitters

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"Dr. Gary Gaugler" {gary-at-gaugler.com}
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I would call this a typical PARTIAL judgment.
What you see is what you get. Thee only way, to the best of my experience,
is to get both objectives, side by side if possible, and to do your own
evaluation. I have honestly doubts concerning those values set on
objectives.
Things change and optic too and sometimes faster that we could expect.
Norm
----- Original Message -----
} From: Dr. Gary Gaugler {gary-at-gaugler.com}
To: MSA listserver {Microscopy-at-sparc5.Microscopy.Com} ; {sniemela-at-vaytek.com}
Sent: Friday, February 04, 2000 5:02 PM


I am interested to know what the present state of the art is re: solid state
electon emitters, total electron beam currents available and also beam
current densities. If anyone could point out a recent review article, I
would appreciate it. Thanks,

Don Marshall

Donald J. Marshall
Relion Industries
P.O. Box 12
Bedford, MA 01730
Ph: 781-275-4695
FAX: 781-271-0252
email dmrelion-at-world.std.com

Cathodoluminescence, mass spectroscopy, electron beam technology


"A weed is a flower out of place."

(Please note: Do not send email with attachments to this address. Instead, send it to donbarlen-at-aol.com. Thank you.)



From daemon Sun Feb 06 18:29:52 2000



From: Bill Miller :      microbill-at-mohawk.net
Date: Sun, 06 Feb 2000 08:43:16 -0500
Subject: Re: Help:A comparison of optics

Contents Retrieved from Microscopy Listserver Archives
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In fact, if you're really interested in getting the BEST lens, try at
least three examples of the ones you are interested in . All high NA lenses
must meet some minimal standard but some are significantly better than that
minimum and the only way to find one that is is to try THE lens you are
going to buy - not just an example.

Bill Miller

At 10:04 PM 2/5/00 -0400, Norm wrote:
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From daemon Sun Feb 06 18:31:08 2000



From: VALLARAY-at-aol.com
Date: Sun, 6 Feb 2000 16:10:45 EST
Subject: Re: Electron diffraction simulations

Contents Retrieved from Microscopy Listserver Archives
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Erik,

Do not have difraction sofrware, but here are some URLs where you can
download free simulation software I use for e-beam to solid interactions,
hope it may be useful for you.

Casino is a freeware for monte carlo simulations:
http://www.gme.usherb.ca/casino/

Monte carlo resources:
http://dsa.dimes.tudelft.nl/pattern_definition/monte/monte.html

My FAVORITE monte carlo software:
http://web.utk.edu/~srcutk/htm/simulati.htm

Valery Ray, MSEE,
SEM engineer
Applied Materials
001-208-8413744



From daemon Mon Feb 07 17:07:17 2000



From: Richard Hey :      richardh-at-jeoleuro.com
Date: Sun, 6 Feb 2000 18:32:21 -0600
Subject: Re: Parallel beam

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Hi everybody
While this has been an interesting discussion we seem to have got away from
the original question.

This is the method I use - after of course ensuring normal alignments and
adjustments have been carried out

Insert the objective aperture with a specimen in the beam. Focus the image
of this aperture in Selected Area Diffraction using the first intermediate
lens focus.

Focus the diffraction pattern (i.e. the smallest zero order spot) with the
final condenser lens, this will give a parallel beam incident upon the
specimen.

To test this, remove the objective aperture and insert the largest selected
area aperture. If the beam is parallel moving this aperture will not move
the diffraction spot.


------------------------------------------------------------

Tilt and Shift alignment wobblers in the 2010

Tilt - The purpose of this alignment is to ensure that when the beam is
tilted it doesn't move on the specimen. This is double tilt correction and
depends on using double deflector coils.

Shift - Double shift correction is meant to ensure that when the beam is
shifted it doesn't tilt. The operation of this alignment is dependent upon
the value of the first intermediate lens. The best value for this alignment
is a parallel beam condition as described above. By the very nature of the
beast it will be found to be impossible to perform this alignment correctly
at some CBD conditions. This is meant to be a convergent beam mode after all
not a parallel beam mode.

I understand that this is also referred to as tilt and shift purity.

Richard Hey

The views expressed herein are purely my own and do not reflect those of my
employer

Richard Hey
Technical Support Engineer
Jeol (UK) Ltd
Phone: (44) 1707 377117




From daemon Mon Feb 07 17:07:20 2000



From: Qiaoling Jin :      jinq-at-pilot.msu.edu
Date: Sun, 6 Feb 2000 20:37:18 -0500 (EST)
Subject: Immnogoldlabeling on TEM

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Dear colleagues,
I am now currently using TEM to observe a filamentous structure
so-called hrp pilus produced by plant pathogen, Pseudomonas syringae which is
grown on planta mimic medium agar plates. Even though I could easily find this
structure through directly put the nickel-grid on the bacteria plate for a
certain time,followed by the negative staining, I can't convince other people
to believe it's the structure we are looking for as its size is around
7-10 nm, which is indistinguishable from that of bacterial flagellum.
So we want to label this structure by using the specific antibody against
its major structural protein followed by the 10 nm gold-conjugated second
antibody. The difficulty I am encountering now is most pili are washed away
after the whole immnuogoldlabeling process. Since I am a beginnner on TEM work,
I don't know whether it's a very commonly encountered problem in
immnogoldlabeling experiment or just a very special case happened to me, such
as the pilus i'm interested in is too vulnerable to survive the exclusive wash,
or I didn't master the whole immunogoldlabeling techinique. If it's a common
problem, I hope someone could kindly provide me with your valuable experience
on how to resolve this problem, how we can prevent them to be washed away. If
it's due to my techinical problem, could you please send me a very detailed
immunogoldlabeling protocol.
Any kind of help will be highly appreciated
have a nice week
yours
qiaoling jin
jinq-at-msu.edu






From daemon Mon Feb 07 17:08:36 2000



From: Augusto_A_Morrone-at-notes.seagate.com
Date: Mon, 7 Feb 2000 08:17:13 -0600
Subject: Re: TEM: Jeol 2010 question

Contents Retrieved from Microscopy Listserver Archives
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Jonathan, Andrew, Richard, Larry:

Thank you for your responses. Please note that I do perform the TILT and SHIFT
alignments (aka "pivot point alignment" in other instruments) on a regular
basis, but this doesn't solve the problem.

Regarding: "...What did you mean by a voltage centre? My interpretation of this
is that
the source high tension is wobbled and any shift in the (previously
focussed) image is minimised by tuning the beam tilts. This alignment would
be used for energy filtered TEM (EFTEM) when doing elemental mapping on a
Gatan Imaging Filter (GIF) for example".

This is a (standard) routine alignment for imaging as well. The voltage center
alignment is done by first focusing the specimen at the optimum OL current
(adjusting Z), then adjusting the Tilt (in image mode) and Shift (in diffraction
mode), and finally adjusting the beam tilts to minimize the image shift at the
center of the screen. The problem arises when the Brightness is changed, as is
typically adjusted when optimizing the illumination on the sample to record an
image (intense illumination) versus an SA diffraction pattern (fully overfocused
condenser). Apparently this brightness change affects the voltage center as
evidenced by a shift of the direct beam as that brightness change is effected in
the SAD mode. This shift in the direct beam is equivalent to a beam tilt.

Augusto




From daemon Mon Feb 07 17:08:38 2000



From: L. D. Marks :      ldm-at-apollo.numis.nwu.edu
Date: Mon, 7 Feb 2000 08:30:10 -0600 (CST )
Subject: Re: Parallel beam

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I regret to say that there are some important points which perhaps are
being missed. Classic optics, i.e. most TEM textbooks and introductory
optics texts are for one of two cases:
a) Every point in the condensor aperture (CA) is incoherent (i.e is not
related in phase in a statistical sense) with respect to any other point.
b) Every point in the CA is coherent (i.e. has a fixed phase) with
respect to any other point.

Approximation a) is used in most computer codes for HREM, approximation
b) for STEM.

Alas, both a) and b) are only approximations! Current microscopes,
particularly FEGs (but perhaps others as well) operate under conditions
when neither of the two approximations are valid. While the analytic
theory is well established (see the Chapter in Born and Wolff on
partial coherence), it is not easy and there are no clean solutions
that you can write down. (It can be solved numerically, but this
would require big 4-D FFT's which are beyond most current computers.)

The simplest test is to fully focus the illumination. If the spot
size is A nm, the coherence in the CA is about 1/A nm-1 (which you can
convert to an angle as appropriate). If this is small relative to the
CA radius you are working with case a) above. If it is about the same as the
CA radius you have b). If it is 2-3 times less, neither approximation
is correct!

The moral of the story:
1) If you have a), you can simulate an HREM image with confidence
using any of the available programs. They are WRONG quantitatively
otherwise.
2) If you have a), you can use simple "ray-diagram" optics,
but not otherwise.
3) If you have b) the simple equations for STEM operation are
valid, but not otherwise.

Sorry, but as microscopes improve the theory we need to understand them
can change.

++++++++++++++++++++++++++++++++++++++++++++++++
Laurence Marks
Department of Materials Science and Engineering
Northwestern University
fax: (847) 491-7820
mailto:l-marks-at-nwu.edu
http://www.numis.nwu.edu
++++++++++++++++++++++++++++++++++++++++++++++++



From daemon Mon Feb 07 17:08:40 2000



From: Michael Herron :      herro001-at-maroon.tc.umn.edu
Date: Mon, 07 Feb 2000 09:20:44 -0600
Subject: Diameter of human RBC?

Contents Retrieved from Microscopy Listserver Archives
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All,

I am trying to get a handle on the scale of some old pictures. There
are human RBC in theses pictures. Can anyone tell me the diameter of
your average human RBC?

Thanks
Mike

--
_________________________________________________
/ Michael J. Herron /
/ U of MN,Medicine/Infectious Diseases /
/ herro001-at-maroon.tc.umn.edu /
/ http://128.101.243.213 /
/_____________________________________________/



From daemon Mon Feb 07 17:09:09 2000



From: Walck. Scott D. :      walck-at-ppg.com
Date: Mon, 7 Feb 2000 10:52:29 -0500
Subject: Be X-ray peaks -I don't think so

Contents Retrieved from Microscopy Listserver Archives
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When a characteristic X-ray is given off from an atom, it carries 1 h-bar
(Planck's constant divided by 2 pi) of angular momentum. The electronic
transition that occurred for the X-ray to come off must conserve angular
momentum of the system. Therefore, a transition from a 2s1/2 to a 1s1/2
state is forbidden by selection rules. You can't produce a Be X-ray.
-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)




From daemon Mon Feb 07 17:09:24 2000



From: A. K. Christensen :      akc-at-umich.edu
Date: Mon, 07 Feb 2000 11:38:52 -0500
Subject: Re: Diameter of human RBC?

Contents Retrieved from Microscopy Listserver Archives
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Mike: According to Fawcett's CONCISE HISTOLOGY (page 32), "In the blood,
erythrocytes are 8.5 micrometers in diameter, but in the dehydrated state,
in dried blood smears, they measure 7.6 micrometers." --Kent

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
A. Kent Christensen, Professor Emeritus
Department of Cell and Developmental Biology, Medical Science II Building
University of Michigan Medical School, Ann Arbor, MI 48109-0616
Office: 2703A Medical Science II Building
Tel (work) (734) 763-1287, Fax (work) (734) 763-1166
E-mail: akc-at-umich.edu
http://www.umich.edu/~akc/
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
--On Mon, Feb 7, 2000 9:20 AM -0600 Michael Herron
{herro001-at-maroon.tc.umn.edu} wrote:

} All,
}
} I am trying to get a handle on the scale of some old pictures. There
} are human RBC in theses pictures. Can anyone tell me the diameter of
} your average human RBC?
}
} Thanks
} Mike
}
} --
} _________________________________________________
} / Michael J. Herron /
} / U of MN,Medicine/Infectious Diseases /
} / herro001-at-maroon.tc.umn.edu /
} / http://128.101.243.213 /
} /_____________________________________________/






From daemon Mon Feb 07 17:09:45 2000



From: donald j marshall :      dmrelion-at-world.std.com
Date: Mon, 7 Feb 2000 12:06:10 -0500 (EST)
Subject: Re: Computer lock-ups from static

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



John, If they do, I hope that they will respond to the whole list because we
have clients who have experienced similar problems, especially re: computers
that are controlling VIS spectrometers collecting CL spectra. I know that
there are in line RF filters available (e.g., Steward Co.) and there may be
others but the final word is not in on how well these work yet.

Don Marshall


} From microprobe-at-microanalysis.org Mon Feb 7 11:43:28 2000
} }
} From: John Hunt {hunt-at-ccmr.cornell.edu}
} Subject: Computer lock-ups from static
} To: microprobe-at-microanalysis.org
}
}
} Dear Probe community,
} It is winter in Central NY, and the room humidity is 33%. Static
} electricity (I believe) has been causing frustrating computer lockups
} requiring rebooting our microprobe workstation.
} I am wondering if anyone has found the best solution for preventing
these
} problems.
}
}
}
}
} John Hunt
} Cornell Center for Materials Research
} 607-255-0108 microprobe
} -3789 SEM/TEM
} -2365 fax
}

Donald J. Marshall
Relion Industries
P.O. Box 12
Bedford, MA 01730
Ph: 781-275-4695
FAX: 781-271-0252
email dmrelion-at-world.std.com

Cathodoluminescence, mass spectroscopy, electron beam technology


"A weed is a flower out of place."

(Please note: Do not send email with attachments to this address. Instead, send it to donbarlen-at-aol.com. Thank you.)



From daemon Mon Feb 07 17:09:49 2000



From: Marisa Ahmad :      mahmad-at-semiconductor.com
Date: Mon, 7 Feb 2000 12:33:38 -0500
Subject: tungsten filaments

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I would like to tap the wealth of information out there.

We are experiencing a lot of problems with the tungsten filaments that we
are currently using on our JEOL 5800 SEM. During long acquisitions (many
hours) there is a lot of brightness drift and shifting, even when lengthy
stabilization periods are given prior to the acquisitions. JEOL has
checked out the SEM itself, and can't find the source for the drifting. I
was thinking that it might be the filament. I was wondering if there are
different types of tungsten filaments out there, and which is the most
stable over long periods of time. I would like to try out all of them
(considering trying to switch our entire system to a LaB6 filament is a bit
much for now). Any recommendations or experiences you have would help us
out a lot.

Thanks in advance!


Marisa Ahmad
R&D Specialist
Semiconductor Insights
mahmad-at-semiconductor.com


weather report (for those interested):
It's unusually sunny and warm for here in Ottawa.....only -10 degrees C (but
it feels like -25 with the wind). It's such a nice day that I washed my
car this morning since it's not really supposed to be brown - now all the
doors and windows are frozen shut. {sigh}



From daemon Mon Feb 07 17:10:07 2000



From: Marie E. Cantino :      cantino-at-uconnvm.uconn.edu
Date: Mon, 7 Feb 2000 12:45:54 -0500
Subject: Re: Diameter of human RBC?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In my experience, RBCs prepared for SEM by critical point drying or HMDS
shrink even more and may be only 4 or 5 microns across.

Marie
}
} Mike: According to Fawcett's CONCISE HISTOLOGY (page 32), "In the blood,
} erythrocytes are 8.5 micrometers in diameter, but in the dehydrated state,
} in dried blood smears, they measure 7.6 micrometers." --Kent
}

Dr. Marie E. Cantino
Dept. of Physiology and Neurobiology, U-2131
University of Connecticut
Storrs, CT 06269-2131
Phone: 860-486-3588
Fax: 860-486-1936




From daemon Mon Feb 07 17:10:10 2000



From: Karl E. Garsha :      keg-at-csd.uwm.edu
Date: Mon, 7 Feb 2000 12:03:51 -0600 (CST)
Subject: Re: Immnogoldlabeling on TEM

Contents Retrieved from Microscopy Listserver Archives
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------------- Begin Forwarded Message -------------



------------- End Forwarded Message -------------




From daemon Mon Feb 07 17:10:12 2000



From: Karl E. Garsha :      keg-at-csd.uwm.edu
Date: Mon, 7 Feb 2000 12:10:39 -0600 (CST)
Subject: Re: Diameter of human RBC?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


It depends on the fixation/dehydration parameters. Somewhere in the
nieghborhood of 4 microns, dependant on the angle of measurement and the angle
of the sample with respect to the camera.
} Date: Mon, 07 Feb 2000 09:20:44 -0600
} From: Michael Herron {herro001-at-maroon.tc.umn.edu}
} X-Accept-Language: en
} MIME-Version: 1.0
} To: Microscopy listserv {Microscopy-at-sparc5.Microscopy.Com}
} Subject: Diameter of human RBC?
} Content-Transfer-Encoding: 7bit
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Mon Feb 07 17:10:18 2000



From: Margaret Brannigan :      brannign-at-asrr.arsusda.gov
Date: Mon, 7 Feb 2000 13:25:52 -0500
Subject: TEM: What is Brandes dip?

Contents Retrieved from Microscopy Listserver Archives
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Hi,

Can anyone tell me what a Brandes dip is and how it is done? The only thing
I know is that it can be used to detect virus particles from plant sap but
I can't find any references on it.

Thank You!

Margaret

Margaret Dienelt

Plant Pathologist
Electron Microscopy Lab

Floral and Nursery Plants Research Unit
U.S. National Arboretum/Agricultural Research Service/USDA

B. 010A, Rm. 238, BARC-W
10300 Baltimore Avenue
Beltsville MD. 20705 USA

(301) 504-6097
Fax: (301) 504-5096




From daemon Mon Feb 07 17:10:30 2000



From: Hao Li :      haoli-at-eng.umd.edu
Date: Mon, 07 Feb 2000 13:56:57 -0500
Subject: Simulation of dislocation network

Contents Retrieved from Microscopy Listserver Archives
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Does anybody know any program capable of doing TEM simulation of
dislocation network, e.g., misfit dislocations in plane view TEM? I know
some programs can do one or two dislocations, but what I need is the
simulation for a set of dislocations.
Thanks a lot.

Hao Li



From daemon Mon Feb 07 17:10:31 2000



From: Steve Chapman :      PROTRAIN-at-CompuServe.COM
Date: Mon, 7 Feb 2000 13:59:53 -0500
Subject: Automatic Microscope and Coherence

Contents Retrieved from Microscopy Listserver Archives
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Hi

It has been very interesting reading about diffraction and electron beams
but it is quite clear that we often give the instrument credit, "the
instrument does it automatically", when credit is not due. Almost every
"automatic" is a designers estimate or better still a calculation, it is a
setting!

After 36 years with TEM I have to say that the only area that the
instrument could set in the SA mode could be the approximate position of
the first image plane. We normally move to the SA mode to free up the
diffraction lens (1st intermediate) to enable it to be focussed on the
diffraction (intermediate) aperture independent of the magnification
system. We would then focus the image inside the aperture with the
objective lens to ensure that the selected area IS the area in the
diffraction pattern. Without this procedure electron rotation and
misalignments could give you a diffraction pattern from an area not in the
centre of the screen! Users are correct in that if they set the eucentric
point or better still, adjust the specimen height to focus at the same
objective lens current value, that done the first image plane will be at
the same point within the diffraction (intermediate) lens.

So how could the Philips work? I would guess they set the diffraction lens
at approximately the first image plane. Then as they do not use it within
their lens scheme for the SA magnifications it is fixed at this focus, good
enough I think?

A second point, parallel beams are very important if you are chasing the
best image quality, biology or materials, most times we need high
coherence. There are misunderstandings on how to obtain a parallel beam or
high coherence, setting the final condenser underfocus is incorrect. The
procedure for high coherence would be to use the smallest spot size you can
tolerate (this probably means you must up the emission current). Once in
this condition overfocus the final condenser (clockwise from crossover) the
spot, what ever size it is now, becomes your virtual source. The further
overfocus you go the greater the beam coherence and that is what we are
after. You will deduce the smaller the condenser aperture the sharper the
spot and the smaller the spot the greater the coherence for a given degree
of overfocus.

Work with a design team and they expect everyone to overfocus, they expect
everyone to use small condenser apertures and they expect everyone to use
small spot sizes. They do not expect everyone to use too low an emission
current (almost everyone does, we have talked before about filament life
being the most important feature of many laboratories) because this makes
the task too difficult!
There must be people more knowledgeable than I who will cut out all the
guesswork?

Steve Chapman
Senior Consultant
Protrain
Electron microscopy courses world wide
http://www.emcourses.com
Tel & Fax 44 (0) 1280 814774



From daemon Mon Feb 07 17:11:18 2000



From: Kalman Rubinson :      kr4-at-is2.nyu.edu
Date: Mon, 7 Feb 2000 14:27:14 -0500 (EST)
Subject: Re: Diameter of human RBC?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On Mon, 7 Feb 2000, Michael Herron wrote:

} I am trying to get a handle on the scale of some old pictures. There
} are human RBC in theses pictures. Can anyone tell me the diameter of
} your average human RBC?

When I was a lad...... in grad school, we were told it was
a useful reference at 7um diameter.

Kal



From daemon Mon Feb 07 17:11:30 2000



From: Joseph Passero :      jp-at-spacelab.net
Date: Mon, 07 Feb 2000 15:15:13 -0500
Subject: ANNOUNCEMENT Course on Polarized Light Microscopy

Contents Retrieved from Microscopy Listserver Archives
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New York Microscopical Society

Bernard Friedman Memorial Workshop

Polarized Light Microscopy

Two consecutive weekends

Saturday, April 21, Sunday, April 22, 2000
Saturday, April 28, Sunday, April 29, 2000

An advanced course on polarized light microscopy which will cover the following topics:
The nature of polarized light
The origin and interpretation of interference colors
Birefringence and crystal orientation
The Indicatrix
Compensation and variable compensators
Interference figures and their interpretation

The workshop will consist of two consecutive weekends of lectures and hands on labs to cover
the theoretical and practical aspects of polarized light microscopy.

The course instructors include;

Jan Hinsch of Leica, Inc.,

John Reffner of Trace Consulting,

N.Y.M.S. Instructor Donald O'Leary.

WHEN: April 22, 23, 28 & 29, 2000 from 10 A.M. to 4 P.M.

WHERE: Location To be Announced.

COST: $275 for N.Y.M.S. members, $295 for non-members (includes membership)
Lunch and course materials are included. Checks made out to N.Y.M.S.

WHO: advanced course for those who have completed "The Use of the Microscope" or
are experienced in microscopy and familiar with the theory of its use.

HOW: Register using the form below. Limited to the first 12 registrants.


FURTHER INFORMATION: Contact Donald O'Leary

eMail: mailto:donoleary-at-worldnet.att.net

(201) 797-8849 Voice Phone Number

PLEASE POST
------------------------------------------------------------------------------------------------------------

Return form to Donald O'Leary, 6 Chittenden Road, Fair Lawn, NJ 07410.

Registration Form

Polarized Light Microscopy, April 22, 23, 28 & 29, 2000

N.Y.M.S. Member _________________ ($275.00) Non-Member __________($295.00)

Name ___________________________________________________________________________

Address _________________________________________________________________________

City _________________________________ State __________________ Zip Code _______________

Phone (W) ____________________________ (H) ___________________________

eMail Address: ______________________________________




________________________________________________________
1stUp.com - Free the Web®
Get your free Internet access at http://www.1stUp.com



From daemon Mon Feb 07 17:12:08 2000



From: Robert Underwood :      underwoo-at-u.washington.edu
Date: Mon, 7 Feb 2000 13:23:54 -0800 (PST)
Subject: Re: Cy5 filtercube today on a upright flourescent microscope

Contents Retrieved from Microscopy Listserver Archives
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Hi Patricia,

I'm just taking a guess. Is it possible, depending on the type of filters
used in the cube, that infrared is passing through as flare if your first
excitation filter is not blocking the IR? Or there may be a coating
problem on your filter? We have an IR blocking filter right after the
mercury lamp. The CCD cameras are extremely sensitive to IR and this has
caused problems on our system. But in your situation I don't know why it
would pass through. So it is probably something else.

Bob
Morphology core
U of Washington

On Fri, 4 Feb 2000, Patricia A. Glazebrook wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} I tried a Cy5 filtercube today on a upright flourescent microscope equiped
} with a Diagnostics Instrument Spot Camera. My tissue had been
} immunolabelled with Cy5 and I did not expect to see thru the microscope. So
} we took pictures with the Spot Camera and the immunolabeling seemed to
} work. The problem was that there was uneven illumination in the pictures,
} half moon of brightness and the rest of the field dark. The salesperson did
} check that the mercury bulb was aligned correctly. Also there was no
} ambient light from the room causing the shadow.
}
} Anyone with an idea  of what is causing this problem?
}
} Thank-you, Pat Glazebrook
}
}
}
}
}



From daemon Mon Feb 07 17:12:10 2000



From: Barbara Foster :      mme-at-map.com
Date: Mon, 07 Feb 2000 16:26:47 -0500
Subject: Re: Help:A comparison of optics

Contents Retrieved from Microscopy Listserver Archives
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One more thought to support Gary's observation about the importance of the
NA of the condenser: The full equation for the limit of resolution is R =
(1.22)(wavelength)/(NAobjective + NAcondenser). However, if you don't oil
it to the back of the slide, you might as well use a lower NA condenser.
Reminds me of the hematology scope built by an otherwise sensible company,
meant to be used in ultra high resolution applications. The manufacturer
was very good about providing a quality, high NA, oil immersion objective
as well as a high NA condenser. The only problem was that you could never
raise the condenser high enough to oil it to the back of the slide and,
therefore, use it at the NA inscribed... only at about .95! What a waste
of time and money!

Also, a reminder that the NAs engraved are the maximum working limit.
Closing down an aperture iris or an iris in the back focal plane of the
objective reduces the NA accordingly.

One last fact: the NA affects both resolution AND edge information, so,
for the crispest, highest resolution images, go for high NA in both
objective and condenser.

Best regards,
Barbara Foster
Consortium President
Microscopy/Microscopy Education ...Educating microscopists for greater
productivity.

125 Paridon Street Suite 102 Springfield, MA 01118
PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com
Visit our web site {http://www.MME-Microscopy.com/education}


At 03:02 PM 2/4/00 -0600, Dr. Gary Gaugler wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Mon Feb 07 17:12:14 2000



From: William Tivol :      tivol-at-wadsworth.org
Date: Mon, 7 Feb 2000 16:35:56 -0500 (EST)
Subject: Re: tungsten filaments

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Marisa Ahmad:
} I would like to tap the wealth of information out there.
}
} We are experiencing a lot of problems with the tungsten filaments that we
} are currently using on our JEOL 5800 SEM. During long acquisitions (many
} hours) there is a lot of brightness drift and shifting, even when lengthy
} stabilization periods are given prior to the acquisitions. JEOL has
} checked out the SEM itself, and can't find the source for the drifting. I
} was thinking that it might be the filament. I was wondering if there are
} different types of tungsten filaments out there, and which is the most
} stable over long periods of time. I would like to try out all of them
} (considering trying to switch our entire system to a LaB6 filament is a bit
} much for now). Any recommendations or experiences you have would help us
} out a lot.
}
Dear Marisa,
Do you have control over the filament voltage? If so, set the
voltage just a hair over that necessary to saturate the filament. If
the voltage is too low, parts of the filament will not emit electrons
which get through the wehnelt and into the beam; if too high, there may
be significant evaporation of the filament; either may cause drifting
and/or shifting. I have had very good luck with the stabilities of
both Agar and Ebtec (now Energy Beam Sciences) filaments; not that
others may not be as good, I haven't tried them. Ebtec makes several
shapes of filament, and one may be best for long-term stability. I
have no interest in Agar or Ebtec except as a satisfied customer.
Yours,
Bill Tivol



From daemon Mon Feb 07 17:12:17 2000



From: Barbara Foster :      mme-at-map.com
Date: Mon, 07 Feb 2000 17:07:29 -0500
Subject: Re: Computer lock-ups from static

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


John,

Have you considered a "local" remedy, like putting up a humidifier? I know
that static can be an issue, but I have never heard of it being this bad
before.
In more humid environments, we often put a small enclosure around a light
microscope then put out a dish of Drierite. Perhaps some simple plastic
sheeting, like they sell for exterior storm windows/wind breaks, can be
hung from the ceiling around your microprobe plus the humidifier will keep
the humidity immediately around your microprobe more balanced.

Best of luck on a tough problem.

Barbara Foster
Consortium President
Microscopy/Microscopy Education ...Educating microscopists for greater
productivity.

125 Paridon Street Suite 102 Springfield, MA 01118
PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com
Visit our web site {http://www.MME-Microscopy.com/education}


At 12:06 PM 2/7/00 -0500, donald j marshall wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Mon Feb 07 17:12:25 2000



From: BLADE1691-at-aol.com
Date: Mon, 7 Feb 2000 17:33:41 EST
Subject: pictures

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




---------- Forwarded message ----------


how can i get a picture of you seal



From daemon Tue Feb 08 16:19:22 2000



From: G. Macdonald :      glenmac-at-u.washington.edu
Date: Mon, 7 Feb 2000 14:52:34 -0800 (PST)
Subject: Re: Cy5 filtercube today on a upright flourescent microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Is this a Spot Jr. or a SpotII? The IR filter in the Jr. cuts off at 650
nm, the SpotII puts it on a slider. Is it all the way out or [partially
occluding the image? Bob Fern
caught a lot of the possible issues, but is your lamp properly focussed
and aligned? Are you picking up light through the eyepieces? We have a
Nikon that will show a semi-circle of light in from a
recessed ceiling light
that projects to the scope at an angle.

Regards,
Glen


Glen MacDonald
Research Scientist
Hearing Research Laboratories of the
Virginia Merrill Bloedel Hearing Research Center
Box 35-7923
University of Washington
Seattle, WA 98195-7923
(206) 616-4156
glenmac-at-u.washington.edu


On Fri, 4 Feb 2000, Patricia A. Glazebrook wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} I tried a Cy5 filtercube today on a upright flourescent microscope equiped
} with a Diagnostics Instrument Spot Camera. My tissue had been
} immunolabelled with Cy5 and I did not expect to see thru the microscope. So
} we took pictures with the Spot Camera and the immunolabeling seemed to
} work. The problem was that there was uneven illumination in the pictures,
} half moon of brightness and the rest of the field dark. The salesperson did
} check that the mercury bulb was aligned correctly. Also there was no
} ambient light from the room causing the shadow.
}
} Anyone with an idea  of what is causing this problem?
}
} Thank-you, Pat Glazebrook
}
}
}
}
}




From daemon Tue Feb 08 16:19:50 2000



From: Michael Bode :      mb-at-soft-imaging.com
Date: Mon, 7 Feb 2000 16:06:21 -0700
Subject: RE: Computer lock-ups from static

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Out here in the dry West (here we have Summer humidity around the 33%
value you mention, in Winter we go a lot further down) we have the same
problem. There are a few methods to help:

1) increase humidity. Perhaps you can use a humidifier in your equipment
room?
2) prevent charging. There are shoes available that prevent charging.
3) get rid of charging. There are several options here:

a) The easiest way and what I usually do if no other method is
available, is to touch some metal part NOT of the PC but a chair or
table before touching the PC. This requires a) that a suitable object is
near the PC, b) that you're not afraid of "shocking" yourself, and c)
that you don't move around a lot while working on the PC.

b) Purchase a grounding mat to place in front of the equipment. these
mats are connected to the AC ground (use the same as the PC ground). If
the mat is big enough so that everybody has to step on the mat first AND
is wearing the conductive shoes, everything should be OK.

c) wear grounded wrist straps. These require of course that you put them
on.

We use a combination of these techniques to protect our equipment both
in the office and the production floor (believe me, it IS necessary
here). As bad as it is for electronic equipment, though, it is just
great for comfort levels outside. 90 degrees (F) feel just right for
bicycling, climbing, rafting...

One company where you can buy this stuff is called "-at-once". Their web
site is www.4atonce.com. I just happened to find their catalog first.
There are many other companies out there that sell static control
equipment. We have no financial interest in this company whatsoever.

Michael

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



} ----------
} From: donald j marshall[SMTP:DMRELION-at-WORLD.STD.COM]
} Sent: Monday, February 07, 2000 10:06:10 AM
} To: hunt-at-ccmr.cornell.edu
} Cc: Microscopy-at-sparc5.Microscopy.Com
} Subject: Re: Computer lock-ups from static
} Auto forwarded by a Rule
}
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America



John, If they do, I hope that they will respond to the whole list
because we
have clients who have experienced similar problems, especially re:
computers
that are controlling VIS spectrometers collecting CL spectra. I know
that
there are in line RF filters available (e.g., Steward Co.) and there may
be
others but the final word is not in on how well these work yet.

Don Marshall


} From microprobe-at-microanalysis.org Mon Feb 7 11:43:28 2000
} }
} From: John Hunt {hunt-at-ccmr.cornell.edu}
} Subject: Computer lock-ups from static
} To: microprobe-at-microanalysis.org
}
}
} Dear Probe community,
} It is winter in Central NY, and the room humidity is 33%.
Static
} electricity (I believe) has been causing frustrating computer lockups
} requiring rebooting our microprobe workstation.
} I am wondering if anyone has found the best solution for
preventing
these
} problems.
}
}
}
}
} John Hunt
} Cornell Center for Materials Research
} 607-255-0108 microprobe
} -3789 SEM/TEM
} -2365 fax
}

Donald J. Marshall
Relion Industries
P.O. Box 12
Bedford, MA 01730
Ph: 781-275-4695
FAX: 781-271-0252
email dmrelion-at-world.std.com

Cathodoluminescence, mass spectroscopy, electron beam technology


"A weed is a flower out of place."

(Please note: Do not send email with attachments to this address.
Instead, send it to donbarlen-at-aol.com. Thank you.)



From daemon Tue Feb 08 16:19:40 2000



From: JoAnn Buchanan :      redhair-at-leland.Stanford.EDU
Date: Mon, 7 Feb 2000 17:24:50 -0600
Subject: GFP antibodies

Contents Retrieved from Microscopy Listserver Archives
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I am planning to try EM immunolabelling with GFP antibodies. Does anyone
out there know of the best source for GFP antibodies and had any luck with
using them for TEM. I would like to try pre-embedding with a nanogold
labelled secondary. Any suggestions would be appreciated. Thank you.

JoAnn Buchanan
Molecular and Cellular Physiology
Stanford University School of Medicine
Stanford, CA 94305
650-723-5856




From daemon Tue Feb 08 16:19:42 2000



From: Frederick Schamber :      fhscham-at-sgi.net
Date: Mon, 07 Feb 2000 19:04:05 -0500
Subject: Re: tungsten filaments

Contents Retrieved from Microscopy Listserver Archives
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Marisa,

Just a quick comment for whatever it's worth. Your posting suggests that you
are concerned about mechanical drifts of the filament -- the subject of a
certain body of folklore in the microscopy community. It's been my experience
that the principal drift in a tungsten filament is not the filament itself, but
rather it is the mechanism supporting the filament which drifts.

The actual issue with drifting filaments is that mechanical stresses in the wire
will gradually relieve under heat, causing the filament to distort slightly.
This is a first-time-used issue. However, I believe most manufacturers include
an annealing step in their process to remove these stresses before the filament
is shipped to a customer (at least, that's what we used to do in another company
when we manufactured our own filaments). In any case, the filament is operated
at a very high temperature and it doesn't take long to anneal out these stresses
under normal use, so long-term drift effects shouldn't be an issue.

My experience is that long-term mechanical drifts originate elsewhere in the gun
mechanism. When you think about it, this makes sense. The filament itself has
very little mass and quickly reaches its ultimate operating temperature
distribution. However, the rest of the gun assembly is much more massive and,
depending on various design considerations, may take hours to reach thermal
equilibrium. Meanwhile, all of those intricate parts are expanding -- and many
at different rates. Principal suspects, in my opinion, are lateral adjusting
and clamping screws -- for example, a set of radial set screws is commonly used
to center the ceramic base of the filament within a ring of some type. I used
to work with a rather complex assembly which positioned the filament via lateral
screws and sometimes had episodes of "filament drift". Subsequently, I have had
nearly a decade of experience with a very simple design which supports the
filament solely via axial pressure and find that "filament drift" isn't an
issue. My conclusion is that a lot of what I used to call "filament drift" was
actually mechanism drift. So if you are experiencing mechanical instabilities
of the filament, I would suspect the way the filament is being supported more
than the filament itself.

This opinion is based on my own experiences, however, and I will be very
interested to hear if others have had different experience.

Fred Schamber
RJ Lee Instruments Limited

Marisa Ahmad wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} I would like to tap the wealth of information out there.
}
} We are experiencing a lot of problems with the tungsten filaments that we
} are currently using on our JEOL 5800 SEM. During long acquisitions (many
} hours) there is a lot of brightness drift and shifting, even when lengthy
} stabilization periods are given prior to the acquisitions. JEOL has
} checked out the SEM itself, and can't find the source for the drifting. I
} was thinking that it might be the filament. I was wondering if there are
} different types of tungsten filaments out there, and which is the most
} stable over long periods of time. I would like to try out all of them
} (considering trying to switch our entire system to a LaB6 filament is a bit
} much for now). Any recommendations or experiences you have would help us
} out a lot.
}
} Thanks in advance!
}
} Marisa Ahmad
} R&D Specialist
} Semiconductor Insights
} mahmad-at-semiconductor.com
}
} weather report (for those interested):
} It's unusually sunny and warm for here in Ottawa.....only -10 degrees C (but
} it feels like -25 with the wind). It's such a nice day that I washed my
} car this morning since it's not really supposed to be brown - now all the
} doors and windows are frozen shut. {sigh}



From daemon Tue Feb 08 16:20:58 2000



From: schmutzm-at-lear.u-strasbg.fr (Schmutz Marc)
Date: Tue, 8 Feb 2000 09:27:07 +0900
Subject: Hematology Slide Stainer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Hi Colleagues around the world,



We're interested in knowing a little bit more about the Hematology Slide
Stainer RSG 61 commercialized by EMS. So if you have one can you please
share your experience with us. Give the plus and also all the minus. If you
have another equivalent brand in which you're fully satisfied of course say
it. Naturally salesman can contact me (offline only please, so the whole
community will not be annoided...)

Thank you for your help


Marc


PS hi Keith how is weather in Plymouth ? Here it's raining and we're singing...





------------------------------
SCHMUTZ Marc
IGBMC
1 rue Laurent FRIES
BP 163
F 67404 Illkirch Cedex
FRANCE

Tel: +33 (0)388 653 330 direct
Fax: +33 (0)388 653 201
email:schmutzm-at-lear.u-strasbg.fr

------------------------------




From daemon Tue Feb 08 16:22:23 2000



From: Bart Cannon :      cannonmp-at-accessone.com
Date: Mon, 07 Feb 2000 16:39:50 -0800
Subject: Solid State Electron Emitters

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Donald,

Nasa has been working on micro-machined solid state electron emitters
and Thomas George of that agency has published a design utilizing an
array of them as the source for a "palm top" SEM in the August, 1998
issue of Nasa Tech Briefs. The proposed SEM relies upon the speculated
use of an electon transparent membrane at the bottom of the lens column
in this sealed, vacuum pumpless system. Resolution is anticipate in the
micron range.

Nasa has also published designs for miniature x-ray tubes in an October
1995 issue of Nasa Tech Briefs.

As far as I know a solid state high energy electron gun exists only as a
Star Wars project.

Bart Cannon
Cannon Microprobe
Seattle
206 522 9233



From daemon Tue Feb 08 21:27:03 2000



From: Bart Cannon :      cannonmp-at-accessone.com
Date: Mon, 07 Feb 2000 16:39:50 -0800
Subject: Solid State Electron Emitters

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Donald,

Nasa has been working on micro-machined solid state electron emitters
and Thomas George of that agency has published a design utilizing an
array of them as the source for a "palm top" SEM in the August, 1998
issue of Nasa Tech Briefs. The proposed SEM relies upon the speculated
use of an electon transparent membrane at the bottom of the lens column
in this sealed, vacuum pumpless system. Resolution is anticipate in the
micron range.

Nasa has also published designs for miniature x-ray tubes in an October
1995 issue of Nasa Tech Briefs.

As far as I know a solid state high energy electron gun exists only as a
Star Wars project.

Bart Cannon
Cannon Microprobe
Seattle
206 522 9233



From daemon Wed Feb 09 16:49:00 2000



From: Bart Cannon :      cannonmp-at-accessone.com
Date: Mon, 07 Feb 2000 16:39:50 -0800
Subject: Solid State Electron Emitters

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Donald,

Nasa has been working on micro-machined solid state electron emitters
and Thomas George of that agency has published a design utilizing an
array of them as the source for a "palm top" SEM in the August, 1998
issue of Nasa Tech Briefs. The proposed SEM relies upon the speculated
use of an electon transparent membrane at the bottom of the lens column
in this sealed, vacuum pumpless system. Resolution is anticipate in the
micron range.

Nasa has also published designs for miniature x-ray tubes in an October
1995 issue of Nasa Tech Briefs.

As far as I know a solid state high energy electron gun exists only as a
Star Wars project.

Bart Cannon
Cannon Microprobe
Seattle
206 522 9233



From daemon Wed Feb 09 16:49:22 2000



From: Barbara Foster :      mme-at-map.com
Date: Mon, 07 Feb 2000 19:57:13 -0500
Subject: LM: Course reminder

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Just a friendly reminder about the upcoming American Chemical Society
Course, "Applied Optical Microscopy". This course is not just for chemists!

If you need a refresher on a basic technique, need to know more about
interpreting images gathered by a variety of contrast systems (including
Hoffman Modulation Contrast and Polarized light), or are moving more into
digital imaging, this is a great course for you!

Hands-on, 3 days of total immersion, basic principles through advanced
techniques, quantitative polarized light.... and New Orleans, thrown in for
good measure! $895 for ACS members; $995 for non-members (tuition and
materials only).

Visit the MME website for details: {http://MME-Microscopy.com/education}

Best regards,
Barbara Foster
ACS Course Coordinator

Microscopy/Microscopy Education ...Educating microscopists for greater
productivity.

125 Paridon Street Suite 102 Springfield, MA 01118
PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com
Visit our web site {http://www.MME-Microscopy.com/education}
******************************************************



From daemon Tue Feb 08 16:20:32 2000



From: Hay, Karen :      KHay-at-ahs.llumc.edu
Date: Mon, 7 Feb 2000 19:13:42 -0800
Subject: RE: Diameter of human RBC?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




---------- Forwarded message ----------



-----Original Message-----
From: Michael Herron [SMTP:herro001-at-maroon.tc.umn.edu]

I am trying to get a handle on the scale of some old pictures.
There
are human RBC in theses pictures. Can anyone tell me the diameter
of
your average human RBC?


Mike-

I have recently subscribed to this list in the hopes of picking up some SEM
pointers as I am beginning to dip my toes into the field. I certainly
can't answer any SEM questions, but I CAN answer your question regarding the
size of human RBCs. On a dried film, normal RBC's have a diameter of
between 7.2-7.9 um. In certain disease states, they can deviate markedly
from this. Microcytic RBC's can be well under 6 um, while macrocytes will
have diameters greater than 9 um.

Good luck in your sizing!

{ {...} }
Karen Hay, MS, MT(ASCP)
Hematology Research, MC 2522
Loma Linda University Medical Center
Loma Linda, CA 92354
Tel: (909) 558-4000 x45350
Fax: (909) 558-4189
Khay-at-ahs.llumc.edu {mailto:Khay-at-ahs.llumc.edu}




From daemon Tue Feb 08 16:20:51 2000



From: COURYHOUSE-at-aol.com
Date: Mon, 7 Feb 2000 23:24:15 EST
Subject: Re: Diameter of human RBC?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


what is a rbc?
in machining we had a theoretical size referred to by the Dimension of a RCH
thanks Ed Sharpe archivist for SMECC



From daemon Tue Feb 08 16:20:57 2000



From: Ken Tiekotter :      tiekotte-at-up.edu
Date: Mon, 7 Feb 2000 22:45:24 -0800 (PST)
Subject: Re: Diameter of human RBC?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Michael,
How about ~7.5 to 8.5um in diameter (depending on fixation/preparation)
and ~2um in greatest thickness.
-Ken

On Mon, 7 Feb 2000, Michael Herron wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} All,
}
} I am trying to get a handle on the scale of some old pictures. There
} are human RBC in theses pictures. Can anyone tell me the diameter of
} your average human RBC?
}
} Thanks
} Mike
}
} --
} _________________________________________________
} / Michael J. Herron /
} / U of MN,Medicine/Infectious Diseases /
} / herro001-at-maroon.tc.umn.edu /
} / http://128.101.243.213 /
} /_____________________________________________/
}
}



From daemon Tue Feb 08 16:21:01 2000



From: Bright, Alan :      ABright-at-brightinstruments.com
Date: Tue, 8 Feb 2000 08:59:57 -0000
Subject: SEM: automotive paints

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Jorgelina,

We have supplied a number of Cryostats & Microtomes to the automotive
industry for sectioning paint finishes on steel body parts & plastic
components e.g. bumper (fenders) door handles, door handle surrounds
etc. Please view our Website to see our range of instruments.

If I can be of further assistance please come back to me.

Best Regards

Alan Bright

Bright Instrument Co.Ltd.
St Margaret's Way
Huntingdon
PE18 6EB
England

Tel No:+44 (0)1480 454528
Fax No:+44 (0)1480 456031
Email: AlanBright-at-brightinstruments.com
Web Site: www.brightinstruments.com



-----Original Message-----
} From: "csedax-at-alpha.arcride.edu.ar"-at-sparc5.Microscopy.Com
[mailto:"csedax-at-alpha.arcride.edu.ar"-at-sparc5.Microscopy.Com]
Sent: 08 February 2000 17:35
To: MICROSCOPY-at-sparc5.microscopy.com


Hi!
My name is Jorgelina C. Altamirano. I'm an undegrated student of
the
licenciate in chemical sciences.
I'm currently preparing my thesis, being its subjet: The
morphological
characterization and the chemical analysis of automotive paints with
forensic
purposes.
I'm undergoing my work using SEM and EPMA techniques with a
JEOL,
JSM 35C and a microprobe EDAX PV9500.
I've chosen some papers as references:
- Beam et al, Analysis Protocol for discrimination for
automotive
paints by SEM-EDAX, Journal of Forensic Sciences, 35, 5, 1990
- Zieba-Palus et al, Mikrochim. Acta, 14, 1997.

I've taken same samples of automotive paints (such as Fiat,
Peugeot,
Renault, Ford, Chevrolet, Volkswagen) corresponding to the years
1994-99.
I've found many difficulties while preparing them in:
- separating the paint from the body work of the car;
- cutting the specimen;
- to stick specimen to the graphite specimen pedestal;

Though I've made the primary morphological and chemical analysis
and I
haven't observed great differences between the diferent automotive
paints
trades (while polyuretane paints and belayer metalizer paints already
tested),
but I'd found great differences between layers and layers of the paints
specimen.
As I'm not quite satisfied with the results obtained to be used
for
forensic purposes, I would greatly appreciate making contacts with
specialist
fo this subject, so as to receive any comments on this subject.
Thank you in advance for your help.
Jorgelina Altamirano
CERIDE,
Guemes 3450
(3000) Santa Fe, Argentina
e-mail: csedax-at-arcride.edu.ar



From daemon Tue Feb 08 16:21:10 2000



From: mauty-at-MOOREPARK.TEAGASC.IE
Date: Tue, 08 Feb 2000 11:22:33 +0000
Subject: HeNe laser

Contents Retrieved from Microscopy Listserver Archives
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Jorgelina
SEM/EDS//EPMA is only one class of analytical methods that the Forensic
community applies to paint analysis for comparison and association with
original source. Although used routinely, probe methods are not used
exclusively. The organic components in paints are generally more
discriminating.
Sample preparation usually entails some form of embedment and either
microtomy or polishing in order to reveal internal structure.
Removal of paint from an auto body is tricky, and requires practice.The
manufacturers have designed their paint systems to prevent removal!
I would be glad to provide you with additional resources.

Dennis

_________________________________________________________________________
Dennis C . Ward voice: 202-324-2982
FBI Laboratory fax: 202-324-4018
Microanalysis Laboratory email:
DCWard-at-concentric.net


----- Original Message -----
} From: {"csedax-at-alpha.arcride.edu.ar"-at-sparc5.Microscopy.Com}
To: {MICROSCOPY-at-sparc5.Microscopy.Com}
Sent: Tuesday, February 08, 2000 9:35 AM


Jorgelina
SEM/EDS//EPMA is only one class of analytical methods that the Forensic
community applies to paint analysis for comparison and association with
original source. Although used routinely, probe methods are not used
exclusively. The organic components in paints are generally more
discriminating.
Sample preparation usually entails some form of embedment and either
microtomy or polishing in order to reveal internal structure.
Removal of paint from an auto body is tricky, and requires practice.The
manufacturers have designed their paint systems to prevent removal!
I would be glad to provide you with additional resources.

Dennis

_________________________________________________________________________
Dennis C . Ward voice: 202-324-2982
FBI Laboratory fax: 202-324-4018
Microanalysis Laboratory email:
DCWard-at-concentric.net




----- Original Message -----
} From: "Bright, Alan" {ABright-at-brightinstruments.com}
To: {"csedax-at-alpha.arcride.edu.ar"-at-sparc5.Microscopy.Com} ;
{MICROSCOPY-at-sparc5.Microscopy.Com}
Sent: Tuesday, February 08, 2000 12:59 AM


Jorgelina
SEM/EDS//EPMA is only one class of analytical methods that the Forensic
community applies to paint for characterization and association with
original source. Although used routinely, probe methods are not used
exclusively. The organic components in paints are generally more
discriminating.
Sample preparation usually entails some form of embedment and either
microtomy or polishing in order to reveal layer structure.
Removal of paint from an auto body is tricky, and requires practice.The
manufacturers have designed their paint systems to prevent removal!
I would be glad to provide you with additional resources.

Dennis

_________________________________________________________________________
Dennis C . Ward voice: 202-324-2982
FBI Laboratory fax: 202-324-4018
Microanalysis Laboratory email:
DCWard-at-concentric.net




----- Original Message -----
} From: {"csedax-at-alpha.arcride.edu.ar"-at-sparc5.Microscopy.Com}
To: {MICROSCOPY-at-sparc5.Microscopy.Com}
Sent: Tuesday, February 08, 2000 9:35 AM


Dear all

I am planning to replace my Kr/Ar laser on a Zeiss 310 CSLM with two
separate lasers: 488nm Ar ion and HeNe 543nm. Does anyone have
experience of using a HeNe 543? I need this or something similar to
excite Rhodamine for food structure applications. I'd appreciate any
advice.

The reason I'm replacing the mixed gas laser is that has
averaged 25% downtime over the past three years.

Mark

Mark Auty
Dairy Products Research Centre
Moorepark, Fermoy, Co. Cork, Ireland.
mauty-at-moorepark.teagasc.ie
tel 00353 2542447
fax 00353 2542340




From daemon Tue Feb 08 16:21:36 2000



From: Stan Rice :      srice-at-alpha.utampa.edu
Date: Tue, 08 Feb 2000 09:04:43 -0400
Subject: LKB Nova

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear all,

Thank you for the many responses to my inquery about operation
instructions for the LKB Nova ultramicrotome. For future reference, I
have the instrument and the original instructions for the LKB Reichert
ultramicrotome if anyone has acquired one of these without instructions.
Thanks again for all of your responses.

Stan Rice
University of Tampa



From daemon Tue Feb 08 16:21:25 2000



From: Cap'n Peachfuzz :      bsteffen-at-arches.uga.edu
Date: Tue, 8 Feb 2000 08:42:57 -0500 (Eastern Standard Time)
Subject: Re: tungsten filaments

Contents Retrieved from Microscopy Listserver Archives
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On Mon, 7 Feb 2000 12:33:38 -0500 Marisa Ahmad
{mahmad-at-semiconductor.com} wrote:

} We are experiencing a lot of problems with the tungsten filaments that
} we are currently using on our JEOL 5800 SEM. During long acquisitions
} (many hours) there is a lot of brightness drift and shifting, even when
} lengthy stabilization periods are given prior to the acquisitions.
} JEOL has checked out the SEM itself, and can't find the source for the
} drifting

Marisa,

You can easily enough to see if it is filament drift. First, start the
filament alignment program to ensure alignment. Then with the beam on,
wait a few minutes for the filament to drift, then switch off the
autobeam function, and manually check the alignment using the 2
filament alignment knobs. If you can make the image brighter, then
there is filament drift. With my experiences with the 5800 though, I
suspect this is not the case.


W. L. (Buddy) Steffens, Ph.D
Dept. of Pathology
University of Georgia




From daemon Tue Feb 08 16:21:28 2000



From: Cap'n Peachfuzz :      bsteffen-at-arches.uga.edu
Date: Tue, 8 Feb 2000 09:02:01 -0500 (Eastern Standard Time)
Subject: Re: tungsten filaments

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



On Mon, 7 Feb 2000 16:35:56 -0500 (EST) William Tivol
{tivol-at-wadsworth.org} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}

} Dear Marisa, Do you have control over the filament voltage? If so,
} set the voltage just a hair over that necessary to saturate the
} filament. If the voltage is too low, parts of the filament will not
} emit electrons which get through the wehnelt and into the beam; if too
} high, there may be significant evaporation of the filament; either may
} cause drifting and/or shifting. I have had very good luck with the
} stabilities of both Agar and Ebtec (now Energy Beam Sciences)
} filaments; not that others may not be as good, I haven't tried them.
} Ebtec makes several shapes of filament, and one may be best for
} long-term stability. I have no interest in Agar or Ebtec except as a
} satisfied customer. Yours,
} Bill Tivol


Marisa,

This is a good point that Bill makes...if you are slightly
undersaturated, there will be fluctuations in the rate of electron
emission from the filament. The 5800 automatically aligns and
saturates the filament when the Autobeam mode is selected...and on our
5800, saturation in this mode is a bit low. If you turn off the
autobeam switch and use the filament emission knob to slightly increase
the emission current, this might solve the problem at a cost of only a
small decrease in filament life.



W. L. (Buddy) Steffens, Ph.D
Dept. of Pathology
University of Georgia



From daemon Tue Feb 08 16:21:46 2000



From: Huggins, Bradley J :      HUGGINBJ-at-BP.com
Date: Tue, 8 Feb 2000 09:11:56 -0500
Subject: Re: Computer lock-ups from static

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



John Hunt wrote:
}
} Dear Probe community,
} It is winter in Central NY, and the room humidity is 33%. Static
} electricity (I believe) has been causing frustrating computer lockups
} requiring rebooting our microprobe workstation.
} I am wondering if anyone has found the best solution for preventing
these
} problems.
}
John we had this same problem for many years while we were using the old
RT-11 based Tracor Northern and Kevex x-ray analyzers. The problem was
especially bad with the TN-5500. This was due to the keyboard with built in
scroll knob. The motion of turning that knob on the keyboard was perfect
for generating a little static. (This is pre- GUI and mouse days, except
for the Mac users!) Because of the low humidity conditions and severe
static accumulation, in winter months we were forced to set the entire
keyboard on a grounded pad. We also attached an antistatic strip to the
front of the keyboard. Because of the nature of the job, and the movement
to and from the computer and the microscope controls, we could not wear the
wrist straps. But with the conductive grounding strip across the entire
front of the keyboard we found that we could very easily get into the habit
of grounding our wrist or thumb on the strip before touching the rest of the
computer or keyboard. Then as we used the keyboard we would make some
effort to touch the grounding strip from time to time. This virtually
eliminated the problem. By the way I believe it was such a problem that
Tracor engineered a static discharge switch into the keyboard connection.


Brad Huggins
BPAmoco,
Naperville, Microscopy and Microanalysis



From daemon Tue Feb 08 16:21:58 2000



From: Leona Cohen-Gould :      lcgould-at-mail.med.cornell.edu
Date: Tue, 8 Feb 2000 12:44:34 -0400
Subject: Re: Diameter of human RBC?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 9:20 AM -0600 2/7/0, Michael Herron wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Confocal Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175




From daemon Mon Feb 07 17:12:19 2000



From: csedax-at-alpha.arcride.edu.ar
Date: Mon, 7 Feb 2000 17:36:17 -2359
Subject: SEM: automotive paints

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi!
My name is Jorgelina C. Altamirano. I'm an undegrated student of the
licenciate in chemical sciences.
I'm currently preparing my thesis, being its subjet: The morphological
characterization and the chemical analysis of automotive paints with forensic
purposes.
I'm undergoing my work using SEM and EPMA techniques with a JEOL,
JSM 35C and a microprobe EDAX PV9500.
I've chosen some papers as references:
- Beam et al, Analysis Protocol for discrimination for automotive
paints by SEM-EDAX, Journal of Forensic Sciences, 35, 5, 1990
- Zieba-Palus et al, Mikrochim. Acta, 14, 1997.

I've taken same samples of automotive paints (such as Fiat, Peugeot,
Renault, Ford, Chevrolet, Volkswagen) corresponding to the years 1994-99.
I've found many difficulties while preparing them in:
- separating the paint from the body work of the car;
- cutting the specimen;
- to stick specimen to the graphite specimen pedestal;

Though I've made the primary morphological and chemical analysis and I
haven't observed great differences between the diferent automotive paints
trades (while polyuretane paints and belayer metalizer paints already tested),
but I'd found great differences between layers and layers of the paints
specimen.
As I'm not quite satisfied with the results obtained to be used for
forensic purposes, I would greatly appreciate making contacts with specialist
fo this subject, so as to receive any comments on this subject.
Thank you in advance for your help.
Jorgelina Altamirano
CERIDE,
Guemes 3450
(3000) Santa Fe, Argentina
e-mail: csedax-at-arcride.edu.ar



From daemon Tue Feb 08 16:22:08 2000



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Tue, 08 Feb 2000 09:49:38 -0800
Subject: Re: Be X-ray peaks -I don't think so

Contents Retrieved from Microscopy Listserver Archives
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Dear Scott,
I've seen a Be Ka x-ray peak in a Link advertisement and we have generated
one in the Quartz XOne applications lab with a really good light element
deteector. You will find the Be Ka1 peak listed at 0.110 keV in most x-ray
tables.
At 10:52 AM 2/7/00 -0500, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Tue Feb 08 16:21:53 2000



From: Christoph Bauer :      cbauer-at-midway.uchicago.edu
Date: Tue, 08 Feb 2000 09:56:35 -0800
Subject: Desmosomes

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we are looking at desmosomes in mouse epidermis. My question: From
looking at sections we got the impression that demosomes are rather
uniform, round knob-like structures. As we did not do any serial
sections, we are not sure if this is true. Does anybody know of a
publication dealing with this?

Thanks for your help,

Christoph



Christoph Bauer Ph.D.
University of Chicago
Molecular Genentics and Cell Biology
5841 S. Maryland Ave/MC 1028
Chicago, Il 60637



From daemon Tue Feb 08 16:22:09 2000



From: Ingber, Bruce F. :      bingber-at-commserver.srrc.usda.gov
Date: Tue, 8 Feb 2000 11:56:46 -0600
Subject: Re: FEI/Philips CM12 SAD focus

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I received the following fax from Ms. Irene Piscopo, EM Consultant,
regarding electron diffraction procedures for the CM-12 or CM-120 TEM's from
FEI/Philips. I'll copy the information for those interested.

ELECTRON DIFFRACTION PROCEDURES

There are three methods for doing electron diffraction in the CM-12
involving two different techniques: Method I is an aperture limiting method
while Methods II and III are probe limiting. In all cases, the sample must
be in the eucentric position and focused.

METHOD I: (area } 1 µm) SAD

1. Select the SA mode (the diffraction aperture and image are focused in the
same plane).
2. Insert and center the SA aperture around the area of interest.*
3. Select D.
4. Remove the objective aperture.
5. Overfocus the second condenser to sharpen the pattern (i.e. parallel
illumination conditions).
6. Focus the pattern with the focus control which is now changing the
diffraction lens).
7. Change the size of the pattern (i.e. camera length) by changing the
magnification.

Size of the SA aperture
* Size of area selected =
----------------------------------------------------------------------------
------------
Objective lens magnification in D aperture
plane (~ 30x**)

** Actual magnification is focal length dependent.

METHOD II: (areas down to 40 nm with w filament; 20 nm with LaB6)

1. Locate an area of interest.
2. Remove the objective aperture.
3. Using Condensers 1 and 2, bring the beam down to a size slightly smaller
than the area of the area from which the diffraction pattern is to be
obtained.
4. Go to D and select the desired CL with the magnification control.
5. Focus the pattern with the focus control. Note: While it's sometimes
difficult to determine exact focus, it's often easier to insert an objective
aperture and focus the edge of the aperture to determine diffraction focus.
6. The size of the discs within the pattern can be decreased by decreasing
the size of the condenser aperture. Be sure the aperture is well-centered.

METHOD III: NANOPROBE and/or STEM (to 2 nm)

For diffraction and/or chemical information in the TEM mode from
regions of the sample less than 40 nm in diameter requires operating the
instrument in either the NANOPROBE or STEM modes.

NANOPROBE:

1. Depress NANOPROBE.
2. Follow instructions in Method II.

STEM:

1. Obtain a focused STEM image using the smallest C2 aperture.
2. Stop the scan.
3. Lower the main screen (CBED pattern)
4. Slightly larger probes and more parallel beam conditions can be obtained
using UUD and focusing with INTENSITY (C2 lens).

NOTES ON ELECTRON DIFFRACTION

1. The sample must always be eucentric and focused.
2. In METHOD I, the area from which the diffraction pattern is obtained is
determined by the selected area - (diffraction) aperture, when in SA mode.
To sharpen the ED pattern, one overfocuses the second condenser (C2)
3. In METHODS II and III, the area from which the diffraction pattern is
selected depends on the size of the beam. The size of the beam can be
altered by changing condenser lenses C1 and C2 (i.e. spot size and
intensity. To sharpen the ED pattern , one reduces the size of the C2
aperture.
4. Spherical aberration limits METHOD I to ~1 µm.
5. In METHODS II and III, spherical aberration of the objective lens is not
the limiting factor, the minimum probe size is.
6. For identifying an ED pattern, at least three rings are required.
7. Be sure the sample and the standard are in the eucentric position.
Changes in the objective current will cause variations in CL.


Bruce F. Ingber, Biologist
USDA-ARS, SRRC
1100 Robert E. Lee Blvd.
New Orleans, LA 70124

(504) 286-4270 phone
(504) 286-4419 fax
bingber-at-nola.srrc.usda.gov




From daemon Tue Feb 08 16:22:11 2000



From: Walck. Scott D. :      walck-at-ppg.com
Date: Tue, 8 Feb 2000 12:57:15 -0500
Subject: RE: Be X-ray peaks -I don't think so

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I'm wrong. I guess I should have looked up at the X-ray periodic table
above my head and saw the 0.108 keV value for Be. The reason that I was
wrong was that I did not consider the solid state aspects. I am still right
about not having a 2s1/2 to a 1s1/2 transition and that an X-ray carries
away 1h-bar of angular momentum. What I didn't do was think about the band
structure of a solid. If you have the solid, the 2p bands of Be most be
spilling into or be the conduction band for the electrons. That must be the
source for the electronic transition. I guess I should have engaged brain
before typing.
My most humble apologies.
-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)


"The opinions expressed are those of Scott D. Walck and not of PPG
Industries, Inc. nor of any PPG-associated companies."
--

} -----Original Message-----
} From: microprobe [mailto:chris.salter-at-materials.oxford.ac.uk]
} Sent: Tuesday, February 08, 2000 7:10 AM
} To: Walck. Scott D.
} Subject: Re: Be X-ray peaks -I don't think so
}
}
} Dear Walck,
}
} You may not, in theory, be able to produce Be X-rays, but we
} can detect them on our JEOL JXA 8800, they correspond to the position
} in the tables for Be Ka!, and the target is pure Be.
}
} On Mon, 7 Feb 2000 10:52:29 -0500 "Walck. Scott D." {walck-at-ppg.com}
} wrote:
}
} -----Original Message-----
} From: Ekstrom, Harry [mailto:harry.ekstrom-at-honeywell.com]
} Sent: Monday, February 07, 2000 7:40 PM
} To: 'Walck. Scott D.'
} Subject: RE: Be X-ray peaks -I don't think so
}
}
} I don't agree Scott. An L or M line for any element would
} not be located in
} the area
} of Be Ka. And the peak I normally get there with an ultra thin window
} is a well defined peak with great resolution.
} Please re evaluate your theory.
}
} Harry Ekstrom
} } }
} --------------------------------------------------------------
} ----------
} } The Microscopy ListServer -- Sponsor: The Microscopy
} Society of America
} } To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} } On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.} html
} }
}
} --------------------------------------------------------------
} ---------.
} }
} }
} } When a characteristic X-ray is given off from an atom, it
} carries 1 h-bar
} } (Planck's constant divided by 2 pi) of angular momentum.
} The electronic
} } transition that occurred for the X-ray to come off must
} conserve angular
} } momentum of the system. Therefore, a transition from a
} 2s1/2 to a 1s1/2
} } state is forbidden by selection rules. You can't produce a
} Be X-ray.
} } -Scott
} }
} } Scott D. Walck, Ph.D.
} } PPG Industries, Inc.
} } Glass Technology Center
} } Guys Run Rd. (packages)
} } P. O. Box 11472 (letters)
} } Pittsburgh, PA 15238-0472
} }
} } Walck-at-PPG.com
} }
} } (412) 820-8651 (office)
} } (412) 820-8161 (fax)
} }
} }
} }
}
} -------------------
} microprobe
} chris.salter-at-materials.ox.ac.uk
}
} * This e-mail message was sent with Execmail V5.0.x *
}



From daemon Wed Feb 09 23:52:18 2000



From: Steve Chapman :      PROTRAIN-at-CompuServe.COM
Date: Tue, 8 Feb 2000 15:31:08 -0500
Subject: Diffraction & Coherence

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Hi

It has been very interesting reading about diffraction and electron beams
but it is quite clear that we often give the instrument credit, "the
instrument does it automatically", when credit is not due. Almost every
"automatic" is a designers estimate or better still a calculation, it is a
setting!

After 36 years with TEM I have to say that the only area that the
instrument could set in the SA mode could be the approximate position of
the first image plane. We normally move to the SA mode to free up the
diffraction lens (1st intermediate) to enable it to be focussed on thefirst
image plane and the diffraction (intermediate) aperture, independent of the
magnification system. We would then focus the image inside the aperture
with the objective lens to ensure that the selected area IS the area in the
diffraction pattern. Without this procedure electron rotation and
misalignments could give you a diffraction pattern from an area not in the
centre of the screen! Users are correct in that if they set the eucentric
point or better still, adjust the specimen height to focus at the same
objective lens current value, that done the first image plane will be at
the same point within the diffraction (intermediate) lens.

So how could the Philips work? I would guess they set the diffraction lens
at approximately the first image plane. Then as they do not use it within
their lens scheme for the SA magnifications it is fixed at this focus, good
enough I think?

A second point, parallel beams are very important if you are chasing the
best image quality, biology or materials, most times we need high
coherence. There are misunderstandings on how to obtain a parallel beam or
high coherence, setting the final condenser underfocus is incorrect. The
procedure for high coherence would be to use the smallest spot size you can
tolerate (this probably means you must up the emission current). Once in
this condition overfocus the final condenser (clockwise from crossover) the
spot, what ever size it is now, becomes your virtual source. The further
overfocus you go the greater the beam coherence . You will deduce the
smaller the condenser aperture the sharper the spot and the smaller the
spot the greater the coherence for a given degree of overfocus.

Work with a design team and they expect everyone to overfocus, they expect
everyone to use small condenser apertures and they expect everyone to use
small spot sizes. They do not expect everyone to use too low an emission
current (almost everyone does, we have talked before about filament life
being the most important feature of many laboratories) because this makes
the task too difficult!


Steve Chapman
Senior Consultant
Protrain
Electron microscopy courses world wide
http://www.emcourses.com
Tel & Fax 44 (0) 1280 814774



From daemon Tue Feb 08 16:22:19 2000



From: Schibler, Matthew :      MSchibler-at-mednet.ucla.edu
Date: Tue, 8 Feb 2000 12:42:13 -0800
Subject: RE: HeNe laser

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} Dear all

} I am planning to replace my Kr/Ar laser on a Zeiss 310 CSLM with two
} separate lasers: 488nm Ar ion and HeNe 543nm. Does anyone have
} experience of using a HeNe 543? I need this or something similar to
} excite Rhodamine for food structure applications. I'd appreciate any
} advice.

} The reason I'm replacing the mixed gas laser is that has
} averaged 25% downtime over the past three years.

} Mark

} Mark Auty
} Dairy Products Research Centre
} Moorepark, Fermoy, Co. Cork, Ireland.
} mauty-at-moorepark.teagasc.ie
} tel 00353 2542447
} fax 00353 2542340


I have been using the Argon ion (488nm) and HeNe 543nm combination on
different Zeiss LSM310's and 410's for the past 9 years. The HeNe 543nm is
fairly low power, but it has been perfectly adequate for scanning the
fluorochromes I have needed to see including TRITC, Texas Red, Cy3,
propidium iodide, and Sirius Red. I have never had a problem with the laser
going down either, but one would expect that with a HeNe laser.

Slàinte,

Matthew J. Schibler Ph.D.
UCLA Brain Research Institute
1524A Gonda (Goldschmied) Center
for Neuroscience and Genetics
Los Angeles, CA 90095-1761

(310) 825-9783
FAX (310) 206-5855
E-mail: mschibler-at-mednet.ucla.edu



From daemon Tue Feb 08 16:22:25 2000



From: Kristen Lennon :      kalen-at-citrus.ucr.edu
Date: Tue, 08 Feb 2000 14:06:58 -0800
Subject: cryo chamber for LRWhite UV

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Hi All,
Does anyone have experience with the Pelco UVC2 or UVC1 Cryo Chamber for UV
curing of resins (especially LR White) or with something similar? We're
contemplating buying something like that for UV polymerization of LR White
at -20 C.
Thanks for your help,
Kristen
Kristen A. Lennon
Cell, Molecular & Developmental Biology Group
Department of Botany & Plant Sciences
University of California
Riverside, CA 92521
kalen-at-citrus.ucr.edu



From daemon Tue Feb 08 21:26:51 2000



From: Jim McGee :      mcgee-at-geol.sc.edu
Date: Tue, 08 Feb 2000 17:09:09 -0500
Subject: Re: Be X-ray peaks -I don't think so

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Scott,
I'm not sure what brought this up, but my reaction is "thems fightin'
words". I do not currently have a wavelength reflecting crystal to measure
that low in the periodic table, but I did on another instrument. I distinctly

recall seeing a pretty hefty peak at the Be K-alpha position when I focused
the beam down on a piece of pure Be metal. I guess rules are made to be
broken. Are your calculations correct? Or am I misunderstanding something
here?

Jim
--
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
James J. McGee (email: jmcgee-at-sc.edu)
Department of Geological Sciences
University of South Carolina
Columbia, SC 29208

Tel: 803-777-6300 Fax: 803-777

"Walck. Scott D." wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} When a characteristic X-ray is given off from an atom, it carries 1 h-bar
} (Planck's constant divided by 2 pi) of angular momentum. The electronic
} transition that occurred for the X-ray to come off must conserve angular
} momentum of the system. Therefore, a transition from a 2s1/2 to a 1s1/2
} state is forbidden by selection rules. You can't produce a Be X-ray.
} -Scott
}
} Scott D. Walck, Ph.D.
} PPG Industries, Inc.
} Glass Technology Center
} Guys Run Rd. (packages)
} P. O. Box 11472 (letters)
} Pittsburgh, PA 15238-0472
}
} Walck-at-PPG.com
}
} (412) 820-8651 (office)
} (412) 820-8161 (fax)



From daemon Tue Feb 08 21:26:57 2000



From: Rosemary Walsh :      rw9-at-psu.edu
Date: Tue, 8 Feb 2000 16:24:35 -0600
Subject: Size determination of overlapping particles

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Dear Listers,
I have a student interested in sizing ice crystals
which overlap. Any suggestions?
Rosemary




From daemon Tue Feb 08 21:27:04 2000



From: Rosemary Walsh :      rw9-at-psu.edu
Date: Tue, 8 Feb 2000 16:24:35 -0600
Subject: Size determination of overlapping particles

Contents Retrieved from Microscopy Listserver Archives
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Dear Listers,
I have a student interested in sizing ice crystals
which overlap. Any suggestions?
Rosemary




From daemon Tue Feb 08 21:27:07 2000



From: Rosemary Walsh :      rw9-at-psu.edu
Date: Tue, 8 Feb 2000 16:24:35 -0600
Subject: Size determination of overlapping particles

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers,
I have a student interested in sizing ice crystals
which overlap. Any suggestions?
Rosemary




From daemon Tue Feb 08 21:27:08 2000



From: Rosemary Walsh :      rw9-at-psu.edu
Date: Tue, 8 Feb 2000 16:24:35 -0600
Subject: Size determination of overlapping particles

Contents Retrieved from Microscopy Listserver Archives
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Dear Listers,
I have a student interested in sizing ice crystals
which overlap. Any suggestions?
Rosemary




From daemon Tue Feb 08 21:27:10 2000



From: Rosemary Walsh :      rw9-at-psu.edu
Date: Tue, 8 Feb 2000 16:24:35 -0600
Subject: Size determination of overlapping particles

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers,
I have a student interested in sizing ice crystals
which overlap. Any suggestions?
Rosemary




From daemon Tue Feb 08 21:27:17 2000



From: Rosemary Walsh :      rw9-at-psu.edu
Date: Tue, 8 Feb 2000 16:24:35 -0600
Subject: Size determination of overlapping particles

Contents Retrieved from Microscopy Listserver Archives
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Dear Listers,
I have a student interested in sizing ice crystals
which overlap. Any suggestions?
Rosemary




From daemon Wed Feb 09 16:48:39 2000



From: Rosemary Walsh :      rw9-at-psu.edu
Date: Tue, 8 Feb 2000 16:24:35 -0600
Subject: Size determination of overlapping particles

Contents Retrieved from Microscopy Listserver Archives
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Dear Listers,
I have a student interested in sizing ice crystals
which overlap. Any suggestions?
Rosemary




From daemon Wed Feb 09 16:48:49 2000



From: Rosemary Walsh :      rw9-at-psu.edu
Date: Tue, 8 Feb 2000 16:24:35 -0600
Subject: Size determination of overlapping particles

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers,
I have a student interested in sizing ice crystals
which overlap. Any suggestions?
Rosemary




From daemon Wed Feb 09 16:48:51 2000



From: Rosemary Walsh :      rw9-at-psu.edu
Date: Tue, 8 Feb 2000 16:24:35 -0600
Subject: Size determination of overlapping particles

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers,
I have a student interested in sizing ice crystals
which overlap. Any suggestions?
Rosemary




From daemon Wed Feb 09 16:48:57 2000



From: Rosemary Walsh :      rw9-at-psu.edu
Date: Tue, 8 Feb 2000 16:24:35 -0600
Subject: Size determination of overlapping particles

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers,
I have a student interested in sizing ice crystals
which overlap. Any suggestions?
Rosemary




From daemon Wed Feb 09 16:49:13 2000



From: Rosemary Walsh :      rw9-at-psu.edu
Date: Tue, 8 Feb 2000 16:24:35 -0600
Subject: Size determination of overlapping particles

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers,
I have a student interested in sizing ice crystals
which overlap. Any suggestions?
Rosemary




From daemon Wed Feb 09 23:52:17 2000



From: Rosemary Walsh :      rw9-at-psu.edu
Date: Tue, 8 Feb 2000 16:24:35 -0600
Subject: Size determination of overlapping particles

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers,
I have a student interested in sizing ice crystals
which overlap. Any suggestions?
Rosemary




From daemon Tue Feb 08 21:26:55 2000



From: Dr Eric Lachowski :      che136-at-abdn.ac.uk
Date: Tue, 8 Feb 2000 16:24:58 -0600
Subject: Be X-ray and filament drift

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Dear Listers,
I have responses to two of today's postings, but they are
quite unrelated.
1. I did not see the original question about Be x-rays,
only Scott Walk's reply, so I don't know what info was
being sought. We used a Be-containing mineral to evaluate
microprobes before buying. I won't embarrass those who
failed, but only one manufacturer succeeded. The reason
the others failed is probably that in compounds the x-ray
energy is subject to largish chemical shifts relative to
pure Be because there is no shielding by outer electrons
and you need to search on either side of the tabulated
value of about 0.1keV. So there IS a Be x-ray and a
reasonable WDS spectrometer should detect it.

2. Filament drift. Fred Schamber's reply is right most of
the time, but I did once have a batch of 6 JEOL-type
filaments made by an EM supply house where 2 of them
drifted until the tips were right at the edge of the hole
and stayed there even when the filament was cooled down
again. This was the only time in about 15 years of using
JEOL microscopes.

Eric
----------------------
Dr Eric Lachowski
Department of Chemistry
University of Aberdeen
Meston Walk
Old Aberdeen AB24 3UE
Scotland
+44 (0)1224 272934 fax 272921
e.lachowski-at-abdn.ac.uk




From daemon Tue Feb 08 21:26:54 2000



From: Dean Armytage :      hillstream-at-hypermax.net.au
Date: Tue, 8 Feb 2000 16:27:01 -0600
Subject: Live Blood Staining

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Hi List I am using live blood staining light microscopy, is there
anyone who has tried or is using this technique. I would like to swap
notes. Dean Armytage PhD Hillstream Health Centre Australia




From daemon Tue Feb 08 21:27:20 2000



From: Ekstrom, Harry :      harry.ekstrom-at-honeywell.com
Date: Tue, 8 Feb 2000 18:55:31 -0700
Subject: Re: Be X-ray peaks -I don't think so

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I'm getting more curious as I read on here. Speaking strictly energy now
and leaving out wavelength, the characteristic energy of Be Ka is about .11
KeV like Mary stated. I've had an occasional peak show up there before and
thought it may have been Be. Now I understand that it may have been "noise"
all along. Would "noise" be a pretty defined and relatively well resolved
peak at that energy level using an ultra thin window? Depending on the low
energy cutoff setting, one might truly have Be and never detect it. Would
lowering the KV reduce the noise artifact?

I guess I'll get out my Be planchet and try a few things.

Harry

-----Original Message-----
} From: Jim McGee [mailto:mcgee-at-geol.sc.edu]
Sent: Tuesday, February 08, 2000 3:09 PM
To: 'Microscopy'


Scott,
I'm not sure what brought this up, but my reaction is "thems fightin'
words". I do not currently have a wavelength reflecting crystal to measure
that low in the periodic table, but I did on another instrument. I
distinctly

recall seeing a pretty hefty peak at the Be K-alpha position when I focused
the beam down on a piece of pure Be metal. I guess rules are made to be
broken. Are your calculations correct? Or am I misunderstanding something
here?

Jim
--
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
James J. McGee (email: jmcgee-at-sc.edu)
Department of Geological Sciences
University of South Carolina
Columbia, SC 29208

Tel: 803-777-6300 Fax: 803-777

"Walck. Scott D." wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} When a characteristic X-ray is given off from an atom, it carries 1 h-bar
} (Planck's constant divided by 2 pi) of angular momentum. The electronic
} transition that occurred for the X-ray to come off must conserve angular
} momentum of the system. Therefore, a transition from a 2s1/2 to a 1s1/2
} state is forbidden by selection rules. You can't produce a Be X-ray.
} -Scott
}
} Scott D. Walck, Ph.D.
} PPG Industries, Inc.
} Glass Technology Center
} Guys Run Rd. (packages)
} P. O. Box 11472 (letters)
} Pittsburgh, PA 15238-0472
}
} Walck-at-PPG.com
}
} (412) 820-8651 (office)
} (412) 820-8161 (fax)



From daemon Wed Feb 09 23:52:21 2000



From: Hong Yi :      hyi-at-emory.edu
Date: Tue, 8 Feb 2000 21:26:04 -0500 (EST)
Subject: Re: New Gold Enhancement Reagents

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Dear Michael:

My personal experience with silver enhancement dates back to when Janssen
Life Sciences first introduced the IntenSE M silver enhancement kit in
late 80's (this product was later taken over by Amersham). At the time, I
felt the IntenSE M was easy to use, but its fast reaction made it hard to
control the particle growth. Just like you, the desire for a better
results has kept me searching and testing whenever a new protocol or
product became available.

Danscher's method gives wonderful intensification, but its low pH
sometimes damages the ultrastructure when used for the pre-embedding
immunogold labeling. Burry's method and the Nanoprobes HQ silver made
progress with pH, however they inherited Danscher's high viscosity and
light sensitivity, which limit their penetration in pre-embedding silver
enhancement and render it more difficult to handle from a practical
standpoint. I have also tested the Nanoprobes GoldEnhance kit for
pre-embedding immunogold labeling recently. At the LM level the results
were decent. I have not spent a lot of time evaluating its performance
at the EM-level. So far, the particle size homogeneity is not as good as
what I've found with Danscher's method. But I will refrain from further
judgment until more work has been done.

In my opinion, the first breakthrough in intensifying gold particles since
Danscher's method was realized when Aurion, a Dutch company, first
introduced the R-gent SE-EM silver enhancement kit last June. As you
wished for in your post, it has a near-neutral pH, low viscosity, and it
is light insensitive. Moreover, it gives a great enhancement efficiency
and even particle size and shape. Background remains minimal even after
two hours enhancement on the bench. Morphology preservation after
enhancement is superior to any other enhancement reagent I have used. I
have been testing this kit profusely for both post- and pre-embedding
immunogold labeling on various types of samples (including cell cultures
and vibratome sections), with many different primary and secondary
antibodies, and am very pleased with its performance. I use 0.5% OsO4 for
20 min and have never had any problem with silver disappearing. If you
like, I would be very happy to e-mail some images to you so you can
evaluate them yourself.

Hong
=================
Hong Yi
Emory University
Department of Neurology
6215 Woodruff Memorial Research Building
1639 Pierce Dr.
Atlanta, GA 30341
Phone: (404) 727-8692
Fax: (404) 727-3157
Email: hyi-at-emory.edu

On Thu, 3 Feb 2000, Michael Plociniak wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear Colleagues,
} Has anyone used gold enhancement reagents from Nanoprobes, Inc.
} (Stonybrook, NY - USA) as an alternative to silver enhancement for
} enlarging 1 nm immunogold probes?
}
} Advertisements state that gold enhancement has lower background and is
} compatible with osmium. Can anyone verify this from personal experience?
}
} In addition to these advantages, I am hoping that milder pH conditions will
} be less damaging to ultrastructure in cultured neurons (using a
} preembedment protocol).
}
} Thank you,
} Michael
}





From daemon Wed Feb 09 23:52:29 2000



From: Radostin Danev :      rado-at-nips.ac.jp
Date: Wed, 9 Feb 2000 12:01:47 +0900
Subject: Finding the diffraction plane

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi everybody,

Here is the way I found yesterday for finding the diffraction plane. By
diffraction plane I mean the plane at which diffraction pattern is formed
(crossover, Fourier of the object wave) not the theoretical BFP of the
objective.

First perform standard alignment for brightfield mode. Then go to 10 000X.
There are several ways to perform:

1. Fixed illumination and specimen position (i.e. OL current)

a) Adjust the desired brightness with C2. Adjust the desired OL current and
specimen position.
b) Insert OL aperture and move it so that the edge of the aperture to cross
through the middle of the screen. If the aperture is exactly at the diff.
plane then you'll not be able to see its edge - just the whole beam will
fade uniformly ... so you have the diff. plane.
c) If you see aperture edge then it is not at diff. plane. Now two ways:
- change the aperture z-position (not recommended ... but if it is very far
..)
- If your microscope has condenser minilens you can try tweaking both C2 and
CML in order to keep the intensity same and just make the aperture edge
disappear (i.e. the whole screen uniformly darkened).

2. Fixed specimen (i.e. OL current).

a) Focus the specimen
b) same as 1b
c) Change C2 excitement in order to make the edge disappear

3. Fixed illumination

a) Set desired C2 excitement
b) same as 1b
c) Change the objective lens current until the aperture edge disappears.
Then move specimen in z-direction until it is focused.


In all these methods after adjusting the aperture at diff. plane you can try
tweaking C2 or OL ... you will see that the aperture is not at diff. plane
anymore, The edge will appear on one or the other side (i.e. mirrored)
depending on the direction of the lens current change.
Another thing - after adjusting the aperture at diff. plane you can check if
beam shift tilts the beam (by tilting here I mean - if the specimen is
illuminated with plane wave then shifting the beam should not tilt the wave
front). Shift the beam and if the brightness changes (when the aperture edge
partially covers the zero diff. spot) then the beam is tilting.
I wish there was a IL1 tweaking knob allowing for change of the IL1 current
while in brightfield mode. This wold make the things much more easier.

About the methods for making the illumination at the specimen parallel. They
will work only if:
- The OL aperture is positioned by the manufacturer exactly at the
theoretical BFP (I think that in most of the TEMs it is not)
- The OL current should be set to zero deviation (i.e. at the value for
which the BFP position was calculated)
- The intermediate lens system should be properly calibrated by the
manufacturer so that if both of the above conditions are satisfied and the
specimen is at front focal plane of the OL then it to be in focus. In other
words the theoretical BFP of the objective to coincide with the theoretical
front focal plane of the IL1.

I don't think parallel illumination is so very important ... using spherical
wave will give the same results but just the diff. planes will be shifted.

All of the above is just my opinion. I could be mistaking somewhere ...

Best wishes,

Rado

---------------------------------------------------------------------
Radostin Danev
Laboratory of Ultrastructure Research
National Institute for Physiological Sciences
Myodaiji-cho, Okazaki 444-8585, JAPAN
e-mail: rado-at-nips.ac.jp
---------------------------------------------------------------------



From daemon Wed Feb 09 16:48:37 2000



From: Gordon Couger :      gcouger-at-couger.com
Date: Tue, 8 Feb 2000 21:31:38 -0600
Subject: Re: automotive paints

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



----- Original Message -----
} From: "Dennis Ward" {DCWard-at-concentric.net}
}
} Jorgelina
} SEM/EDS//EPMA is only one class of analytical methods that the
Forensic
} community applies to paint analysis for comparison and association with
} original source. Although used routinely, probe methods are not used
} exclusively. The organic components in paints are generally more
} discriminating.
} Sample preparation usually entails some form of embedment and either
} microtomy or polishing in order to reveal internal structure.
} Removal of paint from an auto body is tricky, and requires
practice.The
} manufacturers have designed their paint systems to prevent removal!
} I would be glad to provide you with additional resources.

I have never do this but this is what i would try first.

One thing I would try on would be removing the metal from the paint. A
weak elecrolite solution and a low DC voltage connected to reverse
electoplate the metal away sould get rid of almost all of it and the last
bit could be removed with acid or evaporated as the cathode in a vacum
chamber.

Once you get the metal down to a bunch of small islands you might be able
to let it rust free of the paint.

I have no idea what this would do to the paint but most paint films I have
delt with are pretty tough.

Gordon

Gordon Couger gcouger-at-couger.com

Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00




From daemon Wed Feb 09 16:48:56 2000



From: Massimo Catalano :      massimo.catalano-at-ime.le.cnr.it
Date: Wed, 09 Feb 2000 09:43:03 +0100
Subject: PIPS and milling damage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listservers,

we are massively working on analytical and structural TEM characterization
of semiconductors, mainly InGaAs/GaAs, quantum well, wires and dots.

Specimen preparation is of course a crucial point of our work.

We have just switched to PIPS (we used to make our samples using the Gatan
Duo Mill), and we are getting controversial results about the damage
introduced by the milling procedure.

We are perfectly aware of all the differences between the two instruments,
especially the absence of cooling stage and the higher beam current.

Has anyone performed a systematic and careful analysis to try to evaluate
the milling damage on similar materials systems. We are willing to start a
systematic work to assess this issue, but would like to know if anyone has
already done anything on this topic. Also, any collaboration will be more
than welcome.

Thanks.

Massimo




Dr. Massimo Catalano
CNR-IME
Campus Universitario
Via Arnesano
73100 Lecce - ITALY
tel: + 39 0832 322362
fax: + 39 0832 325299
email: massimo.catalano-at-ime.le.cnr.it
http://www.ime.le.cnr.it
http://www.ime.le.cnr.it/sime/sime.htm




From daemon Wed Feb 09 23:52:27 2000



From: flaitz-at-us.ibm.com
Date: Wed, 9 Feb 2000 07:48:09 -0500
Subject: PIPS and milling damage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Dear Massimo,

in our lab we have prepared a number of GaAs, AlGaAs, InGaAs devices.
Mechanical thinning is by the tripod wedge technique, but typically we end
up with more damage than we find for Si samples so there is a need to leave
samples thicker and use more extensive ion milling for final thinning.

We also have a PIPS and I have found its capabilities essential for
achieving good thin samples in these materials. I found more success by
using low angles and low KeV for long times. Typically I would be using
5-6 degrees and 4-4.5 KeV initially, with 3-4 degrees and 3-3.5 KeV for the
final ion polishing. On samples 2-3 microns thick, this would amount to
2-3 hours total milling time. I did not observe any artefacts introduced
with milling under these conditions, and the junction structures and
inherent defects were clearly observed.

The conditions above should work with any ion miller capable of low angle
milling, but our experience is only with the PIPS. If you use the wedge
technique for mechanical thinning, I would recommend that you use Mo grids
to mount your specimens as the extended milling times at low angles will
really chew up a Cu grid. Also, Mo grids are considerably thinner,
allowing angles as low as 3-4 degrees on the grid hole side for a sample
positioned in the middle of a 1 mm grid hole.

If you would like, I can send you some typical images of devices we have
prepared, including images showing the removal of the mechanical polishing
damage.


Philip L. Flaitz
IBM Analytical Services, Hopewell Junction, NY
http://www.chips.ibm.com/services/asg
Ph.......(914) 892-3094, FAX -2003
flaitz-at-us.ibm.com



Massimo Catalano {massimo.catalano-at-ime.le.cnr.it} on 02/09/2000 03:43:03 AM

To: Microscopy-at-sparc5.Microscopy.Com
cc:


Dear Listservers,

we are massively working on analytical and structural TEM characterization
of semiconductors, mainly InGaAs/GaAs, quantum well, wires and dots.

Specimen preparation is of course a crucial point of our work.

We have just switched to PIPS (we used to make our samples using the Gatan
Duo Mill), and we are getting controversial results about the damage
introduced by the milling procedure.

We are perfectly aware of all the differences between the two instruments,
especially the absence of cooling stage and the higher beam current.

Has anyone performed a systematic and careful analysis to try to evaluate
the milling damage on similar materials systems. We are willing to start a
systematic work to assess this issue, but would like to know if anyone has
already done anything on this topic. Also, any collaboration will be more
than welcome.

Thanks.

Massimo




Dr. Massimo Catalano
CNR-IME
Campus Universitario
Via Arnesano
73100 Lecce - ITALY
tel: + 39 0832 322362
fax: + 39 0832 325299
email: massimo.catalano-at-ime.le.cnr.it
http://www.ime.le.cnr.it
http://www.ime.le.cnr.it/sime/sime.htm








From daemon Wed Feb 09 16:49:23 2000



From: LAB. DE MICROSCOPIA ELECTRONICA - FI - UNER :      microsc-at-fi.uner.edu.ar
Date: Wed, 9 Feb 2000 13:10:00 +0000
Subject: looking for ETEC Company

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello .....
we have an old ETEC Omniscan SEM, and we are searching for ETEC company or any
people that can supply manuals ....

any help is welcome

best regards


===================================================
Fernando D. Balducci
Laboratorio de Microscopia Electr—nica
Facultad de Ingenier’a - Bioingenieria
Universidad Nacional de Entre Rios
Argentina
e-mail: microsc-at-fi.uner.edu.ar
tel: 54 43 975100
fax: 54 43 975077


===================================================



From daemon Wed Feb 09 16:49:27 2000



From: Corazon Bucana :      bucana-at-audumla.mdacc.tmc.edu
Date: Wed, 09 Feb 2000 08:11:32 -0600
Subject: autofluorescence quenching

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am working with archival specimens of formalin-fixed paraffin embedded
tissues and I am getting tremendous autofluorescence. I looked at tissue
sections after the sections have been de-waxed using either FITC or
rhodamine filter sets and red cells and connective tissues are brightly
fluorescent. Is there a way of suppressing the autofluorescence and still
retain reactivity of tissue to antibodies? I would appreciate any comments
or suggestions.

*******************************************************
Corazon D. Bucana, Ph.D.
Department of Cancer Biology
U.T. M.D. Anderson Cancer Center
1515 Holcombe Blvd. Box 173
Houston, Texas 77030
Phone: (713) 792-8106
FAX: (713) 792-8747
Email:bucana-at-audumla.mdacc.tmc.edu
FAX: (713) 792-8747



From daemon Wed Feb 09 16:49:25 2000



From: Jim McGee :      mcgee-at-geol.sc.edu
Date: Wed, 09 Feb 2000 10:26:27 -0500
Subject: Re: Be X-ray peaks -I don't think so

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Harry,

I don't have a UTW detector here, so I'll leave this to the EDS experts. But,
on the JEOL 8800/8900 I used to run we had a thin window detector that could see
B (not Be) if the low end noise peak (which is huge) was properly
discriminated. Get out your C planchett while you are getting the Be one, and
see if you get the noise peak at Be. Far better to use wavelength than EDS down
at that end.

Jim McGee
--
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
James J. McGee (email: jmcgee-at-sc.edu)
Department of Geological Sciences
University of South Carolina
Columbia, SC 29208

Tel: 803-777-6300 Fax: 803-777-6610

"Ekstrom, Harry" wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} I'm getting more curious as I read on here. Speaking strictly energy now
} and leaving out wavelength, the characteristic energy of Be Ka is about .11
} KeV like Mary stated. I've had an occasional peak show up there before and
} thought it may have been Be. Now I understand that it may have been "noise"
} all along. Would "noise" be a pretty defined and relatively well resolved
} peak at that energy level using an ultra thin window? Depending on the low
} energy cutoff setting, one might truly have Be and never detect it. Would
} lowering the KV reduce the noise artifact?
}
} I guess I'll get out my Be planchet and try a few things.
}
} Harry
}
} -----Original Message-----
} } From: Jim McGee [mailto:mcgee-at-geol.sc.edu]
} Sent: Tuesday, February 08, 2000 3:09 PM
} To: 'Microscopy'
} Subject: Re: Be X-ray peaks -I don't think so
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Scott,
} I'm not sure what brought this up, but my reaction is "thems fightin'
} words". I do not currently have a wavelength reflecting crystal to measure
} that low in the periodic table, but I did on another instrument. I
} distinctly
}
} recall seeing a pretty hefty peak at the Be K-alpha position when I focused
} the beam down on a piece of pure Be metal. I guess rules are made to be
} broken. Are your calculations correct? Or am I misunderstanding something
} here?
}
} Jim
} --
} ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
} James J. McGee (email: jmcgee-at-sc.edu)
} Department of Geological Sciences
} University of South Carolina
} Columbia, SC 29208
}
} Tel: 803-777-6300 Fax: 803-777
}
} "Walck. Scott D." wrote:
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} } When a characteristic X-ray is given off from an atom, it carries 1 h-bar
} } (Planck's constant divided by 2 pi) of angular momentum. The electronic
} } transition that occurred for the X-ray to come off must conserve angular
} } momentum of the system. Therefore, a transition from a 2s1/2 to a 1s1/2
} } state is forbidden by selection rules. You can't produce a Be X-ray.
} } -Scott
} }
} } Scott D. Walck, Ph.D.
} } PPG Industries, Inc.
} } Glass Technology Center
} } Guys Run Rd. (packages)
} } P. O. Box 11472 (letters)
} } Pittsburgh, PA 15238-0472
} }
} } Walck-at-PPG.com
} }
} } (412) 820-8651 (office)
} } (412) 820-8161 (fax)

--
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
James J. McGee (email: jmcgee-at-sc.edu)
Department of Geological Sciences
University of South Carolina
Columbia, SC 29208

Tel: 803-777-6300 Fax: 803-777-6610




From daemon Wed Feb 09 16:49:30 2000



From: ipaul-at-MtRoyal.AB.CA
Date: Wed, 09 Feb 2000 09:26:15 -0700
Subject: Sarcomere cross-sections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am looking for TEM images that show cross-sectional views of skeletal muscle
sarcomeres during the *contracted state*. In particular, I am interested in
seeing the spacing of the thin filaments when they **overlap each other** (i.e.,
when those from one side of the sarcomere overlap those from the other side of
the sarcomere in the contracted state). In addition, I am interested in seeing
the distribution of the thin filaments as they pass through the M line. It
would be greatly appreciated if anyone could tell me if they know of any
research papers or review articles that contain such TEM images.

Thank you!

Izak Paul
Biological Sciences
Mount Royal College




From daemon Wed Feb 09 23:51:48 2000



From: David Knecht :      knecht-at-uconn.edu
Date: Wed, 9 Feb 2000 13:03:36 -0500
Subject: Paultek camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does anyone know if Paultek Imaging still exists and a phone number for
them (or Email)? Thanks-Dave


************************************************************

Dr. David Knecht
Department of Molecular and Cell Biology
University of Connecticut
75 N. Eagleville Rd. U-125
Storrs, CT 06269
Knecht-at-uconnvm.uconn.edu
860-486-2200 860-486-4331 (fax)
home page: http://www.sp.uconn.edu/~mcbstaff/knecht/knecht.html
************************************************************




From daemon Wed Feb 09 16:49:32 2000



From: Roger Moretz :      rcmoretz-at-excite.com
Date: Wed, 9 Feb 2000 10:06:34 -0800 (PST)
Subject: Re: Desmosomes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Christoph:
I don't have any references at hand, but there is a whole literature on
desmosomes. A search on Medline (or PubMed on the internet) should provide
you with a good list of EM related publications on desmosomes--probably more
than you ever wanted to know! As an aside, I think there may be some books
that cover this as well, but since it is not an area of personal expertise
or current research interest, I am afraid they have all gone from my memory
banks.

Roger


On Tue, 08 Feb 2000 09:56:35 -0800, Christoph Bauer wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} we are looking at desmosomes in mouse epidermis. My question: From
} looking at sections we got the impression that demosomes are rather
} uniform, round knob-like structures. As we did not do any serial
} sections, we are not sure if this is true. Does anybody know of a
} publication dealing with this?
}
} Thanks for your help,
}
} Christoph
}
}
}
} Christoph Bauer Ph.D.
} University of Chicago
} Molecular Genentics and Cell Biology
} 5841 S. Maryland Ave/MC 1028
} Chicago, Il 60637
}


Roger C Moretz, Ph.D.





_______________________________________________________
Get 100% FREE Internet Access powered by Excite
Visit http://freeworld.excite.com



From daemon Wed Feb 09 16:49:20 2000



From: Mary Gail Engle :      mgengle-at-pop.uky.edu
Date: Wed, 09 Feb 2000 10:06:35 -0800
Subject: Re: Desmosomes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Christoph,
I have examined alot of mouse and pig skin at the TEM level and the
desmosomes and hemidesmosomes appear rather typical, that is, small, long
bridge-like structures between the cells.

Mary Gail Engle
Electron Microscopy & Imaging Facility
University of Kentucky

At 09:56 AM 2/8/00 -0800, Christoph Bauer wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Wed Feb 09 23:51:42 2000



From: Fagerland,Jane :      jane.a.fagerland-at-abbott.com
Date: Wed, 9 Feb 2000 16:45:16 -0600
Subject: Summer internships

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The Department of Microscopy and Microanalysis at Abbott Laboratories has two
summer internships available for 8-12 weeks this summer. One position is in
Biological Microscopy, and one is in Materials Analysis and Microscopy.
We're looking for students who are considering a career in microscopy,
especially students interested in the pharmaceutical industry.

Abbott Laboratories is a diversified healthcare company that produces
pharmaceuticals, diagnostic devices, and hospital products. The Microscopy
department analyzes samples related to all these functions. We use polarized
light, fluorescence, TEM, SEM, EDXS, confocal microscopy, and flow cytometry
to solve problems related to products, as well as to provide support for
pharmaceutical Discovery and Development basic research and drug safety.

Housing and a stipend are provided for the summer. We're located near Lake
Michigan and the Wisconsin state border, about an hour's drive or train-ride
north of Chicago. There's lots to see and do in the area, and there are
planned activities with other interns, as well. The Abbott Summer Internship
Program has been nationally recognized as one of the best in the country.

If you're interested, please send a resume to:

Jane A. Fagerland, Ph.D.
Abbott Laboratories
D-45M/AP31
200 Abbott Park Rd.
Abbott Park IL 60064-6202

You may also fax a resume to me at (847) 938-5027 or e-mail it to me at
jane.a.fagerland-at-abbott.com.

If you'd like further information, I can be reached by telephone at (847)
935-0104, or by e-mail. I prefer e-mail, but will be more than happy to talk
to discuss our projects and laboratory by telephone.



From daemon Wed Feb 09 23:52:01 2000



From: Walck. Scott D. :      walck-at-ppg.com
Date: Wed, 9 Feb 2000 20:31:58 -0500
Subject: RE: PIPS and milling damage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


There is an easy way to determine the milling damage between the two
machines and the amount of ion milling damage (subjectively); compare them
with samples prepared using the small angle cleavage technique. Look at
John McCaffrey's paper on using the small angle cleavage technique in
Ultramicrotomy 38, (149) 1991. He shows the difference between high angle
milling, low angle milling and the small angle cleavage technique. Of
course, the small angle cleavage technique showed no ion milling damage. It
is perfect for these types of materials. If you don't need a site specific
technique, it is the way to go for semiconductors.

You should also look at his paper in the MRS TEM Sample Prep series III (vol
254) that also shows a comparison for a SiGe/Si layer structure. We have a
detailed pictorial outline with tips and tricks on how to do it in the MRS
TEM Sample Prep series IV (vol 480).

I know that you invested a lot in your ion mill, but you can do these
samples very cheaply. SouthBay Technology sells a Microcleave kit that gets
you started with the technique.



Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)


"The opinions expressed are those of Scott D. Walck and not of PPG
Industries, Inc. nor of any PPG-associated companies."
--




} -----Original Message-----
} From: Massimo Catalano [mailto:massimo.catalano-at-ime.le.cnr.it]
} Sent: Wednesday, February 09, 2000 3:43 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: PIPS and milling damage
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.} html
}
}
} --------------------------------------------------------------
} ---------.
}
}
} Dear Listservers,
}
} we are massively working on analytical and structural TEM
} characterization
} of semiconductors, mainly InGaAs/GaAs, quantum well, wires and dots.
}
} Specimen preparation is of course a crucial point of our work.
}
} We have just switched to PIPS (we used to make our samples
} using the Gatan
} Duo Mill), and we are getting controversial results about the damage
} introduced by the milling procedure.
}
} We are perfectly aware of all the differences between the two
} instruments,
} especially the absence of cooling stage and the higher beam current.
}
} Has anyone performed a systematic and careful analysis to try
} to evaluate
} the milling damage on similar materials systems. We are
} willing to start a
} systematic work to assess this issue, but would like to know
} if anyone has
} already done anything on this topic. Also, any collaboration
} will be more
} than welcome.
}
} Thanks.
}
} Massimo
}
}
}
}
} Dr. Massimo Catalano
} CNR-IME
} Campus Universitario
} Via Arnesano
} 73100 Lecce - ITALY
} tel: + 39 0832 322362
} fax: + 39 0832 325299
} email: massimo.catalano-at-ime.le.cnr.it
} http://www.ime.le.cnr.it
} http://www.ime.le.cnr.it/sime/sime.htm
}
}
}



From daemon Wed Feb 09 23:52:03 2000



From: Barbara Foster :      mme-at-map.com
Date: Wed, 09 Feb 2000 20:34:34 -0500
Subject: Re: LM: Course reminder - DATES...

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi again,

It has been brought to my attention that no dates were attached to this
course reminder:

March 10-12, 2000
Hyatt Regency
New Orleans, LA.

Best regards,
Barbara Foster
Consortium President
Microscopy/Microscopy Education ...Educating microscopists for greater
productivity.

125 Paridon Street Suite 102 Springfield, MA 01118
PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com
Visit our web site {http://www.MME-Microscopy.com/education}



At 07:57 PM 2/7/00 -0500, Barbara Foster wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Wed Feb 09 23:52:05 2000



From: Barbara Foster :      mme-at-map.com
Date: Wed, 09 Feb 2000 20:39:10 -0500
Subject: Re: tacky wax

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Linda,

Suggest you contact Chuck Garber at Structure Probe: www.2spi.com
I'm sure that he has some sort of derivative of his tacky dots which would
be helpful


At 08:48 PM 8/27/70 +0000, Linda Chicoine wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Wed Feb 09 23:52:23 2000



From: Christina bennett :      chbennet-at-nmsu.edu
Date: Wed, 9 Feb 2000 21:58:18 -0700
Subject: FYI: Congress to allow email charges

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I know this is off topic however a change like this could make this type of
forum impossible.





} Date: Tue, 8 Feb 2000 08:13:53 -0700
} X-Sender: liperez-at-cnmailsvr.nmsu.edu
} Mime-Version: 1.0
} To: all-at-biology.nmsu.edu
} From: liperez-at-NMSU.Edu (LSPSaldana)
} Subject: Congress to allow email charges
} Sender: owner-all-at-biology.nmsu.edu
} Precedence: bulk
} X-Keywords:
} X-UID: 43
} Status: O
}
}
} } } Congress to allow email charges
} } }
} } } Please pass this on to all you know since many of us use e-mail for
} } } business and to keep up with friends and family, I thought you'd like
} } } to know the following.
} } }
} } } Please jump on it right away and forward this to others.
} } }
} } } CNN has reported that within the next two weeks Congress is going to
} } } vote on allowing telephone companies to CHARGE A TOLL FEE for
} } } internet access.
} } } Translation: Every time we send long distance e-mail we will receive a
} } } long distance charge. This will get costly. Please visit the following web
} } } site and file a complaint. Complain to your Congressperson. We can't
} } } allow this to pass. The following address will allow you to send an
} } } e-mail on this subject DIRECTLY to your Congressperson.
} } }
} } } http://www.house.gov/writerep {http://www.house.gov/writerep}
} } }
} } } Pass this on to your friends. It is urgent! I hope all of you will pass
} } } this on to all your friends and family. We should ALL have
} } } an interest in this one.
} } }
} } } WAIT, THERE'S MORE. IN ADDITION, The last few months have revealed an
} } } alarming trend in the Government of the United States attempting to
} } } quietly push through legislation that will affect your use of the
} } Internet.
} } } Under
} } } proposed legislation the U.S. Postal Service will be attempting to bill
} } } email users out of "alternate postage fees". Bill 602P will permit the
} } } Federal Govt. to charge a 5 cent surcharge on every email delivered, by
} } } billing Internet Service Providers at source. The consumer would then be
} } } billed in turn by the ISP. Washington D.C. lawyer Richard Stepp is
} } } working without pay to prevent this legislation from becoming law. The
} } U.S.
} } } Postal Service is claiming that lost revenue due to the proliferation of
} } } email
} } } is costing nearly $230,000,000 in revenue per year. You may have noticed
} } } their recent ad campaign "There is nothing like a letter".
} } } Since the average citizen received about 10 pieces of email per day in
} } } 1998,
} } } the cost to the typical individual would be an additional 50 cents per
} } } day, or over $180 dollars Per year, above and beyond there regular
} } Internet
} } } costs.
} } } Note that this would be money paid directly to the U.S. Postal Service
} } } for a service they do not even provide. The whole point of the Internet is
} } } democracy and non-interference. If the federal government is permitted
} } } to tamper with our liberties by adding a surcharge to email, who knows
} } Where
} } } it will end. You are already paying an exorbitant price for snail mail
} } } because of bureaucratic inefficiency. It currently takes up to 6 days for
} } a
} } } letter to be delivered from New York to Buffalo. If The U.S. Postal
} } Service
} } } is
} } } allowed to tinker with email; it will mark the end of the "free"
} } } Internet in the United States.
} } } One congressman, Tony Schnell has even suggested a "twenty to forty
} } } dollar per month surcharge on all internet service" above and beyond the
} } } government's proposed email Charges. Note that most of the major
} } } newspapers have ignored the story, the only exception being the
} } } Washingtonian
} } } which called the idea of email surcharge "a useful concept who's time has
} } } come"
} } } (March 6, 1999) Editorial.
} } }
} } } Don't sit by and watch your freedoms erode away! Send this e-mail to
} } } EVERYONE on your list, and tell all your friends and relatives to write
} } } to their congressman and say "No!" to Bill 602P.
} } }
} } }
} } } It will only take a few moments of your time, and could very well be
} } } instrumental in killing a bill we don't want.
} } }
} } } PASS THIS ON TO EVERYONE YOU KNOW WHO USES EMAIL REMEMBER THESE ARE TWO
} } } SEPARATE ISSUES THAT EFFECT ALL OF US ONLINE. LET YOU VOICE BE HEARD
} } } NOW, NOT AFTER
} } }
} } }
} } }
} } }
} } }
} } }
} }
}




From daemon Thu Feb 10 19:03:06 2000



From: S.A.Gusev :      gusev-at-ipm.sci-nnov.ru
Date: Thu, 10 Feb 2000 10:51:57 +0300
Subject: Solid State Electron Emitters

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Donald,
the Institute of Applied Physics
(http://www.sandy.ru/science/science/appl.new/frames.html)
constructs, produces and uses solid state high energy electron gun for
the High-power electronics and plasma physics researches.
May be they will help you.

Best regards,
Dr. S.A.Gusev


********************************************
* Institute for Physics of Microstrutures *
* Russian Academy of Science *
* ( IPM RAS ) *
* *
* Niznii Novgorod, GSP-105 *
* 603600 *
* RUSSIA *
* *
********************************************

tel: (+7)-8312-675313
fax: (+7)-8312-675553
e-mail: gusev-at-ipm.sci-nnov.ru




From daemon Thu Feb 10 19:03:12 2000



From: Erdem Yasar :      yasar-at-turkuaz.kku.edu.tr
Date: Thu, 10 Feb 2000 11:29:38 +0200 (EET)
Subject: about my water cooling system

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,
We have got a JEM3010 Transmission electron microscopy.I have got a
one problem about water cooling system.I dont
know,Which kind of water to used?I think so, We should use pure water for
water cooling?Besides My city water is not clean.What do you think about
this problem?
Thanks for your interest

**********************************
**********************************
** Research.Asst.ERDEM YASAR **
** University of Kirikkale **
** Department of Physics **
** TEM LABORATORY **
** 71450 KIRIKKALE/TURKEY **
** erdem.yasar-at-physics.org **
** yasar-at-science.ankara.edu.tr **
** yasar-at-turkuaz.kku.edu.tr **
**********************************
**********************************








From daemon Thu Feb 10 19:03:14 2000



From: Matt L Walker :      bmsmlw-at-bms.leeds.ac.uk
Date: Thu, 10 Feb 2000 10:08:40 -0000
Subject: TEM: Re Sarcomere cross-sections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I am looking for TEM images that show cross-sectional views of skeletal
muscle
sarcomeres during the *contracted state*. In particular, I am
interested in
seeing the spacing of the thin filaments when they **overlap each
other** (i.e.,
when those from one side of the sarcomere overlap those from the other
side of
the sarcomere in the contracted state). In addition, I am interested in
seeing
the distribution of the thin filaments as they pass through the M line.
It
would be greatly appreciated if anyone could tell me if they know of any
research papers or review articles that contain such TEM images.

Thank you!

Izak Paul
Biological Sciences
Mount Royal College

The following papers are classics and would make good starting point.
Huxley, H.E. & Hanson, J. (1954). Nature, 173, 973-976.
Huxley, A.F. & Niedergerke, R.M. (1954). Nature, 173, 971.
Huxley, H.E. (1957). Biophys. & Biochem. Cytol. 3, 631-648.

Thin filaments 'pass through' the Z-line. Try John Squire and Pradeep
Luther
(3D reconstruction of fish Z-disc) and Mike Reedy (insect) or Bellinda
Bullard.

Matt Walker
School of Biomedical Sciences
Leeds University, UK





From daemon Thu Feb 10 19:03:25 2000



From: Malcolm Haswell :      malcolm.haswell-at-sunderland.ac.uk
Date: Thu, 10 Feb 2000 11:26:59 +0000
Subject: Film scanners - that old chestnut

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I'm sorry to bring this topic up yet again, but I have just come across
the specification for a flat-bed scanner which looks as if it would be
suitable for scanning e.m. cut-film. It is the Epson Perfection 1200
Photo which includes a 5x4inch film adapter.

The UK web site address is:
http://www.epson.co.uk/sohoprod/imaging/scanner/perf1200/perf1200photo/spec.htm

and the specification is:
A4 Colour flatbed with 5"x4" film image scanner Single pass scanning
Optical resolution: 1200 x 2400 (30600 pixels/line)
Maximum output resolution of 9600x9600 dpi
36 bits per pixel in colour (24 bit output)
12 bits per pixel in black (8 bit output)
Optical Density 3.2D
USB (Type B)

Does anyone have any experience of this machine because it is about a
fifth of the price of the Umax flatbed film scanners? My only
reservation is that it is a SOHO product rather than professional.


Thanks in advance

Malcolm

Malcolm Haswell
Electron Microscopy
School of Sciences
Fleming Building
University of Sunderland
SUNDERLAND SR1 3SD
Tyne and Wear
UK

Tel (0191) 515 2872
e-mail: malcolm.haswell-at-sunderland.ac.uk



From daemon Thu Feb 10 19:04:04 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Thu, 10 Feb 2000 05:34:15 -0600
Subject: Re: FYI: Congress to allow email charges

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


This is a recurring post about nothing more than a hoax.

Search for internet hoaxes and so forth and you will find
this one and many other interesting yet equally invalid assertions.

gary g.


At 10:58 PM 2/9/00 , you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Thu Feb 10 19:03:44 2000



From: Jonathan Barnard :      Jonathan.Barnard-at-Angstrom.UU.SE
Date: Thu, 10 Feb 2000 13:38:12 +0100
Subject: coherence and spot size

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The statement that the smallest spot size gives the best coherence can be a
misleading. The full statement should be the spot size with the smallest
angular distribution should be used. Anyone who has tried to do off-axis
holography in nanoprobe mode will testify to this.
The spatial coherence envelope, roughly the distance over which the beam is
considered 'coherent', is the Fourier transform of the angular distribution
of intensity at the source (Van Cittert Vernike theorem).

Born & Wolf (section 10.4.2)
"Hence if the linear dimensions of the source and the distance between P1
and P2 (points in the imaging plane) are small compared to the distance of
these points from the source, the degree of coherence, |mu_12| is equal to
the absolute value of the normalized Fourier transform of the intensity
function of the source"

The reason holography and high resolution work is done with te most
parallel beam possible becomes clear, the angular distribution of a
converged (or diverged beam) is wide enough to reduce the spatial coherence
envelope. In off-axis holography this is even more stringent since two
interfering points (reference wave & object wave) can be hundreds of
nanometers, microns even, apart. Elliptical illumination is used to
preserve coherence in the one important direction (minimum angular width)
and converged in the other to improve the intensity.


********************************************************
Dr Jonathan Barnard

Analytical Materials Physics
The Angstrom Laboratory, Uppsala University
P O Box 534, SE-751 21 Uppsala, Sweden
Phone: +46-(0)18-4716838 Fax: +46-(0)18-500131
http://www.angstrom.uu.se/analytical/home.html

********************************************************



From daemon Thu Feb 10 19:03:46 2000



From: EvexAnalyt-at-aol.com
Date: Thu, 10 Feb 2000 07:49:14 EST
Subject: Re: FYI: Congress to allow email charges

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Christine,

I read the following just the other day, from the US Postal Service

http://www.usps.gov/news/press/99/99045new.htm


FOR IMMEDIATE RELEASE
May 21, 1999
Release No. 45

E-MAIL RUMOR COMPLETELY UNTRUE

WASHINGTON – A completely false rumor concerning the U.S. Postal Service is
being circulated on Internet e-mail. As a matter of fact, the Postal Service
has learned that a similar hoax occurred recently in Canada concerning Canada
Post.

The e-mail message claims that a "Congressman Schnell" has introduced "Bill
602P" to allow the federal government to impose a 5-cent surcharge on each
e-mail message delivered over the Internet. The money would be collected by
Internet Service Providers and then turned over to the Postal Service.

No such proposed legislation exists. In fact, no "Congressman Schnell" exists.

The U.S. Postal Service has no authority to surcharge e-mail messages sent
over the Internet, nor would it support such legislation.

-30-


Evex Analytical
X-ray Analyzers and Digital Imaging Systems
857 StateRoad
Princeton, NJ 08540
609-252-9192 T
609-252-9091 F
www.evex.com
info-at-evex.com




In a message dated 2/10/00 12:26:56 AM Eastern Standard Time,
chbennet-at-nmsu.edu writes:

{ { Subj: FYI: Congress to allow email charges
Date: 2/10/00 12:26:56 AM Eastern Standard Time
From: chbennet-at-nmsu.edu (Christina bennett)
To: microscopy-at-sparc5.microscopy.com

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America
To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
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I know this is off topic however a change like this could make this type of
forum impossible.





} Date: Tue, 8 Feb 2000 08:13:53 -0700
} X-Sender: liperez-at-cnmailsvr.nmsu.edu
} Mime-Version: 1.0
} To: all-at-biology.nmsu.edu
} From: liperez-at-NMSU.Edu (LSPSaldana)
} Subject: Congress to allow email charges
} Sender: owner-all-at-biology.nmsu.edu
} Precedence: bulk
} X-Keywords:
} X-UID: 43
} Status: O
}
}
} } } Congress to allow email charges
} } }
} } } Please pass this on to all you know since many of us use e-mail for
} } } business and to keep up with friends and family, I thought you'd like
} } } to know the following.
} } }
} } } Please jump on it right away and forward this to others.
} } }
} } } CNN has reported that within the next two weeks Congress is going to
} } } vote on allowing telephone companies to CHARGE A TOLL FEE for
} } } internet access.
} } } Translation: Every time we send long distance e-mail we will receive a
} } } long distance charge. This will get costly. Please visit the following
web
} } } site and file a complaint. Complain to your Congressperson. We can't
} } } allow this to pass. The following address will allow you to send an
} } } e-mail on this subject DIRECTLY to your Congressperson.
} } }
} } } http://www.house.gov/writerep {http://www.house.gov/writerep}
} } }
} } } Pass this on to your friends. It is urgent! I hope all of you will pass
} } } this on to all your friends and family. We should ALL have
} } } an interest in this one.
} } }
} } } WAIT, THERE'S MORE. IN ADDITION, The last few months have revealed an
} } } alarming trend in the Government of the United States attempting to
} } } quietly push through legislation that will affect your use of the
} } Internet.
} } } Under
} } } proposed legislation the U.S. Postal Service will be attempting to bill
} } } email users out of "alternate postage fees". Bill 602P will permit the
} } } Federal Govt. to charge a 5 cent surcharge on every email delivered, by
} } } billing Internet Service Providers at source. The consumer would then be
} } } billed in turn by the ISP. Washington D.C. lawyer Richard Stepp is
} } } working without pay to prevent this legislation from becoming law. The
} } U.S.
} } } Postal Service is claiming that lost revenue due to the proliferation of
} } } email
} } } is costing nearly $230,000,000 in revenue per year. You may have noticed
} } } their recent ad campaign "There is nothing like a letter".
} } } Since the average citizen received about 10 pieces of email per day in
} } } 1998,
} } } the cost to the typical individual would be an additional 50 cents per
} } } day, or over $180 dollars Per year, above and beyond there regular
} } Internet
} } } costs.
} } } Note that this would be money paid directly to the U.S. Postal Service
} } } for a service they do not even provide. The whole point of the Internet
is
} } } democracy and non-interference. If the federal government is permitted
} } } to tamper with our liberties by adding a surcharge to email, who knows
} } Where
} } } it will end. You are already paying an exorbitant price for snail mail
} } } because of bureaucratic inefficiency. It currently takes up to 6 days for
} } a
} } } letter to be delivered from New York to Buffalo. If The U.S. Postal
} } Service
} } } is
} } } allowed to tinker with email; it will mark the end of the "free"
} } } Internet in the United States.
} } } One congressman, Tony Schnell has even suggested a "twenty to forty
} } } dollar per month surcharge on all internet service" above and beyond the
} } } government's proposed email Charges. Note that most of the major
} } } newspapers have ignored the story, the only exception being the
} } } Washingtonian
} } } which called the idea of email surcharge "a useful concept who's time has
} } } come"
} } } (March 6, 1999) Editorial.
} } }
} } } Don't sit by and watch your freedoms erode away! Send this e-mail to
} } } EVERYONE on your list, and tell all your friends and relatives to write
} } } to their congressman and say "No!" to Bill 602P.
} } }
} } }
} } } It will only take a few moments of your time, and could very well be
} } } instrumental in killing a bill we don't want.
} } }
} } } PASS THIS ON TO EVERYONE YOU KNOW WHO USES EMAIL REMEMBER THESE ARE TWO
} } } SEPARATE ISSUES THAT EFFECT ALL OF US ONLINE. LET YOU VOICE BE HEARD
} } } NOW, NOT AFTER
} } }
} } }
} } }
} } }
} } }
} } }
} }
}





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Date: Wed, 9 Feb 2000 21:58:18 -0700
To: microscopy-at-sparc5.microscopy.com
From: Christina bennett {chbennet-at-nmsu.edu}
Subject: FYI: Congress to allow email charges
Errors-to: Microscopy-request-at-sparc5.Microscopy.Com

} }



From daemon Thu Feb 10 19:03:37 2000



From: White, Woody N :      Woody.N.White-at-mcdermott.com
Date: Thu, 10 Feb 2000 07:14:00 -0600
Subject: Re:FYI: Congress to allow email charges -Hoax?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Is this yet another hoax eating time & bandwidth by being posted without
confirmation (like the last 3-4 times I saw this message in the pas year or
two), or is it real?

I could find no reference to it at the FCC site.... Those people who are
REALLY
in charge of telecommunication regulations....

Woody

New format, more pix:
http://www.geocities.com/capecanaveral/3722



From daemon Thu Feb 10 19:03:38 2000



From: White, Woody N :      Woody.N.White-at-mcdermott.com
Date: Thu, 10 Feb 2000 07:29:00 -0600
Subject: Noise vs. Be

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Harry,

Weelll... For most systems, if the low end noise is not inhibited (most
are),
the noise generated, apparent x-ray intensity, tends to increase with lower
ev.
This would not produce a gaussian-like peak, but a monotonic rise in
intensity
with lower ev. OTOH, pulse processor circuitry *could* be designed/setup to
produce such an effect. Seeing Be x-rays from 100% element is difficult to
impossible with most EDS systems. If compounded, the odds get worse.

Woody



From daemon Thu Feb 10 19:03:53 2000



From: Alwyn Eades :      jae5-at-lehigh.edu
Date: Thu, 10 Feb 2000 08:29:03 -0500
Subject: Re: Solid State Electron Emitters

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In the early 1980's Philips believed that their research labs had
successfully developed a new electron source suitable for electron
microscopes. Indeed there was a time when they were planning to sell
microscopes with the new sources. I never heard what went wrong. There
were rumors that the lifetime was not good enough.

Anyone who wants details can find them in their library:
"An Efficient Silicon Cold Cathode for High Current Densities"
Van Gorkom and Hoeberechts
Philips Journal of Research 39 (1984) 51-60


Alwyn Eades
Department of Materials Science and Engineering
Lehigh University
5 East Packer Avenue
Bethlehem
Pennsylvannia 18015-3195
Phone 610 758 4231
Fax 610 758 4244
jae5-at-lehigh.edu



From daemon Thu Feb 10 19:03:49 2000



From: David_Bell-at-Millipore.com
Date: Thu, 10 Feb 2000 08:29:28 -0500
Subject: Re: FYI: Congress to allow email charges

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Hi all,

This, of course, is a classic internet hoax. I refer you to the following
website:


http://ciac.llnl.gov/ciac/CIACHoaxes.html#internetcharge

This hoax is designed, like many of the others, to eat up bandwidth and get
people involved in a general uproar.

Hopefully, no one panicked here! It may be worth the time to bookmark this hoax
site, or a similar one, so that when
we get messages such as this in our email, we can check their validity, before
contributing to its spread.
No insult intended towards anyone who falls victim to these emails, as I'm sure
most of us have at one time
or another.

Sincerely,

David Bell
Scientist
Electron Microscopy Lab
Millipore Corporation
80 Ashby Road
Bedford, MA 01730
(781) 533-2108




From daemon Thu Feb 10 19:04:09 2000



From: Scott Wight :      scott.wight-at-nist.gov
Date: Thu, 10 Feb 2000 08:54:18 -0500
Subject: Re: FYI: Congress to allow email charges

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


This is one of those internet hoaxes. It is not true, do not send it to
your friends, do not write your congressperson. Mining Co has a great web
page that debunks these hoaxes and myths
{http://urbanlegends.miningco.com/culture/urbanlegends/library/blhoax.htm?pid=27
33&cob=home} and is a good place to check before sending on any email that
urges you to send it to everyone you know. This message is a combination
of two old and popular hoaxes, see
{http://urbanlegends.miningco.com/culture/urbanlegends/library/blemtax2.htm}
and
{http://urbanlegends.about.com/culture/urbanlegends/library/weekly/aa012099.htm}
Hopefully this will die here.
Scott


} } } } Congress to allow email charges
} } } }
} } } } Please pass this on to all you know since many of us use e-mail for
} } } } business and to keep up with friends and family, I thought you'd like
} } } } to know the following.
} } } }
} } } } Please jump on it right away and forward this to others.
} } } }
} } } } CNN has reported that within the next two weeks Congress is going to
} } } } vote on allowing telephone companies to CHARGE A TOLL FEE for
} } } } internet access.
} } } } Translation: Every time we send long distance e-mail we will receive a
} } } } long distance charge. This will get costly. Please visit the following web
} } } } site and file a complaint. Complain to your Congressperson. We can't
} } } } allow this to pass. The following address will allow you to send an
} } } } e-mail on this subject DIRECTLY to your Congressperson.
} } } }
} } } } http://www.house.gov/writerep {http://www.house.gov/writerep}

..sniped...
} } } }
} } } } PASS THIS ON TO EVERYONE YOU KNOW WHO USES EMAIL REMEMBER THESE ARE TWO
} } } } SEPARATE ISSUES THAT EFFECT ALL OF US ONLINE. LET YOU VOICE BE HEARD
} } } } NOW, NOT AFTER


-------------------note: new mailing address----------------------
Scott Wight e-mail: scott.wight-at-nist.gov
NIST 222/A113 W voice: 301-975-3949
100 Bureau Dr STOP 8371 | fax: 301-417-1321
Gaithersburg, MD 20899-8371 \|/ disclaimer: Any opinion expressed
is my own and does not represent those of my employer.




From daemon Thu Feb 10 19:04:50 2000



From: William Janssen :      bjanssen-at-neuro.mssm.edu
Date: Thu, 10 Feb 2000 10:31:03 -0400
Subject: position available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


A technical position is available in the Neurobiology of Aging program at the
Mount Sinai School of Medicine in New York. We are seeking an experienced
Electron Microscopist with a BS/BA or MS in Biology/Life Sciences. Applicants
should have excellent communication and organizational skills, an understanding
of basic laboratory procedures, and the ability to manage a large and varied
workload. The successful candidate will participate in ultrastructural studies
focusing on the effects of: estrogen and aging on hippocampal circuitry
which is implicated in learning and memory, quantitative excitatory amino
acid receptor (NMDA and AMPA) distribution within the central nervous
system of transgenic models, as well as manipulated primate and rodent
models. Qualifications include at least 2 years of experience in routine
transmission electron microscopy procedures,
ultramicrotomy, immunogold labelling, specimen preparation, digital
photography, and routine maintenance of equipment. Experience with
immunofluorescence and confocal microscopy is an asset.

We offer a salary commensurate with experience and excellent benefits. For
consideration, please mail/email your resume to:

Bill Janssen
Neurobiology of Aging
Box 867, EB9-02
Mount Sinai School of Medicine,
One Gustave L. Levy Place,
New York, NY 10029-6574

We are an equal opportunity employer fostering diversity in the workplace.
Bill Janssen
Neurobiology of Aging Laboratories
Mount Sinai School of Medicine, Box 1639
One Gustave L. Levy Place
New York, NY 10029

Phone 212-824-8789
Fax 212-849-2510



From daemon Thu Feb 10 19:04:37 2000



From: A.Walker :      Alan.Walker-at-sheffield.ac.uk
Date: Thu, 10 Feb 2000 14:55:24 +0000
Subject: Re: about my water cooling system

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

I think the best solution would be to use a water recirculatory
system. Fill it with clean water and it should stay clean for a
long time. We use a 'Neslab'

Hope this helps

Alan Walker.

*********************************************
Alan Walker
Dept of Electronic and Electrical Engineering
University of Sheffield
Mappin Street
Sheffield S1 3JD
United Kingdom

Tel:+44-(0)114-2225365
Fax:+44-(0)114-2726391
alan.walker-at-shef.ac.uk
http://www.shef.ac.uk/uni/academic/D-H/eee/
*********************************************



From daemon Thu Feb 10 19:04:48 2000



From: Michael Bode :      mb-at-soft-imaging.com
Date: Thu, 10 Feb 2000 07:59:28 -0700
Subject: Re: Size determination of overlapping particles

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Rosemary,

this is of course a recurring theme in image analysis. There really is
no easy answer to this. Counting particles is easier as you can perhaps
separate the particles and arrive at the correct count, but if you don't
know what is occluded, you cannot measure it. However, if you can use
some information that is available to make some guesses or
extrapolations what the shape is, it can be done. A simple example: If
you look at a heap of coins, they may overlap. It is nevertheless
possible to measure the individual coins because I know that they are
all round. So I can take the "protruding" part of a coin and simply fit
a circle and that should give me the size pretty accurately.

Are ice crystals like snowflakes? Snowflakes, if I remember correctly,
have a six-fold symmetry. Can't you just measure one "branch" of a
snowflake (the one that you can see), and multiply that by 6? I am not
an expert on ice crystals (although I love to ski), so this may be
oversimplified. You may have to think about all the information you have
about ice crystals and can then perhaps make some guesses about the
occluded part.

Michael

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



} ----------
} From: Rosemary Walsh[SMTP:RW9-at-PSU.EDU]
} Sent: Tuesday, February 08, 2000 3:24:35 PM
} To: microscopy-at-sparc5.Microscopy.Com
} Subject: Size determination of overlapping particles
} Auto forwarded by a Rule
}
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Dear Listers,
I have a student interested in sizing ice crystals
which overlap. Any suggestions?
Rosemary




From daemon Thu Feb 10 19:04:13 2000



From: spatel-at-goodyear.com
Date: Thu, 10 Feb 2000 09:59:36 -0500
Subject: Liquid Nitrogen

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Fellow Subscribers;

We have a tedious task of liquid nitrogen transfer and fill-up on our
instruments such as SEMs, TEM and for cryo-ultramicrotomes. Is there any
compact unit on market which would condense nitrogen from air into liquid
form? I know that Liquid oxygen would be a problem. TIA;

Siddharth Patel



From daemon Thu Feb 10 19:04:51 2000



From: Eric Windsor :      Eric.Windsor-at-nist.gov
Date: Thu, 10 Feb 2000 10:10:27 -0500
Subject: Re: PIPS and milling damage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Massimo,

I just read the two excellent replies from Scott and Phillip and fully agree
with them. Small angle cleavage technique (SACT) and the tripod polisher are
two very useful TEM prep techniques. In my experience, SACT is easier to learn
than tripod polishing but tripodding is applicable to more materials and yields
larger thin areas than does SACT.

As Phillip mentions, low energy milling in the final step of the process is
extremely important for reducing artifacts. I would finish using the lowest
possible energy that still yields effective milling.

Another approach is to use reactive ion beam etching (RIBE) or chemically
assisted ion beam etching (CAIBE). In both these techniques a reactive gas
(usually Iodine) is used. In RIBE, iodine is actually ionized and is used as
the milling gas. In CAIBE, iodine gas is introduced into the milling chamber
directly adjacent to the sample during argon ion milling. Both these
techniques have been shown to eliminate the ion beam damage for binary type
III-V compound semiconductors containing InP. Chew and Cullis (Ultramicroscopy
23/1987/175-198) also report improvements of ion milled surfaces for ternary
type III-V semiconductors containing InGaAs (materials you mentioned) using
RIBE.

I believe that Gatan offers an optional CAIBE attachment for the PIPS. This
may be the easiest thing to try first. Remember that iodine is very corrosive
and can damage ion milling parts if not used properly. Check with Gatan for
their recommendations.

Hope this helps,

Eric W.

Eric S. Windsor
Physical Scientist
NIST
100 Bureau Dr. Stop 8371
Gaithersburg, MD 20899-8371
(301) 975-3930
Fax: (301) 417-1321
Eric.Windsor-at-NIST.Gov


On Feb 9 -at- 09:43 Massimo Catalano wrote:

Dear Listservers,
we are massively working on analytical and structural TEM characterization
of semiconductors, mainly InGaAs/GaAs, quantum well, wires and dots.
Specimen preparation is of course a crucial point of our work.
We have just switched to PIPS (we used to make our samples using the Gatan
Duo Mill), and we are getting controversial results about the damage
introduced by the milling procedure.
We are perfectly aware of all the differences between the two instruments,
especially the absence of cooling stage and the higher beam current.
Has anyone performed a systematic and careful analysis to try to evaluate
the milling damage on similar materials systems. We are willing to start a
systematic work to assess this issue, but would like to know if anyone has
already done anything on this topic. Also, any collaboration will be more
than welcome.
Thanks.
Massimo



Dr. Massimo Catalano
CNR-IME
Campus Universitario
Via Arnesano
73100 Lecce - ITALY
tel: + 39 0832 322362
fax: + 39 0832 325299
email: massimo.catalano-at-ime.le.cnr.it
http://www.ime.le.cnr.it
http://www.ime.le.cnr.it/sime/sime.htm



Eric S. Windsor
Physical Scientist
NIST
100 Bureau Dr. Stop 8371
Gaithersburg, MD 20899-8371
(301) 975-3930
Fax: (301) 417-1321
Eric.Windsor-at-NIST.Gov



From daemon Thu Feb 10 19:04:42 2000



From: Malcolm Haswell :      malcolm.haswell-at-sunderland.ac.uk
Date: Thu, 10 Feb 2000 15:12:21 +0000
Subject: Re: Film scanners - that old chestnut

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Maureen and anyone else who's interested

I have found the basic description on the US/Canada website so I assume
its available there too - see address below:
http://www.epson.com/cam_scan/scanners/perfection1200/index.html

The price inclusive of transparency adapter appears to be about 200 UK
pounds over here. There seems to be three models:
1200s (standard print scanner - SCSI interface)
1200u (standard print scanner - USB interface)
1200photo (standard print scanner + 5x4inch transparency adapter - USB
interface)
It's the last of these that is of interest.

Malcolm Haswell
------------------------------------------
Maureen Petersen wrote:
}
} Malcolm:
}
} Will you divulge what this gem costs? Do you know if it is available in the
} US?
}
} Thank you, Maureen Petersen
} Dept Plant Pathology
} University of Florida
} Gainensville, FL 32611
}
} I'm sorry to bring this topic up yet again, but I have just come across
} the specification for a flat-bed scanner which looks as if it would be
} suitable for scanning e.m. cut-film. It is the Epson Perfection 1200
} Photo which includes a 5x4inch film adapter.
}
} The UK web site address is:
} http://www.epson.co.uk/sohoprod/imaging/scanner/perf1200/perf1200photo/spec.
} htm
}
} and the specification is:
} A4 Colour flatbed with 5"x4" film image scanner Single pass scanning
} Optical resolution: 1200 x 2400 (30600 pixels/line)
} Maximum output resolution of 9600x9600 dpi
} 36 bits per pixel in colour (24 bit output)
} 12 bits per pixel in black (8 bit output)
} Optical Density 3.2D
} USB (Type B)
}
} Does anyone have any experience of this machine because it is about a
} fifth of the price of the Umax flatbed film scanners? My only
} reservation is that it is a SOHO product rather than professional.
}
} Thanks in advance
}
} Malcolm
}
} Malcolm Haswell
} Electron Microscopy
} School of Sciences
} Fleming Building
} University of Sunderland
} SUNDERLAND SR1 3SD
} Tyne and Wear
} UK
}
} Tel (0191) 515 2872
} e-mail: malcolm.haswell-at-sunderland.ac.uk



From daemon Thu Feb 10 19:06:23 2000



From: David Knecht :      knecht-at-uconn.edu
Date: Thu, 10 Feb 2000 10:23:04 -0500
Subject: Color vs. monochrome cameras

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am considering the purchase of a new digital camera. Some of the new
cameras like the Spot-RT and Magnafire have the option of running as three
shot color or one shot gray-scale acquisition. If someone wants to do dual
channel fluorescence like FITC/Rhod or annexin/PI, we can do that as either
acquiring each image with a separate filter cube and combining in software,
or designing a dual filter cube and acquiring in color (or I guess
acquiring separate color images iwth the single cubes and combining??).
What is people real world experience with the relative merits of these two
approaches. It seems that most of what I read is done by separate filter
cubes combined in software, but there also seems to be a push toward fast
cooled color cameras. The advantage I can see to the color approach would
be using a color chip camera you get multiple channels simultaneously.
However, do you have to expose significantly longer to acheive this
compared to multiple gray scale acquisitions? I can't see an obvious
advantage to the three shot color approach because I suspect the light
throughput is lower and the filters sets I suspect are expensive to add on
if you already have the single fluor filters. The only real advantage I
see to three shot acquisition is the ability to easily image
stained/histology transmitted light slides and not for fluorescence. Any
thoughts appreciated. Thanks- Dave


************************************************************

Dr. David Knecht
Department of Molecular and Cell Biology
University of Connecticut
75 N. Eagleville Rd. U-125
Storrs, CT 06269
Knecht-at-uconnvm.uconn.edu
860-486-2200 860-486-4331 (fax)
home page: http://www.sp.uconn.edu/~mcbstaff/knecht/knecht.html
************************************************************




From daemon Thu Feb 10 19:04:58 2000



From: Kalman Rubinson :      kr4-at-is2.nyu.edu
Date: Thu, 10 Feb 2000 10:38:48 -0500 (EST)
Subject: Re: FYI: Congress to allow email charges

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


This nonsense resurfaces every few months. There has
never been any truth to it. (Of course, there's no
assurance that Congress will not act so foolishly in the
future.)

Kal

On Wed, 9 Feb 2000, Christina bennett wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I know this is off topic however a change like this could make this type of
} forum impossible.
}
}
}
}
}
} } Date: Tue, 8 Feb 2000 08:13:53 -0700
} } X-Sender: liperez-at-cnmailsvr.nmsu.edu
} } Mime-Version: 1.0
} } To: all-at-biology.nmsu.edu
} } From: liperez-at-NMSU.Edu (LSPSaldana)
} } Subject: Congress to allow email charges
} } Sender: owner-all-at-biology.nmsu.edu
} } Precedence: bulk
} } X-Keywords:
} } X-UID: 43
} } Status: O
} }
} }
} } } } Congress to allow email charges
} } } }
} } } } Please pass this on to all you know since many of us use e-mail for
} } } } business and to keep up with friends and family, I thought you'd like
} } } } to know the following.
} } } }
} } } } Please jump on it right away and forward this to others.
} } } }
} } } } CNN has reported that within the next two weeks Congress is going to
} } } } vote on allowing telephone companies to CHARGE A TOLL FEE for
} } } } internet access.
} } } } Translation: Every time we send long distance e-mail we will receive a
} } } } long distance charge. This will get costly. Please visit the following web
} } } } site and file a complaint. Complain to your Congressperson. We can't
} } } } allow this to pass. The following address will allow you to send an
} } } } e-mail on this subject DIRECTLY to your Congressperson.
} } } }
} } } } http://www.house.gov/writerep {http://www.house.gov/writerep}
} } } }
} } } } Pass this on to your friends. It is urgent! I hope all of you will pass
} } } } this on to all your friends and family. We should ALL have
} } } } an interest in this one.
} } } }
} } } } WAIT, THERE'S MORE. IN ADDITION, The last few months have revealed an
} } } } alarming trend in the Government of the United States attempting to
} } } } quietly push through legislation that will affect your use of the
} } } Internet.
} } } } Under
} } } } proposed legislation the U.S. Postal Service will be attempting to bill
} } } } email users out of "alternate postage fees". Bill 602P will permit the
} } } } Federal Govt. to charge a 5 cent surcharge on every email delivered, by
} } } } billing Internet Service Providers at source. The consumer would then be
} } } } billed in turn by the ISP. Washington D.C. lawyer Richard Stepp is
} } } } working without pay to prevent this legislation from becoming law. The
} } } U.S.
} } } } Postal Service is claiming that lost revenue due to the proliferation of
} } } } email
} } } } is costing nearly $230,000,000 in revenue per year. You may have noticed
} } } } their recent ad campaign "There is nothing like a letter".
} } } } Since the average citizen received about 10 pieces of email per day in
} } } } 1998,
} } } } the cost to the typical individual would be an additional 50 cents per
} } } } day, or over $180 dollars Per year, above and beyond there regular
} } } Internet
} } } } costs.
} } } } Note that this would be money paid directly to the U.S. Postal Service
} } } } for a service they do not even provide. The whole point of the Internet is
} } } } democracy and non-interference. If the federal government is permitted
} } } } to tamper with our liberties by adding a surcharge to email, who knows
} } } Where
} } } } it will end. You are already paying an exorbitant price for snail mail
} } } } because of bureaucratic inefficiency. It currently takes up to 6 days for
} } } a
} } } } letter to be delivered from New York to Buffalo. If The U.S. Postal
} } } Service
} } } } is
} } } } allowed to tinker with email; it will mark the end of the "free"
} } } } Internet in the United States.
} } } } One congressman, Tony Schnell has even suggested a "twenty to forty
} } } } dollar per month surcharge on all internet service" above and beyond the
} } } } government's proposed email Charges. Note that most of the major
} } } } newspapers have ignored the story, the only exception being the
} } } } Washingtonian
} } } } which called the idea of email surcharge "a useful concept who's time has
} } } } come"
} } } } (March 6, 1999) Editorial.
} } } }
} } } } Don't sit by and watch your freedoms erode away! Send this e-mail to
} } } } EVERYONE on your list, and tell all your friends and relatives to write
} } } } to their congressman and say "No!" to Bill 602P.
} } } }
} } } }
} } } } It will only take a few moments of your time, and could very well be
} } } } instrumental in killing a bill we don't want.
} } } }
} } } } PASS THIS ON TO EVERYONE YOU KNOW WHO USES EMAIL REMEMBER THESE ARE TWO
} } } } SEPARATE ISSUES THAT EFFECT ALL OF US ONLINE. LET YOU VOICE BE HEARD
} } } } NOW, NOT AFTER
} } } }
} } } }
} } } }
} } } }
} } } }
} } } }
} } }
} }
}
}
}





From daemon Thu Feb 10 19:05:08 2000



From: David_Bell-at-Millipore.com
Date: Thu, 10 Feb 2000 10:50:30 -0500
Subject: Information on RG Lee SEM's

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Colleagues,

Someone at a remote location within our company has decided to purchase an RG
Lee SEM. I am looking for information and opinions from people who have one of
these microscopes. I'm interested in any service issues, reliability issues and
usability concerns that anyone may have. The model they are interested in is
the PSM 75LS. Also, if anyone has the URL for their website, that would be
appreciated, as well.

Thanks in advance,

David Bell
Scientist
Electron Microscopy Lab
Millipore Corporation
80 Ashby Road
Bedford, MA 01730
(781) 533-2108




From daemon Thu Feb 10 19:05:17 2000



From: Hans Dijkstra :      hans.dijkstra-at-edax.nl
Date: Thu, 10 Feb 2000 17:00:49 +0100
Subject: RE: Be X-ray peaks -I don't think so

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Scott,

A poster does not mean anything. I have seen them with the Lithium-Ka energy
listed, although quantum physics does forbid this X-ray line. But I can
assure you the Be-K line does exist.

On modern EDX systems Be can be detected, provided you have either a
windowless detector or a detector with a modern polymer type window (SUTW,
SATW, Norvar, or whatever the EDX supplier calls it. My apologies if I break
any trade-marks here...).
On most demonstration setups your EDS specialist will be pleased to show you
a Be peak. But probably from a pure Be sample only. The absorption
coefficient (MAC) of Be in basically any matrix is so huge, and the X-ray
fluorescence yield so small, that in any compound with less than 50 atomic
percent Be you will not detect a visible Be peak. For this reason most EDX
installations "in the field" are not setup to detect Be-K radiation.

WDS has a better P/B ratio, so if you have the proper multilayer crystal you
can get results. But peak shifts and peak shape alterations will make
quantification extremely difficult, not to mention the fact that for most
MACs of Be-K in any matrix we only have a ball-park figure.

Other techniques, like PEELS, are much more suitable for Li and Be analysis.
Best regards,

Hans Dijkstra

Disclaimer: This is my opinion, and not necessarily the one from EDAX Inc.
-------------------------------------------------------------
EDAX Europe www.edax.com
Ringbaan Noord 103 Tel.: +31-13-5364000
P.O. Box 4144 Fax.: +31-13-5356279
5004 JC Tilburg E-mail: hans.dijkstra-at-edax.nl
the Netherlands
-------------------------------------------------------------


} -----Original Message-----
} From: Walck. Scott D. [mailto:walck-at-ppg.com]
} Sent: Tuesday, February 08, 2000 6:57 PM
} To: 'microprobe'; 'harry.ekstrom-at-honeywell.com'
} Cc: 'Microscopy'
} Subject: RE: Be X-ray peaks -I don't think so
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I'm wrong. I guess I should have looked up at the X-ray periodic table
} above my head and saw the 0.108 keV value for Be. The reason that I was
} wrong was that I did not consider the solid state aspects. I am still
right
} about not having a 2s1/2 to a 1s1/2 transition and that an X-ray carries
} away 1h-bar of angular momentum. What I didn't do was think about the
band
} structure of a solid. If you have the solid, the 2p bands of Be most be
} spilling into or be the conduction band for the electrons. That must be
the
} source for the electronic transition. I guess I should have engaged brain
} before typing.
} My most humble apologies.
} -Scott
}
} Scott D. Walck, Ph.D.
} PPG Industries, Inc.
} Glass Technology Center
} Guys Run Rd. (packages)
} P. O. Box 11472 (letters)
} Pittsburgh, PA 15238-0472
}
} Walck-at-PPG.com
}
} (412) 820-8651 (office)
} (412) 820-8161 (fax)
}
}
} "The opinions expressed are those of Scott D. Walck and not of PPG
} Industries, Inc. nor of any PPG-associated companies."
} --
}



From daemon Thu Feb 10 19:05:23 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Thu, 10 Feb 2000 08:08:10 -0800
Subject: urban myths and legends - Congress to allow e-mail charges

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Here are two URLs showing that this is just another hoax;


http://www.urbanmyths.com/email_internettax602p.html



http://www.ulrc.com.au/html/report.asp?CaseFile=ULRR0027&Page=1&View=Request

gary g.



From daemon Thu Feb 10 19:05:29 2000



From: sherwood-at-helix.mgh.harvard.edu (Peggy Sherwood)
Date: Thu, 10 Feb 2000 11:16:00 -0500
Subject: New England Society for Microscopy-March 8th Meeting Announcement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


To all:

The first meeting of the millenium for NESM (New England Society for
Microscopy) will be held on
March 8, 2000 at Polaroid Corporation in Wayland, MA. Registration will
begin at 5:00pm with tours
of the (new) facility following at 5:30pm. Attendees will also be asked to
fill outa questionnaire (optional) on "Professional Imaging" at this time.

A buffet supper will be served from 6-7pm followed by three scientific
presentations. The speakers
are : Gabriel Rojano, Staff Reliability Engineer-MIA-Com (Tyco Electronics
Company), Paul Bain from the Harvard Medical Library and Dr. Hjalmar
Brismer from the Karolinska Institute in Stockhold, Sweden.

Advance registration is encouraged (by March 3rd). The registration fee
for members is $5.00 and $20.00 for non-members (this includes a current
one-year membership in the society). Interested parties should contact:
Peggy Sherwood, Corresponding Secretary (MESnesm-at-aol.com).






From daemon Thu Feb 10 19:05:38 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Thu, 10 Feb 2000 08:29:18 -0800
Subject: Re: Congress to allow e-mail charges

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Different subject title in case other posting got filtered out.

} Date: Thu, 10 Feb 2000 08:08:10 -0800
} To: Microscopy-at-MSA.Microscopy.Com
} From: "Dr. Gary Gaugler" {gary-at-gaugler.com}
} Subject: urban myths and legends - Congress to allow e-mail charges
}
} Here are two URLs showing that this is just another hoax;
}
}
} http://www.urbanmyths.com/email_internettax602p.html
}
}
}
} http://www.ulrc.com.au/html/report.asp?CaseFile=ULRR0027&Page=1&View=Request
}
} gary g.



From daemon Thu Feb 10 19:05:43 2000



From: donald j marshall :      dmrelion-at-world.std.com
Date: Thu, 10 Feb 2000 11:48:39 -0500 (EST)
Subject: Congress to allow email charges

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The insidious nature of this hoax/scam/... is that not only does the
original hoax get circulated, using up bandwidth, but then the replies of
the people who identified the hoax, many with the full hoax message
attached, also get circulated. And then the triple play (baseball and spring
training is near) is when people like me comment on the aforesaid. Please,
please don't reply to this note (which should have no attachments!) and make
it a home run!

Don Marshall

Donald J. Marshall
Relion Industries
P.O. Box 12
Bedford, MA 01730
Ph: 781-275-4695
FAX: 781-271-0252
email dmrelion-at-world.std.com

Cathodoluminescence, mass spectroscopy, electron beam technology


"A weed is a flower out of place."

(Please note: Do not send email with attachments to this address. Instead, send it to donbarlen-at-aol.com. Thank you.)



From daemon Thu Feb 10 19:05:45 2000



From: Laurie Wallin :      lwallin-at-ucsd.edu
Date: Thu, 10 Feb 2000 09:00:52 -0800
Subject: RE: FYI: Congress to allow email charges

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


This is not true. It is an internet hoax. Here's the info from an urban
legends website:
---------------------------
Claim: Congress will soon be voting on whether or not your phone company
will be
allowed to charge you long-distance rates for accessing the Internet.

Status: False.

Example: [Collected on the Internet, 1999]

Please forward this to everyone you can...

There is a new bill in the US Congress that will affect ALL INTERNET
USERS. CNN stated that the Government would in two weeks time
decide whether to allow or not allow a Charge to YOUR phone bill equal
to a long distance call each time you access the Internet.

This affects us all! We cannot allow this to happen! Please visit the
following URL and fill out the necessary form to let your Congressman
know how you feel! The address is http://www.house.gov/writerep/.
Write
your representative!

Synopsis: Neither the FCC nor Congress is considering -- much less
voting on --
legislation that would impose (or allow phone companies to impose)
per-minute access fees
on Internet users. Recent decisions by the FCC in this area have dealt
only with the issue of
how phone companies reimburse each other for handling calls placed to
Internet service
providers, not with the prospect of allowing phone companies to charge
their customers
long-distance rates for Internet use.

Origins: As soon as we get hold of something we really like at a
reasonable price, we start
worrying that it will be banned, taxed, or made too expensive for us to
afford. The Internet is
no exception, as our old friend -- the capitalized, exclamation pointed,
"send this to everyone
you know" anonymous e-mail message -- is here to tell you.

Way back when in 1987, the Federal Communications Commission did
consider imposing a
surcharge for transmitting data over the public telephone network, but
they ultimately
rejected the idea (thanks in part to the more than 10,000 letters of
complaint they received).
Unable to believe our good fortune (the government wasn't going to make
us pay through
the nose for dialing up all those neat computer bulletin boards we'd
discovered), we couldn't
leave well enough alone, and in 1991 a flood of urgent messages warning
us that the FCC
was again considering a proposal they'd rejected three years earlier hit
e-mail systems all
over the country (and the nascently popular Internet). Like the
ubiquitous Craig Shergold
message, the "modem tax" warning would long outlive the validity of the
information it
conveyed.

Fast forward to 1998. On-line services, the Internet, the World Wide
Web, e-mail, and chat
rooms are more popular than ever, a daily part of many people's lives.
Somebody -- the
government, the phone companies, Bill Gates, the Grinch -- must be on
his way to spoil the
party. Sure enough, we're now being told the phone companies and the
government are in
cahoots to ruin our good time.

First of all, a little background. Most of us still have to dial up over
a modem and connect to
an Internet Service Provider (ISP) to access the Internet. If your ISP
is in your local dialing
area, you probably don't pay anything at all for the call, no matter how
long you stay
connected. This means you get to tie up a phone line with your modem for
hours and hours
on end, every day, at no charge beyond the price of basic phone service.
And the party at the
other end of the line -- your ISP -- isn't paying anything extra,
either. It's easy to see that
somebody has a chance to reap some windfall profits here. If the phone
companies were
allowed to charge you a per-minute fee for accessing the Internet (or
the government were
allowed to tax your use of the Internet), their coffers would soon
overflow with cash.

Scary thought, isn't it? All the phone companies need, we hear, is to
get the FCC to
reclassify and/or regulate ISPs, and then the phone companies can charge
gobs of extra
money for handling Internet traffic. And Congress is just about to vote
on that very issue,
we're told.

In fact, there is no such proposal before Congress, and there never has
been.

The only real issue before the FCC concerning Internet usage (and the
genesis of this latest
round of scaremongering) is the subject of "reciprocal compensation." In
short, reciprocal
compensation means that when you place a local call to someone who is
serviced by a
different phone company, your phone company has to compensate his phone
company for
completing the call. (On the other hand, when you place a long-distance
call, the long
distance carrier who handles the traffic has to pay access charges to
your phone company
for originating the call.) But if the "person" you're calling is an ISP,
should your phone
company have to compensate the ISP's phone company?

The subject of recpirocal compensation has been a hot issue of late
because new local phone
companies have been springing up all over the place. The bigger phone
companies, figuring
that they would have many more customers than their newer (and smaller)
competitors,
negotiated reciprocal compensation agreements with the new phone
companies. Every time
one of these little phone companies' customers placed a call to a
destination outside his local
service that ended up on the bigger phone company's network, the big
phone company
would get to collect money from the little phone company. Not a bad
scheme, the big phone
companies thought.

Ah, but some of the little guys had a neat trick up their sleeves. They
started offering their
services to Internet service providers -- ISPs with banks and banks of
modems that received
thousands and thousands of calls every day, but never made any outgoing
calls. All the
reciprocal compensation started flowing one direction, from the big
phone companies to the
little phone companies, which wasn't what the big guys had in mind at
all. "Foul," they cried.
"Internet traffic flows all over the world," they noted. "Internet
traffic is therefore interstate in
nature and should be classified as long-distance, so the little guys
should be paying us for
originating the calls," they insisted. "We're not paying," they sputtered.

Enter the FCC to resolve the dispute, which they did (for now) on 25
February 1999 by
ruling that phone companies are bound by whatever reciprocal
compensation agreements
they've negotiated with each other, whether they think they're fair or
not. That was the only
issue before the FCC. But most of us are already struggling with a glut
of information, and
we don't have the time to familiarize ourselves with details like
interconnection agreements
and reciprocal compensation, so when we hear reports with buzzwords like
"Internet,"
"FCC," "access fees," and "long distance," we assume the worst, even
though the real story
is something quite different. And even if we make the effort to
understand the whole story,
we find all too often that we're reading information that has been
misreported by others --
often the mainstream media -- who didn't make enough of an effort to
understand the whole
story themselves. If we can't even depend upon the people whose business
it is to supply us
with accurate information to get the facts straight, what more can we
do? (See, for example,
the misleading headline on the CNN article referenced in the "Additional
information"
section below.)

A few important points related to the recent FCC decision:

Didn't the FCC rule that Internet connections are long-distance
calls?

Sort of. The FCC declared that "Internet traffic is
jurisdictionally mixed and appears to
be largely interstate in nature," which is the technical
definition of "long distance." But
that doesn't mean -- as is often misreported -- that Internet
users will be paying long
distance rates for dial-up connections, since the Internet has
been, and still is, exempt
from interstate access charges. The FCC did nothing to abolish
that exemption.

Won't the phone companies just pass the cost of carrying Internet
traffic to
customers by raising their phone rates?

There is no guarantee that phone rates won't go up in the future,
of course. However,
since most states require phone companies to charge a flat rate
for unlimited local
usage, you won't have to pay per-minute charges for accessing the
Internet (as long as
your ISP has a dial-up number within your local service area).

What if the FCC changes their mind?

The possibility exists that the FCC might someday decide that
additional fees can be
imposed for Internet access. But as Bill Kennard, the chairman of
the FCC, has stated
on more than one occasion: "I want to say this as clearly as I can
. . . as long as I'm
chairman of the Federal Communications Commission this agency will
not regulate
the Internet. It's not going to happen. The FCC has no intention
of making computer
users pay long distance fees for dial-up access to the Internet,
as people now pay when
they make long-distance telephone calls. These rumors get on the
Internet that the big
bad FCC is going to impose all this regulation on the Internet.
Now I know this
painfully because every so often when one of these rumors flares
up I get, literally,
about 600 e-mail messages a day by people who are telling me to
keep my hands off
the Internet."
[Note: this is not a direct quote from Kennard; it is pieced
together from multiple
statements of his.]

Additional information:

No Consumer Per-Minute Charges to Access ISPs (FCC)

Users, Advertisers Await FCC Decision on Internet Charges
(CNN)

Update: In April 1999, a Canadian version of this message was
unleashed on the Internet.
Unlike its American counterpart, this version is not mere misinformation
based on a flawed
understanding of actual events or legislation -- it is an outright hoax:

Please read the following carefully if you intend to stay
online and continue using email:

The last few months have revealed an alarming trend in the
Government of Canada attempting to quietly push through
legislation that will affect your use of the Internet. Under
proposed legislation Canada Post will be attempting to bilk
email users out of "alternate postage fees".

Bill 602P will permit the Federal Govt to charge a 5 cent
surcharge on every email delivered, by billing Internet
Service Providers at source. The consumer would then be
billed in turn by the ISP. Toronto lawyer Richard Stepp QC is
working without pay to prevent this legislation from
becoming law.

The Canada Post Corporation is claiming that lost revenue
due to the proliferation of email is costing nearly
$23,000,000 in revenue per year. You may have noticed
Canada Post's recent ad campaign "There is nothing like a
letter". Since the average citizen received about 10 pieces
of email per day in 1998, the cost to the typical individual
would be an additional 50 cents per day, or over $180
dollars per year, above and beyond their regular Internet
costs. Note that this would be money paid directly to
Canada Post for a service they do not even provide. The
whole point of the Internet is democracy and
non-interference. If the Canadian Government is permitted
to tamper with our liberties by adding a surcharge to email,
who knows where it will end. You are already paying an
exhorbitant price for snail mail because of beaurocratic
inefficiency. It currently takes up to 6 days for a letter to be
delivered from Mississauga to Scarborough. If Canada Post
Corporation is allowed to tinker with email, it will mark the
end of the "free" Internet in Canada. One back-bencher,
Liberal Tony Schnell (NB) has even suggested a "twenty to
forty dollar per month surcharge on all Internet service"
above and beyond the government's proposed email
charges. Note that most of the major newspapers have
ignored the story, the only exception being the Toronto Star
that called the idea of email surcharge "a useful concept
who's time has come" (March 6th 1999 Editorial) Don't sit
by and watch your freedoms erode away! Send this email to
all Canadians on your list and tell your friends and relatives
to write to their MP and say "No!" to Bill 602P.

Kate Turner
Assistant to Richard Stepp QC
Berger, Stepp and Gorman
Barristers at Law
216 Bay Street
Toronto, ON
MlL 3C6

Yes, Americans are not alone in their belief that when the need for the
primary service
provided by a governmental agency diminishes or disappears, that agency
will come up with
draconian schemes to perpetuate its existence at taxpayer expense rather
than modernizing
or closing up shop. In this case, however, the message is simply too
riddled with errors to be
anything but a hoax:

There is no "Bill 602P" currently before the Canadian parliament.
The designations of
Canadian parliamentary bills take the form of the letter 'C' or
'S' followed by a
number (depending upon whether they originated in the House of
Commons or the
Senate). Besides having the wrong prefix, this purported bill is
assigned a number far
too high to be one currently being considered in parliament, as
you can see on the list
of Canadian government bills.

Despite the claim in the message, nothing about this alleged bill
is to be found. Also,
there was no editorial about this on the 6 March 1999 OpEd page of
Toronto
Star. (Maybe "major papers have ignored the story" because it's a
work of
fiction?)

There is no Richard Stepp QC; law firm by the name of Berger,
Stepp and Gorman; or
216 Bay Street in Toronto.

A list of Canadian Members of Parliament contains no MP by the
name of Tony
Schnell.

Of course, it didn't take long before the same thing started circulating
in an Americanized
version:

Dear Internet Subscriber:

Please read the following carefully if you intend to stay
online and continue using email: The last few months have
revealed an alarming trend in the Government of the United
States attempting to quietly push through legislation that
will affect your use of the Internet. Under proposed
legislation the U.S. Postal Service will be attempting to bilk
email users out of "alternate postage fees".

Bill 602P will permit the Federal Govt to charge a 5 cent
surcharge on every email delivered, by billing Internet
Service Providers at source. The consumer would then be
billed in turn by the ISP. Washington D.C. lawyer Richard
Stepp is working without pay to prevent this legislation
from becoming law.

The U.S. Postal Service is claiming that lost revenue due to
the proliferation of email is costing nearly $230,000,000 in
revenue per year. You may have noticed their recent ad
campaign "There is nothing like a letter". Since the average
citizen received about 10 pieces of email per day in 1998,
the cost to the typical individual would be an additional
50 cents per day, or over $180 dollars per year, above and
beyond their regular Internet costs. Note that this would be
money paid directly to the U.S. Postal Service for a service
they do not even provide. The whole point of the Internet is
democracy and non-interference. If the federal government
is permitted to tamper with our liberties by adding a
surcharge to email, who knows where it will end. You are
already paying an exorbitant price for snail mail because of
bureacratic efficiency. It currently takes up to 6 days for a
letter to be delivered from New York to Buffalo. If the U.S.
Postal Service is allowed to tinker with email, it will mark
the end of the "free" Internet in the United States. One
congressman, Tony Schnell (r) has even suggested a
"twenty to forty dollar per month surcharge on all Internet
service" above and beyond the government's proposed
email charges. Note that most of the major newspapers
have ignored the story, the only exception being the
Washingtonian which called the idea of email surcharge "a
useful concept who's time has come" (March 6th 1999
Editorial) Don't sit by and watch your freedoms erode away!

Send this email to all Americans on your list and tell your
friends and relatives to write to their congressman and say
"No!" to Bill 602P.

Kate Turner
Assistant to Richard Stepp
Berger, Stepp and Gorman
Attorneys at Law
216 Concorde Street,
Vienna, Va.



From daemon Thu Feb 10 19:05:48 2000



From: Laurie Wallin :      lwallin-at-ucsd.edu
Date: Thu, 10 Feb 2000 09:03:00 -0800
Subject: paraffin sectioning troubleshooting guide

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does anyone know where I can find a troubleshooting manual/guide for
paraffin sectioning? I am having trouble with the tissue "smearing" and
with it looking chopped-up. Is this a vibration issue? I welcome any
comments from those of you with experience in this area!

Thanks in advance.

Sincerely.



From daemon Thu Feb 10 19:05:51 2000



From: Laurie Wallin :      lwallin-at-ucsd.edu
Date: Thu, 10 Feb 2000 09:04:17 -0800
Subject: Fwd: paraffin sectioning troubleshooting guide

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Sorry, I forgot to sign my note last time...


} Date: Thu, 10 Feb 2000 09:03:00 -0800
} To: microscopy-at-sparc5.microscopy.com
} From: Laurie Wallin {lwallin-at-ucsd.edu}
} Subject: paraffin sectioning troubleshooting guide
} Cc:
} Bcc:
} X-Attachments:
}
} Does anyone know where I can find a troubleshooting manual/guide for
} paraffin sectioning? I am having trouble with the tissue "smearing" and
} with it looking chopped-up. Is this a vibration issue? I welcome any
} comments from those of you with experience in this area!
}
} Thanks in advance.
}
} Sincerely.

Laurie Wallin
UCSD Department of Anesthesiology
9500 Gilman Drive, 0629, La Jolla, CA 92093
(858) 822-3271



From daemon Thu Feb 10 19:06:00 2000



From: David Henriks :      Henriks-at-CompuServe.COM
Date: Thu, 10 Feb 2000 12:31:07 -0500
Subject: PIPS and milling damage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Dr. Catalano:

There has been some very significant work done by Dr. Arpad Barna et al in
Hungary. Some of the papers he has presented include:

Ion Energy Effect on Surface Amorphisation of Semiconductor Crystals/A.
Barna, et al

Amorphisation and Surface Morphology Development at Low Energy Ion
Milling/A. Barna, B. Pecz.

Analysis of the Development of Large Surface Topography During Ion
Etching/A. Barna; P. Barna; et al

Model Considerations of Ion Beam Thinning for Preparing TEM
Samples/A.Barna; et al

Possibility of Surface Polishing by Ion Beam Thinning/ A. Barna

Ion Beam Thinning on the Basis of Topographic Kinetics/A. Barna

Low Angle and Low Energy Ion Beam Etching for TEM Sample Preparation/A.
Barna

TEM Sample Preparation by Ion Milling / Amorphization/A. Barna, B. Pecz, M.
Menyhard

I also have the following paper that may be of interest:

Preparation of InGaAs/GaAs Multilayered Materials for TEM by One Side
Non-Rotation Ion Beam Thinning/J.Y. Yao; G.L. Dunlop

I do not have the complete references available in front of me, but we do
have copies of all of these papers in our technical library. I would be
pleased to mail copies to you if that would be of interest. We also have a
list of over 250 papers dealing with various aspects of sample preparation
(much of it TEM sample preparation) which may be of interest. If you would
like to review that list, I can send it over to you in MS Excel format and
you can select any other papers that would be of interest.

I hope this helps.

DISCLAIMER: South Bay Technology, Inc. markets the IV3 Ion Mill in both
high and low energy (down to 200ev) versions which is based on the work of
Dr. Barna. We also produce the XLA 2000 computer controlled Low Angle Ion
Mill so we have a vested interest in promoting their use.

Best regards-

David
Writing at 10:05:16 AM on 02/09/2000

***************************************************************************
************************

David Henriks
Vice President TEL: 800-728-2233
(toll free in the USA)
South Bay Technology, Inc. +1-949-492-2600
1120 Via Callejon FAX: +1-949-492-1499
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

***************************************************************************
************************

} } } } } Please visit us at http://www.southbaytech.com { { { { {

Manufacturers of precision sample preparation equipment and supplies for
metallography, crystallography and electron microscopy.

Message text written by Massimo Catalano
}
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Dear Listservers,

we are massively working on analytical and structural TEM characterization
of semiconductors, mainly InGaAs/GaAs, quantum well, wires and dots.

Specimen preparation is of course a crucial point of our work.

We have just switched to PIPS (we used to make our samples using the Gatan
Duo Mill), and we are getting controversial results about the damage
introduced by the milling procedure.

We are perfectly aware of all the differences between the two instruments,
especially the absence of cooling stage and the higher beam current.

Has anyone performed a systematic and careful analysis to try to evaluate
the milling damage on similar materials systems. We are willing to start a
systematic work to assess this issue, but would like to know if anyone has
already done anything on this topic. Also, any collaboration will be more
than welcome.

Thanks.

Massimo




Dr. Massimo Catalano
CNR-IME
Campus Universitario
Via Arnesano
73100 Lecce - ITALY
tel: + 39 0832 322362
fax: + 39 0832 325299
email: massimo.catalano-at-ime.le.cnr.it
http://www.ime.le.cnr.it
http://www.ime.le.cnr.it/sime/sime.htm


{



From daemon Thu Feb 10 19:06:02 2000



From: Roger Moretz :      rcmoretz-at-excite.com
Date: Thu, 10 Feb 2000 09:42:05 -0800 (PST)
Subject: Re: FYI: Congress to allow email charges

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Well, this urban legend has finally made it to the microscopy listserver!
And that is just what it is--a legend, a myth, a fable. As a stamp
collector and one who keeps abreast of all of this kind of stuff, this urban
legend has been debunked in both Linn's Stamp News and Stamp Collector.

All of us have important issues to consider. Please, put this one to rest.

Roger Moretz


On Wed, 9 Feb 2000 21:58:18 -0700, Christina bennett wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I know this is off topic however a change like this could make this type
of
} forum impossible.
}
}
}
}
}
} } Date: Tue, 8 Feb 2000 08:13:53 -0700
} } X-Sender: liperez-at-cnmailsvr.nmsu.edu
} } Mime-Version: 1.0
} } To: all-at-biology.nmsu.edu
} } From: liperez-at-NMSU.Edu (LSPSaldana)
} } Subject: Congress to allow email charges
} } Sender: owner-all-at-biology.nmsu.edu
} } Precedence: bulk
} } X-Keywords:
} } X-UID: 43
} } Status: O
} }
} }
} } } } Congress to allow email charges
} } } }
} } } } Please pass this on to all you know since many of us use e-mail for
} } } } business and to keep up with friends and family, I thought you'd like
} } } } to know the following.
} } } }
} } } } Please jump on it right away and forward this to others.
} } } }
} } } } CNN has reported that within the next two weeks Congress is going to
} } } } vote on allowing telephone companies to CHARGE A TOLL FEE for
} } } } internet access.
} } } } Translation: Every time we send long distance e-mail we will receive
a
} } } } long distance charge. This will get costly. Please visit the
following web
} } } } site and file a complaint. Complain to your Congressperson. We can't
} } } } allow this to pass. The following address will allow you to send an
} } } } e-mail on this subject DIRECTLY to your Congressperson.
} } } }
} } } } http://www.house.gov/writerep {http://www.house.gov/writerep}
} } } }
} } } } Pass this on to your friends. It is urgent! I hope all of you will
pass
} } } } this on to all your friends and family. We should ALL have
} } } } an interest in this one.
} } } }
} } } } WAIT, THERE'S MORE. IN ADDITION, The last few months have revealed an
} } } } alarming trend in the Government of the United States attempting to
} } } } quietly push through legislation that will affect your use of the
} } } Internet.
} } } } Under
} } } } proposed legislation the U.S. Postal Service will be attempting to
bill
} } } } email users out of "alternate postage fees". Bill 602P will permit
the
} } } } Federal Govt. to charge a 5 cent surcharge on every email delivered,
by
} } } } billing Internet Service Providers at source. The consumer would then
be
} } } } billed in turn by the ISP. Washington D.C. lawyer Richard Stepp is
} } } } working without pay to prevent this legislation from becoming law.
The
} } } U.S.
} } } } Postal Service is claiming that lost revenue due to the proliferation
of
} } } } email
} } } } is costing nearly $230,000,000 in revenue per year. You may have
noticed
} } } } their recent ad campaign "There is nothing like a letter".
} } } } Since the average citizen received about 10 pieces of email per day
in
} } } } 1998,
} } } } the cost to the typical individual would be an additional 50 cents
per
} } } } day, or over $180 dollars Per year, above and beyond there regular
} } } Internet
} } } } costs.
} } } } Note that this would be money paid directly to the U.S. Postal
Service
} } } } for a service they do not even provide. The whole point of the
Internet is
} } } } democracy and non-interference. If the federal government is
permitted
} } } } to tamper with our liberties by adding a surcharge to email, who
knows
} } } Where
} } } } it will end. You are already paying an exorbitant price for snail
mail
} } } } because of bureaucratic inefficiency. It currently takes up to 6 days
for
} } } a
} } } } letter to be delivered from New York to Buffalo. If The U.S. Postal
} } } Service
} } } } is
} } } } allowed to tinker with email; it will mark the end of the "free"
} } } } Internet in the United States.
} } } } One congressman, Tony Schnell has even suggested a "twenty to forty
} } } } dollar per month surcharge on all internet service" above and beyond
the
} } } } government's proposed email Charges. Note that most of the major
} } } } newspapers have ignored the story, the only exception being the
} } } } Washingtonian
} } } } which called the idea of email surcharge "a useful concept who's time
has
} } } } come"
} } } } (March 6, 1999) Editorial.
} } } }
} } } } Don't sit by and watch your freedoms erode away! Send this e-mail to
} } } } EVERYONE on your list, and tell all your friends and relatives to
write
} } } } to their congressman and say "No!" to Bill 602P.
} } } }
} } } }
} } } } It will only take a few moments of your time, and could very well be
} } } } instrumental in killing a bill we don't want.
} } } }
} } } } PASS THIS ON TO EVERYONE YOU KNOW WHO USES EMAIL REMEMBER THESE ARE
TWO
} } } } SEPARATE ISSUES THAT EFFECT ALL OF US ONLINE. LET YOU VOICE BE HEARD
} } } } NOW, NOT AFTER
} } } }
} } } }
} } } }
} } } }
} } } }
} } } }
} } }
} }
}
}
}


Roger C Moretz, Ph.D.





_______________________________________________________
Get 100% FREE Internet Access powered by Excite
Visit http://freeworld.excite.com



From daemon Thu Feb 10 19:06:11 2000



From: hank adams :      hpadams-at-bcm.tmc.edu
Date: Thu, 10 Feb 2000 11:45:28 -0600
Subject: GFP and Osmium

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does anyone know if GFP is quenched after OsO4 postfixation?
Hank Adams
Integrated Microscopy Core
Molecular and Cell Biology
Baylor College of Medicine
Houston, TX 77030



From daemon Thu Feb 10 19:06:16 2000



From: Carlos Kazuo Inoki :      kazuo-at-csc.albany.edu
Date: Thu, 10 Feb 2000 13:05:16 -0500 (EST)
Subject: TEM: sample preparation for InSb

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

I'm working with a InSb sample prepared with tripod polishing.
Since the layer is quite thick, more than 3 micron plus a 2 micron buffer
layer, I am experimenting some difficult to prepare a good sample for
cross section observation. The final polishing is done in a Gatan ion
milling set using a low angle. The problem with my samples is how to get a
uniform thickness from the top of the sample to the substrate. Usually the
top of the sample is milled much faster than near to the substrate. I
can't mechanically polish the sample too much, since the sample is
somewhat fragile (brittle). Even in old papers people report this kind of
problem when preparing InSb samples for TEM using ion milling. Does
someone else suffered from this kind of problem: the top part of a
sample being milled away? Oh yes, when I polish the sample I try to make
wedge perpendicular to the sample surface, so the thickness of the sample
is the same from the InSb layer to the substrate after the mechanical
polishing. There is some correlation between the ion milling angle/energy
with this effect? The Gatan epoxy I use to glue a Si piece on top of the
sample should have some influence (charging)? Thanks.

Kazuo

o-------------------------------------------------------o
| Carlos Kazuo Inoki |
| Department of Physics - University at Albany |
| 1400 Washington Ave.- Albany - NY - 12222 |
o-------------------------------------------------------o



From daemon Thu Feb 10 19:06:25 2000



From: Walck. Scott D. :      walck-at-ppg.com
Date: Thu, 10 Feb 2000 13:51:03 -0500
Subject: RE: Be X-ray peaks, solid state physics, how about 2nd row elemen

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I think that I know understand the reason why you get Be X-ray and why
there are energy shifts when it is in different compounds. My next question
then is do the elements between B and F have measurable energy shifts
depending on what material they are in? The key word here is measurable.
The Ka1 and Ka2 peaks are due to transitions form the 2p1/2 and 2p3/2 (LII
and LIII) to the 1s1/2 state. But these would be valence/conductance bands
for the pure elements. (Assuming you collect your X-rays from solid N2, O2,
or F2) The band structure would be different for different materials. For
example, do you see any shifts between B2O3? and BN or C(diamond) and
C(graphite) or TiC? Any microprobers following this thread?

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)



} -----Original Message-----
} From: Hans Dijkstra [mailto:hans.dijkstra-at-edax.nl]
} Sent: Thursday, February 10, 2000 11:01 AM
} To: Walck. Scott D.; 'microprobe'
} Cc: 'Microscopy'
} Subject: RE: Be X-ray peaks -I don't think so
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} ---------.
}
}
} Dear Scott,
}
} A poster does not mean anything. I have seen them with the
} Lithium-Ka energy
} listed, although quantum physics does forbid this X-ray line.
} But I can
} assure you the Be-K line does exist.
}
} On modern EDX systems Be can be detected, provided you have either a
} windowless detector or a detector with a modern polymer type
} window (SUTW,
} SATW, Norvar, or whatever the EDX supplier calls it. My
} apologies if I break
} any trade-marks here...).
} On most demonstration setups your EDS specialist will be
} pleased to show you
} a Be peak. But probably from a pure Be sample only. The absorption
} coefficient (MAC) of Be in basically any matrix is so huge,
} and the X-ray
} fluorescence yield so small, that in any compound with less
} than 50 atomic
} percent Be you will not detect a visible Be peak. For this
} reason most EDX
} installations "in the field" are not setup to detect Be-K radiation.
}
} WDS has a better P/B ratio, so if you have the proper
} multilayer crystal you
} can get results. But peak shifts and peak shape alterations will make
} quantification extremely difficult, not to mention the fact
} that for most
} MACs of Be-K in any matrix we only have a ball-park figure.
}
} Other techniques, like PEELS, are much more suitable for Li
} and Be analysis.
} Best regards,
}
} Hans Dijkstra
}
} Disclaimer: This is my opinion, and not necessarily the one
} from EDAX Inc.
} -------------------------------------------------------------
} EDAX Europe www.edax.com
} Ringbaan Noord 103 Tel.: +31-13-5364000
} P.O. Box 4144 Fax.: +31-13-5356279
} 5004 JC Tilburg E-mail: hans.dijkstra-at-edax.nl
} the Netherlands
} -------------------------------------------------------------
}
}
} } -----Original Message-----
} } From: Walck. Scott D. [mailto:walck-at-ppg.com]
} } Sent: Tuesday, February 08, 2000 6:57 PM
} } To: 'microprobe'; 'harry.ekstrom-at-honeywell.com'
} } Cc: 'Microscopy'
} } Subject: RE: Be X-ray peaks -I don't think so
} }
} }
} }
} --------------------------------------------------------------
} ----------
} } The Microscopy ListServer -- Sponsor: The Microscopy
} Society of America
} } To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} } On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.} html
} }
}
} --------------------------------------------------------------
} ---------.
} }
} }
} } I'm wrong. I guess I should have looked up at the X-ray
} periodic table
} } above my head and saw the 0.108 keV value for Be. The
} reason that I was
} } wrong was that I did not consider the solid state aspects.
} I am still
} right
} } about not having a 2s1/2 to a 1s1/2 transition and that an
} X-ray carries
} } away 1h-bar of angular momentum. What I didn't do was
} think about the
} band
} } structure of a solid. If you have the solid, the 2p bands
} of Be most be
} } spilling into or be the conduction band for the electrons.
} That must be
} the
} } source for the electronic transition. I guess I should
} have engaged brain
} } before typing.
} } My most humble apologies.
} } -Scott
} }
} } Scott D. Walck, Ph.D.
} } PPG Industries, Inc.
} } Glass Technology Center
} } Guys Run Rd. (packages)
} } P. O. Box 11472 (letters)
} } Pittsburgh, PA 15238-0472
} }
} } Walck-at-PPG.com
} }
} } (412) 820-8651 (office)
} } (412) 820-8161 (fax)
} }
} }
} } "The opinions expressed are those of Scott D. Walck and not of PPG
} } Industries, Inc. nor of any PPG-associated companies."
} } --
} }
}
}



From daemon Thu Feb 10 19:06:27 2000



From: Walck. Scott D. :      walck-at-ppg.com
Date: Thu, 10 Feb 2000 13:57:08 -0500
Subject: RE: sample preparation for InSb

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I did InSb and AlInSb by the small angle cleavage technique when I was at
Wright Patterson AFB. I assume since you are using the Tripod Technique
that you require site-specific results. If not, try it. It works really
well on this material.

I am getting to sound like a broken record on this topic, aren't I? See my
post yesterday.

-Scott



Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)


"The opinions expressed are those of Scott D. Walck and not of PPG
Industries, Inc. nor of any PPG-associated companies."
--



Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)



} -----Original Message-----
} From: Carlos Kazuo Inoki [mailto:kazuo-at-csc.albany.edu]
} Sent: Thursday, February 10, 2000 1:05 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: TEM: sample preparation for InSb
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.} html
}
}
} --------------------------------------------------------------
} ---------.
}
}
} Hi,
}
} I'm working with a InSb sample prepared with tripod polishing.
} Since the layer is quite thick, more than 3 micron plus a 2
} micron buffer
} layer, I am experimenting some difficult to prepare a good sample for
} cross section observation. The final polishing is done in a Gatan ion
} milling set using a low angle. The problem with my samples is
} how to get a
} uniform thickness from the top of the sample to the
} substrate. Usually the
} top of the sample is milled much faster than near to the substrate. I
} can't mechanically polish the sample too much, since the sample is
} somewhat fragile (brittle). Even in old papers people report
} this kind of
} problem when preparing InSb samples for TEM using ion milling. Does
} someone else suffered from this kind of problem: the top part of a
} sample being milled away? Oh yes, when I polish the sample I
} try to make
} wedge perpendicular to the sample surface, so the thickness
} of the sample
} is the same from the InSb layer to the substrate after the mechanical
} polishing. There is some correlation between the ion milling
} angle/energy
} with this effect? The Gatan epoxy I use to glue a Si piece on
} top of the
} sample should have some influence (charging)? Thanks.
}
} Kazuo
}
} o-------------------------------------------------------o
} | Carlos Kazuo Inoki |
} | Department of Physics - University at Albany |
} | 1400 Washington Ave.- Albany - NY - 12222 |
} o-------------------------------------------------------o
}
}



From daemon Thu Feb 10 19:06:30 2000



From: David_Bell-at-Millipore.com
Date: Thu, 10 Feb 2000 14:06:06 -0500
Subject: My apologies

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Hello again,

My apologies to the list and especially those at RJ Lee! Hope nobody was
offended.

David Bell
Scientist
Electron Microscopy Lab
Millipore Corporation
80 Ashby Road
Bedford, MA 01730
(781) 533-2108




From daemon Thu Feb 10 19:06:33 2000



From: Damian :      dneuberger-at-mindspring.com
Date: Thu, 10 Feb 2000 13:16:34 -0600
Subject: Re:FYI: Congress to allow email charges -Hoax?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Woody,

I hate to add to the bandwidth clutter but these are very old hoaxes.

Damian


} Is this yet another hoax eating time & bandwidth by being posted without
} confirmation (like the last 3-4 times I saw this message in the pas year or
} two), or is it real?



From daemon Thu Feb 10 19:06:51 2000



From: Alexander Mironov Jr. :      amironov-at-cmns.mnegri.it
Date: Thu, 10 Feb 2000 21:54:09 +0100 (MET)
Subject: Re: GFP and Osmium

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Hank,

Yes, after OsO4 GFP is quenched, moreover it is quenched significantly
(three times or more) even with 0.05% of glutaraldehyde (1Ž0 min.


Dr. Alexander A. Mironov Jr., MD, PhD
Unit of Morphology
Dept. of Cell Biology and Oncology
Consorzio Mario Negri Sud
Via Nazionale, S.Maria Imbaro (Ch)
66030 Italy

Tel. 0039-0872-570-332
Fax 0039-0872-570-412
E-mail: amironov-at-cmns.mnegri.it


On Thu, 10 Feb 2000, hank adams wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Does anyone know if GFP is quenched after OsO4 postfixation?
} Hank Adams
} Integrated Microscopy Core
} Molecular and Cell Biology
} Baylor College of Medicine
} Houston, TX 77030
}
}
}



From daemon Fri Feb 11 18:28:57 2000



From: Fagerland,Jane :      jane.a.fagerland-at-abbott.com
Date: Thu, 10 Feb 2000 15:13:19 -0600
Subject: Summer Internships at Abbott - more information

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thanks to all of you who have shown an interest in our internships. I'd like
to provide a few more details and answer some questions I've received so far
about the summer internships at Abbott.

First: Are the internships open to international students? Technically,
yes. However, interns are employees of Abbott Laboratories during their time
here. Thus, the logistics of processing the necessary paperwork for non-US
citizens for a 12-week employment period would not be practical. So, in
truth, the answer has to be that, except in highly unusual circumstances, we
will limit applications to students in the United States.

Second: Are the internships intended for college or high school students?
The internships are for college students working towards a Bachelor's degree
or higher. Students in the Associate degree programs at Madison Area
Technical College and San Joaquin Delta College are eligible, but priority
will be given to students who already have a Bachelor's degree.

Third: How to apply? Go to the Abbott Laboratories website at abbott.com
and follow the links: Careers, Entry Level Positions, Summer Internship
Program. There you will find an electronic resume form that can be submitted
from your computer. THE DEADLINE FOR SUBMISSION IS MARCH 1.

Along with submitting the electronic resume, please send me either an
e-mailed or faxed copy of your resume, or a hardcopy via snail mail. The
electronic resume ends up in Corporate Staffing, where your skills will be
matched with the internship openings at Abbott. If I have a copy of your
resume, I can make sure that you are considered for positions in the
microscopy department.

If there are any other questions, please feel free to contact me.

Thanks,

Jane A. Fagerland, Ph.D.
Dept. Microscopy and Microanalysis
D-45M/AP31 200 Abbott Park Rd.
Abbott Park IL 60064-6202
voice: (847) 935-0104
fax: (847) 938-5027
e-mail: jane.a.fagerland-at-abbott.com



From daemon Thu Feb 10 19:06:46 2000



From: Robert Underwood :      underwoo-at-u.washington.edu
Date: Thu, 10 Feb 2000 14:04:19 -0800 (PST)
Subject: Re: autofluorescence quenching

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hello from Seattle,

There was a paper by Cowen, Haven and Burnstock 1985, using Pontamine Sky
Blue couterstain to reduce autofluorescence.

Also a paper by Kittleburger, Davis, and Stephans in Acta Histochem 89,
using Eriochrome Black T.

Bob
Morphology Core
U of Washington

On Wed, 9 Feb 2000, Corazon Bucana wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I am working with archival specimens of formalin-fixed paraffin embedded
} tissues and I am getting tremendous autofluorescence. I looked at tissue
} sections after the sections have been de-waxed using either FITC or
} rhodamine filter sets and red cells and connective tissues are brightly
} fluorescent. Is there a way of suppressing the autofluorescence and still
} retain reactivity of tissue to antibodies? I would appreciate any comments
} or suggestions.
}
} *******************************************************
} Corazon D. Bucana, Ph.D.
} Department of Cancer Biology
} U.T. M.D. Anderson Cancer Center
} 1515 Holcombe Blvd. Box 173
} Houston, Texas 77030
} Phone: (713) 792-8106
} FAX: (713) 792-8747
} Email:bucana-at-audumla.mdacc.tmc.edu
} FAX: (713) 792-8747
}
}
}



From daemon Thu Feb 10 19:06:49 2000



From: Diana van Driel :      dianavd-at-eye.usyd.edu.au
Date: Fri, 11 Feb 2000 10:01:58 +1100
Subject: Re: New Gold Enhancement Reagents

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 8:51 AM +0100 4/2/00, Alexander Mironov Jr. wrote:
} I have tried GoldEnhance for preembedding protocol to label intracellular
} structures. It is marvelous. Buy it and use it - you will not be
} unsatisfied. I have no any interest in Nanoprobes, I just like these dense
} gold particles.
} Practically all they claim is true:
} - light insensitive
} - no self-nucleation
} - do not react with buffer ions
} - is not dissolved by uranil and osmium (I have treat for 1 h)

I have been using BioCell's silver enhancement kit for years and
particularly like it for the light insensitivity - one can monitor
the reaction under the light microscope. Is it possible to do this
with the GoldEnhance as well?

GoldEnhance sounds perfect - has anyone had any problems with it?

Diana



Diana van Driel
Department of Clinical Ophthalmology
Sydney University
GPO Box 4337
Sydney NSW
AUSTRALIA 2001

Phone 61 2 938 27278/27395
Mob 0412 165 075
Fax 61 2 938 27318



From daemon Fri Feb 11 18:29:01 2000



From: Paul Webster :      pwebster-at-hei.org
Date: 10 Feb 00 20:26:38 -0800
Subject: Re:FYI: Congress to allow email charges -Hoax?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Reply to: Re:FYI: Congress to allow email charges -Hoax?
I too had my suspicions. It looks very much like the "Internet Access Tax" letter from a few years ago. See: {http://urbanlegends.miningco.com/culture/urbanlegends/library/weekly/aa010798.htm} for details.

There are many sites documenting this sort of stuff. {http://www.hoaxkill.com/} is an example .

Disclaimer: I have no affiliation to this site.

Regards,
Paul Webster

Paul Webster, Ph.D.
Associate Scientist & Director
Ahmanson Advanced Electron Microscopy & Imaging Center
House Ear Institute
2100 West Third St.
Los Angeles, CA 90057

Phone: (213) 273-8026
Fax: (213) 413-6739
e-mail: pwebster-at-hei.org
http://www.hei.org/htm/aemi.htm



From daemon Fri Feb 11 18:29:06 2000



From: Nelson+ Conti :      NelsonC51-at-excite.com
Date: Thu, 10 Feb 2000 21:07:34 -0800 (PST)
Subject: Re:FYI: Congress to allow email charges -Hoax?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html





On Thu, 10 Feb 2000 13:16:34 -0600, Damian wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Woody,
}
} I hate to add to the bandwidth clutter but these are very old hoaxes.
}
} Damian
}
}
} } Is this yet another hoax eating time & bandwidth by being posted without
} } confirmation (like the last 3-4 times I saw this message in the pas year
or
} } two), or is it real?
}
}
Damian's response is indeed correct -- see posted responses by
"David_Bell-at-millipore.com", "EvexAnalyt-at-aol.com", Scott Wight, Kalman Rubin,
Dr. Gary Gaugler, donald j. marsh, Roger Moretz, Laurie Wallin and Damian.
Being new to the MSA listserver, I'm very relieved that such notices (err,
BANDWIDTH-OCCUPYING, ANNOYING HOAXES) are determined to be false by very
astute readers. I fell for this, and, upon reading other people's notices, I
am upset that someone would suggest spreading such hoaxes around like the
classic junk chain mail activities done at school (or even college). Such
notices clog up the space needed for important issues regarding microscopy.
Anyway .. I'm responding merely to alert other GULLIBLE people (like myself)
not to believe such notices, as well as be thankful that this has been a
recurring event in the MSA listserver.
Nelson Conti





_______________________________________________________
Get 100% FREE Internet Access powered by Excite
Visit http://freeworld.excite.com



From daemon Fri Feb 11 18:29:18 2000



From: Alexander Mironov Jr. :      amironov-at-cmns.mnegri.it
Date: Fri, 11 Feb 2000 10:31:02 +0100 (MET)
Subject: Re: New Gold Enhancement Reagents

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Diana,

IMHO, the main problem with GoldEnhance is some inhomogenity in particle
size but for my purposes it is irrelevant. Also the kit works good in my
hands at pH 7.4 but does not at pH 6.5 (as claimed Nanoprobes).

Sincerely,
Dr. Alexander A. Mironov Jr., MD, PhD
Unit of Morphology
Dept. of Cell Biology and Oncology
Consorzio Mario Negri Sud
Via Nazionale, S.Maria Imbaro (Ch)
66030 Italy

Tel. 0039-0872-570-332
Fax 0039-0872-570-412
E-mail: amironov-at-cmns.mnegri.it





From daemon Fri Feb 11 18:29:19 2000



From: GUIDO LŸšND :      lueoend-at-zzmk.unizh.ch
Date: Fri, 11 Feb 2000 10:31:14 +0100
Subject: TEM Formvar Film Cast on Glass

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear readers,
could anyone give me some hints or tips how I can separate a formvarfilm
(0.5% in chloroform) from the glassslide ? Breathing or freezing doesn't
help at all. It works better with non-washed slides, but then I get an
uneven and dirty surface of the film.
I appreciate every kind of support.
Best regards

Guido Luond
Institute of Oral Microbiology and
General Immunology
Univ. of Zurich
Plattenstr. 11
CH-8028 Zurich, Switzerland
E-mail: lueoend-at-zzmk.unizh.ch



From daemon Fri Feb 11 18:29:21 2000



From: richard.beanland-at-gecm.com
Date: Fri, 11 Feb 2000 09:48:48 +0000 (GMT)
Subject: Re: TEM: Sample preparation for InSb

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Carlos,
sounds like you may be having a problem with differential milling rates. Try milling over a restricted angle; you can use oscillation (if your ion mill is set up to do this) or make C-shaped Ta plate shields which you fix on the sample holding plates of your ion mill, with the sample at the centre of the 'C'. If you put your sample in the plates such that the interface is parallel to the opening of the shield, the average direction of the ion beam will be perpendicular to the interface. This will prevent the ion beam milling parallel to the glue line and stop the layers disappearing before the substrate. If you stick to angles below 10 degrees you will get better results, and of course use liquid nitrogen cooling and/or iodine. My apologies if this isn't very clear - it's hard to describe everything with just words! I can send you a picture of the sample holder I use if you like.

Best regards,

Richard Beanland

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi,
}
} I'm working with a InSb sample prepared with tripod polishing.
} Since the layer is quite thick, more than 3 micron plus a 2 micron buffer
} layer, I am experimenting some difficult to prepare a good sample for
} cross section observation. The final polishing is done in a Gatan ion
} milling set using a low angle. The problem with my samples is how to get a
} uniform thickness from the top of the sample to the substrate. Usually the
} top of the sample is milled much faster than near to the substrate. I
} can't mechanically polish the sample too much, since the sample is
} somewhat fragile (brittle). Even in old papers people report this kind of
} problem when preparing InSb samples for TEM using ion milling. Does
} someone else suffered from this kind of problem: the top part of a
} sample being milled away? Oh yes, when I polish the sample I try to make
} wedge perpendicular to the sample surface, so the thickness of the sample
} is the same from the InSb layer to the substrate after the mechanical
} polishing. There is some correlation between the ion milling angle/energy
} with this effect? The Gatan epoxy I use to glue a Si piece on top of the
} sample should have some influence (charging)? Thanks.
}
} Kazuo
}
} o-------------------------------------------------------o
} | Carlos Kazuo Inoki |
} | Department of Physics - University at Albany |
} | 1400 Washington Ave.- Albany - NY - 12222 |
} o-------------------------------------------------------o
}

==============================================================
Richard Beanland
Caswell Technology,
Caswell,
Towcester,
Northants NN12 8EQ

e-mail richard.beanland-at-gecm.com
Tel. +44 1327 356363
Fax. +44 1327 356398
==============================================================
"The information contained in this message is legally privileged and confidential information intended for the eyes of the individual or entity named above. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message is strictly prohibited. If you have received this message in error, please notify us immediately by telephone.
Caswell Technology is the trading name of Marconi Caswell Limited. Registered in London No. 3694360 Registered Office: One Bruton Street London W1X 8AQ. Holding Company: Marconi plc."






From daemon Fri Feb 11 18:29:29 2000



From: Barbara Foster :      mme-at-map.com
Date: Fri, 11 Feb 2000 07:50:18 -0500
Subject: Re: Color vs. monochrome cameras - a different approach

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


David,

Is your end goal hi res imaging or ratioing? If the latter, I saw an
interesting technology at Cell Bio which might be of help: the PARISS
Spectral imaging system from LightForm. I saw very early versions of this
technology some years ago at PITTCON but it has really matured and offers
an interesting alternative. PARISS can acquire 240 spectra simultaneously,
full field or in a region of interest. It really crashes through the old
barriers imposed by filter cubes. Also, despite the radical differences in
intensity, it can acquire the transmitted light image simultaneously with
the fluorescence image and present the combined results with perfect
registration. The system retrofits to existing microscopes and is
moderately priced, considering all the accessories (2 cameras,
spectrometer, motorized stage, software). If you are interested, visit
their website at lightforminc.com or contact Jeremy Lerner (908-281-9098).

Caveat: MME has no financial interest in this product.

Hope this is helpful.

Best regards,
Barbara Foster
Consortium President
Microscopy/Microscopy Education ...Educating microscopists for greater
productivity.

125 Paridon Street Suite 102 Springfield, MA 01118
PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com
Visit our web site {http://www.MME-Microscopy.com/education}



At 10:23 AM 2/10/00 -0500, David Knecht wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Fri Feb 11 18:29:30 2000



From: L. D. Marks :      ldm-at-apollo.numis.nwu.edu
Date: Fri, 11 Feb 2000 07:35:08 -0600 (CST )
Subject: Re: coherence and spot size

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Sorry, I think a small correction/clarification is requires. This is ONLY
for an incoherently filled condensor aperture, i.e. a large beam with C2
fully focussed. This approximation is not true with the newer microscopes!

On Thu, 10 Feb 2000, Jonathan Barnard wrote:

} Born & Wolf (section 10.4.2)
} "Hence if the linear dimensions of the source and the distance between P1
} and P2 (points in the imaging plane) are small compared to the distance of
} these points from the source, the degree of coherence, |mu_12| is equal to
} the absolute value of the normalized Fourier transform of the intensity
} function of the source"
}

++++++++++++++++++++++++++++++++++++++++++++++++
Laurence Marks
Department of Materials Science and Engineering
Northwestern University
fax: (847) 491-7820
mailto:l-marks-at-nwu.edu
http://www.numis.nwu.edu
++++++++++++++++++++++++++++++++++++++++++++++++



From daemon Fri Feb 11 18:29:38 2000



From: Hans Dijkstra :      hans.dijkstra-at-edax.nl
Date: Fri, 11 Feb 2000 14:51:03 +0100
Subject: RE: Be X-ray peaks, solid state physics, how about 2nd row elements

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Scott,

WDS spectra of Boron, Carbon, Nitrogen and Oxygen in many compounds have
been extensively recorded by Bastin and Heijligers in the 1980's and early
1990's. They report that peak shifts and peak shape alterations are clearly
present in Boron compounds. In the case of TiB crystals it was even found
that the crystal orientation influences the peak shape and position, i.e. if
you rotate the sample 90 degrees you get a different peak shape. For Carbon
the peak shapes are practically unaffected by the bonding with other
elements.

Please check "Quantitative Electron Probe Microanalysis of Boron in Binary
Borides" by Bastin and Heijligers, University of Technology Eindhoven, the
Netherlands, 1997, ISBN 90-3860-898-5.

In EDX the peak shifts are undetectable. Modern EDX systems give a FWHM of
around 60-65 eV for these very light elements, so a peak shift of a few eV
is basically undetectable. For many users this makes EDX the prefered
technique to quantify Boron compounds, since with WDS you need to record
either the full peak (tedious) or use fixed area-peak-fraction correction
methods. In EDX we can simple ignore peak shape and peak position changes.

Best regards,

Hans Dijkstra

Disclaimer: This is my opinion, and not necessarily the one from EDAX Inc.
-------------------------------------------------------------
EDAX Europe www.edax.com
Ringbaan Noord 103 Tel.: +31-13-5364000
P.O. Box 4144 Fax.: +31-13-5356279
5004 JC Tilburg E-mail: hans.dijkstra-at-edax.nl
the Netherlands
-------------------------------------------------------------

} -----Original Message-----
} From: Walck. Scott D. [mailto:walck-at-ppg.com]
} Sent: Thursday, February 10, 2000 7:51 PM
} To: 'Microscopy'
} Subject: RE: Be X-ray peaks, solid state physics, how about 2nd row
} elements
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I think that I know understand the reason why you get Be X-ray and why
} there are energy shifts when it is in different compounds. My next
question
} then is do the elements between B and F have measurable energy shifts
} depending on what material they are in? The key word here is measurable.
} The Ka1 and Ka2 peaks are due to transitions form the 2p1/2 and 2p3/2 (LII
} and LIII) to the 1s1/2 state. But these would be valence/conductance
bands
} for the pure elements. (Assuming you collect your X-rays from solid N2,
O2,
} or F2) The band structure would be different for different materials. For
} example, do you see any shifts between B2O3? and BN or C(diamond) and
} C(graphite) or TiC? Any microprobers following this thread?
}
} -Scott
}
} Scott D. Walck, Ph.D.
} PPG Industries, Inc.
} Glass Technology Center
} Guys Run Rd. (packages)
} P. O. Box 11472 (letters)
} Pittsburgh, PA 15238-0472
}
} Walck-at-PPG.com
}
} (412) 820-8651 (office)
} (412) 820-8161 (fax)
}



From daemon Fri Feb 11 18:29:33 2000



From: Massimo Catalano :      massimo.catalano-at-ime.le.cnr.it
Date: Fri, 11 Feb 2000 15:08:23 +0100
Subject: PIPS and milling damage. Second message.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listservers:

first of all I would like to thank of the people who spent part of their
time trying to share their knowledge with me, and also to the manufacturers
who of course replied my messages.

All messages contained very important suggestions, that I need to study and
evaluate, and I will contact personally the people that offered to share
their previous experience and/or to collaborate.

Our work on InGaAs/GaAs quantum wells, single and stacked quantum wires,
single and stacked quantum dots, consists of conventional TEM, high
resolution transmission electron microscopy and high spatial resolution
analytical electron microscopy, that we perform in close collaboration with
Arizona State University, using a FEG/STEM machine equipped with EELS and
EDX. One of the goals of the research is to evaluate compositional
fluctuations in the well and in the dots at nanoscale.

As everybody knows, the requirements from a TEM sample for imaging are
completely different from the requirements necessary to perform high
spatial resolution analytical work, were effects such as amorphisazion,
surface contamination are critical.

We have a pretty well estabilished specimen preparation technique, but
before publishing results into the scientific community, we want to be sure
that what we observe is what is in the sample (isn't this the main concern
of every microscopist?).

That's why all suggestions, opinions and critics are strongly appreciated.

Best regards

Massimo


Dr. Massimo Catalano
CNR-IME
Campus Universitario
Via Arnesano
73100 Lecce - ITALY
tel: + 39 0832 322362
fax: + 39 0832 325299
email: massimo.catalano-at-ime.le.cnr.it
http://www.ime.le.cnr.it
http://www.ime.le.cnr.it/sime/sime.htm




From daemon Fri Feb 11 18:29:35 2000



From: Tamara Howard :      howard-at-cshl.org
Date: Fri, 11 Feb 2000 09:13:34 -0500 (EST)
Subject: Re: TEM Formvar Film Cast on Glass

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I don't wash the slides, just wipe the dry slide with a Kim-Wipe a few
times. Works like a charm, whatever the weather! (Very grey and nasty here
on "the island" today, by the way).

Tamara Howard
CSHL


On Fri, 11 Feb 2000, GUIDO L[ISO-8859-1] üöND wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear readers,
} could anyone give me some hints or tips how I can separate a formvarfilm
} (0.5% in chloroform) from the glassslide ? Breathing or freezing doesn't
} help at all. It works better with non-washed slides, but then I get an
} uneven and dirty surface of the film.
} I appreciate every kind of support.
} Best regards
}
} Guido Luond
} Institute of Oral Microbiology and
} General Immunology
} Univ. of Zurich
} Plattenstr. 11
} CH-8028 Zurich, Switzerland
} E-mail: lueoend-at-zzmk.unizh.ch
}
}
}



From daemon Fri Feb 11 18:29:36 2000



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Fri, 11 Feb 2000 08:27:09 -0600
Subject: Re: TEM: Formvar Film Cast on Glass

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Guido,

There is a product called "Victawet", which is available here in the U.S.
from various EM suppliers. It comes in a small vial that lasts forever and
is an off-white, waxy or soapy substance. We used to take our slides and
clean them very well with lint-free papers, then place them in a rack inside
a vacuum evaporator, with the side intended for the Formvar film facing a
tungsten basket connected to the electrodes. Then we place a piece of
Victawet about the size of a grain of rice in the basket, pump down the
system to high vacuum, and slowly heat the tungsten basket until it glows
dull red. (If you increase the current too quickly the little piece will
jump out of the basket.) The slides will begin to look fogged, as if
someone had breathed moisture onto them. You can stop at that point---you
only need a little.

Store these coated slides until needed, then take them as required and
polish them very carefully with lint-free paper. They should feel slippery.
Use these cleaned slides to dip into the Formvar solution and the film
should separate easily.

Please note that the type of slide and the humidity levels in the workplace
also have a large effect. I have worked in places where films would
separate from any slide, every day, with no Victawet coating. I have also
worked in places where we could only get Formvar films on occasion (who
knows why?), and then we made a bunch of them at once.

Hope this helps.

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
(573) 882-8304
(573) 884-5414 (fax)
email: tindallr-at-missouri.edu {mailto:tindallr-at-missouri.edu}
http://biotech.missouri.edu/emc {mailto:tindallr-at-missouri.edu}



Dear readers,

could anyone give me some hints or tips how I can separate a formvarfilm

(0.5% in chloroform) from the glassslide ? Breathing or freezing doesn't

help at all. It works better with non-washed slides, but then I get an

uneven and dirty surface of the film.

I appreciate every kind of support.

Best regards

Guido Luond

Institute of Oral Microbiology and

General Immunology

Univ. of Zurich

Plattenstr. 11

CH-8028 Zurich, Switzerland

E-mail: lueoend-at-zzmk.unizh.ch


Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
(573) 882-8304
(573) 884-5414 (fax)
email: tindallr-at-missouri.edu {mailto:tindallr-at-missouri.edu}
http://biotech.missouri.edu/emc {mailto:tindallr-at-missouri.edu}




From daemon Fri Feb 11 18:29:40 2000



From: mike.santanajr-at-amd.com
Date: Fri, 11 Feb 2000 08:48:14 -0600
Subject: RE: FYI: Congress to allow email charges -Hoax? (STOP)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greetings,

Hasn't anyone realized that the "hoax" email is a success even as a
failure. Everyone is responding so much about the email being a hoax that
we're using up band width and cluttering email accounts. We all know it's a
hoax..., let's leave it at that.....


Regards,

Mike Santana, Jr.
Fab25 Product Engineering
Advanced Micro Devices
Desk - (512) 602-4172
Pager - (512) 622-2494
email - mike.santanajr-at-amd.com

On Thursday, February 10, 2000 10:27 PM, Paul Webster
[SMTP:pwebster-at-hei.org] wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Reply to: Re:FYI: Congress to allow email charges -Hoax?
} I too had my suspicions. It looks very much like the "Internet Access
Tax" letter from a few years ago.
See: {http://urbanlegends.miningco.com/culture/urbanlegends/library/weekly/aa
010798.htm} for details.
}
} There are many sites documenting this sort of stuff.
{http://www.hoaxkill.com/} is an example .
}
} Disclaimer: I have no affiliation to this site.
}
} Regards,
} Paul Webster
}
} Paul Webster, Ph.D.
} Associate Scientist & Director
} Ahmanson Advanced Electron Microscopy & Imaging Center
} House Ear Institute
} 2100 West Third St.
} Los Angeles, CA 90057
}
} Phone: (213) 273-8026
} Fax: (213) 413-6739
} e-mail: pwebster-at-hei.org
} http://www.hei.org/htm/aemi.htm
}



From daemon Fri Feb 11 18:29:48 2000



From: Jesœs Ricote :      jricote-at-icmm.csic.es
Date: Fri, 11 Feb 2000 16:21:51 +0100
Subject: ion thinner: users of Bal-Tec RES 010

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear listmembers,

I would like to contact users of Bal-Tec RES 010 ion thinner to comment on
performance of this equipment. We have found over the years frequent
stability problems of the guns, which are not solved only with cleaning. We
are at the moment checking any possible source of unstability. We would
like advise from anyone who has had similar problems with this apparatus,
to know if we are missing something and what would be the best course of
action.

Thanks in advance

Jesœs Ricote
-------------------------------------------------------------
Dr. Jesœs Ricote
Departamento de Materiales FerroelŽctricos
Instituto de Ciencia de Materiales de Madrid
Consejo Superior de Investigaciones Cientificas (CSIC)
Cantoblanco
28049 Madrid
SPAIN

Phone: ( + 34) 91 334 90 00 (Ext. 268)
Fax: ( + 34) 91 372 06 23
e-mail: jricote-at-icmm.csic.es
http://www.icmm.csic.es/mf/ferroesp.htm
-------------------------------------------------------------



From daemon Fri Feb 11 18:29:47 2000



From: Massimo Catalano :      massimo.catalano-at-ime.le.cnr.it
Date: Fri, 11 Feb 2000 16:22:30 +0100
Subject: PIPS and milling damage: Second message

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listservers:

first of all I would like to thank of the people who spent part of their
time trying to share their knowledge with me, and also to the manufacturers
who of course replied my messages.

All messages contained very important suggestions, that I need to study and
evaluate, and I will contact personally the people that offered to share
their previous experience and/or to collaborate.

Our work on InGaAs/GaAs quantum wells, single and stacked quantum wires,
single and stacked quantum dots, consists of conventional TEM, high
resolution transmission electron microscopy and high spatial resolution
analytical electron microscopy, that we perform in close collaboration with
Arizona State University, using a FEG/STEM machine equipped with EELS and
EDX. One of the goals of the research is to evaluate compositional
fluctuations in the well and in the dots at nanoscale.

As everybody knows, the requirements from a TEM sample for imaging are
completely different from the requirements necessary to perform high
spatial resolution analytical work, were effects such as amorphisazion,
surface contamination are critical.

We have a pretty well estabilished specimen preparation technique, but
before publishing results into the scientific community, we want to be sure
that what we observe is what is in the sample (isn't this the main concern
of every microscopist?).

That's why all suggestions, opinions and critics are strongly appreciated.

Best regards

Massimo


Dr. Massimo Catalano
CNR-IME
Campus Universitario
Via Arnesano
73100 Lecce - ITALY
tel: + 39 0832 322362
fax: + 39 0832 325299
email: massimo.catalano-at-ime.le.cnr.it
http://www.ime.le.cnr.it
http://www.ime.le.cnr.it/sime/sime.htm




From daemon Fri Feb 11 18:29:51 2000



From: richard.beanland-at-gecm.com
Date: Fri, 11 Feb 2000 15:56:43 +0000 (GMT)
Subject: Re: U-shaped Ta for IBT

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ah. Bit of a problem, that. I didn't mention how far away the shield is from the sample - about 3 or 4 mm - and that for low angles I use a wedge-shaped shield to deflect ions away from the specimen, rather than back on to it. Did you really try it that quickly? I only sent the e-mail a few hours ago! I did expect anyone who was going to have a go to ask me for a picture of my holder.
As to cleaning your sample, I think you can only ion mill it some more, with amended shields...

Richard


} Dear Richard,
}
} I did as you suggested in my sample preparation. However, the Ta was
} sputtered on the surface of the
} specimen. Could you suggest me how to avoid or how to clean the Ta on the
} specimen surface.
}
} Chengge Jiao
} H.H.Wills Physics Lab
} University of Bristol, U.K
} c.g.jiao-at-bristol.ac.uk

==============================================================
Richard Beanland
Caswell Technology,
Caswell,
Towcester,
Northants NN12 8EQ

e-mail richard.beanland-at-gecm.com
Tel. +44 1327 356363
Fax. +44 1327 356398
==============================================================
"The information contained in this message is legally privileged and confidential information intended for the eyes of the individual or entity named above. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message is strictly prohibited. If you have received this message in error, please notify us immediately by telephone.
Caswell Technology is the trading name of Marconi Caswell Limited. Registered in London No. 3694360 Registered Office: One Bruton Street London W1X 8AQ. Holding Company: Marconi plc."






From daemon Fri Feb 11 18:29:52 2000



From: Walck. Scott D. :      walck-at-ppg.com
Date: Fri, 11 Feb 2000 11:16:35 -0500
Subject: RE: PIPS and milling damage: Second message

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


One of the benefits from the small angle cleavage technique on the III-V
compounds is that often a "step-like" sample is produced. When this
happens, you have a perfect sample for both High resolution and analytical
work. In the very thin steps, you can do HREM and EELS. In the thicker
steps, you can do EDS analysis.
-Scott


Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)



} -----Original Message-----
} From: Massimo Catalano [mailto:massimo.catalano-at-ime.le.cnr.it]
} Sent: Friday, February 11, 2000 10:23 AM
} To: Microscopy-at-sparc5.Microscopy.Com
} Subject: PIPS and milling damage: Second message
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} ---------.
}
}
} Dear Listservers:
}
} first of all I would like to thank of the people who spent
} part of their
} time trying to share their knowledge with me, and also to the
} manufacturers
} who of course replied my messages.
}
} All messages contained very important suggestions, that I
} need to study and
} evaluate, and I will contact personally the people that
} offered to share
} their previous experience and/or to collaborate.
}
} Our work on InGaAs/GaAs quantum wells, single and stacked
} quantum wires,
} single and stacked quantum dots, consists of conventional TEM, high
} resolution transmission electron microscopy and high spatial
} resolution
} analytical electron microscopy, that we perform in close
} collaboration with
} Arizona State University, using a FEG/STEM machine equipped
} with EELS and
} EDX. One of the goals of the research is to evaluate compositional
} fluctuations in the well and in the dots at nanoscale.
}
} As everybody knows, the requirements from a TEM sample for
} imaging are
} completely different from the requirements necessary to perform high
} spatial resolution analytical work, were effects such as
} amorphisazion,
} surface contamination are critical.
}
} We have a pretty well estabilished specimen preparation
} technique, but
} before publishing results into the scientific community, we
} want to be sure
} that what we observe is what is in the sample (isn't this the
} main concern
} of every microscopist?).
}
} That's why all suggestions, opinions and critics are strongly
} appreciated.
}
} Best regards
}
} Massimo
}
}
} Dr. Massimo Catalano
} CNR-IME
} Campus Universitario
} Via Arnesano
} 73100 Lecce - ITALY
} tel: + 39 0832 322362
} fax: + 39 0832 325299
} email: massimo.catalano-at-ime.le.cnr.it
} http://www.ime.le.cnr.it
} http://www.ime.le.cnr.it/sime/sime.htm
}
}
}



From daemon Fri Feb 11 18:29:56 2000



From: Heather A Owen :      owenha-at-csd.uwm.edu
Date: Fri, 11 Feb 2000 11:20:25 -0600 (CST)
Subject: Re: TEM Formvar Film Cast on Glass

Contents Retrieved from Microscopy Listserver Archives
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I've always had good luck getting the film off by polishing the slide
first with Ross Optical Lens Tissue. My major professor taught me this,
and his explaination was that it has silicone in it. Once you have cast
the film onto the slide and allowed it to dry, cut through the film at the
edges and bottom of the slide by running a different slide along them.
Then breathe on the film and lower it into the water dish.

Good luck,
Heather Owen

p.s. I have no affiliation with the company that makes, or any of the EM
supply houses that sell Ross lens paper.

Heather A. Owen, Director
Electron Microscope Laboratory
Department of Biological Sciences
University of Wisconsin - Milwaukee
(414)229-6816




From daemon Fri Feb 11 18:29:58 2000



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Fri, 11 Feb 2000 09:41:58 -0800
Subject: RE: Be X-ray peaks, solid state physics, how about 2nd row

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Dear Scott,
In the Microbeam Analysis 1985, G.F. Bastin and H.J.M. Heijligers have the
first article, entitled: "Quantitative Electtron-Probe Microanalysis of Very
Light Elements" (page 1). They have several articles in that and succeeding
years in which they try to analyse every element and compound in the B to F
range material that they can get to sit still under the EPMA beam. They did
a very valuable set of studies that is worth looking into if you want to do
any very light element analysis. They cover peak shifts, peak shape changes,
even crystallographic direction sensitivities.
At 01:51 PM 2/10/00 -0500, you wrote:

}
} I think that I know understand the reason why you get Be X-ray and why
} there are energy shifts when it is in different compounds. My next question
} then is do the elements between B and F have measurable energy shifts
} depending on what material they are in? The key word here is measurable.
} The Ka1 and Ka2 peaks are due to transitions form the 2p1/2 and 2p3/2 (LII
} and LIII) to the 1s1/2 state. But these would be valence/conductance bands
} for the pure elements. (Assuming you collect your X-rays from solid N2, O2,
} or F2) The band structure would be different for different materials. For
} example, do you see any shifts between B2O3? and BN or C(diamond) and
} C(graphite) or TiC? Any microprobers following this thread?
}
} -Scott
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Fri Feb 11 18:30:00 2000



From: Elaine Humphrey :      ech-at-unixg.ubc.ca
Date: Fri, 11 Feb 2000 10:12:05 -0800
Subject: Re: questions for MSA members microwave

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Hello Casey/Marty
I cannot answer for the first question as we use Diotome knives and I have
no experience with the other.

But the answer to the second question is "Yes." At least for us it is. We
are a facility used by the Faculty of Medicine and Science so many and
varied samples come through here for electron, light, and confocal
microscopy. We have been working up some protocols for difficult specimens
such as nematodes, drosophila larvae and plant material. The power supply
allows control so that you don't have to do any other processing to the
specimens than toss them in the fixative. Since the fix and post fix enter
the specimens at low power ( {200 watts) without damaging the specimens
(full power is usually about 800 watts) we can fix in about 45 minutes for
something which would normally be in 24 hours. And we don't have to punch
holes in the specimen.

We have just been playing with the introduction of fluorescent dyes for
light microscopy and confocal in C. elegans, without the use of agents to
distrupt the membranes to get the dye in. So far we have got the timings
for beautiful staining of the tail region and partial staining of the
thicker sections. We still have to get it right but the initial experiments
are very positive.

No-one seems to know why the microwaves work, but the hypothesis is that at
low power, the cell membranes are "jiggled about" to let the fix/dye in
without distrupting them.

We are a very busy lab, and our experiments are constantly getting
interupted so it is taking longer than we'd like to pin down a finished
protocol.

I would be interested in receiving protocols from labs who have already got
it to work for their specimens. We are finding that every specimen is
different and the protcols have to be tweaked for each one.
Elaine

}
} A colleague would like your responses to these questions. I believe that I
} say something on the first question about a year ago and apologize for
} asking again.

} } Here's what I/we need to know.
} }
} } 1) Are Microstar diamond knives equal to Diatome. We have an offer that
} } will save us about $1200 on three knives ( one 3mm ultra with 45 degree
} } angle, one 3mm ultra with 35 degree angle, one 10 mm histo knife).
} }
} }
} } 2) Is the power controller feature on the Pelco Microwave Tissue processor
} } (model #3451) worth it? (costs about $1300 additional compared to the
} } Pelco 3450 without the power controller). I plan to do immunolocalization
} } work, but am not sure this power controller feature is necessary or worth
} } the cost.
} }
} } Casey

Marty Reed


Dr. Elaine Humphrey
Biosciences Electron Microscopy Facility
University of British Columbia
6270 University Blvd, mail-stop Botany
Vancouver, BC
CANADA, V6T 1Z4
Phone: 604-822-3354
FAX: 604-822-6089
e-mail: ech-at-unixg.ubc.ca




From daemon Fri Feb 11 18:30:02 2000



From: Maria.Fazio-Zanakis-at-aventis.com
Date: Fri, 11 Feb 2000 12:17:37 -0600
Subject: TEM Formvar Film Cast on Glass

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Dear Guido,
I have learned long ago not to use films cast on slides. It is easier
and much cleaner to spread your films on the surface of water in a trough.
You can vary the thickness by the amount of drops you use and chose the area
you want to place your grids. This method gave me beautiful and sturdy
films.
Regards,
Maria

Maria Fazio-Zanakis
Bioimaging and Molecular Histology
Aventis Pharmaceuticals
1-908-231-3357
Fax: 1-908-231-3962
Email: Maria.Fazio-Zanakis-at-Aventis.com


-----Original Message-----
} From: GUIDO LŸšND [mailto:lueoend-at-zzmk.unizh.ch]
Sent: Friday, February 11, 2000 4:31 AM
To: EM-Tips


Dear readers,
could anyone give me some hints or tips how I can separate a formvarfilm
(0.5% in chloroform) from the glassslide ? Breathing or freezing doesn't
help at all. It works better with non-washed slides, but then I get an
uneven and dirty surface of the film.
I appreciate every kind of support.
Best regards

Guido Luond
Institute of Oral Microbiology and
General Immunology
Univ. of Zurich
Plattenstr. 11
CH-8028 Zurich, Switzerland
E-mail: lueoend-at-zzmk.unizh.ch



From daemon Fri Feb 11 18:30:06 2000



From: Paul Webster :      pwebster-at-mailhouse.hei.org
Date: 11 Feb 00 10:43:54 -0800
Subject: RE: TEM Formvar Film Cast on Glass

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From: Paul Webster :      pwebster-at-mailhouse.hei.org
Date: 11 Feb 00 10:43:54 -0800
Subject: RE: TEM Formvar Film Cast on Glass

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Reply to: RE: TEM Formvar Film Cast on Glass
Hi Guido,

Here is my recipe which has worked on four continents at altitudes up to 2,000m and in all weathers (humid to dry, cold to hot). Plus, it does not use those very unscientific body fluids I often see being used on this list!

Dissolve the formvar in chloroform as a concentration higher than will make the correct film thickness (film thickness will vary with the speed the glass is drawn out of the solution as well as with formvar concentration).

Take a glass slide (it is important to use precleaned 25x75 mm slides, VWR Scientific, Cat number 48312-002) and dip it in absolute ethanol. Immediately dry it with Ross lens tissue. If you wash it any other way or dry it with anything other than the Ross lens tissue, the film will not come off the glass.

Coat the slide immediately and let the film dry. Score the edges of the film so that it will easily detach from the glass and float it onto the water surface. Evaluate the film thickness and adjust the formvar concentration by adding chloroform to the solution.

I know there are many versions of this method that work. However, this is the only method I have found that is completely oblivious to the weather (still sunny in LA) and has been reproduced by many people.
in my experience, the formvar dissolved in choroform does not seem to have the short shelf life I read about recently. I have used the same solution for years, topping it up with chloroform to keep it at a concentration that will produce thin films.

Regards,

Paul Webster

Paul Webster, Ph.D
House Ear Institute
2100 West Third Street
Los Angeles, CA 90057
phone:213 273 8026
fax: 213 413 6739
e-mail: pwebster-at-hei.org
http://www.hei.org/htm/aemi.htm


GUIDO LŸšND wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear readers,
} could anyone give me some hints or tips how I can separate a formvarfilm
} (0.5% in chloroform) from the glassslide ? Breathing or freezing doesn't
} help at all. It works better with non-washed slides, but then I get an
} uneven and dirty surface of the film.
} I appreciate every kind of support.
} Best regards
}
} Guido Luond
} Institute of Oral Microbiology and
} General Immunology
} Univ. of Zurich
} Plattenstr. 11
} CH-8028 Zurich, Switzerland
} E-mail: lueoend-at-zzmk.unizh.ch
}
}
}
}
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} Subject: TEM Formvar Film Cast on Glass
} From: GUIDO L=?ISO-8859-1?B?/PY=?=ND {lueoend-at-zzmk.unizh.ch}
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Paul Webster, Ph.D
House Ear Institute
2100 West Third Street
Los Angeles, CA 90057
phone:213 273 8026
fax: 213 413 6739
e-mail: pwebster-at-hei.org
http://www.hei.org/htm/aemi.htm



From daemon Fri Feb 11 18:30:19 2000



From: Brian McIntyre :      mcintyre-at-optics.rochester.edu
Date: Fri, 11 Feb 2000 14:01:04 -0500
Subject: metal pipe- inside surface

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hi all-

just got a request to view surface structure of the inside of a 12" metal
pipe. its too big for my chamber and they'd prefer a non-destructive
technique. will replication of the surface with acetate/acetone and SEM
work. i've never tried this before so any pointers would be welcome...

thx!
brian
----------------------------------------------
Brian McIntyre
Electron Microscopy Lab- River Campus
Univ of Rochester
Rochester, NY 14627
716-275-3058/4875



From daemon Fri Feb 11 18:30:26 2000



From: Walck. Scott D. :      walck-at-ppg.com
Date: Fri, 11 Feb 2000 16:55:15 -0500
Subject: Fluorescent dyes -Clueless in Pittsburgh

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Laurie,
Frieda Carson's book about histologic technique is the "gold standard for
histotechs right now. It is available at Amazon.com and Barnes and Noble's
website, and can be special ordered at the major bookstores, as it is
currently in print. (Unfortunately I am at home and do not have the actual
title in front of me)
Wanda Shotsberger
----- Original Message -----
} From: Laurie Wallin {lwallin-at-ucsd.edu}
To: {microscopy-at-sparc5.Microscopy.Com}
Sent: Thursday, February 10, 2000 11:03 AM


I was asked a couple of questions about fluorescent microscopy and I didn't
have a clue. Are all the dyes used with UV or are there other wavelengths
that can fluoresce them? Are the dyes used dangerous/hazardous? Are they
transparent to visible? Are they expensive? Can you get them in quantity?

-Clueless in Pittsburgh


Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)



From daemon Fri Feb 11 19:43:36 2000



From: Jeff Allbright :      jeff.allbright-at-usa.net
Date: Fri, 11 Feb 2000 16:04:05 -0600
Subject: looking for ETEC Company

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I knew a very good, reputable company in the Southern California region that
serviced ETEC SEMs. The name was Scan Service in California, Earl Weltmer
714 area code.

- Jeff

-----Original Message-----
} From: LAB. DE MICROSCOPIA ELECTRONICA - FI - UNER
[mailto:microsc-at-fi.uner.edu.ar]
Sent: Wednesday, February 09, 2000 5:10 AM
To: microscopy-at-sparc5.microscopy.com


Hello .....
we have an old ETEC Omniscan SEM, and we are searching for ETEC company or
any
people that can supply manuals ....

any help is welcome

best regards


===================================================
Fernando D. Balducci
Laboratorio de Microscopia Electr—nica
Facultad de Ingenier’a - Bioingenieria
Universidad Nacional de Entre Rios
Argentina
e-mail: microsc-at-fi.uner.edu.ar
tel: 54 43 975100
fax: 54 43 975077


===================================================




From daemon Fri Feb 11 18:30:33 2000



From: Chuck Butterick :      cbutte-at-ameripol.com
Date: 1/4/80 8:23 AM
Subject: questions for MSA members

Contents Retrieved from Microscopy Listserver Archives
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In regards to the diamond knives, both companies make an excellent
product and both provide good service. Because of price, the last 6
knives I've bought have been from Microstar (two well-used but dull
Diatome knives have been traded during these transactions) . All six
knives have been more than suitable for the intended purpose. I have
no commercial interest in either company.

Chuck Butterick
Engineered Carbons, Inc.


______________________________ Reply Separator _________________________________


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A colleague would like your responses to these questions. I believe that I
say something on the first question about a year ago and apologize for
asking again.


} Here's what I/we need to know.
}
} 1) Are Microstar diamond knives equal to Diatome. We have an offer that
} will save us about $1200 on three knives ( one 3mm ultra with 45 degree
} angle, one 3mm ultra with 35 degree angle, one 10 mm histo knife).
}
}
} 2) Is the power controller feature on the Pelco Microwave Tissue processor
} (model #3451) worth it? (costs about $1300 additional compared to the
} Pelco 3450 without the power controller). I plan to do immunolocalization
} work, but am not sure this power controller feature is necessary or worth
} the cost.
}
} Casey
}
}
}
Thanks for your time
}
Marty Reed
Equipment Technician
Biology Department
Humboldt State University
Arcata CA 95521
707-826-3234
707-826-3201 FAX
mmr7001-at-axe.humboldt.edu





From daemon Fri Feb 11 18:30:36 2000



From: White, Woody N :      Woody.N.White-at-mcdermott.com
Date: 2/11/00 1:01 PM
Subject: metal pipe- inside surface

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Date sent: Fri, 11 Feb 2000 14:01:04 -0500
To: Microscopy-at-sparc5.Microscopy.Com
} From: Brian McIntyre {mcintyre-at-optics.rochester.edu}


Depends on the detail needed, but acetate replication should work for almost
all
situations. Of course, everything you see will be inside out... Often,
selecting inverse video helps one perceive thing properly. If the pipe is
dirty or corroded, you will likely pick up some deposit with the replica.
This
can be distracting, but can also be helpful if x-ray analysis is required...
Woody White
McDermott Technology
My place: http://www.geocities.com/capecanaveral/3722

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hi all-

just got a request to view surface structure of the inside of a 12" metal
pipe. its too big for my chamber and they'd prefer a non-destructive
technique. will replication of the surface with acetate/acetone and SEM
work. i've never tried this before so any pointers would be welcome...

thx!
brian
----------------------------------------------
Brian McIntyre
Electron Microscopy Lab- River Campus
Univ of Rochester
Rochester, NY 14627
716-275-3058/4875



From daemon Fri Feb 11 18:30:38 2000



From: Mandayam V. Parthasarathy :      mvp2-at-cornell.edu
Date: Fri, 11 Feb 2000 18:23:11 -0500
Subject: PGT IMIX system module

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If any one has a PGT IMIX X-ray Analysis/Imaging system 4-40 (SUN Sparc)
that is in storage, or not in use, I would be interested in acquiring its
Image Signal Processor ISP module (Part# MO328D-00).



*******************************************************************

M.V. Parthasarathy
Prof. of Plant Biology, Adjunct Prof. of Biomedical Sciences (Vet), &
Director, Cornell Integrated Microscopy Center (CIMC)
Section of Plant Biology
228 Plant Science Building
Cornell University, Ithaca, NY 14853
E-Mail: mvp2-at-cornell.edu
Plant Biology Office: 268 Emerson; Telephone: 607-255-1734
Plant Biology Fax: 607-255-5407
CIMC Office: C1 054 Vet. Med. Center; Telephone: 607-253-3803
CIMC Office Fax: 607-253-3803
CIMC web site: http://www.cimc.cornell.edu




From daemon Sat Feb 12 03:29:53 2000



From: A.P. Alves de Matos :      apmatos-at-ip.pt
Date: Fri, 11 Feb 2000 19:12:34 -0600
Subject: TEM LKB control Unit

Contents Retrieved from Microscopy Listserver Archives
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Dear Brian

Stop destroying your pipe. With our large chamber SEM we can analyse your
pipe up to a length of 35 inches and a diameter of 28 inches. Maximum weight
of the sample should not exceed 500 pounds.

Because of the positionable electron gun it is easy to observe the inside
surface.

Best regards
Martin Klein

Disclaimer: VisiTec is the manufacturer of large chamber SEMs. And we like
large samples.

----------------------------------------------------------------------------
---
VisiTec Microtechnik GmbH
Karl-Marx-Str. 14
D-23936 Grevesmuehlen
Germany
Fon: +49-3881-79049
Fax: +49-3881-79048

email: info-at-visitec-em.de
WWW: http://www.visitec-em.de

----- Original Message -----
} From: Brian McIntyre {mcintyre-at-optics.rochester.edu}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Friday, February 11, 2000 8:01 PM


I am in desperate need of a control unit for a LKB ultratome III, since
mine is beyond repair.
I would appreciate if anyone coud sell me an unused unit from old
apparatus, even if it does not work, I might
be able to repair mine with the pieces.
Please reply to the e-mail below

Thanks

Dr. A.P. Alves de Matos
Dental Medical School
Lisbon University
apmatos-at-ip.pt




From daemon Sat Feb 12 03:29:45 2000



From: Brian Robertson :      brian.robertson-at-materials.oxford.ac.uk
Date: Fri, 11 Feb 2000 19:20:10 -0600
Subject: ELECTRON MICROSCOPY MATERIALS SPECIALIST

Contents Retrieved from Microscopy Listserver Archives
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ELECTRON MICROSCOPY MATERIALS SPECIALIST
UNL Center for Materials Research and Analysis

Analyze/characterize materials using electron microscopy, materials
preparation and computer instrumentation. Supervise/train students,
faculty and visiting researchers utilizing these methods. Bachelor's
with major in physical science, engineering or related field plus three
years experience in the operation, repair or design of electron
microscopes or other scientific instrumentation. Master's preferred.
Must have excellent computer and interpersonal/communication skills.
Knowledge of x-ray diffraction, TEM/SEM principles and sample
preparation experience preferred. Excellent benefits. Submit cover
letter, resume and names, addresses and telephone numbers of three
professional references to Professor David J. Sellmyer, 112 Brace Lab,
Lincoln, NE 68588-0113. Review of resumes will begin March 1. Position
will remain open until a suitable candidate is found. UNL is committed
to AA/EEO and ADA/504. If you require accommodation, please call (402)
472-8762.






From daemon Sat Feb 12 03:29:39 2000



From: michael baxter :      mykkb-at-juno.com
Date: Fri, 11 Feb 2000 19:21:23 -0600
Subject: Re: TEM-Formvar on Glass Slides

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Try a different brand of slide. Corning is the only brand we use in our EM
Class. There must be different kinds of surfactants used on them. Some
other brands of slides just don't release the formvar.
We usually wipe them off thoroughly with a Kimwipe only. Dip the slide
into the formvar and let dry. But don't dry for too long. Try using a
diamond tipped (or carbide) pen to score a "rectangle" on the slide.
Slowly insert the slide at about a 30 degree angle into the water. Wait
until you see the formvar start to release before you continue inserting
into the water.
This method usually works in our lab and for students.

Mike Baxter
Lehman College
Bronx, NY




From daemon Sun Feb 13 00:40:32 2000



From: Ken Tiekotter :      tiekotte-at-up.edu
Date: Sat, 12 Feb 2000 01:13:04 -0800 (PST)
Subject: Re: wanted: microtome

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Dear Linda,
I have a DuPont Sorvall MT2B or a Porter Blum MT1 I would be interested in
selling if the price is right. If you are interested, let me know via my
email address: tiekotte-at-up.edu.
Cheers!
Ken
-----------
Ken Tiekotter
Dept. of Biology
The University of Portland
5000 N. Willamette Blvd.
Portland, OR 97303

On Mon, 27 Aug 1956, Linda Chicoine wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Does anyone in the Connecticut area have an old microtome they would
} like to sell? I would be interested in anything from an LKB to RMC to
} Reichert/ and Leica. Please contact me by email. Thanks. Linda
} Chicoine
}
}
}



From daemon Sun Feb 13 00:40:33 2000



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Sat, 12 Feb 2000 03:32:42 -0600
Subject: SEM pipe replication

Contents Retrieved from Microscopy Listserver Archives
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} To: MICROSCOPY BB {Microscopy-at-sparc5.microscopy.com}
} Subject: SEM pipe replication
} Date: Sat, 12 Feb 2000 10:44:32 -0500
} From: "Garber, Charles A." {cgarber-at-2spi.com}
} X-Mailer: E-Mail Connection v3.1a
} Content-Length: 3106

}
} -- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --
}
} Brian McIntyre wrote:
} =========================================
} just got a request to view surface structure of the inside of a 12" metal
} pipe. its too big for my chamber and they'd prefer a non-destructive
} technique. will replication of the surface with acetate/acetone and SEM
} work. i've never tried this before so any pointers would be welcome...
} ==========================================
} You did not indicate how far into the pipe you want to do your looking,
but
} cellulose acetate is certainly one approach. The biggest problem you are
} going to have is to balance the need to do this all "nondestructively" vs.
} the need to see what might be underneath material covering the top surface

} (after all, it is the metal substrate that you really want to see). In
} other words, if the acetone should be a solvent for the contaminating
} material, (or if it lifts off metal oxide or other aspects of a scale) you
} will not be doing this very non-destructively. One technique is to make
} repetitive replicas on the same area, each additional one taking off more
of
} the contaminating material, in order to get to the bare metal surface.
But
} this might not be considered, by a court of law, to be nondestructive.
And
} if you are under an order to look at it nondestructively, this could
result
} in a problem with the court.
}
} When we have been faced with this dilemma, in order to first document what
} was there originally, we like to use our SPI "Wet Replica" kit, it is a
} silicone based system, and is described on our website. Certain dental
} replication systems such as "Sil-Flow" might work as well, but in general,
} for this kind of work, we believe our own kit is better. The silicone
resin
} in general won't remove surface contamination or scale. The drawback of
this
} technique is that after about 800X in the SEM you start to see "structure"
} from the replication system itself. A "plus" however is that you can make
a
} "replica of the replica", doing your SEM viewing on a positive, which does
} look just like the original inside surface.
}
} Once you have documented the state of the inside surface that way, you
} should be able to get permission to try the cellulose acetate replication
} method. There is additional information on the SPI Supplies website
relative
} to cellulose acetate replication methods.
}
} Disclaimer: SPI Supplies offers both cellulose acetate replicating tapes
} and sheets as well as the silicone based SPI "Wet Replica Kit" for use in
} electron microscopy applications.
}
} Chuck
}
} ============================================
}
} Charles A. Garber, Ph. D. Ph: 1-610-436-5400
} President 1-800-2424-SPI
} SPI SUPPLIES FAX: 1-610-436-5755
} PO BOX 656 e-mail:cgarber-at-2spi.com
} West Chester, PA 19381-0656 USA
} Cust.Service: spi2spi-at-2spi.com
}
} Look for us!
} ########################
} WWW: http://www.spi.cc
} ########################
} ============================================
}
}
}
}




From daemon Sun Feb 13 00:40:40 2000



From: Dagmar Preusse :      Preusse-at-chemie.uni-bremen.de
Date: Sat, 12 Feb 2000 12:54:22 +0100
Subject: permanent staining of LR-White embedded root sections

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Hi,
I made an embedding of the endodermis of an iris root with LR-Wihte and
would like to make ultrathin sections and stain them. My problem is how
to stain the sections and how to receive permantly stained sections. Is
there anyone who has experiences with the staining of suberin in the 3rd
endodermis? Please inform me about the handling.
Thank you so much
Dagmar Preusse



From daemon Sun Feb 13 00:40:43 2000



From: Wright, Cheryl W :      CWright-at-Sikorsky.com
Date: Sat, 12 Feb 2000 08:25:19 -0500
Subject: Photography of Coated Replicas

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I run a 1830 Amray S.E.M. and work with failed aircraft components. From
time to time I am asked to replicate a surface using the acetate tape &
acetone method. The coating I use is a combination of Au and Pd. My
problem is that when I have to stay in one particular area of the replica,
the beam leaves a nice little square embedded into the surface of the
replica. I try to rapidly focus in full field instead of partial field, and
use between 6 - 10Kv. Does anyone have any advise for me on how to avoid
the embedded square?

Thanks, Cheryl



From daemon Sun Feb 13 00:40:51 2000



From: Steve Chapman :      PROTRAIN-at-CompuServe.COM
Date: Sat, 12 Feb 2000 09:40:28 -0500
Subject: Photography of Coated Replicas

Contents Retrieved from Microscopy Listserver Archives
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Hi

It is the total current that you put into the specimen that is causing the
material to erode.

If you use a smaller spot size, smaller final aperture or smaller emission
current you should be better off? I do not know how good your SEM is at
low kV but if it was me I would use 2kV on this type of specimen; it will
be very sensitive even though it is coated.

To run happily at 2kV you need a filament to cathode distance that will
give you at least 30uA with your normal bias settings. If you do a good
deal of work on this type of specimen I would be inclined to lift the anode
by 5mm also. This will make the gun more efficient and make the job
easier.

Good luck

Steve Chapman
Protrain for Electron Microscopy Courses World Wide
E-mail - protrain-at-emcourses.com
Web Site - www.emcourses.com



From daemon Sun Feb 13 00:41:00 2000



From: Gordon Couger :      gcouger-at-rfdata.net
Date: Sat, 12 Feb 2000 13:32:41 -0600
Subject: [LM] replication

Contents Retrieved from Microscopy Listserver Archives
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I have been trying to develop a styrene replica
fluid so I can use polorized light.So far the
styrene clumps ups in little islands insted of
making a nice film. I am using acetone as
a solvent

Has anyone had any luck with this?

Gordon

Gordon Couger gcouger-at-couger.com

Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00




From daemon Sun Feb 13 16:26:42 2000



From: Mark Sanders :      msanders-at-biosci.cbs.umn.edu
Date: Sun, 13 Feb 2000 01:02:53 -0600
Subject: Re: questions for MSA members

Contents Retrieved from Microscopy Listserver Archives
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{fontfamily} {param} Monaco {/param} Marty,

I can address #2 with some confidence.  We have been using the
Pelco

Microwave Tissue processor (model #3451) for almost 2 years with the
power

controller and have found it essential for optimizing the procedures we
use

for antigen retrieval and immunolocalization at both the light and EM
level.

By being able to control the power you have much better control of the

"microwave effect" relative to heating. I would be happy to discuss
the

details with you directly.

Cheers,

Mark

****************************************************

Mark A. Sanders
           University
of Minnesota

Program Director
          Twin Cities
Campus

Imaging Center
            St.
Paul, MN  55108

23-35 Snyder Hall
         ph:
 612-624-3454

1475 Gortner Ave.
         fax:
612-624-1799

{/fontfamily} http://biosci.umn.edu/imagingcenter {fontfamily} {param} Monaco {/param}

President: Minnesota Microscopy Society

{/fontfamily} http://resolution.umn.edu/MMS/ {fontfamily} {param} Monaco {/param}



----------

} From: Marty Reed { {mmr7001-at-humboldt.edu}

} To: Microscopy-at-sparc5.Microscopy.Com

} Subject: questions for MSA members

} Date: Fri, Jan 4, 1980, 11:23 AM

}


}
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} The Microscopy ListServer -- Sponsor: The Microscopy Society of
America

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ListServer-at-MSA.Microscopy.Com

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}
-----------------------------------------------------------------------.

}

}

} A colleague would like your responses to these questions.  I
believe that I

} say something on the first question about a year ago and apologize
for

} asking again.

}

}

} }        Here's what I/we need to
know.

} }

} } 1)  Are Microstar diamond knives equal to Diatome.  We have
an offer that

} } will save us about $1200 on three knives ( one 3mm ultra with 45
degree

} } angle, one 3mm ultra with 35 degree angle, one 10 mm histo knife).

} }

} }

} } 2) Is the power controller feature on the Pelco Microwave Tissue
processor

} } (model #3451) worth it?  (costs about $1300 additional compared
to the

} } Pelco 3450 without the power controller).  I plan to do
immunolocalization

} } work, but am not sure this power controller feature is necessary or
worth

} } the cost.

} }

} } Casey

} }

} }

} }

} Thanks for your time

} }

} Marty Reed

} Equipment Technician

} Biology Department

} Humboldt State University

} Arcata CA 95521

} 707-826-3234

} 707-826-3201 FAX

} mmr7001-at-axe.humboldt.edu

}

}

{/fontfamily} Cheers, Mark
**************************************************** Mark A. Sanders
           University
of Minnesota Program Director
          Twin Cities
Campus Imaging Center
            St.
Paul, MN  55108 23-35 Snyder Hall
         ph:
 612-624-3454 1475 Gortner Ave.
         fax: 612-624-1799
http://biosci.umn.edu/imagingcenter President: Minnesota Microscopy
Society http://resolution.umn.edu/MMS/



{/x-rich}



From daemon Sun Feb 13 16:26:55 2000



From: Stuart McKernan :      mckernan-at-cems.umn.edu
Date: Sun, 13 Feb 2000 12:02:10 -0600 (CST)
Subject: Microscopy and Microanalysis 2000

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13 Feb 2000 09:14:55 PST
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------- Start of forwarded message -------

Rules, FAQ Docs - Microscopy ListServer
To: NewSub-at-sparc5.Microscopy.Com
} From: "Nestor J. Zaluzec" {zaluzec-at-sparc5.Microscopy.Com}

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To: NewSub-at-MSA.Microscopy.Com
} From: "Nestor J. Zaluzec" {zaluzec-at-microscopy.com}


Here is a final reminder and IMPORTANT CORRECTION for the Call for Papers for
Microscopy and Microanalysis 2000.

The title for symposium #05 in the Call for Papers was listed incorrectly. The
correct title should be "Phase Transitions" (If you check the symposium titles
on the electronic data submission forms it is listed correctly). X-ray
microanalysis papers should be directed to symposia 25 and 26.

The deadline for papers is Tuesday February 15, which is almost upon us.

Stuart McKernan (Program Chair)




__________________
Stuart McKernan stuartm-at-tc.umn.edu
Director Office:(612) 626-7594
IT Characterization Facility, University of Minnesota Desk:(612) 624-6009
100 Union Street S. E., Minneapolis, MN 55455 NEW-} Fax:(612) 625-5368



From daemon Sun Feb 13 20:38:21 2000



From: Mel Dickson :      m.dickson-at-unsw.edu.au
Date: Mon, 14 Feb 2000 12:29:53 +1100
Subject: metal pipe- inside surface

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} X-Sender: mcintyre-at-optics.rochester.edu
} Date: Fri, 11 Feb 2000 14:01:04 -0500
} To: Microscopy-at-sparc5.Microscopy.Com
} From: Brian McIntyre {mcintyre-at-optics.rochester.edu}
} Subject: metal pipe- inside surface
} Errors-to: Microscopy-request-at-sparc5.Microscopy.Com
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


The best way to make replicas is to use dental casting compound.

It has the ability to replicate very fine detail and accepts even damp
surfaces.

We used "Kerr Extrude Wash" (two part polyvinylsiloxane impression
material) to replicate (damp) tuna fish skin.

Once cured, we made epoxy positive casts from the negative casts, then
mounted, gold coated etc. for regular SEM.

Your local dental supply house will have this.

Kerr USA is at 28200 Wick Road, Romulus MI 48174-2600


Dr. Mel Dickson,
Deputy Director, The Electron Microscope Unit,
Adjunct Associate Professor, School of Microbiology & Immunology
The University of New South Wales Sydney, Australia.
Phone +612 9385 6383 Fax +612 9385 6400



From daemon Sun Feb 13 23:08:57 2000



From: Robert H. Olley :      r.h.olley-at-reading.ac.uk
Date: Mon, 14 Feb 2000 09:24:08 +0000 (GMT Standard Time)
Subject: Re: [LM] replication

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Dear Malcolm,

You could also consider Epson Expression 1600 Pro. There is a review at the
PC Magazine site:

http://www.zdnet.com/pcmag/stories/firstlooks/0,6763,2430926,00.html

True optical resolution 1600x3200. Dynamic range 3.3
Although it is not from a scientific point of view the authors claim that
this is the best scanner for the price they've ever seen.

Good luck,

Rado

Disclaimer: I'm not related to Epson company in any way.

---------------------------------------------------------------------
Radostin Danev
Laboratory of Ultrastructure Research
National Institute for Physiological Sciences
Myodaiji-cho, Okazaki 444-8585, JAPAN
e-mail: rado-at-nips.ac.jp
---------------------------------------------------------------------
----- Original Message -----
} From: Malcolm Haswell {malcolm.haswell-at-sunderland.ac.uk}
To: Microscopy (MSA) {microscopy-at-sparc5.microscopy.com}
Sent: Thursday, February 10, 2000 8:26 PM



On Sat, 12 Feb 2000, Gordon Couger wrote:

} I have been trying to develop a styrene replica
} fluid so I can use polarized light.So far the
} styrene clumps up in little islands instead of
} making a nice film. I am using acetone as
} a solvent

Polystyrene is only partially soluble in acetone, so the low MW goes into
solution while the high MW forms clumps or whatever. BUTANONE (MEK)
should work fine, but you'll have to give it perhaps 4 times the drying
time you'd need with acetone. For samples that are sensitive to polar
solvents such as these, you can also use toluene, but that takes an even
longer drying time.

+------------------------------------------------------------------------+
| Robert H.Olley Phone: |
| J.J.Thomson Physical Laboratory {direct line +44 (0) 118 9318572 |
| University of Reading {University internal extension 7867 |
| Whiteknights Fax +44 (0) 118 9750203 |
| Reading RG6 6AF Email: R.H.Olley-at-reading.ac.uk |
| England URL: http://www.reading.ac.uk/~spsolley |
+------------------------------------------------------------------------+




From daemon Mon Feb 14 14:21:57 2000



From: Robert H. Olley :      r.h.olley-at-reading.ac.uk
Date: Mon, 14 Feb 2000 09:35:52 +0000 (GMT Standard Time)
Subject: Re: Photography of Coated Replicas

Contents Retrieved from Microscopy Listserver Archives
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On Sat, 12 Feb 2000, Wright, Cheryl W wrote:

} I run a 1830 Amray S.E.M. and work with failed aircraft components. From
} time to time I am asked to replicate a surface using the acetate tape &
} acetone method. The coating I use is a combination of Au and Pd. My
} problem is that when I have to stay in one particular area of the replica,
} the beam leaves a nice little square embedded into the surface of the
} replica. I try to rapidly focus in full field instead of partial field, and
} use between 6 - 10Kv. Does anyone have any advise for me on how to avoid
} the embedded square?

Polystyrene MIGHT be a better material to use than cellulose acetate,
which tends to chemically self-destruct under electron beams: in fact,
when there is acetate remaining on replicas for TEM I sometimes burn it
off SLOWLY with the electron beam (too fast and you leave carbonaceous
stuff). The aromatic nature of PS also allows it to take up more energy.

You could replicate with PS, as follows:

Make a solution of PS in butanone (MEK) in a glass Petri dish. Allow to
evaporate slowly (if you can fume it off from a closed dish in an oven at
about 50^C, that's better). This film can then either be cast onto the
component with butanone, or you can melt-form it above the glass
transition of PS, say about 150^C. Don't use PS windows directly from
envelopes, because the films contain a lot of built-in-strain.

I'm not sure of it's performance in SEM, because I've used it for TEM
replication of acetone-sensitive specimens.

+------------------------------------------------------------------------+
| Robert H.Olley Phone: |
| J.J.Thomson Physical Laboratory {direct line +44 (0) 118 9318572 |
| University of Reading {University internal extension 7867 |
| Whiteknights Fax +44 (0) 118 9750203 |
| Reading RG6 6AF Email: R.H.Olley-at-reading.ac.uk |
| England URL: http://www.reading.ac.uk/~spsolley |
+------------------------------------------------------------------------+




From daemon Mon Feb 14 14:22:01 2000



From: MARK GERMANI :      mmrinc-at-wwa.com
Date: Mon, 14 Feb 00 08:15:48 -0600
Subject: Pittcon Short Course

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} From: "Walck. Scott D." {walck-at-ppg.com}

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So your companyÕs SEM operator just retired. You have used light microscopes for years and they have asked you to run the SEM also. So now what do you do? The SEM manuals are long gone and do you really want to crack open that 800 page SEM/x-ray microanalysis textbook?

DonÕt worry, consider taking Electron Microscopy and Microanalysis a one-day short course to be held on Sunday, March 12, at PITTCON 2000 in New Orleans. The course is an introduction to SEM, TEM and x-ray microanalysis and is designed for analytical chemists and others who need to use these techniques for industrial problem-solving and product research and development.

For further information and online registration visit www.pittcon.org (Course #2029) or contact me offline.

Mark S. Germani, Ph.D.
MicroMaterials Research, Inc.
136 Shore Drive
Suite 200
Burr Ridge, IL 60521

(630) 325-8170
(630) 325-8178 (fax)

mmrinc-at-wwa.com



From daemon Mon Feb 14 14:22:21 2000



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Mon, 14 Feb 2000 14:46:36 +0000 (GMT Standard Time)
Subject: Re: TEM LKB control Unit

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Electron Optical Services Ltd. make a replacement control
box. Their address is given below.

Dave



52 Higher Road,
Urmston,
Manchester,
M41 9AP
England
UK



Dave
On Fri, 11 Feb 2000 19:12:34 -0600 "A.P. Alves de Matos"
{apmatos-at-ip.pt} wrote:

} ------------------------------------------------------------------------
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} -----------------------------------------------------------------------.
}
}
} I am in desperate need of a control unit for a LKB ultratome III, since
} mine is beyond repair.
} I would appreciate if anyone coud sell me an unused unit from old
} apparatus, even if it does not work, I might
} be able to repair mine with the pieces.
} Please reply to the e-mail below
}
} Thanks
}
} Dr. A.P. Alves de Matos
} Dental Medical School
} Lisbon University
} apmatos-at-ip.pt
}
}
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"



From daemon Mon Feb 14 14:22:29 2000



From: Orth99-at-aol.com
Date: Mon, 14 Feb 2000 10:40:43 EST
Subject: Looking at the inside of metal pipes...

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How often does this community need to look at the inside of metal pipes or
pipes in general?

And, what is the typical surface roughness you are dealing with, i.e. would
an instrument with 100 um to 200 um of Z range be able to handle it?

And, what is the typical x,y range, i.e. would an instrument with 2 mm image
field be acceptable?

Just wondering. Thanks for any input you are willing to share.


Carol Rabke, Ph.D.
Surface Metrology Marketing & Applications Consultant
716-425-0976



From daemon Mon Feb 14 14:22:30 2000



From: Yvonne N“mcov‡ :      ynemcova-at-natur.cuni.cz
Date: Mon, 14 Feb 2000 16:53:33 +0100
Subject: embedding protocols

Contents Retrieved from Microscopy Listserver Archives
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Dear Microscopy Listserver members,
can someone provide me:
- a detailed protocol for embedding Chlamydomonas into the Lowicryl
Resin K4M (fixation media, desiccation solutions, times and
concentrations
that are suitable for Chlamydomonas)
- a fixation protocol for cryosectioning of Chlamydomonas

Thank you very much,
Yvonne Nemcova

--
*****************************************
Mgr. Yvonne Nemcova
Department of Botany, Charles University, Prague
Benatska 2. 128 01 Praha 2, Czech Republic
tel: 00-420-2-21953127, fax: 00-420-2-21953125
e-mail address: ynemcova-at-natur.cuni.cz
*****************************************




From daemon Mon Feb 14 14:22:59 2000



From: Virginie Serin :      serin-at-cemes.fr
Date: Mon, 14 Feb 2000 17:53:50 +0100
Subject: Re: EELS database, a new tool to play with

Contents Retrieved from Microscopy Listserver Archives
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Dear Scott,


Thank you for the information on the MSA/MAS format that you sent me
some weeks ago.


The MSA/MAS spectra file format is now implemented in the EELS and XAS
database, therefore the spectra can be downloaded in this format.
Please let me know if you have any other problems. Any more suggestions
?

Thank you for your help.


Best regards


Virginie









{center} -----------------------------------------------


{fontfamily} {param} New_York {/param} {bigger} {bigger} Virginie Serin

{/bigger} {/bigger} {/fontfamily} {fontfamily} {param} Geneva {/param} CEMES-CNRS,
29 Rue J. Marvig, BP 4347,

31 055 Toulouse Cedex 4, France

TŽl: 33 (0)5 62 25 78 67, Fax : 33 (0)5 62 25 79 99,

e-mail: serin-at-cemes.fr

http://www.cemes.fr


{/fontfamily} EELS and X ray database : http://www.cemes.fr/~eelsdb/


-----------------------------------------------

{/center}


{/x-rich}



From daemon Mon Feb 14 14:23:27 2000



From: tea meulia :      meulia.1-at-osu.edu
Date: Mon, 14 Feb 2000 14:54:32 -0500 (EST)
Subject: Edwards Vacuum Evaporator

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We are having an Edwards Vacuum Evaporator model E12E2 with a control unit
EVM052 in excess. This instrument has not been used for several year and it
may need some maintanance work. If you are interested please contact Tea
Meulia (meulia.1-at-osu.edu) or Pat Ashbaugh (ashbaugh.11-at-osu.edu).


Tea Meulia



Tea Meulia
Research Scientist and Head
Molecular and Cellular Imaging Center
OSU/OARDC
1680 Madison Ave.
Wooster OH 44691

tel.: (330) 263'3828
fax: (330) 202'3563

http://www.oardc.ohio-state.edu/mcic




From daemon Mon Feb 14 17:03:40 2000



From: Gordon Couger :      gcouger-at-rfdata.net
Date: Mon, 14 Feb 2000 15:24:38 -0600
Subject: Re: [LM] replication

Contents Retrieved from Microscopy Listserver Archives
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Thanks to all that responded. I have a lot better handle on how to
do it. I will report back my results.

Thanks to all for providing a great source of infomation.

Gordon

Gordon Couger gcouger-at-couger.com

Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00
----- Original Message -----
} From: "Robert H. Olley" {r.h.olley-at-reading.ac.uk}
} On Sat, 12 Feb 2000, Gordon Couger wrote:
}
} } I have been trying to develop a styrene replica
} } fluid so I can use polarized light.So far the
} } styrene clumps up in little islands instead of
} } making a nice film. I am using acetone as
} } a solvent
}
} Polystyrene is only partially soluble in acetone, so the low MW goes into
} solution while the high MW forms clumps or whatever. BUTANONE (MEK)
} should work fine, but you'll have to give it perhaps 4 times the drying
} time you'd need with acetone. For samples that are sensitive to polar
} solvents such as these, you can also use toluene, but that takes an even
} longer drying time.
}





From daemon Mon Feb 14 20:36:02 2000



From: Tom Reese :      treese-at-mbl.edu
Date: Mon, 14 Feb 2000 17:56:31 -0600
Subject: Stylus Pro 5000

Contents Retrieved from Microscopy Listserver Archives
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Epson has a new ink jet printer out-Stylus pro 5000-that is expensive
but they claim it is much better than the much cheaper models many of
us know and love. Would anyone know whether this is true of just
hype-hard to tell from the published specs...? Thanks...Tom Reese



From daemon Tue Feb 15 16:18:33 2000



From: Ken Tiekotter :      tiekotte-at-up.edu
Date: Tue, 15 Feb 2000 00:05:37 -0800 (PST)
Subject: Re: [LM] replication

Contents Retrieved from Microscopy Listserver Archives
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Dear Gordon,
Try xylene. It works quite nicely as a mounting medium of a differenting
refractive index from that of say, canada balsam or Permount. Email me if
you have questions. Good luck!
Cheers,
Ken

-------------
Ken Tiekotter
Dept. of Biology
The Univerisity of Portland
5000 N. Willamette Blvd.
Portland, OR 97303


On Sat, 12 Feb 2000, Gordon Couger wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I have been trying to develop a styrene replica
} fluid so I can use polorized light.So far the
} styrene clumps ups in little islands insted of
} making a nice film. I am using acetone as
} a solvent
}
} Has anyone had any luck with this?
}
} Gordon
}
} Gordon Couger gcouger-at-couger.com
}
} Stillwater, OK www.couger.com/gcouger
} 405 624-2855 GMT -6:00
}
}
}
}



From daemon Tue Feb 15 16:18:36 2000



From: Kuusisto, Ari :      Ari.Kuusisto-at-wallac.fi
Date: Tue, 15 Feb 2000 10:34:16 +0200
Subject: RE: autofluorescence quenching

Contents Retrieved from Microscopy Listserver Archives
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Corazon ,

You wrote
"I am working with archival specimens of formalin-fixed paraffin embedded
tissues and I am getting tremendous autofluorescence. I looked at tissue
sections after the sections have been de-waxed using either FITC or
rhodamine filter sets and red cells and connective tissues are brightly
fluorescent. Is there a way of suppressing the autofluorescence and still
retain reactivity of tissue to antibodies? I would appreciate any comments
or suggestions."

It is an unfortunate fact that most fixatives cause high autofluorescence
making the studies with conventional fluorescence microscope hard. The
disturbance of autofluorescence can be avoided by using time-resolved
fluorescence microscope and special labels (See the following articles:
VŠisŠlŠ M, SevŽus L, Kuusisto A, Harju R, and Soini
E: Time- resolved fluorescence microscopy: Elimination of autofluorescence
in tissue specimens for Image cytometry with fluorescent labelled probes.
Micron and Microscopica Acta 21; 3, (1990).

SevŽus L, Kuusisto A, VŠisŠlŠ M, HemmilŠ I., and
Soini E: Lanthanide
chelates as labels in in situ hybridization and
immunohistochemistry,
Micron and Microscopica Acta 21; 3, (1990).

SevŽus L, VŠisŠlŠ M, SyrjŠnen S, Sandberg M,
Kuusisto A, Harju R, Salo J, HemmilŠ I, Kojola H, and Soini E: Time-Resolved
Fluorescence Imaging of Europium Chelate Label in Immunohistochemistry and
In Situ Hybridization, Cytometry 13; 329, (1992).

SevŽus L, Kuusisto A, VŠisŠlŠ M, HemmilŠ I., Kojola
H., Roomans G. M. and Soini E.: Use of Fluorescent Europium Chelates as
Labels in Microscopy Allows Glutaraldefyde Fixation and Permanent Mounting
and Leads to Reduced Autofluorescence and Good Long-Time Stability,
Microscopy Research and Technique 27; 00, (1994).

I hope this information helps you.




Ari Kuusisto, Research Physicist

Phone: +358 2 267 8508 (direct)
+358 2 267 8111 (switchboard)
Fax: +358 2 267 8380
e-mail: Ari.Kuusisto-at-wallac.fi {mailto:Ari.Kuusisto-at-wallac.fi}
Address: Wallac Oy
PO Box 10
FIN-20101 Turku, Finland
Homepage: www.wallac.fi {http://www.wallac.fi}




From daemon Tue Feb 15 16:19:06 2000



From: Malcolm Haswell :      malcolm.haswell-at-sunderland.ac.uk
Date: Tue, 15 Feb 2000 14:02:33 +0000
Subject: Re: Stylus Pro 5000

Contents Retrieved from Microscopy Listserver Archives
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I saw the Epson web spec and it looks quite impressive - especially as
they seem to be quoting speeds for real picture printing, not 5%
coverage or text.

My only concern is that Epson now market two printers with "10 years
light fastness" (the Epson Stylus photo 870 - A4 and 1270 A3) so you
have to wonder which is better for e.m. prints.

Malcolm Haswell
Electron Microscopy
School of Sciences
Fleming Building
University of Sunderland
SUNDERLAND SR1 3SD
Tyne and Wear
UK

Tel (0191) 515 2872
e-mail: malcolm.haswell-at-sunderland.ac.uk

---------------------------------------------------------

Tom Reese wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Epson has a new ink jet printer out-Stylus pro 5000-that is expensive
} but they claim it is much better than the much cheaper models many of
} us know and love. Would anyone know whether this is true of just
} hype-hard to tell from the published specs...? Thanks...Tom Reese



From daemon Tue Feb 15 16:19:04 2000



From: marienhoff.visitec-at-t-online.de (Marienhoff)
Date: Tue, 15 Feb 2000 15:10:14 +0100
Subject: Re: Looking at the inside of metal pipes...

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The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Dear Carol,

We were looking up to 70 mm deep inside cylinder bores and engine blocks
several times during the last months with our large-chamber SEM. Roughness
was typically very small (less than 10 µm), but for small diameter tubes
the positioning system needs large travels, tilts, and rotary axes to inspect
every interesting area.

Disclaimer: Visitec manufactures the large-chamber SEM.

Best regards
Peter
___________________________
Dr. Peter Marienhoff
VisiTec Microtechnik GmbH
Karl-Marx-Str. 14
D-23936 Grevesmuehlen
Germany

Fon: +49-3881-790-47
Fax: +49-3881-790-48
email: pmarienhoff-at-visitec-em.de
http://www.visitec-em.de

"Orth99-at-aol.com"-at-sparc5.Microscopy.Com wrote:
} How often does this community need to look at the inside of metal pipes or
} pipes in general?
}
} And, what is the typical surface roughness you are dealing with, i.e. would
} an instrument with 100 um to 200 um of Z range be able to handle it?
}
} And, what is the typical x,y range, i.e. would an instrument with 2 mm image
} field be acceptable?
}
} Just wondering. Thanks for any input you are willing to share.
}
}
} Carol Rabke, Ph.D.
} Surface Metrology Marketing & Applications Consultant
} 716-425-0976
}



From daemon Tue Feb 15 16:19:08 2000



From: George Laing :      scisales-at-ngscorp.com
Date: Tue, 15 Feb 2000 10:12:15 -0500
Subject: Stylus Pro 5000

Contents Retrieved from Microscopy Listserver Archives
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The Epson 5000 is a printer/proofer using 6 color inkjet technology. The 6
color technology expands the available color gamut significantly. It is
available both with or without a Fiery RIP. The Fiery RIP is used for
Postscript processing of complex files from Quark, Pagemaker,
Illustrator,etc.
Without the Fiery box, an Epson software RIP is included. Print quality is
excellent although without the Fiery RIP, printing times can be
long,especially for 11x17 or larger prints. For more information see:
http://prographics.epson.com/products/stypro5000/index.html

Canon also has an excellent printer, the BJC-8500 that also uses 6 color
technology. The BJC-8500 also handles up to 13x19 paper with excellent
print quality. Canon utilizes a technology that optimizes ink drying and
water resistance. For more information see:
http://www.bjc8500.com/index2.html

Neither of these printers are speed demons in terms of page throughput, but
quality is excellent. I will be happy to answer any questions anyone may
have regarding output devices.

George Laing
National Graphic Supply

-----Original Message-----
} From: Tom Reese [mailto:treese-at-mbl.edu]
Sent: Monday, February 14, 2000 6:57 PM
To: Microscopy-at-sparc5.microscopy.com


Epson has a new ink jet printer out-Stylus pro 5000-that is expensive
but they claim it is much better than the much cheaper models many of
us know and love. Would anyone know whether this is true of just
hype-hard to tell from the published specs...? Thanks...Tom Reese




From daemon Tue Feb 15 16:19:10 2000



From: Dusevich, Vladimir :      DusevichV-at-umkc.edu
Date: Tue, 15 Feb 2000 09:25:24 -0600
Subject: RE: Photography of Coated Replicas

Contents Retrieved from Microscopy Listserver Archives
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I'd try, besides using low kV and beam current,
to increase substantially thickness of Au/Pd coating.

Vladimir


Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
3127 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy

} -----Original Message-----
} From: Wright, Cheryl W [mailto:CWright-at-Sikorsky.com]
} Sent: Saturday, February 12, 2000 7:25 AM
} To: 'Microscopy-at-MSA.Microscopy.Com'
} Subject: Photography of Coated Replicas
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListser} ver/FAQ.html
}
}
} --------------------------------------------------------------
} ---------.
}
}
} I run a 1830 Amray S.E.M. and work with failed aircraft
} components. From
} time to time I am asked to replicate a surface using the
} acetate tape &
} acetone method. The coating I use is a combination of Au and Pd. My
} problem is that when I have to stay in one particular area of
} the replica,
} the beam leaves a nice little square embedded into the surface of the
} replica. I try to rapidly focus in full field instead of
} partial field, and
} use between 6 - 10Kv. Does anyone have any advise for me on
} how to avoid
} the embedded square?
}
} Thanks, Cheryl
}



From daemon Tue Feb 15 16:19:28 2000



From: Pearl Martin :      image-at-optonline.net
Date: Tue, 15 Feb 2000 11:37:14 -0500
Subject: Job: Sr. Metallographic Tech SEM/X-ray

Contents Retrieved from Microscopy Listserver Archives
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I am a technical recruiter. Currently, I am working on a search for one
of my Northeast clients for a
Senior Metallographic Technician:


Will perform metallographic evaluation of coatings including work with
SEM (Scanning Electron
Microscopy) and X-ray. Will be responsible for the metallurgy
laboratory, including organization of
work, and operation and calibration of equipment. Will write technical
and engineering reports. Will
prepare projects for the metallurgy laboratory. Will maintain
communication with customers.

AAS/BS plus fours years experience in a metallography laboratory.

Resumes should be sent by email, mail or fax. For best results, please
send emails as attached
Word.docs - I have Word 97.

Pearl Martin
Image Associates Inc.
5254 Merrick Road
Massapequa, NY 11758
Phone (516)798-3993
Fax (516)797-8703
Email: pearl-at-jobspot.com



From daemon Tue Feb 15 16:19:32 2000



From: Furdanowicz, Waldemar :      rwafu-at-bsco.com
Date: Tue, 15 Feb 2000 12:19:14 -0500
Subject: RE: questions for MSA members (i.e. diamond knives)

Contents Retrieved from Microscopy Listserver Archives
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With regard to diamond knives, I think it depends on what you're cutting and
what quality of section you require.

We cut steel and cross sections of coatings (some very hard and brittle) on
steel, and the only knife that approaches satisfactory result is Diatome 35
degree, sharpened to extra fine edge. We traded our big old Microstar knife
for a 2nd Diatome, because it couldn't make good, electron transparent
sections.

On the other hand, if your material cuts as well with a glass knife as with
a diamond but you don't want to bother making your own glass knives, then go
ahead and save some dollars.

(In my view, the same reasoning applies to the brand of ultramicrotome to
use. My company is very frugal, and there is always strong pressure to save
a dollar here and there, but we found that only Reichert/Leica can
consistently produce good samples in our demanding application; and the
higher initial cost has been more than offset by savings in time and effort,
and by the quality of results. (I.e., often the ability to just get any kind
of a result).)

Valdemar Furdanowicz, Ph.D.
Research Labs - G165
Bethlehem Steel
Bethlehem PA 18016

No interest in Microstar, Diatome, Leica; and this not reflect an opinion or
position of my employer....etc.

-----Original Message-----
From: Chuck Butterick [mailto:cbutte-at-ameripol.com]
Sent: Friday, February 11, 2000 5:11 PM
To: Microscopy-at-sparc5.Microscopy.Com;
mmr7001-at-humboldt.edu
Subject: Re: questions for MSA members


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of America
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-----------------------------------------------------------------------.


In regards to the diamond knives, both companies make
an excellent
product and both provide good service. Because of
price, the last 6
knives I've bought have been from Microstar (two
well-used but dull
Diatome knives have been traded during these
transactions) . All six
knives have been more than suitable for the intended
purpose. I have
no commercial interest in either company.

Chuck Butterick
Engineered Carbons, Inc.


______________________________ Reply Separator
_________________________________
Subject: questions for MSA members
Author: Marty Reed {mmr7001-at-humboldt.edu} at INTERNET-MAIL
Date: 1/4/80 8:23 AM



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-----------------------------------------------------------------------.


A colleague would like your responses to these questions. I
believe that I
say something on the first question about a year ago and
apologize for
asking again.


} Here's what I/we need to know.
}
} 1) Are Microstar diamond knives equal to Diatome. We have
an offer that
} will save us about $1200 on three knives ( one 3mm ultra
with 45 degree
} angle, one 3mm ultra with 35 degree angle, one 10 mm histo
knife).
}
}
} 2) Is the power controller feature on the Pelco Microwave
Tissue processor
} (model #3451) worth it? (costs about $1300 additional
compared to the
} Pelco 3450 without the power controller). I plan to do
immunolocalization
} work, but am not sure this power controller feature is
necessary or worth
} the cost.
}
} Casey
}
}
}
Thanks for your time
}
Marty Reed
Equipment Technician
Biology Department
Humboldt State University
Arcata CA 95521
707-826-3234
707-826-3201 FAX
mmr7001-at-axe.humboldt.edu






From daemon Tue Feb 15 16:19:37 2000



From: Steve Barlow :      sbarlow-at-sunstroke.sdsu.edu
Date: Tue, 15 Feb 2000 09:47:15 -0800
Subject: deconvolution vendors

Contents Retrieved from Microscopy Listserver Archives
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We are considering the purchase of a separate deconvolution system to
augment our imaging lab's confocal system Any users or vendors with
information about such systems, please contact me offline.

thanks in advance

steve


Dr. Steven Barlow, Associate Director
EM Facility/Biology Department
San Diego State University
5500 Campanile Drive
San Diego CA 92182-4614
phone: (619)594-4523
fax: (619) 594-5676
email: sbarlow-at-sunstroke.sdsu.edu
website: http://www.sci.sdsu.edu/emfacility/

Chairman, Educational Outreach subcommittee
promoting access to microscopes
Microscopy Society of America http://www.msa.microscopy.com/




From daemon Tue Feb 15 16:19:39 2000



From: ERIC :      biology-at-ucla.edu
Date: Tue, 15 Feb 2000 10:37:36 -0800
Subject: Oven for curing blocks

Contents Retrieved from Microscopy Listserver Archives
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To the wealth of knowledge on the list,

We are in the market for a new oven for curing blocks.... Any
suggestions? This lab uses as a standard Eponate 12 as the epoxy resin of
choice....
The oven we currently have after being fixed several times does not see to
keep a constant temp.....




From daemon Tue Feb 15 16:19:48 2000



From: Wil Bigelow :      bigelow-at-engin.umich.edu
Date: Tue, 15 Feb 2000 15:11:33 -0400
Subject: Thermocouple gauges

Contents Retrieved from Microscopy Listserver Archives
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One of the members of this listserver group just asked me privately about
the fact that he had noticed that the reading of the thermocouple gauge on
his vacuum evaporator changed markedly when he jiggled the wires running
from the gauge tube to the gauge control unit. This is a matter that is
discussed in some detail on page 87 of my book 'Vacuum Methods in Electron
Microscopy' (see http://www.2spi.com/catalog/books/book48.html for a
description)

Actually, this is a rather common problem with thermocouple gauges which
all people using them should be aware of. It arises because the sensing
element in a thermocouple gauge is an arrangement of very fine wires that
form one or more thermocouple junctions. The output of these gauges is
thus the electrical potential produced by these junctions that is of the
order of only 10 or 15 millivolts DC. As a consequence, the signal detected
by the gauge controller can be strongly influenced by minor changes the
resistance of the electrical lines connecting the gauge to the controller.
Typically, unplugging one of the connectors in this line and reconnecting
it can change the resistance of the line sufficiently to cause a
significant change in the reading produced by the controller.

For this reason, considerable care must be exercised in installing and
maintaining thermocouple gauges if acceptable readings are to be obtained.
As a minimum, the same cables used in calibrating a gauge must subsequently
be used when it is put into operation, and the pins and sockets of the
plugs involved in the circuit must be kept clean and firmly connected.

Because of the low DC voltages that must be detected, the meters used in
the gauge circuit must be rather delicate and sensitive. We have
encountered situations when a static electric charge on the plastic window
of a meter affected the movement of the needle inside the meter enough to
prevent an accurate response. Coating the meter window with an anti-static
solution cured this situation.

As discussed in my book, Thermocouple gauges are rugged, inexpensive, and
very convenient for use in many applications, but they must be handled with
care if they are to give acceptable service.

Wilbur C. Bigelow, Prof. Emeritus
Materials Sci. & Engr., University of Michigan
Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu;
Fx:734-763-4788; Ph:734-662-5237




From daemon Tue Feb 15 20:09:28 2000



From: Karen Kavanagh :      kavanagh-at-sfu.ca
Date: Tue, 15 Feb 2000 14:26:57 -0800 (PST)
Subject: TEM available Philips EM-300

Contents Retrieved from Microscopy Listserver Archives
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A working Philips EM 300 TEM is available for donation to
a non-profit organization.
Please contact Karen Kavanagh at
kavanagh-at-sfu.ca




From daemon Tue Feb 15 20:09:41 2000



From: Heather A Owen :      owenha-at-csd.uwm.edu
Date: Tue, 15 Feb 2000 18:02:57 -0600 (CST)
Subject: TEM - glutaraldehyde for perfusion

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Hello everyone,

A graduate student is getting ready for a rather large scale preparation
of rat brains for TEM using vascular perfusion. Luckily (since I'm a
botanist) her major professor has had experience with the procedure. He
doesn't know for sure whether he used EM or Biological grade
glutaraldehyde previously, but considering the large volume that will be
required, and the cost difference, I'm hoping that the Biological grade
will suffice.

I would appreciate advice from those of you that have experience with this
method of fixation.

Thank you in advance for any information.

Heather Owen

Heather A. Owen, Director
Electron Microscope Laboratory
Department of Biological Sciences
University of Wisconsin - Milwaukee
(414)229-6816




From daemon Tue Feb 15 20:09:42 2000



From: JosŽ Ull‡n Serrano :      jullan-at-mixcoac.upmx.mx
Date: Tue, 15 Feb 2000 18:05:34 -0600
Subject: epifluorescence

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Dear Colleagues:

I have attempted to purchase an epifluorescence nose from 'Carl Zeiss'
to fit the old "Universal" model scopes, but was dismayed to find out that
it is no longer available.
If anyone has one that is not being used and wishes to sell it, I would be
very interested.

Thanks

JOSE ULLAN SERRANO, Ph.D., M.D.
ÑÑÑÑÑÑÑÑÑÑÑÑÑÑÑÑ
E-mail: jullan-at-mixcoac.upmx.mx

Departamento de ANATOMIA
Escuela de MEDICINA
Universidad Panamericana
c/ Donatello, 59
Col. Insurgentes-Mixcoac
03920 MEXICO, D.F.
ÑÑÑÑÑÑÑÑÑÑÑÑÑÑÑ
http://www.mixcoac.upmx.mx
Telef. 55 98 33 02 / 55 63 13 43
FAX: 56 11 35 85



From daemon Wed Feb 16 00:57:58 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Tue, 15 Feb 2000 20:05:09 -0600
Subject: Re: Thermocouple gauges

Contents Retrieved from Microscopy Listserver Archives
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Thermocouple gauges are more like gross indicators of vacuum. As a
resistive bridge, they are driven by constant current sources and the
bridge or sensor output is fed to a differential amplifier. This amplifier
has a gain and zero adjust potentiometer. If either of these are dirty
or defective, readings will be off. But if a thermocouple sensor is
exhibiting erratic readings, and it uses the standard vacuum tube socket
connector, I'd replace the detector right off the bat. They do eventually
fail. A $25 throw away isn't worth the hassle.

Most instruments, like SEMs, use the TC gauge to indicate that the mechanical
pump is working. Once the turbo pump spins up to } 80% RPM, the TC gauge
is rather useless. Better readings are from cold cathode gauges....which tend
not to fire very regularly. Hence, the optimum readings are from the ion pump
current.

At 01:11 PM 2/15/00 , you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Wed Feb 16 00:58:02 2000



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Tue, 15 Feb 2000 20:13:52 -0800
Subject: TEM - glutaraldehyde for perfusion

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Heather

We perfuse 4% formaldehyde in 1x PBS only, than prepared the brain's slices
.3-.4 mm thick and fix them in a mixture 3-4% formaldehyde 2% GA (all - EM
grade) in 1x PBS. If you would like to add GA into perfusion solution - it
is only 0.1% - not a big deal. From another hand, I don't think Biological
grade GA will affects fixation quality. But, who knows, every time it is a
"game": if fixation is good - no problem, if - no, we will start to think
what's going on and "biological" grade may be a "case"... To avoid such
troubles I always used fresh GA and formaldehyde from non-opened ampoules.

Good luck, Sergey


} Date: Tue, 15 Feb 2000 18:02:57 -0600 (CST)
} From: Heather A Owen {owenha-at-csd.uwm.edu}
} Subject: TEM - glutaraldehyde for perfusion
} To: MSA Listserver {Microscopy-at-sparc5.Microscopy.Com}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu
http://www.bol.ucla.edu/~sryazant




From daemon Wed Feb 16 00:58:04 2000



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Tue, 15 Feb 2000 21:20:05 -0800
Subject: Thermocouple gauges

Contents Retrieved from Microscopy Listserver Archives
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Dear Wilbur

As for my knowledge (mostly from physic's course at 7th grade Russian
school) the thermocouple gauge has two "thermocouples": the probe itself
and reference thermocouple inside of gauge (separate "references" for each
type of probe, I believe). All together they generate enough response to
be easily measured. I agree that the major problem in your case is a bad
electrical contact in the connectors or even broken wire (it is well
possible, because some material uses for thermocouples are very fragile).
In my point of view, thermocouple gauges is very reliably and most accurate
devices and they do not need any special care.

I am using BARNANT-100 Thermocouple Gauge and REX-P96 Thermocouple
Controller with type "T" thermocouple sensors (copper/constantan, I
believe, but not sure) for many years without any problem. The sensor goes
into the vacuum chamber through regular 8-pin feed-thought. I was using
same as thermocouple material for screws to connect thermocouple's wires to
the feed-though wiring. For some reason, soldering does not work. I took
that screws from disassembled thermocouple connectors (looks like small
plugs) most suppliers sells for thermocouple probes (a dollar each). That
connectors should correspond to the thermocouple probe you are using.
Usually those connectors are color-coded. For instance connectors for my
type "T" probe was coded in blue. BARNANT-100 Thermocouple Gauge and
REX-P96 Controller performs self-calibration test every time you shut it ON
or disconnect/connect probe. During that calibration they "compensate"
some electrical abnormalities in the circuit like difference in the probe
length or as in my case the current deviation on the enter and end of the
feed-through. In theory, the "break" in thermocouple wiring on
feed-through should affect the gauge accuracy. In practice, I did not find
a difference between "solid" and with-feed-though probe up to 0.1oC
accuracy (which is sufficient for my needs). REX-P96 Thermocouple
Controller is just amazing. You may program complicated profiles for
cooling/heating cycles and it holds temperature pretty well. It has
special auto-calibration function to adjust heat rate depending from
parameters of your system. In practice it mean that your sample will not
overheated because of heater inertia. I am using that Controller in my
high-resolution shadowing device to control temperature from -150 to +50oC.

I have no interest in described products (only happy user) mostly because I
even don't know the names of real manufacturers. I remember it was
distributed by Cole-Parmer.

Sergey



} Date: Tue, 15 Feb 2000 15:11:33 -0400
} From: Wil Bigelow {bigelow-at-engin.umich.edu}
} Subject: Thermocouple gauges
} X-Sender: bigelow-at-srvr5.engin.umich.edu
} To: Microscopy Listserver {microscopy-at-sparc5.Microscopy.Com}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu
http://www.bol.ucla.edu/~sryazant




From daemon Wed Feb 16 16:21:13 2000



From: Gordon Couger :      gcouger-at-rfdata.net
Date: Wed, 16 Feb 2000 01:53:56 -0600
Subject: Re: [LM] replication Thanks!!

Contents Retrieved from Microscopy Listserver Archives
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MEK worked great. I broke up the clear plastic from a
CDROM case and made a thick solution by adding MEK.
cleaned a slide and spread a drop of the solution on the
slide and put a celery leaf on it I covered the whole thing
with saran warp and gently pressed another slide on it
to get good contact. Then removed the slide and Saran
wrap.

After drying about 30 minutes I pealed the leaf off and
took a look under a Nomarski phase scope. It was
the best image I have seen on the scope. The Nomarski
colors were extremely strong and I could get three bands
of color in some features/artifacts. The effect of the styrene
on the polarized light were not as strong as I had hoped for
but they do increase the contrast and Nomarski colors. It
will make lovely photos. Now if my color CCD camera would
just get here. Monochrome just won't do it justice.

I has been a long time since I looked at a leaf but the
stoma showed up just like the books show and I remember.
Individual cells were very clearly visible.

The film is extremely fragile and probably needs a little
plastizer added and probably go to a go to a multi coat
system if the film needs to be pealed from a surface and
mounted. with the first coats being thin with little or no
plastizier and the following coats being thicker and
carrying more plastisizer.

My thanks to all the helped on this. I will probably try
other solvents but MEK does not set off my asthma
like xylene and toluene do. So I will probably put up
with the slower drying times.

I was very impressed with the detail that was replicated.

Thanks
Gordon

Gordon Couger gcouger-at-couger.com

Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00




} From: Robert H. Olley [mailto:r.h.olley-at-reading.ac.uk]
} On Sat, 12 Feb 2000, Gordon Couger wrote:
}
} } I have been trying to develop a styrene replica
} } fluid so I can use polarized light.So far the
} } styrene clumps up in little islands instead of
} } making a nice film. I am using acetone as
} } a solvent
}
} Polystyrene is only partially soluble in acetone, so the low MW goes into
} solution while the high MW forms clumps or whatever. BUTANONE (MEK)
} should work fine, but you'll have to give it perhaps 4 times the drying
} time you'd need with acetone. For samples that are sensitive to polar
} solvents such as these, you can also use toluene, but that takes an even
} longer drying time.





From daemon Wed Feb 16 16:21:19 2000



From: Ken Tiekotter :      tiekotte-at-up.edu
Date: Wed, 16 Feb 2000 01:20:42 -0800 (PST)
Subject: Re: epifluorescence

Contents Retrieved from Microscopy Listserver Archives
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Dear Josi,
Try contacting John Oren at Vermont Optechs. I cannot tell you
immediately what his email or web site address is but his mail address is:
P.O. Box 69, Charlotte, VT 05445: Tel. (802) 425-2040; FAX. (802)
425-2074. Good luck!
-Ken


On Tue, 15 Feb 2000, Jos[ISO-8859-1] é Ullán Serrano wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} Dear Colleagues:
}
} I have attempted to purchase an epifluorescence nose from 'Carl Zeiss'
} to fit the old "Universal" model scopes, but was dismayed to find out that
} it is no longer available.
} If anyone has one that is not being used and wishes to sell it, I would be
} very interested.
}
} Thanks
}
} JOSE ULLAN SERRANO, Ph.D., M.D.
} ‹‹‹‹‹‹‹‹‹‹‹‹‹‹‹‹
} E-mail: jullan-at-mixcoac.upmx.mx
}
} Departamento de ANATOMIA
} Escuela de MEDICINA
} Universidad Panamericana
} c/ Donatello, 59
} Col. Insurgentes-Mixcoac
} 03920 MEXICO, D.F.
} ‹‹‹‹‹‹‹‹‹‹‹‹‹‹‹
} http://www.mixcoac.upmx.mx
} Telef. 55 98 33 02 / 55 63 13 43
} FAX: 56 11 35 85
}
}



From daemon Wed Feb 16 16:21:18 2000



From: Alexander Mironov Jr. :      amironov-at-cmns.mnegri.it
Date: Wed, 16 Feb 2000 10:28:42 +0100 (MET)
Subject: TEM, biology: Diatome knives

Contents Retrieved from Microscopy Listserver Archives
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Dear All,

I have heard recently that Diatome invent some coating of their diamond
knives that significantly improve the quality of cryosections (they appear
more smooth, less compressed, practically without folds). Is it true or
just usual story to increase sales?

Sincerely,
Dr. Alexander A. Mironov Jr., MD, PhD
Unit of Morphology
Dept. of Cell Biology and Oncology
Consorzio Mario Negri Sud
Via Nazionale, S.Maria Imbaro (Ch)
66030 Italy

Tel. 0039-0872-570-332
Fax 0039-0872-570-412
E-mail: amironov-at-cmns.mnegri.it




From daemon Wed Feb 16 16:21:28 2000



From: David_Bell-at-Millipore.com
Date: Wed, 16 Feb 2000 08:25:55 -0500
Subject: Re: RJ Lee Instruments: Many Thanks!

Contents Retrieved from Microscopy Listserver Archives
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Hello again,

I want to thank everyone who took the time to reply to me. Your emails were
very informative and helpful, and I passed them along to my coworker.

Thanks again,

David Bell
Scientist
Electron Microscopy Lab
Millipore Corporation
80 Ashby Road
Bedford, MA 01730
(781) 533-2108




From daemon Wed Feb 16 16:21:33 2000



From: rlvaughn-at-unmc.edu
Date: Wed, 16 Feb 2000 08:48:39 -0600
Subject: TEM repair services

Contents Retrieved from Microscopy Listserver Archives
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Speaking of thermocouples, I need to replace (probably) the T1 and T2
thermocouples in our OLD Denton evaporator. Where, other than Denton, can
I look for these?
I also need service work done on our old Ultracut (the model before the "E"
series). I refuse to work with Leica. Is there anyone in the "real"
midwest area?



From daemon Wed Feb 16 16:21:40 2000



From: Bede Willenbring :      Bede.Willenbring-at-HBFuller.com
Date: Wed, 16 Feb 2000 09:20:53 -0600
Subject: Re: Thermocouple gauges

Contents Retrieved from Microscopy Listserver Archives
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I've also seen this type of behavior caused by a "cold" solder joint on one or more of the wires in the guage connector socket. Re-flowing the joints did wonders for stability.



============================================================
Bede Willenbring Phone: (651)236-5470
H.B. Fuller Company FAX: (651)236-5430
Research Chemist E-mail: Bede.Willenbring-at-HBFuller.com
P.O. Box 64683
St. Paul, MN 55164-0683



} } } Wil Bigelow {bigelow-at-engin.umich.edu} 02/15 1:11 PM } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


One of the members of this listserver group just asked me privately about
the fact that he had noticed that the reading of the thermocouple gauge on
his vacuum evaporator changed markedly when he jiggled the wires running
from the gauge tube to the gauge control unit. This is a matter that is
discussed in some detail on page 87 of my book 'Vacuum Methods in Electron
Microscopy' (see http://www.2spi.com/catalog/books/book48.html for a
description)

Actually, this is a rather common problem with thermocouple gauges which
all people using them should be aware of. It arises because the sensing
element in a thermocouple gauge is an arrangement of very fine wires that
form one or more thermocouple junctions. The output of these gauges is
thus the electrical potential produced by these junctions that is of the
order of only 10 or 15 millivolts DC. As a consequence, the signal detected
by the gauge controller can be strongly influenced by minor changes the
resistance of the electrical lines connecting the gauge to the controller.
Typically, unplugging one of the connectors in this line and reconnecting
it can change the resistance of the line sufficiently to cause a
significant change in the reading produced by the controller.

For this reason, considerable care must be exercised in installing and
maintaining thermocouple gauges if acceptable readings are to be obtained.
As a minimum, the same cables used in calibrating a gauge must subsequently
be used when it is put into operation, and the pins and sockets of the
plugs involved in the circuit must be kept clean and firmly connected.

Because of the low DC voltages that must be detected, the meters used in
the gauge circuit must be rather delicate and sensitive. We have
encountered situations when a static electric charge on the plastic window
of a meter affected the movement of the needle inside the meter enough to
prevent an accurate response. Coating the meter window with an anti-static
solution cured this situation.

As discussed in my book, Thermocouple gauges are rugged, inexpensive, and
very convenient for use in many applications, but they must be handled with
care if they are to give acceptable service.

Wilbur C. Bigelow, Prof. Emeritus
Materials Sci. & Engr., University of Michigan
Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu;
Fx:734-763-4788; Ph:734-662-5237






From daemon Wed Feb 16 16:21:44 2000



From: Geoff McAuliffe :      mcauliff-at-UMDNJ.EDU
Date: Wed, 16 Feb 2000 11:02:40 -0500
Subject: Re: TEM - glutaraldehyde for perfusion

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


John Heckman wrote:

} Heather A Owen wrote:
}
} } Hello everyone,
} }
} } A graduate student is getting ready for a rather large scale preparation
} } of rat brains for TEM using vascular perfusion. Luckily (since I'm a
} } botanist) her major professor has had experience with the procedure. He
} } doesn't know for sure whether he used EM or Biological grade
} } glutaraldehyde previously, but considering the large volume that will be
} } required, and the cost difference, I'm hoping that the Biological grade
} } will suffice.
} }
} } Heather,
}
} It's been a while but, back when I did such things, I had bouts of
} non-uniform fixation when using biological grade glutaraldehyde solutions
} even in plant tissues. However, the solutions had been kept much longer than
} I'd now use and there are other preparation technique changes that confound
} the determination of causality. In the cases where I've perfused small
} animals, I've used EM grade GA since, as expensive as it is, it's cheaper
} than repeating the experiment.
}
} However, I did a full systemic perfusion. One random thought ( I haven't
} tried it): What's the vascular volume of a rat brain? If you just need just
} the brain, I'd think a little creative clamping (really small hemostats?) in
} the thorax should let you reduce the volume needed to make even a large
} experiment affordable with "the good stuff".
}
} cheers and good luck,
} John
}

Heather:

I agree with John on both counts. We clamp the aorta (and anything eles that
gets in the way) high in the abdomen to avoid perfusing organs we are not
interested in. Saves time and money. Also we tilt the animal once perfusion is
underway so that the head is lower.

Geoff
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************




From daemon Wed Feb 16 16:21:45 2000



From: Roger Moretz :      rcmoretz-at-excite.com
Date: Wed, 16 Feb 2000 08:21:17 -0800 (PST)
Subject: Re: TEM - glutaraldehyde for perfusion

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have had a lot of years' experience doing vascular perfusion of the CNS of
rodents (rat and mouse). We have used the EM Sciences Biological Grade
glutaraldehyde, 50%, as our primary source for perfusion without problems
related to fixation. However, we have not used straight glutaraldehyde, but
found that 2% paraformaldehyde + 1% glutaraldehyde in 0.1M cacodylate buffer
at pH 7.0 to 7.2 gave consistent results with excellent preservation of
structures, morphology and cytoplasmic and nuclear components. We have used
cacodylate primarily because we use uranyl acetate stain either in the
dehydration/processing protocol and/or on the thin sections. Use of
phosphate buffers invariably results in precipitate formation, even when
used only on sections. Another reason for the paraform/glut/cacodylate
combination is that we have studied the leakage of tracers (e.g. HRP,
microperoxidase) across the blood brain barrier, and had to be able to move
into Tris buffer for DAB reactions, and phosphate buffer gave consistently
poorer results.

Hope this helps.

Roger Moretz
Dept. of Toxicology
BI Pharmaceuticals, Inc.

I have no personal or financial interest in EM Sciences--just a satisfied
user.


On Tue, 15 Feb 2000 18:02:57 -0600 (CST), Heather A Owen wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hello everyone,
}
} A graduate student is getting ready for a rather large scale preparation
} of rat brains for TEM using vascular perfusion. Luckily (since I'm a
} botanist) her major professor has had experience with the procedure. He
} doesn't know for sure whether he used EM or Biological grade
} glutaraldehyde previously, but considering the large volume that will be
} required, and the cost difference, I'm hoping that the Biological grade
} will suffice.
}
} I would appreciate advice from those of you that have experience with
this
} method of fixation.
}
} Thank you in advance for any information.
}
} Heather Owen
}
} Heather A. Owen, Director
} Electron Microscope Laboratory
} Department of Biological Sciences
} University of Wisconsin - Milwaukee
} (414)229-6816
}
}
}


Roger C Moretz, Ph.D.





_______________________________________________________
Get 100% FREE Internet Access powered by Excite
Visit http://freeworld.excite.com



From daemon Wed Feb 16 16:21:34 2000



From: John Heckman :      heckman-at-pilot.msu.edu
Date: Wed, 16 Feb 2000 10:01:07 -0800
Subject: Re: TEM - glutaraldehyde for perfusion

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Heather A Owen wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hello everyone,
}
} A graduate student is getting ready for a rather large scale preparation
} of rat brains for TEM using vascular perfusion. Luckily (since I'm a
} botanist) her major professor has had experience with the procedure. He
} doesn't know for sure whether he used EM or Biological grade
} glutaraldehyde previously, but considering the large volume that will be
} required, and the cost difference, I'm hoping that the Biological grade
} will suffice.
}
} I would appreciate advice from those of you that have experience with this
} method of fixation.
}
} Thank you in advance for any information.
}
} Heather Owen
}
} Heather A. Owen, Director
} Electron Microscope Laboratory
} Department of Biological Sciences
} University of Wisconsin - Milwaukee
} (414)229-6816

Heather,

It's been a while but, back when I did such things, I had bouts of
non-uniform fixation when using biological grade glutaraldehyde solutions
even in plant tissues. However, the solutions had been kept much longer than
I'd now use and there are other preparation technique changes that confound
the determination of causality. In the cases where I've perfused small
animals, I've used EM grade GA since, as expensive as it is, it's cheaper
than repeating the experiment.

However, I did a full systemic perfusion. One random thought ( I haven't
tried it): What's the vascular volume of a rat brain? If you just need just
the brain, I'd think a little creative clamping (really small hemostats?) in
the thorax should let you reduce the volume needed to make even a large
experiment affordable with "the good stuff".

cheers and good luck,
John

John Heckman
MSM Department
Michigan State University





From daemon Wed Feb 16 16:21:52 2000



From: Lesley Weston :      lesley-at-interchange.ubc.ca
Date: Wed, 16 Feb 2000 10:46:29 -0800 (PST)
Subject: Re: TEM - glutaraldehyde for perfusion

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We use Biological grade to perfuse rats (whole body, we haven't tried clamping
bits off, though that might be a good idea since we're interested in just the
head). After the perfusion is finished, we remove the interesting bit, which is
huge by EM standards, and fix some more in Karnowski's. Then we go on to osmium,
and continue processing. We get acceptable EM pictures at high magnification,
even though our blocks are so large and processing times are therefore
extended. So I can recommend Biological grade glutaraldehyde. Hope this helps.

Lesley Weston.



On Tue, 15 Feb 2000, Heather A Owen wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hello everyone,
}
} A graduate student is getting ready for a rather large scale preparation
} of rat brains for TEM using vascular perfusion. Luckily (since I'm a
} botanist) her major professor has had experience with the procedure. He
} doesn't know for sure whether he used EM or Biological grade
} glutaraldehyde previously, but considering the large volume that will be
} required, and the cost difference, I'm hoping that the Biological grade
} will suffice.
}
} I would appreciate advice from those of you that have experience with this
} method of fixation.
}
} Thank you in advance for any information.
}
} Heather Owen
}
} Heather A. Owen, Director
} Electron Microscope Laboratory
} Department of Biological Sciences
} University of Wisconsin - Milwaukee
} (414)229-6816
}
}
}
}




From daemon Wed Feb 16 20:58:18 2000



From: Larry Nittler :      nittler-at-lepvax.gsfc.nasa.gov
Date: Wed, 16 Feb 2000 17:09:21 -0500
Subject: TEM - Looking for ion mill

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello microscopy listserver-type people,

I am in the market for a used ion mill. Can anybody point me to one?

Thanks,
Larry

--
------------------------------------------------------------------------------
Larry R. Nittler Laboratory for Extraterrestrial Physics,
Code 691
Interstellar Dust Buster NASA Goddard Spaceflight Center
Greenbelt MD 20771
phone: 301-286-4572 fax:301-286-0212
nittler-at-lepvax.gsfc.nasa.gov http://www.ciw.edu/lrn/
------------------------------------------------------------------------------



From daemon Wed Feb 16 20:58:19 2000



From: RLevenson-at-cri-inc.com
Date: Wed, 16 Feb 2000 16:24:20 -0600
Subject: RE: autofluorescence quenching: spectral imaging can help

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} From: RLevenson-at-cri-inc.com Subject: RE: autofluorescence quenching:
spectral imaging can help

You asked about strategies for reducing autofluorescence.

In addition to various strategies which can include using near-IR dyes like
Cy5.5 and exciting far in the red, you can also treat tissues with sodium
borohydride or toluidine blue (these latter observations I pass on from
others--have not tried them myself).

I have recently had very promising results using spectral imaging to
discriminate autofluorescence from desired fluorescence. [Caution: I am
describing a product sold by my company]. CRI makes a liquid crystal
tunable filter (LCTF) that can be used to collect a stack of images at
different wavelengths. This yields a spectrum at every pixel of an image.
(A product with similar capabilities is sold by Applied Spectral Imaging,
but uses a different technology.) CRI's new LCTF, the FluoroSpec, uses
special optics to combine polarization states; along with other
improvements, it has more than twice the light throughput compared to
previous models.

The sample was formalin-fixed brain probed with a Cy2-labeled antibody to a
low-abundance protein. By eye it was very difficult to distinguish the Cy2
signal from the bright green to green-orange autofluorescence. Using a
spectral data set (15 images from 510 nm to 650 nm at 10 nm intervals) and
linear combination algorithms for pixel-unmixing, it was possible to
completely separate the Cy2 and autofluorescence images.

I would be glad to share these images with any interested parties.

Richard


Richard Levenson, MD
Technical Director, Biomedical Systems
Cambridge Research & Instrumentation (CRI), Inc.
80 Ashford St.
Boston, MA 02134

Ph: (617) 787-5700; Fax: (617) 787-4488
rlevenson-at-cri-inc.com www.cri-inc.com




From daemon Wed Feb 16 20:58:23 2000



From: chuck burilla :      burilact-at-ix.netcom.com
Date: Wed, 16 Feb 2000 18:29:41 -0500
Subject: Re: Stylus Pro 5000

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


With respect to the Epson Stylus Pro 5000:

I just purchased one for printing images, x-ray maps, etc. obtained from my
new JEOL 8900 microprobe. Previous to this I was utilizing an Epson Photo
700 printer with my older Cameca MBX probe. I was very pleased with the 700
and for a few hundred dollars sure was worth it! Not a fast printer but gave
high quality photo-type prints.
After reading the 5000 specs I decided to get one for the new probe.
In a "nutshell" here are my observations:

I purchased just the 5000 printer (not the Fiery RIP server that interfaces
to it for network printing). Cost: around $3000. for the 5000 only.
The 5000 is a much more robust printer than the 700 type Epson models. It
can print up to 13"X19" formats. It is faster than the 700 series and the
ink cartridges and paper capacity are much greater.

To realistically compare the printers I ran a speed and print quality
comparison between my two models. I printed a full 8 1/2" X 11" full color
bleed consisting of 3 x-ray maps, a BSE image and an EDS spectrum, along
with some text. Printing on the same PC, under the same conditions the print
quality is about the same. (A little disappointing here). However the print
speed was a full 4 times faster on the 5000 as compared to the 700. Actual
print time with the 5000 was about 2.5 min. at 1440 DPI.
I found an excellent web site which compares various Epson Photo printers,
including the 5000 and 700 series, and demonstrates print quality issues
quite vividly:

http://www.tssphoto.com/sp/dg/news/dot_comp.html

All in all I believe the 5000 is a much higher caliber printer than the 700
series, but unfortunately the print quality is about the same.

One final note: the paper makes ALL the difference in the world with this
class of printers.
Email me if you need info and suggestions on paper issues...



Chuck Burilla
United Technologies Research Center
400 Silver Lane
East Hartford, CT 06108

(860) 610-7388
burilact-at-utrc.utc.com



-----Original Message-----
} From: Malcolm Haswell [mailto:malcolm.haswell-at-sunderland.ac.uk]
Sent: Tuesday, February 15, 2000 9:03 AM
To: Microscopy (MSA)


I saw the Epson web spec and it looks quite impressive - especially as
they seem to be quoting speeds for real picture printing, not 5%
coverage or text.

My only concern is that Epson now market two printers with "10 years
light fastness" (the Epson Stylus photo 870 - A4 and 1270 A3) so you
have to wonder which is better for e.m. prints.

Malcolm Haswell
Electron Microscopy
School of Sciences
Fleming Building
University of Sunderland
SUNDERLAND SR1 3SD
Tyne and Wear
UK

Tel (0191) 515 2872
e-mail: malcolm.haswell-at-sunderland.ac.uk

---------------------------------------------------------

Tom Reese wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Epson has a new ink jet printer out-Stylus pro 5000-that is expensive
} but they claim it is much better than the much cheaper models many of
} us know and love. Would anyone know whether this is true of just
} hype-hard to tell from the published specs...? Thanks...Tom Reese



From daemon Wed Feb 16 20:58:31 2000



From: Paul Webster :      pwebster-at-mailhouse.hei.org
Date: 16 Feb 00 15:54:53 -0800
Subject: General:Wall Posters

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Hello,

Does anyone know the name of a supplier of wall posters suitable for an EM lab. I am thinking of cut-out diagrams of TEM and SEM, history of EM (I think JEOL once had one like that), specimen preparation etc.
Regards,

Paul Webster, Ph.D
House Ear Institute
2100 West Third Street
Los Angeles, CA 90057
phone:213 273 8026
fax: 213 413 6739
e-mail: pwebster-at-hei.org
http://www.hei.org/htm/aemi.htm



From daemon Wed Feb 16 20:58:35 2000



From: oshel-at-terracom.net (Philip Oshel)
Date: Wed, 16 Feb 2000 19:57:42 -0600
Subject: Gordon Cougar

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html
by ultra5.microscopy.com (8.9.1b+Sun/8.9.1) id UAA14463
for dist-Microscopy; Wed, 16 Feb 2000 20:00:35 -0600 (CST)
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Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"


My apologies to the list, but: could Gordon Cougar email me? I get a fatal
error when trying to email you:
The following addresses had permanent fatal errors -----
} {gcouger-at-rfdata.net}
[...]
} 550 {gcouger-at-rfdata.net} ... User unknown

Thanks!

Phil

*** Be famous! Send a Tech Tip or article to Microscopy Today! ***
Philip Oshel
Technical Editor, Microscopy Today
P.O. Box 620068
Middleton, WI 53562-0068
Voice: days (608) 263-4162, evening (608) 833-2885
Fax (608) 836-1969 (please make sure my name is on any fax)

Address for UPS, FedEx, or other couriers:
Dept. of Animal Health and Biomedical Sciences
University of Wisconsin
1656 Linden Drive
Madison, WI 53706-1581

oshel-at-terracom.net
or
peoshel-at-facstaff.wisc.edu




From daemon Thu Feb 17 16:22:03 2000



From: Steve D'Angelo :      steve-at-equiprx.net
Date: Thu, 17 Feb 2000 03:17:28 -0800
Subject: General:Wall Posters

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Paul,
My favorite has always been the DIATOME calendars.
Next are the Gatan calanders.
Dennis Kunkel has great original SEMs of diatomes etc, but I don't know if
he has any posters.
David Sharpe has the old standby insect SEMs.
The students at SanJoaquin Delta College have put together some good
calendars also.

} Date: 16 Feb 00 15:54:53 -0800
} From: Paul Webster {pwebster-at-mailhouse.hei.org}
} Subject: General:Wall Posters
} To: MSA listserver submission {Microscopy-at-sparc5.microscopy.com}
} X-Priority: 3
} MIME-Version: 1.0
} Reply-To: Paul Webster {pwebster-at-mailhouse.hei.org}
} X-MIME-Autoconverted: from quoted-printable to 8bit by
} ultra5.microscopy.com id RAA14282
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Very best regards,
Steve D'Angelo
Equipment Resurrection
650-738-0351

NOW at http://equiprx.net/




From daemon Thu Feb 17 01:30:04 2000



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Thu, 17 Feb 2000 16:14:42 GMT+1200
Subject: DP fluid for JEOL 840

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Hello, Pumpers

Does anyone out there have direct experience of any benefit(s) gained
by the use of Santovac 5 in the diffusion pumps of a JEOL 840,
instead of the recommended Lion S?

And did they have to fiddle with either the heating elements or
heater supply voltages in order to acheive the benefits?

I know that Santovac is well thought of, but it's expensive, and my
experience of Lion S in an older JEOL (JXA-5A) has been very
positive. I think there's something in there about optimising the
pump design to a particular fluid.

As an economical alternative, mentioned in Wil Bigelow's book, has
anyone tried NEOVAC SY, marketed by Varian, in an 840?

Does anyone know what chemical family Lion S belongs to?

Perhaps someone from JEOL, or even from the Lion company might care
to reply.

cheers

Ritchie

Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand



From daemon Thu Feb 17 20:21:56 2000



From: Gordon Vrololjak :      gvrdolja-at-nature.Berkeley.EDU
Date: Thu, 17 Feb 2000 17:04:35 -0800 (PST)
Subject: Mikros Manual available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,
I'm cleaning out some things in the laboratory and we have a Mikros Vacuum
Evaporator Manual C-20, 1964 here. We no longer have this evaporator, so
we no longer need the manual. If anyone would have need of it, please
email me personally and we'll send it out to you.

I'm amazed at the quality of this older manual - actual blue prints
included!

\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
Gordon Ante Vrdoljak Electron Microscope Lab
ICQ 23243541 http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
gvrdolja-at-nature.berkeley.edu UC Berkeley
phone (510) 642-2085 Berkeley CA 94720-3330
fax (510) 643-6207 cell (510) 290-6793



From daemon Fri Feb 18 20:56:58 2000



From: ard-at-ansto.gov.au (Arthur Day)
Date: Sat, 19 Feb 2000 09:58:59 +1100
Subject: Re: DP fluid for JEOL 840

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Ritchie,

There was a fairly extensive discussion about Dp oils on the listserver a
while ago. That should be in the archives somewhere.

}
} Does anyone out there have direct experience of any benefit(s) gained
} by the use of Santovac 5 in the diffusion pumps of a JEOL 840,
} instead of the recommended Lion S?
}

"Yes". We have been using Santovac 5 in our early 6400 machine for about 5
months now. The diffusion pumps appear to be the same as on an 840 and the
6400 had Lion S in it beforehand.

} And did they have to fiddle with either the heating elements or
} heater supply voltages in order to acheive the benefits?
}

No. The vacuum seems to be as good as ever without doing any of this. It
all looks very clean.

} I know that Santovac is well thought of, but it's expensive, and my
} experience of Lion S in an older JEOL (JXA-5A) has been very
} positive. I think there's something in there about optimising the
} pump design to a particular fluid.
}
} As an economical alternative, mentioned in Wil Bigelow's book, has
} anyone tried NEOVAC SY, marketed by Varian, in an 840?
}
} Does anyone know what chemical family Lion S belongs to?

That should all be in the archives somewhere, or perhaps on certain web
sites. Jim Darley and/or Will Bigelow had the answers.

}
} Perhaps someone from JEOL, or even from the Lion company might care
} to reply.
}

We were told that the Santovac 5 is more tolerant of the occasional gulp of
air.....so presumably it should last longer.

Cheers


Arthur Day, Electron Microscope Unit Phone: 61-2-9717-3457
Ansto Materials Division Fax: 61-2-9543-7179
PMB 1, Menai (Sydney), NSW, 2234 Email: ard-at-ansto.gov.au
Australia www: http://www.ansto.gov.au/




From daemon Fri Feb 18 20:56:57 2000



From: bozzola-at-siu.edu (John J. Bozzola)
Date: Fri, 18 Feb 2000 17:59:22 -0600
Subject: Photoshop 5.5 Thumbnail Preview in Macs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


For imaging gurus:

I am using Photoshop 5.5 on a G3 Macintosh and can no longer get a preview
of the image when the file is selected in FILE } OPEN. What gives?

In previous versions (say 4.0 and earlier) when the file was selected, I
got a thumbnail preview of the image prior to opening it. This is very
convenient and I miss this capability.

Thanks,

John

####################################################################
John J. Bozzola, Ph.D., Director
Micro-Imaging and Analysis Center
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
####################################################################




From daemon Sat Feb 19 13:13:31 2000



From: steve :      halifa-at-globalnet.co.uk
Date: Sat, 19 Feb 2000 12:32:11 -0600
Subject: Re Santovac

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Santovac is a great oil to use in Jeol instruments. Backstreaming is
less and it will withstand a lot more abuse than Lion oil if the vac
system has a glitch. Just put a little less charge in than the normal Lion
oil. Regards Steve Parkins




From daemon Sat Feb 19 13:13:30 2000



From: Wright, Cheryl W :      CWright-at-Sikorsky.com
Date: Sat, 19 Feb 2000 12:32:33 -0600
Subject: Photography of Coated Replicas

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Everyone,

WOW, thanks soooo much for all the responses to my question. I have alot of
techniques to try so that should keep me busy for awhile. This is a very
informative site and I appreciate the help!!

Cheryl




From daemon Sun Feb 20 18:03:29 2000



From: Dean Abel :      dean-abel-at-uiowa.edu
Date: Sun, 20 Feb 2000 14:48:35 -0600
Subject: TEM Philips 300

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The Department of Biological Sciences at the University of Iowa must find a
home for a Philips 300 Transmission Electron Microscope by June 2000. It
is in excellent condition and has been under service contract for nearly 30
years. We are remodeling our building and will no longer maintain support
for this instrument. Free to anyone willing to come and take it away (plus
Haskris chiller, carbon evaporator, film dessicator, etc.).

Please contact:
Dean Abel
Biological Sciences 138BB
University of Iowa
Iowa City IA 52242 USA
319-335-1070 (fax 319-335-1069)
dean-abel-at-uiowa.edu



From daemon Sun Feb 20 18:03:30 2000



From: Diana van Driel :      dianavd-at-eye.usyd.edu.au
Date: Mon, 21 Feb 2000 09:49:50 +1100
Subject: Photoshop 5.5 Thumbnail Preview in Macs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear John,

Open the image in Photoshop and select SAVE AS. There are boxes to be
ticked, one of which is Mac thumbnail. My guess is that your problem
is here.

Diana



Diana van Driel
Department of Clinical Ophthalmology
Sydney University
GPO Box 4337
Sydney NSW
AUSTRALIA 2001

Phone 61 2 938 27278/27395
Mob 0412 165 075
Fax 61 2 938 27318



From daemon Mon Feb 21 15:05:39 2000



From: Robert Champaign :      r-champaign-at-raytheon.com
Date: Mon, 21 Feb 2000 08:05:00 -0800
Subject: Cool Prep (Water Coolers)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We are currently using Cool Prep in our Coolwell cooler system. This is a
liquid cleaner & slime preventer that helps keep mineral deposits to a
minimum in the cooling system. It also helps prevent corrosion in the
water passages without harming hoses, gaskets or seals. Since Coolwell is
no longer in business does anyone know of a replacement product for Cool
Prep.

Can we simply switch to ethylene glycol?
Robert Champaign
Raytheon
Electronic Systems Labs - Texas Region
r-champaign-at-raytheon.com
2501 W.University, MS 8011
McKinney Texas, 75070
972-952-3165



From daemon Mon Feb 21 15:05:41 2000



From: Michael Coviello :      coviello-at-mae.uta.edu
Date: Mon, 21 Feb 2000 09:58:54 -0600
Subject: SEM-JEOL 845 TMP problems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Y'all:
We have an JEOL 845 SEM (TMP pumped) and we have been having problems with the
TMP REV light staying on. Because of the microscopes' timing circuit, the
scope will shut off after 20 minutes. The shutting off/timing issue can be
overridden by keeping the key turned clockwise: the microscope will
eventually go to the correct vacuum, but the darn TMP light never goes out.
JEOL told us of a trick of depressing the standby button on the TMP
controller, sometimes remedies this situation, but it didn't work in our
case. We have been in contact with JEOL and so far we/they haven't figured
out the problem. The TMP pump and the rough pump both seem OK: the TMP 80 %
relay kicks in at 80% RPM as it's supposed to and we changed the rough pump
oil, just to be sure). I have a few questions to ask of you 845 owners:
1) Have you seen this type of problem, and if so, how did you remedy it?
2) Does anyone out there have wiring diagrams, manuals for the TMP 845 (not
the DP, we already have that), if so can we get a copy? JEOL doesn't seem to
have anything.

Your help will be greatly appreciated.
Mike Coviello
Lab Manager
UT Arlington
Arlington, TX
817 272-5496



From daemon Mon Feb 21 15:05:47 2000



From: Ziel, R. (Rainer) :      Rainer.Ziel-at-akzonobel.com
Date: Mon, 21 Feb 2000 19:55:17 +0100
Subject: LM: Video-Adapter for Zeiss Photo Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have an old Photomicroscope from Zeiss. It's grey in color and about 20
years old, but works pretty good. Until now we have an old 1 inch video
camera on top. We want to change this video camera to a state of art
CCD-camera with 1/2 inch CCD-chip. To enlarge the field of view, we want to
use a video adapter with a magnification 0.5x. Unfortunately several
oprtical corrections are made within this adapter. Such an adapter is not
offered by Zeiss. Does anybody out there knows a company that sells such a
special adapter or can build one?

Kind Regards

Rainer

--------

Rainer Ziel
Acordis Analytical Services Obernburg
63784 Obernburg
Germany
Phone: 06022/812645
email: rainer.ziel-at-acordis.com





From daemon Mon Feb 21 15:05:47 2000



From: Ziel, R. (Rainer) :      Rainer.Ziel-at-akzonobel.com
Date: Mon, 21 Feb 2000 19:55:17 +0100
Subject: LM: Video-Adapter for Zeiss Photo Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have an old Photomicroscope from Zeiss. It's grey in color and about 20
years old, but works pretty good. Until now we have an old 1 inch video
camera on top. We want to change this video camera to a state of art
CCD-camera with 1/2 inch CCD-chip. To enlarge the field of view, we want to
use a video adapter with a magnification 0.5x. Unfortunately several
oprtical corrections are made within this adapter. Such an adapter is not
offered by Zeiss. Does anybody out there knows a company that sells such a
special adapter or can build one?

Kind Regards

Rainer

--------

Rainer Ziel
Acordis Analytical Services Obernburg
63784 Obernburg
Germany
Phone: 06022/812645
email: rainer.ziel-at-acordis.com





From daemon Mon Feb 21 15:47:03 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Mon, 21 Feb 2000 15:10:21 -0600
Subject: Administrivia: Server is acting strange...

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Colleagues...

Yes I know the server appears to be acting up (i.e. not sending
any mail out).. I'm looking into the problem.

Nestor
Your Friendly Neighborhood SysOp




From daemon Mon Feb 21 17:08:33 2000



From: bozzola-at-siu.edu (John J. Bozzola)
Date: Mon, 21 Feb 2000 16:59:58 -0600
Subject: LM: COURSES

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


FORWARDED TO LISTSERVER,
PLEASE RESPOND TO THE ADDRESS SHOWN BELOW:

{randy_anderson-at-ameritech.net}

I am a member of MSA and I work for the University of Chicago in the
section of Nephrology. I was wondering if you would know of any
universities or other types of institutions that would offer summer
courses for the beginner and advance areas of immunofluorescence
microscopy. Any help that you can give me in matter would greatly be
appreciated.

Thank you

Randy Anderson

####################################################################
John J. Bozzola, Ph.D., Director
Micro-Imaging and Analysis Center
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
####################################################################




From daemon Mon Feb 21 17:08:34 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Mon, 21 Feb 2000 17:07:02 -0600
Subject: Testing using TestList

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


This is a test to 3 zaluzec addresses and the archive.
5:06 pm




From daemon Mon Feb 21 17:25:15 2000



From: Earl Weltmer :      earlw-at-pacbell.net
Date: Mon, 21 Feb 2000 15:17:47 -0800
Subject: Re: SEM-JEOL 845 TMP problems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have a copy can send you.

The JEOL Schematics are somewhat ambiguous as the logic from the turbo pump
activates several relays. The relays are NOT connected in the manner that one
would think, i.e. when the turbo pump speed is 80% the relay is activated and
signals the vacuum logic that all is well.

Earl Weltmer

Michael Coviello wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hi Y'all:
} We have an JEOL 845 SEM (TMP pumped) and we have been having problems with the
} TMP REV light staying on. Because of the microscopes' timing circuit, the
} scope will shut off after 20 minutes. The shutting off/timing issue can be
} overridden by keeping the key turned clockwise: the microscope will
} eventually go to the correct vacuum, but the darn TMP light never goes out.
} JEOL told us of a trick of depressing the standby button on the TMP
} controller, sometimes remedies this situation, but it didn't work in our
} case. We have been in contact with JEOL and so far we/they haven't figured
} out the problem. The TMP pump and the rough pump both seem OK: the TMP 80 %
} relay kicks in at 80% RPM as it's supposed to and we changed the rough pump
} oil, just to be sure). I have a few questions to ask of you 845 owners:
} 1) Have you seen this type of problem, and if so, how did you remedy it?
} 2) Does anyone out there have wiring diagrams, manuals for the TMP 845 (not
} the DP, we already have that), if so can we get a copy? JEOL doesn't seem to
} have anything.
}
} Your help will be greatly appreciated.
} Mike Coviello
} Lab Manager
} UT Arlington
} Arlington, TX
} 817 272-5496



From daemon Mon Feb 21 18:20:00 2000



From: Christoph Bauer :      cbauer-at-midway.uchicago.edu
Date: Mon, 21 Feb 2000 17:33:42 -0800
Subject: Position available: EM-TECHNICIAN

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


ELECTRON MICROSCOPY TECHNICIAN
A full time position for a senior research technician is available at
the University of Chicago in the Department of Molecular Genetics and
Cell Biology. The lab of Elaine Fuchs Ph.D. is interested in the
cytoskeleton, in cell-cell adhesion and in the cell biology of skin
development.
The successful candidate will perform routine transmission electron
microcopy, including specimen acquisition, tissue processing,
ultramicrotomy, operation of a Philips CM120 electron microscope and
darkroom procedures/digital imaging. In addition he/she will be trained
in on-section immunolabeling using Lowicryl K4M.
Applicant Qualifications: Minimum requirements are a Bachelors degree
and 2 years of relevant experience.
If you are motivated to work in an excellent scientific environment with
state of the art instrumentation please contact:

Christoph Bauer Ph.D.
University of Chicago,
Department of Molecular Genetics and Cell Biology
5841 S. Maryland Ave/MC 1028
Chicago, Il 60637

Phone: 773 834 2896
Fax: 773 702 0141
Email:cbauer-at-midway.uchicago.edu



From daemon Mon Feb 21 18:31:28 2000



From: Robert Dourmashkin :      robert-at-bobsydnee.demon.co.uk
Date: Mon, 21 Feb 2000 18:18:47 -0600
Subject: MT2 Microtome

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Does anybody need a MT2 Porter-Blum ultramicrotome, in running order but
no binocular? Yours for the asking and transport.

Robert Dourmashkin,
Section of Academic Virology,
Royal London School of Medicine,
40 Turner Street,
Whitechapel London E1 2AD.
--
Robert Dourmashkin




From daemon Mon Feb 21 18:31:28 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Mon, 21 Feb 2000 18:16:54 -0600
Subject: Administrivia: Restarting the Server

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Colleagues...

I'm reinitializing the server. You may see some old and/or
duplicate messages. I will try to restore those that I think
did not get through. If you tried to post a message in the last
day or so and haven't seen it please wait ~ 12 hours after
that time if you do not see it appearby then , you should
presume it was lost and repost your original
question/comment.

Sorry...

Nestor
Your Friendly Neighborhood SysOp




From daemon Mon Feb 21 18:31:29 2000



From: Greg Erdos :      gwe-at-biotech.ufl.edu
Date: Mon, 21 Feb 2000 18:18:12 -0600
Subject: Fwd: SEM/TEM Course

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




} } Ms. Lalini Pillayi Would like to take both a TEM and SEM short
} } course. She is located at the at the US ARmy Dental Research Great
} } Lakes Lab.
} } Her applications will be both biological and biological
} } combined with material. If you offer such a course please contact her
} } directly at
} } lalini70-at-hotmail.com
} }
} } Thanks

Gregory W. Erdos, Ph.D. Ph. 352-392-1295
Assistant Director, Biotechnology Program
PO Box 110580 Fax:
352-846-0251
University of Florida
Gainesville, FL 32611






From daemon Mon Feb 21 18:31:29 2000



From: Earl Weltmer :      earlw-at-pacbell.net
Date: Mon, 21 Feb 2000 18:17:32 -0600
Subject: Re: SEM-JEOL 845 TMP problems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




I have a copy can send you.

The JEOL Schematics are somewhat ambiguous as the logic from the turbo pump
activates several relays. The relays are NOT connected in the manner that one
would think, i.e. when the turbo pump speed is 80% the relay is activated and
signals the vacuum logic that all is well.

Earl Weltmer

Michael Coviello wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hi Y'all:
} We have an JEOL 845 SEM (TMP pumped) and we have been having problems
} with the
} TMP REV light staying on. Because of the microscopes' timing circuit, the
} scope will shut off after 20 minutes. The shutting off/timing issue can be
} overridden by keeping the key turned clockwise: the microscope will
} eventually go to the correct vacuum, but the darn TMP light never goes out.
} JEOL told us of a trick of depressing the standby button on the TMP
} controller, sometimes remedies this situation, but it didn't work in our
} case. We have been in contact with JEOL and so far we/they haven't figured
} out the problem. The TMP pump and the rough pump both seem OK: the TMP 80 %
} relay kicks in at 80% RPM as it's supposed to and we changed the rough pump
} oil, just to be sure). I have a few questions to ask of you 845 owners:
} 1) Have you seen this type of problem, and if so, how did you remedy it?
} 2) Does anyone out there have wiring diagrams, manuals for the TMP 845 (not
} the DP, we already have that), if so can we get a copy? JEOL doesn't seem to
} have anything.
}
} Your help will be greatly appreciated.
} Mike Coviello
} Lab Manager
} UT Arlington
} Arlington, TX
} 817 272-5496




From daemon Mon Feb 21 18:31:29 2000



From: Fred Pearson :      eoptics-at-mcmail.cis.mcmaster.ca
Date: Mon, 21 Feb 2000 18:18:24 -0600
Subject: Kevex Preamp Manual

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Good morning:

We would like to resurrect 2 older Kevex SiLi detectors for scintillation
counting, and have lost or misplaced the manuals for the preamp output
connectors, ie. voltages and pinouts.
The model numbers of the preamps are 2002 and 2003.

If anyone has this info, could you please fax or email?

Thanks in advance

Fred Pearson

********************************************************
Fred Pearson
Brockhouse Institute for Materials Research
McMaster University
1280 Main St. West
Hamilton, Ontario
Canada L8S 4M1


email: eoptics-at-mcmaster.ca
phone: (905) 525-9140 ext. 24609
fax: (905) 521-2773
********************************************************




From daemon Mon Feb 21 18:31:30 2000



From: Christoph Bauer :      cbauer-at-midway.uchicago.edu
Date: Mon, 21 Feb 2000 18:21:28 -0600
Subject: Position available: EM-TECHNICIAN

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



ELECTRON MICROSCOPY TECHNICIAN
A full time position for a senior research technician is available at
the University of Chicago in the Department of Molecular Genetics and
Cell Biology. The lab of Elaine Fuchs Ph.D. is interested in the
cytoskeleton, in cell-cell adhesion and in the cell biology of skin
development.
The successful candidate will perform routine transmission electron
microcopy, including specimen acquisition, tissue processing,
ultramicrotomy, operation of a Philips CM120 electron microscope and
darkroom procedures/digital imaging. In addition he/she will be trained
in on-section immunolabeling using Lowicryl K4M.
Applicant Qualifications: Minimum requirements are a Bachelors degree
and 2 years of relevant experience.
If you are motivated to work in an excellent scientific environment with
state of the art instrumentation please contact:

Christoph Bauer Ph.D.
University of Chicago,
Department of Molecular Genetics and Cell Biology
5841 S. Maryland Ave/MC 1028
Chicago, Il 60637

Phone: 773 834 2896
Fax: 773 702 0141
Email:cbauer-at-midway.uchicago.edu




From daemon Mon Feb 21 18:31:30 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Mon, 21 Feb 2000 18:22:34 -0600
Subject: JEOL 845 SEM (TMP Light)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hi Y'all:
We have an JEOL 845 SEM (TMP pumped) and we have been having problems with the
TMP REV light staying on. Because of the microscopes' timing circuit, the
scope will shut off after 20 minutes. The shutting off/timing issue can be
overridden by keeping the key turned clockwise: the microscope will
eventually go to the correct vacuum, but the darn TMP light never goes out.
JEOL told us of a trick of depressing the standby button on the TMP
controller, sometimes remedies this situation, but it didn't work in our
case. We have been in contact with JEOL and so far we/they haven't figured
out the problem. The TMP pump and the rough pump both seem OK: the TMP 80 %
relay kicks in at 80% RPM as it's supposed to and we changed the rough pump
oil, just to be sure). I have a few questions to ask of you 845 owners:
1) Have you seen this type of problem, and if so, how did you remedy it?
2) Does anyone out there have wiring diagrams, manuals for the TMP 845 (not
the DP, we already have that), if so can we get a copy? JEOL doesn't seem to
have anything.

Your help will be greatly appreciated.
Mike Coviello
Lab Manager
UT Arlington
Arlington, TX
817 272-5496




From daemon Mon Feb 21 18:35:34 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Mon, 21 Feb 2000 18:23:47 -0600
Subject: Prep of rat brains for TEM using vascular perfusion

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Heather A Owen wrote:
}
} Hello everyone,
}
} A graduate student is getting ready for a rather large scale preparation
} of rat brains for TEM using vascular perfusion. Luckily (since I'm a
} botanist) her major professor has had experience with the procedure. He
} doesn't know for sure whether he used EM or Biological grade
} glutaraldehyde previously, but considering the large volume that will be
} required, and the cost difference, I'm hoping that the Biological grade
} will suffice.
}
} I would appreciate advice from those of you that have experience with this
} method of fixation.
}
} Thank you in advance for any information.
}
} Heather Owen
}
} Heather A. Owen, Director
} Electron Microscope Laboratory
} Department of Biological Sciences
} University of Wisconsin - Milwaukee
} (414)229-6816

Heather,

It's been a while but, back when I did such things, I had bouts of
non-uniform fixation when using biological grade glutaraldehyde solutions
even in plant tissues. However, the solutions had been kept much longer than
I'd now use and there are other preparation technique changes that confound
the determination of causality. In the cases where I've perfused small
animals, I've used EM grade GA since, as expensive as it is, it's cheaper
than repeating the experiment.

However, I did a full systemic perfusion. One random thought ( I haven't
tried it): What's the vascular volume of a rat brain? If you just need just
the brain, I'd think a little creative clamping (really small hemostats?) in
the thorax should let you reduce the volume needed to make even a large
experiment affordable with "the good stuff".

cheers and good luck,
John

John Heckman
MSM Department
Michigan State University





From daemon Mon Feb 21 18:35:42 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Mon, 21 Feb 2000 18:24:19 -0600
Subject: OIL for use in JEOL's

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Santovac is a great oil to use in Jeol instruments. Backstreaming is
less and it will withstand a lot more abuse than Lion oil if the vac
system has a glitch. Just put a little less charge in than the normal Lion
oil. Regards Steve Parkins






From daemon Mon Feb 21 18:35:48 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Mon, 21 Feb 2000 18:25:09 -0600
Subject: More on Rat Brain Prep..

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



We use Biological grade to perfuse rats (whole body, we haven't tried clamping
bits off, though that might be a good idea since we're interested in just the
head). After the perfusion is finished, we remove the interesting bit, which is
huge by EM standards, and fix some more in Karnowski's. Then we go on to
osmium,
and continue processing. We get acceptable EM pictures at high magnification,
even though our blocks are so large and processing times are therefore
extended. So I can recommend Biological grade glutaraldehyde. Hope this helps.

Lesley Weston.





From daemon Mon Feb 21 18:35:55 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Mon, 21 Feb 2000 18:25:57 -0600
Subject: CCD Camera on Photomicroscope from Zeiss

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



We have an old Photomicroscope from Zeiss. It's grey in color and about 20
years old, but works pretty good. Until now we have an old 1 inch video
camera on top. We want to change this video camera to a state of art
CCD-camera with 1/2 inch CCD-chip. To enlarge the field of view, we want to
use a video adapter with a magnification 0.5x. Unfortunately several
oprtical corrections are made within this adapter. Such an adapter is not
offered by Zeiss. Does anybody out there knows a company that sells such a
special adapter or can build one?

Kind Regards

Rainer

--------

Rainer Ziel
Acordis Analytical Services Obernburg
63784 Obernburg
Germany
Phone: 06022/812645
email: rainer.ziel-at-acordis.com





From daemon Mon Feb 21 18:36:19 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Mon, 21 Feb 2000 18:26:40 -0600
Subject: geometry settings for a Kevex Quantum detector for JEOL 2000FX

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Does anyone have the geometry settings for a Kevex Quantum detector on a
JEOL 2000FX? I am using DTSA and need the values. I would like to have the
sample to detector distance and the azimuthal angle. I am using +45 for the
azimuthal angle and 90 for the detector angle. Are these correct? I know
that the takeoff angle is 70.
-Scott


Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)





From daemon Mon Feb 21 18:36:40 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Mon, 21 Feb 2000 18:27:29 -0600
Subject: looking for used ion mill

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hello microscopy listserver-type people,

I am in the market for a used ion mill. Can anybody point me to one?

Thanks,
Larry

--
------------------------------------------------------------------------------
Larry R. Nittler Laboratory for Extraterrestrial Physics,
Code 691
Interstellar Dust Buster NASA Goddard Spaceflight Center
Greenbelt MD 20771
phone: 301-286-4572 fax:301-286-0212
nittler-at-lepvax.gsfc.nasa.gov http://www.ciw.edu/lrn/
------------------------------------------------------------------------------




From daemon Mon Feb 21 18:46:00 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Mon, 21 Feb 2000 18:28:06 -0600
Subject: RE: autofluorescence quenching:spectral imaging can help

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} From: RLevenson-at-cri-inc.com Subject: RE: autofluorescence quenching:
spectral imaging can help

You asked about strategies for reducing autofluorescence.

In addition to various strategies which can include using near-IR dyes like
Cy5.5 and exciting far in the red, you can also treat tissues with sodium
borohydride or toluidine blue (these latter observations I pass on from
others--have not tried them myself).

I have recently had very promising results using spectral imaging to
discriminate autofluorescence from desired fluorescence. [Caution: I am
describing a product sold by my company]. CRI makes a liquid crystal
tunable filter (LCTF) that can be used to collect a stack of images at
different wavelengths. This yields a spectrum at every pixel of an image.
(A product with similar capabilities is sold by Applied Spectral Imaging,
but uses a different technology.) CRI's new LCTF, the FluoroSpec, uses
special optics to combine polarization states; along with other
improvements, it has more than twice the light throughput compared to
previous models.

The sample was formalin-fixed brain probed with a Cy2-labeled antibody to a
low-abundance protein. By eye it was very difficult to distinguish the Cy2
signal from the bright green to green-orange autofluorescence. Using a
spectral data set (15 images from 510 nm to 650 nm at 10 nm intervals) and
linear combination algorithms for pixel-unmixing, it was possible to
completely separate the Cy2 and autofluorescence images.

I would be glad to share these images with any interested parties.

Richard


Richard Levenson, MD
Technical Director, Biomedical Systems
Cambridge Research & Instrumentation (CRI), Inc.
80 Ashford St.
Boston, MA 02134

Ph: (617) 787-5700; Fax: (617) 787-4488
rlevenson-at-cri-inc.com www.cri-inc.com




From daemon Mon Feb 21 18:46:08 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Mon, 21 Feb 2000 18:29:00 -0600
Subject: Help on fluorescence

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Hi all. I have just joined this list and am posting without the usual
"lurking time" due to time constraints - please forgive if recently
covered. I am also a novice in all microscopy techniques, especially
fluorescence.

I am trying to detect GFP-containing cells in liver tissue and having
some problems.

1. I can't determine the spectra for the filter blocks I am using (on a
Nikon Eclipse TE300 with TE-FM Epifluorescence attachment). The blocks
are marked "UV", "B-2" (blue from source, green in field) and "G" (green
from source, red in field). I have e-mailed Nikon and to be fair
they've only had a few days but I'm under some pressure. No help from
the manual. I have been using B-2 for GFP.

2. I get quite a lot of background fluorescence even with frozen
sections (fixed in neutral buffered formalin). Can anyone suggest a way
to reduce this? (eg any extra filters?)

3. I have read conflicting opinions on fixation. Most previous work has
used thick sections (50 microns cut eg with Vibratome, ? to avoid having
to embed tissue blocks). GFP is interfered with by acetone (and probably
other organic solvents) but I would have thought that after fixation
with formalin it should be stabilized and resistant to the
xylene/alcohol used in paraffin embedding. I have seen fluorescence
retained after this treatment but perhaps it can be improved.

4. For those with liver fluoro experience - on "UV" setting I see bright
fluorescence which photobleaches. I believe I am looking at retinoids in
stellate cells, although the bleaching is incomplete and a bit slower
than I have seen before. Can anyone confirm this?

Apologies for long post. Any help much appreciated.

David Lockwood
University of Qld Dept of Surgery
PA Hospital Brisbane Australia





From daemon Mon Feb 21 19:02:28 2000



From: A. Greene :      ablue-at-io.com
Date: Monday, February 21, 2000 10:02 AM
Subject: Cool Prep (Water Coolers)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Robert,
Preparation of the water in water chillers is a strange world of its own and
you may get many responses to your question. The majority of my experience
has been with the Haskris systems but the chemistry may be the same. In
about 30 years of looking after many chillers, my formula for success goes
like this:
1 - Use deionized or distilled water. The water will leach enough products
from the hoses, microscope lenses, pumps and so forth to reach a equilibrium
and then be "ideally suited" for your system.
2 - Don't use any fungicide or other bug killer because it will slime up the
works.
3 - Put 4 or 5 drops of oil on top of the water reservoir. This will form a
barrier by creating a monolayer on top of the water and keep things from
growing in the water.
I have used this proceedure in tropical climates and in the north and in
both dry and humid environments and it has always worked very well. I can
almost gurantee you that ethyline glycol will gum up the works.

Best wishes and good luck.

Alex Greene
SCIENTIFIC INSTRUMENTATION SERVICES, INC.
PMB-499, 1807 West Slaughter Lane, Number 200
Austin, Texas 78748-6200 Phone:512-282-5507 FAX 512/280-0702

QUALITY ELECTRON MICROSCOPE REPAIRS


-----Original Message-----
} From: Robert Champaign {r-champaign-at-raytheon.com}
To: Microscopy-at-sparc5.microscopy.com {Microscopy-at-sparc5.microscopy.com}



From daemon Mon Feb 21 23:39:42 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Mon, 21 Feb 2000 23:38:16 -0600
Subject: looking for a bad address

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



This is a short test

11:38 pm




From daemon Mon Feb 21 23:48:26 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Tue, 22 Feb 2000 00:21:26 -0600
Subject: another test but to microscopy@sparc5.microscopy.com

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


another test but to microscopy-at-sparc5.microscopy.com




From daemon Tue Feb 22 03:55:33 2000



From: Jo Verbeeck :      joverbee-at-ruca.ua.ac.be
Date: Tue, 22 Feb 2000 10:52:37 +0100 (MET)
Subject: TEM EELS:How to focus ESI images?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello all,

I'm using a CM30 FEG with GIF PEELS atached to it. I'm getting confused
about focussing an energy filtered image.

GATAN proposes to focus the image on the TV camere at a loss of
100eV.Apparently it will be focused more or less OK at a higher loss say
741eV.
DM uses the voltage offset which changes the HT of the microscope. So as
far as I understand the focus should be OK for every energy loss(no
refocussing should be needed at all) . Although in practice it is not. The
trick with focussing at 100eV works better than not focussing at all but
it is not satisfactory for high spatial resolution.

In the Phd of M.Schenner (TU Wien "The quantification problem in EELS
Microanalysis") I found a callibration procedure to find a defocus to
correct for chromatic abberation as a function of the energy loss you are
interested in. But he seems to use the drift tube in the GIF.

The question now: How should I focus my ESI images and what is the
explanation behind it?

Thanks in advance,

Jo

*************************************************************
* Jo Verbeeck *
* University of Antwerp *
* Dept. EMAT (Electron Microscopy for Materials Research) *
* e-mail: joverbee-at-ruca.ua.ac.be *
* tel: +32(0)3 218 02 49 *
* fax: +32(0)3 218 02 57 *
*************************************************************



From daemon Tue Feb 22 05:55:15 2000



From: ALEX BLACK :      ALEXANDER.BLACK-at-NUIGALWAY.IE
Date: Tue, 22 Feb 2000 11:29:17 +0000 (GMT)
Subject: Quinacrine fluorescence

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear All,
We are considering trying to label aspects of innervation using a
quinacrine fluorescence technique. So far the preliminary trials have not gone
well. Could anyone give advice on this, especially on a source for a reliable
fluorescent quinacrine dye?


Thanks in advance

Alex Black
Department of Anatomy
National University of Ireland, Galway



From daemon Tue Feb 22 05:45:16 2000



From: Barbara Foster :      mme-at-map.com
Date: Tue, 22 Feb 2000 08:11:57 -0500
Subject: Re: LM: COURSES

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Randy,

We have specialists in this area who can offer customized, on-site courses,
using your equipment and speaking specifically to your application. If you
are interested in further details, or in how U. Chi can sponsor such a
course, please contact me directly.

Best regards,
Barbara Foster
Consortium President
Microscopy/Microscopy Education ...Educating microscopists for greater
productivity.

125 Paridon Street Suite 102 Springfield, MA 01118
PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com
Visit our web site {http://www.MME-Microscopy.com/education}


At 04:59 PM 2/21/00 -0600, John J. Bozzola wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Tue Feb 22 07:25:03 2000



From: Barbara Foster :      mme-at-map.com
Date: Tue, 22 Feb 2000 08:16:36 -0500
Subject: RE: autofluorescence quenching:spectral imaging can help

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From daemon Tue Feb 22 07:55:03 2000



From: Debby Sherman :      sherman-at-btny.purdue.edu
Date: Monday, February 21, 2000
Subject: Re: Prep of rat brains for TEM using vascular perfusion

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I perfused dozens of rat brains over the years. I would recomend using the purified glutaraldehyde. However, you can use a slightly less concentrated solution since the fixation is so much more efficient tham immersion fixation. We primarily fixed for ICC but only the choice of fix would vary not the method. For normal ultrastructure, I would try 2-3% Glut or half-strength Karnovsky's in a phosphate buffer system with added NaCl and Mg at physiological concentrations and see which fixation I prefered by examining the desired tissues prior to fixing critical animals.

We would anesthetize the animal, open the chest cavity and clamp off the descending aorta, thus limiting the perfusion to the upper half of the animal. The cannula would then be inserted into a small slit in the left ventricle, positioned up into the aorta, and held with a hemostat. Perfusion lines should have been filled with normal saline or ringers so that as soon as the cannula is in place, the flow can be started to wash out the blood. A small slit is made in the right atrium to permit release of venous blood, etc. As soon as the return is relatively pale, switch to your fixative and run the desired amount through. The condition of the liver is a reasonable criteria for the efficiency of the perfusion. It should be very pale yellow as the blood is removed and should stiffen up within a few minutes. In fact the entire upper part of the animal should become quite stiff as the tissues are fixed. Figure on a couple of hundred mls. of fix per animal.

A couple of suggestions are to use the initial ringer wash and fixatives at room temperature or slightly above so as not to cause vessels to contract. Do any further washes and post fix the portions of interest at 4oC. Also make sure you have a consistant but gental perfusion rate so as not to break delicate capillaries. Usually this can be accomplished just by raising the vessels containing the buffer and fix above the animal and let gravity do the rest.
Good luck,
Debby

Debby Sherman, Manager Phone: 765-494-6666
Microscopy Center in Agriculture FAX: 765-494-5896
Dept. of Botany & Plant Pathology E-mail: sherman-at-btny.purdue.edu
Purdue University
1057 Whistler Building
West Lafayette, IN 47907-1057

--------------------------------------



Heather A Owen wrote:
}
} Hello everyone,
}
} A graduate student is getting ready for a rather large scale preparation
} of rat brains for TEM using vascular perfusion. Luckily (since I'm a
} botanist) her major professor has had experience with the procedure. He
} doesn't know for sure whether he used EM or Biological grade
} glutaraldehyde previously, but considering the large volume that will be
} required, and the cost difference, I'm hoping that the Biological grade
} will suffice.
}
} I would appreciate advice from those of you that have experience with this
} method of fixation.
}
} Thank you in advance for any information.
}
} Heather Owen
}
} Heather A. Owen, Director
} Electron Microscope Laboratory
} Department of Biological Sciences
} University of Wisconsin - Milwaukee
} (414)229-6816

Heather,

It's been a while but, back when I did such things, I had bouts of
non-uniform fixation when using biological grade glutaraldehyde solutions
even in plant tissues. However, the solutions had been kept much longer than
I'd now use and there are other preparation technique changes that confound
the determination of causality. In the cases where I've perfused small
animals, I've used EM grade GA since, as expensive as it is, it's cheaper
than repeating the experiment.

However, I did a full systemic perfusion. One random thought ( I haven't
tried it): What's the vascular volume of a rat brain? If you just need just
the brain, I'd think a little creative clamping (really small hemostats?) in
the thorax should let you reduce the volume needed to make even a large
experiment affordable with "the good stuff".

cheers and good luck,
John

John Heckman
MSM Department
Michigan State University








From daemon Tue Feb 22 07:55:03 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Tue, 22 Feb 2000 07:38:34 -0600
Subject: Administrivia: Still debugging

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Folks...

Still in a debug mode this is a very peculiar problem.
This test is going to the entire distribution..

Sorry

Nestor




From daemon Tue Feb 22 18:43:39 2000



From: John Catino :      jwcatino-at-concentric.net
Date: Tue, 22 Feb 2000 19:42:06 -0500
Subject: Enlarger and Print Processor Sale

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I have a Beseler 45M Enlarger and Kodak Royal Print Processor for sale.
Both are in working condition. We are going digital! Equipment is
located in Easton, PA.




John Catino




From daemon Tue Feb 22 18:43:39 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Tue, 22 Feb 2000 18:42:08 -0600
Subject: another test of Microscopy

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This is a second test without the 15 minute delay/queus




From daemon Tue Feb 22 18:48:47 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Tue, 22 Feb 2000 18:47:07 -0600
Subject: Testing at 6:47

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Testing at 6:47 to Microscopy




From daemon Tue Feb 22 19:38:42 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Tue, 22 Feb 2000 19:20:37 -0600
Subject: Administrivia: DNS restored (I think)

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Colleagues....

Well the DNS (Domain Name Server) failed and was probably
pretty sick over most of last week. Alot of mail probably just
ended up in a black hole somewhere on the Net. Some of you
may have gotten messages, but I see that most of the mail
I sent out during my trip DownUnder to the ACEM-16 meeting
in Canberra never made it to it's destination.

The DNS "looks" fixed now but I'm not confident enough to proclaim
all as back to normal yet. Let's see if this message actually
gets through to everyone this time.

Sorry gang....

Nestor
Your Friendly (Frustrated) Neighborhood SysOp




From daemon Tue Feb 22 21:30:38 2000



From: Toby Knight :      tknight-at-waite.adelaide.edu.au
Date: Wed, 23 Feb 2000 13:32:59 +1030 (CST)
Subject: Sudan Black staining and fixing

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Greetings,

I have 2 queries pertaining to the use of the lipid colourant Sudan Black
B, I hope someone can be of some help:

1. I have been able to stain oil traces in the glands of citrus fruit BUT
the epoxy resin Procure/Araldite also stains quite strongly. Is this
typical of epoxy resins and would Spurrs show the same problem?

- Should I reduce the staining time from 30 mins?
- Adjust the temperature from 60 degrees C for staining?
- Rinse my sections for longer with 70% ethanol after staining?

2. I have used osmium tetroxide to fix lipids in the citrus rind tissues,
with mixed results.
I have also seen some success in oil fixation by applying Sudan Black B
to the tissue prior to chemical fixation. I assume it binds and reduces
the oil solubility. I am going to test this by treating tissue with SBB
prior and post chemical fix, and comparing.

- SBB is made up in 70% ethanol. As a postfix, would it be most logical
to introduce it at the 70% step in my ethanol dehydration series? (10 to
100%)

thanks kindly, Toby Knight (struggling PhD).


--------------------------------------------------------
Toby Knight
PhD student

Department of Horticulture, Viticulture and Oenology
The University of Adelaide
Plant Research Centre
Waite Campus, PMB 1
Glen Osmond, SA 5064
AUSTRALIA

Tel: +61 8 8303 6668 or 8303 6665
HVO: +61 8 8303 7242
Fax: +61 8 8303 7116
Email: tknight-at-waite.adelaide.edu.au
--------------------------------------------------------




From daemon Wed Feb 23 07:45:46 2000



From: Dr. Manfred Rohde :      mro-at-GBF.de
Date: Wed, 23 Feb 2000 07:26:57 +0100
Subject: Immuno-labeling of glutaraldehyd, osmium embedded samples in

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Dear Microscopist,

has anyone out there any experiences with immuno-labeling of osmificated and
Spurr embedded samples. We have tried out etching, oxidizing sections with
hydrogen peroxide (10% for 15 min) or sodium methylat (1% for 15 min)
without any significant increase of label intensity (Ab used is polyclonal
from rabbit, protein A purified)

Any suggestions are welcome. Manfred



From daemon Wed Feb 23 07:45:48 2000



From: Fatima Merchant :      merchant-at-persci.com
Date: Wed, 23 Feb 2000 07:48:45 -0600
Subject: Re: pinhole grids

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Nestor,

It made it her ok. I thought I was getting pretty good
service all week. But I know what evils hide in name
servers. Not nearly to the extent you do:)

As always you efferots are welcome. You provide the
pipe for one of the best resorces on the net.

Gordon

G. C,. Couger
624 Cheyenne
Stillwater, OK 74075-1411
405 624 2855 between 3:00 pm to 1:00 am CST
405 742 2758 cell
----- Original Message -----
} From: "Nestor J. Zaluzec" {zaluzec-at-sparc5.microscopy.com}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Tuesday, February 22, 2000 7:20 PM



}
} Dear All:
}
} I was wondering if anyone out there is aware of a company
} that sells pinhole grids. I am looking for grid(2D) of
} pinholes that are approximately 15-20 microns in diameter
} with a center to center separation of about 150-200 microns.
}
} I would greatly appreciate any information that you could
} provide regarding the commercial availability of similar
} pinhole grids.
}
} TIA,
} Fatima Merchant
}
}
} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {}
}
} Fatima Merchant, Ph.D.
} Senior Research Engineer
} Perceptive Scientific Instruments, Inc.
} 2525 South Shore Blvd., Suite 100
} League City, Texas 77573
}
} Telephone: (281) 334-3027 Ext: 230
} Toll Free: (800) 288-3027 Ext: 230
} Facsimile: (281) 538-2222
} Email: merchant-at-persci.com
}
} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {}
}
}




From daemon Wed Feb 23 08:26:47 2000



From: G Peranzi :      peranzi-at-bichat.inserm.fr
Date: Wed, 23 Feb 2000 08:25:18 -0600
Subject: KNEWS ABOUT GLOW DISCHARGE ACCESSORY

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-----Original Message-----
} From: Valdes Erica R Dr. SBCCOM
Sent: Tuesday, February 22, 2000 1:26 PM
To: 'microscopy-at-sparc5.Microscopy.Com'


We got a sputter-coater system (VG Microtech SC500) that we would like to
convert
into a glow discharge. Does anybody have done it already?
We got electronic schemes to do the High Voltage unit control, but we miss the
mecanical design to fit into our vacuum chamber (of the SC500). Does anybody
know
how to do?
We know that an acessory (for the Microtech) have been developped some years
ago
(GD350A) but it is not anymore available. What are the modification made to
use this
chamber?
Jean-Jacques LACAPERE
INSERM U 410
FacultŽ de MŽdecine X. Bichat
Paris FRANCE




From daemon Wed Feb 23 08:26:48 2000



From: Jo Verbeeck :      joverbee-at-ruca.ua.ac.be
Date: Wed, 23 Feb 2000 08:25:41 -0600
Subject: TEM EELS:How to focus ESI images?

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Hello all,

I'm using a CM30 FEG with GIF PEELS atached to it. I'm getting confused
about focussing an energy filtered image.

GATAN proposes to focus the image on the TV camere at a loss of
100eV.Apparently it will be focused more or less OK at a higher loss say
741eV.
DM uses the voltage offset which changes the HT of the microscope. So as
far as I understand the focus should be OK for every energy loss(no
refocussing should be needed at all) . Although in practice it is not. The
trick with focussing at 100eV works better than not focussing at all but
it is not satisfactory for high spatial resolution.

In the Phd of M.Schenner (TU Wien "The quantification problem in EELS
Microanalysis") I found a callibration procedure to find a defocus to
correct for chromatic abberation as a function of the energy loss you are
interested in. But he seems to use the drift tube in the GIF.

The question now: How should I focus my ESI images and what is the
explanation behind it?

Thanks in advance,

Jo

*************************************************************
* Jo Verbeeck *
* University of Antwerp *
* Dept. EMAT (Electron Microscopy for Materials Research) *
* e-mail: joverbee-at-ruca.ua.ac.be *
* tel: +32(0)3 218 02 49 *
* fax: +32(0)3 218 02 57 *
*************************************************************




From daemon Wed Feb 23 10:17:11 2000



From: Greg Erdos :      gwe-at-biotech.ufl.edu
Date: Wed, 23 Feb 2000 09:37:47 -0500
Subject: Re: Immuno-labeling of glutaraldehyd, osmium embedded samples

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Material fixed like yours works only rarely. You might try etching with
saturated sodium metaperiodate for one hour followed by water washes before
incubating with the primary.
That is assuming that your antigen is not periodate sensitive.

At 07:26 AM 02/23/2000 +0100, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Gregory W. Erdos, Ph.D. Ph. 352-392-1295
Assistant Director, Biotechnology Program
PO Box 110580 Fax:
352-846-0251
University of Florida
Gainesville, FL 32611





From daemon Wed Feb 23 10:17:13 2000



From: William Tivol :      tivol-at-wadsworth.org
Date: Wed, 23 Feb 2000 10:03:08 -0500
Subject: Re: pinhole grids

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Fatima Merchant wrote:

} } I was wondering if anyone out there is aware of a company
} } that sells pinhole grids. I am looking for grid(2D) of
} } pinholes that are approximately 15-20 microns in diameter
} } with a center to center separation of about 150-200 microns.
} }
} } I would greatly appreciate any information that you could
} } provide regarding the commercial availability of similar
} } pinhole grids.
} }

Dear Fatima,
I asked about the same question and got a reply from
Janko Otto (otto-at-quantifoil.com). He wrote me:

"I read a discussion about making holey films . We had
solving the problem using semiconductor lithographic techniques.

We have been producing QUANTIFOIL, a perforated foil with pre-definable
hole size shape and arrangement. The variation among the hole sizes is
small: the standard deviation for their distribution is well below 0.1
µm within a batch; the reproducibility for the average hole size between
batches is 0.2 µm. The shapes of the holes in the foils produced so far
are circular and square. Until now, their arrangements have been
orthogonal.

Foils with any desired hole size in the micrometer range, and with any
shape and arrangement can be fabricated. Any of these three parameter
can also be varied in a defined manner within one foil.

Until now, we produce three different types of QUANTIFOIL:

Type Hole Repetition distancee
um um
R1.2/1.3 ~1.2 2.5 (circular holes)
R2/2 ~2 4 (circular holes)
S7/2 ~7 9 (square holes)

QUANTIFOIL is generally delivered as a carbon foil; it can also be
obtained strengthened with plastic film.

If you interested in these perforated foil, I can send you some samples."

He may be able to produce what you want. I have not yet tried
his grids out, but I intend to when I get my specimens ready. I am not
affiliated with Quantifoil, just a potential user. Good luck.
Yours,
Bill Tivol





From daemon Wed Feb 23 10:17:14 2000



From: William Tivol :      tivol-at-wadsworth.org
Date: Wed, 23 Feb 2000 10:38:42 -0500 (EST)
Subject: Re: Cool Prep (Water Coolers)

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*************************************************************
* Jo Verbeeck *
* University of Antwerp *
* Dept. EMAT (Electron Microscopy for Materials Research) *
* e-mail: joverbee-at-ruca.ua.ac.be *
* tel: +32(0)3 218 02 49 *
* fax: +32(0)3 218 02 57 *
*************************************************************

---------- Forwarded message ----------


} We are currently using Cool Prep in our Coolwell cooler system. This is a
} liquid cleaner & slime preventer that helps keep mineral deposits to a
} minimum in the cooling system. It also helps prevent corrosion in the
} water passages without harming hoses, gaskets or seals. Since Coolwell is
} no longer in business does anyone know of a replacement product for Cool
} Prep.
}
} Can we simply switch to ethylene glycol?

Dear Robert,
The suggestion of using distilled water and letting the system
come to equilibrium might work in a truly closed system composed of only
one material, but if you need to add water, or if you have several metals
in the system--as we do--this will be unsatisfactory in the long run.
We have found that Aqua-Treet 42 works very well in our Haskris system.
This product is made by Aqua Laboratories, Inc., PO Box 645, 8 Indus-
trial Way, Amesbury MA, (978) 388-3989. I have no relationship to this
company except as a satisfied user of their product.
Yours,
Bill Tivol



From daemon Wed Feb 23 18:10:14 2000



From: Walck. Scott D. :      walck-at-ppg.com
Date: Wed, 23 Feb 2000 11:42:55 -0500
Subject: DTSA and EMMFF 1.1?

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I am running two copies of DTSA, one is an older version and one is the
newer one that will run on a power Mac. I am also fairly new to DTSA. The
old version does not have the MSA format capability for reading and writing
files, but the newer one does. However, the new one will read two versions
of MSA format, 1.0 and 1.1 but will only save in 1.1. Now I'm confused. I
asked Nestor where I could get the latest MSA description of the MSA format
and he sent me what I already have, EMMFF.total from of the EMMPDL/MAS-Lib
files. I know that Nestor was on that committee. So what gives with MSA
version 1.1 in the latest version of DTSA? Why don't they have the 1.0
option? The keywords are not even the same.



-Confused in Pittsburgh.


Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)




From daemon Wed Feb 23 18:10:16 2000



From: wft03-at-health.state.ny.us
Date: Wed, 23 Feb 2000 12:13:50 -0500
Subject: Re: Cool Prep (Water Coolers)

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Why not use a liquid that has NO water in it. That solved my problem in
vibratory polishing of dissimilar metals in an alumina slurry. Ethylene
glycol or propylene glycol, (less toxic), are possibilities.


Dear Bernie,

That will work unless there is some microorganism which will

grow in ethylene glycol. One could also just buy commercial anti-freeze

and get some additional corrosion protection. I don't think that there

would be any problem with the circulation pump, since the viscosity

of glycol is similar to that of water. Keeping the fluid in the system for

some decades has different requirements from using it over a period

of hours, though.

Yours,

Bill Tivol




From daemon Wed Feb 23 18:10:16 2000



From: Carl Henderson :      chender-at-umich.edu
Date: Wed, 23 Feb 2000 12:22:05 -0500
Subject: Re: DTSA and EMMFF 1.1?

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Look for a "Write MSA 1.0" plug-in in the "extras Folder" of your
DTSA download and move it to the "Plug-ins" folder at the same level
as DTSA.



At 11:42 AM -0500 2/23/00, Walck. Scott D. wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

======================================
Carl Henderson
Electron Microbeam Analysis Laboratory
University of Michigan
2501 C.C. Little Bldg.
Ann Arbor, MI 48109-1063 USA
(734) 936-1550 FAX (734) 763-4690
======================================



From daemon Wed Feb 23 18:10:16 2000



From: Tobias Baskin :      BaskinT-at-missouri.edu
Date: Wed, 23 Feb 2000 11:39:28 -0600
Subject: (FE)SEM, sputter coating puzzle

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Greetings,
I wonder if anyone could explain the following observation
to me. I have been sputter coating with platinum and viewing the
sample with a FESEM (Hitatchi 4700, below lens type). In a certain
type of sample, the image appears darker than the background where
there is sample. Now these samples are perhaps a bit unusual. They
are very thin, starting off life as 1.75 um methacrylate sections of
a plant root adhered to a glass coverslip, followed by extraction in
hot acidic peroxide to remove all organic material except crystalline
cellulose. The cellulosic cell walls left behind on the coverslip are
nominally 100 nm thick and perhaps flattened further by the
processing. The cell walls are present in the sample as either cross
sections or as small regions laying in the plane of the coverslip.
These regions can be anywhere from the size of a cell (10 x 40 or so
microns) down to small portions thereof. All of this remaining cell
wall is darker than the surrounding background. I am putting on a
thin coat of Pt, somewhere around 2 nm, and imaging at 2 kV (I have
seen the same thing at 5 kV). It really puzzles me that my sample is
darker than background. Any explanations???

THanks,
Tobias
_ ____ ^ __ ____ Tobias I. Baskin
/ \ / / \ / \ \ University of Missouri
/ | / / \ \ \ Biological Sciences
/___/ /__ /___ \ \ \__ 109 Tucker Hall
/ / / \ \ \ Columbia, MO 65211-7400 USA
/ / / \ \ \ voice: 573-882-0173
/ /____ / \ \__/ \____ fax: 573-882-0123



From daemon Wed Feb 23 18:10:18 2000



From: Debby Sherman :      sherman-at-btny.purdue.edu
Date: 23 Feb 2000 13:17:19 -0500
Subject: Food Science Prep.

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Hi,
Well I guess we never run out of new challenges when it comes to specimen prep. This one concerns a product that is made of a cereal bound together with a sugar syrup. The investigators want to see how the syrup behaves as to structural integrety over a period of time with and without the addition of various stabilizing components. Information would be collected at the light microscopic level.
We first thought of cryosectioning followed by examination using LM. However, the product is filled with air pockets and literally disintegrates when microtomed. It is easily cut into pieces at temperatures above freezing as long as the pieces are no thinner than3-4 mmAccempts to infiltrate it with OCT compoound prior to freezing were not satisfactory.
New thought is to use a low temperature resin to infiltrate the sample followed by UV or chemical polymerization. butThe resulting blocks could then be microtomed at room temperature and imaged. It would be desirable to cut sections of 4-8 µm in thickness which is too thick for an ultramicrotome with glass or diamond knives. Also UV polymerization would probably limit us to very small sample size which may be a problem.
Any suggestions would be appreciated as to resins to use which have low viscosity, could be used to infiltrate pieces of ~0.5-1cm sq, polymerize at low temperatures (+4 to 0oC range) using UV or chemical polymerization, and are preferably soft enough to section with a metal microtome blade. Any other suggestions of preparations techniques to try would be very appreciated.

Thanks in advance for your input.
Debby

Debby Sherman, Manager Phone: 765-494-6666
Microscopy Center in Agriculture FAX: 765-494-5896
Dept. of Botany & Plant Pathology E-mail: sherman-at-btny.purdue.edu
Purdue University
1057 Whistler Building
West Lafayette, IN 47907-1057



From daemon Wed Feb 23 18:10:18 2000



From: Hank Adams :      hpadams-at-bcm.tmc.edu
Date: Wed, 23 Feb 2000 11:14:42 -0600
Subject: TEMs

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Dear listservers, I need to determine the value of a Philips 410 that has
been on service contract until it was decommissioned last year. I need this
information for an insurance claim. Is there a company or dealer of used em
equipment that can help me?
Thanks
Hank Adams
Integrated Microscopy Core
Molecular and Cellular Biology
Baylor College of Medicine
Houston, Tx



From daemon Wed Feb 23 18:10:19 2000



From: Timothy Schneider :      Timothy.Schneider-at-mail.tju.edu
Date: Wed, 23 Feb 2000 14:29:55 -0500
Subject: GLOW DISCHARGE

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Sorry if this is a second post, but we just got an e-mail saying the
first was rejected as spam, so here goes:






Hi:

I want to be able to "Glow Discharge" carbon coated copper grids for the
purpose of making them hydrophilic and negatively charged, so that I can
spread aqueous suspensions on them. I have a Denton Vacuum Desk ll sputter
coater with a "etch" mode. The question I have for my fellow list servers
is: will "etching" the grids do the same thing as "Glow Discharging" them?
Many Thanks, Tim


Timothy Schneider, Director of Electron Microscopy
Department of Pathology
Thomas Jefferson University
Room 229 Jeff Hall
1020 Locust St.
Philadelphia, Pa.
19107
215-503-4798
Timothy.Schneider-at-Mail.TJU.EDU



From daemon Wed Feb 23 18:10:21 2000



From: jillp-at-telecomedia.co.jp
Date: Wed, 23 Feb 2000 04:43:29 -0600
Subject: Version 5.0 is Now Available

Contents Retrieved from Microscopy Listserver Archives
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From daemon Wed Feb 23 18:10:21 2000



From: jillp-at-telecomedia.co.jp
Date: Wed, 23 Feb 2000 04:43:29 -0600
Subject: Version 5.0 is Now Available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
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To Unsubscribe your email address from our Opt-In listserver, see end of
message.

Dear Subscriber,

Introducing one the HOTTEST selling softwares of the year.

"NMS SOFTWARE" The Software no computer should be without!

Its Simple to Use and Easy to Understand.

Now you can quickly and easily have access to the most powerful internet investigation sites and databases available today!

"NMS v5.0" now with the latest IP Phone.

Make calls anywhere in the world for free.

Also with Unclaimed Money Locator, find out if
you are owed money or property in your state.

*******************************************************

The "N M S SOFTWARE" is absolutely astounding!

Every computer should have this!

Here's what you can obtain:
---------------------------
Files that the government has on you.

You know who "The F*I and.......

License plate number!

Get anyone's name and address with just a license plate number!

(Find that girl you met in traffic!)

Driving record!

Get anyone's driving record

Social security number!

Trace anyone by social security number!

Free Internet Access

Address!

Get anyone's address with just a name!

Unlisted phone numbers!

Get anyone's phone number with just a name - even unlisted numbers!

Locate!

Long lost friends, relatives, a past lover who broke your heart!

Send anonymous e-mail completely untraceable!

Investigate anyone!

Use the sources that private investigators use (all on the Internet)
secretly!

Ex-spouse!

Learn how to get information on an ex-spouse that will help you

win in court! (Dig up old skeletons)

Criminal search-background check!

Find out about your daughters boyfriend!

(or her husband)

Find out!

If you are being investigated!

Neighbors!

Learn all about your mysterious neighbors! Find out what they

have to hide!

People you work with!

Be astonished by what you'll learn about people you work with!

Education verification!

Did he really graduate college? Find out!
**********************************************************
Just insert the floppy disk and Go!
**********************************************************
Data Bases Cover the US and Canada.

You will be shocked and amazed by the secrets that can be

discovered about absolutely everyone! Find out the secrets

they don't want you to know! About others, about yourself!
--------------------------------------
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(ORDERS OUTSIDE THE USA, ADD $25.00)

DON'T WAIT TO GET STARTED... here's what to do:

STEP 1 - Compose a new message using the order form text below

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-----------------------------------------
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system by calling 708-562-1778
------------------------------------------
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} } } Mail-in Order Form { { {

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Address:

City:

State:

Zip:

E-mail address:

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[ ] Visa [ ] MasterCard [ ] Check

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From daemon Wed Feb 23 18:10:22 2000



From: Doug Cromey :      doug-cromey-at-ns.arizona.edu
Date: Wed, 23 Feb 2000 13:10:48 -0700
Subject: Can EELS, WDS or FT-IR localize As in Biol. tissues?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Colleagues,

We are interested in localizing arsenic in biological tissues (toxicology
research), but have been unable to come up with a technique that will give
us the cellular localization information we're looking for (sub-cellular
would be even better).

We have tried TEM with energy dispersive x-ray microanalysis and our
concentrations are apparently too low, so we are unable to detect arsenic.

A few years ago we looked into using the autometallographic techniques that
have been published by Dr. Gorm Dansher & his colleagues. We were told
that the photographic emulsions required were no longer available.

In a brainstorming session yesterday several techniques were bantered
about, but in truth we are not familiar enough with the techniques to know
if they would suit our needs. Does EELS have anything to offer, or perhaps
wavelength dispersive spectoscopy (WDS)? How about some of the new FT-IR
instruments? Are there any other techniques that might be able to localize
arsenic and/or indicate its state of oxidation?

Vendors are welcome to reply.

Yours,
Doug Cromey
....................................................................
: Douglas W. Cromey, M.S. Dept. of Cell Biology & Anatomy :
: Research Specialist, Principal University of Arizona :
: (office: AHSC 4212A) P.O. Box 245044 :
: (voice: 520-626-2824) Tucson, AZ 85724-5044 USA :
: (FAX: 520-626-2097) (email: doug-cromey-at-ns.arizona.edu):
:...................................................................:
http://www.pharmacy.arizona.edu/exp_path.html
Home of: "Microscopy and Imaging Resources on the WWW"



From daemon Wed Feb 23 18:10:23 2000



From: Scott Wight :      scott.wight-at-nist.gov
Date: Wed, 23 Feb 2000 15:28:52 -0500
Subject: Re: DTSA and EMMFF 1.1?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Scott & listers

Go to http://www.cstl.nist.gov/div837/837.02/dtsa.html and click on
"supporting files" page then download "preferences and plugins" you will
find 2 versions of the MSA write 1.0 (one is Kevex specific). It would
make life much easier on us microscopists if all manufacturers adopted the
MSA format.
Good luck,
Scott


} I am running two copies of DTSA, one is an older version and one is the
} newer one that will run on a power Mac. I am also fairly new to DTSA. The
} old version does not have the MSA format capability for reading and writing
} files, but the newer one does. However, the new one will read two versions
} of MSA format, 1.0 and 1.1 but will only save in 1.1. Now I'm confused. I
} asked Nestor where I could get the latest MSA description of the MSA format
} and he sent me what I already have, EMMFF.total from of the EMMPDL/MAS-Lib
} files. I know that Nestor was on that committee. So what gives with MSA
} version 1.1 in the latest version of DTSA? Why don't they have the 1.0
} option? The keywords are not even the same.
}
} -Confused in Pittsburgh.
}
} Scott D. Walck, Ph.D.
} PPG Industries, Inc.
} Glass Technology Center
} Guys Run Rd. (packages)
} P. O. Box 11472 (letters)
} Pittsburgh, PA 15238-0472
} Walck-at-PPG.com
} (412) 820-8651 (office)
} (412) 820-8161 (fax)

For the weather watchers: Mostly sunny, 60 F, snow is melted away.

-------------------note: new mailing address----------------------
Scott Wight e-mail: scott.wight-at-nist.gov
NIST 222/A113 W voice: 301-975-3949
100 Bureau Dr STOP 8371 | fax: 301-417-1321
Gaithersburg, MD 20899-8371 \|/ disclaimer: Any opinion expressed
is my own and does not represent those of my employer.




From daemon Wed Feb 23 18:10:23 2000



From: Supanee Danviriyakul :      supaneed-at-foodsci.umass.edu
Date: Wed, 23 Feb 2000 15:40:10 -0500 (EST)
Subject: Image analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear all,
I am doing the image analysis of SEM micrographs. The
micrographs contain both dense (light) and air cell (dark) areas. But two
areas are not discrete. I'm having a difficulty of quantifying those
separated fractions. I wonder if anyone has any ideas of how to
solve the problem.
Looking forward for your suggestions.
Regards,
Supanee



From daemon Wed Feb 23 18:10:25 2000



From: Rejane Magalh‹es Pimentel
Date: Wed, 23 Feb 2000 19:25:00 -0300
Subject: ellipse at Image Tool

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello all,
I need to know how to draw an ellipse to measure at Image Tool software.
Thanks a lot.
Rejane Pimentel
Rejane Magalh‹es Pimentel Galindo
Functional Plant Morphology
Universidade Federal Rural de Pernambuco
ggalindo-at-elogica.com.br
Rua Maria Carolina, 417/804
51020-220 Boa Viagem



From daemon Wed Feb 23 18:10:26 2000



From: Sandra Perkins :      skperkin-at-vt.edu
Date: Wed, 23 Feb 2000 17:06:32 -0500
Subject: epoxy embedding of rat eye

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I thought I'd try this again in case it got lost in space:

Hi-

We are going to be processing rat eyes (from perfused animals) for
embedding in epoxy and I was wondering if anyone has any suggestions for
processing. We are interested in the retina, optic nerve, cornea and uveal
tract.

Thank you very much,
Sandy Perkins




From daemon Wed Feb 23 18:10:27 2000



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Wed, 23 Feb 2000 16:03:17 -0600
Subject: Re: Image analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ahh! That is always the rub, trying to get the areas of interest to stand
out as a distinct range of gray scales.

If it is just a question of noise pixels in what is otherwise a distinct
region, then you might try filtering (smoothing) your image before
thresholding. Or you might try changing your image acquisition protocol to
get less noisy images.

You might try a different signal to find something that is less subject to
overlaps. Usually I resort to backscattered electron imaging since the
dependence on atomic number of that signal typically results in distinct
gray levels.

If that won't do it, you might be able to perform some sort of image
processing to separate the regions. There are techniques available, but
there seems to be as much art as science to them. I would refer you to John
Russ' "Image Processing Handbook" or a similar text, because there is not a
short answer to that question.

Warren Straszheim

At 03:40 PM 2/23/2000 -0500, you wrote:
} Dear all,
} I am doing the image analysis of SEM micrographs. The
} micrographs contain both dense (light) and air cell (dark) areas. But two
} areas are not discrete. I'm having a difficulty of quantifying those
} separated fractions. I wonder if anyone has any ideas of how to
} solve the problem.
} Looking forward for your suggestions.
} Regards,
} Supanee



From daemon Wed Feb 23 19:00:26 2000



From: Gordon Couger :      gcouger-at-rfdata.net
Date: Wed, 23 Feb 2000 18:37:38 -0600
Subject: Re: Cool Prep (Water Coolers)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} From: {"wft03-at-health.state.ny.us"-at-sparc5.microscopy.com}
To} Why not use a liquid that has NO water in it. That solved my problem
in
} vibratory polishing of dissimilar metals in an alumina slurry. Ethylene
} glycol or propylene glycol, (less toxic), are possibilities.
}
}
} Dear Bernie,
}
} That will work unless there is some microorganism which will
}
} grow in ethylene glycol. One could also just buy commercial anti-freeze
}
} and get some additional corrosion protection. I don't think that there
}
} would be any problem with the circulation pump, since the viscosity
}
} of glycol is similar to that of water. Keeping the fluid in the system
for
}
} some decades has different requirements from using it over a period
}
} of hours, though.

Glycols won't have near the heat capacity of water. I doubt
you will find a bug that will live in ethylene glycol but you can
probably find many that can live in propylene glycol.

Any cooling system needs to have the coolant changed
periodically. Following the manufactures recommendation
is the safe way to go.

Gordon

Gordon Couger gcouger-at-couger.com

Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00




From daemon Wed Feb 23 19:00:27 2000



From: Ladd Resaerch :      ladres-at-worldnet.att.net
Date: Wed, 23 Feb 2000 18:17:38 -0600
Subject: Re: pinhole grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Dear Fatima Merchant,

This is something that we can probably help you with. We do similar jobs
for several other companies.
If you could fax a drawing with the material, OD, Thickness, number of
holes, etc. to 1-802-878-8074 we can get you a quote.

Thanks,

John Arnott
Chairman
--

LADD RESEARCH
131 Dorset Lane
Williston, VT 05495

TEL 1-800-451-3406 (US) or 1-802-878-6711 (anywhere)
FAX 1-802-878-8074
e-mail ladres-at-worldnet.att.net
web site http://www.ladd.cc


--

LADD RESEARCH
131 Dorset Lane
Williston, VT 05495

TEL 1-800-451-3406 (US) or 1-802-878-6711 (anywhere)
FAX 1-802-878-8074
e-mail ladres-at-worldnet.att.net
web site http://www.ladd.cc




From daemon Wed Feb 23 19:10:27 2000



From: Fang Qian :      fangq-at-tr.comm.mot.com
Date: Wed, 23 Feb 2000 18:57:07 -0600
Subject: Cryotransfer system

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi, There,

We are considering to purchase a cryotransfer system for our 2010F TEM.
There are two major suppliers: Gatan and Oxford. We are not sure which
one works better since this is the first time dealing with this
technique.

Could you please send me your comments and experiences on these two
vendors? Our samples are mostly soft tissues: Polymers, surfactants,
cell membranes, and proteins. Your help is highly appreciated.

Maoxu Qian
****************************
* Maoxu Qian, Ph.D. *
* Dept of MSE, box 352120 *
* University of Washington *
* Seattle, WA 98195 *
* mxq-at-u.washington.edu *
* (206)616-3973(phone) *
* (206)543-3100(fax) *
****************************



From daemon Wed Feb 23 19:50:19 2000



From: David Henriks :      Henriks-at-CompuServe.COM
Date: Wed, 23 Feb 2000 19:31:13 -0600
Subject: Tripod Polisher Workshop

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Workshop Objective

This course will cover all aspects of pre-thinning and focus on final
thinning via Tripod Polishing. Due to the limited class size and the
extensive hands-on opportunities, this course is well suited to novices as
well as advanced Tripodders. Attendees will also learn the latest
techniques available in ion milling, plasma cleaning and ion beam sputter
deposition and etching for TEM and SEM samples.

The workshop will be held in San Clemente, CA on May 26-27, 2000. Please
call for more information or visit our web site ( www.southbaytech.com )
for registration information.


***************************************************************************

David Henriks
Vice President TEL: 800-728-2233
(toll free in the USA)
South Bay Technology, Inc. +1-949-492-2600
1120 Via Callejon FAX: +1-949-492-1499
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

***************************************************************************
} } } } } Please visit us at http://www.southbaytech.com { { { { {

Manufacturers of precision sample preparation equipment and supplies for
metallography, crystallography and electron microscopy.




From daemon Wed Feb 23 20:10:23 2000



From: Maoxu Qian :      mxq-at-u.washington.edu
Date: Wed, 23 Feb 2000 20:04:56 -0600
Subject: Cryo holder TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



}
}
} Hi, There,
}
} We are considering to purchase a cryotransfer system for our 2010F TEM.
} There are two major suppliers: Gatan and Oxford. We are not sure which one
} works better since this is the first time dealing with this technique.
} Could you please send me your comments and experiences on these two
} vendors? Our samples are mostly soft tissues: Polymers, surfactants, cell
} membranes, and proteins. Your help is highly appreciated.
}
} Maoxu Qian
} ****************************
} * Maoxu Qian, Ph.D. *
} * Dept of MSE, box 352120 *
} * University of Washington *
} * Seattle, WA 98195 *
} * mxq-at-u.washington.edu *
} * (206)616-3973(phone) *
} * (206)543-3100(fax) *
} ****************************
}
}
}
}
}




From daemon Thu Feb 24 08:25:15 2000



From: Divakar R :      divakar-at-igcar.ernet.in
Date: Thu, 24 Feb 2000 11:41:30 +0530
Subject: Re: Cool Prep (Water Coolers)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


A related question from India. We have been using DM (demineralised) water in our JEOL 2000 EX II cooled by a Neslab chiller for the last ten years. This requires change every 6 months approximately. What additives can be used to prevent algae (? it is green and slimy and I am not a biologist) growth? Something obtainable locally (chemical name rather than trade names) will be most useful to me. Thanks very much.

-----Original Message-----
} From: William Tivol [SMTP:tivol-at-wadsworth.org]
Sent: Thursday, February 24, 2000 10:25 AM
To: Robert Champaign
Cc: microscopy-at-sparc5.microscopy.com


} We are currently using Cool Prep in our Coolwell cooler system. This is a
} liquid cleaner & slime preventer that helps keep mineral deposits to a
} minimum in the cooling system. It also helps prevent corrosion in the
} water passages without harming hoses, gaskets or seals. Since Coolwell is
} no longer in business does anyone know of a replacement product for Cool
} Prep.
}
} Can we simply switch to ethylene glycol?

Dear Robert,
The suggestion of using distilled water and letting the system
come to equilibrium might work in a truly closed system composed of only
one material, but if you need to add water, or if you have several metals
in the system--as we do--this will be unsatisfactory in the long run.
We have found that Aqua-Treet 42 works very well in our Haskris system.
This product is made by Aqua Laboratories, Inc., PO Box 645, 8 Indus-
trial Way, Amesbury MA, (978) 388-3989. I have no relationship to this
company except as a satisfied user of their product.
Yours,
Bill Tivol





From daemon Thu Feb 24 08:25:20 2000



From: Anita Garg :      Anita.Garg-at-lerc.nasa.gov
Date: Thu, 24 Feb 2000 07:55:24 -0500
Subject: Re: Allied Multiprep Polisher for TEM samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Colleagues
Does anybody have experience on the Allied MultiPrep Polishing
System, especially in terms of getting a good TEM sample? How well do
the Allied's Precision Cross-Section Tool and TEM Wedge Polisher work
on this semi automatic multiprep polisher?
Also, how does their TEM Wedge Polisher compare with the South Bay
Tripod Polisher?
TIA
Anita
*******************************************
Dr. Anita Garg
NASA Glenn Research Center at Lewis Field
Advanced Metallics Branch
Mail Stop 49-3
21000 Brookpark Road
Cleveland, OH 44135
Phone : (216) 433-8908
Fax : (216) 977-7132
E-mail : Anita.Garg-at-grc.nasa.gov
*******************************************



From daemon Thu Feb 24 08:42:01 2000



From: Dan May :      dmay-at-iti-med.com
Date: Thu, 24 Feb 2000 08:26:39 -0600
Subject: PINHOLE GRIDS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Fatima Merchant,
I used to work for a company called Buckbee Mears. They have been making
tight tolerance electroformed Ni pinholes for many, many years. They are
located in St. Paul, MN and their phone number is 651-228-6400. Good luck.
Dan May




From daemon Thu Feb 24 08:42:02 2000



From: Leona Cohen-Gould :      lcgould-at-mail.med.cornell.edu
Date: Thu, 24 Feb 2000 10:47:41 -0400
Subject: Re: epoxy embedding of rat eye

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} Hi-
}
} We are going to be processing rat eyes (from perfused animals) for
} embedding in epoxy and I was wondering if anyone has any suggestions for
} processing. We are interested in the retina, optic nerve, cornea and uveal
} tract.
}
} Thank you very much,
} Sandy Perkins

****************************
Hi Sandra,

Eyes are a real pain. There are so many layers of different cell types,
and each seems to infiltrate differently. Having worked on a fir number of
eyes, I woauld make the following suggestions:
Process the cornea separately. It is almost pure collagen. It will not
osmicate all that noticeably, so don't be surprised when it doesn't turn
dark brown or black. Give it long infiltration times, in vaccuum if
possible.

If you don't need the optic nerve attached to the eye, dissect it free and
process it. Give it slighlty longer than usual dehydration and
infiltration steps to be sure you'e got the myelin taken care of.

As for the rest: remove the lens and the vitreous so that you have good
acess to the retina. Be careful not to disrupt the retina when you remove
the vitreous. Once the lens is out, you can usually get the vitreous out
by gently blotting against a Kimwipe. You may want to split the orb into
hemispheres. Just be sure not to take any sections from the cut edge,
since you will disturb the structure there when you cut. Again, you'll
nees longer dehyration and infiltration times, since the sclera is really
tough. I use 30 minutes in each step of graded acetone, propylene oc=xide
then a graded infiltraion with PO:resin (Epon analog) 2:1 for 30 min, 1:1
overnight, 1:2 2 hours, pure resin overnight, on a rotator.

I'm sure there ore others out there, with their own "pet protocol" for
eyes. Its all a bit of magic and luck, isn't it?

Good luck,
Lee

Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Confocal Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175




From daemon Thu Feb 24 08:42:03 2000



From: ferrari-at-comp.uark.edu (Michael Ferrari)
Date: Thu, 24 Feb 2000 08:30:12 -0800
Subject: WI objectives for BH-2 RFCA

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All,

Not sure this went out earlier...I've inherited an Olympus BH-2 RFCA
upright that I'd like to equip with WI lenses for live cell fluorescence
work. This is a 160mm tube length scope, and Olympus is now only
manufacturing the 60X WI fluor objective (I think/hope). Any leads to
sources of compatible WI objectives in the 10-60X range much appreciated.
Please reply offline. Thanks.

Mike

Michael Ferrari
Department of Biological Sciences
629 Science Engineering (SCEN)
University of Arkansas
Fayetteville, Arkansas 72701
Ph: 501-575-6372
Fax: 501-575-4010




From daemon Thu Feb 24 17:33:53 2000



From: amy-at-www.molbio.Vanderbilt.Edu (amy-at-helix.molbio.vanderbilt.edu)
Date: Thu, 24 Feb 2000 10:14:40 -0600
Subject: image processing - microdensitometer available

Contents Retrieved from Microscopy Listserver Archives
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We are interested in selling our Perkin-Elmer Model
1010M microdensitometer at a very reasonable price. It
was purchased as a reconditioned model from Perkin-Elmer
eight years ago and is in very good condition. Technical
manual is included.

If interested, please contact:

Amy Kendall
Department of Molecular Biology
Vanderbilt University
Box 1820, Station B
Nashville TN 37232
(615) 322-2012
amy-at-helix.molbio.vanderbilt.edu



From daemon Thu Feb 24 17:33:56 2000



From: Russell E. Cook :      recook-at-anl.gov
Date: Thu, 24 Feb 2000 11:03:00 -0600
Subject: RE: Cool Prep (Water Coolers)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


R. Divakar wrote, "A related question from India. We have been using DM
(demineralised) water in our JEOL 2000 EX II cooled by a Neslab chiller for
the last ten years. This requires change every 6 months approximately. What
additives can be used to prevent algae (? it is green and slimy and I am
not a biologist) growth? Something obtainable locally (chemical name rather
than trade names) will be most useful to me."

I quote here recommendations from the Haskris Co. (we have several Haskris
chillers):

1. If algae is present, flush the system with one of two alternatives: add
1 cup of chlorine bleach or one pint of hydrogen peroxide per 15 gallons of
water. Circulate 20-30 minutes. Drain the system and refill with clean
water.

2. To prevent regrowth of algae, one of the following additives are
recommended:
a. 10% solution of lab grade (no additives) ethylene glycol.
b. Dichlorophene. This is an insoluble white powder which is a
fungicide/bacteriacide. It should be sprinkled evenly across the entire
surface of the water in the chiller's tank.
c. 8 parts per million chlorine.

----------------------------------------------------------------------
Russell E. Cook, Ph.D.
Electron Microscopy Center for Materials Research
Argonne National Laboratory
Materials Science Division, Building 212
9700 South Cass Avenue
Argonne, IL 60439-4838
(630)252-7194
FAX: (630)252-4289
recook-at-anl.gov




From daemon Thu Feb 24 17:33:56 2000



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Thu, 24 Feb 2000 09:02:46 -0800
Subject: Re: (FE)SEM, sputter coating puzzle

Contents Retrieved from Microscopy Listserver Archives
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Dear Tobias,
The most logical explanation for this phenomenon is that the carbon-based
cell material is of a lower average atomic number, even with the thin Pt
coating, than the glass coverslip (SiO2) it is located on, also with the Pt
coat. The secondary electron yield is directly proportional to the average
atomic number, if topographic effects are not in evidence, which they won't
be on such a thin sample.
At 11:39 AM 2/23/00 -0600, you wrote:
} Greetings,
} I wonder if anyone could explain the following observation
} to me. I have been sputter coating with platinum and viewing the
} sample with a FESEM (Hitatchi 4700, below lens type). In a certain
} type of sample, the image appears darker than the background where
} there is sample. Now these samples are perhaps a bit unusual. They
} are very thin, starting off life as 1.75 um methacrylate sections of
} a plant root adhered to a glass coverslip, followed by extraction in
} hot acidic peroxide to remove all organic material except crystalline
} cellulose. The cellulosic cell walls left behind on the coverslip are
} nominally 100 nm thick and perhaps flattened further by the
} processing. The cell walls are present in the sample as either cross
} sections or as small regions laying in the plane of the coverslip.
} These regions can be anywhere from the size of a cell (10 x 40 or so
} microns) down to small portions thereof. All of this remaining cell
} wall is darker than the surrounding background. I am putting on a
} thin coat of Pt, somewhere around 2 nm, and imaging at 2 kV (I have
} seen the same thing at 5 kV). It really puzzles me that my sample is
} darker than background. Any explanations???
}
} THanks,
} Tobias

Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Thu Feb 24 17:34:01 2000



From: HILDEGARD CROWLEY :      hcrowley-at-du.edu
Date: Thu, 24 Feb 2000 10:43:06 -0700 (MST)
Subject: Re: Sudan Black staining and fixing

Contents Retrieved from Microscopy Listserver Archives
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Dear Toby,

You have a basic problem - free lipids interfere with the polymerization
of epoxies. Since you are having mixed results with osmium not all lipids
may be fixing adequately. If possible, try keeping the specimens in
osmium overnight, on a rotator. (After the first hour of exposure to
osmium, change to fresh osmium). I have done this very successfully with
lipid rich structures. Hope it works.

Hildy Crowley
University of Denver
Denver, CO



From daemon Thu Feb 24 17:34:01 2000



From: HILDEGARD CROWLEY :      hcrowley-at-du.edu
Date: Thu, 24 Feb 2000 10:53:58 -0700 (MST)
Subject: Re: Immuno-labeling of glutaraldehyd,

Contents Retrieved from Microscopy Listserver Archives
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On Wed, 23 Feb 2000, Dr. Manfred Rohde wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear Microscopist,
}
} has anyone out there any experiences with immuno-labeling of osmificated and
} Spurr embedded samples. We have tried out etching, oxidizing sections with
} hydrogen peroxide (10% for 15 min) or sodium methylat (1% for 15 min)
} without any significant increase of label intensity (Ab used is polyclonal
} from rabbit, protein A purified)
}
} Any suggestions are welcome. Manfred
}
}
}
Hello,

You may have a problem which you do not recognize. Spurr's really has no
place in immunocytochemistry. It is highly crosslinked. As you might
know, epoxies not only crosslink, they also chemically combine with tissue
proteins making them unavailable. Also, a highly stable, highly
crosslinked embeddding medium will cut a shiny, very smooth surface on
sections. The surface relief of such a section is extremely low, and, of
course, the more surface relief, the more chance for the antigen to be
exposed to markers. You might also have a paucity of antigen which
compounds all problems.

If you want to stay with epoxies, I would suggest switching to a soft
Epon-Araldite for starters. If that does not work, then my suggestion is
to go to LR Gold. A block of LR Gold does not "cut", but cleaves, causes
something like a triple increase in surface relief thus giving more
antigens the chance to be exposed.

If I am not making myself clear, please e-mail me. I have just gone
through 2yrs of frustration with stuff like this, but this week the paper
is going out!!!!

Bye,
Hildy Crowley
University of Denver
Denver, CO

P.S. I am not making all this up. I can reference everything, but do not
have the time to do that in this format



From daemon Thu Feb 24 17:34:04 2000



From: David Hoyle :      dhoyle-at-istar.ca
Date: Thu, 24 Feb 2000 13:22:52 -0800
Subject: Cooling water/glycol Danger

Contents Retrieved from Microscopy Listserver Archives
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Before adding glycol to your Haskris water chiller check with the
manufacture of the microscope. If you use glycol to cool a Hitachi
microscope you will damage the plastic water lines and they will all need
to be replaced after a few years. I have seen this happen a few times now
after people have used this advice from the list server. Please check with
your service engineer first.

To those users of Hitachi S2500/S570, glycol is used to cool the objective
lense but only on these 2 instruments.

I am a service engineer for Hitachi in Canada and the above opinion is my
opinion and not that of Hitachi.


David Hoyle
Nissei Sangyo Canada
http://www.nsctoronto.com/
service-at-nsctoroto.com (general mail)
dhoyle-at-istar.com (direct mail)



From daemon Thu Feb 24 17:34:04 2000



From: Heather A Owen :      owenha-at-csd.uwm.edu
Date: Thu, 24 Feb 2000 12:24:27 -0600 (CST)
Subject: RE: Cool Prep (Water Coolers)

Contents Retrieved from Microscopy Listserver Archives
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On Thu, 24 Feb 2000, Russell E. Cook wrote:

} I quote here recommendations from the Haskris Co. (we have several Haskris
} chillers):
}
} 1. If algae is present, flush the system with one of two alternatives: add
} 1 cup of chlorine bleach or one pint of hydrogen peroxide per 15 gallons of
} water. Circulate 20-30 minutes. Drain the system and refill with clean
} water.
}
} 2. To prevent regrowth of algae, one of the following additives are
} recommended:
} a. 10% solution of lab grade (no additives) ethylene glycol.
} b. Dichlorophene. This is an insoluble white powder which is a
} fungicide/bacteriacide. It should be sprinkled evenly across the entire
} surface of the water in the chiller's tank.
} c. 8 parts per million chlorine.
}
} ----------------------------------------------------------------------
} Russell E. Cook, Ph.D.
} Electron Microscopy Center for Materials Research
} Argonne National Laboratory
} Materials Science Division, Building 212
} 9700 South Cass Avenue
} Argonne, IL 60439-4838
} (630)252-7194
} FAX: (630)252-4289
} recook-at-anl.gov
}
}


I've been using dichlorophene in our Haskris chillers following the
incident related below. If you want to buy some, dichlorophene is listed
in several chemical company catalogs as:

2,2'-Methylenebis(4-chlorophenol)

Considering how I was planning to use it, I bought technical grade (90%),
since it was substantially less expensive than higher grades and have not
experienced any problems.

A complete replacement of our chiller water (along with the water in the
scope and lines) was required about a year ago when the water pump in our
TEM Haskris had to be replaced. Our sevice engineer flushed the system
using the concentrated hydrogen peroxide as mentioned above, and a truly
amazing amount of material spewed forth, so much so that small connectors
to the power supply clogged up a few weeks down the line, causing our HV
to shut down. Cleaning out the connectors and flushing the system again
with distilled water took care of the problem.

At the time, we were cautioned (by Haskris, I think) to use distilled
water and not deionized water in the chillers. Does anyone know why?

Heather Owen

Heather A. Owen, Director
Electron Microscope Laboratory
Department of Biological Sciences
University of Wisconsin - Milwaukee
(414)229-6816




From daemon Thu Feb 24 17:34:06 2000



From: Carl Henderson :      chender-at-umich.edu
Date: Thu, 24 Feb 2000 13:49:37 -0500
Subject: Re: Cooling water/glycol Danger

Contents Retrieved from Microscopy Listserver Archives
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OK, now I am concerned.

What is the real answer? When we had our Hitachi S-3200N installed
two years ago, the service engineer used an ethylene glycol/water
solution in the Haskris chiller. And it was pretty strong, too.
Something like 1:3 glycol:water.

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

======================================
Carl Henderson
Electron Microbeam Analysis Laboratory
University of Michigan
2501 C.C. Little Bldg.
Ann Arbor, MI 48109-1063 USA
(734) 936-1550 FAX (734) 763-4690
======================================



From daemon Thu Feb 24 17:34:08 2000



From: Christensen, Kim :      ChristeK-at-whiteoaksemi.com
Date: Thu, 24 Feb 2000 14:38:10 -0500
Subject: Job Opening: TEM Technician

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




TEM Technician
A position is available at White Oak Semiconductor, Richmond VA, USA. The
successful candidate will perform TEM operation and sample preparation in
support of DRAM fabrication and TEM engineering. The candidate should have
at least an associates degree and a minimum of 3 years experience in the
semiconductor industry; of which at least 1 year should pertain to
semiconductor TEM sample preparation. Additionally, they should understand
the fundamental tools and techniques of TEM sample preparation and the
semiconductor process from a top down and cross sectional perspective. Good
eye-hand coordination, communication skills, and attention to detail are
required. Candidates should be highly motivated, self directed, good at
problem solving, interested in learning new skills and effective working
alone or in a team environment.
Interested candidates please contact

Kim Christensen
Failure Analysis Laboratory
White Oak Semiconductor
6000 Technology Boulevard
Sandston, Virginia 23150
Ph: 804 952 7307
Fax: 804 952 7902
Pager: 1 800 759 8888, pin# 130 3382



From daemon Thu Feb 24 17:34:12 2000



From: Diana van Driel :      dianavd-at-eye.usyd.edu.au
Date: Fri, 25 Feb 2000 07:56:59 +1100
Subject: epoxy embedding of rat eye

Contents Retrieved from Microscopy Listserver Archives
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Sandy,

If you can puncture the eye, or preferably take off the anterior
chamber, so that the chemicals can get in, there is absolutely no
problem. Standard embedding, using longish times, works well. For
instance, 3 hrs in osmium, 1 hr in UAc, 20 mins in each of the
alcohols/acetone, 1 hr in resin/acetone (dilute), overnight
infiltration in resin/acetone (more concentrated), 1 hr in warm
resin, embed. If you need to have the eye unpunctured, it may be
difficult. I've never embedded a discrete eye, but I do know that it
is really difficult to get even isolated sclera well embedded, as it
seems to act as a very effective barrier to resin. The eye is a well
designed ball that doesn't want to deflate, but that also means
nothing much will get in.

Contact me if you want further info. I've embedded more eyes than I
care to remember, including some rats eyes.

Diana



Diana van Driel
Department of Clinical Ophthalmology
Sydney University
GPO Box 4337
Sydney NSW
AUSTRALIA 2001

Phone 61 2 938 27278/27395
Mob 0412 165 075
Fax 61 2 938 27318



From daemon Thu Feb 24 17:34:16 2000



From: David Hoyle :      dhoyle-at-istar.ca
Date: Thu, 24 Feb 2000 16:15:16 -0800
Subject: Cooling water/glycol Danger

Contents Retrieved from Microscopy Listserver Archives
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Carl,

You are correct Carl you can use glycol to cool your sem(all Hitachi sems)
as only the diffusion pump is being cooled and Hitachi uses a vinyl hose
that isn't effected by the Glycol. For those that have Hitachi TEM's the
water lines for the lenses and diffusion pumps are the smaller black
appearing lines, these will be adversely effected by the glycol.

Sorry for the confusion I will be more specific in the future. So in the
sem's that use the clear reinforced vinyl hoses only, glycol is okay. In
Hitachi TEM which use a small black plastic hose for the lenses and
diffusion pumps, do not use glycol.



} OK, now I am concerned.

} What is the real answer? When we had our Hitachi S-3200N installed
} two years ago, the service engineer used an ethylene glycol/water
} solution in the Haskris chiller. And it was pretty strong, too.
} Something like 1:3 glycol:water.

David Hoyle
Nissei Sangyo Canada
http://www.nsctoronto.com/
service-at-nsctoroto.com (general mail)
dhoyle-at-istar.com (direct mail)



From daemon Thu Feb 24 17:34:17 2000



From: Brian Wajdyk :      r49655-at-email.sps.mot.com
Date: Thu, 24 Feb 2000 14:39:06 -0700
Subject: Job announcement: TEM technician

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am pleased to bring to your attention the following TEM technician
position at Motorola's Process and Materials Characterization Laboratory
(PMCL) I encourage all persons interested to respond ASAP.

Position: TEM Technician/S9001P
Employer: Motorola-Semiconductor Products Sector's Process and Materials
Characterization Laboratory (PMCL)
Location: Mesa, AZ
Employment type: Full-time
Employment status: Full-employee (non-contractor)
Number of Positions: 2
Shift: all (compressed)
Relocation: Available

Duties/Responsibilities: Experience in Transmission Electron Microscopy
techniques including basic operation, specimen preparation, maintenance,
and troubleshooting of TEM's. Work effectively as a team player in
multiple projects providing routine and non-routine TEM analysis in
support of semiconductor product manufacturing. Exposure to many
different types of processes and technologies, and working knowledge of
FIB and EDS are a plus.

Specific knowledge: AA degree preferred. A higher or lower
classification will be established depending upon qualification and
experience.

Contact info: Send resume or questions via email to
Brian_Wajdyk-at-email.mot.com or fax to 480-655-4316 C/O Brian Wajdyk.
--
********************************************************************
Brian Wajdyk
Team Leader / Electron Microscopist (FESEM, EDS, SAM)
Motorola - Process and Materials Characterization Laboratory (PMCL)
2200 W. Broadway Rd., Mesa AZ 85202 Mail Drop: M360
Tel: 480-655-4337 Fax: 480-655-4316
Email: brian_wajdyk-at-email.mot.com Pager: 1-800-313-5960
********************************************************************



From daemon Thu Feb 24 17:34:18 2000



From: DakruegerMN1-at-aol.com
Date: Thu, 24 Feb 2000 16:49:33 EST
Subject: image analysis of fibers

Contents Retrieved from Microscopy Listserver Archives
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Image analysis folks,

I am interested in doing some PC based image analysis of fibers and fiber
properties on crimping. Is there anyone doing IA of crimp angle/amplitude or
crimps per inch? Is there commercial IA software that will do these types of
measurements?

Any help would be appreciated. Thanks.

Duane Krueger
7635 Harold Avenue
Golden Valley, Minnesota 55427

612-541-9880 (FAX)
DakruegerMN1-at-aol.com
yanktonson2-at-aol.com



From daemon Thu Feb 24 17:34:21 2000



From: William Tivol :      tivol-at-wadsworth.org
Date: Thu, 24 Feb 2000 18:00:36 -0500
Subject: Re: Cool Prep (Water Coolers)

Contents Retrieved from Microscopy Listserver Archives
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Gordon Couger wrote:

} Glycols won't have near the heat capacity of water. I doubt
} you will find a bug that will live in ethylene glycol but you can
} probably find many that can live in propylene glycol.
}

Dear Gordon,
I've been trying to look up the heat capacity of glycols
in my Handbook of Chemistry and Physics. It doesn't have the
data I want, but it does have the Cp for water (~4 j/gK), butane
(~0.006 j/molK = ~10^-4 j/gK, if I'm reading my cal's and
Cal's right), and 1-propanol (~0.01 j/molK with the same caviat).
} From these, I'd guess Cp for ethylene glycol is ~0.014 j/molK,
which is, indeed, much less than that of water. Propylene
glycol's Cp should be similar.
Yours,
Bill Tivol



From daemon Thu Feb 24 17:34:22 2000



From: William Tivol :      tivol-at-wadsworth.org
Date: Thu, 24 Feb 2000 18:08:15 -0500
Subject: Re: Cool Prep (Water Coolers)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Divakar R wrote:

} A related question from India. We have been using DM (demineralised) water in our JEOL 2000 EX II cooled by a Neslab chiller for the last ten years. This requires change every 6 months approximately. What additives can be used to prevent algae (? it is green and slimy and I am not a biologist) growth? Something obtainable locally (chemical name rather than trade names) will be most useful to me. Thanks very much.

Dear Divakar,
Russell's suggestion of dichlorophene is good--it's also
what we use. To prevent growth in the tubing, we added some
CuSO4--being careful to keep the pH slightly above 7.5.
We have copper tubing in the system, so the Cu++ should not
be a problem, and it will inhibit algal growth. At that pH,
CuSO4 is not too soluble, so go slowly (if at all).
Yours,
Bill Tivol



From daemon Thu Feb 24 17:34:23 2000



From: William Tivol :      tivol-at-wadsworth.org
Date: Thu, 24 Feb 2000 18:15:47 -0500
Subject: Re: Cool Prep (Water Coolers)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Heather A Owen wrote:

Dear Heather,


} A complete replacement of our chiller water (along with the water in the
} scope and lines) was required about a year ago when the water pump in our
} TEM Haskris had to be replaced. Our sevice engineer flushed the system
} using the concentrated hydrogen peroxide as mentioned above, and a truly
} amazing amount of material spewed forth, so much so that small connectors
} to the power supply clogged up a few weeks down the line, causing our HV
} to shut down. Cleaning out the connectors and flushing the system again
} with distilled water took care of the problem.
}
}

We added inline filters--the ones with the fiber cartridges--to
trap all the particulate material in the lines, and over a period of several
months, we probably got as much material as you got all at once. I highly
recommend adding filters.

At the time, we were cautioned (by Haskris, I think) to use distilled

} water and not deionized water in the chillers. Does anyone know why?
}

Hard to say. Deionized water can contain material leached out
of the resin used, and maybe that is bad for the system, while volatiles
(mostly organics, I'd guess) from distillation may not be.
Yours,
Bill Tivol



From daemon Thu Feb 24 17:44:31 2000



From: Andrew McNaughton :      andrew.mcnaughton-at-stonebow.otago.ac.nz
Date: Fri, 25 Feb 2000 12:31:14 +1300
Subject: Reference points in serial sections.

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Hello

A student wishes to take a number of serial sections through a bee brain
mounted in glycerine. The total distance is around 500µm and she would
like to include three reference points which enable the sections to be
correctly orientated throughout the brain. Embedding three small rods
seems to be the most likely option but the material would need to be stable
and able to be cut by a vibratome. Keeping the rods parallel would also be
beneficial.

If anyone has tried this sort of thing or has any suggestions as to what we
could use for the markers I'd appreciate hearing your bright ideas.

Regards

Andrew McNaughton

______________________________________________________________________________
Andrew McNaughton
South Campus Electron Microscope Unit
C/-Department of Anatomy and Structural Biology
School of Medical Sciences
University of Otago
PO Box 913, Dunedin
NEW ZEALAND

Telephone: +64 3 479 7308
Facsimile: +64 3 479 7254

e-mail: andrew.mcnaughton-at-stonebow.otago.ac.nz
______________________________________________________________________________ht




From daemon Thu Feb 24 21:00:05 2000



From: HŒkan Olin :      hakan.olin-at-fy.chalmers.se
Date: Fri, 25 Feb 2000 08:05:38 +0100
Subject: SPM information on the Web - including TEM-STM

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Dear Timothy,

We are using sputter coater in "etch" mode for this purpose. It is doing the
job flawlessly.
I don't know about your coater but I'll give you the settings we are using
here.
The coater has maximum operating voltage of 1400V. The grids are put on
glass plate (so they do not make contact with the lower electrode) and then
the plate is put on the lower electrode. The current we use is 5 mA for 20
seconds (the current depends strongly on the pressure and the voltage). The
pressure is about 20 Pa.

Good luck !

Rado

---------------------------------------------------------------------
Radostin Danev
Laboratory of Ultrastructure Research
National Institute for Physiological Sciences
Myodaiji-cho, Okazaki 444-8585, JAPAN
e-mail: rado-at-nips.ac.jp
---------------------------------------------------------------------
----- Original Message -----
} From: Timothy Schneider {Timothy.Schneider-at-mail.tju.edu}
To: {microscopy-at-sparc5.microscopy.com}
Sent: Thursday, February 24, 2000 4:29 AM


Dear Microscopy users,

I just want to inform you about a new web site
(http://www.nanofactory.com) with some useful information on scanning
probe microscopy (SPM)

There are, for example:
- Weekly update of SPM research articles
- Suggestions of some SPM literature
- Links to many SPM research groups
- Links to almost all SPM companies

There are also some interesting mpeg-movies with TEM-STM measurements of
two tunneling tips jumping into each other.

Best regards,
Hakan Olin
--
Dr Hakan Olin
Physics and Engineering Physics
Chalmers University of Technology
SE-412 96 Goteborg
Sweden
tel. +46-31-772 3338
fax +46-31-772 3367
mobile +46-70-30 88 99 0
e-mail hakan.olin-at-fy.chalmers.se
home page http://fy.chalmers.se/~f4aolin



From daemon Fri Feb 25 08:32:45 2000



From: Wim Jacob :      jacob-at-uia.ua.ac.be
Date: Fri, 25 Feb 2000 11:31:08 +0100
Subject: Re: Food Science Prep.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


An effective way to prevent your cooling system from growing
green or blue-green algae is to ensure that it is totally light-tight.
Algae require light for photosynthesis. The Philips engineers who
installed our CM120 Biotwin recommended we connect up the
chiller with the black-lined vinyl hose used in drinks vending
machines, where microorganism growth in the water lines is
obviously undesirable. The product we used is trademarked
"Aquavend", is opaque white outside and black inside. It appears to
have worked so far, though the ethylene glycol we use would also
discourage algae.
Yours
Chris Jeffree

Date sent: Thu, 24 Feb 2000 18:08:15 -0500
} From: William Tivol {tivol-at-wadsworth.org}
To: microscopy-at-sparc5.microscopy.com


Dear Debby,
Some ten yaers ago we were doing research on sugar syrup in cooperation with a sugar company. They were interested in the stucture of mixtures of sugar syrups. W e had some succes using freeze-fracture and looking at the replicas in the TEM. Of course these were not mixtures of cereals with sugar syrup and the specimens were allowed to be a lot smaller but this could be a suggestion.
Succes !
Wim Jacob
Ctr.of Electron Microscopy
University of Antwerp
Belgium

Debby Sherman wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hi,
} Well I guess we never run out of new challenges when it comes to specimen prep. This one concerns a product that is made of a cereal bound together with a sugar syrup. The investigators want to see how the syrup behaves as to structural integrety over a period of time with and without the addition of various stabilizing components. Information would be collected at the light microscopic level.
} We first thought of cryosectioning followed by examination using LM. However, the product is filled with air pockets and literally disintegrates when microtomed. It is easily cut into pieces at temperatures above freezing as long as the pieces are no thinner than3-4 mmAccempts to infiltrate it with OCT compoound prior to freezing were not satisfactory.
} New thought is to use a low temperature resin to infiltrate the sample followed by UV or chemical polymerization. butThe resulting blocks could then be microtomed at room temperature and imaged. It would be desirable to cut sections of 4-8 µm in thickness which is too thick for an ultramicrotome with glass or diamond knives. Also UV polymerization would probably limit us to very small sample size which may be a problem.
} Any suggestions would be appreciated as to resins to use which have low viscosity, could be used to infiltrate pieces of ~0.5-1cm sq, polymerize at low temperatures (+4 to 0oC range) using UV or chemical polymerization, and are preferably soft enough to section with a metal microtome blade. Any other suggestions of preparations techniques to try would be very appreciated.
}
} Thanks in advance for your input.
} Debby
}
} Debby Sherman, Manager Phone: 765-494-6666
} Microscopy Center in Agriculture FAX: 765-494-5896
} Dept. of Botany & Plant Pathology E-mail: sherman-at-btny.purdue.edu
} Purdue University
} 1057 Whistler Building
} West Lafayette, IN 47907-1057



From daemon Fri Feb 25 08:32:47 2000



From: Wim Jacob :      jacob-at-uia.ua.ac.be
Date: Fri, 25 Feb 2000 11:52:17 +0100
Subject: Re: Can EELS, WDS or FT-IR localize As in Biol. tissues?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Doug,
Why don't you try imaging SIMS? I would not surprised if such instrument(s) are
present at the University of Arizona.
Of course this would restrict the resolution, I gess, to 0.1 - 0.5 micrometer,
with the newer instruments.
Regards
Wim Jacob
Ctr. of Electron Microscopy
University of Antwerp
Belgium

Doug Cromey wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Colleagues,
}
} We are interested in localizing arsenic in biological tissues (toxicology
} research), but have been unable to come up with a technique that will give
} us the cellular localization information we're looking for (sub-cellular
} would be even better).
}
} We have tried TEM with energy dispersive x-ray microanalysis and our
} concentrations are apparently too low, so we are unable to detect arsenic.
}
} A few years ago we looked into using the autometallographic techniques that
} have been published by Dr. Gorm Dansher & his colleagues. We were told
} that the photographic emulsions required were no longer available.
}
} In a brainstorming session yesterday several techniques were bantered
} about, but in truth we are not familiar enough with the techniques to know
} if they would suit our needs. Does EELS have anything to offer, or perhaps
} wavelength dispersive spectoscopy (WDS)? How about some of the new FT-IR
} instruments? Are there any other techniques that might be able to localize
} arsenic and/or indicate its state of oxidation?
}
} Vendors are welcome to reply.
}
} Yours,
} Doug Cromey
} ....................................................................
} : Douglas W. Cromey, M.S. Dept. of Cell Biology & Anatomy :
} : Research Specialist, Principal University of Arizona :
} : (office: AHSC 4212A) P.O. Box 245044 :
} : (voice: 520-626-2824) Tucson, AZ 85724-5044 USA :
} : (FAX: 520-626-2097) (email: doug-cromey-at-ns.arizona.edu):
} :...................................................................:
} http://www.pharmacy.arizona.edu/exp_path.html
} Home of: "Microscopy and Imaging Resources on the WWW"



From daemon Fri Feb 25 18:05:15 2000



From: Shu-You Li :      syli-at-mail.uni-mainz.de
Date: Fri, 25 Feb 2000 15:56:01 +0100
Subject: TEM: a new TEM website.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi, all,

Please visit my new homepage at http://syli.homepage.com at your convinience!
It contains not only my current research work, but also LINKS to useful journal informations, instruction for authors, on-line EELS database, periodic table, physical constants, online TEM course and etc.

I am looking forward to your invaluable suggestions!
Yours sincerely,

Shu-You Li


**************************************************
Shu-You Li, Dr.
Institut fuer Physikalische Chemie
Johannes Guttenberg Universitaet
Jakob-Welder-Weg 11
D-55099 Mainz, Germany

E-mail: syli-at-mail.uni-mainz.de; syli16-at-hotmail.com
Fax: +49-6131-3923768
Tel: +49-6131-3923148(O)
**************************************************






From daemon Fri Feb 25 18:05:20 2000



From: Jill Verlander :      verlaj-at-medicine.ufl.edu
Date: Fri, 25 Feb 2000 14:25:57 -0500
Subject: Immunogold Workshop

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Becky,
I do not know if it is okay in the S4700 to use glycol. If it is working
as in why change?


X-From_: r-holdford-at-ti.com Thu Feb 24 19:53:24 2000


X-From_: Patrick.Kilcran-at-hii-hitachi.com Thu Feb 24 23:55:28 2000
} From: "Kilcran, Patrick-HIIEX" {Patrick.Kilcran-at-hii-hitachi.com}
To: dhoyle-at-istar.ca
Cc: "Lima, Rick-HIIEX" {Rick.Lima-at-hii-hitachi.com}


Dear Researcher:

The University of Florida College of Medicine Electron Microscopy Facility
is hosting a two-day workshop on immunogold technique. The workshop will
include two identical sessions on April 10/11 and 13/14. Dr. Jan
Leunissen from the Aurion Immunogold Reagent & Accessories, an
internationally known expert in the field, will be the instructor for the
workshop. Attached is the program information. If you are interested in
attending this workshop, please contact Dr. Verlander or Ms. Hong Yi at
the addresses provided below. Due to practical considerations, the
workshop will be limited to a maximum of 20 participants for each session.
Therefore, we encourage you to register as soon as possible. If you are
not able to attend, we would appreciate if you could pass the message onto
someone else who might be interested in learning about immunogold
techniques. Thank you very much.

Dr. Jill W. Verlander Reed (workshop faculty)
Director, University of Florida College of Medicine Electron Microscopy
Facility Phone: (352) 846-0820 Fax: (352) 846-3299 verlaj-at-medicine.ufl.edu

Hong Yi (technical coordinator)
Supervisor, Emory Neurology Microscopy Core Laboratory
Phone: (404) 727-8692
Fax: (404) 727-3157
Email: hyi-at-emory.edu


University of Florida, College of Medicine, Electron Microscopy Facility
Aurion Immunogold Reagents & Accessories / Electron Microscopy Sciences

Present
Immunogold Workshop

April 10-14, 2000
Gainesville, FL

WORKSHOP HOST
Dr. Jill Verlander Reed
Director, Electron Microscopy Facility
University of Florida College of Medicine
Phone: (352) 846-0820
Fax: (352) 846-3299
verlaj-at-medicine.ufl.edu

WORKSHOP INSTRUCTORS

Dr. Jan L.M. Leunissen is an internationally known scientist in the field
of ultrastructural localization. He received his Ph.D in molecular cell
biology at the University of Utrecht in the Netherlands. Dr. Leunissen is
the inventor of ultra small colloidal gold probes and also the founder and
president of the company Aurion, which specializes in immunogold reagents
and custom immunogold labeling. He has been invited to many international
microscopy conferences and workshops and is especially experienced in
providing hands-on training. His previous workshops in Europe, Asia, and
the US have been fully attended and very well received.

Dr. Jill W. Verlander is the Director of the Electron Microscopy Facility
for the University of Florida College of Medicine. Dr. Verlander has 15
years of experience in ultrastructural research in kidney and is known
internationally for her work examining structure-function relationships in
renal transport epithelia. Her work has been published in such journals as
the Journal of Clinical Investigation, the American Journal of Physiology,
Journal of the American Society of Nephrology, and Kidney International.
These studies have been accomplished using light microscopic
immunohistochemistry and in situ hybridization, scanning and transmission
electron microscopy, morphometric analyses, and immunogold and
immunoperoxidase cytochemistry at the ultrastructural level.

GENERAL INFORMATION
Objectives
To provide researchers the opportunity to learn the theory and practice of
immunogold labeling To permit participants to process their own samples
using these techniques under expert guidance To promote technology
exchange and research collaboration

Registration Fees
Regular: $250.00
Student: * $150.00

Lodging
Sheraton Gainesville, $69/night
Phone: (352) 373-6721

Rooms will be reserved in the above hotel under the name "UF EM workshop".
Shuttle buses are available between the hotel and UF campus

Enrollment Note
Two sessions (A; April 10-11, B: April 13-14) will be hosted at UF. Each
session will be limited to a maximum of 20 participants. The workshops
will provide samples to those who prefer not to bring their own. Each
participant will be provided with a sample of gold conjugate of their
choice at the end of the workshop.

MAIN CURRICULUM
The properties of gold particles and their protein conjugates.
Theories underlying immunogold labeling protocols.
Silver enhancement of gold particles
Imunogold labeling on a variety of sample preparations for LM.
Immunogold labeling for EM
a. Pre-embedding immunogold labeling using ultra_small gold conjugates
and silver enhancement. b. Post-embedding immunogold labeling using ultra
small and conventional colloidal gold conjugates.

CONTACT PERSON AND TECHNICAL COORDINATOR
Hong Yi
Phone: (404) 727-8692
Fax: (404) 727-3157
Email: hyi-at-emory.edu


Jill Verlander Reed, D.V.M.
Associate Scientist
Office of the Dean
University of Florida College of Medicine
P.O. Box 100215
Gainesville, FL 32610
verlaj-at-medicine.ufl.edu
Phone: (352) 846-0820
Fax: (352) 846-3299



From daemon Fri Feb 25 18:05:26 2000



From: george sibbald :      geos-at-goldrush.com
Date: Fri, 25 Feb 2000 13:53:49 -0700
Subject: AFM Newslatter

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Molecular Imaging is updating our contact information.

If you are interested in scanning probe microscopy, would you please take a
moment and update your information at http://www.molec.com/info/form.html

Thank you

George

_______________________
George Sibbald, President
Molecular Imaging: Technology Leader In Advanced Bio-AFM Systems
9830 South 51 Street, Ste 124A
Phoenix AZ 85044
(480) 753-4311
www.molec.com




From daemon Fri Feb 25 18:05:28 2000



From: Delilah Wood :      wood-at-pw.usda.gov
Date: Fri, 25 Feb 2000 14:03:54 -0800
Subject: Re: Glycol for cooling

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In fact, we have an S-4700 and the service technician told us that there are tons of problems with using glycol in the chillers. We have a Haskris chiller. Apparently, the glycol volatilizes some of the hose components.


De Wood


At 11:01 AM 2/25/2000 -0800, David Hoyle wrote:

} ------------------------------------------------------------------------

} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html

} -----------------------------------------------------------------------.

}

}

} Becky,

} I do not know if it is okay in the S4700 to use glycol. If it is working

} as in why change?

}

}

} X-From_: r-holdford-at-ti.com Thu Feb 24 19:53:24 2000

} Date: Thu, 24 Feb 2000 19:26:10 -0600

} } From: Becky Holdford { {r-holdford-at-ti.com}

} Organization: KFAB Physical Analysis Lab, Texas Instruments, Dallas, TX

} X-Mailer: Mozilla 4.51 [en] (WinNT; U)

} X-Accept-Language: en,en-US,en-GB,uk,ru

} To: David Hoyle { {dhoyle-at-istar.ca}

} Subject: Re: Cooling water/glycol Danger

} X-MIME-Autoconverted: from 8bit to quoted-printable by tower.ti.com id

} TAA19583

}

} David:Ê we have 3 Hitachi S4700's that have the final lenses water-cooled.Ê

} Does the caveat of glycol still hold?Ê We are using distilled water and have

} had no problem so far.

}

} David Hoyle wrote:

}

} } ----------------------------------------

}

}

{bold} ********************************************************************

Delilah F. Wood

Botanist

USDA-ARS-WRRC

800 Buchanan St.

Albany, CA 94710


Tel: 510-559-5653

Fax: 510-559-5818 {/bold}

{/x-rich}



From daemon Sat Feb 26 10:27:48 2000



From: gregory.argentieri-at-pharma.Novartis.com
Date: Fri, 25 Feb 2000 18:08:34 -0600
Subject: Phtographic printers for TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




}
} Looking to find and to determine what is the best printer (photographic and
} archive quality)
} for TEM digital images.
}
} Currently considering Kodak 8670PS, Codonics, or Fuji Printers
} Would be interested in comments from people using photoraphic qualtiy
} printers
} for TEM applications
} thoughts, "next time's" etc.
}
} Thank You
}
} Gregory Argentieri
} Novartis Pharmacuticals Corp
} gregory.argentieri-at-pharma.novartis.com
}
}
}
}




From daemon Sat Feb 26 10:27:48 2000



From: Purdy, Sam :      SPurdy-at-nationalsteel.com
Date: Fri, 25 Feb 2000 18:10:57 -0600
Subject: RE: Food Science Prep.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Debby Sherman
Talk to Mark Cavaleri at 3M. He made a polished cross sectgion of M%M
candies and preserved tyhe sugr layer

Sam Purdy

} ----------
} From: Debby Sherman
} Sent: February 2000 1:17 PM
} To: message to: MSA list
} Subject: Food Science Prep.
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi,
} Well I guess we never run out of new challenges when it comes to
} specimen prep. This one concerns a product that is made of a cereal bound
} together with a sugar syrup. The investigators want to see how the syrup
} behaves as to structural integrety over a period of time with and without
} the addition of various stabilizing components. Information would be
} collected at the light microscopic level.
} We first thought of cryosectioning followed by examination using LM.
} However, the product is filled with air pockets and literally
} disintegrates when microtomed. It is easily cut into pieces at
} temperatures above freezing as long as the pieces are no thinner than3-4
} mmAccempts to infiltrate it with OCT compoound prior to freezing were not
} satisfactory.
} New thought is to use a low temperature resin to infiltrate the sample
} followed by UV or chemical polymerization. butThe resulting blocks could
} then be microtomed at room temperature and imaged. It would be desirable
} to cut sections of 4-8 µm in thickness which is too thick for an
} ultramicrotome with glass or diamond knives. Also UV polymerization would
} probably limit us to very small sample size which may be a problem.
} Any suggestions would be appreciated as to resins to use which have low
} viscosity, could be used to infiltrate pieces of ~0.5-1cm sq, polymerize
} at low temperatures (+4 to 0oC range) using UV or chemical polymerization,
} and are preferably soft enough to section with a metal microtome blade.
} Any other suggestions of preparations techniques to try would be very
} appreciated.
}
} Thanks in advance for your input.
} Debby
}
} Debby Sherman, Manager Phone: 765-494-6666
} Microscopy Center in Agriculture FAX: 765-494-5896
} Dept. of Botany & Plant Pathology E-mail: sherman-at-btny.purdue.edu
} Purdue University
} 1057 Whistler Building
} West Lafayette, IN 47907-1057
}
}




From daemon Sat Feb 26 10:27:49 2000



From: Mei Lie Wong :      wong-at-msg.ucsf.edu
Date: Fri, 25 Feb 2000 16:10:42 -0800
Subject: Fwd: sem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I received this today. If anyone could help, please reply to the sender.

Thanks.

ML

} Date: Fri, 25 Feb 2000 17:14:04 -0600
} From: Howard Hsu {hhsu-at-kumc.edu}
} Organization: KU Medical Center
} X-Accept-Language: en
} MIME-Version: 1.0
} To: wong-at-msg.ucsf.edu
} Subject: sem
}
} I am a researcher at the University of Kansas Med. Center and would like
} study a biological sample for the presence of mineral using SEM
} approach. If you can provide external service on cost or collaborative
} basis, please let me know. Otherwise, I would greatly appreciate if you
} would let me know any academic or industrial sources that provide this
} type of service. Thank you. Howard Hsu, Ph.D.
}
Mei Lie Wong
Electron Microscope Laboratory
Department of Biochemistry
HHMI-UCSF
Ph. 415-476-4441 Fax 415-476-1902
http://util.ucsf.edu/agard/wong/index.html
email wong-at-msg.ucsf.edu



From daemon Sat Feb 26 10:27:49 2000



From: Ronald R. Matias :      rrm-at-skyinet.net
Date: Sat, 26 Feb 2000 08:19:49 +0800
Subject: Need help on EM negative staining

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hello and Help!

We are a hospital based research lab. starting to use the electron
microscope to identify or confirm pathogens that cause gastrointestinal
disturbances. We would like to know if someone out there could recommend
appropriate specific EM protocols (negative staining, processing of the
gastrointestinal contents, etc....) to visualize these pathogens (ranging
from viruses to bacteria and protozoans). We have a Jeol 1010 EM and perform
the routine EM preparations designed to examine tissues.

In the interest of bring quality healthcare to our people, we will be very
much obliged,

ronnie matias



From daemon Sat Feb 26 10:28:10 2000



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Sat, 26 Feb 2000 05:13:31 -0500
Subject: SEM/TEM analytical service request

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Mei Lie Wong wrote:
==================================================
I received this today. If anyone could help, please reply to the sender.

Thanks.

ML

} Date: Fri, 25 Feb 2000 17:14:04 -0600
} From: Howard Hsu {hhsu-at-kumc.edu}
} Organization: KU Medical Center
} X-Accept-Language: en
} MIME-Version: 1.0
} To: wong-at-msg.ucsf.edu
} Subject: sem
}
} I am a researcher at the University of Kansas Med. Center and would like
} study a biological sample for the presence of mineral using SEM
} approach. If you can provide external service on cost or collaborative
} basis, please let me know. Otherwise, I would greatly appreciate if you
} would let me know any academic or industrial sources that provide this
} type of service. Thank you. Howard Hsu, Ph.D.
}
Mei Lie Wong
Electron Microscope Laboratory
Department of Biochemistry
HHMI-UCSF
Ph. 415-476-4441 Fax 415-476-1902
http://util.ucsf.edu/agard/wong/index.html
email wong-at-msg.ucsf.edu
============================================================
Although you have specifically asked about SEM, I am aware only of a TEM
approach for this kind of analysis. We at Structure Probe, Inc. do perform
this kind of work for clients on a commercial basis. You can see an example
of the final sample that is analyzed by TEM on our website at page URL
http://www.2spi.com/catalog/instruments/etchers4.html

The method is pretty much described so anyone really could do with, provided
they did have access by a plasma etcher such as the SPI Plasma Prep II. If
you wanted to do it yourself and were having difficulties, let us know,
perhaps we could give you assistance. We have found that the removal of the
organics is usually quite important in order to remove the source of the
unwanted Bremsstrahlung radiation.

We have heard from persons trying to do this by SEM/EDS, where by the
section is deposited on a polished beryllium mount, and then the organics
etched away but they seem to have not been successful.

Disclaimer: SPI Supplies manufactures the plasma etching equipment needed
to practice this procedure and our Structure Probe® analytical services part
of our business performs this kind of sample preparation and analysis for
clients. We realize there might be other methods for doing this, but we
have favored the approach that works for us.

Chuck
============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.spi.cc
########################
============================================




From daemon Sat Feb 26 15:00:30 2000



From: przybylo :      przybylowicz-at-nac.ac.za
Date: Sat, 26 Feb 2000 22:44:21 +0200
Subject: As in biological tissues

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Doug,


We have some experience in using micro-PIXE (proton induced X-ray emission)
and the so-called nuclear microprobe in toxicological research. It is more
sensitive than EDS and WDS, is very easy to quantify (standardless
technique). In principle you can have detection limits of the order of 1
ppm in point analysis mode. Specimens can be of any thickness and their
actual thickness is assessed by the simultaneous use of the BS technique
(proton backscatering spectrometry). What in my opinion makes it really
attractive for this purpose is its very good scanning capability. Scan sizes
are from ca. 2.5 mm x 2.5 mm down to the beam resolution, which is of the
order of few micrometer (can be down to 1 micrometer or even less, but in
most of our uses few micrometers is good enough).
The sample preparation is similar as for EDS, using cryotechniques to avoid
elemental losses and redistribution.
We have so far done studies on Ni, Mo, Cd. The detection limits for As are
better than for Mo and Cd.
Of course, this technique cannot indicate the state of oxidation, you can
really treat it as a "more sensitive EDS".

If you want more information, I can refer you to our earlier work and
perhaps you might be interested in contacting me.

Best regards

xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx
Dr Wojciech J. Przybylowicz
Materials Research Group
National Accelerator Centre
PO Box 72
Faure 7131 South Africa
E-mail: przybylowicz-at-nac.ac.za
Fax: +27-21-8433543
Phone: +27-21-8433820
http://www.nac.ac.za/IBA/
http://www.nac.ac.za/materials/
xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx



From daemon Sat Feb 26 15:10:25 2000



From: przybylo :      przybylowicz-at-nac.ac.za
Date: Sat, 26 Feb 2000 22:50:42 +0200
Subject: As in biological tissues

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Doug,


We have some experience in using micro-PIXE (proton induced X-ray emission)
and the so-called nuclear microprobe in toxicological research. It is more
sensitive than EDS and WDS, is very easy to quantify. In principle you can
have detection limits of the order of 1 ppm in point analysis mode.
Specimens can be of any thickness and their actual thickness is assessed by
the simultaneous use of the BS technique (proton backscatering
spectrometry). What in my opinion makes it really attractive for this
purpose is its very good scanning capability. Scan sizes are from ca. 2.5 mm
x 2.5 mm down to the beam resolution, which is of the order of few
micrometer (can be down to 1 micrometer or even less, but in most of our
uses few micrometers is good enough).
The sample preparation is similar as for EDS, using cryotechniques to avoid
elemental losses and redistribution.
We have so far done studies on Ni, Mo, Cd. The detection limits for As are
better than for Mo and Cd.
Of course, this technique cannot indicate the state of oxidation, you can
really treat it as a "more sensitive EDS".

If you want more information, I can refer you to our earlier work and
perhaps you might be interested in contacting me.

Best regards

xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx
Dr Wojciech J. Przybylowicz
Materials Research Group
National Accelerator Centre
PO Box 72
Faure 7131 South Africa
E-mail: przybylowicz-at-nac.ac.za
Fax: +27-21-8433543
Phone: +27-21-8433820
http://www.nac.ac.za/IBA/
http://www.nac.ac.za/materials/
xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx



From daemon Mon Feb 28 07:33:33 2000



From: John Catino :      jwcatino-at-concentric.net
Date: Sun, 27 Feb 2000 21:34:39 -0500
Subject: Print Processor for sale

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have a Kodak Royal Print Processor for sale. The unit is in good
working condition. Our lab is going digital.

Please contact me via e-mail if interested.

John





From daemon Mon Feb 28 07:33:33 2000



From: John Catino :      jwcatino-at-concentric.net
Date: Sun, 27 Feb 2000 21:25:36 -0500
Subject: Kodak Royal Print Processor

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have a Kodak Royal Print Processor for sale. The unit is in good
working condition. Our lab is going digital.

Please contact me via e-mail if interested.

John



From daemon Mon Feb 28 07:33:48 2000



From: Hans-Werner Liebsch :      hwl.bioanalytic-at-bluewin.de
Date: Mon, 28 Feb 2000 11:22:52 +0100
Subject: Active Vibration Isolation News

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


An information about a new principle in vibration damping to all
microscopy users, who have problems with particularly low frequency
vibrations from natural environment.

I invite you kindly to visit our home page:
http://www.hwlbioanalytic.com

We show there active vibration isolation systems for various
applications with damping frequencies from 0,6 Hz to 100/200 Hz and load
capacities (depending from system) from max. 90 kg up to 330/660 kg and
max. 1.500 kg.
Thank you for your time.

With kind regards
Hans-Werner Liebsch

--------------------------------------------
HWLbioanalyticSYSTEMS
Hans-Werner Liebsch
Sales Office Scient. Instruments
Weidenstrasse 11
D - 72119 ENTRINGEN
Germany
Tel.: 0049 (0) 7073 916796
Fax : 0049 (0) 7073 916798
e-mail : hwl.bioanalytic-at-bluewin.de
http://www.hwlbioanalytic.com




From daemon Mon Feb 28 08:33:52 2000



From: tracy gales :      tl_gales-at-fccc.edu
Date: Mon, 28 Feb 2000 09:24:29 -0500
Subject: Virology

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ronnie,

We use many of the techniques and the atlas from the following excellent
book.
"EM in Diagnostic Virology" by Doan and Anderson,1987, Cambridge
University Press,
ISBN 0 521 24311 4.

--
Tracy Gales
Fox Chase Cancer Center
Electron Microscopy Facility
7701 Burholme Ave.
Philadelphia PA 19111
Tel. 215-728-2484
Fax 215-728-2412




From daemon Mon Feb 28 10:40:21 2000



From: Louis M Kerr :      lkerr-at-mbl.edu
Date: Mon, 28 Feb 2000 11:12:56 -0500
Subject: TEM Diffraction plate reader

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


A friend recently retired and gave me a TEM diffraction plate reader. This
was made by Ernest F. Fullam, Inc. in the early 1970's. It is still in the
wooden shipping crate, is in unused condition and looks complete except
there is no manual. It is a basic no-frills manual version. It has a high
quality Starrett vernier scale and could be used for measurement of any
electron micrograph, or other type of transparency, or it may be useful for
teaching the history and theory of diffraction pattern measurement.

I would be willing to send it to someone willing to pay the shipping costs.

Thanks,
Louie

Louie Kerr
Research and Education Support Coordinator
Marine Biological Laboratory
7 MBL Street
Woods Hole, MA 02543
508-289-7273
508-540-6902 (FAX)
508-292-0289 (Cell phone)

VISIT OUR WEB SITE:
http://www.mbl.edu




From daemon Mon Feb 28 19:06:50 2000



From: Guoda Lian :      glian0-at-engr.uky.edu
Date: Mon, 28 Feb 2000 12:49:27 -0500 (EST)
Subject: Position Available (fwd)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html





RESEARCH ASSOCIATE

Electron Microscopy Facility

University of Kentucky


The University of Kentucky invites applications for a Research Associate
in the area of electron microscopy. The Electron Microscopy Facility
houses two SEMs, two TEMs one AFM and extensive sample preparation
equipment that are used by undergraduate and graduate students in
support of research and education. The primary responsibility of the
Research Associate will be to oversee the JEOL 2010F Field Emission TEM
and auxiliary equipment, including an Oxford energy dispersive x-ray
spectrometer (EDS), Gatan electron energy loss imaging filter and
acquisition hardware and software. S/he will be responsible for
coordinating and administering maintenance, access, training and use of
the equipment by internal and external users. S/he will also be
encouraged to develop sponsored research programs and collaborate with
university faculty members. The qualified candidate will have a Ph.D.
and practical experience in analytical electron microscopy and
demonstrate good communication skills. The salary will be commensurate
with qualifications and experience. The University of Kentucky offers
comprehensive insurance and benefits packages and is an equal
opportunity employer. Please send applications to:


Professor Elizabeth Dickey

Director, Electron Microscopy Facility

University of Kentucky

A254 ASTeCC Bldg.

Lexington, KY 40506-0286



***********

Elizabeth C. Dickey

Assistant Professor

Department of Chemical and Materials Engineering

Director, Electron Microscopy Facility

University of Kentucky

177 Anderson Hall

Lexington, KY 40506-0046

ph: 606.257.2300 ext.288

FAX: 606.323.1929

http://www.engr.uky.edu/CME/faculty/dickey/





From daemon Mon Feb 28 19:06:50 2000



From: Sara Miller :      saram-at-duke.edu
Date: Mon, 28 Feb 2000 12:38:41 -0500 (EST)
Subject: Re: Need help on EM negative staining

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ronnie,

We perform about 1000 exams/yr for viruses using EM. Below are some refs
(check out especially 2, 5, 9, 11, 14). Also, at the bottom is a
synopsis I give to our medical students; maybe it will be useful.

As for enteric viruses, EM is the best way to look for them. Many of
them do not grow in tissue culture, and those that can be coaxed, do so
under special conditions that are not employed in the routine culture
lab. Biochemical identification (e.g., immunological kits, PCR, Western
Blots) require a specific reagent to recognize the virus and if you
don't use the right reagent, you will miss viruses (e.g., if you run a
test for rotavirus, you will miss adenovirus, etc.) Furthermore, there
aren't biochemical reagents for all viruses. With EM, you can
see a wide variety of viruses by doing a negative stain of an aqueous
extract of stool.

Good luck,
Sara

REFERENCES ON VIRUS IDENTIFICATION BY ELECTRON MICROSCOPY

1. Cegielski JP, Msengi AE, Miller SE. 1994. Enteric viruses
associated with HIV infection in Tanzanian children with chronic
diarrhea. Ped AIDS HIV Inf 5:296-299.

2. Doane FW, Anderson N. 1986. Electron Microscopy in Diagnostic
Virology: A Practical Guide and Atlas, Cambridge University Press, New York.

3. Lew JF, Glass, RI, Petric M, LeBaron CW, Hammond GW, Miller SE,
Robinson C, Boutilier J, Riepenhoff-Talty M, Payne CM, Franklin R, Oshiro
LS, and Jaqua M-J. 1990. Six year retrospective surveillance of
gastroenteritis viruses identified at ten electron microscopy centers in
the United States and Canada Pediatr Infect Dis J 9:709-714.

4. Mclean DM, Wong KK. 1984. Same Day Diagnosis of Human Virus
Infections, CRC Press, Boca Raton, FL.

5. Miller SE. 1986. Detection and identification of viruses by
electron microscopy. J Electron Microsc Tech 4:265-301.

5. Miller SE, Howell DN. 1988. Viral infections in the acquired
immunodeficiency syndrome. J Electron Microsc Tech 8:41-78.

7. Miller SE. 1988. Diagnostic virology by electron microscopy.
ASM News 54:475-481.

8. Miller SE. 1989. Electron microscopy in rapid viral diagnosis.
EMSA Bull 19:53-59.

9. Hayat MA, Miller SE. 1990. Negative Staining: Applications and
Methods. McGraw-Hill, New York.

10. Miller SE. 1991. Evaluation of electron microscopic information
available from clinical samples. In de la Maza LM, Peterson EM (eds.),
Medical Virology, Vol 10. Irvine, CA. pp 21-54.

11. Miller SE. 1995. Diagnosis of viral infection by electron
microscopy. In Lennette EH, Lennette DA, Lennette ET (eds), Diagnostic
Procedures for Viral, Rickettsial and Chlamydial Infections, American
Public Health Assoc, Washington, DC. pp 35-76.

12. Oshiro LS, Miller SE. 1992. Application of electron microscopy
to the diagnosis of viral infections. In Lennette EH (ed), Laboratory
Diagnosis of Viral Infections. Marcel Dekker, New York. pp 45-68.

13. Palmer E, Martin ML. 1982. An Atlas of Mammalian Viruses, CRC
Press, Boca Raton, FL.

14. Palmer E, Martin ML. 1988. Electron Microscopy in Viral
Diagnosis, CRC Press, Boca Raton, FL.

15. Murphy FA, Fauquet CM, Bishop DHL, Ghabrial SA, Jarvis AW, GP
Martelli, Mayo MA, Summers MD. 1995. Virus Taxonomy. Sixth Report of the
International Committee on Taxonomy of Viruses. Springer-Verlag, New York.

2, 5, 9, 11-14 = Methods and micrographs
2, 14 = Excellent atlases
7, 8 = Brief synopses of technique and basis of virus ID
15 = Good general reference, but don't memorize!! Lists all viruses, not
just human.



TWO WAYS TO LOOK AT VIRUSES BY EM:
Negative staining
Used with liquid samples
Shows whole virus (size, surface structure important)
Thin sectioning
Used with cells and tissues
Shows a slice of an infected cell
Shows relationship of viruses to cell organelles (internal
structure and cell organelle association important)
Location within the cell is a clue to nucleic acid type
DNA viruses are usually seen in the nucleus (some
exceptions).
RNA viruses are usually seen in the cytoplasm
(some exceptions).
Exceptions:
Naked viruses get out of the cell by lysis; if
the cell is very sick, there may be virions
everywhere, e.g., adenovirus (DNA).
Poxviruses are DNA viruses, but are constructed
in the cytoplasm.
Hepatitis B core particles (DNA) may be in the
nucleus and cytoplasm. Dane particles
(complete viruses) are only cytoplasmic.
Some paramyxovirus (RNA) nucleocapsids, not the
complete virion, can be seen in the nucleus
(e.g., measlesvirus).
Enveloped viruses are associated with cell membranes; the
type of cell membrane (plasma membrane,
nuclear membrane, endoplasmic reticulum, vacuoles) can
be a clue to virus identification.

SUMMARY OF VIRUS TYPES (based on morphology):
Naked viruses
All human naked viruses are icosahedral (spherical).
There are 3 size ranges from 20-35 nm, 45-55 nm, 70-90 nm. Examples:
Small: parvovirus, enterovirus, rhinovirus
Medium: polyomavirus, papillomavirus
Large: rotavirus/reovirus, adenovirus
Enveloped viruses
All have a lipoprotein membrane on the outside of the
nucleocapsid. Most derive this layer by budding through
cell membranes; this pliable layer makes the virion
pleomorphic. Poxviruses synthesize their shell de novo;
sometimes they pick up an extra covering by budding. They are
not pleomorphic, but different varieties may be more ovoid,
others, brick-shaped.
Most range in size from 40-400 nm. (Filoviruses are 80 nm by up
to 1400 nm.)
The envelope has proteins on the outside that may look like
spikes or fuzz (e.g., influenza virus, coronavirus).
Sometimes the spikes are too short to be distinguished by EM,
making the virion appear smooth (e.g., herpesviruses,
rubella virus).
The nucleocapsid shape can be
a. icosahedral, like the naked viruses; however, some
are morphologically nondescript as seen by EM;
b. filamentous, like a "slinky."
c. complex (poxvirus cores look like dumbbells with
lateral bodies)
d. morphologically nondescript (just dense without any
particular shape).
Enveloped DNA viruses (except pox) construct their nucleocapsids
in the nucleus.
Enveloped RNA viruses construct their nucleocapsids in the
cytoplasm; (measles nucleocapsids can sometimes be seen
in the nucleus).



On Sat, 26 Feb 2000, Ronald R. Matias wrote:

} Date: Sat, 26 Feb 2000 08:19:49 +0800
} From: Ronald R. Matias {rrm-at-skyinet.net}
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Need help on EM negative staining
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} Hello and Help!
}
} We are a hospital based research lab. starting to use the electron
} microscope to identify or confirm pathogens that cause gastrointestinal
} disturbances. We would like to know if someone out there could recommend
} appropriate specific EM protocols (negative staining, processing of the
} gastrointestinal contents, etc....) to visualize these pathogens (ranging
} from viruses to bacteria and protozoans). We have a Jeol 1010 EM and perform
} the routine EM preparations designed to examine tissues.
}
} In the interest of bring quality healthcare to our people, we will be very
} much obliged,
}
} ronnie matias
}
}

Sara E. Miller, Ph. D.
P. O. Box 3020
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-8735



From daemon Mon Feb 28 19:06:50 2000



From: Sara Miller :      saram-at-duke.edu
Date: Mon, 28 Feb 2000 12:38:41 -0500 (EST)
Subject: Re: Need help on EM negative staining

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




---------- Forwarded message ----------


Ronnie,

We perform about 1000 exams/yr for viruses using EM. Below are some refs
(check out especially 2, 5, 9, 11, 14). Also, at the bottom is a
synopsis I give to our medical students; maybe it will be useful.

As for enteric viruses, EM is the best way to look for them. Many of
them do not grow in tissue culture, and those that can be coaxed, do so
under special conditions that are not employed in the routine culture
lab. Biochemical identification (e.g., immunological kits, PCR, Western
Blots) require a specific reagent to recognize the virus and if you
don't use the right reagent, you will miss viruses (e.g., if you run a
test for rotavirus, you will miss adenovirus, etc.) Furthermore, there
aren't biochemical reagents for all viruses. With EM, you can
see a wide variety of viruses by doing a negative stain of an aqueous
extract of stool.

Good luck,
Sara

REFERENCES ON VIRUS IDENTIFICATION BY ELECTRON MICROSCOPY

1. Cegielski JP, Msengi AE, Miller SE. 1994. Enteric viruses
associated with HIV infection in Tanzanian children with chronic
diarrhea. Ped AIDS HIV Inf 5:296-299.

2. Doane FW, Anderson N. 1986. Electron Microscopy in Diagnostic
Virology: A Practical Guide and Atlas, Cambridge University Press, New York.

3. Lew JF, Glass, RI, Petric M, LeBaron CW, Hammond GW, Miller SE,
Robinson C, Boutilier J, Riepenhoff-Talty M, Payne CM, Franklin R, Oshiro
LS, and Jaqua M-J. 1990. Six year retrospective surveillance of
gastroenteritis viruses identified at ten electron microscopy centers in
the United States and Canada Pediatr Infect Dis J 9:709-714.

4. Mclean DM, Wong KK. 1984. Same Day Diagnosis of Human Virus
Infections, CRC Press, Boca Raton, FL.

5. Miller SE. 1986. Detection and identification of viruses by
electron microscopy. J Electron Microsc Tech 4:265-301.

5. Miller SE, Howell DN. 1988. Viral infections in the acquired
immunodeficiency syndrome. J Electron Microsc Tech 8:41-78.

7. Miller SE. 1988. Diagnostic virology by electron microscopy.
ASM News 54:475-481.

8. Miller SE. 1989. Electron microscopy in rapid viral diagnosis.
EMSA Bull 19:53-59.

9. Hayat MA, Miller SE. 1990. Negative Staining: Applications and
Methods. McGraw-Hill, New York.

10. Miller SE. 1991. Evaluation of electron microscopic information
available from clinical samples. In de la Maza LM, Peterson EM (eds.),
Medical Virology, Vol 10. Irvine, CA. pp 21-54.

11. Miller SE. 1995. Diagnosis of viral infection by electron
microscopy. In Lennette EH, Lennette DA, Lennette ET (eds), Diagnostic
Procedures for Viral, Rickettsial and Chlamydial Infections, American
Public Health Assoc, Washington, DC. pp 35-76.

12. Oshiro LS, Miller SE. 1992. Application of electron microscopy
to the diagnosis of viral infections. In Lennette EH (ed), Laboratory
Diagnosis of Viral Infections. Marcel Dekker, New York. pp 45-68.

13. Palmer E, Martin ML. 1982. An Atlas of Mammalian Viruses, CRC
Press, Boca Raton, FL.

14. Palmer E, Martin ML. 1988. Electron Microscopy in Viral
Diagnosis, CRC Press, Boca Raton, FL.

15. Murphy FA, Fauquet CM, Bishop DHL, Ghabrial SA, Jarvis AW, GP
Martelli, Mayo MA, Summers MD. 1995. Virus Taxonomy. Sixth Report of the
International Committee on Taxonomy of Viruses. Springer-Verlag, New York.

2, 5, 9, 11-14 = Methods and micrographs
2, 14 = Excellent atlases
7, 8 = Brief synopses of technique and basis of virus ID
15 = Good general reference, but don't memorize!! Lists all viruses, not
just human.



TWO WAYS TO LOOK AT VIRUSES BY EM:
Negative staining
Used with liquid samples
Shows whole virus (size, surface structure important)
Thin sectioning
Used with cells and tissues
Shows a slice of an infected cell
Shows relationship of viruses to cell organelles (internal
structure and cell organelle association important)
Location within the cell is a clue to nucleic acid type
DNA viruses are usually seen in the nucleus (some
exceptions).
RNA viruses are usually seen in the cytoplasm
(some exceptions).
Exceptions:
Naked viruses get out of the cell by lysis; if
the cell is very sick, there may be virions
everywhere, e.g., adenovirus (DNA).
Poxviruses are DNA viruses, but are constructed
in the cytoplasm.
Hepatitis B core particles (DNA) may be in the
nucleus and cytoplasm. Dane particles
(complete viruses) are only cytoplasmic.
Some paramyxovirus (RNA) nucleocapsids, not the
complete virion, can be seen in the nucleus
(e.g., measlesvirus).
Enveloped viruses are associated with cell membranes; the
type of cell membrane (plasma membrane,
nuclear membrane, endoplasmic reticulum, vacuoles) can
be a clue to virus identification.

SUMMARY OF VIRUS TYPES (based on morphology):
Naked viruses
All human naked viruses are icosahedral (spherical).
There are 3 size ranges from 20-35 nm, 45-55 nm, 70-90 nm. Examples:
Small: parvovirus, enterovirus, rhinovirus
Medium: polyomavirus, papillomavirus
Large: rotavirus/reovirus, adenovirus
Enveloped viruses
All have a lipoprotein membrane on the outside of the
nucleocapsid. Most derive this layer by budding through
cell membranes; this pliable layer makes the virion
pleomorphic. Poxviruses synthesize their shell de novo;
sometimes they pick up an extra covering by budding. They are
not pleomorphic, but different varieties may be more ovoid,
others, brick-shaped.
Most range in size from 40-400 nm. (Filoviruses are 80 nm by up
to 1400 nm.)
The envelope has proteins on the outside that may look like
spikes or fuzz (e.g., influenza virus, coronavirus).
Sometimes the spikes are too short to be distinguished by EM,
making the virion appear smooth (e.g., herpesviruses,
rubella virus).
The nucleocapsid shape can be
a. icosahedral, like the naked viruses; however, some
are morphologically nondescript as seen by EM;
b. filamentous, like a "slinky."
c. complex (poxvirus cores look like dumbbells with
lateral bodies)
d. morphologically nondescript (just dense without any
particular shape).
Enveloped DNA viruses (except pox) construct their nucleocapsids
in the nucleus.
Enveloped RNA viruses construct their nucleocapsids in the
cytoplasm; (measles nucleocapsids can sometimes be seen
in the nucleus).



On Sat, 26 Feb 2000, Ronald R. Matias wrote:

} Date: Sat, 26 Feb 2000 08:19:49 +0800
} From: Ronald R. Matias {rrm-at-skyinet.net}
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Need help on EM negative staining
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} Hello and Help!
}
} We are a hospital based research lab. starting to use the electron
} microscope to identify or confirm pathogens that cause gastrointestinal
} disturbances. We would like to know if someone out there could recommend
} appropriate specific EM protocols (negative staining, processing of the
} gastrointestinal contents, etc....) to visualize these pathogens (ranging
} from viruses to bacteria and protozoans). We have a Jeol 1010 EM and perform
} the routine EM preparations designed to examine tissues.
}
} In the interest of bring quality healthcare to our people, we will be very
} much obliged,
}
} ronnie matias
}
}

Sara E. Miller, Ph. D.
P. O. Box 3020
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-8735




From daemon Mon Feb 28 19:06:51 2000



From: Sara Miller :      saram-at-duke.edu
Date: Mon, 28 Feb 2000 12:38:41 -0500 (EST)
Subject: Re: Need help on EM negative staining

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ronnie,

We perform about 1000 exams/yr for viruses using EM. Below are some refs
(check out especially 2, 5, 9, 11, 14). Also, at the bottom is a
synopsis I give to our medical students; maybe it will be useful.

As for enteric viruses, EM is the best way to look for them. Many of
them do not grow in tissue culture, and those that can be coaxed, do so
under special conditions that are not employed in the routine culture
lab. Biochemical identification (e.g., immunological kits, PCR, Western
Blots) require a specific reagent to recognize the virus and if you
don't use the right reagent, you will miss viruses (e.g., if you run a
test for rotavirus, you will miss adenovirus, etc.) Furthermore, there
aren't biochemical reagents for all viruses. With EM, you can
see a wide variety of viruses by doing a negative stain of an aqueous
extract of stool.

Good luck,
Sara

REFERENCES ON VIRUS IDENTIFICATION BY ELECTRON MICROSCOPY

1. Cegielski JP, Msengi AE, Miller SE. 1994. Enteric viruses
associated with HIV infection in Tanzanian children with chronic
diarrhea. Ped AIDS HIV Inf 5:296-299.

2. Doane FW, Anderson N. 1986. Electron Microscopy in Diagnostic
Virology: A Practical Guide and Atlas, Cambridge University Press, New York.

3. Lew JF, Glass, RI, Petric M, LeBaron CW, Hammond GW, Miller SE,
Robinson C, Boutilier J, Riepenhoff-Talty M, Payne CM, Franklin R, Oshiro
LS, and Jaqua M-J. 1990. Six year retrospective surveillance of
gastroenteritis viruses identified at ten electron microscopy centers in
the United States and Canada Pediatr Infect Dis J 9:709-714.

4. Mclean DM, Wong KK. 1984. Same Day Diagnosis of Human Virus
Infections, CRC Press, Boca Raton, FL.

5. Miller SE. 1986. Detection and identification of viruses by
electron microscopy. J Electron Microsc Tech 4:265-301.

5. Miller SE, Howell DN. 1988. Viral infections in the acquired
immunodeficiency syndrome. J Electron Microsc Tech 8:41-78.

7. Miller SE. 1988. Diagnostic virology by electron microscopy.
ASM News 54:475-481.

8. Miller SE. 1989. Electron microscopy in rapid viral diagnosis.
EMSA Bull 19:53-59.

9. Hayat MA, Miller SE. 1990. Negative Staining: Applications and
Methods. McGraw-Hill, New York.

10. Miller SE. 1991. Evaluation of electron microscopic information
available from clinical samples. In de la Maza LM, Peterson EM (eds.),
Medical Virology, Vol 10. Irvine, CA. pp 21-54.

11. Miller SE. 1995. Diagnosis of viral infection by electron
microscopy. In Lennette EH, Lennette DA, Lennette ET (eds), Diagnostic
Procedures for Viral, Rickettsial and Chlamydial Infections, American
Public Health Assoc, Washington, DC. pp 35-76.

12. Oshiro LS, Miller SE. 1992. Application of electron microscopy
to the diagnosis of viral infections. In Lennette EH (ed), Laboratory
Diagnosis of Viral Infections. Marcel Dekker, New York. pp 45-68.

13. Palmer E, Martin ML. 1982. An Atlas of Mammalian Viruses, CRC
Press, Boca Raton, FL.

14. Palmer E, Martin ML. 1988. Electron Microscopy in Viral
Diagnosis, CRC Press, Boca Raton, FL.

15. Murphy FA, Fauquet CM, Bishop DHL, Ghabrial SA, Jarvis AW, GP
Martelli, Mayo MA, Summers MD. 1995. Virus Taxonomy. Sixth Report of the
International Committee on Taxonomy of Viruses. Springer-Verlag, New York.

2, 5, 9, 11-14 = Methods and micrographs
2, 14 = Excellent atlases
7, 8 = Brief synopses of technique and basis of virus ID
15 = Good general reference, but don't memorize!! Lists all viruses, not
just human.



TWO WAYS TO LOOK AT VIRUSES BY EM:
Negative staining
Used with liquid samples
Shows whole virus (size, surface structure important)
Thin sectioning
Used with cells and tissues
Shows a slice of an infected cell
Shows relationship of viruses to cell organelles (internal
structure and cell organelle association important)
Location within the cell is a clue to nucleic acid type
DNA viruses are usually seen in the nucleus (some
exceptions).
RNA viruses are usually seen in the cytoplasm
(some exceptions).
Exceptions:
Naked viruses get out of the cell by lysis; if
the cell is very sick, there may be virions
everywhere, e.g., adenovirus (DNA).
Poxviruses are DNA viruses, but are constructed
in the cytoplasm.
Hepatitis B core particles (DNA) may be in the
nucleus and cytoplasm. Dane particles
(complete viruses) are only cytoplasmic.
Some paramyxovirus (RNA) nucleocapsids, not the
complete virion, can be seen in the nucleus
(e.g., measlesvirus).
Enveloped viruses are associated with cell membranes; the
type of cell membrane (plasma membrane,
nuclear membrane, endoplasmic reticulum, vacuoles) can
be a clue to virus identification.

SUMMARY OF VIRUS TYPES (based on morphology):
Naked viruses
All human naked viruses are icosahedral (spherical).
There are 3 size ranges from 20-35 nm, 45-55 nm, 70-90 nm. Examples:
Small: parvovirus, enterovirus, rhinovirus
Medium: polyomavirus, papillomavirus
Large: rotavirus/reovirus, adenovirus
Enveloped viruses
All have a lipoprotein membrane on the outside of the
nucleocapsid. Most derive this layer by budding through
cell membranes; this pliable layer makes the virion
pleomorphic. Poxviruses synthesize their shell de novo;
sometimes they pick up an extra covering by budding. They are
not pleomorphic, but different varieties may be more ovoid,
others, brick-shaped.
Most range in size from 40-400 nm. (Filoviruses are 80 nm by up
to 1400 nm.)
The envelope has proteins on the outside that may look like
spikes or fuzz (e.g., influenza virus, coronavirus).
Sometimes the spikes are too short to be distinguished by EM,
making the virion appear smooth (e.g., herpesviruses,
rubella virus).
The nucleocapsid shape can be
a. icosahedral, like the naked viruses; however, some
are morphologically nondescript as seen by EM;
b. filamentous, like a "slinky."
c. complex (poxvirus cores look like dumbbells with
lateral bodies)
d. morphologically nondescript (just dense without any
particular shape).
Enveloped DNA viruses (except pox) construct their nucleocapsids
in the nucleus.
Enveloped RNA viruses construct their nucleocapsids in the
cytoplasm; (measles nucleocapsids can sometimes be seen
in the nucleus).



On Sat, 26 Feb 2000, Ronald R. Matias wrote:

} Date: Sat, 26 Feb 2000 08:19:49 +0800
} From: Ronald R. Matias {rrm-at-skyinet.net}
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Need help on EM negative staining
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} Hello and Help!
}
} We are a hospital based research lab. starting to use the electron
} microscope to identify or confirm pathogens that cause gastrointestinal
} disturbances. We would like to know if someone out there could recommend
} appropriate specific EM protocols (negative staining, processing of the
} gastrointestinal contents, etc....) to visualize these pathogens (ranging
} from viruses to bacteria and protozoans). We have a Jeol 1010 EM and perform
} the routine EM preparations designed to examine tissues.
}
} In the interest of bring quality healthcare to our people, we will be very
} much obliged,
}
} ronnie matias
}
}

Sara E. Miller, Ph. D.
P. O. Box 3020
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-8735




From daemon Mon Feb 28 19:06:52 2000



From: Guoda Lian :      glian0-at-engr.uky.edu
Date: Mon, 28 Feb 2000 12:49:27 -0500 (EST)
Subject: Position Available (fwd)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




---------- Forwarded message ----------




---------- Forwarded message ----------





RESEARCH ASSOCIATE

Electron Microscopy Facility

University of Kentucky


The University of Kentucky invites applications for a Research Associate
in the area of electron microscopy. The Electron Microscopy Facility
houses two SEMs, two TEMs one AFM and extensive sample preparation
equipment that are used by undergraduate and graduate students in
support of research and education. The primary responsibility of the
Research Associate will be to oversee the JEOL 2010F Field Emission TEM
and auxiliary equipment, including an Oxford energy dispersive x-ray
spectrometer (EDS), Gatan electron energy loss imaging filter and
acquisition hardware and software. S/he will be responsible for
coordinating and administering maintenance, access, training and use of
the equipment by internal and external users. S/he will also be
encouraged to develop sponsored research programs and collaborate with
university faculty members. The qualified candidate will have a Ph.D.
and practical experience in analytical electron microscopy and
demonstrate good communication skills. The salary will be commensurate
with qualifications and experience. The University of Kentucky offers
comprehensive insurance and benefits packages and is an equal
opportunity employer. Please send applications to:


Professor Elizabeth Dickey

Director, Electron Microscopy Facility

University of Kentucky

A254 ASTeCC Bldg.

Lexington, KY 40506-0286



***********

Elizabeth C. Dickey

Assistant Professor

Department of Chemical and Materials Engineering

Director, Electron Microscopy Facility

University of Kentucky

177 Anderson Hall

Lexington, KY 40506-0046

ph: 606.257.2300 ext.288

FAX: 606.323.1929

http://www.engr.uky.edu/CME/faculty/dickey/






From daemon Mon Feb 28 19:06:53 2000



From: Guoda Lian :      glian0-at-engr.uky.edu
Date: Mon, 28 Feb 2000 12:49:27 -0500 (EST)
Subject: Position Available (fwd)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html





RESEARCH ASSOCIATE

Electron Microscopy Facility

University of Kentucky


The University of Kentucky invites applications for a Research Associate
in the area of electron microscopy. The Electron Microscopy Facility
houses two SEMs, two TEMs one AFM and extensive sample preparation
equipment that are used by undergraduate and graduate students in
support of research and education. The primary responsibility of the
Research Associate will be to oversee the JEOL 2010F Field Emission TEM
and auxiliary equipment, including an Oxford energy dispersive x-ray
spectrometer (EDS), Gatan electron energy loss imaging filter and
acquisition hardware and software. S/he will be responsible for
coordinating and administering maintenance, access, training and use of
the equipment by internal and external users. S/he will also be
encouraged to develop sponsored research programs and collaborate with
university faculty members. The qualified candidate will have a Ph.D.
and practical experience in analytical electron microscopy and
demonstrate good communication skills. The salary will be commensurate
with qualifications and experience. The University of Kentucky offers
comprehensive insurance and benefits packages and is an equal
opportunity employer. Please send applications to:


Professor Elizabeth Dickey

Director, Electron Microscopy Facility

University of Kentucky

A254 ASTeCC Bldg.

Lexington, KY 40506-0286



***********

Elizabeth C. Dickey

Assistant Professor

Department of Chemical and Materials Engineering

Director, Electron Microscopy Facility

University of Kentucky

177 Anderson Hall

Lexington, KY 40506-0046

ph: 606.257.2300 ext.288

FAX: 606.323.1929

http://www.engr.uky.edu/CME/faculty/dickey/






From daemon Mon Feb 28 19:06:54 2000



From: Michael Bode :      mb-at-soft-imaging.com
Date: Mon, 28 Feb 2000 11:31:04 -0700
Subject: FW: Image analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Supanee,

I don't know if you have received an answer since you posted this
message, but if you'd like, we could have a look at your images and try
to help you. What we need is of course the image(s) and a description of
what you actually want to measure. Please be as specific as you can.
Please send the material to my email address below.

Thanks.

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Exchange Administrator
Sent: Wednesday, February 23, 2000 8:35 PM
To: Michael Bode


Dear all,
I am doing the image analysis of SEM micrographs. The
micrographs contain both dense (light) and air cell (dark) areas. But
two
areas are not discrete. I'm having a difficulty of quantifying those
separated fractions. I wonder if anyone has any ideas of how to
solve the problem.
Looking forward for your suggestions.
Regards,
Supanee



From daemon Mon Feb 28 19:06:55 2000



From: George Lawton :      George.Lawton-at-email.swmed.edu
Date: Mon, 28 Feb 2000 12:48:57 -0600
Subject: TEM - Embedding Problems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Occassionally someone sends a question to the listserver and there is no response. It doesn't happen often so I thought I would send this question again.


Several weeks ago, I was given some inner membranes from E. coli to be run up to Epon and sectioned for TEM. I treated the sample like tissue culture and pelleted the membranes. Fixed in
3% glut in PBS and post-fixed in 1% OsO4. Routine dehydration in alcohols. Embedded in
EMbed 812 with BDMA in Beem Capsules. Left in oven 48 hours. When I tried to section, the tissue was too soft and appeared to not be infiltrated well. Put back into the oven for 2-3 more days. No difference. Thinking I had a neural meltdown while processing the samples, I tried again on fresh samples, paying particular attention to details. I also used new bottles of embedding media. Same results.
The part of the block without the tissue is its usual hardness. The part of the block with the tissue
(the tip of the beem capsule) is soft and tacky.

Has anyone experienced anything like this? Any suggestions on how I can get the sample into epon?

Thanks in advance for any ideas and/or suggestions.

George Lawton
Chief Electron Microscopist
Molecular and Cellular Imaging Facility
UT Southwestern Medical Center at Dallas
5323 Harry Hines Blvd.
Dallas, Tx 75390-9039
Phone: 214-648-7291
eMail: George.Lawton-at-email.swmed.edu



From daemon Mon Feb 28 19:06:59 2000



From: Greg Erdos :      gwe-at-biotech.ufl.edu
Date: Mon, 28 Feb 2000 15:08:21 -0500
Subject: Re: TEM - Embedding Problems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


If you were working with a pellet in the bottom of a tube or BEEm cap, it
is possible that you were not getting good exchange of reagents at the tip
of the tube during dehydration and embedding.
Or is you were centifuging in the final resin mixture before
putting into the oven it is possible that your sample was packed so tightly
that there was not enough resin in it to polymerize properly.


At 12:48 PM 02/28/2000 -0600, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Gregory W. Erdos, Ph.D. Ph. 352-392-1295
Assistant Director, Biotechnology Program
PO Box 110580 Fax:
352-846-0251
University of Florida
Gainesville, FL 32611





From daemon Mon Feb 28 19:07:01 2000



From: Margie Bryant :      mbryant-at-com1.med.usf.edu
Date: Feb.28, 2000
Subject: Leitz Ultrapak incident-Light illuminator

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hello,

We have an old Leitz Orthoplan microscope and are
looking for the Ultrapak incident light illuminator (housing with fixed
ring mirror and interchangeable UO objectives of 4X to 60X primary
magnification). These components have not been produced in many years.
If anyone has this equipment, we would be pleased to pay for it and for
the shipping. Thank you very much!

Sincerely,

Margie Bryant

From: Margie
Bryant {mbryant-at-com1.med.usf.edu



From daemon Mon Feb 28 19:07:01 2000



From: przybylo :      przybylowicz-at-nac.ac.za
Date: Mon, 28 Feb 2000 23:08:46 +0200
Subject: As in biological tissues

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Dear Doug,

We have some experience in using micro-PIXE (proton induced X-ray emission)
and the so-called nuclear microprobe in toxicological research. It is more
sensitive than EDS and WDS, is very easy to quantify (standardless
technique). In principle you can have detection limits of the order of 1
ppm in point analysis mode. Specimens can be of any thickness and their
actual thickness is assessed by the simultaneous use of the BS technique
(proton backscatering spectrometry). What in my opinion makes it really
attractive for this purpose is its very good scanning capability. Scan sizes
are from ca. 2.5 mm x 2.5 mm down to the beam resolution, which is of the
order of few micrometer (can be down to 1 micrometer or even less, but in
most of our uses few micrometers is good enough).
The sample preparation is similar as for EDS, using cryotechniques to avoid
elemental losses and redistribution.
We have so far done studies on Ni, Mo, Cd. The detection limits for As are
better than for Mo and Cd.
Of course, this technique cannot indicate the state of oxidation, you can
really treat it as a "more sensitive EDS".

If you want more information, I can refer you to our earlier work and
perhaps you might be interested in contacting me.

Best regards


xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx
Dr Wojciech J. Przybylowicz
Materials Research Group
National Accelerator Centre
PO Box 72
Faure 7131 South Africa
E-mail: przybylowicz-at-nac.ac.za
Fax: +27-21-8433543
Phone: +27-21-8433820
http://www.nac.ac.za/IBA/
http://www.nac.ac.za/materials/
xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx



From daemon Mon Feb 28 19:07:02 2000



From: rlvaughn-at-unmc.edu
Date: Mon, 28 Feb 2000 15:15:58 -0600
Subject: network printing management

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I seem to recall a discussion of software that was available to keep track
of printing jobs submitted to a network printer for billing purposes? An
argument here for not installing a networked dyesub printer for digital
images is who is going to be responsible for watching who's using it and
how many prints they're making. We have had some problems like this with
the Epson 850 which is on the network and somebody has ran through two
color cartridges in a short time. Thanks for any advice.

Rick Vaughn
rlvaughn-at-unmc.edu



From daemon Mon Feb 28 19:07:07 2000



From: John J. Bozzola :      bozzola-at-siu.edu
Date: Mon, 28 Feb 2000 17:27:59 -0600
Subject: Image Analysis of Spheres

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We are looking for a program (preferably Macintosh-based) that will
automatically measure the diameter of spheres that have been imaged
in a TEM.

Typically, the spheres measure about 3-5 mm on the TEM negative and
often are touching or slightly overlapped. I know that NIH Image
should do this, but I have not been able to get reproduceable data
using this program.

Someone told me about a program called "Bolero", written by some
Spanish university researchers but it is an $8-10K program. Too rich
for our tastes.

Any suggestions?

We do appreciate your suggestions.

John

####################################################################
John J. Bozzola, Ph.D., Director
Micro-Imaging and Analysis Center
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
####################################################################



From daemon Mon Feb 28 19:07:09 2000



From: William McManus :      billemac-at-biology.usu.edu
Date: Mon, 28 Feb 2000 16:36:18 -0700
Subject: bio: peptidoglycan

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does anyone know of a way to label for peptidoglycan at the light or
electron microscope level?

William McManus
Supervisor
Electron Microscopy Facility
Department of Biology
Utah State University
Logan UT 84322-5305

billEMac-at-cc.usu.edu
435-797-1920



From daemon Mon Feb 28 19:07:09 2000



From: Sara Miller :      saram-at-duke.edu
Date: Mon, 28 Feb 2000 18:42:51 -0500 (EST)
Subject: Re: TEM - Embedding Problems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I agree with Greg: sounds like an infiltration problem. Your best bet
is to encase the bugs in agar. One way is to fix briefly (2-5 min) in glut,
then pellet and let sit for a while (1 hr). This glues them together.

Don't resuspend them in the glut or it will fix the walls of the bugs so
that they won't stick together.

Cut off the bottom of the
tube if plastic or scrape out the cells if tube is glass, onto Parafilm
and drain with wedge of filter paper. Divide into 1 cubic mm (or
smaller) portions and cover with molter agar. Cut away any excess.
Wash. Osmicate and embed as you would a piece of tissue.

Other folks have suspended bugs in molter agar and pelleted them through
the agar. You have to do this before the agar cools. Then cut off the
end with the cells.

Don't fix the agar/cells in glut; it will cross-link the agar so that the
subsequent solutions can't get in. The glut left in the bugs after
they're fixed and before they're encased is OK and will wash out.

Hope this helps.

Sara


Sara E. Miller, Ph. D.
P. O. Box 3020
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-8735



From daemon Mon Feb 28 19:07:09 2000



From: DrJohnRuss-at-aol.com
Date: Mon, 28 Feb 2000 18:49:03 EST
Subject: Re: Image Analysis of Spheres

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



In a message dated 2/28/00 11:44:37 PM, bozzola-at-siu.edu writes:

} Typically, the spheres measure about 3-5 mm on the TEM negative and
} often are touching or slightly overlapped. I know that NIH Image
} should do this, but I have not been able to get reproduceable data
} using this program.

If none of the spheres have been cut off in the section so that you have
stereological corrections to make for the missing parts, and you aren't
having problems with thresholding, etc., then I don't see what your problem
is. Use a watershed to separate the touching features, and use the maximum
feret's diameter to estimate the sphere diameter, not the area (which may be
reduced due to overaps). Or you can use the maximum values of the euclidean
distance map (the ultimate eroded point) as the sphere radii and not even
bother with the watershed.



From daemon Mon Feb 28 21:37:56 2000



From: rachel.harding-at-oxinst.co.uk
Date: Mon, 28 Feb 2000 19:10:48 -0600
Subject: info

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,
Is there a web site where I can find more information about quantitative
techniques in microscopy on SEM's?
In particular the XPP correction procedure?

Many thanks
Rachel


____________________________________________________________
Oxford Instruments Analytical, Halifax Road, High Wycombe, Bucks,
HP12 3SE, UK Tel: +44 (0)1494 442255 Fax: +44 (0)1494 524129
http://www.oxford-instruments.com/ We have a 5M email size limit

Unless stated above to be non-confidential, this E-mail and any
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If you have received this E-mail in error please notify us upon receipt
and delete it from your records. Internet communications are not secure
and Oxford Instruments is not responsible for their abuse by third
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____________________________________________________________




From daemon Mon Feb 28 21:38:03 2000



From: mtl :      mtl-at-njcc.com
Date: Fri, 01 Jan 1999 21:33:41 -0400
Subject: Re: Leitz Ultrapak incident-Light illuminator

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


You may want to try Triad Scientific Inc. in Edison, NJ. ,
triadsci-at-webspan.net or (800) 867-6690. When I was there last month to
purchase a vacuum oven, a whole truck lot of optical microscopes and parts
(Leitz, etc.) was being unloaded. Most were still in the original boxes.
J. Roy Nelson
Material Testing Laboratory
Pennington, NJ
mtl-at-njcc.com

Margie Bryant wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Date: Feb.28, 2000
}
} Hello,
}
} We have an old Leitz Orthoplan microscope and are
} looking for the Ultrapak incident light illuminator (housing with fixed
} ring mirror and interchangeable UO objectives of 4X to 60X primary
} magnification). These components have not been produced in many years.
} If anyone has this equipment, we would be pleased to pay for it and for
} the shipping. Thank you very much!
}
} Sincerely,
}
} Margie Bryant
}
} From: Margie
} Bryant {mbryant-at-com1.med.usf.edu



From daemon Mon Feb 28 21:38:05 2000



From: Marilyn Henderson :      marilyn.henderson-at-adelaide.edu.au
Date: Tue, 29 Feb 2000 13:57:34 +1030
Subject: Re: TEM - Embedding Problems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear George,

I've found the same problem sometimes with Epon/Araldite in Eppendorf
tubes. I think the pellet is so tightly bound to the bottom of the tube
by the time 100% resin infiltration is reached (especially if you
centrifuge along the way) that the resin doesn't have good access to the
cells or material at the 'bottom'. I now lift (ease gently) the pellet
off the bottom of the tube with a toothpick to ensure that the media are
totally surrounding the pellet.
Another choice is to cut away all the gooey resin and hope that the
material at the 'top' of the pellet is well infiltrated and polymerized.

Regards,
Marilyn

George Lawton wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Occassionally someone sends a question to the listserver and there is no response. It doesn't happen often so I thought I would send this question again.
}
} Several weeks ago, I was given some inner membranes from E. coli to be run up to Epon and sectioned for TEM. I treated the sample like tissue culture and pelleted the membranes. Fixed in
} 3% glut in PBS and post-fixed in 1% OsO4. Routine dehydration in alcohols. Embedded in
} EMbed 812 with BDMA in Beem Capsules. Left in oven 48 hours. When I tried to section, the tissue was too soft and appeared to not be infiltrated well. Put back into the oven for 2-3 more days. No difference. Thinking I had a neural meltdown while processing the samples, I tried again on fresh samples, paying particular attention to details. I also used new bottles of embedding media. Same results.
} The part of the block without the tissue is its usual hardness. The part of the block with the tissue
} (the tip of the beem capsule) is soft and tacky.
}
} Has anyone experienced anything like this? Any suggestions on how I can get the sample into epon?
}
} Thanks in advance for any ideas and/or suggestions.
}
} George Lawton
} Chief Electron Microscopist
} Molecular and Cellular Imaging Facility
} UT Southwestern Medical Center at Dallas
} 5323 Harry Hines Blvd.
} Dallas, Tx 75390-9039
} Phone: 214-648-7291
} eMail: George.Lawton-at-email.swmed.edu



From daemon Tue Feb 29 07:09:16 2000



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Mon, 28 Feb 2000 18:37:42 -1000 (HST)
Subject: Re: TEM - Embedding Problems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi-

Two things come to mind. First, if processed in a microfuge tube, loosen
the pellet from the tube and make sure all fluids and epoxy mixtures come
into contact with all surfaces (as already recommended). Second, I always
make it a practice to preheat the BEEM capsules and labels in the
embedding oven all day or overnight before use to drive off any
moisture. This really seems to help in our humid environment!

Good luck and aloha,
Tina

Sunny and 80F but with mosquitoes.

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************



From daemon Tue Feb 29 07:39:41 2000



From: Stanley L. Flegler :      flegler-at-pilot.msu.edu
Date: Tue, 29 Feb 2000 08:20:26 -0500
Subject: Re: network printing management

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Rick:
What you want to do is fairly easy if you are using Windows NT. It will
maintain a log of the user name, number of prints, date, name of print job,
etc. I posted instructions to this group in 1997. These instructions were
published in Microscopy and Microanalysis Vol.3, number 6, p. 569. If you
don't have access to this, let me know and I will send the directions.
Stanley L. Flegler, Acting Director
Center for Advanced Microscopy
Michigan State University



From daemon Tue Feb 29 07:39:42 2000



From: mccu5iv2-at-fs2.ee.umist.ac.uk ()
Date: Tue, 29 Feb 2000 07:19:44 -0600
Subject: HREM of semiconductors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Email: mccu5iv2-at-fs2.ee.umist.ac.uk
Name: Imran

School: Vahora

Question: Currently studying for a project on HREM of semiconductors,
namely GaN; I would like to know where I can obtain some good information
on this subject. My lecturer does not have much insight in this area, I
need to study its structure, and I am currently using the Cerius(2)
software package to simulate images of GaN.

thankyou














---------------------------------------------------------------------------




From daemon Tue Feb 29 07:39:42 2000



From: Duncan Waddell :      D.Waddell-at-mailbox.uq.edu.au
Date: Tue, 29 Feb 2000 07:31:01 -0600
Subject: CyberSTEM demonstration

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The Centre for Microscopy & Microanalysis (CMM) at The University of
Queensland will demonstrate its web-based interactive Virtual Electron
Microscopy service this Thursday (March 2, 2000) between 11am and 1pm.
(Queensland Time; GMT +10)

CyberSTEM (WWW accessible Scanning and Transmission Electron Microscopy)
is a service that became officially permanent on the web after Centre
Directors approved funding last week.

URL: http://www.uq.edu.au/nanoworld/online.html

The CMM's videoconference service - begun in 1995 - has evolved into a
webcam-based service that allows unlimited live connections to
microscopy sessions conducted at the Centre. Visitors can interact with
staff during organised sessions via telephone, by chat programs or via
email. (Please note: Live interaction is only available during
specifically organised sessions - direct all other inquiries to the
address/email noted in the signature below.)

Though primarily intended to service paying customers who cannot
physically attend sessions, video streaming from two of their
microscopes (more later they hope) will be regularly accessible by the
general public.

Sincerely,

Duncan

--
************************************************************
Duncan Waddell (BSc)
Senior Scientific Officer
Centre for Microscopy and Microanalysis
The University of Queensland, St. Lucia, Qld, 4072 Australia
Telephone: +61-7-3365-4216
Facsimile: +61-7-3365-4422
WWW: http://www.uq.edu.au/nanoworld/nanohome.html
************************************************************
Any opinion expressed is that of the writer,
and not necessarily that of CMM or of the University.
************************************************************




From daemon Tue Feb 29 07:49:37 2000



From: Maria.Fazio-Zanakis-at-aventis.com
Date: Tue, 29 Feb 2000 07:37:17 -0600
Subject: TEM - Embedding Problems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear George,
In all my experience working up bacteria and bacterial fragments, I have
always, and by the way this is the simplest, filtered all suspended cells on
Millipore filters and processed the samples on the filters. This yielded
excellent results. The fragments, cells stayed on the filters and never
detached.
Filter your suspended fragment solution using a 0.2um filter unit system
attached to a vacuum system. The aspirator of a faucet works great. Rinse
in buffer while on the filter system.
Then remove the filter and gently place in a plastic or glass petri dish.
Again, gently drop fixative over the sample enough to cover your filter and
carefully slice it into your appropriate sizes and process from there.
You will have success. If you have further questions, just give me a call.

Blessings,
Maria

Maria Fazio-Zanakis
Bioimaging and Molecular Histology
Aventis Pharmaceuticals
1-908-231-3357
Fax: 1-908-231-3962
Email: Maria.Fazio-Zanakis-at-Aventis.com


-----Original Message-----
} From: George Lawton [mailto:George.Lawton-at-email.swmed.edu]
Sent: Monday, February 28, 2000 1:49 PM
To: microscopy-at-sparc5.microscopy.com


Occassionally someone sends a question to the listserver and there is no
response. It doesn't happen often so I thought I would send this question
again.


Several weeks ago, I was given some inner membranes from E. coli to be run
up to Epon and sectioned for TEM. I treated the sample like tissue culture
and pelleted the membranes. Fixed in
3% glut in PBS and post-fixed in 1% OsO4. Routine dehydration in alcohols.
Embedded in
EMbed 812 with BDMA in Beem Capsules. Left in oven 48 hours. When I tried
to section, the tissue was too soft and appeared to not be infiltrated well.
Put back into the oven for 2-3 more days. No difference. Thinking I had a
neural meltdown while processing the samples, I tried again on fresh
samples, paying particular attention to details. I also used new bottles of
embedding media. Same results.
The part of the block without the tissue is its usual hardness. The part of
the block with the tissue
(the tip of the beem capsule) is soft and tacky.

Has anyone experienced anything like this? Any suggestions on how I can get
the sample into epon?

Thanks in advance for any ideas and/or suggestions.

George Lawton
Chief Electron Microscopist
Molecular and Cellular Imaging Facility
UT Southwestern Medical Center at Dallas
5323 Harry Hines Blvd.
Dallas, Tx 75390-9039
Phone: 214-648-7291
eMail: George.Lawton-at-email.swmed.edu



From daemon Tue Feb 29 18:24:15 2000



From: Earl Weltmer :      earlw-at-pacbell.net
Date: Tue, 29 Feb 2000 06:23:04 -0800
Subject: S-520

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all,

I have a customer who wants to purchase a Hitachi S-520 (or equivalent)
SEM with EDS.

If anyone has anything, please contact me off line.

Thank You,

Earl Weltmer
(714) 573-9158





From daemon Tue Feb 29 18:24:15 2000



From: Earl Weltmer :      earlw-at-pacbell.net
Date: Tue, 29 Feb 2000 06:23:18 -0800
Subject: S-520

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all,

I have a customer who wants to purchase a Hitachi S-520 (or equivalent)
SEM with EDS.

If anyone has anything, please contact me off line.

Thank You,

Earl Weltmer
(714) 573-9158





From daemon Tue Feb 29 18:24:19 2000



From: Fiona Russell :      fiona.russell-at-gmx.net
Date: Tue, 29 Feb 2000 16:23:06 +0100 (MET)
Subject: Help on: Plant Cell Chromosome Counting

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Can anyone help me?
I am doing research on the plant Achillea millefolium (Yarrow)and I need
to find out the ploidy level of 2 seed sources. I am looking for information
on companies/institutions that will do this work for me and a rough estimate
of the cost.
Many thanks,
Fiona Russell.

--
Sent through Global Message Exchange - http://www.gmx.net



From daemon Tue Feb 29 18:24:29 2000



From: Steve Miller :      smiller-at-ventanamed.com
Date: Tue, 29 Feb 2000 13:37:31 -0700
Subject: unsubscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html





From daemon Tue Feb 29 18:24:31 2000



From: HILDEGARD CROWLEY :      hcrowley-at-du.edu
Date: Tue, 29 Feb 2000 15:20:37 -0700 (MST)
Subject: Oversimplification-Sorry

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,

I recently tried to help out Dr. M. Rohde who was embedding in Spurr's
formulation and attempting to locate an antigen.

I said that Spurr's formulation has no place in immunocytochemistry for
all the reasons I listed then. This is an oversimplification (I am always
harassed for time when trying to answer questions completely.) Let me
tell you that I, myself, worked with a graduate student who used Spurr's
medium, etched for one hour in sodium metaperiodate, and then got
wonderful Au label. The reason for this success was that she had an
antigen that was so sturdy and such plentiful supply that it was a
candidate for the prozone effect. Her same exact protocol (actually mine)
when tried by us in our laboratory was a spectacular failure with our
antigen which we soon discovered was scarce and delicate and needed
amplification up to the point where I wondered whether we were actually
increasing the thickness of the section by amplification methods! Sort of
like painting a wall until the room gets smaller!
At any rate - yes, Spurr's can be used for immunocytochemistry. However,
if at first you don't succeed, don't try again. Go to another epoxy, and
then to an acrylic.
I should not make absolute statements as I did. I did not mean to
discourage those who successfully use Spurr's to abandon it. Besides,
compared to what there is to be known about immunocytochemistry, my amount
of knowledge can be dropped into a thimble with room left to stuff an
entire thumb in after it.

I always reserve the right to be wrong!

Hildy Crowley
Sr. Electron Microscopy Specialist
Dep. of Biol. Sciences
University of Denver
Denver, CO



From daemon Tue Feb 29 18:24:31 2000



From: Karen Zaruba :      kszaruba-at-MMM.COM
Date: Tue, 29 Feb 2000 16:38:26 -0600
Subject: General: Web sign-up

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers,

I remember seeing posts over the years regarding web-based equipment
sign-up systems, but didn't save any of them. Can anyone direct me to
an archived discussion thread, or have current suggestions on a very
simple and easy way to set this up? I want to put this under a dept.
web page on our intranet only, so security is not a major issue. All I
want is a simple calendar that users can view and sign up for time
slots. No retribution if time not used, but must require user's name so
that they can be tracked down for questioning if needed. I have no
experience in HTML programming but am not afraid to learn.

Thanks as always,
Karen

--
Karen S. Zaruba kszaruba-at-mmm.com
3M Company, St. Paul, MN




From daemon Tue Feb 29 18:24:33 2000



From: Karen Zaruba :      kszaruba-at-MMM.COM
Date: Tue, 29 Feb 2000 16:45:18 -0600
Subject: General: Video Printers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Any suggestions on a really good video printer to include with an SEM
purchase? We've had trouble over the years with the standard roll
printers getting paper jams, which required sending out for servicing
(i.e. lots of down time). We're willing to sacrifice some speed for
something reliable. Are there "fast" ( { 1 min. per print) video
printers that use the cut sheet paper?

Thanks,
Karen

P.S. vendors please respond by E-mail (not phone) or dare to face my
wrath.
--
Karen S. Zaruba kszaruba-at-mmm.com
3M Company, St. Paul, MN




From daemon Tue Feb 29 18:24:36 2000



From: Barbara Plowman :      Bplowman-at-sfmail.dental.uop.edu
Date: Tue, 29 Feb 2000 15:09:31 -0800
Subject: subcribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




From daemon Tue Feb 29 18:24:39 2000



From: Ekstrom, Harry :      harry.ekstrom-at-honeywell.com
Date: Tue, 29 Feb 2000 17:11:10 -0700
Subject: Tracor Northern 5500 EDX Units

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have 2 Tracor Northern/Noran TN 5500 EDX systems that need a new home. We
are looking for a possible trade for comparable priced equipment/software.
If anyone is interested, please contact me directly.

Harry Ekstrom
Honeywell Materials Laboratory
Phoenix, AZ
602-231-2744



From daemon Tue Feb 29 23:00:41 2000



From: David Henriks :      Henriks-at-CompuServe.COM
Date: Tue, 29 Feb 2000 19:59:14 -0500
Subject: TEM: Tripod Polisher¨ Workshop - Date

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Sorry for the inconvenience, but we mistakenly scheduled our Tripod
Polisher¨ Workshop over Memorial Day Weekend. While I'm sure that you
would all love to spend your holiday weekend here with us, we felt it
necessary to move the workshop to the following week. The workshop is now
scheduled for June 2- 3, 2000 in San Clemente, CA.

For those of you who have already registered, you should have already been
contacted and given the option of re-booking for the new date or canceling
your reservation. Again, I apologize for the error. Certainly, if anyone
wishes to come and visit us over the Memorial Day Weekend, we will welcome
you with open arms - but you'll have to wait until the following weekend
for the workshop!

Best regards-

David
Writing at 5:12:19 PM on 02/29/2000

***************************************************************************
************************

David Henriks
Vice President TEL: 800-728-2233
(toll free in the USA)
South Bay Technology, Inc. +1-949-492-2600
1120 Via Callejon FAX: +1-949-492-1499
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

***************************************************************************
************************

} } } } } Please visit us at http://www.southbaytech.com { { { { {

Manufacturers of precision sample preparation equipment and supplies for
metallography, crystallography and electron microscopy.





From daemon Wed Mar 01 07:49:36 2000



From: Evex :      evex-at-evex.com
Date: Wed, 1 Mar 2000 06:43:21 -0500
Subject: S-520

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Earl,

Evex Analytical ( www.evex.com ) has a Jeol 840 with an Evex =3D
Microanalysis system ( http://www.evex.com/prod2.htm ) and Active Digital
Imaging System that we would like to replace with a new microscope.

Claudio Tarquinio
Evex Analytical
Microanalysis and Digital Imaging
609-252-9192 T
609-252-9091 F
www.evex.com
info-at-evex.com





From daemon Wed Mar 01 07:49:37 2000



From: Frank Thomas :      thomasf-at-AGC.BIO.NS.CA
Date: Wed, 1 Mar 2000 08:44:30 -0400
Subject: Re: General: Video Printers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




----------
} From: Karen Zaruba {kszaruba-at-MMM.COM}
} To: MSA Listserve {microscopy-at-sparc5.microscopy.com}
} Subject: General: Video Printers
} Date: February 29, 2000 6:45 PM
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Any suggestions on a really good video printer to include with an SEM
} purchase? We've had trouble over the years with the standard roll
} printers getting paper jams, which required sending out for servicing
} (i.e. lots of down time). We're willing to sacrifice some speed for
} something reliable. Are there "fast" ( { 1 min. per print) video
} printers that use the cut sheet paper?
}
} Thanks,
} Karen
}
} P.S. vendors please respond by E-mail (not phone) or dare to face my
} wrath.
} --
} Karen S. Zaruba kszaruba-at-mmm.com
} 3M Company, St. Paul, MN
}

Karen -

We've always been happy with our little Sony UP-811 video printer. It
doesn't give a very big print (about 5 inches wide), rather like a
Polaroid, and not particularly high resolution or anything, but useful as a
"working copy", with the actual image saved to disk. It's dead reliable,
however, having been in continuous service since about 1993, with no
problems that I know of.
No connection with Sony, etc. (wouldn't mind owning some stock, though;-)

F.C. Thomas
MicroAnalysis Facility
Geological Survey of Canada
Dartmouth, Nova Scotia



From daemon Wed Mar 01 07:49:39 2000



From: White, Woody N :      Woody.N.White-at-mcdermott.com
Date: Wed, 1 Mar 2000 07:57:00 -0600
Subject: Re:General: Video Printers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Karen,

I had a Mitsubishi that was reliable, but in general, my feeling is that for
reproducing EM images there are NO good video printers. Between the
(typical)
paper cost and poor resolution, I prefer to print captured digital images.
Though slow, you can get an inkjet printer these days for 1/10 the price.
Faster ones for a bit more...

Woody White



From daemon Wed Mar 01 08:08:39 2000



From: DARRYL GRAY :      ddg99-at-aber.ac.uk
Date: Wed, 1 Mar 2000 07:52:35 -0600
Subject: LTSEM : Looking for Literature

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Colleagues.... Can anyone help this guy? It's not my area.

Nestor
Your Friendly Neighborhood SysOp
------------------------------------------

Dear Dr Zaluzec

I'd be very grateful if you could help me with a problem I'm having.

I'm writing an essay in low temperature scanning electron microscopy in
biology, but I'm finding it difficult to track down up-to-date source
material.

If you could suggest any papers, books or book chapters related to the
subject, it would be a great help. Thanks.

Darryl Gray




From daemon Wed Mar 01 17:51:12 2000



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Wed, 1 Mar 2000 08:24:54 -0600
Subject: LTSEM : Looking for Literature

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Darryl,

The following web site, http://www.emitech.demon.co.uk/, has a downloadable
technical brief on low temperature EM. It talks about Emitech's products,
but also has a discussion of low-temp EM in more general terms. A more
dated, but very useful, reference is the book "Low Temperature Methods in
Biological Electron Microscopy", which is part of the "Practical Methods in
Electron Microscopy" series edited by Audrey Glauert. My copy is dated
1985. I don't know if later editions exist.

Hope this is useful.

Disclaimer: We have no financial interest in Emitech's products, other than
being users of them.

Regards,
Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/




-----Original Message-----
} From: DARRYL GRAY [mailto:ddg99-at-aber.ac.uk]
Sent: Wednesday, March 01, 2000 7:53 AM
To: Microscopy-at-sparc5.microscopy.com


Colleagues.... Can anyone help this guy? It's not my area.

Nestor
Your Friendly Neighborhood SysOp
------------------------------------------

Dear Dr Zaluzec

I'd be very grateful if you could help me with a problem I'm having.

I'm writing an essay in low temperature scanning electron microscopy in
biology, but I'm finding it difficult to track down up-to-date source
material.

If you could suggest any papers, books or book chapters related to the
subject, it would be a great help. Thanks.

Darryl Gray




From daemon Wed Mar 01 17:51:17 2000



From: Karen Zaruba :      kszaruba-at-MMM.COM
Date: Wed, 01 Mar 2000 12:04:57 -0600
Subject: Re: Proteoglycan staining

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Sorry I deleted the original question so I don't remember who posted
it. Then I came across a couple of papers by John E. Scott on staining
for PG's using Cupromeronic Blue. Never tried this myself, so I don't
know how well it works. Here's one reference:

J.E. Scott and M. Haigh, "Identification of specific binding sites for
keratan sulphate proteoglycans and chondroitin-dermatan sulphate
proteoglycans on collagen fibrils in cornea by the use of Cupromeronic
Blue in 'critical-electrolyte-concentration' techniques." Biochem J.
(1988) 253:607-610.

--
Karen S. Zaruba kszaruba-at-mmm.com
3M Company, St. Paul, MN




From daemon Wed Mar 01 17:51:17 2000



From: Ghanem, Antoine :      Antoine.Ghanem-at-solvay.com
Date: Wed, 1 Mar 2000 18:56:48 +0100
Subject: TEM - Staining of PCL and PVDF

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear microscopists,

Does anybody know a recipe for staining the amorphous part of
poly(vinylidene fluoride) and poly (e-caprolactone)? We need to view the
crystalline lamellae of these polymers by TEM.

Thank you in advance for your help.

Antoine

======================
Dr Antoine GHANEM
SOLVAY Research and Technology
Rue de Ransbeek 310
1120 Brussels
Belgium
Phone 32-2-2643422
Fax 32-2-2642055
antoine.ghanem-at-solvay.com
======================



From daemon Wed Mar 01 17:51:18 2000



From: jmkrupp-at-cats.ucsc.edu (Jon Krupp)
Date: Wed, 1 Mar 2000 10:57:57 -0800
Subject: LM, help with hand sectioning

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greetings:

Someone in the lab needs help with hand sectioning Arabidopsis seedling
stems. They are small, less than a mm in dia. and very delicate. She needs
cross sections of the stem from a specific zone to do microscopy and
pigment localization.

I tried it using traditional techniques, but without success. She must have
sections of fresh material for her stains, she says frozen sections don't
work. Any ideas?

Jonathan Krupp
Microscopy & Imaging Lab
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu




From daemon Wed Mar 01 17:51:18 2000



From: Greg Erdos :      gwe-at-biotech.ufl.edu
Date: Wed, 01 Mar 2000 14:41:06 -0500
Subject: Re: LM, help with hand sectioning

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Suspend them in some stiff agarose and try a vibratome. There is also the
old elder pith trick, if you haven't already tried it. There are only a
few of us ancient enough to have ever heard of that one.


At 10:57 AM 03/01/2000 -0800, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Gregory W. Erdos, Ph.D. Ph. 352-392-1295
Assistant Director, Biotechnology Program
PO Box 110580 Fax:
352-846-0251
University of Florida
Gainesville, FL 32611





From daemon Wed Mar 01 17:51:19 2000



From: Tobias Baskin :      BaskinT-at-missouri.edu
Date: Wed, 1 Mar 2000 13:47:11 -0600
Subject: Re: LM, help with hand sectioning

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greetings,
Jonathan Krupp asked:

}
} Someone in the lab needs help with hand sectioning Arabidopsis seedling
} stems. They are small, less than a mm in dia. and very delicate. She needs
} cross sections of the stem from a specific zone to do microscopy and
} pigment localization.
}
} I tried it using traditional techniques, but without success. She must have
} sections of fresh material for her stains, she says frozen sections don't
} work. Any ideas?
}
We have made some headway in a related problem by encasing
the fresh tissue in low melting point agarose, around 3%. The idea
here is to make a cube of agarose with the stem oriented in side. You
can then cut the whole cube with a razor. The agarose doesn't impede
or interfere with most stains. This didn't work perfectly, but it was
better than nothing.

Hope this helps,
Tobias Baskin
_ ____ ^ __ ____ Tobias I. Baskin
/ \ / / \ / \ \ University of Missouri
/ | / / \ \ \ Biological Sciences
/___/ /__ /___ \ \ \__ 109 Tucker Hall
/ / / \ \ \ Columbia, MO 65211-7400 USA
/ / / \ \ \ voice: 573-882-0173
/ /____ / \ \__/ \____ fax: 573-882-0123



From daemon Wed Mar 01 17:51:19 2000



From: Leona Cohen-Gould :      lcgould-at-mail.med.cornell.edu
Date: Wed, 1 Mar 2000 16:21:24 -0400
Subject: Re: Proteoglycan staining

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Here are the most recent posts. I plan to use the calendar from brownbear
but have not actually implemented it yet.

http://www.brownbearsw.com

Date: Thu, 26 Aug 1999 14:45:39 -0400
Reply-To: Confocal Microscopy List {CONFOCAL-at-LISTSERV.ACSU.BUFFALO.EDU}
Sender: Confocal Microscopy List {CONFOCAL-at-LISTSERV.ACSU.BUFFALO.EDU}
} From: Kristi DeCourcy {decourcy-at-MAIL.VT.EDU}


At 12:04 PM -0600 3/1/0, Karen Zaruba wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

************************
You can also try any number of cationic stains (ruthenium red, alcian blue.
safranin-O, etc)at concentration of about 0.1-0.5%in the primary fixation
step. These dyes are electron dense and will bind to the negatively
charged PG's. You can also track down references from the 1970's & early
1980's by Simeonescu & Simeonescu who did a lot of work with these types of
dyes.
I have used them (years ago) to look at the extracellular matrix in heart
muscle.

Lee

Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Confocal Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175




From daemon Wed Mar 01 17:51:25 2000



From: Scott Whittaker :      sdw-at-biotech.ufl.edu
Date: Wed, 01 Mar 2000 17:12:49 -0500
Subject: TEM polymer help

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have a set of samples giving me fits here. This person is trying to
visualize the ruthenim doped into a polymer. I have not had much luck
working with this stuff and was wondering if any of you had any suggestions
to pass along. A description of the sample follows.Here is what I have
tried so far.

She originally brought this in on a glass slide and it is about 100µm
thick. I tried to use backscattering to visualize the Ru metal in the
FESEM. She thought it might be in little islands. No luck.
It was too thick to shoot a beam through with the TEM so I embedded some in
cold LR-White resin and sectioned. It offered enough support to get some
areas thin enough to view through, but I did not see any metal. I suggested
she take the grids to the Materials EM unit here on campus as the have EDS
and are better equipped for this, but they saw nothing either.

I then had her try to spin coat slids,grids,and mica to float off the film,
but she cannot get it to work so now I am back to the slides, scraping
material off, and cold embedding. The last set was too rubbery though and
even in epoxy on a good diamond I was unable to obtain a section of the
material. It just moved out of the way and popped back up with nice resin
sections floating away. I don't get any polymers here and we are almost
exclusively a biologic unit so do any of you have any suggestions??


} Date: Wed, 1 Mar 2000 11:28:21 -0500 (EST)
} From: Joanne Bedlek {bedlek-at-chem.ufl.edu}
} To: Scott Whittaker {sdw-at-biotech.ufl.edu}
} Subject: Re: your mail
}
} Scott,
}
} The polymer is a polydimethylsiloxane. The metal (Ru) is dissolved in
} methylene chloride and added to the silicone fluid. As the polymer cures
} it "hardens" to a rubbery material.
}
} The TEM images you took give us an idea of the polymer distribution. With
} the new films I have, we are interested in the polymer distribution again.
} I
} suspect we will see the same droplet pattern as before. This will be a
} characterization technique for a paper submission.
}
} As for the dispersion technique, MAIC tried EDS. They were not able to
} detect the Ru. They did tell me that there is a higher content of Si on
} the outer sides of the droplets as opposed to the interior areas. This is
} interesting.
}
} I will drop off the new samples either next week or the week after. It
} depends when I get back next Friday.
}
} I redid the one sample that was giving you so much trouble in slicing. I
} hope it has cured better this time.
}
}
} } This may be more of a dispersion thing. Materials should be able to see it
} } if it is in there. You might get with them and see if they have done
} } anything similar as we had no luck with that last set you brought in. I
} } could also post a discussion to the microscopy listserver and see what
} } comes up. I just need to know more about the composition of the samples
} and
} } stuff like that
} }





} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {
GO GATORS
Scott D. Whittaker 218 Carr Hall
EM Technician Gainesville, FL 32610
University Of Florida ph 352-392-1184
ICBR EM Core Lab fax 352-846-0251
sdw-at-biotech.ufl.edu http://www.biotech.ufl.edu/~emcl/
The home of " Tips & Tricks "







From daemon Wed Mar 01 17:51:27 2000



From: Sara Miller :      saram-at-duke.edu
Date: Wed, 1 Mar 2000 18:01:23 -0500 (EST)
Subject: Re: LM, help with hand sectioning

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I agree with using the agar. Try 3% and go up if you need to. If the
stem is soft, you'll be able to cut it with a "Vibratome" or vibrating
microtome. If it is hard, you won't.

Sara

On Wed, 1 Mar 2000, Jon Krupp wrote:

} Date: Wed, 1 Mar 2000 10:57:57 -0800
} From: Jon Krupp {jmkrupp-at-cats.ucsc.edu}
} To: Microscopy-at-sparc5.microscopy.com
} Subject: LM, help with hand sectioning
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Greetings:
}
} Someone in the lab needs help with hand sectioning Arabidopsis seedling
} stems. They are small, less than a mm in dia. and very delicate. She needs
} cross sections of the stem from a specific zone to do microscopy and
} pigment localization.
}
} I tried it using traditional techniques, but without success. She must have
} sections of fresh material for her stains, she says frozen sections don't
} work. Any ideas?
}
} Jonathan Krupp
} Microscopy & Imaging Lab
} University of California
} Santa Cruz, CA 95064
} (831) 459-2477
} jmkrupp-at-cats.ucsc.edu
}
}
}
}

Sara E. Miller, Ph. D.
P. O. Box 3020
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-8735



From daemon Wed Mar 01 18:40:04 2000



From: pfieber-at-elite.net
Date: Wed, 1 Mar 2000 18:33:46 -0600
Subject: Help:Naturpaths using Dark Field Microscopy

Contents Retrieved from Microscopy Listserver Archives
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Email: pfieber-at-elite.net
Name: Paul Fieber

Question: I read a lot of information about Naturpaths using Dark Field
Microscopy for live blood analysis and blood crystallization testing. They
claim they can see nutritional deficiencies as well as disease process
through this type of testing. Is this possible and how accurate are these
tests? Thanks.

---------------------------------------------------------------------------




From daemon Wed Mar 01 18:40:04 2000



From: Richard G. Heller :      dickh-at-alb.edu
Date: Wed, 1 Mar 2000 18:35:10 -0600
Subject: Zeiss 960 SEM

Contents Retrieved from Microscopy Listserver Archives
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We have the opportunity to purchase a used Zeiss 960 SEM for use by
undergraduate biology students in a number of courses and for research.

I would be interested in any comments, advice, etc. about the instrument
and its appropriateness for this type of application, such as
maintenance, resistance to abuse, and ease of use.

Thank you

Dick Heller
Biology
Albright College

dickh-at-alb.edu




From daemon Thu Mar 02 01:08:09 2000



From: Gordon Couger :      gcouger-at-rfdata.net
Date: Wed, 1 Mar 2000 18:42:02 -0600
Subject: Re: LM, help with hand sectioning

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Elder pith being in short supply you might try supporting it
in a fresh carrot. I have had some luck with that in a hand
microtome.

I came across the method in a book from the 30's.

Gordon

Gordon Couger gcouger-at-couger.com

Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00
} From: "Greg Erdos" {gwe-at-biotech.ufl.edu}
}
}
} Suspend them in some stiff agarose and try a vibratome. There is also the
} old elder pith trick, if you haven't already tried it. There are only a
} few of us ancient enough to have ever heard of that one.
} }
} } Someone in the lab needs help with hand sectioning Arabidopsis seedling
} } stems. They are small, less than a mm in dia. and very delicate. She
needs
} } cross sections of the stem from a specific zone to do microscopy and
} } pigment localization.
} }
} } I tried it using traditional techniques, but without success. She must
have
} } sections of fresh material for her stains, she says frozen sections don't
} } work. Any ideas?
} }
} } Jonathan Krupp
} } Microscopy & Imaging Lab
} } University of California
} } Santa Cruz, CA 95064
} } (831) 459-2477
} } jmkrupp-at-cats.ucsc.edu
} }
} }
}
} Gregory W. Erdos, Ph.D. Ph.
352-392-1295
} Assistant Director, Biotechnology Program
} PO Box 110580
Fax:
} 352-846-0251
} University of Florida
} Gainesville, FL 32611
}
}
}




From daemon Thu Mar 02 01:08:17 2000



From: jim :      jim-at-proscitech.com.au
Date: Thu, 2 Mar 2000 09:32:07 +1000
Subject: RE: LM, help with hand sectioning

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Jon, it seems that project is beyond hand sectioning. There is a whole series
of instruments available that use a oscillating or vibrating knife and the best
can section down to 10 micrometers. Buy, beg, borrow don't steal one of those
instruments. The mid to lower price range should do your job.
Disclaimer: ProSciTech sells these instruments
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Thursday, March 02, 2000 4:58 AM, Jon Krupp [SMTP:jmkrupp-at-cats.ucsc.edu]
wrote:
}
} Greetings:
}
} Someone in the lab needs help with hand sectioning Arabidopsis seedling
} stems. They are small, less than a mm in dia. and very delicate. She needs
} cross sections of the stem from a specific zone to do microscopy and
} pigment localization.
}
} I tried it using traditional techniques, but without success. She must have
} sections of fresh material for her stains, she says frozen sections don't
} work. Any ideas?
}
} Jonathan Krupp
} Microscopy & Imaging Lab
} University of California
} Santa Cruz, CA 95064
} (831) 459-2477
} jmkrupp-at-cats.ucsc.edu
}
}



From daemon Thu Mar 02 01:08:19 2000



From: Mel Dickson :      m.dickson-at-unsw.edu.au
Date: Thu, 02 Mar 2000 15:54:30 +1100
Subject: Naturopaths using Dark Field Microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} Question: I read a lot of information about Naturpaths using Dark Field
} Microscopy for live blood analysis and blood crystallization testing. They
} claim they can see nutritional deficiencies as well as disease process
} through this type of testing. Is this possible and how accurate are these
} tests? Thanks.
}
} ---------------------------------------------------------------------------
}



You CAN see red cells. But the Naturopaths are only using this as a hook
to give (pseudo)scientific validity to their diagnosis. It all COMPLETE
HOKUM.
}
}
}



From daemon Thu Mar 02 08:09:35 2000



From: Drouillon, Philippe :      Philippe.Drouillon-at-solvay.com
Date: Thu, 2 Mar 2000 08:57:13 +0100
Subject: Help needed : a question of death or life...for our TEM microscop

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello all,

We are looking for a high voltage measurement cable (cable which links the
high voltage transformer to the measurement resistances) in working order
and dedicated to a Siemens Elmiskop 102 TEM (manufacturing model number
2810)

Here are the specifications of the cable we are looking for : 125 kV - 1.5 m
length

Would it be possible to also give us a cost estimation ?

Thanks in advance

Best regards

\\ _//
X-FERT ! -(-at- -at-)-
-------------------oOO--(_)--Ooo------------
Philippe Drouillon
Solvay Research and Technology
Electron Microscopy and Image Analysis - IT Coordinator
Rue de Ransbeek, 310
B-1120 Brussels (Belgium)
phone : (00 32) 2 264 24 47
mailto:philippe.drouillon-at-solvay.com

--------------------------Oooo.-------------
.oooO ( )
( ) ) /
\ ( (_/
\_)



From daemon Thu Mar 02 08:09:39 2000



From: Ken Tiekotter :      tiekotte-at-up.edu
Date: Thu, 2 Mar 2000 01:20:01 -0800 (PST)
Subject: Re: LM, help with hand sectioning

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


A modern substitute for elder pith is expanded polystyrene foam.
The dense EPS strips sold by Diatome for cleaning diamond knives
are excellent for sectioning very small items like Arabidopsis
stems.
Chris Jeffree

} From: Gordon Couger {gcouger-at-rfdata.net}
To: Jon Krupp {jmkrupp-at-cats.ucsc.edu} , Microscopy-at-sparc5.microscopy.com,
Greg Erdos {gwe-at-biotech.ufl.edu}


Dear Jon,
I have had great success using LR White Medium grade methacrylate for such
specimens as iris ovules, mistletoe/host tissues, and a variety of young
plant material. Although time consuming, these tissue were processed with
osmium tetroxide, rinsed in buffer, and exposed to a 0.025% tannic acid
buffer to retain lipids, starch granules, and other items frequently lost
during dehyration in EtOH.

One other advantage is the variety of staining procedures one can use with
material embedded in LR White. Furthermore, in addition to lipid
stabilization, osmium and tannic acid provide additional contrast to the
sections.

Hope this helps. Any questions? Email me at tiekotte-at-up.edu

Cheers!
-Ken

----------
Ken Tiekotter, Adjunct Professor
The University of Portland
Department of Biology
5000 N. Willamette Blvd.
Portland, OR 97303

On Wed, 1 Mar 2000, Jon Krupp wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Greetings:
}
} Someone in the lab needs help with hand sectioning Arabidopsis seedling
} stems. They are small, less than a mm in dia. and very delicate. She needs
} cross sections of the stem from a specific zone to do microscopy and
} pigment localization.
}
} I tried it using traditional techniques, but without success. She must have
} sections of fresh material for her stains, she says frozen sections don't
} work. Any ideas?
}
} Jonathan Krupp
} Microscopy & Imaging Lab
} University of California
} Santa Cruz, CA 95064
} (831) 459-2477
} jmkrupp-at-cats.ucsc.edu
}
}
}
}



From daemon Thu Mar 02 08:32:19 2000



From: Huggins, Bradley J :      HUGGINBJ-at-BP.com
Date: Thu, 2 Mar 2000 08:03:37 -0600
Subject: RE: TEM polymer help

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} trying to
} visualize the ruthenim doped into a polymer. I have not had much luck...
}
Scott,
Sounds like a job for cryo-ultramicrotome thin-sectioning at approximately
the glass transition temperature of the polymer. This then to be followed
by TEM imaging and EDS analysis of thin-sections, or FESEM-EDS examination
of the "faced-off" polymer block cross-section.

Many rubbery polymers can be stained "enblock" before sectioning, for
example by submersion in a dilute ruthenium tetroxide (RuO4) solution. This
"staining" often aids the sample prep, by hardening the polymer (actually it
initiates some cross-linking) and allowing better sectioning and sometimes
actually enabling ambient microtomy. It sounds like you will not be able to
make use of this technique since you are looking for the Ru distribution in
the polymer.

A great polymer reference is the book, Polymer Microscopy, by Linda Sawyer
and David Grubb (Chapman and Hall).
Good Luck!
Brad Huggins
BP Amoco, Naperville, IL
Microscopy and Microanalysis Lab
630 420-3668

} ----------
}
} From: Scott Whittaker[SMTP:sdw-at-biotech.ufl.edu]
} Sent: Wednesday, March 01, 2000 4:12 PM
} To: microscopy-at-sparc5.microscopy.com
} Subject: TEM polymer help
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I have a set of samples giving me fits here. This person is trying to
} visualize the ruthenim doped into a polymer. I have not had much luck
} working with this stuff and was wondering if any of you had any
} suggestions
} to pass along. A description of the sample follows.Here is what I have
} tried so far.
}
} She originally brought this in on a glass slide and it is about 100µm
} thick. I tried to use backscattering to visualize the Ru metal in the
} FESEM. She thought it might be in little islands. No luck.
} It was too thick to shoot a beam through with the TEM so I embedded some
} in
} cold LR-White resin and sectioned. It offered enough support to get some
} areas thin enough to view through, but I did not see any metal. I
} suggested
} she take the grids to the Materials EM unit here on campus as the have EDS
}
} and are better equipped for this, but they saw nothing either.
}
} I then had her try to spin coat slids,grids,and mica to float off the
} film,
} but she cannot get it to work so now I am back to the slides, scraping
} material off, and cold embedding. The last set was too rubbery though and
} even in epoxy on a good diamond I was unable to obtain a section of the
} material. It just moved out of the way and popped back up with nice resin
} sections floating away. I don't get any polymers here and we are almost
} exclusively a biologic unit so do any of you have any suggestions??
}
}
} } Date: Wed, 1 Mar 2000 11:28:21 -0500 (EST)
} } From: Joanne Bedlek {bedlek-at-chem.ufl.edu}
} } To: Scott Whittaker {sdw-at-biotech.ufl.edu}
} } Subject: Re: your mail
} }
} } Scott,
} }
} } The polymer is a polydimethylsiloxane. The metal (Ru) is dissolved in
} } methylene chloride and added to the silicone fluid. As the polymer cures
} } it "hardens" to a rubbery material.
} }
} } The TEM images you took give us an idea of the polymer distribution.
} With
} } the new films I have, we are interested in the polymer distribution
} again.
} } I
} } suspect we will see the same droplet pattern as before. This will be a
} } characterization technique for a paper submission.
} }
} } As for the dispersion technique, MAIC tried EDS. They were not able to
} } detect the Ru. They did tell me that there is a higher content of Si on
} } the outer sides of the droplets as opposed to the interior areas. This
} is
} } interesting.
} }
} } I will drop off the new samples either next week or the week after. It
} } depends when I get back next Friday.
} }
} } I redid the one sample that was giving you so much trouble in slicing. I
} } hope it has cured better this time.
} }
} }
} } } This may be more of a dispersion thing. Materials should be able to
} see it
} } } if it is in there. You might get with them and see if they have done
} } } anything similar as we had no luck with that last set you brought in.
} I
} } } could also post a discussion to the microscopy listserver and see what
} } } comes up. I just need to know more about the composition of the
} samples
} } and
} } } stuff like that
} } }
}
}
}
}
}
} } {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {
} GO GATORS
} Scott D. Whittaker 218 Carr Hall
} EM Technician Gainesville, FL 32610
} University Of Florida ph 352-392-1184
} ICBR EM Core Lab fax 352-846-0251
} sdw-at-biotech.ufl.edu http://www.biotech.ufl.edu/~emcl/
} The home of " Tips & Tricks "
}
}
}
}
}
}



From daemon Thu Mar 02 19:17:08 2000



From: Gilbert, Charles :      CGilbert-at-carolinas.org
Date: 2 March, 2000 Time: 7:55 AM E-mail:
Subject: bio: peptidoglycan

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We used High- and Low-iron diamine staining in our lab for looking at
glycosaminoglycans several years ago. This is principally EM level. They
can be enhanced with Thiocarbohydrazide-silver proteinate staining. I still
have the protocols that we used. My reference database is at another
location but the ones below should help you get an idea of what is involved
and who did the work. For HID/LID check into the work by Sam Spicer, I
believe he pioneered it. For TCH-SP, the work is by Thiery and speaking
French would be a plus or Sannes.

Hope this helps

Chuck


REFERENCESREFERENCESREFERENCES FOR IRON DIAMINE AND TCH-SP STAINING:

IRON DIAMINE STAININGIRON DIAMINE STAININGIRON DIAMINE STAINING:

Gad, Adel, and B. Sylven. On the nature of the high iron diamine
method for sulfomucins. J Histochem Cytochem. 17:156-60 (1968).

Lev and Spicer. Am. J. Pathol. 46:23 (1965).

Sorvari, Tapani E. Histochemical observations on the role of ferric
chloride in the high-iron diamine technique for localizing sulphated
mucosubstances. Histochem. J. 4:193-204 (1972a).

Sorvari, Tapani E. Binding of ferric ions to nuclei and other tissue sites
in sections stained for sulfated mucosubstances by the high-iron diamine
methods. Stain Technol. 47:245-48 (1972b).

Sorvari, T. E. and H. S. Arvilommi. Some chemical, physical, and
histochemical properties of three diamine fractions obtained by gel
chromatography from the high-iron diamine staining solution used for
localizing sulphated mucosaccharides. Histochem. J. 5:119-30 (1973).

Spicer, S. S. The use of various cationic reagents in histochemical
differentiation of mucopolysaccharides. Am. J. Clin. Pathol. 36:393-407
(1961).

Spicer, S. S. Diamine methods for differentiating mucosubstances
histochemically. J Histochem Cytochem. 13:211-34 (1965).

Spicer, S. S., et al. Ultrastructural visualization of sulphated complex
carbohydrates in blood and epithelial cells with the high-iron diamine
procedure. Histochem. J. 1O:435-52 (1978).

Spicer, S. S. and M. H. Jarrel. Histochemical reaction of an aromatic
diamine with acid groups and periodate engendered aldehydes in
mucopolysaccharides. J Histochem Cytochem. 9:368-79 (1961).

Takagi, Minoru, et al. Ultrastructural localization of acidic
glycoconjugates with the low iron diamine method. J Histochem Cytochem.
30:471-6 (1982).


TCH-SP ENHANCEMENTTCH-SP ENHANCEMENTTCH-SP ENHANCEMENT:

Denys, Francis R., et al. An improved technique to enhance
high-iron diamine reactive sites with thiocarhohydrazide-silver proteinate.
J. Nihon Univ. Sch. Dent. 25(2):103-6 (1983).

Sannes, P.L., et al. Ultrastructural localization of sulfated
complex carbohydrates with a modified iron diamine procedure. J. Histochem.
Cytochem. 27:1108-11 (1979).

Takagi, M., et al. J. Histochem. Cytochem. 30:1179 (1982).

Thiery, J.P. Mise in evidence des polysaccharides sur coupes fines en
microscopie electronique. J. Microscop. 6:987-1018 (1967).

-------------------------------------
Name: Charles Gilbert VOC: (704) 355-5261
Carolinas Medical Center FAX: (704) 355-8424
Dept of Pediatric Research digPager: (704) 355-4088 : 2058
PO Box 32861
Charlotte, NC 28232-2861



Does anyone know of a way to label for peptidoglycan at the light or
electron microscope level?

William McManus
Supervisor
Electron Microscopy Facility
Department of Biology
Utah State University
Logan UT 84322-5305

billEMac-at-cc.usu.edu {mailto:billEMac-at-cc.usu.edu}
435-797-1920



From daemon Thu Mar 02 19:17:09 2000



From: Jochonia Nxumalo :      jnxumalo-at-chipworks.com
Date: Thu, 2 Mar 2000 09:57:56 -0500
Subject: FT-IR Analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Microscopists,

I am new in this discussion groud, my background is in scanning probe
microscopy and mass spectroscopy. At this time I am interested in analysing
plastic materials such as those used in semiconductor packaging. I would
like to use FT-IR. Is this an appropriate direction to take given that some
of my samples are going to be small (tens micrometers). What are the
material limitations of the technique.
Thank you in advance
Jocho


Jochonia N. Nxumalo
Material Science Engineer

Chipworks Inc.
3685 Richmond Road, Suite 500, Ottawa, Ontario, K2H 5B7
Tel:(613) 829-0414 Fax:(613) 829-0515
mailto:jnxumalo-at-chipworks.com



From daemon Thu Mar 02 19:17:09 2000



From: Dave Ward :      dave_ward-at-baxter.com
Date: Thu, 2 Mar 2000 08:07:49 -0800
Subject: Sputter/Cryo Available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


If anyone is need of an Emscope 2000 sputter/cryostage system at an auction
price please see ad #42220 at LabX.com.



From daemon Thu Mar 02 19:17:10 2000



From: Caroline Schooley :      schooley-at-mcn.org
Date: Thu, 2 Mar 2000 09:37:33 -0800 (PST)
Subject: Re: LM, help with hand sectioning

Contents Retrieved from Microscopy Listserver Archives
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Dear Professor Luchtel
My apologies for the confusion - I was writing without reference to
Diatome's literature. EPS is just short for Expanded PolyStyrene
but their polystyrol rods would be the same thing.
I have found these Diatome rods useful for a number of
applications, and that their density, which is a little higher than
usual for commercial polystyrene foam, provides good support to
the specimen when hand sectioning. However, if you want to try it
out on the cheap, why not cut strips off an insulated polystyrene
box or insulation tile.

By the way, the best type of blade for hand sectioning used to be
the Blue Gillette double-edged carbon steel razor blades, which
many of you may be too young to know about. The new, doubtless
improved, stainless steel type never seems to be so sharp, and
their edges dull faster. Is there still a manufacturer of carbon steel
razor blades left in the world, or am I doomed to mourn forever?

Good luck
Chris Jeffree

Date sent: Thu, 2 Mar 2000 07:56:53 -0400
To: "Chris Jeffree" {cjeffree-at-srv0.bio.ed.ac.uk}
} From: Dan Luchtel {dluchtel-at-u.washington.edu}



}
} Someone in the lab needs help with hand sectioning Arabidopsis seedling
} stems. They are small, less than a mm in dia. and very delicate. She needs
} cross sections of the stem from a specific zone to do microscopy and
} pigment localization.
}
} I tried it using traditional techniques, but without success. She must have
} sections of fresh material for her stains, she says frozen sections don't
} work. Any ideas?
}
} Jon -

After you get the stem supported (you've gotten lots of good suggestions),
try taping 2 razor blades together, with a thin spacer (thick paper,
filecard, etc.). Cheaper than a vibratome...

Caroline

Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/PCI/pci.html




From daemon Thu Mar 02 19:17:14 2000



From: Kristen Lennon :      kalen-at-citrus.ucr.edu
Date: Thu, 02 Mar 2000 10:24:45 -0800
Subject: Re: LM, help with hand sectioning

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Ken,
You've spurred a question that is related, yet not exactly on the same
track. Does tannic acid affect the antigenicity of the tissue? I am just
about to embed some tissue in LR White for immunocytological studies, and
though it is established that osmium is a problem for immuno work, I have
heard nothing about tannic acid. I would love to find a way to enhance the
contrast of my tissue, as it often has a surreal, ghostly appearance.
Thanks,
Kristen

At 01:20 AM 3/2/2000 -0800, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Thu Mar 02 19:17:15 2000



From: Corazon Bucana :      bucana-at-audumla.mdacc.tmc.edu
Date: Thu, 02 Mar 2000 13:19:40 -0600
Subject: SEM of DNA array

Contents Retrieved from Microscopy Listserver Archives
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I received a sample of DNA microarray on a glass slide and I was asked to do
SEM because the investigator wants to see single and double stranded DNA.
Does anyone know if this is possible. My previous exposure to EM of DNA
samples was in TEM. I would appreciate any help, comments or suggestions.

Thank you,

Corazon D. Bucana
*******************************************************
Corazon D. Bucana, Ph.D.
Department of Cancer Biology
U.T. M.D. Anderson Cancer Center
1515 Holcombe Blvd. Box 173
Houston, Texas 77030
Phone: (713) 792-8106
FAX: (713) 792-8747
Email:bucana-at-audumla.mdacc.tmc.edu
FAX: (713) 792-8747



From daemon Thu Mar 02 19:17:27 2000



From: erich-at-ento.csiro.au (Eric Hines)
Date: Fri, 3 Mar 2000 11:36:49 +1100
Subject: surplus old equipt - edax

Contents Retrieved from Microscopy Listserver Archives
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Dear all,

The following equipment is surplus to requirements and is available on the
basis that you are responsible for all removal and transport costs. If
there are competing bids they will be sold to the highest bidder. There is
no guarantee that the equipment is in operational or serviceable condition
although, to the best of our knowledge, it was functional when last
operated.


Kevex-ray 5100 spectrometer.
Kevex-ray detector 3203-100-VS/30
Kevex-ray detector 3203-100/30-V

Jeol high resolution SEM attachment for 100C(X).

Balzers freeze fracture/etch (~20y/o) "divers bell" chamber - EVM052
controller model.

Eric Hines
Microscopy Centre
CSIRO Entomology and Plant Industry
Canberra, Australia





From daemon Fri Mar 03 08:20:59 2000



From: Gordon Couger :      gcouger-at-rfdata.net
Date: Thu, 2 Mar 2000 22:34:16 -0600
Subject: Re: LM, help with hand sectioning

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



----- Original Message -----
} From: "Chris Jeffree" {cjeffree-at-srv0.bio.ed.ac.uk}
} By the way, the best type of blade for hand sectioning used to be
} the Blue Gillette double-edged carbon steel razor blades, which
} many of you may be too young to know about. The new, doubtless
} improved, stainless steel type never seems to be so sharp, and
} their edges dull faster. Is there still a manufacturer of carbon steel
} razor blades left in the world, or am I doomed to mourn forever?

I miss the old blades as well. I cured the shaving problem I quit.
For hand sections I have better luck with a more rigid blade.
Razor blades and straight razors tend to dig in for me. I have
a lot better luck with a 2.5 inch wood chisel sharpened on glass
with silicone carbide grit. It is a lot of trouble to get sharp but
it works better for me than razor blades.

Gordon

Gordon Couger gcouger-at-couger.com

Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00




From daemon Fri Mar 03 08:55:51 2000



From: Grazyna M Tokarczyk :      gmtokarc-at-is.dal.ca
Date: Fri, 3 Mar 2000 10:26:10 -0400 (AST)
Subject: Re: hand sectioning - carbon blades

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




On Thu, 2 Mar 2000, Gordon Couger wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
} Yes, carbon blades are still made - one of the makers is a Japanese
company - I have a box in my hand, but unfortunately I can't read Japanese
- the only English words on it is "Feather". There may also be many other
makers of these in Asia and Eastern Europe.

Grazyna Tokarczyk
gmtokarc-at-is.dal.ca
Dalhousie University
Halifax, Canada
}
} ----- Original Message -----
} } From: "Chris Jeffree" {cjeffree-at-srv0.bio.ed.ac.uk}
} } By the way, the best type of blade for hand sectioning used to be
} } the Blue Gillette double-edged carbon steel razor blades, which
} } many of you may be too young to know about. The new, doubtless
} } improved, stainless steel type never seems to be so sharp, and
} } their edges dull faster. Is there still a manufacturer of carbon steel
} } razor blades left in the world, or am I doomed to mourn forever?
}
} I miss the old blades as well. I cured the shaving problem I quit.
} For hand sections I have better luck with a more rigid blade.
} Razor blades and straight razors tend to dig in for me. I have
} a lot better luck with a 2.5 inch wood chisel sharpened on glass
} with silicone carbide grit. It is a lot of trouble to get sharp but
} it works better for me than razor blades.
}
} Gordon
}
} Gordon Couger gcouger-at-couger.com
}
} Stillwater, OK www.couger.com/gcouger
} 405 624-2855 GMT -6:00
}
}
}
}



From daemon Fri Mar 03 08:55:51 2000



From: Mark Sanders :      msanders-at-biosci.cbs.umn.edu
Date: Fri, 3 Mar 2000 08:30:40 -0600
Subject: Balzers 301 freeze fracture

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Please post the message below.

Dear all,
We have a complete Balzers 301 freeze fracture unit with thickness monitor,
many ancillary accessories and spare parts that is in need of a new home.
No guarantees that the equipment is in operational or serviceable condition
but, to the best of our knowledge, it was functional when last used.
 Please contact me directly for additional information.
Cheers, Mark **************************************************** Mark A.
Sanders
           University of
Minnesota Program Director
          Twin Cities
Campus Imaging Center
            St.
Paul, MN  55108 23-35 Snyder Hall
         ph:
 612-624-3454 1475 Gortner Ave.
         fax: 612-624-1799
http://biosci.umn.edu/imagingcenter President: Minnesota Microscopy Society
http://resolution.umn.edu/MMS/




From daemon Fri Mar 03 08:55:51 2000



From: Pierre-Yves Sizaret :      sizaret-at-med.univ-tours.fr
Date: Fri, 3 Mar 2000 08:33:29 -0600
Subject: confocal

Contents Retrieved from Microscopy Listserver Archives
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Dear colleagues,
We are in the process to get a confocal microscope for a multi-user
facility. We are not decided yet between different apparatus such as
Wallac ultraview, zeiss LSM 510 or Biorad.
We would very much appreciate any advice helping us for this choice.

Many thanks.

Pierre-Yves Sizaret
Electron Microscopy Facility
University of Tours
France




From daemon Fri Mar 03 17:47:00 2000



From: David_Bell-at-Millipore.com
Date: Fri, 3 Mar 2000 11:02:50 -0500
Subject: Question Re: REM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Colleagues,

Twice now, I have had customers in Europe refer to a technique as REM. The
sample prep sounds the same as SEM, and the first time I passed it off as a
typo. Now, with this second reference, I'm not so certain. Is it possible
that these customers are referring to SEM, possibly with their native
language? Or is it a whole different technique/instrument? Could someone
please help me clear up this confusion?

Thanks in advance,

David Bell
Scientist
Electron Microscopy Lab
Millipore Corporation
80 Ashby Road
Bedford, MA 01730
(781) 533-2108



From daemon Fri Mar 03 17:47:02 2000



From: Robert Palmer :      rjpalmer-at-dir.nidcr.nih.gov
Date: Fri, 3 Mar 2000 11:56:33 -0500
Subject: Re: Question Re: REM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Raster Elektronen Mikroskopie = Scanning Electron Microscopy

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Robert J. Palmer Jr., Ph.D.
Oral Infection and Immunity Branch
National Institute of Dental and Craniofacial Research
Bldg. 30, Room 308
National Institutes of Health
Bethesda MD 20892
Ph 301-594-0025
FAX 301-402-0396




From daemon Fri Mar 03 17:47:03 2000



From: David Knecht :      knecht-at-uconn.edu
Date: Fri, 3 Mar 2000 12:31:25 -0500
Subject: Fluorescent agar

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Has anyone heard of a fluorescent agar or a way to make agar fluorescent?
We want to make sure the agar can be fluorescently imaged, but the dye
cannot diffuse out into the surrounding media. Thanks- Dave


************************************************************

Dr. David Knecht
Department of Molecular and Cell Biology
University of Connecticut
75 N. Eagleville Rd. U-125
Storrs, CT 06269
Knecht-at-uconnvm.uconn.edu
860-486-2200 860-486-4331 (fax)
home page: http://www.sp.uconn.edu/~mcbstaff/knecht/knecht.html
************************************************************




From daemon Fri Mar 03 17:47:06 2000



From: Anita Garg :      Anita.Garg-at-lerc.nasa.gov
Date: Fri, 3 Mar 2000 13:55:09 -0500
Subject: Re: Composition analysis

Contents Retrieved from Microscopy Listserver Archives
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Dear Colleagues
In a microstructurally uniform alloy, can one expect to get the
chemical composition of the alloy through microprobe (assuming all
the corrections are applied correctly)? Would the composition tally
with the one determined by chemical analysis?
TIA
Anita



From daemon Fri Mar 03 17:47:06 2000



From: Larry :      mishot-at-itsa.ucsf.edu
Date: Fri, 03 Mar 2000 10:55:23 -0800
Subject: confocal

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Confocal issues are discussed on another listserver--see below

The best solution is to get one of each manufacturer's confocal systems!
Each one has problems and each one has solutions. In my experience the
Wallac system is not sensitive enough for some of our samples but is great
for live imaging. The BioRad 1024 is difficult because of their interface
to the Nikon TE300 and their use of an old OS2 operating system (the
migration to Windows NT is still in the future) but they have a good
service organization in our area and there are many other users that can
help with advice. I do not have hands on experience with the Leica, Zeiss,
Noran and other systems. They each have attractive features. A recent
discussion of Zeiss and Leica will be sent directly to you. Make a list of
features/advantages/disadvantages and weight them--the results may surprise
you and guide your decision.

to subscribe to the confocal newslist send an email to:

{LISTSERV-at-LISTSERV.ACSU.BUFFALO.EDU}

in the body of the message write:

SUBSCRIBE CONFOCAL your name


Larry D. Ackerman
Lily & Yuh Nung Jan Laboratories
Howard Hughes Medical Institute
UCSF, Box 0725, Rm U226
533 Parnassus Ave.
San Francisco, CA 94143

(415) 476-8751 FAX (415) 476-5774
mishot-at-itsa.ucsf.edu



From daemon Fri Mar 03 17:47:07 2000



From: David_Bell-at-Millipore.com
Date: Fri, 3 Mar 2000 14:20:07 -0500
Subject: Re: Question Re: REM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hi all,

Thanks for a quick and consice response!!

David



From daemon Fri Mar 03 17:47:09 2000



From: Michael Bode :      mb-at-soft-imaging.com
Date: Fri, 3 Mar 2000 12:38:51 -0700
Subject: RE: Question Re: REM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


It's exactly the same thing:

Scanning Electron Microscopy (English)
RasterElektronenMikroskopie (German)

(It's 3 long words in English, one monumental word in German. I once
gave a talk titled: "Rasterelektronenmikroskopische
Untersuchungsmethoden fuer prozessinduzierte Halbleiterdefekte"....
German is not for the timid ;-)

Michael

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



} ----------
} From:
"David_Bell-at-Millipore.com"-at-sparc5.microscopy.com[SMTP:"DAVID_BELL-at-MILLIP
ORE.COM"-at-SPARC5.MICROSCOPY.COM]
} Sent: Friday, March 03, 2000 9:02:50 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Question Re: REM
} Auto forwarded by a Rule
}
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Hello Colleagues,

Twice now, I have had customers in Europe refer to a technique as REM.
The
sample prep sounds the same as SEM, and the first time I passed it off
as a
typo. Now, with this second reference, I'm not so certain. Is it
possible
that these customers are referring to SEM, possibly with their native
language? Or is it a whole different technique/instrument? Could
someone
please help me clear up this confusion?

Thanks in advance,

David Bell
Scientist
Electron Microscopy Lab
Millipore Corporation
80 Ashby Road
Bedford, MA 01730
(781) 533-2108



From daemon Fri Mar 03 17:47:12 2000



From: Ken Tiekotter :      tiekotte-at-up.edu
Date: Fri, 3 Mar 2000 12:47:58 -0800 (PST)
Subject: Re: LM, help with hand sectioning

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


For those who are 'hand sectioning', try the teflon coated single edged
blades. I believe Ted Pella and EMS carry them. They are alittle more
money, but they slide through tissue with great ease.

Cheers,
Ken

On Thu, 2 Mar 2000, Gordon Couger wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} ----- Original Message -----
} } From: "Chris Jeffree" {cjeffree-at-srv0.bio.ed.ac.uk}
} } By the way, the best type of blade for hand sectioning used to be
} } the Blue Gillette double-edged carbon steel razor blades, which
} } many of you may be too young to know about. The new, doubtless
} } improved, stainless steel type never seems to be so sharp, and
} } their edges dull faster. Is there still a manufacturer of carbon steel
} } razor blades left in the world, or am I doomed to mourn forever?
}
} I miss the old blades as well. I cured the shaving problem I quit.
} For hand sections I have better luck with a more rigid blade.
} Razor blades and straight razors tend to dig in for me. I have
} a lot better luck with a 2.5 inch wood chisel sharpened on glass
} with silicone carbide grit. It is a lot of trouble to get sharp but
} it works better for me than razor blades.
}
} Gordon
}
} Gordon Couger gcouger-at-couger.com
}
} Stillwater, OK www.couger.com/gcouger
} 405 624-2855 GMT -6:00
}
}
}
}



From daemon Fri Mar 03 17:47:16 2000



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Fri, 03 Mar 2000 15:54:34 -0600
Subject: Re: Composition analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In short, certainly!

The issue might only be are all the elements "visible" to the x-ray
analyzer. I don't trust the C or N numbers that my system spits out. Since
I am using EDS, I will usually trust my careful analyses to a few tenths of
a percent. I can see down to about a tenth of a percent, but the relative
errors are large. But it is usually well sufficient for determining the alloy.

Now just because the alloy is microstructurally uniform, do not count on
the composition to be uniform. I have seen zoning in grains and changes in
composition with length within single crystal ingots. And if you have
multiphase microstructure, it can be challenging to determine to overall
composition.

Warren S.

At 01:55 PM 3/3/2000 -0500, you wrote:

} Dear Colleagues
} In a microstructurally uniform alloy, can one expect to get the chemical
} composition of the alloy through microprobe (assuming all the corrections
} are applied correctly)? Would the composition tally with the one
} determined by chemical analysis?
} TIA
} Anita



From daemon Sat Mar 04 09:11:38 2000



From: Purdy, Sam :      SPurdy-at-nationalsteel.com
Date: Fri, 3 Mar 2000 17:49:53 -0600
Subject: RE: Question Re: REM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In reply to David Bell's inquiry, I know some Eujropeans refer to an SEM as
a Raster Electron Microscope.

Sam Purdy
National Steel Technical Center
Trenton MI


} ----------
} From: "David_Bell-at-Millipore.com"-at-sparc5.microscopy.com
} Sent: March 2000 11:02 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Question Re: REM
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hello Colleagues,
}
} Twice now, I have had customers in Europe refer to a technique as REM.
} The
} sample prep sounds the same as SEM, and the first time I passed it off as
} a
} typo. Now, with this second reference, I'm not so certain. Is it
} possible
} that these customers are referring to SEM, possibly with their native
} language? Or is it a whole different technique/instrument? Could someone
} please help me clear up this confusion?
}
} Thanks in advance,
}
} David Bell
} Scientist
} Electron Microscopy Lab
} Millipore Corporation
} 80 Ashby Road
} Bedford, MA 01730
} (781) 533-2108
}
}




From daemon Sat Mar 04 09:11:38 2000



From: Purdy, Sam :      SPurdy-at-nationalsteel.com
Date: Fri, 3 Mar 2000 17:49:37 -0600
Subject: RE: hand sectioning - carbon blades

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I have in my hot little hand a box of carbon steel single edge razor blades
from Ted Pella. For the few times that I needed to to make a transparent
section (being a LM metallographer), I found these razor blades to be
effective.
Usual disclaimers

Sam Purdy
} ----------
} From: Grazyna M Tokarczyk
} Sent: March 2000 926  
} To: Gordon Couger
} Cc: c.jeffree-at-ed.ac.uk; Dan Luchtel; microscopy-at-sparc5.microscopy.com
} Subject: Re: hand sectioning - carbon blades
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
}
} On Thu, 2 Mar 2000, Gordon Couger wrote:
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} } Yes, carbon blades are still made - one of the makers is a Japanese
} company - I have a box in my hand, but unfortunately I can't read Japanese
} - the only English words on it is "Feather". There may also be many other
} makers of these in Asia and Eastern Europe.
}
} Grazyna Tokarczyk
} gmtokarc-at-is.dal.ca
} Dalhousie University
} Halifax, Canada
} }
} } ----- Original Message -----
} } } From: "Chris Jeffree" {cjeffree-at-srv0.bio.ed.ac.uk}
} } } By the way, the best type of blade for hand sectioning used to be
} } } the Blue Gillette double-edged carbon steel razor blades, which
} } } many of you may be too young to know about. The new, doubtless
} } } improved, stainless steel type never seems to be so sharp, and
} } } their edges dull faster. Is there still a manufacturer of carbon steel
} } } razor blades left in the world, or am I doomed to mourn forever?
} }
} } I miss the old blades as well. I cured the shaving problem I quit.
} } For hand sections I have better luck with a more rigid blade.
} } Razor blades and straight razors tend to dig in for me. I have
} } a lot better luck with a 2.5 inch wood chisel sharpened on glass
} } with silicone carbide grit. It is a lot of trouble to get sharp but
} } it works better for me than razor blades.
} }
} } Gordon
} }
} } Gordon Couger gcouger-at-couger.com
} }
} } Stillwater, OK www.couger.com/gcouger
} } 405 624-2855 GMT -6:00
} }
} }
} }
} }
}
}




From daemon Sat Mar 04 09:11:42 2000



From: MICROFAB-at-aol.com
Date: Fri, 3 Mar 2000 19:39:15 EST
Subject: LM - McArthur Blood Cell Counter

Contents Retrieved from Microscopy Listserver Archives
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I am looking for information on an instrument that I recently acquired. The
item is a McArthur Blood Cell Counter, which is a McArthur portable
microscope with a number of mechanical additions and a special stage,
apparently for doing blood count. It is in a neatly bundled case, very
portable, but lacking instructions. If anyone one is familiar with the
specific application of the set I would appreciate comments, particularly if
you have ever used such an instrument.

Jim Harper
microfab-at-aol.com



From daemon Sat Mar 04 09:11:44 2000



From: Mark YEADON :      m-yeadon-at-imre.org.sg
Date: Sat, 4 Mar 2000 09:15:46 +0800
Subject: Re: Question Re: REM

Contents Retrieved from Microscopy Listserver Archives
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There's also the technique of Reflection Electron Microscopy, which is the
imaging analogue of RHEED (reflection high energy electron diffraction). I
am sure your reference is to the SEM though, as the other respondents
indicate.

Just for interest, REM (like RHEED) is a surface sensitive technique and can
give beautiful images of monolayer surface steps, eg on the sapphire
surface, and this can all be done in the TEM. The sample is simply rotated
90 degrees from the usual TEM imaging position and so electrons are
'reflected' from the surface at glancing angle as they pass through the
sample chamber.

My best wishes to all,

Mark

%%%%%%%%%%%%%%%%%%%%%%%%%%%
Mark Yeadon
Senior Research Fellow
Institute of Materials Research and Engineering
3 Research Link
Singapore 117602

Assistant Professor
Department of Materials Science
National University of Singapore
Singapore 119602

TEL: (65) 874 8591
FAX: (65) 872 0785
email: m-yeadon-at-imre.org.sg


-----Original Message-----
} From: "David_Bell-at-Millipore.com"-at-sparc5.microscopy.com
To: "David_Bel-at-Millipore.com"-at-ultra5.microscopy.com
Cc: Microscopy-at-sparc5.microscopy.com
Sent: 3/4/00 3:20 AM



Hi all,

Thanks for a quick and consice response!!

David



From daemon Sun Mar 05 10:29:46 2000



From: jim :      jim-at-proscitech.com.au
Date: Sun, 5 Mar 2000 23:49:38 +1000
Subject: RE: Question Re: REM, History Note

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The original question has been answered well. However, I would like to add the
historical footnote that von Ardenne's seminal 1938 paper was entitled "Das
Elektronen-Rastermicroskop". A raster is a pattern of horizontal lines. The
German word is derived form the Latin "rake". I guess a rake's pattern is a
raster, a bit coarse for an SEM though.
Cheers
Jim Darley

ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Saturday, March 04, 2000 2:03 AM,
"David_Bell-at-Millipore.com"-at-sparc5.microscopy.com
[SMTP:"David_Bell-at-Millipore.com"-at-sparc5.microscopy.com] wrote:
}
}
} Hello Colleagues,
}
} Twice now, I have had customers in Europe refer to a technique as REM. The
} sample prep sounds the same as SEM, and the first time I passed it off as a
} typo. Now, with this second reference, I'm not so certain. Is it possible
} that these customers are referring to SEM, possibly with their native
} language? Or is it a whole different technique/instrument? Could someone
} please help me clear up this confusion?
}
} Thanks in advance,
}
} David Bell
} Scientist
} Electron Microscopy Lab
} Millipore Corporation
} 80 Ashby Road
} Bedford, MA 01730
} (781) 533-2108
}



From daemon Mon Mar 06 08:12:12 2000



From: Barbara Foster :      mme-at-map.com
Date: Sun, 05 Mar 2000 14:09:06 -0500
Subject: Re: Help:Naturpaths using Dark Field Microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Paul,

I have recently encountered several mentions of this type of thing
recently, in the oddest places. One was a presentation by the motivational
speaker, Tony Robbins, who, in front of 13,000 people at the Hartford Civic
Center, described how his recent feelings of fatigue and lack of energy had
been diagnosed by darkfield microscopy as due to chlamydia in his blood.
He cited work done by Dr. Robert Young at InnerLight.

As for crystal testing, I am still a strong supporter of Polarized light
work for that. (Interestingly, it looks a lot like darkfield and, perhaps,
is mis-cited by Naturpaths who might not know the difference).

Hope this is helpful.

Caveat: MME has no connection with either Mr. Robbins, Dr. Young, or
InnerLight.

Best regards,
Barbara Foster
Consortium President
Microscopy/Microscopy Education ...Educating microscopists for greater
productivity.

125 Paridon Street Suite 102 Springfield, MA 01118
PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com
Visit our web site {http://www.MME-Microscopy.com/education}
******************************************************



At 06:33 PM 3/1/00 -0600, pfieber-at-elite.net"-at-sparc5.microscopy.com wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Mon Mar 06 08:12:13 2000



From: Barbara Foster :      mme-at-map.com
Date: Sun, 05 Mar 2000 14:28:03 -0500
Subject: Re: SEM of DNA array

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Corazon,

We did a bit of market and applications research into this area for the 3
years before it emerged (as well as launching a company). These
microarrays are typically analyzed on larger scale using instruments which
are derivatives of confocal. If you are looking for individual DNA
molecules first make sure that they are really likely to be there (i. e,
get a full history of how this microarray was prepared and later used). I
would then suggest looking at it with AFM. ThermoMicroscopes, Digital,
and Molecular Imaging have all had experience in this area. Let me know if
you need specific contact info.

Best regards
Barbara Foster
Consortium President
Microscopy/Microscopy Education ...Educating microscopists for greater
productivity.

125 Paridon Street Suite 102 Springfield, MA 01118
PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com
Visit our web site {http://www.MME-Microscopy.com/education}


At 01:19 PM 3/2/00 -0600, Corazon Bucana wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Mon Mar 06 08:12:13 2000



From: Barbara Foster :      mme-at-map.com
Date: Sun, 05 Mar 2000 14:44:15 -0500
Subject: Re: LM - McArthur Blood Cell Counter

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Jim,

Not only have I had my hands on several McArthurs, I had the privilege of
visiting the inventor at home. Your comment about the "neat bundle" is
very accurate. Dr. McArthur showed me a number of accessories (including
fluorescence!) for this system. Although I did not have a chance for an
extended use, my recollection was that all of them worked passably well.

There were several generations of McArthur microscopes. The early ones
were made of metal and were very durable and usable. A somewhat later
generation was made of white plastic, I think for the BBC Open University.
The general opinion of some of my colleagues who knew this system indicated
that the earlier generation was a better bet.

I was not aware that anyone was commercially making this microscope today.
Dr. McArthur was advanced in years when I visited him over a decade ago
and, at that time, their construction was very much a cottage industry. Is
this a new system or are you buying it second hand? (I suspect the latter,
since you mentioned that the instructions are missing). It is hard to
comment on the bits and pieces without further information. I am planning
to attend a number of meetings (Experimental Bio, M&M, Cell Bio, Neuro as
well as Semicon West, Materials Solutions, and ISTFA) over the next 6
months. If you are going to any of them or will be in the area, I would be
delighted to take a look at what you have and see if I can dredge up any
old memories of how it works.

Finally, if you can send pictures, I can forward them to colleagues in the
UK who own McArthurs.

Hope this is helpful.

Best regards,
Barbara Foster
Consortium President
Microscopy/Microscopy Education ...Educating microscopists for greater
productivity.

125 Paridon Street Suite 102 Springfield, MA 01118
PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com
Visit our web site {http://www.MME-Microscopy.com/education}

At 07:39 PM 3/3/00 EST, MICROFAB-at-aol.com"-at-sparc5.microscopy.com wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Mon Mar 06 08:12:15 2000



From: Barbara Foster :      mme-at-map.com
Date: Sun, 05 Mar 2000 14:48:10 -0500
Subject: Re: Help:Naturpaths using Dark Field Microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Paul,

I have recently encountered several mentions of this type of thing
recently, in the oddest places. One was a presentation by the motivational
speaker, Tony Robbins, who, in front of 13,000 people at the Hartford Civic
Center, described how his recent feelings of fatigue and lack of energy had
been diagnosed by darkfield microscopy as due to chlamydia in his blood.
He cited work done by Dr. Robert Young at InnerLight.

As for crystal testing, I am still a strong supporter of Polarized light
work for that. (Interestingly, it looks a lot like darkfield and, perhaps,
is mis-cited by Naturpaths who might not know the difference).

Hope this is helpful.

Caveat: MME has no connection with either Mr. Robbins, Dr. Young, or
InnerLight.

Best regards,
Barbara Foster
Consortium President
Microscopy/Microscopy Education ...Educating microscopists for greater
productivity.

125 Paridon Street Suite 102 Springfield, MA 01118
PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com
Visit our web site {http://www.MME-Microscopy.com/education}
******************************************************



At 06:33 PM 3/1/00 -0600, pfieber-at-elite.net"-at-sparc5.microscopy.com wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

[message truncated]




From daemon Mon Mar 06 08:12:19 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Sun, 05 Mar 2000 16:03:41 -0600
Subject: Re: Help:Naturpaths using Dark Field Microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Amazing. Definitely a major feat of dark field light microscopy.
To be able to see and distinguish an organism that is between
0.1u and 0.5u is truly amazing.

Did he say what magnification was realized in this DF system?
The only DF condensers I know of for compound LMs are
not aplanatic. So is begs the question of how one could
resolve, much less see, such a tiny bacteria using LM.

Perhaps the sample was specially treated?

gary g.



At 01:09 PM 3/5/00 , you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Mon Mar 06 08:12:31 2000



From: Gerhard Frank :      frank-at-ww.uni-erlangen.de
Date: Mon, 06 Mar 2000 10:05:06 +0100
Subject: Re: Question Re: REM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


REM = 'Raster Elektronen Mikroskopie' in German (SEM in english) but
also
Reflection Electron Microscopy, which also needs not a lot of specimen
preparation. It looks very much like preparation for SEM in deed.
Regards
Gerhard Frank

"David_Bell-at-Millipore.com"-at-sparc5.microscopy.com wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hello Colleagues,
}
} Twice now, I have had customers in Europe refer to a technique as REM. The
} sample prep sounds the same as SEM, and the first time I passed it off as a
} typo. Now, with this second reference, I'm not so certain. Is it possible
} that these customers are referring to SEM, possibly with their native
} language? Or is it a whole different technique/instrument? Could someone
} please help me clear up this confusion?
}
} Thanks in advance,
}
} David Bell
} Scientist
} Electron Microscopy Lab
} Millipore Corporation
} 80 Ashby Road
} Bedford, MA 01730
} (781) 533-2108

--
Gerhard Frank
UNIVERSITAET ERLANGEN-NUERNBERG Phone: +49 9131 85 28606
Institut fuer Werkstoffwissenschaften VII Fax: +49 9131 85 28602
Cauerstr. 6, D-91058 Erlangen, Germany frank-at-ww.uni.erlangen.de



From daemon Mon Mar 06 08:12:37 2000



From: Barbara Foster :      mme-at-map.com
Date: Mon, 06 Mar 2000 08:32:11 -0500
Subject: Re: Help:Naturpaths using Dark Field Microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Gary,

Just a reminder that darkfield is detection limited, not resolution
limited. It only takes 2-3 photons, scattered from a feature, to indicate
that it is there.

Chlamydia is must be much bigger than the range you specified. We are in
the process of sending out a special mailer for a new spectral imaging
system, with chlamydia as the "cover shot". I think that this image was
acquired with a 40x objective, but will check. Data is intentionally not
provided on the card because we are encouraging recipients to go to the web
page.

Best regards,
Barbara Foster
Consortium President
Microscopy/Microscopy Education ...Educating microscopists for greater
productivity.

125 Paridon Street Suite 102 Springfield, MA 01118
PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com
Visit our web site {http://www.MME-Microscopy.com/education}


At 04:03 PM 3/5/00 -0600, Dr. Gary Gaugler wrote:
} Amazing. Definitely a major feat of dark field light microscopy.
} To be able to see and distinguish an organism that is between
} 0.1u and 0.5u is truly amazing.
}
} Did he say what magnification was realized in this DF system?
} The only DF condensers I know of for compound LMs are
} not aplanatic. So is begs the question of how one could
} resolve, much less see, such a tiny bacteria using LM.
}
} Perhaps the sample was specially treated?
}
} gary g.
}
}
}
} At 01:09 PM 3/5/00 , you wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Mon Mar 06 08:52:19 2000



From: Tobias Baskin :      BaskinT-at-missouri.edu
Date: Mon, 6 Mar 2000 08:32:43 -0600
Subject: Re: Help:Naturpaths using Dark Field Microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greetings,
To respond to a comment made by Gary Gaugler, and without in
ANY WAY seeking to validate or support the claims of naturopaths,
darkfield microscopy has imaged individual microtubules, diam 25 nm.
Same with Nomarski optics, and others. How is that possible? It is
important to keep in mind what the famous "resolution limit" means,
it means telling the difference between two and one object. It has
nothing to say about the smallest absolute size of an object that can
be detected.

If you imagine a perfectly black slide with a pinhole in it
made by a perfect iris that can shrink right down to infinitely small
(I did say a perfect iris), there is no limit in theory to how small
that pinhole can be and still be imaged. Once the diameter of the
pinhole becomes less than a certain length, set by diffraction, then
the size of the image of the pinhole will no longer get any smaller.
Instead, the contrast in the image will go down. But if you can put
enough light through and have a good enough detector, you can see
that image.

Going back to our microtubules, take a photo of the darkfield
image and measure the diameter of the microtuble in the image: it
will be around 250 nm or thereabouts.

For the record, the person who I think has published the most
dark field images of microtubules is called Hotani, he works in
Japan, I am not sure where.

Of course, doing this in practice is not easy, but it is
certainly possible.

As ever,
Tobias


} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_ ____ ^ __ ____ Tobias I. Baskin
/ \ / / \ / \ \ University of Missouri
/ | / / \ \ \ Biological Sciences
/___/ /__ /___ \ \ \__ 109 Tucker Hall
/ / / \ \ \ Columbia, MO 65211-7400 USA
/ / / \ \ \ voice: 573-882-0173
/ /____ / \ \__/ \____ fax: 573-882-0123



From daemon Mon Mar 06 10:02:32 2000



From: DrJohnRuss-at-aol.com
Date: Mon, 6 Mar 2000 10:42:54 EST
Subject: 16 bit image processsing and measurement plugins for Photoshop

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


An earlier thread on this listserver (May-June 1999, and also summarized in
the January/February 2000 issue of Microscopy and Microanalysis) asked about
the availability of software for the processing and analysis of 16 bit per
channel images, which are obtained from flat bed scanners, cooled digital
cameras, and some microscopes (especially AFMs). Following the encouragement
of members of this list, we've written a set of image processing and
measurement routines for these images. Those interested can get information
at http://members.aol.com/FoveaPro/

Disclaimer: Please note that Fovea Pro is a commercial product sold by
Reindeer Games. My connection with the company is through my son, Chris, who
wrote the programs, and my own role in writing the accompanying tutorial.

John Russ



From daemon Mon Mar 06 10:12:31 2000



From: Christine Brantner :      brantner-at-codon.nih.gov
Date: Mon, 6 Mar 2000 11:15:10 -0400
Subject: Tracor Northern 5500

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greetings

We have a Tracor Northern 5500 EDX unit that we would like to give away.
It comes with a manual, mainframe, and monitor. If anyone is interested
please contact:

Dr Brian Andrews
NIH, NINDS
Bethesda MD
301-435-2796

sba-at-helix.nih.gov


Thanks
Chris
:):):):):):):):):):):):):):):):):):):):):):):):):):):):):):)

Christine A. Brantner, Ph.D.

Biologist/Lab Manager

National Institutes of Health
National Institute of Neurological Diseases and Stroke
Lab of Neurobiology
Section of Analytical Cell Biology
Bldg 36, room 2A21 phone 301-435-2803
36 Convent Dr fax 301-480-1485
Bethesda, MD 20892-4062



From daemon Mon Mar 06 10:42:21 2000



From: Nina Allen :      nina_allen-at-ncsu.edu
Date: Mon, 6 Mar 2000 11:39:31 -0500
Subject: Re: Help:Naturpaths using Dark Field Microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello:
I will add just a few comments to those of Tobias Baskin. When we
first developed Video Microscopy in the early eighties, we were visulizing
microtubules and other structures smaller than the ca. 200 nm resolution
limit using DIC and computer image enhancement. This is discussed in the
early papers from 1981 and 1983 (R.D. Allen and N.S. Allen 1983, J. of
Microscopy),and more easily available in the Inoue and Spring second
edition of Video Microscopy, Plenum Press. The important thing to remember
is that you can visualize items smaller than 100 nm, but you are not
resolving them.
With the new method Polarization Modulation DIC we get very good images of
microtubules and other fine structures (Holzwarth, G., Webb, S.J.,
Kubinski, D.J., N.S. Allen. 1997. Improving DIC Microscopy with
Polarization Modulation. Jour. of Microscopy 188:249-254). Contrast is
doubled with this method and it is worth a try. It is commercially
available from Hamamatsu or you can build your own ,Nina Allen
} Greetings,
} To respond to a comment made by Gary Gaugler, and without in
} ANY WAY seeking to validate or support the claims of naturopaths,
} darkfield microscopy has imaged individual microtubules, diam 25 nm.
} Same with Nomarski optics, and others. How is that possible? It is
} important to keep in mind what the famous "resolution limit" means,
} it means telling the difference between two and one object. It has
} nothing to say about the smallest absolute size of an object that can
} be detected.
}
} If you imagine a perfectly black slide with a pinhole in it
} made by a perfect iris that can shrink right down to infinitely small
} (I did say a perfect iris), there is no limit in theory to how small
} that pinhole can be and still be imaged. Once the diameter of the
} pinhole becomes less than a certain length, set by diffraction, then
} the size of the image of the pinhole will no longer get any smaller.
} Instead, the contrast in the image will go down. But if you can put
} enough light through and have a good enough detector, you can see
} that image.
}
} Going back to our microtubules, take a photo of the darkfield
} image and measure the diameter of the microtuble in the image: it
} will be around 250 nm or thereabouts.
}
} For the record, the person who I think has published the most
} dark field images of microtubules is called Hotani, he works in
} Japan, I am not sure where.
}
} Of course, doing this in practice is not easy, but it is
} certainly possible.
}
} As ever,
} Tobias
}
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America


-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-
Nina Stromgren Allen
Professor, Department of Botany;
Director, Cellular and Molecular Imaging Facility
Box 7612, Department of Botany
North Carolina State University
Raleigh, NC 27695-7612
Phone: 919-515-8382 (Office), 515-3525 (Lab), 515-2727 (Department Secretary)
Fax: 919-515-3436





From daemon Mon Mar 06 11:32:14 2000



From: Baliga, Vicki L. :      Vicki.L.Baliga-at-pmusa.com
Date: Mon, 6 Mar 2000 12:20:30 -0500
Subject: Need a High Resolution Printer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Is anyone familiar with a printer(s) that can provide equivalent
detail to a polaroid print or to the images that can be collected from the
scanning electron microscopes? I have looked at QMS Color Magic II printers
that have 2400 dpi x 600 dpi, 257 shades of gray, with a cell size of 150
cells per inch (lines per inch). This is comparable to a Codonics 300 line
dye-sub printer that I have seen. However, the dye-sub printers tend to
smear fine details to the point of not being visible. I saw an add for an
Alps printer with a dot size of 10 microns at 2400 dpi. The add did not
indicate the shades of gray so I have no way of knowing what the numer of
cells per inch are. I suspect that it would have 257 shades of gray which
would give a cell size of 16x16 and a cells or lines per inch of 150. Does
anyone have user information about these or any other printers that might
work?
No one talks about the cells per inch (lines per inch) in any of the
documentation. For me, cells per inch has been the best measure of
resolution. For the printers that we have, the extra dots (300 dpi matrix
4x4, 600 dpi matrix 8x8, 1200 dpi matrix 12x12, 2400 dpi matrix 16x16) serve
to provide more dynamic shades of gray. A 300 dpi with 17 shades of gray
and a 600 dpi with 65 shades of gray both have 75 cells per inch. The only
difference is more contrast in the 600 dpi with 65 shades of gray. A simple
formula for this is dpi/cell matrix size= cells (lines) per inch, 2400
dpi/16=150 cells per inch and a maximum # of shades of gray =
1+(dpi/lpi)squared. This tid bit of information came from The Image
Processing Handbook, 2nd ed. by John C. Russ.
I would appreciated any help that I can get in finding a printer
that will do the job. Thanks.

Vicki Baliga
Vicki.L.Baliga-at-pmusa.com
804-274-3088



From daemon Mon Mar 06 12:22:06 2000



From: DrJohnRuss-at-aol.com
Date: Mon, 6 Mar 2000 13:03:10 EST
Subject: Re: Need a High Resolution Printer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



In a message dated 3/6/00 5:55:31 PM, Vicki.L.Baliga-at-pmusa.com writes:

.."A 300 dpi with 17 shades of gray
and a 600 dpi with 65 shades of gray both have 75 cells per inch. The only
difference is more contrast in the 600 dpi with 65 shades of gray. A simple
formula for this is dpi/cell matrix size= cells (lines) per inch, 2400
dpi/16=150 cells per inch and a maximum # of shades of gray =
1+(dpi/lpi)squared. This tid bit of information came from The Image
Processing Handbook, 2nd ed. by John C. Russ...."

I guess it is worth pointing out that the calculation Vicki has quoted here
is a bit optimistic. Most printers have dots that overlap somewhat, and the
dots may spread a bit on the paper. Plus the dots are large enough to be
resolved by the human eye, which creates interesting effects when they start
to touch or overlap. And the shades of grey that perfect dots might produce
would be linear rather than log, as human vision perceives brightness. It
would be conservative to estimate that a printer that seems technically
capable of producing (e.g.) 65 shades of grey in a given printing cell size
will actually produce images with about half that number of really useful
shades of grey. But that is still OK since that is about all the distinct
shades that a person can detect on a print (and more than most Polaroid
prints have, although the negatives are much better).

Halftone printing is never going to be as good as something that really
covers the resolution cell with a uniform shade of grey (or color). Dye sub
and a few ink jet printers do a much better job than laser printers in that
regard.

John Russ



From daemon Mon Mar 06 14:01:52 2000



From: Catherine Ripley :      ripley-at-aecom.yu.edu
Date: Mon, 06 Mar 2000 14:48:13 -0500
Subject: Confocal Newslist or Listserver??

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Larry

I sent an email to LISTSERV-at-LISTSERV.ACSU.BUFFALO.EDU as you suggested with
SUBSCRIBE CONFOCAL in the body of the message.

I got this message back:
No LISTSERV list by the name of "CONFOCAL," is known to exist. Note that
lists can be marked "confidential" and that the existence of such lists is
usually known only to the server that is actually hosting it.

I'm very interested in finding a venue that is more oriented toward
confocal microscopy. Any further suggestions?




Catherine Ripley
*****************************************
Albert Einstein College of Medicine
1300 Morris Park Avenue
Bronx, NY 10461
*****************************************
*****************************************



From daemon Mon Mar 06 18:11:17 2000



From: Mel Dickson :      m.dickson-at-unsw.edu.au
Date: Tue, 07 Mar 2000 10:55:26 +1100
Subject: Microscopy of blood

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Microscopists can look at their own blood!

If you take a drop of blood from a pinprick and put it on a slide, you will
see (at rather lower magnification) what the "live cell" analysts purport
to see. You will see red cells starting to agglutinate. Thats all. It is
nearly impossible to find a white cell. If you find a bacterium you are
probably near death. Using dark field adds mystery but no more useful
information.

Check out these websites for three different perspectives on live cell
microscopy


1. http://www.hcrc.org/faqs/livecell.html

Critical evaluation pointing out the supposed features naturopaths claim as
showing pathological change are natural variation.



2. http://www.veg.on.ca/lifelines/janfeb/analysis.htm

A midly critical warning that most Live Cell Analysis practitioners are not
professional pathologists!

3. http://www.tlpdesign.com/hplusw/alt_directory/livecellanalysis.html

Gushing plug for the technique including claiming ability to detect poor
nutrition, vitamin deficiency etc. See the extract below.


None of these claims that illness can be diagnosed by observing
coverslipped blood have any scientific validity at all.

"Live cell analysis can determine cellular nutritional status. Nutritional
status is essential to aid in curbing and reversing the free radical
cascade of destructive, deteriorating cell structures.

This unique analysis of peripheral blood can reveal a good prospectus of
the immune system. By observing the morphology of the white corpuscles and
their activity in contrast to the extent of active foreign antigens and
microbes within the serum and red blood cells, the strength of the immune
system
can be judged. Weakness and dysfunction in any one of these three major
components and influence the strength and function of the other two. This
can be indicative of a progressive disease process."



From daemon Mon Mar 06 18:31:17 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 06 Mar 2000 16:06:36 -0600
Subject: Re: Need a High Resolution Printer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


A photographic negative and/or print is continuous tone. Digital
printers (ink jet, laser, etc.) attempt to reproduce continuous tone
via modulation of the size of the dots or the number of dots in
an area. Continuous tone digital printers typically use dye sublimation
to produce photographic quality results. In this regard, there really
isn't a true correspondence to dpi. Alternatively, some ink jets use
CMYK or even 5 color jets rather than RGB or RGB+B. Some of the
new generation do a rather good job of color printing.

But since you are talking about SEM images, these are gray scale
images rather than color. So, what are the options for b/w?

600dpi and 1200dpi laser printers do a rather good job of printing
SEM images. One must adjust the toner density to achieve maximum
quality results. But regardless, they are not continuous tone prints.
The closest thing to continuous tone b/w is the Orion (I think that
is the name) printer. It is an effective 300dpi and prints on expensive
paper--which is par for the course, considering the cost of the
printer (about $25K). And the printer is sloooooow.

I use a HP Laserjet 4M Plus and am very pleased with the results.
But the printed images are not archival and are not continuous tone.
But they are very inexpensive.

If you can describe what your actual end purpose is, perhaps others
could offer some good options for you to consider. Are you talking
about proofing, archiving, presentations, etc?

gary g.



At 11:20 AM 3/6/00 , you wrote:
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From daemon Mon Mar 06 22:20:18 2000



From: JUPE Peter :      JUPE.Peter-at-hdh.com.au
Date: Tue, 7 Mar 2000 14:35:46 +1100
Subject: Transmission electron microsocopy

Contents Retrieved from Microscopy Listserver Archives
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Dear Sir
Could you please supply me details on "transmission electron microsocopy"
for my 16yr old daughter at high school, or alternatively where to look on
the Internet.
Thanking you
Peter Jupe



From daemon Tue Mar 07 08:06:11 2000



From: Ken Tiekotter :      tiekotte-at-up.edu
Date: Tue, 7 Mar 2000 01:29:48 -0800 (PST)
Subject: Splitting hairs or Chylamdiae

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Splitting hairs on detecting microtubules vs. detecting pathogenic
organisms...

I guess I will have a go at this and muddy the waters...

As a background...The inability of Chlamydiae to produce metabolic energy
restricts these obligate 'parasites' to an intracellular existence.

The infectious component (elementary body) is about 3um in diameter, well
within the resolvable range of the OLM. Once acquired in the host cell
through phagocytosis, a vaculated 'initial body' (0.5 -1.0um) is formed:
huge by OLM standards!

'Elementary bodies' and 'initial bodies' possess distinctive staining
properties using Giemsa staining and Macchiavello's staining techniques,
not be mention detection by immunocytochemistry.

One can perhaps differentiate Chlamydia induced inclusion bodies
juxtaposed to the nucleus of the host cell using Darkfield illumination.
However, differential diagnosis of Chylamydia by Darkfield alone is
questionable.

If one considers Darkfield and the ability of a 'substance' of a given
refractive index to scatter light with respect to the R.I. of the adjacent
material, we must accept the notion of "detection vs. resolution' (B.
Foster ' Optimizing Light Microscopy for Biological and Clinical
Laboratories', pg. 57): therefore, we may detect an inclusion body, but
can we identify the pathogen of the inclusion body? Perhaps Phase
Contrast would be more sensitive to refractive differences?

As the devil's advocate, I see the issue of using Darkfield as a definite
diagnostic tool being dilute to 'mine is smaller than yours'. Not being
up on the naturopathic literature, I question how only using Darkfield
Illumination can definitely dx Chlamydia?

However, comparing various organisms and the use of Darkfield
Illumination, 'Treponema pallium' can easily be detected by Darkfield; an
organism of 0.2 um wide, but 5-15 um in length! Furthermore, the
inclusion body of Rabies can easily be detected by Darkfield and
Brightfield; however, we i.d. the total sum of virons: an example of
'detection vs. resolution'.

Yes, we as microscopists scoff at unconventional claims. Perhaps,
Mr. Tim Robbins should present a blood sample for us to examine under
the light of mutual cooperation and enlightenment'!!


I present my premise for your delicate evisceration...

Cheers!

-Ken






From daemon Tue Mar 07 08:06:15 2000



From: John Runions :      cjr41-at-cam.ac.uk
Date: Tue, 7 Mar 2000 11:35:10 +0100
Subject: Re: Confocal Newslist or Listserver??

Contents Retrieved from Microscopy Listserver Archives
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Catherine,

I also tried to subscribe to the confocal list when Larry suggested
it and it worked like a charm. I cut and pasted the email address
right from his posting.

LISTSERV-at-LISTSERV.ACSU.BUFFALO.EDU

Try again. Cheers, John


} ------------------------------------------------------------------------
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--
C. John Runions, Ph.D.
Department of Plant Sciences
University of Cambridge
Cambridge CB4 1YE
UK

email at work cjr41-at-cam.ac.uk
email at work runions-at-ntlworld.com
Phone (01223) 766 545



From daemon Tue Mar 07 08:06:17 2000



From: Cap'n Peachfuzz :      bsteffen-at-arches.uga.edu
Date: Tue, 7 Mar 2000 08:48:54 -0500 (Eastern Standard Time)
Subject: Re: Microscopy of blood

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In the earlier days {25 years ago} of medical diagnosis, before the
advent of the rapid, highly specific methods of identifying pathogenic
organisms, darkfield microscopy was routinely applied to rule out
specific organisms. In particular, the diagnosis of syphilis was aided
with this mode of imaging. When fluids from a suspect lesion were
applied to a slide and imaged by darkfield, the characteristic shape of
the spirochaete organisms was clearly seen. More recently, darkfield
examination of synovial fluids from arthritic joints was used to rule
out or diagnose Lyme disease, before specific testing became available.

W. L. Steffens, Ph.D
University of Georgia



From daemon Tue Mar 07 18:06:02 2000



From: rlvaughn-at-unmc.edu
Date: Tue, 7 Mar 2000 17:06:42 -0600
Subject: TEM - ImmunoEM reference

Contents Retrieved from Microscopy Listserver Archives
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Is there a short, general reference, like a review, that explains immuno
EM, it's uses, major techniques, and shortfalls? I would like to use it to
send to investigators that are thinking about using the technique but don't
know much about labeling at the EM level. I have a lot of specific
references and it would take a lot of time to reference or copy them. If
the investigator wants to know more I can direct them to those, otherwise a
general one would get them and me on the same page when we start talking
specifics on their project. Thanks

Rick Vaughn
rlvaughn-at-unmc.edu




From daemon Wed Mar 08 07:36:58 2000



From: Paul Webster :      pwebster-at-hei.org
Date: 08 Mar 00 00:40:11 -0800
Subject: TEM - ImmunoEM reference

Contents Retrieved from Microscopy Listserver Archives
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------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Rick Vaughn writes:
Is there a short, general reference, like a review, that explains immuno
EM, it's uses, major techniques, and shortfalls? I would like to use it to
send to investigators that are thinking about using the technique but don't
know much about labeling at the EM level. I have a lot of specific
references and it would take a lot of time to reference or copy them. If
the investigator wants to know more I can direct them to those, otherwise a
general one would get them and me on the same page when we start talking
specifics on their project. Thanks
Rick Vaughn
rlvaughn-at-unmc.edu

The best reference you can ask for is the 1993 book by Gareth Griffith: "Fine Structure Immunocytochemistry." Springer Verlag. In it you will find all the major techniques, pitfalls, antibody use, fixatives and even quantitative methods for estimating antigen number. My copy has been replaced many times, I think because someone else needed it more than I did!

Paul Webster.




From daemon Wed Mar 08 18:23:47 2000



From: Jaci Lett :      jmlett-at-cid.wustl.edu
Date: Wed, 8 Mar 2000 18:09:50 -0600
Subject: methods of embedding cells cultured on glass coverslips

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I know this has been covered periodically, but as I've not needed the
technique, I've not paid attention.  I need some methods of embedding
cells cultured on glass coverslips.  Obviously, the most important
issue is removing the coverslip from the polymerized block.I've looked
through my own reference materials and the only method I found involved
using Thermanox coverslips (upon which these particular cells will not
grow, I'm informed).Please reply dircectly to my email address as well as
to the Listserver (I haven't received postings for several days). Thank
you,Jaclynn M. Lett, Research Assistant    
jmlett-at-cid.wustl.eduFay and Carl Simons Center for Biology of Hearing and
DeafnessCentral Institute for the Deaf4560 Clayton AvenueSt. Louis,
MO  63110voice:  314-977-0257     fax: 
314-977-0030




From daemon Wed Mar 08 18:23:47 2000



From: R schlicher :      rkslick-at-yahoo.com
Date: Wed, 8 Mar 2000 18:14:50 -0600
Subject: Rapid Fixation of cells for SEM)

Contents Retrieved from Microscopy Listserver Archives
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Hello,

I need to fix cells for viewing with SEM. However,
the fixation method needs to be consistent with my
experimental methods:

1) I need to fix the cells in less than 1 minute and
due to this time limitation, I cannot spin the cells
into a pellet and must add the cell suspension to the
fixative; and

2)I am looking for "effects" to the cell membrane
caused by my experiment, so I need to keep any
possible fixative damage/effects at a minimum.

I have fixed cells using plunge freezing and viewed
them with TEM, this is time consuming and I ran into
a
lot of freeze damage. I would like to try chemical
fixation and SEM. It has been suggested that I try
glutaraldehyde + buffers, then postfix with osmium
tetroxide and dehydrate in an ethanol series.

Is this the best method? I am open to suggestions.
I am using suspensions of prostate cancer cells in
RPMI (+serum) growth media; I can perform the
experiment in buffered saline and use a high
concentration of cells.

Thanks,
Robyn


Robyn K. Schlicher, M.S.
Laboratory for Drug Delivery
Institute for Bioengineering and Biosciences
Georgia Institute of Technology
315 Ferst Drive
Atlanta, GA 30332
rkslick-at-yahoo.com


__________________________________________________
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Talk to your friends online with Yahoo! Messenger.
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From daemon Wed Mar 08 18:23:47 2000



From: amit kaulgud :      amit_kaulgud-at-hotmail.com
Date: Wed, 8 Mar 2000 18:11:39 -0600
Subject: OsO4 with PTFE

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



hi
} i am doing research at university of massachusetts lowell.i am working on
} charaterization of Polytetrafluoroethylene(PTFE).While doing TEM i have
} stained the PTFE with osmium tetroxide.Can any one enlighten me on the
} mechanism oF OsO4 with PTFE?
} thanks
} ________________________________________________
} Amit A.Kaulgud
} Research Assistant.
} University of Massachusetts Lowell
} Department of Chemical/Materials Engineering.
} 170, Riverside Street,#3
} Lowell MA -01854.
} Phone/Voice:978-459-3248.
} email: amit_kaulgud-at-hotmail.com

______________________________________________________
Get Your Private, Free Email at http://www.hotmail.com




From daemon Wed Mar 08 18:23:48 2000



From: Schmitz, Robert :      rschmitz-at-uwsp.edu
Date: Wed, 8 Mar 2000 18:09:05 -0600
Subject: 2.5% glut shelflife

Contents Retrieved from Microscopy Listserver Archives
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Below is a question from one of my former students who is now working in a
hopsital histotech lab. It concern the selflife of glutaraldehyde fixative.
I always mix my fix fresh, so some other perspectives might be useful!!
Here's the Question:

} My boss wants to know how long a working solution of glutaraldehyde
} remains stable (2.5% in cacodyalate buffer). How long can we keep it in
} the refrigerator?
}
Bob
Robert J. Schmitz
Electron Microscope Lab
Department of Biology, CNR Building
University of Wisconsin, Stevens Point
Stevens Point, WI 54481
phone (715) 346-2420
FAX (715)346-3624
email rschmitz-at-uwsp.edu
http://biology.uwsp.edu/faculty/RSchmitz/home.html




From daemon Wed Mar 08 18:53:42 2000



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Wed, 08 Mar 2000 16:40:22 -0800
Subject: TEM - ImmunoEM reference

Contents Retrieved from Microscopy Listserver Archives
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In addition, I would point that Paul's web site at
http://www.hei.org/htm/cryo.htm is not bad source, too.

} Date: Wed, 08 Mar 2000 00:40:11 -0800
} From: Paul Webster {pwebster-at-hei.org}
} Subject: TEM - ImmunoEM reference
} To: MSA listserver submission {Microscopy-at-sparc5.microscopy.com}
} Reply-to: Paul Webster {pwebster-at-hei.org}
} X-Mailer: QuickMail Pro 1.5.4 (Mac)
} X-MIME-Autoconverted: from quoted-printable to 8bit by
ultra5.microscopy.com id
} CAA01345
}
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Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu
http://www.bol.ucla.edu/~sryazant




From daemon Wed Mar 08 20:37:20 2000



From: John F. Mansfield :      jfmjfm-at-engin.umich.edu
Date: Wed, 8 Mar 2000 20:28:33 -0600
Subject: ESEM Symposium at the Fall MRS

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At the Fall MRS this year there will be a symposium dedicated to
environmental SEM and low vacuum SEM, invited speakers include Eric
Doehne from Getty Conservation Institute, David Joy from the
University of Tennessee and Matthew Phillips from The University of
Technology in Sydney. Contributed papers from all environmental SEM
and low vacuum SEM users are encouraged, the Preliminary Call for
Papers follows:

Preliminary Call for Papers , Session for MRS 2000 (Boston)
http://www.mrs.org/meetings/fall2000/cfp/symposia.html

Low Vacuum SEM/ESEM in Materials Science
"Wet SEM: the Liquid Frontier of Microscopy"

Abstract Deadlines: June 5th for abstracts sent by mail and June 19th
for abstracts submitted via the MRS Web Site (http://www.mrs.org).

Scanning electron microscopy and x-ray microanalysis can now be
performed at pressures as high as 1 - 5000 Pa (~0.01 to 40 torr) in
the class of instruments known variously as "low vacuum", "elevated
pressure", and "environmental" SEMs. This represents an increase of
3 to 6 orders of magnitude in pressure compared to the operating
conditions of ordinary high vacuum SEMs. At the upper end of this
pressure range, water can be maintained in the liquid state at
temperatures from 3 - 10 deg. C. This remarkable situation has
opened up broad new areas of opportunity in many fields, especially
materials science. Dynamic processes can be observed with many of
the familiar strengths of conventional SEM: high resolution, large
depth of focus, and a variety of imaging signals that reveal
compositional, topographic, electrical and other specimen properties.
X-ray microanalysis for elemental characterization is also possible,
although somewhat compromised. Chemical, physical, and mechanical
experiments can readily be performed in user-specified environments.
The microscope can thus be thought of as an appendage to an
experimental chamber. Papers are solicited in all aspects of
materials science in which this new microscopy is used.

Co-organizers:

Dr. Brad Thiel
Polymers & Colloids Group
Cavendish Laboratory
University of Cambridge
Madingley Road
Cambridge, CB3OHE, UK
44 1223 337 272
44 1223 337 000 (fax)
bt202-at-cam.ac.uk

Dr. John Mansfield
North Campus Electron Microbeam Analysis Laboratory
417 SRB, University of Michigan
2455 Hayward, Ann Arbor MI 48109-2143
(734) 936-3352
(734) 763-2282 FAX
jfmjfm-at-engin.umich.edu

Dr. Dale Newbury
Surface and Microanalysis Science Division
National Institute of Standards and Technology
Gaithersburg, MD 20899-8371 USA
301-975-3921
301-417-1321 (fax)
dale.newbury-at-nist.gov


















--



Dr. John Mansfield CPhys MInstP
North Campus Electron Microbeam Analysis Laboratory
417 SRB, University of Michigan
2455 Hayward, Ann Arbor MI 48109-2143
Phone: (734) 936-3352 FAX (734) 763-2282 Cellular Phone: (734) 358-7555
(Leaving a phone message at 936-3352 is preferable to 358-7555)
Email: jfmjfm-at-engin.umich.edu
URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html
Location: Lat. 42#161# 16' 48" Long. 83#161# 43' 48"






From daemon Thu Mar 09 02:26:29 2000



From: Kyrill Ukhanov :      ukhanov-at-rz.uni-potsdam.de
Date: Thu, 9 Mar 2000 09:18:59 +0100 (MET)
Subject: fluoronanogold stability

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I have used fluoronanogold for a couple of times and it did not work on the
EM level. Then I realized that this batch has been already expired. Does
anyone know if upon aging fluoronanogold could lose gold leaving only FITC
molecule attached to Ig? I did see a fluorescence signal before running
silver enhancement though. Currently I will try regular ultrasmall gold
probe to clear up this problem but I wonder what was the trick.

thanks for your help

Kyrill

**********************
Dr. Kyrill Ukhanov
Institute for Zoophysiology, University of Potsdam,
Lennestrasse 7a, D-14471 Potsdam, Germany
phone +49-0331-9774859
fax +49-0331-9774861



From daemon Thu Mar 09 03:46:18 2000



From: Alexander Mironov :      mironov-at-cmns.mnegri.it
Date: Thu, 9 Mar 2000 10:40:13 +0100 (MET)
Subject: Re: methods of embedding cells cultured on glass coverslips

Contents Retrieved from Microscopy Listserver Archives
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Dear Jaci,
The problem of cell embedding after their cutivation on plastic or galss
is rather simple. You do not need to use Thermanox. You can use glass. The
trick is that immediately after polymerization you have place glasses at
-20 degree C and your samples will be detached. If thsi scheme does not
work you can use liquid nitrogen. However, do not insert smplaes into it
(only glass). If this scheme does not work you can use commercial solution
of HF (acid). It dissolve glass efficiently. For instance, coverslips are
dissolved in 30 min. Then you have to wash samples in buffer (pH7.4) for a
long time and water (several hours) otherwise you will have problems with
contrasters.
We usually glue samples after detachment from glass and work with
this sandwich. For this you have to use incomplete polymerization of Epon
on chamber slides (12 hours). Then samples can be detached at -20 and then
glued with the fresh resin for 24 hours. If you use full polymerization
time on glass or any polymerization on plastic you will not be able to
glue samples.

Sincerely yours, Alexander Mironov
Italy


On Wed, 8 Mar 2000, Jaci Lett wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I know this has been covered periodically, but as I've not needed the
} technique, I've not paid attention.  I need some methods of embedding
} cells cultured on glass coverslips.  Obviously, the most important
} issue is removing the coverslip from the polymerized block.I've looked
} through my own reference materials and the only method I found involved
} using Thermanox coverslips (upon which these particular cells will not
} grow, I'm informed).Please reply dircectly to my email address as well as
} to the Listserver (I haven't received postings for several days). Thank
} you,Jaclynn M. Lett, Research Assistant    
} jmlett-at-cid.wustl.eduFay and Carl Simons Center for Biology of Hearing and
} DeafnessCentral Institute for the Deaf4560 Clayton AvenueSt. Louis,
} MO  63110voice:  314-977-0257     fax: 
} 314-977-0030
}
}
}
}



From daemon Thu Mar 09 06:45:51 2000



From: jim :      jim-at-proscitech.com.au
Date: Thu, 9 Mar 2000 21:13:19 +1000
Subject: RE: methods of embedding cells cultured on glass coverslips

Contents Retrieved from Microscopy Listserver Archives
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A few moments in liquid nitrogen and the coverslip will come off.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Thursday, March 09, 2000 10:10 AM, Jaci Lett [SMTP:jmlett-at-cid.wustl.edu]
wrote:
}
}
} I know this has been covered periodically, but as I've not needed the
} technique, I've not paid attention.  I need some methods of embedding
} cells cultured on glass coverslips.  Obviously, the most important
} issue is removing the coverslip from the polymerized block.I've looked
} through my own reference materials and the only method I found involved
} using Thermanox coverslips (upon which these particular cells will not
} grow, I'm informed).Please reply dircectly to my email address as well as
} to the Listserver (I haven't received postings for several days). Thank
} you,Jaclynn M. Lett, Research Assistant    
} jmlett-at-cid.wustl.eduFay and Carl Simons Center for Biology of Hearing and
} DeafnessCentral Institute for the Deaf4560 Clayton AvenueSt. Louis,
} MO  63110voice:  314-977-0257     fax: 
} 314-977-0030
}
}



From daemon Thu Mar 09 06:45:51 2000



From: jim :      jim-at-proscitech.com.au
Date: Thu, 9 Mar 2000 22:00:42 +1000
Subject: RE: 2.5% glut shelflife

Contents Retrieved from Microscopy Listserver Archives
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I presume all microscopists use somewhat arbitrary shelf-life assumptions,
because we know from experience that within those constraints there normally is
no trouble. UA in water } 1 month, made up Spurr's in freezer } 3 months, Lead
Citrate } 1 year. For working strength GA I used quite conservatively 1 week.

GA forms an undesirable polymer with time and temperature. So freezing the bulk
stock (ampoules can stand a fridge-freezer) results in a very long life,
certainly a couple of years and just refrigerated its several months. This is
arbitrary too since there is no definite cut-off when the stuff suddenly
becomes useless.

I saw a publication many years ago that claimed the "undesirable polymer"
protected cells against osmotic shock and in structural studies some polymer
may be desirable (the amount can be determined simply in a spectrometer, please
don't ask for the peak locations. I don't carry that info!) Apparently its
different for immunological studies, for that the freshest GA should be used.

Soon after Sabatini advocated GA for TEM use, I guess now over 30 years ago, as
noted, some studies related polymer to storage time/temperature. I don't think
that these looked at working strength solutions, but I expect that there would
be no difference in relative polymer concentrations.

The reason for shorter shelf-life for working solutions was assumed and
probably originated with the common use of additives like sucrose or phosphate
buffers. Sucrose in an Os fixatives leads to obvious oxidation within hours. I
doubt that cacodylate in GA would be a problem and expect that it would remain
useable for several months when refrigerated. I have never bothered to test
this, however, and stuck with an assumed short shelf-life for working strengths
GA.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Thursday, March 09, 2000 10:09 AM, Schmitz, Robert [SMTP:rschmitz-at-uwsp.edu]
wrote:
}
}
} Below is a question from one of my former students who is now working in a
} hopsital histotech lab. It concern the selflife of glutaraldehyde fixative.
} I always mix my fix fresh, so some other perspectives might be useful!!
} Here's the Question:
}
} } My boss wants to know how long a working solution of glutaraldehyde
} } remains stable (2.5% in cacodyalate buffer). How long can we keep it in
} } the refrigerator?
} }
} Bob
} Robert J. Schmitz
} Electron Microscope Lab
} Department of Biology, CNR Building
} University of Wisconsin, Stevens Point
} Stevens Point, WI 54481
} phone (715) 346-2420
} FAX (715)346-3624
} email rschmitz-at-uwsp.edu
} http://biology.uwsp.edu/faculty/RSchmitz/home.html
}
}



From daemon Thu Mar 09 08:19:13 2000



From: puusimak-at-paju.oulu.fi
Date: Thu, 9 Mar 2000 08:06:45 -0600
Subject: fixation-method needed

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Email: puusimak-at-paju.oulu.fi
Name: Päivi Maria Susanna Uusimäki

School: the university of Oulu

Question: What is a suitable fixation-method for my
specimens? I am trying to find antigen-antibody
interactions on the bacterial membrane. My
bacteria are different strains of LABs, my special
interests are lipoteichoic acids and I am using
confocal and fluorescence microscopes. The big
problem has
been : specimens tend to "float away" under was-
hings !




From daemon Thu Mar 09 14:30:51 2000



From: Chris Adams :      cadams-at-lanl.gov
Date: Thu, 9 Mar 2000 08:19:26 -0700
Subject: TEM: Electron Tomography

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I have recently been asked to participate in an effort at Los Alamos
concerned with Electron Tomography of polymers. I haven't done the
required image reconstruction before. Does anyone know of any
commercially available software that will reconstruct several 2D
images taken at various tilt angles into a single 3D reconstruction
of an object? I suppose it would be analogous to the software used
for CAT scans? Also, if you've done this before, what kind of
hardware requirements are there? I've got a JEOL 2010 configured at
the moment to allow only ±30 degrees of tilt. Is that enough? I
assume I also need some way of automating the specimen tilting
process and will need beam blanking to avoid specimen damage. Can
anyone confirm or deny all this?

Thanks in advance for your help,

Chris



From daemon Thu Mar 09 14:30:51 2000



From: Catherine Ripley :      ripley-at-aecom.yu.edu
Date: Thu, 09 Mar 2000 10:26:55 -0500
Subject: Confocal listserver

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Thanks all.
It was just some anomaly. It went through the second time.


Catherine Ripley
*****************************************
Albert Einstein College of Medicine
1300 Morris Park Avenue
Bronx, NY 10461
*****************************************
*****************************************



From daemon Thu Mar 09 14:30:52 2000



From: Tim E. Harper :      tim-at-cmp-cientifica.com
Date: Thu, 9 Mar 2000 16:51:12 +0100
Subject: Conference Announcement: EuroFE 2000

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Dear all,

EuroFE 2000 is a forum to bring together interested parties in the field of
Field Emission Technologies. This includes microscopists working with
materials used for field emission sources (DLC, Nanotubes etc.) and those
developing field emission based sources for SEM & TEM.

There is also a strong emphasis on links with other display technologies
such as LCD's, Light Emitting Polymers and Phosphors.

The conference and workshop sessions will build on the success of EuroFE '99
& will be held in the UNESCO World Heritage city of Segovia.

The aim of this conference will be to focus on the European dimension of
Field Emission, on Industrial Problems and to bring together in a Forum the
various groups (from Industry and Public Institutions) working in this area.
In addition to the normal program of keynote speakers, oral presentations
and poster sessions, it is also planned to hold workshops on topics such as
"overcoming obstacles to industrial production".

In common with EUROFE'99 conference, there will be an emphasis on the
applications of field emission technologies, as well as basic scientific
research. A crucial issue during a conference is time allowed to discussions
between participants in order to exchange ideas and therefore possibly
define strategies in order to solve real problems. During EUROFE'2000,
following each session, large breaks will be set-up in order to allow these
discussions.

One of the major sessions will be related to Field Emission applications
such as future big FE flat panel displays (1m2). Companies such as Motorola,
Samsung, PixTech, Candescent and Saint-Gobain will participate actively in
this session. The "BigFED" project coordinated by EUROFE will be presented
at the conference in order to define strategies to be able to present
projects to the EU within this objective.

The key topics of interest will be:


Industrial applications (Flat panel displays, etc.) and related
problems (reliability, lifetime, etc.)
Field Emission from diamond, DLC and nanotubes
FE simulation and modelling
Understanding Field Emission
Space applications
Device characterisation (surface analysis, etc.)
Novel cathodes - technology and fundamentals
Other related areas: (Phosphors, LCDs, OLEDs, CRTs, New materials,
Novel devices, etc.).

An important issue during conferences is to make possible student
participation in order to form them and allow initiation of contacts with
either industry or public institutions. For this reason, this year, the
EUROFE2000 organisation set-up a special reduced registration fee for
students including also the accommodation.


Regards

Tim


******************************************************************
Tim E. Harper EuroFE Network Co-Chairman
EuroFE is a network of the European Science Foundation
Phone +34 91 640 71 85 Fax +34 91 640 71 86





From daemon Thu Mar 09 14:30:52 2000



From: Gib Ahlstrand :      giba-at-puccini.cdl.umn.edu
Date: Thu, 9 Mar 2000 09:56:57 -0600 (CST)
Subject: Re: Rapid Fixation of cells for SEM)

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Responding to the message of {v03007805b4ec9d6d6eae-at-[206.69.208.21]}
from R schlicher {rkslick-at-yahoo.com} :

} I need to fix cells for viewing with SEM. However,
} the fixation method needs to be consistent with my
} experimental methods:
}
} 1) I need to fix the cells in less than 1 minute and
} due to this time limitation, I cannot spin the cells
} into a pellet and must add the cell suspension to the
} fixative; and

snip!

I would like to try chemical
} fixation and SEM. It has been suggested that I try
} glutaraldehyde + buffers, then postfix with osmium
} tetroxide and dehydrate in an ethanol series.

Robyn,

You could try simultaneous glutaraldehyde + osmium fixation, which should work
well for cells in suspension in particular. Mix up seperate solutions of each,
such that when mixed in equal amounts, you get the concentrations of fixes and
buffers you want. Its usually recommended that you do this at lower
temperatures, like about 4C, so pre-cool the solutions and vials first, to
prevent the glut and osmium from fighting with each other, but even for "less
than a minute", as you put it, maybe you could still work at room temperature.
Try mixing the two fixatives together without cells first, to determine the
maximum time until discoloration occurs, which means the osmium is precipitating
out or whatever goes on there.

Your first rinse could just be a large dilution, to seperate the warring
fixatives, then spin down and rinse a few more times before going into the
dehydration series.

Give me a few hours to dig out references for this technique, will send them out
later.

Gib






Gib Ahlstrand
Electron Optical Facility, University of Minnesota, Dept. Plant Pathology
495 Borlaug Hall, St. Paul, MN. USA. 55108 (612)625-8249
612-625-9728 FAX, giba-at-puccini.cdl.umn.edu
http://biosci.umn.edu/MIC/consortium.html



From daemon Thu Mar 09 14:30:53 2000



From: Gib Ahlstrand :      giba-at-puccini.cdl.umn.edu
Date: Thu, 9 Mar 2000 10:44:28 -0600 (CST)
Subject: Re: Rapid Fixation of cells for SEM)

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Robyn,

Here are two references for the simultaneous glut/osmium technique I mentioned
earlier.


1. Franke, W.W., Krien, S. & Brown, J.R.M. (1969) Simultaneous glutaraldehyde
osmium tetroxide fixation with post osmication. Histochemie, 19, 162-164.

2. Hirsch, J.G. & Fedorko, M.E. (1968) Ultrastructure of human leukocytes after
simultaneous fixation with glutaraldehyde and osmium tetroxide and post fixation
in uranyl acetate. J. Cell Biol. 38, 615-627.


Good luck!

Gib Ahlstrand


Responding to the message of {v03007805b4ec9d6d6eae-at-[206.69.208.21]}
from R schlicher {rkslick-at-yahoo.com} :

} Hello,
}
} I need to fix cells for viewing with SEM. However,
} the fixation method needs to be consistent with my
} experimental methods:
}
} 1) I need to fix the cells in less than 1 minute and
} due to this time limitation, I cannot spin the cells
} into a pellet and must add the cell suspension to the
} fixative; and
}
} 2)I am looking for "effects" to the cell membrane
} caused by my experiment, so I need to keep any
} possible fixative damage/effects at a minimum.
}
} I have fixed cells using plunge freezing and viewed
} them with TEM, this is time consuming and I ran into
} a
} lot of freeze damage. I would like to try chemical
} fixation and SEM. It has been suggested that I try
} glutaraldehyde + buffers, then postfix with osmium
} tetroxide and dehydrate in an ethanol series.
}
} Is this the best method? I am open to suggestions.
} I am using suspensions of prostate cancer cells in
} RPMI (+serum) growth media; I can perform the
} experiment in buffered saline and use a high
} concentration of cells.
}
} Thanks,
} Robyn
}
}
} Robyn K. Schlicher, M.S.
} Laboratory for Drug Delivery
} Institute for Bioengineering and Biosciences
} Georgia Institute of Technology
} 315 Ferst Drive
} Atlanta, GA 30332
} rkslick-at-yahoo.com


Gib Ahlstrand
Electron Optical Facility, University of Minnesota, Dept. Plant Pathology
495 Borlaug Hall, St. Paul, MN. USA. 55108 (612)625-8249
612-625-9728 FAX, giba-at-puccini.cdl.umn.edu
http://biosci.umn.edu/MIC/consortium.html



From daemon Thu Mar 09 14:30:55 2000



From: Leona Cohen-Gould :      lcgould-at-mail.med.cornell.edu
Date: Thu, 9 Mar 2000 13:44:11 -0400
Subject: Re: methods of embedding cells cultured on glass coverslips

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At 6:09 PM -0600 3/8/0, Jaci Lett wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Your are left with a mirror-finish block face that is perfectly intact.
Even with all the safety precautions, I find this method preferable and
more reliable in my hands than the "heat and snap-off" method that many
people use.

Just don't let your students do it!

Good luck,
Lee



Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Confocal Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175




From daemon Thu Mar 09 14:30:57 2000



From: Kristen Lennon :      kalen-at-citrus.ucr.edu
Date: Thu, 09 Mar 2000 10:04:34 -0800
Subject: immunofluorescence in methacrylate

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Dear microscopists,
Can anyone offer advice on doing immunofluorescence at the light level? I
have cryopreserved plant tissue that I am contemplating embedding and using
for immunolocalization. I have a protocol that uses butyl, methyl
methacrylate. I am wondering several things. Why butyl, methyl
methacrylate? Are there other resins/media that are sufficient? I'd
appreciate any advice anyone has.
Kristen Lennon
Kristen A. Lennon
Cell, Molecular & Developmental Biology Group
Department of Botany & Plant Sciences
University of California
Riverside, CA 92521
kalen-at-citrus.ucr.edu



From daemon Thu Mar 09 14:30:57 2000



From: Eric Windsor :      Eric.Windsor-at-nist.gov
Date: Thu, 09 Mar 2000 10:20:15 -0500
Subject: Mat: Elemental Boron Manufacturers

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I have a question that is not a typical microscopy question but I thought I
would call upon the extensive knowledge of the readers of this list server.

Does anyone know who manufacturers elemental boron?

We are using boron as a substrate for particle microanalysis (especially
carbonaceous particles). Elemental boron can be purchased in the form of
“lumps” or “nuggets” several centimeters in dimension from chemical supply
houses such as Alfa Aesar and Aldrich. These companies however will not
reveal their suppliers and so any questions that I want to ask the
manufacturer have to go through the third party supply house. In addition
to being silly, this procedure is extremely slow and inefficient. I would
prefer to bypass the third party altogether.

So if anyone has any idea who manufacturers elemental boron I would greatly
appreciate hearing from you.

Disclaimer: Any opinions expressed are my own and not those of my
employer, The Federal Government.


Eric Windsor

Eric S. Windsor
Physical Scientist
NIST
100 Bureau Dr. Stop 8371
Gaithersburg, MD 20899-8371
(301) 975-3930
Fax: (301) 417-1321
Eric.Windsor-at-NIST.Gov



From daemon Thu Mar 09 14:30:58 2000



From: Tamara Howard :      howard-at-cshl.org
Date: Thu, 9 Mar 2000 13:08:37 -0500 (EST)
Subject: TEM, bio, Ag/Au enhancement

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Greetings!

One of the researchers here is trying to do silver and/or gold enhancement
of an immunolabel in brain slices, but is getting amazing background. We
switched to the gold enhancement because everyone was saying how much
cleaner it is....didn't help. I've seen one report in which the
researchers pre-washed their samples in EDTA, because they felt that they
were getting background from Mg in the tissue that was nucleating silver
deposition - anyone else have any thoughts/experience on this? Anyone else
doing enhancement in tissue slices?

He is using kits to do the enhancement - I'm not sure which companies are
the suppliers, but they aren't supposed to be light-sensitive (my first
culprit). The samples are washed extensively in water before enhancement,
and even the no-antibody controls are "lighting up".

Any suggestions will be greatly appreciated!

Tamara Howard
CSHL



From daemon Thu Mar 09 14:31:00 2000



From: Alwyn Eades :      jae5-at-lehigh.edu
Date: Thu, 09 Mar 2000 13:49:03 -0500
Subject: Re: TEM: Electron Tomography

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Chris,

I do not have the information you need but I am old enough to remember
seeing one of the first papers on tomography. It chose a cute title,
something like:
"Algorithm for Reconstructive Tomography or ART"
Which was fine until the next issue of the journal had a paper (giving an
improvement on the method) called:
"Fast Algorithm for Reconstructive Tomography."

Good luck,
Alwyn Eades



From daemon Thu Mar 09 14:31:03 2000



From: Michael Bode :      mb-at-soft-imaging.com
Date: Thu, 9 Mar 2000 11:58:54 -0700
Subject: RE: Transmission electron microscopy

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Peter,

a transmission electron microscope is in principle the same as a
transmission light microscope, except that it uses high energy electrons
instead of light. This allows a much higher resolution (it is possible
to see atoms or cyrstalline structures), but necessitates much bigger
instruments, as there is vacuum technology, high voltages and X-ray
generation involved. Many different techniques are available on these
machines.

Rather than explaining all to you, you could do a search on the web
looking for "transmission electron microscopy" or "TEM". Another
starting point would be to go to

http://ncmi.bcm.tmc.edu/

and select one of the sites under "Links". Or go to the MSA site

http://www.msa.microscopy.com/

and find your way from there. I am sure people will help you here if you
have specific questions.

Michael

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



} ----------
} From: JUPE Peter[SMTP:JUPE.PETER-at-HDH.COM.AU]
} Sent: Monday, March 06, 2000 8:35:46 PM
} To: 'Microscopy-at-MSA.Microscopy.Com'
} Subject: Transmission electron microsocopy
} Auto forwarded by a Rule
}
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The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Dear Sir
Could you please supply me details on "transmission electron
microsocopy"
for my 16yr old daughter at high school, or alternatively where to look
on
the Internet.
Thanking you
Peter Jupe



From daemon Thu Mar 09 14:31:03 2000



From: JoAnn Buchanan :      redhair-at-leland.Stanford.EDU
Date: Thu, 09 Mar 2000 11:27:43 -0800
Subject: Re: methods of embedding cells cultured on glass coverslips

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Dear Jaci. If you wish to have access to all the cells on the coverslip,
the best way is to flat embed the whole coverslip in a Chang monolayer mold
(20mm or 10mm) available from EMS. Be sure to drain the coverslips well
and wipe excess resin from the bottom of the coverslip. Invert over filled
mold and polymerize overnight. Remove from oven and use metal file to file
down edges of embedded coverslip, then immerse in concentrated hydrofluoric
acid (use plastic forceps, work in fume hood, wear nitrile glove sand rinse
everything well with water). It will dissolve the glass in 15-30 mins. It
works every time and you have a complete embedded coverslip. You can then
stain it with toludine blue, find the identified cell, mark it with a Ladd
marking objective. I then punch out the cell(s) of interest using a hole
punch and attach the 5mm circles to blank stubs using quick setting
epoxi-patch bond (hardens in 5-10 minutes). This is available from Dexter
Corporation 1-603-474-5545 and works extremely well. I sometimes have low
contrast so Alexander Mironov's washing method may solve this problem.
Otherwise, stain for 30 mins in 5% aqueous UA and 3 mins in Lead citrate
for good results. Good luck, JoAnn Buchanan



From daemon Thu Mar 09 14:31:05 2000



From: hadden-at-wingate.edu (Lee Hadden)
Date: Thu, 09 Mar 2000 14:51:27 -0500
Subject: suggestions for new SEM??

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When I stepped outside of the science building tonight, I ran into,
literally, our university president who said he was just walking over to
try to find me. He cautioned me about overreacting and then proceeded to
tell me to go ahead and get my best quote on a new SEM and service
contract!!!!!!

Now that I've got both feet back on the ground, I need any help that any
of you can provide, based on your experience and knowledge. We're
looking for a new SEM that will support undergraduate courses, be a good
training machine for students, support student and faculty research and
have a good track record for reliability and being reasonably user
friendly. It should be state of the art, but not be so expensive to
operate and keep up that it would limit how much students and faculty
could use it.

I was trained on an Amray in the 80's and ran our TEM until it became
cost ineffective a few years ago. But I know things have changed
considerably since my pre-computer controlled everything SEM's and would
appreciated the benefit of any experience any of you might have had with
new SEMS.

Anything to recommend? Models? Features to insist on or avoid?
Reliability, service costs and frequency? Companies to go with or stay
away from? What new models do you have and would you recommend them to
others? Why or why not?

I'd really appreciate any and all insight you can provide. When your
president walks to YOUR office and says the equipment grant proposal HE
made to a major foundation was just approved and that he needs me to get
the best quote on an SEM to him ASAP, I want to have something to go on,
and reasons to go along with it based on user input, without delay.

Thanks in advance for any assistance you can provide.

Lee Hadden
Professor and Chair,
Department of Biology
Wingate University
Wingate, NC 28174

hadden-at-wingate.edu
http://www.wingate.edu
704-233-8238




From daemon Thu Mar 09 18:37:32 2000



From: Claudia Hayward-Costa :      LS_S562-at-crystal.kingston.ac.uk
Date: Thu, 9 Mar 2000 20:28:12 +0100
Subject: UV spectra of Glutaraldehyde

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I am only using distilled Glutaraldehyde - and only up to 7 days
after making up the fixative (Karnovsky's) and storing it in the fridge.

UV Spectra of pure Glutaraldehyde show a single absorbance peak
at 280 nm (monomeric ) - whereas the commercial material shows
an additional stronger peak at 235nm. This is assumed to be due
to the formation of oligomer and polymer.
The ratio of absorbance at 235 and 280 nm can be used as a
measure of the quality of the GA.

Monomeric-polymeric mixtures yield good ultrastructural
preservation when the ratio of monomeric to polymeric forms is 1:1
or 1:2. Ratios outside this limits may prove unsatisfactory.
(Weakley 1974)
(unfortunately I do not have the exact source/ Paper handy where I
got that from.)


Dr. C. Hayward-Costa
School of Life Sciences
Kingston University
Penrhyn Road, Kingston upon Thames
Surrey KT1 2EE, UK
44(0)181 547 2000 x 2240
Email: c.hayward-at-kingston.ac.uk



From daemon Thu Mar 09 18:37:33 2000



From: hank adams :      hpadams-at-bcm.tmc.edu
Date: Thu, 9 Mar 2000 14:41:30 -0600
Subject: glass coverslip detachment

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I have 95% success detaching glass coverslips embedded with epon inverted on beem capsules described by Leona by simply placing the polymerized coverslip on a hotplate ( } 85.C) with the beem capsule up and gently prying them apart with a straigtedge razor. Cells stay where you want them. I found by that instead of using the beem capsules I just use the lid of one or the lid of a microfuge tube ( makes a better platform). Once separated put the top on a slide and the cells are easily visualized with brightfield (osmicated) or phase (non osmicated).
Hank Adams
Integrated Microscopy Core
Molecular and Cellular Biology
Baylor College of Medicine
Houston, TX



From daemon Thu Mar 09 18:37:36 2000



From: Paul Webster :      pwebster-at-mailhouse.hei.org
Date: 09 Mar 00 12:57:58 -0800
Subject: Re: methods of embedding cells cultured on glass coverslips

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Reply to: Re: methods of embedding cells cultured on glass coverslips
Growing cells on coverslips, fixing them in situ and processing them into epoxy resin is fairly straight forward. Remember that the cells are a monolayer and are only about 30 microns thick so fixation, rinsing, dehydration and infiltration times can be cut down considerably.

The final step of removing the coverslip from the polymerized block is not, however, as trivial as everyone makes it out to be. Yes, dipping the warm block in liquid nitrogen and then re-warming it, will eventually take the glass off the plastic (leaving the cells in the block), but only if the thin layer of plastic that always seems to appear on the back of the glass is removed.
The block and glass are also more easily removed if the resin is polymerized with the glass resting cell-side down on resin. The alternative way, of immersing the coverslip cell-side up, and letting the resin cover the cells, is safer, but leads to problems in removing the glass.

If there is a problem with removing the glass, making scratches with a diamond scribe can help and sometimes bending the whole block and glass coverslip can be useful ( if this is possible).

Don't worry about immersing the block in the nitrogen either, there really doesn't seem to be just one way that this happens. If the process is able to remove the glass, the glass may shoot off so violently you may wish you had put on your safety glasses. The next block may not work.

One other method I heard of was to put the block, glass-side down, on a hot plate. Wait until the glass gets hot and I'm told it will easily slide off. I never tried it.
Paul Webster, Ph.D
House Ear Institute
2100 West Third Street
Los Angeles, CA 90057
phone:213 273 8026
fax: 213 413 6739
e-mail: pwebster-at-hei.org
http://www.hei.org/htm/aemi.htm


Alexander Mironov wrote:
}
} Dear Jaci,
} The problem of cell embedding after their cutivation on plastic or galss
} is rather simple. You do not need to use Thermanox. You can use glass. The
} trick is that immediately after polymerization you have place glasses at } -20 degree C and your samples will be detached. If thsi scheme does not
} work you can use liquid nitrogen. However, do not insert smplaes into it
} (only glass). If this scheme does not work you can use commercial solution
} of HF (acid). It dissolve glass efficiently. For instance, coverslips are
} dissolved in 30 min. Then you have to wash samples in buffer (pH7.4) for a
} long time and water (several hours) otherwise you will have problems with
} contrasters. } We usually glue samples after detachment from glass and work with
} this sandwich. For this you have to use incomplete polymerization of Epon
} on chamber slides (12 hours). Then samples can be detached at -20 and then
} glued with the fresh resin for 24 hours. If you use full polymerization
} time on glass or any polymerization on plastic you will not be able to
} glue samples.
}
} Sincerely yours, Alexander Mironov
} Italy
}
}
} On Wed, 8 Mar 2000, Jaci Lett wrote:
}
} } I know this has been covered periodically, but as I've not needed the
} } technique, I've not paid attention.  I need some methods of embedding
} } cells cultured on glass coverslips.  Obviously, the most important
} } issue is removing the coverslip from the polymerized block.I've looked
} } through my own reference materials and the only method I found involved
} } using Thermanox coverslips (upon which these particular cells will not
} } grow, I'm informed).Please reply dircectly to my email address as well as
} } to the Listserver (I haven't received postings for several days). Thank
} } you,Jaclynn M. Lett, Research Assistant    
} } jmlett-at-cid.wustl.eduFay and Carl Simons Center for Biology of Hearing and
} } DeafnessCentral Institute for the Deaf4560 Clayton AvenueSt. Louis,
} } MO  63110voice:  314-977-0257     fax: 
} } 314-977-0030
} }

Paul Webster, Ph.D
House Ear Institute
2100 West Third Street
Los Angeles, CA 90057
phone:213 273 8026
fax: 213 413 6739
e-mail: pwebster-at-hei.org
http://www.hei.org/htm/aemi.htm



From daemon Thu Mar 09 18:37:39 2000



From: William Tivol :      tivol-at-wadsworth.org
Date: Thu, 09 Mar 2000 16:02:21 -0500
Subject: Re: TEM: Electron Tomography

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Chris Adams wrote:

Dear Chris,


} I have recently been asked to participate in an effort at Los Alamos
} concerned with Electron Tomography of polymers. I haven't done the
} required image reconstruction before. Does anyone know of any
} commercially available software that will reconstruct several 2D
} images taken at various tilt angles into a single 3D reconstruction
} of an object?

Yes. SPIDER is commercially available, and will do 3D
reconstructions by any of several methods. Check it out at
www.wadsworth.org; click on Resources (pretty far down on the
left-hand side); then click on SPIDER.


} I suppose it would be analogous to the software used
} for CAT scans?

Some of the reconstruction methods, at least, are used in
CAT scans, but others (e.g., projection onto convex sets) may not
be--I don't know the latest software for CAT scans.


} Also, if you've done this before, what kind of
} hardware requirements are there?

We run on an Onyx using unix; I don't know all the available
versions of SPIDER.


} I've got a JEOL 2010 configured at
} the moment to allow only ±30 degrees of tilt. Is that enough?

No. +/- 50 deg is adequate, +/- 60 deg is better.


} I assume I also need some way of automating the specimen tilting
} process and will need beam blanking to avoid specimen damage. Can
} anyone confirm or deny all this?
}

Automation is optional, but beam blanking is mandatory--
especially for cryo-specimens. If your goniometer is sufficiently
accurate, you can change the tilt setting with the beam blanked,
then adjust the position and focus quickly after the beam is back
on. If you don't have a sufficiently good goniometer, you need
to have either a low-dose kit (assuming you can recognize both
your area of interest and a nearby area for focusing) or a very
sensitive video-rate camera, such as the intensified CCD we have
on the HVEM, so you can find and focus the area of interest with
minimal exposure of the specimen.


}
} Thanks in advance for your help,
}

You're welcome.
Yours,
Bill Tivol



From daemon Thu Mar 09 18:37:43 2000



From: Tobias Baskin :      BaskinT-at-missouri.edu
Date: Thu, 9 Mar 2000 16:41:52 -0600
Subject: Re: immunofluorescence in methacrylate

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Kristen,
I have published several papers on using
butylmethylmethacrylate for immuno at the light level, including a
paper where cryofixation and freeze substituion were used, on plant
material. Perhaps the protocol you have is by me?

To answer your questions, a mixture of butyl and methyl
methacrylate gives nice blocks and preserves the tissue well. I have
tried using pure butyl or pure methyl and the sectioning or
preservation was worse. These resins, alone or in combination, have
the crucial advtange of being mostly removable post embedment with a
brief incubation in actetone. This improves the access of your
antibody to your antigen greatly. It is important when doing immuno
work at the light level on sections to remember that if an antibody
can penetrate say 15 nm into the plastic (I am guessing at this
number), this is most of the thickness of an ultra thin section but
only a teeeny bit of a semithin section. So, for light work
especially, being able to remove the embedment is crucial. The usual
way to do this is to embed in paraffin and this is fine if you just
want tissue level distinctions. But if you want subcellular
localization or for other reasons you want your stuff to be better
preserved, then plastic gives much nicer results.
DOes this help?
I will be quite happy to help you further with butylmethyl
methacrylate, as I have a professional fondness for the stuff.
Good luck,
Tobias

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
_ ____ ^ __ ____ Tobias I. Baskin
/ \ / / \ / \ \ University of Missouri
/ | / / \ \ \ Biological Sciences
/___/ /__ /___ \ \ \__ 109 Tucker Hall
/ / / \ \ \ Columbia, MO 65211-7400 USA
/ / / \ \ \ voice: 573-882-0173
/ /____ / \ \__/ \____ fax: 573-882-0123



From daemon Thu Mar 09 18:37:45 2000



From: DDittus787-at-aol.com
Date: Thu, 9 Mar 2000 17:56:03 EST
Subject: Re: TEM, bio, Ag/Au enhancement

Contents Retrieved from Microscopy Listserver Archives
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Dear Tamara
Is he washing in de-ionized water? Brain is always absorbent, so timing and
reagent volumes may need adjustment. Gold and silver enhancement are tricky
so I would protect from heat and light as much as possible. Good Luck.
Dana



From daemon Thu Mar 09 18:37:47 2000



From: AMaterEval-at-aol.com
Date: Thu, 9 Mar 2000 18:24:42 EST
Subject: TOPSCAN Confocal Microscope Availability

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We are looking for someone to provide analytical services on analyzing
surface topography on biomedical implants specifically using a TOPSCAN
confocal microscope. It needs to be performed on this instrument for
comparison with data previously taken on such an instrument. Does anyone
know of anyone who has this instrument and who might entertain performing
such an analysis? Your help on this will be greatly appreciated.

Charles R. Anderson, Ph.D.
President
Anderson Materials Evaluation, Inc.
1450 South Rolling Road
Halethorpe, MD 21227
(410) 455-5698
Fax (410) 455-5679

An independent materials analysis laboratory for failure analysis, quality
control, product and process development. We offer small-spot XPS, Auger
microprobe, AFM, SEM, optical metallographic microscopy, white light
interference microscopy, mass spectroscopy, and thermal analysis (TGA, DSC,
TMA, DMA) services.



From daemon Thu Mar 09 18:37:50 2000



From: PESTOEM-at-aol.com
Date: Thu, 9 Mar 2000 18:50:23 EST
Subject: Objective Aperture Moving

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


To All:
We have a Jeol 100CX II and experiencing a constant moving of the Objective
Aperture moving when scanning grids, especially in Posistion # 2 Grid.
Any solution or ideas? We changed apertures,cleaned holder etc.
Thank you,
Peter Stolzenberg, PESTO INC.
pestoem-at-aol.com



From daemon Thu Mar 09 18:37:51 2000



From: Walck. Scott D. :      walck-at-ppg.com
Date: Thu, 9 Mar 2000 19:00:13 -0500
Subject: RE: suggestions for new SEM??

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We just bought a new top-end type microscope (Field emission semi-inlens
SEM). I evaluated four companies. I would have been happy with any of the
microscopes that I looked at. In my opinion, all of the microscopes
available today are damn good throughout the product range. You will
probably be happy with any microscope that you buy. If your budget is
limited and defined as ours was, that is going to limit you as to what
machine that you will buy. You can use that to see what the manufacturers
will put into your package. There are other intangibles that go into the
decision. The most important is service, in particular, the service in your
area. Ask around! I did and got some interesting comments. Then there are
some of the others that you have to judge for yourself. Things like the
user interface -you will be training students to use it, you want a
versatile machine, but one that is easy to learn to use; output
capabilities; digital/film or both; networkability; etc.

If performance is the issue, then the best thing that you can do is to take
a number of your samples and take them to the application labs and run them
while you are there. You should take samples that are representative of the
types of samples that you will be working with. Make sure that you compare
the same conditions on all your samples and that the output that you get
back can be compared at the same magnifications. Standardize the output
that you want the images to be in, for example 8 bit grayscale TIFF images
or whatever. You should also do the samples in the same order from lab to
lab. Print the results from digital images on the same printer. Then get a
group of experienced users to help you judge the results.

-Scott


Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)


"The opinions expressed are those of Scott D. Walck and not of PPG
Industries, Inc. nor of any PPG-associated companies."

--

} -----Original Message-----
} From: hadden-at-wingate.edu [ mailto:hadden-at-wingate.edu
{mailto:hadden-at-wingate.edu} ]
} Sent: Thursday, March 09, 2000 2:51 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: suggestions for new SEM??
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
{http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html}
} --------------------------------------------------------------
} ---------.
}
}
} When I stepped outside of the science building tonight, I ran into,
} literally, our university president who said he was just
} walking over to
} try to find me. He cautioned me about overreacting and then
} proceeded to
} tell me to go ahead and get my best quote on a new SEM and service
} contract!!!!!!
}
} Now that I've got both feet back on the ground, I need any
} help that any
} of you can provide, based on your experience and knowledge. We're
} looking for a new SEM that will support undergraduate
} courses, be a good
} training machine for students, support student and faculty
} research and
} have a good track record for reliability and being reasonably user
} friendly. It should be state of the art, but not be so expensive to
} operate and keep up that it would limit how much students and faculty
} could use it.
}
} I was trained on an Amray in the 80's and ran our TEM until it became
} cost ineffective a few years ago. But I know things have changed
} considerably since my pre-computer controlled everything
} SEM's and would
} appreciated the benefit of any experience any of you might
} have had with
} new SEMS.
}
} Anything to recommend? Models? Features to insist on or avoid?
} Reliability, service costs and frequency? Companies to go
} with or stay
} away from? What new models do you have and would you
} recommend them to
} others? Why or why not?
}
} I'd really appreciate any and all insight you can provide. When your
} president walks to YOUR office and says the equipment grant
} proposal HE
} made to a major foundation was just approved and that he
} needs me to get
} the best quote on an SEM to him ASAP, I want to have
} something to go on,
} and reasons to go along with it based on user input, without delay.
}
} Thanks in advance for any assistance you can provide.
}
} Lee Hadden
} Professor and Chair,
} Department of Biology
} Wingate University
} Wingate, NC 28174
}
} hadden-at-wingate.edu
} http://www.wingate.edu {http://www.wingate.edu}
} 704-233-8238
}
}
}



From daemon Thu Mar 09 18:37:57 2000



From: Hong Yi :      hyi-at-emory.edu
Date: Thu, 9 Mar 2000 19:29:00 -0500 (EST)
Subject: Re: TEM, bio, Ag/Au enhancement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Tamara:
To figure out what went wrong with your investigator's
experiment, we need more detail about how his experiment was conducted.
Would you ask him to contact me and email his protocol to me? I believe
that I can help him.

Hong
==============
Hong Yi
Emory Neurology
hyi-at-emory.edu

On Thu, 9 Mar 2000, Tamara Howard wrote:

} ------------------------------------------------------------------------
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} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Greetings!
}
} One of the researchers here is trying to do silver and/or gold enhancement
} of an immunolabel in brain slices, but is getting amazing background. We
} switched to the gold enhancement because everyone was saying how much
} cleaner it is....didn't help. I've seen one report in which the
} researchers pre-washed their samples in EDTA, because they felt that they
} were getting background from Mg in the tissue that was nucleating silver
} deposition - anyone else have any thoughts/experience on this? Anyone else
} doing enhancement in tissue slices?
}
} He is using kits to do the enhancement - I'm not sure which companies are
} the suppliers, but they aren't supposed to be light-sensitive (my first
} culprit). The samples are washed extensively in water before enhancement,
} and even the no-antibody controls are "lighting up".
}
} Any suggestions will be greatly appreciated!
}
} Tamara Howard
} CSHL
}
}





From daemon Fri Mar 10 07:17:23 2000



From: Milo Kral :      m.kral-at-mech.canterbury.ac.nz
Date: Fri, 10 Mar 2000 15:30:46 +1300
Subject: Hitachi H-600 Parts Needed

Contents Retrieved from Microscopy Listserver Archives
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Does anyone have a scrapped CRT (with circuit board) for data display that
they can part with? Ours has given up finally.....

Also, I was wondering if there were any hot or cold stages still out there
for the old H-600.

We are happy to pay for these things if they turn up somewhere.

Thanks
Milo Kral
University of Canterbury
Department of Mechanical Engineering
Christchurch
New Zealand




From daemon Fri Mar 10 07:17:31 2000



From: Rick Powell at Nanoprobes :      rpowell-at-nanoprobes.com
Date: Thu, 9 Mar 2000 23:22:58 -0500
Subject: Re: TEM, bio, Ag/Au enhancement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Tamara:

The topic of reducing background has been raised a number of times by our
customers, and we have collected together several approaches and their
references, and I hope some of the following are helpful:

One possible mechanism for "background" signal is hydrophobic interactions
between gold particles and tissue components, and if your samples can
tolerate them, additives which break these interactions down and solubilize
hydrophobic species might help in reducing background. Washes which
effectively solubilize gold compounds and other hydrophobic species
include:

* 0.6 M triethylammonium bicarbonate buffer (prepared by bubbling
CO2 into an aqueous suspension of triethylamine with stirring;
for a reference, see Safer, D.; Bolinger, L., and Leigh, J. S.;
J. Inorg. Biochem., 26, 77 (1986)).

* A small amount of detergent, such as Tween-20 or Triton X-100,
or an amphiphile such as benzamidine or 1,2,3-trihydroxyheptane.

* If you are using the gold enhancer, raising the ionic strength
of the solution is sometimes helpful - we have found that
raising the sodium chloride concentration in the actual gold
enhancement mixture to 0.5 M can actually reduce the background.
Alternatively, lowering the pH of the gold enhancement mixture
by about 0.5 pH units may help.

Acetonitrile coordinates quite well to silver ions, so if your background
problem is due to the interaction of silver from the enhancement reagents
with a component of your tissues, treatment with acetonitrile or a wash
solution containing a proportion of acetonitrile may help (provided the
sample can withstand it).

To reduce the background for silver enhancement, one procedure which has
been found to be effective is to wash with 0.02 M sodium citrate buffer, pH
7.0, several times immediately before silver enhancement - this has been
used to reduce background in experiments with the combined fluorescein and
gold probe FluoroNanogold when used with HQ Silver (Nanoprobes). When the
Danscher formulation of silver enhancer was used instead, 0.02 M sodium
citrate buffer at pH 3.5 was found to be most effective. In immunoblots, we
have also observed that washing with 0.05 M disodium EDTA, pH 4.6, before
silver enhancement also results in low background. (Reference: Powell, R.
D.; Halsey, Carol M. R.; Spector, D. L.; Kaurin, S. L.; McCann, J.;, and
Hainfeld, J. F. A covalent fluorescent-gold immunoprobe: "simultaneous"
detection of a pre-mRNA splicing factor by light and electron microscopy.
J. Histochem. Cytochem., 45, 947-956 (1997)).

A number of methods have been described for stopping the silver enhancement
reaction or for "back-developing" to remove extraneous deposited silver.
These prevent the continuation of the reaction in the specimens after
development is complete (for example, if the silver is only slowly removed
from the tissue), and may help reduce your background signal.

Sodium thiosulfate (1 % aqueous solution, freshly made) is a good "stop"
reagent for both silver and gold deposition (Van Driel, D. 1997. Gold
toning for silver enhanced immunogold reacted tissue. Micros. Today, 97-7,
28), so it is reasonably safe to assume that there will be no further
deposition after it is applied. However, one caveat: you should use caution
with gold enhancement because there have been reports that sodium
thiosulfate can remove the enlarged gold particles. This might be a good
choice to begin with (1-2 minute incubation after enhancement and washing
with water; after the sodium thiosulfate, rinse thoroughly again with
deionized water).

Other reaction stop and back development methods include:

(i) 1% acetic acid (Scopsi, L. 1989. Silver-enhanced colloidal gold
method., p. 260. In M. A. Hayat (ed.), In Colloidal Gold: Principles,
Methods, and Applications, vol. 1. Academic Press, San Diego, CA).

(ii) 1% acetic acid followed by photographic fixer (Agefix, Agfa-Gevaert,
or Ilfospeed 200, Ilford) (Scopsi, L. 1989., same reference as (i)).

(iii) direct photo fix, using those just mentioned (Burry, R.W. 1995.
Pre-embedding immunocytochemistry with silver-enhanced small gold
particles, p. 217-230. In MA Hayat (Ed.). Immunogold silver staining:
Principles, methods and applications. CRC Press, Boca Raton).

(iv) brief rinse in 2.5% sodium chloride (Scopsi, L. 1989., same reference
as (i)).

(v) 15-25% aqueous sodium thiosulfate plus 15% sodium sulfite (Danscher, G.
1981. Histochemical demonstration of heavy metals. A revised version of the
silver sulphide method suitable for both light and electron microscopy.
Histochemistry 71, 1-16).

(vi) 1% acetic acid, washes in acetate buffer, toning in 0.05% HAuCl4 3-10
min, with excess silver removed with 3% sodium thiosulfate. We found that
Nanogold-labeled proteins run on a polyacrylamide gel kept low backgrounds
when stopped with 10% acetic acid with 10% glucose in water, as opposed to
just a water stop (Takizawa, T. and J.M. Robinson. 1994. Use of 1.4-nm
immunogold particles for immunocytochemistry on ultra-thin cryosections.
J. Histochem. Cytochem. 42, 1615-1623).

(vii) A modified Farmer's solution was used for the reversal (0.3 ml 7.5%
potassium ferricyanide, 1.2 ml of 20% sodium thiosulfate, 60 ml water)
(Danscher, G.: Histochemistry, 71, 1-16 (1981)). Application of this
solution briefly to your sample before gold toning may help to remove
background silver deposition.

Hope this helps,

Rick Powell


**********************************************************************
* - PLEASE NOTE OUR NEW ADDRESS - EFFECTIVE FEBRUARY 15, 2000 - *
* *
* NANOPROBES, Incorporated | US Toll-free: (877) 447-6266 *
* 95 Horse Block Road | Tel: (919) 510-0590 *
* Yaphank, NY 11980-9710, | Fax: (919) 510-0590 *
* USA | rpowell-at-nanoprobes.com *
* *
* NOW EASY TO FIND ON THE WEB: http://www.nanoprobes.com *
**********************************************************************




From daemon Fri Mar 10 07:17:42 2000



From: Vitaly Feingold :      vitalylazar-at-worldnet.att.net
Date: Fri, 10 Mar 2000 02:10:24 -0500
Subject: Looking for EDS system.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers'

Please help this gentleman. He needs inexpensive EDS system suitable for
Philips TEM (horizontal entrance detector) complete, and old EDAX-9800 or
similar, computer unit only. Reply to Bill Lawry at eht-at-stlnet.com or
(314)531-9868. Thank you.


Vitaly Feingold
Scientific Instruments and Applications
(770)232-7785 ph.
(770)232-1791 fax.



From daemon Fri Mar 10 07:17:54 2000



From: Ken Tiekotter :      tiekotte-at-up.edu
Date: Fri, 10 Mar 2000 00:43:54 -0800 (PST)
Subject: Re: Objective Aperture Moving

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Peter,
I have a 7 holed, self-cleaning, gold foil aperture in my TEM. The hole
diameters are 30um and 60um. At 60kV, the aperture does tend to wander
abit until it gets warmed up. After an hour or so of operation, I
generally have to tweak the xy alignment and then I am ready to continue.

I am always amazed when the instrument is used by other investigators.
Frequently the aperture is halfway into the the beam, i.e., the crescent
of the aperture is covering 1/3 of the field of view. As I stated, I am
aware the gold foil aperture tends to drift slightly over time exposed to
the beam, and consequently adjust accordingly.

I too would be keen to hear any additional comments on this 'trait' of
gold foil apertures.

-Ken

On Thu, 9 Mar 2000 PESTOEM-at-aol.com-at-sparc5.microscopy.com wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} To All:
} We have a Jeol 100CX II and experiencing a constant moving of the Objective
} Aperture moving when scanning grids, especially in Posistion # 2 Grid.
} Any solution or ideas? We changed apertures,cleaned holder etc.
} Thank you,
} Peter Stolzenberg, PESTO INC.
} pestoem-at-aol.com
}
}



From daemon Fri Mar 10 07:17:55 2000



From: Carstensen, Jesper Vejl¿
Date: Fri, 10 Mar 2000 10:31:18 +0100
Subject: TEM: Carbides in tool steels

Contents Retrieved from Microscopy Listserver Archives
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Dear all,

I have just started on a TEM-project investigating the microstructures of
different tool steels. The steels contain various types of carbides with
different compositions, shapes and sizes. I have started out by making thin
foils using electrolytical jet polishing. I get nice thin foils where I can
study and analyse the martensitic matrix, but the carbides are off course
too thick to be analysed. To overcome this problem I have considered a
combination of electropolishing and ion milling, but I have also heard about
an extraction replication technique. However, I haven't been able to find
any literature on this technique. Have any of you tried this technique
and/or could you point out some literature describing the technique? Also,
if you have other suggestions to deal with the described problem I would be
pleased to hear about them.

Regards, Jesper

----------------------------------------------------
Jesper Vejloe Carstensen
Research Scientist, M.Sc., Ph.D.
Materials Research Department
Risoe National Laboratory
P.O. Box 49
DK-4000 Roskilde, Denmark
Phone: +45 4677 5776
Fax: +45 4677 5758
E-mail: jesper.v.carstensen-at-risoe.dk
Web: http://www.risoe.dk/AFM/Personal/Jeca/jeca.htm
----------------------------------------------------





From daemon Fri Mar 10 07:17:56 2000



From: Tim E. Harper :      tim-at-cmp-cientifica.com
Date: Fri, 10 Mar 2000 11:54:59 +0100
Subject: RE: Conference Announcement: EuroFE 2000

Contents Retrieved from Microscopy Listserver Archives
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Hi,

The url of the conference site seems to have been stripped out by some mail
servers. Just in case it's www.cmp-cientifica.com/eurofe

Regards

Tim




******************************************************************
Tim E. Harper EuroFE Network Co-Chairman
EuroFE is a network of the European Science Foundation
Phone +34 91 640 71 85 Fax +34 91 640 71 86





From daemon Fri Mar 10 21:18:58 2000



From: White, Woody N :      Woody.N.White-at-mcdermott.com
Date: Fri, 10 Mar 2000 07:58:00 -0600
Subject: Re:suggestions for new SEM??

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


One accessory worth considering, especially for a SEM used by students would
be
a ChamberScope. This is a small IR video camera mounted to the chamber
which
will allow students to see the position of samples in the chamber in real
time.
Could avoid damaging crashes. Take a look at GW Electronics for one
model....

Woody White
McDermott Technology

Me:
http://www.geocities.com/capecanaveral/3722



From daemon Fri Mar 10 21:18:59 2000



From: electron microscope laboratory :      emlab-at-udsm.ac.tz
Date: Fri, 10 Mar 2000 07:24:40 -0600
Subject: training in TEM maintenance...

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hallo,
could anybody on the net have an idea where one can attend a "training in
TEM maintenance".
Best regards,

Nyakyi




From daemon Fri Mar 10 21:19:01 2000



From: Bartek :      chmielow-at-immagene.mcg.edu
Date: Fri, 10 Mar 2000 09:02:53 -0500
Subject: 2-photon microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have Carl Zeiss LSM 510 2-photon microscope armed with a T:sapphire
laser. We are successful with one color imaging, but we wpould like to
try two-color two-photon imaging, and of course we have problems with
finding a proper set of fluorophoes. My understanding is that they
should have similar excitation and different emissions. Has anyone tried
using two or more fluorophores ? Which combinations are the best? What
wavelengths are recommended?

Bartek Chmielowski



From daemon Fri Mar 10 21:19:04 2000



From: Chengge Jiao :      c.g.jiao-at-bris.ac.uk
Date: Fri, 10 Mar 2000 14:35:09 -0000
Subject: Electron diffaction software

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Hi,

Does anybody know where I can get free software for electron doffraction and
more...

Can we download them free ?

Chengge JIAO

H.H.Wills Physics Lab
University of Bristol
c.g.jiao-at-bristol.ac.uk



From daemon Fri Mar 10 21:19:05 2000



From: rlvaughn-at-unmc.edu -at-sparc5.microscopy.com
Date: Tue, 7 Mar 2000 17:06:42 -0600
Subject: TEM - ImmunoEM reference

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Is there a short, general reference, like a review, that explains immuno
EM, it's uses, major techniques, and shortfalls? I would like to use it to
send to investigators that are thinking about using the technique but don't
know much about labeling at the EM level. I have a lot of specific
references and it would take a lot of time to reference or copy them. If
the investigator wants to know more I can direct them to those, otherwise a
general one would get them and me on the same page when we start talking
specifics on their project. Thanks

Rick Vaughn
rlvaughn-at-unmc.edu






From daemon Fri Mar 10 21:19:05 2000



From: Cap'n Peachfuzz :      bsteffen-at-arches.uga.edu
Date: Tue, 7 Mar 2000 08:48:54 -0500 (Eastern Standard Time)
Subject: Re: Microscopy of blood

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In the earlier days {25 years ago} of medical diagnosis, before the
advent of the rapid, highly specific methods of identifying pathogenic
organisms, darkfield microscopy was routinely applied to rule out
specific organisms. In particular, the diagnosis of syphilis was aided
with this mode of imaging. When fluids from a suspect lesion were
applied to a slide and imaged by darkfield, the characteristic shape of
the spirochaete organisms was clearly seen. More recently, darkfield
examination of synovial fluids from arthritic joints was used to rule
out or diagnose Lyme disease, before specific testing became available.

W. L. Steffens, Ph.D
University of Georgia





From daemon Fri Mar 10 21:19:05 2000



From: John Runions :      cjr41-at-cam.ac.uk
Date: Tue, 7 Mar 2000 11:35:10 +0100
Subject: Re: Confocal Newslist or Listserver??

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Catherine,

I also tried to subscribe to the confocal list when Larry suggested
it and it worked like a charm. I cut and pasted the email address
right from his posting.

LISTSERV-at-LISTSERV.ACSU.BUFFALO.EDU

Try again. Cheers, John


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--
C. John Runions, Ph.D.
Department of Plant Sciences
University of Cambridge
Cambridge CB4 1YE
UK

email at work cjr41-at-cam.ac.uk
email at work runions-at-ntlworld.com
Phone (01223) 766 545




From daemon Fri Mar 10 21:19:10 2000



From: Maureen Petersen :      mape-at-gnv.ifas.ufl.edu
Date: Fri, 10 Mar 2000 10:52:41 -0500
Subject: seeking flatbed scanner info

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Dear List Members:

We are in the market for two relatively inexpensive flatbed scanners. One
needs to have a USB attachment and drivers for a Mac. This one is pretty
straightforward. Up to about $200 (US) should suit our needs.

For the second one I am considering one with an adapter to allow scanning of
negatives and 35 mm slides. I have heard a bad report of a scanner not
focussing correctly on the negative or transparency. Does anyone have any
comments to support or refute the focusing problem? We are able to pay up to
$400 if I can find one that performs well. Obviously, we are not considering
high end scanners. I would prefer USB, but could cope with another type of
connection. Thank you for any help you can provide.

Sincerely,
Maureen Petersen
Dept Plant Pathology
University of FL
Gainesville, FL 32611



From daemon Fri Mar 10 21:19:10 2000



From: Wil Bigelow :      bigelow-at-engin.umich.edu
Date: Fri, 10 Mar 2000 11:30:53 -0400
Subject: RE:Moving Obj Aperture

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It has been a number of years since I've been directly involved in
operating and maintaining a JEOL 100CX, but as I recall both the specimen
holder and the aperture are inserted into the rather limited space between
the objective pole pieces, with the specimen rod fitting in above the
aperture. If the aperture consistently moves when you move the specimen
holder, then I'd strongly suspect that either the end of your specimen rod
has been bent downward just a bit, or the end of the aperture holder has
been bent upward slightly so that the specimen rod rubs the aperture holder
when it is moved thus causing the aperture to move. The clearance between
these two devices is only a fraction of a millimeter, and so it wouldn't
take much bending to cause this problem.

Good luck,

Wilbur C. Bigelow, Prof. Emeritus
Materials Sci. & Engr., University of Michigan
Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu;
Fx:734-763-4788; Ph:734-662-5237




From daemon Fri Mar 10 21:19:10 2000



From: Leah Dobbs :      s20260-at-onsemi.com
Date: Fri, 10 Mar 2000 09:31:58 -0700
Subject: Fw: Fw: Allied Multiprep Polisher for TEM samples

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} } Anita,
} } I have been using the MultiPrep for TEM of semiconductors since
December. It
} } has made my life much much easier. I am responsible for the TEM
} } preparation/imaging/data at ON Semi. Since I have started using the
MultiPrep I
} } have been able to consistently turn a job around in 1 day and less. I
have only
} } failed on 1 sample since December and that was due to my bad judgement.
Once you
} } get a routine for making the samples I have found the results to be very
} } consistent. Do you have an Ion Mill? I use the PIPS and it
contributes to
} } the success of the samples I make. Although if you did not have a PIPS
I think
} } that you would still get very good results using the MultiPrep.
} } For me the major differences between using the MultiPrep and the Tripod
Polisher
} } are:
} } 1. Consistent amount of force placed on the samples using the
MultiPrep.
} } 2. Having the digital micrometer on the MultiPrep takes some of the
guess work
} } out of the sample prep. I am able to tell how much material I am
removing
} } 3. Piece of mind. Since I have developed a set routine I am able to
make 2
} } samples a day and feel confident that they will not fail. Hand
polishing has
} } always been a crap shoot for me.
} } From your signature line I am assuming you work on various materials.
The work
} } I have done is primarily Silicon, but I think that the results would be
good for
} } other materials also. I have submitted an abstract for the MSA
conference this
} } year. I would be happy to speak with you about the system if you will
be
} } attending.
} }
} } Leah L Dobbs
} } ON Semiconductor
} } CSAL TEM
} } 602-244-4820
} } 1-888-637-9415 (6379415-at-skytel.com)
} } s20260-at-onsemi.com
} }
} } My disclaimer " I do not work for Allied High Tech, and do not have
vested
} } interest in the companies financial success. I am merely a satisfied
customer".
} }
} }
} } ----- Original Message -----
} } From: "Anita Garg" {Anita.Garg-at-lerc.nasa.gov}
} } To: {Microscopy-at-sparc5.microscopy.com}
} } Sent: Thursday, February 24, 2000 5:55 AM
} } Subject: Re: Allied Multiprep Polisher for TEM samples
} }
} }
} }
} ------------------------------------------------------------------------
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America
} } } To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
} } } On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} }
} -----------------------------------------------------------------------.
} } }
} } }
} } } Dear Colleagues
} } } Does anybody have experience on the Allied MultiPrep Polishing
} } } System, especially in terms of getting a good TEM sample? How well do
} } } the Allied's Precision Cross-Section Tool and TEM Wedge Polisher work
} } } on this semi automatic multiprep polisher?
} } } Also, how does their TEM Wedge Polisher compare with the South Bay
} } } Tripod Polisher?
} } } TIA
} } } Anita
} } } *******************************************
} } } Dr. Anita Garg
} } } NASA Glenn Research Center at Lewis Field
} } } Advanced Metallics Branch
} } } Mail Stop 49-3
} } } 21000 Brookpark Road
} } } Cleveland, OH 44135
} } } Phone : (216) 433-8908
} } } Fax : (216) 977-7132
} } } E-mail : Anita.Garg-at-grc.nasa.gov
} } } *******************************************
} } }
} } }
} }



From daemon Fri Mar 10 21:19:12 2000



From: Wil Bigelow :      bigelow-at-engin.umich.edu
Date: Fri, 10 Mar 2000 11:44:23 -0400
Subject: RE: Extraction Replicas

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The extraction replica technique was developed by Bob Fisher at the U. S.
Steel Laboratory back in the early 1950's when we were all trying to
identify the precipitates in various alloy systems for the first time. I
don't seem to be able to lay my hands on the original reference; however,
the technique is described in reasonable detail on pages 115-117 of the
book 'Techniques for Electron Microscopy', Desmond Kay, Ed., Blackwell
Scientific, 1961.

Wilbur C. Bigelow, Prof. Emeritus
Materials Sci. & Engr., University of Michigan
Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu;
Fx:734-763-4788; Ph:734-662-5237




From daemon Fri Mar 10 21:19:13 2000



From: brink-at-tiger.3dem.bioch.bcm.tmc.edu
Date: Fri, 10 Mar 2000 10:55:22 -0600 (CST)
Subject: Re: Electron diffaction software

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Hi, we have some software freely downloadable for processing ED of protein
crystals. It's at http://ncmi.bcm.tmc.edu/software.html. Look for packages
auto and edp. The former is a front-end to a bunch of fortran programs
for automated processing, whereas the latter is a GUI-based program.

Jaap

--
Jaap Brink, Ph.D., Biochemistry, One Baylor Plaza, Baylor College of
Medicine, Rm. N420 Alkek Building, Houston, TX 77030
Phone: (713)798-6989 -- Fax: (713)796-9438 -- Email: jbrink-at-bcm.tmc.edu
URL : http://ncmi.bioch.bcm.tmc.edu/~brink

On Fri, 10 Mar 2000, Chengge Jiao wrote:

} ------------------------------------------------------------------------
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} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi,
}
} Does anybody know where I can get free software for electron doffraction and
} more...
}
} Can we download them free ?
}
} Chengge JIAO
}
} H.H.Wills Physics Lab
} University of Bristol
} c.g.jiao-at-bristol.ac.uk
}
}



From daemon Fri Mar 10 21:19:18 2000



From: John J. Bozzola :      bozzola-at-siu.edu
Date: Fri, 10 Mar 2000 12:12:08 -0600
Subject: Image Analysis: NIST Particles needed

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Firstly, thanks to everyone who helped out with the earlier question
on image analysis. We are using NIH Image 1.62 (which has a watershed
filter to separate touching particles) to measure their diameters.
(Worked like a charm -- thanks John Russ).

We need a NIST particle, sized 5 µm or so, to use as a standard.
Unfortunately, the standard we recently purchased was sized using
light scattering (which apparently gives a higher reading than
electron microscopy) and our sizing does not agree with that figure.

Does anyone know were we can find 5.0 µm polystyrene particles that
have been sized by TEM and are NIST certified or traceable?

Thanks,

####################################################################
John J. Bozzola, Ph.D., Director
Micro-Imaging and Analysis Center
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
####################################################################



From daemon Fri Mar 10 21:19:21 2000



From: Tom Phillips :      PhillipsT-at-missouri.edu
Date: Fri, 10 Mar 2000 12:47:53 -0600
Subject: binucleate hepatocytes & granules in gallbladder: basic histo

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Fellow microscopists:

Every year when I teach the liver lectures for my histology course, I
explain to the students that up to 25% of the hepatocytes are
binucleate and another 50% are polyploid. These textbook facts match
up with what I see in the scope. But I don't understand why
hepatocytes (or transitional epithelial cells) are frequently
binucleate (or polyploid). Any one able to answer this structure -
function question?

My second histology question concerns the gallbladder epithelium. I
have a great prepared H&E 1.5 um paraffin slide from Carolina
Biological. Most of the epithelial cells have a few fine red-stained
granules in them. I assume these are glycoproteins and/or mucin-type
glycoproteins. A few scattered cells in the epithelium have larger,
intensely eosinophilic granules in them. I can't figure out what
these cells are. None of the standard textbooks or atlases discuss
them. Before anyone suggests they are mucin-filled goblet cells, I
should point out that intestinal goblet cells are my main research
focus and these are unlike any mucin secreting cell I have seen in
the intestine, respiratory tract, stomach, conjunctiva, etc. They
look, in fact, more like Paneth cells in the small intestine. Any
ideas?

Thanks, Tom


Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)



From daemon Fri Mar 10 21:19:25 2000



From: Cap'n Peachfuzz :      bsteffen-at-arches.uga.edu
Date: Fri, 10 Mar 2000 14:23:35 -0500 (Eastern Standard Time)
Subject: Re: Objective Aperture Moving

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It is my understanding that many instances of so-called aperture
thermal drift are actually the result of hysteresis in either the
condenser or objective lenses. This seems to be more of a problem in
JEOL instruments than in others that I'm aware of. It is particularly
pronounced when you spend time scanning large areas with little or no
changes in magnification or brightness. When the current is changed to
either lens, the periphery of an aperture may enter the field of view.
By realigning the aperture, you actually misalign it along the optical
axis. This is why a TEM is often in such abysmal alignment after use
by students and less experienced users. Our JEM 1210 has 9 lenses,
with each one potentially affected by hysteresis. Most of our users
know when it is time to switch off the lens power temporarily and
reload the alignment values. In the case of a less automated
instrument such as the 100-C series, it will often suffice to run C2,
Obj, and Int lenses through their entire ranges before aligning
apertures.



From daemon Fri Mar 10 21:19:29 2000



From: Anthony Garratt-Reed :      tonygr-at-mit.edu
Date: Fri, 10 Mar 2000 14:55:35 -0500
Subject: Re: TEM: Carbides in tool steels

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The problem with extraction replicas for your carbides is that they will
still be too thick to analyse! Extraction replicas work better the smaller
the carbides (in my humble opinion!). I would recommend mechanical
thinning followed by ion-milling (we typically don't bother with the
intermediate jet-polishing step). Of course, if you also have fine
carbides, you will need the extraction replicas in order to be able to
examine them without interference from the matrix!

Good luck,

Tony Garratt-Reed.


At 10:31 AM 03/10/2000 +0100, you wrote:
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** Anthony J. Garratt-Reed
** MIT Room # 13-1027
** 77 Massachusetts Avenue
** Cambridge, MA 02139-4307
** USA
**
** Phone: (+) 1-617-253-4622
** Fax: (+) 1-617-258-6479
**




From daemon Fri Mar 10 21:19:31 2000



From: Nancy Monteiro :      nancy_monteiro-at-ncsu.edu
Date: Fri, 10 Mar 2000 15:14:18 -0500
Subject: RE: gallbladder: basic histoquestions

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First of all you never indicated from which species this gall bladder
epithelium came from. Goblet cells are characteristic in the bovine
species. The cat epithelium may contain globule leukocytes. Also,
endocrine cells have been reported in bovine.

Nancy A. Monteiro-Riviere, Ph.D., DABFE, DABFM
Professor of Investigative Dermatology and Toxicology
North Carolina State University
College of Veterinary Medicine
Department of Clinical Sciences
Center for Cutaneous Toxicology and Residue Pharmacology
4700 Hillsborough St.
Raleigh, NC 27606
Tel: 919-513-6426
FAX: 919-513-6358
email: Nancy_Monteiro-at-ncsu.edu
CCTRP Homepage: http://cctrp.ncsu.edu

} ----------
} From: Tom Phillips[SMTP:PhillipsT-at-missouri.edu]
} Sent: Friday, March 10, 2000 1:47 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: binucleate hepatocytes & granules in gallbladder: basic
} histoquestions
}
} ----------------------------------------------------------------------
} --
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------
} -.
}
}
} Fellow microscopists:
}
} Every year when I teach the liver lectures for my histology course, I
} explain to the students that up to 25% of the hepatocytes are
} binucleate and another 50% are polyploid. These textbook facts match
} up with what I see in the scope. But I don't understand why
} hepatocytes (or transitional epithelial cells) are frequently
} binucleate (or polyploid). Any one able to answer this structure -
} function question?
}
} My second histology question concerns the gallbladder epithelium. I
} have a great prepared H&E 1.5 um paraffin slide from Carolina
} Biological. Most of the epithelial cells have a few fine red-stained
} granules in them. I assume these are glycoproteins and/or mucin-type
} glycoproteins. A few scattered cells in the epithelium have larger,
} intensely eosinophilic granules in them. I can't figure out what
} these cells are. None of the standard textbooks or atlases discuss
} them. Before anyone suggests they are mucin-filled goblet cells, I
} should point out that intestinal goblet cells are my main research
} focus and these are unlike any mucin secreting cell I have seen in
} the intestine, respiratory tract, stomach, conjunctiva, etc. They
} look, in fact, more like Paneth cells in the small intestine. Any
} ideas?
}
} Thanks, Tom
}
}
} Thomas E. Phillips, Ph.D.
} Associate Professor of Biological Sciences
} Director, Molecular Cytology Core Facility
}
} 3 Tucker Hall
} Division of Biological Sciences
} University of Missouri
} Columbia, MO 65211-7400
} (573)-882-4712 (voice)
} (573)-882-0123 (fax)
}



From daemon Fri Mar 10 21:19:34 2000



From: RCHIOVETTI-at-aol.com
Date: Fri, 10 Mar 2000 16:11:58 EST
Subject: LM: Leitz Periplan Eyepiece?

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Colleagues,

I am searching for a Leitz Periplan photomicro eyepiece, 10X/18, 23mm
diameter. The old Leitz part number was 519749. The current Leica part
number is 11519749, although it is a discontinued item. This particular
eyepiece has a threaded mount on the viewing end. The thread was used to
mount eyecups on the eyepiece, but it was also used as a photoeyepiece.

If anyone has one of these photo eyepieces and would be willing to part with
it, please contact me off-list.

TIA!

Bob Chiovetti



From daemon Fri Mar 10 21:19:46 2000



From: Soumitra Ghoshroy :      ghoshroy-at-nmsu.edu
Date: Fri, 10 Mar 2000 16:45:04 -0700
Subject: Re: immunofluorescence in methacrylate

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Your best bet is to embed in paraffin. However, if you still want to use
resin for immunolocalization, you may try Immuno-Bed Embedding resin,
available from Electron Microscopy Sciences. It is a fairly low viscosity
media.

Soumitra


} Dear microscopists,
} Can anyone offer advice on doing immunofluorescence at the light
} level? I
} have cryopreserved plant tissue that I am contemplating embedding and using
} for immunolocalization. I have a protocol that uses butyl, methyl
} methacrylate. I am wondering several things. Why butyl, methyl
} methacrylate? Are there other resins/media that are sufficient? I'd
} appreciate any advice anyone has.
} Kristen Lennon
} Kristen A. Lennon
} Cell, Molecular & Developmental Biology Group
} Department of Botany & Plant Sciences
} University of California
} Riverside, CA 92521
} kalen-at-citrus.ucr.edu




*****************************************************************
Soumitra Ghoshroy Ph.D.
Electron Microscopy Lab
Box 3EML
New Mexico State University
Las Cruces, NM 88003
Tel: 505-646-3600
Fax: 505-646-5665
e-mail: ghoshroy-at-nmsu.edu
http://confocal.nmsu.edu/eml




From daemon Fri Mar 10 21:19:47 2000



From: Gib Ahlstrand :      giba-at-puccini.cdl.umn.edu
Date: Fri, 10 Mar 2000 17:57:51 -0600 (CST)
Subject: Re: Rapid Fixation of cells for SEM)

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Robyn & others,

Here are two references more recent than the two I sent earlier regarding
simultaneous glut/osmium fixation methods for single cells in suspension:


Dentler, WL (1999) Fixation of Tetrahymena cells for electron microscopy.
Methods in Cell Biology 62:323-331

but is modified from

Omoto, C.K, and Kung, C. (1980). Rotation and twist of the central pair
microtubules in the cilia of Paramecium . J. Cell Biol 87:33-46



Responding to the message of {v03007805b4ec9d6d6eae-at-[206.69.208.21]}
from R schlicher {rkslick-at-yahoo.com} :

} Hello,
}
} I need to fix cells for viewing with SEM. However,
} the fixation method needs to be consistent with my
} experimental methods:
}
} 1) I need to fix the cells in less than 1 minute and
} due to this time limitation, I cannot spin the cells
} into a pellet and must add the cell suspension to the
} fixative; and
}
} 2)I am looking for "effects" to the cell membrane
} caused by my experiment, so I need to keep any
} possible fixative damage/effects at a minimum.
}
} I have fixed cells using plunge freezing and viewed
} them with TEM, this is time consuming and I ran into
} a
} lot of freeze damage. I would like to try chemical
} fixation and SEM. It has been suggested that I try
} glutaraldehyde + buffers, then postfix with osmium
} tetroxide and dehydrate in an ethanol series.
}
} Is this the best method? I am open to suggestions.
} I am using suspensions of prostate cancer cells in
} RPMI (+serum) growth media; I can perform the
} experiment in buffered saline and use a high
} concentration of cells.
}
} Thanks,
} Robyn
}
}
} Robyn K. Schlicher, M.S.
} Laboratory for Drug Delivery
} Institute for Bioengineering and Biosciences
} Georgia Institute of Technology
} 315 Ferst Drive
} Atlanta, GA 30332
} rkslick-at-yahoo.com

Gib Ahlstrand
Electron Optical Facility, University of Minnesota, Dept. Plant Pathology
495 Borlaug Hall, St. Paul, MN. USA. 55108 (612)625-8249
612-625-9728 FAX, giba-at-puccini.cdl.umn.edu
http://biosci.umn.edu/MIC/consortium.html



From daemon Fri Mar 10 21:19:50 2000



From: Jianguo Wen :      wenji-at-bc.edu
Date: Fri, 10 Mar 2000 19:11:40 -0500
Subject: TEM textbook

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I am going to give a course on electron microscopy including TEM, SEM, STM,
AFM, etc. to graduate students. Could you give me information on textbooks
about these microscopy.

Thanks

****************************************************
Prof. Jianguo Wen

Department of Physics
Boston College
140 Commonwealth Avenue
Chestnut Hill, MA 02467
Tel: (617) 552-3586 Fax: (617) 552-8478
Email: wenji-at-bc.edu
****************************************************



From daemon Fri Mar 10 21:19:59 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 10 Mar 2000 18:05:53 -0600
Subject: Amray 1910FE SEM maintenance contract

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Are there any Amray-trained service people out there that are
independently servicing the 1910FE system on the West Coast?
I'm in Sacramento CA. I am not looking for someone who knows
how to spell Amray. I am looking for someone who knows what
the 1910FE is all about. Big difference. Introductory questions:

1. Can you condition the emitter/gun? How and with what?
2. How do you handle the differential vacuum aperture?
3. what is your experience in working with the Windows control s/w?
4. What do you know about the IP8?
5. Do you have any knowledge about moving from 486 ISA to P-II
or P-III systems for NibbleNet and frame capture?

Since the gobbling of Amray by KLA-Tencor, Amray SEM service
is dismal--more so day by day. The service people are excellent--but they are being
pulled in all directions, and mostly towards the KLA big semi systems.

The Amray SEM systems were/are really good--and that is why KLA bought
Amray. But KLA is not a research SEM outfit like Amray was.

If I had to get something other than this 1910FE, I don't know what it
would be. The Hitachi and Philips SEMs are great/nice but ridiculously
expensive to buy and maintain. And I do not care for Jeol.

Me thinks that the research SEM options are shrinking daily.

gary g.



From daemon Fri Mar 10 21:20:00 2000



From: Marc Helvey :      Marc.Helvey-at-vlsistd.com
Date: Fri, 10 Mar 2000 18:02:23 -0800
Subject: RE: Image Analysis: NIST Particles needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dr. Bozzola -

Duke Scientific should produce something like this. I know that
they used a TEM to perform their sizing at one time. I'm not sure
if this is the still the case. If not, maybe they can point you
in the right direction. Here's Duke's website as
well as a few other particulate manufacturers:

Duke Scientific:
http://www.dukescientific.com/

Polysciences:
http://www.polysciences.com/pr/index.html

Spherotech:
http://www.spherotech.com/p0000010.htm

Seradyn:
http://www.seradyn.com/

Interfacial Dynamics:
http://www.teleport.com/~idclatex/

Bang's Labs:
http://www.bangslabs.com/

Good Luck -

================================================
Marc W. Helvey
Accounts Manager
VLSI Standards, Inc.
3087 North First Street
San Jose, CA 95134-2006
phone:(408) 428-1800, ext. 108
FAX: (408) 428-9555
Mobile: (408) 307-3833
e-mail : marc.helvey-at-vlsistd.com {mailto:marc.helvey-at-vlsistd.com}
internet: http://www.vlsistd.com {http://www.vlsistd.com/}
================================================



From daemon Sat Mar 11 10:01:11 2000



From: Nelson+ Conti :      NelsonC51-at-excite.com
Date: Fri, 10 Mar 2000 20:45:27 -0800 (PST)
Subject: Re: TEM textbook

Contents Retrieved from Microscopy Listserver Archives
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On Fri, 10 Mar 2000 19:11:40 -0500, Jianguo Wen wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I am going to give a course on electron microscopy including TEM, SEM,
STM,
} AFM, etc. to graduate students. Could you give me information on
textbooks
} about these microscopy.
}
} Thanks
}
} ****************************************************
} Prof. Jianguo Wen
}
} Department of Physics
} Boston College
} 140 Commonwealth Avenue
} Chestnut Hill, MA 02467
} Tel: (617) 552-3586 Fax: (617) 552-8478
} Email: wenji-at-bc.edu
} ****************************************************
}
}
Prof. Wen -
In 1994, I took a course on electron microscopy (for biologists) at San
Francisco State University given by Gregory Antipa (my former M.A. advisor
and principal investigator).
The textbook he used was titled
"Electron Microscopy (:) Principles and Techniques for Biologists" by John
J. Bozzola and Lonnie D. Russell (eds. Jones and Bartlett Publishers, Boston
.. London).
Although this textbook is intended for biology students, it was used in a
class that any graduate student could sign up for given his/her interests.
I found the textbook to be illuminating and interesting to read.
It has a historical chapter on electron microscopy, and then discusses,
among many topics, fixation uses / types of / .. embedding procedures and
types - TEM; similar topics - SEM; microtomy; staining procedures, types,
etc. ; TEM itself; SEM itself; immunocytochemistry and EM ; autoradiography;
freeze-fracture ; analytical electron microscopy (e..g., x-ray microanalysis
subchapter; EELS subchapter; SAD diffraction mode sub-subchapter), and many
other topics relevant to electron microscopy.
Unfortunately .. it does not cover STM (Scanning-Tunneling Microscopy) nor
AFM (Atomic Force Microscopy).
I personally do not know of any textbooks that cover those types of electron
microscopy, but I would guess that someone in this list would have
suggestions. For that matter, I am not even aware if this textbook is still
being published, but perhaps Gregory Antipa (e-mail: antipa-at-sfsu.edu ) can
tell you, or someone else.
I hope that this information may serve as a useful starting point in your
search for a textbook on electron microscopy for graduate students.
Nelson Conti (former graduate student)
San Francisco State University
1600 Holloway Avenue
San Francisco, California (CA) 94132
URL: www.sfsu.edu






_______________________________________________________
Get 100% FREE Internet Access powered by Excite
Visit http://freeworld.excite.com



From daemon Sat Mar 11 10:01:12 2000



From: Jeff & Wanda Gray :      jefwan-at-flash.net
Date: Fri, 10 Mar 2000 22:58:09 -0600
Subject: Re: hand sectioning - carbon blades

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Feather makes the blades we LM histotechs use most often in our everyday
sectioning. They are distributed by Allegiance (used to Baxter, used to be
SP....). Just FYI.
Wanda Shotsberger
(HT ASCP)
-----Original Message-----
} From: Grazyna M Tokarczyk [mailto:gmtokarc-at-is.dal.ca]
Sent: Friday, March 03, 2000 8:26 AM
To: Gordon Couger
Cc: c.jeffree-at-ed.ac.uk; Dan Luchtel; microscopy-at-sparc5.microscopy.com




On Thu, 2 Mar 2000, Gordon Couger wrote:

} ------------------------------------------------------------------------



From daemon Sat Mar 11 10:01:42 2000



From: Ken Tiekotter :      tiekotte-at-up.edu
Date: Sat, 11 Mar 2000 03:10:50 -0800 (PST)
Subject: Re: Image Analysis: NIST Particles needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear John,
I worked with characterisation of latex sphere size for many years and
traced them to NIST standard. You may wish to contact Interfacial
Dynamics Corp. as they manufacture spheres and no doubt have the capacity
to trace to NIST. I do not have their address information at hand, but
they are located in Portland, OR.

If you have other questions, please contact me at me University of
Portland email address.

-Ken





From daemon Sat Mar 11 10:01:47 2000



From: Chengge Jiao :      c.g.jiao-at-bris.ac.uk
Date: Sat, 11 Mar 2000 12:59:06 -0000
Subject: EM image simulation software

Contents Retrieved from Microscopy Listserver Archives
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Hi,

Does anybody know the price for following softwares for EM simulation.

1. Diffraction 2.0 (or the most latest version),

2. EMS.

Chengge Jiao

H.H.Wills Physics Laboratory
University of Bristol, UK

c.g.jiao-at-bristol.ac.uk



From daemon Sat Mar 11 10:01:55 2000



From: Jan Leunissen :      leunissen-at-aurion.nl
Date: Sat, 11 Mar 2000 16:28:53 +0100
Subject: Re: TEM, bio, Ag/Au enhancement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Tamara,
Without more information about the procedure that was followed it is
hard to suggest a solution. But since the issue of background with
immunogold and enhancement is becoming a regular topic in this List,
it may be worthwhile to address it more in-depth.
In troubleshooting I always find it a good idea to trace down the
origin of the problem logically, rather than shooting in the dark.
In all the years we have been involved in immunogold detection and
silver enhancement, we have always benefited from simple approaches.
These are documented in troubleshooting brochures we use during our
workshops and which describe step-by-step how to proceed to have the
best chance to pinpoint the problem. Anyone who is interested, please
send me an e-mail.

Now just some comments on some of the things you mentioned:

- working on brain slices, you are probably doing pre-embedding. One
of the issues in pre-embedding is penetration. Most of the time gold
conjugates need a longer time to get inside thicker specimens, but
under the proper conditions they do get there. But what often is
easily forgotten is that when it takes a long time for reagents to
penetrate into the slice, it also takes a long time for unreacted
reagents to get washed out again. So washing should be appropriate.

- you mentioned that there is no difference between silver
enhancement and gold enhancement. Have these specimens been incubated
with gold conjugate? What if there is background even when the gold
conjugate incubation step was left out? With any enhancement system
there is a risk of getting background through
(i) autonucleation or
(ii) by the formation of metal particles in the specimen, formed as a
result of the interaction of components in the specimen
(e.g.aldehydes, borohydrid) with the noble metal salts in the
enhancement mix.
This may also be related to the type of metal salt in the enhancement
solution: noble metal salts need only a little push to become
reduced to the metal again and the more noble the metal the smaller
the push it needs. As for judging if autonucleation has occurred, as
long as the enhancement solution is as clear as it was when the
enhancement started, this should not be a real problem.

- background from magnesium. Gallyas and Danscher (and many others)
have looked into many aspects of (auto)metallography, I can give
references if you are interested.

- light sensitivity of enhancement reagents: light sensitivity can
never be reduced to zero. There are tricks that reduce light
sensitivity to a degree where it no longer seriously interferes with
enhancement on particles, but again, these are noble metal salts, and
they need just so much....
In any case, if light would be causing a problem, which I don't think
is very likely whatever brand you used, you should have the same
phenomena as with autonucleation: the mixture should become turbid or
colored after a while. If this is not the case, there is no reason to
be worried about a possible light sensitivity.

Jan
===========================
Jan Leunissen
AURION http://www.aurion.nl
Costerweg 5
6702 AA Wageningen
phone: (31)-317-497676
fax: (31)-317-415955
You will find more tech info on our website.



From daemon Sat Mar 11 10:34:14 2000



From: yu lung chiu :      yl_chiu-at-yahoo.com
Date: Sat, 11 Mar 2000 10:03:51 -0600
Subject: Need help on PEELS

Contents Retrieved from Microscopy Listserver Archives
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Need help on PEELS
}
} To all,
}
} I am working on grain boundary chemistry measurement
} with Digipeels. Try to find out the segregation
} behavior of boron, which is about 0.5at% averagely.
} However, I cannot find any edge for boron in the
} spectrum. As I don't know what's the optimum setting
} for PEELS to check the grain boundary segregation,
if
} you have any idea on this, please let me know.
}
} Thanks,
} Lung
__________________________________________________
Do You Yahoo!?
Talk to your friends online with Yahoo! Messenger.
http://im.yahoo.com




From daemon Sat Mar 11 10:34:18 2000



From: Corneliu Sarbu :      Corneliu.Sarbu-at-mtm.kuleuven.ac.be
Date: Sat, 11 Mar 2000 10:17:35 -0600
Subject: re: TEM: Carbides in tool steels

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Dr.Carstensen,

I'd like to point out that by extraction replica you will NOT overcome the
problem of
the carbides' thickness: you will get only a complete separation of them
from matrix. So, according to my experience, the ion-milling would be a
solution in that it could lead to a THINNING of your carbides.

In case there is a possibility to partially dissolve the carbides, by chemical
means, after their extraction, then the extraction replica technique will
work;
but I don't know about such a chemical technique for dissolvind (partially!)
the carbides.

Corneliu Sarbu, Ph.D.
Dept.of Metallurgy and Materials Science
Catholic University of Leuven
Belgium

- - - - - - - - - - - - - - Original Message - - - - - - - - - - - - - -
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Dear all,

I have just started on a TEM-project investigating the microstructures of
different tool steels. The steels contain various types of carbides with
different compositions, shapes and sizes. I have started out by making thin
foils using electrolytical jet polishing. I get nice thin foils where I can
study and analyse the martensitic matrix, but the carbides are off course
too thick to be analysed. To overcome this problem I have considered a
combination of electropolishing and ion milling, but I have also heard about
an extraction replication technique. However, I haven't been able to find
any literature on this technique. Have any of you tried this technique
and/or could you point out some literature describing the technique? Also,
if you have other suggestions to deal with the described problem I would be
pleased to hear about them.

Regards, Jesper

----------------------------------------------------
Jesper Vejloe Carstensen
Research Scientist, M.Sc., Ph.D.
Materials Research Department
Risoe National Laboratory
P.O. Box 49
DK-4000 Roskilde, Denmark
Phone: +45 4677 5776
Fax: +45 4677 5758
E-mail: jesper.v.carstensen-at-risoe.dk
Web: http://www.risoe.dk/AFM/Personal/Jeca/jeca.htm
----------------------------------------------------





- - - - - - - - - - - - End of Original Message - - - - - - - - - - - -




From daemon Sat Mar 11 10:46:09 2000



From: Purdy, Sam :      SPurdy-at-nationalsteel.com
Date: Sat, 11 Mar 2000 10:30:28 -0600
Subject: RE: Jesper Carstensen/Carbides in tool steels

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Jesper:
Extraction replicas are an old technique. I used it 30 years ago.

1. Prepare a lightly etched metallographic specimen.
2. Evaporate with carbon to form a thin film.
3. If you want increased contrast, shadow with Cr at an angle.
4. Score the surface with a razor to form 1 mm rectangles.
5. Etch again until the carbon squares float off.
6. Scoop up the rectangles and immerse in water.The square may flatten
out. If so, it can be mounted on TEM grid and dried by touching the side of
the grid to a piece of filter paper.
7. If the released square curls up when immersed in the rinse water. scoop
it up with a grid and gently lower it into a bath of acetone. It should snap
out into a flat sheet floating on top of the acetone. Again mount on a grid
and dry. If this doesn't work, remove the replica from the acetone bath and
lower it into the water bath so that surface tension flattens out the
replica.

I'm surprised thnat you could not find a description of thsi
technique in the literature. Try some of the older texts on TEM techniques.

Good luck,
Sam Purdy
National Steel Corp. Technical Center
Trenton, MI, USA
} ----------
} From: "Carstensen, Jesper Vejl¿"
} Sent: March 2000 4:31 AM
} To: 'Microscopy-at-MSA.Microscopy.Com'
} Subject: TEM: Carbides in tool steels
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear all,
}
} I have just started on a TEM-project investigating the microstructures of
} different tool steels. The steels contain various types of carbides with
} different compositions, shapes and sizes. I have started out by making
} thin
} foils using electrolytical jet polishing. I get nice thin foils where I
} can
} study and analyse the martensitic matrix, but the carbides are off course
} too thick to be analysed. To overcome this problem I have considered a
} combination of electropolishing and ion milling, but I have also heard
} about
} an extraction replication technique. However, I haven't been able to find
} any literature on this technique. Have any of you tried this technique
} and/or could you point out some literature describing the technique? Also,
} if you have other suggestions to deal with the described problem I would
} be
} pleased to hear about them.
}
} Regards, Jesper
}
} ----------------------------------------------------
} Jesper Vejloe Carstensen
} Research Scientist, M.Sc., Ph.D.
} Materials Research Department
} Risoe National Laboratory
} P.O. Box 49
} DK-4000 Roskilde, Denmark
} Phone: +45 4677 5776
} Fax: +45 4677 5758
} E-mail: jesper.v.carstensen-at-risoe.dk
} Web: http://www.risoe.dk/AFM/Personal/Jeca/jeca.htm
} ----------------------------------------------------
}
}
}
}




From daemon Sun Mar 12 07:42:29 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Sat, 11 Mar 2000 12:46:08 -0600
Subject: Re: Amray 1910FE SEM maintenance contract

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 06:05 PM 3/10/00 , I wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

[snip]

Ability to spell "Amray" is/was not meant to be negative. I have had
bad experience with service people who say they can work on
systems but in reality cannot. I have replaced my Amray 1830
LaB6 system with the Amray 1910FE. The field emission system
is much more complex and horribly more expensive to replace
if not properly handled/serviced. There are special procedures
especially for the FEI 305FE Zr/W gun assembly that must be
followed or the emitter is zapped.

Bad experience was unfortunate with the LaB6 system. I fear it
would be fatal on a field emission system. That is what I was
clumsy in saying.

gary g.



From daemon Sun Mar 12 07:42:31 2000



From: Jonathan Barnard :      Jonathan.Barnard-at-Angstrom.UU.SE
Date: Sat, 11 Mar 2000 22:50:26 +0100
Subject: RE:PEELS of boron

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


First you are going to have to do a bit of reading. I know of one person
who studied the detection limits both experimentally and theoretically in
PEELS recently ( {2 years) and that is Michael Natusch (formerly Cambridge).
He found current detection limits of C in steel was only about two or three
atomic percent (four to six times higher than your boron average). Check
out Micron volume 30 (1999) pp173-183 (Natusch, Humphreys, Menon &
Krivanek) as a starting point and for references.

Even if you do see a boron peak in the spectrum you will have to work out
the signal to noise ratio (SNR) of the edge. The real problem is that you
may see something that isn't there i.e. noise, or conversely you don't see
something that you should see i.e. poor experimental execution.

To optimise the SNR you are going to need a lot of counts from the boundary
itself. One way to see if your probe is on the boundary is to look for a
possible chemical shift in the core loss edges of the matrix material since
the bonding character of the material is different at the grain boundary.

Good luck.
********************************************************
Dr Jonathan Barnard

Analytical Materials Physics
The Angstrom Laboratory, Uppsala University
P O Box 534, SE-751 21 Uppsala, Sweden
Phone: +46-(0)18-4716838 Fax: +46-(0)18-500131
http://www.angstrom.uu.se/analytical/home.html

********************************************************



From daemon Sun Mar 12 18:24:27 2000



From: S¿ren Albek :      albek-at-dorit.ihi.ku.dk
Date: Sun, 12 Dec 1999 19:29:21 +0100
Subject: Surface analysis

Contents Retrieved from Microscopy Listserver Archives
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Dear all on the Microscopy List,
I am looking for good advice concerning surface analysis of metals using
a microscope. The objects are prehistoric tools, jewellery, etc. made
out of bronze, silver or gold (or combinations).
I am interested in traces of the tools/techniques that was used on the
object. The research technique should be non-destructive. The results
will be used in archaeological research.
I am looking for suggestions about what materials to use, what
equipment is suitable/normally available and not too expensive. Is there
anybody out there who deals with these subjects in a modern, industrial
context - and who could tell me some more about, how they do it ?
References for litterature, companies, etc.

With best regards,
Mr. S. Albek



From daemon Sun Mar 12 22:16:47 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Sun, 12 Mar 2000 19:53:01 -0600
Subject: Re: Surface analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Off hand, I would think that BSE imagine with a SEM might
be useful. The difference between SE and BSE could be
revealing. Without imaging the actual specimens, I can
only speculate. But based on what you are analyzing,
SE, BSE and perhaps EDX analysis can be insightful.

gary g.



At 12:29 PM 12/12/99 , you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Mon Mar 13 08:07:05 2000



From: Witold Zielinski :      WIZIEL-at-inmat.pw.edu.pl
Date: Mon, 13 Mar 2000 08:47:55 +0000
Subject: Re: TEM: Carbides in tool steels

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


*Date sent: Fri, 10 Mar 2000 10:31:18 +0100
*From: =?iso-8859-1?Q?=22Carstensen=2C_Jesper_Vejl=F8=22?=
* {jesper.v.carstensen-at-risoe.dk}
*Subject: TEM: Carbides in tool steels
*To: "'Microscopy-at-MSA.Microscopy.Com'" {Microscopy-at-sparc5.microscopy.com}

*------------------------------------------------------------------------
*The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Please notice that extracting carbides from the matrix doesn't make
them thinner. So, it seems that your idea about ion milling is right.

Best regards and good luck,

Witold

%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
Witold Zielinski, Ph.D.
Warsaw University of Technology
Department of Materials Science and Engineering
02-507 Warszawa, Woloska 141
POLAND

phone #: /48 22/ 660 84 41
fax #: /48 22/ 848 48 75



From daemon Mon Mar 13 08:07:13 2000



From: Mark Reynolds :      efjeld-at-tiac.net
Date: Mon, 13 Mar 2000 09:00:53 -0500
Subject: RE: Amray 1910FE SEM maintenance contract

Contents Retrieved from Microscopy Listserver Archives
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Hi

We offer training in EM maintanance either through one of our "Monitoring
and Maintaining the EM" short courses or on site as required by the client.

Steve Chapman
Senior Consultant Protrain - for EM Consultancy and Training World Wide
Tel 44+ 1280 814774 Fax 44+ 1280 814007
www.emcourses.com

----- Original Message -----
} From: electron microscope laboratory {emlab-at-udsm.ac.tz}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Friday, March 10, 2000 5:24 AM


a 45 degree laser line across the sample will tell a lot about
the surface. Getting a fine enough beam would be fun.

If some one wants to try it I have a start on the code in C.

Gordon W5RED

G. C. Couger gcouger-at-couger.com Stillwater, OK
www.couger.com/gcouger
"You miss 100 percent of the shots you never take." - Wayne Gretzky



----- Original Message -----
} From: "Dr. Gary Gaugler" {gary-at-gaugler.com}
To: "Sren Albek" {albek-at-dorit.ihi.ku.dk}
Cc: "MSA listserver" {Microscopy-at-sparc5.microscopy.com}
Sent: Sunday, March 12, 2000 7:53 PM


Dear Dr. Gauler,

The E.FJELD Co. has factory trained service engineers to handle
conventional AMRAY SEM's (LaB6, tungsten). We offer routine service, spare
parts and service agreements on AMRAY SEM's throughout the country.

Your requested is tough... It is extremely difficult, regardless of
manufacturer (Hitachi, Philips, JEOL or KLA), to find experienced service
people for FE technology. We do not supply such service.

If I find a source, I will keep you posted.

Regards,

Mark Reynolds

E.FJELD Co., Inc.
3 Executive Park Drive
No. Billerica, MA 01862

TEL: (978)667-1416 x10
FAX: (978)667-9059





On Friday, March 10, 2000 7:06 PM, Dr. Gary Gaugler [SMTP:gary-at-gaugler.com]
wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Are there any Amray-trained service people out there that are
} independently servicing the 1910FE system on the West Coast?
} I'm in Sacramento CA. I am not looking for someone who knows
} how to spell Amray. I am looking for someone who knows what
} the 1910FE is all about. Big difference. Introductory questions:
}
} 1. Can you condition the emitter/gun? How and with what?
} 2. How do you handle the differential vacuum aperture?
} 3. what is your experience in working with the Windows control s/w?
} 4. What do you know about the IP8?
} 5. Do you have any knowledge about moving from 486 ISA to P-II
} or P-III systems for NibbleNet and frame capture?
}
} Since the gobbling of Amray by KLA-Tencor, Amray SEM service
} is dismal--more so day by day. The service people are excellent--but
they are being
} pulled in all directions, and mostly towards the KLA big semi systems.
}
} The Amray SEM systems were/are really good--and that is why KLA bought
} Amray. But KLA is not a research SEM outfit like Amray was.
}
} If I had to get something other than this 1910FE, I don't know what it
} would be. The Hitachi and Philips SEMs are great/nice but ridiculously
} expensive to buy and maintain. And I do not care for Jeol.
}
} Me thinks that the research SEM options are shrinking daily.
}
} gary g.
}
}



From daemon Mon Mar 13 17:01:14 2000



From: David H. Hall :      hall-at-aecom.yu.edu
Date: Mon, 13 Mar 2000 09:39:39 -0500
Subject: Re: binucleate hepatocytes & granules in gallbladder: basic

Contents Retrieved from Microscopy Listserver Archives
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Dear Tom,

I know little about liver, but if these are fairly large cells under high
demands to produce [mRNAs], the polyploidy may be a response to a
biological need. In a far simpler system, the nematode, there is good
evidence that ploidy gets regulated in direct correspondance to the
absolute size of the cell, across many different tissues, such that the
ploidy perhaps matches the needs of the cell.

see Hedgecock and White (1985) Dev Biol 107: 128-133.

Though vertebrate cells may or may not be so closely regulated, the
tendency might lie along a similar continuum?

Just a thought. Best regards,
David H. Hall
Center for C. elegans Anatomy
Department of Neuroscience
1410 Pelham Parkway
Albert Einstein College of Medicine
Bronx, NY 10461

phone (718) 430-2195 FAX (718) 430-8821
hall-at-aecom.yu.edu
website: www.aecom.yu.edu/wormem



From daemon Mon Mar 13 17:01:14 2000



From: Ric Felten :      smartech-at-javanet.com
Date: Mon, 13 Mar 2000 10:01:07 -0500
Subject: Surface analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


S¿ren,

Check out my web page for examples of the different modes of scanning
electron microscopy. It is a simple web page and has a section showing
examples of the different modes of SEM. I recently quit my job and built a
SEM lab in my basement and also have x-ray microanalysis for generating
elemental spectra on micro-volumes. I am an expert in the different SEM
contrast mechanisms, but not a metallurgist w/ any experience in the field
that you mention. SEM is non-destructive provided you can get the material
into the chamber. Some SEMs have large chambers, mine is on the smaller
side (perhaps samples {4" and somewhat flat sample would be best). I would
be glad to run 2-3 samples for free to show you what SEM can do as a
feasibility study for SEM analysis of your samples. All my output can be in
the form of jpg images so that you can send the data to experts in
archaeology and they can comment on the significance.

Good Luck

Ric Felten
www.semguy.com

-----Original Message-----
} From: S¿ren Albek [mailto:albek-at-dorit.ihi.ku.dk]
Sent: Sunday, December 12, 1999 1:29 PM
To: Microscopy-at-sparc5.microscopy.com


Dear all on the Microscopy List,
I am looking for good advice concerning surface analysis of metals using
a microscope. The objects are prehistoric tools, jewellery, etc. made
out of bronze, silver or gold (or combinations).
I am interested in traces of the tools/techniques that was used on the
object. The research technique should be non-destructive. The results
will be used in archaeological research.
I am looking for suggestions about what materials to use, what
equipment is suitable/normally available and not too expensive. Is there
anybody out there who deals with these subjects in a modern, industrial
context - and who could tell me some more about, how they do it ?
References for litterature, companies, etc.

With best regards,
Mr. S. Albek




From daemon Mon Mar 13 17:01:16 2000



From: James Roberts :      James.Roberts-at-mail.co.ventura.ca.us
Date: Mon, 13 Mar 2000 08:47:16 -0800
Subject: Re: Surface analysis

Contents Retrieved from Microscopy Listserver Archives
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In addition to the advice you have already received you might wish to consider contacting your local Forensic Science Laboratory and talk to the Firearm and Toolmark Section. What you wish to do is to determine the Class characteristics of the tools used, as you do not have the actual tool for comparison. There is also some Toolmark analysis done at certain types of machine shops but a really experienced Toolmark examiner at your local Forensic Science Lab would be much easier to locate and probably more interested in your project (I'm currently doing some work for a project on bullets from a sight, in my free evenings and I know others have in the past). Low power stereo microscopy can tell you a lot. The examiners can point you in the right direction and provide some literature sights etc., or may be interested in doing the work for you.

Jim

James L. Roberts
Firearm and Toolmark Examiner
Ventura Co. Sheriff's Lab
(805) 654-2308
James.Roberts-at-mail.co.ventura.ca.us

} } } "S¿ren Albek" {albek-at-dorit.ihi.ku.dk} 12/12/99 10:29AM } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Dear all on the Microscopy List,
I am looking for good advice concerning surface analysis of metals using
a microscope. The objects are prehistoric tools, jewellery, etc. made
out of bronze, silver or gold (or combinations).
I am interested in traces of the tools/techniques that was used on the
object. The research technique should be non-destructive. The results
will be used in archaeological research.
I am looking for suggestions about what materials to use, what
equipment is suitable/normally available and not too expensive. Is there
anybody out there who deals with these subjects in a modern, industrial
context - and who could tell me some more about, how they do it ?
References for litterature, companies, etc.

With best regards,
Mr. S. Albek






From daemon Mon Mar 13 17:01:19 2000



From: beth-at-dogwood.botany.uga.edu (Beth Richardson)
Date: Mon, 13 Mar 2000 13:28:15 -0500
Subject: double labeling

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi, I need advice on double labeling (on-grid).
I've had success doing each of the labeling experiments independently but
now the researcher wants to see double labeling. I'm doing indirect
labeling with a monoclonal antibody to fungal cell wall antigens followed
by a gold labeled secondary. In addition, I want to label the sections with
gold conjugated wheat germ agglutinin.
Should I do the antibody labeling first and then the lectin?
I've found one reference that I haven't had a chance to follow up on
(Cailliez et al, 1988) in Immunogold Silver Staining Principles, Methods,
and Applications by Hayat.
Any advice would be greatly appreciated.
Beth




From daemon Mon Mar 13 17:01:19 2000



From: Christoph Bauer :      cbauer-at-midway.uchicago.edu
Date: Mon, 13 Mar 2000 12:49:42 -0800
Subject: LowicrylK4M

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we routinely use Lowicryl K4M. We normally buy it as a kit from EM
science. They also offer a it as a MonoStep Single Component. We would
like to try it. Has anybody done the comparison between the kit and the
monostep stuff (quality, shelflive, handling, polymerisation)?
Thanks for your time,

Christoph Bauer
University of Chicago



From daemon Mon Mar 13 17:01:23 2000



From: S¿ren Albek :      albek-at-dorit.ihi.ku.dk
Date: Mon, 13 Dec 1999 22:41:22 +0100
Subject: RE:My questions

Contents Retrieved from Microscopy Listserver Archives
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Dear all,
thank you very much for all the kind answers, good ideas, and good
suggestions. It has provided me with a good range of possibilities (I am
a little overwhelmed by now :-). I must now evaluate the answers and I
will most likely return with some more questions - some of these
off-list, when appropriate.

Once again,
thank you for the interest,
and with many regards,

Mr. S.Albek



From daemon Mon Mar 13 17:01:25 2000



From: Rosemary Walsh :      rw9-at-psu.edu
Date: Mon, 13 Mar 2000 19:04:46 -0400
Subject: Quantifying the sharpness of glass knives

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers,
I have a question about the cleaved glass blades used to cut cross
sections of copolymers. Is there any way to quantify the sharpness of
the blades? Do they have a radius of curvature, or anything like that?
A client of ours has asked if the blade would be sharp enough to slice
through the hard domain (polystyrene hardness) at -20 C or -80 C,
or if it would just push it out of the way. This also applies to the
disposable blades on a cryostat used to section the same sample at
-20C prior to the cryo-ultramicrotoming. The sample is very brittle
at -120C.
Rosemary

Rosemary Walsh, Manager
The Electron Microscope Facility for the Life Sciences,
A Shared Technology Facility, The Life Sciences Consortium
1 South FrearLab
Penn State University
University Park, PA 16802
(814) 865-0212
rw9-at-psu.edu
http://www.lsc.psu.edu/stf/em/home.html




From daemon Tue Mar 14 07:09:08 2000



From: Gordon Vrololjak :      gvrdolja-at-nature.Berkeley.EDU
Date: Mon, 13 Mar 2000 15:14:21 -0800 (PST)
Subject: SEMICaps for SEM

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Hello,
We have an old semicaps 4000 for pc windows 3.1 system. I've been trying
to use it to get scale bars burned into images, but I must say that it is
very buggy. The images never burn scale bars correctly, calibration
procedures are never fully written in the manual, and often minimizing and
maximizing windows causes images to have very 'weird' color schemes.

After contacting semicaps I was told that there were no bug fixes to the
software as it was bug free. Anyone out there have some experience with
this system?
Gordon.

\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
Gordon Ante Vrdoljak Electron Microscope Lab
ICQ 23243541 http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
gvrdolja-at-nature.berkeley.edu UC Berkeley
phone (510) 642-2085 Berkeley CA 94720-3330
fax (510) 643-6207 cell (510) 290-6793



From daemon Tue Mar 14 07:09:09 2000



From: jim :      YZFRJIM-at-ix.netcom.com
Date: Mon, 13 Mar 2000 18:55:58 -0500
Subject: amray 1800 series

Contents Retrieved from Microscopy Listserver Archives
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Does anyone now anybody selling a 1800 series not field emitter, by
amray

thanks,
jim



From daemon Tue Mar 14 07:09:14 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 13 Mar 2000 17:43:13 -0600
Subject: RE: Amray 1910FE SEM maintenance contract

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


My post has generated several responses. All of which are very
helpful. Thanks to all who responded.

From following up on what I have discovered from the response from
Alex Greene, I'm rather stuck. It turns out that Amray has a lock on
the FE gun/emitter that they developed and which incorporate the
FEI W/Zr emitter (305FE). As such, no other party will support the
Amray FE systems.

There are several folks who will and do support the Amray LaB6 systems.
But none can or will support the FE systems. So for the time being,
I am stuck with Amray. Be that as it may. As I write this, Amray
(KLA-Tencor) is preparing to work on my FESEM under my existing
maintenance contract. This is good. I guess that so long as this
keeps up, I am OK. But I do expect that KLA will drop all Amray
field support in the near future. Consequently, perhaps that will
be a better time to seek alternative maintenance sources. I do
expect at this future juncture, KLA/Amray will supply parts
but will not supply warm bodies. If this is an opportunity for
independents, who knows? If you are experiencing similar
troubles, I would for one appreciate hearing about your situation.
Otherwise...

Stay tuned.

gary g.



From daemon Tue Mar 14 07:09:21 2000



From: jim :      jim-at-proscitech.com.au
Date: Tue, 14 Mar 2000 15:45:28 +1000
Subject: RE: Quantifying the sharpness of glass knives

Contents Retrieved from Microscopy Listserver Archives
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Unless somebody has done that very same thing, one cannot know. However, I am
optimistic that you could section that material with a glass knife.
A new knife should run virtually to a molecular edge, although that finest edge
would not survive the first section. The glass knife is sharp, the question
concerns more its hardness and toughness.
An LKB trainer years ago told me that a glass knife at liquid nitrogen
temperature is almost as hard as is diamond at room temperature. So there is a
good chance that glass could do. Try it.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Tuesday, March 14, 2000 9:05 AM, Rosemary Walsh [SMTP:rw9-at-psu.edu] wrote:
}
}
} Dear Listers,
} I have a question about the cleaved glass blades used to cut cross
} sections of copolymers. Is there any way to quantify the sharpness of
} the blades? Do they have a radius of curvature, or anything like that?
} A client of ours has asked if the blade would be sharp enough to slice
} through the hard domain (polystyrene hardness) at -20 C or -80 C,
} or if it would just push it out of the way. This also applies to the
} disposable blades on a cryostat used to section the same sample at
} -20C prior to the cryo-ultramicrotoming. The sample is very brittle
} at -120C.
} Rosemary
}
} Rosemary Walsh, Manager
} The Electron Microscope Facility for the Life Sciences,
} A Shared Technology Facility, The Life Sciences Consortium
} 1 South FrearLab
} Penn State University
} University Park, PA 16802
} (814) 865-0212
} rw9-at-psu.edu
} http://www.lsc.psu.edu/stf/em/home.html
}
}



From daemon Tue Mar 14 07:09:31 2000



From: J.Bruyntjes-at-voeding.tno.nl
Date: Tue, 14 Mar 2000 09:51:23 +0100
Subject: IHC on parraffin embedde tissue

Contents Retrieved from Microscopy Listserver Archives
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Dear all

We would like to do immunohistochemistry on rat lung tissue and we are
especially interrested in CD4, CD8 and some macrophage-markers such as ED1
and ED2. But we would like to have good morphology as well. I have found
some articles about IHC (rat and mice) on tissue fixed in
paraformaldehyde-lysine-peroidate and emdedded in low temp paraffin.
But most of these articles are rather old (1985-1990). Is anyone familliar
with these techniques? Is it difficult to repeat and to get similar results?


Joost Bruijntjes
TNO Nutrition and Food Research Institute
Zeist
Holland
E-mail: J.Bruyntjes-at-voeding.tno.nl





From daemon Tue Mar 14 07:09:34 2000



From: Witold Zielinski :      WIZIEL-at-inmat.pw.edu.pl
Date: Mon, 13 Mar 2000 08:47:55 +0000
Subject: Re: TEM: Carbides in tool steels

Contents Retrieved from Microscopy Listserver Archives
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Dear Andrew
In principle, sections can be orientated using any two features in
the block that run parallel to each other and normal to the knife
edge. It would be nice to have reference rods included in the block,
and there are various possible approaches to this, but I cannot see
a) a way of meeting both your criterion of providing a dimensional
reference for the assessment of shrinkage, and of orientating the
section b) a method which is generally applicable to all microtomy
techniques.

To measure shrinkage, I think I would be looking at making the
fresh specimen a standard size, following its dimensions through
processing. Two ways of making specimens a standard size occur
to me: 1) Cut slices of the tissue with razor blades mounted side-
by side separated by a spacer. Re-cut the tissue slice to make
strips with square cross section, or dice it into cubes 2) take a
tissue core with a carefully sharpened stainless steel tube
(cannula, large hypodermic needle) and follow the core diameter
through the process.

For section orientation, reference cores or rods can be melted with
a needle (wax, gelatine) or drilled (plastic) normal to the block face.
The holes may be left as voids or filled with a contrasting
embedding material (e.g. coloured wax, resin, gelatine). However
this is all a bit of a fiddle, and becomes either very difficult to
achieve or impossible in ultramicrotomy of small block faces,
ultracryotomy or cryostat sectioning with Tissue-tek or other liquid
embedment.

A general principle for section orientation and alignment that is
applicable to almost all microtomy techniques is to cut a mesa on
the block face of rhomboidal or trapezoidal shape. The mesa is cut
by removing waste with the corner of the knife (which must be
square) to a little more than the thickness of the serial sequence
you wish to cut. Since the edge-facets of the mesa will be
perfectly aligned normal to the block face the sections can be
aligned using any two corners, or if they should be lost/obscured,
by means of the surviving edges.

This may fail with whole-mounts. In this situation, usually there are
features on the periphery of the specimen that can act as reference
marks, and you still have the option to cut the block face into a
trapezoid or make a mesa, or melt holes in the embedding material
with a hot needle, etc.

Finally, a number of digital image analysis packages now offer
fourier-based image correlation for registering images when making
stereo-pairs, for example, and montages. AnalySIS is one of these:

www.soft-imaging.de


Chris

Date sent: Fri, 3 Mar 2000 09:01:49 +1300
To: c.jeffree-at-ed.ac.uk
} From: Andrew McNaughton {andrew.mcnaughton-at-stonebow.otago.ac.nz}


------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


*Date sent: Fri, 10 Mar 2000 10:31:18 +0100
*From: =?iso-8859-1?Q?=22Carstensen=2C_Jesper_Vejl=F8=22?=
* {jesper.v.carstensen-at-risoe.dk}
*Subject: TEM: Carbides in tool steels
*To: "'Microscopy-at-MSA.Microscopy.Com'" {Microscopy-at-sparc5.microscopy.com}

*------------------------------------------------------------------------
*The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Please notice that extracting carbides from the matrix doesn't make
them thinner. So, it seems that your idea about ion milling is right.

Best regards and good luck,

Witold

%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
Witold Zielinski, Ph.D.
Warsaw University of Technology
Department of Materials Science and Engineering
02-507 Warszawa, Woloska 141
POLAND

phone #: /48 22/ 660 84 41
fax #: /48 22/ 848 48 75



From daemon Tue Mar 14 18:33:08 2000



From: PESTOEM-at-aol.com
Date: Tue, 14 Mar 2000 09:04:52 EST
Subject: Aperture moving-100CX II

Contents Retrieved from Microscopy Listserver Archives
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To all,
thank you for the many suggestions regarding the moving aperture, It is
definitly not the distance between aperture & specimen holder. It was checked
several times and the clearance is there. Also, the aperture was stable
before and is now moving the most in the # 2 position.
I would appreciate any further comments.
Thank you . Peter A. Stolzenberg, Pesto Inc. " pestoem-at-aol.com"



From daemon Tue Mar 14 18:33:11 2000



From: Leroux christine :      leroux-at-univ-tln.fr
Date: Tue, 14 Mar 2000 15:45:03 +0100
Subject: TEM cross section preparation

Contents Retrieved from Microscopy Listserver Archives
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hello,
I have to prepare cross sectionning samples, and I do not know where to
buy the small
vise that I need to clamp the specimen when I have glued them (I have
already the
Mbond 610 adhesive).
I would greetly appreciate any information about this thing (I am not even
sure of the
name of it)
thank you in advance
christine

**************************************
Christine leroux
Lab. M.M.I
Universite de Toulon-Var, Bat.R
B.P.132
F-83957 La Garde Cedex
tel: 00 33 (0) 4 94 14 25 07
fax: 00 33 (0) 4 94 14 21 68

**************************************



From daemon Tue Mar 14 18:33:11 2000



From: Leona Cohen-Gould :      lcgould-at-mail.med.cornell.edu
Date: Tue, 14 Mar 2000 11:08:19 -0400
Subject: Re: IHC on parraffin embedde tissue

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



}
} Dear all
}
} We would like to do immunohistochemistry on rat lung tissue and we are
} especially interrested in CD4, CD8 and some macrophage-markers such as ED1
} and ED2. But we would like to have good morphology as well. I have found
} some articles about IHC (rat and mice) on tissue fixed in
} paraformaldehyde-lysine-peroidate and emdedded in low temp paraffin.
} But most of these articles are rather old (1985-1990). Is anyone familliar
} with these techniques? Is it difficult to repeat and to get similar results?
}
}
} Joost Bruijntjes
} TNO Nutrition and Food Research Institute
} Zeist
} Holland
} E-mail: J.Bruyntjes-at-voeding.tno.nl

**********
I have had some luck with the PLP fixation you mention (McLean & Nakane, J
Histochem Cytocem 22, 1974). I gives good structural preservation, and like
most immuno techniques, you won't know if it works with your antigen until
you try it. Are you sure you mean low temp paraffin and not resin?
Lee

Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Confocal Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175




From daemon Tue Mar 14 18:33:11 2000



From: Lehman, Ann :      Ann.Lehman-at-trincoll.edu
Date: Tue, 14 Mar 2000 09:37:07 -0500
Subject: Vibration isolation tables

Contents Retrieved from Microscopy Listserver Archives
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Dear Listers,

I'm looking for a source for vibration isolation tables for ultramicrotomy.
The marble tables that we are using now are not adequate (except during
Spring Break when no one is in the building...) I'm thinking of a system
that uses compressed gas (N2?) to float the tables.

Please respond offline (vendors welcome).

thanks, all

Ann Hein Lehman
EM Facility Mgr
Trinity College
Hartford CT
860-297-4289
860-297-2538
ann.lehman-at-trincoll.edu



From daemon Tue Mar 14 18:33:14 2000



From: Jensen, Karen :      Karen_Jensen-at-URMC.Rochester.edu
Date: Tue, 14 Mar 2000 10:53:00 -0500
Subject: Immunoelectron microscopy of myelinated nerve

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I have recently performed an immunoEM labeling procedure with
myelinated nerve embedded in Lowicryl, K4M resin. Unfortunately the myelin
is severely altered (it appears as large clealr blebs) because I am not able
to osmicate this tissue due to the UV polymerization of this resin. Does
anyone have any suggestions as to preservation of lipid laden myelin without
the use of osmium? I used methanol in the dehydration procedure which has
probably caused this artefact....

Thanks!
Karen Jensen, M.S.
Associate Scientist
Electron Microscope Research Imaging Core
University of Rochester Medical Center
Rochester, NY




From daemon Tue Mar 14 18:33:15 2000



From: Ric Felten :      smartech-at-javanet.com
Date: Tue, 14 Mar 2000 11:12:24 -0500
Subject: Confocal Microscopy in North West Ct

Contents Retrieved from Microscopy Listserver Archives
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Hello,

I lost contact w/ a certain person. All I can remember is that they were
experienced in confocal microscopy and lived in North West Ct, I think
Cornwall.

Please respond off line.

Ric
860-491-3299




From daemon Tue Mar 14 18:33:17 2000



From: Ken Converse :      qualityimages-at-netrax.net
Date: Tue, 14 Mar 2000 11:13:05 -0800
Subject: Re: Amray 1910FE SEM maintenance contract

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dr. Gary Gaugler wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} My post has generated several responses. All of which are very
} helpful. Thanks to all who responded.
}
} From following up on what I have discovered from the response from
} Alex Greene, I'm rather stuck. It turns out that Amray has a lock on
} the FE gun/emitter that they developed and which incorporate the
} FEI W/Zr emitter (305FE). As such, no other party will support the
} Amray FE systems.
}
} There are several folks who will and do support the Amray LaB6 systems.
} But none can or will support the FE systems. So for the time being,
} I am stuck with Amray. Be that as it may. As I write this, Amray
} (KLA-Tencor) is preparing to work on my FESEM under my existing
} maintenance contract. This is good. I guess that so long as this
} keeps up, I am OK. But I do expect that KLA will drop all Amray
} field support in the near future. Consequently, perhaps that will
} be a better time to seek alternative maintenance sources. I do
} expect at this future juncture, KLA/Amray will supply parts
} but will not supply warm bodies. If this is an opportunity for
} independents, who knows? If you are experiencing similar
} troubles, I would for one appreciate hearing about your situation.
} Otherwise...
}
} Stay tuned.
}
} gary g.
Gary,
Your earlier comments on how good AMRAY is really puzzle me, but be that
as it may, if any of their Federal Government customers are on the ball,
Amray will be told, as was Perkin Elmer ETEC, that they must support the
equipment for about 7 years or face lawsuits. There is an implied
responsibility when one sells expensive equipment.

Perhaps supplying parts and detailed instructions will cover them
legally, in which case we third party folks may be able to help out. If
anyone has any more detailed info on Amray service, I would love to know
because I have serviced them off and on over the years and would be
willing to help out here in the Northeast/Mid-Atlantic area.

Ken Converse
owner
Quality Images
third party SEM service
16 Creek Rd.
Delta, PA 17314

717-456-5491



From daemon Tue Mar 14 18:33:17 2000



From: Grace Kennedy :      kennedy-at-nsi.edu
Date: Tue, 14 Mar 2000 08:23:43 -0800
Subject: IHC on paraffin/CD4

Contents Retrieved from Microscopy Listserver Archives
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Have a look at: Toda, Y et al: Application of tyramide signal
amplification system to immunohistochemistry: A potent method to localize
antigens that are not detectable by ordinary method. Pathology
International 1999; 49: 479-483. They mention CD4 specifically in addition
to other antigens and outline several antigen retrieval methods.



From daemon Tue Mar 14 18:33:18 2000



From: David Henriks :      Henriks-at-CompuServe.COM
Date: Tue, 14 Mar 2000 11:36:16 -0500
Subject: TEM cross section preparation

Contents Retrieved from Microscopy Listserver Archives
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Dear Christine:

South Bay Technology, Inc. manufactures both a cross sectioning vise
(formerly produced by VCR Group) and also an XTEM clamp. Both tools are
used for the same purpose you describe although they do it a little bit
differently. I am sending you a data sheet covering each one as an
attachment to a separate e-mail. We also offer a complete cross section
sample preparation kit which provides all of the bits and pieces that you
would need to make the cross section.

I hope this helps.

Best regards-

David
Writing at 9:07:17 AM on 03/14/2000

***************************************************************************
************************

David Henriks
Vice President TEL: 800-728-2233
(toll free in the USA)
South Bay Technology, Inc. +1-949-492-2600
1120 Via Callejon FAX: +1-949-492-1499
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

***************************************************************************
************************

} } } } } Please visit us at http://www.southbaytech.com { { { { {

Manufacturers of precision sample preparation equipment and supplies for
metallography, crystallography and electron microscopy.

Message text written by Leroux christine
}
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


hello,
I have to prepare cross sectionning samples, and I do not know where to
buy the small
vise that I need to clamp the specimen when I have glued them (I have
already the
Mbond 610 adhesive).
I would greetly appreciate any information about this thing (I am not even
sure of the
name of it)
thank you in advance
christine

**************************************
Christine leroux
Lab. M.M.I
Universite de Toulon-Var, Bat.R
B.P.132
F-83957 La Garde Cedex
tel: 00 33 (0) 4 94 14 25 07
fax: 00 33 (0) 4 94 14 21 68

**************************************

{



From daemon Tue Mar 14 18:33:28 2000



From: Howell, Andy :      ahowell-at-psco.com
Date: Tue, 14 Mar 2000 11:55:10 -0700
Subject: SEM: need BSE detector

Contents Retrieved from Microscopy Listserver Archives
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I am looking for a backscattered electron detector (for elemental contrast)
to attach to a JEOL JSM-5800 SEM. This is for a university, so low-cost or
donation would be most desirable. Thanks for your help!

Andy Howell
(for the) U. of Colorado-Denver



From daemon Tue Mar 14 18:33:28 2000



From: Hao Li :      haoli-at-Glue.umd.edu
Date: Tue, 14 Mar 2000 13:51:54 -0500 (EST)
Subject: Re: TEM cross section preparation

Contents Retrieved from Microscopy Listserver Archives
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Hi, Leroux:
I think a negative tweezle will do, provided that your sample is
small. Just make sure that you do not heat
your sample too fast. Actually I designed a spring vice especially for
cross section samples, it is very good for both big and small samples, the
glue line can go thinner than 100nm easily, but
normally I do not use it since almost all of my samples are small---those
film growers are really stinge:) Anyway, if you need, I can send a
schematic to you.

Good Luck.

Hao Li

On Tue, 14 Mar 2000, Leroux christine wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} hello,
} I have to prepare cross sectionning samples, and I do not know where to
} buy the small
} vise that I need to clamp the specimen when I have glued them (I have
} already the
} Mbond 610 adhesive).
} I would greetly appreciate any information about this thing (I am not even
} sure of the
} name of it)
} thank you in advance
} christine
}
} **************************************
} Christine leroux
} Lab. M.M.I
} Universite de Toulon-Var, Bat.R
} B.P.132
} F-83957 La Garde Cedex
} tel: 00 33 (0) 4 94 14 25 07
} fax: 00 33 (0) 4 94 14 21 68
}
} **************************************
}
}




From daemon Tue Mar 14 18:33:33 2000



From: Soumitra Ghoshroy :      ghoshroy-at-nmsu.edu
Date: Tue, 14 Mar 2000 13:18:28 -0700
Subject: Toluidine blue stain

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Microscopists,

Does anyone have a protocol for making Toluidine blue stain to be used for
staining thick sections of resin embedded biological samples ?

Thanks a lot,

Soumitra



*****************************************************************
Soumitra Ghoshroy Ph.D.
Electron Microscopy Lab
Box 3EML
New Mexico State University
Las Cruces, NM 88003
Tel: 505-646-3600
Fax: 505-646-5665
e-mail: ghoshroy-at-nmsu.edu
http://confocal.nmsu.edu/eml




From daemon Tue Mar 14 18:33:33 2000



From: Soumitra Ghoshroy :      ghoshroy-at-nmsu.edu
Date: Tue, 14 Mar 2000 13:13:17 -0700
Subject: Looking for Microscope Service

Contents Retrieved from Microscopy Listserver Archives
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Dear Microscopists,

Can anyone suggest name of an individual or independent company in New
Mexico, West Texas or Arizona who can fix/service research level compound
microscopes ?

Thanks in advance,

Soumitra



*****************************************************************
Soumitra Ghoshroy Ph.D.
Electron Microscopy Lab
Box 3EML
New Mexico State University
Las Cruces, NM 88003
Tel: 505-646-3600
Fax: 505-646-5665
e-mail: ghoshroy-at-nmsu.edu
http://confocal.nmsu.edu/eml




From daemon Tue Mar 14 18:33:35 2000



From: Marisa Ahmad :      mahmad-at-semiconductor.com
Date: Tue, 14 Mar 2000 15:47:52 -0500
Subject: old PGT

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We have an old PGT System 4plus (no detector) sitting here that won't boot.
Apart from that it was working fine. If anyone would be interested in it
for parts (or whatever else you want), they are welcome to it. Not
interested in cash, just pay for your shipping and its yours. We are
located near Ottawa, Canada.

Please contact Mark Chambers at 599-6500 ext. 4269 for more info, or if you
are interested.




From daemon Tue Mar 14 18:33:39 2000



From: Diana van Driel :      dianavd-at-eye.usyd.edu.au
Date: Wed, 15 Mar 2000 08:43:45 +1100
Subject: IHC on parraffin embedde tissue

Contents Retrieved from Microscopy Listserver Archives
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Can't comment on the paraffin, but PLP certainly gives improved fixation.

Diana



Diana van Driel
Department of Clinical Ophthalmology
Sydney University
GPO Box 4337
Sydney NSW
AUSTRALIA 2001

Phone 61 2 938 27278/27395
Mob 0412 165 075
Fax 61 2 938 27318



From daemon Tue Mar 14 18:33:41 2000



From: Walck. Scott D. :      walck-at-ppg.com
Date: Tue, 14 Mar 2000 17:23:35 -0500
Subject: RE: TEM cross section preparation

Contents Retrieved from Microscopy Listserver Archives
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You can buy a nice little vise from South Bay Technology. This vise was
previously made by VCR. It can be put on a hot plate and has Teflon jaws
and a Teflon slide base so that the epoxy doesn't stick. I have two of
these. They work great. I put a small dab of epoxy on t he Teflon jaw to
determine when the epoxy is cured and scraped it off with a glass microscope
slide. SBT also has two spring loaded clamping devices that can be used,
but I recommend the small vise. You can also buy a spring loaded clamping
device from Fischione and from Gatan.
-Scott



Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)


"The opinions expressed are those of Scott D. Walck and not of PPG
Industries, Inc. nor of any PPG-associated companies."
--



} -----Original Message-----
} From: Leroux christine [mailto:leroux-at-univ-tln.fr]
} Sent: Tuesday, March 14, 2000 9:45 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: TEM cross section preparation
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.} html
}
}
} --------------------------------------------------------------
} ---------.
}
}
} hello,
} I have to prepare cross sectionning samples, and I do not
} know where to
} buy the small
} vise that I need to clamp the specimen when I have glued
} them (I have
} already the
} Mbond 610 adhesive).
} I would greetly appreciate any information about this thing
} (I am not even
} sure of the
} name of it)
} thank you in advance
} christine
}
} **************************************
} Christine leroux
} Lab. M.M.I
} Universite de Toulon-Var, Bat.R
} B.P.132
} F-83957 La Garde Cedex
} tel: 00 33 (0) 4 94 14 25 07
} fax: 00 33 (0) 4 94 14 21 68
}
} **************************************
}
}



From daemon Tue Mar 14 18:33:41 2000



From: Walck. Scott D. :      walck-at-ppg.com
Date: Tue, 14 Mar 2000 17:27:03 -0500
Subject: RE: Vibration isolation tables

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Here is the web site for TMC: http://www.techmfg.com/vibrasolutions.html
and http://www.techmfg.com/65floorplatfrm.html
-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)



} -----Original Message-----
} From: Lehman, Ann [mailto:Ann.Lehman-at-trincoll.edu]
} Sent: Tuesday, March 14, 2000 9:37 AM
} To: 'MSA Listserver'
} Subject: Vibration isolation tables
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} ---------.
}
}
} Dear Listers,
}
} I'm looking for a source for vibration isolation tables for
} ultramicrotomy.
} The marble tables that we are using now are not adequate
} (except during
} Spring Break when no one is in the building...) I'm thinking
} of a system
} that uses compressed gas (N2?) to float the tables.
}
} Please respond offline (vendors welcome).
}
} thanks, all
}
} Ann Hein Lehman
} EM Facility Mgr
} Trinity College
} Hartford CT
} 860-297-4289
} 860-297-2538
} ann.lehman-at-trincoll.edu
}



From daemon Tue Mar 14 18:33:43 2000



From: Elaine Humphrey :      ech-at-unixg.ubc.ca
Date: Tue, 14 Mar 2000 14:53:42 -0800
Subject: Re: Toluidine blue stain

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} Dear Microscopists,
}
} Does anyone have a protocol for making Toluidine blue stain to be used for
} staining thick sections of resin embedded biological samples ?
}
} Thanks a lot,
}
} Soumitra
}
}


Toludine Blue Stain

100g Toludine Blue
100g Borax (sodium borate)
1000 mls ddw.

Older stain works better than newer. Filter before use.


Dr. Elaine Humphrey
Biosciences Electron Microscopy Facility
University of British Columbia
6270 University Blvd, mail-stop Botany
Vancouver, BC
CANADA, V6T 1Z4
Phone: 604-822-3354
FAX: 604-822-6089
e-mail: ech-at-unixg.ubc.ca




From daemon Tue Mar 14 18:33:43 2000



From: Huggins, Bradley J :      HUGGINBJ-at-BP.com
Date: Tue, 14 Mar 2000 16:53:44 -0600
Subject: Cryo-microscopy of solid mineral oils

Contents Retrieved from Microscopy Listserver Archives
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Hello all,
We are trying to get a project started that involves imaging the
microstructure of mineral oils and polyalphaolefins as they solidify. The
solid temperature range for materials like these is approximately -20 to -70
C. Does anyone have experience with these or similar materials. We are
hoping to image the particle structure (crystals) by SEM. Any feedback from
those with similar projects/experiences would be very welcome (on or off the
list). Even better would be references to any published reports of this
kind of material exam.

Thanks,
Brad Huggins
BPAmoco,
Electron Microscopy Lab
hugginbj-at-bp.com



From daemon Tue Mar 14 18:33:45 2000



From: Diana_Papoulias-at-usgs.gov (Diana Papoulias)
Date: Tue, 14 Mar 2000 17:03:10 -0700
Subject: stockard's solution

Contents Retrieved from Microscopy Listserver Archives
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Could someone please send me the recipe for preparing Stockard's
solution?

Thank you,

Diana Papoulias
Fisheries Biologist, Research
Columbia Environmental Research Center
US Geological Survey
4200 New Haven Rd.
Columbia, MO 65201

T:573 876 1902
F:573 876 1896
E:Diana_Papoulias-at-usgs.gov




From daemon Tue Mar 14 18:33:45 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Tue, 14 Mar 2000 15:16:17 -0600
Subject: Re: Amray 1910FE SEM maintenance contract

Contents Retrieved from Microscopy Listserver Archives
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At 01:13 PM 3/14/00 , you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

[snip]


} Gary,
} Your earlier comments on how good AMRAY is really puzzle me, but be that
} as it may, if any of their Federal Government customers are on the ball,
} Amray will be told, as was Perkin Elmer ETEC, that they must support the
} equipment for about 7 years or face lawsuits. There is an implied
} responsibility when one sells expensive equipment.
}
} Perhaps supplying parts and detailed instructions will cover them
} legally, in which case we third party folks may be able to help out. If
} anyone has any more detailed info on Amray service, I would love to know
} because I have serviced them off and on over the years and would be
} willing to help out here in the Northeast/Mid-Atlantic area.
}
} Ken Converse
} owner
} Quality Images
} third party SEM service
} 16 Creek Rd.
} Delta, PA 17314
}
} 717-456-5491

I take it that you don't think that Amrays are very good. What aspect
of them do you find lacking? I would tend to agree that the early models
are no comparison to their generations of the last 6-10 years. The FE
systems are particularly good. My 1910FE does a great job being an
FE and having the flat lens. The 1800 series with conical lenses are
not as good, but are required when doing IC wafers.

You have a great point about US Gov users. I know several of them.
When does the 7 years start? From what event or timepoint?
Amray has not stopped providing service. But the word is that they
most likely will in about 2 years....based on their takeover by KLA-Tencor.

One fellow pointed out the proprietary nature of the FE gun. Without
access to this, third party providers cannot work on the systems....at
least not on the gun assembly.

tnx,
gary



From daemon Tue Mar 14 18:33:46 2000



From: Huggins, Bradley J :      HUGGINBJ-at-BP.com
Date: Tue, 14 Mar 2000 17:30:41 -0600
Subject: Polymer imaging - PET microstructure

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Hello again Listers!

My second topic for the day is FESEM or TEM imaging of the
crystalline/amorphous microstructure of poly(ethylene terephthalate), PET.
I have on many occasions had success achieving minimal contrast of such
domains in certain unstained PET polymers in the SEM and FESEM. Recently we
have "almost" been able to observe the crystalline domains with enough
clarity to size them, this by direct examination of polished (microtomed)
surfaces. I would like to pursue the microstructural characterization a
step further by using staining or etching techniques (or some other trick
that some one may have up their sleeve!). In Polymer Microscopy (L
Sawyer, D Grubb) aminolysis appears to be (cautiously) recommended.
Staining with PTA is also mentioned. I am looking to image crystalline
domains in the range of approximately 50 to 150 nm.

Does anybody on the List have first hand experience with these or other
techniques to enhance the direct (or indirect) observation of these domains
in PET. I'm not looking to re-invent the wheel here, so any help will be
greatly appreciated. Get back to me direct or on the List.

Again,
Thanks in advance,
Brad Huggins
BPAmoco,
Electron Microscopy Lab
hugginbj-at-bp.com



From daemon Tue Mar 14 18:33:46 2000



From: Caroline Schooley :      schooley-at-mcn.org
Date: Tue, 14 Mar 2000 15:55:52 -0800 (PST)
Subject: Re: TEM cross section preparation

Contents Retrieved from Microscopy Listserver Archives
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} hello,
} I have to prepare cross sectionning samples, and I do not know where to
} buy the small
} vise that I need to clamp the specimen when I have glued them (I have
} already the
} Mbond 610 adhesive).
} christine

You haven't told us what equipment you will use to prepare your "cross
sectionning samples", so it's hard to provide advice!


Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/PCI/pci.html




From daemon Tue Mar 14 18:33:47 2000



From: G. Macdonald :      glenmac-at-u.washington.edu
Date: Tue, 14 Mar 2000 16:12:13 -0800 (PST)
Subject: Re: Toluidine blue stain

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I would suspect Elaine is giving a 10X stock solution?

We use 1% Toluidine Blue dissolved in 1% sodium borate. Filter before use.
Diluting 10-fold
with 1% sodium borate, to 0.1% toluidine blue, allows lightly
counterstaining sections developed for autoradiography.

Refs.
Mercer, E.H., J Royal Microscopy Society, 81:179 (1963)
Burns, W.A., "Thick Sections: Technique and Applications", Diagnostic
Electron Microscopy, Chapter 4, B.F. Trump and R.J. Jones, eds., John
Wiley and Sons, New Yourk , 1978.

Regards,
Glen


Glen MacDonald
Research Scientist
Hearing Research Laboratories of the
Virginia Merrill Bloedel Hearing Research Center
Box 35-7923
University of Washington
Seattle, WA 98195-7923
(206) 616-4156
glenmac-at-u.washington.edu

} } Dear Microscopists,
} }
} } Does anyone have a protocol for making Toluidine blue stain to be used for
} } staining thick sections of resin embedded biological samples ?
} }
} } Thanks a lot,
} }
} } Soumitra
} }
} }
}
}
} Toludine Blue Stain
}
} 100g Toludine Blue
} 100g Borax (sodium borate)
} 1000 mls ddw.
}
} Older stain works better than newer. Filter before use.
}
}
} Dr. Elaine Humphrey
} Biosciences Electron Microscopy Facility
} University of British Columbia
} 6270 University Blvd, mail-stop Botany
} Vancouver, BC
} CANADA, V6T 1Z4
} Phone: 604-822-3354
} FAX: 604-822-6089
} e-mail: ech-at-unixg.ubc.ca
}
}
}
}



From daemon Tue Mar 14 18:33:47 2000



From: =shAf= :      mshaf-at-darkwing.uoregon.edu
Date: Tue, 14 Mar 2000 16:15:47 -0800
Subject: RE: SEMICaps for SEM

Contents Retrieved from Microscopy Listserver Archives
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Gordon writes ...

} We have an old semicaps 4000 for pc windows 3.1 system.
} I've been trying to use it to get scale bars burned
} into images, but I must say that it is very buggy. ...

First, I need to admit no experience with this system ... but even
with my software which does produce accurate scale bars, I still
prefer to annotate the images with an image editor like Photoshop.

If you have a magnification standard, then you should be able to
determine an instrumental constant which can be assumed doesn't change
with magnification, but may change with the SEM's working distance.
You should be able to end up with this equation:

microns per pixel = constant/magnification

This may need to be determined for each fixed working distance, or
you may be able to determine how to incorporate WD into the equation
(my own software compensates with the objective lens setting so the
mag is relatively accurate). If your image acquisition options
include pixel dimentions (e.g., 256x256, 512x512, 1024x1024), then
this needs to be a factor as well (in the denominator).

I then created a spreadsheet for the pixel dimentions of common scale
bars for different magnifications, printed it, and then made it
available at the Photoshop workstation.

I can provide an example of the spreadsheet to anyone interested.

cheerios, shAf

{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {}
Michael Shaffer, R.A. - ICQ 210524
Geological Science's Electron Probe Facility - University of Oregon
mshaf-at-darkwing.uoregon.edu - http://darkwing.uoregon.edu/~mshaf/





From daemon Tue Mar 14 18:33:48 2000



From: Elaine Humphrey :      ech-at-unixg.ubc.ca
Date: Tue, 14 Mar 2000 16:19:14 -0800
Subject: Re: Toluidine blue stain

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thanks Glen, you are quite right. It is easier to make up 10g Toluidine
Blue, 10g sodium borate and 1000mls water.
Elaine

} I would suspect Elaine is giving a 10X stock solution?
}
} We use 1% Toluidine Blue dissolved in 1% sodium borate. Filter before use.
} Diluting 10-fold
} with 1% sodium borate, to 0.1% toluidine blue, allows lightly
} counterstaining sections developed for autoradiography.
}
} Refs.
} Mercer, E.H., J Royal Microscopy Society, 81:179 (1963)
} Burns, W.A., "Thick Sections: Technique and Applications", Diagnostic
} Electron Microscopy, Chapter 4, B.F. Trump and R.J. Jones, eds., John
} Wiley and Sons, New Yourk , 1978.
}
} Regards,
} Glen
}
}
} Glen MacDonald
} Research Scientist
} Hearing Research Laboratories of the
} Virginia Merrill Bloedel Hearing Research Center
} Box 35-7923
} University of Washington
} Seattle, WA 98195-7923
} (206) 616-4156
} glenmac-at-u.washington.edu
}
} } } Dear Microscopists,
} } }
} } } Does anyone have a protocol for making Toluidine blue stain to be used for
} } } staining thick sections of resin embedded biological samples ?
} } }
} } } Thanks a lot,
} } }
} } } Soumitra
} } }
} } }
} }
} }
} } Toludine Blue Stain
} }
} } 100g Toludine Blue
} } 100g Borax (sodium borate)
} } 1000 mls ddw.
} }
} } Older stain works better than newer. Filter before use.
} }

Dr. Elaine Humphrey
Biosciences Electron Microscopy Facility
University of British Columbia
6270 University Blvd, mail-stop Botany
Vancouver, BC
CANADA, V6T 1Z4
Phone: 604-822-3354
FAX: 604-822-6089
e-mail: ech-at-unixg.ubc.ca




From daemon Tue Mar 14 18:44:00 2000



From: Ben Craft :      bcraft-at-uci.edu
Date: Tue, 14 Mar 2000 16:36:44 -0800 (PST)
Subject: Best way to maintain SEM/TEM facilities

Contents Retrieved from Microscopy Listserver Archives
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We have a FEI CM20 (TEM) and XL30 (FEGSEM) and are in the process of
reviewing service contracts vs insurance. The service contract runs a min
of 40K for both instruments. We currently have a tech that can do minor
service, yearly maintenance, and alignments.

I'd appreciate any input as to how other organizations have dealt with
this.

Thank you,

Ben Craft





From daemon Tue Mar 14 20:31:31 2000



From: A.P. Alves de Matos :      apmatos-at-ip.pt
Date: Tue, 14 Mar 2000 19:27:24 -0600
Subject: TEM: LR-White polimerization

Contents Retrieved from Microscopy Listserver Archives
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}
} Dear Collegues
}
} I'm having a problem with LR-White polimerization.
}
} I use to polimerise the LR-white resin at 60¼C without any aditives and it
} usually works without problems. A new batch of the resin failed to
} polimerise, and the manufacturer claims that it is necessary to use the
} catalyst.
}
} I understand that the "accelerator" should not be used except for cold
cure,
} and even then may be harmfull since the specimen may be subject to too
much
} heat even under those conditions.
}
} I would like your opinion on:
} 1. is it necessary to use the catalyst for heat cure?
}
} 2. If it is, is there any difference among the resins that may justify the
} apparent success of the old protocol (good cure without catalyst?)
}
} Thanks in advance
}
} Dr. A.P. Alves de Matos
}
}
}




From daemon Tue Mar 14 20:31:34 2000



From: Meyers, Greg (GF) :      GFMEYERS-at-dow.com
Date: Tue, 14 Mar 2000 20:43:41 -0500
Subject: Scanned Probe Microscopist Position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Position: SCANNED PROBE MICROSCOPIST

Full-time
The Dow Chemical Company, Midland, Michigan with possible relocation to
Dow's Freeport, Texas facility
Equal opportunity employer offering a competitive compensation and benefits
package including 401k, stock purchase, performance incentives, and
educational assistance

Responsibilities:
Support existing businesses using SPM and develop new customers for SPM as
appropriate.
Calibrate and maintain SPM instrumentation base consisting of Digital
Instruments' Multimode SPM, D3000 SPM, Hysitron Nanoindentation and TA
Instruments Micro-TA SThM.
Participate in business aligned, original research programs through internal
development funding.
Bring in new technology through previous experience or external contacts as
appropriate.

Qualifications:
MS degree in Polymer Science, Materials Science or Chemistry. Ph.D. degree
is preferred.
Experience in scanning probe microscopy characterization of polymeric
materials.
Experience with Digital Instruments Nanoscope is desired.
Routine experience with a variety of imaging modes (e.g. TappingMode, phase,
friction) as well as unscanned modes (force curve, nanoindentation) is also
desired.
Leading research in the polymer SPM area.


Send Resumes by April 10, 2000 to:
The Dow Chemical Company, P. O Box 1655,
Workforce Planning Department Job 00-94/MLK, Midland, Michigan, United
States, 48641-1655 or e-mail: R&D-at-dow.com. Email respondents must list Job
00-94/MLK and their last name as the first and second items on the Subject
line. Only those selected for an interview will be contacted.

________________________________________________________________________
The Dow Chemical Company is a global science and technology based company
that develops and manufactures a portfolio of chemical, plastic and
agricultural products and services for customers in 168 countries around the
world. With annual sales of more than US$18 billion, Dow conducts its
operations through 14 global businesses employing 39,000 people. The
company has 123 manufacturing sites in 32 countries and supplies more than
3,500 products. The 39,000 Dow people around the world develop solutions
for society based on Dow's inherent strength in science and technology -
which we refer to as "good thinking." Good thinking helps customers
succeed, stockholders prosper, employees achieve and communities thrive.
For more news and information about Dow, please visit our web site at
www.dow.com.
________________________________________________________________________


Gregory F. Meyers, Ph.D.
The Dow Chemical Company
Analytical Sciences
1897E Building
Midland, MI 48667
phone: 517-636-4149
FAX: 517-638-6443
e-mail: gfmeyers-at-dow.com




From daemon Tue Mar 14 20:31:35 2000



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Tue, 14 Mar 2000 18:18:00 -0800
Subject: Toluidine blue stain

Contents Retrieved from Microscopy Listserver Archives
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1% Toluidine Blue in 1x PBS pH 7.5:

Weight 0.5 g Tolyidine Blue in some bottle (I am using 50 ml polypropylene
tissue culture tubes), add 50 ml 1x PBS and heat it in boiled water for
20-30 min. When hot, filter it using Whatman filter paper (Cat No 1001 090).

Staining:
Plastic sections up to 0.5 microns thick on the microscope slide cover with
several drops of the stain and heat on heat-plate, 60oC for 5-10 min. Wash
away stain with tap water, wash in distilled water, dry and enjoy the view.

Godd luck, Sergey.


} Date: Tue, 14 Mar 2000 13:18:28 -0700
} From: Soumitra Ghoshroy {ghoshroy-at-nmsu.edu}
} Subject: Toluidine blue stain
} X-Sender: ghoshroy-at-cnmailsvr.nmsu.edu
} To: Microscopy-at-sparc5.microscopy.com
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu
http://www.bol.ucla.edu/~sryazant




From daemon Tue Mar 14 20:47:59 2000



From: Ben Craft :      bcraft-at-mercury.oac.uci.edu
Date: Tue, 14 Mar 2000 18:43:58 -0800 (PST)
Subject: Best way to maintain SEM/TEM facilities

Contents Retrieved from Microscopy Listserver Archives
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We have a FEI CM20 (TEM) and XL30 (FEGSEM) and are in the process of
reviewing service contracts vs insurance. The service contract runs a min
of 40K for both instruments. We currently have a tech that can do minor
service, yearly maintenance, and alignments. We have not had a service
contract for the last 2 years and have had no problem getting FEI to come
out ASAP when needed.

I'd appreciate any input as to how other organizations have dealt with
this.

Thank you,

Ben Craft




From daemon Wed Mar 15 02:30:45 2000



From: A. P. Alves de Matos :      apmatos-at-ip.pt
Date: Qua, 15 Mar 2000 08:22:48 +0000
Subject: RE: Aperture moving Jeol

Contents Retrieved from Microscopy Listserver Archives
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I have a (possibly) similar problem with JEOL 100SX microscope.
Very often the beam keeps tilting in a motion that displaces it relative to the objective aperture. The effect is
the same as moving the aperture.
I suspect of circuit instability but the assistance was unable to find any trouble in that area.
Another possibility is charge accumulation inducing electric fields and displacing the beam. Since you are
lucki to have the problem mostly in one position, I would suspect of some kind of electric isolation of the
aperture holder either in that hole or produced when the entire piece goes into that position.
The charge effects could be also related to the specimen itself. Jeol seems to be particularly sensitive to this.
If you are looking at epon sections try to coat them with a thin layer of carbon (after staining, evaporate directly
into the sections) - it gives much improvement for me.

Dr. A.P. Alves de Matos



To all,
thank you for the many suggestions regarding the moving aperture, It is
definitly not the distance between aperture & specimen holder. It was checked
several times and the clearance is there. Also, the aperture was stable
before and is now moving the most in the # 2 position.
I would appreciate any further comments.
Thank you . Peter A. Stolzenberg, Pesto Inc. " pestoem-at-aol.com"






From daemon Wed Mar 15 02:40:43 2000



From: jim :      jim-at-proscitech.com.au
Date: Wed, 15 Mar 2000 18:32:59 +1000
Subject: RE: LR-White polimerization

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Most LR White is sold with catalyst already added. This will cure at 60 degrees
and must be stored refrigerated. If you purchased uncatalysed (the only way we
supply LR White because we are a long way from the UK) then prior to first use
the catalyst must be added to the whole bottle and well mixed to dissolve.
Thereafter store the catalysed LR White refrigerated.

If you want to cold cure you need to additionally add accelerator. Accelerator
is not supplied with a bottle of LR White, but when you purchase a "kit".
Instruction notes are linked online from the LR White.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Wednesday, March 15, 2000 11:27 AM, A.P. Alves de Matos [SMTP:apmatos-at-ip.pt]
wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} }
} } Dear Collegues
} }
} } I'm having a problem with LR-White polimerization.
} }
} } I use to polimerise the LR-white resin at 60oC without any aditives and it
} } usually works without problems. A new batch of the resin failed to
} } polimerise, and the manufacturer claims that it is necessary to use the
} } catalyst.
} }
} } I understand that the "accelerator" should not be used except for cold
} cure,
} } and even then may be harmfull since the specimen may be subject to too
} much
} } heat even under those conditions.
} }
} } I would like your opinion on:
} } 1. is it necessary to use the catalyst for heat cure?
} }
} } 2. If it is, is there any difference among the resins that may justify the
} } apparent success of the old protocol (good cure without catalyst?)
} }
} } Thanks in advance
} }
} } Dr. A.P. Alves de Matos
} }
} }
} }
}
}



From daemon Wed Mar 15 04:10:30 2000



From: Jonathan Barnard :      Jonathan.Barnard-at-Angstrom.UU.SE
Date: Wed, 15 Mar 2000 10:51:10 +0100
Subject: TEM: moving apertures on 100CX II

Contents Retrieved from Microscopy Listserver Archives
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I do not know what the objective aperture strip looks like on a 100 CX so I
am sort of guessing.

I have seen some aperture rods where the end consists of say three or four
holes of identical diameter and small Pt/Mo discs with different sized
holes etched in.
If the No2 aperture is moving the most then this could possibly explain it.
This disc maybe more loosely held or has a worse connection to ground than
the others. Can you check these with a multimeter or something?

What does the movement look like:- continuous slow/ slow with fast jumps/
rapid shaking/random direction/ one direction: along the rod or side to
side? And does the total beam current affect the motion greatly?

********************************************************
Dr Jonathan Barnard

Analytical Materials Physics
The Angstrom Laboratory, Uppsala University
P O Box 534, SE-751 21 Uppsala, Sweden
Phone: +46-(0)18-4716838 Fax: +46-(0)18-500131
http://www.angstrom.uu.se/analytical/home.html

********************************************************



From daemon Wed Mar 15 04:20:29 2000



From: Chris Jeffree :      cjeffree-at-srv0.bio.ed.ac.uk
Date: Wed, 15 Mar 2000 10:15:57 +0000
Subject: Non-shrinking resin

Contents Retrieved from Microscopy Listserver Archives
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Dear All

I need to embed some complex glass mouldings which also
contain components made of plastics (HDPE, PP, PC) in (for
preference) clear resin for sectioning with a diamond saw. This has
turned out to be much more problematical than I had expected, for
two reasons:

1) Very considerable shrinkage of the resins occurs during curing
(applies to acrylic, epoxy or polyester types) . This causes
separation of the resin from the glass and plastics components
inside partially-enclosed voids, so that the original spatial
relationships are lost.

2) bonding of the resins to the glass is quite variable, but typically
poor. Bonding to plastics is poor. When the bond-strength to glass
is high, particularly with epoxies, the stresses induced by the
shrinking resin during curing are large enough to fracture the glass.

Is there such a thing as a non-shrinking resin?
How can I improve bond-strength between glass and resin?
How can I improve bond-strength between resin and HDPE or PP?

Any tips gratefully received
Yours
Chris
=====================================================================
DR CHRIS JEFFREE
BIOSEM - BIOLOGICAL SCIENCES EM FACILITY
UNIVERSITY OF EDINBURGH
Daniel Rutherford Building
King's Buildings, Mayfield Road
EDINBURGH, EH9 3JH, Scotland, UK
Tel. #44 131 650 5345
FAX. #44 131 650 6563
Mobile 0410 585 401
email c.jeffree-at-ed.ac.uk
SEM / TEM bookings sem-at-ed.ac.uk
=====================================================================



From daemon Wed Mar 15 04:30:27 2000



From: John :      cjr41-at-cam.ac.uk
Date: Wed, 15 Mar 2000 10:18:38 +0000
Subject: Re: Toluidine blue stain

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Soumitra,

To stain resin, e.g. Spurr's, toluidine blue must be prepared at high pH.

0.5% toluidine blue in 0.1%sodium carbonate (Na2CO3) (pH 11.1) works well.
Stain sections on slides on a hot plate at 60C for 30sec. to 2 min. and rinse
with water. Some resins and plastics are much easier to stain than Spurr's,
e.g. JB-4, GMA etc. so I also use 0.1% tol blue in 0.01% sodium carbonate (pH
6.6). The basic protocol stays the same, you vary concentrations to adjust
pH and therefore staining intensity. Hope this helps, John.

Soumitra Ghoshroy wrote:

} Dear Microscopists,
}
} Does anyone have a protocol for making Toluidine blue stain to be used for
} staining thick sections of resin embedded biological samples ?
}
} Thanks a lot,
}
} Soumitra
}
} *****************************************************************
} Soumitra Ghoshroy Ph.D.
} Electron Microscopy Lab
} Box 3EML
} New Mexico State University
} Las Cruces, NM 88003
} Tel: 505-646-3600
} Fax: 505-646-5665
} e-mail: ghoshroy-at-nmsu.edu
} http://confocal.nmsu.edu/eml

--
C. John Runions, Ph.D.
Department of Plant Sciences
University of Cambridge
Downing St.
Cambridge
CB2 3EA
UK

email cjr41-at-cam.ac.uk
phone (01223) 766 545




From daemon Wed Mar 15 04:30:27 2000



From: Ron Doole :      ron.doole-at-materials.oxford.ac.uk
Date: Wed, 15 Mar 2000 10:03:14 +0000 (GMT)
Subject: Re: Aperture moving-100CX II

Contents Retrieved from Microscopy Listserver Archives
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Hi Peter,

From your comments I would suspect that you have a charging
problem. As it seems to be dependent on the aperture chosen it may be on
the aperture mechanism. Try cleaning it, check that there is electrical
continuity along the rod and that the mechanism is properly grounded. Do
you have a touch switch? If so then ensure that it is grounded
through the resistor properly.

Good luck,
Ron


On Tue, 14 Mar 2000 PESTOEM-at-aol.com-at-sparc5.microscopy.com wrote:}

} thank you for the many suggestions regarding the moving aperture, It is
} definitly not the distance between aperture & specimen holder. It was checked
} several times and the clearance is there. Also, the aperture was stable
} before and is now moving the most in the # 2 position.
} I would appreciate any further comments.
} Thank you . Peter A. Stolzenberg, Pesto Inc. " pestoem-at-aol.com"
}
}

===========================================================================
Mr. Ron Doole e-mail ron.doole-at-materials.ox.ac.uk
Department of Materials, phone +44 (0) 1865 273701
University of Oxford, fax +44 (0) 1865 283333
Parks Road.
Oxford. OX1 3PH. UK.
============================================================================



From daemon Wed Mar 15 06:50:06 2000



From: Dr P. Echlin :      pe13-at-cus.cam.ac.uk
Date: Wed, 15 Mar 2000 12:43:55 +0000 (GMT)
Subject: Re: Cryo-microscopy of solid mineral oils

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Bradley:

I know oil based fuels have been imaged at low temperature. Suggest you
read (better still, Buy) my book "Low Temperature Microscopy and
Analysis" Plenum Press New York 1992. I would modestly suggest it has a
lot of information about how to prepare, observe and analyse specimens
at low temperature. My own current interests revolve around low volyage,
low temperature x-ray microabalysis of bio-organic materials. Let me
know if I can be of any further help

Patrick Echlin
University of Cambridge UK

pe13-at-cus.cam.ac.uk


On Tue, 14 Mar 2000, Huggins, Bradley
J wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hello all,
} We are trying to get a project started that involves imaging the
} microstructure of mineral oils and polyalphaolefins as they solidify. The
} solid temperature range for materials like these is approximately -20 to -70
} C. Does anyone have experience with these or similar materials. We are
} hoping to image the particle structure (crystals) by SEM. Any feedback from
} those with similar projects/experiences would be very welcome (on or off the
} list). Even better would be references to any published reports of this
} kind of material exam.
}
} Thanks,
} Brad Huggins
} BPAmoco,
} Electron Microscopy Lab
} hugginbj-at-bp.com
}
}
}



From daemon Wed Mar 15 07:30:04 2000



From: Debby Sherman :      sherman-at-btny.purdue.edu
Date: 15 Mar 2000 08:25:42 -0500
Subject: Re: LR-White polimerization

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From: Debby Sherman :      sherman-at-btny.purdue.edu
Date: 15 Mar 2000 08:25:42 -0500
Subject: Re: LR-White polimerization

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


You have fallen into the same trap I did some time back. Some suppliers ship the LR White resin with the catalyst already included. Others do not, sending the catalyst along with instructions to add it prior to using the resin. The reason to not mix the ingredients prior to shipping is that if the resin is subject to hot temperatures or prolonged shipping time, the resin mix could be compromised. This is a good point which is certanly valid when shipping resin in the summer or to remote locations.

However, most of us do not run into these problems and are used to receiving the resin in the complete mixed form. In my case,I ordered from a different supplier than normal. The labeling of the bottles was not clear and I did not realize that the enclosed "extra" ingredient was the catalyst, not the accelerator used for cold curing polymerization, until I also ran into the problem of the resin not polymerizing as expected.

Moral of the story....always read the fine print...both in the catalog and after receiving the product, especially when ordering from a different supplier than in the past.

Debby

Debby Sherman, Manager Phone: 765-494-6666
Microscopy Center in Agriculture FAX: 765-494-5896
Dept. of Botany & Plant Pathology E-mail: sherman-at-btny.purdue.edu
Purdue University
1057 Whistler Building
West Lafayette, IN 47907-1057

On Tuesday, March 14, 2000, A.P. Alves de Matos {apmatos-at-ip.pt} wrote:
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From daemon Wed Mar 15 08:14:17 2000



From: Buddy Steffens :      steffens-at-calc.vet.uga.edu
Date: Wed, 15 Mar 2000 09:10:26 -0500
Subject: Re: Immunoelectron microscopy of myelinated nerve

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I have recently performed an immunoEM labeling procedure with
} myelinated nerve embedded in Lowicryl, K4M resin. Unfortunately the
} myelin is severely altered (it appears as large clealr blebs) because I
} am not able to osmicate this tissue due to the UV polymerization of
} this resin. Does anyone have any suggestions as to preservation of
} lipid laden myelin without the use of osmium? I used methanol in the
} dehydration procedure which has probably caused this artefact....

Karen,

It sounds like the myelin is being partially extracted during dehydration
because of lack of OsO4 stabilization. You might try acrolein...it is the only
fixative other than OsO4 that will preserve and retain lipids. Make sure that
you read all the precautions about it...its pretty nasty stuff.




-=W.L. Steffens=-
Department of Veterinary Pathology
College of Veterinary Medicine
University of Georgia
Athens, GA 30602 USA
http://www.vet.uga.edu/vpp/wls/steffens.html



From daemon Wed Mar 15 08:34:34 2000



From: Leona Cohen-Gould :      lcgould-at-mail.med.cornell.edu
Date: Wed, 15 Mar 2000 10:47:23 -0400
Subject: Re: Toluidine blue stain

Contents Retrieved from Microscopy Listserver Archives
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At 1:18 PM -0700 3/14/0, Soumitra Ghoshroy wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

After your sections have been dried onto the slide, cover them with a drop
of stain for 30 sec - 1 min. and wash off with water. Dry.

If its too light, repeat.

Lee

Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Confocal Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175




From daemon Wed Mar 15 08:34:34 2000



From: William Tivol :      tivol-at-wadsworth.org
Date: Wed, 15 Mar 2000 09:22:08 -0500
Subject: Re: Cryo-microscopy of solid mineral oils

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Huggins, Bradley J wrote:

} We are trying to get a project started that involves imaging the
} microstructure of mineral oils and polyalphaolefins as they solidify. The
} solid temperature range for materials like these is approximately -20 to -70
} C. Does anyone have experience with these or similar materials. We are
} hoping to image the particle structure (crystals) by SEM. Any feedback from
} those with similar projects/experiences would be very welcome (on or off the
} list). Even better would be references to any published reports of this
} kind of material exam.
}
}

Dear Bradley,
Doug Dorset has done extensive work with electron crystallography
of waxes and lipids. He does electron diffraction with a TEM, but perhaps
his results will be useful to you.
Yours,
Bill Tivol



From daemon Wed Mar 15 09:04:12 2000



From: Anne Heller :      heller-at-Uni-Hohenheim.DE
Date: Wed, 15 Mar 2000 15:50:31 +0100
Subject: Re: Toluidine blue stain

Contents Retrieved from Microscopy Listserver Archives
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Dear Soumitra Ghoshroy,

for methacrylate sections we use 0.5 % Toluidinblue in 0.1 mol phosphate
buffer (it should also work just with water) and for epoxide sections 0.1%
Toluidinblue in 2% Na-bicarbonate. staining time depends on temperature,
thickness, and material. After staining wash with water and dry the sections.

regards,
Anne


Soumitra Ghoshroy schrieb:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Dear Microscopists,
}
} Does anyone have a protocol for making Toluidine blue stain to be used for
} staining thick sections of resin embedded biological samples ?
}
} Thanks a lot,
}
} Soumitra
}
} *****************************************************************
} Soumitra Ghoshroy Ph.D.
} Electron Microscopy Lab
} Box 3EML
} New Mexico State University
} Las Cruces, NM 88003
} Tel: 505-646-3600
} Fax: 505-646-5665
} e-mail: ghoshroy-at-nmsu.edu
} http://confocal.nmsu.edu/eml

--
Dr. Anne Heller, AG Elektronenmikroskopie,
Institut fŸr Botanik (210), UniversitŠt Hohenheim,
Garbenstra§e 30
D-70593 Stuttgart

Tel.: (0049)-711-459-2180
Fax.: (0049)-711-459-3355




From daemon Wed Mar 15 09:34:08 2000



From: Walck. Scott D. :      walck-at-ppg.com
Date: Wed, 15 Mar 2000 10:24:13 -0500
Subject: RE: TEM cross section preparation

Contents Retrieved from Microscopy Listserver Archives
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I forgot another cheaper alternative that I have used in the past. You can
use a small, medium, or large binder clip to clamp samples together
depending on their size. You can use Teflon tape on the surface to prevent
sticking to the clamp. If you make a little Teflon jig to hold the sample
it works quite well.
-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)


"The opinions expressed are those of Scott D. Walck and not of PPG
Industries, Inc. nor of any PPG-associated companies."
--



} -----Original Message-----
} From: Leroux christine [mailto:leroux-at-univ-tln.fr]
} Sent: Tuesday, March 14, 2000 9:45 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: TEM cross section preparation
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.} html
}
}
} --------------------------------------------------------------
} ---------.
}
}
} hello,
} I have to prepare cross sectionning samples, and I do not
} know where to
} buy the small
} vise that I need to clamp the specimen when I have glued
} them (I have
} already the
} Mbond 610 adhesive).
} I would greetly appreciate any information about this thing
} (I am not even
} sure of the
} name of it)
} thank you in advance
} christine
}
} **************************************
} Christine leroux
} Lab. M.M.I
} Universite de Toulon-Var, Bat.R
} B.P.132
} F-83957 La Garde Cedex
} tel: 00 33 (0) 4 94 14 25 07
} fax: 00 33 (0) 4 94 14 21 68
}
} **************************************
}
}



From daemon Wed Mar 15 10:24:03 2000



From: teresa_boes-at-hp.com
Date: Wed, 15 Mar 2000 08:17:38 -0800
Subject: Vibration isolation tables

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ann,
I'm also looking for vibration isolation tables for ultramicrotomy.
I've been looking at Vibraplane (http://www.kineticsystems.com) for isolation
tables and bench-top isolation platforms, which are also sold through stereo
equipment companies (i.e. http://www.soundsofsilence.com
http://www.audioodyssey.com). I need a height-adjustable isolation table -
other vibration sensitive equipment in our lab is successfully being used on the
isolation platforms on top of separate height-adjustable tables. I would
appreciate hearing of any information you receive offline about the isolation
tables.

Teresa Boes
Analytical Services Lab
Hewlett-Packard
Corvallis, OR
541-715-7055
teresa_boes-at-hp.com

-----Original Message-----
} From: Lehman, Ann [mailto:Ann.Lehman-at-trincoll.edu]
Sent: Tuesday, March 14, 2000 6:37 AM
To: 'MSA Listserver'


Dear Listers,

I'm looking for a source for vibration isolation tables for ultramicrotomy.
The marble tables that we are using now are not adequate (except during
Spring Break when no one is in the building...) I'm thinking of a system
that uses compressed gas (N2?) to float the tables.

Please respond offline (vendors welcome).

thanks, all

Ann Hein Lehman
EM Facility Mgr
Trinity College
Hartford CT
860-297-4289
860-297-2538
ann.lehman-at-trincoll.edu



From daemon Wed Mar 15 10:33:59 2000



From: sherwood-at-helix.mgh.harvard.edu (Peggy Sherwood)
Date: Wed, 15 Mar 2000 11:32:00 -0500
Subject: Re: Toluidine blue stain

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} Date: Wed, 15 Mar 2000 11:15:59 -0500
} To:Soumitra Ghoshroy {ghoshroy-at-nmsu.edu}
} From:sherwood-at-HELIX.MGH.HARVARD.EDU (Peggy Sherwood)
} Subject:Re: Toluidine blue stain
}
} } Soumitra,
}
} We use a 0.5% Toluidine Blue in Borate Buffer (1 gram Toluidine Blue, 1
} gram sodium borate and 200ml water). Tousimis (and maybe other suppliers)
} sells a Methylene Blue- Toludine Blue stain for epoxy stain (Cat. # 4166B)
} which we have used: (consists of 0.2% methylene blue & 1.0% toluidine
} blue certified stains in Na-Borate buffer). Either way--we always filter
} our stains (using a syringe with filter unit attached) right onto
} sections. Then we put slide ( super frost plus, so sections will adhere)
} on hot plate (temp. -at- 60 decrees C). We rinse sections with water and
} then check stain--time is arbitrary: depending upon tissue type & section
} thickness as to how dark or light your stain result.
}
} When I worked in Ophthalmology research, I used a wonderful dichromatic
} stain: methylene blue-azure II-basic fuchsin stain. It was similar to H
} & E but for epoxy sections. (made great 2X2 slides). The reference is:
} Humphrey, C.D. and Pittman, F.E. (1974) Stain Technology 42:9-14. I
} actually have a copy that came as an LKB application note 303 which I
} could fax to you. Feel free to contact me off line. Good luck!
}
} Peggy Sherwood
} Wellman Labs of Photomedicine
} Lab Associate-Photopathology Lab
} Massachusetts General Hospital
} 50 Blossom Street
} Boston, MA 02114
} 617-726-6983
} 617-724-4839 (voice mail)
} 617-726-3192 (fax)
} sherwood-at-helix.mgh.harvard.edu
}
}
}
}
}
} ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From daemon Wed Mar 15 11:43:49 2000



From: Soumitra Ghoshroy :      ghoshroy-at-nmsu.edu
Date: Wed, 15 Mar 2000 10:35:17 -0700
Subject: Toluidine blue stain

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thanks to all of you who responded to my question about the stain.

This is a great microscopy resource.

Best wishes.


Soumitra



*****************************************************************
Soumitra Ghoshroy Ph.D.
Electron Microscopy Lab
Box 3EML
New Mexico State University
Las Cruces, NM 88003
Tel: 505-646-3600
Fax: 505-646-5665
e-mail: ghoshroy-at-nmsu.edu
http://confocal.nmsu.edu/eml




From daemon Wed Mar 15 11:53:49 2000



From: Mary Gail Engle :      mgengle-at-pop.uky.edu
Date: Wed, 15 Mar 2000 12:43:55 -0500
Subject: toluidine blue

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Soumitra,
We use the following recipe for toluidine blue:


These stock solutions can be made in large volumes and stored.
.5 % toluidine blue in distilled water
.25 % sodium borate in distilled water

Mix equal parts of the above.
Stain dried resin sections on hot plate (low setting) until edge of stain
begins to evaporate (5-10 secs)
Rinse, blot excess water from bottom of slide and dry on hot plate.

Mary Gail Engle
Electron Microscopy & Imaging Facility
University of Kentucky




From daemon Wed Mar 15 11:53:51 2000



From: Huggins, Bradley J :      HUGGINBJ-at-BP.com
Date: Wed, 15 Mar 2000 11:53:12 -0600
Subject: RE: Cryo-microscopy of solid mineral oils

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Patrick,
Are you telling me that in your book, you have a reference to some work
where some oil based fuels were cooled to a solid, and them imaged in that
frozen state? If so, this is precisely the kind of information that I would
like to see. If not, do you have a reference? I do not have a copy of
your book, but I have seen it, some years ago. In any case, thanks for the
reminder on that resource.

And even more specifically, if anyone has knowledge of any similar work done
on mineral oil and like materials... it sure would save me some work on the
development side...

Thanks for the feedback,
Brad

} ----------
} From: Dr P. Echlin[SMTP:pe13-at-cus.cam.ac.uk]
} Sent: Wednesday, March 15, 2000 6:43 AM
} To: Huggins, Bradley J
} Cc: Microscopy listserver
} Subject: Re: Cryo-microscopy of solid mineral oils
}
} Dear Bradley:
}
} I know oil based fuels have been imaged at low temperature. Suggest you
} read (better still, Buy) my book "Low Temperature Microscopy and
} Analysis" Plenum Press New York 1992. I would modestly suggest it has a
} lot of information about how to prepare, observe and analyse specimens
} at low temperature. My own current interests revolve around low volyage,
} low temperature x-ray microabalysis of bio-organic materials. Let me
} know if I can be of any further help
}
} Patrick Echlin
} University of Cambridge UK
}
} pe13-at-cus.cam.ac.uk
}
}
} On Tue, 14 Mar 2000, Huggins, Bradley
} J wrote:
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Hello all,
} } We are trying to get a project started that involves imaging the
} } microstructure of mineral oils and polyalphaolefins as they solidify.
} The
} } solid temperature range for materials like these is approximately -20 to
} -70
} } C. Does anyone have experience with these or similar materials. We
} are
} } hoping to image the particle structure (crystals) by SEM. Any feedback
} from
} } those with similar projects/experiences would be very welcome (on or off
} the
} } list). Even better would be references to any published reports of this
} } kind of material exam.
} }
} } Thanks,
} } Brad Huggins
} } BPAmoco,
} } Electron Microscopy Lab
} } hugginbj-at-bp.com
} }
} }
} }
}



From daemon Wed Mar 15 12:03:47 2000



From: Jose Maria Manero :      manero-at-cmem.upc.es
Date: Wed, 15 Mar 2000 18:59:22 +0100
Subject: [TEM] Problem with magnetic samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear all,

I want to observe pure iron by means of TEM (Jeol 1200 EX II) in order
to study the dislocations substructure. But I have some problems, for
example, I can not focus the image due to the magnetism of the ferrite.

I am looking for suggestions to avoid this problem. What could I do?

Thank you in advance.

Dr. J. M. Manero
Universidad PolitŽcnica de Catalunya.
E.T.S.I. Industriales de Barcelona. Spain.
e-mail: manero-at-cmem.upc.es




From daemon Wed Mar 15 12:03:47 2000



From: Mary Gail Engle :      mgengle-at-pop.uky.edu
Date: Wed, 15 Mar 2000 12:54:46 -0500
Subject: service contracts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ben,
If you have other microscopes on campus you might consider forming a
consortium of all the users and hiring your own on-site service engineer
to take care of all the instruments. We've been doing this since 1981 and
it's worked very well. One man's salary is considerably less than service
contracts on many instruments. You also have the luxury of instant service.
Mary Gail Engle
Electron Microscopy & Imaging Facility
University of Kentucky



From daemon Wed Mar 15 12:23:47 2000



From: rgriffin-at-eng.uab.edu
Date: Wed, 15 Mar 2000 12:13:38 -0600
Subject: [TEM] Problem with magnetic samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


1) Start (before final thinning) with the thinnest sample you can support
( {50 microns). Pure iron will be quite soft so you'll have to be very
careful not to bend it - especially since you're looking at dislocation
structure. You could support it on a copper key-grid to minimize damage. If
you have to electropolish while it is on the copper key-grid, you'll need to
lacquer the copper prior to electropolishing and then clean it off before
putting it into the microscope.
2) If you still can't focus, try taking it out of the microscope and
rotating it in the holder. Sometimes a different orientation really helps.
3 Plan on doing alignment every time you move or tilt. It is a given with
magnetic samples. You'll need to adjust the beam tilt and the astigmatism
continually. I always used the "dark-field" beam tilts as they are stronger
than the bright field set. You'll be amazed at how good you get at aligning
after a few months of magnetic work. Later work with non-magnetic samples
will be a breeze.
4) One final warning. The objective lens can actually suck your sample out
of the holder during insertion of the sample. If you have a really thin
sample, it can break off chunks. These chunks/sample will ALWAYS stick to
the lens and make every other user miserable. Your service guy will be
forced at some point to clean off the lens of your sample (or sample bits)
and you will be reviled. A simple solution is to always turn off the
objective lens when you are inserting and removing your sample.

Good luck - magnetic work is possible and fun once you get the hang of it.

Robin Griffin

-----Original Message-----
} From: Jose Maria Manero [mailto:manero-at-cmem.upc.es]
Sent: Wednesday, March 15, 2000 11:59 AM
To: Microscopy-at-sparc5.microscopy.com


Dear all,

I want to observe pure iron by means of TEM (Jeol 1200 EX II) in order
to study the dislocations substructure. But I have some problems, for
example, I can not focus the image due to the magnetism of the ferrite.

I am looking for suggestions to avoid this problem. What could I do?

Thank you in advance.

Dr. J. M. Manero
Universidad PolitŽcnica de Catalunya.
E.T.S.I. Industriales de Barcelona. Spain.
e-mail: manero-at-cmem.upc.es



From daemon Wed Mar 15 13:05:38 2000



From: O'Neil, David :      David.O'Neil-at-nrc.ca
Date: Wed, 15 Mar 2000 14:56:20 -0400
Subject: LM: Fluorescence lamp instability

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We've been having stability problems with our mercury vapor lamps on
our Reichert Polyvar microscope. We have been told by one source that the
power supply is likely the problem and another source tells us that the
bulbs are the problem. The source illumination, when imaged, can be seen to
flicker at random intervals and causes the illumination to vary. Does
anyone have experience with this microscope, and if so which brand of lamps
do you use? This microscope uses a 220W/4 L1 lamp. Thanks.


David O'Neil
National Research Council of Canada
Institute for Marine Biosciences
1411 Oxford Street
Halifax, NS B3H 3Z1
ph: (902)426-8258
fax: (902)426-9413
e-mail: david.o'neil-at-nrc.ca



From daemon Wed Mar 15 13:13:36 2000



From: Hendrik O. Colijn :      colijn.1-at-osu.edu
Date: Wed, 15 Mar 2000 14:07:40 -0500
Subject: RE: [TEM] Problem with magnetic samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Robin,

I certainly agree that EM of magnetic samples is quite doable, but I'm not
sure I'd agree that it is "fun".

Perhaps the best tip I can give is to reduce the amount of magnetic
material within the polepiece. I have cut my magnetic samples down to less
than the standard 3mm size and glued them securely onto a Cu slot
grid. The magnetic effects are much less of a problem.

Cheers,
Henk


At 12:13 PM 3/15/00 -0600, rgriffin-at-eng.uab.edu"-at-sparc5.microscopy.com wrote:
} {snip}

} Good luck - magnetic work is possible and fun once you get the hang of it.
}
} Robin Griffin

Hendrik O. Colijn colijn.1-at-osu.edu
Campus Electron Optics Facility Ohio State University
(614) 292-0674 http://web.ceof.ohio-state.edu
Murphy's Law: "If anything can go wrong, it will."
Commentary: "Murphy was an optimist."



From daemon Wed Mar 15 13:43:33 2000



From: George Lawton :      George.Lawton-at-email.swmed.edu
Date: Wed, 15 Mar 2000 13:34:49 -0600
Subject: Toluidine blue

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Soumitra,
We use a Toluidine Blue and Methylene blue staining solution:

Dissolve 0.5gm Borax in 95 ml DH2O, add 0.5gm of methylene blue, mix then add 5 ml of a 2% Toluidine blue solution in H2O.

Filter the stain just before staining. Stain for 1 minute on a hot plate set slightly lower than where the stain starts to bubble.
Rinse with DH2O and dry.

We like this stain because it is fast, very clean, and very pretty. We prefer this stain to
other Toluidine Blue solutions.

George

George Lawton
Chief Electron Microscopist
Molecular and Cellular Imaging Facility
UT Southwestern Medical Center at Dallas
5323 Harry Hines Blvd.
Dallas, Tx 75390-9039
Phone: 214-648-7291
eMail: George.Lawton-at-email.swmed.edu



From daemon Wed Mar 15 14:13:30 2000



From: Ron L'Herault :      lherault-at-bu.edu
Date: Wed, 15 Mar 2000 13:56:47 -0500
Subject: OT Spectrophotometer- B & L

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


A lab on our floor is having trouble with their B & L spectrophotometer.
does anyone know who, hopefully in New England, might be able to service
the unit?

Thanks.

Ron
mailto:lherault-at-bu.edu



From daemon Wed Mar 15 14:43:27 2000



From: SEMTRADER-at-aol.com
Date: Wed, 15 Mar 2000 15:41:23 EST
Subject: Wanted TN 2000

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greetings Listers,

Our old TN2000 (Tracor) nimbin pulse processor has bit the dust. If anyone
has one available for free, please let me know.

Thank you
Keith Brenna
609-658-3908



From daemon Wed Mar 15 16:53:05 2000



From: Ni, Chao-Ying :      CYNi-at-rodel.com
Date: Wed, 15 Mar 2000 17:48:02 -0500
Subject: [TEM] Problem with magnetic samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


1. Reduce the sample size as much as possible, glue a tiny Fe piece on a Cu
or other nonmagnetic grids, for example.
2. Turn off the objective lens as you load your sample.
3. Do beam alignment as many times as necessary.
-cy
Rodel Inc.
451 Bellevue Road
Newark, Delaware 19713
USA


-----Original Message-----
} From: Jose Maria Manero [mailto:manero-at-cmem.upc.es]
Sent: Wednesday, March 15, 2000 12:59 PM
To: Microscopy-at-sparc5.microscopy.com


Dear all,

I want to observe pure iron by means of TEM (Jeol 1200 EX II) in order
to study the dislocations substructure. But I have some problems, for
example, I can not focus the image due to the magnetism of the ferrite.

I am looking for suggestions to avoid this problem. What could I do?

Thank you in advance.

Dr. J. M. Manero
Universidad PolitŽcnica de Catalunya.
E.T.S.I. Industriales de Barcelona. Spain.
e-mail: manero-at-cmem.upc.es




From daemon Thu Mar 16 08:06:24 2000



From: Diana van Driel :      dianavd-at-eye.usyd.edu.au
Date: Thu, 16 Mar 2000 12:27:55 +1100
Subject: Vibration isolation tables

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Ann,

A simple construction we used for years with a heavy microscope was a
wooden frame standing on a tennis balls. The microscope was on the
2nd floor of a building by a busy road. Never a shake was recorded on
film!

Diana



Diana van Driel
Department of Clinical Ophthalmology
Sydney University
GPO Box 4337
Sydney NSW
AUSTRALIA 2001

Phone 61 2 938 27278/27395
Mob 0412 165 075
Fax 61 2 938 27318



From daemon Thu Mar 16 08:06:30 2000



From: Earl Weltmer :      earlw-at-pacbell.net
Date: Wed, 15 Mar 2000 19:12:46 -0800
Subject: Re: Amray 1910FE SEM maintenance contract

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


As an independent service provider of several SEM manufacturers for
over 20 years,
I think I can also comment & agree with Ken on several fronts concerning
Amray.
However, I don't want to get into a "bashing" of any given manufacturer,
at least online.
I will say that every Company (SEM or otherwise) has their own way of
doing things.
Each Company is different, depending upon the people running the
operation.

Many manufacturer's think they have a "lock" on the customer and on the
service because of their FE guns or perhaps on another part.
This reminds me of ETEC just before they got out of the SEM service
business.
ETEC had an epoxy encapsulated high voltage power supply that only ETEC
manufactured.
I inquired with ETEC about the cost of one of these units. ETEC
responded with a price tag of $50,000.00.
Yes $50,000.00 in 1982!
ETEC service also let it be known that they were the only source for
this part.
Furthermore, customers considering a third party for service were
informed (and threatened) by ETEC about this situation.
Customers were stuck paying ETEC's exorbitant maintenance costs.

The ETEC customers were hesitant to go to a third party service until
the high voltage issue had been resolved.
I spent many days (and nights) re-engineering another high voltage power
supply until I had it perfectly duplicated.
When customers would ask about the high voltage issue, I would show them
my version & invite them to use it in their system.
Within two years I had over 50% of ETEC's service business at 60% of
ETEC contract costs.

Today several manufacturers still think they have a "lock" on service.
I have been informed by one manufacturer that this is their way of
"putting you (third party maintenance) out of business".
If I had the time and the inclination, I would spend it duplicating the
FE gun some of these manufacturer's think they have a "lock" on.
I have considered such a move because no one has a "lock" on technology.

I have had several requests but have not responded because the time &
effort necessary is not viable for only three customer requests..
Fortunately for the manufacturer, I am busy and, quite frankly, not as
"hungry" as I used to be. Maybe it comes with age.
If I ever get "unhungry" or not as busy, I would take these
manufacturer's up on their smugness and beat them at their own game by
duplicating their FE guns.
The ultimate winner is the customer.

The only manufacturer that has a great gun that can be serviced by the
customer is Hitachi.
Hitachi has the patent on an internal heater for their gun which makes
exchanging FE guns obsolete.
Hitachi FE guns can be re-conditioned by the customer in-house at a cost
of $750.00 instead of $7,000.00 for Amray or $17,000.00 for JEOL.
Hitachi is doing well and doesn't need to worry about "locking" third
party maintenance or anyone else out.
They have too many sales to worry about & don't have the time to be
petty.

Great thing about the free enterprise system is that real talent always
has customers "beating a path" to their door.
Marginal talent needs to cajole customers to their way of thinking.
Great talent has customers calling on them.


Regards,

Earl Weltmer
Scanservice Corporation
Third Party Maintenance


At 01:13 PM 3/14/00 , you wrote:


} -----------------------------------------------------------------------.

[snip]

} Gary,
} Your earlier comments on how good AMRAY is really puzzle me, but be
that
} as it may, if any of their Federal Government customers are on the
ball,
} Amray will be told, as was Perkin Elmer ETEC, that they must support
the
} equipment for about 7 years or face lawsuits. There is an implied
} responsibility when one sells expensive equipment.
}
} Perhaps supplying parts and detailed instructions will cover them
} legally, in which case we third party folks may be able to help out.
If
} anyone has any more detailed info on Amray service, I would love to
know
} because I have serviced them off and on over the years and would be
} willing to help out here in the Northeast/Mid-Atlantic area.
}
} Ken Converse
} owner
} Quality Images
} third party SEM service
} 16 Creek Rd.
} Delta, PA 17314
}
} 717-456-5491

I take it that you don't think that Amrays are very good. What aspect

of them do you find lacking? I would tend to agree that the early
models
are no comparison to their generations of the last 6-10 years. The FE

systems are particularly good. My 1910FE does a great job being an
FE and having the flat lens. The 1800 series with conical lenses are
not as good, but are required when doing IC wafers.

You have a great point about US Gov users. I know several of them.
When does the 7 years start? From what event or timepoint?
Amray has not stopped providing service. But the word is that they
most likely will in about 2 years....based on their takeover by
KLA-Tencor.

One fellow pointed out the proprietary nature of the FE gun. Without
access to this, third party providers cannot work on the systems....at

least not on the gun assembly.

tnx,
gary



From daemon Thu Mar 16 08:06:33 2000



From: Joss, Michael :      Michael.Joss-at-dpr.csiro.au
Date: Thu, 16 Mar 2000 14:45:25 +1100
Subject: FM:Non-fluorescent epoxy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We are trying to examine oil inclusions trapped in quartz under UV light.
The oil fluoresces and the quartz does not. Sounds easy. Unfortunately the
epoxy that the sample is embedded in also fluoresces quite strongly making
identification difficult. So I was wondering if there are any
non-fluorescent epoxies available on the market, or alternatively if there
are any pigments/dyes that can be added to the epoxy to reduce its
fluorescence. If not then perhaps an alternative to embedding in epoxy might
be the best option....

Any suggestions warmly welcomed.
:-)

Michael Joss
Quantitative Microscopist
CSIRO Division of Petroleum Resources
Fluid History Analysis Group
Phone: 9490 8148
Fax: 9490 8467



From daemon Thu Mar 16 08:06:37 2000



From: Joss, Michael :      Michael.Joss-at-dpr.csiro.au
Date: Thu, 16 Mar 2000 15:21:18 +1100
Subject: FM:Non-fluorescent epoxy

Contents Retrieved from Microscopy Listserver Archives
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} We are trying to examine oil inclusions trapped in quartz under UV light.
} The oil fluoresces and the quartz does not. Sounds easy. Unfortunately the
} epoxy that the sample is embedded in also fluoresces quite strongly making
} identification difficult. So I was wondering if there are any
} non-fluorescent epoxies available on the market, or alternatively if there
} are any pigments/dyes that can be added to the epoxy to reduce its
} fluorescence. If not then perhaps an alternative to embedding in epoxy
} might be the best option....
}
} Any suggestions warmly welcomed.
} :-)
}
} Michael Joss
} Quantitative Microscopist
} CSIRO Division of Petroleum Resources
} Fluid History Analysis Group
} Phone: 9490 8148
} Fax: 9490 8467
}



From daemon Thu Mar 16 08:06:37 2000



From: Joss, Michael :      Michael.Joss-at-dpr.csiro.au
Date: Thu, 16 Mar 2000 15:23:36 +1100
Subject: Non-fluorescent epoxy

Contents Retrieved from Microscopy Listserver Archives
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} We are trying to examine oil inclusions trapped in quartz under UV light.
} The oil fluoresces and the quartz does not. Sounds easy. Unfortunately the
} epoxy that the sample is embedded in also fluoresces quite strongly making
} identification difficult. So I was wondering if there are any
} non-fluorescent epoxies available on the market, or alternatively if there
} are any pigments/dyes that can be added to the epoxy to reduce its
} fluorescence. If not then perhaps an alternative to embedding in epoxy
} might be the best option....
}
} Any suggestions warmly welcomed.
} :-)
}
} Michael Joss
} Quantitative Microscopist
} CSIRO Division of Petroleum Resources
} Fluid History Analysis Group
} Phone: 9490 8148
} Fax: 9490 8467
}



From daemon Thu Mar 16 08:06:37 2000



From: Vitaly Feingold :      vitalylazar-at-worldnet.att.net
Date: Wednesday, March 15, 2000 10:25 PM
Subject: Vibration isolation tables

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Dear Diana,

The tennis balls will isolate vibration nearly perfect. We used this
approach a lot in Russia simply because we had no other choice. But tennis
balls have tendency to deflate under pressure of the weight of the machine.
The deflation will become noticeable in a few moths. Then the entire frame
has to be lifted in order to replace tennis balls. Much better approach is
to use pneumatic antivibration mounts by Barry Controls. They come in
different sizes for loads from 100 lb. to several tons, and available from a
number of suppliers including McMaster-Carr.
Unless, of course, Australian tennis balls are superior to Russian ones...
Cheers.

Vitaly Feingold
Scientific Instruments and Applications
2773 Heath Lane, Duluth GA 30096
(770)232-7785 ph.
(770)232-1791 fax
-----Original Message-----
} From: Diana van Driel {dianavd-at-eye.usyd.edu.au}
To: MicroscopyList {microscopy-at-sparc5.microscopy.com}



From daemon Thu Mar 16 08:06:38 2000



From: EM Unit (HISTO) :      emunit-at-mail.wch.sa.gov.au
Date: Thu, 16 Mar 2000 15:29:00 +1030
Subject: Scanning EM.

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We are interested in obtaining a second hand scanning EM. within Australia
at minimal cost. Please email us personally.

Thanks in advance,

Ruth and Alvis



From daemon Thu Mar 16 08:06:40 2000



From: Ron Doole :      ron.doole-at-materials.oxford.ac.uk
Date: Thu, 16 Mar 2000 08:26:58 +0000 (GMT Standard Time)
Subject: Re: [TEM] Problem with magnetic samples

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Hi,

I have read the previous suggestions and would
agree with them - the message is get as much of that magnet
out of your microscope as possible. However other tips that
you may find useful:

If you have a side entry stage you will find it
very difficult to set the eucentric height with a magnetic
specimen. Either note the objective lens current at
eucentric focus and set that or focus a non magnetic
specimen at eucentric and then insert your secimen. Having
done that focus the specimen using the eucentric height
control.

Try to have as small a hole as possible in your specimen.
This will reduce the effect of having a magnet one side of
the beam and not the other. Of course the best specimen
would not have a hole but just uniform thin area.

You will have to realign the beam every time you move the
specimen.

When out of focus you may see the Fresnel image of Domain
walls, when they change contrast from black to white (or
vice versa) then you are in focus.

Good luck,
Ron


On Wed, 15 Mar 2000 18:59:22 +0100 Jose Maria Manero
{manero-at-cmem.upc.es} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear all,
}
} I want to observe pure iron by means of TEM (Jeol 1200 EX II) in order
} to study the dislocations substructure. But I have some problems, for
} example, I can not focus the image due to the magnetism of the ferrite.
}
} I am looking for suggestions to avoid this problem. What could I do?
}
} Thank you in advance.
}
} Dr. J. M. Manero
} Universidad Politécnica de Catalunya.
} E.T.S.I. Industriales de Barcelona. Spain.
} e-mail: manero-at-cmem.upc.es
}
}
}

----------------------
Mr. R.C. Doole
Department of Materials,
University of Oxford.
Parks Road, Oxford. OX1 3PH. UK.
Phone +44 (0) 1865 273701
Fax +44 (0) 1865 283333
ron.doole-at-materials.ox.ac.uk



From daemon Thu Mar 16 08:06:44 2000



From: jim :      jim-at-proscitech.com.au
Date: Thu, 16 Mar 2000 21:47:41 +1000
Subject: RE: Toluidine blue stain

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I've read most of the replies but found no mention of differentiation. This is
one of the most attractive features of the toluidine stain as it can give a two
colour, pink and blue rendition. This shows cell features similar to a double
staining with Haematoxylin & Eosin.

After staining on a hotplate add a drop of acid alcohol and then rinse the
slide with distilled water. The acid alcohol can destain partially or even
completely, but a brief application brings forth the two colours.

Make acid alcohol by adding to 25ml of 50% Ethanol a couple of drops of 1N HCl.
Cheers
Jim Darley

ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Wednesday, March 15, 2000 6:18 AM, Soumitra Ghoshroy
[SMTP:ghoshroy-at-nmsu.edu] wrote:
}
} Dear Microscopists,
}
} Does anyone have a protocol for making Toluidine blue stain to be used for
} staining thick sections of resin embedded biological samples ?
}
} Thanks a lot,
}
} Soumitra
}
} *****************************************************************
} Soumitra Ghoshroy Ph.D.
} Electron Microscopy Lab
} Box 3EML
} New Mexico State University
} Las Cruces, NM 88003
} Tel: 505-646-3600
} Fax: 505-646-5665
} e-mail: ghoshroy-at-nmsu.edu
} http://confocal.nmsu.edu/eml



From daemon Thu Mar 16 08:06:45 2000



From: Heisel, Christian, Dr. :      Christian.Heisel-at-ok.uni-heidelberg.de
Date: Thu, 16 Mar 2000 12:52:08 +0100
Subject: Subject: SEM - Problems with marking bacteria

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We are examining Staphylococcus epidermidis on the surface of acrylic bone
cement. We have problems with the marking of bacteria. Artefacts of
particles from the Polymethylmethacrylate (bone cement) look nearly the same
than the bacteria. We fix with glut, dehydrate, do critical point drying and
coat with gold afterwards. Does anyone have experience with marking bacteria
in SEM to surely identify them?
Thank you in advance,
Christian Heisel, M.D.
University of Heidelberg
Dep.of Orthopedics
Schlierbacher Landstra§e 200 A
69118 Heidelberg
Germany
Phone:0049-6221-965-
Fax:0049-6221-966121
e-mail:christian.heisel-at-ok.uni-heidelberg.de



From daemon Thu Mar 16 08:06:48 2000



From: Alexander Mironov :      amironov-at-nki.nl
Date: Thu, 16 Mar 2000 13:38:40 +0100
Subject: Re: Immunoelectron microscopy of myelinated nerve

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} I have recently performed an immunoEM labeling procedure with
} } myelinated nerve embedded in Lowicryl, K4M resin. Unfortunately the
} } myelin is severely altered (it appears as large clealr blebs) because I
} } am not able to osmicate this tissue due to the UV polymerization of
} } this resin. Does anyone have any suggestions as to preservation of
} } lipid laden myelin without the use of osmium? I used methanol in the
} } dehydration procedure which has probably caused this artefact....

Karen,

Try to switch to cryosections. This reference could help in solving your problem.
Liou W, Geuze HJ, Slot JW Histochem Cell Biol 1996 Jul;106(1):41-58
--
Alexander Mironov Jr.
Division of Tumor Biology, H4
The Netherlands Cancer Institute
Plesmanlaan 121
1066 CX Amsterdam

Tel. +31-020-512-2017
Fax +31-020-512-2029
E-mail: amironov-at-nki.nl




From daemon Thu Mar 16 08:06:50 2000



From: Leslie Eibest :      leibest-at-duke.edu
Date: Thu, 16 Mar 2000 08:22:42 -0500
Subject: Multi-user facility managers

Contents Retrieved from Microscopy Listserver Archives
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Hi, folks;

One concern raised by a granting agency about our proposal
for new equipment for a multi-user SEM lab was our lack of a
management plan (primarily for time allocation among users and for
conflict resolution). We have been operating under a first
come-first served policy, and conflicts have not been a problem.
I would greatly appreciate insights from managers of
multi-user facilities of any sort as to your approaches to allocation
of access to instrumentation, and methods of resolving conflicts
among researchers.

Thanks for any information!

Leslie Eibest
Zoology Dept.
Duke University



From daemon Thu Mar 16 08:06:50 2000



From: LAB. DE MICROSCOPIA ELECTRONICA - FI - UNER :      microsc-at-fi.uner.edu.ar
Date: Thu, 16 Mar 2000 10:26:09 +0000
Subject: looking for parts to TEM Philips EM 201

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Hello, microscopist's.......
I 'm looking for any provider of the parts listed above for an old Philips TEM
EM 201

PW6543 Plate numbering device
PW 6542 automatic exposure kit

any help is welcome......

thanks.....

fernando
===================================================
Fernando D. Balducci
Laboratorio de Microscopia Electr—nica
Facultad de Ingenier’a - Bioingenieria
Universidad Nacional de Entre Rios
Argentina
e-mail: microsc-at-fi.uner.edu.ar
tel: 54 43 975100
fax: 54 43 975077


===================================================



From daemon Thu Mar 16 08:06:51 2000



From: Lab. de Microscop’a :      microsc-at-fi.uner.edu.ar
Date: Thu, 16 Mar 2000 10:46:09 +0000
Subject: looking for parts to TEM Philips EM 201

Contents Retrieved from Microscopy Listserver Archives
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Hello, microscopist's.......
I 'm looking for any provider of the parts listed above for an old
Philips TEM
EM 201

PW6543 Plate numbering device
PW 6542 automatic exposure kit

any help is welcome......

thanks.....

fernando




From daemon Thu Mar 16 08:06:52 2000



From: Robin Schaublin :      Robin.Schaeublin-at-psi.ch
Date: Thu, 16 Mar 2000 14:54:56 +0000
Subject: Re: [TEM] Problem with magnetic samples

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The technique we now use on Fe and ferritic steels allows getting
an excellent behaviour in the TEM:

-A 1 mm hole is punched in the centre of a 3 mm TEM disk made out
of stainless steel (e.g. SS 316) about 300 micron thick,
-A 1 mm disk is punched from our ferritic specimen (about
300 micron thick),
-This 1mm disk is inserted - slightly forced - in the hole of
the 3 mm disk,
-A drop of glue (epoxy) is applied (to make sure there is no leak
during the electropolish that follows).
The 3 mm disk holding the 1 mm disk can then be used as a
normal TEM disk:
-Mechanical polish down to about 50-80 microns,
-Electropolishing with a 10 % vol. perchloric/methanol solution, 18V,
0C.

The final specimen is strong and the remaining magnetism is
minimal in the TEM: we can now move and tilt around without too much
realignment. Note that a puncher of high quality is essential. The one
from 'Eckert' doesn't introduce visible deformation in the centre
of the specimen; we tested that in annealed pure Fe.

-- -
Robin Schaeublin
CRPP - EPFL
5232 Villigen PSI
SWITZERLAND
Tel + 41 56 310 40 82
Fax + 41 56 310 45 29
Robin.Schaeublin-at-psi.ch
http://psgi05.psi.ch/
http://www.ssom.ch/




From daemon Thu Mar 16 17:42:15 2000



From: Purdy, Sam :      SPurdy-at-nationalsteel.com
Date: Thu, 16 Mar 2000 08:17:39 -0600
Subject: RE: Fluorescence lamp instability

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Dear David:
We have 15 year old Reichert Polyvar which, so far, has given us
little trouble. We use Osram HLX Xenophot bulbs, 12V, 100 W. Oddly enough,
we also have a Reichert MeF3 metallograph, about 10 years old.The light on
this instrument flickers erraticly. We replaced the lamp control board with
no success, switched lamps, etc. To no avail.Our service man thinks the
trouble lies in the transformer, which is no longer available. We are buying
a new separate light source but have not received it yet. We are not happy
with Reichert/Leica concerning assistancein this problem. They have been
less than helpful. On the other hand the service man(Dave Olney) from W.
Nuhsbaum, McHenry IL, has been very helpful.
I would be interesrted in whatever solution to this problem that you
may come up with. How old is your instument?

I have financial interest in either Reichert/Leica or W. Nuhsbaum.
} ----------
} From: O'Neil, David
} Sent: March 2000 1:56 PM
} To: Microscopy Listserver
} Subject: LM: Fluorescence lamp instability
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
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} -----------------------------------------------------------------------.
}
}
} We've been having stability problems with our mercury vapor lamps on
} our Reichert Polyvar microscope. We have been told by one source that the
} power supply is likely the problem and another source tells us that the
} bulbs are the problem. The source illumination, when imaged, can be seen
} to
} flicker at random intervals and causes the illumination to vary. Does
} anyone have experience with this microscope, and if so which brand of
} lamps
} do you use? This microscope uses a 220W/4 L1 lamp. Thanks.
}
}
} David O'Neil
} National Research Council of Canada
} Institute for Marine Biosciences
} 1411 Oxford Street
} Halifax, NS B3H 3Z1
} ph: (902)426-8258
} fax: (902)426-9413
} e-mail: david.o'neil-at-nrc.ca
}




From daemon Thu Mar 16 17:42:16 2000



From: Frank Thomas :      thomasf-at-AGC.BIO.NS.CA
Date: Thu, 16 Mar 2000 10:54:47 -0400
Subject: Re: Multi-user facility managers

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}
} Hi, folks;
}
} One concern raised by a granting agency about our proposal
} for new equipment for a multi-user SEM lab was our lack of a
} management plan (primarily for time allocation among users and for
} conflict resolution). We have been operating under a first
} come-first served policy, and conflicts have not been a problem.
} I would greatly appreciate insights from managers of
} multi-user facilities of any sort as to your approaches to allocation
} of access to instrumentation, and methods of resolving conflicts
} among researchers.
}
} Thanks for any information!
}
} Leslie Eibest
} Zoology Dept.
} Duke University
} Leslie -

My method, FWIW, is simplicity itself. I have a scheduling calendar posted
on the outer door of the lab, with a pen provided. Clients simply sign
themselves up in whatever day/time slot they wish, provided it's still
open. They can even sign up for days/times in future months, if they so
desire. Haven't caught anyone erasing a previous booking yet....but then
that's why I use a pen.....
This method certainly favours the folks who have their act together far
enough in advance so that they can schedule the instruments at their most
convenient times. Clients who show up at the last minute looking for
openings at their prefered time may well be out of luck. That's what we
call a learning experience.
I'm sure there is software available that can be mounted on your local
network which would do exactly the same thing, but, you know, I think it
makes more of a commitment for the client to actually make a trip down to
the lab to physically sign up, and then they're less likely to forget about
the scheduled session later.
It's worked pretty well for me for about 5 years now, and haven't seen a
good screaming match yet.


Frank Thomas
MicroAnalysis Facility
Geological Survey of Canada (Atlantic)
Bedford Institute of Oceanography
Dartmouth, Nova Scotia



From daemon Thu Mar 16 17:42:26 2000



From: Leona Cohen-Gould :      lcgould-at-mail.med.cornell.edu
Date: Thu, 16 Mar 2000 12:36:41 -0400
Subject: Re: Multi-user facility managers

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} Hi, folks;
}
} One concern raised by a granting agency about our proposal
} for new equipment for a multi-user SEM lab was our lack of a
} management plan (primarily for time allocation among users and for
} conflict resolution). We have been operating under a first
} come-first served policy, and conflicts have not been a problem.
} I would greatly appreciate insights from managers of
} multi-user facilities of any sort as to your approaches to allocation
} of access to instrumentation, and methods of resolving conflicts
} among researchers.
}
} Thanks for any information!
}
} Leslie Eibest
} Zoology Dept.
} Duke University

*****************************
Dear Leslie,
I have run a multi-user EM facility for 12 years, a now also manage an
optical microscopy facility. Both are core facilities for the medical
school.
For both facilities, users must be trained in the operation of the
instruments before they are given free access (we have coded locks).
During training, people set up appointments with me. Once "cleared fro
soloing" they are given an idivualized code and are then free to reserve
the instruments by means of a weekly sign-up sheet. For now, the sheet for
the following week is posted on Thursday or Friday. Our in-house computing
people keep promising us an on-line sign up. I can't wait since the 2
facil/lab group may reserve on a given day.
This has worked well for us so far. It also seems to satisfy the granting
instituions.
For more details, you can check out the school's web site
(http://www.med.cornell.edu/research/cores/index.html)select either
electron microscopy or optical microscopy to get the operating procedures.

Lee

Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Confocal Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175




From daemon Thu Mar 16 17:42:26 2000



From: Andrew Coutermarsh :      acoutermarsh-at-omegafilters.com
Date: Thu, 16 Mar 2000 11:21:55 -0500
Subject: JOB POSTING

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FOR ALL INTERESTED PARTIES:

Company: Omega Optical, Inc.

Location: Brattleboro, Vermont

Company
Description: 30-year old designer and manufacturer of thin film optical
interference filters used world-wide for laboratory and scientific
equipment.

Position: Fluorescence Product Manager

Position
Description: The Fluorescence Product Manager is responsible for any and
all activities which help further the companyâs sales of fluorescence
products. These activities include:

1. Technical sales and sales support: Technical sales and assistance to
sales force regarding fluorescence applications.
2. Marketing: Marketing activities related to fluorescence products.
3. Product & Market Development: Development activities related to new
fluorescence products, applications, and developing markets.

Omega is seeking an individual who is excited about working with varied
applications of fluorescence detection in the life sciences. The work
involves discussing the best match between laboratory experiments and
Omegaâs wide range of light filters for this science. Experience in
fluorescence, microscopy, life sciences and instrumentation is
essential.

Salary: Competitive with full benefit package.

Contact
Information: Email response with attached Word file resume and salary
history to: acoutermarsh-at-omegafilters.com or snailmail response to:


Andrew Coutermarsh, SPHR, HR Director
Omega Optical, Inc.
PO Box 573
Brattleboro, VT 05302
==============================================================




From daemon Thu Mar 16 17:42:27 2000



From: John Shields :      jpshield-at-arches.uga.edu
Date: Thu, 16 Mar 2000 11:34:59 -0500 (Eastern Standard Time)
Subject: references on optimal animal tissue fix

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Hello everyone,
We are having to justify using cervical dislocation without
anesthesia on a mouse prior to removing the liver for fixation for
our EM class.
Does anyone have a reference on hand that we can cite to show that
direct cervical dislocation is better (or not) for best preservation
of tissue under the circumstances? We won't be doing perfusion,
which would obviously provide better fix.
Please respond directly to me at: jpshield-at-arches.uga.edu

Background:
The class is held once a year, and one mouse is used (usually an
extra, "too old" mouse from the monoclonal facility).
The animal-use officer wants us to hike down to another building with
the class and use CO2 anesthesia, prior to euthanasia, so the mouse
suffers less (?). Then hike back up the hill with the tissue in
fixative. We've never had a problem with our animal-use request until
this year and the guy is busting our chops.

Thanks in advance,
john
********************************************
John P. Shields
Center for Ultrastructural Research
151 Barrow Hall
University of Georgia
Athens, GA 30602-2403
(706)542-4080
jpshield-at-arches.uga.edu




From daemon Thu Mar 16 17:42:28 2000



From: Elaine Humphrey :      ech-at-unixg.ubc.ca
Date: Thu, 16 Mar 2000 08:45:23 -0800
Subject: Re: Multi-user facility managers

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Hi Leslie
We are a multi-user facility, used by the Faculty of Medicine and the
Faculty of Science. Last fiscal year we had 653 individual users, the
majority frequent users. We have TEM, SEM, confocal,
fluorescent/brightfield, darkrooms and digital imaging equipment.

We use the first-come, first-served approach for many years and so far
there hasn't been a problem with sign-up conflicts with the instruments.
Most people around here are reasonable, thinking people.

We do not give open access after hours (9-5). But if a user is experienced
with the instrument, and I know he/she is not going to mess it up for the
next user, and he/she will take a responsibility lecture from me, there is
a floating key. I used to be more lax with letting users in after hours,
but after a week of just trouble shooting, I got tough. Since then, it has
worked well. I remember when I did my PhD, how much more work I got done
when there were no interuptions or distractions. So I have a sympathy for
allowing responsible researchers in when I am not there, especially as this
is a busy facility during the week day.

If it ever came to a conflict of access to the instruments, I am in charge.
I would think poorly of researchers who didn't play by the rules. The only
time, any problem has occured has been when someone has a crutial deadline.
Then when the situation has been put to the person who has it booked, they
can usually see themselves in that situation and have always been
accommodating. Maybe it is the Canadian way, but I should think it is far
more universal!

Good luck with your grant. Let me know if you need more information.
Elaine

}
} Hi, folks;
}
} One concern raised by a granting agency about our proposal
} for new equipment for a multi-user SEM lab was our lack of a
} management plan (primarily for time allocation among users and for
} conflict resolution). We have been operating under a first
} come-first served policy, and conflicts have not been a problem.
} I would greatly appreciate insights from managers of
} multi-user facilities of any sort as to your approaches to allocation
} of access to instrumentation, and methods of resolving conflicts
} among researchers.
}
} Thanks for any information!
}
} Leslie Eibest
} Zoology Dept.
} Duke University


Dr. Elaine Humphrey
Biosciences Electron Microscopy Facility
University of British Columbia
6270 University Blvd, mail-stop Botany
Vancouver, BC
CANADA, V6T 1Z4
Phone: 604-822-3354
FAX: 604-822-6089
e-mail: ech-at-unixg.ubc.ca




From daemon Thu Mar 16 17:42:29 2000



From: Rick Harris :      raharris-at-ucdavis.edu
Date: Thu, 16 Mar 2000 09:07:14 -0800
Subject: Re: Multi-user facility managers

Contents Retrieved from Microscopy Listserver Archives
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At 08:22 AM 3/16/00 -0500, Leslie Eibest wrote:
} Hi, folks;
}
} One concern raised by a granting agency about our proposal
} for new equipment for a multi-user SEM lab was our lack of a
} management plan (primarily for time allocation among users and for
} conflict resolution). We have been operating under a first
} come-first served policy, and conflicts have not been a problem.
} I would greatly appreciate insights from managers of
} multi-user facilities of any sort as to your approaches to allocation
} of access to instrumentation, and methods of resolving conflicts
} among researchers.
}


Leslie,

I have been managing a multi-user facility since 1979. First it was sign
up sheets. Now it is a web-based calendar. Have a look at out web site.
This works fine for us. We use the web calendar because it is easier for
people across campus to sign up this way than to trek over to the facility.
Also, I don't have to answer the phone to make their appointments.


Rick A. Harris, Director
Microscopy and Imaging Facility
Section of Molecular and Cellular Biology
1241 Life Sciences Addition
University of California
Davis, CA
530 752 2914
530 754 7536 fax
http://katie.ucdavis.edu
raharris-at-ucdavis.edu



From daemon Thu Mar 16 17:42:30 2000



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Thu, 16 Mar 2000 09:49:32 -0800
Subject: RE: [TEM] Problem with magnetic samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Jose,
One other method that might work for you is the old "window-polishing"
technique. This was the preferred method before the jet-polishing machines
were in wide use. A sheet of the metal about one inch by two inches and as
thin as possible is masked by stopping lacquer on the edges and
electropolished in a bath of electrolyte. When it has polished down to the
"lacey" point, a small protrusion of the metal is sliced off with a razor
blade and sandwiched in a folding grid or between two grids. The edges,
except for the sliced part, are thin enough to examine. It is a bit of an
art and takes some practice to get right, but the resulting piece is small
and securely held.
-----Original Message-----
} From: Jose Maria Manero [mailto:manero-at-cmem.upc.es]
} To: Microscopy-at-sparc5.microscopy.com
} Subject: [TEM] Problem with magnetic samples

} Dear all,

} I want to observe pure iron by means of TEM (Jeol 1200 EX II) in order
} to study the dislocations substructure. But I have some problems, for
} example, I can not focus the image due to the magnetism of the ferrite.

} I am looking for suggestions to avoid this problem. What could I do?

} Thank you in advance.

} Dr. J. M. Manero
} Universidad PolitŽcnica de Catalunya.
} E.T.S.I. Industriales de Barcelona. Spain.
} e-mail: manero-at-cmem.upc.es

Regards,
Mary


Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Thu Mar 16 17:42:32 2000



From: McLean, Dorrance :      dmclea-at-sandia.gov
Date: Thu, 16 Mar 2000 11:42:44 -0700
Subject: RE: Multi-user facility managers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Leslie,
You can post a calendar on your internal web page for users to log onto, or
if you do not have a web page post a calendar at each microscope. Let your
users sign up as far in advance as they can, this eliminates most of the
conflicts and the habitual "last minute guy" learns to get him/herself more
organized. It also gives you a record of use, I also log in all down time
due to maintenance service etc. The best part is this system is really easy
once everyone buys into it.
Good luck!
} ----------
} From: Leslie Eibest
} Sent: Thursday, March 16, 2000 5:22 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Multi-user facility managers
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi, folks;
}
} One concern raised by a granting agency about our proposal
} for new equipment for a multi-user SEM lab was our lack of a
} management plan (primarily for time allocation among users and for
} conflict resolution). We have been operating under a first
} come-first served policy, and conflicts have not been a problem.
} I would greatly appreciate insights from managers of
} multi-user facilities of any sort as to your approaches to allocation
} of access to instrumentation, and methods of resolving conflicts
} among researchers.
}
} Thanks for any information!
}
} Leslie Eibest
} Zoology Dept.
} Duke University
}
}



From daemon Thu Mar 16 17:42:32 2000



From: Larry :      mishot-at-itsa.ucsf.edu
Date: Thu, 16 Mar 2000 10:42:29 -0800
Subject: Multi-user facility

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have modified the basic sign up sheet due to very heavy demand on our
microscope. Except for special cases (eg., time lapse studies, emergencies)
we allow users to sign up for no more than two hour blocks of time and they
cannot sign up for additional time until that block is completed. In
addition, if they are more than 15 minutes late for their time the entire
block of time is forfeited. (This has rarely happened but emphasizes the
need for responsible behaviour.) This system was adapted from the one that
Mei Li Wong uses for electron microscopes.
Larry D. Ackerman
Lily & Yuh Nung Jan Laboratories
Howard Hughes Medical Institute
UCSF, Box 0725, Rm U226
533 Parnassus Ave.
San Francisco, CA 94143

(415) 476-8751 FAX (415) 476-5774
mishot-at-itsa.ucsf.edu



From daemon Thu Mar 16 17:42:33 2000



From: Bruce Brinson :      brinson-at-cnst.rice.edu
Date: Thu, 16 Mar 2000 13:03:32 -0600
Subject: Re: Multi-user facility managers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Leslie,
Yikes! this reeks of infectious administratium but since you need to deal
with the request...
I have quite a few systems that are considered multiple user
facilities. Over the last 9 years I can say I have never had a conflict to
resolve or even heard about one... meaning the users work well together.
Users make reservations on a calendar. That is their time. If the
machine is down, that time is lost. There is not a ripple effect or
compression of the schedule to squeeze them in Frankly if any user asked
another for time due to there state of desperation I have no doubt that they
would work together.
I have a rule for my EMs that only one 4 hour period may be reserved at
a time. I think this keeps the level of respect for someone else's time
high.
My good fortune aside, you could probably get an outline for conflict
resolution from your HR dept. & append it to your proposal.

Bruce Brinson,
Rice U.

Leslie Eibest wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hi, folks;
}
} One concern raised by a granting agency about our proposal
} for new equipment for a multi-user SEM lab was our lack of a
} management plan (primarily for time allocation among users and for
} conflict resolution). We have been operating under a first
} come-first served policy, and conflicts have not been a problem.
} I would greatly appreciate insights from managers of
} multi-user facilities of any sort as to your approaches to allocation
} of access to instrumentation, and methods of resolving conflicts
} among researchers.
}
} Thanks for any information!
}
} Leslie Eibest
} Zoology Dept.
} Duke University



From daemon Thu Mar 16 17:42:35 2000



From: Anastasia Kanellopoulos :      sia-at-sciencewares.com
Date: Thu, 16 Mar 2000 14:45:17 -0500
Subject: SEM/TEM parts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greetings -

We are working on a research project and we need an electron gun with 5-10
kV (wehneldt assembly) and a lens system (for electrons, not photons) that
is typically found in SEM systems. Does anyone have a decommissioned or
un-needed SEM or TEM equipment from which we could get these components at
low or no cost (we are on a very tight budget)? We will even move out a
complete system if it will help someone who needs the space.

Thanks in advance for any help on this!

Sia Karplus
sia-at-sciencewares.com




From daemon Thu Mar 16 17:42:36 2000



From: Earl Weltmer :      earlw-at-pacbell.net
Date: Thu, 16 Mar 2000 11:54:12 -0800
Subject: Re: Amray 1910FE SEM maintenance contract

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listservers,

I recently responded to a thread about Amray FE maintenance contracts.
My response contained two errors: one numerical, one in perception.
I wish to correct them at this time.

First the numerical correction: I stated that the JEOL FE gun exchange
cost was $17,000.00: it is actually $7,000.00.
The $17,000.00 figure was obtained from two JEOL customers which I
presumed to be true as
I am not in the habit to ask other competitors their pricing schedules.

Second the perception error: I stated that " Hitachi is doing well and
doesn't need to worry about "locking" third party maintenance or anyone
else out."
I did not mean to imply that JEOL or any other manufacturer's business
was poor -- just that Hitachi was doing well. I am sure that JEOL's
business is excellent as they have an excellent product.
Moreover, their service organization is one of the best. As their
competitor we have a difficult time competing with JEOL.

Keep in mind that I do not have a "cozy" relationship with Hitachi. I
just respect the technology & the professional co-operation I get from
them & other manufacturer's like them.
I can buy schematics, parts lists, & even service manuals from Hitachi
that other manufacturers don't offer because they consider it
proprietary.
It makes my job easier.

I was using Hitachi as one example because in a pure free enterprise
system the customer is the ultimate winner.

I apologize for the errors. I wish to thank JEOL for pointing out the
error and their gracious attitude in dealing with this error.

Regards,

Earl Weltmer
Scanservice Corporation



From daemon Thu Mar 16 17:42:36 2000



From: Diana_Papoulias-at-usgs.gov (Diana Papoulias)
Date: Thu, 16 Mar 2000 13:52:58 -0700
Subject: accu-edge blades

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Could someone please tell me where I can purchase accu-edge microtome
blades?

Thank you.

Diana Papoulias
USGS
4200 New Haven Rd
Columbia, MO 65201

T:573 876 1902
F:573 876 1876
E:Diana_Papoulias-at-usgs.gov



From daemon Thu Mar 16 17:42:38 2000



From: Ron L'Herault :      lherault-at-bu.edu
Date: Thu, 16 Mar 2000 15:36:09 -0500
Subject: RE: Multi-user facility managers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have a calendar that people fill in. As a courtesy to others, we ask
that they not block more than 4 hours at a time. So far this has worked
well. On the few occasions when another user was in a bind, scheduled
users have been accommodating because they know that they could be in the
same position.

Ron

-----Original Message-----
} From: Leslie Eibest [SMTP:leibest-at-duke.edu]
[} ]
[} ]
I would greatly appreciate insights from managers of
multi-user facilities of any sort as to your approaches to allocation
of access to instrumentation, and methods of resolving conflicts
among researchers.





From daemon Thu Mar 16 17:42:44 2000



From: flaitz-at-us.ibm.com
Date: March 29, 2000
Subject: Meeting: Metropolitan Microscopy Society, Fishkill, NY.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Metropolitan Microscopy Society

Spring Meeting 2000


Time: 8:45 am (registration begins)

Place: IBM Microelectronics, E. Fishkill, NY, West Complex, Bldg 600.

The IBM Corporation has graciously agreed to be our host for this
meeting at their facility in East Fishkill, NY (West Complex, Bldg
600). Attendees will be able to purchase lunch in the adjacent IBM
cafeteria. Due to size and security issues IT IS ESSENTIAL THAT
MEMBERS PRE-REGISTER so that an attendee list can be delivered to the
IBM security folks to prepare guest badges and escorts. DUE TO THE
TIGHT SECURITY CLEARANCE REQUIREMENTS, WALK-INS CANNOT BE
ACCOMMODATED.

THE REGISTRATION DEADLINE IS MARCH 24th AND CAN BE ACCOMPLISHED
ELECTRONICALLY. Please respond via email (or fax) to Evan Slow
directly. A simple email note or a completed fax of the registration
form is all that s required to register. You can then bring the
required fee with you to the meeting. The meeting fee, which does not
include lunch, is $15.00. ON-SITE REGISTRANTS WILL BE CHARGED $25.00
BUT, AGAIN, UNLESS YOU HAVE BEEN PREVIOUSLY CLEARED THROUGH IBM
SECURITY, WE CANNOT ACCOMMODATE YOU.

Registration: Email -- ess-at-feico.com
FAX --- (201) 760-2525

----------------------------------------------------------------------

AGENDA


8:45 - 9:30 : Registration (Coffee)


9:30 - 9:45 : Introductory Remarks (Al Sicignano).


9:45 - 10:45 : CHARACTERIZATION OF MICROSTRUCTURES IN MICROELECTRONIC
INTERCONNECTS, Lynne Gignac, IBM T.J. Watson Research Center.


10:45 - 11:45 : IMAGING, DIFFRACTION AND SPECTROSCOPY WITH FIELD EMISSION
GUN SEM (FEG SEM) AT LOW VOLTAGE, Vinayak Dravid, Northwestern U.


11:45 - 12:45 : DEEP-UV CONFOCAL MICROSCOPY OF SUB-MICRON FEATURES, Mike
Torres, Metron Technology.


12:45 - 1:30 : LUNCH -- available for purchase in the IBM cafeteria.


1:30 - 2:30 : THE USE OF MONTE CARLO CALCULATIONS FOR QUANTITATIVE X-RAY
MICROANALYSIS, Eric Lifshin, GE Corporate Research & Development.


2:30 3:30 : STUDIES OF SAMPLES HAVING SHALLOW SURFACE TOPOGRAPHY BY
THE LOW-LOSS ELECTRON (LLE) METHOD IN THE SCANNING ELECTRON MICROSCOPE
(SEM), Oliver Wells, IBM T. J. Watson Research Center.







From daemon Thu Mar 16 18:10:43 2000



From: Mel Dickson :      m.dickson-at-unsw.edu.au
Date: Fri, 17 Mar 2000 10:55:04 +1100
Subject: Re: Multi-user facility managers

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At 08:22 16/03/00 -0500, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


I have run a first come-first served policy for 30 years. Its the way to go.

It now is self-administering through our equipment booking software.

VERY seldom we need to reason with excessive users. We can almost always
sort problems out with time trading.

Use Internet explorer - Go to http://srv.emunit.unsw.edu.au/ - instrument
booking and use login {guest} password {guest} to get its flavour.


}
}
Dr. Mel Dickson,
Deputy Director, The Electron Microscope Unit,
Adjunct Associate Professor, School of Microbiology & Immunology
The University of New South Wales
UNSW SYDNEY 2052
Australia.
Phone +612 9385 6383 Fax +612 9385 6400



From daemon Thu Mar 16 18:30:49 2000



From: hank adams :      hpadams-at-bcm.tmc.edu
Date: Thu, 16 Mar 2000 18:27:29 -0600
Subject: Multi-user management

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


It helps to have a good management plan in place whether the facility is extremely busy or not. It avoids most conflicts and gives manager/coordinator a set of rules to use that can be applied to any situation that might pop up. You might not that busy currently on conflicts are occuring but later on the situation can quickly change. Granting agencies want to know if the equipment they are funding are accessible and not misused. We are a fairly busy facility and have been using calendars, lined up together, on a wall for each piece of equipment including work stations. We have one for the current week and below that one for the next week users can sign up. Depending on the instrument from two to three hour slots at a time during peak hours. After 6PM and before 8:30AM longer durations are possible. One can only cancel a day in advance on an instrument. No shows are charged. We cross out time slots for instrument maintenance as needed and as much inadvance as possible. Potential users first fill out an investigator profile form before they can cycle into the facility. This helps us target the instrument/technique they need. Based on several factors we prioritize users. All users must take a tutorial and demonstrate competency before they can solo. We do tutorials only one day a week unless there is an important reason that they cannot do it on that day. In a few weeks, I hope, we are going to our web based scheduler in which users have the a couple months to schedule in advance. It will automatically restrict a users' time usage to only peak hours or to peak and after hours depending on their competency, and other criteria. It will prevent users from making cancellations less then the day before. Down times, etc can be easily entered on the calender. The web based schedular will make it allot more convenient for users and core staff. And best of all, please god let it work smoothly, data from the scheduler will flow into our billing data base for semi automatic billing. So far, with the written calendar syst
conflicts, however, usage is so high on some instruments not every user can get the slot they need and so are unhappy campers. In this case, we encourage users, if it is possible, to time their experiments based on when they can get on the instrument.
I hope this helps.

Hank Adams
Lab Manager
Integrated Microscopy Core
Molecular and Cellular Biology
Baylor College of Medicine
Houston, TX



From daemon Thu Mar 16 18:30:51 2000



From: Mel Dickson :      m.dickson-at-unsw.edu.au
Date: Fri, 17 Mar 2000 10:55:04 +1100
Subject: Re: Multi-user facility managers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 08:22 16/03/00 -0500, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


I have run a first come-first served policy for 30 years. Its the way to go.

It now is self-administering through our equipment booking software.

VERY seldom we need to reason with excessive users. We can almost always
sort problems out with time trading.

Use Internet explorer - Go to http://srv.emunit.unsw.edu.au/ - instrument
booking and use login {guest} password {guest} to get its flavour.


}
}
Dr. Mel Dickson,
Deputy Director, The Electron Microscope Unit,
Adjunct Associate Professor, School of Microbiology & Immunology
The University of New South Wales
UNSW SYDNEY 2052
Australia.
Phone +612 9385 6383 Fax +612 9385 6400



From daemon Thu Mar 16 20:11:32 2000



From: Rosemary Walsh :      rw9-at-psu.edu
Date: Thu, 16 Mar 2000 19:57:01 -0600
Subject: Re: Subject: SEM - Problems with marking bacteria

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Christian, I have a few approaches you might consider: a. Where
possible, I usually obtain a culture of the bacteria thought to adhere to a
substrate and prepare it exactly the same way:fix, dehydrate, cpd and
sputter-coat. b. Prepare two samples but after full prep, apply a cellulose
acetate film (or duco cement) to one surface moistened with a drop of
acetone and try to strip the bacteria from the bone cement -- I don't know
about     damage to the methacrylate. This technique
produces a replica of the surface.  I have used it to remove
bacteria colonizing a planchet of hornblende.  We were able to locate
the pits made by the bacteria and image the bacteria removed, i.e.,
stripped from the mineral. c. If the above approach fails to produce the
results you need, try making a replica with double sided C tabs, the type
sold by EMS for X-ray microanalysis. d. If staph epidermidis has a
glycocalyx you might try adding ruthenium red to the GA in the primary fix
and prep as usual but instead of coating with Au or Au/Pd, evap C and use a
backscatter detector to obtain a contrast BE image where the brighter
areas should be the Ru stained bacteria and/or collect a spectrum to
check for the presence of Ru.  If there is a characteristic peak, you
could map the Ru and  localize the bacteria with an x-ray dot map. I
would be interested in knowing how you resolved this problem. Rosemary
Walsh The Electron Microscope Facility for the Life Sciences, A Shared
Technology Facility, The Life Sciences Consortium 1 South FrearLab Penn
State University University Park, PA 16802 (814) 865-0212 rw9-at-psu.edu
{"http://www.lsc.psu.edu/stf/em/home.html"
eudora="autourl"} http://www.lsc.psu.edu/stf/em/home.html




From daemon Thu Mar 16 23:05:51 2000



From: Ford Royer :      froyer-at-bitstream.net
Date: Thu, 16 Mar 2000 22:59:16 -0600
Subject: Re: accu-edge blades

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I sell the DruAedge blades. Same quality as the accu-edge, but at a better
price. Please contact me for pricing and free samples. I carry both the High
Profile and Low Profile Teflon coated blades.

Ford M. Royer
Analytical Instruments, LLC
9921 13th Ave. N.
Minneapolis, MN 55441
phone: (800) 565-1895, ext. 17
fax: (612) 929-1895
froyer-at-bitstream.net

Diana Papoulias wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Could someone please tell me where I can purchase accu-edge microtome
} blades?
}
} Thank you.
}
} Diana Papoulias
} USGS
} 4200 New Haven Rd
} Columbia, MO 65201
}
} T:573 876 1902
} F:573 876 1876
} E:Diana_Papoulias-at-usgs.gov



From daemon Fri Mar 17 06:37:23 2000



From: Molecular Biology Lab - Dept of Botany :      paam-at-rs.uovs.ac.za
Date: Fri, 17 Mar 2000 09:45:10 GMT2
Subject: TEM Immunogold labeling spesificity

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am trying to localise a beta-1,3-glucanase gene after Russian wheat aphid
infestation. However I am getting quite heavy labeling in the chloroplasts and
from the literature this haven't been previously documented.
My control serum shows a small amount of labeling in cell walls but these
seems to be random. The controls (before infestation) shows very little
labeling.
Work done with the same antibody on rust infested wheat plants have shown
no, to very little labeling in the chloroplasts.
My question is how can I make sure this is not artifacts and that the glucanase
are for sure in the chloroplasts.
Martin Wilding
Department Botany & Genetics
University of the Orange Free State
P.O. Box 339
Bloemfontein
9300
South Africa

Tel +2751 4012818
Fax +2751 4488772
Email paam-at-rs.uovs.ac.za



From daemon Fri Mar 17 07:47:26 2000



From: Tom Isabell :      tc_isabell-at-fischione.com
Date: Fri, 17 Mar 2000 08:29:01 -0500
Subject: Open Position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


E.A. Fischione Instruments, Inc. is seeking an individual for the position
of a Sales Engineer.
The successful candidate will be responsible for one or more states from
both inside the office in Export, PA. and in the field. The candidate's
responsibilities will include defining contacts, maintaining information in
the sales database, qualifying leads and determining need, providing
information and quotations for standard products, handling service/spare
parts requests and coordinating the same with the Service Department. Must
develop a thorough technical knowledge of standard products. Extensive
travel required.

The candidate should have an Associates Degree in either Electron
Microscopy technology, Material Science, Engineering, or Physics as a
minimum; a B.S. or M.S. would be preferred.

Salary is commensurate with experience.

E.A. Fischione Instruments, Inc. is an equal opportunity employer. Please
send your resume and salary requirements to:

Human Resources Director, MSA LS
E.A. Fischione Instruments, Inc.
9003 Corporate Circle Export, PA 15632
Phone (724) 325-5444 FAX (724) 325-5443
E-mail: info-at-fischione.com
www.fischione.com



From daemon Fri Mar 17 07:57:26 2000



From: Louis M Kerr :      lkerr-at-mbl.edu
Date: Fri, 17 Mar 2000 08:53:52 -0500
Subject: Re: LM: Fluorescence lamp instability

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


David and others,

This does not directly address the problem with your current lamp but you
might want to consider looking at this new lamp assembly. We recently
evaluated the system and there were no visible stability problems as well
as several advantages over the standard mercury lamp housing.

The company is called EFOS. This unit replaces the standard mercury source
for fluorescence and possibly transmitted light techniques. It uses an
external 50W miniature arc lamp incorporated into an elliptical reflector
which is connected to the microscope by a liquid light guide. Our unit was
mounted on a Zeiss Axioscope.

You can visit the EFOS web site at http://www.efos.com/products/x-cite.htm

Thanks,
Louie


At 2:56 PM -0400 3/15/00, O'Neil, David wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Louie Kerr
Research and Education Support Coordinator
Marine Biological Laboratory
7 MBL Street
Woods Hole, MA 02543
508-289-7273
508-540-6902 (FAX)
508-292-0289 (Cell phone)

VISIT OUR WEB SITE:
http://www.mbl.edu




From daemon Fri Mar 17 17:05:07 2000



From: William Tivol :      tivol-at-wadsworth.org
Date: Fri, 17 Mar 2000 10:46:12 -0500
Subject: Re: Multi-user facility managers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Leslie Eibest wrote:

} I would greatly appreciate insights from managers of
} multi-user facilities of any sort as to your approaches to allocation
} of access to instrumentation, and methods of resolving conflicts
} among researchers.
}

Dear Leslie,
Our facility is funded by NIH as a Biotechnological
Resource, so we deal with both in-house and outside researchers.
Like the other responders, we have not had any conflicts between
users since we started. Because the outside users must spend con-
siderable time arranging to travel here to use the instruments, we
have had a policy which maximizes the utility of their stay here.
We have a staff person whose function is outside user liason;
she ascertains what the user wants, suggests ways to prepare the
specimen to achieve that, examines trial grids, schedules the user,
and stays with the user during the run. For the inexperienced
user, she does almost everything except select the area of the grid
to be photographed; for users who are familiar with our facility,
she changes specimens, develops film, and is available for any
other user needs. For the occasional user who can operate the
HVEM on his own, she still changes the film and is available.
The rest of the staff is responsible for starting the HVEM
up in the mode the user wants, taking care of any problems which
arise, and setting up for non-routine use--EDS, diffraction, etc.
In-house users can be bumped to accomodate outside
users and can only sign up a few weeks in advance. Outside users
can sign up for as far in advance as they wish, and if any problems
arise with the scope, our liason person calls to let them know so
they can change plans if necessary. In periods of very high use,
the staff arranges to keep the scope up for as long as 16 hours a
day and will also arrange to be here after hours and/or on week-
ends to accomodate users' schedules.
Yours,
Bill Tivol



From daemon Fri Mar 17 17:05:07 2000



From: Robert Underwood :      underwoo-at-u.washington.edu
Date: Fri, 17 Mar 2000 08:31:11 -0800 (PST)
Subject: capture speed for image ratios

Contents Retrieved from Microscopy Listserver Archives
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Hello microscopist,

Is there a simple answer to the question:

How fast must the sequential image capture be to perform image ratios for
dtermining something like calcium ion levels? If you have a fast interline
or frame transfer camera, does a single filter wheel like a Ludl change
filters fast enough?

Bob
Morphology Core Lab
U of Washington
Seattle



From daemon Fri Mar 17 17:05:08 2000



From: Beverly_E_Maleeff-at-sbphrd.com
Date: Fri, 17 Mar 2000 11:57:49 -0500
Subject: Job announcement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Cell Biologist/Microscopist

SmithKline Beecham, a world class leader in Research and Development, continues
to pioneer innovative pharmaceutical and healthcare products and services. We
have the following opportunity available at our state-of-the-art suburban
Philadelphia facility. Working in our Safety Assessment department you will
provide technical support and scientific input into the design and execution of
studies to elucidate the cellular mechanisms of drug-induced toxicities. You
will prepare biological specimens for transmission and scanning electron
microscopy, confocal microscopy, X-ray microanalysis and immunohistochemistry.
You will also perform qualitative and quantitative data analysis using computer
assisted image analysis systems. We require a BS/MS in a biological science, and
3-5 years of microscopy experience in cell biology, physiology, toxicology.
SmithKline Beecham is dedicated to an innovative workplace and supports you with
career long opportunities and learning. We offer a competitive benefits and
compensation package. For confidential consideration, please forward your
scannable resume to:
SmithKline Beecham
Attention: Human Resources
AD CODE: 2K0325W
c/o National Resume Processing
P.O. Box 1070
Burlington, MA 01803 USA

Indicating ad code is essential. Principals only, no agencies, please. For a
full listing of current opportunities, or to submit a resume online, visit our
website at www.sb.com/careers.

**********************************************************************************************

Please respond directly to the address in the advertisement, and not to me.

Regards,
Bev Maleeff




From daemon Fri Mar 17 17:05:08 2000



From: sherwood-at-helix.mgh.harvard.edu (Peggy Sherwood)
Date: Fri, 17 Mar 2000 12:03:46 -0500
Subject: Multi-user facility managers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Leslie,
Just to add my two cents: I supervise a private Photopathology Lab for the
Wellman Labs of Photomedicine at MGH. We have an Axiophot (Zeiss)
photomicroscope that has an image analysis system attached. We have used a
monthly sign-up calendar successfully for 10 years. The room is secured--
anyone wishing to use the system, must be checked out first by one of us.
Once we are con-
fident they know what they are doing, they then sign up ahead for a day and
time. We allow after- hour use; many researchers work weekends and nights.
I sign out a lab key to the individual and the room key is in a desk
drawer. People are instructed to make sure the microscope is off, doors
are locked, lights are off and room is secure. Other than the occasional
light left on, the system has worked. The other advantage to having
people sign-up is that if there is a problem with the micro- scope and/or
room, we can go back to the last person who used it and advise them of the
problem.
We also use a sign-up system for a multi-headed teaching scope.

Whatever system you end up using--sign-up sheets or on-line, you have a
record of use, which granting agencies are most interested in: making sure
you get the most for your buck!

Good luck!

Peggy Sherwood
Wellman Labs of Photomedicine-MGH
50 Blossom Street
Boston, MA 02114
617-726-6983 (Photopathology Lab)
617-724-4839 (voice mail)
617-726-3192 (fax)
e-mail: sherwood-at-helix.mgh.harvard.edu






From daemon Fri Mar 17 17:05:08 2000



From: Leslie Eibest :      leibest-at-duke.edu
Date: Fri, 17 Mar 2000 12:23:33 -0500
Subject: Multi-user facility management

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thanks so much to all of you who took the time to respond to
my question. My management techniques are basically the same as most
of yours, but my presentation was woefully inadequate. Thanks to
you, I'm well-armed now!

Leslie Eibest



From daemon Fri Mar 17 17:05:09 2000



From: John C. Wheatley :      John.Wheatley-at-asu.edu
Date: Fri, 17 Mar 2000 10:57:39 -0700
Subject: Multi-user facility

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have had several instruments and many instrument users during each the
last twenty five years. Originally, we used calendar sign-up and log book
entries to compile data for accounting and annual reports. This became too
time-consuming so we developed an in-house automated (computer-based)
sign-up (registration) system in 1990. The system serves as an instrument
reservation unit and an automated accounting system. We recently developed
(continue to develop) a web-based system which allows researchers to
reserve time from their offices or anywhere they can access the internet.
You may wish to view it at the following URL:
http://signu.la.asu.edu:8001/. (Login: guest Password: testit) You will
not be able to view the accounting data. The sign-up program allows only
qualified users to operate an instrument. Also, rules for successive time
reservation are integrated into the program. Optional holders and
spectrometers can be reserved at the time the instrument is signed for.

We presently have 90 research users. In addition, there are approximately
sixty student users (grad and undergraduates) who are taking semester
classes in microscopy or they have one or two sessions on individual
instruments to better understand the role of electron microscopy for
solving problems in Geology, solid state chemistry etc.


Each instrument has a keypad timer interfaced to either the filament or
high voltage on/off switch, depending on ease of accessibility. The timer
is interfaced to a 486 computer (obtained from surplus-there are lots of
these available). The computer stores user time for each account as well
as the number of films exposed for the instrument used. Because we have to
pay for approximately 90% of our operations costs, we have to charge for
use of the instruments. We also charge different hourly rates depending on
user status (inside or outside user).

At the end of each month, the user time/film number data is dumped into
another computer which compares the reserved time from the web-based
reservation system to the actual use time and charges for the greater of
the two. If the instrument develops problems during the use time and can't
be used any more, the individual user is able to send a DOWN message to the
reservation computer and the user is charged only for actual time used. The
monthly use data is formatted into a spreadsheet which contains microscope
use times and film numbers used. A principal investigator (PI) may have
ten grad students who use five different instruments and three different
grant account numbers during the month. He/she receives a statement at the
end of the month which identifies the microscope user by name, the account
number used, the hours used (rounded to the nearest minute)for each
instrument and the total number of films exposed for each use time. The
total costs for instrument use is given on the last line of the statement.

An advantage of this system is that within thirty minutes, one can sort
data files and determine how many hours each microscope is used over any
period of time; how many hours each user utilizes an instrument(s) over
time; number of hours used for each account and total number of hours for
all instruments over time. This info is useful for reports to
administrators as well as for funding agency applications.

If you have questions about our system, please e-mail me directly or call
at the number listed below.

John C. Wheatley
Lab Manager
Arizona State University
Center for Solid State Science
PSA-213
BOX 871704
Tempe, AZ 85287-1704


Phone: (480) 965-3831
FAX: (480) 965-9004
John.Wheatley-at-ASU.Edu




From daemon Fri Mar 17 17:05:11 2000



From: John C. Wheatley :      John.Wheatley-at-asu.edu
Date: Fri, 17 Mar 2000 13:34:08 -0700
Subject: Multi-user facility--correction

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I forgot to put the p in signup for the URL in the message I sent out this
morning. Thanks to David Kenriks for catching it. The right URL is:

http://signup.la.asu.edu:8001/

John C. Wheatley
Lab Manager
Arizona State University
Center for Solid State Science
PSA-213
BOX 871704
Tempe, AZ 85287-1704


Phone: (480) 965-3831
FAX: (480) 965-9004
John.Wheatley-at-ASU.Edu




From daemon Fri Mar 17 17:05:11 2000



From: Wil Bigelow :      bigelow-at-engin.umich.edu
Date: Fri, 17 Mar 2000 15:33:12 -0400
Subject: RCA EMs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all:

A short time ago there was a discussion about the different EMs RCA
produced and when they were produced. The other day I was digging through
my sfiles and came across a copy of the August 1967 issue of the RCA
Scientific Instruments News which has pictures of all the models produced
and the year of introduction:

EMA 1939 I don't think this model was ever sold commercially.
I believe it was built in the RCA Labs for developmental uses

EMB 1940 This was the first commercial model

EMC 1944 This model had a horizontal column

EMT 1950 This was an inexpensive table model that was designed with
use in high schools and small colleges in mind.. As I recall
it had lenses made of permenant magnets to eliminate the need
for lens power supplies and to hold the cost down.

EMU 1944 This was probably RCA's most popular model. It was on their
leading EM for at least 10 years.

EMD 1948 This was an electron diffraction instrument, designed
specifically for obtaining ED patterns by the reflection from
solid surfaces at a grazing incidence.

EML 1954 This was the forerunner of the popular EM-U# and EM-U4 models,
but provided an accelerating voltage of only 50kV. It was
the model in which RCA pioneered the modern ergonomic layout
of controls, with a desk-like design.

EMU-3 1955 Similar in appearance to the EML, but providing 100 kV.

EMU-4 1966 A refinement of the EMU-3, providing a double condenser lens,
a specimen stage airlock system, etc.

RCA ceased marketing electron microscopes in 1969. I was President of
the EMSA that year and discussed the matter with James Hillier, one of the
pioneers in the field of electron microscopy, and a Vice President of RCA
at that time. He said that it was determined that RCA could make more
money selling records and related electronic devices than EMs, and so was
phasing out of the EM business.

The issue of RCA Scientific Instruments News in question also has a picture
of each model on the cover. I have had a copy run off in jpg format. I
understand that it is not appropriate to transmit such info via this list
server; however, if anyone would like a copy, let me know and I'll send it
to you individually.

Happy St. Patrick's Day,

Wilbur C. Bigelow, Prof. Emeritus
Materials Sci. & Engr., University of Michigan
Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu;
Fx:734-763-4788; Ph:734-662-5237




From daemon Fri Mar 17 17:05:11 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 17 Mar 2000 12:51:25 -0600
Subject: SEM maintenance, et. al. (semi-long)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 01:54 PM 3/16/00 , you wrote:

} Dear Listservers,
}
} I recently responded to a thread about Amray FE maintenance contracts.
} My response contained two errors: one numerical, one in perception.
} I wish to correct them at this time.

[snipped for brevity sake]

Thanks Earl for the update and correction. This is good info. And
I see some room for additional discussion and inquiry to wrap up this
topic.

The prospects for third party maintenance contracts are rather
variable--depending mostly, I think, on what is being maintained
under contract. This would be based on the availability of schematics,
software, special parts, etc., etc. If some FESEM makers do not
"guard" their FE guns relative to others, then of course, that will weigh
heavily on third party options. For the record, here are some figures
for Amray maintenance contracts. An 1830 LaB6 system runs
$13,500 annually. This excludes "consumables" such as apertures,
LaB6 cathode, pump oil, etc. But it does include on-site service,
troubleshooting, travel, lodging, meals, rental car, things that
break down, like Balzers 240 turbo pump or Edwards E2M8 mechanical
pump, or the invariable broken wire. Actually, I have found the
1830 to be very reliable and a good performer.

The 1910FE runs $15,000 annually. Same terms as the 1830 but
includes the computer control system. What I don't know is whether
Amray will sell the FE gun assemblies to third party maintainers. I do
know that for Amray users who are not under an Amray contract, the
305FE gun costs about $14,000 total to replace as a per-diem item.
Again, I have found that this SEM also is very reliable. But what
about performance?

Performance wise, I suppose we could have an "image shoot off"
contest based on a standard specimen and different SEM
instruments of different models and from different makers. But there
are some basic differences among SEMs that would otherwise
seem to be the same--but are not. Notable among these differences,
for example, is the design and construction of the final lens pole piece. This is
due, I think, to two or three driving forces. One is the need to image physically
large specimens at various WDs. This means that one needs a large chamber
with a stage that offers accommodation of large samples and wide WD
range. The second force is the requirement to image whole semiconductor
wafers ranging in diameters from 4" to 8" and to have a wide travel
distance across the wafer and to operate well at low KV (photo resist examination,
for example). The third force is the impact on the SEM when needing to
do X-ray analysis.

The flat lens design is quite useable for large and small specimens when
high tilt angles do not increase the WD to an unsatisfactory figure. In the
case of IC wafers, the conical lens is required. These are available as 45
or 60 degree designs. Since most semiconductor qualification imaging
is done at either zero or 45 degrees, the 60 degree lens is probably
optimal. What Amray has done in regards to conical lenses is an
evolutionary path. The 1830 has a conical lens to accommodate high
tilt angles when working with wafers. It also can be configured with a
large chamber. However, the design of the lens is such that high
resolution is obtained at 10KV-15KV and above. It is not a high
resolution SEM at low KV. Alternatively, with a 25KV or 30KV power
supply, it has ample voltage to support the beam currents needed
for x-ray work. I have no personal information on what the other
makers have done and currently offer in regards to IC wafer inspection
and analysis.

The Amray 3600LEAP was a major design departure. This system
also has a 60 degree conical lens, a huge chamber but a specially
designed lens and other features key to semiconductor work. The
LEAP lens was designed for low KV imaging. At 2-3KV, the LEAP
is virtually identical in resolution to the other lens types at 15KV
and at the same WD. This is a key feature and requirement for
imaging fine pitch and small feature size wafers and photoresist.
The other important feature of the 3600LEAP SEM is the 5-place
heated final aperture holder. Since the 3600 runs with 35u and
70u apertures (or less) as routine, these Pt apertures are
continuously heated to keep the effects of contamination
(photo resist again) at a minimum. Not only does this keep the
apertures clean, but it also prevents accumulation of PR in the
scan coil liner. This FESEM is a very capable instrument from
my experience with it. It can easily image 0.15u wide gate poly
at high resolution and 2.5KV at WD=6mm.

If one considers where I have wound up with this discussion,
it is cause for pause to ask a fundamental question. That is, "What
is a research SEM?" Different applications must surely lead
one to select one type of instrument over another. Does it also
direct a path to one manufacturer over another? Since I have
not seen the myriad number of SEM models from all of the vendors,
I cannot answer this question. But while there might be some
disdain for one maker versus others, it seems tough for me to
make any sort of quantitative decision when there are quite a
few variables involved--solely based on application, much less
vendors and maintenance options.

What do you think about this, based on your experience?

gary g.









From daemon Fri Mar 17 17:05:12 2000



From: Nathan Haese :      nathan_haese-at-CompuServe.COM
Date: Fri, 17 Mar 2000 17:22:00 -0500
Subject: SAS Dinner Mtg 4/12 in Mountain View, CA on Raman of Polymer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


TOUR SPEAKER (Society for Applied Spectroscopy) - 4/12/2000 Meeting in
Mountain View

"RAMAN SPECTROSCOPY AS A PROBE OF STRUCTURE/ORIENTATION IN POLYMERIC
FIBERS"

by DR. BRUCE CHASE (DuPont Central Research, Wilmington DE)

at Michaels at Shoreline, Mountain View (Directions to follow)

Register 6:45 PM
Dinner 7:30 PM
Talk 8:30 PM

Cost: $30 for dinner ($10 students and teachers), free for talk.

Dinner Choices: (Indicate Choice when RSVP'ing)

Breast of Chicken, Piccata
Chilean Sea Bass, Ginger Shallot Sauce
Grilled Vegetable Brochette with Wild Rice

Reserve by April 10 to Steve Rabin, 650-564-5315 or steve.rabin-at-alza.com

(Please let us know if you're coming for dinner, or just the talk for
headcount purposes)

Also, see our web site at www.sas-ncss.org

ABSTRACT
Polymeric macroscopic properties such as tenacity, modulus, elasticity,
etc. are determined by molecular level effects such as conformational state
populations, orientation, and intermolecular interactions. Significant
changes occur at the molecular level as a molten or solution phase polymer
is spun into fiber form. The polymer goes from an unoriented, amorphous
state to an oriented, semi-crystalline material via the deformations
imposed by spinning and drawing. Raman spectroscopy can potentially
provide information on both structure and orientation. Changes in band
intensities can be related to conformational populations and to formation
of crystalline regions. Changes in relative intensities as a function of
incident and scattered polarization yields information on chain
orientation. The use of polarized Raman scattering done in both 90 and 180
degree scattering geometries has been used to determine the second and
fourth order orientation functions for both polyethylene and PET fibers.
Raman measurements have been made in both the laboratory frame and on-line
for 66 nylon and PET fibers. For both PET and 66 nylon, crystallinity
levels and a qualitative measure of orientation can be obtained.

DIRECTIONS to Michaels (in Shoreline Park, Mountain View):

} From Anywhere in the Bay Area:

Get to Highway 101 toward Mountain View (from SF and the Peninsula,
southbound; from San Jose and the East Bay via 237, northbound)

Go to the Shoreline Rd. exit and turn left at the end of the exit road.
Follow Shoreline Rd. into (approx 1 mile) through Mountain View Park gate.
Continue on the single lane road (golf course on your left) for approx 1
mile, turn left at Michaels restaurant sign into the parking lot.



From daemon Sat Mar 18 08:09:09 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 17 Mar 2000 18:57:06 -0600
Subject: RE: Fluorescence lamp instability

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Following up on the Hg burner problems, does anyone have
a schematic of the "transformer" for these? There is assumed
to be a true transformer plus a rectifier and filter. Without
a circuit diagram, there is too much speculation for me about
what is actually going on. It seems that there are some common
problems out there. These ought to be readily solvable.

It really cannot be that difficult, can it? Maybe so.

gary g.



From daemon Sat Mar 18 08:09:10 2000



From: Jeff & Wanda Gray :      jefwan-at-flash.net
Date: Fri, 17 Mar 2000 21:12:49 -0600
Subject: accu-edge blades

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Diana,
These blades are available from Allegiance Scientific (used to be Baxter,
used to be Scientific Products, used to be....). As far as I know, this is
the only place that carries this brand.
I have no financial interest in Allegiance, just passing along a fact.
Wanda Shotsberger
(HT ASCP)

-----Original Message-----
} From: Diana Papoulias [mailto:Diana_Papoulias-at-usgs.gov]
Sent: Thursday, March 16, 2000 2:53 PM
To: microscopy-at-sparc5.microscopy.com


Could someone please tell me where I can purchase accu-edge microtome
blades?

Thank you.

Diana Papoulias
USGS
4200 New Haven Rd
Columbia, MO 65201

T:573 876 1902
F:573 876 1876
E:Diana_Papoulias-at-usgs.gov




From daemon Sat Mar 18 08:09:12 2000



From: COURYHOUSE-at-aol.com
Date: Fri, 17 Mar 2000 23:35:44 EST
Subject: Re: RCA EMs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I would like to get hold of an RCA model EMT for display for our museum
collection.
Ed Sharpe archivist for SMECC


{ { Subj: RCA EMs
Date: 3/17/00 2:47:16 PM Pacific Standard Time
From: bigelow-at-engin.umich.edu (Wil Bigelow)
To: AAmy-at-dtsc.ca.gov, JRowe6427-at-aol.com, cgarber-at-2spi.com (Chuck Garber),
john.mardinly-at-intel.com, microscopy-at-sparc5.microscopy.com (Microscopy
Listserver), oshel-at-shout.net

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Hi all:

A short time ago there was a discussion about the different EMs RCA
produced and when they were produced. The other day I was digging through
my sfiles and came across a copy of the August 1967 issue of the RCA
Scientific Instruments News which has pictures of all the models produced
and the year of introduction:

EMA 1939 I don't think this model was ever sold commercially.
I believe it was built in the RCA Labs for developmental uses

EMB 1940 This was the first commercial model

EMC 1944 This model had a horizontal column

EMT 1950 This was an inexpensive table model that was designed with
use in high schools and small colleges in mind.. As I recall
it had lenses made of permenant magnets to eliminate the need
for lens power supplies and to hold the cost down.

EMU 1944 This was probably RCA's most popular model. It was on their
leading EM for at least 10 years.

EMD 1948 This was an electron diffraction instrument, designed
specifically for obtaining ED patterns by the reflection from
solid surfaces at a grazing incidence.

EML 1954 This was the forerunner of the popular EM-U# and EM-U4 models,
but provided an accelerating voltage of only 50kV. It was
the model in which RCA pioneered the modern ergonomic layout
of controls, with a desk-like design.

EMU-3 1955 Similar in appearance to the EML, but providing 100 kV.

EMU-4 1966 A refinement of the EMU-3, providing a double condenser lens,
a specimen stage airlock system, etc.

RCA ceased marketing electron microscopes in 1969. I was President of
the EMSA that year and discussed the matter with James Hillier, one of the
pioneers in the field of electron microscopy, and a Vice President of RCA
at that time. He said that it was determined that RCA could make more
money selling records and related electronic devices than EMs, and so was
phasing out of the EM business.

The issue of RCA Scientific Instruments News in question also has a picture
of each model on the cover. I have had a copy run off in jpg format. I
understand that it is not appropriate to transmit such info via this list
server; however, if anyone would like a copy, let me know and I'll send it
to you individually.

Happy St. Patrick's Day,

Wilbur C. Bigelow, Prof. Emeritus
Materials Sci. & Engr., University of Michigan
Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu;
Fx:734-763-4788; Ph:734-662-5237

} }



From daemon Sat Mar 18 08:09:16 2000



From: mangesh bagade :      mbagade-at-usa.net
Date: 18 Mar 00 02:36:54 MST
Subject: Information of deposition study of chromium on tungsten.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Dear microscopist,
Kindly give me information and websites available on adsorption, desorption
and diffusion study of Chromium on Tungsten by field emission and field ion
microscopy. Also nucleation and growth of Chromium on Tungsten.
Thanking you,




Mangesh Bagade
Govt College Of Engineering Pune
Maharashtra, India.

____________________________________________________________________
Get free email and a permanent address at http://www.netaddress.com/?N=1



From daemon Sat Mar 18 08:09:17 2000



From: mangesh bagade :      mbagade-at-usa.net
Date: 18 Mar 00 02:37:37 MST
Subject: Information of deposition study of chromium on tungsten.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html
by ultra5.microscopy.com (8.9.1b+Sun/8.9.1) id DAA13628
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Dear microscopist,
Kindly give me information and websites available on adsorption, desorption
and diffusion study of Chromium on Tungsten by field emission and field ion
microscopy. Also nucleation and growth of Chromium on Tungsten.
Thanking you,




Mangesh Bagade
Govt College Of Engineering Pune
Maharashtra, India.

____________________________________________________________________
Get free email and a permanent address at http://www.netaddress.com/?N=1



From daemon Sat Mar 18 14:38:50 2000



From: Earl Weltmer :      earlw-at-pacbell.net
Date: Sat, 18 Mar 2000 11:33:48 -0800
Subject: SEM maintenance, et. al. (this one is long)

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H i Gary,

Your e-mail poses some interesting question & perspectives.

As far as the variation of Third Party maintenance contracts,
Scanservice generally offers contracts that are exactly the same as the
given manufacturer.
The customer can then compare "apples against apples". Whenever there is
a question regarding coverage we always look to see what the
manufacturer's contract covers. The variation in contracts is only
between the different manufacturer's. Cambridge (LEO) did not cover
scan coils, ETEC did not cover rotary pumps or CRTs, etc. This
eliminates the confusion for our customers. We can and do offer modified
contracts where the customer is responsible for parts or a limited
number of service calls but only at the customer's request.

All manufacturer's , SEM & otherwise, are required by Federal Law to
sell parts to third party organizations. To withhold parts is considered
a "restriction of trade" and has some hefty consequences. I have had
accounts set-up for just about all the manufacturers. It is relatively
easy process & most manufacturer's treat us like any other customer in
need of parts.

The only exception is Amray that has a multi-step process for obtaining
parts which is as follows:

1. Request part number.
2. Wait two to three days for part number.
3. Send credit references.
4. Wait two to three days for response.
5. Send banking references & information.
6.Wait two to three days for response.

The above process has occurred at least three times in the last three
years. Finally, I requested that they send the parts COD or I could
prepay.
The response I got was that I "could have done that all along."

Bottom line: manufacturer's must sell to Third party organizations, most
are very professional & co-operative.

As far as an "image shoot-off", I really don' think that would be very
practical as there are significant variations even between the same
model from a given manufacturer. I would expect the best performing SEM
from each manufacturer involved, rather than an average of the industry.

When I worked at ETEC, we noticed differences between the different SEM
columns. The demo unit always got the best column. Even today I
recommend that customers purchase the "demo unit" as it probably has the
best of everything & is tuned up. Moreover the "demo unit" can be sold
at a lesser price as it is technically used. I also recommend that
customers considering an SEM purchase use the model they are considering
at another customer's site. In this way, the potential customer can
evaluate the SEM model on their own terms using their own standards and
without the applications engineer's influence. Most manucfacturer's want
to use the standard "gold on carbon" to highlight their given SEM. I
generally do not see many of my customers imaging "gold on carbon" on a
daily basis. Customers has such a variety of samples: photo resist,
uncoated teflon, etc.

This reminds me of a customer at Hughes who I recommended that she
follow the above procedure of evaluating the SEM at a customer site &
requesting that the demo unit be sold to her Company. She did neither.
When she requested that the demo unit be sold to her Company, the
manufacturer declined. No reason was given. She bought the FESEM anyway.
After about three months I inquired about the status of her new FESEM.
Instead of excitement she responded, "Oh it's OK. I guess I will just
have to get used to it". She continued to use her standard tungsten SEM
over the FESEM.

As far as your analysis of the SEM chambers & polepieces, historically
the SEM industry has seen the large chamber sizes and conical polepieces
you decribe for at least last twenty years. When I entered this
industry in 1973, I remember the JEOL JSM-2 with a semi-conical lens.
The lens was not a true conical lens as it did have a "flat tip" of
about 4 cm.dia. but it did allow one to tilt samples at a higher angle.
I was surprised to see JEOL had a JSM-1 (circa 1969) that had the same
column as the JSM-2.

ISI had a model DS-130 that could be ordered with different final
lenses: the standard semi-conical lens, wide bore (WB) for 0 working
distances, or conical lens. The ISI conical lens had multiple angles on
their design. It started with a 30 degree cone, the mid-section was 45
degree, and ended with a 70 degree cone. For it's day the DS-130 had
pretty good optics. The electronics were junk in my opinion. The DS-130
had some extremly large chambers. One was known by the ISI engineers as
a "turkey baster" chamber. It measured about 24 in. x 36 in. x 18 in
high. Hitachi has had the S-806 and S-808 for semiconductor work. An
full 8-inch wafer could be viewed as well as tilted 45 degrees. Hitachi
also offerred an S-806C which is a conical lens. I believe the vintage
for these machines was in the early eighties.

The JEOL JSM-U3 (circa 1970) had a heated final aperture strip that
allowed the user to clean the aperture without removing it. This
aperture design was replaced in the JEOL 35U and JEOL 35C (circa 1974?).
The JEOL 35 series had a continuously heated final aperture. A smaller
current (about 2 amps) was run through the aperture therby cleaning the
aperture while using it.

I recently (last year) had the opportunity to attend a friend's/customer
retirement party. Attending were many older & retired SEM engineers.
They were reminiscing about the SEM industry and how the machine had
evolved from their days at Westinghouse (yes Westinghouse) in the
1960's. The same questions and problems that we have today they had in
the 1960's. Chamber sizes were initially large to accomodate their
sample and stage sizes but vacuum was poor (10-4 torr). Chamber sizes
were reduced to improve vacuum and lens designs were changed to
accomodate tilt angles at the expense of resolution. And everyone could
only dream of a stable FE gun. SEM nerds.

The upshot of all of this is that all of these issues has been
historically addressed. Large & small chambers, different lenses, etc.
will be with us as long as the application permits. When a manufacturer
claims to have a "breakthough", it only remind me of what Steve Jobs
(Apple Computer) said about Windows 95: " Windows 95: MacIntosh 1986." I
think the next real breakthrough will be the lenses made from
super-conductive wire or, better yet, the electrostatic columns being
developed.

It is my understanding that the electrostatic columns are Shotky FE
sources with backscatter dectectors fully integrated onto the "final
lens". Future features include an integrated EDS detector. The columns
are small & cheap enough to be "thrown away".

I really don't have a disdain for any given Company. I would only like
to comment that every Company has a different "flavor" depending upon
whom is running it. Most are a pleasure to work with, while others leave
a "bad taste in your mouth".

Please don't take these comments personally as I respect your opinion &
have throughly enjoyed the subjects you have brought up on this
listserve & look forward to future your comments.

Regards,

Earl Weltmer



At 01:54 PM 3/16/00 , you wrote:

} Dear Listservers,
}
} I recently responded to a thread about Amray FE maintenance contracts.
} My response contained two errors: one numerical, one in perception.
} I wish to correct them at this time.

[snipped for brevity sake]

Thanks Earl for the update and correction. This is good info. And
I see some room for additional discussion and inquiry to wrap up this
topic.

The prospects for third party maintenance contracts are rather
variable--depending mostly, I think, on what is being maintained
under contract. This would be based on the availability of schematics,
software, special parts, etc., etc. If some FESEM makers do not
"guard" their FE guns relative to others, then of course, that will
weigh
heavily on third party options. For the record, here are some figures
for Amray maintenance contracts. An 1830 LaB6 system runs
$13,500 annually. This excludes "consumables" such as apertures,
LaB6 cathode, pump oil, etc. But it does include on-site service,
troubleshooting, travel, lodging, meals, rental car, things that
break down, like Balzers 240 turbo pump or Edwards E2M8 mechanical
pump, or the invariable broken wire. Actually, I have found the
1830 to be very reliable and a good performer.

The 1910FE runs $15,000 annually. Same terms as the 1830 but
includes the computer control system. What I don't know is whether
Amray will sell the FE gun assemblies to third party maintainers. I do
know that for Amray users who are not under an Amray contract, the
305FE gun costs about $14,000 total to replace as a per-diem item.
Again, I have found that this SEM also is very reliable. But what
about performance?

Performance wise, I suppose we could have an "image shoot off"
contest based on a standard specimen and different SEM
instruments of different models and from different makers. But there
are some basic differences among SEMs that would otherwise
seem to be the same--but are not. Notable among these differences,
for example, is the design and construction of the final lens pole
piece. This is
due, I think, to two or three driving forces. One is the need to image
physically
large specimens at various WDs. This means that one needs a large
chamber
with a stage that offers accommodation of large samples and wide WD
range. The second force is the requirement to image whole semiconductor

wafers ranging in diameters from 4" to 8" and to have a wide travel
distance across the wafer and to operate well at low KV (photo resist
examination,
for example). The third force is the impact on the SEM when needing to
do X-ray analysis.

The flat lens design is quite useable for large and small specimens when

high tilt angles do not increase the WD to an unsatisfactory figure. In
the
case of IC wafers, the conical lens is required. These are available as
45
or 60 degree designs. Since most semiconductor qualification imaging
is done at either zero or 45 degrees, the 60 degree lens is probably
optimal. What Amray has done in regards to conical lenses is an
evolutionary path. The 1830 has a conical lens to accommodate high
tilt angles when working with wafers. It also can be configured with a
large chamber. However, the design of the lens is such that high
resolution is obtained at 10KV-15KV and above. It is not a high
resolution SEM at low KV. Alternatively, with a 25KV or 30KV power
supply, it has ample voltage to support the beam currents needed
for x-ray work. I have no personal information on what the other
makers have done and currently offer in regards to IC wafer inspection
and analysis.

The Amray 3600LEAP was a major design departure. This system
also has a 60 degree conical lens, a huge chamber but a specially
designed lens and other features key to semiconductor work. The
LEAP lens was designed for low KV imaging. At 2-3KV, the LEAP
is virtually identical in resolution to the other lens types at 15KV
and at the same WD. This is a key feature and requirement for
imaging fine pitch and small feature size wafers and photoresist.
The other important feature of the 3600LEAP SEM is the 5-place
heated final aperture holder. Since the 3600 runs with 35u and
70u apertures (or less) as routine, these Pt apertures are
continuously heated to keep the effects of contamination
(photo resist again) at a minimum. Not only does this keep the
apertures clean, but it also prevents accumulation of PR in the
scan coil liner. This FESEM is a very capable instrument from
my experience with it. It can easily image 0.15u wide gate poly
at high resolution and 2.5KV at WD=6mm.

If one considers where I have wound up with this discussion,
it is cause for pause to ask a fundamental question. That is, "What
is a research SEM?" Different applications must surely lead
one to select one type of instrument over another. Does it also
direct a path to one manufacturer over another? Since I have
not seen the myriad number of SEM models from all of the vendors,
I cannot answer this question. But while there might be some
disdain for one maker versus others, it seems tough for me to
make any sort of quantitative decision when there are quite a
few variables involved--solely based on application, much less
vendors and maintenance options.

What do you think about this, based on your experience?

gary g.



From daemon Sun Mar 19 16:09:40 2000



From: Barbara Foster :      mme-at-map.com
Date: Sun, 19 Mar 2000 16:10:02 -0500
Subject: Re: Surface analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Mr. Albek,

If you are looking for the traces of the tools which were used to make
these surfaces, sounds like a job for an interferometer. There are
several small ones which can be used for low to medium power (10x-50x
objective mag) work. Hach used to carry a small Michelson; also, check
with Nikon for both Michelson and Tolansky varieties. While the Tolansky
is a contact method system, it is non-destructive and, since it is a
multiple beam system, gives very high precision results. I would recommend
photographing the interferograms then correlating with the "tooth marks"
left by various tools. The old Polyvar also had an interferometer module.

For a more upscale, automated version, I'd suggest that you find a lab with
either a Zygo New View or a Wyko RST. These devices are Scanning White
Light Interferometers (SWLIs); both are automated, 3D imaging systems which
might provide interesting insight but may also be overkill for your type of
project. Zygo is just south of us, in central Connecticut, while Wyko is
in Tucson. I believe that both have contract labs. (I have done some work
in the Zygo facility)

Please contact me if you are interested in pursuing this approach.

Best regards,
Barbara Foster
Consortium President
Microscopy/Microscopy Education ...Educating microscopists for greater
productivity.

125 Paridon Street Suite 102 Springfield, MA 01118
PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com
Visit our web site {http://www.MME-Microscopy.com/education}

At 07:29 PM 12/12/99 +0100, S¿ren Albek wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Sun Mar 19 16:09:41 2000



From: Barbara Foster :      mme-at-map.com
Date: Sun, 19 Mar 2000 16:19:28 -0500
Subject: Re: Quantifying the sharpness of glass knives

Contents Retrieved from Microscopy Listserver Archives
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Dear Rosemary,

A client of ours makes a cut in a polymer block (I can check on the exact
material, if you need) then looks at two parameters:
1) the angle made by the sides
2) the radius of curvature of the bottom

He uses a conventional image analysis system for both, using just the
simple angle measurement function for the first and the diameter of a
circle of best fit for the second. He had been using a stereo microscope
but we took a look at the cut under a 10x objective/compound microscope on
a recent visit and found that there was a lot to be learned about the
quality of the cut from the shards and shredding left on the surface (I
can't remember whether it was the upper or lower, so take a look at both).

Hope this is helpful.

Best regards,
Barbara Foster
Consortium President
Microscopy/Microscopy Education ...Educating microscopists for greater
productivity.

125 Paridon Street Suite 102 Springfield, MA 01118
PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com
Visit our web site {http://www.MME-Microscopy.com/education}

At 07:04 PM 3/13/00 -0400, Rosemary Walsh wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Sun Mar 19 16:09:41 2000



From: Barbara Foster :      mme-at-map.com
Date: Sun, 19 Mar 2000 16:25:03 -0500
Subject: Re: Confocal Microscopy in North West Ct

Contents Retrieved from Microscopy Listserver Archives
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Ric,

I think you must be referring to the famous Bill Miller, who was my
"partner in crime" at Sarastro. You can reach him at Bill Miller
{microbill-at-mohawk.net} .

Best regards,
Barbara Foster
Consortium President
Microscopy/Microscopy Education ...Educating microscopists for greater
productivity.

125 Paridon Street Suite 102 Springfield, MA 01118
PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com
Visit our web site {http://www.MME-Microscopy.com/education}



At 11:12 AM 3/14/00 -0500, Ric Felten wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Sun Mar 19 16:09:41 2000



From: Barbara Foster :      mme-at-map.com
Date: Sun, 19 Mar 2000 16:58:43 -0500
Subject: Re: Raman microscopy? was Re: water tracer dye?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

Raman is going to become as available as regular confocal. As a matter of
fact, it is on the same price scale but can do both confocal and chemical
imaging. It is an analytical technique which works well alongside
fluorescence, but you need to collect the Raman signal separately. I just
came back from PITTCON (THE major analytical chemical meeting) and some
companies are collecting the signal in the near UV, others in the near IR.

A good starting point is Jack Koenig's book "Spectroscopy of Polymers" (Am.
Chem. Soc.. Washington DC, 1992). I just saw him at PITTCON and he said
that the new edition is either just coming out or about to come out.

The move to Raman is part of the merging of microscopy and spectroscopy.
There are now several interesting true hybrid instruments which permit
chemical as well as microscopy imaging. One is the Continuum from
Spectra-Tech (Shelton, CT); the other is a series built on the DuraScope
from SensIR (Danbury, CT). I had a chance to teach a microscopy class to
the IR specialists from SpectraTech in January, so really had a chance to
have my hands on the system. In addition to running full FT-IR chemical
spectra, I was able to do low power darkfield. They also have regular
phase and DIC objectives available (the Phase was a bit tricky to
implement) and have just introduced a fluorescence module.

I will be doing a review for American Lab (Watch for Am Lab: "Focus on
Microscopy") and will post pertinent excerpts for you within the next few
weeks.

Best regards,
Barbara Foster
Consortium President
Microscopy/Microscopy Education ...Educating microscopists for greater
productivity.

125 Paridon Street Suite 102 Springfield, MA 01118
PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com
Visit our web site {http://www.MME-Microscopy.com/education}




At 03:00 AM 3/2/00 +0000, Jose Feijo wrote:
} Since the subject was raised, could anyone point me out a good paper or
internet source to understand Raman microscopy, and how to make it work? On
the side, from the gurus, how much should we expect from it in the future?
Is it like, it's going to substitute something already available, or
otherwise it will complement specific aspects of visualisation of some
special biological structure? What does it involve, special lasers, special
optics, special computers?
}
} Thanks in advance
} Jose
}
}



From daemon Sun Mar 19 16:39:44 2000



From: Barbara Foster :      mme-at-map.com
Date: Sun, 19 Mar 2000 17:25:59 -0500
Subject: Re: Raman microscopy? was Re: water tracer dye?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

A propos of our earlier discussion, this just came across the wire from the
Society for Appl. Spectroscopy:

TOUR SPEAKER (Society for Applied Spectroscopy) - 4/12/2000 Meeting in
Mountain View

"RAMAN SPECTROSCOPY AS A PROBE OF STRUCTURE/ORIENTATION IN POLYMERIC
FIBERS"

by DR. BRUCE CHASE (DuPont Central Research, Wilmington DE)

at Michaels at Shoreline, Mountain View (Directions to follow)

Register 6:45 PM
Dinner 7:30 PM
Talk 8:30 PM

Cost: $30 for dinner ($10 students and teachers), free for talk.

Dinner Choices: (Indicate Choice when RSVP'ing)

Breast of Chicken, Piccata
Chilean Sea Bass, Ginger Shallot Sauce
Grilled Vegetable Brochette with Wild Rice

Reserve by April 10 to Steve Rabin, 650-564-5315 or steve.rabin-at-alza.com




Also, see our web site at www.sas-ncss.org

ABSTRACT
Polymeric macroscopic properties such as tenacity, modulus, elasticity,
etc. are determined by molecular level effects such as conformational state
populations, orientation, and intermolecular interactions. Significant
changes occur at the molecular level as a molten or solution phase polymer
is spun into fiber form. The polymer goes from an unoriented, amorphous
state to an oriented, semi-crystalline material via the deformations
imposed by spinning and drawing. Raman spectroscopy can potentially
provide information on both structure and orientation. Changes in band
intensities can be related to conformational populations and to formation
of crystalline regions. Changes in relative intensities as a function of
incident and scattered polarization yields information on chain
orientation. The use of polarized Raman scattering done in both 90 and 180
degree scattering geometries has been used to determine the second and
fourth order orientation functions for both polyethylene and PET fibers.
Raman measurements have been made in both the laboratory frame and on-line
for 66 nylon and PET fibers. For both PET and 66 nylon, crystallinity
levels and a qualitative measure of orientation can be obtained.





From daemon Mon Mar 20 07:46:07 2000



From: IAN HALLETT :      ihallett-at-hort.cri.nz
Date: Mon, 20 Mar 2000 16:45:33 +1300
Subject: Cryo-SEM, mounting frozen powder

Contents Retrieved from Microscopy Listserver Archives
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Dear All

I've been asked whether it is possible to look at a frozen powder
using Cryo-SEM. The powder will be frozen and stored at -80 C and
must be mounted whilst frozen (it may be possible to raise the
temperature to -20 C) any ideas of an adhesive I could use at these
low temperatures??

Thanks

Ian


Ian Hallett
HortResearch
Mt Albert Research Centre
Private Bag 92 169
Auckland, New Zealand
Fax 64-9-815 4201
Telephone 64-9-815 4200
EMail ihallett-at-hort.cri.nz



From daemon Mon Mar 20 07:46:14 2000



From: Robin Cross :      r.cross-at-ru.ac.za
Date: Mon, 20 Mar 2000 08:56:24 +0200
Subject: SEM maintenance & historical info

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Earl

} As far as your analysis of the SEM chambers & polepieces, historically
} the SEM industry has seen the large chamber sizes and conical polepieces
} you decribe for at least last twenty years.

For those, like me, who were interested in your recollections about
SEM chamber and lens design, and who may be considering
attending ICEM-15 in South Africa in 2002, make a note that there
will be an exhibition at the congress retracing the history of
electron microscopy in South Africa. This will include a variety of
old EM equipment, including some of the SEM's to which you
referred.

Regards

Rob



Robin H Cross
Chairman : 15th International Congress on Electron Microscopy (ICEM-15)
tel: +27 46 603 8168/9 - fax: +27 46 622 4377
email: r.cross-at-ru.ac.za
http://www.ru.ac.za/emu/icem-15.htm

** remember that ICEM-15 in 2002 is in Durban, South Africa **



From daemon Mon Mar 20 07:46:20 2000



From: Robin Cross :      r.cross-at-ru.ac.za
Date: Mon, 20 Mar 2000 15:06:28 +0200
Subject: Re: Cryo-SEM, mounting frozen powder

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ian,
I've used Duro-Tak¨ 80-1061 to hold particles during analysis at liquid
nitrogen temperatures . For more information see:
http://www.fbi.gov/programs/lab/fsc/backissu/july1999/ward.htm

Dennis
________________________________________________________________________
Dennis C . Ward voice: 202-324-2982
FBI Laboratory fax: 202-324-4018
Microanalysis Laboratory email:
DCWard-at-concentric.net


----- Original Message -----
} From: "IAN HALLETT" {ihallett-at-hort.cri.nz}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Sunday, March 19, 2000 7:45 PM


Hello Ian

} I've been asked whether it is possible to look at a frozen powder
} using Cryo-SEM.

That shouldn't pose any problems. Choice of adhesive would
depend on the characteristics of the powder.

Rob



Robin H Cross
Director : EM Unit, Rhodes University, Grahamstown, South Africa
R.Cross-at-ru.ac.za
tel: +27 46 603 8168 - fax: +27 46 622 4377
http://www.ru.ac.za/emu/index.htm

** remember that ICEM-15 in 2002 is in Durban, South Africa **



From daemon Mon Mar 20 07:46:27 2000



From: Carstensen, Jesper Vejl¿
Date: Mon, 20 Mar 2000 13:35:51 +0100
Subject: THANKS: Carbides in tool steels

Contents Retrieved from Microscopy Listserver Archives
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Dear all,

I would like to thank those who took the time to respond to my e-mail. I
received a lot of valuable information and advice on different techniques to
be used (and NOT to be used) when studying carbides in tool steels. I
appreciate your help.

Kind regards, Jesper

----------------------------------------------------
Jesper Vejloe Carstensen
Research Scientist, M.Sc., Ph.D.
Materials Research Department
Risoe National Laboratory
P.O. Box 49
DK-4000 Roskilde, Denmark
Phone: +45 4677 5776
Fax: +45 4677 5758
E-mail: jesper.v.carstensen-at-risoe.dk
Web: http://www.risoe.dk/AFM/Personal/Jeca/jeca.htm
----------------------------------------------------






From daemon Mon Mar 20 07:57:28 2000



From: Stefan.Geimer :      stefan.geimer-at-uni-koeln.de
Date: Mon, 20 Mar 2000 14:59:40 +0000
Subject: 35 Degree Angle Diamond Knive

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We are planning to buy a new diamond knive for sectioning biological
samples. My question is if anybody has experience with using 35 degree
angle diamond knives and if they have any disantvantages compared to 45
degree angle diamond knives. We are mainly sectioning unicellular algae
embedded in LR-gold and hope to reduce compression problems by using a
35 degree angle knive. Our problem is that, especially algae with thick
cell walls, tend to fall out of the sections. This problem seems to be
caused by compressions, because using a brand-new 45 degree angle
diamond knive I had much less algae falling out of the sections. Because
we can effort only one new knive we have to use it as an all-round
knive. Is a 35 degree knive suitable to replace a 45 degree knive or is
it much more fragile and/or less durable?


Thanks in advance for your time and help.

Sincerely,

Stefan Geimer



***********************
Stefan Geimer
University of Cologne
Botanical Institute
Gyrhofstr. 15
D-50931 Cologne
Germany
phone: +49-(0)221-470-3795
fax: +49-(0)221-470-5181
************************




From daemon Mon Mar 20 13:01:21 2000



From: Marisa Ahmad :      mahmad-at-semiconductor.com
Date: Mon, 20 Mar 2000 10:50:45 -0500
Subject: Liquidation of assets

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Apparently there is more equipment lying around that needs a good home. If
anyone is interested, please respond directly to Mark Chambers via
mark-at-semiconductor.com or at (613) 599-6500 ext 4269.

Marisa

} -----Original Message-----
} From: Mark Chambers
} Sent: Friday, March 17, 2000 4:18 PM
} To: Marisa Ahmad
} Subject: RE: Liquidation of assets
}
} Hi Marisa,
}
} Is the listserver you used for the PGT EDS announcement a suitable venue
} for the plasma asher and the Mosaid tester?
}
} The barrel etcher is a Plasmaline model 411 and whoever wants it will need
} to get a vacuum pump for it. The etcher was working but it was not an
} efficient way to decap packages.
}
} The Mosaid tester is a model MS2300 and that's basically all I know about
} it.
}
} Thanks
}
Mark



From daemon Mon Mar 20 13:01:22 2000



From: Soumitra Ghoshroy :      ghoshroy-at-nmsu.edu
Date: Mon, 20 Mar 2000 09:08:21 -0700
Subject: Re: accu-edge blades

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


You can buy them from Electron Microscopy Sciences. 1-800-523-5874


Soumitra




*****************************************************************
Soumitra Ghoshroy Ph.D.
Electron Microscopy Lab
Box 3EML
New Mexico State University
Las Cruces, NM 88003
Tel: 505-646-3600
Fax: 505-646-5665
e-mail: ghoshroy-at-nmsu.edu
http://confocal.nmsu.edu/eml




From daemon Mon Mar 20 13:01:23 2000



From: Bob Phillips :      microservis-at-dial.pipex.com
Date: Mon, 20 Mar 2000 16:34:38 -0000
Subject: JEOL 100CX looking for new home

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Everybody,

I have been asked to find a new home for a fully working JEOL 100CX tem
which is currently in use in the south of England. It has the ASID stem
attachment, and it could also be available with a Link AN10 analysis system.

Probably the only cost will be the dismantling & removal charges. If anyone
is interested, please reply to me directly. My only commercial interest is
that I might be involved with the dismantling etc.

Bob Phillips
MicroServiS
bob.phillips-at-microservis.co.uk




From daemon Mon Mar 20 13:01:25 2000



From: Dr G. R. Coulton [bs_mp] :      g.coulton-at-ic.ac.uk
Date: Mon, 20 Mar 2000 17:24:44 +0000
Subject: Microscopy/Cell Biology & Imaging at ICHC 2000

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Colleague,

ICHC 2000

Please check out the web-site for ICHC 2000 (11th International Congress
for Histochemistry and Cytochemistry) 3-8th Sept. York, UK.

We have put together a fabulous speaker list covering a range of the most
important areas of development and application of cutting edge techniques
in Cell Biology and Imaging today. Lead speakers include Roger Tsien,
Stefan Hell, Alan Fine, Alan Bode, Hans Tanke, Jennifer
Lippincott-Schwartz, Angus Lamond, Jim Smith, Richard Haugland, Paul
Monaghan, Mike Ormerod, Simon Gilroy Nick White etc. etc. etc.

www.med.ic.ac.uk/external/ichc_2000

ABSTRACT DEADLINE 15TH MAY 2000.

Best wishes

Gary Coulton
Dr. Gary Coulton
Molecular Pathology
Division of Biomedical Sciences
Imperial College School of Medicine
The Sir Alexander Fleming Building
South Kensington
London SW7 2AZ

tel 0044 (0)171 594 3190
fax 0044 (0)171 594 3022

PLEASE NOTE AS FROM APRIL 1ST 2000 THE FOLLOWING NUMBERS WILL REPLACE THOSE
ABOVE AND SHOULD BE USED.

TEL. ++44 020 7594 3190
FAX. ++44 020 7594 3022
e-mail g.coulton-at-ic.ac.uk


-------------------------------------
Announcing the 11th International Congress of Histochemistry and
Cytochemistry (ICHC 2000)

"Understanding Biocomplexity: The Post-Genome Challenge"

September 3-8, 2000, York, United Kingdom

ICHC 2000 will comprise 27 symposia addressing the latest developments and
applications of histochemistry and cytochemistry in the life sciences
including medicine.

Many leading experts to speak

On-line registration now open!!!!!!!!!!

For further details of the meeting and how to pre-register please visit our
web-site at http://www.med.ic.ac.uk/external/ichc_2000

Hope to see you there.



From daemon Mon Mar 20 13:01:26 2000



From: Doug Yates :      dmyates-at-lrsm.upenn.edu
Date: Mon, 20 Mar 2000 12:52:40 -0500
Subject: Technical Staff Position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Technical Staff Position:

The Department of Materials Science and Engineering at the University of
Pennsylvania is seeking an engineer or scientist for a technical staff
position in its advanced characterization facility. This facility contains
a number of state-of-the-art microscopes and scattering facilities as well
as ancillary detectors, specimen preparation equipment, and data processing
and computational hardware and software. The facility serves Penn research
programs, as well as academic, industry and government users from the
Delaware Valley region and beyond. The University of Pennsylvania is
located in Philadelphia, one of the nation's most vibrant cities. The
university, a member of the Ivy League, was founded by Ben Franklin and is
the fourth oldest and first secular university in the US. The Laboratory
for Research on the Structure of Matter was constructed to house one of the
original three Materials Research Laboratories (MRLs) in the US. The
university has a continuing tradition of leading materials research and has
housed the MRL (now MRSEC) continuously for 40 years.

Under the direction of the facility manager, the successful candidate will
be responsible for conducting experiments with users on facility equipment,
training new users and maintenance of facility equipment not covered under
service contract. The successful candidate must have the communication
skills and self-confidence to interact with technical users with a wide
range of expertise and background. Job performance will be assessed based
on the success in experimental interactions with users, the operational
state of equipment for which the staff member is responsible, progress in
the acquisition of new skills, and facility appearance.

A Bachelors degree in physical science or engineering is required, although
an advanced degree is preferred. The successful candidate must have
experience with electronics, ultra-high vacuum technology and computer
interfacing of equipment. Experience in the operation and use of electron
microscopes and ion beam lines and associated end-stations is
desirable. Experience in the use of Auger electron spectroscopy or scanned
probe imaging is also desirable.

The text of the official job listing from the University of Pennsylvania
web site follows. Please refer to the Penn Human Resources web site for the
official hiring policy of the university at www.hr.upenn.edu. For
information about the specific position, please contact the facility
manager, Dr. Douglas Yates, at dmyates-at-lrsm.upenn.edu.


Text of web site listing:

Reference Number: 00034808DL
Job Title: RESEARCH COORDINATOR SR
School/Center: ENGINEERING & APPLIED SCIENCE
Department: MATERIALS CHARACTERIZATION
Date Posted: 3/9/00

Salary Grade: 026
Employee Type: Exempt, Monthly Paid
Position Length: Ongoing

Duties: Train or coordinate training of facility users; assist users
performing experiments; compose monthly MCF users billing summary; organize
purchasing of consumable laboratory materials; maintain or coordinate
maintenance of equipment; maintain working environment & appearance of
laboratory.

Qualifications: BA/BS in Physical Science or Engineering required, advanced
degree preferred; 3 to 5 years experience with electronics, ultra-high
vacuum technology & computer interfacing of equipment & in operation & use
of electron microscopes, ion beam lines and/or scanned probe imaging.


*******************************************************
Douglas M. Yates, Ph.D.
Manager, Materials Characterization Facility

(215) 573-6123
dmyates-at-lrsm.upenn.edu

University of Pennsylvania
Department of Materials Science & Engineering
3231 Walnut Street
Philadelphia, PA 19104-6272
*******************************************************



From daemon Mon Mar 20 13:11:50 2000



From: HILDEGARD CROWLEY :      hcrowley-at-du.edu
Date: Mon, 20 Mar 2000 12:00:52 -0700 (MST)
Subject: Re: 35 Degree Angle Diamond Knive

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

One of your best sources of information is going to be Diatome USA. They
have an actual lab set up to test cutting problems of a large variety.
They also have access to the information of the company who makes these
knives.

Please consider that your problem may not be with the knife or the angle
at which the knife is sharpened. Most "breaking out" problems are related
to the production of the block, especially 1) the infiltration 2) the
match between the specimen and the formulation used. Contact Diatome, at
EMS, and ask to speak with Stacy Kirsch, who is an expert with these
problems. (I have no business interest in Diatome - I have just received
the most excellent advice from them over the years)

Hildy Crowley
Sr. Electron Microscopist
University of Denver
Denver, CO

P.S. Should processing be the basic problem, please contact me. I may be
able to help you.



From daemon Mon Mar 20 13:11:50 2000



From: Michael Bode :      mb-at-Soft-Imaging.com
Date: Mon, 20 Mar 2000 11:38:35 -0700
Subject: FW: Need help on PEELS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Sir,

My specialists tell me, that Boron shows a plasma loss at 22.7 eV and a
K-edge at 188 eV. They also tell me, that it is hard to see B in samples
as it usually disappears rapidly. To see B, they suggest focusing on a
different sample area and then only expose the area in question for the
PEELS acquisition.

In case you are using our software on a LEO microscope for the
acquisition, you should select the MDF option (Micro Dose Focusing).

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Exchange Administrator
Sent: Saturday, March 11, 2000 12:35 PM
To: Michael Bode



Need help on PEELS
}
} To all,
}
} I am working on grain boundary chemistry measurement
} with Digipeels. Try to find out the segregation
} behavior of boron, which is about 0.5at% averagely.
} However, I cannot find any edge for boron in the
} spectrum. As I don't know what's the optimum setting
} for PEELS to check the grain boundary segregation,
if
} you have any idea on this, please let me know.
}
} Thanks,
} Lung
__________________________________________________
Do You Yahoo!?
Talk to your friends online with Yahoo! Messenger.
http://im.yahoo.com




From daemon Mon Mar 20 17:55:28 2000



From: Rick Harris :      raharris-at-ucdavis.edu
Date: Mon, 20 Mar 2000 13:50:34 -0800
Subject: JEOL 100SX going to salvage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We bought a JEOL 100SX for about $130,000 in 1987. The scope was for
student use in an undergraduate course. It was used for 4 to 8 weeks per
year for 7 years. It was used very little. About 1994, the water chiller
failed and cooked the objective mini-lens and some other electronics. It
was one year off the service contract. JEOL could not guarantee that they
could fix it for less than $11,000 so it was moth balled. It was
functional at 8000X and higher. We have to get rid of this in a real
hurry. It someone wants some or all of this machine please contact me
ASAP. Within about a week it will be in the dump. I recall that at the
time it failed there was a user at Stanford (I think) who had the same
model. If anyone knows who that was, please have him contact me.




Rick A. Harris, Director
Microscopy and Imaging Facility
Section of Molecular and Cellular Biology
1241 Life Sciences Addition
University of California
Davis, CA
530 752 2914
530 754 7536 fax
http://katie.ucdavis.edu
raharris-at-ucdavis.edu



From daemon Mon Mar 20 17:55:29 2000



From: Everett Ramer :      Everett.Ramer-at-netl.doe.gov
Date: Mon, 20 Mar 2000 17:22:28 -0500
Subject: EDS in Variable Pressure SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




It seems that nowadays every SEM vendor is offering variable pressure models, which are conventional SEMs with a plumbing modification that allows the sample to be at pressures of up to about 4 torr (500 Pa) while the electron column operates at the conventional high vacuum. I am very interested in buying a variable pressure SEM with an EDS, but was recently warned that EDS has very poor spatial resolution in the variable pressure mode because of the large beam spread due to electrons scattering off the gas molecules in the sample chamber. Is this really a significant issue? Do any of you have experience using EDS with variable pressure SEMs?
Thanks,



From daemon Mon Mar 20 17:55:34 2000



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Mon, 20 Mar 2000 17:09:07 -0600
Subject: Re: EDS in Variable Pressure SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In short, it is an issue, but not intractable. For careful quantitative
work we reduce the vacuum as much as the sample permits. We can usually get
by with about 40 Pa of helium atmosphere on concrete samples. Even at 40 Pa
we can pick up percent levels of elements away from the beam. For example,
a Co chip in a standard mount can easily show 1% Fe from the stainless
steel carrier. For qualitative work, that is not much of a problem, but if
you are looking for trends in that element (Fe in Co), it would be
impossible in low vacuum mode.

BTW, Helium greatly reduces the scattering compared to air at the same
pressure.

I would encourage you to go ahead and get the VP SEM, but work through a
few exercises with it to get a feel for its limits.

At 05:22 PM 3/20/2000 -0500, you wrote:
} It seems that nowadays every SEM vendor is offering variable pressure
} models, which are conventional SEMs with a plumbing modification that
} allows the sample to be at pressures of up to about 4 torr (500 Pa) while
} the electron column operates at the conventional high vacuum. I am very
} interested in buying a variable pressure SEM with an EDS, but was recently
} warned that EDS has very poor spatial resolution in the variable pressure
} mode because of the large beam spread due to electrons scattering off the
} gas molecules in the sample chamber. Is this really a significant issue?
} Do any of you have experience using EDS with variable pressure SEMs?
} Thanks,

----------------------
Warren E. Straszheim
Materials Analysis and Research Lab
Iowa State University
23 Town Engineering
Ames IA, 50011-3232

Ph: 515-294-8187
FAX: 515-294-4563

E-Mail: wesaia-at-iastate.edu
Web: www.marl.iastate.edu

Scanning electron microscopy, x-ray analysis, and image analysis of materials
Computer applications and networking



From daemon Mon Mar 20 18:28:24 2000



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Mon, 20 Mar 2000 16:01:36 -0800
Subject: Re: 35 Degree Angle Diamond Knive

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


It is a good point to contact DIATOME. It reminds to me, also, that they
(DIATOME) may cut your samples on site and sent back the grids (if I
understand them correctly) for free. So, you may try 45 and 35o knife and
compare the results before you make final decision. As for 35o. 45o is
more universal and durable. If you rich enough only for one diamond knife,
it should be 45o, I believe. 35o supposed to be a "second knife" for
"special occasions". I have no financial interest in DIATOME, but happy
user of DIATOME knife

Sergey

} Date: Mon, 20 Mar 2000 12:00:52 -0700 (MST)
} From: HILDEGARD CROWLEY {hcrowley-at-du.edu}
} Subject: Re: 35 Degree Angle Diamond Knive
} X-Sender: hcrowley-at-odin.cair.du.edu
} To: "Stefan.Geimer" {stefan.geimer-at-uni-koeln.de}
} Cc: Microscopy-at-sparc5.microscopy.com
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu
http://www.bol.ucla.edu/~sryazant




From daemon Mon Mar 20 18:58:31 2000



From: Malis, Tom :      malis-at-nrcan.gc.ca
Date: Mon, 20 Mar 2000 19:49:35 -0500
Subject: RE: 35 Degree Angle Diamond Knive

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Diatome, for one, can supply you with clear ev idence for significantly
reduced compression with a 35 degree knife over a 45 degree knife. No
surprise there, since compression should be reduced with knife angle in
materials which compress significantly. We are a 'materials science' lab,
where we section all manner of metals and alloys, composites, powders,
fibres, coatings, thin films, etc, etc. For us reduced compression is not a
big deal, but durability obviously is. I can report that, for the last 5
years or so since their acquisition, our two Diatome 35 degree knives are
used about 90% of the time, with no catastrophic failures or large increase
in sharpening frequency.

I agree with Hildegard Crowley that breaking out is far more likely to be
due to embedding/bonding problems. another possibility is simply a dull
edge. Your increased success with a fresh (presumably demo) knife could
indicate the latter. If the edge is quite rounded from use, the effective
angle at the point of sectioning will shoot up incredibly, which will then
increase the compressive forces that expose any embedding/bonding
deficiencies.

That said, if you can only afford one knife, look in the mirror and ask
yourself how careful are the people who might use the knife. A simple
consideration of the forces on a knife edge during sectioning will tell you
that a lesser angle should be slightly more susceptible to side forces.
However, just the thought of a reduced angle might make an inexperienced
operator sufficiently nervous to increase that risk greatly!

Tom Malis
Group Leader - Characterization
Materials Technology Laboratory
Natural Resources Canada (Govt. of Canada)
568 Booth St., Ottawa, Canada
ph. 613-992-2310
FAX 613-992-8735
email: malis-at-nrcan.gc.ca


} ----------
} From: Stefan.Geimer
} Sent: Monday, March 20, 2000 9:59 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: 35 Degree Angle Diamond Knive
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} We are planning to buy a new diamond knive for sectioning biological
} samples. My question is if anybody has experience with using 35 degree
} angle diamond knives and if they have any disantvantages compared to 45
} degree angle diamond knives. We are mainly sectioning unicellular algae
} embedded in LR-gold and hope to reduce compression problems by using a
} 35 degree angle knive. Our problem is that, especially algae with thick
} cell walls, tend to fall out of the sections. This problem seems to be
} caused by compressions, because using a brand-new 45 degree angle
} diamond knive I had much less algae falling out of the sections. Because
} we can effort only one new knive we have to use it as an all-round
} knive. Is a 35 degree knive suitable to replace a 45 degree knive or is
} it much more fragile and/or less durable?
}
}
} Thanks in advance for your time and help.
}
} Sincerely,
}
} Stefan Geimer
}
}
}
} ***********************
} Stefan Geimer
} University of Cologne
} Botanical Institute
} Gyrhofstr. 15
} D-50931 Cologne
} Germany
} phone: +49-(0)221-470-3795
} fax: +49-(0)221-470-5181
} ************************
}
}
}
}



From daemon Mon Mar 20 19:28:22 2000



From: maxwell-at-lec.med.utoronto.ca () (by way of Nestor J. Zaluzec)
Date: Mon, 20 Mar 2000 19:20:58 -0600
Subject: bacteriophage question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Colleagues,

Can anyone help Karen Maxwell?, reply to her as well as the list
as she is not a subscriber.

Nestor
Your Friendly Neighborhood SysOp



Below is the result of your feedback form. It was submitted by
(maxwell-at-lec.med.utoronto.ca) on Wednesday, March 15, 2000 at 17:03:53
---------------------------------------------------------------------------

Email: maxwell-at-lec.med.utoronto.ca
Name: Karen Maxwell

School: University of Toronto

State: Ontario

Question: I have been looking at a paper from 1984 (Kochan et al) where
they used EM to study the preconnector from bacteriophage lambda. They
discovered that the axial hole in the ring shaped structure was 2.2-2.5 nm
in diameter. They used negative staining with ammonium molybdate, uranyl
acetate, and uranyl formate. In more recent studies (Valpuesta et al,
1999), the connector of phi29 was examined using a three-dimensional
cryo-reconstruction, and the axial hole was found to be 3.3 nm. These are
two different phage, but my question is, could the old techniques used in
the case of the bacteriophage lambda preconnector (negative staining) have
under-estimated the diameter of the axial hole? And if so, do you have an
idea of the percent error that may be inherent in the technique? Is the
cryo-reconstuction likely to give a value that is more accurate? Could you
recommend any references that would address these questions?

Thanks in advance for your help.

Karen Maxwell

---------------------------------------------------------------------------




From daemon Mon Mar 20 21:28:19 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 20 Mar 2000 19:16:10 -0600
Subject: Re: EDS in Variable Pressure SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 04:22 PM 3/20/00 , you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

I have not had an opportunity to try these systems but from what I can
glean, they are not totally similar to conventional SEMs. In particular,
the VP SEMs do not use the E-T detector, but rather a gas discharge
detector. There are some good examples of these images around but
I do wonder what the actual difference is. Perhaps owners can tell us.
Or warn us???

gary g.



From daemon Tue Mar 21 07:32:46 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 20 Mar 2000 19:16:10 -0600
Subject: Re: EDS in Variable Pressure SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 04:22 PM 3/20/00 , you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

I have not had an opportunity to try these systems but from what I can
glean, they are not totally similar to conventional SEMs. In particular,
the VP SEMs do not use the E-T detector, but rather a gas discharge
detector. There are some good examples of these images around but
I do wonder what the actual difference is. Perhaps owners can tell us.
Or warn us???

gary g.




From daemon Tue Mar 21 07:32:48 2000



From: Joergen Bilde-Soerensen 5802 :      j.bilde-at-risoe.dk
Date: Tue, 21 Mar 2000 10:03:54 +0100
Subject: Re: EDS in Variable Pressure SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Everett Ramer wrote:
} It seems that nowadays every SEM vendor is offering variable pressure
} models, which are conventional SEMs with a plumbing
} modification that allows the sample to be at pressures of up to
} about 4 torr (500 Pa) while the electron column operates at the
} conventional high vacuum. I am very interested in buying a
} variable pressure SEM with an EDS, but was recently warned that
} EDS has very poor spatial resolution in the variable pressure
} mode because of the large beam spread due to electrons
} scattering off the gas molecules in the sample chamber. Is this
} really a significant issue? Do any of you have experience using
} EDS with variable pressure SEMs?
} Thanks,

The fraction of primary electrons that are scattered by the gas in
the specimen chamber is approximately given by:

1-I/Io = 1 - exp(-psL/kT)

where p is the pressure
s the total scattering cross section for scattering of x keV
electrons on the gas used
L the distance between the last pressure limiting aperture and
the sample
k the Boltzmann constant
and T the absolute temperature.

You can therefore reduce the contribution of X-rays excited by
scattered primary electrons by reducing p (lowest possible
pressure), s (use gas with low scattering cross section and the
highest possible acceleration voltage balanced against
overvoltage considerations) and L.

As Warren Straszheim wrote this will overcome the beam skirt
problem to a large degree. In the situations where this is not
sufficient, you can use an extrapolation method that has been
developed here at Risoe National Laboratory. In short, you
perform the analysis at various different pressures and use the
above equation to extrapolate to the result you would get under
zero pressure. A more detailed description can be found at the
web-address

{ HYPERLINK http://www.risoe.dk/afm/news1new.htm }http://www.risoe.dk/afm/news1new.htm

Best regards,
Joergen Bilde-Soerensen


*****************************
J. B. Bilde-Soerensen
Senior Research Scientist, Ph. D.
Materials Research Department
Risoe National Laboratory
DK-4000 Roskilde
Denmark

e-mail: j.bilde-at-risoe.dk
phone: +45 46 77 58 02 (direct)
phone: +45 46 77 46 77 (switchboard)
fax: +45 46 77 57 58
website: http://www.risoe.dk/afm/Personal/jqbi/jqbi.htm



From daemon Tue Mar 21 07:32:53 2000



From: GuessWho :      olivier.balmes-at-oorg2.lth.se
Date: Tue, 21 Mar 2000 11:26:23 +0100
Subject: EFTEM/GIF on particles

Contents Retrieved from Microscopy Listserver Archives
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Dear all,

I was wondering if anybody was using a GIF filter. We have one mounted
on a 120 kV TEM and are trying to image small particles (5-10 nm) and
doing some energy filtering to image different elements in the particles
(for example gold, silicon, copper, cerium...) . This is not really what
I would call trivial work and we are still working out the set up
(window size and positionning for example) in a rather empiric way. The
main problem is often to get a decent signal in the interesting energy
region and still keep the windows fairly narrow.
I would add that we often have to work at low dose because of the beam
sensitivity of our samples, but I guess our GIF setups can be improved.
Any suggestion ?

Any general comment on the GIF warmly welcome !

Thanks

Olivier



From daemon Tue Mar 21 07:32:55 2000



From: A.P. Alves de Matos :      apmatos-at-ip.pt
Date: Tue, 21 Mar 2000 11:29:14 -0000
Subject: Hoechst 33258 counter stain

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Dear Collegues

I am staining virus infected cell DNA (in cell culture) with Hoechst 33258
fluorescent stain. I need to make correlation of the fluorescent spots with
cellular structure.

Do you know of any counter-stain that can be used without destroying the
fluorescent Hoechst staining?

Thanks
Dr. A.P. Alves de Matos
biologist
apmatos-at-ip.pt



From daemon Tue Mar 21 07:32:56 2000



From: Florian Dalla Torre :      Florian.dallatorre-at-psi.ch
Date: Tue, 21 Mar 2000 13:30:29 +0100
Subject: Electrolytical thinning of nanocrystalline Nickel for TEM

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Hi all,

Does anybody now the solution, temperature and voltage for thinning
electrolytically Nickel (nanocrystalline) for TEM samples?

--
Florian Dalla Torre
Paul Scherrer Institut
CH / 5232 Villigen PSI
Switzerland
email: Florian.DallaTorre-at-psi.ch
phone ++41/56/310 35 66
fax ++41/56/310 31 31




From daemon Tue Mar 21 07:50:41 2000



From: Purdy, Sam :      SPurdy-at-nationalsteel.com
Date: Tue, 21 Mar 2000 07:37:17 -0600
Subject: RE: accu-edge blades

Contents Retrieved from Microscopy Listserver Archives
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Diana:
TAAB Laboratories Equipment, Ltd,
3 Minerva Court House,Calleva Park
Aldermaston, Berks,RG7 8NA, UK

They list single edge razor blades, with and without a bac k in
stainless and carbon steels. They also list double edge razor blades, 0.004
in. thick, in stainless steel.

Best regards,

Sam Purdy
} ----------
} From: Jeff & Wanda Gray
} Sent: March 2000 10:12 PM
} To: Diana Papoulias; microscopy-at-sparc5.microscopy.com
} Subject: RE: accu-edge blades
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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} -----------------------------------------------------------------------.
}
}
} Diana,
} These blades are available from Allegiance Scientific (used to be Baxter,
} used to be Scientific Products, used to be....). As far as I know, this is
} the only place that carries this brand.
} I have no financial interest in Allegiance, just passing along a fact.
} Wanda Shotsberger
} (HT ASCP)
}
} -----Original Message-----
} } From: Diana Papoulias [mailto:Diana_Papoulias-at-usgs.gov]
} Sent: Thursday, March 16, 2000 2:53 PM
} To: microscopy-at-sparc5.microscopy.com
} Subject: accu-edge blades
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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} -----------------------------------------------------------------------.
}
}
} Could someone please tell me where I can purchase accu-edge microtome
} blades?
}
} Thank you.
}
} Diana Papoulias
} USGS
} 4200 New Haven Rd
} Columbia, MO 65201
}
} T:573 876 1902
} F:573 876 1876
} E:Diana_Papoulias-at-usgs.gov
}
}
}




From daemon Tue Mar 21 07:57:26 2000



From: Hans Dijkstra :      hans.dijkstra-at-edax.nl
Date: Tue, 21 Mar 2000 14:53:31 +0100
Subject: RE: EDS in Variable Pressure SEM

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Dear Everett,

The extrapolation method (aka Variable Pressure Method) described by Dr.
Bilde-Sorenson has been implemented by EDAX in a software feature called
ViP-Quant. With this method the EDS analysis under Bad-Vacuum conditions can
be done with nearly the same accuracy as under High-Vacuum conditions.
Although this method was developed with the ESEM microscope in mind, it of
course can be applied to all Bad-Vacuum scanning electron microscopes.

Please contact your local EDAX representative for more information and a
copy of the new ViP-Quant brochure, or request one through www.edax.com.

With best regards,

Hans Dijkstra

-------------------------------------------------------------
EDAX Europe www.edax.com
Ringbaan Noord 103 Tel.: +31-13-5364000
P.O. Box 4144 Fax.: +31-13-5356279
5004 JC Tilburg E-mail: hans.dijkstra-at-edax.nl
the Netherlands
-------------------------------------------------------------

} -----Original Message-----
} From: Joergen Bilde-Soerensen 5802 [mailto:j.bilde-at-risoe.dk]
} Sent: Tuesday, March 21, 2000 10:04 AM
} To: Everett Ramer
} Cc: Microscopy-at-sparc5.microscopy.com
} Subject: Re: EDS in Variable Pressure SEM
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} Everett Ramer wrote:
} } It seems that nowadays every SEM vendor is offering variable pressure
} } models, which are conventional SEMs with a plumbing
} } modification that allows the sample to be at pressures of up to
} } about 4 torr (500 Pa) while the electron column operates at the
} } conventional high vacuum. I am very interested in buying a
} } variable pressure SEM with an EDS, but was recently warned that
} } EDS has very poor spatial resolution in the variable pressure
} } mode because of the large beam spread due to electrons
} } scattering off the gas molecules in the sample chamber. Is this
} } really a significant issue? Do any of you have experience using
} } EDS with variable pressure SEMs?
} } Thanks,
}
} The fraction of primary electrons that are scattered by the gas in
} the specimen chamber is approximately given by:
}
} 1-I/Io = 1 - exp(-psL/kT)
}
} where p is the pressure
} s the total scattering cross section for scattering of x keV
} electrons on the gas used
} L the distance between the last pressure limiting aperture and
} the sample
} k the Boltzmann constant
} and T the absolute temperature.
}
} You can therefore reduce the contribution of X-rays excited by
} scattered primary electrons by reducing p (lowest possible
} pressure), s (use gas with low scattering cross section and the
} highest possible acceleration voltage balanced against
} overvoltage considerations) and L.
}
} As Warren Straszheim wrote this will overcome the beam skirt
} problem to a large degree. In the situations where this is not
} sufficient, you can use an extrapolation method that has been
} developed here at Risoe National Laboratory. In short, you
} perform the analysis at various different pressures and use the
} above equation to extrapolate to the result you would get under
} zero pressure. A more detailed description can be found at the
} web-address
}
} { HYPERLINK http://www.risoe.dk/afm/news1new.htm
}http://www.risoe.dk/afm/news1new.htm

Best regards,
Joergen Bilde-Soerensen


*****************************
J. B. Bilde-Soerensen
Senior Research Scientist, Ph. D.
Materials Research Department
Risoe National Laboratory
DK-4000 Roskilde
Denmark

e-mail: j.bilde-at-risoe.dk
phone: +45 46 77 58 02 (direct)
phone: +45 46 77 46 77 (switchboard)
fax: +45 46 77 57 58
website: http://www.risoe.dk/afm/Personal/jqbi/jqbi.htm




From daemon Tue Mar 21 07:57:26 2000



From: Mark Sanders :      msanders-at-biosci.cbs.umn.edu
Date: Tue, 21 Mar 2000 07:54:37 +0000
Subject: Opening Leica TCS SP files

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Does anyone have a macro to open up individual channels of Leica TCS
confocal files for NIH/Scion Image?
Thanks!
ED



From daemon Tue Mar 21 18:21:36 2000



From: Centro de Im‡genes y Microscop’a
Date: Tue, 21 Mar 2000 08:02:51 -0600
Subject: fixating and including frozen pieces

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Dear Colleagues,
I want to fix insects at particular moments of muscle activity. I
plan to
freeze the bugs in liquid nitrogen and section their heads, after including
them in hard Durcupan. I have no experience in fixating and including
frozen pieces. Note that hard resin should be used, provided the hardness
of the insect cuticle. Does anybody know a simple method for the
transference from liquid nitrogen to plastic?
Thank your very much in advance,
Claudio
Dr. Claudio R.Lazzari
lazzari-at-bg.fcen.uba.ar

------------------------------------------
Laboratorio de Fisiolog’a de Insectos
Dpto. Cs. Biol—gicas, Univ. Buenos Aires
Ciudad Universitaria, 1428 Buenos Aires
Argentina
Tel.(54 11) 4576 3300, ext. 332, FAX (54 11) 4576 3384




Gabriel Adriano Rosa
Centro de Imagenes y Microscopia
Departamento de Ciencias Biologicas
Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires
Ciudad Universitaria, 4 piso, Pab. II, CP 1428, Buenos Aires, ARGENTINA
TE -(54-11)-4576-3349 e-mail cimic-at-bg.fcen.uba.ar
FAX (54-11)-4576-3384




From daemon Tue Mar 21 18:21:37 2000



From: Tamara Howard :      howard-at-cshl.org
Date: Tue, 21 Mar 2000 09:36:07 -0500 (EST)
Subject: Re: DNA stain summary

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I don't think 7-AAD will work - it is used as a live/dead stain, and I
know that it works much better on detergent-permeabilized cells than on
merely PFA-fixed ones.

If you are contemplating microinjection (ugh!), maybe try the fluorescent
dextrans instead of UTPs...they won't mess up your nucleic acids. We use
them as injection markers and they don't seem to harm the cells.

Tamara Howard
CSHL

On Tue, 21 Mar 2000, Donald O'Malley wrote:

} Hi Folks,
}
} Thanks for the flurry of info about DNA/RNA staining. I've
summarized some of this info below in case anyone else has needs related
to ours.
} What I neglected to mention is that we are trying to count nuclei
inside of living mouse embryos. That's why we're searching for a
non-invasive, membrane crossing, nuclear-selective dye.
}
} Summary of recent listserv messages:
}
}
} Possible Dyes for live TISSUE, visible wavelength,
} selective NUCLEAR staining:
}
} 7 aminoactinomycin. But does it cross membranes?
} microinjected Alexa-UTPs



From daemon Tue Mar 21 18:21:38 2000



From: Barbara Foster :      mme-at-map.com
Date: Tue, 21 Mar 2000 09:50:23 -0500
Subject: Re: EDS in Variable Pressure SEM

Contents Retrieved from Microscopy Listserver Archives
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Dear Everett,

I just got back from PITTCON. When I was visiting the EDAX booth they
showed me a new program through which they have resolved this problem. I
would suggest at least inquiring.

Best regards,
Barbara Foster
Consortium President
Microscopy/Microscopy Education ...Educating microscopists for greater
productivity.

125 Paridon Street Suite 102 Springfield, MA 01118
PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com
Visit our web site {http://www.MME-Microscopy.com/education}
******************************************************
MME is America's first national consortium providing
customized on-site workshops in all areas of
microscopy, sample preparation, and image analysis.


At 05:22 PM 3/20/00 -0500, Everett Ramer wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Tue Mar 21 18:22:02 2000



From: Mary Gail Engle :      mgengle-at-pop.uky.edu
Date: Tue, 21 Mar 2000 17:12:39 -0500
Subject: Hoechst 33258

Contents Retrieved from Microscopy Listserver Archives
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Dr. Alves de Matos,
Regarding your question about counter staining for cellular structure--have
you considered scanning your Hoechst image and overlaying a Nomarski
image? If this doesn't yield enough detail there are fluorescent dyes
specifically for organelles such as mitochondria and golgi. Molecular
Probes sells them.

Mary Gail Engle
Electron Microscopy & Imaging Facility
University of Kentucky



From daemon Tue Mar 21 18:22:05 2000



From: Huggins, Bradley J :      HUGGINBJ-at-BP.com
Date: Tue, 21 Mar 2000 17:59:40 -0600
Subject: Microscope Digital Cameras (Firewire?)

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_____________________________________________________

Karl E. Garsha, Doctoral Student
AOP Fellow
Chief Coordinator-Graduate Student Association
Department of Biological Sciences
University of Wisconsin-Milwaukee
P.O. Box 413
Milwaukee, WI 53201
Office: 459 Lapham Hall
Phone: (414) 229-4316
Mobile: (414) 617-4295
E-Mail: keg-at-csd.uwm.edu

----------
} From: "Karl E. Garsha" {keg-at-csd.uwm.edu}
To: maxwell-at-lec.med.utoronto.ca


Firewire Camera Anyone?
Does anyone know of, or have information on current or upcoming digital
cameras for light microscopy that utilize the Firewire or USB interface for
quick and easy importing of light microscope images through a color digital
camera, for example to a Mac G4 (or even the iMac G3)? Does this
technology exist in a microscope camera? (yet)




From daemon Tue Mar 21 18:52:11 2000



From: RCHIOVETTI-at-aol.com
Date: Tue, 21 Mar 2000 19:33:11 EST
Subject: Re: Microscope Digital Cameras (Firewire?)

Contents Retrieved from Microscopy Listserver Archives
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In a message dated 03/21/2000 5:15:50 PM US Mountain Standard Time,
HUGGINBJ-at-BP.com writes:

{ { Firewire Camera Anyone?
Does anyone know of, or have information on current or upcoming digital
cameras for light microscopy that utilize the Firewire or USB interface for
quick and easy importing of light microscope images through a color digital
camera, for example to a Mac G4 (or even the iMac G3)? Does this
technology exist in a microscope camera? (yet)
} }

The only one I am aware of is the Optronics MagnaFire, which is a cooled
camera with the IEEE-1394 FireWire interface.

Previous versions of the Optronics cameras used a parallel (printer) port
interface so they had to be used on PC's only. It would be worth inquiring
about Mac compatibility. Sorry I don't have the answer to this part...I only
know the MagnaFire has the FireWire interface.

Visit {http://www.optronics.com} and click on "MagnaFire" for specs.

Cheers,

Bob Chiovetti



From daemon Tue Mar 21 20:02:03 2000



From: Valerie Leppert :      vjleppert-at-ucdavis.edu
Date: Tue, 21 Mar 2000 17:48:15 -0800 (PST)
Subject: Film Scanner Recommendations?

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Hi all-

We are in the midst of writing a proposal for a new film scanner and I
am hoping that someone out there can help with a recommendation for a top
of the line film scanner. The major application would be TEM negatives. A
model we are currently looking at is the Nikon LS-4500AF Multi Format Film
Scanner.

If anyone has archived recommendations from previous discussions and could
send them on to me, that would be wonderful.

Thank you,
Valerie Leppert

Dept. of Chem. Eng. and Mat. Sci.
U. of California, Davis

vjleppert-at-ucdavis.edu



From daemon Tue Mar 21 20:18:59 2000



From: Lawrence Kordon :      nikon-at-jagunet.com
Date: Tue, 21 Mar 2000 21:07:37 -0500
Subject: Re: USB or Firewire Cameras

Contents Retrieved from Microscopy Listserver Archives
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I do not work for Optronics, but know a Mac version of Drivers is due
out very
shortly for the Magnafire Camera. For more information, check out....

http://www.optronics.com

Also, Nikon has an SLR based 2.74 megapixel Digital Camera suitable for
most
Microscopy (including bright emission Fluorescence with higher ISO)
called the
D1, based on a N90s Body with a F-mount and Firewire connection. Approx.
$5300

http://www.nikonusa.com/products/detaild1.cfm?id=286

Nikon also has a much less expensive digital camera Coolpix 990 coming
in the
next few weeks. This is a 3.34 million pixel, true resolution camera
with live
NTSC out (for simultaneous real-time video) and USB connectivity with
an
Electronic Shutter Release built-in for around $1000. It too, can handle
most
Microscopy techniques, but is limited in fluorescence by its 100 ISO
rating. It
is a C-mount type camera.

http://www.nikonusa.com/products/detaila.cfm?id=282

Regards,

Lawrence Kordon
Nikon, Inc.
nikon-at-jagunet.com

**I have no corporate affiliation with/nor any financial agreement with
Optronics, Inc. I do however have strong ties with Nikon (as you can
see), but
make very little money from either product ;)



"RCHIOVETTI-at-aol.com"-at-sparc5.microscopy.com wrote:

}
------------------------------------------------------------------------

} The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
} To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
} On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
}
-----------------------------------------------------------------------.

}
} In a message dated 03/21/2000 5:15:50 PM US Mountain Standard Time,
} HUGGINBJ-at-BP.com writes:
}
} { { Firewire Camera Anyone?
} Does anyone know of, or have information on current or upcoming
digital
} cameras for light microscopy that utilize the Firewire or USB
interface for
} quick and easy importing of light microscope images through a color
digital
} camera, for example to a Mac G4 (or even the iMac G3)? Does this
} technology exist in a microscope camera? (yet)
} } }
}
} The only one I am aware of is the Optronics MagnaFire, which is a
cooled
} camera with the IEEE-1394 FireWire interface.
}
} Previous versions of the Optronics cameras used a parallel (printer)
port
} interface so they had to be used on PC's only. It would be worth
inquiring
} about Mac compatibility. Sorry I don't have the answer to this
part...I only
} know the MagnaFire has the FireWire interface.
}
} Visit {http://www.optronics.com} and click on "MagnaFire" for specs.
}
} Cheers,
}



From daemon Wed Mar 22 07:51:43 2000



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Tue, 21 Mar 2000 23:09:26 -0800
Subject: FW: neg stain vs cryo EM plus image averaging

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Dear Karl
I disagree with you, that "UA in particular, will positively stain
proteins". Let start from definition: positively stain object is a dark
object on the light background (therefore stain penetrate/interact with
object making it "stained", dark in the terms of EM); "negative staining"
(NS) is opposite. We have light (stain do not interfere with the sample)
object on the dark background of stain (or dark ring of stain around the
object, depends from stain and technique). The beauty of the NS is that
stain (UA in particular) easily penetrates cavities in the object making
visible fine details (for instance, I was able to recognize variable and
constant domains of the Fab of the IgG molecules as well as domains of the
Fc using UA staining). May be this effect you call "positively staining".
But it is not.

Yes, UA may stain RNA and DNA positively. For this reason UA gives us
"mixed stain" for ribosomes (negative for proteins and positive for RNA
components). The disadvantage UA is its low pH.

As for subject of the current discussion. I think Cryo in general will
give us more correct information than others EM techniques. But, we have
to keep in mind, that in Cryo-technique, to make image relatively contrast,
people should go to very high overfocusing (from half to couple microns, I
believe). For this reason, the best Cryo-results I know, yielded only 2-3
nm resolution (and this is a huge problem in Cryo - to get better
resolution): the same or even worse as for "negative staining" (I expect
1.5 nm resolution for UA). We have to count resolution when compare the
data. I lost the original message from which this discussion was started,
but it seems to me, that difference between NS and Cryo was not so dramatic
and may be comparable if we will count the resolution. In general, I don't
believe any EM data if resolution is not shown. Talking about resolution
is complicated because of the difference between "resolution of the
instrument" (0.14 nm for TEM currently), "physical resolution of the
method" (resolution limit for overfocusing, for instance, radiation damage,
drift, noise/signal ratio etc) and "resolution of the sample" (for
biological samples they are not the same: preparation of the sample may
affect sample's intactness/structure). Cryo is the best for sample
preservation, but it is low in contrast. UA may affect sample's structure
(may not) but the resolution limited only by granularity of stain (in
theory it is a couple angstroms).

As for "averaging" of the images, I am a little bit skeptical about that.
It is great deal if your sample is uniform in shape (symmetry is a great
help too). If your biological sample is functionally flexible (IgG for
instance), averaging will "hide" some interesting details even if you will
distribute images in classes.

In my point of view, it is difficult to extract absolute numbers from EM.
Inside one methodology you could easily compare the data, but it becomes
difficult when you want to compare the data obtained by different
techniques. This is reality of EM. I think Scanning Probe Microscopy is
very promising to obtain the direct measurements on the samples under
physiological conditions (EM is so far from "physiological conditions",
unfortunately). I am so sorry, EM!

} Date: Mon, 20 Mar 2000 16:46:25 -0600
} From: "Karl E. Garsha" {keg-at-csd.uwm.edu}
} Subject: FW: neg stain vs cryo EM plus image averaging
} To: microscopy-at-sparc5.microscopy.com
} X-Mailer: Microsoft Outlook Express for Macintosh - 4.01 (295)
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu
http://www.bol.ucla.edu/~sryazant




From daemon Wed Mar 22 07:51:43 2000



From: A. P. Alves de Matos :      apmatos-at-ip.pt
Date: Qua, 22 Mar 2000 07:13:44 +0000
Subject: Hoechst counterstain

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Dear collegues

I posted a previous message asking for a counterstain for Hoechst 33258, however I fail to mention that I want to use the
counterstain in brignt field ilumination, switching to fluorescence as needed. Something that reveals celular structure like Giemsa
would be OK. Giemsa however does not alow simultaneous staining with Hoechst as far as I can see.

Thanks
Dr. A.P. Alves de Matos
Biologist




From daemon Wed Mar 22 07:51:45 2000



From: Jo Verbeeck :      joverbee-at-ruca.ua.ac.be
Date: Wed, 22 Mar 2000 09:10:12 +0100 (MET)
Subject: Re: EFTEM/GIF on particles

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Hello,

Regarding EFTEM on small particles, this should actually work quite well.
That is, elemental mapping works reasonably if you choose the right
objective apperture. You should simulate the spatial resolution to see
what can be attained under your conditions (HT, Cc, Cs etc).
However it will be very difficult to get real concentration information
out of these images. I'm trying EELS with nanoprobe but so far no succes
(everything gets destroyed before I take a spectrum, allignment is very
very difficult)
Still, focussing remains a problem. Comon practice is to focus at 100eV
and then suppose everything is OK for higher losses. I do not understand
the theory of this technique (blurring by finite slitwidth & Cc?) nor thus
it work well. Any comments on this are welcome.

Jo

*************************************************************
* Jo Verbeeck *
* University of Antwerp *
* Dept. EMAT (Electron Microscopy for Materials Research) *
* e-mail: joverbee-at-ruca.ua.ac.be *
* tel: +32(0)3 218 02 49 *
* fax: +32(0)3 218 02 57 *
*************************************************************

On Tue, 21 Mar 2000, GuessWho wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear all,
}
} I was wondering if anybody was using a GIF filter. We have one mounted
} on a 120 kV TEM and are trying to image small particles (5-10 nm) and
} doing some energy filtering to image different elements in the particles
} (for example gold, silicon, copper, cerium...) . This is not really what
} I would call trivial work and we are still working out the set up
} (window size and positionning for example) in a rather empiric way. The
} main problem is often to get a decent signal in the interesting energy
} region and still keep the windows fairly narrow.
} I would add that we often have to work at low dose because of the beam
} sensitivity of our samples, but I guess our GIF setups can be improved.
} Any suggestion ?
}
} Any general comment on the GIF warmly welcome !
}
} Thanks
}
} Olivier
}
}
}



From daemon Wed Mar 22 07:51:54 2000



From: Steve Chapman :      PROTRAIN-at-CompuServe.COM
Date: Wed, 22 Mar 2000 06:49:33 -0500
Subject: Buying a SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi

A few weeks ago, when our server was working correctly (*??!!*), there were
e-mails talking about the purchase of a new SEM. Now we appear to be back
on line I felt that our ideas may be of use to the mailers?

As part of the consultancy side of our business we purchase SEM for our
clients. This means we are buying instruments regularly and are therefore
probably one of the few professional SEM purchasing organisations?

In our team we have staff who have worked for most of the SEM manufacturers
as demonstrators and application specialists, so we have seen the purchase
game from both sides and of course try to use this to our clients
advantage. Unlike the average microscopist, who probably only buys two
instruments in their career, we have been able over the years to formulate
a purchase plan, a purchasing criteria that we apply to each purchasing
project. In our position we are unable to buy from the nicest salesman or
the normal "gut" feeling, we must be absolutely certain that we are
purchasing the best instrument for our clients applications.

Set out below are the headings from a lecture that I give on instrument
purchase. Only the basic ideas are presented ( I guess if you need more I
shall be attacked by Microscopy Today to give just that) but you will get
the theme. It is important to realise that the specimen chamber of a SEM,
the specimen-detector geometry, determines what we will see. For this
reason you will find that one instrument will do a particular task better
than any other in the price range, you need test specimens that will sort
this out for you. If you have a multi instrument laboratory I always feel
that it is a good idea to have instruments from different manufacturers
optimising the laboratory for optimising a wider range of applications.

"Buying a New Microscope"

How Do You Start?

Collect ALL the brochures and prices
Form a view of the new facilities being made available
If you do not understand any features ASK the salesman
Check through all the users desired facilities?
Determine who will use the instrument now?
Would there be others if you purchased particular accessories?

Formulate a Purchase Specification

Price range - we always look one level higher
Set essential instrument specifications
Set desired instrument specifications give them points and produce a
"desirability assessment"

Why use a consultant?

Only he will without prejudice talk to all interested parties
Interdepartmental feuds could spoil the case
A different questioner provides different answers
Their wide knowledge base may develop additional features
They will have experience producing a "desirability assessment" which often
brings surprises.
They will be experienced with ways of dealing with the sales staff, they
will keep them off your back

How to handle the demonstration? DO NOT LET THEM

Show you how wonderful the alignment procedures are - you will not spend
all day aligning the instrument!
Dominate YOUR demonstration
Take you into totally uninteresting areas of the instrument - you should be
buying because of the image quality
Show their favourite gimmick
Use their own favourite specimen
Take you out of the room when about to load one of your specimens
Take you to a two hour lunch with lots of drink

How to handle the demonstration before you set off

Select a maximum of three specimens that you know really well and that are
important to your laboratory
The specimens must be capable of meaningful low and high magnification
imaging
Develop a demonstration criteria for each specimen
Have a tie break specimen available as suggested by the consultant
Provide each company with an exact demonstration programme

What a consultant would do during a demo

Time the demonstrator to produce images at specific magnifications under
very specific conditions
Encourage the demonstrator (this is not a customer v demonstrator
competition) allow them to try their own ideas with each specimen also,
give them information that you have found to be of importance with your
specimens
Stop the demonstrator taking you away from your standard path - you do wish
to compare each instrument under exactly the same conditions there is no
time for other deviations

After the demo

Layout the results
Compare performance instrument to instrument and time taken to obtain the
results
Produce a short list
Compare the short list with the "desirability assessment"
Research local and national service performance of the best two instrument
manufacturers, and the manufacturers reputations

Finally
Decide upon the instrument that you want, the specification and then haggle

Hope this helps?

Steve Chapman
Senior Consultant
Protrain for Training and Consultancy in EM World Wide
Tel & Fax 44+ 1280 814774
e-mail protrain -at-emcourses.com
web site www.emcourses.com



From daemon Wed Mar 22 07:52:03 2000



From: Griffith, Ed :      EGriffith-at-FEICO.COM
Date: Wed, 22 Mar 2000 05:28:05 -0800
Subject: Cryo-microscopy of solid mineral oils

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dr. Huggins:

The ESEM with a cryostage has been used to observe such things as ice cream
and asphalt/solvent mixtures. The ice cream work was published out of the
Cavendish Labs [THiel, Donald]. The other was a lab experiment.

Hope this helps

Ed G



From daemon Wed Mar 22 08:22:05 2000



From: Dorota Wadowska :      wadowska-at-upei.ca
Date: Wed, 22 Mar 2000 10:13:39 -0400
Subject: TEM-shadow coating

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi!
I have problem obtaining a good replica using a rotary shadow coating
method. Samples are proteins suspended in a Tris, NaCl and CaCl2
buffer. They are absorbed onto a mica and dried in vacuum. I am using
platinum wire (0.1 mm in diameter, 8 cm long) wrapped around tungsten
filament followed by carbon coating. This replica is then
transferred onto the grids. Coating is done in Hitachi HUS 5GB
vacuum evaporator: vacuum 10-6 torr, 20 mA current through the
electrode for 12 sec. The distance from the electrode to the plate
with the samples is 10 cm. The problem is that I get very little
coating (so little that there is no replica) and if I go higher then
20 mA tungsten brakes. I have no problem when I use palladium/gold
wire with same parameters but that gives coarse granulation. This is
the first time I used this method and I run out of ideas how to
improve it.
Thanks in advance for your replies.
Dorota



From daemon Wed Mar 22 08:22:05 2000



From: Terry Cooper :      terry.cooper-at-btinternet.com
Date: Wed, 22 Mar 2000 14:19:20 -0000
Subject: Accu-Edge Blades

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers,

I have just caught up on some of the questions on this server and I think I
can contribute to the blade discussion. As far as I remember Accu-Edge
Blades were re-branded Feather blades sold by Anglia Scientific, a UK
microtome manufacturer in the 1970's and 80's. Anglia were taken over by
Shandon Scientific and in turn they all disappeared into Life Sciences
International. Phil Parker of Anglia I understand is still designing
microtomes for LSI.

In short for Accu-Edge read Feather. We supply these Feather blades as do
other microtome suppliers,

Best regards

Terry Cooper
TAAB Laboratories Equipment Ltd
3 Minerva House, Calleva Park
Aldermaston, Berks, RG7 8NA, England

Tel ++44 118 981 7775 Fax ++44 118 981 7881



From daemon Wed Mar 22 08:32:11 2000



From: Cieslinski, Robert (RC) :      RCCIESLINSKI-at-dow.com
Date: Wed, 22 Mar 2000 09:21:18 -0500
Subject: Announcement - Michigan Local Affiliate Spring Meeting

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Call for Papers
Michigan Microscopy & Microanalysis Society
Spring Meeting

The Michigan Microscopy & Microanalysis Society (Local Affiliate of MSA)
will hold its Spring Meeting May 12th, 2000 at the Genoe Woods Conference
Center in Brighton, Michigan. If you are interest in presenting a microscopy
related paper or attending, please contact Deborah Rothe for more
information, email: drrothe1-at-dow.com .

The society encourages student participation by providing free meeting
registration for students whom present a paper. A cash award is sponsor by
the society vendors for the best student paper.


Robert C. Cieslinski
Michigan Microscopy & Microanalysis


Robert C. Cieslinski
The Dow Chemical Company
Microscopy & Microanalysis
(517) 636-6875
email: rccieslinski-at-dow.com



From daemon Wed Mar 22 18:12:00 2000



From: David Knecht :      knecht-at-uconnvm.uconn.edu
Date: Wed, 22 Mar 2000 10:15:57 -0500
Subject: Re: Microscope Digital Cameras (Firewire?)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} The other Firewire camera I know if currently is the MicroImager.
} Check out http://www.qimaging.com/ Dave



}
} The only one I am aware of is the Optronics MagnaFire, which is a cooled
} camera with the IEEE-1394 FireWire interface.
}
} Previous versions of the Optronics cameras used a parallel (printer) port
} interface so they had to be used on PC's only. It would be worth inquiring
} about Mac compatibility. Sorry I don't have the answer to this part...I only
} know the MagnaFire has the FireWire interface.
}
} Visit {http://www.optronics.com} and click on "MagnaFire" for specs.
}
} Cheers,
}
} Bob Chiovetti

--

************************************************************

Dr. David Knecht
Department of Molecular and Cell Biology
University of Connecticut
75 N. Eagleville Rd. U-125
Storrs, CT 06269
Knecht-at-uconnvm.uconn.edu
860-486-2200 860-486-4331 (fax)
home page: http://www.sp.uconn.edu/~mcbstaff/knecht/knecht.html
************************************************************



From daemon Wed Mar 22 18:12:01 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Wed, 22 Mar 2000 07:23:13 -0600
Subject: Re: Film Scanner Recommendations?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I would suggest that you look at UMAX Powerlook III. It is a flatbed scanner
with built in transparency adapter, so you can scan reflective and transmissive
media. Its resolution is 1200x2400 pixels. This should handle most anything
you might have. I use this scanner all the time. They are about $1100 these days.
For super high quality 35mm scanning, I use the Polaroid SprintScan 35+. It
does 2700 dpi, D=3.4. It runs about $1500. For medium format and 4x5"
transparent media, I use the Polaroid SprintScan 45. It runs about $4500 but
will handle 35mm and 6x6cm media.

I would not recommend the Nikon.

gary g.


At 07:48 PM 3/21/00 , you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Wed Mar 22 18:12:01 2000



From: Gerroir, Paul J :      Paul.Gerroir-at-crt.xerox.com
Date: Wed, 22 Mar 2000 10:27:54 -0500
Subject: RE: Film Scanner Recommendations?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Valerie,
I can't comment on the Nikon scanner but I have plenty to say about the
Polaroid Sprintscan 45, most of which is probably best left unsaid! Let me
summarize. We purchased the Polaroid Sprintscan 45 in late 1998 and planned
to operate it on a PC with Windows 98 operating system. Our first unit was
defective and was replaced, however, the trouble shooting process was very
time consuming. Our second unit functioned intermittently. Again, after much
trouble shooting we returned the unit to Polaroid Canada. After having it
tested we were told that there were no problems with the Sprintscan 45. The
unit was returned to us but continued to cause us grief with its erratic
behavior. We spent more time trying to diagnose the problem and even called
in a computer specialist who spoke directly with Polaroid's tech support
personnel in an effort to resolve the problem. In the end we found that the
Sprintscan appears not to like Windows 98. The scanner has been running
sucessfully on an older PC with Windows 95. Why? Has there been an
identified problem associated with Windows 98? If so why aren't customers
alerted? Having this knowledge would have saved us an enormous amount of
time and money. I have been waiting for a comment from Polaroid for well
over a month.

I would be interested in hearing from other Sprintscan users who experienced
similar problems.

Regards,
Paul

Paul J. Gerroir
Microscopy
Materials Characterization
Xerox Research Centre of Canada
2660 Speakman Drive
Mississauga, Ontario L5K 2L1

Phone: (905) 823-7091, ext. 216
FAX: (905) 822-7022
e-mail: paul.gerroir-at-crt.xerox.com

} -----Original Message-----
} From: Valerie Leppert [SMTP:vjleppert-at-ucdavis.edu]
} Sent: Tuesday, March 21, 2000 8:48 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Film Scanner Recommendations?
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi all-
}
} We are in the midst of writing a proposal for a new film scanner and I
} am hoping that someone out there can help with a recommendation for a top
} of the line film scanner. The major application would be TEM negatives. A
} model we are currently looking at is the Nikon LS-4500AF Multi Format Film
} Scanner.
}
} If anyone has archived recommendations from previous discussions and could
} send them on to me, that would be wonderful.
}
} Thank you,
} Valerie Leppert
}
} Dept. of Chem. Eng. and Mat. Sci.
} U. of California, Davis
}
} vjleppert-at-ucdavis.edu
}



From daemon Wed Mar 22 18:12:02 2000



From: Scott Wight :      scott.wight-at-nist.gov
Date: Wed, 22 Mar 2000 10:28:05 -0500
Subject: Re: EDS in Variable Pressure SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Yes, there is a community of researchers involved in the use of EDS in
ESEM (mostly with the Electroscan now Phillips microscope but the work
is not exclusive of other low vacuum SEMs). See the Microscopy and
Microanalysis Meeting Proceedings from the past several years (in
1999-see Wight pg 290, Carlton pg 292). EDS spatial resolution can be
an issue depending on your conditions. Some general rules (to reduce
the scattering and therefore improve the EDS spatial resolution) are to
reduce the beam-gas-path length (shorten the distance the primary
electrons need to travel thru the gas molecules), lower the chamber
pressure (reduce the number of gas molecules in the path), use a less
scattering gas (Helium has already been suggested), and use as high an
accelerating voltage as reasonable. Several corrections have been
suggested for removing the unwanted contributions to the EDS spectrum:
the extrapolation method (already mentioned), beam stop method,
spectral subtraction method, and continuum method. There are also
simulation models that predict scattering by the gas, I am not aware of
any that predict EDS spectra based on a multiphase or multicomponent
system. I highly recommend that you take a typical sample and ask each
of the microscope manufacturers demonstrate imaging and EDS in their
scope.


I have no interest in any microscope or EDS system,
{fontfamily} {param} Times {/param} Certain commercial software, equipment,
instruments, or materials are identified in this report to specify
adequately the experimental procedure. Such identification does not
imply recommendation or endorsement by the National Institute of
Standards and Technology, nor does it imply that the materials or
equipment identified are necessarily the best available for the
purpose. {/fontfamily}

Scott


} ------------------------------------------------------------------------

} The Microscopy ListServer -- Sponsor: The Microscopy Society of
America


} On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html

} -----------------------------------------------------------------------.

}

}

} It seems that nowadays every SEM vendor is offering variable pressure
models, which are conventional SEMs with a plumbing modification that
allows the sample to be at pressures of up to about 4 torr (500 Pa)
while the electron column operates at the conventional high vacuum. I
am very interested in buying a variable pressure SEM with an EDS, but
was recently warned that EDS has very poor spatial resolution in the
variable pressure mode because of the large beam spread due to
electrons scattering off the gas molecules in the sample chamber. Is
this really a significant issue? Do any of you have experience using
EDS with variable pressure SEMs?

} Thanks,



-------------------note: new mailing address----------------------

Scott Wight e-mail: scott.wight-at-nist.gov

NIST 222/A113 W voice: 301-975-3949

100 Bureau Dr STOP 8371 | fax: 301-417-1321

Gaithersburg, MD 20899-8371 \|/ disclaimer: Any opinion
expressed is my own and does not represent those of my employer.


{/x-rich}



From daemon Wed Mar 22 18:12:02 2000



From: =shAf= :      mshaf-at-darkwing.uoregon.edu
Date: Wed, 22 Mar 2000 07:43:40 -0800
Subject: RE: Film Scanner Recommendations?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Valerie writes ...

} We are in the midst of writing a proposal for a new film
} scanner and I am hoping that someone out there can help
} with a recommendation for a top of the line film scanner.
} The major application would be TEM negatives. ...

I'll not knock the Nikon scanner, but I do believe your
ultimate choice should be based on trial. Most film scanners
anticipate normal films and normal exposures ... which addresses
the film's optical density (... defined as Dmax minus Dmin ...
approximately 3 f/stops for every OD unit ...). For normal
negatives this generally doesn't exceed '3', and for normal
positives, rarely exceeds '3.4'. However, the OD for x-ray
films and electron induced exposures are reported to approach
'4'. You should take one of your most problematic and
representative films and test the scanners, paying particular
attention to the detail/noise in the densest areas of the
negative.
I'll also mention ... you need not be limited to dedicated
film scanners. I don't recall the PPI resolution of the Nikon
scanner, but some of the newest flatbeds, together with their
transparency options, I'm sure can approach the the resolution
of the Nikon (e.g., 1600ppi for the new Epson) ... although I
would tend to believe the film scanners are the most likely to
deliver the best recognition for higher Dmax.

cheerios, shAf :o)

{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {}
Michael Shaffer, R.A. - ICQ 210524
Geological Science's Electron Probe Facility - University of Oregon
mshaf-at-darkwing.uoregon.edu - http://darkwing.uoregon.edu/~mshaf/





From daemon Wed Mar 22 18:12:05 2000



From: Dusevich, Vladimir :      DusevichV-at-umkc.edu
Date: Wed, 22 Mar 2000 10:21:28 -0600
Subject: RE: EDS in Variable Pressure SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hans,
Could you please explain the main idea of the
Vip-Quant algorithm. I cannot even imagine
how quantitative analysis could have nearly the
same accuracy as in high-vacuum mode without
a priori knowledge of the composition of
surrounding areas.
Thank you,

Vladimir


Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
3127 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy

} -----Original Message-----
} From: Hans Dijkstra [mailto:hans.dijkstra-at-edax.nl]
} Sent: Tuesday, March 21, 2000 7:54 AM
} To: Everett Ramer
} Cc: Microscopy-at-sparc5.microscopy.com; Joergen Bilde-Soerensen 5802
} Subject: RE: EDS in Variable Pressure SEM
}
}
} Dear Everett,
}
} The extrapolation method (aka Variable Pressure Method)
} described by Dr.
} Bilde-Sorenson has been implemented by EDAX in a software
} feature called
} ViP-Quant. With this method the EDS analysis under Bad-Vacuum
} conditions can
} be done with nearly the same accuracy as under High-Vacuum conditions.
} Although this method was developed with the ESEM microscope
} in mind, it of
} course can be applied to all Bad-Vacuum scanning electron microscopes.
}
} Please contact your local EDAX representative for more
} information and a
} copy of the new ViP-Quant brochure, or request one through
} www.edax.com.
}
} With best regards,
}
} Hans Dijkstra
}




From daemon Wed Mar 22 18:12:09 2000



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Wed, 22 Mar 2000 11:27:43 -0600
Subject: Water purification system

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

We are planning to upgrade our water supply from a reverse-osmosis,
deionization system by adding a "polishing" system. Since our volume
requirements are low, we're considering the Millipore Simplicity Ultrapure
Water System. Specs for this countertop unit are a resistivity of 18.2
megaohms-cm, with total organic content of {15 ppb for the product water.
The water would be used for routine EM use, i.e., making up reagants,
staining, immunolabeling, etc.

My question is: does anyone have any experience with these systems and would
you be willing to share it with us? Please feel free to respond off-line.

Thanks very much.
Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/





From daemon Wed Mar 22 18:12:11 2000



From: Pauline C. Yu :      splene-at-pw.usda.gov
Date: Wed, 22 Mar 2000 10:13:37 -0800 (PST)
Subject: RE: SprintScan+film scanner tips

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have a SprintScan 45, and while we don't work it to the bone, it has
been relatively reliable(We've had it serviced once since it was
purchased, and that was to have the internal motherboard replaced). I'm
sorry to hear that you've had so many problems Paul. We've had ours since
late 97, and it's been scanning consistently on a Win95 machine. It IS
very fussy with SCSI--let this be a warning to everyone. It won't work
with the new fast cards, but won't work with the really old cards either.
We've currently got it on a Adaptec AHA 15XX series SCSI2 card with UW
negotiation. We haven't been able to try it on a Win98 machine because all
of our Win98 machines have SCSI cards which are too fast.
I've heard good things about the Nikon(especially about Digital ICE).
Minolta also has Digital ICE. The SprintScan isn't perfect, but it gets
our job done. I would NOT recommend a flatbed with transparency adapter
for dedicated film scanning tasks--in general graphic arts professionals
agree that if you want to maximize scan quality from film, it's best to
get the dedicated film scanner rather than the multi-option flatbed.
Dedicated film scanners tend to have better bit depth too.

Of course, if money is no object, a drum scanner or an Imacon flextight
scanner will bring you the best images.

Disclaimer--I have no financial stake in any of the aforementioned
corporate entities blah blah blah

***
Pauline Yu
Microscopist Technician
USDA-ARS-WRRC



From daemon Wed Mar 22 18:12:13 2000



From: Seth Grotelueschen :      sethg-at-paxit.com
Date: Wed, 22 Mar 2000 12:59:12 -0600
Subject: Sony (Firewire?)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Sony makes 2 firewire cameras, the DFW-V300 and DFW-V500. Both are c-mount
cameras and can be used on a light microscope. They are priced in a range
of $2000 or so.

Seth Grotelueschen
MIS, Inc.
www.paxit.com
sethg-at-paxit.com



-----Original Message-----



From: Dusevich, Vladimir :      DusevichV-at-umkc.edu
Date: Wed, 22 Mar 2000 13:20:29 -0600
Subject: RE: EDS in Variable Pressure SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hans,
Could you please explain the main idea of the
Vip-Quant algorithm. I cannot even imagine
how quantitative analysis could have nearly the
same accuracy as in high-vacuum mode without
a priori knowledge of the composition of
surrounding areas.
Thank you,

Vladimir


Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
3127 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy

} -----Original Message-----
} From: Hans Dijkstra [mailto:hans.dijkstra-at-edax.nl]
} Sent: Tuesday, March 21, 2000 7:54 AM
} To: Everett Ramer
} Cc: Microscopy-at-sparc5.microscopy.com; Joergen Bilde-Soerensen 5802
} Subject: RE: EDS in Variable Pressure SEM
}
}
} Dear Everett,
}
} The extrapolation method (aka Variable Pressure Method)
} described by Dr.
} Bilde-Sorenson has been implemented by EDAX in a software
} feature called
} ViP-Quant. With this method the EDS analysis under Bad-Vacuum
} conditions can
} be done with nearly the same accuracy as under High-Vacuum conditions.
} Although this method was developed with the ESEM microscope
} in mind, it of
} course can be applied to all Bad-Vacuum scanning electron microscopes.
}
} Please contact your local EDAX representative for more
} information and a
} copy of the new ViP-Quant brochure, or request one through
} www.edax.com.
}
} With best regards,
}
} Hans Dijkstra
}



From daemon Wed Mar 22 18:12:19 2000



From: Michael Coviello :      coviello-at-mae.uta.edu
Date: Wed, 22 Mar 2000 14:43:39 -0600
Subject: TEM-Digital camera recommendations

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Ya'll:
We are looking for a CCD camera for our Philips 430 TEM. We would like
to get the best camera for the best price, e.g., either buying a used
camera or a new non-Gatan camera (Gatan seems to be twice as expensive
as the others). We would be using the camera for bright field and high
resolution TEM of materials (semiconductors) rather than for biological
specimens. Does anyone have a camera they would like to sell/donate or
does anyone have recommendations as to a less-expensive camera that they
know can be used for materials applications.
Thanks, Mike Coviello
Lab Manager
University of Texas -at- Arlington



From daemon Wed Mar 22 18:12:21 2000



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Wed, 22 Mar 2000 10:57:01 -1000 (HST)
Subject: diffusion pump contamination

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I probably have rotary pump oil in my oil vapor diffusion pump (long
story, situation remedied, I hope). My question is, how well do I have to
clean out the diffusion pump? Solvent clean and bake out, or only
wipe down? What are the probable effects of contamination? Will it affect
the vacuum quite a bit?

After overhauling the vacuum system on our Zeiss 10/A TEM because a faulty
anti-suction device allowed (a LOT) of r.p. oil to migrate throughout the
vacuum lines, I am only getting a vacuum of 6 X 10-4 when I should get 5 X
10-5. Either I've got a leak or a contaminated d.p. When I cleaned out
the d.p. I only wiped it out well, but did not wash it with solvent and
bake it out. I am trying to clean it in place rather than desolder the 13
wires (again) to get it all the way out of the scope, and then have to
resolder (again) while lying on my stomach in the dark recesses of the
instrument with glasses that are the wrong focal length... Call me lazy,
if you wish, but I'm trying to find the easiest but effective way to do
this!

Any tips and tricks appreciated!

Aloha,
Tina

http://www.pbrc.hawaii.edu/bemf/microangela
****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************



From daemon Wed Mar 22 18:12:26 2000



From: David_Bell-at-Millipore.com
Date: Wed, 22 Mar 2000 16:09:00 -0500
Subject: Re: Water purification system

Contents Retrieved from Microscopy Listserver Archives
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Hi Randy,

No comment! :-)

David Bell
Scientist
Electron Microscopy Lab
Millipore Corporation
80 Ashby Road
Bedford, MA 01730
(781) 533-2108



From daemon Wed Mar 22 18:12:27 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Wed, 22 Mar 2000 13:40:03 -0600
Subject: RE: Film Scanner Recommendations? Sprintscan 45

Contents Retrieved from Microscopy Listserver Archives
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The Sprintscan 45; some people swear by them, others swear at them.

I swear by mine. But only after much trouble, and what sounds identical
to your experience. Here's what I found:

It really makes no difference as I see it whether one uses Win95 or Win98.
The crux of the problem is the SCSI controller and how it talks to the
scanner. The Sprintscan 35+ and the 45, the Agfa Arcus II, and several
other scanners were totally flaky and crash prone on my system. I too
sent back the Sprintscan 45 only to be told that it was fine. The SCSI
adapter is a key item in this whole picture. Which adapter were/are you
using?

At the time, I was running a FW SCSI system with an Adaptec AHA-2940UW.
The rear plate connector on this host adapter is a 68-pin FW connector.
The scanners are SCSI-I/II and are narrow, not wide....and definitely not fast.
So, in order to run a scanner on a FW connector, one needs a FW--} SCSI-I
cable and (this is the kicker) a high byte terminator on the host adapter
external connector and a standard narrow SCSI terminator at the scanner.
Good luck trying to get this high byte terminator. Adaptec has a part number
for it but I gave up trying to get one from them. Maybe you will be luckier
than I was.

The alternative is to connect to the narrow SCSI bus at the host adapter and
snake that out the rear of the PC using a blank panel interface that accepts
the 50-pin ribbon cable connector and presents a SCSI-II high density
connector at the rear of the computer. This can work and it did with the
Agfa scanner. But it did not work with the Sprintscans. Even when set
for 10MB/s and no negotiation, it still would hang the system. The solution?
I got a Mac G3/266. I still have it and I still use it for all scanning and
photo input.

Very interesting, and totally successful. All scanners work flawlessly on the
Mac's legacy external SCSI bus. I still am running FW SCSI inside for the
disks, but using the external SCSI for scanners, CD-R, CD-RW and 8mm
tape. The easiest way to run the scanners is via a common TWAIN
interface plug-in in Photoshop. My current main flatbed is a UMAX
Powerlook III. I have not tried it on my PC. My current PC is ATA-66
EIDE with an Adaptec AHA-2930 narrow SCSI adapter. It might run
the UMAX and the Polaroid but everything is hooked up to the Mac. With
DAVE on the Mac, all PCs, printers and the Mac talk to one another via TCP/IP
on a 10BaseT LAN. Any node that needs external access gets it via an
ISDN router. So there is no problem transporting files across platforms.
Worst case is a Zip disk.

Any Mac system before the G4 should work fine. The pre-G3 systems are
really cheap now. G3 systems can also be found rather inexpensively.
A simple system is all that one needs. Heavy duty computing is done on the
PC but photo input and scanning is done on the Mac. This solved all of
my problems--and from your discussion, they were the same as you experienced.

Hope this helps.

gary g.


At 09:27 AM 3/22/00 , you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Wed Mar 22 18:12:28 2000



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Wed, 22 Mar 2000 13:48:40 -0800
Subject: TEM-shadow coating

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Sergey,
I appreciate your feedback. It is obvious to me that you are pretty good at
this. First, I was unclear about the message to which I was responding. I
was responding to the subject "bacteriophage question" by way of Nestor.
To my limited knowledge, UA will positively stain proteins-UA is used as
a positive stain in thin section TEM. I understand the concept(s) of
negative staining well, and I appreciate that UA is one of the best negative
stains for high resolution work (small granularity+nice density).
Totally asymmetrical molecules may be 3-D reconstructed. It just takes
a brute force approach involving thousands of micrographs of the molecule of
interest randomly orientated in vitreous ice. Although your point about
symmetry makes good sense-a more symmetrical molecule should yield a higher
resolution reconstruction (require fewer micrographs for the same level of
confidence in a structure).
Your point about intrument limitations is also well recieved.
A quick concept: both TEM negative staining and Scanning Probe (AFM)
studies of protein macromolecules usually involve the use of charged
surfaces to facilitate adherance and/or orientation of the molecules (i.e.
carbon+glow discharge). Some feel that this may distort the shape of the
molecule (and indeed it will depending on the charge).
In the case of Scanning Probe, there are those who would say that the
"quasi-physical" interaction of the probe (presumably in tapping mode for
molecules under physiological conditions) distorts the reconstructed image
of the protein. I'ld like to say "sorry probe", but I don't have enough
confidence in these statements to do so.
Cheers,
Karl G.

----- Original Message -----
} From: Sergey Ryazantsev {sryazant-at-ucla.edu}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Wednesday, March 22, 2000 1:09 AM


Dear Dorota

You have to use such amount of platinum that it will create a small drop of
the melted metal at the end of the "V"-shape tungsten filament. If the
drop will be large, it is very likely that filament will broke. It seems
to me, 8 cm is a huge amount of platinum. Again, what is diameter of the
filament? 20 mA is a tiny current, seems to me, for thermal evaporation.
But, I never work on HUS 5GB, may be they have special setup. As for
granulation, I don't think that platinum will dramatically improve the
quality of your shadowing. Try platinum-palladium at least. May be, you
have to try buffers, which are able to vaporize in vacuum: ammonium acetate
or bicarbonate adjusted by CO2, for instance. For such applications I am
using for many years 50-150 mM ammonium acetate buffer (with some additives
if necessary, magnesium acetate, for instance) and tungsten shadowing by
electron gun. I also use thickness monitor to control shadowing. I also
use one or bi-directional shadowing: it resolves details better. I am
using rotary shadowing only for DNA and not in all cases. If I have any
problems with shadowing, I am using "test-object" - latex spheres to
determine the problem. Sometimes the problem is just wrong angle of
shadowing. If you have further questions, you may contact me off-listServer.

Sergey


} Date: Wed, 22 Mar 2000 10:13:39 -0400
} From: Dorota Wadowska {wadowska-at-upei.ca}
} Subject: TEM-shadow coating
} To: microscopy-at-sparc5.microscopy.com
} Organization: University of P.E.I.
} Priority: normal
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu
http://www.bol.ucla.edu/~sryazant




From daemon Wed Mar 22 18:12:32 2000



From: GANTZ-at-med-biophd.bu.edu
Date: Wed, 22 Mar 2000 17:31:48 -0400 (EDT)
Subject: Removal of Carbon Replica from Platinum-shadowed sample

Contents Retrieved from Microscopy Listserver Archives
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Our specimen was sprayed on to freshly cleaved mica, rotary
shadowed with platinum, and carbon coated. We are having difficulty
removing the carbon replica. Scoring around the edge did not help.
Would anyone have a suggestion? Thankyou.

Donald Gantz
Boston Univ School of Medicine
gantz-at-med-biophd.bu.edu



From daemon Wed Mar 22 18:12:33 2000



From: Stark,Margery :      Margery.Stark-at-ln.ssw.abbott.com
Date: Wed, 22 Mar 2000 16:45:28 -0600
Subject: Skeletal muscle fixation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listservers,
Does anyone have a good method for skeletal muscle fixation for
Transmission Electron Microscopy? We will not be able to use a perfusion
method. A fixative method would also be greatly appreciated.

Many Thanks

Margery Stark
Abbott Laboratories
Department of Microscopy and Microanalysis
D-45M/AP31
200 Abbott Park Rd.
Abbott Park IL 60064-6202



From daemon Wed Mar 22 18:12:36 2000



From: Huggins, Bradley J :      HUGGINBJ-at-BP.com
Date: Wed, 22 Mar 2000 17:19:40 -0600
Subject: RE: Buying a SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Steve Chapman writes:
} If you have a multi instrument laboratory I always feel
} that it is a good idea to have instruments from different manufacturers
} optimising the laboratory for optimising a wider range of applications.
}
Steve, You make some excellent points. However, I believe that, (just as
you state) potential benefits can be had by choosing different manufacturers
- at the same time you are missing some very significant opportunities if
you create a facility with many different manufacturers. Two important,
potential opportunities are: reduced training time and learning curves for
multiple users, and, discounted service contracts from the manufacturer.

For example, we have in the recent past had the benefit of significantly
reduced service contract costs by having multiple instruments from JEOL and
Philips. At one time our research facility here, happened to have four JEOL
EMs. These four SEMs covered three vastly different SEM instrumentation
ranges (and eras) and serviced different applications. They were all very
capable of handling the tasks for which they were purchased, and we were
able to save big bucks each year over the cost of single instrument service
contract prices. In addition we had multiple users who could go from one
instrument to another without any significant additional training (or
retraining) because of the relatively familiar user interface that
accompanied these microscopes.

Just another perspective.
Have Fun,
Brad Huggins

} ----------
} From: Steve Chapman[SMTP:PROTRAIN-at-CompuServe.COM]
} Sent: Wednesday, March 22, 2000 5:49 AM
} To: American EM Soc
} Subject: Buying a SEM
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi
}
} A few weeks ago, when our server was working correctly (*??!!*), there
} were
} e-mails talking about the purchase of a new SEM. Now we appear to be back
} on line I felt that our ideas may be of use to the mailers?
}
} As part of the consultancy side of our business we purchase SEM for our
} clients. This means we are buying instruments regularly and are therefore
} probably one of the few professional SEM purchasing organisations?
}
} In our team we have staff who have worked for most of the SEM
} manufacturers
} as demonstrators and application specialists, so we have seen the purchase
} game from both sides and of course try to use this to our clients
} advantage. Unlike the average microscopist, who probably only buys two
} instruments in their career, we have been able over the years to formulate
} a purchase plan, a purchasing criteria that we apply to each purchasing
} project. In our position we are unable to buy from the nicest salesman or
} the normal "gut" feeling, we must be absolutely certain that we are
} purchasing the best instrument for our clients applications.
}
} Set out below are the headings from a lecture that I give on instrument
} purchase. Only the basic ideas are presented ( I guess if you need more I
} shall be attacked by Microscopy Today to give just that) but you will get
} the theme. It is important to realise that the specimen chamber of a SEM,
} the specimen-detector geometry, determines what we will see. For this
} reason you will find that one instrument will do a particular task better
} than any other in the price range, you need test specimens that will sort
} this out for you. If you have a multi instrument laboratory I always feel
} that it is a good idea to have instruments from different manufacturers
} optimising the laboratory for optimising a wider range of applications.
}
} "Buying a New Microscope"
}
} How Do You Start?
}
} Collect ALL the brochures and prices
} Form a view of the new facilities being made available
} If you do not understand any features ASK the salesman
} Check through all the users desired facilities?
} Determine who will use the instrument now?
} Would there be others if you purchased particular accessories?
}
} Formulate a Purchase Specification
}
} Price range - we always look one level higher
} Set essential instrument specifications
} Set desired instrument specifications give them points and produce a
} "desirability assessment"
}
} Why use a consultant?
}
} Only he will without prejudice talk to all interested parties
} Interdepartmental feuds could spoil the case
} A different questioner provides different answers
} Their wide knowledge base may develop additional features
} They will have experience producing a "desirability assessment" which
} often
} brings surprises.
} They will be experienced with ways of dealing with the sales staff, they
} will keep them off your back
}
} How to handle the demonstration? DO NOT LET THEM
}
} Show you how wonderful the alignment procedures are - you will not spend
} all day aligning the instrument!
} Dominate YOUR demonstration
} Take you into totally uninteresting areas of the instrument - you should
} be
} buying because of the image quality
} Show their favourite gimmick
} Use their own favourite specimen
} Take you out of the room when about to load one of your specimens
} Take you to a two hour lunch with lots of drink
}
} How to handle the demonstration before you set off
}
} Select a maximum of three specimens that you know really well and that are
} important to your laboratory
} The specimens must be capable of meaningful low and high magnification
} imaging
} Develop a demonstration criteria for each specimen
} Have a tie break specimen available as suggested by the consultant
} Provide each company with an exact demonstration programme
}
} What a consultant would do during a demo
}
} Time the demonstrator to produce images at specific magnifications under
} very specific conditions
} Encourage the demonstrator (this is not a customer v demonstrator
} competition) allow them to try their own ideas with each specimen also,
} give them information that you have found to be of importance with your
} specimens
} Stop the demonstrator taking you away from your standard path - you do
} wish
} to compare each instrument under exactly the same conditions there is no
} time for other deviations
}
} After the demo
}
} Layout the results
} Compare performance instrument to instrument and time taken to obtain the
} results
} Produce a short list
} Compare the short list with the "desirability assessment"
} Research local and national service performance of the best two instrument
} manufacturers, and the manufacturers reputations
}
} Finally
} Decide upon the instrument that you want, the specification and then
} haggle
}
} Hope this helps?
}
} Steve Chapman
} Senior Consultant
} Protrain for Training and Consultancy in EM World Wide
} Tel & Fax 44+ 1280 814774
} e-mail protrain -at-emcourses.com
} web site www.emcourses.com
}



From daemon Wed Mar 22 18:12:37 2000



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Wed, 22 Mar 2000 15:26:31 -0800
Subject: Re: neg stain vs cryo EM plus image averaging

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Karl,
As my knowledge on thin sections, UA stains DNA and RNA, lead citrate -
some proteins and OSO4 - some lipids. Sometimes UA (as well as any others
stains) may just penetrate hydrophilic areas in the plastic sections (which
correspondent, usually, with biological material areas). In this case, I
agree, it may be named "positive staining". But in this case UA stain all
areas, which accessible to water, therefore it is not specific "positive"
stain for proteins.

As for 3D reconstruction. This is a subject for long and very interesting
discussion. I know just a few examples of the complete 3D reconstruction
of asymmetrical particles. Mostly, these works comes from Frank's (Spider
program) and Marin van Heel (Imagic program) groups. The resolution on it
is about 2 nm - not so impressive, if you count, that they did 3D on 26 nm
ribosome. Frank's algorithm with random tilted particles (on the support
film) has "dead angle" (between 60 and 90), therefore, the reconstruction
generally speaking is not "complete". Van Hell's algorithm supposed to be
free from such limitation, but it is not enough reconstructions performed
to prove this technique. But, I am very optimistic about that in general.
Both algorithms used "classification" of particles by type. Criteria for
such classification determined by operator, therefore it is possible that
in one class we will count slightly different particles (different shape or
different orientation or both), averaging of these particles will enhance
major features and lose fine details. Combination X-ray analysis and 3D
reconstruction data (for phasing of the X-ray data set at the beginning)
may help in this case. Marat Yusypov's grop did it for ribosome last year
with great success. But, I have to point, using X-ray crystallography you
studied some particular conformation under conditions of crystallization,
which is not necessary correctly reflect the real conformation in solution.

As for Scanning Probe Microscopy, I agree that probe may distort the
sample. To eliminate such problem, people scan the same area in different
directions. If the images are the same, it means, that distortion from
probe at least smaller than observed creatures. SPM may be very successful
for membrane proteins (which are difficult for EM), pore complexes, etc.
It is not necessary to use "modified" surfaces (glow-discharge, etc) for
SPM or "negative staining" (I never use it for NS, the secret is a quality
of the support carbon film). But, I agree with you, Karl that, as any
"microscopy", SPM is limited in some way in their abilities. Sorry, STM.

Karl, thank you so much for such nice discussion.

Sergey.


} Date: Wed, 22 Mar 2000 15:39:32 -0600
} From: Karl Garsha {keg-at-csd.uwm.edu}
} Subject: Re: neg stain vs cryo EM plus image averaging
} To: Microscopy-at-sparc5.microscopy.com, Sergey Ryazantsev {sryazant-at-ucla.edu}
} X-Mailer: Microsoft Outlook Express 5.00.2615.200
} X-MSMail-Priority: Normal
} X-MimeOLE: Produced By Microsoft MimeOLE V5.00.2615.200
}
} Dear Sergey,
} I appreciate your feedback. It is obvious to me that you are pretty good at
} this. First, I was unclear about the message to which I was responding. I
} was responding to the subject "bacteriophage question" by way of Nestor.
} To my limited knowledge, UA will positively stain proteins-UA is used as
} a positive stain in thin section TEM. I understand the concept(s) of
} negative staining well, and I appreciate that UA is one of the best negative
} stains for high resolution work (small granularity+nice density).
} Totally asymmetrical molecules may be 3-D reconstructed. It just takes
} a brute force approach involving thousands of micrographs of the molecule of
} interest randomly orientated in vitreous ice. Although your point about
} symmetry makes good sense-a more symmetrical molecule should yield a higher
} resolution reconstruction (require fewer micrographs for the same level of
} confidence in a structure).
} Your point about intrument limitations is also well recieved.
} A quick concept: both TEM negative staining and Scanning Probe (AFM)
} studies of protein macromolecules usually involve the use of charged
} surfaces to facilitate adherance and/or orientation of the molecules (i.e.
} carbon+glow discharge). Some feel that this may distort the shape of the
} molecule (and indeed it will depending on the charge).
} In the case of Scanning Probe, there are those who would say that the
} "quasi-physical" interaction of the probe (presumably in tapping mode for
} molecules under physiological conditions) distorts the reconstructed image
} of the protein. I'ld like to say "sorry probe", but I don't have enough
} confidence in these statements to do so.
} Cheers,
} Karl G.
}
} ----- Original Message -----
} From: Sergey Ryazantsev {sryazant-at-ucla.edu}
} To: {Microscopy-at-sparc5.microscopy.com}
} Sent: Wednesday, March 22, 2000 1:09 AM
} Subject: FW: neg stain vs cryo EM plus image averaging
}
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Dear Karl
} } I disagree with you, that "UA in particular, will positively stain
} } proteins". Let start from definition: positively stain object is a dark
} } object on the light background (therefore stain penetrate/interact with
} } object making it "stained", dark in the terms of EM); "negative staining"
} } (NS) is opposite. We have light (stain do not interfere with the sample)
} } object on the dark background of stain (or dark ring of stain around the
} } object, depends from stain and technique). The beauty of the NS is that
} } stain (UA in particular) easily penetrates cavities in the object making
} } visible fine details (for instance, I was able to recognize variable and
} } constant domains of the Fab of the IgG molecules as well as domains of the
} } Fc using UA staining). May be this effect you call "positively staining".
} } But it is not.
} }
} } Yes, UA may stain RNA and DNA positively. For this reason UA gives us
} } "mixed stain" for ribosomes (negative for proteins and positive for RNA
} } components). The disadvantage UA is its low pH.
} }
} } As for subject of the current discussion. I think Cryo in general will
} } give us more correct information than others EM techniques. But, we have
} } to keep in mind, that in Cryo-technique, to make image relatively
} contrast,
} } people should go to very high overfocusing (from half to couple microns, I
} } believe). For this reason, the best Cryo-results I know, yielded only 2-3
} } nm resolution (and this is a huge problem in Cryo - to get better
} } resolution): the same or even worse as for "negative staining" (I expect
} } 1.5 nm resolution for UA). We have to count resolution when compare the
} } data. I lost the original message from which this discussion was started,
} } but it seems to me, that difference between NS and Cryo was not so
} dramatic
} } and may be comparable if we will count the resolution. In general, I
} don't
} } believe any EM data if resolution is not shown. Talking about resolution
} } is complicated because of the difference between "resolution of the
} } instrument" (0.14 nm for TEM currently), "physical resolution of the
} } method" (resolution limit for overfocusing, for instance, radiation
} damage,
} } drift, noise/signal ratio etc) and "resolution of the sample" (for
} } biological samples they are not the same: preparation of the sample may
} } affect sample's intactness/structure). Cryo is the best for sample
} } preservation, but it is low in contrast. UA may affect sample's structure
} } (may not) but the resolution limited only by granularity of stain (in
} } theory it is a couple angstroms).
} }
} } As for "averaging" of the images, I am a little bit skeptical about that.
} } It is great deal if your sample is uniform in shape (symmetry is a great
} } help too). If your biological sample is functionally flexible (IgG for
} } instance), averaging will "hide" some interesting details even if you will
} } distribute images in classes.
} }
} } In my point of view, it is difficult to extract absolute numbers from EM.
} } Inside one methodology you could easily compare the data, but it becomes
} } difficult when you want to compare the data obtained by different
} } techniques. This is reality of EM. I think Scanning Probe Microscopy is
} } very promising to obtain the direct measurements on the samples under
} } physiological conditions (EM is so far from "physiological conditions",
} } unfortunately). I am so sorry, EM!
} }
} } } Date: Mon, 20 Mar 2000 16:46:25 -0600
} } } From: "Karl E. Garsha" {keg-at-csd.uwm.edu}
} } } Subject: FW: neg stain vs cryo EM plus image averaging
} } } To: microscopy-at-sparc5.microscopy.com
} } } X-Mailer: Microsoft Outlook Express for Macintosh - 4.01 (295)
} } }
} } } ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } -----------------------------------------------------------------------.
} } }
} } }
} } }
} } }
} } } _____________________________________________________
} } }
} } } Karl E. Garsha, Doctoral Student
} } } AOP Fellow
} } } Chief Coordinator-Graduate Student Association
} } } Department of Biological Sciences
} } } University of Wisconsin-Milwaukee
} } } P.O. Box 413
} } } Milwaukee, WI 53201
} } } Office: 459 Lapham Hall
} } } Phone: (414) 229-4316
} } } Mobile: (414) 617-4295
} } } E-Mail: keg-at-csd.uwm.edu
} } }
} } } ----------
} } } } From: "Karl E. Garsha" {keg-at-csd.uwm.edu}
} } } To: maxwell-at-lec.med.utoronto.ca
} } } Subject: neg stain vs cryo EM plus image averaging
} } } Date: Mon, Mar 20, 2000, 4:45 PM
} } }
} } }
} } } Dear Karen,
} } } My gut reaction: cryo-EM/3-D reconstruction will yield a much more
} accurate
} } } value with regard to measurement of macromolecular features.
} } } Some "negative" stains, UA in particular, will positively stain
} } } proteins, and so it would be expected that pore size might, in theory, be
} } } over-estimated in neg. stain images. This is a shortcoming of neg.
} staining
} } } as opposed to cryo-EM (if the protein isn't glycosylated). I have no
} doubt
} } } that investigators more experienced than I would argue this point
} however.
} } } The real power of 3-D reconstruction is that it averages many images
} and
} } } determines a structure that is statistically probable...the derived
} } } structure has a certain level of confidence. 3-D reconstruction, as well
} as
} } } 2-D averaging, may be performed on either neg. stained specimens
} (Wilkens,
} } } et. al., Journal of Biological Chemistry, 1999) or cryo-prepared
} specimens
} } } (many important citations...one of my favorites is Boisset, et. al.,
} Journal
} } } of Structural Biology 109, 39-45 (1992).
} } } Either way you need the computer software/hardware and theory
} (Journal
} } } of Structural Biology Vol. 116, Number 1, 1996).
} } } My main point is that a (responsibly) digitally enhanced image should
} } } provide more accurate measurement of macromolecular features than a raw
} } } negative stain photo.
} } } Best Regards,
} } } Karl G.
} } }
} } }
} } } _____________________________________________________
} } }
} } } Karl E. Garsha, Doctoral Student
} } } AOP Fellow
} } } Chief Coordinator-Graduate Student Association
} } } Department of Biological Sciences
} } } University of Wisconsin-Milwaukee
} } } P.O. Box 413
} } } Milwaukee, WI 53201
} } } Office: 459 Lapham Hall
} } } Phone: (414) 229-4316
} } } Mobile: (414) 617-4295
} } } E-Mail: keg-at-csd.uwm.edu
} } }
} } }
} } _____________________________________
} }
} } Sergey Ryazantsev Ph. D.
} } Electron Microscopy
} } UCLA School of Medicine
} } Department of Biological Chemistry
} } Box 951737
} } Los Angeles, CA 90095-1737
} }
} } Phone: (310) 825-1144
} } Pager: (310) 845-0248
} } FAX (departmental): (310) 206-5272
} } mailto:sryazant-at-ucla.edu
} } http://www.bol.ucla.edu/~sryazant
} }
} }
} }
} }
}
}
_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu
http://www.bol.ucla.edu/~sryazant




From daemon Wed Mar 22 18:12:39 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Wed, 22 Mar 2000 16:15:36 -0600
Subject: Re: Microscope Digital Cameras (Firewire?)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I thank you as well for your knowledge and insight, Dr. Ryazanstev. I've
enjoyed this discussion.
-Karl
----- Original Message -----
} From: Sergey Ryazantsev {sryazant-at-ucla.edu}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Wednesday, March 22, 2000 5:26 PM


At 05:59 PM 3/21/00 , you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

It exists. But...and this is a big but....what performance do you seek?
Most of these, at least from what I have seen, are not industrial strength.
They are more consumer- than research-grade units. But maybe this
will satisfy your needs. The higher quality units tend to use SCSI from
what I have seen and used.

gary g.



From daemon Thu Mar 23 09:39:30 2000



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Wed, 22 Mar 2000 20:07:57 -0500
Subject: TEM Pt/C replicas not releasing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Donald Gantz wrote:
===============================================
Our specimen was sprayed on to freshly cleaved mica, rotary shadowed with
platinum, and carbon coated. We are having difficulty removing the carbon
replica. Scoring around the edge did not help. Would anyone have a
suggestion? Thankyou.
===============================================
There are probably as many different ways to approach this kind of problem
as there are people doing platinum replicas! The most reliable method we
have ever discovered is to use a 1% aqueous polyacrylic acid solution,
deposited as one drop on the surface with the stubborn replica. Twenty four
hours later, the drop has spread, the water has evaporated leaving a thin
but very tough PAA film. Then with something sharp (e. g. scalpel blade,
but wear eye protection for this!), slide the blade edge underneath the edge
of the PAA film, and if you have the "art", it will literally just "pop off"
. Now you have the tough PAA layer and the carbon coating, and where it
once rested on the mica, should now be a corresponding area free of replica.

Next the PAA is floated on a surface of water, carbon side up, and again, do
other things and come back 24 hours later. The PAA will have all dissolved
into the water, leaving the carbon/Pt film floating on the surface of the
water. The film is then picked up on grids as you would any other floating
film.

Note: Patience of clearly a virtue, be sure to give it the full 24 hours or
your grids will be PAA contaminated, with a major loss of contrast. Use a
deep petri dish for this so that there is sufficient volume of water to
efficiently dissolve the PAA.

If this sounds too complicated and you don't have patience, there is an
alternative we also use, it involves the use of Victawet® for EM (see our
URL
http://www.2spi.com/catalog/spec_prep/victawet.html

The Victawet is evaporated from a tungsten basket (see website instructions)
and a very thin layer of the release agent is deposited onto the mica (or
glass slide). Then apply your samples, shadow with Pt/C and the replica now
is almost guaranteed to float off on the first try.

One note: The "better" the grade of mica, I am told, the easier is the
release of such films. Grade V1 mica supposedly releases easier and that
might be because there are fewer cleavage steps. We have not tested that
theory ourselves so on that there can be no guarantees.....but it does make
sense. If you are not familiar with the different mica "grades", see URL
http://www.2spi.com/catalog/submat/chart.html

Disclaimer: SPI Supplies is a supplier of Victawet for Electron Microscopy
and mica substrate materials.

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.spi.cc
########################
============================================



From daemon Thu Mar 23 09:39:32 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Wed, 22 Mar 2000 17:21:14 -0600
Subject: Leaf Microlumina Digital Camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have one of these for sale. I may have two. These are specifically
for microscopy. At highest resolution, they take a 28MB TIF file.
This is as high of resolution I have ever seen. Interface is SCSI-II
small sized narrow-SCSI connector.



From daemon Thu Mar 23 09:39:46 2000



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Wed, 22 Mar 2000 20:44:54 -0800
Subject: Removal of Carbon Replica from Platinum-shadowed sample

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Donald

For platinum: try 1-10% aqueous HF. Use it instead water to float replica.
Insert mica slowly. Pickup replica on the grid and rinse with water. Use
corrosion-resistant tweezers. Do not immerse grids into HF solution if it
is copper. You have to do it fast to avoid corrosion of the grid. I was
using copper grids with carbon perforated (holey) film. Perhaps, carbon
protected grid form HF. This is easiest way for platinum shadowing.

You may use even 50% HF - it did not affect platinum shadowing as well as
carbon. Be careful, HF is extremely corrosive, avoid direct contact and
vapors.

If you have questions, you may contact to me off-line.

Good luck!

Sergey

} Date: Wed, 22 Mar 2000 17:31:48 -0400 (EDT)
} From: "GANTZ-at-med-biophd.bu.edu"-at-sparc5.microscopy.com
} Subject: Removal of Carbon Replica from Platinum-shadowed sample
} To: MICROSCOPY-at-sparc5.microscopy.com
} X-VMS-To: MICROSCOPY-at-MSA.MICROSCOPY.COM
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu
http://www.bol.ucla.edu/~sryazant




From daemon Thu Mar 23 09:39:59 2000



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Wed, 22 Mar 2000 22:01:15 -1000 (HST)
Subject: More about diff pump contamination

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I would like to thank those who have replied to my query about diff pump
contamination. Most have asked for more details...

How long have I let the scope pump after cleaning it out and changing diff
pump oil? Just shy of two weeks. The most improvement in vacuum came
within 4 days, then leveled off.

Do I think I got oil into the viewing chamber or column? Yeah,
lots. Lots. Most was on the anode plate, and quite a few droplets on the
liquid nitrogen anticontaminator plate. And even a drop hanging off the
filament! I can see all of you squirming... I cleaned the entire column,
including a couple of parts I had never been into before, and all the vac
lines. I was as thourough as possible, under the circumstances.

I presume I will have a fair amount of vapor around for years to
come. However, the scope operation and resolution aren't bad,
considering. The only thing that keeps me from slitting my wrist is that
we have this great, new, fancy LEO 912 EFTEM that I have been trying to
get all my users trained on, anyway. This means that the old Zeiss 10 can
be my hobby scope. It reminds me of the Volkswagens I used to work
on. Great German engineering!

So one friend thinks that small amounts of rotary pump oil in the
diffusion pump will "burn off" and cease to be a problem, one thinks that
it will only migrate up into the column, and a couple of third party
service people don't have a clue what to expect. No "official" word from
Zeiss/LEO yet. Hoping some of you clever microscopists will have either
direct knowledge or a grand theory backed by thoughtful physics!

Aloha,
Tina

Life's not all fun and Photoshop.
****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************



From daemon Thu Mar 23 09:40:01 2000



From: updancq3f1nkrz5xza-at-aqualung.knoware.nl
Date: Thu, 23 Mar 2000 04:49:05
Subject: Act Now - Reach Thousands of Prospects Monthly

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


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********************************************************
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From daemon Thu Mar 23 09:40:07 2000



From: redancq3f1nkrz5xza-at-molten.co.jp
Date: Thu, 23 Mar 2000 04:12:21
Subject: Special Offer - Reach Thousands of Prospects Monthly

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


_/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/

* * M A K I N G M O N E Y * *

How To Reach Thousands Of Prospects Every Month And Make The
Difference Between Success & Failure In Your Online Business

_/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/

As they say, "It's the simple ideas that make money." And this
is SIMPLE & GREAT INCOME!

Our research has found that many people have tried one or more of
the following...

Free Classifieds? They just don't work anymore
Web Site? Takes thousands of surfers
Banners? Expensive and losing their punch
E-Zine? Hope they have a -huge- subscriber list
Search Engines? Forget it, unless you're in the top 20

S O W H A T D O E S W O R K ?

Although often misunderstood, there is one method that has proven
to succeed time-after-time.

E - M A I L M A R K E T I N G ! !

IT'S A FACT... It if you're not using your computer to generate
GOOD INCOME, you're leaving money on the table.

Here's what the experts have to say about E-Mail Marketing:

"A gold mine for those who can take advantage of
bulk e-mail programs" -The New York Times

"E-mail is an incredible lead generation tool"
-Crains Magazine

"Blows away traditional Mailing" -Advertising Age

Here's a potential earnings example: Let's say you have a
product or service that can bring a profit of around $30.
Remember, on the Internet, you can make money 7 days a week, 24
hours a day... even while you sleep, orders come from all over
the world!

Orders
Per Day Weekly Monthly Yearly

1 $ 210 $ 840 $ 10,080
2 420 1,680 20,160
3 630 2,520 30,240
5 1,050 4,200 50,400
10 2,100 8,400 100,000
15 3,150 12,600 151,200

The way to reach thousands of people, generate orders and build
wealth is person-to-person direct.

1. How do you find the millions of people on the Internet?

2. What are you going to tell them when you do reach them?


HERE'S THE ANSWER TO QUESTION #1

M I L L I O N S V O L U M E 9

***New - 10 Million addresses - Just Released***

The cleanest, most comprehensive e-mail address list in the
world! We're proud to offer it.

O N E O F A K I N D

This is a first. No one has gone to the work it takes to produce
an e-mail address list of this quality.

Here's how we prepare our e-mail lists:

1. We clean and eliminate all duplicates.

2. Next, we use a filter list of 400+ words/phrases to clean even
more. No address with inappropriate or profane wording survive!

3. Then we used our private database of thousands of known
"extremists", opposed to commercial e-mail, and kicked off every
one we could find.

4. And finally, we sorted the list into easy-to-manage
packets of 20,000 addresses in a simple text (.txt) format.

5. All domains have been verified as valid.

WHAT DID WE END UP WITH?

Volume 9... 10 Million Addresses Strong!

An address list so clean you'll want to start mailing today!


N O B R A G - J U S T F A C T

With our super clean e-mail address lists you'll send less...and
get better results...

* Y O U G E T W H A T Y O U P A Y F O R *

Our NEW 10 Million, Volume 9, address list CD will result in:

* Higher Response Rates
* Higher Sales Conversion Ratios
* More Receptive prospects; Less Flames & Non-Buyers.
* Less Contact With Anti-Commerce Radicals & Extremists.

Remember that potential income chart at the beginning of
this message? Can you imagine the kind of money you could
make if you mailed one million pieces and sold only one
tenth (.01%) of one percent? You do the math, you'll be
amazed!

We've been in the list brokerage business for over 5 years and
we've never compromised on quality. We won't release any address
list until it passes our "high standards" test.

This is not a rental list that is restricted to a one-time
mailing. You are purchasing an e-mail address list for your own
personal mailings and may use it over-and-over.

DON'T HESITATE on this offer or you will miss out on the least
expensive, legal and most effective way to market... PERIOD!

_/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/

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From daemon Thu Mar 23 09:40:09 2000



From: Robert H. Olley :      r.h.olley-at-reading.ac.uk
Date: Thu, 23 Mar 2000 10:13:53 +0000 (GMT Standard Time)
Subject: Re: Removal of Carbon Replica from Platinum-shadowed sample

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Donald,

This sounds very like the problem we had removing polymer single crystals
deposited on mica. The heavy metal tends to "key" to the mica, as it also
does if one is shadowing a bulk polymer specimen directly. What we do in
both cases is to put on a tiny amount of carbon first, about one-tenth of
the thickness of the main carbon film.

If the chemistry of your specimen allows, one might spray onto a
polystyrene surface, and then dissolve the PS with butanone.

+------------------------------------------------------------------------+
| Robert H.Olley Phone: |
| J.J.Thomson Physical Laboratory {direct line +44 (0) 118 9318572 |
| University of Reading {University internal extension 7867 |
| Whiteknights Fax +44 (0) 118 9750203 |
| Reading RG6 6AF Email: R.H.Olley-at-reading.ac.uk |
| England URL: http://www.reading.ac.uk/~spsolley |
+------------------------------------------------------------------------+

On Wed, 22 Mar 2000 GANTZ-at-med-biophd.bu.edu-at-sparc5.microscopy.com wrote:

} Our specimen was sprayed on to freshly cleaved mica, rotary
} shadowed with platinum, and carbon coated. We are having difficulty
} removing the carbon replica. Scoring around the edge did not help.
} Would anyone have a suggestion? Thankyou.
}
} Donald Gantz
} Boston Univ School of Medicine
} gantz-at-med-biophd.bu.edu
}




From daemon Thu Mar 23 09:40:37 2000



From: jim :      jim-at-proscitech.com.au
Date: Thu, 23 Mar 2000 21:52:36 +1000
Subject: RE: Removal of Carbon Replica from Platinum-shadowed sample

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


More chemical can access the replica if the replica is scored into 3mm squares
- which is required for TEM anyway. A backing of wax or plastic is useful if
the replica tends to fall apart - I don't expect that this would happen on a
mica surface.
For separating and cleaning use a spotting plate or a small watchglass within a
Petrie dish. I suggest to immerse the mica (or replicas) alternating in a
solution of chromic acid (made up from Pot dichromate) and then concentrated
household bleach - with a water rinse in between. Leave the mica and later the
specimen in those solutions for several hours or over night. 2 periods in both
solutions would be minimal for most tissues, but in your case, once the replica
has separated, the actual cleaning should be minimal.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Thursday, March 23, 2000 7:32 AM,
"GANTZ-at-med-biophd.bu.edu"-at-sparc5.microscopy.com
[SMTP:"GANTZ-at-med-biophd.bu.edu"-at-sparc5.microscopy.com] wrote:
}
} Our specimen was sprayed on to freshly cleaved mica, rotary
} shadowed with platinum, and carbon coated. We are having difficulty
} removing the carbon replica. Scoring around the edge did not help.
} Would anyone have a suggestion? Thankyou.
}
} Donald Gantz
} Boston Univ School of Medicine
} gantz-at-med-biophd.bu.edu



From daemon Thu Mar 23 09:40:39 2000



From: Harmon.Stephen-at-epamail.epa.gov
Date: Thu, 23 Mar 2000 08:50:15 -0500
Subject: Re: EDS in Variable Pressure SEM

Contents Retrieved from Microscopy Listserver Archives
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Dear Ramer Everett,

Please see:

Doehne, E., A New Correction Method for High-Resolution Energy-Dispersive
X-ray Analyses in the Environmental Scanniing Electron Microscope. Scanning
1997, Vol. 19, 75-78.

Carlton, R. A., Energy Dispersive X-ray Spectrometry in the Environmental
Scanning Microscope. Microscope 1999, Vol. 47:1, 5-11.

Cheers,

Stephen M. Harmon
Electron Microscopist
United States Environmental Protection Agency
M.S. 681
26 W. Martin Luther King Blvd.
Cincinnati, OH 45268
513.569.7184
Harmon.Stephen-at-epa.gov




|--------+--------------------------}
| | Everett.Ramer-at-ne|
| | tl.doe.gov |
| | |
| | 03/20/2000 05:22|
| | PM |
| | |
|--------+--------------------------}
} ---------------------------------------------------------|
| |
| To: Microscopy-at-sparc5.microscopy.com |
| cc: |
| Subject: EDS in Variable Pressure SEM |
} ---------------------------------------------------------|






------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


It seems that nowadays every SEM vendor is offering variable pressure models,
which are conventional SEMs with a plumbing modification that allows the sample
to be at pressures of up to about 4 torr (500 Pa) while the electron column
operates at the conventional high vacuum. I am very interested in buying a
variable pressure SEM with an EDS, but was recently warned that EDS has very
poor spatial resolution in the variable pressure mode because of the large beam
spread due to electrons scattering off the gas molecules in the sample chamber.
Is this really a significant issue? Do any of you have experience using EDS with
variable pressure SEMs?
Thanks,







From daemon Fri Mar 24 08:46:23 2000



From: koh young ho :      kohyh-at-bio.umass.edu
Date: Thu, 23 Mar 2000 11:25:46 -0500
Subject: buying ultramicrotome

Contents Retrieved from Microscopy Listserver Archives
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Hi,
I am interested in buying an ultramicrotome which is not necessary be a new
one. Does anybody have idea where I should contact?
Thank's in advances.
Sincerely,

Young Ho Koh
NSB program
UMass Amherst, MA 01003
kohyh-at-bio.umass.edu




From daemon Fri Mar 24 08:46:26 2000



From: Leona Cohen-Gould :      lcgould-at-mail.med.cornell.edu
Date: Thu, 23 Mar 2000 13:17:15 -0400
Subject: Re: Skeletal muscle fixation

Contents Retrieved from Microscopy Listserver Archives
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At 4:45 PM -0600 3/22/0, Stark,Margery wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

**************************

I've worked with muscle (skeletal and cardiac) for years. If you can't
perfuse, you need to keep the muscles from contracting down on themselves.
Depending on the size of the muscle to be fixed, it can be handled as a
whole or blunt dissected into small (1-2mm diam) bundles and tied to
applicator sticks before immersing it in fix. It helps if you've boiled
the sticks in water for 10 minutes or so to extract any resins, etc. The
smaller the bundle, the better your initial fixation. You may want to wash
the bundles in bufer with "high" potassium (100mM KCl) to relax it. I fix
in 2.5% glut, 4% paraform. + approx. 0.02% picric acid (2 ml of a sat'd
sol'n in 40 ml of fix) in 0.1 M Na-cacodylate buffer(Ito & Karnovsky J Cell
Bio 89(abstr. 418) 1968). You can keep the 100mM KCl in this too. I would
suggest that you fix for 30 min or so at RT them overnight in the 'fridge.
Wash well, then cut into smaller pieces before osmium. dehydrate, etc as
usual. I like Spurr's resin, but feel free to use your favorite.

Good luck,
Lee

Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Confocal Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175




From daemon Fri Mar 24 08:46:26 2000



From: Lucille A. Giannuzzi :      lag-at-mail.ucf.edu
Date: Thu, 23 Mar 2000 11:52:20 -0500
Subject: job openning - need immediately!

Contents Retrieved from Microscopy Listserver Archives
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ADVERTISEMENT

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Advanced Materials Processing and Analysis Center
(AMPAC)

The Advanced Materials Processing and Analysis Center (AMPAC) is seeking
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--------------------------------------------------------------------
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********************************************************************




From daemon Fri Mar 24 08:46:29 2000



From: Michael Coviello :      coviello-at-mae.uta.edu
Date: Thu, 23 Mar 2000 12:10:38 -0600
Subject: TEM-Digital camera recommendations-an apology

Contents Retrieved from Microscopy Listserver Archives
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Hi Ya''ll:
In reference to my previous message:
I'm sorry I might have given the impression that I feel the Gatan
digital camera is overpriced...We have lots of Gatan equipment and are
extremely happy with it. Maybe I should have said: we would like to
know what are the lower-priced options for a CCD camera for a Philips
430 (without singling out Gatan). I am sorry for any offense this may
have caused.
Regards, Mike Coviello
University of Texas -at- Arlington



From daemon Fri Mar 24 08:46:29 2000



From: Hans Dijkstra :      hans.dijkstra-at-edax.nl
Date: Thu, 23 Mar 2000 19:19:11 +0100
Subject: RE: EDS in Variable Pressure SEM

Contents Retrieved from Microscopy Listserver Archives
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Dear Vladimir,

The main idea of the ViP-Quant procedure is rather straight-forward and
elegant. It is based on the phenomenon that as the pressure increases the
size of the skirt effect remains fairly constant, although the number of
skirt electrons increases, and thus the contribution of the skirt-effect to
the EDS signal increases. This intensity increase is linear with the
pressure.

This is valid for low- to medium pressures, or at a higher (ESEM) pressure
with a very short free-path length of the skirt electrons, since in both
cases you can assume the skirt electrons have scattered only once. At higher
pressures with longer working distances these assumptions are no longer
fully valid.

So to do an EDS analysis you take 2 measurements at different pressures, the
low-pressure one at a pressure where you can just avaid charging, and a
high-pressure one at at least twice that pressure. The measured intensities
of all elements are then plotted as a function of pressure, and extrapolated
to pressure zero, i.e. high vacuum. The extrapolated results will be very
close to the results hat would have been obtained directly if the sample
could have been analyzed at high vacuum conditions.

Of course anyone can do this plotting and extrapolation manually, that is:
if your EDS software allows you to enter intensities manually! The only
thing the EDAX ViP-Quant is offering as an extra is that the software does
it automatically for you. There are no proprietary miracles involved, EDAX
has just listened carefully to what science has to offer....

Best regards,

Hans Dijkstra

Disclaimer: The above is my personal opinion, and not necessarily EDAX's.
-------------------------------------------------------------
EDAX Europe www.edax.com
Ringbaan Noord 103 Tel.: +31-13-5364000
P.O. Box 4144 Fax.: +31-13-5356279
5004 JC Tilburg E-mail: hans.dijkstra-at-edax.nl
the Netherlands
-------------------------------------------------------------

} -----Original Message-----
}
} From: "Dusevich, Vladimir", INTERNET:DusevichV-at-umkc.edu
} To: Everett Ramer, INTERNET:Everett.Ramer-at-netl.doe.gov
} "'Hans Dijkstra'", INTERNET:hans.dijkstra-at-edax.nl
}
} CC: Joergen Bilde-Soerensen 5802, INTERNET:j.bilde-at-risoe.dk
} [unknown], INTERNET:Microscopy-at-sparc5.microscopy.com
}
} Date: 3/22/00 4:35 PM
}
} RE: RE: EDS in Variable Pressure SEM
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hans,
} Could you please explain the main idea of the
} Vip-Quant algorithm. I cannot even imagine
} how quantitative analysis could have nearly the
} same accuracy as in high-vacuum mode without
} a priori knowledge of the composition of
} surrounding areas.
} Thank you,
}
} Vladimir
}
}
} Vladimir M. Dusevich, Ph.D.
} Electron Microscope Lab Manager
} 3127 School of Dentistry
} 650 E. 25th Street
} Kansas City, MO 64108-2784
}
} Phone: (816) 235-2072
} Fax: (816) 235-5524
} Web: http://www.umkc.edu/dentistry/microscopy
}
} } -----Original Message-----
} } From: Hans Dijkstra [mailto:hans.dijkstra-at-edax.nl]
} } Sent: Tuesday, March 21, 2000 7:54 AM
} } To: Everett Ramer
} } Cc: Microscopy-at-sparc5.microscopy.com; Joergen Bilde-Soerensen 5802
} } Subject: RE: EDS in Variable Pressure SEM
} }
} }
} } Dear Everett,
} }
} } The extrapolation method (aka Variable Pressure Method)
} } described by Dr.
} } Bilde-Sorenson has been implemented by EDAX in a software
} } feature called
} } ViP-Quant. With this method the EDS analysis under Bad-Vacuum
} } conditions can
} } be done with nearly the same accuracy as under High-Vacuum conditions.
} } Although this method was developed with the ESEM microscope
} } in mind, it of
} } course can be applied to all Bad-Vacuum scanning electron microscopes.
} }
} } Please contact your local EDAX representative for more
} } information and a
} } copy of the new ViP-Quant brochure, or request one through
} } www.edax.com.
} }
} } With best regards,
} }
} } Hans Dijkstra
} }
}
}
}
}
}
}
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} From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu}
} To: "'Hans Dijkstra'" {hans.dijkstra-at-edax.nl} ,
} Everett Ramer
} {Everett.Ramer-at-netl.doe.gov}
} Cc: Microscopy-at-sparc5.microscopy.com,
} Joergen Bilde-Soerensen 5802
} {j.bilde-at-risoe.dk}
} Subject: RE: EDS in Variable Pressure SEM
} Date: Wed, 22 Mar 2000 10:21:28 -0600
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From daemon Fri Mar 24 08:46:30 2000



From: Mei Lie Wong :      wong-at-msg.ucsf.edu
Date: Thu, 23 Mar 2000 10:37:55 -0800
Subject: Re: Removal of Carbon Replica from Platinum-shadowed sample

Contents Retrieved from Microscopy Listserver Archives
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Donald -

I sometimes found this and never had a great explanation of why it
happened. I would make sure that your bell jar is clean, i.e.free from
oil. I did find that the thickness of the carbon sometimes mattered. If
it was too thick it shattered. Too thin you cannot see it or it won't stay
together. Sometimes in between it would not float off.

Good luck.

ML
}
}
} Our specimen was sprayed on to freshly cleaved mica, rotary
} shadowed with platinum, and carbon coated. We are having difficulty
} removing the carbon replica. Scoring around the edge did not help.
} Would anyone have a suggestion? Thankyou.
}
} Donald Gantz
} Boston Univ School of Medicine
} gantz-at-med-biophd.bu.edu

Mei Lie Wong
Electron Microscope Laboratory
Department of Biochemistry
HHMI-UCSF
Ph. 415-476-4441 Fax 415-476-1902
http://util.ucsf.edu/agard/wong/index.html
email wong-at-msg.ucsf.edu



From daemon Fri Mar 24 08:46:30 2000



From: Leona Cohen-Gould :      lcgould-at-mail.med.cornell.edu
Date: Thu, 23 Mar 2000 15:19:48 -0400
Subject: Re: buying ultramicrotome

Contents Retrieved from Microscopy Listserver Archives
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At 11:25 AM -0500 3/23/0, koh young ho wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

*****************
for used instruments in the NY Metropoitan are you can try:
M.O.C. at (914)268-6450
and
Marcus Meyerhoff (I'lm not sure I've spelled that correctly) at (732) 747-6228

Lee




From daemon Fri Mar 24 08:46:31 2000



From: George Laing :      scisales-at-ngscorp.com
Date: Thu, 23 Mar 2000 14:16:52 -0500
Subject: RE: Film Scanner Recommendations?

Contents Retrieved from Microscopy Listserver Archives
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Subject: RE: Film Scanner Recommendations?

My name is George Laing from National Graphic Supply. NGS is a vendor of
electronic imaging and traditional photographic products to scientific
markets.
Our customers have had excellent results scanning TEM negatives with the
Agfa Duoscan T2500 scanner. The T2500 looks like a traditional "flatbed"
scanner but utilizes a unique "Twin Plate" design similar to a negative
carrier, that eliminates the use of glass in the film holder. This
eliminates Newton rings,dust on the scanner glass, etc.
Optical resolution is 2500x2500ppi(maximum resolution 5000x5000), Dmax is
3.5. The T2500 has Apochromatic optics and also includes glassless film
holders for 35mm, 120/220 and 4x5 films. Also included is a glass drawer
for transparent originals up to 8"x10". Agfa's Fotolook software is
included for Mac and Windows, interface is SCSI. In addition, the T2500
will also scan reflective originals up to 8.5" x 14".
There are three models in the Duoscan family ranging from
$750 to $5175 list price.
I can provide sample output and literature for anyone who wishes to
contact me directly or visit
http://www.agfahome.com/product/CatProd_DisplayPublic.html?id=3115

George Laing
National Graphic Supply
E-mail: scisales-at-ngscorp.com
Phone: (800) 223-7130 X3109 USA



Valerie,
I can't comment on the Nikon scanner but I have plenty to say about the
Polaroid Sprintscan 45, most of which is probably best left unsaid! Let me
summarize. We purchased the Polaroid Sprintscan 45 in late 1998 and planned
to operate it on a PC with Windows 98 operating system. Our first unit was
defective and was replaced, however, the trouble shooting process was very
time consuming. Our second unit functioned intermittently. Again, after much
trouble shooting we returned the unit to Polaroid Canada. After having it
tested we were told that there were no problems with the Sprintscan 45. The
unit was returned to us but continued to cause us grief with its erratic
behavior. We spent more time trying to diagnose the problem and even called
in a computer specialist who spoke directly with Polaroid's tech support
personnel in an effort to resolve the problem. In the end we found that the
Sprintscan appears not to like Windows 98. The scanner has been running
sucessfully on an older PC with Windows 95. Why? Has there been an
identified problem associated with Windows 98? If so why aren't customers
alerted? Having this knowledge would have saved us an enormous amount of
time and money. I have been waiting for a comment from Polaroid for well
over a month.

I would be interested in hearing from other Sprintscan users who experienced
similar problems.

Regards,
Paul

Paul J. Gerroir
Microscopy
Materials Characterization
Xerox Research Centre of Canada
2660 Speakman Drive
Mississauga, Ontario L5K 2L1

Phone: (905) 823-7091, ext. 216
FAX: (905) 822-7022
e-mail: paul.gerroir-at-crt.xerox.com

} -----Original Message-----
} From: Valerie Leppert [SMTP:vjleppert-at-ucdavis.edu]
} Sent: Tuesday, March 21, 2000 8:48 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Film Scanner Recommendations?
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi all-
}
} We are in the midst of writing a proposal for a new film scanner and I
} am hoping that someone out there can help with a recommendation for a top
} of the line film scanner. The major application would be TEM negatives. A
} model we are currently looking at is the Nikon LS-4500AF Multi Format Film
} Scanner.
}
} If anyone has archived recommendations from previous discussions and could
} send them on to me, that would be wonderful.
}
} Thank you,
} Valerie Leppert
}
} Dept. of Chem. Eng. and Mat. Sci.
} U. of California, Davis
}
} vjleppert-at-ucdavis.edu
}




From daemon Fri Mar 24 08:46:36 2000



From: Gordon Vrololjak :      gvrdolja-at-nature.Berkeley.EDU
Date: Thu, 23 Mar 2000 11:56:04 -0800 (PST)
Subject: video microscope attachments

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


hello,
I was wondering if I could get a sampling of opinions on the different
attachments to light microscopes, dissecting microscopes to display video
to an external monitor. I've seen a few different products available, and
would like to know any experiences people may have had with different
systems. Any comments are greatly appreciated, and I will happily make a
summary of the information.

Sincerely,
Gordon Vrdoljak.

\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
Gordon Ante Vrdoljak Electron Microscope Lab
ICQ 23243541 http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
gvrdolja-at-nature.berkeley.edu UC Berkeley
phone (510) 642-2085 Berkeley CA 94720-3330
fax (510) 643-6207 cell (510) 290-6793



From daemon Fri Mar 24 08:46:38 2000



From: Bernard Kestel :      kestel-at-anl.gov
Date: 23 Mar 00 14:10:03 -0600
Subject: Re: Extracting Precipitates With Replicas

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The Microscopy ListServer -- Sponsor: The Microscopy Society of America


The ongoing discussion about replica techniques continues to be
very
educational. I wish to suggest another approach to this analysis
problem.
Occasionally I jet polish foils of metals which contain precipitates.
Certain
acid mixtures seem more "agressive" and preferentially remove the
precipitates. This seems prone to happen when using nitric or perchloric
acid mixtures.
However, a few years ago when I began to use "non-acid"
electrolytes,
precipitates were sometimes thinned beautifully and were still retained
in the thinned foil. I once got a hole in the center of a large
precipitate!
For more information, see Ultramicroscopy, Vol.19,(1986).
Perhaps more research should be funded along this "thread".
A second approach has been to "design" less agressive electrolytes
that rely on hydrochloric acid, for example, which sometimes works for
some materials but is not in The general literature. Within safty
considerations,worthwhile electrolyte improvements may be achieved with a bit of
work! Good luck.

Bernie Kestel
Materials Science Division
Argonne National Laboratory
9700 South Cass Avenue
Argonne, Il., 60439

E-mail: {kestel-at-anl.gov} FAX: (630) 252-4289







From daemon Fri Mar 24 08:46:53 2000



From: Dusevich, Vladimir :      DusevichV-at-umkc.edu
Date: Thu, 23 Mar 2000 15:08:33 -0600
Subject: RE: EDS in Variable Pressure SEM

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"Dusevich, Vladimir"
{DusevichV-at-umkc.edu}
Cc: Microscopy Listserver {microscopy-at-sparc5.microscopy.com}


Hans,
Thank you very much for explanations.
But for me this method will not work -
quite often I analyze wet specimens at
pretty high pressure (5-6 Torr).
Thank you again,

Vladimir

Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
3127 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy

} -----Original Message-----
} From: Hans Dijkstra [mailto:hans.dijkstra-at-edax.nl]
} Sent: Thursday, March 23, 2000 12:19 PM
} To: DusevichV-at-umkc.edu
} Cc: Microscopy Listserver
} Subject: RE: EDS in Variable Pressure SEM
}
}
} Dear Vladimir,
}
} The main idea of the ViP-Quant procedure is rather
} straight-forward and
} elegant. It is based on the phenomenon that as the pressure
} increases the
} size of the skirt effect remains fairly constant, although
} the number of
} skirt electrons increases, and thus the contribution of the
} skirt-effect to
} the EDS signal increases. This intensity increase is linear with the
} pressure.
}
} This is valid for low- to medium pressures, or at a higher
} (ESEM) pressure
} with a very short free-path length of the skirt electrons,
} since in both
} cases you can assume the skirt electrons have scattered only
} once. At higher
} pressures with longer working distances these assumptions are
} no longer
} fully valid.
}
} So to do an EDS analysis you take 2 measurements at different
} pressures, the
} low-pressure one at a pressure where you can just avaid
} charging, and a
} high-pressure one at at least twice that pressure. The
} measured intensities
} of all elements are then plotted as a function of pressure,
} and extrapolated
} to pressure zero, i.e. high vacuum. The extrapolated results
} will be very
} close to the results hat would have been obtained directly if
} the sample
} could have been analyzed at high vacuum conditions.
}
} Of course anyone can do this plotting and extrapolation
} manually, that is:
} if your EDS software allows you to enter intensities
} manually! The only
} thing the EDAX ViP-Quant is offering as an extra is that the
} software does
} it automatically for you. There are no proprietary miracles
} involved, EDAX
} has just listened carefully to what science has to offer....
}
} Best regards,
}
} Hans Dijkstra
}
} Disclaimer: The above is my personal opinion, and not
} necessarily EDAX's.
} -------------------------------------------------------------
} EDAX Europe www.edax.com
} Ringbaan Noord 103 Tel.: +31-13-5364000
} P.O. Box 4144 Fax.: +31-13-5356279
} 5004 JC Tilburg E-mail: hans.dijkstra-at-edax.nl
} the Netherlands
} -------------------------------------------------------------
}
} } -----Original Message-----
} }
} } From: "Dusevich, Vladimir", INTERNET:DusevichV-at-umkc.edu
} } To: Everett Ramer, INTERNET:Everett.Ramer-at-netl.doe.gov
} } "'Hans Dijkstra'", INTERNET:hans.dijkstra-at-edax.nl
} }
} } CC: Joergen Bilde-Soerensen 5802, INTERNET:j.bilde-at-risoe.dk
} } [unknown], INTERNET:Microscopy-at-sparc5.microscopy.com
} }
} } Date: 3/22/00 4:35 PM
} }
} } RE: RE: EDS in Variable Pressure SEM
} }
} }
} }
} --------------------------------------------------------------
} ----------
} } The Microscopy ListServer -- Sponsor: The Microscopy
} Society of America
} } To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} } On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} }
} --------------------------------------------------------------
} ---------.
} }
} }
} } Hans,
} } Could you please explain the main idea of the
} } Vip-Quant algorithm. I cannot even imagine
} } how quantitative analysis could have nearly the
} } same accuracy as in high-vacuum mode without
} } a priori knowledge of the composition of
} } surrounding areas.
} } Thank you,
} }
} } Vladimir
} }
} }
} } Vladimir M. Dusevich, Ph.D.
} } Electron Microscope Lab Manager
} } 3127 School of Dentistry
} } 650 E. 25th Street
} } Kansas City, MO 64108-2784
} }
} } Phone: (816) 235-2072
} } Fax: (816) 235-5524
} } Web: http://www.umkc.edu/dentistry/microscopy
} }
} } } -----Original Message-----
} } } From: Hans Dijkstra [mailto:hans.dijkstra-at-edax.nl]
} } } Sent: Tuesday, March 21, 2000 7:54 AM
} } } To: Everett Ramer
} } } Cc: Microscopy-at-sparc5.microscopy.com; Joergen Bilde-Soerensen 5802
} } } Subject: RE: EDS in Variable Pressure SEM
} } }
} } }
} } } Dear Everett,
} } }
} } } The extrapolation method (aka Variable Pressure Method)
} } } described by Dr.
} } } Bilde-Sorenson has been implemented by EDAX in a software
} } } feature called
} } } ViP-Quant. With this method the EDS analysis under Bad-Vacuum
} } } conditions can
} } } be done with nearly the same accuracy as under
} High-Vacuum conditions.
} } } Although this method was developed with the ESEM microscope
} } } in mind, it of
} } } course can be applied to all Bad-Vacuum scanning electron
} microscopes.
} } }
} } } Please contact your local EDAX representative for more
} } } information and a
} } } copy of the new ViP-Quant brochure, or request one through
} } } www.edax.com.
} } }
} } } With best regards,
} } }
} } } Hans Dijkstra
} } }
} }
} }
} }
} }
} }
} }
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} } Message-ID: {95A711A70065D111B58C00609451555C01CA7552-at-UMKC-MAIL02}
} } From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu}
} } To: "'Hans Dijkstra'" {hans.dijkstra-at-edax.nl} ,
} } Everett Ramer
} } {Everett.Ramer-at-netl.doe.gov}
} } Cc: Microscopy-at-sparc5.microscopy.com,
} } Joergen Bilde-Soerensen 5802
} } {j.bilde-at-risoe.dk}
} } Subject: RE: EDS in Variable Pressure SEM
} } Date: Wed, 22 Mar 2000 10:21:28 -0600
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From daemon Fri Mar 24 08:46:54 2000



From: Earl Weltmer :      earlw-at-pacbell.net
Date: Thu, 23 Mar 2000 13:19:43 -0800
Subject: Dumping of Service Contracts costs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All,

I've heard from several SEM/TEM independent maintenance companies that
some of the manufacturer's have engaged in "price dumping" when
negotiating a service contract.

In other words, if the manufacturer is aware that they have competition

for a service contract they will reduce the contract by as much as forty

percent.

I am all for the free enterprise system, but I thought that when a
contract price is offered it is the same for all customers especially
government contracts that must abide by the GSA rules. GSA rules states

that the price offered is the lowest price for this service. It would
be
illegal for the manufacturer (or anyone) to offer a contract at less
than the cost offered to GSA customers.

I am surprised by this move as our contracts are the same as we abide
by
the GSA rules.

Regards,

Earl Weltmer



From daemon Fri Mar 24 08:46:55 2000



From: Dusevich, Vladimir :      DusevichV-at-umkc.edu
Date: Thu, 23 Mar 2000 15:43:53 -0600
Subject: RE: EDS in Variable Pressure SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


EDS in ESEM is a pretty similar thing to
EDS in SEM if you do not need trace/minor (~1%) element analysis
and use some standard precautions. Some vendors even
sell as an option gaseous detectors which could be
placed close (1mm) to a sample and greatly reduce
a beam scattering. I routinely use EDS at water vapor
pressure up to 6 Torr. I've put an X-ray map taken
in environmental conditions at our web site
http://www.umkc.edu/dentistry/microscopy
If low concentrations are of no interest to you then
you will not see real difference in performance of an
EDS in VP and high vacuum modes, especially if you
do not work with wet specimens which need really high
pressure.


Vladimir


Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
3127 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy

}
}
} It seems that nowadays every SEM vendor is offering variable
} pressure models, which are conventional SEMs with a plumbing
} modification that allows the sample to be at pressures of up
} to about 4 torr (500 Pa) while the electron column operates
} at the conventional high vacuum. I am very interested in
} buying a variable pressure SEM with an EDS, but was recently
} warned that EDS has very poor spatial resolution in the
} variable pressure mode because of the large beam spread due
} to electrons scattering off the gas molecules in the sample
} chamber. Is this really a significant issue? Do any of you
} have experience using EDS with variable pressure SEMs?
} Thanks,
}
}



From daemon Fri Mar 24 08:46:57 2000



From: Scott Robinson :      sjrobin-at-itg.uiuc.edu
Date: Thu, 23 Mar 2000 16:16:36 -0600
Subject: SEM query: aquatic invertebrates

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Some of the K-12 classrooms we serve in our Bugscope
(http://bugscope.beckman.uiuc.edu/) program have expressed interest in
sending us aquatic or marine invertebrates to prepare for their sessions.
What I'd like to know is how I should ask them to prepare the samples for
me (e.g., should I ask them to overnight pondwater or seawater samples in
plastic bottles?), and after that, what should I do with them? I can't
really be sending glutaraldehyde and cacodylate to gradeschool kids, but
I'm assuming I'll want to do some sort of standard
fixation/dehydration/HMDS treatment, presumably on Millipore filters, once
I get samples. Any tips?

Thanks
Scott Robinson




From daemon Fri Mar 24 08:47:02 2000



From: Tom Gore :      togo-at-uvvm.uvic.ca
Date: Thu, 23 Mar 2000 14:54:39 -0800
Subject: Film Scanner Recommendations?

Contents Retrieved from Microscopy Listserver Archives
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} We are in the midst of writing a proposal for a new film scanner and I
} am hoping that someone out there can help with a recommendation for a top
} of the line film scanner. The major application would be TEM negatives. A
} model we are currently looking at is the Nikon LS-4500AF Multi Format Film
} Scanner.
}
} Valerie Leppert
} Dept. of Chem. Eng. and Mat. Sci.
} U. of California, Davis

We use a Polaroid SprintScan 45 for SEM, TEM and other large film but find
that the negatives need to be somewhat tailored to the scanner's needs. We
have great difficulty with very dense or very high contrast negatives,
especially from the TEM. Staining and microscope settings need to be
adjusted to produce negatives that are within the range of the scanner. It
then produces very good results.


_____________________________________
Tom Gore, Advanced Imaging Laboratory
Biology Department, University of Victoria
Box 3020, Station CSC,
Victoria, BC, V8W 3N5 Canada
voice (250) 721-7134 fax (250) 721-7120
web: http://web.uvic.ca/ail/




From daemon Fri Mar 24 08:47:05 2000



From: Bill Chissoe :      wchiss-at-ou.edu
Date: Thu, 23 Mar 2000 17:40:29 -0600
Subject: SEM service

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We are looking for an independant service provider for our JEOL-880 SEM.
They would need to be very familiar with JEOL equipment since the 880 is
a rare bird (the only one in the States, I believe). I believe it's a
1200 TEM column married to 840 electronics. We are located in central
Oklahoma. TIA.

Bill

--
=============================================================
Bill Chissoe III
Electron Microscopist
University of Oklahoma
770 Van Vleet Oval
Norman, Ok. 73019
E-mail: wchiss-at-ou.edu Ph. (405)325-4391
=============================================================




From daemon Fri Mar 24 08:47:09 2000



From: John J. Bozzola :      bozzola-at-siu.edu
Date: Thu, 23 Mar 2000 18:21:34 -0600
Subject: The Image Processing Tool Kit

Contents Retrieved from Microscopy Listserver Archives
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I am looking for a vendor to purchase The Image Processing Tool Kit
by John Russ. I placed an order with Amazon nearly a month ago but
have yet to receive anything. Hmmmm.

Thank you,

John B.

####################################################################
John J. Bozzola, Ph.D., Director
Micro-Imaging and Analysis Center
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
####################################################################



From daemon Fri Mar 24 08:47:13 2000



From: Mark YEADON :      m-yeadon-at-imre.org.sg
Date: Fri, 24 Mar 2000 09:11:27 +0800
Subject: RE: TEM-Digital camera recommendations

Contents Retrieved from Microscopy Listserver Archives
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Mike,

You could check out Soft Imaging Systems' MegaView II at:

http://www.soft-imaging-web.de/ {http://www.soft-imaging-web.de/}

I'd like to hear other recommendations too...

Mark

%%%%%%%%%%%%%%%%%%
Mark Yeadon
Senior Research Fellow
Institute of Materials Research and Engineering
3 Research Link
Singapore 117602

Assistant Professor
Department of Materials Science
National University of Singapore
Singapore 119260

TEL: (+65) 874 8591
FAX: (+65) 872 0785
Email: m-yeadon-at-imre.org.sg {mailto:m-yeadon-at-imre.org.sg}



-----Original Message-----
From: Michael Coviello [SMTP:coviello-at-mae.uta.edu]
Sent: Thursday, March 23, 2000 4:44 AM
To: Microscopy-at-sparc5.microscopy.com
Subject: TEM-Digital camera recommendations


------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
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Hi Ya'll:
We are looking for a CCD camera for our Philips 430 TEM. We would
like
to get the best camera for the best price, e.g., either buying a
used
camera or a new non-Gatan camera (Gatan seems to be twice as
expensive
as the others). We would be using the camera for bright field and
high
resolution TEM of materials (semiconductors) rather than for
biological
specimens. Does anyone have a camera they would like to sell/donate
or
does anyone have recommendations as to a less-expensive camera that
they
know can be used for materials applications.
Thanks, Mike Coviello
Lab Manager
University of Texas -at- Arlington




From daemon Fri Mar 24 08:47:14 2000



From: Susnitzky, David :      david.susnitzky-at-intel.com
Date: Thu, 23 Mar 2000 17:16:49 -0800
Subject: Announcement - N. California Local Affiliate Meeting on March 30t

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N. California Society for Microcsopy

MARCH 30TH MEETING AT GENENTECH
TOPIC: Microscopy and Public Health.

Our two speakers come from the USDA, Agricultural Research Service in
Albany, CA. Robert Mandrell's presentation is titled "Analysis of Human
Pathogens on Food Surfaces by Stereo- and Confocal Micros-copy". Robert is
the Research Leader of the Food Safety and Health Unit at the USDA facility.
Also speaking is De Wood on "Immunolocalization using FESEM and a
Backscatter Detector". De heads up the Microscopy & Imaging Lab at the
USDA.

Please call Greg Lum at 415/338-1339 or Email: "glum-at-sfsu.edu" for dinner
reservations and entree choice by Monday March 27th. The meeting starts at
5:00 p.m. with a beverage reception. The dinner will start at 6:00 pm.

DINNER RESERVATIONS
Entree choice (select one) $20 nonmember, $15 regular members; $8
student members
Menu choices for Genentech meeting on March 30th
[ ] Chicken Parmesan
[ ] Flank Steak with Mushroom Sauce
[ ] Pasta Primavera

Please call Greg Lum at 415/338-1339 or Email: "glum-at-sfsu.edu" for dinner
reservations and entree choice by Monday March 27th. The meeting starts at
5:00 p.m. with a beverage reception. The dinner will start at 6:00 pm.



From daemon Fri Mar 24 08:47:18 2000



From: G. Macdonald :      glenmac-at-u.washington.edu
Date: Thu, 23 Mar 2000 17:52:30 -0800 (PST)
Subject: Re: Film Scanner Recommendations?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


There are two labs on campus with the Agfa Duoscan T2500 scanners. As
stated in the message from National Graphics Supply, they have both a flat
bed and a transparency drawer. They will scan at 16-bit gray scale as
well as in 8-bit and RGB. Weve been very happy with ours, primarily using
it for TEM and SEM negatives, and for 35 mm. One caveat, the 2500 dpi
capability is limited to a 4 inch x 14 inch area (1/2 width of the scan
area). Set-up was simple and no crashes (so far). The software is easy to
learn with identical interfaces for both the standalone application and
the Photoshop compatible plug-in.

We purchased ours locally from a professional photography retailer.

Glen


Glen MacDonald
Research Scientist
Hearing Research Laboratories of the
Virginia Merrill Bloedel Hearing Research Center
Box 35-7923
University of Washington
Seattle, WA 98195-7923
(206) 616-4156
glenmac-at-u.washington.edu




From daemon Fri Mar 24 08:47:21 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Thu, 23 Mar 2000 18:17:31 -0600
Subject: Re: Dumping of Service Contracts costs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hum.... This is very interesting. Government customers are one
thing, non-government customers are another. GSA rules and
principles do not apply to non-government customers. If you
would please cite the FAR that is the basis for your assertion,
this could help a lot to clarify your statement.

I have never heard of "price dumping." But I have heard of
competition. One must keep U.S. government business separate
from others. Even then, how does one mingle them together?
On what basis is this done?

How much of this "competition" is based on multiple unit discounts versus
actual price reduction? And as a consumer, what real difference
does it make? If the consumer can negotiate a good deal, all
the better for the consumer, right?

Mixing GSA into free enterprise is like apples and oranges, so
to speak.

What do you think?

gary g.


At 03:19 PM 3/23/00 , you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Fri Mar 24 08:47:26 2000



From: earlw-at-pacbell.net
Date: Thu, 23 Mar 2000 19:22:06 -0800
Subject: Re: SEM service

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


You are right. The 880 is an "immersion lens" SEM, a very rare animal. There
are only two that I know of: One at OU, the second was at IBM in France but
is now in the back of my office sadly used for parts.

Earl Weltmer

Bill Chissoe wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} We are looking for an independant service provider for our JEOL-880 SEM.
} They would need to be very familiar with JEOL equipment since the 880 is
} a rare bird (the only one in the States, I believe). I believe it's a
} 1200 TEM column married to 840 electronics. We are located in central
} Oklahoma. TIA.
}
} Bill
}
} --
} =============================================================
} Bill Chissoe III
} Electron Microscopist
} University of Oklahoma
} 770 Van Vleet Oval
} Norman, Ok. 73019
} E-mail: wchiss-at-ou.edu Ph. (405)325-4391
} =============================================================



From daemon Fri Mar 24 08:47:27 2000



From: earlw-at-pacbell.net
Date: Thu, 23 Mar 2000 19:23:04 -0800
Subject: Re: Dumping of Service Contracts costs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The Company providing the service does certify to the GSA customer that the
prices given are the same or lower than the prices given to any other entity:
public, government or private.

Earl Weltmer

"Dr. Gary Gaugler" wrote:

} Hum.... This is very interesting. Government customers are one
} thing, non-government customers are another. GSA rules and
} principles do not apply to non-government customers. If you
} would please cite the FAR that is the basis for your assertion,
} this could help a lot to clarify your statement.
}
} I have never heard of "price dumping." But I have heard of
} competition. One must keep U.S. government business separate
} from others. Even then, how does one mingle them together?
} On what basis is this done?
}
} How much of this "competition" is based on multiple unit discounts versus
} actual price reduction? And as a consumer, what real difference
} does it make? If the consumer can negotiate a good deal, all
} the better for the consumer, right?
}
} Mixing GSA into free enterprise is like apples and oranges, so
} to speak.
}
} What do you think?
}
} gary g.
}
} At 03:19 PM 3/23/00 , you wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Hi All,
} }
} } I've heard from several SEM/TEM independent maintenance companies that
} } some of the manufacturer's have engaged in "price dumping" when
} } negotiating a service contract.
} }
} } In other words, if the manufacturer is aware that they have competition
} }
} } for a service contract they will reduce the contract by as much as forty
} }
} } percent.
} }
} } I am all for the free enterprise system, but I thought that when a
} } contract price is offered it is the same for all customers especially
} } government contracts that must abide by the GSA rules. GSA rules states
} }
} } that the price offered is the lowest price for this service. It would
} } be
} } illegal for the manufacturer (or anyone) to offer a contract at less
} } than the cost offered to GSA customers.
} }
} } I am surprised by this move as our contracts are the same as we abide
} } by
} } the GSA rules.
} }
} } Regards,
} }
} } Earl Weltmer



From daemon Fri Mar 24 08:47:27 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Thu, 23 Mar 2000 19:34:30 -0600
Subject: Re: Dumping of Service Contracts costs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


OK....to a GSA customer. What about to other customers? And
what precedence does one GSA contract or schedule have for
other instantiations? They are, in my experience, single point
events. They are not precedence events.

Where is the original thread that spawned this message?

Note, that in my experience, GSA contracts are distinct from other
Federal government contracts. i.e., GSA is one thing, other
gov contracts are another. GSA negotiates rates....they do not
establish them. Therefore, for a particular GSA contract or rate
structure, the rates are pre-negotiated for fed users to adopt
as per their own contracting department. Or, they can use the
GSA schedule and go with that vehicle and its added surcharge.
(Yes, GSA contracts cost more than face value). GSA has two
flavors: negotiated rates (the user does their own contracting) and
negotiated contracts (the user buys into the GSA contract and pays
a surcharge for doing so).

Which flavor are you talking about?

gg

At 09:19 PM 3/23/00 , you wrote:
} The Company providing the service does certify to the GSA customer that the
} prices given are the same or lower than the prices given to any other entity:
} public, government or private.
}
} Earl Weltmer
}
} "Dr. Gary Gaugler" wrote:
}
} } Hum.... This is very interesting. Government customers are one
} } thing, non-government customers are another. GSA rules and
} } principles do not apply to non-government customers. If you
} } would please cite the FAR that is the basis for your assertion,
} } this could help a lot to clarify your statement.
} }
} } I have never heard of "price dumping." But I have heard of
} } competition. One must keep U.S. government business separate
} } from others. Even then, how does one mingle them together?
} } On what basis is this done?
} }
} } How much of this "competition" is based on multiple unit discounts versus
} } actual price reduction? And as a consumer, what real difference
} } does it make? If the consumer can negotiate a good deal, all
} } the better for the consumer, right?
} }
} } Mixing GSA into free enterprise is like apples and oranges, so
} } to speak.
} }
} } What do you think?
} }
} } gary g.
} }
} } At 03:19 PM 3/23/00 , you wrote:
} } } ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } -----------------------------------------------------------------------.
} } }
} } }
} } } Hi All,
} } }
} } } I've heard from several SEM/TEM independent maintenance companies that
} } } some of the manufacturer's have engaged in "price dumping" when
} } } negotiating a service contract.
} } }
} } } In other words, if the manufacturer is aware that they have competition
} } }
} } } for a service contract they will reduce the contract by as much as forty
} } }
} } } percent.
} } }
} } } I am all for the free enterprise system, but I thought that when a
} } } contract price is offered it is the same for all customers especially
} } } government contracts that must abide by the GSA rules. GSA rules states
} } }
} } } that the price offered is the lowest price for this service. It would
} } } be
} } } illegal for the manufacturer (or anyone) to offer a contract at less
} } } than the cost offered to GSA customers.
} } }
} } } I am surprised by this move as our contracts are the same as we abide
} } } by
} } } the GSA rules.
} } }
} } } Regards,
} } }
} } } Earl Weltmer



From daemon Fri Mar 24 08:47:35 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Thu, 23 Mar 2000 22:07:51 -0600
Subject: Re: The Image Processing Tool Kit

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On the very low end A Snappy digitizer and a surveillance
video camera produce what I would consider the minimal
acceptable image. It is good enough for some work I would
say it is comparable to a Polaroid image or slightly worse.

} From a quality per dollar point of view it can't be beat.
I would say it is good enough for collaboration, web publishing
and some publishing.

This set up and 35mm camera should meet all quality needs as
long as you don't need real-time images.
It does not compare to a top of the line dedicated system. But it does
better than anything but a dedicated system.

My best effort to date is:
http://www.couger.com/microscope/onion.jpg
This is the raw image with no enhancement and the enhanced version is:
http://www.couger.com/microscope/onionE.jpg
This version was contrast enhanced and mapped to false color.
The subject is a partially dehydrated onion cell. There are depth
of focus problems and possibly some vibration problems. There
is only 7.5 bits of information in the raw image. I attribute this
to loss of dynamic range in the camera due to age.

This was taken with a Leitz darkfield condenser and a Leitz 63x
0.85 objective with a variable aperture for darkfield work. The
camera was a monochrome RCA surveillance videocron camera
and a Snappy digitizer.

I don't think the microscope end can be improved on much in the
onion image. I still had dynamic range problems. The only solution
I can think of is to take multiple pictures at different light levels and
combine them. A high resolution CCD camera will give better
resolution but I don't think the dynamic range problem will go
away using video cameras. Maybe I will get around to
exposing a 4X5 negative and see what is really there.
But after using digital capture it is an awful lot of trouble.

I am strictly an amateur with a microscope but I do have
professional experience in digital images.

Gordon

Gordon Couger gcouger-at-couger.com

Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00

----- Original Message -----
} From: "Gordon Vrololjak" {gvrdolja-at-nature.Berkeley.EDU}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Thursday, March 23, 2000 1:56 PM


Try going direct:

Reindeer Games, Inc.
235 S. Main St. #201
Gainesville, FL 32601
352.384.1850

http://members.aol.com/foveapro

jcr6-at-aol.com

gg


At 06:21 PM 3/23/00 , you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I am looking for a vendor to purchase The Image Processing Tool Kit by John Russ. I placed an order with Amazon nearly a month ago but have yet to receive anything. Hmmmm.
}
} Thank you,
}
} John B.
}
} ####################################################################
} John J. Bozzola, Ph.D., Director
} Micro-Imaging and Analysis Center
} 750 Communications Drive - MC 4402
} Southern Illinois University
} Carbondale, IL 62901 U.S.A.
} Phone: 618-453-3730
} Fax: 618-453-2665
} Email: bozzola-at-siu.edu
} Web: http://www.siu.edu/departments/shops/cem.html
} ####################################################################



From daemon Fri Mar 24 08:47:45 2000



From: Robin Cross :      r.cross-at-ru.ac.za
Date: Fri, 24 Mar 2000 08:22:39 +0200
Subject: Re: The Image Processing Tool Kit

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi John

} I am looking for a vendor to purchase The Image Processing Tool Kit by
} John Russ.

I believe you can purchase it directly from John Russ.

Rob



Robin H Cross
Director : EM Unit, Rhodes University, Grahamstown, South Africa
R.Cross-at-ru.ac.za
tel: +27 46 603 8168 - fax: +27 46 622 4377
http://www.ru.ac.za/emu/index.htm

** remember that ICEM-15 in 2002 is in Durban, South Africa **



From daemon Fri Mar 24 08:48:07 2000



From: Hans Dijkstra :      hans.dijkstra-at-edax.nl
Date: Fri, 24 Mar 2000 12:32:22 +0100
Subject: RE: EDS in Variable Pressure SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Scott
Some aquatic invertebrates such as gastrotrichs are difficult to fix
for SEM in a life-like relaxed state because aldehyde fixatives
induce violent contraction. A paper by May+ advocates relaxing the
organisms (rotifers in this instance) first by narcosis with procaine,
or similar anaesthetics. However, in our experience gastrotrichs
can sense these anaesthetics, and considerable distortion results.
There is a considerable literature on this topic covering the
properties of EDTA, menthol, osmium tetroxide, etc. etc. for this
purpose, but mostly these solutions are ineffective at least on
gastrotrichs.

We have found sodium azide at ~0.1% to be very effective at
preventing distortion. Gastrotrichs seem not to be irritated by it, but
simply go to sleep. We have usually mounted them for LM in
glycerol, in which they can be preserved and collected for transit
or for subsequent processing and examination. The fixation/
dehydration/ HMDS procedure you suggest would probably be very
effective, but you might also try Ensikat & Barthlott's* approach of
simply examining them in the SEM in the glycerol-wet state, or
after drying them from glycerol under vacuum.

+May, L. (1985) The use of procaine hydrochloride in the
preparation of rotifer samples for counting. Verh. Internat. Verein.
Limnol. 22, 2897-2990
*Ensikat & Barthlott (1993) Liquid substitution - a versatile
procedure for sem specimen preparation of biological-materials
without drying or coating Journal of Microscopy 172, 195-203

Sodium azide is obviously a serious poison, and schoolkids cannot
handle the powder, but would it be acceptable for them to use
0.1% solution under supervision by a teacher? I guess this
depends on local legislation and attitudes, and also on the age and
level of experience and responsibility of the students.

Hope this helps
Chris

Date sent: Thu, 23 Mar 2000 16:16:36 -0600
To: Microscopy-at-sparc5.microscopy.com
} From: Scott Robinson {sjrobin-at-itg.uiuc.edu}


Dear Vladimir,

Under the circumstances you describe you are probably on the edge of the
condition where the virtual composition changes linearly with the pressure,
but this depends on the working distance and the gas that you use. However,
if you still have some pressure range to work with, then making several
measurements at a range of pressures may still allow you to use a non-linear
extrapolation.

The main problem might be that wet specimens often have a thin water film on
the sample, which may adversely affect EDS analysis. Keeping exact control
over sample temperature, pressure and humidity is required to get suitable
EDS conditions.

With best regards,

Hans Dijkstra

-------------------------------------------------------------
EDAX Europe www.edax.com
Ringbaan Noord 103 Tel.: +31-13-5364000
P.O. Box 4144 Fax.: +31-13-5356279
5004 JC Tilburg E-mail: hans.dijkstra-at-edax.nl
the Netherlands
-------------------------------------------------------------

} -----Original Message-----
} From: Dusevich, Vladimir [mailto:DusevichV-at-umkc.edu]
} Sent: Thursday, March 23, 2000 10:09 PM
} To: 'Hans Dijkstra'; Dusevich, Vladimir
} Cc: Microscopy Listserver
} Subject: RE: EDS in Variable Pressure SEM
}
}
} Hans,
} Thank you very much for explanations.
} But for me this method will not work -
} quite often I analyze wet specimens at
} pretty high pressure (5-6 Torr).
} Thank you again,
}
} Vladimir
}
} Vladimir M. Dusevich, Ph.D.
} Electron Microscope Lab Manager
} 3127 School of Dentistry
} 650 E. 25th Street
} Kansas City, MO 64108-2784
}
} Phone: (816) 235-2072
} Fax: (816) 235-5524
} Web: http://www.umkc.edu/dentistry/microscopy
}
} } -----Original Message-----
} } From: Hans Dijkstra [mailto:hans.dijkstra-at-edax.nl]
} } Sent: Thursday, March 23, 2000 12:19 PM
} } To: DusevichV-at-umkc.edu
} } Cc: Microscopy Listserver
} } Subject: RE: EDS in Variable Pressure SEM
} }
} }
} } Dear Vladimir,
} }
} } The main idea of the ViP-Quant procedure is rather straight-forward and
} } elegant. It is based on the phenomenon that as the pressure increases
the
} } size of the skirt effect remains fairly constant, although the number of
} } skirt electrons increases, and thus the contribution of the skirt-effect
to
} } the EDS signal increases. This intensity increase is linear with the
pressure.
} }
} } This is valid for low- to medium pressures, or at a higher (ESEM)
pressure
} } with a very short free-path length of the skirt electrons, since in both
} } cases you can assume the skirt electrons have scattered only once. At
higher
} } pressures with longer working distances these assumptions are no longer
} } fully valid.
} }
} } So to do an EDS analysis you take 2 measurements at different pressures,
the
} } low-pressure one at a pressure where you can just avoid charging, and a
} } high-pressure one at at least twice that pressure. The measured
intensities
} } of all elements are then plotted as a function of pressure, and
extrapolated
} } to pressure zero, i.e. high vacuum. The extrapolated results will be
very
} } close to the results hat would have been obtained directly if the sample
} } could have been analyzed at high vacuum conditions.
} }
} } Of course anyone can do this plotting and extrapolation manually, that
is:
} } if your EDS software allows you to enter intensities manually! The only
} } thing the EDAX ViP-Quant is offering as an extra is that the software
does
} } it automatically for you. There are no proprietary miracles involved,
EDAX
} } has just listened carefully to what science has to offer....
} }
} } Best regards,
} }
} } Hans Dijkstra
} }
} } Disclaimer: The above is my personal opinion, and not necessarily
EDAX's.
} } -------------------------------------------------------------
} } EDAX Europe www.edax.com
} } Ringbaan Noord 103 Tel.: +31-13-5364000
} } P.O. Box 4144 Fax.: +31-13-5356279
} } 5004 JC Tilburg E-mail: hans.dijkstra-at-edax.nl
} } the Netherlands
} } -------------------------------------------------------------
} }
} } } -----Original Message-----
} } }
} } } From: "Dusevich, Vladimir", INTERNET:DusevichV-at-umkc.edu
} } } To: Everett Ramer, INTERNET:Everett.Ramer-at-netl.doe.gov
} } } "'Hans Dijkstra'", INTERNET:hans.dijkstra-at-edax.nl
} } }
} } } CC: Joergen Bilde-Soerensen 5802, INTERNET:j.bilde-at-risoe.dk
} } } [unknown], INTERNET:Microscopy-at-sparc5.microscopy.com
} } }
} } } Date: 3/22/00 4:35 PM
} } }
} } } RE: RE: EDS in Variable Pressure SEM
} } }
} } }
} } }
} } --------------------------------------------------------------
} } ----------
} } } The Microscopy ListServer -- Sponsor: The Microscopy
} } Society of America
} } } To Subscribe/Unsubscribe -- Send Email to
} } ListServer-at-MSA.Microscopy.Com
} } } On-Line Help
} } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } }
} } --------------------------------------------------------------
} } ---------.
} } }
} } }
} } } Hans,
} } } Could you please explain the main idea of the
} } } Vip-Quant algorithm. I cannot even imagine
} } } how quantitative analysis could have nearly the
} } } same accuracy as in high-vacuum mode without
} } } a priori knowledge of the composition of
} } } surrounding areas.
} } } Thank you,
} } }
} } } Vladimir
} } }
} } }
} } } Vladimir M. Dusevich, Ph.D.
} } } Electron Microscope Lab Manager
} } } 3127 School of Dentistry
} } } 650 E. 25th Street
} } } Kansas City, MO 64108-2784
} } }
} } } Phone: (816) 235-2072
} } } Fax: (816) 235-5524
} } } Web: http://www.umkc.edu/dentistry/microscopy
} } }
} } } } -----Original Message-----
} } } } From: Hans Dijkstra [mailto:hans.dijkstra-at-edax.nl]
} } } } Sent: Tuesday, March 21, 2000 7:54 AM
} } } } To: Everett Ramer
} } } } Cc: Microscopy-at-sparc5.microscopy.com; Joergen Bilde-Soerensen 5802
} } } } Subject: RE: EDS in Variable Pressure SEM
} } } }
} } } }
} } } } Dear Everett,
} } } }
} } } } The extrapolation method (aka Variable Pressure Method)described by
Dr.
} } } } Bilde-Sorenson has been implemented by EDAX in a software feature
called
} } } } ViP-Quant. With this method the EDS analysis under Bad-Vacuum
conditions can
} } } } be done with nearly the same accuracy as under High-Vacuum
conditions.
} } } } Although this method was developed with the ESEM microscope in mind,
it of
} } } } course can be applied to all Bad-Vacuum scanning electron
microscopes.
} } } }
} } } } Please contact your local EDAX representative for more information
and a
} } } } copy of the new ViP-Quant brochure, or request one through
www.edax.com.
} } } }
} } } } With best regards,
} } } }
} } } } Hans Dijkstra



From daemon Fri Mar 24 08:48:26 2000



From: earlw-at-pacbell.net
Date: Fri, 24 Mar 2000 06:38:04 -0800
Subject: Re: Dumping of Service Contracts costs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Allen,

Thanks for the info. I really didn't want to go through & look up the FAR
again.

The GSA wasn't my real inquiry anyway. I just wanted to find out how widespread
this practice extends. The GSA issue seems to get in the way.

OK to rephase: How many have experienced an extreme reduction in service
contract price (20% or more) when confronted with competition?

Thank You,

Earl Weltmer


"Allen R. Sampson" wrote:

} Actually, both are right here. Government stipulations have apparently
} eased recently, but a part of the government contract procedure has in the
} past been a certification by the service provider that the rates being
} quoted are normal and appropriate. This has essentially required details
} of a few other comparable contracts that were similar in price and
} requirements.
}
} I have not had to go through that particular body cavity search for some
} time. I assume the government learned that certifications of this sort are
} basically worthless, as there are companies large and small who are willing
} to do a little spin control in order to secure contracts. Government
} agencies are probably unlikely to bother verifying compliance with their
} regulations - not a problem in this field alone.
}
} My memory goes only so far, as does my desire to look up old records.
} However, if you're really interested, you may want to start with the old
} DAR (Defense Acquisition Regulation) 7-1903.41 - Service Contract Act of
} 1965, also updated and known as FAR 52.222-40. In the late 80's, it seems
} to have been a part of the small business set-aside program (FAR 52.219-04)
} to require a price list or a verifiable listing of 3 similar items sold to
} establish a market price. You might also look into the Walsh-Healey Public
} Contract Act, FAR 52.222-20.
}
} DOD FAR Supplement (46 CFR Chapter 2) clause 11.111-10 states - "...The
} Contractor agrees that the prices for the supplies or services furnished
} under this contract are as low or lower than those charged the supplier's
} most favored customer for comparable quantities under similar terms and
} conditions, in addition to any discounts for prompt payment."
}
} Frankly each government contract I have generates about 1/2" of paper work
} per year. I give little concern to pricing matters, either I win a
} contract or not. The same goes for commercial customers. I try to price
} my services to all customers based on the expenses I expect to incur and a
} modest income for myself (perhaps way too modest, I reflect, as the time to
} finish the tax year has come). There are many other regulations and laws
} that I have to pay more attention to.
}
} Whether these, and other, regulations, laws and requirements have any
} actual practical effect is open to debate. I can't say that I have noticed
} any recent trend for manufacturers to discount their service contracts -
} although I don't normally inquire about other bids submitted. I wouldn't
} be surprised, though, if manufacturers are finding it advantageous to
} discount service prices now to capture customers while the capital
} expenditures are running high in the current economy. However, when those
} capital budgets dry out in the next economic cycle, they will find that the
} service end will have a larger effect on their profitability and they will
} increase their profit margins as best they can.
}
} Consider this a normal result of the economic cycles that we are all slaves
} to. As a third party service provider, you can generally think of me as an
} independent car repair shop. In good times, people will go to
} manufacturer's service, or buy a new car - in bad times they will make
} every effort to stretch their capital investment. In good economic times,
} I catch a good deal of lower end business from those who have instruments
} orphaned by their manufacturer or are very price conscious. In bad
} economic times, I'm the guy who can help you avoid laying off personnel by
} reducing your costs.
}
} In either case, just be glad that there are independent service sources
} available for these instruments and consider that they will only be there
} as long as it is economically feasible for them to survive all economic
} scenarios. That is even more true for manufacturers who would rather sell
} you a new instrument than service a 3 year old instrument. That
} short-sighted corporate position has proven to be a death knell in this
} industry. In many cases it has only been the availability of third party
} service that has allowed a company to survive in a given market for a
} little longer. Public relations is a two-sided blade - it can cut real
} deep when times are bad.
}
} Government laws, rules and regulations can have a significant effect on
} manufacturers serving both government and commercial customers. As has
} been stated before, manufacturers wishing to do business with the
} government are often required to provide service for a stated period of
} time and, as stated here, are required to provide service at a market rate.
} A first year law student would recognize that, in this country, commercial
} concerns benefit from the standards set by the government. It would
} behoove any company to provide an even pricing structure across the board,
} as any other position opens them up to legal action from either side.
}
} Since the government rules require comparison under similar "terms and
} conditions", the question of multiple instrument discounts is appropriately
} accounted for. We are not talking '"apples and oranges" here. Rather, we
} are talking about a closed feedback loop where any change in either side
} must affect the other. The only wiggle-room here is in the quality of
} service provided. Yes, the consumers, both government and commercial, can
} bid the service price down. But in the end, only the service quality will
} be compromised.
}
} Allen R. Sampson, Owner
} Advanced Research Systems, St. Charles, Illinois 60174
} voice 630.513.7093 fax 630.513.7092
}
} -----Original Message-----
} From: Dr. Gary Gaugler [SMTP:gary-at-gaugler.com]
} Sent: Thursday, March 23, 2000 6:18 PM
} To: Earl Weltmer
} Cc: MSA listserver
} Subject: Re: Dumping of Service Contracts costs
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hum.... This is very interesting. Government customers are one
} thing, non-government customers are another. GSA rules and
} principles do not apply to non-government customers. If you
} would please cite the FAR that is the basis for your assertion,
} this could help a lot to clarify your statement.
}
} I have never heard of "price dumping." But I have heard of
} competition. One must keep U.S. government business separate
} from others. Even then, how does one mingle them together?
} On what basis is this done?
}
} How much of this "competition" is based on multiple unit discounts versus
} actual price reduction? And as a consumer, what real difference
} does it make? If the consumer can negotiate a good deal, all
} the better for the consumer, right?
}
} Mixing GSA into free enterprise is like apples and oranges, so
} to speak.
}
} What do you think?
}
} gary g.
}
} At 03:19 PM 3/23/00 , you wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Hi All,
} }
} } I've heard from several SEM/TEM independent maintenance companies that
} } some of the manufacturer's have engaged in "price dumping" when
} } negotiating a service contract.
} }
} } In other words, if the manufacturer is aware that they have competition
} }
} } for a service contract they will reduce the contract by as much as forty
} }
} } percent.
} }
} } I am all for the free enterprise system, but I thought that when a
} } contract price is offered it is the same for all customers especially
} } government contracts that must abide by the GSA rules. GSA rules states
} }
} } that the price offered is the lowest price for this service. It would
} } be
} } illegal for the manufacturer (or anyone) to offer a contract at less
} } than the cost offered to GSA customers.
} }
} } I am surprised by this move as our contracts are the same as we abide
} } by
} } the GSA rules.
} }
} } Regards,
} }
} } Earl Weltmer



From daemon Fri Mar 24 09:01:15 2000



From: Kingsley Micklem :      kingsley.micklem-at-cellular-science.oxford.ac.uk
Date: Fri, 24 Mar 2000 08:50:13 -0600
Subject: LR White embedding

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We find that we get better silver staining of cross-sections of hair
viewed bt TEM when embedded in LR White than Araldite resin.

We are looking for a way to allow us to orientate a bundle of hair fibres
(about 5mm long) and keep the bundle intact whilst heat-curing the LR
White anaerobically. We do not have the means to lower the temperature of
the resin, nor use UV light for curing.

Can anyone suggest a means of keeping the bundle of hairs intact within
the LR White whilst embedding and curing? Any support must be able to
withstand the effects of acetone and LR White resin in liquid form.

Thanks for your attention

Jeremy Sanderson
Please reply to jb_sanderson-at-yahoo.com

**********************************************************************
Dr Kingsley Micklem * MEDICAL INFORMATICS UNIT
Director, Medical Informatics Unit * CELLULAR SCIENCE
University Department of Cellular Science *
Room 5501, John Radcliffe Hospital, * OXFORD
Headington, Oxford, OX3 9DU *
Tel 44 (1865) 222039 * website:
FAX 44 (1865) 221693 *http://phoenix.jr2.ox.ac.uk
**********************************************************************




From daemon Fri Mar 24 09:20:32 2000



From: Marie E. Cantino :      cantino-at-uconnvm.uconn.edu
Date: Fri, 24 Mar 2000 10:18:53 -0500
Subject: TEM, Biological, sinking of tissue in sucrose

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Here is a question for those of you who use sucrose to provide
cryoprotection for tissues:

We are preparing perfusion fixed brain tissue by freeze substitution for
post-embedding immuno gold. Following fixation we prepare 500 micron
vibratome sections, which we then wish to infiltrate with 2 M sucrose in
buffer prior to freezing. We have been transferring the brain sections to
1 M sucrose, then when they sink, transferring again to 2 M. The problem
is that the sections never sink in the 2 M sucrose.

Do others have this problem? Do we have to cut the sections into small
pieces first, or is there some other way to get them to go under? Or
should we not worry about making them sink?

Thanks for your advice.

Marie

Dr. Marie E. Cantino
Dept. of Physiology and Neurobiology, U-2131
University of Connecticut
Storrs, CT 06269-2131
Phone: 860-486-3588
Fax: 860-486-1936




From daemon Fri Mar 24 09:31:30 2000



From: Tom Phillips :      PhillipsT-at-missouri.edu
Date: Fri, 24 Mar 2000 09:28:22 -0600
Subject: Re: LR White embedding

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have done similar things in a manner such as this:
Take the cap off a BEEM capsule and lay it down open side up. Lay
your hair fibers flat in the cap and add LRW. Place a glass slide on
top of the cap to seal it off during polymerization. Heat
polymerize. Use a dremel moto-tool or jewelers hacksaw to trim out a
rectangle of the LRW. Good luck, Tom




}
}
} We find that we get better silver staining of cross-sections of hair
} viewed bt TEM when embedded in LR White than Araldite resin.
}
} We are looking for a way to allow us to orientate a bundle of hair fibres
} (about 5mm long) and keep the bundle intact whilst heat-curing the LR
} White anaerobically. We do not have the means to lower the temperature of
} the resin, nor use UV light for curing.
}
} Can anyone suggest a means of keeping the bundle of hairs intact within
} the LR White whilst embedding and curing? Any support must be able to
} withstand the effects of acetone and LR White resin in liquid form.
}
} Thanks for your attention
}
} Jeremy Sanderson
} Please reply to jb_sanderson-at-yahoo.com
}
} **********************************************************************
} Dr Kingsley Micklem * MEDICAL INFORMATICS UNIT
} Director, Medical Informatics Unit * CELLULAR SCIENCE
} University Department of Cellular Science *
} Room 5501, John Radcliffe Hospital, * OXFORD
} Headington, Oxford, OX3 9DU *
} Tel 44 (1865) 222039 * website:
} FAX 44 (1865) 221693 *http://phoenix.jr2.ox.ac.uk
} **********************************************************************

Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)



From daemon Sat Mar 25 08:58:05 2000



From: Tobias Baskin :      BaskinT-at-missouri.edu
Date: Fri, 24 Mar 2000 09:31:16 -0600
Subject: Re: LR White embedding

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Jeremy,
We have had good luck keeping roots straight by sanwiching
them between films of formvar on wire loops. We make a loop with a
3mm diameter and a long stem out of very thin copper wire (36 gauge
in usa). We then cast rectangles of formvar about 4 mm x 8 mm and
line the loop up over the floating rectangle so that the plane of the
loop is parallel to the short side of the rectangle and right in the
middle. Then we quickly plunge the loop straight down onto the
formvar and into the water and pull it back out again. This gives us
a nice film on the loop. Give this at least a few hours to dry (but
they will keep for ages--we plant the stem of the loop in some wax
and cover with a beaker). Then when ready to go, place your sample,
either before or after fixation, on the loop and repeat with a second
formvar rectangle.Now you have the sample sandwiched. It is very nice
for small samples because solution exchange becomes a snap. The
formvar definitely stands up to actetone, butyl methyl methacrylate,
Spurs, and I am 95% sure LR white. It is easy to dehydrate through
and get other things through even antibodies go through (although
they are slowed down a bit).

Hope this helps.

You got more questions, let me know.

Tobias Baskin


} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_ ____ ^ __ ____ Tobias I. Baskin
/ \ / / \ / \ \ University of Missouri
/ | / / \ \ \ Biological Sciences
/___/ /__ /___ \ \ \__ 109 Tucker Hall
/ / / \ \ \ Columbia, MO 65211-7400 USA
/ / / \ \ \ voice: 573-882-0173
/ /____ / \ \__/ \____ fax: 573-882-0123



From daemon Sat Mar 25 08:58:06 2000



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Fri, 24 Mar 2000 09:39:19 -0600
Subject: RE: LR White embedding

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We are facing a similar problem here and have just had some preliminary
success with polymerizing LR White in a vacuum dessicator placed in an oven.
We were using gelatin capsules full of LR White placed upside down over
coverslips upon which cells have been grown. Standard polymerization did
not adhere the gel caps to the coverslips, supposedly due the presence of
oxygen (it was worth a try). However, the trial run in the dessicator seems
to have worked well, and the cover slip detached cleanly when placed in
liquid nitrogen.

You might try this with flat embedding molds to preserve the orientation of
your bundles. If you do, please let us know the results.

Another approach might be to embed the hair bundles and later cut them out
of the LR White in a piece of resin, orient them the way you want, and glue
the resin piece onto the tip of a blank block for ultramicrotomy.

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/



------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


We find that we get better silver staining of cross-sections of hair
viewed bt TEM when embedded in LR White than Araldite resin.

We are looking for a way to allow us to orientate a bundle of hair fibres
(about 5mm long) and keep the bundle intact whilst heat-curing the LR
White anaerobically. We do not have the means to lower the temperature of
the resin, nor use UV light for curing.

Can anyone suggest a means of keeping the bundle of hairs intact within
the LR White whilst embedding and curing? Any support must be able to
withstand the effects of acetone and LR White resin in liquid form.

Thanks for your attention

Jeremy Sanderson
Please reply to jb_sanderson-at-yahoo.com

**********************************************************************
Dr Kingsley Micklem * MEDICAL INFORMATICS UNIT
Director, Medical Informatics Unit * CELLULAR SCIENCE
University Department of Cellular Science *
Room 5501, John Radcliffe Hospital, * OXFORD
Headington, Oxford, OX3 9DU *
Tel 44 (1865) 222039 * website:
FAX 44 (1865) 221693 *http://phoenix.jr2.ox.ac.uk
**********************************************************************




From daemon Sat Mar 25 08:58:08 2000



From: Shu-You Li :      syli-at-mail.uni-mainz.de
Date: Fri, 24 Mar 2000 18:07:16 +0100
Subject: TEM: Personal TEM website, updated.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


off the top of my head:
pack hairs into a small tube made out of thin paper - velin tissue or
Rizla cigarette paper wrapped round a cocktail stick - the gum on
the edge of a cigarette paper should survive the resin


Date sent: Fri, 24 Mar 2000 08:50:13 -0600
To: Microscopy-at-sparc5.microscopy.com
} From: Kingsley Micklem {kingsley.micklem-at-cellular-science.oxford.ac.uk}


Hi, all,

Many suggestions have been given to me after my posting the Personal TEM Website http://syli.homepage.com in this list sever. Thanks a lot to these contributers!

Now the site has been updated according to these good suggestions. More journals are added into the TEM-related Journals. and an additional page for JOB LIST and RESUMES was included for head-huntings in TEM region. Now you may send me your head-hunting ads or your resume to me and I will place it in this part. Thanks again.

Shu-You Li
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Please visit my new homepage - a personal website on transmission electron microsocpy (TEM) - at http://syli.homepage.com at your convenience!
It contains not only my current research work, but also LINKS to useful journal informations, instruction for authors, on-line EELS database, periodic table, physical constants, online TEM course and etc. It also contains JOB list and RESUMEs for head-hunting in TEM region.
I am looking forward to your invaluable suggestions!
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
**************************************************
Shu-You Li, Dr.
Institut fuer Physikalische Chemie
Johannes Guttenberg Universitaet
Jakob-Welder-Weg 11
D-55099 Mainz, Germany

E-mail: syli-at-mail.uni-mainz.de; syli16-at-hotmail.com
Fax: +49-6131-3923768
Tel: +49-6131-3923148(O)
**************************************************



From daemon Sat Mar 25 08:58:09 2000



From: Tamara Howard :      howard-at-cshl.org
Date: Fri, 24 Mar 2000 12:14:29 -0500 (EST)
Subject: Re: LR White embedding

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


OK - this is extremely low-tech, but it works (if none of the other
suggestions you receive do the trick). Cotton thread! I've made bundles of
fibres by tying them up with fine sewing thread. You can tie several
places along the length of the bundle so it will be held more firmly if
you need to. You can process, dehydrate, and embed. Just make certain that
you have cotton thread - some of the polymer ones might melt in the
solvents or resins.

I've also heard of using fishing monofilament for stuff that doesn't
require nasty solvents - this is probably just the "guy version" of the
thread technique :)

Tamara Howard
CSHL



On Fri, 24 Mar 2000, Kingsley Micklem wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} We find that we get better silver staining of cross-sections of hair
} viewed bt TEM when embedded in LR White than Araldite resin.
}
} We are looking for a way to allow us to orientate a bundle of hair fibres
} (about 5mm long) and keep the bundle intact whilst heat-curing the LR
} White anaerobically. We do not have the means to lower the temperature of
} the resin, nor use UV light for curing.
}
} Can anyone suggest a means of keeping the bundle of hairs intact within
} the LR White whilst embedding and curing? Any support must be able to
} withstand the effects of acetone and LR White resin in liquid form.
}
} Thanks for your attention
}
} Jeremy Sanderson
} Please reply to jb_sanderson-at-yahoo.com
}
} **********************************************************************
} Dr Kingsley Micklem * MEDICAL INFORMATICS UNIT
} Director, Medical Informatics Unit * CELLULAR SCIENCE
} University Department of Cellular Science *
} Room 5501, John Radcliffe Hospital, * OXFORD
} Headington, Oxford, OX3 9DU *
} Tel 44 (1865) 222039 * website:
} FAX 44 (1865) 221693 *http://phoenix.jr2.ox.ac.uk
} **********************************************************************
}
}
}
}



From daemon Sat Mar 25 08:58:12 2000



From: Sobocinski, Gregg :      Gregg.Sobocinski-at-WL.com
Date: Fri, 24 Mar 2000 13:09:13 -0500
Subject: Re: LR White embedding

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Of course you should all consider that cutting or grinding ANY embedding
resin should be undertaken with the knowledge that resin dust can cause
serious health effects. I don't know if any of my allergies or other health
quirks have been 'encouraged' from exposure to resin dust or solvents, but
judging from the discussions on the listserver about some people's
experiences with resins, I recommend that you treat that dust with the same
respect as paraformaldehyde powder or asbestos!

Just thought I'd share that safety concern.

Gregg Sobocinski
Parke Davis Pharmaceutical Research
Ann Arbor, Michigan, USA
Gregg.Sobocinski-at-wl.com


-----Original Message-----
} From: Tom Phillips [mailto:PhillipsT-at-missouri.edu]
Sent: Friday, March 24, 2000 10:28 AM
To: Kingsley Micklem
Cc: Microscopy-at-sparc5.microscopy.com


I have done similar things in a manner such as this:
Take the cap off a BEEM capsule and lay it down open side up. Lay
your hair fibers flat in the cap and add LRW. Place a glass slide on
top of the cap to seal it off during polymerization. Heat
polymerize. Use a dremel moto-tool or jewelers hacksaw to trim out a
rectangle of the LRW. Good luck, Tom




}
}
} We find that we get better silver staining of cross-sections of hair
} viewed bt TEM when embedded in LR White than Araldite resin.
}
} We are looking for a way to allow us to orientate a bundle of hair fibres
} (about 5mm long) and keep the bundle intact whilst heat-curing the LR
} White anaerobically. We do not have the means to lower the temperature of
} the resin, nor use UV light for curing.
}
} Can anyone suggest a means of keeping the bundle of hairs intact within
} the LR White whilst embedding and curing? Any support must be able to
} withstand the effects of acetone and LR White resin in liquid form.
}
} Thanks for your attention
}
} Jeremy Sanderson
} Please reply to jb_sanderson-at-yahoo.com
}
} **********************************************************************
} Dr Kingsley Micklem * MEDICAL INFORMATICS UNIT
} Director, Medical Informatics Unit * CELLULAR SCIENCE
} University Department of Cellular Science *
} Room 5501, John Radcliffe Hospital, * OXFORD
} Headington, Oxford, OX3 9DU *
} Tel 44 (1865) 222039 * website:
} FAX 44 (1865) 221693 *http://phoenix.jr2.ox.ac.uk
} **********************************************************************

Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)



From daemon Sat Mar 25 08:58:14 2000



From: Brian McIntyre :      mcintyre-at-optics.rochester.edu
Date: Fri, 24 Mar 2000 13:39:17 -0500
Subject: Re: Dumping of Service Contracts costs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


hi earl-
i've been considering the whole service contract routine lately since i
have an older sem, a newer fesem, and a vanilla tem under contract. the
sum of my contracts (from the vendors) is about $40K. i have a hard time
justifying this level of expenditure, but have been burned in the past with
replacement part costs under "limited" arrangements. in my role i have to
recover all lab costs, and contracts are a substantial portion of them.

my main vendor (SEMs) has offered me a 5% "discount". this seems a bit
light (to me) because i am limited in terms of my cost recovery mechanisms.
a commercial or industrial lab would seem to have more flexibility than a
shared facility at a small university. currently i'm negotiating with them
to extend this discount. i'm not sure what will happen if i can't keep
these costs under control...perhaps this is an opportunity for a crafty
entrepreneur. at the very least it may be a signal to look for 3-rd party
service, in-house methods, and/or go bare on older equipment.

brian
********************
} OK to rephase: How many have experienced an extreme reduction in service
} contract price (20% or more) when confronted with competition?
}
} Thank You,
}
} Earl Weltmer

----------------------------------------------
Brian McIntyre
Electron Microscopy Lab- River Campus
Univ of Rochester
Rochester, NY 14627
716-275-3058/4875



From daemon Sat Mar 25 08:58:20 2000



From: Mandayam V. Parthasarathy :      mvp2-at-cornell.edu
Date: Fri, 24 Mar 2000 15:22:24 -0500
Subject: Balzers-Union putter coater

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does anyone have for sale, or know of a supplier of Au/Pd target for the
older model Balzers-Union Sputtering Device Type 07120-A, serial number
235? It is an early version of that coater series and uses targets for an
SCD010 type coater with serial numbers 101-317. The target is ~5 cm
diameter with a threaded mounting hole. Thanks!


*******************************************************************

M.V. Parthasarathy
Prof. of Plant Biology, Adjunct Prof. of Biomedical Sciences (Vet), &
Director, Cornell Integrated Microscopy Center (CIMC)
Section of Plant Biology
228 Plant Science Building
Cornell University, Ithaca, NY 14853
E-Mail: mvp2-at-cornell.edu
Plant Biology Office: 268 Emerson; Telephone: 607-255-1734
Plant Biology Fax: 607-255-5407
CIMC Office: C1 054 Vet. Med. Center; Telephone: 607-253-3803
CIMC Office Fax: 607-253-3803
CIMC web site: http://www.cimc.cornell.edu




From daemon Sat Mar 25 08:58:22 2000



From: Sarah Lundberg :      lundberg-at-nevada.edu
Date: Fri, 24 Mar 2000 13:07:55 -0800
Subject: EPMA Standards and QA, Yucca Mountain

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Listers,
We are trying to do some electron microprobe work on the Yucca Mountain
Project for the DOE. However, we may need external verification of our
standards by a laboratory that is on the Qualified Supplier List,
specifically for the YMP. Therefore, unless a lab has done work
directly for Yucca Mountain specifically, they don't count (even DOE run

national labs). This is all according to our QA people, and I am hoping

that someone out there may have direct knowledge of the YMP QA and/or
know someone who does. Please respond offline to me. Many thanks in
advance,
Sarah

************************************************************************

Sarah A.W. Lundberg Lab
(EPMA) (702) 895-2660 or
Electron Microanalysis and
(SEM) (702) 895-2462
Imaging Laboratory
Office (702) 895-1134
Department of Geoscience, UNLV
Fax (702) 895-4064
4505 S. Maryland Parkway Box 454010
Las Vegas, NV 89154-4010 Dept
Office (702) 895-3262

lundberg-at-nevada.edu
http://www.unlv.edu/Colleges/Sciences/Geoscience/EMP.htm

Home:
4489 De Forest St.
Las Vegas, NV 89103
(702) 871-9635
************************************************************************





From daemon Sat Mar 25 08:58:32 2000



From: David Knecht :      knecht-at-uconnvm.uconn.edu
Date: Fri, 24 Mar 2000 18:56:07 -0500
Subject: Laser for laser tweezer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am thinking of trying to put together a laser tweezer setup on my
microscope. It doesn't seem too difficult for a simple system, but I
am having trouble finding the appropriate laser so I don't have to
build it myself from the module. Has anyone out there found a good
source for a near IR laser appropriate for this application? Any
other words of wisdom for a laser/laser tweezer novice appreciated.
Dave Knecht
--

************************************************************

Dr. David Knecht
Department of Molecular and Cell Biology
University of Connecticut
75 N. Eagleville Rd. U-125
Storrs, CT 06269
Knecht-at-uconnvm.uconn.edu
860-486-2200 860-486-4331 (fax)
home page: http://www.sp.uconn.edu/~mcbstaff/knecht/knecht.html
************************************************************



From daemon Sat Mar 25 08:58:35 2000



From: phil.swab-at-depsci.com (Phil Swab)
Date: Fri, 24 Mar 2000 16:36:36 -0800
Subject: LR White embedding

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Micklem,
I embed a variety of fibers for cross-sectioning using the following.
Collect little round pieces of paper from your office paper hole punch.
Using a sharp needle punch an appropriately sized hole in the center of
several of them. Thread your fibers to be embedded through the center hole
of the paper circles. The number required will depend on the length,
diameter and mass of the fibers to be sectioned. I find that a fine pair
of tweezers and a disectin microscope make this job easier. Gently place
this assembly into your embedding capsule (I use BEEM 00). With a pipette,
slowly add resin down the sides to fill the capsule (I use Spurrs with
Z-6040 adhesion promoter). The fiber/paper assembly will self-center in
the capsule and maintain this orientation through curing. Be careful with
your choice of paper, it must be dry, and some porous paper will outgas
bubbles. Test paper samples ahead of time to identify a suitable type.
I'm sure you can adapt this to work with LR White.

Good luck,


Phil Swab
Engineering Development
Deposition Sciences Inc.
Santa Rosa, CA
707-566-3718
phil.swab-at-depsci.com

-----Original Message-----
} From: Kingsley Micklem
[SMTP:kingsley.micklem-at-cellular-science.oxford.ac.uk]
Sent: Friday, March 24, 2000 6:50 AM
To: Microscopy-at-sparc5.microscopy.com


We find that we get better silver staining of cross-sections of hair
viewed bt TEM when embedded in LR White than Araldite resin.

We are looking for a way to allow us to orientate a bundle of hair fibres
(about 5mm long) and keep the bundle intact whilst heat-curing the LR
White anaerobically. We do not have the means to lower the temperature of
the resin, nor use UV light for curing.

Can anyone suggest a means of keeping the bundle of hairs intact within
the LR White whilst embedding and curing? Any support must be able to
withstand the effects of acetone and LR White resin in liquid form.

Thanks for your attention

Jeremy Sanderson
Please reply to jb_sanderson-at-yahoo.com

**********************************************************************
Dr Kingsley Micklem * MEDICAL INFORMATICS UNIT
Director, Medical Informatics Unit * CELLULAR SCIENCE
University Department of Cellular Science *
Room 5501, John Radcliffe Hospital, * OXFORD
Headington, Oxford, OX3 9DU *
Tel 44 (1865) 222039 * website:
FAX 44 (1865) 221693 *http://phoenix.jr2.ox.ac.uk
**********************************************************************






From daemon Sat Mar 25 08:58:45 2000



From: Pbgrover-at-aol.com
Date: Fri, 24 Mar 2000 22:53:45 EST
Subject: SEM: bacteria on cotton thread

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Esteemed Colleagues,

A client asked me to photograph bacilli and endospores on a cotton thread (he
supplied the sample already sputtered with au/pd). I took one really nice
photo, then began experiencing movement of the thread fibers due to
electrostatic charges. It seems the cells & endospores are concentrated at
the unsupported ends of the fibers; when I look at thte interior, bundled
fibers, which are better restrained, they are practically devoid of cells. I
can only guess that the cells/spores were drawn to the outer edges of the
thread as the buffer evaporated.

Any advice, insights or ideas will be rewarded with a big Thank You, Thank
You, Thank You.

Cheers,

Paul Grover :o)
Chief Microscopist and Bottle Washer
Microvista Laboratory
1220 Cincinnati St.
Lafayette, IN 47904




From daemon Sat Mar 25 08:58:51 2000



From: Gordon Couger :      gcouger-at-rfdata.net
Date: Fri, 24 Mar 2000 23:56:32 -0600
Subject: Re: USE CAUTION if using Dremel moto-tool or hacksaw on resin blocks

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} From: "Sobocinski, Gregg" {Gregg.Sobocinski-at-WL.com}
}
}
} Of course you should all consider that cutting or grinding ANY embedding
} resin should be undertaken with the knowledge that resin dust can cause
} serious health effects. I don't know if any of my allergies or other
health
} quirks have been 'encouraged' from exposure to resin dust or solvents, but
} judging from the discussions on the listserver about some people's
} experiences with resins, I recommend that you treat that dust with the
same
} respect as paraformaldehyde powder or asbestos!
}
} Just thought I'd share that safety concern.

The unrecalled products cause more reaction problems than the
cured product.

But as one that has been too careless with air quality don't risk
you health breathing dusts, chemicals or solvents any more than
absolutely necessary.

I think the reaction products of an automobile my be worse than
most things in the lab so you may need to wear you respirator on
your drive to work. About the time the catalytic converter came
along my asthma really flared. some how I don't thing H2SO3 is
good for me.

Take good care of you health if the average age at death continues
it present course you may have to live to 120 or more.

Take care
Gordon

G. C,. Couger
624 Cheyenne
Stillwater, OK 74075-1411
405 624 2855 between 3:00 pm to 1:00 am CST
405 742 2758 cell




From daemon Sat Mar 25 08:59:04 2000



From: greg erdos :      gwe-at-biotech.ufl.edu
Date: Sat, 25 Mar 2000 07:52:40 -0800
Subject: Re: USE CAUTION if using Dremel moto-tool or hacksaw on resin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We solved the resin dust/ Dremel tool problem by rigging a small vacuum
cleaner with the intake very close to where we would be grinding, which was
usually under a disecting scope. I got the idea while watching a cast
being cut from a broken foot. The cast cutter had a vacuum attached.
Our rig takes care of the fine particles, while larger ones generally fall
to the earth and can be cleaned up later.
This more of an issue with epoxies, I suspect. LR White is a dental resin,
I believe, and the dentist will grind in your mouth or over you using your
chest as a table. I don't know if that means it is safe.

Greg Erdos
University of Florida
Greg Erdos
5410 SE 185th AVe
Micanopy, FL 32667
352-466-0843



From daemon Sat Mar 25 08:59:05 2000



From: Keith Ryan :      KPR-at-wpo.nerc.ac.uk
Date: Sat, 25 Mar 2000 12:57:13 +0000
Subject: Re: Balzers-Union putter coater

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


You might be able to buy a metal foil of a suitable size and fix it to
what you already have (or a custom made holder) using silver-loaded
epoxy?

Goodfellow Metals (England) used to have such foils. I could check
this further if you wish, plus send you their contact details when I
am back in the lab.

Regards - Keith


_______________________________
Dr. Keith Ryan
Marine Biological Association of the UK
The Laboratory
Citadel Hill
Plymouth
Devon PL1 2PB
England

Tel. ++44 (0)1752 633249
Tel. ++44 (0)1752 633279
The 279 number has an answering machine

Fax ++44 (0)1752 633102

e-mail: kpr-at-wpo.nerc.ac.uk



From daemon Sun Mar 26 11:26:58 2000



From: RamaSubrahmanyam K :      ramu8sp-at-ccs.iitb.ernet.in
Date: Sat, 25 Mar 2000 14:01:44 -0600
Subject: Required help to etchquartz

Contents Retrieved from Microscopy Listserver Archives
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Hi,
I had done an evapouration of cobalt on a quartz sample. Now I want to
etch the quartz sample to remove the substrate so that I can do
Transmission Microscopy for charactrization. My problem is that If I etch
the substrate with HF will it be harmful to carbon? If it is so what are
the other materials that can be used to etch quartz.
I will be very pleased if you can answer me.
Thanking you
Ramu,
Senior student in M.Sc,
Dept of Physics,
IIT Bombay.

Adress:
Rama Subrahmanyam .K
H:7, R:227,
IITB, Powai,
Mumbai- 400076.
ph:5781049.

meet me at ramu8sp-at-ccs.iitb.ernet.in
suvee-at-wowmail.com,
srisuvee-at-yahoomail.com
*******************************************************************************




From daemon Sun Mar 26 11:27:14 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Sat, 25 Mar 2000 19:05:16 -0600
Subject: Vacuum containers?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does anyone know of some sort of vacuum vessel that
would hold a good number of boxed specimens under
vacuum? I have several mechanical pumps (dual stage)
but have not seen any sizable, sturdy containers. I would
like to store my specimens under a vacuum at all times
unless loading into the SEM. The purpose is to keep any
fungi, moisture and dust from contaminating the specimens.

Vendor responses are welcome.

gary g.



From daemon Sun Mar 26 11:27:27 2000



From: jim :      jim-at-proscitech.com.au
Date: Sun, 26 Mar 2000 21:53:47 +1000
Subject: RE: Vacuum containers?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The problem with large vacuum containers is strength and therefore cost. Its
those 14.7 pounds for every sq inch the atmosphere exerts on vacuum vessels, a
fact EMists know and understand. Some don't realize that another order of
magnitude in high vacuum makes no real difference to that pressure and a very
poor vacuum still exerts over 14 lbs. on every sq inch of surface area.

The practical and economic solution is the use airtight cabinets with drying
agents or a trickle of dry nitrogen gas. Afterall its mostly moisture that
impedes pumping in the SEM or causes specimens and coatings to fall apart with
time.
Disclaimer: Proscitech ships world-wide a large of desiccating cabinets and
vacuum desiccators. See Online } Contents } Page E7
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Sunday, March 26, 2000 11:05 AM, Dr. Gary Gaugler [SMTP:gary-at-gaugler.com]
wrote:}
} Does anyone know of some sort of vacuum vessel that
} would hold a good number of boxed specimens under
} vacuum? I have several mechanical pumps (dual stage)
} but have not seen any sizable, sturdy containers. I would
} like to store my specimens under a vacuum at all times
} unless loading into the SEM. The purpose is to keep any
} fungi, moisture and dust from contaminating the specimens.
}
} Vendor responses are welcome.
}
} gary g.
}



From daemon Sun Mar 26 11:27:27 2000



From: Ric Felten :      smartech-at-javanet.com
Date: Sun, 26 Mar 2000 08:42:36 -0500
Subject: SEM, Does Any Know the Expected Resolution of a "JEOL, JSM 6400" at High kV

Contents Retrieved from Microscopy Listserver Archives
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I am trying to help a new SEM user w/ a tough resolution shot. For some
reason he was not able to find the quoted resolution for the SEM (JEOL JSM
6400) he was using. If anybody out there happens to know its exact or
approximate quoted resolution, that would be very helpful. His electron
source is W.

Thanks

Ric



From daemon Sun Mar 26 16:23:18 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Sun, 26 Mar 2000 09:58:37 -0600
Subject: RE: Vacuum containers?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


thanks to the many responders about this post. Let me add some
more info to help zero in on a solution--if there is one.

The reason I am seeking a vacuum container is two-fold. First,
I have very limited N2 availability. I use industrial grade bottled N2
and dry it with a molecular sieve for venting my SEM chamber
during specimen exchange. I vent at about 5psi. The bottles
are at 1900 psi and hold 130cu ft of N2. One bottle lasts about
two months. I have two bottles--on-line and standby. A "trickle"
of N2 through a dessicator would work but for how long per
bottle? I don't know.

The other factor for a vacuum container is that if a specimen is
under a roughing pump vacuum, if it is at all defective, the body
of the specimen will implode. Thus saving the time of fooling with
it in the SEM. If it does not implode, then it will be dry and not
require very much pump down time before opening the column
isolation valve. Thus ensuring minimal effect on the column ion
pump.

Steve D'Angelo showed a very nice metal dessicator unit, which
would be great if I had a lot of N2....I presume a lot of N2. If
I take one of my 130 cu ft cylinders and leak N2 into the dessicator,
about how long will it last? And what is the minimum leak pressure
needed to make the dessicator function as a drying unit? I could
get another cylinder of N2 and another sieve for this unit if the
gas volume was sufficient to run the box for two to three months.

Appreciate your feedback.

gary g.




From daemon Mon Mar 27 07:34:21 2000



From: Lucille A. Giannuzzi :      lag-at-mail.ucf.edu
Date: Sun, 26 Mar 2000 23:25:09 -0500
Subject: position needed immediately - slight revision

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


ADVERTISEMENT

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characterization or related areas. The person will be responsible for
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maintenance, equipment installation, operation and maintenance, and be an
investigator in contracted research. The person must be able to conduct
independent research and development in materials characterization with an
emphasis in ion beam technology and have the ability to interact with
students and provide instruction in the use and interpretation of data.
The individual must have extensive knowledge and experience in the
operation and maintenance of materials characterization equipment. AMPAC
is particularly interested in individuals desiring an academic setting to
further their professional goals. The applications will be reviewed
beginning April 17, 2000 and will continue to be reviewed until the
position is filled.

Applicants should send vitae and three references to Dr. Vimal Desai,
Director, 12424 Research Parkway, Suite 408, Orlando, FL 32826. UCF is an
equal opportunity/affirmative action employer. As an agency of the State
of Florida, all application materials and selection procedures are
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*******************************************************************
Lucille A. Giannuzzi, Ph.D.

Associate Professor, Mechanical, Materials, and Aerospace Engineering
Director, UCF/Cirent Materials Characterization Facility
University of Central Florida
12443 Research Parkway, Suite 305
Orlando, FL 32826 email lag-at-mail.ucf.edu phone (407) 275-4354,5,6
fax (407) 275-4321
--------------------------------------------------------------------
"Good judgement comes from experience.

Experience comes from making bad judgement."

Mark Twain
********************************************************************




From daemon Mon Mar 27 07:34:21 2000



From: Ken Tiekotter :      tiekotte-at-up.edu
Date: Sun, 26 Mar 2000 21:09:51 -0800 (PST)
Subject: RE: Vacuum containers?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Gary,
Are you concerned about rmp backstreaming, thereby contaminating your
specimens with hydrocarbon. I assume you are using a filter with a baking
element?
-Ken




From daemon Mon Mar 27 07:34:41 2000



From: Ron Doole :      ron.doole-at-materials.oxford.ac.uk
Date: Mon, 27 Mar 2000 08:24:19 +0100 (BST)
Subject: RE: Vacuum containers?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Gary,
How about a TEM film dessicator? Available from most EM suppliers,
vacuum companies and some scrapped TEMs.

Ron

===========================================================================
Mr. Ron Doole e-mail ron.doole-at-materials.ox.ac.uk
Department of Materials, phone +44 (0) 1865 273701
University of Oxford, fax +44 (0) 1865 283333
Parks Road.
Oxford. OX1 3PH. UK.
============================================================================



From daemon Mon Mar 27 07:34:47 2000



From: Malcolm Haswell :      malcolm.haswell-at-sunderland.ac.uk
Date: Mon, 27 Mar 2000 10:35:33 +0100
Subject: Re: USE CAUTION if using Dremel moto-tool or hacksaw on resin blocks

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greg

you mention using a small vacuum cleaner to clean up resin dust. You
don't specify whether it has an exhaust filter so I think I should just
mention that the typical domestic vacuum cleaner exhausts fine
particulates and may well make the problem worse because it may be
pumping the very size of resin particles out that you want to avoid. We
once used a small portable vacuum cleaner for cleaning up resin dust but
stopped when I realized that there was a potential risk.

I know that there are now domestic and industrial vacuum cleaners which
have very fine exhaust filters and there are also the small
photocopier/toner vacuum cleaners. Has anyone investigated their use for
resin dust?

Malcolm

Malcolm Haswell
Electron Microscopy
School of Sciences
Fleming Building
University of Sunderland
SUNDERLAND SR1 3SD
Tyne and Wear
UK

Tel (0191) 515 2872
e-mail: malcolm.haswell-at-sunderland.ac.uk

greg erdos wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} We solved the resin dust/ Dremel tool problem by rigging a small vacuum
} cleaner with the intake very close to where we would be grinding, which was
} usually under a disecting scope. I got the idea while watching a cast
} being cut from a broken foot. The cast cutter had a vacuum attached.
} Our rig takes care of the fine particles, while larger ones generally fall
} to the earth and can be cleaned up later.
} This more of an issue with epoxies, I suspect. LR White is a dental resin,
} I believe, and the dentist will grind in your mouth or over you using your
} chest as a table. I don't know if that means it is safe.
}
} Greg Erdos
} University of Florida
} Greg Erdos
} 5410 SE 185th AVe
} Micanopy, FL 32667
} 352-466-0843



From daemon Mon Mar 27 07:34:49 2000



From: jim :      jim-at-proscitech.com.au
Date: Mon, 27 Mar 2000 21:16:37 +1000
Subject: RE: Vacuum containers?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In Australia we can readily purchase industrial dry N2, this obviates the
drying agent and filtering of the gas. In WDS, gas proportional spectrometers
are fed with about one bubble a second (holding the outlet into water). At that
rate a cylinder routinely last for over a year and although an expensive gas is
used for that purpose, cylinder rental invariably is the greater cost.
A tight drying cabinet only requires a similar amount of gas to maintain a
positive pressure, however slight. Specimens added to the cabinet should be dry
and I think that a tray of desiccant is a good idea.

No additional cylinder would be required since Gary already has two. I used to
deplete the N2 cylinder after use on the EMs, to nitrogen burst during film
development. Since the EM duty cylinder does not go empty unexpectedly, the
cylinder feeding the drying cabinet can be removed in time obtain a full
cylinder.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Monday, March 27, 2000 1:59 AM, Dr. Gary Gaugler [SMTP:gary-at-gaugler.com]
wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} thanks to the many responders about this post. Let me add some
} more info to help zero in on a solution--if there is one.
}
} The reason I am seeking a vacuum container is two-fold. First,
} I have very limited N2 availability. I use industrial grade bottled N2
} and dry it with a molecular sieve for venting my SEM chamber
} during specimen exchange. I vent at about 5psi. The bottles
} are at 1900 psi and hold 130cu ft of N2. One bottle lasts about
} two months. I have two bottles--on-line and standby. A "trickle"
} of N2 through a dessicator would work but for how long per
} bottle? I don't know.
}
} The other factor for a vacuum container is that if a specimen is
} under a roughing pump vacuum, if it is at all defective, the body
} of the specimen will implode. Thus saving the time of fooling with
} it in the SEM. If it does not implode, then it will be dry and not
} require very much pump down time before opening the column
} isolation valve. Thus ensuring minimal effect on the column ion
} pump.
}
} Steve D'Angelo showed a very nice metal dessicator unit, which
} would be great if I had a lot of N2....I presume a lot of N2. If
} I take one of my 130 cu ft cylinders and leak N2 into the dessicator,
} about how long will it last? And what is the minimum leak pressure
} needed to make the dessicator function as a drying unit? I could
} get another cylinder of N2 and another sieve for this unit if the
} gas volume was sufficient to run the box for two to three months.
}
} Appreciate your feedback.
}
} gary g.
}
}



From daemon Mon Mar 27 07:34:51 2000



From: BARRY SHAW :      Barry.Shaw-at-nottingham.ac.uk
Date: Mon, 27 Mar 2000 13:46:37 GMT0BST
Subject: Microscope Info for Undergrad.s

Contents Retrieved from Microscopy Listserver Archives
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Dear All,

Does anyone no of a good web source for info on light microscopy for
undergraduates. Ideally I'm after reference material on brightfield,
phase contrast and fluorescent microscopies. Some info on new areas
would also be useful. Hope someone out there can help !

Thanks in Advance,

Barry Shaw



Barry Shaw
Senior Imaging Technician
Molecular & Cell Biology
E Floor
School Of Biomedical Sciences
University of Nottingham Medical School
NG7 2UH



From daemon Mon Mar 27 08:07:10 2000



From: Ken Tiekotter :      tiekotte-at-up.edu
Date: Mon, 27 Mar 2000 07:37:35 -0600
Subject: Nomarski DIC

Contents Retrieved from Microscopy Listserver Archives
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Dear All:

I am looking to purchase an Zeiss INKO condenser with 4 Wollaston prisms.
The turret position/Wollaston prism marked IIII was used in conjunction
with the Plan Achromat 6.3x objective.

What was the difference between the INKO condenser (46 52 79) and the INKO
slider III (47 44 44) and INKO slider II (47 44 31)? According to the
literature, the type II INKO slider was for research microscopes and the
type III for the the smaller STANDARD microscope.

Using a Photom I, I have tried the INKO condenser in position IIII with
both type III and type II sliders and am not able to obtain DIC with a
PLAN achromat x6.3 objective.

I am very familiar with the 3 position Wollaston prism condenser and am
able to obtain great interference DIC with position I and the PLAN x16
objective. However, using the 4 position condenser on prism IIII yields
no DIC using the x6.3 objective. As a side note, the prisms are in great
shape and show no sign of separation.

I have noticed the dark fringe interference figures for IIII, III, and II
are oriented at 11:00 and 5:00 when viewed through an inverted condenser,
while in position I, the dark fringe is oriented at 1:00 and 7:00.
(hand held with the condenser inverted, the polarizer is placed on top of
the dove-tailed retainer ring to position and lock the condenser into the
condenser holder, and the INKO slider is held at 45 degrees to the east
-west oriented polarizer under the top condenser lens element, i.e., the
entire system is inverted.) If this makes sense, great!

Any suggestions as to why the number IIII position does not yield DIC
using the 6.3 objective?

Thanks
Ken
---------------
Ken Tiekotter, Adjunct Professor
The University of Portland
Dept. of Biology
5000 N Willamette Bldv.
Portland, OR 97303





From daemon Mon Mar 27 08:07:10 2000



From: MGMANDERS-at-aol.com
Date: Mon, 27 Mar 2000 07:36:22 -0600
Subject: NEED TO PURCHASS: MICRO-HARDNESS TEST

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I NEED TO BUY A MICRO-HARDNESS TESTER (USED) WHICH READS OUT IN ROCKWELL OF
COURSE, IN GOOD WORKING CONDITION.

PLEASE SEND INFO TO mgmaders-at-aol.com

Please help need find one to purchass

Michael G. Manders
EM Technology Inc.
(920)262-8380




From daemon Mon Mar 27 08:07:10 2000



From: MGMANDERS-at-aol.com
Date: Mon, 27 Mar 2000 07:36:44 -0600
Subject: Correction on need to purchass (used) metallurgical hardness

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Need to purchass used Metallurgical hardness tester which reads out in
Rockwell. In good working order which must be able to calbrate.

Please help me to find one to purchass send info to mgmanders-at-aol.com

Michael G. Manders
EM Technology Inc.
(920)262-8380




From daemon Mon Mar 27 08:07:10 2000



From: Ping Li :      pli-at-is.dal.ca
Date: Mon, 27 Mar 2000 09:38:08 -0400
Subject: Info on buying carbon rods

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi, I am looking for carbon rods with spectrographic quality for Edwards
vacuum evaporator. The rods should be about 1.5mm in diameter and 30mm
in length. I have tried several suppliers without success. If any of you
have information regarding who supplies this kind of carbon rods could
you please let me know? Your help will be greatly appreciated. Thank
you.

Ping




From daemon Mon Mar 27 17:24:06 2000



From: Andrea Weisberg :      AWeisberg-at-niaid.nih.gov
Date: Mon, 27 Mar 2000 11:55:58 -0500
Subject: FW: Chesapeake Society for Microscopy -Photoshop Workshop

Contents Retrieved from Microscopy Listserver Archives
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} Chesapeake Society for Microscopy
}
} Is pleased to announce
}
} The PhotoShop Workshop
}
} April 11th,2000
}
} Morning Session
} 9:00am-12: 00 noon
} Introduction and Intermediate level
}
} Afternoon Session
} 1:00pm- 4:00pm
} More Advanced Issues and Discussion
}
} Location: NIH, Building 4 Room 433
}
}
} Seating is limited to 50 people
} Preregistration for CSM members by March 31st –No Charge
} After March 31st –Open Registration
} CSM members –No charge
} Non-members - $20.00 per session
}
} To register Contact Andrea Weisberg
} Phone: (301) 435-1977
} e-mail: aweisberg-at-nih.gov
}
}
} Please register early, we can only seat 50 people.
} Ask me about how to become a CSM member
} Membership $10.00 per year
}
} Andrea S. Weisberg
} NIH/NIAID/LVD
} Bldg. 4/Room 210
} 4 Center Drive
} Bethesda, MD 20892-0445
} phone: (301) 435-1977
} fax: (301) 480-1147
} e-mail: aweisberg-at-nih.gov
}



From daemon Mon Mar 27 17:24:06 2000



From: Earl Weltmer :      earlw-at-pacbell.net
Date: Mon, 27 Mar 2000 08:38:13 -0800
Subject: Re: Dumping of Service Contracts costs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I would be very concerned about vacuum pump oil contamination of your
specimens if you us oil sealed roughing pumps on such a vacuum chamber. Oil
backstreaming will occur unless you use a trap and are meticulous about trap
maintenance.

Ronald Vane
XEI Scientific

-----Original Message-----
} From: Dr. Gary Gaugler {gary-at-gaugler.com}
To: MSA listserver {Microscopy-at-sparc5.microscopy.com}


Hi Brian,

Service contract can be likened to "insurance policies'. We are in the service
business & offer service contracts for less mostly because we can work on
several manufacturers and save on travel time & costs. Still we allot about 20%
of the contract costs to parts.

When you "go bare" (self insure) the equipment will probably be OK for about
two years if the maintenance has been properly done. After that it is anyone's
guess.

When we offer reduced service contract prices for reduced risk (customer buys
the parts or limited number of service calls) the discount is about 20-30% to
cover parts costs. The largest expenditure is labor and parts in that order.

Competition generally helps the equation but the question that I had is when
"competition" is only done when faced with a start-up entrepreneurs.
I would ask for a further discount of at least 20%.

Regards,

Earl Weltmer
(714) 573-9158

Brian McIntyre wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} hi earl-
} i've been considering the whole service contract routine lately since i
} have an older sem, a newer fesem, and a vanilla tem under contract. the
} sum of my contracts (from the vendors) is about $40K. i have a hard time
} justifying this level of expenditure, but have been burned in the past with
} replacement part costs under "limited" arrangements. in my role i have to
} recover all lab costs, and contracts are a substantial portion of them.
}
} my main vendor (SEMs) has offered me a 5% "discount". this seems a bit
} light (to me) because i am limited in terms of my cost recovery mechanisms.
} a commercial or industrial lab would seem to have more flexibility than a
} shared facility at a small university. currently i'm negotiating with them
} to extend this discount. i'm not sure what will happen if i can't keep
} these costs under control...perhaps this is an opportunity for a crafty
} entrepreneur. at the very least it may be a signal to look for 3-rd party
} service, in-house methods, and/or go bare on older equipment.
}
} brian
} ********************
} } OK to rephase: How many have experienced an extreme reduction in service
} } contract price (20% or more) when confronted with competition?
} }
} } Thank You,
} }
} } Earl Weltmer
}
} ----------------------------------------------
} Brian McIntyre
} Electron Microscopy Lab- River Campus
} Univ of Rochester
} Rochester, NY 14627
} 716-275-3058/4875



From daemon Mon Mar 27 17:24:13 2000



From: DKITTLESON-at-PILLSBURY.COM
Date: Mon, 27 Mar 2000 15:35:05 -0500
Subject: Cambridge S240 SEM Available in Mid- April

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html





A Cambridge S240 scanning electron microscope is available to any interested
party for moving expenses and best offer. Please contact me directly.
Thank you and kind regards,
Diana

Diana L. Kittleson
Pillsbury Technology East
737 Pelham Blvd.
St. Paul, MN 55114
651-917-5859
651-917-5850 fax
www.tpclabs.com





______________________________________________________________________
This e-mail and any attachment contains information which is private and
confidential and is intended for the addressee only. If you are not an
addressee, you are not authorized to read, copy or use the e-mail or any
attachment. If you have received this e-mail in error, please notify the sender
by return e-mail and then destroy it.




From daemon Mon Mar 27 17:34:27 2000



From: D. W. McNeil :      dmcneil-at-kwiknet.net
Date: Mon, 27 Mar 2000 17:25:26 -0600
Subject: Snappy :For Gordon Couger

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have used a Snappy digitizer for the last 6 years with good
results. I use it with a Javelin color camera. I,m curious, how did you
get it to work with a monochrome camera?? The Snappy syncs on the color
burst portion of the video waveform. I have never been able to get a
oicture with a monochrome camera or electron microscope.




From daemon Mon Mar 27 17:34:27 2000



From: David Browning :      dbrownng-at-stargate.net
Date: Mon, 27 Mar 2000 17:26:05 -0600
Subject: In Search of PAM Interface Board for Link EDX Pulse Processor

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Sir: I am looking for a PAM interface board/card which would
plug into a slot in a Personal Computer. I have a Link Analytical EDX
connected to a Hitachi S520LB SEM. The pulse processor for the EDX is a
stand alone unit which connects via a 25 pin ribbon cable to a personal
computer of older vintage i.e.. 80286 or 80386 technology. The connection
is into a PAM Interface Card. The person I purchased this unit from was
unable to locate the PAM Interface Board. I am looking for this board.
Does anyone know where I can locate one? Thank You, David Browning
dbrownng-at-stargate.net




From daemon Tue Mar 28 06:41:45 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 27 Mar 2000 18:47:03 -0600
Subject: Hitachi S-4700

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Can anyone tell me what the "street value" might be of this SEM?

Used, unknown condition, but not trashed.

gary g.



From daemon Tue Mar 28 06:41:51 2000



From: phil.swab-at-depsci.com (Phil Swab)
Date: Mon, 27 Mar 2000 22:00:18 -0800
Subject: RE: Vacuum containers?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Gary:
Why not store samples in a standard vacuum desiccator (Fisher Scientific,
VWR, etc). They come in glass (with ground glass or o-ring seals) and
plastic (with o-ring seals). However, don't leave samples in a desiccator
under active pumping. All pumps backstream. It's just a matter of how
much contamination your samples can tolerate. Pump the desiccator to some
nominal reduced pressure (a few seconds is fine) and close the valve. A
silica gel or similar desiccant can be used concurrently in the dessicator
and will more effectively pump residual water out of your samples at this
reduced pressure.

Phil Swab
Engineering Development
Deposition Sciences Inc.
Santa Rosa, CA
707-566-3718
phil.swab-at-depsci.com

-----Original Message-----
} From: Dr. Gary Gaugler [SMTP:gary-at-gaugler.com]
Sent: Sunday, March 26, 2000 7:59 AM
To: MSA listserver
Cc: Steve D'Angelo


thanks to the many responders about this post. Let me add some
more info to help zero in on a solution--if there is one.

The reason I am seeking a vacuum container is two-fold. First,
I have very limited N2 availability. I use industrial grade bottled N2
and dry it with a molecular sieve for venting my SEM chamber
during specimen exchange. I vent at about 5psi. The bottles
are at 1900 psi and hold 130cu ft of N2. One bottle lasts about
two months. I have two bottles--on-line and standby. A "trickle"
of N2 through a dessicator would work but for how long per
bottle? I don't know.

The other factor for a vacuum container is that if a specimen is
under a roughing pump vacuum, if it is at all defective, the body
of the specimen will implode. Thus saving the time of fooling with
it in the SEM. If it does not implode, then it will be dry and not
require very much pump down time before opening the column
isolation valve. Thus ensuring minimal effect on the column ion
pump.

Steve D'Angelo showed a very nice metal dessicator unit, which
would be great if I had a lot of N2....I presume a lot of N2. If
I take one of my 130 cu ft cylinders and leak N2 into the dessicator,
about how long will it last? And what is the minimum leak pressure
needed to make the dessicator function as a drying unit? I could
get another cylinder of N2 and another sieve for this unit if the
gas volume was sufficient to run the box for two to three months.

Appreciate your feedback.

gary g.




From daemon Tue Mar 28 06:41:58 2000



From: Ulrike Zeile :      ulrike.zeile-at-bal-tec.com
Date: Tue, 28 Mar 2000 09:51:05 +0200
Subject: AW: Balzers-Union putter coater

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Mr. Parthasarathy,

Balzers Union renamed to BAL-TEC AG in 1992. But the product line was
maintained and new developments are carried out. There are several coating
systems of this older type in use. So of course we can deliver these targets
furthermore.
Article No. of this target: BU 007 129 -T

Our reprepresentative in USA / Canada:

Techotrade International
7 Perimeter Road
Manchester, NH 03103-3343
Mr. Johnny Hagen
T: +1 603 622-5011

Our representative will contact you directly to assist with further
information.

Ulrike Zeile


BAL-TEC AG
EM-Technology and Application
FL-9496 Balzers
Tel. +423 388 12 36
Fax +423 388 12 60


-----Ursprüngliche Nachricht-----
Von: Mandayam V. Parthasarathy [mailto:mvp2-at-cornell.edu]
Gesendet: Freitag, 24. März 2000 21:22
An: Microscopy-at-sparc5.microscopy.com
Betreff: Balzers-Union putter coater


------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Does anyone have for sale, or know of a supplier of Au/Pd target for the
older model Balzers-Union Sputtering Device Type 07120-A, serial number
235? It is an early version of that coater series and uses targets for an
SCD010 type coater with serial numbers 101-317. The target is ~5 cm
diameter with a threaded mounting hole. Thanks!


*******************************************************************

M.V. Parthasarathy
Prof. of Plant Biology, Adjunct Prof. of Biomedical Sciences (Vet), &
Director, Cornell Integrated Microscopy Center (CIMC)
Section of Plant Biology
228 Plant Science Building
Cornell University, Ithaca, NY 14853
E-Mail: mvp2-at-cornell.edu
Plant Biology Office: 268 Emerson; Telephone: 607-255-1734
Plant Biology Fax: 607-255-5407
CIMC Office: C1 054 Vet. Med. Center; Telephone: 607-253-3803
CIMC Office Fax: 607-253-3803
CIMC web site: http://www.cimc.cornell.edu




From daemon Tue Mar 28 06:41:59 2000



From: Ulrike Zeile :      ulrike.zeile-at-bal-tec.com
Date: Tue, 28 Mar 2000 10:04:40 +0200
Subject: WG: Balzers-Union putter coater

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Dear Mr. Parthasarathy,

Balzers Union renamed to BAL-TEC AG in 1992. But the product
line was maintained and new developments are carried out.
For additional information have a look at our webside www.bal-tec.com.
There are several coating systems of this older type in use.
So of course we can deliver these targets furthermore.
Article No. of this target: BU 007 129 -T

Our reprepresentative in USA / Canada:

Techotrade International
7 Perimeter Road
Manchester, NH 03103-3343
Mr. Johnny Hagen
T: +1 603 622-5011

Our representative will contact you directly to assist with
further information.

Ulrike Zeile


BAL-TEC AG
EM-Technology and Application
FL-9496 Balzers
Tel. +423 388 12 36
Fax +423 388 12 60


-----Ursprüngliche Nachricht-----
Von: Mandayam V. Parthasarathy [mailto:mvp2-at-cornell.edu]
Gesendet: Freitag, 24. März 2000 21:22
An: Microscopy-at-sparc5.microscopy.com
Betreff: Balzers-Union putter coater


-------------------------------------------------------------
-----------

of America
To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
-------------------------------------------------------------
----------.
++
++
++ Does anyone have for sale, or know of a supplier of Au/Pd
++ target for the
++ older model Balzers-Union Sputtering Device Type 07120-A,
++ serial number
++ 235? It is an early version of that coater series and uses
++ targets for an
++ SCD010 type coater with serial numbers 101-317. The target is ~5 cm
++ diameter with a threaded mounting hole. Thanks!
++
++
++ *******************************************************************
++
++ M.V. Parthasarathy
++ Prof. of Plant Biology, Adjunct Prof. of Biomedical Sciences (Vet), &
++ Director, Cornell Integrated Microscopy Center (CIMC)
++ Section of Plant Biology
++ 228 Plant Science Building
++ Cornell University, Ithaca, NY 14853
++ E-Mail: mvp2-at-cornell.edu
++ Plant Biology Office: 268 Emerson; Telephone: 607-255-1734
++ Plant Biology Fax: 607-255-5407
++ CIMC Office: C1 054 Vet. Med. Center; Telephone: 607-253-3803
++ CIMC Office Fax: 607-253-3803
++ CIMC web site: http://www.cimc.cornell.edu
++
++
++



From daemon Tue Mar 28 06:42:14 2000



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Tue, 28 Mar 2000 10:30:00 +0100 (GMT Daylight Time)
Subject: Re: USE CAUTION if using Dremel moto-tool or hacksaw on resin blocks

Contents Retrieved from Microscopy Listserver Archives
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Like Malcolm I gave up on a hand held vacuum cleaner when
sawing resin as I suspected it leaked fine particles. Now
I saw up resin in a large bag in a fume cupboard. The bag
has lasted 5 years. Anyone got a neater/safer method?

Dave



On Mon, 27 Mar 2000 10:35:33 +0100 Malcolm Haswell
{malcolm.haswell-at-sunderland.ac.uk} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Greg
}
} you mention using a small vacuum cleaner to clean up resin dust. You
} don't specify whether it has an exhaust filter so I think I should just
} mention that the typical domestic vacuum cleaner exhausts fine
} particulates and may well make the problem worse because it may be
} pumping the very size of resin particles out that you want to avoid. We
} once used a small portable vacuum cleaner for cleaning up resin dust but
} stopped when I realized that there was a potential risk.
}
} I know that there are now domestic and industrial vacuum cleaners which
} have very fine exhaust filters and there are also the small
} photocopier/toner vacuum cleaners. Has anyone investigated their use for
} resin dust?
}
} Malcolm
}
} Malcolm Haswell
} Electron Microscopy
} School of Sciences
} Fleming Building
} University of Sunderland
} SUNDERLAND SR1 3SD
} Tyne and Wear
} UK
}
} Tel (0191) 515 2872
} e-mail: malcolm.haswell-at-sunderland.ac.uk
}
} greg erdos wrote:
} }
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} } We solved the resin dust/ Dremel tool problem by rigging a small vacuum
} } cleaner with the intake very close to where we would be grinding, which was
} } usually under a disecting scope. I got the idea while watching a cast
} } being cut from a broken foot. The cast cutter had a vacuum attached.
} } Our rig takes care of the fine particles, while larger ones generally fall
} } to the earth and can be cleaned up later.
} } This more of an issue with epoxies, I suspect. LR White is a dental resin,
} } I believe, and the dentist will grind in your mouth or over you using your
} } chest as a table. I don't know if that means it is safe.
} }
} } Greg Erdos
} } University of Florida
} } Greg Erdos
} } 5410 SE 185th AVe
} } Micanopy, FL 32667
} } 352-466-0843
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"



From daemon Tue Mar 28 18:57:09 2000



From: Donald Lovett :      lovett-at-tcnj.edu
Date: Tue, 28 Mar 2000 08:47:09 -0500 (EST)
Subject: Problem with Hummer VII sputter coater

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I have a Hummer VII sputter coater, which has an automatic processing
cycle. As the vacuum drops from 60 millitorr to 40 millitorr (during
which time the high voltage should switch on), the entire process shuts
down. I have tried running the system with the argon valve open and shut.

Any suggestions from users out there?

Thanks,

Don
______________________________________________________________________
Donald L. Lovett e-mail: lovett-at-tcnj.edu
Assoc. Professor, Dept. of Biology voice: (609) 771-2876
P.O. Box 7718 fax: (609) 637-5118
The College of New Jersey
Ewing, NJ 08628-0718





From daemon Tue Mar 28 18:57:13 2000



From: Andy Horsewell :      horsewell-at-ipt.dtu.dk
Date: Tue, 28 Mar 2000 16:26:49 +0200
Subject: Re: Microscope Info for Undergrad.s

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} Does anyone no of a good web source for info on light microscopy for
} undergraduates. Ideally I'm after reference material on brightfield,
} phase contrast and fluorescent microscopies. Some info on new areas
} would also be useful.

Barry,
Try looking at these web sites. The virtual microscopy is fun. You might
want to filter out the stuff of interest to materials and geological
sciences... or leave it in in the name of interdisciplinary microscopy !

Andy

http://micro.magnet.fsu.edu/primer/index.html
http://micro.magnet.fsu.edu/primer/techniques/index.html
http://www.people.virginia.edu/~jaw/mse310l/w4/mse4-1.htm
http://micro.magnet.fsu.edu/primer/virtual/virtual.html
http://spm.aif.ncsu.edu/aif/om.htm




From daemon Tue Mar 28 18:57:13 2000



From: Barbara Foster :      mme-at-map.com
Date: Tue, 28 Mar 2000 10:51:47 -0500
Subject: Re: Microscope Info for Undergrad.s

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Barry,

A number of universities are now using "Optimizing Light Microscopy for
Biological and Clinical Laboratories". It covers all the areas you
mentioned and more. It also includes a number of small experiments which
can be done as a group or on independent study. See our website for
further info.

Caveat: MME does have financial interest in this book.

Best regards,
Barbara Foster
Consortium President
Microscopy/Microscopy Education ...Educating microscopists for greater
productivity.

125 Paridon Street Suite 102 Springfield, MA 01118
PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com
Visit our web site {http://www.MME-Microscopy.com/education}
******************************************************


At 01:46 PM 3/27/00 GMT0BST, BARRY SHAW wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Tue Mar 28 18:57:16 2000



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Tue, 28 Mar 2000 08:41:14 -0800
Subject: Re: Info on buying carbon rods

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Ping,
The last time I went looking for spec-pure carbon rods, our Stores man found
them at: ultra carbon, 900 Harrison St., Bay City, MI48708-8244. They
weren't that exact size, but they are a good place to try.
At 09:38 AM 3/27/00 -0400, you wrote:

}
} Hi, I am looking for carbon rods with spectrographic quality for Edwards
} vacuum evaporator. The rods should be about 1.5mm in diameter and 30mm
} in length. I have tried several suppliers without success. If any of you
} have information regarding who supplies this kind of carbon rods could
} you please let me know? Your help will be greatly appreciated. Thank
} you.
}
} Ping
}
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Tue Mar 28 18:57:16 2000



From: Caroline Schooley :      schooley-at-mcn.org
Date: Tue, 28 Mar 2000 08:54:46 -0800 (PST)
Subject: Re: Microscope Info for Undergrad.s

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} Does anyone no of a good web source for info on light microscopy for
} undergraduates. Ideally I'm after reference material on brightfield,
} phase contrast and fluorescent microscopies. Some info on new areas
} would also be useful. Hope someone out there can help !
}
} Barry -

I can suggest a good CD-ROM: Pagliaro, l., et al., 1997
Microscopy-Tutor. You'll find a description and ordering information in
the Project MICRO bibliography (URL below).

Caroline


Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/PCI/pci.html




From daemon Tue Mar 28 18:57:29 2000



From: William Tivol :      tivol-at-wadsworth.org
Date: Tue, 28 Mar 2000 16:24:48 -0500
Subject: Re: TEM, Biological, sinking of tissue in sucrose

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Marie E. Cantino wrote:

} Here is a question for those of you who use sucrose to provide
} cryoprotection for tissues:
}
} We are preparing perfusion fixed brain tissue by freeze substitution for
} post-embedding immuno gold. Following fixation we prepare 500 micron
} vibratome sections, which we then wish to infiltrate with 2 M sucrose in
} buffer prior to freezing. We have been transferring the brain sections to
} 1 M sucrose, then when they sink, transferring again to 2 M. The problem
} is that the sections never sink in the 2 M sucrose.
}

Dear Marie,
The Handbook of Chemistry and Physics gives a specific gravety
for 2 M sucrose of about 1.25. Is the sp. gr. of your tissue (exclusive of
the exchangable fluids, of course) greater than this? If not, the sections
will never sink. If it is marginally greater, then the sections will sink
when hell freezes over, and you can do cryo easily. If you can determine
the weight of a tissue section of known volume first with fixation fluid,
then after infiltration with 1 M sucrose (and also measure the volume
change), you could extrapolate to the situation for 2 M sucrose (or some-
one with too much time on his/her hands could do this for many sucrose
concentrations). Good luck.
Yours,
Bill Tivol



From daemon Tue Mar 28 18:57:30 2000



From: Karen Kelley :      klv-at-biotech.ufl.edu
Date: Tue, 28 Mar 2000 16:42:34 -0500
Subject: localization of zinc in tissue

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We need to localize zinc in prostate tissue for TEM. Has anyone used
Sulfide-silver method or Zinc-dithizonate method? If so, is there a method
better than the other or new methods that might work on such tissue?

I am also trying to locate a reference by Pihl E. Falkmer S: Trials to
modify the sulfide-silver method for ultrastructural tissue localization of
heavy metals. Acta Histochem 27:34-41, 1967. If you have a copy of this
method please email me.

Thank you


Karen Kelley
Senior Electron Microscopist
UF Biotechnology Electron Microscopy Core Lab
Box 118525 Gainesville Florida
lab:352-392-1184 fax: 352-846-0251
email: klv-at-biotech.ufl.edu



From daemon Tue Mar 28 18:57:31 2000



From: William Tivol :      tivol-at-wadsworth.org
Date: Tue, 28 Mar 2000 17:11:25 -0500
Subject: Re: Required help to etchquartz

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


RamaSubrahmanyam K wrote:

} I had done an evapouration of cobalt on a quartz sample. Now I want to
} etch the quartz sample to remove the substrate so that I can do
} Transmission Microscopy for charactrization. My problem is that If I etch
} the substrate with HF will it be harmful to carbon? If it is so what are
} the other materials that can be used to etch quartz.

Dear Ramu,
Quartz can be etched--slowly--in strong base. A 5-to-10 M
solution of NaOH should do the trick, but if the quartz thickness is in
the mm range, it will take a long time. I don't know whether the base
will be harmful to either carbon or cobalt, but I don't think it will.
Good luck.
Yours,
Bill Tivol



From daemon Tue Mar 28 18:57:32 2000



From: William Tivol :      tivol-at-wadsworth.org
Date: Tue, 28 Mar 2000 17:19:36 -0500
Subject: Re: Info on buying carbon rods

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Ping Li wrote:

} Hi, I am looking for carbon rods with spectrographic quality for Edwards
} vacuum evaporator. The rods should be about 1.5mm in diameter and 30mm
} in length. I have tried several suppliers without success. If any of you
} have information regarding who supplies this kind of carbon rods could
} you please let me know? Your help will be greatly appreciated. Thank
} you.
}

Dear Ping,
I got mine from Ted Pella, but I'm surprized you didn't find
them
in other suppliers' catalogues. Pella lists both carbon and graphite, both

spectroscopically pure and technical grade, in several diameters and
lengths.
I have no connection to Ted Pella, Inc. except that of customer.
Yours,
Bill Tivol



From daemon Tue Mar 28 18:57:35 2000



From: C. Singla :      csingla-at-uvic.ca
Date: Tue, 28 Mar 2000 15:03:00 -0800
Subject: Preparation of tissue culture cells for TEM

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Hello everyone,
I have a student who wants to prepare tissue culture cells for TEM in Agar
blocks. I shall appreciate any suggestions.
Thanks in advance.
C.L.Singla



From daemon Tue Mar 28 21:05:15 2000



From: Keith Moulding :      mcmouldk-at-ust.hk
Date: Wed, 29 Mar 2000 09:16:05 +0800
Subject: Analytical TEMS - The choices

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Greetings,

We are contemplating replacing our analytical TEM. A basic spec we envisage
is STEM, EDS + GIF/PEELS. We are happy to choose the EDS, but we run into
trouble with e-guns, i.e. to go LaB6 or FEG. In particular as we would like
to bolt a GIF/PEELS on to the system.

We have several concerns:
The safety of the FEG gun, considering we are a multi-user facility with
users who have a frequent tendency to bend holders and press the wrong
buttons. How problematic are FEGs, do you have much down time / running
cost due to careless users?

GIF/PEELS: has anybody carried out a systematic comparison between a system
with LaB6 or FEG. Is the extra cost of a FEG (over twice the price) really
justified for GIF/PEELS or will it quite happily run on a LaB6.

The thoughts of fellow microscopy in the same quandary much a appreciated.

Keith.

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Dr. K. Moulding.

Materials Characterisation and Preparation Facility
Hong Kong University of Science and Technology,
Clear Water Bay,
Kowloon,
Hong Kong.

FAX: (852) 2358 2451
TEL: (852) 2358 8724
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~



From daemon Tue Mar 28 21:15:21 2000



From: Paul Webster :      pwebster-at-hei.org
Date: 28 Mar 00 23:59:41 -0800
Subject: Re: TEM, Biological, sinking of tissue in sucrose

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Sucrose infiltration is an essential step in the preparation of cryosections for immunocytochemistry. Aldehyde-fixed biological tissue is infiltrated in 2.0M to 2.3M sucrose to protect the sample from freezing damage when it is subseuently frozen by immersion in liquid nitrogen. If the sample is fixed, the membranes become permiable to sucrose and the sample can be frozen into a vitreous state by immersion in liquid nitrogen. Although it is advised to infiltrate for 24 hr on a rotator, many years of experience has demonstrated that even after 15 min of exposure to sucrose, small pieces of aldehyde-fixed material are sufficiently cryoprotected to allow for successful vitrification. Take no heed of whether the sample has sunk to the bottom of the tube or not, check to see if it has been fixed and has spent sufficient time in the sucrose. If it has, it will be cryoprotected.

Useful reference:
Griffiths, G, McDowall, A, Back, R, Dubochet, J. 1984. On the preparation of cryosections for immunocytochemistry. Ultrastruct. Res. 89:65-78
They showed by quantitative mass measurements that fixed cells are freely permeable to sucrose.

Best regards,

Paul Webster

Paul Webster, Ph.D.
House Ear Institute
2100 West Third St.
Los Angeles, CA 90057

Phone: (213) 273-8026
Fax: (213) 413-6739
e-mail: pwebster-at-hei.org
http://www.hei.org/htm/aemi.htm


Bill Tivoli wrote:
The Handbook of Chemistry and Physics gives a specific gravety
for 2 M sucrose of about 1.25. Is the sp. gr. of your tissue (exclusive of
the exchangable fluids, of course) greater than this? If not, the sections
will never sink. If it is marginally greater, then the sections will sink
when hell freezes over, and you can do cryo easily. If you can determine
the weight of a tissue section of known volume first with fixation fluid,
then after infiltration with 1 M sucrose (and also measure the volume
change), you could extrapolate to the situation for 2 M sucrose (or some-
one with too much time on his/her hands could do this for many sucrose
concentrations). Good luck.

Marie E. Cantino wrote:
We are preparing perfusion fixed brain tissue by freeze substitution for
post-embedding immuno gold. Following fixation we prepare 500 micron
vibratome sections, which we then wish to infiltrate with 2 M sucrose in
buffer prior to freezing. We have been transferring the brain sections to
1 M sucrose, then when they sink, transferring again to 2 M. The problem
is that the sections never sink in the 2 M sucrose.





From daemon Wed Mar 29 07:35:11 2000



From: christine richardson :      a.c.richardson-at-durham.ac.uk
Date: Wed, 29 Mar 2000 09:41:16 +0100
Subject: critical point drying apparatus

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Hi,
Our safety people are concerned about having our Polaron c.p.d
pressure tested at regular intervals.
Does any one out there know of anyone who does this sort of thing,
preferably on site?
Also I would be interested to hear how other users safety check this
sort of equipment.
thanks,
Christine.



From daemon Wed Mar 29 07:35:12 2000



From: Jonathan Barnard :      Jonathan.Barnard-at-Angstrom.UU.SE
Date: Wed, 29 Mar 2000 10:24:56 +0100
Subject: TEM:Analytical TEMs

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Dear Keith,
If you are seriously worried about the experience of some of the users
then you should consider the Philips/FEI Tecnai series of instruments. The
person in charge of the microscope can determine the level of access to all
the features for each user i.e. basic, medium and expert user profiles. The
microcope, in a similar way to fly-by-wire aircraft, will simply prevent
users from blowing the tip up or anything else detrimental to the 'scope.
This should take out the worry about user/FEG problems.

The LaB6 and FEG work identically with the GIF/PEELS, they're only
electrons after all. The FEG will give you a smaller energy width (good for
EELS:ELNES .etc) and more current (good for Energy Filtered TEM,
STEM:HAADF, EELS & EDS).
Then there is the option of adding a biprism with the FEG if you want to
try off-axis holography.
The FEG machine will simply give you a wider tolerance range in terms of
specimen thickness and experimental conditions i.e greater signal to noise
ratios than with the LaB6, something that is a problem with STEM based
methods. FEG alignment procedures will be slightly different too.

We have taken delivery of a 300kV Tecnai and I have to say that the machine
is very impressive. The machine is very stable and the high brightness FEG
is a major improvement over the LaB6. The GIF is also quite a God-send for
zero-loss filtering and for mapping.

I hope this helps, Jon

P.S. I am not affiliated or have any commercial connection to Philips/FEI.
********************************************************
Dr Jonathan Barnard

Analytical Materials Physics
The Angstrom Laboratory, Uppsala University
P O Box 534, SE-751 21 Uppsala, Sweden
Phone: +46-(0)18-4716838 Fax: +46-(0)18-500131
http://www.angstrom.uu.se/analytical/home.html

********************************************************



From daemon Wed Mar 29 07:35:13 2000



From: rajdeep-at-aripune.ernet.in (Rajdeep Dongre)
Date: Tue, 28 Mar 00 18:04:32 PST
Subject: Pirani Gauge of Oxford Hexland - CT1000.

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------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Dear Friends,

The Pirani Gauge of the Oxford Hexland - CT1000 cryo unit mounted on SEM
S120 is not working. Can anyone guide me in purchase of this? Could
this be manufactured in-house? Please help me out.

Rajdeep Dongre
Electron Microscopy Laboratory
Agharkar Research Institute
G.G. Agarkar Road
Pune - 411 004, India
Phone 91-20-5653680/5654357
e-mail : rajdeep-at-aripune.ernet.in



From daemon Wed Mar 29 07:35:17 2000



From: Anne Heller :      heller-at-Uni-Hohenheim.DE
Date: Wed, 29 Mar 2000 14:23:00 +0200
Subject: Epon sections

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Message-ID: {38E1F5A4.96CAA8EC-at-uni-hohenheim.de}


We work with plant material and after ultra thin sectioning our sections
show lines of compressions in cell wall areas. Of course, we try to
stretch the Epon sections with a heat pen or with chloroform, but very
often we are not successful. The sections just move on the water
surface and nothing is happening. Any advice would be gratefully
appreciated.
Regards,
Anne Heller
--
Dr. Anne Heller, AG Elektronenmikroskopie,
Institut fŸr Botanik (210), UniversitŠt Hohenheim,
Garbenstra§e 30
D-70593 Stuttgart

Tel.: (0049)-711-459-2180
Fax.: (0049)-711-459-3355




From daemon Wed Mar 29 17:30:29 2000



From: GuessWho :      olivier.balmes-at-oorg2.lth.se
Date: Wed, 29 Mar 2000 16:26:58 +0200
Subject: Re: EFTEM/GIF on particles

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Hi Jo,
Thanks for your answer.
We use our GIF in a similar way, focusing around the energy loss we want to
take the image at with a slit width covering the 3 energy windows if possible.
This works best for rather thick samples (compared to our 5 nm particles)But
as you say, maybe it's just the spatial resolution which is not good enough,
our microscope was designed to enhance contrast for biological studies,
unfortunately loosing some resolution in the process. We are not yet trying to
get concentrations out of the pictures...rather get a qualitative mapping.

I tried to do EELS measurements a few times and got very few succes. The best
results are obtained on beam resistant samples simply because I can then move
the zero loss peak off the CCD and increase intensity (or acq time) at will. I
start with a very low intensity and optimise it in turboview mode to get a
good signal out. However I could seldom see well defined peaks.
One point which I find strange is that we can often get reasonnably good
elemental maps even when we don't see any peak in the EELS spectrum. The
question is then if the map is really trustworthy...

Any comments welcome !

Olivier



Jo Verbeeck wrote:

} Hello,
}
} Regarding EFTEM on small particles, this should actually work quite well.
} That is, elemental mapping works reasonably if you choose the right
} objective apperture. You should simulate the spatial resolution to see
} what can be attained under your conditions (HT, Cc, Cs etc).
} However it will be very difficult to get real concentration information
} out of these images. I'm trying EELS with nanoprobe but so far no succes
} (everything gets destroyed before I take a spectrum, allignment is very
} very difficult)
} Still, focussing remains a problem. Comon practice is to focus at 100eV
} and then suppose everything is OK for higher losses. I do not understand
} the theory of this technique (blurring by finite slitwidth & Cc?) nor thus
} it work well. Any comments on this are welcome.
}
} Jo
}
} *************************************************************
} * Jo Verbeeck *
} * University of Antwerp *
} * Dept. EMAT (Electron Microscopy for Materials Research) *
} * e-mail: joverbee-at-ruca.ua.ac.be *
} * tel: +32(0)3 218 02 49 *
} * fax: +32(0)3 218 02 57 *
} *************************************************************
}
} On Tue, 21 Mar 2000, GuessWho wrote:
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Dear all,
} }
} } I was wondering if anybody was using a GIF filter. We have one mounted
} } on a 120 kV TEM and are trying to image small particles (5-10 nm) and
} } doing some energy filtering to image different elements in the particles
} } (for example gold, silicon, copper, cerium...) . This is not really what
} } I would call trivial work and we are still working out the set up
} } (window size and positionning for example) in a rather empiric way. The
} } main problem is often to get a decent signal in the interesting energy
} } region and still keep the windows fairly narrow.
} } I would add that we often have to work at low dose because of the beam
} } sensitivity of our samples, but I guess our GIF setups can be improved.
} } Any suggestion ?
} }
} } Any general comment on the GIF warmly welcome !
} }
} } Thanks
} }
} } Olivier
} }
} }
} }



From daemon Wed Mar 29 17:30:30 2000



From: John Heckman :      heckman-at-pilot.msu.edu
Date: Wed, 29 Mar 2000 09:46:56 -0800
Subject: sRe: Epon sections

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Anne Heller wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} We work with plant material and after ultra thin sectioning our sections
} show lines of compressions in cell wall areas. Of course, we try to
} stretch the Epon sections with a heat pen or with chloroform, but very
} often we are not successful. The sections just move on the water
} surface and nothing is happening. Any advice would be gratefully
} appreciated.
} Regards,
} Anne Heller
} --
} Dr. Anne Heller, AG Elektronenmikroskopie,
} Institut fŸr Botanik (210), UniversitŠt Hohenheim,
} Garbenstra§e 30
} D-70593 Stuttgart
}
} Tel.: (0049)-711-459-2180
} Fax.: (0049)-711-459-3355

Hi Anne,

My first guess is that the mechanical properties of your Epon mix differs
too much from that of the fixed walls. However, when I worked with plant
material, I generally used Xylene to stretch floating sections (rarely in
Epon). I'm not sure if was the aromatic character or the boiling point
difference that made it better at swelling and relaxing the sections than
CHCl3.

Other suggestions would be using a harder resin (my favorite was VCD and
Quetol 651 (equiequivalent) hardened with NSA (or HSA), which I called
Spurtol ;-)). I found it stains easier than Spurr's yet is low viscosity
and about as hard as a medium Spurr's. Reducing the knife angle might
help. Post curing the existing blocks at elevated temperature might
harden them some more.

Cheers,

John

John Heckman
MSM Department
Michigan State University



From daemon Wed Mar 29 17:30:30 2000



From: Kenneth JT Livi :      klivi-at-jhu.edu
Date: Wed, 29 Mar 2000 09:58:03 -0400
Subject: Re: TEM:Analytical TEMs

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I don't know if you have interest in this but it is good info.
Wallace

-----Original Message-----
} From: Parker, Jo
Sent: Wednesday, March 29, 2000 8:45 AM
To: All SOD Faculty & Staff


Dear Jonathan and Keith,

I just want to add a few comments to what Keith said about FEG's. One of
the major advantages of a FEG over conventional souces for AEM is the spot
size. The new FEG scopes have STEM resolution of 2 angstroms with great
brightness. (I have only seen the Philips/FEI scope, but I think the other
companies have similar specs.) When one is deciding whether or not to go
for the FEG, I think it will depend on your applications. If a small
intense beam and good energy resolution are necessary, go for the FEG. Of
course, there is also the consideration of the cost of service contract
($$$$).

Ciao for now,
Ken




From daemon Wed Mar 29 17:30:30 2000



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Tue, 28 Mar 2000 10:09:25 -0500
Subject: CPD safety question

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-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Christine Richardson wrote:
===================================================
Hi,
Our safety people are concerned about having our Polaron c.p.d
pressure tested at regular intervals.
Does any one out there know of anyone who does this sort of thing,
preferably on site?
Also I would be interested to hear how other users safety check this
sort of equipment.
===================================================
I might not be the last word on this, but I had described to me the kind of
large liquid filled tank (I think it is water) that pressure vessels like
this are tested in, and to me at least it did not sound like it would be the
kind of thing that one would "carry around" in a portable kind of unit to do
this kind of testing, on site. This description came, some years ago, from
Dr. Wilf Gee (now deceased) and who was very much involved in the original
manufacturing of this product and who had involvement for many years with
the safety testing of the unit.

When your CPD unit was first delivered, it would have come with a copy of a
report from the testing agency showing the results and conditions of the
pressure testing. I might be off by a bit on this, but it was my
recollection that the vessel is tested to a pressure level that is three or
four times higher than what would be required to blow out the rupture disc.
In other words the unit is tested to a level that, if the rupture disc did
not blow exactly where it was supposed to blow, there is some very large
margin of error so that the disc still would blow long before the vessel.

There are probably more CPD units of this design installed in the world than
any other design. I have never heard of anyone having any kind of a problem
with it (e.g. an explosion), except an occasional blowing of a rupture disc,
but certainly not the pressure vessel itself. Just don't ever try to run
the unit by replacing the rupture disc, if one is not handy, with a cut
metal disc. That is very dangerous and should never be done. Believe it or
not, we do uncover once in a while, people who do do that sort of thing, out
of naivety of course and that is why I do not waste an opportunity to point
out the danger in doing that sort of thing. That is why I always have
recommended, for multi-user environments in particular, to always have a
small supply of replacement rupture discs near by the unit just to avoid
even the slightest temptation (such as by a student) to by-pass this
important safety feature.

So I for one would be very interested in hearing the logic and rationale of
your safety committee that is of the opinion that you should have your
pressure vessel tested from time to time.

However, there is one kind of situation where one should have the vessel
pressure tested and that is if one has attempted any mechanical
modifications to the chamber itself. Then the system should be pressure
tested. And since the manufacturer of this particular CPD unit (e.g.
Polaron) has had on the order of thirty years of experience doing this kind
of testing, on this particular design of vessel, I would recommend having
them do the testing for you in the UK. Today the testing of the Polaron
unit is done to the CE standard, without doubt the most stringent in the
world.

If you do not have the original test certificate, if you send me your S/N I
could try to get a copy of it for you. That alone might satisfy the
questions being asked of your safety people.

Disclaimer: SPI Supplies has offered this particular CPD unit, especially
to customers outside the USA, for nearly twenty five years and we are
unaware of any requirement to have the vessel retested at periodic intervals
for safety reasons.

Chuck


============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.spi.cc
########################
============================================




From daemon Wed Mar 29 17:30:32 2000



From: Derrick Calandra :      calandra-at-atfinet.com
Date: Wed, 29 Mar 2000 09:59:59 -0600
Subject: seeking equip.

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I'm looking for some used equipment to set up a small lab space. Any
recommendations would be great.

1. inverted microscope, for TEM sample prep
2. hot plate
3. diamond saw

Thanks in advance.

Derrick



From daemon Wed Mar 29 17:30:33 2000



From: Ginger R Hendricks :      lizard-at-osu-com.okstate.edu
Date: Wed, 29 Mar 2000 10:15:43 -0600
Subject: Immunogold enhancement method questions

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Hello all,

I am passing this on for a fellow colleague. Thank you in advance.

Ginger (Baker) Hendricks
EM Lab Manager
Oklahoma State University College of Osteopathic Medicine


"I am looking for an immunogold-silver enhancement method for labelling a
cytoplasmic antigen in cultured cells and viewing with Light Microscopy.
We will be using Protein Aand a monoclonal antibody. Once we have
determined that we have specific labelling, we will proceed to visualizing
the gold with TEM."



From daemon Wed Mar 29 17:30:36 2000



From: Dr. Tina Schwach :      tschwach-at-barrishind.com
Date: Wed, 29 Mar 2000 11:45:23 -0600
Subject: Re: Preparation of tissue culture cells for TEM

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} Hello everyone,
} I have a student who wants to prepare tissue culture cells for TEM in Agar
} blocks. I shall appreciate any suggestions.
} Thanks in advance.
} C.L.Singla
}
It would be helpful to know how the tissue culture cells are being
grown...in plastic wells where they will be scraped off or on plastic or
glass coverslips where the entire surface will be embedded. What is the
ultimate goal of the TEM examination...morphology, interactions? Will the
samples be ultimately embedded in plastic or expoxy?

Dr. Tina Schwach
Microscopy Consulting Services, Inc.



From daemon Wed Mar 29 17:30:36 2000



From: Corazon Bucana :      bucana-at-audumla.mdacc.tmc.edu
Date: Wed, 29 Mar 2000 12:01:41 -0600
Subject: SEM and TEM of spores

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I would appreciate it very much if someone can refer me to a good book or
protocol for preparation of fungal spores for SEM and TEM. This is very
foreign to me since I have been working with mammalian tissues exclusively
for so many years but now that the lab is a core facility I am getting somel
requests for other types of samples. Thank you,

Cora Bucana
*******************************************************
Corazon D. Bucana, Ph.D.
Department of Cancer Biology
U.T. M.D. Anderson Cancer Center
1515 Holcombe Blvd. Box 173
Houston, Texas 77030
Phone: (713) 792-8106
FAX: (713) 792-8747
Email:bucana-at-audumla.mdacc.tmc.edu
FAX: (713) 792-8747



From daemon Wed Mar 29 17:30:37 2000



From: John Heckman :      heckman-at-pilot.msu.edu
Date: Wed, 29 Mar 2000 13:37:03 -0800
Subject: Re: critical point drying apparatus

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christine richardson wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hi,
} Our safety people are concerned about having our Polaron c.p.d
} pressure tested at regular intervals.
} Does any one out there know of anyone who does this sort of thing,
} preferably on site?
} Also I would be interested to hear how other users safety check this
} sort of equipment.
} thanks,
} Christine.

Hi Christine,

Over here in the US we have an Interstate Commerce Commission that checks
pressure vessels via hydrostatic testing. This is required every 5 years
and the tops of gas cylinders, at least, are stamped with the last test
date. It's always amazed me just how long N2 cylinders last (reading
hydrostatic test dates is something to do while your plates are
processing). I've seen bottles with dates that were before W.W.I. All that
age and 2250 psi. I've never heard of them requiring the testing of lab
equipment, though. Might check with your bottled gas supplier to see if
they'd do it.

cheers,
John



From daemon Wed Mar 29 17:30:37 2000



From: HILDEGARD CROWLEY :      hcrowley-at-du.edu
Date: Wed, 29 Mar 2000 11:46:11 -0700 (MST)
Subject: Re: Epon sections

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On Wed, 29 Mar 2000, Anne Heller wrote:

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}
} We work with plant material and after ultra thin sectioning our sections
} show lines of compressions in cell wall areas. Of course, we try to
} stretch the Epon sections with a heat pen or with chloroform, but very
} often we are not successful. The sections just move on the water
} surface and nothing is happening. Any advice would be gratefully
} appreciated.
} Regards,
} Anne Heller
} --
} Dr. Anne Heller, AG Elektronenmikroskopie,
} Institut für Botanik (210), Universität Hohenheim,
} Garbenstraße 30
} D-70593 Stuttgart
}
} Tel.: (0049)-711-459-2180
} Fax.: (0049)-711-459-3355
}
}
}
}
Hi,

If I get any reaction in a section floating in a boat from xylene, heat
pen, chloroform, etc., I know that something is wrong. My personal test
of a good embedment is nonreactivity of floating sections. Why?

In industry, if it is important that there are no free monomers in the
section, the material is exposed to the above solvents to soak them out.

If you have enough unpolymerized monomers in your section, solvents will
affect that section. (Note: Embedments that are too soft in formulations
are excluded from this discussion at this time).

So - keep your formulations well mixed at all times - do not allow it to
sit in the hood. It must be in motion at all times. Push infiltration!
More changes, longer times. If using epoxy of any sort, heat the
infiltration medium to 37 deg C for 1 hour after every new change of
resin while the sections are on the rotator. A plain 60W light bulb is
ideal for this. Polymerize well. At least for 48 hours. If needed,
repolymerize at 95 deg C for an hour especially if your formulation
contains NMA.

Your formulation may be wrong for your material. Try going harder. Also
with existing blocks, try reheating, then try a lower knife angle and a
slower speed of cutting.

Most important: Keep records. Know what you are doing and why. Do not
haphazardly try this, try that. You will get crazy! Embedments are very
complex systems that belong into the realm of material science - that is
why biologists have so much trouble with them. (I have had mega fights
lasting years with epoxies)

Good luck,

Hildy Crowley
University of Denver
Denver, CO



From daemon Wed Mar 29 17:30:44 2000



From: Alan G. Fox :      Alan.Fox-at-uts.edu.au
Date: Thu, 30 Mar 2000 08:23:19 +1000
Subject: Re: Analytical TEMS - The choices

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Keith
If you have a new FEG machine with a Schottky emitter, then these are
relatively easy to operate and fairly robust, but they do, of course, cost a
lot more and so you may not want to spend that money and have ham-fisted users
operating it. For high-resolution analytical TEM/STEM there is no doubt that
FEG is the preferred source. The small source size and small energy spread
combined with high gun brightness gives great performance. A word of
warning,though. When using a GIF with any TEM it is often necessary to operate
at low mag because of the additional mag (18 - 20 times) introduced by the
GIF. In this situation (depending on the mag), the LaB6 source may be
preferable in certain situations because the total current is higher than the
FEG source. I have used both types of machine-Schottky FEG plus GIF and LaB6
plus GIF and there do not appear to be any severe problems with the low mag
mode operating with a Schottky source. The cold FEG source may not be so good
from this point of view, since the source size is smaller than the Schottky
FEG and so the cross-over probe size above which the current in the cold FEG
probe is less than the LaB6 is small and probably in the range 10-100 nm. I
have not used a cold FEG TEM with a GIF such as the Hitachi and so I can't
really make an informed comment, but Profs Dravid and Marks at Northwestern
University are very skilled users of the Hitachi machine and they can surely
advise you. Best of luck.

Alan Fox
Center for Materials Science and Engineering
Naval Postgraduate School
Monterey
California 93940


Keith Moulding wrote:

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} Greetings,
}
} We are contemplating replacing our analytical TEM. A basic spec we envisage
} is STEM, EDS + GIF/PEELS. We are happy to choose the EDS, but we run into
} trouble with e-guns, i.e. to go LaB6 or FEG. In particular as we would like
} to bolt a GIF/PEELS on to the system.
}
} We have several concerns:
} The safety of the FEG gun, considering we are a multi-user facility with
} users who have a frequent tendency to bend holders and press the wrong
} buttons. How problematic are FEGs, do you have much down time / running
} cost due to careless users?
}
} GIF/PEELS: has anybody carried out a systematic comparison between a system
} with LaB6 or FEG. Is the extra cost of a FEG (over twice the price) really
} justified for GIF/PEELS or will it quite happily run on a LaB6.
}
} The thoughts of fellow microscopy in the same quandary much a appreciated.
}
} Keith.
}
} ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
} Dr. K. Moulding.
}
} Materials Characterisation and Preparation Facility
} Hong Kong University of Science and Technology,
} Clear Water Bay,
} Kowloon,
} Hong Kong.
}
} FAX: (852) 2358 2451
} TEL: (852) 2358 8724
} ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~



From daemon Thu Mar 30 07:28:13 2000



From: jim :      jim-at-proscitech.com.au
Date: Thu, 30 Mar 2000 10:04:24 +1000
Subject: FW: critical point drying apparatus

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Phone a divers shop and ask who is testing their cylinders. Those people can
also test the CPD. It would be a hydrostatic test and they would need to have a
fitting to connect to the back of the CPD. To have any meaning the applied
pressure would need to be higher than normally applied, I suggest by 50%.

I think its a bad idea to do such a test. The metal housing of those units is
vastly over-designed and the window is quartz which ultimately would crack but
not shatter. All the test would achieve is to burn up some of your funds and
time. Stress the system and if your unlucky crack the window - would the
"safety people" pay for that?

It would be interesting to learn how many systems have failed and if there were
any special circumstances. I don't know of any instance of a CPD failure.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com


Hi,
Our safety people are concerned about having our Polaron c.p.d
pressure tested at regular intervals.
Does any one out there know of anyone who does this sort of thing,
preferably on site?
Also I would be interested to hear how other users safety check this
sort of equipment.
thanks,
Christine.




From daemon Thu Mar 30 07:28:17 2000



From: Pnina Ari-Gur :      arigurp-at-wmich.edu
Date: Wed, 29 Mar 2000 21:26:12 -0500
Subject: Position Announcement

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ASSOCIATE DEAN, COLLEGE OF ENGINEERING AND APPLIED SCIENCES

Western Michigan University seeks applications and nominations for the
position of Associate Dean for Undergraduate Programs and Assessment of
the College of Engineering and Applied Sciences.

Western Michigan University is a Carnegie Doctoral I university with 750
FTE tenure-track faculty and an enrollment of about 27,000 students, 25%
at the graduate level. It is advancing to Carnegie Research II
classification and plans a substantial investment in the College of
Engineering and Applied Sciences, including a new physical facility, to
accomplish this goal. In addition to its Graduate College and Lee
Honors College, Western supports seven degree-granting colleges: Arts
and Sciences, Haworth College of Business, Education, Engineering and
Applied Sciences, Aviation, Health and Human Services, and Fine Arts.
These colleges offer 242 academic programs, including 60 at the masterâs
level and 25 at the doctoral level.

The Associate Dean for Undergraduate Programs and Assessment reports to
the Dean. This person provides leadership and oversees the development
of college policy for undergraduate programs, accreditation, assessment,
advising, recruitment, retention, student orientation, co-op, and intern
programs. An earned terminal degree in one of the disciplines in the
college or a related science is desirable. A doctoral degree, with at
least one degree in an engineering or closely related
science/engineering discipline, is required. University experience in
academic programs and demonstrated excellence in teaching are required.
Candidates should have excellent administrative and interpersonal skills
and experience utilizing instructional and administrative technology.
An excellent record of teaching and research and/or creative achievement
that would warrant an appointment as associate or full professor with
tenure in one of the academic units in the university is required.

The individual selected will assume academic and administrative
responsibilities for undergraduate programs in a dynamic and growing
college offering 16 baccalaureate, 11 masterâs and three doctoral
programs. The collegeâs staff includes 70 full-time faculty, 10
administrators (8 with faculty rank), 31 funded staff positions, and
dozens of contract staff and graduate student assistants, and teaches
approximately 2300 students. The college has a strong commitment to
education and research, which spans a broad spectrum of engineering and
engineering technologies. The college offers courses and programs
primarily on Western Michigan Universityâs main campus in Kalamazoo, but
also serves as a major resource for off-campus instruction and economic
growth at fives sites across West Michigan.

Candidates should submit 1) a curriculum vita, 2) three letters of
recommendation, 3) an application letter stating their qualifications
for the position, and 4) a statement outlining a personal vision for
engineering education to:

Parviz Merati, Chair
Associate Dean Search Committee
Department of Mechanical and Aeronautical Engineering
Western Michigan University
Kalamazoo, MI 49008

Review of applications will begin on or about May 1, 2000. Applications
will be accepted until the position is filled. Western Michigan
University is an equal opportunity /affirmative action employer.

For additional information about WMU and the College of Engineering and
Applied Sciences, refer to our Website: http://www.wmich.edu/.
=====================================
Pnina Ari-Gur, D.Sc., Professor
Materials Science and Engineering
CMD Department
Western Michigan University
Kalamazoo, MI 49008
(616) 387-3372 FAX: (616) 387-6517
email: pnina.ari-gur-at-wmich.edu
http://www.wmich.edu/cmd/arigur.htm
=====================================




From daemon Thu Mar 30 07:28:29 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Wed, 29 Mar 2000 20:42:05 -0600
Subject: Leaf Microlumina

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This is on ebay now. Here is the URL:

http://cgi.ebay.com/aw-cgi/eBayISAPI.dll?ViewItem&item=296173307

Not announced on ebay, and limited to MSA, I am offering
an IBM Thinkpad 355 which is optionally available to make this camera truly
portable. $350 extra gets the Thinkpad. You will still need a Slim SCSI
PCMCIA card, but these are very low cost. I have one in a Sony VAIO
and it works great with the Leaf.

Any questions, please ask.

gary g.



From daemon Thu Mar 30 07:28:35 2000



From: Divakar R :      divakar-at-igcar.ernet.in
Date: Thu, 30 Mar 2000 12:01:13 +0530
Subject: EM image simulation software

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I need information related to EMS software. There was a related posting (enclosed below) recently, replies to which I seem to have missed. My mail to the author of that posting also bounced back. Could somebody please help me get this info? Thanks very much.
----
Divakar R
Physical Metallurgy Section, Indira Gandhi Centre for Atomic Research
Kalpakkam 603102, India
----



-----Original Message-----
} From: Divakar R [SMTP:divakar-at-igcar.ernet.in]
Sent: Wednesday, March 29, 2000 8:23 PM
To: 'Chengge Jiao'


Hi,

Does anybody know the price for following softwares for EM simulation.

1. Diffraction 2.0 (or the most latest version),

2. EMS.

Chengge Jiao

H.H.Wills Physics Laboratory
University of Bristol, UK

c.g.jiao-at-bristol.ac.uk








From daemon Thu Mar 30 07:28:37 2000



From: Ron Doole :      ron.doole-at-materials.oxford.ac.uk
Date: Thu, 30 Mar 2000 08:32:25 +0100 (BST)
Subject: Re: TEM:Analytical TEMs

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Hi Keith,

In the matter of inexperienced users and FEG versus Lab6 I have no fears
on our FEGTEM.

We have been running our JEOL3000F for over a year now without any
trouble, it has a Schottky emitter so there is no flashing to be carried
out. The emitter is the original one used for factory tests, shipped
over, used for installation and still in use. We have experience of
several LaB6 instruments over the past 15-20 years and are a materials
science multi-user facility.

The FE gun runs 24 hours a day, the user just opens the valve to see the
beam. The gun and emitter are run up by an experienced person whenever it
needs it (every 2-3 months). We have a `Quick Emission Selector' that we
have set up to give 3 more emission currents (higher for GIF, lower for
less energy spread etc.) and the user cannot readjust these only select
between them. There is no room for abuse (even for some of our users!).

The LaB6 guns have to be run up at the start of every session and every
time a user changes specimen or films. After a period of time the LaB6
tips slowly deposit insulating LaB6 on the Wenhelt, this charges
and the bias has to be adjusted to reset the emission correctly. When a
user finishes the session this charge dissipates and the next user gets a
high emission current which has to be reset using the bias. The bias has
to be adjusted during the first part of the session as the charge builds
up. This gives the user lots of room for abusing the tip, it is not
possible to prevent access to the bias control as they do need to use it.

Regards,
Ron

===========================================================================
Mr. Ron Doole e-mail ron.doole-at-materials.ox.ac.uk
Department of Materials, phone +44 (0) 1865 273701
University of Oxford, fax +44 (0) 1865 283333
Parks Road.
Oxford. OX1 3PH. UK.
============================================================================



From daemon Thu Mar 30 07:28:37 2000



From: Gunnar Kopstad :      gunnar.kopstad-at-medisin.ntnu.no
Date: Thu, 30 Mar 2000 09:28:45 +0200
Subject: Critical point drier, window cracking

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hi,

Two days ago, the window in our CPD cracked. The run was in every respect
normal, but the window cracked at 35 degrees/80 bar. This was a big
surprise to us, as we have belived that this could never happen as long as
temperature and pressure are within their normal ranges. We would be very
interested to receive comments on this.

The week before, the equipment, which is more than 20y old, had been
disassembled for cleaning and replacement of the window O-ring.

Best Regards, Gunnar Kopstad.


Vennlig Hilsen
dr.ing Gunnar Kopstad
overingeni¿r Avd f Patologi, Rit

tlf. 73 86 86 56, tlf. privat 72 88 79 58
pers.s. 967 75 026



From daemon Thu Mar 30 07:28:38 2000



From: Keith Ryan :      KPR-at-wpo.nerc.ac.uk
Date: Thu, 30 Mar 2000 08:33:52 +0100
Subject: Re: FW: critical point drying apparatus - life of?

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For the record - and maybe this is a record - our Polaron E3000 CPDA
was tested in Feb. 1974 to a proof load of 2,500 lbf/in2. Under
"Remarks" on the test certificate it says "Proof pressure applied
without any visual signs of distortion or leakage. Safety valve set at
1,900 lbf/in2."

I take "lbf/in2" to be "pounds per square inch".

The unit has been in quite regular use since 1974 with no problems
other than failure of seals. I did once have to de-"fur" the
waterways but that was a local water supply problem.

Keith Ryan
Marine Biological Association
Plymouth UK



From daemon Thu Mar 30 07:28:39 2000



From: Petra Wahlbring :      wahlbrin-at-crpgl.lu
Date: Thu, 30 Mar 2000 10:19:20 +0200
Subject: Re: EFTEM/GIF on particles

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At 16:26 29.03.00 +0200, you wrote:

..

} I tried to do EELS measurements a few times and got very few succes. The best
} results are obtained on beam resistant samples simply because I can then move
} the zero loss peak off the CCD and increase intensity (or acq time) at
will. I
} start with a very low intensity and optimise it in turboview mode to get a
} good signal out. However I could seldom see well defined peaks.
} One point which I find strange is that we can often get reasonnably good
} elemental maps even when we don't see any peak in the EELS spectrum. The
} question is then if the map is really trustworthy...
}
} Any comments welcome !
}
} Olivier

With my experience I think it is rather normal than surprising that you can
achive relatively good elemental maps in ESI mode but see no recognizable
features in the EELS spectrum.
With an elemental map you get energy loss information for each single image
point. If there is a higher concentration of a certain element, the
intensity of the corresponding pixel in the energy window on the edge will
be higher than in neighbouring pixels not containing this element or only
in less quantity. This does not necessarily mean that you would actually
see a peak in the corresponding spectrum of this pixel, it can be just a
change in the slope of the background. Since you are comparing the changes
in intensity of every pixel this small differences become visible in an
map. Furthermore, in spot mode or selecting a small area with an aperture,
you will average usually over a larger area than a pixel in image mode. If
the features of your specimen are smaller the jump ratio of the edge will
be reduced due to the contribution of the surrounding material.
If you thoroughly compare spectra acquired on a (large enough) area
containing the element of interest and a spectrum of the matrix you will
find differences.
Furthermore you will see certainly more of the edges in your spectra of
thick specimens if you go for a deconvolution. It is still surprising (at
least for me) how much information you can gain from a spectrum which did
not look much more than a steady decrease in intensity.

Petra

--------------------------------------------------------------
Dr. Petra Wahlbring
Centre de Recherche Public - Gabriel Lippmann
Laboratoire d'Analyse des Materiaux (LAM)
162a, av. de la Faiencerie L-1511 Luxembourg
tel. +352-466644-402 fax +352-466644-400
e-mail: petra.wahlbring-at-crpgl.lu
Visit our WWW site! http://www.crpgl.lu/~wahlbrin



From daemon Thu Mar 30 07:28:45 2000



From: Anne Heller :      heller-at-Uni-Hohenheim.DE
Date: Thu, 30 Mar 2000 10:57:55 +0200
Subject: Re: SEM and TEM of spores

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Corazon Bucana schrieb:

} ------------------------------------------------------------------------
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} -----------------------------------------------------------------------.
}
} I would appreciate it very much if someone can refer me to a good book or
} protocol for preparation of fungal spores for SEM and TEM. This is very
} foreign to me since I have been working with mammalian tissues exclusively
} for so many years but now that the lab is a core facility I am getting somel
} requests for other types of samples. Thank you,
}
} Cora Bucana
} *******************************************************
} Corazon D. Bucana, Ph.D.
} Department of Cancer Biology
} U.T. M.D. Anderson Cancer Center
} 1515 Holcombe Blvd. Box 173
} Houston, Texas 77030
} Phone: (713) 792-8106
} FAX: (713) 792-8747
} Email:bucana-at-audumla.mdacc.tmc.edu
} FAX: (713) 792-8747

Dear Cora Bucana,

it is depending very much on the type of spore, e.g. mildew with high water
content and "thin" walls is easy to prepare with standard protokolls for plant
material. Dormant, thick walled spores with less water and high lipid content
are quite tricky to prepare for TEM, but easy for SEM. I can send you a detailed
protocol if you tell more about your fungal spores.

--
Dr. Anne Heller, AG Elektronenmikroskopie,
Institut fŸr Botanik (210), UniversitŠt Hohenheim,
Garbenstra§e 30
D-70593 Stuttgart

Tel.: (0049)-711-459-2180
Fax.: (0049)-711-459-3355




From daemon Thu Mar 30 07:28:47 2000



From: Robin Cross :      r.cross-at-ru.ac.za
Date: Thu, 30 Mar 2000 11:39:50 +0200
Subject: Re: CPD safety question

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On 28 Mar 00, at 10:09, Garber, Charles A. wrote:

} ----------------------------------------------------------------------
} -- The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------
} -.
}
}
} -- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --
}
} Christine Richardson wrote:
} ===================================================
} Hi,
} Our safety people are concerned about having our Polaron c.p.d
} pressure tested at regular intervals.
} Does any one out there know of anyone who does this sort of thing,
} preferably on site? Also I would be interested to hear how other users
} safety check this sort of equipment.
} =================================================== I might not be the
} last word on this, but I had described to me the kind of large liquid
} filled tank (I think it is water) that pressure vessels like this are
} tested in, and to me at least it did not sound like it would be the
} kind of thing that one would "carry around" in a portable kind of unit
} to do this kind of testing, on site. This description came, some
} years ago, from Dr. Wilf Gee (now deceased) and who was very much
} involved in the original manufacturing of this product and who had
} involvement for many years with the safety testing of the unit.
}
} When your CPD unit was first delivered, it would have come with a copy
} of a report from the testing agency showing the results and conditions
} of the pressure testing. I might be off by a bit on this, but it was
} my recollection that the vessel is tested to a pressure level that is
} three or four times higher than what would be required to blow out the
} rupture disc. In other words the unit is tested to a level that, if
} the rupture disc did not blow exactly where it was supposed to blow,
} there is some very large margin of error so that the disc still would
} blow long before the vessel.
}
} There are probably more CPD units of this design installed in the
} world than any other design. I have never heard of anyone having any
} kind of a problem with it (e.g. an explosion), except an occasional
} blowing of a rupture disc, but certainly not the pressure vessel
} itself. Just don't ever try to run the unit by replacing the rupture
} disc, if one is not handy, with a cut metal disc. That is very
} dangerous and should never be done. Believe it or not, we do uncover
} once in a while, people who do do that sort of thing, out of naivety
} of course and that is why I do not waste an opportunity to point out
} the danger in doing that sort of thing. That is why I always have
} recommended, for multi-user environments in particular, to always have
} a small supply of replacement rupture discs near by the unit just to
} avoid even the slightest temptation (such as by a student) to by-pass
} this important safety feature.
}
} So I for one would be very interested in hearing the logic and
} rationale of your safety committee that is of the opinion that you
} should have your pressure vessel tested from time to time.
}
} However, there is one kind of situation where one should have the
} vessel pressure tested and that is if one has attempted any mechanical
} modifications to the chamber itself. Then the system should be
} pressure tested. And since the manufacturer of this particular CPD
} unit (e.g. Polaron) has had on the order of thirty years of
} experience doing this kind of testing, on this particular design of
} vessel, I would recommend having them do the testing for you in the
} UK. Today the testing of the Polaron unit is done to the CE
} standard, without doubt the most stringent in the world.
}
} If you do not have the original test certificate, if you send me your
} S/N I could try to get a copy of it for you. That alone might satisfy
} the questions being asked of your safety people.
}
} Disclaimer: SPI Supplies has offered this particular CPD unit,
} especially to customers outside the USA, for nearly twenty five years
} and we are unaware of any requirement to have the vessel retested at
} periodic intervals for safety reasons.
}
} Chuck
}
}
} ============================================
}
} Charles A. Garber, Ph. D. Ph: 1-610-436-5400
} President 1-800-2424-SPI
} SPI SUPPLIES FAX: 1-610-436-5755
} PO BOX 656 e-mail:cgarber-at-2spi.com
} West Chester, PA 19381-0656 USA
} Cust.Service: spi2spi-at-2spi.com
}
} Look for us!
} ########################
} WWW: http://www.spi.cc
} ########################
} ============================================
}
}
}



Robin H Cross
Director : EM Unit, Rhodes University, Grahamstown, South Africa
R.Cross-at-ru.ac.za
tel: +27 46 603 8168 - fax: +27 46 622 4377
http://www.ru.ac.za/emu/index.htm

** remember that ICEM-15 in 2002 is in Durban, South Africa **



From daemon Thu Mar 30 07:28:48 2000



From: Robin Cross :      r.cross-at-ru.ac.za
Date: Thu, 30 Mar 2000 11:53:57 +0200
Subject: Re: CPD safety question

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Hello Chuck, and others interested

Firstly, apologies for the earlier reply that was sent before I gave
my comments!

} There are probably more CPD units of this design installed in the
} world than any other design. I have never heard of anyone having any
} kind of a problem with it

We have used two of these units for more than 25 years and the
only problems we have experienced, other than seals which have
had to be replaced from time to time, have been one ruptured disc
and a leak which developed in the supply pipe from the CO2
cylinder. The latter was repaired (new one made up using existing
connectors fitted to new high pressure piping) by our local
compressed gas supplier.

Many years ago, having been told that pressure chambers should
be checked from time to time, I sent one of our units back to be
tested by Polaron in the UK. It came back with a certificate issued
by Probe Technical Services saying that it had been tested to 2500
psi with the comment " Proof pressure applied for one minute
without any visual sign of leakage or failure".

Regards

Rob



Robin H Cross
Director : EM Unit, Rhodes University, Grahamstown, South Africa
R.Cross-at-ru.ac.za
tel: +27 46 603 8168 - fax: +27 46 622 4377
http://www.ru.ac.za/emu/index.htm

** remember that ICEM-15 in 2002 is in Durban, South Africa **



From daemon Thu Mar 30 07:28:49 2000



From: chris smith :      chris.smith-at-bbsrc.ac.uk
Date: Thu, 30 Mar 2000 12:09:37 +0100
Subject: CPD safety

Contents Retrieved from Microscopy Listserver Archives
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Hi Christine,
We have a Polaron E300 but no demand for routine testing from our 'safety
people' presumably because they know that the burst disc will go long before
the body starts to creak. After a major re-fit it was sent to testing
specialists who gave it a hydrostatic test to 2500 psi for one minute for a
safe working pressure of 2000 psi. It must be possible to do this on site, I
cant imagine big autoclaves or air compressor reservoirs being posted off
for their tests.
It is not as exciting as it sounds. Holes are blanked off and then you pump
it full of water so faults are revealed as leaks rather than the more
entertaining earsplitting bang and shower of shrapnel which could result
from the use of a compressible medium.
If the safety people twist your arm try the Yellow Pages under Engineers,
Inspection & Testing.
ttfn, chris.smith-at-bbsrc.ac.uk
Plant Path Dept., IACR-Rothamsted, Harpenden, Herts. UK.



From daemon Thu Mar 30 07:28:51 2000



From: Brian Robertson :      brian.robertson-at-materials.oxford.ac.uk
Date: Thu, 30 Mar 2000 13:29:07 +0100
Subject: Re: Critical point drier, window cracking

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Dear Gunnar
It is most likely that there was at least one surface scratch
or microcrack that was loaded in a way that resulted in major crack
growth, whose result you observed. During glass removal and
replacement, there were probably several actions that could have
contributed to this -- scratching during cleaning or tool use, altered
clamping of the glass, or turning a scratch on one side of the glass
from inside to outside (or the reverse). Did anyone clamp the glass in
a different way on replacing it, compared with before? (so that the
applied stresses are less uniform or at least different, from before?)
Moisture is well-known (scientifically and by all who specialize in
cutting glass) to enhance crack growth when some component stress
applied to the crack tip region helps to open the crack. All these
could contribute to the failure. The timing of the failure indicates
that one or more of these factors most likely resulted in cracking.
Best wishes for avoiding the problem for another 20 years!
Brian Robertson

------------------
Brian Robertson
Assoc. Prof., Mechanical Engineering, University of Nebraska-Lincoln,
on sabbatical at
Department of Materials, University of Oxford,
Parks Road, Oxford OX1 3PH, United Kingdom
FAX 44+ 1865 273794
brian.robertson-at-materials.ox.ac.uk

* This e-mail message was sent with Execmail V5.0.x *



From daemon Thu Mar 30 17:54:42 2000



From: Jonathan Barnard :      Jonathan.Barnard-at-Angstrom.UU.SE
Date: Thu, 30 Mar 2000 14:44:59 +0100
Subject: TEM:EFTEM of particles

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Olivier,
I find it a bit bizarre that you cannot see our mapped features in your
EELS. You're not mapping multiply scattered plasmons are you (a problem
with lower lying edges)? How are you acquiring the EEL spectrum:
Diffraction pattern (spot/CBED)? Or using the image? What sort of count
rates are you getting in the pre-edge images and the maps? Are you sure it
is not noise?

For EFTEM practice, I would if possible, try getting hold of some Al or Mg
particles/powder (not smoke cubes), preferably mixed, and play with imaging
the various plasmons i.e. see which energy windows are best and if possible
see if you can image the oxide layers. It's fun because the signal is
pretty high so easy to see.

Going to core-loss EFTEM the main problems are resolution: For low loss
edges its spatial resolution (mainly delocalization) although there is
evidence that this is not as restrictive as it looks on paper (see Z.L.Wang
Ultramic Vol 67 (1997) pp105-111 for a demonstration in Al).

For higher edges the signal resolution (object resolution function as
Berger & Kohl call it) tells you how good the statistics in a map are. A
good paper is A.Berger & H.Kohl (Optik Vol:92 (1993), No4 pp175-193) which
tells you about both spatial and signal resolution. The equations are quite
rigorous, but it will show you how to get started measuring the
signal-to-noise ratio (SNR). With a bit of practice you can then produce
some SNR plots like those in Hofer, Grogger, Kothleitner & Warbichler
(Ultramicroscopy 67 (1997)pp83-103) which tell you where the energy slit
should be positioned to optimise SNR for a given slit width.

I suppose the best advice is find an easy material to practice with and
work from thereon.
Good luck and have fun.

Jonathan
********************************************************
Dr Jonathan Barnard

Analytical Materials Physics
The Angstrom Laboratory, Uppsala University
P O Box 534, SE-751 21 Uppsala, Sweden
Phone: +46-(0)18-4716838 Fax: +46-(0)18-500131
http://www.angstrom.uu.se/analytical/home.html

********************************************************



From daemon Thu Mar 30 17:54:44 2000



From: anderron-at-us.ibm.com
Date: Thu, 30 Mar 2000 09:17:56 -0500
Subject: Operator Horror Stories

Contents Retrieved from Microscopy Listserver Archives
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Alan Fox, in a response to the Analytical TEM string, cautioned the
question-raiser to beware of people who abuse instruments. Perhaps Nestor
will forgive a little microscopy related humor and allow us to start a
string on "Operator Horror Stories." Here's ours:

The "ham-fisted user" reference made us chuckle/cringe with the memory of
a guest "microscopist" in our lab who hauled himself (220 pounds or so) out
of his seat by pulling on the half-inserted specimen rod, bending it about
20 degrees or so!

Henceforth, when we saw him in the hall (he never came into the scope room
again), we referred to him as "Conan the Microscopist"!



Ron Anderson, IBM, Hopewell Jct., New York, USA. anderron-at-us.ibm.com

IBM Analytical Services; http://www.chips.ibm.com/services/asg





From daemon Thu Mar 30 17:54:50 2000



From: Henry Eichelberger :      heichelb-at-binghamton.edu
Date: Thu, 30 Mar 2000 12:11:38 -0500
Subject: FW: TEM, Biological, sinking of tissue in sucrose

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Marie:
You didn't mention the fixative and length of time fixed. Could it be
possible that the tissue is "too well fixed" to allow easy exchange
solutions? Also, have you tried thinner vibratome sections? We routinely
use 40 micron thick brain sections for immuno labeling.
Best regards,
Henry
***********************************************
Marie E. Cantino wrote:
We are preparing perfusion fixed brain tissue by freeze substitution for
post-embedding immuno gold. Following fixation we prepare 500 micron
vibratome sections, which we then wish to infiltrate with 2 M sucrose in
buffer prior to freezing. We have been transferring the brain sections to
1 M sucrose, then when they sink, transferring again to 2 M. The problem
is that the sections never sink in the 2 M sucrose.
*****************************
Henry Eichelberger, Manager
Electron Microscopy Facility
Department of Biological Sciences
Binghamton University
Binghamton, NY 13902-6000

phone: (607) 777-2682
fax: (607) 777-6521
e-mail: heichelb-at-binghamton.edu




From daemon Thu Mar 30 17:54:50 2000



From: Andrew Coutermarsh :      acoutermarsh-at-omegafilters.com
Date: Thu, 30 Mar 2000 12:09:42 -0500
Subject: JOB POSTING

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FOR THOSE WHO MISSED THIS THE FIRST TIME:

JOB POSTING

Company: Omega Optical, Inc.

Location: Brattleboro, Vermont

Company
Description: 30-year old designer and manufacturer of thin film optical
interference filters used world-wide for laboratory and scientific
equipment.

Position: Fluorescence Product Manager

Position
Description: The Fluorescence Product Manager is responsible for any and
all activities which help further the companyâs sales of fluorescence
products. These activities include:

1. Technical sales and sales support: Technical sales and assistance to
sales force regarding fluorescence applications.
2. Marketing: Marketing activities related to fluorescence products.
3. Product & Market Development: Development activities related to new
fluorescence products, applications, and developing markets.

Omega is seeking an individual who is excited about working with varied
applications of fluorescence detection in the life sciences. The work
involves discussing the best match between laboratory experiments and
Omegaâs wide range of light filters for this science. Experience in
fluorescence, microscopy, life sciences and instrumentation is
essential.

Salary: Competitive with full benefit package.

Contact
Information: Email response with attached Word file resume and salary
history to: acoutermarsh-at-omegafilters.com or snailmail response to:


Andrew Coutermarsh, SPHR, HR Director
Omega Optical, Inc.
PO Box 573
Brattleboro, VT 05302


--
================================================================================

"If I am not for myself, who will be? If I am only for myself, what am
I? If not now, when?"
----Hillel




From daemon Thu Mar 30 17:54:52 2000



From: Paula Allan-Wojtas :      ALLANWOJTASP-at-EM.AGR.CA
Date: Thu, 30 Mar 2000 13:02:03 -0500
Subject: LM/EM Embedding Oven

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Hi, All,

I am posting a message for a colleague who does not subscribe to this list.

He is looking for small embedding oven which can polymerize resins in the range of 40¡-70¡C, but which has a very precise temperature control (holds the temperature well, with very little fluctuation during polymerization). He is having problems with the oven he presently has because the temperature is not controlled well enough, and he is getting uneven polymerization.

Another condition is that he is in the middle of a project and needs the oven right away. He had an oven in mind, but it could not be delivered for 6 weeks!!!

Any suggestions from users or vendors are welcome. Please contact me offline, and I'll forward the replies to my colleague.

Thanks again for all your help.

Paula.

Paula Allan-Wojtas
Research Scientist, Food Microstructure
Agriculture and Agri-Food Canada
Atlantic Food and Horticulture Research Centre
Kentville, Nova Scotia Canada B4N 1J5

Tel: (902) 679-5566
FAX: (902) 679-2311

email: allanwojtasp-at-em.agr.ca



From daemon Thu Mar 30 17:54:54 2000



From: C. Singla :      csingla-at-uvic.ca
Date: Thu, 30 Mar 2000 10:18:35 -0800
Subject: Rupture disc for Bomar CPD

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Hallo friends,
I have a Bomar SPC 1500 critical point dryer and I am looking for the
rupture disc size or part number and a vendor. I looked in the Bomar manual
but I can't find any information about it. I shall very much appreciate any
information about it.
Thanking you in advance.
C.Singla



From daemon Thu Mar 30 17:54:55 2000



From: HILDEGARD CROWLEY :      hcrowley-at-du.edu
Date: Thu, 30 Mar 2000 11:20:35 -0700 (MST)
Subject: Stretch?? Epoxy sections?

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Hi,

I agree with the disagreement that has been raised to my posting of my
personal criteria for good embedment. (A section that stretches in the
boat on exposure to solvent fumes is suboptimal and the embedment needs
changing.)

I have not dealt much with plant material - mycobacterium is the closest I
have come to dealing with cell walls which are tough as Michelin tires. I
also have not used glass knives for more than a decade. Even my students
start out on an old diamond knife. I am no longer familiar with the
vagrancies of glass knives (every one of them seems to have a different
cutting edge! Never twice the same!).

Certainly epoxies belong to the organic chemists. But they also belong to
the material scientists. I have in front of me a thesis done under the
mentoring of Dr. Giammara - A Material Science Evaluation of Epoxy Systems
for TEM Applications. At one time I got into a huge fight with embedding
filters of certain types. I had a computer program which calculated for
me using WPE numbers and molecular weights 36 different formulations. I
actually investigated 25 of them. Totally desperate (and mad) I started
attending the material science symposia on polymers at the MSA meetings.
I found this helpful.

My second favorite book is, HANDBOOK OF EPOXY RESINS. I adore it. (This
has caused my friends to question my sanity and make peculiar remarks)
This book has been of enormous help in my various battles (lots of them
won) with epoxies. Certainly it deals mostly with organic chemistry.

On behalf of the many excellent microscopists I have known for many years
I disagree violently with the statement that "and most EM biologists (AT
LEAST THE GOOD ONES) understand a lot about organic chemistry."
(Capitalizations are mine). One look at this most valuable of books makes
it clear that most of the good EM biologists have no hope of knowing
enough about polymer chemistry to understand or deal with the varied
problems which can occur with epoxies.

Sections in our laboratory do not "stretch". Unless, of course, I want
them to, for other special reasons. Our standard sections are the same
size as the block face they come from. We deal with keratinized skin
which is no picnic compared other biological tissues.

As usual, I always reserve the right to be wrong.

Hildy Crowley
Sr. Electron Microscopist
Dept of Biol Sciences
University of Denver
Denver, CO

P.S. My book is under lock and key at all times. If it is stolen, I will
have to be hospitalized!



From daemon Thu Mar 30 17:54:55 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Thu, 30 Mar 2000 10:23:23 -0600
Subject: Re: Leaf Microlumina

Contents Retrieved from Microscopy Listserver Archives
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At 08:42 PM 3/29/00 , I wrote:

} This is on ebay now. Here is the URL:
}
} http://cgi.ebay.com/aw-cgi/eBayISAPI.dll?ViewItem&item=296173307
}
} Not announced on ebay, and limited to MSA, I am offering
} an IBM Thinkpad 355 which is optionally available to make this camera truly
} portable. $350 extra gets the Thinkpad. You will still need a Slim SCSI
} PCMCIA card, but these are very low cost. I have one in a Sony VAIO
} and it works great with the Leaf.

It is on ebay and "Now announced on ebay, ..." It is NOT limited to MSA.
Sorry about the poor eye-hand coordination.

gary



From daemon Thu Mar 30 18:21:48 2000



From: mary mckee :      mckee-at-helix.mgh.harvard.edu
Date: Thu, 30 Mar 2000 17:56:15 -0600
Subject: low-temp polymerization of LR white

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Hello, listmembers,

Has anyone got a protocol for embedding LR white in a low-temperature
chamber using UV light? I have a Leica AFS, so setting and maintaining a
temp. is not a problem. I just tried it overnight at -20 C with UV (in
filled gelatin capsules, but without activator) and did not get
polymerization. Any tips would be appreciated.

Mary McKee
Renal Unit
MGH
Charlestown, MA
(617)726-5643




From daemon Thu Mar 30 18:21:48 2000



From: Dr Eric Lachowski :      che136-at-abdn.ac.uk
Date: Thu, 30 Mar 2000 17:56:01 -0600
Subject: pressure systems

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Dear UK CPD users,
In the UK pressure systems which operate at pressures
greater than 0.5 bar are subject to the Pressure Systems
and Transportable Gas Container Regulations 1989. These
rules require, among other things, regular inspection of
the apparatus. You may also find that your institution's
insurers impose a similar rule.

Regards,
Eric

----------------------
Dr Eric Lachowski
Department of Chemistry
University of Aberdeen
Meston Walk
Old Aberdeen AB24 3UE
Scotland
+44 (0)1224 272934 fax 272921
e.lachowski-at-abdn.ac.uk




From daemon Fri Mar 31 08:11:51 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Fri, 31 Mar 2000 08:03:50 -0600
Subject: GIF: angles calibration

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Originally Posted by: shens-at-focus.ruca.ua.ac.be



Hi,

How we can experimentally measure the collection angle when
working with GIF? In other words, how to measure magnification
between the plane of negatives and the plane of GIF entrance? Using
a CCD camera? But normally a CCD camera magnifies an image 20
times of that on a negative, so pictures become uncomparible.

Kind Regards,

Steven




From daemon Fri Mar 31 08:11:51 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Fri, 31 Mar 2000 07:59:42 -0600
Subject: M&M2000: Social Event Information

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Originally posted by: Beverly_E_Maleeff-at-sbphrd.com



The Microscopy & Microanalysis 2000 Local Arrangements Committee
is pleased to announce an additional social event at M&M2000---

TAKE ME OUT TO THE BALL GAME !!

Join us on Tuesday, August 15th as the
PHILADELPHIA PHILLIES meet the
ARIZONA DIAMONDBACKS at Veterans Stadium.
Game time is 7:35 PM.

For $32.50, you'll get:
- a ticket to the game (300 level, on the third base line)
- a $10.00 coupon, good toward purchases at all concession and souvenir stands
- round-trip transportation from the Convention Center to the stadium
on a luxury coach (buses leave at 6:00 PM; return to the convention
center immediately following the game)

A limited number of packages are available. First come, first served.
Maximum 2 packages per person.

Checks, in US$ only, payable to "Microscopy & Microanalysis 2000" can be mailed
to:

Bev Maleeff
Treasurer, M&M2000 LAC
c/o SmithKline Beecham Pharmaceuticals
Mail Code UE 0462
709 Swedeland Road
King of Prussia, PA 19406

Checks only, please. No credit cards accepted.
Your cancelled check will serve as your receipt.

Tickets will be distributed at the M&M2000 Hospitality Booth
on Monday and Tuesday, August 14th & 15th.

Further information about this and other M&M2000 events can be found on our web
site:
http://www.msa.microscopy.com/~mm2000/

See you in August!!

Bev Maleeff
M&M2000 LAC




From daemon Fri Mar 31 08:11:52 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Fri, 31 Mar 2000 08:00:33 -0600
Subject: Job opportunities in Dept of Materials, Oxford, UK

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Originally posted by: ron.doole-at-materials.ox.ac.uk


Hi All,

I enclose details of post doctoral positions currently
available at Dept. of Materials here in Oxford. Please
respond directly to the address given for each post.
Good luck to any applicants,

Ron

----------------------
Mr. R.C. Doole
Department of Materials,
University of Oxford.
Parks Road, Oxford. OX1 3PH. UK.
Phone +44 (0) 1865 273701
Fax +44 (0) 1865 283333
ron.doole-at-materials.ox.ac.uk

**********************************************************************
UNIVERSITY OF OXFORD

Department of Materials

POSTDOCTORAL RESEARCH POSITIONS - Salary range £16,286-£24,479p.a.

Applications are invited for the following positions:

Manufacture of Metal Matrix Composite Components

A position is available for a period of 20 months for a suitably qualified
research assistant to join an inter-disciplinary research team in the
Department of Materials and Department of Engineering Science researching
into the manufacture of next generation aeroengine components from long
fibre reinforced metal matrix composites. This post will study the
fundamentals of fabrication mechanisms, and how these influence subsequent
mechanical performance, using a combination of numerical modelling and
experiments. Candidates should preferably have expertise and ability in one
or more of the following areas: experimental solid mechanics, finite
element analysis of plasticity or metal forming, microstructural
characterisation of materials, composite materials. Please quote DJ00/6

Interface Modification in Organic LED

A position, sponsored by Opsys Limited, is available in the Department of
Materials from April 2000 (or as soon as possible thereafter) for 18 months
in the first instance. The project will explore routes to modifying the
interfaces of the OLEDs with the objective to improve charge transport and
injection. It will involve detailed investigation of the structural and
physical properties of the modified interfaces between organo-lanthanide
phosphors and other organic and inorganic materials. Techniques will
include UPS, SIMS, AFM/STM and electro-optical characterisation of OLEDs.
Expertise in one or more of these is highly desirable. Further details are
available by email from: oleg.salata-at-materials.ox.ac.uk. Please quote
DJ00/7.

Compositional inhomogeneities in information storage materials - effect on
physical properties
(Principal investigators: Amanda Petford-Long and Alfred Cerezo)

A 3-year position funded by the EPSRC, with support from Seagate
Technology, is available in the Department of Materials from May 2000 (or
as soon as possible thereafter). The aim of this project is to address how
local inhomogeneities in the morphology and composition of thin layered
films used for spin-valves and media in information storage technology are
controlled by processing of the materials, and how this in turn determines
their magnetic properties. The project will primarily involve the use of
three-dimensional atom probe (3DAP) analysis to study the chemistry of the
films at near-atomic resolution. Results from 3DAP analysis will be
combined with measurements of magnetic properties to elucidate the role of
the observed nanostructural features on the physical film properties. Some
electron microscopy will also be required for comparative microstructural
analysis. Expertise in materials characterisation is essential, and some
knowledge of thin layered films or magnetic materials would also be
helpful. Please quote ref. DJ00/8.

Applications including a full CV, list of publications and the names and
addresses of three referees should be sent to The Administrator, Department
of Materials, University of Oxford, Parks Road, Oxford, OX1 3PH, from whom
further particulars are available. The closing date for applications is 20
April 2000.




From daemon Fri Mar 31 08:11:52 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Fri, 31 Mar 2000 08:01:46 -0600
Subject: Operator Horror Stories

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Originally posted by: sryazant-at-ucla.edu




It is not "horror" story, but a sort of... Many years ago the colleague
from friendly Lab visited me with great project. The idea was simply and
beautiful. She argues that because the image in the scope (TEM) is green
(green fluorescence of the screen), she wants to modify the sample (I
forgot what, some protein, I believe) by red-fluorescent dye to be able to
see on the screen of the electron microscope the "double-staining": red on
the green background. No comments...

Sergey

} Date: Thu, 30 Mar 2000 09:17:56 -0500
} From: "anderron-at-us.ibm.com"-at-sparc5.microscopy.com
} Subject: Operator Horror Stories
} To: microscopy-at-sparc5.microscopy.com
} Importance: Normal
} X-MIMETrack: Serialize by Router on D01ML065/01/M/IBM(Release 5.0.2b
(Intl)|18
} January 2000) at 30/03/2000 09:18:01
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu
http://www.bol.ucla.edu/~sryazant




From daemon Fri Mar 31 08:11:52 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Fri, 31 Mar 2000 08:02:50 -0600
Subject: CPD testing

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Originally posted by: cgarber-at-2spi.com



-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

As further comment on the pressure testing of the Polaron ® CPD units, I
have been asked to submit the following statement from David Cheshire, a
Manufacturing Manager at VG Microtech, the manufacturers of the Polaron
brand of critical point dryers:
=================================================
We use an independent test facility that has amongst its credentials the
following international approvals : NAMAS, UKAS, NATA. The test
certificates issued by ERA are for each individual chamber and we have
copies of all these. The test itself is a sustained 50% overpressure (2000
psi) for 1 minute. Obviously any leakage would lead to failure and non
certification. In the past units have been overpressured to 2500 and 3000
psi.

I think it is highly unlikely that a "local dive shop" would have the
facilities and wherewithal to support the standards imposed by the various
associations.

Incidentally the window is not quartz but a far denser and tougher material
specifically manufactured for high pressure use.

Best regards
Dave Cheshire

Polaron Range Development Manager
VG Microtech
The Birches Industrial Estate,
Imberhorne Lane,
East Grinstead,
West Sussex.
RH19 1UB.
UK.
===================================================
I believe there was some concern about having this kind of testing, if it
was to be done, in any kind of facility that was not certified according to
the bodies mentioned in David's message.

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.spi.cc
########################
============================================




From daemon Fri Mar 31 08:11:53 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Fri, 31 Mar 2000 07:59:09 -0600
Subject: Administrivia: Oopps.. Nestor broke things

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Colleagues....

In trying to adapt the SPAM filter last night to catch the latest
porn site posting I accidently broke the software. Most messages
got rejected after ~ 5 pm Chicago time. I will try to repost
those messages , however, I may have missed a few. If you
dont' see your posting by the end of the day, please resend it
and accept my apology.

Nestor
Your Friendly Neighborhood SysOp




From daemon Fri Mar 31 08:11:53 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Fri, 31 Mar 2000 08:04:30 -0600
Subject: SEM - Coating

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Originally posted by: bart.depauw-at-rug.ac.be


Hello,

I would like to know why they use Argon in the metal coating devices. At
our laboratory, we don't use Argon, we use air. What is the advantage of
using Argon ?
De Pauw Bart
Faculty of Veterinary Medicine
Morphology
Salisburylaan 133
9820 Merelbeke
Belgium
Phone : 0032(0)9 264.77.19
Fax : 0032(0)9 264.77.90




From daemon Fri Mar 31 08:11:53 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Fri, 31 Mar 2000 08:00:59 -0600
Subject: Ni grids with holey carbon film

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Originally posted by: willem.erasmus-at-sasol.com



Dear fellow microscopists

Does anybody know where I can purchase Ni TEM grids with a holey carbon film
? I can find plenty of copper grids, but the Ni grids seem to be hard to
find.

Thanks
W. Erasmus

Willem Erasmus
Snr. Scientist, Basic Catalysis Research
Sasol Technology
Tel : +27 +16 9603954
Fax : +27 +16 9602826
E-mail : willem.erasmus-at-sasol.com




From daemon Fri Mar 31 08:23:10 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Fri, 31 Mar 2000 08:13:21 -0600
Subject: LM: Help AO Microscope

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Originally Posted by: picomagic-at-canada.com






Email: pink-at-memphis.magibox.net

Name: Bill Cupo

Question: Many years ago the museum bought an AO microscope with an
Expostar Shutter control.
It appears that the control box (model 1190) has been lost.
Is there anyway to buy a replacement?
Does the company still exist? Is the microscope manufactored under another
name?

Any assistance will be greatly appreciated.

Bill Cupo
Exhibits Media Specialist

---------------------------------------------------------------------------




From daemon Fri Mar 31 08:23:10 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Fri, 31 Mar 2000 08:13:59 -0600
Subject: XIth International Congress of Histochemistry and Cytochemistry

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Originally Posted by: george.bou-gharios-at-csc.mrc.ac.uk

I am writing to you in my capacity as secretary general of the XIth
International Congress of Histochemistry and Cytochemistry which is held in
YORK, UK 3-8 July 2000.
The above mentioned Congress is Hosted by the Royal Microscopical Society
(www.rms.org.uk) and n exciting programme has been put together which can
be seen on

www.med.ic.ac.uk/external/ichc_2000/


Dr George Bou-Gharios
Muscle Cell Biology Group
MRC Clinical Sciences Centre
Imperial College School of Medicine
Hammersmith Hospital
Du Cane Road
London W12 0NN
Tel: 0181-383 8261
Fax: 0181- 383 8264
email: george.bou-gharios-at-csc.mrc.ac.uk




From daemon Fri Mar 31 08:23:10 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Fri, 31 Mar 2000 08:14:35 -0600
Subject: IUMAS 2000 Program

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Originally posted by: jfmjfm-at-engin.umich.edu


Hi there folks, just a line to let you know that the schedule of
presentations for the IUMAS 2000 meeting to be held in Kona, Hawaii,
July 9th-14th 2000, is on the Web at:

http://www.microanalysis.org/iumas2000/

Thanks.

Jfm.

--



Dr. John Mansfield CPhys MInstP
North Campus Electron Microbeam Analysis Laboratory
417 SRB, University of Michigan
2455 Hayward, Ann Arbor MI 48109-2143
Phone: (734) 936-3352 FAX (734) 763-2282 Cellular Phone: (734) 358-7555
(Leaving a phone message at 936-3352 is preferable to 358-7555)
Email: jfmjfm-at-engin.umich.edu
URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html
Location: Lat. 42¡ 16' 48" Long. 83¡ 43' 48"




From daemon Fri Mar 31 18:08:43 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Fri, 31 Mar 2000 08:24:27 -0600
Subject: IUMAS 2000 Program

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Originally posted by: jfmjfm-at-engin.umich.edu



Hi there folks, just a line to let you know that the schedule of
presentations for the IUMAS 2000 meeting to be held in Kona, Hawaii,
July 9th-14th 2000, is on the Web at:

http://www.microanalysis.org/iumas2000/

Thanks.

Jfm.

--



Dr. John Mansfield CPhys MInstP
North Campus Electron Microbeam Analysis Laboratory
417 SRB, University of Michigan
2455 Hayward, Ann Arbor MI 48109-2143
Phone: (734) 936-3352 FAX (734) 763-2282 Cellular Phone: (734) 358-7555
(Leaving a phone message at 936-3352 is preferable to 358-7555)
Email: jfmjfm-at-engin.umich.edu
URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html
Location: Lat. 42¡ 16' 48" Long. 83¡ 43' 48"




From daemon Fri Mar 31 18:08:47 2000



From: Christoph Benning :      benning-at-pilot.msu.edu
Date: Fri, 31 Mar 2000 09:46:29 -0500
Subject: TEM-Plant Root Plastids

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Hi there:

I would like to know whether the ratio of plastidic to extraplastidic
membranes changes in roots of Arabidopsis or any other plant in response to
phosphate starvation. Does anyone know of a TEM ultrastructural
morphometric analysis or any other type of analysis towards this end?

Thanks, Christoph Benning

Christoph Benning

Assistant Professor
Dept. of Biochemistry
Michigan State University
East Lansing, MI 48824-1319
USA

Phone: (517) 355-1609
Fax: (517)-353-9334
http://www.bch.msu.edu



From daemon Fri Mar 31 18:08:47 2000



From: Wil Bigelow :      bigelow-at-engin.umich.edu
Date: Fri, 31 Mar 2000 09:57:35 -0400
Subject: RE: Ni grids

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The problem with Ni grids is that Ni is a magnetic metal, and so Ni grids
can give all kinds of trouble. We used to use them back in the early days
when they were about all that was available, and when resolution of EMs
wasn't so great; now-a-days they have lost their popularity.

Wilbur C. Bigelow, Prof. Emeritus
Materials Sci. & Engr., University of Michigan
Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu;
Fx:734-763-4788; Ph:734-662-5237




From daemon Fri Mar 31 18:08:49 2000



From: Barbara Foster :      mme-at-map.com
Date: Fri, 31 Mar 2000 11:02:39 -0800
Subject: Need a non-fluorescing plastic slide

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Hi,

This is a posting for a colleague.

He needs a source of plastic slides and/or film which can be machined and
which do not fluoresce. Anybody have any ideas? Contact me offline.

Many thanks
Barbara Foster
Consortium President
Microscopy/Microscopy Education ...Educating microscopists for greater
productivity.

125 Paridon Street Suite 102 Springfield, MA 01118
PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com
Visit our web site {http://www.MME-Microscopy.com/education}
******************************************************



From daemon Fri Mar 31 18:08:49 2000



From: Barbara Foster :      mme-at-map.com
Date: Fri, 31 Mar 2000 11:10:32 -0800
Subject: Re: LM - mirror objectives available?

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From daemon Fri Mar 31 18:08:49 2000



From: Springett, Margaret J. :      hukee.margaret-at-mayo.edu
Date: Fri, 31 Mar 2000 10:11:18 -0600
Subject: RE: nickel grids

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} ----------
} From: Springett, Margaret J.
} Sent: Friday, March 31, 2000 11:04 AM
} To: 'springett.margaret-at-mayo.edu'
} Subject: nickel grids
}
} I do an extensive amount of immunolabeling, and nickel grids are
} economical,
} but two factors need to be considered on a daily basis. The fact that
} they
} are magnetic can be an advantage as well as a detriment. Their magnetic
} quality allows one to rinse grids floating on a bubble with a magnetic
} stir
} plate, this has been demonstrated in Dr. Bendyan's workshops on
} goldlabeling
} techniques. When trying to retreive a stubborn grid out of a grid box, a
} magnetic tweezer is "heaven sent". Now about magnetic grids in the scope,
} if you stigmate the scope after fine-focusing, your micrograph will be
} focused. I have used these techniques to produce excellent micrographs of
} caveolae at better than 100K magnification. For our use gold grids are
} too
} expensive, so "work around methods" with nickel grids are necessary.
} Marge
}
}
Margaret Springett
IEM Specialist
Electron Microscopy Core Facility
Mayo Foundation and Clinic
Rochester, MN 55905





From daemon Fri Mar 31 18:08:49 2000



From: Barbara Foster :      mme-at-map.com
Date: Fri, 31 Mar 2000 11:10:32 -0800
Subject: Re: LM - mirror objectives available?

Contents Retrieved from Microscopy Listserver Archives
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Frank,

There is an entire FTIR imaging industry out there. SpectraTech has been a
leader in that industry and is a good place to start. Suggest you call Ed
Manke (203-926-8998). Also, John Reffner has been one of the few people
who has really bridged microscopy and spectroscopy. He is the real guru in
this area. John is currently working with SensIR technologies and can be
reached there at 203-207-9700.

The other companies who are in this area come from more of a spectroscopy
background (Jobin Yvon/Instruments SA, Perkin Elmer, Hewlitt Packard, etc.)
and would know less about the microscopy interface.

I taught a microscopy course to the apps specialists at Spectra Tech this
January and had a chance to work with their Continuum, which has a
combination of both glass and reflecting optics on its nosepiece. However,
I think that the highest magnifications they had availalable were only
about 32x objectives. The NAs were really great as I remember, so you
could make use of electronics from the CCD to boost the actual mag to the
screen.

Let me know how you make out.

Best regards,
Barbara Foster
Consortium President
Microscopy/Microscopy Education ...Educating microscopists for greater
productivity.

125 Paridon Street Suite 102 Springfield, MA 01118
PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com
Visit our web site {http://www.MME-Microscopy.com/education}
******************************************************

At 08:12 AM 3/31/00 -0600, Nestor J. Zaluzec wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Fri Mar 31 18:08:50 2000



From: Barbara Foster :      mme-at-map.com
Date: Fri, 31 Mar 2000 11:14:16 -0800
Subject: Re: LM: Help AO Microscope

Contents Retrieved from Microscopy Listserver Archives
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From daemon Fri Mar 31 18:08:50 2000



From: Barbara Foster :      mme-at-map.com
Date: Fri, 31 Mar 2000 11:14:16 -0800
Subject: Re: LM: Help AO Microscope

Contents Retrieved from Microscopy Listserver Archives
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Bill,

AO became part of Cambridge in 1986 then came under the Leica umbrella in
1990. I'd suggest that you start with Leica in Deerfield, IL. Wayne
Buttermore is still there from the "old days" and may have a suggestion:
847-405-7044. The alternative would be to ask around in the used equipment
market. Here are some of my contacts:
MicroOptical Methods Dennis O'Leary Albany, NY 518-482-8200
Spectra Services Mike Specht Rochester, NY 716-654-9500
Vermont Optechs John Oren 802-425-2040
Martin Instruments Bob Martin Easley, SC 864-859-2688

Good luck.
Barbara Foster
Consortium President
Microscopy/Microscopy Education ...Educating microscopists for greater
productivity.

125 Paridon Street Suite 102 Springfield, MA 01118
PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com
Visit our web site {http://www.MME-Microscopy.com/education}
******************************************************




At 08:13 AM 3/31/00 -0600, Nestor J. Zaluzec wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Fri Mar 31 18:08:51 2000



From: William McManus :      billemac-at-biology.usu.edu
Date: Fri, 31 Mar 2000 09:20:25 -0700
Subject: Operator Horror Stories

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We once had a student twist the condenser control knob off a TEM. When asked
to turn the knob clockwise, he just kept turning, until he handed me the
knob.

William McManus
Supervisor
Electron Microscopy Facility
Department of Biology
Utah State University
Logan UT 84322-5305

billEMac-at-cc.usu.edu
435-797-1920


-----Original Message-----
} From: Nestor J. Zaluzec [mailto:zaluzec-at-sparc5.microscopy.com]
Sent: Friday, March 31, 2000 7:02 AM
To: Microscopy-at-sparc5.microscopy.com



Originally posted by: sryazant-at-ucla.edu




It is not "horror" story, but a sort of... Many years ago the colleague
from friendly Lab visited me with great project. The idea was simply and
beautiful. She argues that because the image in the scope (TEM) is green
(green fluorescence of the screen), she wants to modify the sample (I
forgot what, some protein, I believe) by red-fluorescent dye to be able to
see on the screen of the electron microscope the "double-staining": red on
the green background. No comments...

Sergey

} Date: Thu, 30 Mar 2000 09:17:56 -0500
} From: "anderron-at-us.ibm.com"-at-sparc5.microscopy.com
} Subject: Operator Horror Stories
} To: microscopy-at-sparc5.microscopy.com
} Importance: Normal
} X-MIMETrack: Serialize by Router on D01ML065/01/M/IBM(Release 5.0.2b
(Intl)|18
} January 2000) at 30/03/2000 09:18:01
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu
http://www.bol.ucla.edu/~sryazant




From daemon Fri Mar 31 18:08:51 2000



From: W. L. Steffens :      bsteffen-at-arches.uga.edu
Date: Fri, 31 Mar 2000 11:23:36 -0500 (Eastern Standard Time)
Subject: Re: Operator Horror Stories

Contents Retrieved from Microscopy Listserver Archives
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I had one faculty operator several years ago who after a SINGLE lesson
on the Phillips 200, decided to come back late at night and look some
more. After taking some images, he couldn't get the door to the camera
chamber off, since he had failed to vent it. After rummaging through
the lab, he found a large screwdriver and commenced to pry the camera
door off, unconcerned about the marks and gouges that he was putting in
the brass. Needless to say, when the vacuum was finally broken, the
mercury diffusion pump became very unhappy. Eventually we were able to
replace the instrument and the user finally left after being denied
tenure.

W. L. Steffens, Ph.D
Dept. of Pathology
University of Georgia



From daemon Fri Mar 31 18:08:52 2000



From: Larry Allard :      l2a-at-ornl.gov
Date: Fri, 31 Mar 2000 11:43:34 -0500
Subject: Re: Ni grids with holey carbon film

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We get our Ni/holey carbon films from SPI, at http://www.2spi.com/.
They will prepare excellent holey carbon films on just about any
available grid material (for a price of course...).

Larry





} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Dr. Lawrence F. Allard
Senior Research Staff Member
High Temperature Materials Laboratory
Oak Ridge National Laboratory
1 Bethel Valley Road
Bldg. 4515, MS 6064
PO Box 2008
Oak Ridge, TN 37831-6064

423-574-4981
423-574-4913 Fax
l2a-at-ornl.gov



From daemon Fri Mar 31 18:08:53 2000



From: Ladd Research :      ladres-at-worldnet.att.net
Date: Fri, 31 Mar 2000 12:27:46 -0500
Subject: Re: Ni grids with holey carbon film

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Nestor J. Zaluzec wrote:
}
} ------------------------------------------------------------------------} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.}
} Originally posted by: willem.erasmus-at-sasol.com
}
} Dear fellow microscopists
}
} Does anybody know where I can purchase Ni TEM grids with a holey carbon film
} ? I can find plenty of copper grids, but the Ni grids seem to be hard to
} find.
}
} Thanks
} W. Erasmus
}
} Willem Erasmus
} Snr. Scientist, Basic Catalysis Research
} Sasol Technology
} Tel : +27 +16 9603954
} Fax : +27 +16 9602826
} E-mail : willem.erasmus-at-sasol.com

We at Ladd Reseach can supply you with these, as would most of the other
supply houses that make their own holey film. Please let us know what
size mesh you want and a quantity and we can quote you.

Debbie Sicard
Sales Manager

Disclaimer: Ladd Research is a vendor that sells EM supplies.
--

LADD RESEARCH
131 Dorset Lane
Williston, VT 05495 USA

TEL 1-800-451-3406 (US) or 1-802-878-6711 (anywhere)
FAX 1-802-878-8074
e-mail ladres-at-worldnet.att.net
web site http://www.ladd.cc



From daemon Fri Mar 31 18:08:53 2000



From: Larry Allard :      l2a-at-ornl.gov
Date: Fri, 31 Mar 2000 12:08:51 -0500
Subject: Re: Operator Horror Stories

Contents Retrieved from Microscopy Listserver Archives
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} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


The respected PhD I once worked with on our old JEOL JSM-U3 scanner,
who was having trouble making out the details of the image on the
monitor, so grabbed a flashlight and shined it at the screen so he
could see the image a little better... :-).

Larry

}
}
} Originally posted by: sryazant-at-ucla.edu
}
}
}
}
} It is not "horror" story, but a sort of... Many years ago the colleague
} from friendly Lab visited me with great project. The idea was simply and
} beautiful. She argues that because the image in the scope (TEM) is green
} (green fluorescence of the screen), she wants to modify the sample (I
} forgot what, some protein, I believe) by red-fluorescent dye to be able to
} see on the screen of the electron microscope the "double-staining": red on
} the green background. No comments...
}
} Sergey
}
} } Date: Thu, 30 Mar 2000 09:17:56 -0500
} } From: "anderron-at-us.ibm.com"-at-sparc5.microscopy.com
} } Subject: Operator Horror Stories
} } To: microscopy-at-sparc5.microscopy.com
} } Importance: Normal
} } X-MIMETrack: Serialize by Router on D01ML065/01/M/IBM(Release 5.0.2b
} (Intl)|18
} } January 2000) at 30/03/2000 09:18:01
} }
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} }
} } Alan Fox, in a response to the Analytical TEM string, cautioned the
} } question-raiser to beware of people who abuse instruments. Perhaps Nestor
} } will forgive a little microscopy related humor and allow us to start a
} } string on "Operator Horror Stories." Here's ours:
} }
} } The "ham-fisted user" reference made us chuckle/cringe with the memory of
} } a guest "microscopist" in our lab who hauled himself (220 pounds or so) out
} } of his seat by pulling on the half-inserted specimen rod, bending it about
} } 20 degrees or so!
} }
} } Henceforth, when we saw him in the hall (he never came into the scope room
} } again), we referred to him as "Conan the Microscopist"!
} }
} }
} }
} } Ron Anderson, IBM, Hopewell Jct., New York, USA. anderron-at-us.ibm.com
} }
} } IBM Analytical Services; http://www.chips.ibm.com/services/asg
} }
} }
} }
} }
} }
} _____________________________________
}
} Sergey Ryazantsev Ph. D.
} Electron Microscopy
} UCLA School of Medicine
} Department of Biological Chemistry
} Box 951737
} Los Angeles, CA 90095-1737
}
} Phone: (310) 825-1144
} Pager: (310) 845-0248
} FAX (departmental): (310) 206-5272
} mailto:sryazant-at-ucla.edu
} http://www.bol.ucla.edu/~sryazant

Dr. Lawrence F. Allard
Senior Research Staff Member
High Temperature Materials Laboratory
Oak Ridge National Laboratory
1 Bethel Valley Road
Bldg. 4515, MS 6064
PO Box 2008
Oak Ridge, TN 37831-6064

423-574-4981
423-574-4913 Fax
l2a-at-ornl.gov



From daemon Fri Mar 31 18:08:54 2000



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Fri, 31 Mar 2000 13:39:47 -0500
Subject: Carbon coated Ni grids

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-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

W. Erasmus asked:
================================================
Does anybody know where I can purchase Ni TEM grids with a holey carbon film
? I can find plenty of copper grids, but the Ni grids seem to be hard to
find.
=================================================
Some times the coating of Ni grids is a bit more difficult than the coating
of copper grids. This translates to somewhat lower yields and sometimes
higher prices. However, SPI Supplies regularly produces custom coated Ni
grids on whatever mesh and grid the customer specifies and at only a slight
price premium over copper.

Contact me off line for details or visit our webpage URL
http://www.2spi.com/catalog/grids/cusctgrd.html

Some of the other major suppliers of consumables also will coat Ni grids, or
at least that is my understanding.

Chuck
============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.spi.cc
########################
============================================




From daemon Fri Mar 31 18:08:54 2000



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Fri, 31 Mar 2000 08:45:16 -1000 (HST)
Subject: RE: Operator Horror Stories

Contents Retrieved from Microscopy Listserver Archives
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One day a user of our TEM complained that she could see the beam with the
specimen out, but as soon as she put in a grid the screen went
black. After playing with it awhile and ruling out magnetism or something
on the grid holder, I decided that there must be some small magnetic
particle in the stage area that was being pushed around by the grid
holder. Of course the service technician I got by phone wanted me to
begin taking the column apart from the gun on down, but I grew impatient
with this and soon went straight for the stage. I figured I would be
looking for some small metal sliver. Imagine my surprise when I found an
entire plastic pipettortip lying across the bore through the
anticontamination plate in the column! It seems the previous user had
been using these tips as forceps covers, and mysteriously lost one the day
before. It had gone into the column through the airlock with her grid,
not impossible with the Zeiss 10 top-loading system. Although not the
only strange thing I have found in that microscope's column, at about an
inch and a half long it is the largest.

Aloha,
Tina
****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************




From daemon Fri Mar 31 18:08:55 2000



From: Kriho, Virginia :      Vkriho-at-psych.uic.edu
Date: Fri, 31 Mar 2000 12:34:21 -0600
Subject: TEM/peroxidase immunocytochemistry

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am currently using the peroxidase/DAB pre-embedding method for
localization of a novel extracellular matrix protein. I am using 40 micron
vibratome sections. While my results give some extracellular labelling,
most of the labelling is associated with dendritic microtubules. Has anyone
experienced non-specific labelling of microtubules?



From daemon Fri Mar 31 18:08:56 2000



From: MICHAEL DELANNOY :      delannoy-at-mail.jhmi.edu
Date: Fri, 31 Mar 2000 14:48:58 -0500 (EST)
Subject: Core Facility solvency

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Microscpy Core Managers,
Are there any EM/Confocal Microscope facilities within academia that
are running in the black or at least breaking even without a subsidy. If
so what are your rates and overhead, are your salaries included? Also are
there any companies or facilities that do tele-microscopy? All info will be
shared with the listserver, unless confidentiality is requested. Thank You.

Michael Delannoy
Basic Science Microscopy Facility
JHMI



From daemon Fri Mar 31 18:08:58 2000



From: White, Woody N :      Woody.N.White-at-mcdermott.com
Date: Fri, 31 Mar 2000 15:46:00 -0600
Subject: SEM Horror Story

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Many, many years ago... I was asked to evaluate particle size of a powder.
The
powder was dried sludge from a radioactive waste storage tank. My downfall
was
permitting a co-worker to prepare the sample. When I received it, rather
than a
thin layer on carbon tape, it was caked on. Furthermore, the powder turned
out
to be sub-micron in size and almost pure Sr-90! Static and air currents
began
to spread contamination, even before I got it in the chamber. I quickly
left
the area before I became "hot" too.

I did finish the exam... In anti-contamination dress with a full face
respirator at the console!

The job took a few hours. It took two weeks for me and my service engineer
to
disassemble the SEM, clean (and the room) it to reasonable levels and
reassemble. The vacuum system was rather "warm" from that time on...
Nevermore! Nevermore!

Moral: Always insist on making input and final approval if someone else
wants
to prepare your samples.

Woody White
McDermott Technology

My new web URL:
http://woody.white.home.att.net



From daemon Fri Mar 31 18:09:00 2000



From: L R MELSEN :      lmelsen-at-emory.edu
Date: Fri, 31 Mar 2000 16:19:48 -0500
Subject: Storys

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We had a cousin of Conan , who in his addled age, after inserting the
injector tip into the stage of our EM400 could not find his grid in the
microscope; he had dropped it on the floor. Of course the tip must have
fallen off in the stage, so he promptly took a second tip and properly
inserted the second tip on top of the first. Still no grid could be
found. Ah ha, I will leave a polite note explaining the problem. It read
as follows:
" Please check the microscope, I had great difficulty inserting my
specimens last evening."
Philips kindly replaced the bulk of the stage just so they could keep
the original for the museum of what not to do.



From daemon Fri Mar 31 18:09:01 2000



From: Walck, Scott D. :      walck-at-ppg.com
Date: Fri, 31 Mar 2000 16:26:02 -0500
Subject: RE: Operator Horror Stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Two from the University of Florida.

There once were two eager graduate students at Florida in the Materials
Science and Engineering Department (one has the initials MK and now works
for JEOL) that decided that they wanted learn how to align the Philips 300
TEM. Armed with the user manual, they proceeded to align the column, but
couldn't do it. They had to call in the service engineer. The first thing
that the service engineer did was to use a framing square to help to take
the visible bow out of the column. It seems that the user manual they were
working from was different from one for a microscope with a STEM attachment.
(Not witnessed by me, but heard from reliable sources.)

A professor in the same department was escorting a visitor around the EM lab
and the two were discussing possible experiments that might be performed in
the Philips 300. They asked the EM technician to give them a hand opening
up the microscope so they could look inside. The technician was busy and
said that he would be with them in about 5 minutes. Well, busy professors
can't wait for lowly technicians so they decided to open the chamber
themselves. The professor cranked on the handle to open the gun chamber
while it was under vacuum and the voltage was on. Bang, pop, whoosh,
whistles, bells brought the technician running to see what had happened.
After all, they only wanted to look inside. But, there was a happy ending
to the story. The vacuum and diffusion pump were OK and the apertures were
cleaned by the supersonic air. (I was around when this one happened.)

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)



} -----Original Message-----
} From: "anderron-at-us.ibm.com"-at-sparc5.microscopy.com
} [mailto:"anderron-at-us.ibm.com"-at-sparc5.microscopy.com]
} Sent: Thursday, March 30, 2000 9:18 AM
} To: microscopy-at-sparc5.microscopy.com
} Subject: Operator Horror Stories
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.} html
}
}
} --------------------------------------------------------------
} ---------.
}
}
}
} Alan Fox, in a response to the Analytical TEM string, cautioned the
} question-raiser to beware of people who abuse instruments.
} Perhaps Nestor
} will forgive a little microscopy related humor and allow us to start a
} string on "Operator Horror Stories." Here's ours:
}
} The "ham-fisted user" reference made us chuckle/cringe with
} the memory of
} a guest "microscopist" in our lab who hauled himself (220
} pounds or so) out
} of his seat by pulling on the half-inserted specimen rod,
} bending it about
} 20 degrees or so!
}
} Henceforth, when we saw him in the hall (he never came into
} the scope room
} again), we referred to him as "Conan the Microscopist"!
}
}
}
} Ron Anderson, IBM, Hopewell Jct., New York, USA.
} anderron-at-us.ibm.com
}
} IBM Analytical Services; http://www.chips.ibm.com/services/asg
}
}
}
}



From daemon Fri Mar 31 18:09:01 2000



From: john grazul :      grazul-at-physics.bell-labs.com
Date: Fri, 31 Mar 2000 16:48:24 -0500
Subject: Re: Operator Horror Stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


This is my second go around with this, I got the Nestor porno bump this
morning.

I had a student using my JEOL 100 CX II; loading and looking around went
well. When she went to remove the holder she got half way out and yanked it
toward her. This action put a 70 degree bend in the holder. I could never
get the Z height to behave after that for some odd reason.

The other disaster was with my Philips 300, complete with Mercury Dif pump
at that time. I needed to do some anode cleaning, so without deflating the
air table I got on the console finished my work and got off. When the scope
lurched to the right it dumped the contents of the mercury pump into the oil
pump. This sent a cloud of mercury up the column creating an alloy wherever
it went.

Believe it or not the scope is in fine working order with not a trace of
mercury left in in the column. How did I do it? If you can figure it out I
will send the winner a Lucent/Bell Labs brief case...at my cost of course.

John Grazul
Lucent Technologies

"W. L. Steffens" wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} I had one faculty operator several years ago who after a SINGLE lesson
} on the Phillips 200, decided to come back late at night and look some
} more. After taking some images, he couldn't get the door to the camera
} chamber off, since he had failed to vent it. After rummaging through
} the lab, he found a large screwdriver and commenced to pry the camera
} door off, unconcerned about the marks and gouges that he was putting in
} the brass. Needless to say, when the vacuum was finally broken, the
} mercury diffusion pump became very unhappy. Eventually we were able to
} replace the instrument and the user finally left after being denied
} tenure.
}
} W. L. Steffens, Ph.D
} Dept. of Pathology
} University of Georgia



From daemon Fri Mar 31 18:09:03 2000



From: Gordon Couger :      gcouger-at-rfdata.net
Date: Fri, 31 Mar 2000 16:03:27 -0600
Subject: Re: Operator Horror Stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I got in an agument with an engineering prof that disputed
the inverse square law applied to photo flash units.

Gordon

Gordon Couger gcouger-at-couger.com

Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00




From daemon Fri Mar 31 18:09:06 2000



From: Lou Ann Miller :      lamiller-at-ux1.cso.uiuc.edu
Date: Fri, 31 Mar 2000 16:40:27 -0600
Subject: Horror Inspection

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Once, over a decade back, I was working EM and Med Tech at the same
time at a local hospital..

We had a CAP inspection, the officers were from another hospital in the state.


The question I was expected to reason and answer with a straight face was:


* What other protective measures besides standing behind a lead
wall do you take when you put the glass slide into the TEM, to
protect yourself from the flying electrons???



seriously!

I was rather stunned, and started looking from the wall socket to
him and back, but he didn't catch the connection.


I then tried to tactfully explain that vacuum was required, and that
we really didn't need that extra protection!

It was explained to me that he did too know all about EM because he
had a one day workshop.



Lou Ann
{ { { { { { { { { { { { { { { { { {} } } } } } } } } } } } } } } } } } } } } } } } }
Lou Ann Miller
Service Supervisor

Rm 1108 VMBSBld.
College of Veterinary Medicine
Veterinary Biosciences
2001 S. Lincoln Ave
Urbana, IL 61802

Phone: 217-244-1567
Fax: 217-244-1652

lamiller-at-uiuc.edu
lamiller-at-net66.com
turtle_lam-at-yahoo.com

Web Pages:
Lab Page
http://treefrog.cvm.uiuc.edu
Centeral States Microscopy Page
http://treefrog.cvm.uiuc.edu/csmms
Personal Home Page
http://treefrog.cvm.uiuc.edu/lam



From daemon Fri Mar 31 18:09:09 2000



From: David R Hull :      David.R.Hull-at-grc.nasa.gov
Date: Fri, 31 Mar 2000 17:58:37 -0500
Subject: Re: Operator Horror Stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} We once had an operator on our JEOL 840A SEM complain about her
} sample moving. Her sample was samples of vacuum grease she had gold
} coated and was attempting to image in the SEM. When the electron
} beam heated the grease it cracked up the gold coating and charged.
} She was using the SEM to look for silicon in various greases. I
} suggested she use a different technique so we did not end up with
} vaporized grease inside the SEM.

David R. Hull
NASA Glenn Research Center at Lewis Field
Advanced Metallics Branch
Mail Stop 49-1
21000 Brookpark Road
Cleveland, OH 44135

(216) 433-3281
fax (216)977- 7132
david.r.hull-at-grc.nasa.gov



From daemon Fri Mar 31 18:09:12 2000



From: Greg Strout :      gstrout-at-ou.edu
Date: Fri, 31 Mar 2000 16:48:19 -0600
Subject: Re: Operator Horror Stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


It seems everyone has one of these. I had a person who one day
complained of a burning smell while she was at the scope. I went to
investigate and when I arrived in the room there was no smell... burning
or otherwise. Several more times that session she came and got me to
investigate this burning smell in the TEM room. This continued for a
couple of sessions. I did go and investigate because after all, who
wants their TEM on fire? I honestly didn't know what to do about it and
was beginning to seriously question her sanity. Finally she convinced
me to sit with her while she operated the scope and sure enough there
was this faint smell of something burning! I jumped up and tried to
find it, but it had gone away. So...she sat down again and began to
work and the smell, like plastic kitchenware burning in a dishwasher,
came back. Again I jump up and look around....to no avail! I was
curious now. The burning smell only happens when she is sitting at the
scope. Not being a big believer in spontaneous combustion, there had to
be an answer! There was. As part of the TEM course I take the kick
panel off the Zeiss 10 to show the students the guts of the vacuum
system. The kick panel is right below the desk area where you sit up to
the microscope. What she was doing was placing her tennis shoe on the
heater of the diffusion pump and the plastic/rubber would melt and burn
a bit -- just enough to give the room a burning smell. She never did
notice her foot getting warm. When I pointed it out she did admit that
her shoe was warm and had some ugly scars on it to boot! She never
returned after that semester - I guess that she had enough of EM! Now I
carefully point out the hazard and replace the cover quickly when we are
finished with the class.

--
==================================================================
Greg Strout
Electron Microscopist, University of Oklahoma
WWW Virtual Library for Microscopy:
http://www.ou.edu/research/electron/www-vl/
e-mail: gstrout-at-ou.edu
Opinions expressed herein are mine and not necessarily those of
the University of Oklahoma
==================================================================




From daemon Fri Mar 31 18:09:16 2000



From: Ekstrom, Harry :      harry.ekstrom-at-honeywell.com
Date: Fri, 31 Mar 2000 16:35:42 -0700
Subject: SEM Horror Story

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


And all of these cute blunders that happened way back when were caused by
today's scientists/researchers.

Experience, good and bad, is the only way we all really learn.

Enjoying the stories.....

Harry Ekstrom
Honeywell Materials Laboratory



-----Original Message-----
} From: White, Woody N [mailto:Woody.N.White-at-mcdermott.com]
Sent: Friday, March 31, 2000 2:46 PM
To: Microscopy-at-sparc5.microscopy.com



Many, many years ago... I was asked to evaluate particle size of a powder.
The
powder was dried sludge from a radioactive waste storage tank. My downfall
was
permitting a co-worker to prepare the sample. When I received it, rather
than a
thin layer on carbon tape, it was caked on. Furthermore, the powder turned
out
to be sub-micron in size and almost pure Sr-90! Static and air currents
began
to spread contamination, even before I got it in the chamber. I quickly
left
the area before I became "hot" too.

I did finish the exam... In anti-contamination dress with a full face
respirator at the console!

The job took a few hours. It took two weeks for me and my service engineer
to
disassemble the SEM, clean (and the room) it to reasonable levels and
reassemble. The vacuum system was rather "warm" from that time on...
Nevermore! Nevermore!

Moral: Always insist on making input and final approval if someone else
wants
to prepare your samples.

Woody White
McDermott Technology

My new web URL:
http://woody.white.home.att.net



From daemon Fri Mar 31 18:09:16 2000



From: Amy McGough :      amcgough-at-bcm.tmc.edu
Date: Fri, 31 Mar 2000 17:50:10 -0600
Subject: Position Announcement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Position Announcement

Research Scientist in Macromolecular Microscopy

Purdue University Department of Biological Sciences

An opening is available immediately for a scientist specializing in
macromolecular microscopy at Purdue University. The successful
candidate would be responsible for overseeing the day-to-day
operations of the high-resolution electron microscopy facility, training
new users, and assisting outside visitors with data collection and
analysis. He/she would also be expected to work with the cryoEM
groups in the department at planning and implementing the future
directions of the facility, including the development of new
technologies for improved specimen preservation, data collection, and
analysis. The ideal candidate should complement the existing
strengths represented in the department and pursue independent
research funding.

The Department of Biological Sciences at Purdue provides a rich
intellectual environment as well as outstanding modern scientific
facilities including Philips EM420, CM200-FEG, and CM300-FEG
electron cryo-microscopes. Purdue University is home to over 50,000
students, faculty, and staff, and is located in West Lafayette, Indiana -
approximately 1 hour northwest of Indianapolis and 2.5 hours
southeast of Chicago. The area affords the advantages associated with
a major university, while providing an affordable and attractive `small
town' environment in which to live.

Requirements for this position include a Ph.D. in biophysics or one of
the related sciences, a minimum of 5 years post-doctoral work
experience in electron cryo-microscopy of biological molecules, and a
proven ability to work well with others. Preference will be given to
candidates who have successfully developed new technologies and
methodologies for high-resolution electron cryo-microscopy. Salary
will be commensurate with experience and includes full benefits.

Interested individuals should send their resume, relevant reprints, a
statement of research interests and career goals, and provide contact
information (name/phone/e-mail) for three individuals who have
agreed to serve as references to:

Dr. Timothy S. Baker (tsb-at-bragg.bio.purdue.edu)

and/or

Dr. Amy McGough (amcgough-at-bcm.tmc.edu)

Department of Biological Sciences
Lilly Hall of Life Science
Purdue University
West Lafayette, IN 47907-1392

Purdue is an equal access/equal opportunity university.



From daemon Fri Mar 31 18:09:17 2000



From: Paul Webster :      pwebster-at-mailhouse.hei.org
Date: 31 Mar 00 16:04:07 -0800
Subject: Operator Horror Stories

Contents Retrieved from Microscopy Listserver Archives
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------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Is there any truth in the story I was told about a lab in London that replaced the leaded glass on the microscope chamber with regular glass? Apparently the mistake was discovered when all the film on the shelf behind the operator become fogged!

Paul Webster, Ph.D
House Ear Institute
2100 West Third Street
Los Angeles, CA 90057
phone:213 273 8026
fax: 213 413 6739
e-mail: pwebster-at-hei.org
http://www.hei.org/htm/aemi.htm



From daemon Fri Mar 31 18:19:25 2000



From: Jo Dee :      jofish-at-burnham-inst.org
Date: Fri, 31 Mar 2000 16:04:43 -0800
Subject: Re: Operator Horror Stories

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Message-ID: {38E53D1B.C043653A-at-burnham-inst.org}


Hello All,
My two horror stories!
I was working with an investigator who wanted to see matrix vessicles. Well,
we were so happy that we had a nicely fixed sample with "lots" of vessicles.
Nice whole round ones. Well, when the negatives were developed, my director
and I discovered that what we really had were nicely fixed, beautiful
bacteria!!!! No vessicles!
Another time, in school, I was walking down the hallway later in the afternoon
when I saw a scope room door slightly open. I popped my head in to see who was
there and to ask if they wanted the door closed. When I looked inside, I found
a student sound asleep, with hands still holding the knobs of the
microscope!!!!!!!
Funny huh?
Jo

"anderron-at-us.ibm.com"-at-sparc5.microscopy.com wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Alan Fox, in a response to the Analytical TEM string, cautioned the
} question-raiser to beware of people who abuse instruments. Perhaps Nestor
} will forgive a little microscopy related humor and allow us to start a
} string on "Operator Horror Stories." Here's ours:
}
} The "ham-fisted user" reference made us chuckle/cringe with the memory of
} a guest "microscopist" in our lab who hauled himself (220 pounds or so) out
} of his seat by pulling on the half-inserted specimen rod, bending it about
} 20 degrees or so!
}
} Henceforth, when we saw him in the hall (he never came into the scope room
} again), we referred to him as "Conan the Microscopist"!
}
} Ron Anderson, IBM, Hopewell Jct., New York, USA. anderron-at-us.ibm.com
}
} IBM Analytical Services; http://www.chips.ibm.com/services/asg



From daemon Fri Mar 31 18:19:25 2000



From: John Hunt :      hunt-at-ccmr.cornell.edu
Date: Fri, 31 Mar 2000 18:10:58 -0600
Subject: Re: SEM - Coating

Contents Retrieved from Microscopy Listserver Archives
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Bart,
I believe Argon is used because it is a larger molecule and
sputters the metal more efficiently. I also use air with no problem.
Possibly the amount of metal sputtered is slightly less per unit time.
John Hunt

On Fri, 31 Mar 2000, Nestor J. Zaluzec wrote:

} ------------------------------------------------------------------------
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} -----------------------------------------------------------------------.
}
}
}
} Originally posted by: bart.depauw-at-rug.ac.be
}
}
} Hello,
}
} I would like to know why they use Argon in the metal coating devices. At
} our laboratory, we don't use Argon, we use air. What is the advantage of
} using Argon ?
} De Pauw Bart
} Faculty of Veterinary Medicine
} Morphology
} Salisburylaan 133
} 9820 Merelbeke
} Belgium
} Phone : 0032(0)9 264.77.19
} Fax : 0032(0)9 264.77.90
}
}
}




From daemon Fri Mar 31 18:19:26 2000



From: pe13-at-cam.ac.uk
Date: Fri, 31 Mar 2000 18:11:27 -0600
Subject: Re: SEM - Coating

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Air contains water vapour, oxygen and carbon dioxide all of which can be
broken down in the plasma generated in a sputter coater and gve rise to
nasty active species which can damage delicate samples. Argon is used
because of its relative high atomic number and this ensures there is
multiple scattering of the sputtered metal. There is a Zenon sputter coater
on the market which should sputter even better but I have no experience
with it. Come to the Lehigh Short Course(contact {slc6-at-lehigh.edu) and we
will tell you all about it.

Patrick Echlin
Cambridge




From daemon Fri Mar 31 18:19:26 2000



From: Debby Sherman :      sherman-at-btny.purdue.edu
Date: Fri, 31 Mar 2000 18:12:12 -0600
Subject: Re: Operator Horror Stories

Contents Retrieved from Microscopy Listserver Archives
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Many years ago we had a Siemens EM-101. This was a relatively new
microscope and beautifully machined with German precision. The camera
chamber was a work of art. Each film plate (we used glass back then) was
encased in it's own light-tight cassette. The camera would move a film
cassette into position for exposure and pull off the cassette cover. The
operator would then expose the film and move the casette into a drop box.
The cassette cover would be pushed closed in the process.
This camera worked almost flawlessly. On occassion, an operator, when
loading the camera, would put the cassettes in on an angle. When you went
to take the first image, the cassette would jam and not move into position.
All that was necessary to unjam was to break vacuum, giggle the cassette
with your hand to straighten it and then repump the camera chamber and get
back to work...a 10 minute process as most.
We had one operator who thought she knew it all. She was working on the
microscope one evening and the camera jammed. Her solution was to take out
the camera and take it apart...screw by screw and spring by spring. She
ended up with an incredible heap of parts and of course had no clue as to
how to put it back together. Neither did the Siemens service engineers.
They had never seen one apart! They had to find another camera (not easy
in those days with relatively few of these instruments around), take it
apart..carefully marking where each piece came from...and then reassembled
each camera simultaneously. It took hours!!
Moral of the story...NO ONE fixed ANYTHING without permission of the lab
personnel!!



Debby Sherman, Manager Phone: 765-494-6666
Microscopy Center in Agriculture FAX: 765-494-5896
Dept. of Botany & Plant Pathology E-mail: sherman-at-btny.purdue.edu
Purdue University
1057 Whistler Building
West Lafayette, IN 47907-1057




From daemon Sat Apr 01 07:39:47 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 31 Mar 2000 19:37:29 -0600
Subject: Sputter coater availability before a new one

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am preparing to purchase a sputter coater for Au/Pd SEM
specimen preparation. I have narrowed the search to the VG-Scientific
Polaron SC7620. My old Polaron E5100 is very tired.

I have located used Hummer VI, Fullam EMS-76M and Denton
Desk II coaters for about $1000 less than new. Some of these
include the pump. However, I have several dual stage mech
pumps, so this is not an issue.

If anyone knows of a source for a good/recent used coater,
I would appreciate knowing about it. Otherwise, I will go with
a new one.

tnx,
gary g.



From daemon Sat Apr 01 07:39:52 2000



From: Damian :      dneuberger-at-mindspring.com
Date: Fri, 31 Mar 2000 22:57:16 -0600
Subject: RE: SEM Horror Story

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


These are great stories and would make a wonderful collection, especially
to hand out to students of EM. Yes, experience is a great way to learn but
it's often best, and cheaper, to learn from some else's.

Damian Neuberger



From daemon Sat Apr 01 07:39:53 2000



From: Damian :      dneuberger-at-mindspring.com
Date: Fri, 31 Mar 2000 23:10:28 -0600
Subject: Re: Operator Horror Stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Paul,

I heard that one years ago. Also one that supposedly happened at Univ.
Wisconsin. Someone apparently cleaned a scope's internal parts in acetone
and started pumping. Before it was fully pumped down, they turned on the
filament. Bang! Nice story but could it possibly happen? I would think
that any acetone residue would evaporate very quickly or is there some
other phenomenon here relating to ratio of gases?

Damian Neuberger, Ph.D.
Baxter Healthcare, Inc.
WG3-2S
P.O. Box 490
Round Lake, IL 60073
e-mail: damian_neuberger-at-baxter.co,
Tel: 847-270-5888
Fax: 847-270-5897

} Is there any truth in the story I was told about a lab in London that
} replaced the leaded glass on the microscope chamber with regular
} glass? Apparently the mistake was discovered when all the film on the
} shelf behind the operator become fogged!
}
} Paul Webster, Ph.D
} House Ear Institute
} 2100 West Third Street
} Los Angeles, CA 90057
} phone:213 273 8026
} fax: 213 413 6739
} e-mail: pwebster-at-hei.org
} http://www.hei.org/htm/aemi.htm



From daemon Sat Apr 01 07:40:09 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 31 Mar 2000 19:37:29 -0600
Subject: Sputter coater availability before a new one

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am preparing to purchase a sputter coater for Au/Pd SEM
specimen preparation. I have narrowed the search to the VG-Scientific
Polaron SC7620. My old Polaron E5100 is very tired.

I have located used Hummer VI, Fullam EMS-76M and Denton
Desk II coaters for about $1000 less than new. Some of these
include the pump. However, I have several dual stage mech
pumps, so this is not an issue.

If anyone knows of a source for a good/recent used coater,
I would appreciate knowing about it. Otherwise, I will go with
a new one.

tnx,
gary g.




From daemon Sat Apr 01 07:40:13 2000



From: Damian :      dneuberger-at-mindspring.com
Date: Fri, 31 Mar 2000 22:57:16 -0600
Subject: RE: SEM Horror Story

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


These are great stories and would make a wonderful collection, especially
to hand out to students of EM. Yes, experience is a great way to learn but
it's often best, and cheaper, to learn from some else's.

Damian Neuberger




From daemon Sat Apr 01 07:40:15 2000



From: Damian :      dneuberger-at-mindspring.com
Date: Fri, 31 Mar 2000 23:10:28 -0600
Subject: Re: Operator Horror Stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Paul,

I heard that one years ago. Also one that supposedly happened at Univ.
Wisconsin. Someone apparently cleaned a scope's internal parts in acetone
and started pumping. Before it was fully pumped down, they turned on the
filament. Bang! Nice story but could it possibly happen? I would think
that any acetone residue would evaporate very quickly or is there some
other phenomenon here relating to ratio of gases?

Damian Neuberger, Ph.D.
Baxter Healthcare, Inc.
WG3-2S
P.O. Box 490
Round Lake, IL 60073
e-mail: damian_neuberger-at-baxter.co,
Tel: 847-270-5888
Fax: 847-270-5897

} Is there any truth in the story I was told about a lab in London that
} replaced the leaded glass on the microscope chamber with regular
} glass? Apparently the mistake was discovered when all the film on the
} shelf behind the operator become fogged!
}
} Paul Webster, Ph.D
} House Ear Institute
} 2100 West Third Street
} Los Angeles, CA 90057
} phone:213 273 8026
} fax: 213 413 6739
} e-mail: pwebster-at-hei.org
} http://www.hei.org/htm/aemi.htm




From daemon Sat Apr 01 07:40:20 2000



From: Lou Solebello :      microls1297-at-mindspring.com
Date: Friday, March 31, 2000 9:26 PM
Subject: Storys

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


My story is not as much of a horror as it is embarassing. Several years
back I received a package of samples from a regular client without any paper
work describing what the samples were. This is not unusual since we
frequently recieve unknowns. I proceeded to open the package and was
abruptly assaulted with an extremely strong odor of bannanas. Apparantly one
of the samples was a vial of concentrated bannana flavoring. It was months
(almost a year) before the odor completely disappeared from my office, and I
was cajoled about it frequently. I by the way, hate bannanas.............

Lou Solebello
-----Original Message-----
} From: L R MELSEN {lmelsen-at-emory.edu}
To: Microscopy-at-sparc5.microscopy.com {Microscopy-at-sparc5.microscopy.com}



From daemon Sat Apr 01 14:14:08 2000



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Sat, 01 Apr 2000 10:13:34 -0500
Subject: Microscope nightmares

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Here is a "just off the press" example (but this does come up at least once
a year):

I fielded a phone call from a distraught SEM lab manager who told me that
some months ago he put Dow Corning fluid into his diffusion pump "to save
money". And now that he has had an accident, the silicone fluid of course,
has contaminated his system, so he was asking us, "what organic solvent will
easily remove it."

He was especially upset when I told him that his EDS detector will see Si
everywhere also, because they do a lot of analyses for Si!

I won't repeat what he told me when I tried to explain the reality of his
situation......

But it does go to show that there are a lot of new people entering our
profession, some with less training and experience with vacuum than others,
so such stories are very well worth repeating.

But just out of curiosity, is there some "recommended procedure" for
removing silicone fluid from the internal parts of a column, and also
removing it from the window of an EDS detector? I presume one can always
call in an outside service provider with experienced people but a lot of
users out there just don't have the budget for something like that. But
they do have a good supply of student manpower.

While we are on the subject of silicone, a few weeks ago a well known TEM
user got me on the phone to say "hello" and commented that he had just
placed an order for silicone grease and yes, he said, he was going to be
using it on the o rings of his column. I told him I thought that he should
be using other greases and his response was "don't listen to dogma, I
thought you read the listserver!". Am I correct, namely that one should not
be using silicone grease on the o rings of a column instrument?

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.spi.cc
########################
============================================




From daemon Sat Apr 01 14:14:09 2000



From: Frederick Schamber :      fhscham-at-sgi.net
Date: Sat, 01 Apr 2000 12:22:10 -0500
Subject: Horror Stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am enjoying the "horror stories" (don't we all enjoy hearing about
someone else's stupid mistake) and thought I would share one from the
"software" category.

I had just joined Northern Scientific (later Tracor Northern, now Noran)
and was writing EDS software for our first computerized analyzer (the
venerable NS-880) -- the year was 1972, and my software, like any of
the stuff being produced in those years where we wrote software on paper
tape for machines with 4K memories, just wasn't all that "user
friendly", so it was not unusual to have to walk people through
difficult command sequences. Then too, computers were pretty new to
everyone and people sometimes made silly-sounding mistakes out of pure
inexperience (one highly intelligent customer, when instructed to 'type
a space', dutifully typed "A SPACE" on the keyboard). One particular
customer, however, defied all attempts at instruction and had passed
well beyond "complaining" into the realm of "abusive" and "insulting".
He insisted that he was following the instructions to the letter, but
that our miserable software consistently malfunctioned. We were unable
to duplicate the problem and finally several of us drove a considerable
distance to his lab to get to the bottom of this. We went through the
operations with him watching intently and the software performed
flawlessly, which he acknowledged. We then asked him to operate the
software himself and midway through, observed that he was responding to
one of the dialog questions with a decidedly inappropriate response. We
had him back out of this and repeat it with the correct response and the
software then ran perfectly. To which his response was to draw himself
up and state with great authority and indignation: "but when you run it
THE WAY I DO, it doesn't work!"

Fred Schamber
RJ Lee Instruments Limited



From daemon Sat Apr 01 14:14:09 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Fri, 31 Mar 2000 08:13:21 -0600
Subject: LM: Help AO Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Email: pink-at-memphis.magibox.net

Name: Bill Cupo

Question: Many years ago the museum bought an AO microscope with an
Expostar Shutter control.
It appears that the control box (model 1190) has been lost.
Is there anyway to buy a replacement?
Does the company still exist? Is the microscope manufactored under another
name?

Any assistance will be greatly appreciated.

Bill Cupo
Exhibits Media Specialist

---------------------------------------------------------------------------





From daemon Sat Apr 01 14:14:10 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Fri, 31 Mar 2000 08:01:46 -0600
Subject: Operator Horror Stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Originally posted by: sryazant-at-ucla.edu




It is not "horror" story, but a sort of... Many years ago the colleague
from friendly Lab visited me with great project. The idea was simply and
beautiful. She argues that because the image in the scope (TEM) is green
(green fluorescence of the screen), she wants to modify the sample (I
forgot what, some protein, I believe) by red-fluorescent dye to be able to
see on the screen of the electron microscope the "double-staining": red on
the green background. No comments...

Sergey

} Date: Thu, 30 Mar 2000 09:17:56 -0500
} From: "anderron-at-us.ibm.com"-at-sparc5.microscopy.com
} Subject: Operator Horror Stories
} To: microscopy-at-sparc5.microscopy.com
} Importance: Normal
} X-MIMETrack: Serialize by Router on D01ML065/01/M/IBM(Release 5.0.2b
(Intl)|18
} January 2000) at 30/03/2000 09:18:01
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu
http://www.bol.ucla.edu/~sryazant





From daemon Sat Apr 01 14:14:10 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Fri, 31 Mar 2000 08:13:21 -0600
Subject: LM: Help AO Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Email: pink-at-memphis.magibox.net

Name: Bill Cupo

Question: Many years ago the museum bought an AO microscope with an
Expostar Shutter control.
It appears that the control box (model 1190) has been lost.
Is there anyway to buy a replacement?
Does the company still exist? Is the microscope manufactored under another
name?

Any assistance will be greatly appreciated.

Bill Cupo
Exhibits Media Specialist

---------------------------------------------------------------------------





From daemon Sat Apr 01 14:14:10 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Fri, 31 Mar 2000 08:13:59 -0600
Subject: XIth International Congress of Histochemistry and Cytochemistry

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Originally Posted by: george.bou-gharios-at-csc.mrc.ac.uk

I am writing to you in my capacity as secretary general of the XIth
International Congress of Histochemistry and Cytochemistry which is held in
YORK, UK 3-8 July 2000.
The above mentioned Congress is Hosted by the Royal Microscopical Society
(www.rms.org.uk) and n exciting programme has been put together which can
be seen on

www.med.ic.ac.uk/external/ichc_2000/


Dr George Bou-Gharios
Muscle Cell Biology Group
MRC Clinical Sciences Centre
Imperial College School of Medicine
Hammersmith Hospital
Du Cane Road
London W12 0NN
Tel: 0181-383 8261
Fax: 0181- 383 8264
email: george.bou-gharios-at-csc.mrc.ac.uk





From daemon Sat Apr 01 14:14:11 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Fri, 31 Mar 2000 08:03:50 -0600
Subject: GIF: angles calibration

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Originally Posted by: picomagic-at-canada.com






Originally Posted by: shens-at-focus.ruca.ua.ac.be



Hi,

How we can experimentally measure the collection angle when
working with GIF? In other words, how to measure magnification
between the plane of negatives and the plane of GIF entrance? Using
a CCD camera? But normally a CCD camera magnifies an image 20
times of that on a negative, so pictures become uncomparible.

Kind Regards,

Steven





From daemon Sat Apr 01 14:14:11 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Fri, 31 Mar 2000 08:13:59 -0600
Subject: XIth International Congress of Histochemistry and Cytochemistry

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Originally Posted by: george.bou-gharios-at-csc.mrc.ac.uk

I am writing to you in my capacity as secretary general of the XIth
International Congress of Histochemistry and Cytochemistry which is held in
YORK, UK 3-8 July 2000.
The above mentioned Congress is Hosted by the Royal Microscopical Society
(www.rms.org.uk) and n exciting programme has been put together which can
be seen on

www.med.ic.ac.uk/external/ichc_2000/


Dr George Bou-Gharios
Muscle Cell Biology Group
MRC Clinical Sciences Centre
Imperial College School of Medicine
Hammersmith Hospital
Du Cane Road
London W12 0NN
Tel: 0181-383 8261
Fax: 0181- 383 8264
email: george.bou-gharios-at-csc.mrc.ac.uk





From daemon Sat Apr 01 14:14:11 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Fri, 31 Mar 2000 07:59:09 -0600
Subject: Administrivia: Oopps.. Nestor broke things

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Originally Posted by: picomagic-at-canada.com





Colleagues....

In trying to adapt the SPAM filter last night to catch the latest
porn site posting I accidently broke the software. Most messages
got rejected after ~ 5 pm Chicago time. I will try to repost
those messages , however, I may have missed a few. If you
dont' see your posting by the end of the day, please resend it
and accept my apology.

Nestor
Your Friendly Neighborhood SysOp





From daemon Sat Apr 01 14:14:12 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Fri, 31 Mar 2000 07:59:09 -0600
Subject: Administrivia: Oopps.. Nestor broke things

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Colleagues....

In trying to adapt the SPAM filter last night to catch the latest
porn site posting I accidently broke the software. Most messages
got rejected after ~ 5 pm Chicago time. I will try to repost
those messages , however, I may have missed a few. If you
dont' see your posting by the end of the day, please resend it
and accept my apology.

Nestor
Your Friendly Neighborhood SysOp





From daemon Sat Apr 01 14:14:12 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Fri, 31 Mar 2000 08:03:50 -0600
Subject: GIF: angles calibration

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Originally Posted by: shens-at-focus.ruca.ua.ac.be



Hi,

How we can experimentally measure the collection angle when
working with GIF? In other words, how to measure magnification
between the plane of negatives and the plane of GIF entrance? Using
a CCD camera? But normally a CCD camera magnifies an image 20
times of that on a negative, so pictures become uncomparible.

Kind Regards,

Steven





From daemon Sat Apr 01 14:14:13 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Fri, 31 Mar 2000 08:01:46 -0600
Subject: Operator Horror Stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Originally posted by: sryazant-at-ucla.edu




It is not "horror" story, but a sort of... Many years ago the colleague
from friendly Lab visited me with great project. The idea was simply and
beautiful. She argues that because the image in the scope (TEM) is green
(green fluorescence of the screen), she wants to modify the sample (I
forgot what, some protein, I believe) by red-fluorescent dye to be able to
see on the screen of the electron microscope the "double-staining": red on
the green background. No comments...

Sergey

} Date: Thu, 30 Mar 2000 09:17:56 -0500
} From: "anderron-at-us.ibm.com"-at-sparc5.microscopy.com
} Subject: Operator Horror Stories
} To: microscopy-at-sparc5.microscopy.com
} Importance: Normal
} X-MIMETrack: Serialize by Router on D01ML065/01/M/IBM(Release 5.0.2b
(Intl)|18
} January 2000) at 30/03/2000 09:18:01
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu
http://www.bol.ucla.edu/~sryazant





From daemon Sat Apr 01 14:14:13 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Fri, 31 Mar 2000 08:04:30 -0600
Subject: SEM - Coating

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Originally posted by: bart.depauw-at-rug.ac.be


Hello,

I would like to know why they use Argon in the metal coating devices. At
our laboratory, we don't use Argon, we use air. What is the advantage of
using Argon ?
De Pauw Bart
Faculty of Veterinary Medicine
Morphology
Salisburylaan 133
9820 Merelbeke
Belgium
Phone : 0032(0)9 264.77.19
Fax : 0032(0)9 264.77.90





From daemon Sat Apr 01 14:14:14 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Fri, 31 Mar 2000 08:04:30 -0600
Subject: SEM - Coating

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Originally posted by: bart.depauw-at-rug.ac.be


Hello,

I would like to know why they use Argon in the metal coating devices. At
our laboratory, we don't use Argon, we use air. What is the advantage of
using Argon ?
De Pauw Bart
Faculty of Veterinary Medicine
Morphology
Salisburylaan 133
9820 Merelbeke
Belgium
Phone : 0032(0)9 264.77.19
Fax : 0032(0)9 264.77.90





From daemon Sat Apr 01 14:14:14 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Fri, 31 Mar 2000 08:02:50 -0600
Subject: CPD testing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Originally posted by: cgarber-at-2spi.com



-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

As further comment on the pressure testing of the Polaron ® CPD units, I
have been asked to submit the following statement from David Cheshire, a
Manufacturing Manager at VG Microtech, the manufacturers of the Polaron
brand of critical point dryers:
=================================================
We use an independent test facility that has amongst its credentials the
following international approvals : NAMAS, UKAS, NATA. The test
certificates issued by ERA are for each individual chamber and we have
copies of all these. The test itself is a sustained 50% overpressure (2000
psi) for 1 minute. Obviously any leakage would lead to failure and non
certification. In the past units have been overpressured to 2500 and 3000
psi.

I think it is highly unlikely that a "local dive shop" would have the
facilities and wherewithal to support the standards imposed by the various
associations.

Incidentally the window is not quartz but a far denser and tougher material
specifically manufactured for high pressure use.

Best regards
Dave Cheshire

Polaron Range Development Manager
VG Microtech
The Birches Industrial Estate,
Imberhorne Lane,
East Grinstead,
West Sussex.
RH19 1UB.
UK.
===================================================
I believe there was some concern about having this kind of testing, if it
was to be done, in any kind of facility that was not certified according to
the bodies mentioned in David's message.

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.spi.cc
########################
============================================





From daemon Sat Apr 01 14:14:15 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Fri, 31 Mar 2000 08:02:50 -0600
Subject: CPD testing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Originally posted by: cgarber-at-2spi.com



-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

As further comment on the pressure testing of the Polaron ® CPD units, I
have been asked to submit the following statement from David Cheshire, a
Manufacturing Manager at VG Microtech, the manufacturers of the Polaron
brand of critical point dryers:
=================================================
We use an independent test facility that has amongst its credentials the
following international approvals : NAMAS, UKAS, NATA. The test
certificates issued by ERA are for each individual chamber and we have
copies of all these. The test itself is a sustained 50% overpressure (2000
psi) for 1 minute. Obviously any leakage would lead to failure and non
certification. In the past units have been overpressured to 2500 and 3000
psi.

I think it is highly unlikely that a "local dive shop" would have the
facilities and wherewithal to support the standards imposed by the various
associations.

Incidentally the window is not quartz but a far denser and tougher material
specifically manufactured for high pressure use.

Best regards
Dave Cheshire

Polaron Range Development Manager
VG Microtech
The Birches Industrial Estate,
Imberhorne Lane,
East Grinstead,
West Sussex.
RH19 1UB.
UK.
===================================================
I believe there was some concern about having this kind of testing, if it
was to be done, in any kind of facility that was not certified according to
the bodies mentioned in David's message.

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.spi.cc
########################
============================================





From daemon Sat Apr 01 14:14:15 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Fri, 31 Mar 2000 08:24:27 -0600
Subject: IUMAS 2000 Program

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Originally posted by: jfmjfm-at-engin.umich.edu



Hi there folks, just a line to let you know that the schedule of
presentations for the IUMAS 2000 meeting to be held in Kona, Hawaii,
July 9th-14th 2000, is on the Web at:

http://www.microanalysis.org/iumas2000/

Thanks.

Jfm.

--



Dr. John Mansfield CPhys MInstP
North Campus Electron Microbeam Analysis Laboratory
417 SRB, University of Michigan
2455 Hayward, Ann Arbor MI 48109-2143
Phone: (734) 936-3352 FAX (734) 763-2282 Cellular Phone: (734) 358-7555
(Leaving a phone message at 936-3352 is preferable to 358-7555)
Email: jfmjfm-at-engin.umich.edu
URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html
Location: Lat. 42¡ 16' 48" Long. 83¡ 43' 48"





From daemon Sat Apr 01 14:14:15 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Fri, 31 Mar 2000 08:24:27 -0600
Subject: IUMAS 2000 Program

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Originally posted by: jfmjfm-at-engin.umich.edu



Hi there folks, just a line to let you know that the schedule of
presentations for the IUMAS 2000 meeting to be held in Kona, Hawaii,
July 9th-14th 2000, is on the Web at:

http://www.microanalysis.org/iumas2000/

Thanks.

Jfm.

--



Dr. John Mansfield CPhys MInstP
North Campus Electron Microbeam Analysis Laboratory
417 SRB, University of Michigan
2455 Hayward, Ann Arbor MI 48109-2143
Phone: (734) 936-3352 FAX (734) 763-2282 Cellular Phone: (734) 358-7555
(Leaving a phone message at 936-3352 is preferable to 358-7555)
Email: jfmjfm-at-engin.umich.edu
URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html
Location: Lat. 42¡ 16' 48" Long. 83¡ 43' 48"





From daemon Sat Apr 01 14:14:16 2000



From: Wil Bigelow :      bigelow-at-engin.umich.edu
Date: Fri, 31 Mar 2000 09:57:35 -0400
Subject: RE: Ni grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The problem with Ni grids is that Ni is a magnetic metal, and so Ni grids
can give all kinds of trouble. We used to use them back in the early days
when they were about all that was available, and when resolution of EMs
wasn't so great; now-a-days they have lost their popularity.

Wilbur C. Bigelow, Prof. Emeritus
Materials Sci. & Engr., University of Michigan
Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu;
Fx:734-763-4788; Ph:734-662-5237





From daemon Sat Apr 01 14:14:16 2000



From: Wil Bigelow :      bigelow-at-engin.umich.edu
Date: Fri, 31 Mar 2000 09:57:35 -0400
Subject: RE: Ni grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The problem with Ni grids is that Ni is a magnetic metal, and so Ni grids
can give all kinds of trouble. We used to use them back in the early days
when they were about all that was available, and when resolution of EMs
wasn't so great; now-a-days they have lost their popularity.

Wilbur C. Bigelow, Prof. Emeritus
Materials Sci. & Engr., University of Michigan
Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu;
Fx:734-763-4788; Ph:734-662-5237





From daemon Sat Apr 01 14:14:16 2000



From: Christoph Benning :      benning-at-pilot.msu.edu
Date: Fri, 31 Mar 2000 09:46:29 -0500
Subject: TEM-Plant Root Plastids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi there:

I would like to know whether the ratio of plastidic to extraplastidic
membranes changes in roots of Arabidopsis or any other plant in response to
phosphate starvation. Does anyone know of a TEM ultrastructural
morphometric analysis or any other type of analysis towards this end?

Thanks, Christoph Benning

Christoph Benning

Assistant Professor
Dept. of Biochemistry
Michigan State University
East Lansing, MI 48824-1319
USA

Phone: (517) 355-1609
Fax: (517)-353-9334
http://www.bch.msu.edu




From daemon Sat Apr 01 14:14:17 2000



From: Christoph Benning :      benning-at-pilot.msu.edu
Date: Fri, 31 Mar 2000 09:46:29 -0500
Subject: TEM-Plant Root Plastids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi there:

I would like to know whether the ratio of plastidic to extraplastidic
membranes changes in roots of Arabidopsis or any other plant in response to
phosphate starvation. Does anyone know of a TEM ultrastructural
morphometric analysis or any other type of analysis towards this end?

Thanks, Christoph Benning

Christoph Benning

Assistant Professor
Dept. of Biochemistry
Michigan State University
East Lansing, MI 48824-1319
USA

Phone: (517) 355-1609
Fax: (517)-353-9334
http://www.bch.msu.edu




From daemon Sat Apr 01 14:14:19 2000



From: W. L. Steffens :      bsteffen-at-arches.uga.edu
Date: Fri, 31 Mar 2000 11:23:36 -0500 (Eastern Standard Time)
Subject: Re: Operator Horror Stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I had one faculty operator several years ago who after a SINGLE lesson
on the Phillips 200, decided to come back late at night and look some
more. After taking some images, he couldn't get the door to the camera
chamber off, since he had failed to vent it. After rummaging through
the lab, he found a large screwdriver and commenced to pry the camera
door off, unconcerned about the marks and gouges that he was putting in
the brass. Needless to say, when the vacuum was finally broken, the
mercury diffusion pump became very unhappy. Eventually we were able to
replace the instrument and the user finally left after being denied
tenure.

W. L. Steffens, Ph.D
Dept. of Pathology
University of Georgia




From daemon Sat Apr 01 14:14:19 2000



From: Barbara Foster :      mme-at-map.com
Date: Fri, 31 Mar 2000 11:02:39 -0800
Subject: Need a non-fluorescing plastic slide

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

This is a posting for a colleague.

He needs a source of plastic slides and/or film which can be machined and
which do not fluoresce. Anybody have any ideas? Contact me offline.

Many thanks
Barbara Foster
Consortium President
Microscopy/Microscopy Education ...Educating microscopists for greater
productivity.

125 Paridon Street Suite 102 Springfield, MA 01118
PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com
Visit our web site {http://www.MME-Microscopy.com/education}
******************************************************



From daemon Sat Apr 01 14:14:20 2000



From: Barbara Foster :      mme-at-map.com
Date: Fri, 31 Mar 2000 11:02:39 -0800
Subject: Need a non-fluorescing plastic slide

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

This is a posting for a colleague.

He needs a source of plastic slides and/or film which can be machined and
which do not fluoresce. Anybody have any ideas? Contact me offline.

Many thanks
Barbara Foster
Consortium President
Microscopy/Microscopy Education ...Educating microscopists for greater
productivity.

125 Paridon Street Suite 102 Springfield, MA 01118
PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com
Visit our web site {http://www.MME-Microscopy.com/education}
******************************************************



From daemon Sat Apr 01 14:14:20 2000



From: W. L. Steffens :      bsteffen-at-arches.uga.edu
Date: Fri, 31 Mar 2000 11:23:36 -0500 (Eastern Standard Time)
Subject: Re: Operator Horror Stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I had one faculty operator several years ago who after a SINGLE lesson
on the Phillips 200, decided to come back late at night and look some
more. After taking some images, he couldn't get the door to the camera
chamber off, since he had failed to vent it. After rummaging through
the lab, he found a large screwdriver and commenced to pry the camera
door off, unconcerned about the marks and gouges that he was putting in
the brass. Needless to say, when the vacuum was finally broken, the
mercury diffusion pump became very unhappy. Eventually we were able to
replace the instrument and the user finally left after being denied
tenure.

W. L. Steffens, Ph.D
Dept. of Pathology
University of Georgia




From daemon Sat Apr 01 14:14:21 2000



From: Springett, Margaret J. :      hukee.margaret-at-mayo.edu
Date: Fri, 31 Mar 2000 10:11:18 -0600
Subject: RE: nickel grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




} ----------
} From: Springett, Margaret J.
} Sent: Friday, March 31, 2000 11:04 AM
} To: 'springett.margaret-at-mayo.edu'
} Subject: nickel grids
}
} I do an extensive amount of immunolabeling, and nickel grids are
} economical,
} but two factors need to be considered on a daily basis. The fact that
} they
} are magnetic can be an advantage as well as a detriment. Their magnetic
} quality allows one to rinse grids floating on a bubble with a magnetic
} stir
} plate, this has been demonstrated in Dr. Bendyan's workshops on
} goldlabeling
} techniques. When trying to retreive a stubborn grid out of a grid box, a
} magnetic tweezer is "heaven sent". Now about magnetic grids in the scope,
} if you stigmate the scope after fine-focusing, your micrograph will be
} focused. I have used these techniques to produce excellent micrographs of
} caveolae at better than 100K magnification. For our use gold grids are
} too
} expensive, so "work around methods" with nickel grids are necessary.
} Marge
}
}
Margaret Springett
IEM Specialist
Electron Microscopy Core Facility
Mayo Foundation and Clinic
Rochester, MN 55905






From daemon Sat Apr 01 14:14:21 2000



From: Springett, Margaret J. :      hukee.margaret-at-mayo.edu
Date: Fri, 31 Mar 2000 10:11:18 -0600
Subject: RE: nickel grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




} ----------
} From: Springett, Margaret J.
} Sent: Friday, March 31, 2000 11:04 AM
} To: 'springett.margaret-at-mayo.edu'
} Subject: nickel grids
}
} I do an extensive amount of immunolabeling, and nickel grids are
} economical,
} but two factors need to be considered on a daily basis. The fact that
} they
} are magnetic can be an advantage as well as a detriment. Their magnetic
} quality allows one to rinse grids floating on a bubble with a magnetic
} stir
} plate, this has been demonstrated in Dr. Bendyan's workshops on
} goldlabeling
} techniques. When trying to retreive a stubborn grid out of a grid box, a
} magnetic tweezer is "heaven sent". Now about magnetic grids in the scope,
} if you stigmate the scope after fine-focusing, your micrograph will be
} focused. I have used these techniques to produce excellent micrographs of
} caveolae at better than 100K magnification. For our use gold grids are
} too
} expensive, so "work around methods" with nickel grids are necessary.
} Marge
}
}
Margaret Springett
IEM Specialist
Electron Microscopy Core Facility
Mayo Foundation and Clinic
Rochester, MN 55905






From daemon Sat Apr 01 14:14:22 2000



From: William McManus :      billemac-at-biology.usu.edu
Date: Fri, 31 Mar 2000 09:20:25 -0700
Subject: Operator Horror Stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We once had a student twist the condenser control knob off a TEM. When asked
to turn the knob clockwise, he just kept turning, until he handed me the
knob.

William McManus
Supervisor
Electron Microscopy Facility
Department of Biology
Utah State University
Logan UT 84322-5305

billEMac-at-cc.usu.edu
435-797-1920


-----Original Message-----
} From: Nestor J. Zaluzec [mailto:zaluzec-at-sparc5.microscopy.com]
Sent: Friday, March 31, 2000 7:02 AM
To: Microscopy-at-sparc5.microscopy.com



Originally posted by: sryazant-at-ucla.edu




It is not "horror" story, but a sort of... Many years ago the colleague
from friendly Lab visited me with great project. The idea was simply and
beautiful. She argues that because the image in the scope (TEM) is green
(green fluorescence of the screen), she wants to modify the sample (I
forgot what, some protein, I believe) by red-fluorescent dye to be able to
see on the screen of the electron microscope the "double-staining": red on
the green background. No comments...

Sergey

} Date: Thu, 30 Mar 2000 09:17:56 -0500
} From: "anderron-at-us.ibm.com"-at-sparc5.microscopy.com
} Subject: Operator Horror Stories
} To: microscopy-at-sparc5.microscopy.com
} Importance: Normal
} X-MIMETrack: Serialize by Router on D01ML065/01/M/IBM(Release 5.0.2b
(Intl)|18
} January 2000) at 30/03/2000 09:18:01
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu
http://www.bol.ucla.edu/~sryazant





From daemon Sat Apr 01 14:14:23 2000



From: William McManus :      billemac-at-biology.usu.edu
Date: Fri, 31 Mar 2000 09:20:25 -0700
Subject: Operator Horror Stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We once had a student twist the condenser control knob off a TEM. When asked
to turn the knob clockwise, he just kept turning, until he handed me the
knob.

William McManus
Supervisor
Electron Microscopy Facility
Department of Biology
Utah State University
Logan UT 84322-5305

billEMac-at-cc.usu.edu
435-797-1920


-----Original Message-----
} From: Nestor J. Zaluzec [mailto:zaluzec-at-sparc5.microscopy.com]
Sent: Friday, March 31, 2000 7:02 AM
To: Microscopy-at-sparc5.microscopy.com



Originally posted by: sryazant-at-ucla.edu




It is not "horror" story, but a sort of... Many years ago the colleague
from friendly Lab visited me with great project. The idea was simply and
beautiful. She argues that because the image in the scope (TEM) is green
(green fluorescence of the screen), she wants to modify the sample (I
forgot what, some protein, I believe) by red-fluorescent dye to be able to
see on the screen of the electron microscope the "double-staining": red on
the green background. No comments...

Sergey

} Date: Thu, 30 Mar 2000 09:17:56 -0500
} From: "anderron-at-us.ibm.com"-at-sparc5.microscopy.com
} Subject: Operator Horror Stories
} To: microscopy-at-sparc5.microscopy.com
} Importance: Normal
} X-MIMETrack: Serialize by Router on D01ML065/01/M/IBM(Release 5.0.2b
(Intl)|18
} January 2000) at 30/03/2000 09:18:01
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu
http://www.bol.ucla.edu/~sryazant





From daemon Sat Apr 01 14:14:23 2000



From: Larry Allard :      l2a-at-ornl.gov
Date: Fri, 31 Mar 2000 11:43:34 -0500
Subject: Re: Ni grids with holey carbon film

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We get our Ni/holey carbon films from SPI, at http://www.2spi.com/.
They will prepare excellent holey carbon films on just about any
available grid material (for a price of course...).

Larry





} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Dr. Lawrence F. Allard
Senior Research Staff Member
High Temperature Materials Laboratory
Oak Ridge National Laboratory
1 Bethel Valley Road
Bldg. 4515, MS 6064
PO Box 2008
Oak Ridge, TN 37831-6064

423-574-4981
423-574-4913 Fax
l2a-at-ornl.gov



From daemon Sat Apr 01 14:14:24 2000



From: Larry Allard :      l2a-at-ornl.gov
Date: Fri, 31 Mar 2000 11:43:34 -0500
Subject: Re: Ni grids with holey carbon film

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We get our Ni/holey carbon films from SPI, at http://www.2spi.com/.
They will prepare excellent holey carbon films on just about any
available grid material (for a price of course...).

Larry





} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Dr. Lawrence F. Allard
Senior Research Staff Member
High Temperature Materials Laboratory
Oak Ridge National Laboratory
1 Bethel Valley Road
Bldg. 4515, MS 6064
PO Box 2008
Oak Ridge, TN 37831-6064

423-574-4981
423-574-4913 Fax
l2a-at-ornl.gov



From daemon Sun Apr 02 16:44:01 2000



From: Ladd Research :      ladres-at-worldnet.att.net
Date: Fri, 31 Mar 2000 12:27:46 -0500
Subject: Re: Ni grids with holey carbon film

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Nestor J. Zaluzec wrote:
}
} ------------------------------------------------------------------------} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.}
} Originally posted by: willem.erasmus-at-sasol.com
}
} Dear fellow microscopists
}
} Does anybody know where I can purchase Ni TEM grids with a holey carbon film
} ? I can find plenty of copper grids, but the Ni grids seem to be hard to
} find.
}
} Thanks
} W. Erasmus
}
} Willem Erasmus
} Snr. Scientist, Basic Catalysis Research
} Sasol Technology
} Tel : +27 +16 9603954
} Fax : +27 +16 9602826
} E-mail : willem.erasmus-at-sasol.com

We at Ladd Reseach can supply you with these, as would most of the other
supply houses that make their own holey film. Please let us know what
size mesh you want and a quantity and we can quote you.

Debbie Sicard
Sales Manager

Disclaimer: Ladd Research is a vendor that sells EM supplies.
--

LADD RESEARCH
131 Dorset Lane
Williston, VT 05495 USA

TEL 1-800-451-3406 (US) or 1-802-878-6711 (anywhere)
FAX 1-802-878-8074
e-mail ladres-at-worldnet.att.net
web site http://www.ladd.cc



From daemon Sun Apr 02 16:44:01 2000



From: Ladd Research :      ladres-at-worldnet.att.net
Date: Fri, 31 Mar 2000 12:27:46 -0500
Subject: Re: Ni grids with holey carbon film

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Nestor J. Zaluzec wrote:
}
} ------------------------------------------------------------------------} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.}
} Originally posted by: willem.erasmus-at-sasol.com
}
} Dear fellow microscopists
}
} Does anybody know where I can purchase Ni TEM grids with a holey carbon film
} ? I can find plenty of copper grids, but the Ni grids seem to be hard to
} find.
}
} Thanks
} W. Erasmus
}
} Willem Erasmus
} Snr. Scientist, Basic Catalysis Research
} Sasol Technology
} Tel : +27 +16 9603954
} Fax : +27 +16 9602826
} E-mail : willem.erasmus-at-sasol.com

We at Ladd Reseach can supply you with these, as would most of the other
supply houses that make their own holey film. Please let us know what
size mesh you want and a quantity and we can quote you.

Debbie Sicard
Sales Manager

Disclaimer: Ladd Research is a vendor that sells EM supplies.
--

LADD RESEARCH
131 Dorset Lane
Williston, VT 05495 USA

TEL 1-800-451-3406 (US) or 1-802-878-6711 (anywhere)
FAX 1-802-878-8074
e-mail ladres-at-worldnet.att.net
web site http://www.ladd.cc



From daemon Sun Apr 02 16:44:02 2000



From: Kriho, Virginia :      Vkriho-at-psych.uic.edu
Date: Fri, 31 Mar 2000 12:34:21 -0600
Subject: TEM/peroxidase immunocytochemistry

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am currently using the peroxidase/DAB pre-embedding method for
localization of a novel extracellular matrix protein. I am using 40 micron
vibratome sections. While my results give some extracellular labelling,
most of the labelling is associated with dendritic microtubules. Has anyone
experienced non-specific labelling of microtubules?



From daemon Sun Apr 02 16:44:02 2000



From: Kriho, Virginia :      Vkriho-at-psych.uic.edu
Date: Fri, 31 Mar 2000 12:34:21 -0600
Subject: TEM/peroxidase immunocytochemistry

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am currently using the peroxidase/DAB pre-embedding method for
localization of a novel extracellular matrix protein. I am using 40 micron
vibratome sections. While my results give some extracellular labelling,
most of the labelling is associated with dendritic microtubules. Has anyone
experienced non-specific labelling of microtubules?



From daemon Sun Apr 02 16:44:03 2000



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Fri, 31 Mar 2000 13:39:47 -0500
Subject: Carbon coated Ni grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

W. Erasmus asked:
================================================
Does anybody know where I can purchase Ni TEM grids with a holey carbon film
? I can find plenty of copper grids, but the Ni grids seem to be hard to
find.
=================================================
Some times the coating of Ni grids is a bit more difficult than the coating
of copper grids. This translates to somewhat lower yields and sometimes
higher prices. However, SPI Supplies regularly produces custom coated Ni
grids on whatever mesh and grid the customer specifies and at only a slight
price premium over copper.

Contact me off line for details or visit our webpage URL
http://www.2spi.com/catalog/grids/cusctgrd.html

Some of the other major suppliers of consumables also will coat Ni grids, or
at least that is my understanding.

Chuck
============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.spi.cc
########################
============================================





From daemon Sun Apr 02 16:44:03 2000



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Fri, 31 Mar 2000 13:39:47 -0500
Subject: Carbon coated Ni grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

W. Erasmus asked:
================================================
Does anybody know where I can purchase Ni TEM grids with a holey carbon film
? I can find plenty of copper grids, but the Ni grids seem to be hard to
find.
=================================================
Some times the coating of Ni grids is a bit more difficult than the coating
of copper grids. This translates to somewhat lower yields and sometimes
higher prices. However, SPI Supplies regularly produces custom coated Ni
grids on whatever mesh and grid the customer specifies and at only a slight
price premium over copper.

Contact me off line for details or visit our webpage URL
http://www.2spi.com/catalog/grids/cusctgrd.html

Some of the other major suppliers of consumables also will coat Ni grids, or
at least that is my understanding.

Chuck
============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.spi.cc
########################
============================================





From daemon Sun Apr 02 16:44:03 2000



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Fri, 31 Mar 2000 08:45:16 -1000 (HST)
Subject: RE: Operator Horror Stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


One day a user of our TEM complained that she could see the beam with the
specimen out, but as soon as she put in a grid the screen went
black. After playing with it awhile and ruling out magnetism or something
on the grid holder, I decided that there must be some small magnetic
particle in the stage area that was being pushed around by the grid
holder. Of course the service technician I got by phone wanted me to
begin taking the column apart from the gun on down, but I grew impatient
with this and soon went straight for the stage. I figured I would be
looking for some small metal sliver. Imagine my surprise when I found an
entire plastic pipettortip lying across the bore through the
anticontamination plate in the column! It seems the previous user had
been using these tips as forceps covers, and mysteriously lost one the day
before. It had gone into the column through the airlock with her grid,
not impossible with the Zeiss 10 top-loading system. Although not the
only strange thing I have found in that microscope's column, at about an
inch and a half long it is the largest.

Aloha,
Tina
****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************





From daemon Sun Apr 02 16:44:04 2000



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Fri, 31 Mar 2000 08:45:16 -1000 (HST)
Subject: RE: Operator Horror Stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


One day a user of our TEM complained that she could see the beam with the
specimen out, but as soon as she put in a grid the screen went
black. After playing with it awhile and ruling out magnetism or something
on the grid holder, I decided that there must be some small magnetic
particle in the stage area that was being pushed around by the grid
holder. Of course the service technician I got by phone wanted me to
begin taking the column apart from the gun on down, but I grew impatient
with this and soon went straight for the stage. I figured I would be
looking for some small metal sliver. Imagine my surprise when I found an
entire plastic pipettortip lying across the bore through the
anticontamination plate in the column! It seems the previous user had
been using these tips as forceps covers, and mysteriously lost one the day
before. It had gone into the column through the airlock with her grid,
not impossible with the Zeiss 10 top-loading system. Although not the
only strange thing I have found in that microscope's column, at about an
inch and a half long it is the largest.

Aloha,
Tina
****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************





From daemon Sun Apr 02 16:44:04 2000



From: MICHAEL DELANNOY :      delannoy-at-mail.jhmi.edu
Date: Fri, 31 Mar 2000 14:48:58 -0500 (EST)
Subject: Core Facility solvency

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Microscpy Core Managers,
Are there any EM/Confocal Microscope facilities within academia that
are running in the black or at least breaking even without a subsidy. If
so what are your rates and overhead, are your salaries included? Also are
there any companies or facilities that do tele-microscopy? All info will be
shared with the listserver, unless confidentiality is requested. Thank You.

Michael Delannoy
Basic Science Microscopy Facility
JHMI




From daemon Sun Apr 02 16:44:05 2000



From: MICHAEL DELANNOY :      delannoy-at-mail.jhmi.edu
Date: Fri, 31 Mar 2000 14:48:58 -0500 (EST)
Subject: Core Facility solvency

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Microscpy Core Managers,
Are there any EM/Confocal Microscope facilities within academia that
are running in the black or at least breaking even without a subsidy. If
so what are your rates and overhead, are your salaries included? Also are
there any companies or facilities that do tele-microscopy? All info will be
shared with the listserver, unless confidentiality is requested. Thank You.

Michael Delannoy
Basic Science Microscopy Facility
JHMI




From daemon Sun Apr 02 16:44:05 2000



From: White, Woody N :      Woody.N.White-at-mcdermott.com
Date: Fri, 31 Mar 2000 15:46:00 -0600
Subject: SEM Horror Story

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Many, many years ago... I was asked to evaluate particle size of a powder.
The
powder was dried sludge from a radioactive waste storage tank. My downfall
was
permitting a co-worker to prepare the sample. When I received it, rather
than a
thin layer on carbon tape, it was caked on. Furthermore, the powder turned
out
to be sub-micron in size and almost pure Sr-90! Static and air currents
began
to spread contamination, even before I got it in the chamber. I quickly
left
the area before I became "hot" too.

I did finish the exam... In anti-contamination dress with a full face
respirator at the console!

The job took a few hours. It took two weeks for me and my service engineer
to
disassemble the SEM, clean (and the room) it to reasonable levels and
reassemble. The vacuum system was rather "warm" from that time on...
Nevermore! Nevermore!

Moral: Always insist on making input and final approval if someone else
wants
to prepare your samples.

Woody White
McDermott Technology

My new web URL:
http://woody.white.home.att.net



From daemon Sun Apr 02 16:44:06 2000



From: White, Woody N :      Woody.N.White-at-mcdermott.com
Date: Fri, 31 Mar 2000 15:46:00 -0600
Subject: SEM Horror Story

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Many, many years ago... I was asked to evaluate particle size of a powder.
The
powder was dried sludge from a radioactive waste storage tank. My downfall
was
permitting a co-worker to prepare the sample. When I received it, rather
than a
thin layer on carbon tape, it was caked on. Furthermore, the powder turned
out
to be sub-micron in size and almost pure Sr-90! Static and air currents
began
to spread contamination, even before I got it in the chamber. I quickly
left
the area before I became "hot" too.

I did finish the exam... In anti-contamination dress with a full face
respirator at the console!

The job took a few hours. It took two weeks for me and my service engineer
to
disassemble the SEM, clean (and the room) it to reasonable levels and
reassemble. The vacuum system was rather "warm" from that time on...
Nevermore! Nevermore!

Moral: Always insist on making input and final approval if someone else
wants
to prepare your samples.

Woody White
McDermott Technology

My new web URL:
http://woody.white.home.att.net



From daemon Sun Apr 02 16:44:06 2000



From: john grazul :      grazul-at-physics.bell-labs.com
Date: Fri, 31 Mar 2000 16:48:24 -0500
Subject: Re: Operator Horror Stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


This is my second go around with this, I got the Nestor porno bump this
morning.

I had a student using my JEOL 100 CX II; loading and looking around went
well. When she went to remove the holder she got half way out and yanked it
toward her. This action put a 70 degree bend in the holder. I could never
get the Z height to behave after that for some odd reason.

The other disaster was with my Philips 300, complete with Mercury Dif pump
at that time. I needed to do some anode cleaning, so without deflating the
air table I got on the console finished my work and got off. When the scope
lurched to the right it dumped the contents of the mercury pump into the oil
pump. This sent a cloud of mercury up the column creating an alloy wherever
it went.

Believe it or not the scope is in fine working order with not a trace of
mercury left in in the column. How did I do it? If you can figure it out I
will send the winner a Lucent/Bell Labs brief case...at my cost of course.

John Grazul
Lucent Technologies

"W. L. Steffens" wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} I had one faculty operator several years ago who after a SINGLE lesson
} on the Phillips 200, decided to come back late at night and look some
} more. After taking some images, he couldn't get the door to the camera
} chamber off, since he had failed to vent it. After rummaging through
} the lab, he found a large screwdriver and commenced to pry the camera
} door off, unconcerned about the marks and gouges that he was putting in
} the brass. Needless to say, when the vacuum was finally broken, the
} mercury diffusion pump became very unhappy. Eventually we were able to
} replace the instrument and the user finally left after being denied
} tenure.
}
} W. L. Steffens, Ph.D
} Dept. of Pathology
} University of Georgia




From daemon Sun Apr 02 16:44:07 2000



From: john grazul :      grazul-at-physics.bell-labs.com
Date: Fri, 31 Mar 2000 16:48:24 -0500
Subject: Re: Operator Horror Stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


This is my second go around with this, I got the Nestor porno bump this
morning.

I had a student using my JEOL 100 CX II; loading and looking around went
well. When she went to remove the holder she got half way out and yanked it
toward her. This action put a 70 degree bend in the holder. I could never
get the Z height to behave after that for some odd reason.

The other disaster was with my Philips 300, complete with Mercury Dif pump
at that time. I needed to do some anode cleaning, so without deflating the
air table I got on the console finished my work and got off. When the scope
lurched to the right it dumped the contents of the mercury pump into the oil
pump. This sent a cloud of mercury up the column creating an alloy wherever
it went.

Believe it or not the scope is in fine working order with not a trace of
mercury left in in the column. How did I do it? If you can figure it out I
will send the winner a Lucent/Bell Labs brief case...at my cost of course.

John Grazul
Lucent Technologies

"W. L. Steffens" wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} I had one faculty operator several years ago who after a SINGLE lesson
} on the Phillips 200, decided to come back late at night and look some
} more. After taking some images, he couldn't get the door to the camera
} chamber off, since he had failed to vent it. After rummaging through
} the lab, he found a large screwdriver and commenced to pry the camera
} door off, unconcerned about the marks and gouges that he was putting in
} the brass. Needless to say, when the vacuum was finally broken, the
} mercury diffusion pump became very unhappy. Eventually we were able to
} replace the instrument and the user finally left after being denied
} tenure.
}
} W. L. Steffens, Ph.D
} Dept. of Pathology
} University of Georgia




From daemon Sun Apr 02 16:44:08 2000



From: Lou Ann Miller :      lamiller-at-ux1.cso.uiuc.edu
Date: Fri, 31 Mar 2000 16:40:27 -0600
Subject: Horror Inspection

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Once, over a decade back, I was working EM and Med Tech at the same
time at a local hospital..

We had a CAP inspection, the officers were from another hospital in the state.


The question I was expected to reason and answer with a straight face was:


* What other protective measures besides standing behind a lead
wall do you take when you put the glass slide into the TEM, to
protect yourself from the flying electrons???



seriously!

I was rather stunned, and started looking from the wall socket to
him and back, but he didn't catch the connection.


I then tried to tactfully explain that vacuum was required, and that
we really didn't need that extra protection!

It was explained to me that he did too know all about EM because he
had a one day workshop.



Lou Ann
{ { { { { { { { { { { { { { { { { {} } } } } } } } } } } } } } } } } } } } } } } } }
Lou Ann Miller
Service Supervisor

Rm 1108 VMBSBld.
College of Veterinary Medicine
Veterinary Biosciences
2001 S. Lincoln Ave
Urbana, IL 61802

Phone: 217-244-1567
Fax: 217-244-1652

lamiller-at-uiuc.edu
lamiller-at-net66.com
turtle_lam-at-yahoo.com

Web Pages:
Lab Page
http://treefrog.cvm.uiuc.edu
Centeral States Microscopy Page
http://treefrog.cvm.uiuc.edu/csmms
Personal Home Page
http://treefrog.cvm.uiuc.edu/lam



From daemon Sun Apr 02 16:44:08 2000



From: Lou Ann Miller :      lamiller-at-ux1.cso.uiuc.edu
Date: Fri, 31 Mar 2000 16:40:27 -0600
Subject: Horror Inspection

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Once, over a decade back, I was working EM and Med Tech at the same
time at a local hospital..

We had a CAP inspection, the officers were from another hospital in the state.


The question I was expected to reason and answer with a straight face was:


* What other protective measures besides standing behind a lead
wall do you take when you put the glass slide into the TEM, to
protect yourself from the flying electrons???



seriously!

I was rather stunned, and started looking from the wall socket to
him and back, but he didn't catch the connection.


I then tried to tactfully explain that vacuum was required, and that
we really didn't need that extra protection!

It was explained to me that he did too know all about EM because he
had a one day workshop.



Lou Ann
{ { { { { { { { { { { { { { { { { {} } } } } } } } } } } } } } } } } } } } } } } } }
Lou Ann Miller
Service Supervisor

Rm 1108 VMBSBld.
College of Veterinary Medicine
Veterinary Biosciences
2001 S. Lincoln Ave
Urbana, IL 61802

Phone: 217-244-1567
Fax: 217-244-1652

lamiller-at-uiuc.edu
lamiller-at-net66.com
turtle_lam-at-yahoo.com

Web Pages:
Lab Page
http://treefrog.cvm.uiuc.edu
Centeral States Microscopy Page
http://treefrog.cvm.uiuc.edu/csmms
Personal Home Page
http://treefrog.cvm.uiuc.edu/lam



From daemon Sun Apr 02 16:44:08 2000



From: David R Hull :      David.R.Hull-at-grc.nasa.gov
Date: Fri, 31 Mar 2000 17:58:37 -0500
Subject: Re: Operator Horror Stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} We once had an operator on our JEOL 840A SEM complain about her
} sample moving. Her sample was samples of vacuum grease she had gold
} coated and was attempting to image in the SEM. When the electron
} beam heated the grease it cracked up the gold coating and charged.
} She was using the SEM to look for silicon in various greases. I
} suggested she use a different technique so we did not end up with
} vaporized grease inside the SEM.

David R. Hull
NASA Glenn Research Center at Lewis Field
Advanced Metallics Branch
Mail Stop 49-1
21000 Brookpark Road
Cleveland, OH 44135

(216) 433-3281
fax (216)977- 7132
david.r.hull-at-grc.nasa.gov



From daemon Sun Apr 02 16:44:09 2000



From: David R Hull :      David.R.Hull-at-grc.nasa.gov
Date: Fri, 31 Mar 2000 17:58:37 -0500
Subject: Re: Operator Horror Stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} We once had an operator on our JEOL 840A SEM complain about her
} sample moving. Her sample was samples of vacuum grease she had gold
} coated and was attempting to image in the SEM. When the electron
} beam heated the grease it cracked up the gold coating and charged.
} She was using the SEM to look for silicon in various greases. I
} suggested she use a different technique so we did not end up with
} vaporized grease inside the SEM.

David R. Hull
NASA Glenn Research Center at Lewis Field
Advanced Metallics Branch
Mail Stop 49-1
21000 Brookpark Road
Cleveland, OH 44135

(216) 433-3281
fax (216)977- 7132
david.r.hull-at-grc.nasa.gov



From daemon Sun Apr 02 16:44:09 2000



From: Greg Strout :      gstrout-at-ou.edu
Date: Fri, 31 Mar 2000 16:48:19 -0600
Subject: Re: Operator Horror Stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


It seems everyone has one of these. I had a person who one day
complained of a burning smell while she was at the scope. I went to
investigate and when I arrived in the room there was no smell... burning
or otherwise. Several more times that session she came and got me to
investigate this burning smell in the TEM room. This continued for a
couple of sessions. I did go and investigate because after all, who
wants their TEM on fire? I honestly didn't know what to do about it and
was beginning to seriously question her sanity. Finally she convinced
me to sit with her while she operated the scope and sure enough there
was this faint smell of something burning! I jumped up and tried to
find it, but it had gone away. So...she sat down again and began to
work and the smell, like plastic kitchenware burning in a dishwasher,
came back. Again I jump up and look around....to no avail! I was
curious now. The burning smell only happens when she is sitting at the
scope. Not being a big believer in spontaneous combustion, there had to
be an answer! There was. As part of the TEM course I take the kick
panel off the Zeiss 10 to show the students the guts of the vacuum
system. The kick panel is right below the desk area where you sit up to
the microscope. What she was doing was placing her tennis shoe on the
heater of the diffusion pump and the plastic/rubber would melt and burn
a bit -- just enough to give the room a burning smell. She never did
notice her foot getting warm. When I pointed it out she did admit that
her shoe was warm and had some ugly scars on it to boot! She never
returned after that semester - I guess that she had enough of EM! Now I
carefully point out the hazard and replace the cover quickly when we are
finished with the class.

--
==================================================================
Greg Strout
Electron Microscopist, University of Oklahoma
WWW Virtual Library for Microscopy:
http://www.ou.edu/research/electron/www-vl/
e-mail: gstrout-at-ou.edu
Opinions expressed herein are mine and not necessarily those of
the University of Oklahoma
==================================================================





From daemon Sun Apr 02 16:44:09 2000



From: Greg Strout :      gstrout-at-ou.edu
Date: Fri, 31 Mar 2000 16:48:19 -0600
Subject: Re: Operator Horror Stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


It seems everyone has one of these. I had a person who one day
complained of a burning smell while she was at the scope. I went to
investigate and when I arrived in the room there was no smell... burning
or otherwise. Several more times that session she came and got me to
investigate this burning smell in the TEM room. This continued for a
couple of sessions. I did go and investigate because after all, who
wants their TEM on fire? I honestly didn't know what to do about it and
was beginning to seriously question her sanity. Finally she convinced
me to sit with her while she operated the scope and sure enough there
was this faint smell of something burning! I jumped up and tried to
find it, but it had gone away. So...she sat down again and began to
work and the smell, like plastic kitchenware burning in a dishwasher,
came back. Again I jump up and look around....to no avail! I was
curious now. The burning smell only happens when she is sitting at the
scope. Not being a big believer in spontaneous combustion, there had to
be an answer! There was. As part of the TEM course I take the kick
panel off the Zeiss 10 to show the students the guts of the vacuum
system. The kick panel is right below the desk area where you sit up to
the microscope. What she was doing was placing her tennis shoe on the
heater of the diffusion pump and the plastic/rubber would melt and burn
a bit -- just enough to give the room a burning smell. She never did
notice her foot getting warm. When I pointed it out she did admit that
her shoe was warm and had some ugly scars on it to boot! She never
returned after that semester - I guess that she had enough of EM! Now I
carefully point out the hazard and replace the cover quickly when we are
finished with the class.

--
==================================================================
Greg Strout
Electron Microscopist, University of Oklahoma
WWW Virtual Library for Microscopy:
http://www.ou.edu/research/electron/www-vl/
e-mail: gstrout-at-ou.edu
Opinions expressed herein are mine and not necessarily those of
the University of Oklahoma
==================================================================





From daemon Sun Apr 02 16:44:10 2000



From: Amy McGough :      amcgough-at-bcm.tmc.edu
Date: Fri, 31 Mar 2000 17:50:10 -0600
Subject: Position Announcement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Position Announcement

Research Scientist in Macromolecular Microscopy

Purdue University Department of Biological Sciences

An opening is available immediately for a scientist specializing in
macromolecular microscopy at Purdue University. The successful
candidate would be responsible for overseeing the day-to-day
operations of the high-resolution electron microscopy facility, training
new users, and assisting outside visitors with data collection and
analysis. He/she would also be expected to work with the cryoEM
groups in the department at planning and implementing the future
directions of the facility, including the development of new
technologies for improved specimen preservation, data collection, and
analysis. The ideal candidate should complement the existing
strengths represented in the department and pursue independent
research funding.

The Department of Biological Sciences at Purdue provides a rich
intellectual environment as well as outstanding modern scientific
facilities including Philips EM420, CM200-FEG, and CM300-FEG
electron cryo-microscopes. Purdue University is home to over 50,000
students, faculty, and staff, and is located in West Lafayette, Indiana -
approximately 1 hour northwest of Indianapolis and 2.5 hours
southeast of Chicago. The area affords the advantages associated with
a major university, while providing an affordable and attractive `small
town' environment in which to live.

Requirements for this position include a Ph.D. in biophysics or one of
the related sciences, a minimum of 5 years post-doctoral work
experience in electron cryo-microscopy of biological molecules, and a
proven ability to work well with others. Preference will be given to
candidates who have successfully developed new technologies and
methodologies for high-resolution electron cryo-microscopy. Salary
will be commensurate with experience and includes full benefits.

Interested individuals should send their resume, relevant reprints, a
statement of research interests and career goals, and provide contact
information (name/phone/e-mail) for three individuals who have
agreed to serve as references to:

Dr. Timothy S. Baker (tsb-at-bragg.bio.purdue.edu)

and/or

Dr. Amy McGough (amcgough-at-bcm.tmc.edu)

Department of Biological Sciences
Lilly Hall of Life Science
Purdue University
West Lafayette, IN 47907-1392

Purdue is an equal access/equal opportunity university.



From daemon Sun Apr 02 16:44:10 2000



From: Amy McGough :      amcgough-at-bcm.tmc.edu
Date: Fri, 31 Mar 2000 17:50:10 -0600
Subject: Position Announcement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Position Announcement

Research Scientist in Macromolecular Microscopy

Purdue University Department of Biological Sciences

An opening is available immediately for a scientist specializing in
macromolecular microscopy at Purdue University. The successful
candidate would be responsible for overseeing the day-to-day
operations of the high-resolution electron microscopy facility, training
new users, and assisting outside visitors with data collection and
analysis. He/she would also be expected to work with the cryoEM
groups in the department at planning and implementing the future
directions of the facility, including the development of new
technologies for improved specimen preservation, data collection, and
analysis. The ideal candidate should complement the existing
strengths represented in the department and pursue independent
research funding.

The Department of Biological Sciences at Purdue provides a rich
intellectual environment as well as outstanding modern scientific
facilities including Philips EM420, CM200-FEG, and CM300-FEG
electron cryo-microscopes. Purdue University is home to over 50,000
students, faculty, and staff, and is located in West Lafayette, Indiana -
approximately 1 hour northwest of Indianapolis and 2.5 hours
southeast of Chicago. The area affords the advantages associated with
a major university, while providing an affordable and attractive `small
town' environment in which to live.

Requirements for this position include a Ph.D. in biophysics or one of
the related sciences, a minimum of 5 years post-doctoral work
experience in electron cryo-microscopy of biological molecules, and a
proven ability to work well with others. Preference will be given to
candidates who have successfully developed new technologies and
methodologies for high-resolution electron cryo-microscopy. Salary
will be commensurate with experience and includes full benefits.

Interested individuals should send their resume, relevant reprints, a
statement of research interests and career goals, and provide contact
information (name/phone/e-mail) for three individuals who have
agreed to serve as references to:

Dr. Timothy S. Baker (tsb-at-bragg.bio.purdue.edu)

and/or

Dr. Amy McGough (amcgough-at-bcm.tmc.edu)

Department of Biological Sciences
Lilly Hall of Life Science
Purdue University
West Lafayette, IN 47907-1392

Purdue is an equal access/equal opportunity university.



From daemon Sun Apr 02 16:44:10 2000



From: John Hunt :      hunt-at-ccmr.cornell.edu
Date: Fri, 31 Mar 2000 18:10:58 -0600
Subject: Re: SEM - Coating

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Bart,
I believe Argon is used because it is a larger molecule and
sputters the metal more efficiently. I also use air with no problem.
Possibly the amount of metal sputtered is slightly less per unit time.
John Hunt

On Fri, 31 Mar 2000, Nestor J. Zaluzec wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} Originally posted by: bart.depauw-at-rug.ac.be
}
}
} Hello,
}
} I would like to know why they use Argon in the metal coating devices. At
} our laboratory, we don't use Argon, we use air. What is the advantage of
} using Argon ?
} De Pauw Bart
} Faculty of Veterinary Medicine
} Morphology
} Salisburylaan 133
} 9820 Merelbeke
} Belgium
} Phone : 0032(0)9 264.77.19
} Fax : 0032(0)9 264.77.90
}
}
}





From daemon Sun Apr 02 16:44:11 2000



From: Debby Sherman :      sherman-at-btny.purdue.edu
Date: Fri, 31 Mar 2000 18:12:12 -0600
Subject: Re: Operator Horror Stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Many years ago we had a Siemens EM-101. This was a relatively new
microscope and beautifully machined with German precision. The camera
chamber was a work of art. Each film plate (we used glass back then) was
encased in it's own light-tight cassette. The camera would move a film
cassette into position for exposure and pull off the cassette cover. The
operator would then expose the film and move the casette into a drop box.
The cassette cover would be pushed closed in the process.
This camera worked almost flawlessly. On occassion, an operator, when
loading the camera, would put the cassettes in on an angle. When you went
to take the first image, the cassette would jam and not move into position.
All that was necessary to unjam was to break vacuum, giggle the cassette
with your hand to straighten it and then repump the camera chamber and get
back to work...a 10 minute process as most.
We had one operator who thought she knew it all. She was working on the
microscope one evening and the camera jammed. Her solution was to take out
the camera and take it apart...screw by screw and spring by spring. She
ended up with an incredible heap of parts and of course had no clue as to
how to put it back together. Neither did the Siemens service engineers.
They had never seen one apart! They had to find another camera (not easy
in those days with relatively few of these instruments around), take it
apart..carefully marking where each piece came from...and then reassembled
each camera simultaneously. It took hours!!
Moral of the story...NO ONE fixed ANYTHING without permission of the lab
personnel!!



Debby Sherman, Manager Phone: 765-494-6666
Microscopy Center in Agriculture FAX: 765-494-5896
Dept. of Botany & Plant Pathology E-mail: sherman-at-btny.purdue.edu
Purdue University
1057 Whistler Building
West Lafayette, IN 47907-1057





From daemon Sun Apr 02 16:44:11 2000



From: John Hunt :      hunt-at-ccmr.cornell.edu
Date: Fri, 31 Mar 2000 18:10:58 -0600
Subject: Re: SEM - Coating

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Bart,
I believe Argon is used because it is a larger molecule and
sputters the metal more efficiently. I also use air with no problem.
Possibly the amount of metal sputtered is slightly less per unit time.
John Hunt

On Fri, 31 Mar 2000, Nestor J. Zaluzec wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} Originally posted by: bart.depauw-at-rug.ac.be
}
}
} Hello,
}
} I would like to know why they use Argon in the metal coating devices. At
} our laboratory, we don't use Argon, we use air. What is the advantage of
} using Argon ?
} De Pauw Bart
} Faculty of Veterinary Medicine
} Morphology
} Salisburylaan 133
} 9820 Merelbeke
} Belgium
} Phone : 0032(0)9 264.77.19
} Fax : 0032(0)9 264.77.90
}
}
}





From daemon Sun Apr 02 16:44:11 2000



From: Debby Sherman :      sherman-at-btny.purdue.edu
Date: Fri, 31 Mar 2000 18:12:12 -0600
Subject: Re: Operator Horror Stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Many years ago we had a Siemens EM-101. This was a relatively new
microscope and beautifully machined with German precision. The camera
chamber was a work of art. Each film plate (we used glass back then) was
encased in it's own light-tight cassette. The camera would move a film
cassette into position for exposure and pull off the cassette cover. The
operator would then expose the film and move the casette into a drop box.
The cassette cover would be pushed closed in the process.
This camera worked almost flawlessly. On occassion, an operator, when
loading the camera, would put the cassettes in on an angle. When you went
to take the first image, the cassette would jam and not move into position.
All that was necessary to unjam was to break vacuum, giggle the cassette
with your hand to straighten it and then repump the camera chamber and get
back to work...a 10 minute process as most.
We had one operator who thought she knew it all. She was working on the
microscope one evening and the camera jammed. Her solution was to take out
the camera and take it apart...screw by screw and spring by spring. She
ended up with an incredible heap of parts and of course had no clue as to
how to put it back together. Neither did the Siemens service engineers.
They had never seen one apart! They had to find another camera (not easy
in those days with relatively few of these instruments around), take it
apart..carefully marking where each piece came from...and then reassembled
each camera simultaneously. It took hours!!
Moral of the story...NO ONE fixed ANYTHING without permission of the lab
personnel!!



Debby Sherman, Manager Phone: 765-494-6666
Microscopy Center in Agriculture FAX: 765-494-5896
Dept. of Botany & Plant Pathology E-mail: sherman-at-btny.purdue.edu
Purdue University
1057 Whistler Building
West Lafayette, IN 47907-1057





From daemon Sun Apr 02 16:44:12 2000



From: pe13-at-cam.ac.uk -at-sparc5.microscopy.com
Date: Fri, 31 Mar 2000 18:11:27 -0600
Subject: Re: SEM - Coating

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Air contains water vapour, oxygen and carbon dioxide all of which can be
broken down in the plasma generated in a sputter coater and gve rise to
nasty active species which can damage delicate samples. Argon is used
because of its relative high atomic number and this ensures there is
multiple scattering of the sputtered metal. There is a Zenon sputter coater
on the market which should sputter even better but I have no experience
with it. Come to the Lehigh Short Course(contact {slc6-at-lehigh.edu) and we
will tell you all about it.

Patrick Echlin
Cambridge





From daemon Sun Apr 02 16:44:12 2000



From: pe13-at-cam.ac.uk -at-sparc5.microscopy.com
Date: Fri, 31 Mar 2000 18:11:27 -0600
Subject: Re: SEM - Coating

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Air contains water vapour, oxygen and carbon dioxide all of which can be
broken down in the plasma generated in a sputter coater and gve rise to
nasty active species which can damage delicate samples. Argon is used
because of its relative high atomic number and this ensures there is
multiple scattering of the sputtered metal. There is a Zenon sputter coater
on the market which should sputter even better but I have no experience
with it. Come to the Lehigh Short Course(contact {slc6-at-lehigh.edu) and we
will tell you all about it.

Patrick Echlin
Cambridge





From daemon Sun Apr 02 16:44:16 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 31 Mar 2000 19:37:29 -0600
Subject: Sputter coater availability before a new one

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am preparing to purchase a sputter coater for Au/Pd SEM
specimen preparation. I have narrowed the search to the VG-Scientific
Polaron SC7620. My old Polaron E5100 is very tired.

I have located used Hummer VI, Fullam EMS-76M and Denton
Desk II coaters for about $1000 less than new. Some of these
include the pump. However, I have several dual stage mech
pumps, so this is not an issue.

If anyone knows of a source for a good/recent used coater,
I would appreciate knowing about it. Otherwise, I will go with
a new one.

tnx,
gary g.




From daemon Sun Apr 02 16:44:17 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 31 Mar 2000 19:37:29 -0600
Subject: Sputter coater availability before a new one

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am preparing to purchase a sputter coater for Au/Pd SEM
specimen preparation. I have narrowed the search to the VG-Scientific
Polaron SC7620. My old Polaron E5100 is very tired.

I have located used Hummer VI, Fullam EMS-76M and Denton
Desk II coaters for about $1000 less than new. Some of these
include the pump. However, I have several dual stage mech
pumps, so this is not an issue.

If anyone knows of a source for a good/recent used coater,
I would appreciate knowing about it. Otherwise, I will go with
a new one.

tnx,
gary g.




From daemon Sun Apr 02 16:44:24 2000



From: jim :      jim-at-proscitech.com.au
Date: Mon, 3 Apr 2000 00:00:21 +1000
Subject: RE: LM/EM Embedding Oven

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I appreciate the comments by David Cheshire. Perhaps he could elaborate why a
place (not necessarily a Dive Shop) certified for the testing of diving
cylinders and regulators could not cope with a CPD? Is the implication that
these testing places can not be trusted (sorry divers), or that a CPD is more
complex than other equipment, or, horror, could these places be cheaper to get
a CPD tested. and certified. Surely, hydrostatic testing is rather similar and
certification and the relevant legislation would be for pressure vessels and
not just for CPD.

I still believe that testing of CPD is counter-productive, but if required by
legislation, then there is no option available.

I believe that CPD windows used to be quartz. This may well have changed and
some manufacturers may now use other material e.g."bullet proof glass", which
of course is a plastic. David Cheshire would have been more helpful to devulge
which material is used. This would be of great interest because some people
have used organic solvents as an intermediate fluid. In that case it would be
important to know what solvents affect this "far denser and tougher material".
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

-----Original Message-----
Sent: Saturday, April 01, 2000 12:03 AM
To: MICROSCOPY BB


A couple of suggestions that may help your collogue and others in regards to
ovens. Please note that PST sells ovens, but not to North America (wrong volts,
costly shipping).

For resin curing most people purchase the cheapest oven type, which relies on
convection currents to achieve reasonably even temperatures. Fan forced and
jacketed ovens have greater temperature uniformity within the chamber. (See
diagrams of oven types in our online: Home} Contents} E3} "click here" link) Its
wise to place specimens always in a similar position within the chamber.

A thermostat control the heating elements and commonly they switch within 1.5
degree C. Metals absorb heat faster and conduct heat faster into specimens. It
is quite possible to part-melt polyethylene capsules where they are in contact
with metal. The simple solution is to place the specimens on a piece of wood or
thick cardboard.

The offending oven may have a thermostat that switches to its own strange
rhythm. More likely though, it has an unacceptably wide range when turning the
element on and off. A possible solution to this problem is to enclose the
specimen in an insulating box within the oven. This should cause the highs and
lows to be leveled. That insulating box could be a small lid-less cardbox,
upended over the specimen and its insulating support.

Uneven polymerisation is often due to varying times within the oven. An easy
solution is a "lamplighter" timer. Plug the oven into this 24 hour timer and
adjust the timer to come on at say 5 pm and switch off at 8 am. Specimen can be
placed in the oven at any time and removed any time during the next day and
cure for the same number of hours. Fewer fumes within the lab is another
benefit.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Friday, March 31, 2000 4:02 AM, Paula Allan-Wojtas
[SMTP:ALLANWOJTASP-at-EM.AGR.CA] wrote:
}
}
} Hi, All,
}
} I am posting a message for a colleague who does not subscribe to this list.
}
} He is looking for small embedding oven which can polymerize resins in the
} range of 40?-70?C, but which has a very precise temperature control (holds
} the temperature well, with very little fluctuation during polymerization). He
} is having problems with the oven he presently has because the temperature is
} not controlled well enough, and he is getting uneven polymerization.
}
} Another condition is that he is in the middle of a project and needs the oven
} right away. He had an oven in mind, but it could not be delivered for 6
} weeks!!!
}
} Any suggestions from users or vendors are welcome. Please contact me offline,
} and I'll forward the replies to my colleague.
}
} Thanks again for all your help.
}
} Paula.
}
} Paula Allan-Wojtas
} Research Scientist, Food Microstructure
} Agriculture and Agri-Food Canada
} Atlantic Food and Horticulture Research Centre
} Kentville, Nova Scotia Canada B4N 1J5
}
} Tel: (902) 679-5566
} FAX: (902) 679-2311
}
} email: allanwojtasp-at-em.agr.ca
}



From daemon Sun Apr 02 16:44:30 2000



From: Damian :      dneuberger-at-mindspring.com
Date: Fri, 31 Mar 2000 22:57:16 -0600
Subject: RE: SEM Horror Story

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


These are great stories and would make a wonderful collection, especially
to hand out to students of EM. Yes, experience is a great way to learn but
it's often best, and cheaper, to learn from some else's.

Damian Neuberger




From daemon Sun Apr 02 16:44:30 2000



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Sat, 01 Apr 2000 10:13:34 -0500
Subject: Microscope nightmares

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Here is a "just off the press" example (but this does come up at least once
a year):

I fielded a phone call from a distraught SEM lab manager who told me that
some months ago he put Dow Corning fluid into his diffusion pump "to save
money". And now that he has had an accident, the silicone fluid of course,
has contaminated his system, so he was asking us, "what organic solvent will
easily remove it."

He was especially upset when I told him that his EDS detector will see Si
everywhere also, because they do a lot of analyses for Si!

I won't repeat what he told me when I tried to explain the reality of his
situation......

But it does go to show that there are a lot of new people entering our
profession, some with less training and experience with vacuum than others,
so such stories are very well worth repeating.

But just out of curiosity, is there some "recommended procedure" for
removing silicone fluid from the internal parts of a column, and also
removing it from the window of an EDS detector? I presume one can always
call in an outside service provider with experienced people but a lot of
users out there just don't have the budget for something like that. But
they do have a good supply of student manpower.

While we are on the subject of silicone, a few weeks ago a well known TEM
user got me on the phone to say "hello" and commented that he had just
placed an order for silicone grease and yes, he said, he was going to be
using it on the o rings of his column. I told him I thought that he should
be using other greases and his response was "don't listen to dogma, I
thought you read the listserver!". Am I correct, namely that one should not
be using silicone grease on the o rings of a column instrument?

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.spi.cc
########################
============================================





From daemon Sun Apr 02 16:44:30 2000



From: Lou Solebello :      microls1297-at-mindspring.com
Date: Friday, March 31, 2000 9:26 PM
Subject: Storys

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


My story is not as much of a horror as it is embarassing. Several years
back I received a package of samples from a regular client without any paper
work describing what the samples were. This is not unusual since we
frequently recieve unknowns. I proceeded to open the package and was
abruptly assaulted with an extremely strong odor of bannanas. Apparantly one
of the samples was a vial of concentrated bannana flavoring. It was months
(almost a year) before the odor completely disappeared from my office, and I
was cajoled about it frequently. I by the way, hate bannanas.............

Lou Solebello
-----Original Message-----
} From: L R MELSEN {lmelsen-at-emory.edu}
To: Microscopy-at-sparc5.microscopy.com {Microscopy-at-sparc5.microscopy.com}




From daemon Sun Apr 02 16:44:31 2000



From: Damian :      dneuberger-at-mindspring.com
Date: Fri, 31 Mar 2000 22:57:16 -0600
Subject: RE: SEM Horror Story

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


These are great stories and would make a wonderful collection, especially
to hand out to students of EM. Yes, experience is a great way to learn but
it's often best, and cheaper, to learn from some else's.

Damian Neuberger




From daemon Sun Apr 02 16:44:31 2000



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Sat, 01 Apr 2000 10:13:34 -0500
Subject: Microscope nightmares

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Here is a "just off the press" example (but this does come up at least once
a year):

I fielded a phone call from a distraught SEM lab manager who told me that
some months ago he put Dow Corning fluid into his diffusion pump "to save
money". And now that he has had an accident, the silicone fluid of course,
has contaminated his system, so he was asking us, "what organic solvent will
easily remove it."

He was especially upset when I told him that his EDS detector will see Si
everywhere also, because they do a lot of analyses for Si!

I won't repeat what he told me when I tried to explain the reality of his
situation......

But it does go to show that there are a lot of new people entering our
profession, some with less training and experience with vacuum than others,
so such stories are very well worth repeating.

But just out of curiosity, is there some "recommended procedure" for
removing silicone fluid from the internal parts of a column, and also
removing it from the window of an EDS detector? I presume one can always
call in an outside service provider with experienced people but a lot of
users out there just don't have the budget for something like that. But
they do have a good supply of student manpower.

While we are on the subject of silicone, a few weeks ago a well known TEM
user got me on the phone to say "hello" and commented that he had just
placed an order for silicone grease and yes, he said, he was going to be
using it on the o rings of his column. I told him I thought that he should
be using other greases and his response was "don't listen to dogma, I
thought you read the listserver!". Am I correct, namely that one should not
be using silicone grease on the o rings of a column instrument?

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.spi.cc
########################
============================================





From daemon Sun Apr 02 16:44:32 2000



From: Lou Solebello :      microls1297-at-mindspring.com
Date: Friday, March 31, 2000 9:26 PM
Subject: Storys

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


My story is not as much of a horror as it is embarassing. Several years
back I received a package of samples from a regular client without any paper
work describing what the samples were. This is not unusual since we
frequently recieve unknowns. I proceeded to open the package and was
abruptly assaulted with an extremely strong odor of bannanas. Apparantly one
of the samples was a vial of concentrated bannana flavoring. It was months
(almost a year) before the odor completely disappeared from my office, and I
was cajoled about it frequently. I by the way, hate bannanas.............

Lou Solebello
-----Original Message-----
} From: L R MELSEN {lmelsen-at-emory.edu}
To: Microscopy-at-sparc5.microscopy.com {Microscopy-at-sparc5.microscopy.com}




From daemon Sun Apr 02 16:44:32 2000



From: Frederick Schamber :      fhscham-at-sgi.net
Date: Sat, 01 Apr 2000 12:22:10 -0500
Subject: Horror Stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am enjoying the "horror stories" (don't we all enjoy hearing about
someone else's stupid mistake) and thought I would share one from the
"software" category.

I had just joined Northern Scientific (later Tracor Northern, now Noran)
and was writing EDS software for our first computerized analyzer (the
venerable NS-880) -- the year was 1972, and my software, like any of
the stuff being produced in those years where we wrote software on paper
tape for machines with 4K memories, just wasn't all that "user
friendly", so it was not unusual to have to walk people through
difficult command sequences. Then too, computers were pretty new to
everyone and people sometimes made silly-sounding mistakes out of pure
inexperience (one highly intelligent customer, when instructed to 'type
a space', dutifully typed "A SPACE" on the keyboard). One particular
customer, however, defied all attempts at instruction and had passed
well beyond "complaining" into the realm of "abusive" and "insulting".
He insisted that he was following the instructions to the letter, but
that our miserable software consistently malfunctioned. We were unable
to duplicate the problem and finally several of us drove a considerable
distance to his lab to get to the bottom of this. We went through the
operations with him watching intently and the software performed
flawlessly, which he acknowledged. We then asked him to operate the
software himself and midway through, observed that he was responding to
one of the dialog questions with a decidedly inappropriate response. We
had him back out of this and repeat it with the correct response and the
software then ran perfectly. To which his response was to draw himself
up and state with great authority and indignation: "but when you run it
THE WAY I DO, it doesn't work!"

Fred Schamber
RJ Lee Instruments Limited




From daemon Sun Apr 02 16:44:33 2000



From: Frederick Schamber :      fhscham-at-sgi.net
Date: Sat, 01 Apr 2000 12:22:10 -0500
Subject: Horror Stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am enjoying the "horror stories" (don't we all enjoy hearing about
someone else's stupid mistake) and thought I would share one from the
"software" category.

I had just joined Northern Scientific (later Tracor Northern, now Noran)
and was writing EDS software for our first computerized analyzer (the
venerable NS-880) -- the year was 1972, and my software, like any of
the stuff being produced in those years where we wrote software on paper
tape for machines with 4K memories, just wasn't all that "user
friendly", so it was not unusual to have to walk people through
difficult command sequences. Then too, computers were pretty new to
everyone and people sometimes made silly-sounding mistakes out of pure
inexperience (one highly intelligent customer, when instructed to 'type
a space', dutifully typed "A SPACE" on the keyboard). One particular
customer, however, defied all attempts at instruction and had passed
well beyond "complaining" into the realm of "abusive" and "insulting".
He insisted that he was following the instructions to the letter, but
that our miserable software consistently malfunctioned. We were unable
to duplicate the problem and finally several of us drove a considerable
distance to his lab to get to the bottom of this. We went through the
operations with him watching intently and the software performed
flawlessly, which he acknowledged. We then asked him to operate the
software himself and midway through, observed that he was responding to
one of the dialog questions with a decidedly inappropriate response. We
had him back out of this and repeat it with the correct response and the
software then ran perfectly. To which his response was to draw himself
up and state with great authority and indignation: "but when you run it
THE WAY I DO, it doesn't work!"

Fred Schamber
RJ Lee Instruments Limited




From daemon Sun Apr 02 16:44:33 2000



From: Keith Ryan :      KPR-at-wpo.nerc.ac.uk
Date: Sun, 02 Apr 2000 21:16:37 +0100
Subject: Re: Operator Horror Stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have a similar story to this one! A bright fellow from a senior
University (I would embarrass them) had a dim TEM image, so rigged up
an Anglepoise lamp to try and throw some more light on it!

Keith Ryan
Marine Biological Association
Plymouth, UK

} } } Larry Allard {l2a-at-ornl.gov} 03/31/00 06:08pm } } }
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} Humorous, but not quite horror:


The respected PhD I once worked with on our old JEOL JSM-U3 scanner,

who was having trouble making out the details of the image on the
monitor, so grabbed a flashlight and shined it at the screen so he
could see the image a little better... :-).

Larry

}
}
} Originally posted by: sryazant-at-ucla.edu
}
}
}
}
} It is not "horror" story, but a sort of... Many years ago the
colleague
} from friendly Lab visited me with great project. The idea was
simply and
} beautiful. She argues that because the image in the scope (TEM) is
green
} (green fluorescence of the screen), she wants to modify the sample
(I
} forgot what, some protein, I believe) by red-fluorescent dye to be
able to
} see on the screen of the electron microscope the "double-staining":
red on
} the green background. No comments...
}
} Sergey
}
} } Date: Thu, 30 Mar 2000 09:17:56 -0500
} } From: "anderron-at-us.ibm.com"-at-sparc5.microscopy.com
} } Subject: Operator Horror Stories
} } To: microscopy-at-sparc5.microscopy.com
} } Importance: Normal
} } X-MIMETrack: Serialize by Router on D01ML065/01/M/IBM(Release
5.0.2b
} (Intl)|18
} } January 2000) at 30/03/2000 09:18:01
} }
}
} ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
} } To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com

} } On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html

}
} -----------------------------------------------------------------------.
} }
} }
} }
} } Alan Fox, in a response to the Analytical TEM string, cautioned
the
} } question-raiser to beware of people who abuse instruments.
Perhaps Nestor
} } will forgive a little microscopy related humor and allow us to
start a
} } string on "Operator Horror Stories." Here's ours:
} }
} } The "ham-fisted user" reference made us chuckle/cringe with the
memory of
} } a guest "microscopist" in our lab who hauled himself (220 pounds
or so) out
} } of his seat by pulling on the half-inserted specimen rod, bending
it about
} } 20 degrees or so!
} }
} } Henceforth, when we saw him in the hall (he never came into the
scope room
} } again), we referred to him as "Conan the Microscopist"!
} }
} }
} }
} } Ron Anderson, IBM, Hopewell Jct., New York, USA.
anderron-at-us.ibm.com

} }
} } IBM Analytical Services; http://www.chips.ibm.com/services/asg
} }
} }
} }
} }
} }
} _____________________________________
}
} Sergey Ryazantsev Ph. D.
} Electron Microscopy
} UCLA School of Medicine
} Department of Biological Chemistry
} Box 951737
} Los Angeles, CA 90095-1737
}
} Phone: (310) 825-1144
} Pager: (310) 845-0248
} FAX (departmental): (310) 206-5272
} mailto:sryazant-at-ucla.edu
} http://www.bol.ucla.edu/~sryazant

Dr. Lawrence F. Allard
Senior Research Staff Member
High Temperature Materials Laboratory
Oak Ridge National Laboratory
1 Bethel Valley Road
Bldg. 4515, MS 6064
PO Box 2008
Oak Ridge, TN 37831-6064

423-574-4981
423-574-4913 Fax
l2a-at-ornl.gov



From daemon Mon Apr 03 07:53:59 2000



From: donald j marshall :      dmrelion-at-world.std.com
Date: Sun, 2 Apr 2000 21:34:13 -0400 (EDT)
Subject: Re: Operator Horror Stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


What in the world is an Anglepoise lamp? Is this the same as a "Gooseneck"?

Don Marshall


} From Microscopy-request-at-sparc5.microscopy.com Sun Apr 2 17:01:37 2000
}
} -----------------------------------------------------------------------.
}
}
} I have a similar story to this one! A bright fellow from a senior
} University (I would embarrass them) had a dim TEM image, so rigged up
} an Anglepoise lamp to try and throw some more light on it!
}
} Keith Ryan
} Marine Biological Association
} Plymouth, UK
}

} -----------------------------------------------------------------------.
}
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} } To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
}
} } On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
}
} } -----------------------------------------------------------------------.
} } Humorous, but not quite horror:
}
}
} The respected PhD I once worked with on our old JEOL JSM-U3 scanner,
}
} who was having trouble making out the details of the image on the
} monitor, so grabbed a flashlight and shined it at the screen so he
} could see the image a little better... :-).
}
} Larry
}
} }
} }
} } Originally posted by: sryazant-at-ucla.edu
} }
} }
} }
} }
} } It is not "horror" story, but a sort of... Many years ago the
} colleague
} } from friendly Lab visited me with great project. The idea was
} simply and
} } beautiful. She argues that because the image in the scope (TEM) is
} green
} } (green fluorescence of the screen), she wants to modify the sample
} (I
} } forgot what, some protein, I believe) by red-fluorescent dye to be
} able to
} } see on the screen of the electron microscope the "double-staining":
} red on
} } the green background. No comments...
} }
} } Sergey
} }
} } } Date: Thu, 30 Mar 2000 09:17:56 -0500
} } } From: "anderron-at-us.ibm.com"-at-sparc5.microscopy.com
} } } Subject: Operator Horror Stories
} } } To: microscopy-at-sparc5.microscopy.com
} } } Importance: Normal
} } } X-MIMETrack: Serialize by Router on D01ML065/01/M/IBM(Release
} 5.0.2b
} } (Intl)|18
} } } January 2000) at 30/03/2000 09:18:01
} } }
} }
} } ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} } } To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
}
} } } On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
}
} }
} } -----------------------------------------------------------------------.
} } }
} } }
} } }
} } } Alan Fox, in a response to the Analytical TEM string, cautioned
} the
} } } question-raiser to beware of people who abuse instruments.
} Perhaps Nestor
} } } will forgive a little microscopy related humor and allow us to
} start a
} } } string on "Operator Horror Stories." Here's ours:
} } }
} } } The "ham-fisted user" reference made us chuckle/cringe with the
} memory of
} } } a guest "microscopist" in our lab who hauled himself (220 pounds
} or so) out
} } } of his seat by pulling on the half-inserted specimen rod, bending
} it about
} } } 20 degrees or so!
} } }
} } } Henceforth, when we saw him in the hall (he never came into the
} scope room
} } } again), we referred to him as "Conan the Microscopist"!
} } }
} } }
} } }
} } } Ron Anderson, IBM, Hopewell Jct., New York, USA.
} anderron-at-us.ibm.com
}
} } }
} } } IBM Analytical Services; http://www.chips.ibm.com/services/asg
} } }
} } }
} } }
} } }
} } }
} } _____________________________________
} }
} } Sergey Ryazantsev Ph. D.
} } Electron Microscopy
} } UCLA School of Medicine
} } Department of Biological Chemistry
} } Box 951737
} } Los Angeles, CA 90095-1737
} }
} } Phone: (310) 825-1144
} } Pager: (310) 845-0248
} } FAX (departmental): (310) 206-5272
} } mailto:sryazant-at-ucla.edu
} } http://www.bol.ucla.edu/~sryazant
}
} Dr. Lawrence F. Allard
} Senior Research Staff Member
} High Temperature Materials Laboratory
} Oak Ridge National Laboratory
} 1 Bethel Valley Road
} Bldg. 4515, MS 6064
} PO Box 2008
} Oak Ridge, TN 37831-6064
}
} 423-574-4981
} 423-574-4913 Fax
} l2a-at-ornl.gov
}
}



From daemon Mon Apr 03 07:54:10 2000



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Mon, 3 Apr 2000 13:33:48 +0100 (GMT Daylight Time)
Subject: Re: Horror Stories

Contents Retrieved from Microscopy Listserver Archives
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SEE
http://www.anglepoise.co.uk/



Date sent: Sun, 2 Apr 2000 21:34:13 -0400 (EDT)
} From: donald j marshall {dmrelion-at-world.std.com}
To: KPR-at-wpo.nerc.ac.uk


I heard this one at a conference last week. A PhD student
was caught sawing a knob off a FE SEM. Apparently it was
pushing against his desk so decided to saw it off and was
going to glue it on at a 45 degree angle!

Dave




On Sat, 1 Apr 2000 06:54:53 -0000 Lou Solebello
{microls1297-at-mindspring.com} wrote:

} ------------------------------------------------------------------------
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} -----------------------------------------------------------------------.
}
}
} My story is not as much of a horror as it is embarassing. Several years
} back I received a package of samples from a regular client without any paper
} work describing what the samples were. This is not unusual since we
} frequently recieve unknowns. I proceeded to open the package and was
} abruptly assaulted with an extremely strong odor of bannanas. Apparantly one
} of the samples was a vial of concentrated bannana flavoring. It was months
} (almost a year) before the odor completely disappeared from my office, and I
} was cajoled about it frequently. I by the way, hate bannanas.............
}
} Lou Solebello
} -----Original Message-----
} } From: L R MELSEN {lmelsen-at-emory.edu}
} To: Microscopy-at-sparc5.microscopy.com {Microscopy-at-sparc5.microscopy.com}
} Date: Friday, March 31, 2000 9:26 PM
} Subject: Storys
}
}
} } ------------------------------------------------------------------------
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} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } We had a cousin of Conan , who in his addled age, after inserting the
} } injector tip into the stage of our EM400 could not find his grid in the
} } microscope; he had dropped it on the floor. Of course the tip must have
} } fallen off in the stage, so he promptly took a second tip and properly
} } inserted the second tip on top of the first. Still no grid could be
} } found. Ah ha, I will leave a polite note explaining the problem. It read
} } as follows:
} } " Please check the microscope, I had great difficulty inserting my
} } specimens last evening."
} } Philips kindly replaced the bulk of the stage just so they could keep
} } the original for the museum of what not to do.
} }
} }
}
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"



From daemon Mon Apr 03 08:09:35 2000



From: Julian Smith III :      smithj-at-Winthrop.edu
Date: Mon, 3 Apr 2000 07:56:46 -0500
Subject: Re: SEM - Coating

Contents Retrieved from Microscopy Listserver Archives
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It does't have oxygen in it--with air, 02- is produced and accelerated at
your specimens, eroding them. When done intentionally, this is called
"plasma ashing," i believe. If your specimens look very smooth, this may
be why.
Argon produces Ar2+, which, if your coater is set up right, is accelerated
at the gold target.
JSIII

} Hello,
}
} I would like to know why they use Argon in the metal coating devices. At
} our laboratory, we don't use Argon, we use air. What is the advantage of
} using Argon ?
} De Pauw Bart
} Faculty of Veterinary Medicine
} Morphology
} Salisburylaan 133
} 9820 Merelbeke
} Belgium
} Phone : 0032(0)9 264.77.19
} Fax : 0032(0)9 264.77.90


Julian P.S. Smith III
Dept. of Biology
Winthrop University
Rock Hill, SC 29733
803-323-2111 x6427 (vox)
803-323-3448 (fax)




From daemon Mon Apr 03 17:47:39 2000



From: Van Osta, Peter [JanBe] :      PVOSTA-at-janbe.jnj.com
Date: Mon, 3 Apr 2000 15:50:19 +0200
Subject: Illumination Technologies lightsource to ZEISS micrscope

Contents Retrieved from Microscopy Listserver Archives
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Hi,

Has anyone foud a solution for the following problem, I am getting
desperate. There doesn't seem to be a ready-made solution available ?

We want to link an Illumination Technologies light-source to a ZEISS
microscope. We are trying to get the right information about the necessary
parts, but this seems to be non-trivial.

The following problems need a solution:

ZEISS Axioskop upright microscope and Illumination Technologies CF1000
ZEISS Axiovert 100M inverted microscope and Illumination Technologies 3900

In both cases we need a light guide and a fiber coupler to the microscope.
Both light sources are to be used for epifluorescence applications.

Sincerely yours,

Peter Van Osta, MD

Senior Scientist Medical Image Analysis
Biological Imaging Laboratory
Life Sciences Department I - 6065
Janssen Research Foundation
Turnhoutseweg 30
B-2340 Beerse
Belgium
Europe

tel.: +32 (0)14 60.60.65 (office hours, GMT+1)
fax.: +32 (0)14 60.57.88

email: pvosta-at-janbe.jnj.com

WWW: http://ourworld.compuserve.com/homepages/pvosta




From daemon Mon Apr 03 17:47:40 2000



From: Peggy Bisher :      peggy-at-research.nj.nec.com
Date: Mon, 3 Apr 2000 10:22:20 -0400
Subject: Microscope Horror Stories

Contents Retrieved from Microscopy Listserver Archives
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I heard this one from the Philip's engineers: A brand new Philips
microscope was being installed in a goverment laboratory. It was to be in
a state of the art, brand-new building. Everything was there, house
nitrogen, chilled water, etc. Even the darkroom was state of the art.
Well, when the in-house plumbers hooked up the water to the microscope they
weren't being very careful in their reading of the blueprint designs and
they had D-19 developer running through the EM instead of water. I'm not
sure, but I think they got a new microscope out of that blunder!


Margaret E. Bisher

NEC Research Institute
4 Independence Way
Princeton, NJ 08540.
Tel.: (609) 951-2629
Fax: (609) 951-2496
e-mail: peggy-at-research.nj.nec.com







From daemon Mon Apr 03 17:47:43 2000



From: Debby Sherman :      sherman-at-btny.purdue.edu
Date: Friday, March 31, 2000
Subject: Fwd: Operator Horror Stories

Contents Retrieved from Microscopy Listserver Archives
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An additional story which had the potential of being a real personnal horror story is as follows:

I worked for Humberto Fernandez-Moran at the University of Chicao many years ago. For those of you who are not familiar with the name, he was instrumental in developing the first diamond knives, producing the first pointed filaments for routine use and construction of first cryo-TEM using liquid helium cooled lenses. It was a very interesting place for a young budding microscopist at the time!

Dr. Moran had a large scar on his nose. He said it was from the removal of a cancerous skin area. He claimed to have gotten the malignancy in that location due to using electron microscopes in the late 40's without the benefit of lead glass windows. They used to press their noses against the window when concentrating on the relatively dim image projected by those early instruments. I wonder if other early EM researchers eventually developed cancer which might be related to similar research experiences.

As it turned out, Dr. Moran lived a long life, although not without controversy through the years. He had a very unusual life history and was also a brilliant but erratic person to interact with....somewhat akin to what Bobby Knight is to basketball!

Debby
--------------------------------------


Many years ago we had a Siemens EM-101. This was a relatively new
microscope and beautifully machined with German precision. The camera
chamber was a work of art. Each film plate (we used glass back then) was
encased in it's own light-tight cassette. The camera would move a film
cassette into position for exposure and pull off the cassette cover. The
operator would then expose the film and move the casette into a drop box.
The cassette cover would be pushed closed in the process.
This camera worked almost flawlessly. On occassion, an operator, when
loading the camera, would put the cassettes in on an angle. When you went
to take the first image, the cassette would jam and not move into position.
All that was necessary to unjam was to break vacuum, giggle the cassette
with your hand to straighten it and then repump the camera chamber and get
back to work...a 10 minute process as most.
We had one operator who thought she knew it all. She was working on the
microscope one evening and the camera jammed. Her solution was to take out
the camera and take it apart...screw by screw and spring by spring. She
ended up with an incredible heap of parts and of course had no clue as to
how to put it back together. Neither did the Siemens service engineers.
They had never seen one apart! They had to find another camera (not easy
in those days with relatively few of these instruments around), take it
apart..carefully marking where each piece came from...and then reassembled
each camera simultaneously. It took hours!!
Moral of the story...NO ONE fixed ANYTHING without permission of the lab
personnel!!



Debby Sherman, Manager Phone: 765-494-6666
Microscopy Center in Agriculture FAX: 765-494-5896
Dept. of Botany & Plant Pathology E-mail: sherman-at-btny.purdue.edu
Purdue University
1057 Whistler Building
West Lafayette, IN 47907-1057







From daemon Mon Apr 03 17:47:44 2000



From: Hendrik O. Colijn :      colijn.1-at-osu.edu
Date: Mon, 03 Apr 2000 09:10:59 -0400
Subject: UHV compatibility of phenolic resins

Contents Retrieved from Microscopy Listserver Archives
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Hi all,

I have just been asked to do auger analysis on a sample mounted in phenolic
mounting resin. Normally I require that these samples be demounted for UHV
compatibility but this person doesn't wish to do so.

Does anyone have any experience with putting phenolic mounting material in
a UHV environment? Will I be seeing carbon on all my samples for the next
few years?

Thanks,
Henk

Hendrik O. Colijn colijn.1-at-osu.edu
Campus Electron Optics Facility Ohio State University
(614) 292-0674 http://web.ceof.ohio-state.edu
Murphy's Law: "If anything can go wrong, it will."
Commentary: "Murphy was an optimist."



From daemon Mon Apr 03 17:47:45 2000



From: Barbara Foster :      mme-at-map.com
Date: Mon, 03 Apr 2000 12:22:45 -0700
Subject: Re: Illumination Technologies lightsource to ZEISS micrscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Peter,

There is a new system which sounds like it a good fit for your application.
It is a liquid light guide with coupler, lamp (long lived HBO) and power
supply from EFOS. Contact: Allan Firhoj PH: 905-812-4302 Email:
AllanF-at-EFOS.com URL: www.efos.com

Caveat: MME has no financial interest in this product.

Best regards,
Barbara Foster
Consortium President
Microscopy/Microscopy Education ...Educating microscopists for greater
productivity.

125 Paridon Street Suite 102 Springfield, MA 01118
PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com
Visit our web site {http://www.MME-Microscopy.com/education}
******************************************************


At 03:50 PM 4/3/00 +0200, Van Osta, Peter [JanBe] wrote:
} ------------------------------------------------------------------------
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From daemon Mon Apr 03 17:47:47 2000



From: beth-at-dogwood.botany.uga.edu (Beth Richardson)
Date: Mon, 3 Apr 2000 13:15:39 -0500
Subject: Operator Horror Stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I love reading the "horror stories". Here's a personal one:

When I was a grad student I did a CPD run with a fellow grad student JT. I
worked on plant pathogens and JT worked on chick embryo hearts so we had
little pieces of leaf tissue and tiny chick hearts to dry. When the run was
finished I couldn't get the lid off the CPD (it was a twist type). JT
volunteered to muscle it off and when she did the lid shot passed her head
with a boom and hit the ceiling. She had a grazing wound on her forehead
but was otherwise ok. We both burst into fits of nervous laughter...we both
knew she was so lucky not to be seriously injured. Then we looked in the
CPD and saw that all the lids had blown off the little white sample
containers. We howled with laughter when we got down on our hands and knees
to search the floor for the tiny hearts and leaf pieces. The CPD was fine,
the samples were fine, and surprisingly we both graduated.

Beth




From daemon Mon Apr 03 17:47:50 2000



From: Gerald Harrison :      jerry-at-biochem.dental.upenn.edu
Date: Mon, 03 Apr 2000 14:04:03 -0400
Subject: Re: Operator Horror Stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello to Beth and all,

} [Beth wrote:]
} I love reading the "horror stories". Here's a personal one:

I do, also. Although I have not posted to this List often (last
time was for advice in purchasing a confocal which was very helpful and we
are very pleased with the purchase), I've wanted to chime in and say how
enjoyable it is to read these 'stories'.
I think there is a very relevant component to these stories in
that they help us be more aware of how easy it is for some very unusual,
silly and sometimes dangerous things to happen when you thought such a
thing was impossible if it even crossed your mind at all.

Sorry, but thankfully, (very thankfully) I have no horror stories,
yet... Having said that, uh oh...

Gerald Harrison
================
} [Beth continued]
} When I was a grad student I did a CPD run with a fellow grad student JT. I
} worked on plant pathogens and JT worked on chick embryo hearts so we had
} little pieces of leaf tissue and tiny chick hearts to dry. When the run was
} finished I couldn't get the lid off the CPD (it was a twist type). JT
} volunteered to muscle it off and when she did the lid shot passed her head
} with a boom and hit the ceiling. She had a grazing wound on her forehead
} but was otherwise ok. We both burst into fits of nervous laughter...we both
} knew she was so lucky not to be seriously injured. Then we looked in the
} CPD and saw that all the lids had blown off the little white sample
} containers. We howled with laughter when we got down on our hands and knees
} to search the floor for the tiny hearts and leaf pieces. The CPD was fine,
} the samples were fine, and surprisingly we both graduated.
}
} Beth
}




From daemon Mon Apr 03 17:47:51 2000



From: David R Hull :      David.R.Hull-at-grc.nasa.gov
Date: Mon, 3 Apr 2000 14:12:21 -0400
Subject: Re: Microscope Horror Stories

Contents Retrieved from Microscopy Listserver Archives
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I can't even begin to think of why you would have D-19 developer in a
pressurized pipe system? Did they process 1000's of negatives a day?


At 10:22 AM -0400 4/3/00, Peggy Bisher wrote:
} ------------------------------------------------------------------------
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David R. Hull
NASA Glenn Research Center at Lewis Field
Advanced Metallics Branch
Mail Stop 49-1
21000 Brookpark Road
Cleveland, OH 44135

(216) 433-3281
fax (216)977- 7132
david.r.hull-at-grc.nasa.gov



From daemon Mon Apr 03 17:47:51 2000



From: Peggy Bisher :      peggy-at-research.nj.nec.com
Date: Mon, 3 Apr 2000 14:40:34 -0400
Subject: Re: Microscope Horror Stories

Contents Retrieved from Microscopy Listserver Archives
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The Philip's guy's did not give me such a clear answer. I can only
assume that something stupid happened, like D-19 was put in the closed loop
system of the Haskris Chiller, instead of the normal water. I do know that
this darkroom was to service a large EM suite so perhaps there was this big
tank of D-19 made up and they (the plumbers) grabbed it and used it. It
does sound incredibly stupid, that's for sure.


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Margaret E. Bisher

NEC Research Institute
4 Independence Way
Princeton, NJ 08540.
Tel.: (609) 951-2629
Fax: (609) 951-2496
e-mail: peggy-at-research.nj.nec.com







From daemon Mon Apr 03 17:47:53 2000



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Mon, 3 Apr 2000 08:59:07 -1000 (HST)
Subject: Microscopy horror stories

Contents Retrieved from Microscopy Listserver Archives
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These stories are a giggle, and also food for thought!

I would like to collect them for a future Net Notes, part of the News and
Commentary section of Microscopy and Microanalysis. So keep them
coming! I may have to edit them down to a reasonable number, and it would
be a few months before they appeared, but they are too good to pass up.

Aloha,
Tina

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************



From daemon Mon Apr 03 17:47:57 2000



From: William Tivol :      tivol-at-wadsworth.org
Date: Mon, 03 Apr 2000 16:24:06 -0400
Subject: Re: Horror Inspection

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Lou Ann Miller wrote:

} We had a CAP inspection, the officers were from another hospital in the state.
}
}
} The question I was expected to reason and answer with a straight face was:
}
}
} * What other protective measures besides standing behind a lead
} wall do you take when you put the glass slide into the TEM, to
} protect yourself from the flying electrons???
}

Dear Lou Ann,
In addition to the obvious ludicrous aspects to the question, the
use of a lead shield to protect oneself against "flying electrons" is one
of the worst things to do. One should use a low-Z absorber for elec-
trons to reduce brehmsstrahlung x-rays, then, if desired, put lead
between oneself and the low-Z shield to absorb those x-rays which
are produced.
Yours,
Bill Tivol



From daemon Mon Apr 03 17:47:58 2000



From: William Tivol :      tivol-at-wadsworth.org
Date: Mon, 03 Apr 2000 16:35:30 -0400
Subject: Re: Operator Horror Stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Damian wrote:

} I heard that one years ago. Also one that supposedly happened at Univ.
} Wisconsin. Someone apparently cleaned a scope's internal parts in acetone
} and started pumping. Before it was fully pumped down, they turned on the
} filament. Bang! Nice story but could it possibly happen? I would think
} that any acetone residue would evaporate very quickly or is there some
} other phenomenon here relating to ratio of gases?

Dear Damian,
There must have been a lot of residue in the scope to have caused

an explosion. Not only would one need both acetone and oxygen in a
suitable ratio, there would have to be enough so that the heat of combustion
from one acetone molecule oxidising would cause other molecules to
react, otherwise there would just (!) be a slow burning of the vapors. In
addition, the acetone/oxygen ratio would be determined by both the
amount of residual acetone and the relative pumping speeds for acetone
and oxygen. No doubt there are people on this list who would know
the pump speed ratio, so one could calculate the expected behavior of
the acetone in the column for various values of the relevant parameters.
Yours,
Bill Tivol




From daemon Mon Apr 03 17:48:00 2000



From: COURYHOUSE-at-aol.com
Date: Mon, 3 Apr 2000 17:43:21 EDT
Subject: Re: Microscopy horror stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


If no one has any objections I would also like to post these on our upcoming
section on microscopy for our museum's web site when it is finished.
Ed Sharpe archivist for SMECC



From daemon Mon Apr 03 17:48:01 2000



From: Kriho, Virginia :      Vkriho-at-psych.uic.edu
Date: Mon, 3 Apr 2000 16:45:46 -0500
Subject: no more horror stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I don't mean to be a drag, but enough of these "horror stories". My email
is clogged with these stories. I only want the facts, Sir. Thanks.



From daemon Mon Apr 03 17:48:02 2000



From: David Knecht :      knecht-at-uconnvm.uconn.edu
Date: Mon, 3 Apr 2000 18:09:49 -0400
Subject: extended focus software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I remember somewhere in the past a discussion of software for
creating extended focus images (I think that is the correct term), in
which a series of transmitted light images in different focal planes
are combined in order to remove the out of focus information and show
all parts of the image in focus. Can someone point to software that
carries out this function? Thanks- Dave
--

************************************************************
"Home of the 2000 NCAA Women's Basketball Champions"

Dr. David Knecht
Department of Molecular and Cell Biology
University of Connecticut
75 N. Eagleville Rd. U-125
Storrs, CT 06269
Knecht-at-uconnvm.uconn.edu
860-486-2200 860-486-4331 (fax)
home page: http://www.sp.uconn.edu/~mcbstaff/knecht/knecht.html
************************************************************



From daemon Mon Apr 03 18:28:05 2000



From: =shAf= :      mshaf-at-darkwing.uoregon.edu
Date: Mon, 3 Apr 2000 18:10:46 -0500
Subject: SEM: gun emission and biasing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I've just noticed behavior which is different for my 2 e-beam
instruments. If I bias my microprobe's gun for less emission, the
beam current measured in a specimen faraday cup also goes down.
However, if I bias my SEM's gun for less emission, the beam current
(measured similarly) goes up(???). This may mean simply a shallower
optic angle for the SEM and the anode allowing more electrons to pass,
but I thought I'd throw the observation out there.

cheerios, shAf

{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {}
Michael Shaffer, R.A. - ICQ 210524
Geological Science's Electron Probe Facility - University of Oregon
mshaf-at-darkwing.uoregon.edu - http://darkwing.uoregon.edu/~mshaf/




From daemon Mon Apr 03 18:28:05 2000



From: Praveena Bhaskara :      bubbyp-at-hotmail.com
Date: Mon, 3 Apr 2000 18:09:33 -0500
Subject: ultramicrotomy: PP wires

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hi everyone!
I'm new to this microscopy society and 'am having trouble already!!To make
it worse everyone is sending me panic stories!! Just kidding!
I'm working on some poly-propylene wires. I need to take some TEM images but
I'm having trouble at the first stage itself...ultramicrotomy. You see I
need to fuse two PP wires and image the interface. Now the problem is, when
I'm cutting it with the microtome, the interface just breaks off!!Right now
I'm using a epoxy-hardner ratio of 10:4. will changing the proportions help?
I'm thinking of using a diamond knife also. Does anyone have any bright
ideas?!!Help me out on this!!
Praveena
______________________________________________________
Get Your Private, Free Email at http://www.hotmail.com




From daemon Mon Apr 03 18:58:17 2000



From: DrJohnRuss-at-aol.com
Date: Mon, 3 Apr 2000 19:49:27 EDT
Subject: Re: extended focus software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



In a message dated 4/3/00 6:23:17 PM, knecht-at-uconnvm.uconn.edu writes:

} I remember somewhere in the past a discussion of software for
} creating extended focus images (I think that is the correct term), in
} which a series of transmitted light images in different focal planes
} are combined in order to remove the out of focus information and show
} all parts of the image in focus. Can someone point to software that
} carries out this function? Thanks- Dave

That function is performed in The Image Processing Tool Kit
(http://members.aol.com/ImagProcTK/) by examining each pixel location in the
two (or more) images and calculating the local variance in a 5 pixel wide
circle. The pixel value that has the largest variance is kept, the rationale
being that it has the most abrupt local variations and is likely to be in the
sharpest focus. The method works pretty well for most microscope images where
the magnification remains constant, but not so well for macroscopic images
where the out-of-focus portions of the image are also shifted due to changes
in focal length. It also doesn't handle cases with specular reflections to
well. But it should be OK for your transmitted light case. There are other
approaches in some software packages that use a simple high pass filter (e.g.
a Laplacian) but this seems from my experiments to be no faster and more
noise sensitive.



From daemon Mon Apr 03 19:51:34 2000



From: Markham Jan-AFP042 :      AFP042-at-email.mot.com
Date: Mon, 3 Apr 2000 17:23:13 -0700
Subject: no more horror stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Lighten up - the delete button is easy enough to use :-) I often feel the
same way about all the bio postings and these are a lot more fun to read,
besides having some educational value.

Jan Markham
Motorola FPDD
7700 South River Parkway, FPD22
Tempe, AZ 85284
Ph: (480)755-5509
FAX: (480)755-5115
Email: afp042-at-email.mot.com


-----Original Message-----
} From: Kriho, Virginia [mailto:Vkriho-at-psych.uic.edu]
Sent: Monday, April 03, 2000 2:46 PM
To: 'List Server'


I don't mean to be a drag, but enough of these "horror stories". My email
is clogged with these stories. I only want the facts, Sir. Thanks.



From daemon Mon Apr 03 19:51:34 2000



From: Harrison :      tuttle-at-home.com
Date: Mon, 3 Apr 2000 17:31:00 -0700
Subject: Re: Microscope Horror Stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Here's another one. A customer on the west coast had a bottle of N2 that
was used to vent their TEM . They also used the same bottle to agitate their
developer. One day someone left the N2 on in the developer and it ran out.
You can guess what happened next, the TEM was vented with D-19.

Dave Harrison




From daemon Mon Apr 03 21:46:52 2000



From: Don Grimes :      microtoday-at-mindspring.com
Date: Mon, 3 Apr 2000 22:30:15 -0500
Subject: Horror Stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Group,
Just a note to advise that I intend to publish a summary of these "horror"
stories in an upcoming issue of Microscopy Today. For those of you who do
not receive our publication, including those overseas, I will provide a copy
of the summary - at no charge.
To the authors (past, current and future), should you not wish to have your
comment included on our summary, kindly advise by return email and I will
insure that it does not happen.
Best to all,
Don Grimes, Microscopy Today



From daemon Mon Apr 03 22:26:58 2000



From: Sally Stowe :      STOWE-at-rsbs.anu.edu.au
Date: Tue, 04 Apr 2000 13:18:40 +1000
Subject: Re: Horror Stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all -
Ditto for the Australian EM Newsletter. (Somebody has volunteered to put
together a choice selection.).
and one from us...
-the guy who asked how long to wash between dehydration steps

and from another place a long time ago-
-the Professor who offered to pay the electron microscopist by giving him
some oil immersion objectives for the TEM.








Dr Sally Stowe
Facility Coordinator, ANU EMU
Box 475 Canberra ACT 2601
AUSTRALIA
stowe-at-rsbs.anu.edu.au fax 61 (0)2 6249 3218 or 6279 8525

} } } "Don Grimes" {microtoday-at-mindspring.com} 04/04/00 01:30pm } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Group,
Just a note to advise that I intend to publish a summary of these "horror"
stories in an upcoming issue of Microscopy Today. For those of you who do
not receive our publication, including those overseas, I will provide a
copy
of the summary - at no charge.
To the authors (past, current and future), should you not wish to have
your
comment included on our summary, kindly advise by return email and I will
insure that it does not happen.
Best to all,
Don Grimes, Microscopy Today




From daemon Mon Apr 03 22:27:00 2000



From: Ronald Vane :      RVaneXEI-at-concentric.net
Date: Mon, 3 Apr 2000 20:19:04 -0700
Subject: Re: Horror Stories rules

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


These stories are fun, but we are spending a lot e-mail on them.

I suggest these rules:
1. The story should have happened to you or your lab.
Don't repeat "Urban Legends" like "acetone vapor explodes microscope".

2. Don't comment on the stories. Assume everyone understands the problem or
science behind the story.

Now I will duck behind the computer.

Ron Vane
XEI Scientific



-



From daemon Mon Apr 03 22:50:50 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 03 Apr 2000 20:38:22 -0500
Subject: Re: extended focus software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I know that Soft Imaging's AnalySIS with EFI does this. It takes a bunch
of images that are focused at various points and combines them into one
image which is totally in focus.

gary g.



At 05:09 PM 4/3/00 , you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I remember somewhere in the past a discussion of software for creating extended focus images (I think that is the correct term), in which a series of transmitted light images in different focal planes are combined in order to remove the out of focus information and show all parts of the image in focus. Can someone point to software that carries out this function? Thanks- Dave
} --
}
} ************************************************************
} "Home of the 2000 NCAA Women's Basketball Champions"
}
} Dr. David Knecht
} Department of Molecular and Cell Biology
} University of Connecticut
} 75 N. Eagleville Rd. U-125
} Storrs, CT 06269
} Knecht-at-uconnvm.uconn.edu
} 860-486-2200 860-486-4331 (fax)
} home page: http://www.sp.uconn.edu/~mcbstaff/knecht/knecht.html
} ************************************************************



From daemon Tue Apr 04 15:00:05 2000



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Mon, 03 Apr 2000 21:54:53 -0700
Subject: RE: no more horror stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I strongly believe that many of us are able to extract facts even from
"horror stories". Fact, what is that? In my point of view, the "horror
stories" are pure extract from people's experience showing to us how manage
EM facilities properly. I was surprised to know, for instance, that
somebody was able to bent sample-holder standing up from the chair. I will
include special topic about that case in my instructions for users now. If
you don't like the way people share their experience, you may set filter tn
the word "horror" in the E.mail program and direct those messages into the
trash-folder. A little bit humor, here at ListServer is not bad.

Best wishes, Sergey.

P.S. It is not bad tradition, again, at ListServer to sign the messages.

} Date: Mon, 03 Apr 2000 16:45:46 -0500
} From: "Kriho, Virginia" {Vkriho-at-psych.uic.edu}
} Subject: no more horror stories
} To: 'List Server' {microscopy-at-sparc5.microscopy.com}
} X-Mailer: Internet Mail Service (5.5.2448.0)
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu
http://www.bol.ucla.edu/~sryazant




From daemon Tue Apr 04 15:00:07 2000



From: Mel Dickson :      m.dickson-at-unsw.edu.au
Date: Tue, 04 Apr 2000 15:41:31 +1000
Subject: Lessons in horror stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have been responsible for a multi-user facility since 1970.

It has always run on the basis that anyone can walk in and learn to use the
equipment. So we never have fights about access or possession.

We now have around 300 users of which about 150 start fresh each year.

How is it our machines are not all wrecks?

The FIRST lesson teaches two golden rules:

1. NEVER use force on any control

2. ALWAYS ask for help as soon as you dont understand what is happening.

Our few bad incidents have occurred because these rules were neglected.

When a user who is in trouble calls me in to sort it out I try very hard to
always be cheerful and positive no matter how stupid they have been. I
think if I am cheerful they will call me in next time they have a problem.
If I am furious with them they will maybe try to hide their blunder, or
worse, try to fix it themselves.

Of course, I must apply the rule to myself. When I dont understand what is
happening, it is time to call the service engineer!


Dr. Mel Dickson,
Deputy Director, The Electron Microscope Unit,
Adjunct Associate Professor, School of Microbiology & Immunology
The University of New South Wales
UNSW SYDNEY 2052
Australia.
Phone +612 9385 6383 Fax +612 9385 6400



From daemon Tue Apr 04 15:00:08 2000



From: Rosemary White :      Rosemary.White-at-pi.csiro.au
Date: Tue, 4 Apr 2000 16:19:33 +1000
Subject: Re: Microscope Horror Stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Well, after reading Peggy Bisher's story, I couldn't help but add another
along similar lines.

About 20-odd years ago, a prestigous institution purchased a state of the
art new TEM. The main body came in a very large wooden container and was
unloaded onto the loading dock at the back of the building. It sat there
for quite a while, because it was too heavy to move with the regular
forklift, and I think the lab still needed a few final things to be
finished off. Anyway, one day, someone decided that they were going to
move the TEM in, and loaded it on the forklift. About half way to the lab,
the TEM started oscillating back and forth on the forklift - it wasn't
strapped on securely, and an eyewitness said he just stood there and
watched this thing slowly crash to the floor on its side. Not much use
rushing in and getting crushed by a few 100 kilos of metal.

I'm not sure it ever worked properly, the camera was smashed and a few
other things too. The workshop had to retool all the smashed bits as best
they could.

Amazing how many ways there are to destroy precision instruments.

cheers,

Rosemary White
Microscopy Centre
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia

phone 61-2-6246 5475
fax 61-2-6246 5000
email r.white-at-pi.csiro.au




From daemon Tue Apr 04 15:00:21 2000



From: Ron Doole :      ron.doole-at-materials.oxford.ac.uk
Date: Tue, 4 Apr 2000 09:39:37 +0100 (BST)
Subject: preventing horrors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All,

I agree with Mel Dickson whole heartedly. I learnt many years ago
that to bawl out a user for being stupid results in silence. The only way
to find out what really happens is to be as helpful as possible whatever
the situation. Rant and rave to let off steam later, in the privacy of
another room.
To this end I welcome the horror stories. It is better to tell
users stories of other incidents to get them to be careful and to
think about the situation. They are more willing to ask a `stupid'
question without embarrasment if they are aware of the mistakes that have
been made. If this prevents them making furhter mistakes then I'm all for
it.

Ron

===========================================================================
Mr. Ron Doole e-mail ron.doole-at-materials.ox.ac.uk
Department of Materials, phone +44 (0) 1865 273701
University of Oxford, fax +44 (0) 1865 283333
Parks Road.
Oxford. OX1 3PH. UK.
============================================================================





From daemon Tue Apr 04 15:00:22 2000



From: John Runions :      cjr41-at-cam.ac.uk
Date: Tue, 04 Apr 2000 09:39:30 +0100
Subject: Re: no more horror stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


What Jan is saying is that `"bio" postings have no educational value ; )

Markham Jan-AFP042 wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Lighten up - the delete button is easy enough to use :-) I often feel the
} same way about all the bio postings and these are a lot more fun to read,
} besides having some educational value.
}
} Jan Markham
} Motorola FPDD
} 7700 South River Parkway, FPD22
} Tempe, AZ 85284
} Ph: (480)755-5509
} FAX: (480)755-5115
} Email: afp042-at-email.mot.com
}
} -----Original Message-----
} } From: Kriho, Virginia [mailto:Vkriho-at-psych.uic.edu]
} Sent: Monday, April 03, 2000 2:46 PM
} To: 'List Server'
} Subject: no more horror stories
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} I don't mean to be a drag, but enough of these "horror stories". My email
} is clogged with these stories. I only want the facts, Sir. Thanks.

--
C. John Runions, Ph. D.
Department of Plant Sciences
University of Cambridge
Downing St.
Cambridge
UK

email cjr41-at-cam.ac.uk
phone (01223) 529 249




From daemon Tue Apr 04 15:00:23 2000



From: Arnold Pizzey :      a.pizzey-at-ucl.ac.uk
Date: Tue, 04 Apr 2000 09:51:50 +0100
Subject: Re: extended focus software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


}
}
} I remember somewhere in the past a discussion of software for
} creating extended focus images (I think that is the correct term), in
} which a series of transmitted light images in different focal planes
} are combined in order to remove the out of focus information and show
} all parts of the image in focus. Can someone point to software that
} carries out this function? Thanks- Dave
} --
}
}
Greetings David,

Try Auto-montage (http://www.synbiosis.com/syncroscopy/am.asp)

If you contact them they will send you a demonstrtion CD of this product.

regards

Arnold

_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/
Arnold Richard Pizzey
Department of Haematology
Royal Free and University College London Medical School
98 Chenies Mews
London WC1E 6HX
U.K

voice: +44 0207-209-6234
Fax: +44 0207-209-6222
email: a.pizzey-at-ucl.ac.uk
_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/



From daemon Tue Apr 04 15:00:35 2000



From: Rosemary White :      Rosemary.White-at-pi.csiro.au
Date: Tue, 4 Apr 2000 19:45:20 +1000
Subject: Re: extended focus software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Dave,

There are a couple of options. Autoquant will do this, as will modules
from Soft Imaging Systems. I think some Zeiss software can do this too, as
long as there is no lateral shift in sequential images (as is found in some
dissecting scopes). These are packages we've tried, there may well be more.

cheers,
Rsoemary


Rosemary White
Microscopy Centre
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia

phone 61-2-6246 5475
fax 61-2-6246 5000
email r.white-at-pi.csiro.au




From daemon Tue Apr 04 15:00:37 2000



From: TQ23TEAM-at-LHT.DLH.DE
Date: Tue, 4 Apr 2000 11:55:12 +0200
Subject: AW: extended focus software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Dave,
you may have a look on the SIS (Soft Imaging Software) homepage. They offer
an EFI -modul.

http://www.soft-imaging.de/

In our metallographic labority, we use the EFI-module for one year and we
are very satisfied in working with it.

Hope ths answer will help.

Best regards
Bernd Schweisfurth
Lufthansa Technik
Hamburg / Germany
} ----------
} Von: David Knecht[SMTP:knecht-at-uconnvm.uconn.edu]
} Gesendet: Dienstag, 4. April 2000 00:09
} An: microscopy-at-sparc5.microscopy.com
} Betreff: extended focus software
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I remember somewhere in the past a discussion of software for
} creating extended focus images (I think that is the correct term), in
} which a series of transmitted light images in different focal planes
} are combined in order to remove the out of focus information and show
} all parts of the image in focus. Can someone point to software that
} carries out this function? Thanks- Dave
} --
}
} ************************************************************
} "Home of the 2000 NCAA Women's Basketball Champions"
}
} Dr. David Knecht
} Department of Molecular and Cell Biology
} University of Connecticut
} 75 N. Eagleville Rd. U-125
} Storrs, CT 06269
} Knecht-at-uconnvm.uconn.edu
} 860-486-2200 860-486-4331 (fax)
} home page: http://www.sp.uconn.edu/~mcbstaff/knecht/knecht.html
} ************************************************************
}



From daemon Tue Apr 04 15:00:39 2000



From: Molecular Biology Lab - Dept of Botany :      paam-at-rs.uovs.ac.za
Date: Tue, 4 Apr 2000 12:42:15 GMT2
Subject: Immunogold labeling background

Contents Retrieved from Microscopy Listserver Archives
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I am trying to localize a beta-1,3-glucanases gene expression after Russian
wheat aphid infestation. However I'm getting heavy labeling in the chloroplasts.
Unexpected because no previous localization studies have shown glucanases
to be expressed in the chloroplasts. My question is: How can I conform that
the labeling I am getting is not background or artifacts of some sort??
I am not using osmium only uranyl acetate and lead citrate for the staining

Thank you for any assistance
Martin Wilding


Martin Wilding
Department Botany & Genetics
University of the Orange Free State
P.O. Box 339
Bloemfontein
9300
South Africa

Tel +2751 4012818
Fax +2751 4488772
Email paam-at-rs.uovs.ac.za



From daemon Tue Apr 04 15:00:40 2000



From: Everett Ramer :      Everett.Ramer-at-netl.doe.gov
Date: Tue, 04 Apr 2000 07:06:54 -0400
Subject: Re: extended focus software

Contents Retrieved from Microscopy Listserver Archives
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David,
I wrote a macro for Optimas that does this; however, you should be able to do this with just about any image processing software. You can see an article I wrote in Microscopy Today, February/March 1988 for specifics. I can email you a copy of the article if you want it.


Everett Ramer
National Energy Technology Laboratory
Pittsburgh, PA, USA
412-386-4920
ramer-at-netl.doe.gov

} } } David Knecht {knecht-at-uconnvm.uconn.edu} 04/03/00 06:09PM } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


I remember somewhere in the past a discussion of software for
creating extended focus images (I think that is the correct term), in
which a series of transmitted light images in different focal planes
are combined in order to remove the out of focus information and show
all parts of the image in focus. Can someone point to software that
carries out this function? Thanks- Dave
--

************************************************************
"Home of the 2000 NCAA Women's Basketball Champions"

Dr. David Knecht
Department of Molecular and Cell Biology
University of Connecticut
75 N. Eagleville Rd. U-125
Storrs, CT 06269
Knecht-at-uconnvm.uconn.edu
860-486-2200 860-486-4331 (fax)
home page: http://www.sp.uconn.edu/~mcbstaff/knecht/knecht.html
************************************************************




From daemon Tue Apr 04 15:00:44 2000



From: Don Grimes :      microtoday-at-mindspring.com
Date: Tue, 4 Apr 2000 08:07:30 -0500
Subject: Horror Stories

Contents Retrieved from Microscopy Listserver Archives
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Group,
As suggested by several of you, and in addition to publishing an edited
summary of the stories in Microscopy Today, I will put the summary on my web
site where it can be downloaded by any with an interest. I will advise when
it is done.
And, should Nestor decide to discourage further contributions, send comments
to me directly and I will see that they are included.
Regards to all,
Don Grimes, Microscopy Today



From daemon Tue Apr 04 15:00:49 2000



From: Malis, Tom :      malis-at-nrcan.gc.ca
Date: Tue, 4 Apr 2000 09:16:28 -0400
Subject: RE: ultramicrotomy: PP wires

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


One of the problems with many interfaces like yours, Praveena, is that there
is not a lot of adhesion across them. Thus mechanical sectioning of any
form places a stress on the interface and decohesion can occur. (In many
cases of sectioned thin films at this lab, we've ended up with two debonded
layers adhering to the epoxy but not to each other after sectioning, thus I
don't think playing with the hardener ratio will help much). A diamond
knife makes a much smoother 'cut' and thus reduces these stresses, as will a
lower knife angle (like 35 degrees). Sectioning parallel to an interface is
less stressful than perpendicular to it. If you have an inherently weak
interface, however, chances are pretty good that decohesion will occur. You
may increase your probability of finding a portion that is still together by
trimming to an oversized block face.

Good luck.

Tom Malis
Group Leader - Characterization
Materials Technology Laboratory
Natural Resources Canada (Govt. of Canada)
568 Booth St., Ottawa, Canada
ph. 613-992-2310
FAX 613-992-8735
email: malis-at-nrcan.gc.ca
----------
From: Praveena Bhaskara [SMTP:bubbyp-at-hotmail.com]
Sent: April 03, 2000 7:10 PM
To: Microscopy-at-sparc5.microscopy.com
Subject: ultramicrotomy: PP wires


------------------------------------------------------------------------
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-----------------------------------------------------------------------.



Hi everyone!
I'm new to this microscopy society and 'am having trouble
already!!To make
it worse everyone is sending me panic stories!! Just kidding!
I'm working on some poly-propylene wires. I need to take some TEM
images but
I'm having trouble at the first stage itself...ultramicrotomy. You
see I
need to fuse two PP wires and image the interface. Now the problem
is, when
I'm cutting it with the microtome, the interface just breaks
off!!Right now
I'm using a epoxy-hardner ratio of 10:4. will changing the
proportions help?
I'm thinking of using a diamond knife also. Does anyone have any
bright
ideas?!!Help me out on this!!
Praveena
______________________________________________________
Get Your Private, Free Email at http://www.hotmail.com





From daemon Tue Apr 04 15:00:49 2000



From: Donald Lovett :      lovett-at-tcnj.edu
Date: Tue, 4 Apr 2000 09:25:21 -0400 (EDT)
Subject: Microscope Horror Stories

Contents Retrieved from Microscopy Listserver Archives
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This is less a horror story than a sad tale. When I took over managing
the EM facility here, I ordered a new cylinder of CO2 for the CPD. When
it arrived, I pointed out to the delivery person that he made a mistake
and had not supplied a tank with a siphon tube. He replied that he
delivered what he had always delivered. I checked the shipping records,
and, indeed, my predecessor had used C02 from a tank without a siphon
tube--in other words, for a decade he never critically point dried
a single specimen, since only C02 gas would have entered the chamber!

Perhaps more than a few people may want check their cylinders. (In the
US, the proper cylinder typically has a red band painted at the top of the
cylinder and the words "w/ dip tube" stenciled on the side.)

DL

______________________________________________________________________
Donald L. Lovett e-mail: lovett-at-tcnj.edu
Assoc. Professor, Dept. of Biology voice: (609) 771-2876
P.O. Box 7718 fax: (609) 637-5118
The College of New Jersey
Ewing, NJ 08628-0718







From daemon Tue Apr 04 15:00:50 2000



From: Witold Zielinski :      WIZIEL-at-inmat.pw.edu.pl
Date: Tue, 4 Apr 2000 15:37:29 +0000
Subject: Operator Horror Story

Contents Retrieved from Microscopy Listserver Archives
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Hi all:
Many years ago when comunists were in power in Eastern Europe, thanks
to a great person who knew how to deal with them, we got a beautiful
pice of equipment, with all the possible stages. One of them - the
heating stage - was especially impressive. All the people were amazed
that in situ TEM heating experiment might be performed up to 1000
centigrade. Among them was a young scientist who was investigating
some processes in aluminum. Probably, impressed by 1000 centigrade he
forgot about melting temperature. So, the new stage was required. At
this time it was a real horror story since the price of the stage was
almost equal the price of the small car - Fiat 126.
Have a nice day,
Witold Z.







%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
Witold Zielinski, Ph.D.
Warsaw University of Technology
Department of Materials Science and Engineering
02-507 Warszawa, Woloska 141
POLAND

phone #: /48 22/ 660 84 41
fax #: /48 22/ 848 48 75



From daemon Tue Apr 04 15:00:51 2000



From: Peggy Bisher :      peggy-at-research.nj.nec.com
Date: Tue, 4 Apr 2000 09:51:02 -0400
Subject: Correction: Microscope Horror Stories

Contents Retrieved from Microscopy Listserver Archives
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Hi George,

You didn't read my tale too carefully. I said the "in-house plumbers" did
the error. The Philip's folks had nothing to do with this blunder. They
just told me the story because it involved one of their microscopes. Maybe
I should have just left out who told me the story. I was only trying to
give them the credit and not take it myself. I am sure all of our EM
service engineers have some horror stories that would top any we have told,
but are trying to be polite for all of our sakes.

}
} Peggy told one on the Philips folks:
}
} } ... new Philips microscope ... goverment laboratory. ... house
} } nitrogen, chilled water, etc. Even the darkroom was state of the
} } art. Well, when the in-house plumbers hooked up the water to the
} } microscope they weren't being very careful in their reading of the
} } blueprint designs and they had D-19 developer running through the
} } EM instead of water. I'm not sure, but I think they got a new
} } microscope out of that blunder!
}


Margaret E. Bisher

NEC Research Institute
4 Independence Way
Princeton, NJ 08540.
Tel.: (609) 951-2629
Fax: (609) 951-2496
e-mail: peggy-at-research.nj.nec.com







From daemon Tue Apr 04 15:00:52 2000



From: William Carmichael :      wcarmichael-at-madison.tec.wi.us
Date: Tue, 04 Apr 2000 08:51:58 -0500
Subject: Re. extended focus software

Contents Retrieved from Microscopy Listserver Archives
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We use the AnalySIS software from Soft Imaging Systems in our lab and it works great. It's very easy to use and the results are an image which is in focus from top to bottom.



_______________________________

Bill Carmichael
Electron Microscopy Faculty

Madison Area Technical College
3550 Anderson St.
Madison, WI 53704
608-243-4309

wcarmichael-at-madison.tec.wi.us
http://electron-microscopy.madison.tec.wi.us




From daemon Tue Apr 04 15:00:53 2000



From: Stephen McCartney :      stmccart-at-vt.edu
Date: Tue, 04 Apr 2000 09:57:13 -0400
Subject: ultramicrotomy: PP wires

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Praveena wrote:

Hi everyone!
I'm new to this microscopy society and 'am having trouble already!!To make
it worse everyone is sending me panic stories!! Just kidding!
I'm working on some poly-propylene wires. I need to take some TEM images but
I'm having trouble at the first stage itself...ultramicrotomy. You see I
need to fuse two PP wires and image the interface. Now the problem is, when
I'm cutting it with the microtome, the interface just breaks off!!Right now
I'm using a epoxy-hardner ratio of 10:4. will changing the proportions help?
I'm thinking of using a diamond knife also. Does anyone have any bright
ideas?!!Help me out on this!!
Praveena



I am not sure I understand the "fusing" part of your question. However PP
has a Tg of -19 so you need cryo-microtome, if you are not perhaps that is
part of the problem. By the way, hopefully these "panic" stories will not
go on much longer. They are not usual to the list. Steve
Stephen McCartney
Research Associate
Virginia Tech
Materials Institute
2108 Hahn Hall
Blacksburg, VA 24061-0344
USA

TEL: 540-231-9765
FAX: 540-231-8517



From daemon Tue Apr 04 15:00:54 2000



From: Ken Converse :      qualityimages-at-netrax.net
Date: Tue, 04 Apr 2000 09:07:19 -0700
Subject: Horrors in the field

Contents Retrieved from Microscopy Listserver Archives
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To All,
As a field engineer, I fully agree with both Mel and Ron and have always
told my customers, "The only stupid question is the one that you don't
ask."

I will also say that occassionally it can be very difficult to hold
one's temper when someone has done something stupid on an instrument
covered by one's own service contract and then they try to lie about
it. Users, don't add insult to injury. Tell us what you did so that we
can more rapidly repair the damage by looking in the right direction.

Field engineers have horror stories, to:

I became an expert on the ETEC Autospec WDS as an ETEC field engineer by
being impatient in looking for vacuum leaks. The Autospec about doubles
the volume of the system and therefore takes a lot longer to vent. I
had put a 13-1/2 stopper in the port for the secondary detector to see
if the SED was the source of the leak. When it was about half vented,
I pulled the stopper. Without the WDS, this wouldn't have caused any
problem, but I drove the columnator/electron trap into the 4 crystal
turret, broke the tape drive and blew the thin window detector, along
with destroying 2 crystals and loosening all 4. The process of fixing
all this was a three week intensive course in WDS alignment and
operation.

The moral: Don't rush. Take it easy and (God forbid) THINK before you
act. It was only milliseconds to create 3 weeks of work and some $4k in
damaged parts.

Moral #2: Learn from the mistakes of others, because you'll never live
long enough to make them all yourself.

Ken Converse
owner
Quality Images
Delta, PA 17314
717-456-5491



From daemon Tue Apr 04 15:00:56 2000



From: Ken Converse :      qualityimages-at-netrax.net
Date: Tue, 04 Apr 2000 09:14:57 -0700
Subject: MAC drawings

Contents Retrieved from Microscopy Listserver Archives
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Are there any MACs out there?,

I have the original drawings for MAC instruments that ETEC dumped. I
can't vouch for completeness, but there are 4 boxes of tubes with B size
drawings and larger and at least a ream of A size drawings.

I need room. If you need these drawings, please contact me before May
1. After that date I will dump them. They may be had for the cost of
shipping.


Ken Converse
owner
Quality Images
Delta, PA 17314
717-456-5491



From daemon Tue Apr 04 15:00:57 2000



From: Ken Converse :      qualityimages-at-netrax.net
Date: Tue, 04 Apr 2000 09:19:34 -0700
Subject: ETEC Omniscan parts

Contents Retrieved from Microscopy Listserver Archives
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Omniscan users,

Are there any of you left out there? I am trying to decide what to do
with all of the parts aquired from ETEC. If you are still using your
Omniscan, please let me know as I don't want to leave people stranded,
but I could really use the space if there is no need for these parts.


Ken Converse
owner
Quality Images
Delta, PA 17314
717-456-5491



From daemon Tue Apr 04 15:01:04 2000



From: Ken Converse :      qualityimages-at-netrax.net
Date: Tue, 04 Apr 2000 10:30:53 -0700
Subject: Re: Microscope nightmares

Contents Retrieved from Microscopy Listserver Archives
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O.K. I guess I didn't word that as well as I should have :-) However, my
point remains valid - not all the postings are of interest to everyone, no
matter what the content, and these have been fun.

Jan Markham
Motorola FPDD
7700 South River Parkway, FPD22
Tempe, AZ 85284
Ph: (480)755-5509
FAX: (480)755-5115
Email: afp042-at-email.mot.com


-----Original Message-----
} From: John Runions [mailto:cjr41-at-cam.ac.uk]
Sent: Tuesday, April 04, 2000 1:40 AM
To: Markham Jan-AFP042
Cc: 'Kriho, Virginia'; 'List Server'


Garber, Charles A. wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} -- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --
}
} Here is a "just off the press" example (but this does come up at least once
} a year):
}
} I fielded a phone call from a distraught SEM lab manager who told me that
} some months ago he put Dow Corning fluid into his diffusion pump "to save
} money". And now that he has had an accident, the silicone fluid of course,
} has contaminated his system, so he was asking us, "what organic solvent will
} easily remove it."
}
} He was especially upset when I told him that his EDS detector will see Si
} everywhere also, because they do a lot of analyses for Si!
}
} I won't repeat what he told me when I tried to explain the reality of his
} situation......
}
} But it does go to show that there are a lot of new people entering our
} profession, some with less training and experience with vacuum than others,
} so such stories are very well worth repeating.
}
} But just out of curiosity, is there some "recommended procedure" for
} removing silicone fluid from the internal parts of a column, and also
} removing it from the window of an EDS detector? I presume one can always
} call in an outside service provider with experienced people but a lot of
} users out there just don't have the budget for something like that. But
} they do have a good supply of student manpower.
}
} While we are on the subject of silicone, a few weeks ago a well known TEM
} user got me on the phone to say "hello" and commented that he had just
} placed an order for silicone grease and yes, he said, he was going to be
} using it on the o rings of his column. I told him I thought that he should
} be using other greases and his response was "don't listen to dogma, I
} thought you read the listserver!". Am I correct, namely that one should not
} be using silicone grease on the o rings of a column instrument?
}
} Chuck
}
} ============================================
}
} Charles A. Garber, Ph. D. Ph: 1-610-436-5400
} President 1-800-2424-SPI
} SPI SUPPLIES FAX: 1-610-436-5755
} PO BOX 656 e-mail:cgarber-at-2spi.com
} West Chester, PA 19381-0656 USA
} Cust.Service: spi2spi-at-2spi.com
}
} Look for us!
} ########################
} WWW: http://www.spi.cc
} ########################
} ============================================
Chuck,
I agree that he shouldn't be using silicone grease on his o-rings.
Braycote 803 and 602 are what I use, for static and dynamic seals,
respectively.

My experience with DC705 (Transene Vacoil-S), which all ETECs were
shipped with, is that if the system is properly trapped and the vacuum
logic is well thought-out, it seems to work just fine. The only user
that I'm aware of that had problems with stray Si readings was one who
had a SIMS system attached. SIMS is apparently very sensitive to Si.

If you burp your DP, it is diffcult to clean, although ispropanol seems
to work fairly well. I've seen many burped systems, but have never had
any latent problems with silicates causing excessive charging. The
biggest plus of DC705 is that you can take it to atmosphere hot and the
oil is indestructible. It's performance figures are quite acceptable,
and, lets face it, most people don't think twice about what they stick
in their vacuum system. A bullet-proof oil is nice to have.

Perhaps it's more of a problem on systems that don't have a sealed,
full-length liner tube. I'm certainly open to thoughts on why, in 23
years of servicing SEMs, I haven't seen this problem.

Ken Converse
owner
Quality Images
Delta, PA 17314
717-456-5491



From daemon Tue Apr 04 15:01:04 2000



From: Harrison, Gail :      Gail.Harrison-at-reichhold.com
Date: Tue, 4 Apr 2000 11:48:35 -0400
Subject: Hot Stage Retrofit

Contents Retrieved from Microscopy Listserver Archives
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Good Morning All

A colleague has recently requested analysis with a hot stage light
microscope. As I do not have one, I am looking to retrofit a very old
Reichert compound microscope with a hot stage. Is there anyone out there
who might be able to help me?

Thank you

Gail Harrison
Reichhold
919.990.8285



From daemon Tue Apr 04 15:01:07 2000



From: CrushStone-at-aol.com
Date: Tue, 4 Apr 2000 12:25:46 EDT
Subject: Re: Operator Horror Stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At Martin Marietta Labs (1977), Harry O. and myself were responsible for
training, use, and maintenance of an ISI Mini-SEM. One morning we found the
SEM with a blown filament after late-night operation by user or users
unknown. Further investigation revealed the remains of a house fly affixed
with Aquadag to a specimen stub -- and dispersed throughout the microscope.
The innards of the house fly in the microscope prevented the SEM from
reaching operating vacuum. It took two days to adequately clean the column
and the vacuum system.

Apparently the guilty party wanted to examine a housefly in the SEM, but did
not think about it exploding in high vacuum. The fact the fly was not
sputter coated suggested the likely guilty party. He later confessed and was
denied further access without close supervision.

Steve Stokowski
Stone Products Consultants
10 Clark St., Ste. A
Ashland, Mass. 01721
508-881-6364 (ph. and fax)
http://members.aol.com/crushstone/petro.htm



From daemon Tue Apr 04 15:01:15 2000



From: rlvaughn-at-unmc.edu
Date: Tue, 4 Apr 2000 12:44:43 -0500
Subject: Re: re no more horror

Contents Retrieved from Microscopy Listserver Archives
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Oh oh
here comes the fight between the "bios" and the "mats". Jan's comment
didn't bother me, I'm a bio, but to each their own. I think most materials
stuff is also uninteresting. (neat pictures but...)

Rick Vaughn



From daemon Tue Apr 04 15:01:15 2000



From: Anthony Garratt-Reed :      tonygr-at-mit.edu
Date: Tue, 04 Apr 2000 13:30:42 -0400
Subject: Re: Microscope Horror Stories

Contents Retrieved from Microscopy Listserver Archives
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Friends and colleagues

I realize this thread is getting tired, but as this one happened to me just
yesterday, perhaps you'll bear with me as I tell it.

For those of you that don't know the instrument, the XL30 ESEM, like most
modern SEM's, displays its image as 256 intensity levels on a computer
monitor. Presumably the colour could be set to whatever the user chooses,
but we use the default black-and-white.

I had a young photorapher working for a prestigious magazine who needed an
SEM photograph of a human hair. I had the microscope set up before he
arrived, so there was an image on the screen as he walked in. After a few
minutes, he asked "I thought you said this was one of your own hairs".
"Yes", I replied. He looked puzzled, looking closely at my head, then said
"Did you take one of the grey ones?"

Tony.



** Anthony J. Garratt-Reed
** MIT Room # 13-1027
** 77 Massachusetts Avenue
** Cambridge, MA 02139-4307
** USA
**
** Phone: (+) 1-617-253-4622
** Fax: (+) 1-617-258-6479
**




From daemon Tue Apr 04 15:01:20 2000



From: rlvaughn-at-unmc.edu
Date: Tue, 4 Apr 2000 12:31:43 -0500
Subject: re no more stories

Contents Retrieved from Microscopy Listserver Archives
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clogged? I have a whole 13 since yesterday among another dozen of real
junk.

I can't believe some of these things have happened! I have been trying to
dismiss all the anti user ideas that the faculty had here. Don't let
students use it, don't let researchers use it, don't teach them how -- they
can't remember how to use it. I was once a student and learned how to tear
down an old ISI SEM to clean after a filament change (with manual valving).
So if taught and monitored I said, then they should enjoy the fun part of
EM. It's worked so far. (cross my fingers and knock on wood)

I don't have any stories but heard at a meeting of a visiting researcher
from the Far East that was pipeting Osmium by mouth. When corrected he
shook his head yes but was found doing it again so they banned him from the
lab.

Rick Vaughn



From daemon Tue Apr 04 15:01:24 2000



From: Barbara Foster :      mme-at-map.com
Date: Tue, 04 Apr 2000 15:29:11 -0700
Subject: Re: Hot Stage Retrofit

Contents Retrieved from Microscopy Listserver Archives
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Gail,

Most stages have the holes necessary to accept any of the conventional hot
stages.

By the way, do you know which model of Reichert do you have?

Best regards,
Barbara Foster
Consortium President
Microscopy/Microscopy Education ...Educating microscopists for greater
productivity.

125 Paridon Street Suite 102 Springfield, MA 01118
PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com
Visit our web site {http://www.MME-Microscopy.com/education}
******************************************************


At 11:48 AM 4/4/00 -0400, Harrison, Gail wrote:
} ------------------------------------------------------------------------
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From daemon Tue Apr 04 15:01:25 2000



From: Michael Bode :      mb-at-Soft-Imaging.com
Date: Tue, 4 Apr 2000 13:35:27 -0600
Subject: Re: extended focus software

Contents Retrieved from Microscopy Listserver Archives
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Just for clarification:

our software, the EFI module for analySIS, DOES take into account the
lateral shift typical for the dissecting microscopes. It corrects for
this shift first before attempting to reconstruct the final image.

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Rosemary White [mailto:Rosemary.White-at-pi.csiro.au]
Sent: Tuesday, April 04, 2000 3:45 AM
To: microscopy-at-sparc5.microscopy.com


Dear Dave,

There are a couple of options. Autoquant will do this, as will modules
from Soft Imaging Systems. I think some Zeiss software can do this too,
as
long as there is no lateral shift in sequential images (as is found in
some
dissecting scopes). These are packages we've tried, there may well be
more.

cheers,
Rsoemary


Rosemary White
Microscopy Centre
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia

phone 61-2-6246 5475
fax 61-2-6246 5000
email r.white-at-pi.csiro.au




From daemon Tue Apr 04 15:53:04 2000



From: Chuck Butterick :      cbutte-at-ameripol.com
Date: Tue, 04 Apr 2000 15:31:12 -0600
Subject: Service Horrors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


My horror story is about 3 very competent service engineers, all from
the same company, who each blew the window in the same EDX detector in
their own turn. Engineer #1 was just unlucky, I think. He was
installing a STEM unit and the window just popped. Engineer #2 had
disassembled the TEM goniometer and for some unknown reason turned on
the roughing pumps. Evidently the inrush of air increased pressure on
the window enough to cause it to pop. Engineer #3 just didn't listen
to me. He had insisted that my detector bellows was causing a very
small leak. He had taken the detector off 3 times, sure he would
finally demonstrate that without the detector the TEM would hold
vacuum. Each time, though, the vacuum would drift and he'd go find
and solve another leak. After the 3rd time, I told him to not mess
with the detector anymore for fear he would break it. Two days later
after I returned from giving an out-of-town lecture, the detector was
off the scope and the window was indeed blown...and the vacuum leak
was still not solved. I ended up paying for a percentage of the last
repair because the engineer had gotten permission to remove the
detector from my trainee technician (2 months experience). The
bellows was proved to be tight and a new butterfly valve solved the
vacuum leak.

The companies and engineers remain nameless.

Chuck Butterick
Engineered Carbons, Inc.
Borger, TX



From daemon Wed Apr 05 07:54:43 2000



From: Markham Jan-AFP042 :      AFP042-at-email.mot.com
Date: Tue, 4 Apr 2000 15:46:46 -0700
Subject: Re: re no more horror

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Oh no..... I really didn't mean to start another bio vs materials war. I
just meant to point out that we have a variety of interests represented here
and not every item is going to be relevant to every reader at every time (as
a pharmacist, I have done some pharmacognosy in the past and enjoyed it). If
you don't like something, just use the delete button and let others make
their own judgements.

Pax everyone!

Jan Markham
Motorola FPDD
7700 South River Parkway, FPD22
Tempe, AZ 85284
Ph: (480)755-5509
FAX: (480)755-5115
Email: afp042-at-email.mot.com


-----Original Message-----
} From: "rlvaughn-at-unmc.edu"-at-sparc5.microscopy.com
[mailto:"rlvaughn-at-unmc.edu"-at-sparc5.microscopy.com]
Sent: Tuesday, April 04, 2000 10:45 AM
To: Microscopy-at-sparc5.microscopy.com


Oh oh
here comes the fight between the "bios" and the "mats". Jan's comment
didn't bother me, I'm a bio, but to each their own. I think most materials
stuff is also uninteresting. (neat pictures but...)

Rick Vaughn



From daemon Wed Apr 05 07:54:43 2000



From: Markham Jan-AFP042 :      AFP042-at-email.mot.com
Date: Tue, 4 Apr 2000 15:48:08 -0700
Subject: Re: re no more horror

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Oh no..... I really didn't mean to start another bio vs materials war. I
just meant to point out that we have a variety of interests represented here
and not every item is going to be relevant to every reader at every time (as
a pharmacist, I have done some pharmacognosy in the past and enjoyed it). If
you don't like something, just use the delete button and let others make
their own judgements.

Pax everyone!


Jan Markham
Motorola FPDD
7700 South River Parkway, FPD22
Tempe, AZ 85284
Ph: (480)755-5509
FAX: (480)755-5115
Email: afp042-at-email.mot.com


-----Original Message-----
} From: "rlvaughn-at-unmc.edu"-at-sparc5.microscopy.com
[mailto:"rlvaughn-at-unmc.edu"-at-sparc5.microscopy.com]
Sent: Tuesday, April 04, 2000 10:45 AM
To: Microscopy-at-sparc5.microscopy.com


Oh oh
here comes the fight between the "bios" and the "mats". Jan's comment
didn't bother me, I'm a bio, but to each their own. I think most materials
stuff is also uninteresting. (neat pictures but...)

Rick Vaughn



From daemon Wed Apr 05 07:54:44 2000



From: Markham Jan-AFP042 :      AFP042-at-email.mot.com
Date: Tue, 4 Apr 2000 16:31:14 -0700
Subject: Re: re no more horror

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Oh no..... I really didn't mean to start another bio vs materials war. I
just meant to point out that we have a variety of interests represented here
and not every item is going to be relevant to every reader at every time (as
a pharmacist, I have done some pharmacognosy in the past and enjoyed it). If
you don't like something, just use the delete button and let others make
their own judgements :-)

Pax everyone!

Jan Markham
Motorola FPDD
7700 South River Parkway, FPD22
Tempe, AZ 85284
Ph: (480)755-5509
FAX: (480)755-5115
Email: afp042-at-email.mot.com


-----Original Message-----
} From: "rlvaughn-at-unmc.edu"-at-sparc5.microscopy.com
[mailto:"rlvaughn-at-unmc.edu"-at-sparc5.microscopy.com]
Sent: Tuesday, April 04, 2000 10:45 AM
To: Microscopy-at-sparc5.microscopy.com


Oh oh
here comes the fight between the "bios" and the "mats". Jan's comment
didn't bother me, I'm a bio, but to each their own. I think most materials
stuff is also uninteresting. (neat pictures but...)

Rick Vaughn



From daemon Wed Apr 05 07:54:45 2000



From: EBMet-at-aol.com
Date: Tue, 4 Apr 2000 19:48:49 EDT
Subject: Confirmation of New Subscription

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


This is to confirm my subscription to the listserver and to ask my first
question.

I wish to purchase a PC based scanner with a transparency adaptor to scan in
TEM negative plates for digital processing and output. I'd like to keep the
cost of the hardware under $1000. What hardware is available out there? I
have looked at specs for HP 6390C and UMAX Powerlook III. What minimum specs
should I be looking for? Thanks.

Elliot Brown



From daemon Wed Apr 05 07:54:47 2000



From: Marti, Jordi :      jordi.marti-at-honeywell.com
Date: Tue, 4 Apr 2000 13:59:22 -0700
Subject: RE: ultramicrotomy: PP wires

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Praveena:

You don't say what the diameter of the wires is. If possible, you might try
reducing the size of the sample area that needs to be sectioned. I don't
know if you are sectioning at room temperature or cryo. The Tg of PP is
around -19C, so if you are not doing cryo you might want to give that a try.
A diamond knife should work better. Also, depending on what it is you are
trying to see, if you can live with slightly thicker sections then I would
try that.

Hope this helps!

Jordi Marti
Honeywell.



From daemon Wed Apr 05 07:55:00 2000



From: jim :      jim-at-proscitech.com.au
Date: Wed, 5 Apr 2000 12:08:39 +1000
Subject: CPD windows

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have received a another message concerning CPD window material (in the
earlier string a Polaron/ VG manager noted that their windows were not quartz,
but some unspecified superior material.

Ted Pella has advised that they are now using -

"sapphire windows (2 of them, one under the other), which are in turn covered
by the bullet proof shield. I think sapphire has about 50% greater strength
compared to quartz (about 9,000 psi vs about 6,000) - that's why we use
sapphire, for greater safety. We have never
heard of any accident with our unit.

We added the bullet proof shield ("Lexan") to add a second safety
measure for the viewing ports.

Three other safety measures are included: rupture disc,
over-temperature switch and over-pressure switch."

I have no doubt that all manufacturers of CPD are most concerned and make these
units safe; afterall one accident could cost a year's manufacturing cost.
Its nice to know what technology is now in use, thank you Ted Pella.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com



From daemon Wed Apr 05 07:55:00 2000



From: Frederick Schamber :      fhscham-at-sgi.net
Date: Wed, 05 Apr 2000 00:36:53 -0400
Subject: Re: SEM: gun emission and biasing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


shAF,

Regarding the anomalous variation in beam current with emission which you
observe between your instruments:

This is a good demonstration of the fact that as far as emission is
concerned, more isn't always better. I commonly compare the problem of
getting the best performance from the gun to the idea of trying to squirt
water through a knothole some distance away -- the critical parameter isn't
the flow rate of the hose, but rather the ability to direct it into a
focused stream. In reality, the vast majority of the emission never makes
it into the column (compare the emission current to your maximum beam
current) so the quality of the emission is more important than the
quantity. The dynamic of the triode gun is that as you reduce the bias
(thereby increasing the size of the emitting area of the filament and
generating more emission) you also reduce the amount of "focusing" and thus
the emission is less convergent and a smaller fraction passes through the
anode. Whether there is a beam increase or decrease depends on the specifics
of where the gun is operating. In theory, you should be able to observe
this "reverse trend" ( beam current goes down as emission increases) by
reducing the bias sufficiently far on any instrument -- though a particular
instrument may not have the range of bias adjustment to permit this.

Hope this helps.

Fred Schamber
RJ Lee Instruments Limited

=shAf= wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} I've just noticed behavior which is different for my 2 e-beam
} instruments. If I bias my microprobe's gun for less emission, the
} beam current measured in a specimen faraday cup also goes down.
} However, if I bias my SEM's gun for less emission, the beam current
} (measured similarly) goes up(???). This may mean simply a shallower
} optic angle for the SEM and the anode allowing more electrons to pass,
} but I thought I'd throw the observation out there.
}
} cheerios, shAf
}
} {} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {}
} Michael Shaffer, R.A. - ICQ 210524
} Geological Science's Electron Probe Facility - University of Oregon
} mshaf-at-darkwing.uoregon.edu - http://darkwing.uoregon.edu/~mshaf/



From daemon Wed Apr 05 07:55:04 2000



From: Colin Reid :      creid-at-tcd.ie
Date: Wed, 5 Apr 2000 06:46:50 +0100
Subject: RE: Microscope Horror( Humour ) Stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Reminds me of a recent thing that happened here:-

A former colleague spent approx. six hours ( over three separate days )
analysing metal wear particles on the SEM. He was doing the work with a
French engineer who had prepared the samples. She told which particles to
analyse and he dutifully analysed them. I happened to pass and glanced at
the screen and asked why they were analysing paper fibres. They both
insisted that I was wrong ( unfortunately they didn't take my bet ! ), until
I suggested that they try using the BSE detector. Strangely they had lower
contrast than the Al stub. The engineer is almost finished her PhD now and
my colleague has moved on to become a forensic scientist. Just goes to
show the customer is always right ?


Colin Reid,
Electron Microscope Unit,
Trinity College Dublin,
Dublin 2,
Rep. of Ireland.
Tel: 353-1-6081820
Fax: 353-1-6770438
Email: creid-at-tcd.ie
Home Page: http://www2.tcd.ie/Electron_Microscope/emu/home.htm


-



From daemon Wed Apr 05 07:55:20 2000



From: Jenichen :      Jenichen-at-proscan.de
Date: Wed, 05 Apr 2000 12:28:40 +0200
Subject: Re: TEM-Digital camera recommendations

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


If You are interested please have a look at our High performance slow-scan CCD
for TEM at
http://www.proscan.de/pakete1.htm

Mark YEADON schrieb:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Mike,
}
} You could check out Soft Imaging Systems' MegaView II at:
}
} http://www.soft-imaging-web.de/ {http://www.soft-imaging-web.de/}
}
} I'd like to hear other recommendations too...
}
} Mark
}
} %%%%%%%%%%%%%%%%%%
} Mark Yeadon
} Senior Research Fellow
} Institute of Materials Research and Engineering
} 3 Research Link
} Singapore 117602
}
} Assistant Professor
} Department of Materials Science
} National University of Singapore
} Singapore 119260
}
} TEL: (+65) 874 8591
} FAX: (+65) 872 0785
} Email: m-yeadon-at-imre.org.sg {mailto:m-yeadon-at-imre.org.sg}
}
} -----Original Message-----
} From: Michael Coviello [SMTP:coviello-at-mae.uta.edu]
} Sent: Thursday, March 23, 2000 4:44 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: TEM-Digital camera recommendations
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
}
} -----------------------------------------------------------------------.
}
} Hi Ya'll:
} We are looking for a CCD camera for our Philips 430 TEM. We would
} like
} to get the best camera for the best price, e.g., either buying a
} used
} camera or a new non-Gatan camera (Gatan seems to be twice as
} expensive
} as the others). We would be using the camera for bright field and
} high
} resolution TEM of materials (semiconductors) rather than for
} biological
} specimens. Does anyone have a camera they would like to sell/donate
} or
} does anyone have recommendations as to a less-expensive camera that
} they
} know can be used for materials applications.
} Thanks, Mike Coviello
} Lab Manager
} University of Texas -at- Arlington
}

--
Best regards / Mit freundlichen Gruessen
Dr. Frank Jenichen
Proscan elektronische Systeme GmbH
Tel.: +49 8195 999 -511 Fax: -512
mailto:Jenichen-at-proscan.de
------------------------------------------------------------
More information concerning our products
and services can be found on our website
http://www.proscan.de




From daemon Wed Apr 05 08:13:44 2000



From: Robert.Carlton-at-aventis.com
Date: Wed, 5 Apr 2000 07:57:05 -0500
Subject: Polishing SRM 482 Gold-Copper Wires

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


To all,

I am having some difficulties obtaining a good, clean polish on gold-copper
wires (SRM 482). I have embedded the wires in a thermoset resin, backed the
wires with epoxy and polished with successively smaller SiC grit. I have
tried a variety of final polishes including .25 um diamond, vibratory
polishers, etc. I can achieve an excellent metallographic finish with regard
to smooth surfaces, but I cannot remove some small spots from all of the
wires. These are iron red in polarized light and do not have the morphology
of the polishing compounds. A qualitative analysis by EDS does not show any
elements other than copper and gold. Does anyone have experience polishing
these alloys? Is there something I'm missing?

Thanks to all
Robert Carlton
Aventis Pharmaceuticals
robert.carlton-at-rp-rorer.com




From daemon Wed Apr 05 08:25:18 2000



From: Debby Sherman :      sherman-at-btny.purdue.edu
Date: Tuesday, April 4, 2000
Subject: Fwd: gun emission and biasing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


What a great basis for an SEM exam question. I'm going to tuck this one away until needed!
Debby


Debby Sherman, Manager Phone: 765-494-6666
Microscopy Center in Agriculture FAX: 765-494-5896
Dept. of Botany & Plant Pathology E-mail: sherman-at-btny.purdue.edu
Purdue University
1057 Whistler Building
West Lafayette, IN 47907-1057

--------------------------------------


shAF,

Regarding the anomalous variation in beam current with emission which you
observe between your instruments:

This is a good demonstration of the fact that as far as emission is
concerned, more isn't always better. I commonly compare the problem of
getting the best performance from the gun to the idea of trying to squirt
water through a knothole some distance away -- the critical parameter isn't
the flow rate of the hose, but rather the ability to direct it into a
focused stream. In reality, the vast majority of the emission never makes
it into the column (compare the emission current to your maximum beam
current) so the quality of the emission is more important than the
quantity. The dynamic of the triode gun is that as you reduce the bias
(thereby increasing the size of the emitting area of the filament and
generating more emission) you also reduce the amount of "focusing" and thus
the emission is less convergent and a smaller fraction passes through the
anode. Whether there is a beam increase or decrease depends on the specifics
of where the gun is operating. In theory, you should be able to observe
this "reverse trend" ( beam current goes down as emission increases) by
reducing the bias sufficiently far on any instrument -- though a particular
instrument may not have the range of bias adjustment to permit this.

Hope this helps.

Fred Schamber
RJ Lee Instruments Limited

=shAf= wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} I've just noticed behavior which is different for my 2 e-beam
} instruments. If I bias my microprobe's gun for less emission, the
} beam current measured in a specimen faraday cup also goes down.
} However, if I bias my SEM's gun for less emission, the beam current
} (measured similarly) goes up(???). This may mean simply a shallower
} optic angle for the SEM and the anode allowing more electrons to pass,
} but I thought I'd throw the observation out there.
}
} cheerios, shAf
}
} {} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {}
} Michael Shaffer, R.A. - ICQ 210524
} Geological Science's Electron Probe Facility - University of Oregon
} mshaf-at-darkwing.uoregon.edu - http://darkwing.uoregon.edu/~mshaf/






From daemon Wed Apr 05 17:23:29 2000



From: Ed Vicenzi :      vicenzi-at-volcano.si.edu
Date: Wed, 05 Apr 2000 08:51:41 -0400
Subject: SEM Lab Manager Position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Position available at the Smithsonian Institution:

The National Museum of Natural History in Washington, DC is seeking an
experienced electron microscopist to fill a vacancy for SEM laboratory
operation and management. The SEM facility is designed to serve both the
biological and geological research communities in the museum, and houses
two recent model SEMs and one state-of-the-art environmental microscope (to
be installed in mid-2000). The principal responsibilities include training
staff members and visiting scientists in proper use of equipment and theory
of electron generation and detection, maintenance and troubleshooting all
instrumentation (in conjunction with full service contracts), evaluation of
new developments in SEM technology, and supervision of a support staff
member. The successful applicant will also have the opportunity to gain
experience in Focused Ion Beam (FIB) Microscopy and high spatial resolution
secondary ion mass spectrometry HR SIMS.

This position will fill a federal government vacancy and is offered at the
GS11 ($42,724) or GS12 ($51,204) levels, and carries promotion potential to
grade GS 13. U.S. citizenship is required for this federal position. To
obtain information concerning this vacancy call our automated Jobline at
(202) 287-3102 (24 hours, 7 days a week), press 9 and request vacancy
announcement 00MQ-2069. Announcements will be available beginning April
18th and applications must be received by May 16th, 2000. If questions
arise after receiving and reading through the vacancy announcement please
contact: Dr. Edward Vicenzi (Chair, SEM Lab Manager Search Committee) at
vicenzi-at-volcano.si.edu. The Smithsonian Institution is an equal
opportunity employer.


PLEASE NOTE: this position is open from April 18th to May 16th, 2000.






~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Edward P. Vicenzi
Smithsonian Institution
Department of Mineral Sciences
Washington, DC 20560-0119

(202) 357-2594
(202) 357-2476 (fax)
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~



From daemon Wed Apr 05 17:23:31 2000



From: Thomas A. Baginski :      tombg-at-bictom.usuf1.usuhs.mil
Date: Wed, 5 Apr 2000 10:08:26 -0400
Subject: Re: Horror Stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


A couple decades + ago while provding TEM services for the NIH neurology
group, the Neurosurgeons were impatiently awaiting the Biopsy results of
their surgical patient. The frozen section was not conclusive, and so they
needed a TEM result which would confirm their findings. Not being satisfied
with my answer that the results will take a couple days, or one day at the
very earliest, the surgeon sent his first resident up to my lab and he began
rummaging through my supply cabinets so he can prepare the sample quickly
himself. This is before microwave embedding and fixation. He told me that
he was instructed (by my boss) to cut a very thin slice of tissue (use a very
sharp razor), coat it with a lot of glutaraldehyde (straight out of the
bottle) and stick it into the scope (a Joel 100 at that time). He would be
waiting in the OR with the patient until he got the result. He declared
that he did not need any training. ... I was speechless and could not
believe this attempt...I watched the fate with amusement.
I Volutarily moved on to safer grounds thereafter.

Thomas Baginski

Thomas A Baginski, Room G-230
Technical Coordinator for Microscopy
Uniformed Services University of the Health Sciences
4301 Jones Bridge Road
Bethesda, MD 20814-4799

Voice Phone: 301 295 5691
Fax: 301 319 8218
Email: tbaginski-at-usuhs.mil
Alt Email: tombg-at-bictom.usuf1.usuhs.mil
WebSite: { {http://bic.usuf1.usuhs.mil/index.html



From daemon Wed Apr 05 17:23:36 2000



From: Keith Ryan :      KPR-at-wpo.nerc.ac.uk
Date: Wed, 05 Apr 2000 15:35:17 +0100
Subject: folds in semi-thin resin sections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello All

What is the current folklore on avoiding folds/creases in semi-thin
resin sections? I am cutting a worm, approx. 1 mm in diamter, for
light microscopy and photography. It is typical Annelid, i.e.
external cuticle, muscular body wall with inner coelomic space
containing another hollow tubular structure (the gut). It is embedded
in araldite for normal TEM. I have tried drying sections onto glass
slides at 35, 60, 70 and 110 degrees. They all wrinkle prior to
Toluidine Blue staining on hotplate. I have also stained sections by
flotation overnight prior to drying on slides. Help!

Keith Ryan
Marine Biological Association
Plymouth UK



From daemon Wed Apr 05 17:23:37 2000



From: Kenneth JT Livi :      klivi-at-jhu.edu
Date: Wed, 05 Apr 2000 09:49:47 -0400
Subject: Re: Lessons in horror stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear All,
We've been fortunate here that we've not had too many horror stories (at
least funny enough to print here) even though we've had many users of
greatly differing abilities. Of the mild catastrophes we've had, all have
been invaluable for teaching purposes. They teach new users what can go
wrong and how you can become inadvertently famous by not thinking. They've
also taught me how to write instructions to prevent accidents, and how
creative people can be in coming up with new ways to run the microscope. I
routinely tell stories of past users (without revealing identities) to
novices to lighten the long hours of instruction. I've also gotten into the
habit of attaching the price tag (figuratively) to fixing different
components of the scope. There are the famous $18 plastic knobs, } $300
hexrings, and the $50k if you hit your head on the specimen holder while
it's in the scope (actually happened!). This "scared straight" tactic is
always tempered with humor and encouragement so not to paralyze the faint
of heart. And most of all, we don't heap blame on users for errors. We
encourage honesty, lest we get mysterious cases that take much longer to
decipher (such as the case of the missing holder tip).
Ciao for now,
Ken




From daemon Wed Apr 05 17:23:39 2000



From: =shAf= :      mshaf-at-darkwing.uoregon.edu
Date: Wed, 5 Apr 2000 08:57:58 -0700
Subject: RE: SEM: gun emission and biasing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Fred writes ...

}
} Regarding the anomalous variation in beam current with
} emission which you observe between your instruments:
}
} ...
} ... The dynamic of the triode gun is that as you reduce
} the bias (thereby increasing the size of the emitting area of the
} filament and generating more emission) you also reduce the
} amount of "focusing" and thus the emission is less convergent
} and a smaller fraction passes through the anode.
} Whether there is a beam increase or decrease depends
} on the specifics of where the gun is operating.
} In theory, you should be able to observe
} this "reverse trend" ( beam current goes down as emission
} increases) by reducing the bias sufficiently far on any
} instrument -- though a particular instrument may not have
} the range of bias adjustment to permit this.
} ...

Are you implying any "optimization" at the 1st cross-over by
exploring the relationship of emission versus the number of electrons
which get past the anode?? (... as if, the point at which decreasing
the emission also decreases the beam current implies less than
optimized (?) ...). What are your thoughts for this relationship
regarding the "brightest crossover" versus "extended life of the
electron emitter"??

=shAf= :o)



From daemon Wed Apr 05 17:23:43 2000



From: Rick Powell at Nanoprobes :      rpowell-at-nanoprobes.com
Date: Wed, 5 Apr 2000 12:52:35 -0500
Subject: EM/LM: Antibodies to fluorescein

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Hello Microscopists:

I'm interested in localizing fluorescein-tagged gene probes for microscopy
(in situ hybridization, light and EM) and blotting applications. Does
anyone have any recommendations for the best (strongest labeling)
anti-fluorescein antibody, or any experience comparing different clones,
polyclonal vs. monoclonal, or different suppliers?

Thanks in advance,

Rick Powell
Nanoprobes, Incorporated




From daemon Wed Apr 05 17:23:46 2000



From: Steve Chapman :      PROTRAIN-at-CompuServe.COM
Date: Wed, 5 Apr 2000 14:00:15 -0400
Subject: Gun Bias

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Hi

Yes it is very interesting isn't it?

For those TEM operators who have not seen the effect of changing bias on
the electron beam there is a neat experiment.

1. At "gun saturation" bring the condenser to crossover and magnify
this to fill the screen.

2. Change the bias or emission control (not the filament heating)

Either a) The gun de saturates, if so turn the bias control in the
other direction to increase the bias field
Or b) The intensity increases slightly (the subject of current
mail)
Or c) The intensity decreases

If you see (b) the filament is in an position within the cathode that
allows the full optimisation of the emission system; in my experience few
people run under these conditions.

If you see (c) the filament is not in the optimised position within the
cathode.

For SEM operators

1. Set up in the wave form or graph mode

2. Watch the trace as you increase the bias field (emission reduces)

Either a) With the filament position optimised for efficiency the
trace should rise slightly
Or b) With a filament positioned away from this point the trace
will fall.

For those who do not understand the bias or emission control relationships-

A BIAS control will reduce the emission current when turned clockwise,
higher bias.

An EMISSION control will reduce the emission current when turned
anticlockwise, higher bias, or of you like turn up an emission control and
the reduction in the bias field allows an increase in emission current.

They are acting on exactly the same area of the high voltage circuit but
are simply wired in a different way.

Have fun

Steve Chapman
Senior Consultant
Protrain - for professional training in EM world wide
e-mail protrain-at-emcourses.com
web site www.emcourses.com
Tel 44+ 1280 814774 Fax 44+ 1280814007



From daemon Wed Apr 05 17:23:46 2000



From: A. Greene :      ablue-at-io.com
Date: Tuesday, April 04, 2000 2:30 PM
Subject: Re: re no more horror

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Hello folks,
I was traveling and really wanted to zip in three short "horror stories"
before the thread got yanked or burned. [1] Eons ago (Actually maybe 25
years) I was a service engineer with Philips and went to perform routine
maintenance on a very early EM-300. I always cleaned the windows and in
this instance, I found the smaller, left, projection window to be Plexiglas!
No one would tell me how long the ersatz window had been in place but I
really made my point when I demonstrated, with a Geiger Counter, that lots
of x-rays were getting sprayed into the room. They quickly got as new
window. I also had a similar experience when a customer stuck the Aluminum
shipping plate in place of a broken window. They also bought a nice new
leaded glass window. See, the window stories are true.
[2] Some of the older microscopes used Mercury Diffusion Pumps to increase
pumping speed. They were usually operated in tandem with the Oil Diffusion
Pump. In one of the not terribly uncommon disasters where a blast of
Mercury blasts up the column and turns all the beautifully Gold plated brass
pieces into a amalgam covered mess, a service engineer who was particularly
resourceful found out that Iodine readily combines with Mercury. He
sprinkled Iodine crystals all over the contaminated parts of the column.
Not long later, there was an explosion! No one was physically hurt, and I
heard the microscope company cleaned up the mess. [3] Our laboratory was
below the level of a creek so when there were sump pump failures, water
would seep onto the floor, fairly quickly. A lovely and dedicated graduate
student was working late a night when one of these pump failures occurred
and she ended up with her feet under water while running our old JEOL 35C
Scanner. I found her and said she would have to stop, as I yanked the wall
switch. She was angry with me for interrupting her research until I was
finally able to convince her she was very close to electrocuting herself.

Best regards,

Alex Greene
SCIENTIFIC INSTRUMENTATION SERVICES, INC.
PMB-499, 1807 West Slaughter Lane, Number 200
Austin, Texas 78748-6200 Phone: 512/282-5507 FAX 512/280-0702

QUALITY ELECTRON MICROSCOPE REPAIRS
-----Original Message-----
} From: "rlvaughn-at-unmc.edu"-at-sparc5.microscopy.com
{"rlvaughn-at-unmc.edu"-at-sparc5.microscopy.com}
To: Microscopy-at-sparc5.microscopy.com {Microscopy-at-sparc5.microscopy.com}



From daemon Wed Apr 05 17:23:56 2000



From: Wil Bigelow :      bigelow-at-engin.umich.edu
Date: Wed, 5 Apr 2000 16:20:57 -0400
Subject: RE: Silicone oils & greases

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The problem with using silicone compounds in electron microscopes is that
if they get on parts where they are struck by the electron beam they break
down to produce siliceous compounds which are non-conductive and so collect
a static electric charge and deflect the electron beam in undesirable ways.
These decomposition compounds are also very insoluble, and become difficult
to remove. I recall our RCA EML microscope, which came charged with
silicone DP oil, developed such deposits on the objective and condenser
apertures. Fortunately, these apertures were made of platinum, and so we
could clean them by heating them in a Bunsen burner to convert the deposits
to silicon oxides and then treating them with concentrated hydrofluoric
acid to dissolve these oxides - something that would be frowned on in most
laboratories these days.

Because of the potential for generating this kind of a problem, I can see
no reason whatsoever for using either a silicone grease or a silicone oil
in a modern electron microscope. In fact, when I was managing electron
microscope laboratories I refused to allow any of these materials in
laboratories out of fear that some inexperienced person would use them on
one of the instruments.

The reason for using a vacuum grease on an O-ring (or gasket) is to provide
enough lubrication so that the O-ring will slide enough to fill the O-ring
groove uniformly, without forming bumps or creases that can cause a leak.
The grease should not be required to produce the basic vacuum seal - the
groove should be smooth enough so that it could seal properly without the
grease if the O-ring fitted into position properly. Thus, only enough
grease should be applied to give a barely visible sheen to the O-ring -
gobs and globs are not needed. While the silicone high vacuum grease is
indeed a good lubricant for O-rings, it is no better than the Brayco and
Krytox greases, which are based on polyphenylether compounds, and which do
not introduce the possibility of having insoluble siliceous compounds
formed on critical parts of the electron optical column. The function,
use, and characteristics of vacuum greases are discussed in more detail in
Chapter 10 of the book "Vacuum Methods in Electron Microscopy"

I also would not use a silicone fluid in a diffusion pump on an electron
microscope, nor other equipment used in preparing electron microscopy
specimens, for the same reason described above. Several diffusion pump
fluids are now available that are nearly as stable to thermal and oxidative
degradation as the silicone fluids, and which have vapor pressure
characteristics that are comparably favorable. These include the
Santovac-5 and Excello-54 polyphenyl ether fluids and the Lion-S and
Alcatel-220 eicosyl naphthalene fluids, all of which will produce levels of
vacuum as good as the DC 705 silicone fluid. These fluids are discussed in
more detail in Section 5.4 of Vac. Meth. in EM.

The problem of removing silicone compounds from parts that have become
contaminated with them is a very difficult one, Because these compounds are
usually very viscous, and are not readily soluble. The Dow Corning
Company, which manufactures them, recommends repeated wiping with cloth
pads moistened with toluene, xylene, trichloroethylene, or
perchloroethylene.

I have recently had fair success cleaning diffusion pump fluids off metal
parts by first wiping the parts with dry paper towels to remove the bulk of
the fluids, then spraying the surfaces with Tilex Soap Scum Remover and
scrubbing them with a cloth pad, then rinsing them with hot running water.
By repeating this process several times I have been able to get acceptable
results in several instances. (I remove the water by rinsing with
isopropyl alcohol, and then dry with a gas blaster.) It might be difficult
to adapt this process to cleaning internal parts of an electron microscope,
however. Other cleaning procedures are described on pp. 69 - 74 of Vac.
Meth. in EM.

Wilbur C. Bigelow, Prof. Emeritus
Materials Sci. & Engr., University of Michigan
Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu;
Fx:734-763-4788; Ph:734-662-5237




From daemon Wed Apr 05 17:23:56 2000



From: mcalabrese-at-rsc.rockwell.com
Date: Wed, 5 Apr 2000 14:23:37 -0700
Subject: Re: Polishing SRM 482 Gold-Copper Wires

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Robert- It could be that the small spots are copper oxide as this polarizes to a
reddish color. Could you check for oxygen with low kv EDS?

-Mike-




From daemon Wed Apr 05 17:23:57 2000



From: Michael Nesson :      nessonm-at-ucs.orst.edu
Date: Wed, 05 Apr 2000 14:40:40 -0700
Subject: Re: folds in semi-thin resin sections

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Hi Keith:

One possible work-around is to cut your sections thinner than usual for
light microscopy, down to 0.4 micro-meters, which then are more likely to
dry wrinkle- free onto your slides. These thin sections then require an
extreme stain to provide sufficient contrast. I refer you to two papers by
del Cerro et al. on such a method, utilizing Stevenel's Blue as the stain.
The articles are in Microscopa Acta Vol. 83, pp.117-121 and 217-220 (1980).

Hope this helps,
Mike Nesson

Keith Ryan wrote:

}
} What is the current folklore on avoiding folds/creases in semi-thin
} resin sections? I am cutting a worm, approx. 1 mm in diamter, for
} light microscopy and photography. It is typical Annelid, i.e.
} external cuticle, muscular body wall with inner coelomic space
} containing another hollow tubular structure (the gut). It is embedded
} in araldite for normal TEM. I have tried drying sections onto glass
} slides at 35, 60, 70 and 110 degrees. They all wrinkle prior to
} Toluidine Blue staining on hotplate. I have also stained sections by
} flotation overnight prior to drying on slides. Help!
}
} Keith Ryan
} Marine Biological Association
} Plymouth UK

--
_______________________________________________________________________
Michael Nesson, Ph.D. Department of Biochemistry & Biophysics
2011 Ag&LS, Oregon State University, Corvallis, OR 97331-7305
(541)737-5245 FAX:(541)737-0481 nessonm-at-ucs.orst.edu




From daemon Wed Apr 05 17:23:58 2000



From: Walck, Scott D. :      walck-at-ppg.com
Date: Wed, 5 Apr 2000 17:43:40 -0400
Subject: RE: Horror Stories

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I forgot about this recent one. We had a technician here who I taught how
to develop film for our JEOL 2000FX. It had been awhile since he replaced
the film, so after he left, I wanted to make sure that the film was loaded
into the cassettes with the emulsion side up. When I opened the film
cassette box, I couldn't take out the cassettes. He had put them in 180
degrees around and the slot in the film holders didn't match up with the
alignment bar going up along the side of the box. The sides of the film box
were actually bulging. I had to pry each cassette out of the box with a
screwdriver. I left most of them for him to do the next day. Before
showing him this, I innocently asked him if he had trouble putting the
holders into the box. He said that the last couple were a little hard to
put in. The good thing: he had loaded the cassettes with the emulsion side
up.
-Scott



From daemon Wed Apr 05 17:23:59 2000



From: John Twilley :      jtwilley-at-sprynet.com
Date: Wed, 05 Apr 2000 18:14:36 -0400
Subject: Spare LN2 carboy needed

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Does anyone have a liquid nitrogen dewar in the 20 to 40 liter range
with low evaporative losses that they would like to sell? Please
contact me off-list.

John Twilley
jtwilley-at-sprynet.com



From daemon Wed Apr 05 17:23:59 2000



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Wed, 5 Apr 2000 12:15:12 -1000 (HST)
Subject: RE: Horror Stories

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Here's another oops.

A friend had just finished cleaning his TEM column parts with acetone, as
he had been instructed. Being an impatient young man, he quickly
reassembled everything and, as he was lowering the column back into place,
noticed a few drops of acetone had fallen into the viewing chamber and
onto the phosphorous screen. I guess he had the chamber open for cleaning
as well, because he said he quickly grabbed the canned air and aimed it
into the chamber to blow off the drops. I walked in just then to see a
cloud of yellow dust settling all over the walls and floor of the EM
lab... He cleaned out the viewing chamber as best he could, I suppose,
but there was dust in the column and pumping system for months to
come. Then there were the phosphorescent footprints and fingerprints that
appeared all over the lab for weeks! Now every time I open my viewing
chamber I hold my breath.

Aloha,
Tina

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************



From daemon Wed Apr 05 21:34:39 2000



From: Bernard Kestel :      kestel-at-anl.gov
Date: 05 Apr 00 17:39:02 -0600
Subject: More TEM Scope Hazards:

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------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America



Many years ago, our lab had one "good" electron microscope-a JEOL
200.
Every few years the massive high voltage cable would short out
internally and
require replacement. I watched the service technician unscrew the lock
ring
from the high voltage tank, which was about as large as a refrigerator
to
power many vacuum tubes. He used a chair to get on top of the unit and
pull
the cable out and hand the end to me. I heard a loud snapping sound and
saw
a frightened look on the fellow's face. In a hurry to get the job done,
he
had forgotten to use an insulated grounding rod kept in the area to
bleed
off the charge in the high voltage capacitors. Although he had a small
burn
on his hand, he and I finished the cable swap with a new one.
A few years later, the instrument had a water hose leak which
flooded
all the control cable plugs at the rear of the console. The technician
had
to "rebuild" many of the plugs over a couple of days time. It ran well
for
a year or two and was shut down to make way for a newer instrument. It's
always good to discuss major service procedures with someone to ensure
that
oversights don't occur unnecessarily.
That person later rose to the higher eschelon's of his company.

Bernie Kestel
Material's Science Division
Argonne National Laboratory
9700 So. Cass Avenue
Argonne, IL., 60439



From daemon Wed Apr 05 21:34:40 2000



From: jekman-at-uwm.edu
Date: Wed, 5 Apr 2000 17:47:17 -0500
Subject: RE: Horror Stories

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To: "'Microscopy'" {microscopy-at-sparc5.microscopy.com}


Back during the asbestos craze I was the last "little indian" doing
TEM analysis, the other two left for bigger and better paying jobs
elswhere. My business manangers (who knew the EM was big and
needed electricity, but that was the extent of their knowledge)
suggested cross-training some in-house employees to assist me
with my work. The first guy walked into the scope room chewing
gum (not like a normal human being, more like a horse). I started
showing him the scope (JEOL 100sx) while he sat in the chair
infront of the scope. The phone rang so I turned to answer it.
Meanwhile that cute little handle on the camera door caught the
new guy's attention. "what's this?" I heard, and then that all too
familiar hiss followed by valves closing and pumps and power
switches clicking off. He just reached out and turned the handle,
venting the column. The scope handled the shock better than I did.
I sent the guy to lunch, and locked the door behind him.
Jon Ekman
Associate Research Specialist
University of Wisconsin Milwaukee
414-229-6471



From daemon Wed Apr 05 21:34:41 2000



From: Barbara Foster :      mme-at-map.com
Date: Wed, 05 Apr 2000 18:51:15 -0700
Subject: Need some beautiful stereo pairs

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Hi,

A client of ours has a clever new way to present 3D images but needs some
beautiful stereo pairs to generate a set of tests. These images need to be
true stereo pairs, not anaglyphs. If you have anything you would like to
share, please contact me directly. Also, there is a possibility that these
materials may be used in future publications... of course, with credit, so
please let me know if you would like the images used (a) just for the tests
or (b) for both tests and publication, with your citation.

I look forward to hearing from you all!

Best regards,
Barbara Foster
Consortium President
Microscopy/Microscopy Education ...Educating microscopists for greater
productivity.

125 Paridon Street Suite 102 Springfield, MA 01118
PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com
Visit our web site {http://www.MME-Microscopy.com/education}
******************************************************



From daemon Wed Apr 05 21:34:49 2000



From: Rosemary White :      Rosemary.White-at-pi.csiro.au
Date: Thu, 6 Apr 2000 11:07:13 +1000
Subject: Re: Stretch?? Epoxy sections?

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Dear Hildegard,

To follow up a posting a few days ago, what epoxy are you using that does
not compress during sectioning? I am very curious!

THanks,


Rosemary White
Microscopy Centre
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia

phone 61-2-6246 5475
fax 61-2-6246 5000
email r.white-at-pi.csiro.au




From daemon Thu Apr 06 07:35:35 2000



From: Frederick Schamber :      fhscham-at-sgi.net
Date: Wed, 05 Apr 2000 23:24:47 -0400
Subject: Re: SEM: gun emission and biasing

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{!doctype html public "-//w3c//dtd html 4.0 transitional//en"}
{html}

{blockquote TYPE=CITE}
{pre} =shAf= wrote:
        Are you implying any "optimization" at the 1st cross-over by
exploring the relationship of emission versus the number of electrons
which get past the anode??  (... as if, the point at which decreasing
the emission also decreases the beam current implies less than
optimized (?) ...).  What are your thoughts for this relationship
regarding the "brightest crossover" versus "extended life of the
electron emitter"?? {/pre}
{/blockquote}

{p} {br} Yes, the whole mechanism relies on optimization of the electron
trajectories so that they converge to a dense "crossover" in the region
of the anode opening.  Raw emission increases as bias is reduced but
the amount of focusing is also reduced so that there is an optimum point
where the maximum current is focused into the "virtual aperture" (entrance
pupil) of solid angle where it will end up hitting the specimen.
{p} Understanding the dynamics of the gun is really made much more complicated
by the self-biasing (auto-bias) circuit which nearly every thermionic microscope
uses (field emitters are, of course, a whole different story).  If
one had a direct-bias unit you could freely run the bias from low to high
and clearly see the effects.  At a very low bias, you get a flood
of emission (usually limited only by the rating of the HV power unit) and
at a sufficiently high bias you can cut the emission off entirely. 
However, because almost no one has a direct-bias setup, you can't adjust
the bias directly -- you adjust the bias resistor -- which kind of does
the same thing, but with one big difference -- you are now adjusting the
operating point of the emission-stabilization circuit.  A practical
consequence is that you simply can't achieve cut-off -- since the actual
bias is the voltage drop across the bias resistor due to the flow of emission
current, it is clearly impossible to cut off the emission current completely
(zero current would imply zero bias).  Thus, the bias resistor acts
like it is adjusting the bias directly (except in the opposite sense of
the control) for low resistor values -- but at the other end of the range
it behaves quite differently from a true bias control since it approaches
"cutoff" asymptotically rather than abruptly.
{p} Now look at the other part of the story -- the role of filament temperature. 
If one had a unit with an independent fixed bias, increasing the temperature
would make the emission rise without limit (no "saturation" knee). 
But the autobias circuit means that at some point, as one increases the
filament temperature, the increasing bias (due to the increased voltage
drop across the bias resistor) starts trying to "cut off" the emission
and you reach a stable operating point which microscopists like to call
"saturation" which is, in fact, nothing more than the operating point of
the autobias circuit.
{p} So we have two effects we are trying to match up:  (a) the need
to optimize the projection of the crossover into the column's entrance
pupil; and (b) an autobias circuit which will limit emission once a particular
emitter temperature is reached.  This is the purpose of adjusting
the bias resistor.  By setting it properly, you can make the optimal
convergence condition occur at a desireable "saturation" temperature. 
This condition of optimal convergence is measured by a quantity we call
"brightness", which is the product of the spatial and angular densities
of the beam at the crossover (high brightness means the crossover has a
small diameter and the beam doesn't diverge much -- just what you want
to get the maximum beam down the column).
{p} As to the life of the filament versus maximum brightness:  There
is a well-known equation due to Langmuir which gives the maximum brightness
which can be achieved for a given filament temperature (for a given beam
voltage and material work function).  All other things being equal,
the higher the temperature, the greater the attainable brightness. 
At the same time, the higher the temperature, the greater the rate of filament
evaporation and the shorter its life.  It should be possible on any
microscope to set it up to approach the Langmuir limit over a range of
operating temperatures (assuming appropriate wehnelt spacing and orifice
size).  So you have to make a choice, do you want high brightness
or long filament life?  You can't have both.   It's too
bad that our microscopes don't read filament temperature directly because
then everyone could easily see what the REAL variable regulating filament
life is.  When you adjust the bias resistor, you adjust the emission
stabilization operating point, and since microscopists are taught to "saturate"
the filament, you are thus establishing the filament's operating temperature
and thus its life.
{p} Hope this somewhat long-winded answer addresses your question!
{p} Fred
{br}  
{blockquote TYPE=CITE} Fred writes ...
{p} }
{br} } Regarding the anomalous variation in beam current with
{br} } emission which you observe between your instruments:
{br} }
{br} } ...
{br} } ...  The dynamic of the triode gun is that as you reduce
{br} } the bias (thereby increasing the size of the emitting area of the
{br} } filament and generating more emission) you also reduce the
{br} } amount of "focusing" and thus the emission is less convergent
{br} } and a smaller fraction passes through the anode.
{br} } Whether there is a beam increase or decrease depends
{br} } on the specifics of where the gun is operating.
{br} } In theory, you should be able to observe
{br} } this "reverse trend" ( beam current goes down as emission
{br} } increases) by reducing the bias sufficiently far on any
{br} } instrument -- though a particular instrument may not have
{br} } the range of bias adjustment to permit this.
{br} } ...
{p} =shAf=  :o) {/blockquote}
{/html}


{/html}



From daemon Thu Apr 06 07:35:40 2000



From: Ken Tiekotter :      tiekotte-at-up.edu
Date: Wed, 5 Apr 2000 21:25:56 -0700 (PDT)
Subject: Re: folds in semi-thin resin sections

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Dear Keith,
My experience has been with parasitic helminths. Those of us who work to
any degree of regularity on whole organisms find the varying consistency
of tegument, musculature, and internal organs troublesome. Frequently the
tegument expands differently than the circular, longitudinal, and
tranverse musculature.

I have found the best solution to this situation is to adjust the hardness
of the embedding media to match the most dense material in a given
specimen. In addition to resin hardness, adequate infiltration is a must.

In previous list server responses to wrinkled sections, the techniques of
infiltration and resin hardness, as well as the use of various solvents
and heat to expand sections have been suggested.

Personally, I prefer using LR White methyl methacrylate, medium grade, or
a harder version of the epon replacements with araldite. The difference in
the appearance of the ultrastructure between these two resins will be
obvious, but not necessary objectionable. I find LR White seems to
'relax' more than that of the epoxy resins, is easier to stain with
multiple stains and requires less staining time with u.a. and
pb.cit. However, LR White is the bane of many microscopists and therefore
is not always the resin of choice.

This whole problem is interesting in that so many variables determine
section quality, e.g., how do you pick up the sections, how clean are your
microscope slides, how clean is your boat, how clean are the instruments
you use to transfer sections to the microscope slide, how thick are your
sections, on and on it goes!

My suggestion would be to make certain your slides, your glass knifes and
boats, and the utensils you use to remove the sections are CLEAN! Next,
try waiving a cotton swab dripped in cholorform very close to your
sections and see if your sections expand enough to appear smooth. I
prefer to do this step with the sections already on a droplet on a clean
microscope slide. I watch the sections under the stereoscope to look for
the smooth appearance. In some case, I actually use a heat wand in
addition to the cholorform. If you do not have a heat wand, get a
disposable heat pen (I believe most EM suppliers carry these). You can
either replace the AA batteries when they go dead or, hook the heat wand
to a variable DC transformer. Adjust the voltage to approximately 3 volts
or until the 'filament' is slightly dull red. I usually rub a finger on
my nose, GENTLY touch the filament and then turn on the voltage until the
filament produces a wisp of smoke (strange technique eh?!)

One last comment: I usually turn my hot plate to almost 250 - 300 degree
F. Watch the sections so no bubbles appear and try rocking the slide back
and forth. I would also suggest you very the duration of staining time on
the hot plate. Anything more than a minute and you will surely end up
with some portion of the section lifting from the slide and consequently,
get wrinkles.

Enough rambling... No horror stories with this one!

Cheers!
-Ken
-----------
Ken Tiekotter, Adjunct Professor
The University of Portland
Department of Biology
5000 N. Willamette Blvd.
Portland, OR 97303



On Wed, 5 Apr 2000, Keith Ryan wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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} -----------------------------------------------------------------------.
}
}
} Hello All
}
} What is the current folklore on avoiding folds/creases in semi-thin
} resin sections? I am cutting a worm, approx. 1 mm in diamter, for
} light microscopy and photography. It is typical Annelid, i.e.
} external cuticle, muscular body wall with inner coelomic space
} containing another hollow tubular structure (the gut). It is embedded
} in araldite for normal TEM. I have tried drying sections onto glass
} slides at 35, 60, 70 and 110 degrees. They all wrinkle prior to
} Toluidine Blue staining on hotplate. I have also stained sections by
} flotation overnight prior to drying on slides. Help!
}
} Keith Ryan
} Marine Biological Association
} Plymouth UK
}
}
}



From daemon Thu Apr 06 07:35:48 2000



From: Keith Ryan :      KPR-at-wpo.nerc.ac.uk
Date: Thu, 06 Apr 2000 09:11:34 +0100
Subject: Re. folds in sections

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Hello again

Thanks for the responses - 14 to date.

Most mention using a heat pen or solvent vapour - I don't normally do
this in the belief that the hotplate does the job anyway. It is set
somewhere between 100-120 degrees.

My belief is that the problem is the specimen being a set of
concentric tubes of slightly varying consistency?

Cleanliness has also been mentioned! I am normally perfect - of
course (my wife told me so!).

The most interesting "wrinkle" (I had to get that pun in somewhere)
is to vary the time on the hotplate. I will play with this today. I
have been drying for a few minutes, with sections covered by a dish to
prevent any effect from draughts (I go back a bit - cutting sections
since 1969 - you'd think I have got it right by now!).

I was thinking also of removing the resin to maybe lessen the
wrinkles' effect (sodium methoxide - made with saturated NaOH in
methanol).

Keith

_______________________
Keith Ryan (Dr)
Marine Biological Association
Citadel Hill
Plymouth
Devon PL1 2PB
England

Tel. 0044 (0)1752 633279 (with answer machine)
also 0044 (0)1752 633249
Fax. 0044 (0)1752 633102

e-mail: kpr-at-wpo.nerc.ac.uk



From daemon Thu Apr 06 07:35:53 2000



From: Christiane Macwilliam :      ph95ccm-at-brunel.ac.uk
Date: Thu, 06 Apr 2000 10:51:14 +0100
Subject: iNFORMATION ON VARIOUS TYPES OF MICROPROBE ANALYSIS

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To whom it may concern:
I am a physics undergraduate and I am currently trying to work on an
assignment in Microscopy. I am trying to find material on the theory and
application of electron beam and x-ray methods of analysis with
particular emphasis on SEM, TEM, EPMA, EDX, WDX, AES and XPS. I was
given this website site as a possible source of information. Any
assistance would be greatly appreciated.

Kindest Regards
Chris MacWilliam.
(ph95ccm-at-brunel.ac.uk)



From daemon Thu Apr 06 07:35:54 2000



From: Frederick Schamber :      fhscham-at-sgi.net
Date: Wed, 05 Apr 2000 23:24:47 -0400
Subject: Re: SEM: gun emission and biasing

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{!doctype html public "-//w3c//dtd html 4.0 transitional//en"}
{html}

{blockquote TYPE=CITE}
{pre} =shAf= wrote:
        Are you implying any "optimization" at the 1st cross-over by
exploring the relationship of emission versus the number of electrons
which get past the anode??  (... as if, the point at which decreasing
the emission also decreases the beam current implies less than
optimized (?) ...).  What are your thoughts for this relationship
regarding the "brightest crossover" versus "extended life of the
electron emitter"?? {/pre}
{/blockquote}

{p} {br} Yes, the whole mechanism relies on optimization of the electron
trajectories so that they converge to a dense "crossover" in the region
of the anode opening.  Raw emission increases as bias is reduced but
the amount of focusing is also reduced so that there is an optimum point
where the maximum current is focused into the "virtual aperture" (entrance
pupil) of solid angle where it will end up hitting the specimen.
{p} Understanding the dynamics of the gun is really made much more complicated
by the self-biasing (auto-bias) circuit which nearly every thermionic microscope
uses (field emitters are, of course, a whole different story).  If
one had a direct-bias unit you could freely run the bias from low to high
and clearly see the effects.  At a very low bias, you get a flood
of emission (usually limited only by the rating of the HV power unit) and
at a sufficiently high bias you can cut the emission off entirely. 
However, because almost no one has a direct-bias setup, you can't adjust
the bias directly -- you adjust the bias resistor -- which kind of does
the same thing, but with one big difference -- you are now adjusting the
operating point of the emission-stabilization circuit.  A practical
consequence is that you simply can't achieve cut-off -- since the actual
bias is the voltage drop across the bias resistor due to the flow of emission
current, it is clearly impossible to cut off the emission current completely
(zero current would imply zero bias).  Thus, the bias resistor acts
like it is adjusting the bias directly (except in the opposite sense of
the control) for low resistor values -- but at the other end of the range
it behaves quite differently from a true bias control since it approaches
"cutoff" asymptotically rather than abruptly.
{p} Now look at the other part of the story -- the role of filament temperature. 
If one had a unit with an independent fixed bias, increasing the temperature
would make the emission rise without limit (no "saturation" knee). 
But the autobias circuit means that at some point, as one increases the
filament temperature, the increasing bias (due to the increased voltage
drop across the bias resistor) starts trying to "cut off" the emission
and you reach a stable operating point which microscopists like to call
"saturation" which is, in fact, nothing more than the operating point of
the autobias circuit.
{p} So we have two effects we are trying to match up:  (a) the need
to optimize the projection of the crossover into the column's entrance
pupil; and (b) an autobias circuit which will limit emission once a particular
emitter temperature is reached.  This is the purpose of adjusting
the bias resistor.  By setting it properly, you can make the optimal
convergence condition occur at a desireable "saturation" temperature. 
This condition of optimal convergence is measured by a quantity we call
"brightness", which is the product of the spatial and angular densities
of the beam at the crossover (high brightness means the crossover has a
small diameter and the beam doesn't diverge much -- just what you want
to get the maximum beam down the column).
{p} As to the life of the filament versus maximum brightness:  There
is a well-known equation due to Langmuir which gives the maximum brightness
which can be achieved for a given filament temperature (for a given beam
voltage and material work function).  All other things being equal,
the higher the temperature, the greater the attainable brightness. 
At the same time, the higher the temperature, the greater the rate of filament
evaporation and the shorter its life.  It should be possible on any
microscope to set it up to approach the Langmuir limit over a range of
operating temperatures (assuming appropriate wehnelt spacing and orifice
size).  So you have to make a choice, do you want high brightness
or long filament life?  You can't have both.   It's too
bad that our microscopes don't read filament temperature directly because
then everyone could easily see what the REAL variable regulating filament
life is.  When you adjust the bias resistor, you adjust the emission
stabilization operating point, and since microscopists are taught to "saturate"
the filament, you are thus establishing the filament's operating temperature
and thus its life.
{p} Hope this somewhat long-winded answer addresses your question!
{p} Fred
{br}  
{blockquote TYPE=CITE} Fred writes ...
{p} }
{br} } Regarding the anomalous variation in beam current with
{br} } emission which you observe between your instruments:
{br} }
{br} } ...
{br} } ...  The dynamic of the triode gun is that as you reduce
{br} } the bias (thereby increasing the size of the emitting area of the
{br} } filament and generating more emission) you also reduce the
{br} } amount of "focusing" and thus the emission is less convergent
{br} } and a smaller fraction passes through the anode.
{br} } Whether there is a beam increase or decrease depends
{br} } on the specifics of where the gun is operating.
{br} } In theory, you should be able to observe
{br} } this "reverse trend" ( beam current goes down as emission
{br} } increases) by reducing the bias sufficiently far on any
{br} } instrument -- though a particular instrument may not have
{br} } the range of bias adjustment to permit this.
{br} } ...
{p} =shAf=  :o) {/blockquote}
{/html}



{/html}



From daemon Thu Apr 06 07:35:57 2000



From: Ken Converse :      qualityimages-at-netrax.net
Date: Thu, 06 Apr 2000 05:41:28 -0700
Subject: Re: Silicone oils & greases

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Wil Bigelow wrote:
}
} ------------------------------------------------------------------------
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} -----------------------------------------------------------------------.
}
} The problem with using silicone compounds in electron microscopes is that
} if they get on parts where they are struck by the electron beam they break
} down to produce siliceous compounds which are non-conductive and so collect
} a static electric charge and deflect the electron beam in undesirable ways.
} These decomposition compounds are also very insoluble, and become difficult
} to remove. I recall our RCA EML microscope, which came charged with
} silicone DP oil, developed such deposits on the objective and condenser
} apertures. Fortunately, these apertures were made of platinum, and so we
} could clean them by heating them in a Bunsen burner to convert the deposits
} to silicon oxides and then treating them with concentrated hydrofluoric
} acid to dissolve these oxides - something that would be frowned on in most
} laboratories these days.
}
} Because of the potential for generating this kind of a problem, I can see
} no reason whatsoever for using either a silicone grease or a silicone oil
} in a modern electron microscope. In fact, when I was managing electron
} microscope laboratories I refused to allow any of these materials in
} laboratories out of fear that some inexperienced person would use them on
} one of the instruments.
}
} The reason for using a vacuum grease on an O-ring (or gasket) is to provide
} enough lubrication so that the O-ring will slide enough to fill the O-ring
} groove uniformly, without forming bumps or creases that can cause a leak.
} The grease should not be required to produce the basic vacuum seal - the
} groove should be smooth enough so that it could seal properly without the
} grease if the O-ring fitted into position properly. Thus, only enough
} grease should be applied to give a barely visible sheen to the O-ring -
} gobs and globs are not needed. While the silicone high vacuum grease is
} indeed a good lubricant for O-rings, it is no better than the Brayco and
} Krytox greases, which are based on polyphenylether compounds, and which do
} not introduce the possibility of having insoluble siliceous compounds
} formed on critical parts of the electron optical column. The function,
} use, and characteristics of vacuum greases are discussed in more detail in
} Chapter 10 of the book "Vacuum Methods in Electron Microscopy"
}
} I also would not use a silicone fluid in a diffusion pump on an electron
} microscope, nor other equipment used in preparing electron microscopy
} specimens, for the same reason described above. Several diffusion pump
} fluids are now available that are nearly as stable to thermal and oxidative
} degradation as the silicone fluids, and which have vapor pressure
} characteristics that are comparably favorable. These include the
} Santovac-5 and Excello-54 polyphenyl ether fluids and the Lion-S and
} Alcatel-220 eicosyl naphthalene fluids, all of which will produce levels of
} vacuum as good as the DC 705 silicone fluid. These fluids are discussed in
} more detail in Section 5.4 of Vac. Meth. in EM.
}
} The problem of removing silicone compounds from parts that have become
} contaminated with them is a very difficult one, Because these compounds are
} usually very viscous, and are not readily soluble. The Dow Corning
} Company, which manufactures them, recommends repeated wiping with cloth
} pads moistened with toluene, xylene, trichloroethylene, or
} perchloroethylene.
}
} I have recently had fair success cleaning diffusion pump fluids off metal
} parts by first wiping the parts with dry paper towels to remove the bulk of
} the fluids, then spraying the surfaces with Tilex Soap Scum Remover and
} scrubbing them with a cloth pad, then rinsing them with hot running water.
} By repeating this process several times I have been able to get acceptable
} results in several instances. (I remove the water by rinsing with
} isopropyl alcohol, and then dry with a gas blaster.) It might be difficult
} to adapt this process to cleaning internal parts of an electron microscope,
} however. Other cleaning procedures are described on pp. 69 - 74 of Vac.
} Meth. in EM.
}
} Wilbur C. Bigelow, Prof. Emeritus
} Materials Sci. & Engr., University of Michigan
} Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu;
} Fx:734-763-4788; Ph:734-662-5237


Wil,
I have apparently always had a good handle on the problems of silicones
and e-beams. However, it still begs the question of why I haven't seen
these problems in 23 years of servicing ETECs. I've seen charging from
actual droplets present in a contaminated system, but never after
cleaning, and I KNOW I've never gotten a contaminated system really
stripped of silicones. Perhaps it's because one characteristic of ETECs
that customers have told me about is that they continue to image very
well when incredibly contaminated compared to other systems. Just a
thought.

Ken Converse
owner
Quality Images
Delta, PA 17314
717-456-5491



From daemon Thu Apr 06 07:36:03 2000



From: Giovanni Casotti :      giovanni-at-bio.wcupa.edu
Date: Thu, 6 Apr 2000 08:32:26 -0400
Subject: EM Job Opening - Electron Microscopy Technician

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The Electron Microcopy Analysis Center (EMAC) at West Chester University
in Pennsylvania has an opening for a full-time electron microscopy
technician in a multiple user facility. The successful applicant will
have a minimum of a BachelorÕs degree and 3 years experience working with
electron microscopes. Responsibilities will include daily operation and
maintenance of the EMAC including training users on the instrumentation,
specimen preparation and darkroom techniques. The successful applicant
must demonstrate the necessary organizational, management and
communication skills to efficiently operate the EMAC. Applicants must
submit a cover letter describing their experience with different
instrumentation, a resume and contact information for three references.
Although not a requirement, a sample of electron micrographs showing the
applicant's work would be useful for the selection committee. All
materials are to be sent to the Department of Human Resources, 210 Carter
Drive, West Chester University, West Chester, PA, 19383. Applications
must be received by June 1 2000. Applicants must successfully complete
the interview process to be considered a finalist. AA/EOE. Women and
minorities are encouraged to apply.



From daemon Thu Apr 06 07:36:03 2000



From: gcasotti :      gcasotti-at-wcupa.edu
Date: Thu, 6 Apr 2000 08:38:34 -0400
Subject: EM Job Opening - Electron Microscopy Technician

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The Electron Microcopy Analysis Center (EMAC) at West Chester University
in Pennsylvania has an opening for a full-time electron microscopy
technician in a multiple user facility. The successful applicant will
have a minimum of a BachelorÕs degree and 3 years experience working with
electron microscopes. Responsibilities will include daily operation and
maintenance of the EMAC including training users on the instrumentation,
specimen preparation and darkroom techniques. The successful applicant
must demonstrate the necessary organizational, management and
communication skills to efficiently operate the EMAC. Applicants must
submit a cover letter describing their experience with different
instrumentation, a resume and contact information for three references.
Although not a requirement, a sample of electron micrographs showing the
applicant's work would be useful for the selection committee. All
materials are to be sent to the Department of Human Resources, 210 Carter
Drive, West Chester University, West Chester, PA, 19383. Applications
must be received by June 1 2000. Applicants must successfully complete
the interview process to be considered a finalist. AA/EOE. Women and
minorities are encouraged to apply.



From daemon Thu Apr 06 07:52:55 2000



From: White, Woody N :      Woody.N.White-at-mcdermott.com
Date: Thu, 6 Apr 2000 08:27:00 -0500
Subject: Re:Need some beautiful stereo pairs

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Barbara,

I have a number of stereo pairs on my web site, some better than others...

http://woody.white.home.att.net

They are not intended for commercial use for profit, but may be used for
test
purposes. ...They were collected on my own time, but using the company's
equipment.

Woody White
McDermott Technology Inc.
-----------------------------------------------
Hi,

A client of ours has a clever new way to present 3D images but needs some
beautiful stereo pairs to generate a set of tests. These images need to
Best regards,
Barbara Foster
Consortium President
Microscopy/Microscopy Education ...Educating microscopists for greater
productivity.

125 Paridon Street Suite 102 Springfield, MA 01118
PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com
Visit our web site {http://www.MME-Microscopy.com/education}
******************************************************



From daemon Thu Apr 06 07:52:56 2000



From: Purdy, Sam :      SPurdy-at-nationalsteel.com
Date: Thu, 6 Apr 2000 07:40:00 -0500
Subject: RE: Polishing SRM 482 Gold-Copper Wires

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Robert Carleton:
Have ypu considered the possibility that the red spots are particles
of Cu2O? This compound will show up as red partcles in polarized light.

Sam Purdy
National Steel Technical Center
Trenton, MI
V 734-676-2682
F 734-676-2030
spurdy-at-nationalsteel.com

} ----------
} From: "Robert.Carlton-at-aventis.com"-at-sparc5.microscopy.com
} Sent: 5, April 2000, 8:57 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Polishing SRM 482 Gold-Copper Wires
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} To all,
}
} I am having some difficulties obtaining a good, clean polish on
} gold-copper
} wires (SRM 482). I have embedded the wires in a thermoset resin, backed
} the
} wires with epoxy and polished with successively smaller SiC grit. I have
} tried a variety of final polishes including .25 um diamond, vibratory
} polishers, etc. I can achieve an excellent metallographic finish with
} regard
} to smooth surfaces, but I cannot remove some small spots from all of the
} wires. These are iron red in polarized light and do not have the
} morphology
} of the polishing compounds. A qualitative analysis by EDS does not show
} any
} elements other than copper and gold. Does anyone have experience
} polishing
} these alloys? Is there something I'm missing?
}
} Thanks to all
} Robert Carlton
} Aventis Pharmaceuticals
} robert.carlton-at-rp-rorer.com
}
}
}




From daemon Thu Apr 06 17:24:31 2000



From: W. L. Steffens :      bsteffen-at-arches.uga.edu
Date: Thu, 6 Apr 2000 09:24:31 -0400 (Eastern Daylight Time)
Subject: Re: RE: Horror Stories

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On Wed, 5 Apr 2000 17:47:17 -0500
"jekman-at-uwm.edu"-at-sparc5.microscopy.com wrote:

.....
} Meanwhile that cute little handle on the camera door caught the
} new guy's attention. "what's this?" I heard, and then that all too
} familiar hiss followed by valves closing and pumps and power
} switches clicking off. He just reached out and turned the handle,
} venting the column.

I'd be willing to bet that almost everyone with a JEOL TEM can tell a
similar story.
I do wish they would do something about that handle...it sits there
inviting itself to be turned. I've inadvertanly vented the scope
myself several times by hitting it with the back of the chair.

WL Steffens, Ph.D
Dept. of Pathology
University of Georgia



From daemon Thu Apr 06 17:24:31 2000



From: jim :      jim-at-proscitech.com.au
Date: Thu, 6 Apr 2000 23:27:03 +1000
Subject: RE: Horror Stories

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Great thread. I think many an old timers could fill a book with those stories
by themselves.
Why though nothing autobiographical? There is no shame attached if the horror
was a learning experience. We all know that education is expensive.

At only 23 years of age and only 18 months of intermittent "user experience" I
found my self in charge of a small EM Lab with a Phillips 100C as the center
piece. Interesting TEM, with the column in the near horizontal position and a
transmission viewing screen.

There was some minor leak problem with the specimen holder. That TEM design
prepumped around the holder on partial insertion, while a spring loaded ball
sealed that space from the column. The holder was then pushed in completely and
that pushed that ball aside. Simple and effective.

To fix the ball seal I had to open the column and so I also cleaned various
bits and pieces and re-assembled. Pumped and realigned, I then pulled the
specimen holder out, whereupon the TEM inhaled very deeply. I later found that
that spring loaded ball assembly would fit 180 degrees reversed quite well, but
in that position the ball would not roll back to cover the seal when the
specimen holder was withdrawn.

The main damage was a clear center on that lovely transmission viewing screen,
the coating had been vacuumed. It cost $300 then, which is more like $2000
today and I had another opportunity to hone my maintenance skills, cleaning
column, oil and Hg pumps.

It was a very lonely job, with so little experience running that lab without
any other assistance, but I never learned so much as during those 18 months in
that lab.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Thursday, April 06, 2000 8:15 AM, Tina Carvalho [SMTP:tina-at-pbrc.hawaii.edu]
wrote:
}
} Here's another oops.
}
} A friend had just finished cleaning his TEM column parts with acetone, as
} he had been instructed. Being an impatient young man, he quickly
} reassembled everything and, as he was lowering the column back into place,
} noticed a few drops of acetone had fallen into the viewing chamber and
} onto the phosphorous screen. I guess he had the chamber open for cleaning
} as well, because he said he quickly grabbed the canned air and aimed it
} into the chamber to blow off the drops. I walked in just then to see a
} cloud of yellow dust settling all over the walls and floor of the EM
} lab... He cleaned out the viewing chamber as best he could, I suppose,
} but there was dust in the column and pumping system for months to
} come. Then there were the phosphorescent footprints and fingerprints that
} appeared all over the lab for weeks! Now every time I open my viewing
} chamber I hold my breath.
}
} Aloha,
} Tina
}
} ****************************************************************************
} * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
} * Biological Electron Microscope Facility * (808) 956-6251 *
} * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
} ****************************************************************************
}



From daemon Thu Apr 06 17:24:31 2000



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Thu, 06 Apr 2000 09:30:26 -0500
Subject: Yellow dust clouds.....beware

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-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Tina (Weatherby) Carvalho wrote:
=================================================
Here's another oops.

A friend had just finished cleaning his TEM column parts with acetone, as he
had been instructed. Being an impatient young man, he quickly reassembled
everything and, as he was lowering the column back into place, noticed a few
drops of acetone had fallen into the viewing chamber and onto the
phosphorous screen. I guess he had the chamber open for cleaning as well,
because he said he quickly grabbed the canned air and aimed it into the
chamber to blow off the drops. I walked in just then to see a cloud of
yellow dust settling all over the walls and floor of the EM lab... He
cleaned out the viewing chamber as best he could, I suppose, but there was
dust in the column and pumping system for months to come. Then there were
the phosphorescent footprints and fingerprints that appeared all over the
lab for weeks! Now every time I open my viewing chamber I hold my breath.
===============================================================
This might be a lot more serious, healthwise than just another "oops".

Until relatively recently, virtually all TEM viewing screens were coated
with a Cd-containing phosphor. In recent years there has been an attempt to
use non-Cd containing substitute materials (because of their much lower
toxicity levels) but none really seem to be quite as good as the original Cd
containing phosphors. That is why most of the bulk manufacturers of the Cd-
containing phosphors stopped their production (because of liability concerns
) and that is also why you don't see Cd-containing phosphors readily
available for sale any more.

But some of the people who do screen re-coating services, stockpiled some of
the original Cd-containing phosphor because sometimes that is the level of
performance demanded by the customer.

If the phosphor described by Tina was Cd-containing, I really don't think
you want this to be around as an inhalation hazard. I don't know how common
this kind of "oops" has been, but in the event it should happen in your lab,
I would exercise great care to not inhale the particulates and to undertake
a careful clean-up. And while we are on this topic, if you are doing your
own screen coating and using a "lode" of phosphor purchased some years ago,
the chances are very great that it contains Cd, and all precautions should
be taken to avoid inhalation or other exposures when working with the powder



From daemon Thu Apr 06 17:24:33 2000



From: Jorg Wiezorek :      wiezorek+-at-pitt.edu
Date: Thu, 06 Apr 2000 10:01:18 -0400
Subject: TEM - Post-Doc-Position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


University of Pittsburgh
Department of Materials Science and Engineering

Postdoctoral Research Position in Transmission Electron Microscopy


A POSTDOCTORAL POSITION has become available in the area of transmission
electron microscopy of thin film data storage media as part of a
collaborative research program between Professors J.M. Wiezorek and W.A.
Soffa, Department of Materials Science and Engineering of the University of
Pittsburgh, and the Seagate Corporation. Candidates should hold a Ph.D. in
Materials Science, Physics or a related discipline and must have extensive
hands-on experience in a broad range of imaging, diffraction and analytical
electron microscopy characterization techniques, as well as with sample
preparation methods. Demonstrated expertise in the preparation of thin film
samples suitable for high-resolution TEM characterization in plan-view and
cross-section is highly desirable. A basic knowledge of magnetism in
materials and experience in instrument development and/or computer image
processing/simulation would be beneficial. The appointment is for one year
in the first instance and is available after June 01/00. Screening of
applications will begin immediately and will continue until the post is
filled. Applications from under-represented groups, including minorities,
women and people with disabilities are encouraged.

Interested candidates should send a curriculum vitae, including publication
list, and the names of at least three referees with postal addresses,
telephone numbers and E-mail addresses to: Prof. J.M. Wiezorek, Department
of Materials Science and Engineering, University of Pittsburgh, 848 Benedum
Hall, Pittsburgh, PA 15261, USA. Email: wiezorek+-at-pitt.edu


________________________________
Jorg M. Wiezorek, Ph.D.
Assistant Professor
Director - Electron Microscopy
Materials Science & Engineering
University of Pittsburgh tel.: 412-624 0122
848 Benedum Hall fax.: 412-624 8069
Pittsburgh, PA 15261, USA



From daemon Thu Apr 06 17:24:35 2000



From: Michael Bode :      mb-at-Soft-Imaging.com
Date: Thu, 6 Apr 2000 07:57:39 -0600
Subject: Re: TEM-Digital camera recommendations

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Just for clarification:

The MegaView II is a high-resolution side-mounted TEM camera. It
provides large field of view and high frame rates as well as
high-resolution images. For high-resolution TEM images, as in lattice
images, a bottom-mount camera is more suitable. To that end we have the
BioCam. Although the name seems to imply biological applications, the
name is more a "historical" one and does not reflect the current
applications for the camera.

Nestor, if this sounds like an advertisement, I apologize. Out MegaView
II camera was mentioned in connection with high-resolution images in
materials science, for which it may or may not apply, depending on the
actual requirements.

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================

Mark YEADON wrote:

}
------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
} To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
} On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
}
-----------------------------------------------------------------------.
}
} Mike,
}
} You could check out Soft Imaging Systems' MegaView II at:
}
} http://www.soft-imaging-web.de/ {http://www.soft-imaging-web.de/}
}
} I'd like to hear other recommendations too...
}
} Mark
}
} %%%%%%%%%%%%%%%%%%
} Mark Yeadon
} Senior Research Fellow
} Institute of Materials Research and Engineering
} 3 Research Link
} Singapore 117602
}
} Assistant Professor
} Department of Materials Science
} National University of Singapore
} Singapore 119260
}
} TEL: (+65) 874 8591
} FAX: (+65) 872 0785
} Email: m-yeadon-at-imre.org.sg {mailto:m-yeadon-at-imre.org.sg}
}
} -----Original Message-----
} From: Michael Coviello [SMTP:coviello-at-mae.uta.edu]
} Sent: Thursday, March 23, 2000 4:44 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: TEM-Digital camera recommendations
}
}
}
------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
of
} America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
}
}
-----------------------------------------------------------------------.
}
} Hi Ya'll:
} We are looking for a CCD camera for our Philips 430 TEM. We
would
} like
} to get the best camera for the best price, e.g., either
buying a
} used
} camera or a new non-Gatan camera (Gatan seems to be twice as
} expensive
} as the others). We would be using the camera for bright field
and
} high
} resolution TEM of materials (semiconductors) rather than for
} biological
} specimens. Does anyone have a camera they would like to
sell/donate
} or
} does anyone have recommendations as to a less-expensive camera
that
} they
} know can be used for materials applications.
} Thanks, Mike Coviello
} Lab Manager
} University of Texas -at- Arlington
}



From daemon Thu Apr 06 17:24:35 2000



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Thu, 6 Apr 2000 09:19:36 -0500
Subject: TEM: Immunolabeling on old specimens

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

A question for you immuno gurus out there. We have a client who is
interested in looking again at some five-year old blocks, this time in order
to label a protein. The specimens were fixed for standard TEM, i.e., in 2%
glut / 2% paraformaldehyde, followed by osmium. They are embedded in
Epon/Araldite.

I expect that this is going to be a tough one, if it can be done at all. My
question is: are there any secret techniques for optimizing immunogold
labeling in sub-optimal specimens like this? I'm aware of the silver
enhancement methods (although I've never used them). Would that be a good
route to go? Or is it hopeless?

Thanks.

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/





From daemon Thu Apr 06 17:24:36 2000



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Thu, 6 Apr 2000 09:23:03 -0500
Subject: Horror stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hi,

Alright, I've watched this string for days now and resisted the temptation,
but it's just too much. So here's mine, before Nestor shuts the string
down. I know several good ones, but I'll restrict this to one on me.

A year ago (recent history!), I was teaching a lab section in Electron
Microscopy, and although overloaded with students, things were cruising
along reasonably well. One day, however, in a fit of brainlessness, I
instructed a student who was doing cell cultures in how to dehydrate samples
for TEM. We were processing his samples in a culture plate. He was going to
embed in Spurr's, so I took him through the ethanol dehydrations up to 100%,
then moved on to, you guessed it, propylene oxide. The plastic cell culture
plate he was using was not amused. Right in front of both of us, it melted
and "embedded" his samples in a resin I was not prepared to risk a diamond
knife on. His comment was something like, "Is it supposed to do that?"

He finished the semester with flying colors and presented a very respectable
project. Even better, we're still friends.

Randy Tindall

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/





From daemon Thu Apr 06 17:24:40 2000



From: Tom Phillips :      PhillipsT-at-missouri.edu
Date: Thu, 6 Apr 2000 10:23:23 -0500
Subject: rotatable stages for inverted scopes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Anyone have any good sources for a rotatable stages for inverted
scopes? I would like something with good x & y translation but that
allowed the slide or tray on the stage to rotate while keeping
centered. how do other confocal and deconvolution types solve this
problem? thanks for any tips. Tom

Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)



From daemon Thu Apr 06 17:24:42 2000



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Thu, 06 Apr 2000 12:06:45 -0500
Subject: iNFORMATION ON VARIOUS TYPES OF MICROPROBE ANALYSIS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Chris MacWilliam wrote:
=========================================================
To whom it may concern:
I am a physics undergraduate and I am currently trying to work on an
assignment in Microscopy. I am trying to find material on the theory and
application of electron beam and x-ray methods of analysis with particular
emphasis on SEM, TEM, EPMA, EDX, WDX, AES and XPS. I was given this website
site as a possible source of information. Any assistance would be greatly
appreciated.

Kindest Regards
Chris MacWilliam.
(ph95ccm-at-brunel.ac.uk)
=========================================================
I would suggest you visit the MICRO 2000 meeting and exhibition next week at
the Novotel London West Hotel. You can get details from the Royal
Microscopical Society website at www.rms.org.uk .

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.spi.cc
########################
============================================



From daemon Thu Apr 06 17:24:42 2000



From: DESHAYEP-at-ch1.ch.pdx.edu
Date: Thu, 6 Apr 2000 9:05:24 PST
Subject: TEM: Ion Tech ion mill needs stage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have an IonTech (UK manufacture) dual ion thinner which is missing its rotating stage part; this is a
round piece the size of a large washer, with cogs on the perimeter. It is an essential part. Does anyone
know where we can obtain a replacement?

Patrick Deshaye
Geomicrobiology Lab
Portland State University
deshayep-at-ch1.ch.pdx.edu



From daemon Thu Apr 06 17:24:45 2000



From: koyancq3f1nkrz5xza-at-capi.nl
Date: Thu, 06 Apr 2000 12:56:21
Subject: How To Reach Hundreds Of Prospects Every Month

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


_/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/

S P E C I A L R E P O R T -

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On A Shoestring Budget...

_/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/

Let's face it... obtaining leads and reaching prospects is the
life-blood for today's entrepreneur/small business owner.

The big corporations have traditionally had the advantage in this
area. They have the marketing budget that allows them to flood
the marketplace and get hundreds, if not thousands, of leads.

LEADS TURN TO GOLDEN PROSPECTS

Leads turn into prospects and the if the product or service is
appealing, the result can be a substantial PROFIT!

How can you, the entrepreneur or home based small business
person, level the playing field and compete for your share
without taking out a second mortgage on your house?

THE INTERNET IS THE ANSWER

One area the savvy entrepreneur has turned to is the Internet.

But the Internet is huge. How do you stand out? How do you
generate the leads that are so vital for successful sales?

================================================================
Here's a comparison of Internet/Traditional Advertising Methods:

A Direct Mailing to 250,000 prospects through the Postal Service
will cost you about $150,000.

A Banner Ad that reaches 5 million Internet surfers will cost you
in the neighborhood of $100,000.

Bulk E-Mail to 10 Million people can generate hundreds/thousands
of leads for pennies.
================================================================

.Which Advertising Method is the most economical?

.Which Advertising Method almost guarantee's you'll receive
more leads than you could ever wish for?

================================================================

READ WHAT THE EXPERTS SAY

"Many business people are finding out that they can advertise in
ways that they never could have afforded in the past. The cost
of sending mass e-mail is extremely low, and the response rate is
high and quick." - USA Today.

"E-Mail is an incredible lead generation tool." - Crains Magazine

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It's the tested way to reach thousands of people ... turn leads
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Where do I get the e-mail addresses that will help me contact the
millions of people on the Internet?

HERE'S THE ANSWER:

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We've sold mailing lists for over 5 years and we've never
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This is not a rental list that is restricted to a one-time
mailing. You are purchasing an e-mail address list for your own
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DON'T HESITATE or you will miss out on the least expensive, legal
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95/95/NT or a MAC with SoftWindows.

2. "HOW TO GENERATE LEADS WITH BULK E-MAIL" A manual/guide that
addresses the Bulk E-Mail business. Especially useful for
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For fastest service, you may fax your order to: 1-530-504-7707


To order, via credit card simply cut/paste and print out the
EZ ORDER FORM below and fax to our office today.

***** MILLIONS CD - Volume 9 *****

***** NOW ONLY $247! *****

This "Special Price" is in effect for the next 72 hours,
after that we go back to our regular price of $299.00 ...
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We accept Visa, Mastercard, Amex and Checks by Fax.
Fax your order to: 1-530-504-7707

----------------------Cut & Paste----------------------
---------------------EZ Order Form---------------------

_____Yes! I want everything! I am ordering within 72 hours.
Include ALL bonuses along with my 10 Million E-Mail addresses
(don't forget the 972,565 bonus addresses) at the special price
of only $247.00 + shipping as I have indicated below.

_____Oop's I missed the 72 hour "special". I am ordering my 10
million E-Mail addresses at the regular price of $299.00 +
shipping.

***PLEASE SELECT YOUR SHIPPING OPTION***

____I would like to receive my package FedEx OVERNIGHT. I am
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all other International add an *additional* $25 [$40 total] for
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AVAILABLE for shipments to Alaska, Hawaii, Canada or any
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Address List, the addresses are not rented, but are mine to use
for my own mailing, over-and-over. Free bonuses are included,
but cannot be considered part of the financial transaction. I
understand that it is my responsibility to comply with any laws
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********************************************************
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From daemon Thu Apr 06 17:24:46 2000



From: ancq3f1nkrz5xza-at-neva.spb.ru
Date: Thu, 06 Apr 2000 13:00:29
Subject: How To Reach Thousands Of Prospects With E-Marketing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


_/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/

S P E C I A L R E P O R T -

How To Generate Hundreds Of Leads Every Day

On A Shoestring Budget...

_/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/

Let's face it... obtaining leads and reaching prospects is the
life-blood for today's entrepreneur/small business owner.

The big corporations have traditionally had the advantage in this
area. They have the marketing budget that allows them to flood
the marketplace and get hundreds, if not thousands, of leads.

LEADS TURN TO GOLDEN PROSPECTS

Leads turn into prospects and the if the product or service is
appealing, the result can be a substantial PROFIT!

How can you, the entrepreneur or home based small business
person, level the playing field and compete for your share
without taking out a second mortgage on your house?

THE INTERNET IS THE ANSWER

One area the savvy entrepreneur has turned to is the Internet.

But the Internet is huge. How do you stand out? How do you
generate the leads that are so vital for successful sales?

================================================================
Here's a comparison of Internet/Traditional Advertising Methods:

A Direct Mailing to 250,000 prospects through the Postal Service
will cost you about $150,000.

A Banner Ad that reaches 5 million Internet surfers will cost you
in the neighborhood of $100,000.

Bulk E-Mail to 10 Million people can generate hundreds/thousands
of leads for pennies.
================================================================

.Which Advertising Method is the most economical?

.Which Advertising Method almost guarantee's you'll receive
more leads than you could ever wish for?

================================================================

READ WHAT THE EXPERTS SAY

"Many business people are finding out that they can advertise in
ways that they never could have afforded in the past. The cost
of sending mass e-mail is extremely low, and the response rate is
high and quick." - USA Today.

"E-Mail is an incredible lead generation tool." - Crains Magazine

E - M A I L M A R K E T I N G W O R K S ! !

It is the most cost effective, low budget, way to gain leads.

It's the tested way to reach thousands of people ... turn leads
into prospects that result in orders that can build wealth for
the entrepreneurial business person.

The big question is:

Where do I get the e-mail addresses that will help me contact the
millions of people on the Internet?

HERE'S THE ANSWER:

M I L L I O N S V O L U M E 9

***New - 10 Million addresses - Just Released***

The cleanest, most comprehensive e-mail address list in the
world, BAR NONE!

We've sold mailing lists for over 5 years and we've never
compromised on quality. We won't release any address list until
it passes our "high standards" test.

This is not a rental list that is restricted to a one-time
mailing. You are purchasing an e-mail address list for your own
personal mailings and may use it over-and-over.

DON'T HESITATE or you will miss out on the least expensive, legal
and most effective way to market... PERIOD!

_/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/

F R E E B O N U S E S

Order the e-mail address list within 72 hours and we'll include
the following FREE Bonuses...

1. To help you get started we include basic proven Professional
Mailing Software. Not a demo, a full working version (SORRY,
SINCE THE SOFTWARE IS FREE WE CANNOT OFFER TECHNICAL SUPPORT,
however set-up instructions are included). Requirement: Windows
95/95/NT or a MAC with SoftWindows.

2. "HOW TO GENERATE LEADS WITH BULK E-MAIL" A manual/guide that
addresses the Bulk E-Mail business. Especially useful for
beginners. "HOW TO GENERATE LEADS WITH BULK E-MAIL" will answer
most of your questions and concerns about Bulk E-Mail. An
exclusive for our customers... INCLUDED FREE.

***SPECIAL BONUS*** Order within the next 72 hours and receive
an additional 972,565 e-mail addresses as a prompt ordering
bonus. Order Now!

_/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/ _/

O R D E R N O W . . . SAME DAY SERVICE (M-F) if your order is
received before 2pm Pacific. If you have further questions or to
place an order, call toll free: 1-800-375-6456 24hours a day.

For fastest service, you may fax your order to: 1-530-504-7707


To order, via credit card simply cut/paste and print out the
EZ ORDER FORM below and fax to our office today.

***** MILLIONS CD - Volume 9 *****

***** NOW ONLY $247! *****

This "Special Price" is in effect for the next 72 hours,
after that we go back to our regular price of $299.00 ...
Don't delay... you can be in business tomorrow!

We accept Visa, Mastercard, Amex and Checks by Fax.
Fax your order to: 1-530-504-7707

----------------------Cut & Paste----------------------
---------------------EZ Order Form---------------------

_____Yes! I want everything! I am ordering within 72 hours.
Include ALL bonuses along with my 10 Million E-Mail addresses
(don't forget the 972,565 bonus addresses) at the special price
of only $247.00 + shipping as I have indicated below.

_____Oop's I missed the 72 hour "special". I am ordering my 10
million E-Mail addresses at the regular price of $299.00 +
shipping.

***PLEASE SELECT YOUR SHIPPING OPTION***

____I would like to receive my package FedEx OVERNIGHT. I am
including $15 for shipping. (Hawaii & Alaska $20 - Canada $25,
all other International add an *additional* $25 [$40 total] for
shipping)

____I would like to receive my package FedEx 2nd Day delivery.
I'm including $10 for shipping. (Sorry FedEx 2nd Day is NOT
AVAILABLE for shipments to Alaska, Hawaii, Canada or any
International destination - Continental U.S. shipping
addresses only).

***Please Print Carefully***

NOTE: Orders cannot be shipped without complete information
including your signature. No exceptions!


NAME____________________________________________________

COMPANY NAME____________________________________________

ADDRESS_________________________________________________
(FedEx can only ship to street addresses - no P.O. boxes)

CITY, STATE, ZIP________________________________________

PHONE NUMBER____________________________________________
(required for shipping & tracking)


EMAIL ADDRESS___________________________________________
(Print Carefully - required in case we have a question and to
send you a confirmation that your order has been shipped)

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From daemon Thu Apr 06 17:24:48 2000



From: Terence Mitchell :      temitchell-at-lanl.gov
Date: Thu, 6 Apr 2000 11:26:04 -0600
Subject: David S. Phillips, 1952-2000

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


David S. Phillips died suddenly of a heart attack on Saturday March
18, while hiking with his son's Boy Scout Group in Bandelier National
Monument, near Los Alamos, New Mexico.

David was born on February 17, 1952, in Columbus, Ohio. Following
graduation from Fairborn High School, Ohio, in 1970, he attended Case
Western Reserve University in Cleveland, Ohio, where he received his
B.S. degree in Metallurgy with highest honors in 1974. He continued
on at Case for his M.S. and Ph.D. degrees researching in Ceramics
with myself and Arthur Heuer. After a post-doctoral appointment with
Jacques Castaing at the CNRS Laboratoire de Physique des Materiaux in
Bellevue, France, David was on the faculty of the University of
Illinois before joining Los Alamos National Laboratory in 1984.

David is survived by his wife Jane and by their sons Sam and Paul,
all of Los Alamos. A memorial fund has been set up in the name of
David Samuel Phillips at Los Alamos National Bank to benefit his sons.

David Phillips was a fine electron microscopist who applied his
skills to a wide variety of problems in ceramics, superconductors,
diamonds and nuclear materials. Amongst his many accomplishments were
seminal papers on the use of high resolution and analytical electron
microscopy to deduce the underlying cause for the star in star
sapphire. He performed beautiful work in science and in everyday life
and will be sorely missed.




xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx
Terence E. Mitchell
Laboratory Fellow
Materials Science and Technology Division
MS-K765
Los Alamos National Laboratory
Los Alamos, NM 87545
voice mail: 505-667-0938
fax: 505-665-2992
e-mail: temitchell-at-lanl.gov
xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx



From daemon Thu Apr 06 17:24:55 2000



From: DrJohnRuss-at-aol.com
Date: Thu, 6 Apr 2000 14:24:30 EDT
Subject: reminder-image analysis short course

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


There is still some space left for the North Carolina State University short
course on Image Analysis and Stereology, but you should register soon to
avoid disappointment.

There are two sessions of the workshop, May 11-13 and May 15-17, 2000, at N.
C. State Univ. in Raleigh, NC. This course has been heavily attended and
widely praised for the last 17 years. It offers hands-on training using
computer-based image processing and analysis, couple with practical lectures
by experts in the fields of digital imaging and stereology. Many attendees in
the fields of materials, biology, medical applications, food science,
industrial inspection, and other disciplines involving microscopy have found
this course to be a very practical way to gain understanding and proficiency
in the techniques for acquiring and interpreting structural information.

Full information, including course outlines, faculty information, and on-line
registration, is available at
http://members.aol.com/IPCourse/
You may also contact Cindy Allen at 919 515 8171.



From daemon Thu Apr 06 17:24:58 2000



From: David Henriks :      Henriks-at-CompuServe.COM
Date: Thu, 6 Apr 2000 15:59:27 -0400
Subject: TEM: Ion Tech ion mill needs stage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Patrick:

VCR Group, Inc. sold Ion Tech products here in the U.S. many years ago.
When we acquired VCR Group last year, we also acquired some old Ion Tech
parts. I will talk to Vince Carlino and check our stock to see if we have
what you need.

Best regards-

David
Writing at 12:11:36 PM on 04/06/2000

***************************************************************************
************************

David Henriks
Vice President TEL: 800-728-2233
(toll free in the USA)
South Bay Technology, Inc. +1-949-492-2600
1120 Via Callejon FAX: +1-949-492-1499
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

***************************************************************************
************************

} } } } } Please visit us at http://www.southbaytech.com { { { { {

Manufacturers of precision sample preparation equipment and supplies for
metallography, crystallography and electron microscopy.

Message text written by
INTERNET:"DESHAYEP-at-ch1.ch.pdx.edu"-at-sparc5.microscopy.com
} -----------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


We have an IonTech (UK manufacture) dual ion thinner which is missing its
rotating stage part; this is a
round piece the size of a large washer, with cogs on the perimeter. It is
an essential part. Does anyone
know where we can obtain a replacement?

Patrick Deshaye
Geomicrobiology Lab
Portland State University
deshayep-at-ch1.ch.pdx.edu

{



From daemon Thu Apr 06 17:24:58 2000



From: David Bentley :      dlb-at-u.arizona.edu
Date: Thu, 06 Apr 2000 13:08:08 -0700
Subject: Horror

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Haven't seen this one submitted to the list yet, but this is a nightmare
we all have.
Another department on campus was kind enough to give us a TEM, a JEOL
100CXII. It, of course, had minor vacuum leak which put it out of service,
but besides that it was in mint condition. I had planned to move it
myself but was overruled by my boss. There was concern for injury to
myself or others in case it fell. I begged, whined, pleaded, and lost the
discussion. A moving company would transport. I talked to the
administrative person arranging the move and was told that the moving
company said it had all the information it needed. Bells, whistles,
lights, and red flags went off in my mind. I finally got hold of the
moving company supervisor and was told he had done things like this before
and knew what had to be done. I told him several times, NOT to use the
liftgate on his moving truck, to use a forklift. The column was too heavy,
and balanced wrong for liftgates to work well. He assured me HIS truck
could easily lift the 3,000 lbs. More flags waved! I told him one last
time to use a forklift to avoid problems. The day for moving came and this
day, as well as the next two appointments, the movers gave the excuse the
truck clutch was out, more red flags. When finally they did get the clutch
repaired, I unfortunately (fortunately?) was not there to see the
fireworks, they tried to use the lift gate, got the column several inches
off the ground before both hydraulic cylinders for the truck lift gate
sheared off, dropping the gate and giving the column a good bounce. As
good luck would have it, the microscope didn't tip over, just wobbled a
lot. Had it been higher, a lot of damage could have occurred as well as
someone getting hurt. To top this story off, the moving company had the
audacity to try to charge the University for the cost of repairing their
truck. The damage to the microscope was limited to knocking the
intermediate and projector lenses out of alignment and the instrument is
working wonderfully in its new home. "Takes a lickin' and keeps on tickin'."
Later;
David L Bentley
Imaging Facility
The University of Arizona
Tucson, AZ 85721-0036
(520)621-5097



From daemon Thu Apr 06 17:47:26 2000



From: Ed Kurz :      ekurz-at-mail.ims.uconn.edu
Date: Thu, 6 Apr 2000 17:32:18 -0500
Subject: Micro-Raman

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Would this group be a good place to post micro-Raman questions or is there
a better group?

We are just beginning to use this technique. Can anyone recommend a
computer reference library for Raman? How do different wavelength lasers
affect frequency shift and amplitudes?

Thanks,
Ed


Ed Kurz
Institute of Materials Science, U3136
97 North Eagleville Road
University of Connecticut
Storrs, CT 06269-3136
ekurz-at-mail.ims.uconn.edu
(860) 486-4186 phone
(860) 486-4745 fax




From daemon Thu Apr 06 17:55:10 2000



From: vriesg-at-rpi.edu ()
Date: Thu, 6 Apr 2000 17:46:23 -0500
Subject: Re: application of atomic force microscopy

Contents Retrieved from Microscopy Listserver Archives
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Email: vriesg-at-rpi.edu
Name: Gwynne Vriesema
School: Rensselaer Polytechnic Institute

Question: I am doing a project on the application of atomic force
microscopy to making read/write devices that have
a much higher data density than today's magnetic
drives. Do you see any drawbacks to using AFM for
this application? What aspect of AFM is most
important to understand when figuring out how these
drives would work? What are the advantages to using
this method to store data? Thank you for your time!

---------------------------------------------------------------------------




From daemon Thu Apr 06 18:37:07 2000



From: Walck, Scott D. :      walck-at-ppg.com
Date: Thu, 6 Apr 2000 19:21:01 -0400
Subject: "S" -distortion in TEM images JEOL 2000FX

Contents Retrieved from Microscopy Listserver Archives
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I have a JEOL 2000FX and I am getting an "S" distortion in my images at all
mags. The severity differs slightly with mag. I know that the distortion
is there because I am looking at layered structures on Si. I put the
samples with the interfaces parallel to the rod axis. As I move the sample
along the rod axis, the "s" stays in the same place. I looked at the
Kikuchi lines in an on-axis CBED pattern. They do not seem to suffer the
same problem, but there is a little at low camera lengths at the outside
parts of the pattern.

Does anyone know the source of this distortion and is there any cure for it?

-Scott


Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)




From daemon Thu Apr 06 20:15:12 2000



From: James Martin :      James.S.Martin-at-williams.edu
Date: Thu, 06 Apr 2000 21:05:09 -0400 (EDT)
Subject: Re: Micro-Raman

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I've operated a listserv for a couple of years called Irusers-l, which has
been used by scientists who work in museums and laboratories that
use FTIR or micro-FTIR to analyze historic and artistic works. Some
members also use Raman. We've had brief discussions about expanding the
group to any scientist who uses FTIR or Raman, regardless of application.

Would members of this list be interested in joining Irusers-l?

James Martin

Research Scientist in Chemistry
Williams College
james_martin.tripod.com/williams.htm

Director
Orion Analytical, LLC

On Thu, 6 Apr 2000, Ed Kurz wrote:

}
} Would this group be a good place to post micro-Raman questions or is there
} a better group?
}
} We are just beginning to use this technique. Can anyone recommend a
} computer reference library for Raman? How do different wavelength lasers
} affect frequency shift and amplitudes?
}
} Thanks,
} Ed
}
}
} Ed Kurz
} Institute of Materials Science, U3136
} 97 North Eagleville Road
} University of Connecticut
} Storrs, CT 06269-3136
} ekurz-at-mail.ims.uconn.edu
} (860) 486-4186 phone
} (860) 486-4745 fax
}
}
}
}




From daemon Thu Apr 06 20:34:34 2000



From: George Langford, Sc.D. :      amenex-at-amenex.com
Date: Thu, 06 Apr 2000 21:29:13 -0400
Subject: Microscopy horror stories

Contents Retrieved from Microscopy Listserver Archives
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Fellow microscopists -

Waaay back, when I was an instructor at MIT in Cambridge, Mass., I was
helping out in the physical metallurgy laboratory course when we were
teaching the students how to develop glass metallographic plates (told
you it was a long time ago). One of my students, the son of a famous
metallurgist, placed his fully developed plate on the belt of a machine
that he didn't notice was running. We never let him forget the day he
tried to dry his plate in the cylindrical print drier.

George Langford, Sc.D., in PA trying to forget that everyone used
mercury ice-point reference junctions in their thermocouples in the
heat-treating lab in those days ... and how many got tipped over.



From daemon Thu Apr 06 20:54:27 2000



From: Donald Lovett :      lovett-at-tcnj.edu
Date: Thu, 6 Apr 2000 21:51:13 -0400 (EDT)
Subject: seeking recommendation for stereo zoom

Contents Retrieved from Microscopy Listserver Archives
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I have a colleague with advanced presbyopia who is doing consulting work
and needs a stereo zoom to do botanical identification. He wants to stay
below US $1,000.

He has found the following scopes on the web:

TT-5Z and TT-5B at the www.ken-a-vison.com web site

SMZ Bino zoom at www.microscope.org

Any comments on any of these or recommendations for other scopes in his
price range.

Thank you for any good suggestions.

Don

(Direct replies from vendors are welcomed, but please identify your
financial interest in your offers/recommendations.)



______________________________________________________________________
Donald L. Lovett e-mail: lovett-at-tcnj.edu
Assoc. Professor, Dept. of Biology voice: (609) 771-2876
P.O. Box 7718 fax: (609) 637-5118
The College of New Jersey
Ewing, NJ 08628-0718





From daemon Fri Apr 07 07:59:32 2000



From: Diana van Driel :      dianavd-at-eye.usyd.edu.au
Date: Fri, 7 Apr 2000 13:05:12 +1000
Subject: folds in semi-thin resin sections

Contents Retrieved from Microscopy Listserver Archives
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Can you etch the sections with ethoxide (Ehanol + NaOH)? This has
always been my way around the problem for Epon sections.

Diana
--



Diana van Driel
Department of Clinical Ophthalmology
Sydney University
GPO Box 4337
Sydney NSW
AUSTRALIA 2001

Phone 61 2 938 27278/27395
Mob 0412 165 075
Fax 61 2 938 27318



From daemon Fri Apr 07 07:59:47 2000



From: richard.beanland-at-gecm.com
Date: Fri, 07 Apr 2000 09:12:09 +0000 (GMT)
Subject: Re: TEM: Ion Tech ion mill needs stage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Patrick,
Ion Tech changed their name to Atom Tech a few years ago. They can be reached at:

Island Farm Avenue,
West Moseley,
Surrey,
KT8 2UZ
UK

Tel (+44) 181 941 8959
Fax (+44) 181 941 8948
http://www.atomtech.co.uk/

They will supply replacement parts for most of their machines. (While you're about it, get more ceramic resistors, Al cathodes, cathode stops etc.) If you're rejuvenating one of these machines I'd be happy to describe the maintenance and alignment procedures - I have been taking these things to bits and reassembling them for a few years now.

Cheers,


Richard Beanland

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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} -----------------------------------------------------------------------.
}
}
} We have an IonTech (UK manufacture) dual ion thinner which is missing its rotating stage part; this is a
} round piece the size of a large washer, with cogs on the perimeter. It is an essential part. Does anyone
} know where we can obtain a replacement?
}
} Patrick Deshaye
} Geomicrobiology Lab
} Portland State University
} deshayep-at-ch1.ch.pdx.edu
}



From daemon Fri Apr 07 07:59:53 2000



From: John Minter :      minter-at-kodak.com
Date: Fri, 7 Apr 2000 07:23:21 -0400
Subject: Yet another horror story

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Shortly after our laboratory purchased our first analytical electron
microscope, the microscope developed a problem in the electronics.
The microscopist telephoned the vendor's service department who requested
he measure some voltages to help diagnose the problem. The microscopist
dutifully attached clip-leads to the appropriate test points and was
attaching them to the voltmeter when one of the clip-leads slipped off,
fell into the electronics chassis, and shorted out much of the electronics.
The vendor service ingineers were in the lab for weeks repairing the
problem. The rest of the lab teased him about this, giving the the
microscopist the nickname, "clip-lead". A few years later when he
left the group for a promotion to a staff job, he was presented with
a pair of clip-leads where the metallic ends had been coated with
liquid-rubber.

Best Regards,

John Minter
Eastman Kodak Company
Analytical Technology Division
Rochester, NY 14652-3712
Phone: (716) 722-3407
FAX: (716) 477-3029
email: john.minter-at-kodak.com



From daemon Fri Apr 07 07:59:56 2000



From: ard-at-ansto.gov.au (Arthur Day)
Date: Fri, 7 Apr 2000 22:13:22 +1100
Subject: RE: Horror Stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Here's one that involved the fire department:

Early one evening when the only people still in the building were us
students, a guy from along the corridor came into our room saying that he
could smell something downstairs. We all banded together and went down to
investigate. After a while we noticed this sort of thick foggy smoke
beginning to form below the ceiling along the length of the corridor and in
the adjacent large teaching lab. We noticed it wafting down through some
light fittings and gradually getting lower down and thicker. "Has to be
electrical!" "Looks like the whole place could go up any minute!" It
started looking pretty serious. When the fire brigade turned up we were
told to get out as they proceeded to rip out chunks of the ceiling trying
to locate the source. It was a very diffuse source and there was a very
large area of ceiling to rip chunks out of. After a while their
investigation moved upstairs -- where they found our carbon coater happily
chugging away with it's bell jar off and the exhaust line feeding down into
the ceiling cavity! You learn something new every day.

I think we remembered to say thanks....








Arthur Day, Electron Microscope Unit Phone: 61-2-9717-3457
Ansto Materials Division Fax: 61-2-9543-7179
PMB 1, Menai (Sydney), NSW, 2234 Email: ard-at-ansto.gov.au
Australia www: http://www.ansto.gov.au/




From daemon Fri Apr 07 07:59:59 2000



From: Graham.Cheeseman-at-acadiau.ca
Date: Fri, 7 Apr 2000 09:51:23 -0300
Subject: TM SM Surplus Equipment for Donation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


{paraindent} {param} left {/param} {color} {param} 0100,0100,0100 {/param} {FontFamily} {param} Times New Roman {/param} {bigger} The Department of Biology, Acadia University, Wolfville,
NS have two electron microscopes for disposal. The first is
a Zeiss EM9A transmission electron microscope and the
second a JEOL JSN-25S scanning electron microscope.
Both microscopes are operable, but are surplus to the
current needs of the department. The department is willing
to donate these instruments to an institution, providing that
institution is willing to assume the responsibility for removal
and shipping to the new destination. For further information
contact Dr. T.B. Herman (902) 585-1469. {/paraindent}


{nofill}



==============================================================
Graham N. Cheeseman
Biology Dept., Acadia University
Wolfville, Nova Scotia
B0P 1X0
TEL: (902) 585-1316
FAX: (902) 585-1059
INTERNET: Graham.Cheeseman-at-acadiau.ca
==============================================================

{/x-rich}



From daemon Fri Apr 07 17:49:38 2000



From: Tracey M. Pepper :      tpepper-at-iastate.edu
Date: Fri, 07 Apr 2000 08:39:18 -0500
Subject: Cold Horror

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hope this scary one helps some of you!
We are a research and teaching lab (thankfully!) A very lucky (for us)
student was in working on a weekend when she noticed white smoke pouring
out of the lab into the lecture room, grabbed the extinguisher, and put out
the electrical fire that started in the wall due to our specimen rotator
motor shorting out (and not being properly wired). The fire was directly
underneath the 1 gallon storage tanks for 100% ethanol, and pure
Xylene..... {yipe} ! So bad, that it melted but did not break through the
spigots... {big yipe} !
I also learned that when your filling a liquid nitrogen tank, and it gets
full, and it begins to shoot up a little underneath a temperature sensor,
you get to meet the fire chief!
Life is good!





Tracey Pepper
Supervisor
Bessey Microscopy Facility
Iowa State University
ph: 515-294-3872
fax: 515.294.1337



From daemon Fri Apr 07 17:49:39 2000



From: rlvaughn-at-unmc.edu
Date: Fri, 7 Apr 2000 08:49:48 -0500
Subject: Re: TEM: Immunolabeling on old specimens

Contents Retrieved from Microscopy Listserver Archives
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Hi Randy
One good "old" reference is Moise Bendayan and Max Zollinger.
Ultrastructural Localization of Antigenic Sites on Osmicated-fixed Tissues
Applying the Protein A-Gold Technique. J Histochem Cytochem 31 :101-109,
1983
Some times it works some times it doesn't. I would like to see some newer
tricks if they are out there.

Rick Vaughn



From daemon Fri Apr 07 17:49:42 2000



From: Betty Loraamm :      loraamm-at-chuma.cas.usf.edu
Date: Fri, 7 Apr 2000 10:12:46 -0400
Subject: Horror stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


My horror story happened many years ago when I came into work one morning to
find the Philips200 had been left running for a long period of time with no
water coursing through its veins. The column was hot to touch so my
immediate reaction was to turn on the water supply to try to cool it down.
But hoses and o-rings had deteriorated from the heat, so instead of cooling
the instrument down I now had water gushing from everywhere and filling up
the columnâs viewing area so that it looked like a fish bowl. I called my
Philips service engineer and he spent the next 3 days repairing and cleaning
up the scope. He said the stage had come very close to a melt down and if
that had happened I could have just dug a hole and buried the scope in the
back yard. The scope survived to give the lab many years of service, thanks
to Philips engineer, John Braunagel, whose expert service was given without
a grumble or complaint.




From daemon Fri Apr 07 17:49:48 2000



From: Joyce Craig :      j-craig-at-csu.edu
Date: Fri, 07 Apr 2000 10:10:02 -0500
Subject: horror

Contents Retrieved from Microscopy Listserver Archives
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I set up an lab in a hospital with a Siemens microscope-a wonderful
instrument for resolution but a beast to align. The lenses were
physically moved during alignment so that it did look odd to see
different components of the column a few centimeters off. One morning I
came in and a pathologist proudly told me that he had aligned the column
for me. He had straightened it out very nicely and it took a full day
for me to get it back in alignment. It did look nicer the way he did it
but of course it was impossible to use.
Joyce Craig
Chicgo State University



From daemon Fri Apr 07 17:49:48 2000



From: Joyce Craig :      j-craig-at-csu.edu
Date: Fri, 07 Apr 2000 10:12:53 -0500
Subject: sputter coater

Contents Retrieved from Microscopy Listserver Archives
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How long should a gold target last?
Our target looks gold except in the center.
Problem is that we don't seem to get good coating.
Joyce Craig
Chicago State University



From daemon Fri Apr 07 17:49:50 2000



From: Betty Loraamm (BIO) LIF136 :      loraamm-at-chuma.cas.usf.edu
Date: Fri, 7 Apr 2000 11:53:32 -0400 (EDT)
Subject: Horror stories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


My horror story happened many years ago when I came into work one morning
to find the Philips200 had been left running for a long period of time
with no water coursing through its veins. The column was hot to touch so
my immediate reaction was to turn on the water supply to try to cool it
down. But hoses and o-rings had deteriorated from the heat, so instead of
cooling the instrument down I now had water gushing from everywhere and
filling up the column's viewing area so that it looked like a fish
bowl. I called my Philips service engineer and he spent the next 3 days
repairing and cleaning up the scope. He said the stage had come very
close to a melt down and if that had happened I could have just dug a hole
and buried the scope in the back yard. Surprisingly the scope survived to
give the lab many years of service, thanks to Philips service engineer
John B's expert service that he gave without a grumble or complaint.



From daemon Fri Apr 07 17:49:54 2000



From: Wil Bigelow :      bigelow-at-engin.umich.edu
Date: Fri, 7 Apr 2000 12:17:14 -0400
Subject: A funny 'horror' story

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This is a story that was told to me by Dr. Audrey Glauert of Cambridge
University in the U.K. Even though it's not my own, it is so amusing that
I can't help relaying it to you.

It seems that a number of years ago Dr. Glauert spent several months
working in an electron microscopy laboratory in Africa. Every now and then
the water supply to the laboratory would go off making it necessary to shut
the electron microscope down for an extended period of time. Investigation
eventually revealed that the problem arose because the town involved was
getting it's water from a pond that was formed behind a dam that had been
constructed across a nearby river. It seems that this pond was a favorite
site for a herd of hippopotamuses to bathe, and every now and then one of
them would manage to plug up the water inlet to the village water system,
whereupon it was necessary for the villagers to go out and chase the hippos
away and then open the inlet again. Since hippos are not very easy to
chase, this could sometimes cause a rather prolonged period without water
in the electron microscopy laboratory.

Wilbur C. Bigelow, Prof. Emeritus
Materials Sci. & Engr., University of Michigan
Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu;
Fx:734-763-4788; Ph:734-662-5237




From daemon Fri Apr 07 17:49:56 2000



From: W. L. Steffens :      bsteffen-at-arches.uga.edu
Date: Fri, 7 Apr 2000 14:05:53 -0400 (Eastern Daylight Time)
Subject: Re: Microscopy horror stories

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With all these horror stories being shared, its surprising that nobody
has related a "darkroom" tale yet. Surely everyone who has been in
this field for several years has had the unpleasant experience of
walking into the darkroom and finding everything coated with dried
photographic fix residue? My darkroom experiences with users have been
all in all much worse that microscope incidents.

WL Steffens
University of Georgia
Department of Pathology



From daemon Fri Apr 07 17:50:01 2000



From: Graham.Cheeseman-at-acadiau.ca -at-sparc5.microscopy.com
Date: Fri, 7 Apr 2000 09:51:23 -0300
Subject: TM SM Surplus Equipment for Donation

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{paraindent} {param} left {/param} {color} {param} 0100,0100,0100 {/param} {FontFamily} {param} Times New Roman {/param} {bigger} The Department of Biology, Acadia University, Wolfville,
NS have two electron microscopes for disposal. The first is
a Zeiss EM9A transmission electron microscope and the
second a JEOL JSN-25S scanning electron microscope.
Both microscopes are operable, but are surplus to the
current needs of the department. The department is willing
to donate these instruments to an institution, providing that
institution is willing to assume the responsibility for removal
and shipping to the new destination. For further information
contact Dr. T.B. Herman (902) 585-1469. {/paraindent}


{nofill}



==============================================================
Graham N. Cheeseman
Biology Dept., Acadia University
Wolfville, Nova Scotia
B0P 1X0
TEL: (902) 585-1316
FAX: (902) 585-1059
INTERNET: Graham.Cheeseman-at-acadiau.ca
==============================================================


{/x-rich}



From daemon Fri Apr 07 17:50:04 2000



From: Vetrano, John S :      john.vetrano-at-pnl.gov
Date: Fri, 07 Apr 2000 14:00:12 -0700
Subject: Horror: Why my post doc was almost cut short

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About a month after starting my post doc in the lab of Professor Ruhle in
Stuttgart, Germany, I was working on the TEM late one Sunday night. I was using
a double-tilt, analytical, double-specimen holder (read: expensive) and just
putting it away. The holder was gripped in a Gatan holder stand and when I
pushed the protective end sheath onto the tip the stage moved back so the front
stand grip moved onto the narrower part of the holder where it doesn't grip.
The motorized back end was heavy and the holder tipped backwards while I was
still holding the protective end piece firm. What resulted is a holder that
greatly resembled a Concord airplane coming in for a landing. It bent the
holder at about a 30 degree angle right where the hinge was for the back
specimen cup.

I sat and looked at it, beads of sweat forming on my forhead, contemplating the
fact that I had an open ended return ticket and wondering if I could pack my
stuff up and be gone back to the U.S. before anyone noticed. Of course I stayed
and Professor Ruhle was very good about it, basically saying that mistakes
happen but don't do it again! I didn't.

Lessons Learned: Be very careful when handling expensive specimen holders;
think twice before you do anything.

I hope you all have a good weekend and don't mind my confessions!

Cheers, JSV

***************************
John Vetrano
Pacific Northwest National Laboratory
MSIN P8-16
P.O. Box 999
Richland, WA 99352
Phone: (509)372-0724 Fax: (509)376-6308
Email: mailto:john.vetrano-at-pnl.gov



From daemon Fri Apr 07 17:50:08 2000



From: Bernard Kestel :      kestel-at-anl.gov
Date: 07 Apr 00 16:39:01 -0600
Subject: Horror Prevention:

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Maybe Judy Murphy's Delta College will diminish these "untrained"
occurrences!

Bernie Kestel
Argonne National Laboratory



From daemon Fri Apr 07 17:50:08 2000



From: Valerie Leppert :      vjleppert-at-ucdavis.edu
Date: Fri, 7 Apr 2000 14:55:56 -0700 (PDT)
Subject: Mat Sci:Need Tips for Ion Milling Porous Ceramic

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If anyone has any tips or a reference they could point me to for this, it
would be much appreciated.

We have had several users with nanocrystalline ceramic samples from
combustion or plasma syntheses who have had problems with the thin areas
of their samples falling out during ion milling. We have tried a few
schemes to correct this, without much success. Any help would be greatly
appreciated.

The current user has 2 different nanocrystalline composites: TiB2/TiN and
TiB2/TiC.

Valerie Leppert
Dept. Chem. Eng. and Mat. Sci.
University of California, Davis

vjleppert-at-ucdavis.edu



From daemon Fri Apr 07 17:50:09 2000



From: White, Woody N :      Woody.N.White-at-mcdermott.com
Date: Fri, 7 Apr 2000 17:46:00 -0500
Subject: Re:sputter coater

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That is a difficult question since it depends on so many variables (original
target thickness, coating rates, time run, etc). I use my sputter coater
often
(but not as often as the carbon evaporator). Never kept accurate records of
usage, but it is at least several years old.

In any event, the entire target should have a bright gold color. Some areas
may
have a matte vs shiny appearance, but should not be dark. If not all bright
Au,
it is either consumed or contaminated. Either case will cause sputtering to
cease.

Woody White




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How long should a gold target last?
Our target looks gold except in the center.
Problem is that we don't seem to get good coating.
Joyce Craig
Chicago State University



From daemon Fri Apr 07 17:50:09 2000



From: Betty Loraamm :      loraamm-at-chuma.cas.usf.edu
Date: Fri, 7 Apr 2000 10:12:46 -0400
Subject: Horror stories

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My horror story happened many years ago when I came into work one morning to
find the Philips200 had been left running for a long period of time with no
water coursing through its veins. The column was hot to touch so my
immediate reaction was to turn on the water supply to try to cool it down.
But hoses and o-rings had deteriorated from the heat, so instead of cooling
the instrument down I now had water gushing from everywhere and filling up
the columnâs viewing area so that it looked like a fish bowl. I called my
Philips service engineer and he spent the next 3 days repairing and cleaning
up the scope. He said the stage had come very close to a melt down and if
that had happened I could have just dug a hole and buried the scope in the
back yard. The scope survived to give the lab many years of service, thanks
to Philips engineer, John Braunagel, whose expert service was given without
a grumble or complaint.





From daemon Fri Apr 07 17:50:11 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 07 Apr 2000 15:35:17 -0500
Subject: The SCSI interface saga update

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There was some discussion about scanners and SCSI bus
problems. There are some recent developments that I'd
like to share with those who are having SCSI-related problems.

If you have a PC which includes one or two USB ports, there
is a new converter cable which should make your life much
better. Microtech has a USB to SCSI-II converter cable
(# USB-SCSI-HD50) that plugs into a USB socket and provides
a high density 50 pin SCSI-II plug. This plug can be converted
to SCSI-I wide Centronics ribbon-style connector if necessary.
This converter cable works with PCs and Macs; and so far, all
of my SCSI devices work with it (scanners, CD-R, CD-RW).

This item is available from various sources for about $80.
I got mine from d-store.com but you might find other sources.

Hope this helps those having SCSI problems.

gary g.



From daemon Fri Apr 07 17:50:11 2000



From: Cynthia Shannon :      cshannon-at-nctimes.net
Date: Fri, 07 Apr 2000 15:24:09 -0700
Subject: Wanted:Used Zeiss 10c TEM

Contents Retrieved from Microscopy Listserver Archives
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Does anyone have a functioning Zeiss 10c TEM that they would like to
donate to a goverment office for a tax deduction or sell for cheap?
I am the electron microscopist for the county veterinarian and we are
trying to locate a Zeiss 10c to be used for virus identification work on
animals and plants.
Thank you.
Cindy Shannon
cshannon-at-nctimes.net



From daemon Sat Apr 08 08:03:09 2000



From: Barbara Foster :      mme-at-map.com
Date: Fri, 07 Apr 2000 19:47:18 -0700
Subject: Re: application of atomic force microscopy

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Gwynne,

Suggest you visit the sites for both Digital Instruments and
ThermoMicroscopes. Both have application notes which will be helpful.
Also, suggest that you contact Sergei Magonov at DI and Jezz Leckenby at
ThermoM. Both are extremely knowledgeable apps specialists.
Sergei: 805-967-1400
Jezz: (408) 747-1600

Hope this is helpful.

Caveat: MME has no financial interest in either of these systems.
Barbara Foster
Consortium President
Microscopy/Microscopy Education ...Educating microscopists for greater
productivity.

125 Paridon Street Suite 102 Springfield, MA 01118
PH: (413)746-6931 FX: (413)746-9311 email: mme-at-map.com
Visit our web site {http://www.MME-Microscopy.com/education}
******************************************************


At 05:46 PM 4/6/00 -0500, wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Sat Apr 08 08:03:13 2000



From: Terje Dokland :      dokland-at-ima.org.sg
Date: Sat, 8 Apr 2000 10:06:50 +0800
Subject: TEM - Technician position available at IMA, Singapore

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ELECTRON MICROSCOPIST
Laboratory of Structural Biology
Institute of Molecular Agrobiology, Singapore

A position is available for an experienced EM technician at the
Institute of Molecular Agrobiology (IMA), Singapore, in the group of Dr.
Terje Dokland, Laboratory of Structural Biology. The successful candidate
will be responsible for the operation of the structural EM laboratory, and
be involved in several research projects, in particular structural
characterization of various plant and animal viruses of agricultural
importance. Experience with cryo-EM of frozen-hydrated samples is a
definite advantage, as is some experience with using computers, especially
3D reconstruction methods.
IMA is a well funded and rapidly expanding research institute which
was established in 1995 to conduct basic and applied research in
agrobiology. It moved into a modern and spacious building at the National
University of Singapore campus two years ago, and has state-of-the art
equipment for structural and molecular biology, biochemistry, plant growth,
tissue culture etc. There are currently two groups working in structural
biology, including X-ray crystallography and electron microscopy, and there
is extensive collaboration with other laboratories and institutes, both in
Singapore and abroad. Further information about the institute can be found
at http://www.ima.org.sg. Singapore is a cosmopolitan and multicultural
society offering all the conveniences of a modern city, and is centrally
located in Southeast Asia with easy access to other countries in the
region.
The position will be filled at the Research Officer or Assistant
Research Officer level, depending on experience, and the contract will be
initially offered for two years. IMA offers attractive salaries with bonus
packages and benefits.
Informal enquiries are welcome and can be directed to Terje Dokland
at dokland-at-ima.org.sg. Applications, including a CV and names of two
referees, should be sent to
Terje Dokland
Institute of Molecular Agrobiology
1 Research Link
The National University of Singapore
Singapore 117604


------------------------------------
Terje Dokland
Senior Scientist
Institute of Molecular Agrobiology
1 Research Link
The National University of Singapore
Singapore 117604
Phone: 65-872 7405 Fax: 65-872 7007
E-mail: dokland-at-ima.org.sg

"Convictions are greater enemies of truth than lies"
F. Nietzsche




From daemon Sat Apr 08 08:03:18 2000



From: Valerie Leppert :      vjleppert-at-ucdavis.edu
Date: Fri, 7 Apr 2000 14:55:56 -0700 (PDT)
Subject: Mat Sci:Need Tips for Ion Milling Porous Ceramic

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


If anyone has any tips or a reference they could point me to for this, it
would be much appreciated.

We have had several users with nanocrystalline ceramic samples from
combustion or plasma syntheses who have had problems with the thin areas
of their samples falling out during ion milling. We have tried a few
schemes to correct this, without much success. Any help would be greatly
appreciated.

The current user has 2 different nanocrystalline composites: TiB2/TiN and
TiB2/TiC.

Valerie Leppert
Dept. Chem. Eng. and Mat. Sci.
University of California, Davis

vjleppert-at-ucdavis.edu




From daemon Sat Apr 08 08:03:18 2000



From: Bernard Kestel :      kestel-at-anl.gov
Date: 07 Apr 00 16:39:01 -0600
Subject: Horror Prevention:

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Maybe Judy Murphy's Delta College will diminish these "untrained"
occurrences!

Bernie Kestel
Argonne National Laboratory




From daemon Sat Apr 08 08:03:19 2000



From: Cynthia Shannon :      cshannon-at-nctimes.net
Date: Fri, 07 Apr 2000 15:24:09 -0700
Subject: Wanted:Used Zeiss 10c TEM

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Does anyone have a functioning Zeiss 10c TEM that they would like to
donate to a goverment office for a tax deduction or sell for cheap?
I am the electron microscopist for the county veterinarian and we are
trying to locate a Zeiss 10c to be used for virus identification work on
animals and plants.
Thank you.
Cindy Shannon
cshannon-at-nctimes.net




From daemon Sat Apr 08 08:03:19 2000



From: Frederick Schamber :      fhscham-at-sgi.net
Date: Fri, 07 Apr 2000 23:58:40 -0400
Subject: Perfluorinated Polyether Oils and Greases -- are they a problem ?

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This is actually a follow-on question to the thread on "Silicone Oils and
Greases" which ran a few days ago.

In his typically thorough response to the discussion of why microscopists
generally avoid silicone-based vacuum greases, Will Bigelow noted that

} While the silicone high vacuum grease is
} indeed a good lubricant for O-rings, it is no better than the Brayco and
} Krytox greases, which are based on polyphenylether compounds, and which do
} not introduce the possibility of having insoluble siliceous compounds
} formed on critical parts of the electron optical column.
}

This mention of Krytox rang a bell and I checked Chapter 10 of Will's book
"Vacuum Methods in Electron Microscopy" where he discusses oils and greases.
In table 10.1 he lists both Braycote and Krytox as "Perfluorinated Polyether
based" (not polyphenylether). In the subsequent discussion he states that they
"probably should not be used ... in or near the electron gun because of the
possibility that some of the perfluorinated base compound might get onto the
high voltage insulator and cause microdischarges ... ". In chapter 5 he is
more specific in his discussion of perfluorinated polyether diffusion pump oils
where he again mentions Krytox and states that use of these pump oils was
generally abandoned because it was found that "electron microscopes in which
these fluids were used eventually developed high-voltage instabilities due to
micro-discharges along the ceramic insulators in the electron guns." Will goes
on to note that the problem seems to be more severe in TEMs (which operate at
higher voltages) and these fluids "... have been used successfully for several
years in scanning electron microscopes in some laboratories".

First question:
I'm confused by Will's list-server statement that Krytox is a polyphenylether
when it is listed in his book as a perfluorinated polyether. Not being an
organic (or any other kind) of chemist, I might think that these are synonyms
for the same family of materials except that Will also has a separate
discussion of polyphenyl ether pump fluids (e.g., Convalex and Santovac) which
is separate from that of the perfluorinated polyethers (Krytox and Fomblin). I
think that Krytox actually is a perfluorinated polyether, isn't it?

Second question:
The issue of whether Krytox and Braycote are perfluorinated polyethers is more
than a trivial nomenclature issue because of the alleged problem of
perfluorinated compounds contaminating high-voltage insulators. In addition to
the somewhat equivocal cautionary statements in Will's book, I personally know
of one lab which has banished these compounds from the premises because of an
incident a number of years ago where the glassware used for cleaning of
microscope parts got contaminated with Krytox -- this got transferred to
several TEMs and resulted (so I'm told) in the need to replace multiple guns
over a period of time -- and produced a lot of hair-pulling until the source of
the problem was identified (actually, maybe this should be under the "horror
stories" thread?). But this is the only direct report I have heard of such a
problem and Will, though he notes the concerns, doesn't seem reluctant to
recommend the stuff for EM use (and I do respect his depth of experience in
such things). So my question: Is Krytox really the "bogeyman" I've been told
it is? Or is this just another bit of microscopy folklore?

Given that: (1) there are lots of ways a vacuum grease could get transferred to
a gun indirectly (i.e., via tools or contaminated gloves); (2) this stuff is
reported to be essentially impossible to remove once it gets deposited on
something; (3) the purported HV discharge behavior doesn't show up immediately
but develops gradually over time; and (4) insulator cleanliness is enough of a
problem without introducing this kind of sneaky contaminant -- IF true, it
would seem that this class of compounds has no place in an EM lab. But if this
is all a myth, I'm depriving myself of some otherwise great products. Insight
anyone?

Fred Schamber



From daemon Sat Apr 08 08:03:28 2000



From: Steve Chapman :      protrain-at-emcourses.com
Date: Sat, 8 Apr 2000 08:22:26 +0100
Subject: Re: sputter coater

Contents Retrieved from Microscopy Listserver Archives
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Hi

Most sputter coater manufacturers use one of two methods for mounting their
targets.

1. Target material fixed by a clip or adhesive to an aluminium base
(difficult to sputter without a special coater)

2. Target material fixed by adhesive to a brass base (this will sputter
in relation to its composition e.g. 70%Cu 30%Zn)

You seem to have the type (2) target and are probably sputtering a mixture
of gold (from the outside of the target) and brass (from the middle of the
target). Check it out if you have x-ray analysis?

Sputter targets last a period in direct relationship to target thickness,
coating thickness and degree of use, how long is a piece of string?

Good luck

Steve Chapman
Senior Consultant
Protrain - professional training in EM world wide
Tel +44 1280 814774 Fax +44 1280914007
e-mail protrain-at-emcourses.com
web site www.emcourses.com




From daemon Sat Apr 08 08:03:28 2000



From: Steve Chapman :      protrain-at-emcourses.com
Date: Sat, 8 Apr 2000 08:21:38 +0100
Subject: Re: "S" -distortion in TEM images JEOL 2000FX

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi

"S" distortion or anisotropic distortion is produced through a balancing of
aberrations. Take one imaging lens which is being used at low current and
you will have pincushion distortion. Add another lens that is being used in
the low to middle part of its current range and you will have pincushion
distortion. Use the two lenses in a magnification system and the result is
an image with anisotropic distortion.

When we design a TEM we try to balance the lenses so that the degree of
distortion is minimised. Minimised does not mean totally removed it means
NO distortion in the area that falls within the photographic frame. In
early instruments, where the screen was very small, you hardly ever saw the
problem, as screens have increased in size so the problem becomes more
clear.

If the distortion has just started to become annoying this may be due to one
or two reasons.

1) The high voltage has changed such that the lenses are not matched to
the accelerating voltage as they were when the system was new?

2) A lens is not running at the correct level, its current is too low?

The most common reason for a change in lens or high voltage performance are
their reference circuits, I am afraid I am not familiar with these circuits
in the 2000FX.

Good luck hope this helps?

Steve Chapman
Senior Consultant
Protrain - professional training in EM world wide
Tel +44 1280 814774 Fax +44 1280914007
e-mail protrain-at-emcourses.com
web site www.emcourses.com




From daemon Sat Apr 08 08:03:32 2000



From: Terje Dokland :      dokland-at-ima.org.sg
Date: Sat, 8 Apr 2000 10:06:50 +0800
Subject: TEM - Technician position available at IMA, Singapore

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


ELECTRON MICROSCOPIST
Laboratory of Structural Biology
Institute of Molecular Agrobiology, Singapore

A position is available for an experienced EM technician at the
Institute of Molecular Agrobiology (IMA), Singapore, in the group of Dr.
Terje Dokland, Laboratory of Structural Biology. The successful candidate
will be responsible for the operation of the structural EM laboratory, and
be involved in several research projects, in particular structural
characterization of various plant and animal viruses of agricultural
importance. Experience with cryo-EM of frozen-hydrated samples is a
definite advantage, as is some experience with using computers, especially
3D reconstruction methods.
IMA is a well funded and rapidly expanding research institute which
was established in 1995 to conduct basic and applied research in
agrobiology. It moved into a modern and spacious building at the National
University of Singapore campus two years ago, and has state-of-the art
equipment for structural and molecular biology, biochemistry, plant growth,
tissue culture etc. There are currently two groups working in structural
biology, including X-ray crystallography and electron microscopy, and there
is extensive collaboration with other laboratories and institutes, both in
Singapore and abroad. Further information about the institute can be found
at http://www.ima.org.sg. Singapore is a cosmopolitan and multicultural
society offering all the conveniences of a modern city, and is centrally
located in Southeast Asia with easy access to other countries in the
region.
The position will be filled at the Research Officer or Assistant
Research Officer level, depending on experience, and the contract will be
initially offered for two years. IMA offers attractive salaries with bonus
packages and benefits.
Informal enquiries are welcome and can be directed to Terje Dokland
at dokland-at-ima.org.sg. Applications, including a CV and names of two
referees, should be sent to
Terje Dokland
Institute of Molecular Agrobiology
1 Research Link
The National University of Singapore
Singapore 117604


------------------------------------
Terje Dokland
Senior Scientist
Institute of Molecular Agrobiology
1 Research Link
The National University of Singapore
Singapore 117604
Phone: 65-872 7405 Fax: 65-872 7007
E-mail: dokland-at-ima.org.sg

"Convictions are greater enemies of truth than lies"
F. Nietzsche





From daemon Sat Apr 08 08:18:20 2000



From: Frederick Schamber :      fhscham-at-sgi.net
Date: Fri, 07 Apr 2000 23:58:40 -0400
Subject: Perfluorinated Polyether Oils and Greases -- are they a problem ?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


This is actually a follow-on question to the thread on "Silicone Oils and
Greases" which ran a few days ago.

In his typically thorough response to the discussion of why microscopists
generally avoid silicone-based vacuum greases, Will Bigelow noted that

} While the silicone high vacuum grease is
} indeed a good lubricant for O-rings, it is no better than the Brayco and
} Krytox greases, which are based on polyphenylether compounds, and which do
} not introduce the possibility of having insoluble siliceous compounds
} formed on critical parts of the electron optical column.
}

This mention of Krytox rang a bell and I checked Chapter 10 of Will's book
"Vacuum Methods in Electron Microscopy" where he discusses oils and greases.
In table 10.1 he lists both Braycote and Krytox as "Perfluorinated Polyether
based" (not polyphenylether). In the subsequent discussion he states that they
"probably should not be used ... in or near the electron gun because of the
possibility that some of the perfluorinated base compound might get onto the
high voltage insulator and cause microdischarges ... ". In chapter 5 he is
more specific in his discussion of perfluorinated polyether diffusion pump oils
where he again mentions Krytox and states that use of these pump oils was
generally abandoned because it was found that "electron microscopes in which
these fluids were used eventually developed high-voltage instabilities due to
micro-discharges along the ceramic insulators in the electron guns." Will goes
on to note that the problem seems to be more severe in TEMs (which operate at
higher voltages) and these fluids "... have been used successfully for several
years in scanning electron microscopes in some laboratories".

First question:
I'm confused by Will's list-server statement that Krytox is a polyphenylether
when it is listed in his book as a perfluorinated polyether. Not being an
organic (or any other kind) of chemist, I might think that these are synonyms
for the same family of materials except that Will also has a separate
discussion of polyphenyl ether pump fluids (e.g., Convalex and Santovac) which
is separate from that of the perfluorinated polyethers (Krytox and Fomblin). I
think that Krytox actually is a perfluorinated polyether, isn't it?

Second question:
The issue of whether Krytox and Braycote are perfluorinated polyethers is more
than a trivial nomenclature issue because of the alleged problem of
perfluorinated compounds contaminating high-voltage insulators. In addition to
the somewhat equivocal cautionary statements in Will's book, I personally know
of one lab which has banished these compounds from the premises because of an
incident a number of years ago where the glassware used for cleaning of
microscope parts got contaminated with Krytox -- this got transferred to
several TEMs and resulted (so I'm told) in the need to replace multiple guns
over a period of time -- and produced a lot of hair-pulling until the source of
the problem was identified (actually, maybe this should be under the "horror
stories" thread?). But this is the only direct report I have heard of such a
problem and Will, though he notes the concerns, doesn't seem reluctant to
recommend the stuff for EM use (and I do respect his depth of experience in
such things). So my question: Is Krytox really the "bogeyman" I've been told
it is? Or is this just another bit of microscopy folklore?

Given that: (1) there are lots of ways a vacuum grease could get transferred to
a gun indirectly (i.e., via tools or contaminated gloves); (2) this stuff is
reported to be essentially impossible to remove once it gets deposited on
something; (3) the purported HV discharge behavior doesn't show up immediately
but develops gradually over time; and (4) insulator cleanliness is enough of a
problem without introducing this kind of sneaky contaminant -- IF true, it
would seem that this class of compounds has no place in an EM lab. But if this
is all a myth, I'm depriving myself of some otherwise great products. Insight
anyone?

Fred Schamber




From daemon Sat Apr 08 08:18:21 2000



From: Steve Chapman :      protrain-at-emcourses.com
Date: Sat, 8 Apr 2000 08:22:26 +0100
Subject: Re: sputter coater

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi

Most sputter coater manufacturers use one of two methods for mounting their
targets.

1. Target material fixed by a clip or adhesive to an aluminium base
(difficult to sputter without a special coater)

2. Target material fixed by adhesive to a brass base (this will sputter
in relation to its composition e.g. 70%Cu 30%Zn)

You seem to have the type (2) target and are probably sputtering a mixture
of gold (from the outside of the target) and brass (from the middle of the
target). Check it out if you have x-ray analysis?

Sputter targets last a period in direct relationship to target thickness,
coating thickness and degree of use, how long is a piece of string?

Good luck

Steve Chapman
Senior Consultant
Protrain - professional training in EM world wide
Tel +44 1280 814774 Fax +44 1280914007
e-mail protrain-at-emcourses.com
web site www.emcourses.com





From daemon Sat Apr 08 08:18:21 2000



From: Steve Chapman :      protrain-at-emcourses.com
Date: Sat, 8 Apr 2000 08:21:38 +0100
Subject: Re: "S" -distortion in TEM images JEOL 2000FX

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi

"S" distortion or anisotropic distortion is produced through a balancing of
aberrations. Take one imaging lens which is being used at low current and
you will have pincushion distortion. Add another lens that is being used in
the low to middle part of its current range and you will have pincushion
distortion. Use the two lenses in a magnification system and the result is
an image with anisotropic distortion.

When we design a TEM we try to balance the lenses so that the degree of
distortion is minimised. Minimised does not mean totally removed it means
NO distortion in the area that falls within the photographic frame. In
early instruments, where the screen was very small, you hardly ever saw the
problem, as screens have increased in size so the problem becomes more
clear.

If the distortion has just started to become annoying this may be due to one
or two reasons.

1) The high voltage has changed such that the lenses are not matched to
the accelerating voltage as they were when the system was new?

2) A lens is not running at the correct level, its current is too low?

The most common reason for a change in lens or high voltage performance are
their reference circuits, I am afraid I am not familiar with these circuits
in the 2000FX.

Good luck hope this helps?

Steve Chapman
Senior Consultant
Protrain - professional training in EM world wide
Tel +44 1280 814774 Fax +44 1280914007
e-mail protrain-at-emcourses.com
web site www.emcourses.com





From daemon Sat Apr 08 12:21:05 2000



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Sat, 08 Apr 2000 13:08:09 -0500
Subject: Sputter coater cathode

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Joyce Craig wrote:
===================================
How long should a gold target last?
Our target looks gold except in the center.
Problem is that we don't seem to get good coating.
===================================
This is not that uncommon of a question. The first rule of thumb is that if
it does not look like gold, then it probably is not gold. There is one
exception however, and that is when the sputtering process creates some kind
of surface structure that leads to an optical effect, a non-gold looking
gold color (more like grey). But that typically does not adversely effect
the sputtering rate.

The other cases then would be either a) contamination from external sources
(e.g. finger prints) or b) build up of contaminants from the use of gold
with insufficient purity. If (a), then that "problem" is solved by a good
solvent washing and scrubbing with something like acetone. If (b), then it
will not rub off with solvent and it would be something building up in the
way of impurities from within the gold foil itself.

There is a cost associated with taking gold from 0.98 to 0.99 and even more
of a cost to 0.999 purity, then then to 0.9999 or 0.99995 still more cost.
In other words, you really do want high purity gold in the cathodes, for
this very reason, but the higher purities do cost more money. Putting it
another way, a cathode of 0.9999 for example is a lot more expensive than
one that is 0.98 or 0.99, even though the net gold content is about the same
. I won't even begin to speculate on how many would see a difference
between 0.99995 vs. 0.9999 or even 0.999. But there is a point where the
impurity elements that don't sputter begin to build upon the surface,
resulting in a mostly non-gold layer. It is my understanding that the
original equipment manufacturers of sputter coaters and also the main firms
offering replacement cathodes supply only cathodes at the higher end of the
purity scale because of this reason. If you seek non-traditional EM sources
as alternatives, you really have to see the documentation to know that you
are getting high purity, but most of the alternative sources do not deal in
such high purity gold.

If you feel you have followed some of the advice given out on this
listserver about possibly saving money and you could be having a build up of
alloying or contaminating elements, try polishing off this layer in a
metallographic polishing table, in order to renew the original composition
that apparently did work for some time. If you do this, I would be most
appreciative if you could share your experience with the list, be it good or
bad. It might create more of an awareness of the need for high purity gold
when making cathodes.

Disclaimer: SPI Supplies offers replacement gold and other metal cathodes
for sputter coaters used in the SEM laboratory so we would have a vested
interest in customers purchasing their cathodes from SPI or our major
competitors in the EM supplies business.

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.spi.cc
########################
============================================



From daemon Sat Apr 08 15:29:41 2000



From: Mandayam V. Parthasarathy :      mvp2-at-cornell.edu
Date: Sat, 8 Apr 2000 14:08:28 -0400
Subject: CM12 Ultra High tilt holder

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does any one have a good condition Gatan 670 Ultra High Tilt Specimen
Holder (80 deg.) for Philips CM12 for sale?


*******************************************************************

Dr. M.V. Parthasarathy
Prof. of Plant Biology, Adjunct Prof. of Biomedical Sciences (Vet), &
Director, Cornell Integrated Microscopy Center (CIMC)
Department of Plant Biology
228 Plant Science Building
Cornell University, Ithaca, NY 14853
E-Mail: mvp2-at-cornell.edu
Plant Biology Office: 268 Emerson; Telephone: 607-255-1734
Plant Biology Fax: 607-255-5407
CIMC Office: C1 054 Vet. Med. Center; Telephone: 607-253-3803
CIMC Office Fax: 607-253-3803
CIMC web site: http://www.cimc.cornell.edu




From daemon Sat Apr 08 15:29:45 2000



From: Ken Converse :      qualityimages-at-netrax.net
Date: Sat, 08 Apr 2000 14:22:01 -0700
Subject: Re: Perfluorinated Polyether Oils and Greases -- are they a problem ?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Frederick Schamber wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} This is actually a follow-on question to the thread on "Silicone Oils and
} Greases" which ran a few days ago.
}
} In his typically thorough response to the discussion of why microscopists
} generally avoid silicone-based vacuum greases, Will Bigelow noted that
}
} } While the silicone high vacuum grease is
} } indeed a good lubricant for O-rings, it is no better than the Brayco and
} } Krytox greases, which are based on polyphenylether compounds, and which do
} } not introduce the possibility of having insoluble siliceous compounds
} } formed on critical parts of the electron optical column.
} }
}
} This mention of Krytox rang a bell and I checked Chapter 10 of Will's book
} "Vacuum Methods in Electron Microscopy" where he discusses oils and greases.
} In table 10.1 he lists both Braycote and Krytox as "Perfluorinated Polyether
} based" (not polyphenylether). In the subsequent discussion he states that they
} "probably should not be used ... in or near the electron gun because of the
} possibility that some of the perfluorinated base compound might get onto the
} high voltage insulator and cause microdischarges ... ". In chapter 5 he is
} more specific in his discussion of perfluorinated polyether diffusion pump oils
} where he again mentions Krytox and states that use of these pump oils was
} generally abandoned because it was found that "electron microscopes in which
} these fluids were used eventually developed high-voltage instabilities due to
} micro-discharges along the ceramic insulators in the electron guns." Will goes
} on to note that the problem seems to be more severe in TEMs (which operate at
} higher voltages) and these fluids "... have been used successfully for several
} years in scanning electron microscopes in some laboratories".
}
} First question:
} I'm confused by Will's list-server statement that Krytox is a polyphenylether
} when it is listed in his book as a perfluorinated polyether. Not being an
} organic (or any other kind) of chemist, I might think that these are synonyms
} for the same family of materials except that Will also has a separate
} discussion of polyphenyl ether pump fluids (e.g., Convalex and Santovac) which
} is separate from that of the perfluorinated polyethers (Krytox and Fomblin). I
} think that Krytox actually is a perfluorinated polyether, isn't it?
}
} Second question:
} The issue of whether Krytox and Braycote are perfluorinated polyethers is more
} than a trivial nomenclature issue because of the alleged problem of
} perfluorinated compounds contaminating high-voltage insulators. In addition to
} the somewhat equivocal cautionary statements in Will's book, I personally know
} of one lab which has banished these compounds from the premises because of an
} incident a number of years ago where the glassware used for cleaning of
} microscope parts got contaminated with Krytox -- this got transferred to
} several TEMs and resulted (so I'm told) in the need to replace multiple guns
} over a period of time -- and produced a lot of hair-pulling until the source of
} the problem was identified (actually, maybe this should be under the "horror
} stories" thread?). But this is the only direct report I have heard of such a
} problem and Will, though he notes the concerns, doesn't seem reluctant to
} recommend the stuff for EM use (and I do respect his depth of experience in
} such things). So my question: Is Krytox really the "bogeyman" I've been told
} it is? Or is this just another bit of microscopy folklore?
}
} Given that: (1) there are lots of ways a vacuum grease could get transferred to
} a gun indirectly (i.e., via tools or contaminated gloves); (2) this stuff is
} reported to be essentially impossible to remove once it gets deposited on
} something; (3) the purported HV discharge behavior doesn't show up immediately
} but develops gradually over time; and (4) insulator cleanliness is enough of a
} problem without introducing this kind of sneaky contaminant -- IF true, it
} would seem that this class of compounds has no place in an EM lab. But if this
} is all a myth, I'm depriving myself of some otherwise great products. Insight
} anyone?
}
} Fred Schamber


Fred,
I've used Brayco 803 since about 1980 for static seals and have never
had any problems. I used Apiezon L for dynamic seals until I was
introdused to Brayco 602 at which point I noticed cleaner systems once
the Apiezon was gone. Apiezon's vapor pressure numbers look pretty good
until you elevate the temp a little (120F) and then you lose a couple of
decades, if I recall correctly, and the stuff polymerizes like crazy in
an e-beam, but it IS slippery. I've been using the Brayco 602 in gun
translators for a number of years with no problems and much cleaner
guns.

One other thing I've noticed: Old Apiezon oxidizes and turns to a
sticky sludge but the Brayco greases don't seem to age at all.

Ken Converse
owner
Quality Images
Delta, PA 17314
717-456-5491



From daemon Sun Apr 09 09:28:04 2000



From: Thomas Litzinger :      thomas_c_litzinger-at-yahoo.com
Date: Sun, 9 Apr 2000 09:09:41 -0500
Subject: Newt retina and lens

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I am an undergrad from Miami University of Ohio, and
am beginning TEM research with Newt retina and lens.
I have researched various sources to no avail.
Luckily, I was introduced to your network of
Microscopists.
} } My main problem is finding a thorough protocol
for sample preparation that includes such details as
primary fixation, buffers, resin-type, and accurate
times/temperatures. Using the limited knowledge I
have aquired I was able to put together a experimental
protocol, which is polymerizing at this moment. I
would greatly appreciate any advice or leads on proper
protocol in this area.
} }
} }

} Thanks,
} } Thomas Litzinger

__________________________________________________
Do You Yahoo!?
Talk to your friends online with Yahoo! Messenger.
http://im.yahoo.com




From daemon Sun Apr 09 09:28:04 2000



From: baddis-at-olypen () (by way of Nestor J. Zaluzec)
Date: Sun, 9 Apr 2000 09:12:08 -0500
Subject: Image grabbing with a mieji trinoc

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Email: baddis-at-olypen
Name: barnett addis
Question: I am a retired Phd from another remote disipline in another era
who has become interested im micromount minerals and wish
to do some image grabbing with a mieji trinoc(on order) but have reached
a quandry re cameras. Has anyone had any hands on experience
with a pixeria or a kodak mds100.or other lower priced rigs. i am loking
at these
as affordable options that may provide a better image than the tried and true
method of a color ( below $1,000) video camera and "snappy" as the grabber.
Any comments, rumors, suggestions would be most appreciated

---------------------------------------------------------------------------




From daemon Mon Apr 10 08:15:22 2000



From: richard.beanland-at-gecm.com
Date: Mon, 10 Apr 2000 09:42:54 +0000 (GMT)
Subject: Re: Ungluing M-bond

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Ian,
the only really successful way to get rid of epoxy is to use fuming nitric or sulphuric. You can also try aggressive solvents like tetrahydrofuran or dimethylsulfoxide. What will work usually depends upon the composition of your sample - if it also etches in these acids, you may have difficulties. I can get you some recipes from the books on de-encapsulation of silicon chips we have here, if you like.

All these chemicals (particularly fuming nitric) are very unpleasant and need special precautions for use. Swelling of the epoxy is inevitable, and if the specimen is delicate you may need to go through several cycles of nitric/solvent to remove the epoxy without putting too much stress on your sample.

Richard



} Dear all,
} I have made a cross section of something using M-Bond 610 resin and it
} shifted in the clamp while curing so I now have a cross section that would
} be almost impossible to polish to leave the interface vertical. I would
} prefer not to just throw the piece in the bin and start again, due to lack
} of material. Does anyone know a way to dissolve fully cured M-bond 610 so
} that I could start again from scratch?
}
} Thanks
}
} Ian MacLaren
} Beijing Laboratory of Electron Microscopy
} Chinese Academy of Sciences
} P.O. Box 2724
} 100080 Beijing
} China
} General Email: ian.maclaren-at-physics.org
} Work (esp. large attachments): maclaren-at-image.blem.ac.cn
}
} ______________________________________________________
} Get Your Private, Free Email at http://www.hotmail.com

==============================================================
Richard Beanland
Caswell Technology,
Caswell,
Towcester,
Northants NN12 8EQ

e-mail richard.beanland-at-gecm.com
Tel. +44 1327 356363
Fax. +44 1327 356398
==============================================================
"The information contained in this message is legally privileged and confidential information intended for the eyes of the individual or entity named above. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message is strictly prohibited. If you have received this message in error, please notify us immediately by telephone.
Caswell Technology is the trading name of Marconi Caswell Limited. Registered in London No. 3694360 Registered Office: One Bruton Street London W1X 8AQ. Holding Company: Marconi plc."






From daemon Mon Apr 10 08:15:22 2000



From: Keith Ryan :      KPR-at-wpo.nerc.ac.uk
Date: Mon, 10 Apr 2000 10:10:02 +0100
Subject: folds in sections - summary (long-ish)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello everyone

I had quite a few suggestions for getting rid of creases and wrinkles
in semi-thin sections - as promised to some of you, here is a summary.
At the end, i have appended some old references which I dug out of my
store over the weekend. i don't know if they all/if any work! I am
still trying. If I get good results, I may post another message!
Meanwhile, on with the folk-lore!

And waiting for the moon to rise!

Keith Ryan
Marine Biological association
Plymouth UK

Folds in semi-thin sections


1. Use lower drying/staining temperatures - e.g. 60 C - when
hotplate is still warming up
2. Dry flat at 50-55 C then go to hotter plate for 10-15 minutes
3. Longer on the hotplate
4. Dry sections on slide using a flame
5. Set hotplate to just below boiling the water
6. Vapour (6 messages)
7. Transfer to 10% acetone droplets
8. Harder resin (2 messages)
9. Softer resin
10. Longer infiltration times
11. Improper mixing
12. Heat pen (3 messages)
13. Chien grids (grid with 2 mm hole and tab for handling) to
transfer thicks to water droplets
14. Wick away the water once section is spread
15. Use big drops, lots of water
16. Cut thinner sections
17. Cleanliness - of all items
18. Stain on drop of stain on hotplate, steam, transfer, water
rinses, dry down
19. Put a nick in the corner of the cutting face so that the resin
can expand differentially to the enclosed specimen
20. Etch the sections with ethoxide (ethanol + NaOH)
21. Remove resin to lessen apparent effect? Using saturated NaOH in
methanol.

22. Coat the slide - One droplet of protein-glycerol is diluted in 1
ml dist. water, then the glass slide is dipped into this solution and
dried in an oven at 30 C. Protein-glycerol: dissolve 1 gram ALBUMIN
(MERCK) in 9 gram bidist. water at 37xC in an oven. Filter the
solution and mix with the same amount of glycerol.


23. W.M. Harris (1978). Stain Technol. 53: 298-300.
Transfer sections to a large drop of 10% acetone. Dry on hotplate
set to 122 C (250 F). It says that! If the sections are known to be
difficult to flatten, add more 10% acetone during the process. Remove
the sections from the hotplate as soon as they are dried down.
EXCESSIVE HEAT AFTER DRYING DOWN CAUSES WRINKLES. Finally, [place
slides on a warming tray at 40-50 C and allow to dry down thoroughly,
30 minutes to overnight. Then stain.

24. M. Martins-Green (1978). Stain Technol. 53: 296-300.
Sections spread with xylene vapour. Floated on toluidine blue stain
(diluted, 1 ml of 1% stain plus 20 mls 2.5% sodium carbonate sol.) at
50 C on hotplate for 1 hour, then left in the dark at room temperature
for 24 hours). Rinsing - not mentioned. Dried on 2-3 drops of
double-distilled water for 10 minutes at 50 C. Cover-slipped. Method
designed to allow collagen fibres to fully distend after cutting.


25. J.R. Sommer et al. (1979) Stain Technol. 54: 106-107
"wrinkles appear during staining rather than while the sections are
being dried on the glass slides - eliminated when the stain is made
up with 50% with respect to glycerol. Staining is not rapid - 12-24
hours in a covered dish at 50 C. Slides need not be albuminized"
Imply staining by flotation at 60 C in a covered dish 12-24 hours,
rinse briefly, dry on slides and view (without a coverslip).


26. J. Millonig (1980) Stain Technol. 55: 118-120.
Paraphrasing: Wrinkles appear when sections are heated during
staining, more so when there is a rim of resin around the tissue.
During heating, the tissue expands more than the tissue. Transfer
sections onto a drop of stain on the end of a slide. Heat in a flame.
Place on a staining bridge (usually 2 minutes). After cooling, float
sections off on water. Rinse in another beaker, transfer to acidified
water. Transfer to slide, warm in a flame, wick away excess water. Do
not need to albuminise the slide. 0.5 micron sections stain in abouit
2 minutes, thinner sections need a second heating and should be left
floating on stain for longer.


27. N.B. Chandler & G.C. Schoenwlf (1983). Stain Technol. 58:
238-240.
State that "wrinkling occurs only when mounted sections are *.
heated".
Sections dried overnight at 76 C on acid cleaned slides, prior to
staining (76 C was the maximum temp. of their hotplate). Acid
cleaning: soak slides for a minimum of 1 hour in 10% potassium
dichromate plus 10% conc. sulphuric acid. Sections dried at 60 C
often wrinkled, while those dried at 90 C often failed to stain
properly. Sections dried less than 6 hours at 76 C often wrinkled
during staining - hence dry overnight. Staining can be controlled
more consistently in Coplin jars placed in a water bath rather than on
a hotplate.


That's all, folks
except for "Hello, Daniele"!




From daemon Mon Apr 10 08:15:23 2000



From: Frank Thomas :      thomasf-at-AGC.BIO.NS.CA
Date: Mon, 10 Apr 2000 08:14:03 -0300
Subject: Re: and another horror story

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My horror story is pretty recent: Just last January. We had a local SEM
service guy come in to do a semi-annual tune-up on our ESEM, and he did a
great job. He did notice, though, that our ion pump wasn't giving us quite
as good a vacuum as it should, and was kind of slow doing it. When he left
he said "All you need to do is bake it off a bit with some heat for a few
hours - that'll refresh the active ingredient (or parts) inside and it'll
work better for you. I've got a heater that's designed for just such a
job."
True to his word, a couple days later he dropped off this device for me.
Just a biggish aluminum box in two parts, with luggage clips to lock it
together and a 1500 W heater element inside. Simplicity itself to use, just
turn off your ion pump, clip this box over/around it, and plug it in for a
while. The heater element rests right against the back of the ion pump. So
I mount it on there, plug it in, wait a bit, and sure enough, things start
heating up in there. OK, I think, I'll go see my colleague upstairs for a
few minutes about those samples she was preparing.....
I'm back down in the lab ten minutes later, and go back behind the scope
to see how things are doing. There's a small hole in the back of the heater
apparatus, and when I happen to glance in there, I see a small wall of blue
flame. "This can't be good", thinks I....then "Now which type of fire
extinguisher do you use on a burning ESEM? Water?......No, probably not.
Powder?......uhhhhh.......no. CO2? Probably..." Meanwhile, I unplug the
heater and mostly just stand there....thinking how close I've come to
pensionable retirement age, only to lose it like this.
But anyway, the flame started to die back a bit as soon as I unplugged the
thing, and with some damp towels I was soon able to unclip it and remove
it. It turns out our particular instrument had a clip mounted on the back
of the ion pump to retain the HT cable in place, and this clip had been
mounted on piece of black plastic, which I swear to God I thought was
anodized aluminum. The plastic had melted with all that intense heat of
course, and dripped right down onto the heater element, where it had burst
into flame.
I had our machine shop make me a metal replacement for the lost plastic
bit, there was no other damage, by God, the ion pump now works great, and I
figure local management doesn't need to hear about every little detail of
life in the SEM lab, now, do they?


Frank Thomas
MicroAnalysis Facility
Geological Survey of Canada (Atlantic)
Bedford Institute of Oceanography
Dartmouth, Nova Scotia



From daemon Mon Apr 10 08:15:24 2000



From: Dr Malcolm Roberts :      malc-at-rock.ru.ac.za
Date: Mon, 10 Apr 2000 12:34:49 +0200
Subject: Sludge analysis

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G'day Cobbers!
I could use some handy hints here. We've a student who wishes to
analyse some hideous sludge - something to do with sewage me thinks. She
is particularly interested in the state of the metals in this muck -
whether it is present as sulphides, sulphates, nitrides, nitrates or
bound to organic molecules. The stuff is rather fine grained, and I've
suggested she filter and retain the } 10 micron fraction. She will then
press it into a pellet with a flat, shiney surface if all goes well.
Basically, I'm interested if there is anyone out there who has
experience of analysing this type of material. What did you coat it
with? How did you differentiate between the the various form of the
metal compounds? Any other handy hints also gratefully received.
Cheers,
Malc.

--
Dr MP Roberts Phone: [+27](0)46 603 8313
Dept of Geology Fax: [+27](0)46 622 9715
Rhodes University Cell: 083 4060 262 (usually off)
6140 Grahamstown e-mail: malc-at-rock.ru.ac.za
SOUTH AFRICA




From daemon Mon Apr 10 08:15:25 2000



From: John Heckman :      heckman-at-pilot.msu.edu
Date: Mon, 10 Apr 2000 08:12:01 -0700
Subject: Re: Ungluing M-bond

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Hi Ian,

I'm not sure what the Measurements Group uses to cross-link the M-Bond gage cement. The resin is listed as an "epoxy-phenolic adhesive". In several anhydride cross linked systems I've found that sodium ethoxide in ethanol worked well to de-resin samples. I made mine fresh from sodium and ethanol but fresh anhydrous AR grade should work as well. Something like 5 or 10% in dry ethanol should do it. This may be
easier on your samples if they are acid sensitive. I'd be curious what works as I can see myself in a similar predicament someday.
cheers,
John

John Heckman
MSM Department
Michigan State University

richard.beanland-at-gecm.com"-at-sparc5.microscopy.com wrote:

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} Hi Ian,
} the only really successful way to get rid of epoxy is to use fuming nitric or sulphuric. You can also try aggressive solvents like tetrahydrofuran or dimethylsulfoxide. What will work usually depends upon the composition of your sample - if it also etches in these acids, you may have difficulties. I can get you some recipes from the books on de-encapsulation of silicon chips we have here, if you like.
}
} All these chemicals (particularly fuming nitric) are very unpleasant and need special precautions for use. Swelling of the epoxy is inevitable, and if the specimen is delicate you may need to go through several cycles of nitric/solvent to remove the epoxy without putting too much stress on your sample.
}
} Richard
}
} } Dear all,
} } I have made a cross section of something using M-Bond 610 resin and it
} } shifted in the clamp while curing so I now have a cross section that would
} } be almost impossible to polish to leave the interface vertical. I would
} } prefer not to just throw the piece in the bin and start again, due to lack
} } of material. Does anyone know a way to dissolve fully cured M-bond 610 so
} } that I could start again from scratch?
} }
} } Thanks
} }
} } Ian MacLaren
} } Beijing Laboratory of Electron Microscopy
} } Chinese Academy of Sciences
} } P.O. Box 2724
} } 100080 Beijing
} } China
} } General Email: ian.maclaren-at-physics.org
} } Work (esp. large attachments): maclaren-at-image.blem.ac.cn
} }
} } ______________________________________________________
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} ==============================================================
} Richard Beanland
} Caswell Technology,
} Caswell,
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}
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} Tel. +44 1327 356363
} Fax. +44 1327 356398
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} Caswell Technology is the trading name of Marconi Caswell Limited. Registered in London No. 3694360 Registered Office: One Bruton Street London W1X 8AQ. Holding Company: Marconi plc."



From daemon Mon Apr 10 08:15:27 2000



From: anderron-at-us.ibm.com
Date: Mon, 10 Apr 2000 08:51:26 -0400
Subject: Sludge analysis

Contents Retrieved from Microscopy Listserver Archives
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A microprobe wouldn't be my first choice for analyzing the sludge.
Essentially, you'd wind up with a list of elements. Old fashioned x-ray
diffractometry would yield a list of PHASES, which would be a much greater
help. A microprobe's list of elements would, of course, be a great aid in
the x-ray diffraction search/match operation. Check out the International
Centre for Diffraction Data's web site for the latest computer
search/matching things. www.icdd.com (or is it .org?)

Ron

Cobber?

(I have no financial interest in the ICDD)


Ron Anderson, IBM, Hopewell Jct., New York, USA. anderron-at-us.ibm.com

IBM Analytical Services; http://www.chips.ibm.com/services/asg



Dr Malcolm Roberts {malc-at-rock.ru.ac.za} on 04/10/2000 06:34:49 AM

To: Microprobe discussion group {Microscopy-at-sparc5.microscopy.com}
cc:


G'day Cobbers!
I could use some handy hints here. We've a student who wishes to
analyse some hideous sludge - something to do with sewage me thinks. She
is particularly interested in the state of the metals in this muck -
whether it is present as sulphides, sulphates, nitrides, nitrates or
bound to organic molecules. The stuff is rather fine grained, and I've
suggested she filter and retain the } 10 micron fraction. She will then
press it into a pellet with a flat, shiney surface if all goes well.
Basically, I'm interested if there is anyone out there who has
experience of analysing this type of material. What did you coat it
with? How did you differentiate between the the various form of the
metal compounds? Any other handy hints also gratefully received.
Cheers,
Malc.

--
Dr MP Roberts Phone: [+27](0)46 603 8313
Dept of Geology Fax: [+27](0)46 622 9715
Rhodes University Cell: 083 4060 262 (usually off)
6140 Grahamstown e-mail: malc-at-rock.ru.ac.za
SOUTH AFRICA








From daemon Mon Apr 10 17:51:24 2000



From: Dick Briggs :      rbriggs-at-Science.Smith.edu
Date: Mon, 10 Apr 2000 10:31:10 -0500
Subject: Critical Point Drying

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We are attempting to critical point dry 100 micron thick Vibrotome
cut cross sections of annelids (leeches). The end product looks fine
in the SEM, except that the sections have curled/rolled up during the
process, and we want them to remain flat. Attempts to flatten them
after drying have not been very successful. Has anyone devised some
sort of holder (sandwich?) that might overcome this problem by
keeping them flat during their trip through the dryer?

We thank you in advance.

Dick Briggs
Biology Department
Smith College



From daemon Mon Apr 10 17:51:25 2000



From: HILDEGARD CROWLEY :      hcrowley-at-du.edu
Date: Mon, 10 Apr 2000 11:49:23 -0600 (MDT)
Subject: Wrinkles in semi-thins

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This worked for a student a few months back working on nanoporous zirconia.

Ion mill the sample from one side long enough to remove the polishing damage
from that side. Remove from the ion mill and coat the milled side with
carbon. Return to the Ion mill and mill on the uncoated side. The carbon
on the other side will help keep the thinned grains from falling out.

I got this trick from Scott Walck some time ago.

Ray




*************************
Ray D. Twesten, PhD
Center for Microanalysis of Materials
University of Illinois
(217) 244-6177 fax:(217) 244-2278

----- Original Message -----
} From: "Valerie Leppert" {vjleppert-at-ucdavis.edu}
To: "'Microscopy Listserver'" {Microscopy-at-sparc5.microscopy.com}
Sent: Friday, April 07, 2000 4:55 PM



Hi,

If you give me a worm and tell me to produce semi-thin sections that
wrinkle, and semi-thin sections that do not wrinkle, I can do it. But I
might need two worms!

Stretching, hot plate and water manipulations, cutting contortions,
cleanliness, etc., are the band-aids. These methods do not address the
basic problems of which there are at least two.

At the very base of the problem usually lies the fact that there is too
high of a percentage of unbound monomers in the tissue. This can happen
in at least several ways 1) The use of a good formulation which is not
suitable for the tissue 2) the use of a formulation which has no basis in
correct composition as far as molecular weights and WPE #s are concerned.
These formulations are usually handed down from lab to lab and no one
knows their origins. 3) inadequate infiltration procedures of a good and
suitable formulation. All of the above will leave too many unbound
monomers in the tissue.

So what? Unbound monomers are extremely active. They attract water,
swell, pull, stretch the embedded tissue. Upon drying the water leaves
since it is mechanically bound, and presto, nice wrinkles.

Let us look at 1) above. A formulation containing Araldite, and a hefty
amount of DDSA is unsuitable for tough skin, collagen, worm architecture,
and so on. Why? Because Araldite and DDSA are very long chains, and are
extremely difficult to keep EVENLY mixed and EVENLY infiltrated into
tough tissue. Note the capitalization of evenly! Some of the Araldite
will stay outside of the worm gut, and there will be more there than
inside the gut. Inside the gut there is an excess of DDSA. Crosslinkage
and stability are reduced. Water will be absorbed in the boat by these
loose monomers.

I cannot deal with 2) at all. I never use any formulation unless I
understand it. No telling what the proportions are!

Let us look at 3). Inadequate infiltration procedures account for more
trouble than than Monica Lewinsky! Formulations seperate, are allowed to
infiltrate unevenly, or not thoroughly enough, or not slowly enough.
Perhaps the dehydration was not adequate, and the formulation will not
take up a space occupied by a polar substance. Again, you have loose
monomers which should be bound or crosslinked.

What would I do with my worms? For wrinkles I would use
Araldite-DDSA-Epon, infiltrate it poorly, underpolymerize it, and watch
the pleats appear. For flat sections I would pick a formulation which
would fit the material - perhaps the next to the hardest Luft's
formulation, infiltrate it like crazy, never let it sit in the hood, heat
every new change of new mixture to 37 degrees for one hour and a half with
a light bulb while they tissue rotates, do many changes over perhaps 48
hours, at least. I would have really dehydrated well, gone to Propylene
oxide, the infiltrate starting with a mixture of 3 parts propylene oxide
and one part formulation, gradually working up to full epoxy mixture.

I would polymerize for 48 hours at least and cut the thinnest section I
could tolerate for my study. I would use a sharp knife (of course).

Now, it might not work well the first time. So I would manipulate all the
above until I was happy.

NOW. PLEASE NOTE: Suppose I have sections which are a little wrinkled,
or some old blocks which yield severely wrinkled sections. I soak the
slides in rt water for half an hour or less depending on how well they
will stick to the slide. Then I would put the slides into a vaccum jar
which has that strong, powdered dessicant which we used to use for drying
EM film in a petri dish in the bottom. (Protect the slides from flying
poweder by covering with hardened filter paper). Then evacuate the jar
just short of implosion! Release the vaccum in about 24-48 hours. This
will often save poor blocks or totally get rid of slight wrinkles.


Crowley, Hildegard Heinrich, and Ben H. Leichtling. Elimination or
Reduction of Wrinkles in Semithin Epoxy Sections by Vaccum Drying. Stain
Technology,Vol 64, No 5, September 1989.

Please do not ask me for reprints. I do not have any.

Have fun with the worms! Keep records! Know what you have done, and what
you have not done!

Hildy

P.S. Very important! Formulations made from epon substitutes which
contain Araldite or dilutants may not be suitable for use with difficult
materials, since they may be too resilient or elastic. We have used
Eponate 12 from Pella forever. Many years ago I had information regarding
the spec readings of this substitute - it was very much like the old Epon.
I am hazy on this point. Do not quote me. I do not have any monetary
interests in Pella.



From daemon Mon Apr 10 17:51:27 2000



From: Barbara Foster :      mme-at-map.com
Date: Mon, 10 Apr 2000 15:56:19 -0400
Subject: Re: Sludge analysis

Contents Retrieved from Microscopy Listserver Archives
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Dear "Malc",

I'd suggest you also do so good old fashioned light microscopy on a "sludge
smear". We've had the opportunity to use McCrone's Particle Atlas on CD
ROM on several courses recently and have found it invaluable in identifying
things like this. McCrone was running a special at PITTCON: $700 instead
of the usual $900. The last time the PA came out in print, it was
approximately 10 volumes in length. The CD has light, electron, and
elemental analysis info. I would be surprised if there isn't a copy of
either the print or CD version in your library.

Caveat: MME has no financial interest in this product.

Good hunting!
Barbara Foster,President
Microscopy/Microscopy Education
125 Paridon Street Suite 102
Springfield, MA 01118-2130
PH: 413-746-6931 FX: 413-746-9311 email: MME-at-map.com
Website: www.MME-Microscopy.com/education

America's first national consortium of microscopy specialists offering
customized on-site training in all areas of microscopy, image analysis, and
sample preparation
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^




At 12:34 PM 4/10/00 +0200, Dr Malcolm Roberts wrote:
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From daemon Mon Apr 10 17:51:28 2000



From: Karen Rethoret :      rethoret-at-yorku.ca
Date: Mon, 10 Apr 2000 16:05:21 -0400 (EDT)
Subject: Toronto Area Position wanted

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I am writing this on behalf of an experienced SEM Technician, new to
Canada who is looking for a part or full time position, (will even work as
a volunteer for now) in the Greater Toronto Area.

She has 20 years of experience working on various materials on JEOL SEM's
in Hungary but needs help in establishing contacts and learning the
industry here.

Please contact me off-line if you are interested.

Karen Rethoret
York University Microscopy Facility
Toronto, Ontario
416-736-2100 x33289



From daemon Mon Apr 10 17:51:29 2000



From: William Tivol :      tivol-at-wadsworth.org
Date: Mon, 10 Apr 2000 17:18:14 -0400
Subject: Re: Sludge analysis

Contents Retrieved from Microscopy Listserver Archives
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Dr Malcolm Roberts wrote:

Dear Malcolm,

} I could use some handy hints here. We've a student who wishes to
} analyse some hideous sludge - something to do with sewage me thinks. She
} is particularly interested in the state of the metals in this muck -
} whether it is present as sulphides, sulphates, nitrides, nitrates or
} bound to organic molecules.

I reported on using EDS to localize PCBs in sediment at the
Microscopy and Microanalysis meeting in 1998 (page 498 in the
proceedings). Fortunately for me, there is no Cl in sediments, so
just locating Cl was indicative of PCBs. Your student could get
chemical info from microanalysis with very high energy resolution
by looking at the fine structure. Either EELS (on an instrument
with a FEG) or WDS could have this resolution, and if the new
technologies of superconducting tunnel junction diodes or micro-
calorimeter detectors are available, they could provide sufficient
resolution for EDS. Taking position-tagged spectra on, e.g., a
Zeiss 912 could give her both the localization and chemical info
she needs. I have no interest in either Zeiss or the new types of
detector except as a would-be user and techno-geek.


} The stuff is rather fine grained, and I've
} suggested she filter and retain the } 10 micron fraction. She will
} then press it into a pellet with a flat, shiney surface if all goes well.

I just suspended the sediment, put a small amount on a grid,
and took images and spectra. I used all but the mm-sized particles;
most were in the micron to submicron size range. They were well-
dispersed on the grid when the dilution of the suspension was ap-
propriate, and I could get spectra from individual particles. This
approach may be better than the pellet, since different particles
can have different chemistries.


} Basically, I'm interested if there is anyone out there who has
} experience of analysing this type of material. What did you coat it
} with?

I didn't coat it; the high-voltage microscope is capable of
getting good images from this material as is (no charging). The
IVEM, likewise, was able to get good spectra.


} How did you differentiate between the the various form of the
} metal compounds? Any other handy hints also gratefully received.

As I said, I didn't need to get chemical info, and as another
poster said, using diffraction to characterize the material can add
info about the form the metal is in. Reflection high-energy elec-
tron diffraction (RHEED), low-energy electron diffraction (LEED),
and photoelectron holography (PEH) can give info about surface
structures (see, e.g., Leslie et al. (1999) Electron Crystallography in
Surface Structure Analysis, Microscopy Research and Technique
46:160-177). If the metals are adsorbed to the sludge by adhering
to a facet with a particular crystallographic orientation, either as
occasional adatoms or, better still, as an epitaxial deposit, such
surface techniques could be useful. Perhaps Larry Marks is the
best person to talk to about this. Good luck.
Yours,
Bill Tivol




From daemon Mon Apr 10 17:51:29 2000



From: Wil Bigelow :      bigelow-at-engin.umich.edu
Date: Mon, 10 Apr 2000 16:34:13 -0400
Subject: I GOOFED on Brayco & Krytox

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My profound apologies to everyone who read my comments on silicone oils and
greases, in which I said that Brayco and Krytox greases are based on
polyphenyl ether compounds. I was in too much of a hurry, and neglected to
check my memory (which isn't getting any better as my maturity progresses).


Fred Schamber is right in calling attention to the fact that these greases
are based on perfluorinated polyether compounds.

Thus, the information given on page 460 of my book 'Vacuum Methods in
Electron Microscopy' is correct: the Brayco and Krytox greases are based
on perfluorinated polyether compounds, NOT polyphenyl ether compounds.

Furthermore, Fred is correct in stating that great care should be exercised
to keep the perfluoro-polyether compounds out of the electron guns of
electron microscopes, because, as discussed on p. 187 of Vac. Meth. in EM,
they have been known to cause microdischarges in electron guns causing
undesirable high-voltage instabilities.

Although I have not bothered to try to keep up with all new developments
since I finished writing my book, back in '94, I am not aware of any vacuum
grease that is based on polyphenylether fluids. This is peculiar, because
the polyphenyl ether diffusion pump fluids, Santovac-5, Excello-54, and
Convalex-10, have all worked out so well. A grease is made by combining an
oil with a gel-forming agent. Thus, to make an ordinary lubricating grease
one might mix a soap such as sodium stearate with an ordinary lubricating
oil. When heated to a proper temperature the oil and soap will interact to
form a gel, which we call a grease. Thus, to make a grease based on the
polyphenyl ether oils, all one would have to do is to find the appropriate
gelling agent. This is not an entirely trivial task, however, because
unless the correct agent is found the grease will 'bleed' (i.e. the oil
will separate from the gelling agent), especially if the grease is heated
or exposed to a vacuum. If a grease bleeds, you can end up with only the
gelling agent remaining on the bearing or gasket that you wanted to
lubricate, while the oil (which is the actual lubricating agent) spreads
around and contaminates adjacent parts. The silicone- and perfluorinated
polyether-based greases are remarkably stable and resistant to bleeding,
whereas some other high vacuum greases are not.

Again, I apologize for my mistake.

Wilbur C. Bigelow, Prof. Emeritus
Materials Sci. & Engr., University of Michigan
Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu;
Fx:734-763-4788; Ph:734-662-5237




From daemon Mon Apr 10 17:51:32 2000



From: Debby Sherman :      sherman-at-btny.purdue.edu
Date: 10 Apr 2000 17:19:23 -0500
Subject: Re: sputter coater target

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I have also had an interesting, and aggravating problem with sputter coater targets recently. We have an older model Hexland (bought out by Oxford about 10 years ago) SEM cryo stage. Although not as fancy as current models, it usually does a reasonable job. Quite a number of years ago I purchased a large gold disc sputter coater target. I cut the 12mm discs needed for the sputter coater in the cryo prechamber from this larger disk with a simple stamping tool. This worked for years without problems.
About a year or so ago, we started having problems getting good coatings...noticable because of excessive charging of coated samples. We immediately thought of contamination because the target was becoming discolored after only a couple of uses. If I removed it, cleaned it with metal polish, etc and reinstalled it, all was well for another couple of coatings. We tried new pumps, looking for oil leaks etc. but a good vacuum in the chamber ruled out large leaks and all other signs were negative. We also changed the argon tank thinking that perhaps this could be a source of contamination.
The cryo stage is used in spurts and after a period of inactivity, we are again gearing up for heavier use. Recently the target was only giving a good coating on one sample before having to be cleaned....a major job when you have to warm to room temp from -170oC prior to opening up the chamber. I finally took the used target over to a Microprobe on campus and did a WDS analysis on it....turns out that the contamination was aluminum. The housing that holds the target in place is aluminum. Somehow the aluminum is being sputtered as well as the gold, producing a poor metal coating and contaminating the target as well.
Oxford is trying to get replacement parts for the sputter head but we are at a loss to figure out why, after all these years, this is happening and what we can do about it if replacement parts cannot be found.
By the way, a new crystage runs about $70,000 so we are not contemplating that move at the moment.
Debby


Debby Sherman, Manager Phone: 765-494-6666
Microscopy Center in Agriculture FAX: 765-494-5896
Dept. of Botany & Plant Pathology E-mail: sherman-at-btny.purdue.edu
Purdue University
1057 Whistler Building
West Lafayette, IN 47907-1057
On Saturday, April 8, 2000, Garber, Charles A. {cgarber-at-2spi.com} wrote:
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From daemon Mon Apr 10 18:01:36 2000



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Mon, 10 Apr 2000 18:55:45 -0500
Subject: M-Bond 610

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-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Ian MacLaren wrote:
=======================================================
} Dear all,
} I have made a cross section of something using M-Bond 610 resin and it
} shifted in the clamp while curing so I now have a cross section that would

} be almost impossible to polish to leave the interface vertical. I would
} prefer not to just throw the piece in the bin and start again, due to lack

} of material. Does anyone know a way to dissolve fully cured M-bond 610 so

} that I could start again from scratch?
}
} Thanks
}
} Ian MacLaren
} Beijing Laboratory of Electron Microscopy
} Chinese Academy of Sciences
} P.O. Box 2724
} 100080 Beijing
} China
} General Email: ian.maclaren-at-physics.org
} Work (esp. large attachments): maclaren-at-image.blem.ac.cn
=============================================================
We have found over the years that the easiest way to remove not just M-Bond
610 but just about any epoxy or other organic polymer from a metal substrate
is with the use of oxygen plasma etching. It was my recollection that one
of our systems was purchased by the KYKY part of your facility and they
would probably let you have access to it.

Disclaimer: SPI Supplies manufactures the Plasma Prep II plasma etcher
which can be used to remove organics, including intractable cross-linked
polymer systems, like M-Bond 610.

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.spi.cc
########################
============================================




From daemon Mon Apr 10 18:11:35 2000



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Tue, 11 Apr 2000 11:00:47 GMT+1200
Subject: Cobber

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}
} Ron
}
} Cobber?
}

Cobber = Bro = Mate = Buddy

rtch (another cobber from the Southern half of the world)

Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand



From daemon Tue Apr 11 17:10:45 2000



From: Ronald Vane :      RVaneXEI-at-concentric.net
Date: Monday, April 10, 2000 5:26 PM
Subject: Re: sputter coater target

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Dear Debby:

Here is my guess about what has happened. I read someplace that alumina
(aluminum oxide) does not sputter easily whereas pure uncoated aluminum
metal sputter easily. Aluminum in air is quickly coated with alumina and it
is protected for a while when placed in an area subject to ion bombardment
for sputtering. The alumina layer on your target holder has been worn away
by years of sputtering and cleaning, and in the cold vacuum of the cryo
prechamber was not getting replaced by oxidation.

Cure: Take the holder out and have it anodized (a hard finish) or just heat
it gently in air for a while to form a new layer of aluminum oxide.

Ronald Vane
XEI Scientific


-----Original Message-----
} From: Debby Sherman {sherman-at-btny.purdue.edu}
To: message to: MSA list {microscopy-at-sparc5.microscopy.com}



From daemon Tue Apr 11 17:10:52 2000



From: Gordon Couger :      gcouger-at-rfdata.net
Date: Tue, 11 Apr 2000 01:17:20 -0500
Subject: Re: sputter coater target

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Debby,

Could the alumium have had some kind of coating to keep it from sputtering
or could a short developed between the gold disk and the alumium
housing? My guess is that there is a break down in the insulation between
the target and the holder. If you have records of the current and voltage
it sould show up when compaired with the old records when things were
running right.

Could some other part of the system be contaminated by Al? If the
rest of the system is not made of alumium you can use lye to clean
it.

When a new machine costs $70 K you can afford to pay a good machinest
for a lot of hours to make a part. With CAM mills they can make almost
anything. And for what the computer controled mill can't make there are
a few of us old guys that can finish the job with a file:) You porbably have
a couple in one of the insterment shops on campus.

If you don't have a shop that can handle this kind of work I know a couple
of guys out on the west coast that can. I don't have any connection with
them except to admire their work.

Gordon

Gordon Couger gcouger-at-couger.com

Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00


}
} I have also had an interesting, and aggravating problem with sputter
coater targets recently. We have an older model Hexland (bought out by
Oxford about 10 years ago) SEM cryo stage. Although not as fancy as current
models, it usually does a reasonable job. Quite a number of years ago I
purchased a large gold disc sputter coater target. I cut the 12mm discs
needed for the sputter coater in the cryo prechamber from this larger disk
with a simple stamping tool. This worked for years without problems.
} About a year or so ago, we started having problems getting good
coatings...noticable because of excessive charging of coated samples. We
immediately thought of contamination because the target was becoming
discolored after only a couple of uses. If I removed it, cleaned it with
metal polish, etc and reinstalled it, all was well for another couple of
coatings. We tried new pumps, looking for oil leaks etc. but a good vacuum
in the chamber ruled out large leaks and all other signs were negative. We
also changed the argon tank thinking that perhaps this could be a source of
contamination.
} The cryo stage is used in spurts and after a period of inactivity, we
are again gearing up for heavier use. Recently the target was only giving a
good coating on one sample before having to be cleaned....a major job when
you have to warm to room temp from -170oC prior to opening up the chamber. I
finally took the used target over to a Microprobe on campus and did a WDS
analysis on it....turns out that the contamination was aluminum. The housing
that holds the target in place is aluminum. Somehow the aluminum is being
sputtered as well as the gold, producing a poor metal coating and
contaminating the target as well.
} Oxford is trying to get replacement parts for the sputter head but we
are at a loss to figure out why, after all these years, this is happening
and what we can do about it if replacement parts cannot be found.
} By the way, a new crystage runs about $70,000 so we are not
contemplating that move at the moment.
} Debby





From daemon Tue Apr 11 17:10:55 2000



From: gao-at-nirim.go.jp (Gao Yihua)
Date: Tue, 11 Apr 2000 16:37:50 +0900 (JST)
Subject: Who can introduce some classical books

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Dear Colleagues:

Who can introduce some classical books and references about the

interfacial structure between different materials?


Thanks in advance.

Yours sincerely
Gao Yihua




From daemon Tue Apr 11 17:10:55 2000



From: Keith Ryan :      KPR-at-wpo.nerc.ac.uk
Date: Tue, 11 Apr 2000 08:58:05 +0100
Subject: Re: Wrinkles in semi-thins

Contents Retrieved from Microscopy Listserver Archives
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Hildy

Thanks for all the info.

The resin used was a TAAB Laboratories standard Hard pre-mix kit,
mixed and kept in a freezer. Usually no problem but the resin mixture
may have aged.

Infiltration may not have been sufficient, although it was overnight
in the 50/50 mixture acetone/resin (our safety dept. is anti propylene
oxide). We can usually get away with this procedure, but you may have
some good points about this step. I am suddenly back about 20 years!
Our library stopped taking Stain Technol. in the 1980's and I haven't
searched it since!

The wrinkles seem to be most apparent in the area of the cuticle
(which is really not very thick).

So long

Keith Ryan



From daemon Tue Apr 11 17:10:55 2000



From: Van Osta, Peter [JanBe] :      PVOSTA-at-janbe.jnj.com
Date: Tue, 11 Apr 2000 09:58:37 +0200
Subject: Digital imaging concepts in microscopy

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Hi,

I hesitated a bit to post this message as I thought it would be too trivial,
but my personal experience has shown me that people often do not know the
basic concepts needed to acquire good quality images with a digital/video
camera in light microscopy. I want to know if other people working in
digital imaging agree on the very few concepts I think that are essential to
acquire good quality digital images in microscopy.

I simply give an overview of the concepts for quantitative digital
brightfield microscopy:

1) Understand the meaning of Numerical Aperture
2) Use white light, dim with neutral density filters if necessary, do not
turn down the light to "reddish".
3) Set up Koehler illumination !
4) Nyquist sampling (match magnification to CCD-array)
5) Understand the influence of the sampling density on the C.V. of your
measurements
Ian T. Young, Sampling density and quantitative microsocopy Analytical and
Quantitative Cytology and Histology, vol. 10, 1988, pp. 269-275

6) Understand the dynamic range of your image acquisition system (camera +
digitiser)

For a B/W camera:
1) Use a green filter for monochromatic light

For a color camera
1) Use a 3CCD camera, not a single CCD camera
2) Set the white balance

My personal opinion is that if you obey these basic rules you get good
quality images, otherwise you don't. The quality of the images relates
directly to the quality of your analysis and as such to the "quality" of
your conclusions.

Regards,

Peter Van Osta, MD

Senior Scientist Medical Image Analysis
Biological Imaging Laboratory
Life Sciences Department I - 6065
Janssen Research Foundation
Turnhoutseweg 30
B-2340 Beerse
Belgium
Europe

tel.: +32 (0)14 60.60.65 (office hours, GMT+1)
fax.: +32 (0)14 60.57.88

email: pvosta-at-janbe.jnj.com

WWW: http://ourworld.compuserve.com/homepages/pvosta




From daemon Tue Apr 11 17:11:03 2000



From: A.P. Alves de Matos :      apmatos-at-ip.pt
Date: Tue, 11 Apr 2000 11:37:59 +0100
Subject: Tissue Tek machine evaluation

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Dear Collegues

I am in the process of acquiring a Tissue Tek automatic tissue processor,
second hand.
I seem to have a choice between Tissue Tek II or Tissue Tek III, the later
being more expensive of course.
Since I have no experience with such machines, I would like to ask if in
practice there are significant benifits from the more advanced model.
Actually what I want is a reliable way of making lots of paraffin embeddings
with a minimum of sofistication.


Thanks in advance for your answers

Dr. A.P. Alves de Matos
Dental Medical School
Lisbon




From daemon Tue Apr 11 17:11:05 2000



From: Leslie Eibest :      leibest-at-duke.edu
Date: Tue, 11 Apr 2000 08:30:37 -0400
Subject: one more horror story

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A grad student that I had recently trained to use our JEOL
T-20 SEM was trying a little solo work. He had the specimen chamber
door open, turned away from the scope, and looked back just in time
to see one of our monster cockroaches disappearing into the scope.
Rather than call me, or wait for the roach to reappear, he shut the
door and pumped the scope down. He never did confess...



From daemon Tue Apr 11 17:11:09 2000



From: Jo Verbeeck :      joverbee-at-ruca.ua.ac.be
Date: Tue, 11 Apr 2000 16:09:19 +0200 (METDST)
Subject: EELS: need spectrum for comparing

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Thanks Norm for your suggestions

I will be more specific.
I will need to process up to 60 specimens/week in two or three separated
weeks during the year. The rest of the time hand processing is OK. Since the
specimens are prone to dammage upon storage (immunohistochemical techniques
on sponge tissues), I do not want to store them to facilitate processing.
So, it is important to know if the machine can handle this amount of work.
I have one technician that has many other things to do.
Basic assistence should be available from our technical department. Anyhow I
would not trust newer instruments just because they are more recent. I know
of (and have) a few super-new machines (amaizingly expensive) that had to be
thrown to garbage because no one (assistence included) was able to put them
to regular work. It is important to know if the machine model is a well
tested one that usually works for years with no problems or if it has
regular problems.
Since histology and histopathology labs of my knowledge use other tissue
processors, I was not able to get specific advice locally about these
machines.

Thanks
A.P. Alves de Matos

----- Original Message -----
} From: Norm Granholm {granhona-at-email.uc.edu}
To: A.P. Alves de Matos {apmatos-at-ip.pt}
Sent: Tuesday, April 11, 2000 12:57 PM


Hello,

I`m looking for an EELS spectrum containing Oxygen K-edge and some
other edge like Mn or Ti L-edge. Together with low loss spectrum and data
about the angles.
The spectra need to have a power of 2 (1024 for inst.) bins and low loss
and high loss should have same size.
I want to use this for comparison, i`m trying to do some quantitative EELS
work but so far with limited success.
I want to find out wether my program or my data is wrong;)

Thanks,

Jo

*************************************************************
* Jo Verbeeck *
* University of Antwerp *
* Dept. EMAT (Electron Microscopy for Materials Research) *
* e-mail: joverbee-at-ruca.ua.ac.be *
* tel: +32(0)3 218 02 49 *
* fax: +32(0)3 218 02 57 *
*************************************************************



From daemon Tue Apr 11 17:11:15 2000



From: Debby Sherman :      sherman-at-btny.purdue.edu
Date: 11 Apr 2000 09:39:01 -0500
Subject: Re: critical point drying

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------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Try sandwiching the sections between pieces of filter paper. We do this with lots of small samples which we are in danger of loosing. We use large washers as holders for the filter paper sandwiches. The pairs of washers can be secured using small binder clamps (an office product used like paper clips). Or you can knotch the washers so that you can use a piece of wire to hold them together. The knotches keep the wire from slipping off. If you cannot find washers with the correct internal and external dimensions, they are easily made in any machine shop.
I would put the sandwiches together before or during dehydration and then carry the sections through the last ETOH changes as a unit. It takes a bit longer to CPD due to the absorption of the ETOH by the paper but you have a good chance of having flat sections at completion.
Debby

Debby Sherman, Manager Phone: 765-494-6666
Microscopy Center in Agriculture FAX: 765-494-5896
Dept. of Botany & Plant Pathology E-mail: sherman-at-btny.purdue.edu
Purdue University
1057 Whistler Building
West Lafayette, IN 47907-1057

On Monday, April 10, 2000, Dick Briggs {rbriggs-at-Science.Smith.edu} wrote:
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From daemon Tue Apr 11 17:11:17 2000



From: Phoebe J Doss :      pjdoss-at-okstate.edu
Date: Tue, 11 Apr 2000 10:04:57 -0500
Subject: vibration due to truck traffic?

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Microscopist:

Our new lab is supposed to be built next to a four lane highway that has a
lot of 18 wheeler truck traffic. I am concerned about vibration problems
and would like to convince administration that the building should be built
on the other side of the property as far away from the highway as possible.
Any suggestions or known references covering this? Thanks in advance.

Phoebe J. Doss
Manager/Adjunct Instructor
Electron Microscope Lab
Oklahoma State University



From daemon Tue Apr 11 17:11:17 2000



From: ComCryLab1-at-aol.com
Date: Tue, 11 Apr 2000 11:11:04 EDT
Subject: LM-microscope donations needed

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Looking for a few used B & L 7 to 30 power optical
microscopes (or similar- A. O., etc.) to be given to
local vo-tech school. I will pay shipping.
Will trade something if required.
Mike Urbanik
www.crystalguru.com



From daemon Tue Apr 11 17:11:20 2000



From: Scott Whittaker :      sdw-at-biotech.ufl.edu
Date: Tue, 11 Apr 2000 12:25:38 -0400
Subject: Re: vibration due to truck traffic?

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http://www.biotech.ufl.edu/icbr/emcl/db/good_vibrations.html

This is a discussion archived on "Tips & Tricks" from a couple of years ago
dealing with a similar problem. E-mail addresses of the poster and
respondents are included so you might discuss things with them further.

Good luck


At 10:04 AM 4/11/2000 -0500, you wrote:
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} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {
GO GATORS
Scott D. Whittaker 218 Carr Hall
EM Technician Gainesville, FL 32610
University Of Florida ph 352-392-1184
ICBR EM Core Lab fax 352-846-0251
sdw-at-biotech.ufl.edu http://www.biotech.ufl.edu/~emcl/
The home of " Tips & Tricks "







From daemon Tue Apr 11 17:11:21 2000



From: Larry Allard :      l2a-at-ornl.gov
Date: Tue, 11 Apr 2000 12:40:40 -0400
Subject: Re: vibration due to truck traffic?

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Phoebe:

They want to put your lab next to a 4-lane highway...this is a joke, right?

Larry ;-)

PS my suggested reference... 1. Common sense. Vol.1, No. 1, p. 1
another suggestion: make some vibration measurements and check it out...





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Dr. Lawrence F. Allard
Senior Research Staff Member
High Temperature Materials Laboratory
Oak Ridge National Laboratory
1 Bethel Valley Road
Bldg. 4515, MS 6064
PO Box 2008
Oak Ridge, TN 37831-6064

865-574-4981
865-576-5413 Fax
allardlfjr-at-ornl.gov



From daemon Tue Apr 11 17:11:22 2000



From: Nathan Haese :      nathan_haese-at-CompuServe.COM
Date: Tue, 11 Apr 2000 12:50:07 -0400
Subject: vibration due to truck traffic? - Dow Chemical Analytical Lab

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Phoebe,

Several years ago, Dow built a state-of-the-art analytical chemistry
building (including a microscopy lab) in Midland, Michigan. One of the
issues they addressed was nearby truck traffic, just like you have. They
ended up closing the road. Bob Czeislinski (sp?), a member of this
listservice, might be able to help you on some of the tech issues raised to
get the change. (Sorry, I do not have his email address.)

Good luck,

Nathan Haese
Lafayette, CA



From daemon Tue Apr 11 17:11:22 2000



From: HILDEGARD CROWLEY :      hcrowley-at-du.edu
Date: Tue, 11 Apr 2000 10:47:18 -0600 (MDT)
Subject: Re: Wrinkles in semi-thins

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On Tue, 11 Apr 2000, Keith Ryan wrote:

} ------------------------------------------------------------------------
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} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hildy
}
} Thanks for all the info.
}
} The resin used was a TAAB Laboratories standard Hard pre-mix kit,
} mixed and kept in a freezer. Usually no problem but the resin mixture
} may have aged.
}
} Infiltration may not have been sufficient, although it was overnight
} in the 50/50 mixture acetone/resin (our safety dept. is anti propylene
} oxide). We can usually get away with this procedure, but you may have
} some good points about this step. I am suddenly back about 20 years!
} Our library stopped taking Stain Technol. in the 1980's and I haven't
} searched it since!
}
} The wrinkles seem to be most apparent in the area of the cuticle
} (which is really not very thick).
}
} So long
}
} Keith Ryan
}
}
Hi,

One should never leave tissue of any sort in a solution of solvent and
epoxy overnight. It is deleterious to tissue structures and does not
serve to infiltrate.

I do not have a worm. But if I suddenly had one to embed and I had no
experience with worms, I would be very suspicious of it. I would
immediately treat it as though it were really hard to handle. I would do
all the dehydration steps for at least 30 min with a change of ethanol
every 10 minutes. I would open a new bottle of 100% ethanol for the last
three changes. I would use propylene oxide, and evaporate the waste from
a bucket in the hood. I would use PO (dry acetone if you must) and epoxy
in the ratios of 3 to 1 for 1/.2 hour, 2 to one for one hour, 1 to 1 for 2
hours. Then one hour in fresh expoxy. Then new, clean vials, with fresh
epoxy. Perhaps 3 times for 2-3 hours each. Every time a new mixture is
added I would heat with a light bulb directed at the rotator to 37 deg
(not over 40) for about 1.5 hours. Then I would leave it overnight on the
rotator, and start again in the morning. A pain in the neck! But then,
science frequently sucks! In the evening I would embed in fresh material,
and immediately polymerize at 60 deg. NOTE: All steps on rotator! Do
not let material stand in the hood, not even for an hour.

I know nothing of your epoxy kit. I cannot comment on it. Since you have
experience with it, I would not immediately throw it out. Just try
pushing infiltration hard, and then try the vaccum trick. It saved a
whole project for me once.

Good luck,
Hildy



From daemon Tue Apr 11 17:11:23 2000



From: HILDEGARD CROWLEY :      hcrowley-at-du.edu
Date: Tue, 11 Apr 2000 11:01:22 -0600 (MDT)
Subject: Re: Stretch?? Epoxy sections?

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On Thu, 6 Apr 2000, Rosemary White wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear Hildegard,
}
} To follow up a posting a few days ago, what epoxy are you using that does
} not compress during sectioning? I am very curious!
}
} THanks,
}
}
} Rosemary White
} Microscopy Centre
} CSIRO Plant Industry
} GPO Box 1600
} Canberra, ACT 2601
} Australia
}
} phone 61-2-6246 5475
} fax 61-2-6246 5000
} email r.white-at-pi.csiro.au
}
}
}
}
There is no formulation that can be said to not compress during
sectioning. It depends on the way it is handled and the nature of the
tissue being embedded. Please read my postings again which explain in
detail what the basic problems are.

Bye,
Hildy



From daemon Tue Apr 11 17:11:23 2000



From: HILDEGARD CROWLEY :      hcrowley-at-du.edu
Date: Tue, 11 Apr 2000 10:58:40 -0600 (MDT)
Subject: Epoxy formulations

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Hi,

Someone asked me what I use in the laboratory for epoxies, and now I
cannot find the person who asked.

Our general standard is the Luft' medium formulation. It is a requirement
however for reembedding Vibratome or thick sections from glass slides, no
matter what the original section was embedded in. We use Pella Eponate 12
and all other resins come from EMS. (I have no financial holdings in these
companies)

If the end of the project goes only to thick sections, we use
Mollenhauer's Araldite - Epon - DDSA - dibutyl, because this mixture
sections like butter. However, we have to embed keratinized skin so the
embedding procedure is really lengthy.

For immunocytochemistry we try to find out if we have huge quantities of
antigen. We first try one embedment (this is for post-gold) with the
above Araldite 502 mixture. If at first we don't succeed, we give up
immediately! We then go to LR Gold which, in our case, reliably yields
better ultrastructure than the LR White. (and of course, we get much more
label)

All the above formulations are well known and can be found in any good
textbook. Sometimes I construct other formulations (for embedding
chocolate for paperweights for my co-workers) but those instances are
applicable only to what I am about and not of general interest to anyone.
'
Hope this is the answer you wanted.

Bye,
Hildy



From daemon Tue Apr 11 17:11:30 2000



From: Bob Wise :      wise-at-vaxa.cis.uwosh.edu
Date: Wed, 12 Apr 2000 13:57:36 -0500
Subject: Re: Epoxy formulations

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They must taste awful. And aren't they hard to unwrap?

Bob

Hildy wrote:

} Sometimes I construct other formulations (for embedding
} chocolate for paperweights for my co-workers)



From daemon Tue Apr 11 17:11:32 2000



From: Philip Oshel :      peoshel-at-facstaff.wisc.edu
Date: Tue, 11 Apr 2000 14:59:12 -0500
Subject: Re: vibration due to truck traffic?

Contents Retrieved from Microscopy Listserver Archives
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This reminds me of a trick a Cambridge (yes, back then) service
engineer pulled to demonstrate vibration in the SEM room at a former
position. Fill a glass almost full and put it on the floor. Watch the
pretty rings. Do a quick calculation on the height of the waves -- if
they're 1mm high in the glass, at 10,000 times in the SEM they'd be
10 meters high. If any of the admin types have sail boats (or bass
boats, this being Oklahoma), this might make an impression on them.

Phil

} Phoebe:
}
} They want to put your lab next to a 4-lane highway...this is a joke, right?
}
} Larry ;-)
}
} PS my suggested reference... 1. Common sense. Vol.1, No. 1, p. 1
} another suggestion: make some vibration measurements and
} check it out...
}
} } Microscopist:
} }
} } Our new lab is supposed to be built next to a four lane highway that has a
} } lot of 18 wheeler truck traffic. I am concerned about vibration problems
} } and would like to convince administration that the building should be built
} } on the other side of the property as far away from the highway as possible.
} } Any suggestions or known references covering this? Thanks in advance.
} }
} } Phoebe J. Doss
} } Manager/Adjunct Instructor
} } Electron Microscope Lab
} } Oklahoma State University
}
} Dr. Lawrence F. Allard
} Senior Research Staff Member
} High Temperature Materials Laboratory
} Oak Ridge National Laboratory

}}}}}}}}}}}}}}}}}{{{{{{{{{{{{{{{{{
Philip Oshel
Supervisor, AMFSC and BBPIC
Dept. of Animal Health and Biomedical Sciences
University of Wisconsin
1656 Linden Drive
Madison, WI 53706-1581
voice: (608) 263-4162
fax: (608) 262-7420 (dept. fax)



From daemon Tue Apr 11 17:11:39 2000



From: Barbara Foster :      mme-at-map.com
Date: Tue, 11 Apr 2000 17:01:53 -0400
Subject: Re: vibration due to truck traffic?

Contents Retrieved from Microscopy Listserver Archives
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Hi,

I can also tell you first hand about the effect on light microscopy and
microspectrometry equipment. I once had an assignment in a coking facility
which used a switch engine to run coal cars around the yars. Forget about
anything in the higher mag range!

Also, suggest that you talk to any of the EM apps people (Norm Burns, Tim
Maitland?) from the old Cambridge/Leica SEM group (now part of LEO,
Thornwood NY). Cambridge built that facility with a freight train running
through the back yard.

There are companies which will do site evaluations, including some of the
SEM groups. I don't know if there is a charge, but whatever it is, it
would be less expensive than (1) not being able to do research in a brand
new facility (administrators are traditionally very allergic to "egg on the
face" syndrome") or (2) having to move the lab to another location once it
is set up.

Hope this is helpful.

Best regards,
Barbara Foster,President
Microscopy/Microscopy Education
125 Paridon Street Suite 102
Springfield, MA 01118-2130
PH: 413-746-6931 FX: 413-746-9311 email: MME-at-map.com
Website: www.MME-Microscopy.com/education

America's first national consortium of microscopy specialists offering
customized on-site training in all areas of microscopy, image analysis, and
sample preparation
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^



At 10:04 AM 4/11/00 -0500, Phoebe J Doss wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Tue Apr 11 17:11:42 2000



From: White, Woody N :      Woody.N.White-at-mcdermott.com
Date: Tue, 11 Apr 2000 17:34:00 -0500
Subject: Signal Conditioner Source?

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Hello All,

In order to maximize usefulness, I need a dc coupled signal conditioner
between
my WDS analog rate output and the SEM line scan input.

Small and battery powered would be nice... Am open to suggestion, but what
seems to be indicated is an amp with about +- 5 volt of INPUT offset with a
gain
of 1-25 (or more). Output swing should be capable of a minimum of +-5 volts.

(i.e. a couple of op-amps and 2 10- turn pots...)

I can build one, but would rather purchase if I can find one at a reasonable
price.
The MVA from my old ETEC would probably work, but is a NIM bin module and so
would need to be repackaged and powered to stand alone.

Thanks, Woody White
McDermott Technology, Inc.



From daemon Tue Apr 11 17:21:57 2000



From: Arnold, Jim :      jim.arnold6-at-honeywell.com
Date: Tue, 11 Apr 2000 17:12:24 -0500
Subject: Saving Measurements on photos

Contents Retrieved from Microscopy Listserver Archives
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I am currently taking measurement of SEM photos using IPP 4.1 (length
measurements, angles, and thickness)and I am wondering if there is a way to
"burn" these measurement into the photos for saving purposes. Has anyone
done this or am I missing something. Thanks in advance.


Jim Arnold
Senior Quality Technician / Failure Analysis
Honeywell International, Inc.
Aerospace Electronic Systems
Microelectronics and Technology Center
9140 Old Annapolis Rd
Columbia, MD 21045

email: jim.arnold6-at-honeywell.com
voice: (410) 964-4118
fax: (410) 992-5813




From daemon Tue Apr 11 17:21:57 2000



From: Lou Solebello :      microls1297-at-mindspring.com
Date: Tue, 11 Apr 2000 17:15:42 -0500
Subject: Yet one more horror story..........

Contents Retrieved from Microscopy Listserver Archives
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This one is from grad school days. I was doing my thesis on the
petrography and geochemistry of Miocene andesites as part of an
international study on PreHellenic arc volcanism. Since I did all my own
thin section preparations, I became the unpaid "volunteer" to keep the
prep lab in good running condition, and to assist other students. Nothing
compares to being an indentured servant. One day, a coastal sedimentary
graduate student approached me. He was working on temporal barrier
islands formed off the Gulf coast of Florida that formed as a result of
Hurricane Helena in 1986. His major advisor thought it would be good for
him make some impregnated thin sections from core samples, and stain them
for carbonates and feldspars, map the distribution of them. I instructed
him on all the staining procedures including safety
procedures.......emphasizing safety procedured since he would be dealing
with concentrated HF to etch the sections. I decided to stay in the lab to
do some maintenance, and to keep an eye on him. Good thing I did, for
what happened next could have turned into a real sad disaster. The student
knocked over his slide drying rack into the HF bath. Before I could say or
do anything, he immersed both of his hands into the concentrated HF to
save his sections. Fortunately for me, I had been on a volunteer rescue
squad in my teens, and was fully trained for all sorts of accidents. He
was lucky........didnt lose his fingers, but did lose his finger nails,
and his hands were scarred for life. Moral of this story? I should have
done the procedure myself. I took on a potentially grave situation under
my responsibility for a position I was not getting paid for, or properly
insured for by the department. I am glad the fellow didnt suffer worse for
his lack of thought, but I learned a valuable lesson, and held myself
accountable and responsible for what happened. The student didnt..........
Lou Solebello




From daemon Tue Apr 11 19:20:51 2000



From: Mel Dickson :      m.dickson-at-unsw.edu.au
Date: Wed, 12 Apr 2000 09:14:12 +1000
Subject: Re: Critical Point Drying sandwich

Contents Retrieved from Microscopy Listserver Archives
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Has anyone devised some
} sort of holder (sandwich?) that might overcome this problem by
} keeping them flat during their trip through the dryer?
}
} We thank you in advance.
}
} Dick Briggs
} Biology Department
} Smith College
}
}
} you can make a sandwich using a small reusable swinnex filter holder
(holds 13 mm or larger polymer filters to filter liquids being expressed
from a syringe). You saw off the connecting bits leaving the bits which
screw together. Then you can sandwich things between two filters maybe
with a spacer made by including a gasket between the filters. Then you
process the whole assembly. Silver filters from Chuck Garber are good as
they wont dissolve in liquid CO2.....
Dr. Mel Dickson,
Deputy Director, The Electron Microscope Unit,
Adjunct Associate Professor, School of Microbiology & Immunology
The University of New South Wales
UNSW SYDNEY 2052
Australia.
Phone +612 9385 6383 Fax +612 9385 6400



From daemon Tue Apr 11 19:20:52 2000



From: DrJohnRuss-at-aol.com
Date: Tue, 11 Apr 2000 19:27:17 EDT
Subject: Re: Saving Measurements on photos

Contents Retrieved from Microscopy Listserver Archives
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In a message dated 4/11/00 6:44:48 PM, jim.arnold6-at-honeywell.com writes:

} I am currently taking measurement of SEM photos using IPP 4.1 (length
} measurements, angles, and thickness)and I am wondering if there is a way
} to "burn" these measurement into the photos for saving purposes. Has anyone
} done this or am I missing something. Thanks in advance.

With IPP you can label each feature with its measurement value for one
measurement parameter.



From daemon Tue Apr 11 19:20:53 2000



From: Chris Jeffree :      cjeffree-at-srv0.bio.ed.ac.uk
Date: Wed, 12 Apr 2000 00:40:37 +0100
Subject: Turbo pump horror

Contents Retrieved from Microscopy Listserver Archives
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Several years ago, one of my research students was using the
Cambridge S250 SEM on an early solo session. The room was
hushed, in almost in total darkness, and he was giving his total
concentration to the screen while he adjusted the image. As he
moved his hand to the specimen stage controls the turbo-molecular
pump disintegrated without warning, making a crash that sounded
like a metal tray full of spanners being dropped from a great height,
followed immediately by the wailing of alarms. The poor chap was
literally green with shock - he thought he had caused it!

When the column was opened up a glittering cloud of aluminium
alloy powder drifted out. The turbo pump - a double-ended model -
had its rotors and stators intertwined so forcibly that there was no
free play. Presumably it had come to rest from 60,000 rpm in less
than a single rotation. That works out at a damage rate of almost
1billion dollars per second!

Chris


~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Dr Chris Jeffree
University of Edinburgh
Biological Sciences EM Facility
Daniel Rutherford Building
King's Buildings EDINBURGH EH9 3JH
Tel: +44 (0) 131 650 5345
FAX: +44 (0) 131 650 6563

Inveresk Cottage, 26 Carberry Road,
Inveresk, Musselburgh, Midlothian EH21 8PR, UK
Tel. +44 (0) 131 665 6062 / Mobile 0410 585 401
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~



From daemon Tue Apr 11 19:31:31 2000



From: Chris Jeffree :      cjeffree-at-srv0.bio.ed.ac.uk
Date: Tue, 11 Apr 2000 19:24:30 -0500
Subject: pre-final horror story?

Contents Retrieved from Microscopy Listserver Archives
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} } } From: Chris Jeffree {cjeffree-at-srv0.bio.ed.ac.uk}
} } To: microscopy-at-sparc5.microscopy.com
} } Subject: pre-final horror story?
} } Send reply to: c.jeffree-at-ed.ac.uk
} } Date sent: Tue, 11 Apr 2000 16:01:02 +0000
} }
} } Several years ago, one of my research students was using the
} } Cambridge S250 SEM on an early solo session. The room was
} } hushed, in almost in total darkness, and he was giving his total
} } concentration to the screen while he adjusted the image. As he
} } moved his hand to the specimen stage controls the turbo-molecular
} } pump disintegrated without warning, making a crash that sounded
} } like a metal tray full of spanners being dropped from a great height,
} } followed immediately by the wailing of alarms. The poor chap was
} } literally green with shock - he thought he had caused it!
} }
} } When the column was opened up a glittering cloud of aluminium
} } alloy powder drifted out. The turbo pump - a double-ended model -
} } had its rotors and stators intertwined so forcibly that there was no
} } free play. Presumably it had come to rest from 60,000 rpm in less
} } than a single rotation. That works out at a damage rate of almost
} } $1billion per second!
} }
} } Chris
} } ------- End of forwarded message -------
} } =====================================================================
} } DR CHRIS JEFFREE
} } BIOSEM - BIOLOGICAL SCIENCES EM FACILITY
} } UNIVERSITY OF EDINBURGH
} } Daniel Rutherford Building
} } King's Buildings, Mayfield Road
} } EDINBURGH, EH9 3JH, Scotland, UK
} } Tel. #44 131 650 5345
} } FAX. #44 131 650 6563
} } Mobile 0410 585 401
} } email c.jeffree-at-ed.ac.uk
} } SEM / TEM bookings sem-at-ed.ac.uk
} } =====================================================================
}
}
}


~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Dr Chris Jeffree
University of Edinburgh
Biological Sciences EM Facility
Daniel Rutherford Building
King's Buildings EDINBURGH EH9 3JH
Tel: +44 (0) 131 650 5345
FAX: +44 (0) 131 650 6563

Inveresk Cottage, 26 Carberry Road,
Inveresk, Musselburgh, Midlothian EH21 8PR, UK
Tel. +44 (0) 131 665 6062 / Mobile 0410 585 401
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~




From daemon Wed Apr 12 08:02:00 2000



From: Witoon Ngow :      witoon-at-su.ac.th
Date: Wed, 12 Apr 2000 08:59:03 -0700 (GMT)
Subject: unsubscribe

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unsubscribe witoon-at-su.ac.th




From daemon Wed Apr 12 08:02:04 2000



From: jim :      jim-at-proscitech.com.au
Date: Wed, 12 Apr 2000 14:17:47 +1000
Subject: RE: vibration due to truck traffic?

Contents Retrieved from Microscopy Listserver Archives
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Maybe I should have headed this "funny horror stories". I used to run an EM
Unit adjacent to a geology department. One of their more intellectual types
decided that the only place to operate a rock-shaking machine was up against
the outside wall of the darkroom and the SEM rooms. I never bothered to check
the effects on the SEM, but observed that through the enlarger's focus
magnifier the image was dancing. I told the grad student users that the shaker
had to go, complete with reasons. Nothing happened, then I just turned the
shaker off whenever it was on.

Eventually I was confronted by that intellectual quietly asking: "why was I
against his shaker"?
I figured years ago that anger was unprofessional and non-productive, but I
lost it on that occasion. I asked rhetorically, how can you call yourself an
intellectual, when you are unable to work out why a two bob (nickel and dime)
shaker had no place next to an EM Unit. That shaker disappeared on next day.

Maybe I should have sent him an exam to sort out the problem:
My instruments were there first
Put the respective instrument cost into the equation
Consider the difficulty involved in moving his shaker versus rehousing the EM
Unit
Also consider that a heavy shaker is almost designed to produce vibration. A
microscope magnifies and not just objects but vibrations too. So one um of
actually transmitted movement 50000x enlarged is 50mm - I trust that the
movement was less than 1um.

In relation to the highway, it must be noted that you cannot switch that off.
Also its difficult to foretell how much vibration would be transmitted. Its a
question of risk: are those administrators willing to relocate the unit later
or will they find the money to place several instruments on expensive
antivibration devices.
I don't believe its worth the risk; convince them.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

}
}
} Phoebe:
}
} They want to put your lab next to a 4-lane highway...this is a joke, right?
}
} Larry ;-)
}
} PS my suggested reference... 1. Common sense. Vol.1, No. 1, p. 1
} another suggestion: make some vibration measurements and check it
} out...
}
} }
} } Microscopist:
} }
} } Our new lab is supposed to be built next to a four lane highway that has a
} } lot of 18 wheeler truck traffic. I am concerned about vibration problems
} } and would like to convince administration that the building should be built
} } on the other side of the property as far away from the highway as possible.
} } Any suggestions or known references covering this? Thanks in advance.
} }
} } Phoebe J. Doss
} } Manager/Adjunct Instructor
} } Electron Microscope Lab
} } Oklahoma State University
}
} Dr. Lawrence F. Allard
} Senior Research Staff Member
} High Temperature Materials Laboratory
} Oak Ridge National Laboratory
} 1 Bethel Valley Road
} Bldg. 4515, MS 6064
} PO Box 2008
} Oak Ridge, TN 37831-6064
}
} 865-574-4981
} 865-576-5413 Fax
} allardlfjr-at-ornl.gov



From daemon Wed Apr 12 08:02:10 2000



From: Paul Webster :      pwebster-at-hei.org
Date: 12 Apr 00 00:03:17 -0700
Subject: Re: vibration due to truck traffic?

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The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Reply to: Re: vibration due to truck traffic?
Do this trick with a dish of water but reflect a beam of light off the water surface onto a wall and the vibrations are magnified before your eyes.

Paul Webster

Mssage from Phil Oshel:

This reminds me of a trick a Cambridge (yes, back then) service engineer pulled to demonstrate vibration in the SEM room at a former position. Fill a glass almost full and put it on the floor. Watch the pretty rings. Do a quick calculation on the height of the waves -- if they're 1mm high in the glass, at 10,000 times in the SEM they'd be 10 meters high. If any of the admin types have sail boats (or bass boats, this being Oklahoma), this might make an impression on them.

Phil




From daemon Wed Apr 12 08:02:12 2000



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Tue, 11 Apr 2000 21:38:48 -1000 (HST)
Subject: another turbo horror story

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If it ain't broke, don't fix it. This is the lesson I learned the hard
way.

Several years back I was looking something up in the Balzers 400 freeze
fracture manual, and noticed it recommended strongly that the turbo pump
be sent in for reconditioning every 50,000 hours of use (or some such
number). I had no previous experience with turbo pumps, and the
consequences sounded pretty dire, so I was concerned. Since it had run
24/7 for several years, and then off and on for several more, it easily
had whatever number of hours. The facility director was getting ready for
a big project utilizing the instrument and, although he was reluctant, I
convinced him that we should send the turbo pump in before he started.

It came back a few weeks later, and it was clearly not our pump, but
another reconditioned one. As I lifted this large pump out of the box two
small ball bearings bounced onto the floor and rolled away. Still holding
the pump, I watched them go. Then I turned the pump all around in my
arms, looking for any signs of damage or loose parts, but all looked
fine. I considered calling the company, but it was Friday and with the
time difference, it would be days before I got an answer. I figured what
the heck, either it is going to work or not! So I installed it and turned
it on, standing as far away as I could. It started up fine and achieved a
reasonable vacuum, so I left it running over the weekend. Monday
afternoon I decided it was OK, and turned it off.

My first thought was that a jet had crashed into the wall behind me and
that I was going to die. And from the look on the faces of the others in
the lab, they clearly thought they were going to, as well. The horrible
screeching noise actually stops really suddenly as those pumps seize up,
and then the quiet is deafening.

I opened the chamber to find it full of aluminum glitter.

The pump was replaced.

When I talked to some EM service people who had a lot more experience with
turbo pumps, they all seemed to think that one should never overhaul a tp,
but just wait until it crashes. Sure, it's a lot more expensive to repair
it then, but apparently few do fail within the normal lifetime of the
instruments they are on. Sigh.

Aloha,
Tina

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************



From daemon Wed Apr 12 08:02:17 2000



From: Gordon Couger :      gcouger-at-rfdata.net
Date: Wed, 12 Apr 2000 05:42:08 -0500
Subject: Re: Saving Measurements on photos

Contents Retrieved from Microscopy Listserver Archives
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} In a message dated 4/11/00 6:44:48 PM, jim.arnold6-at-honeywell.com writes:
}
} } I am currently taking measurement of SEM photos using IPP 4.1 (length
} } measurements, angles, and thickness)and I am wondering if there is a way
} } to "burn" these measurement into the photos for saving purposes. Has
anyone
} } done this or am I missing something. Thanks in advance.
}
} With IPP you can label each feature with its measurement value for one
} measurement parameter.
}
Years ago I used potassium iodide, calcium chloride and copper sulfate
as a bleach. I don't remember the formula but it is not very critical. You
could use this stuff to mark any silver photographic image. I think the
silver ends up a silver iodide so you would need to refix and wash them.
You would probably want to add a gelling compound to it to keep it from
running starch or wall paper paste would be a good place to start.

A simpler method would be to scratch the film or use India Ink on it.
Magic Marker felt tip pens should work as well. There might be some
bleeding on the emulsion side.

For temporary marking there are opaquing paints to cover pin holes
on litho negatives that is water based and should wash off the base
side just fine. You nearest print shop or graphics art supply can fix
you up with a life time supply for 5 bucks.

Good Luck
Gordon

Gordon Couger gcouger-at-couger.com

Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00




From daemon Wed Apr 12 08:02:18 2000



From: Gordon Couger :      gcouger-at-rfdata.net
Date: Wed, 12 Apr 2000 05:52:27 -0500
Subject: Re: vibration due to truck traffic?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} } } Our new lab is supposed to be built next to a four lane highway that has
a
} } } lot of 18 wheeler truck traffic. I am concerned about vibration
problems
} } } and would like to convince administration that the building should be
built
} } } on the other side of the property as far away from the highway as
possible.
} } } Any suggestions or known references covering this? Thanks in advance.
} } }

I take it you are going in on Hall of Fame. It is not as bad as you
fear but it is bad.

Starting from scratch an air supported floor for the room would not be
too expensive if ou designed it your self. Twenty Five cent pre pound steel
and a decient air pump will handle low frequencie vibratin and active
stuff does a great job from 10HZ up.

Now if you could just move a cross the road to the sheep fram most of
your problems wold dissappear.

There are several minds on campus that have delt with simular situations
and I will be glad to get you togeather.

Good luck
Gordon

Gordon Couger gcouger-at-couger.com

Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00





From daemon Wed Apr 12 08:02:23 2000



From: HARRISm-at-esm-semi.co.uk
Date: Wed, 12 Apr 2000 10:33 +0000 (GMT)
Subject: Subject : SEM + DIGITAL IMAGING

Contents Retrieved from Microscopy Listserver Archives
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A general enquiry regarding integated circuit X section images :

Email . harrism-at-esm-semi.co.uk
Name . Martyn Harris Device Engineering - failure analysis


Question.

In semiconductor failure investigation various etchants and staining
methods are used to obtain and or enhance selected features prior to
FESEM investigation and image capture .
As I now capture images digitally and have the increasing capabilities
of image processing at my fingertips it's possible to ' modify' and
possibly distort the initially accurate image obtained which could
lead to misleading results .

I receive people's images and wonder if they are a result of different
sem capabilities / preparation methods / etches or are they simply
'touched up '. They probably think the same about mine .

Therefore is there any way of controlling image processing to enable
like for like comparison ? or
any international standards ? way of indicating on an image it has
been subject to processing or is it now a case of you cannot believe
what you see ?

Regards.





From daemon Wed Apr 12 08:02:24 2000



From: Andrew Vogt :      andrew.d.vogt-at-abbott.com
Date: Wed, 12 Apr 2000 07:20:23 -0500
Subject: Turbo pump horror

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Was the cause ever determined? Or did I miss it somewhere in the text?

An excellent way to stop a turbo without going through the proper shut down
sequence is to drop something into the turbines. An unwitting young prof at
the grad school I went to inadvertantly dropped I can't remember what in a
turbo pump in the UHV system that he was designing. This was with the pump
running at several hundred thousand rmp. You can imagine the size of this
pump. I am not sure that it stopped in less than one rotation, but it fried
the turbo, non pun intended. He had to replace it more so because he
borrowed the pump from another prof. than because he needed one for his
experiments. You can image how green he was.

The moral of this story is to cover the inlet with a screen. The chances of
anything falling into it is significantly reduced and you can save thousands
of dollars.

Regards to you all,
Andy





cjeffree-at-srv0.bio.ed.ac.uk on 04/11/2000 09:55:57 PM
To: microscopy-at-sparc5.microscopy.com-at-INTERNET
cc:


Several years ago, one of my research students was using the
Cambridge S250 SEM on an early solo session. The room was
hushed, in almost in total darkness, and he was giving his total
concentration to the screen while he adjusted the image. As he
moved his hand to the specimen stage controls the turbo-molecular
pump disintegrated without warning, making a crash that sounded
like a metal tray full of spanners being dropped from a great height,
followed immediately by the wailing of alarms. The poor chap was
literally green with shock - he thought he had caused it!

When the column was opened up a glittering cloud of aluminium
alloy powder drifted out. The turbo pump - a double-ended model -
had its rotors and stators intertwined so forcibly that there was no
free play. Presumably it had come to rest from 60,000 rpm in less
than a single rotation. That works out at a damage rate of almost
1billion dollars per second!

Chris


~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Dr Chris Jeffree
University of Edinburgh
Biological Sciences EM Facility
Daniel Rutherford Building
King's Buildings EDINBURGH EH9 3JH
Tel: +44 (0) 131 650 5345
FAX: +44 (0) 131 650 6563

Inveresk Cottage, 26 Carberry Road,
Inveresk, Musselburgh, Midlothian EH21 8PR, UK
Tel. +44 (0) 131 665 6062 / Mobile 0410 585 401
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~






From daemon Wed Apr 12 08:02:24 2000



From: Frank Shapiro :      franks-at-netcom.ca
Date: Wed, 12 Apr 2000 08:19:15 -0400
Subject: Re: vibration due to truck traffic?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Good trick Paul, but in my checkered past, we used a large (12" diameter) dish filled with mercury. That gave much better reflection of the light and very distinct vibration patterns on the wall.

Cheers,

Frank Shapiro.

Paul Webster wrote:

}
} Do this trick with a dish of water but reflect a beam of light off the water surface onto a wall and the vibrations are magnified before your eyes.
}
} Paul Webster
}
} Mssage from Phil Oshel:
}
} This reminds me of a trick a Cambridge (yes, back then) service engineer pulled to demonstrate vibration in the SEM room at a former position. Fill a glass almost full and put it on the floor. Watch the pretty rings. Do a quick calculation on the height of the waves -- if they're 1mm high in the glass, at 10,000 times in the SEM they'd be 10 meters high. If any of the admin types have sail boats (or bass boats, this being Oklahoma), this might make an impression on them.
}
} Phil






From daemon Wed Apr 12 08:14:39 2000



From: Kristin A Breen :      kristin_a_breen-at-email.mobil.com
Date: Wed, 12 Apr 2000 08:03:00 -0500
Subject: VIbrations - consultant

Contents Retrieved from Microscopy Listserver Archives
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Phoebe,

You may want to take a few minutes and peruse the following site:

http://www.vibeng.com/index.htm

Vibration Engineering Consultants -VEC- has some useful information regarding
site selection, vibration, EM and acoustic interferences, etc.
However, it may help you more to talk with them personally. Craig Franklin
came
to our site in southern New Jersey a few years ago, to survey
potential new locations for our SEM, and made some recommendations, as far as
location, field cancellation and vibration insulation. We ended up
buying the AC/DC field canceling system that he recommended, and a vibration
table. These items have been a huge help in insuring the continued high
resolution capabilities of our SEM. The people at VEC may be able to help make
your case to your management, before the new facility is built.

Kristin Breen
Staff Chemist, Marketing Technical Services Lab
North American Region
ExxonMobil Lubricants and Petroleum Specialties Co.
Paulsboro, NJ (856) 224-2864




From daemon Wed Apr 12 14:43:32 2000



From: anderron-at-us.ibm.com
Date: Wed, 12 Apr 2000 09:31:46 -0400
Subject: Subject : SEM + DIGITAL IMAGING

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



There is a difference between unwittingly taking pictures of artifacts and
fraudulent digital manipulation. In the latter case there have been
several strings on this listserver and elsewhere on digital manipulation,
see the archives. Essentially, digital manipulation of image contrast,
brightness, and gamma to produce a better image is the same as dodging,
burning-in and choosing different contrast grade papers in an old-fashioned
darkroom to enhance but not alter an image, this is OK. Beyond that, it is
all to easy to alter an image digitally. "Alter," in the fraudulent sense.
I don't see anything wrong with deleting specimen preparation
artifacts--scratches, left-over polishing compound, etc.--as long as the
true image content is unaffected. What is and what isn't in this category
is a judgement call on my part. I assume that, unless proven otherwise,
all of my colleagues out there are doing the "right" thing and clearly
stating what manipulations of the image content were performed and why.

With all due respect, Martyn, if I were you I'd worry more about the former
case. SEM imaging of chemically etched integrated circuit cross sections
is horrendously prone to artifact production. We stopped this practice
more than a decade ago, tuning instead to high-angle ion milling for short
time periods. See our paper: Martinek, et al., 1989 MSA Proceedings, p.
720. We've been using GATAN Duo Mill ion millers for this from our TEM
areas. GATAN has brought out a unit specifically to perform this function
for SEM labs. It's the Model 682 (?) PECS or, the new PECS + RIBE system.
(The question mark is mine--I'm not sure of the model number of the simple
PECS system.) Good luck.

Ron

I have no business or financial relationship with GATAN other than being a
long-time satisfied customer.



Ron Anderson, IBM, Hopewell Jct., New York, USA. anderron-at-us.ibm.com

IBM Analytical Services; http://www.chips.ibm.com/services/asg



"HARRISm/-at-esm-semi.co.uk" on 04/12/2000 06:33:00 AM

To: Microscopy-at-sparc5.microscopy.com
cc:



A general enquiry regarding integated circuit X section images :

Email . harrism-at-esm-semi.co.uk
Name . Martyn Harris Device Engineering - failure analysis


Question.

In semiconductor failure investigation various etchants and staining
methods are used to obtain and or enhance selected features prior to
FESEM investigation and image capture .
As I now capture images digitally and have the increasing capabilities
of image processing at my fingertips it's possible to ' modify' and
possibly distort the initially accurate image obtained which could
lead to misleading results .

I receive people's images and wonder if they are a result of different
sem capabilities / preparation methods / etches or are they simply
'touched up '. They probably think the same about mine .

Therefore is there any way of controlling image processing to enable
like for like comparison ? or
any international standards ? way of indicating on an image it has
been subject to processing or is it now a case of you cannot believe
what you see ?

Regards.









From daemon Wed Apr 12 14:43:32 2000



From: Ford M. Royer :      froyer-at-bitstream.net
Date: Wed, 12 Apr 2000 08:42:57 -0500
Subject: Re: Tissue Tek machine evaluation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The TissueTek* brand of histology equipment has been around for many years, and
in my opinion, is at the top of the quality, dependability, and reliability
lists. The confusion here is what exact "TissueTek" models you are
considering. Even using the suffixes "II" and "III" does not completely
identify which TissueTek product you are talking about. It is best to acquire
and use the individual Model numbers to prevent confusion.

What I call a "TissueTek II" processor is the [Model 4640]. This is a basic
"dip and dunk" rotary processor and is considered an "open system" (no fume
control). It is still being manufactured and it can be purchased as a new piece
of equipment. No problem getting spare parts. You would have to provide some
type of fume hood for it. The 4640 is compact enough that it would fit inside a
standard chemical fume hood. If you would only be using it on a random basis
and low volume, this would be a good choice. It can hold up to 120 specimens
per run.

I consider the "TissueTek III" [Model 4660] as the first enclosed tissue
processor with on-board fume control. It is also known as the "V.I.P."
However, this Model was discontinued in 1982-83 and is totally OBSOLETE as far
as spare parts and support from the manufacturer. There are a number of these
units still in service around the world, however.

} From approximately 1983 to 1994 the next generation TissueTek V.I.P.s were the
"K" series with [Model Numbers: 4617, 4618,4619]. They came in three volume
sizes and and could be purchased in either a bench-top or floor configuration.
They still supported by the manufacture, and spare parts are readily available.
These, once again, are "closed systems" with fume control.

The current production models of the "V.I.P." is the "E" series [Models: 4890 &
4894] in the bench top configuration, and [Models 4892 & 4896] in the floor
model configuration. They come in two volume sizes.

Of the above, the only processor that I would not recommend that you consider is
the original TissueTek III VIP [Model 4660].

I hope this answers your questions.

* TissueTek, and TissueTek V.I.P. are registered trademarks of Sakura Finetek,
Inc.

--
Ford M. Royer, MT(ASCP)
Analytical Instruments, Ltd
(Refurbished Histology, Cytology, & General Lab Equipment)
9921 13th Ave. N.
Minneapolis, MN 55441-5004
phone: 800-565-1895, Ext. 17
fax: 612-929-1895
Email: froyer-at-bitstream.net
web site: http://www.aibltd.com

"A.P. Alves de Matos" wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Thanks Norm for your suggestions
}
} I will be more specific.
} I will need to process up to 60 specimens/week in two or three separated
} weeks during the year. The rest of the time hand processing is OK. Since the
} specimens are prone to dammage upon storage (immunohistochemical techniques
} on sponge tissues), I do not want to store them to facilitate processing.
} So, it is important to know if the machine can handle this amount of work.
} I have one technician that has many other things to do.
} Basic assistence should be available from our technical department. Anyhow I
} would not trust newer instruments just because they are more recent. I know
} of (and have) a few super-new machines (amaizingly expensive) that had to be
} thrown to garbage because no one (assistence included) was able to put them
} to regular work. It is important to know if the machine model is a well
} tested one that usually works for years with no problems or if it has
} regular problems.
} Since histology and histopathology labs of my knowledge use other tissue
} processors, I was not able to get specific advice locally about these
} machines.
}
} Thanks
} A.P. Alves de Matos
}
} ----- Original Message -----
} } From: Norm Granholm {granhona-at-email.uc.edu}
} To: A.P. Alves de Matos {apmatos-at-ip.pt}
} Sent: Tuesday, April 11, 2000 12:57 PM
} Subject: Re: Tissue Tek machine evaluation
}
} } Dr. de Matos:
} }
} } I believe some general guidelines are appropriate for you to consider:
} } 1. Get the newest machine you can afford. Older machines will have
} more
} } maintenance requirements and will become obsolete sooner.
} } 2. Is instrument repair readily available? If not then any
} automated
} } system is potentially a problem. Automated systems do break.
} } 4. Your note says "lots of paraffin embeddings". What is "lots"?
} Hand
} } processing is quite feasible for batches of small numbers and if personnel
} help
} } is not limiting (see next). No one likes doing it and an automated system
} lets
} } skilled individuals perform more appropriate tasks. All of this is,
} however, an
} } issue of resource utilization. You have considered these matters already,
} } undoubtedly.
} } 3. If personnel help is a limiting factor, the more automated the
} better
} } for you. If personnel help is not a limiting factor, then the less
} automated the
} } better for you. This may sound strange but I believe it is true. It all
} depends
} } upon who is paying the bills and whether the funds are available for other
} uses.
} } And there are always other uses for funds.
} }
} } Having raised all of those points, I'll tell you that I used a Tek II when
} they
} } first came out ( now some 20 years ago). And this for fewer than a dozen
} samples
} } per week. I wanted skilled individuals to do other tasks than hand dip
} tissues.
} }
} } You might look, also, at Leitz/Leica tissue processors. My experiences
} with
} } Leica are that they provide outstanding equipment at reasonable prices.
} Often
} } they have trade in items available. Were I to have to chose I would head
} this
} } way.
} }
} } Best wishes,
} }
} } Norm
} } (Norman.Granholm-at-uc.edu)
} } Pathology, Univ. Cincinnati
} }
} } Voice 513 558 0182
} } Digital Pager 513 249 3889
} } ===============================



From daemon Wed Apr 12 14:43:33 2000



From: Becky Holdford :      r-holdford-at-ti.com
Date: Wed, 12 Apr 2000 08:47:42 -0500
Subject: Re: vibration due to truck traffic?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


What's funny?Ê My lab is on the 4th floor of a semiconductor fab at the intersection
of two 6-lane freeways.Ê We have 4 SEMs, 3 focused ion beams, and 2 200kV TEMs.Ê We
routinely work over 150KX (SEM) with very few problems.Ê It's a well-designed
building.Ê EMI is more of a problem.

Larry Allard wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} ToÊ Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Phoebe:
}
} They want to put your lab next to a 4-lane highway...this is a joke, right?
}
} LarryÊ ;-)
}
} PSÊ my suggested reference... 1. Common sense. Vol.1, No. 1, p. 1
} ÊÊÊÊÊÊ another suggestion:Ê make some vibration measurements and check it out...
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } ToÊ Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Microscopist:
} }
} } Our new lab is supposed to be built next to a four lane highway that has a
} } lot of 18 wheeler truck traffic.Ê I am concerned about vibration problems
} } and would like to convince administration that the building should be built
} } on the other side of the property as far away from the highway as possible.
} } Any suggestions or known references covering this?Ê Thanks in advance.
} }
} } Phoebe J. Doss
} } Manager/Adjunct Instructor
} } Electron Microscope Lab
} } Oklahoma State University
}
} Dr. Lawrence F. Allard
} Senior Research Staff Member
} High Temperature Materials Laboratory
} Oak Ridge National Laboratory
} 1 Bethel Valley Road
} Bldg. 4515, MS 6064
} PO Box 2008
} Oak Ridge, TN 37831-6064
}
} 865-574-4981
} 865-576-5413Ê Fax
} allardlfjr-at-ornl.gov

--
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Becky HoldfordÊ (r-holdford-at-ti.com)
972-598-1291 (pager)
KFAB Physical Analysis Lab--SEM/FIB
Kilby Center West
Texas Instruments, Inc.
Dallas, TX
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Ê



From daemon Wed Apr 12 14:43:33 2000



From: John Heckman :      heckman-at-pilot.msu.edu
Date: Wed, 12 Apr 2000 09:50:14 -0700
Subject: Phys/Mat: Looking for Bloch Wave Paper

Contents Retrieved from Microscopy Listserver Archives
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Hi There,

I'm trying to track down a paper presented in the early '70s at an EM
meeting in Erice, Italy. The papers were published in a volume called
Electron Microscopy in Materials Science Vol. II, Valdre and Ruedl,
eds. The paper I'd like to get is one by A. Metherell on Bloch waves.
My quest has stymied our interlibrary loan folks here (I did get the 3rd
vol). Anyone got a Vol II or the Metherell paper?

Thanks

John

John Heckman
MSM Department
Michigan State University





From daemon Wed Apr 12 14:43:34 2000



From: Larry Allard :      l2a-at-ornl.gov
Date: Wed, 12 Apr 2000 10:10:38 -0400
Subject: Re: vibration due to truck traffic?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Becky:

I wouldn't expect much vibration either, if I worked in a Billion $
building...Phoebe, what was your budget again?

Larry ;-)




} What's funny? My lab is on the 4th floor of a semiconductor fab at
} the intersection
} of two 6-lane freeways. We have 4 SEMs, 3 focused ion beams, and 2
} 200kV TEMs. We
} routinely work over 150KX (SEM) with very few problems. It's a well-designed
} building. EMI is more of a problem.
}
} Larry Allard wrote:
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} } Phoebe:
} }
} } They want to put your lab next to a 4-lane highway...this is a joke, right?
} }
} } Larry ;-)
} }
} } PS my suggested reference... 1. Common sense. Vol.1, No. 1, p. 1
} } another suggestion: make some vibration measurements and
} check it out...
} }
} } } ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } -----------------------------------------------------------------------.
} } }
} } }
} } } Microscopist:
} } }
} } } Our new lab is supposed to be built next to a four lane highway that has a
} } } lot of 18 wheeler truck traffic. I am concerned about vibration problems
} } } and would like to convince administration that the building
} should be built
} } } on the other side of the property as far away from the highway
} as possible.
} } } Any suggestions or known references covering this? Thanks in advance.
} } }
} } } Phoebe J. Doss
} } } Manager/Adjunct Instructor
} } } Electron Microscope Lab
} } } Oklahoma State University
} }
} } Dr. Lawrence F. Allard
} } Senior Research Staff Member
} } High Temperature Materials Laboratory
} } Oak Ridge National Laboratory
} } 1 Bethel Valley Road
} } Bldg. 4515, MS 6064
} } PO Box 2008
} } Oak Ridge, TN 37831-6064
} }
} } 865-574-4981
} } 865-576-5413 Fax
} } allardlfjr-at-ornl.gov
}
} --
} ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
} Becky Holdford (r-holdford-at-ti.com)
} 972-598-1291 (pager)
} KFAB Physical Analysis Lab--SEM/FIB
} Kilby Center West
} Texas Instruments, Inc.
} Dallas, TX
} ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
}

Dr. Lawrence F. Allard
Senior Research Staff Member
High Temperature Materials Laboratory
Oak Ridge National Laboratory
1 Bethel Valley Road
Bldg. 4515, MS 6064
PO Box 2008
Oak Ridge, TN 37831-6064

865-574-4981
865-576-5413 Fax
allardlfjr-at-ornl.gov



From daemon Wed Apr 12 14:43:34 2000



From: William Tivol :      tivol-at-wadsworth.org
Date: Wed, 12 Apr 2000 10:34:14 -0400
Subject: Re: another turbo horror story

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Tina Carvalho wrote:

} When I talked to some EM service people who had a lot more experience with
} turbo pumps, they all seemed to think that one should never overhaul a tp,
} but just wait until it crashes. Sure, it's a lot more expensive to repair
} it then, but apparently few do fail within the normal lifetime of the
} instruments they are on. Sigh.

Dear Tina,
Yes, turbopumps canibalize themselves spectacularly--they're almost as much

"fun" as high-pressure Hg-vapor lamps. I'd like, however, to put in a good
word
for servicing them on a regular basis. The turbos that were on the HVEM when
I got here were ~200 lbs, turned at ~10,000 rpm, and occasionally ate
themselves.
We soon replaced them with the TPU330 model pumps, which are ~30 lbs., turn
at ~15,000 rpm, and have been humming along at "standby" speed for years. The
vacuum is as good at standby as at full speed, so we have seen no need to go to
the
higher speed. We've changed the oil on a regular schedule and sent the pumps
to
Balzers every two years for bearing changes. There has been no deterioration
in
performance for ~15 years now.
Yours,
Bill



From daemon Wed Apr 12 14:43:35 2000



From: Beverly_E_Maleeff-at-sbphrd.com
Date: Wed, 12 Apr 2000 10:49:05 -0400
Subject: Job opportunity - reposted

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Cell Biologist/Microscopist

SmithKline Beecham, a world class leader in Research and Development, continues
to pioneer innovative pharmaceutical and healthcare products and services. We
have the following opportunity available at our state-of-the-art suburban
Philadelphia facility. Working in our Safety Assessment department you will
provide technical support and scientific input into the design and execution of
studies to elucidate the cellular mechanisms of drug-induced toxicities. You
will prepare biological specimens for transmission and scanning electron
microscopy, confocal microscopy, X-ray microanalysis and immunohistochemistry.
You will also perform qualitative and quantitative data analysis using computer
assisted image analysis systems. We require a BS/MS in a biological science, and
3-5 years of microscopy experience in cell biology, physiology, toxicology.
SmithKline Beecham is dedicated to an innovative workplace and supports you with
career long opportunities and learning. We offer a competitive benefits and
compensation package. For confidential consideration, please forward your
scannable resume to:
SmithKline Beecham
Attention: Human Resources
AD CODE: 2K0325W
c/o National Resume Processing
P.O. Box 1070
Burlington, MA 01803 USA

Indicating ad code is essential. Principals only, no agencies, please. For a
full listing of current opportunities, or to submit a resume online, visit our
website at www.sb.com/careers.

**********************************************************************************************

Please respond directly to the address in the advertisement, and not to me.

Regards,
Bev Maleeff




From daemon Wed Apr 12 14:43:37 2000



From: George Langford, Sc.D. :      amenex-at-amenex.com
Date: Wed, 12 Apr 2000 11:08:59 -0400
Subject: Re: vibration due to truck traffic?

Contents Retrieved from Microscopy Listserver Archives
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Hallo magnetic Microscopists !

Everyone's been discussing vibration in this thread
(talk about slavish adherence to topic ...) but there's
another consideration - magnetic disturbances. Those
truck have steel frames, so they'll disturb the Earth's
magnetic field as they pass by. I inadvertently made a
magnetometer once, and I could see the inflence of
vehicles passing by fifty feet away on my oscilloscope
screen. At M.I.T. there was a lot of concern about the
nearby elevator in one EM lab ...

Best regards,
George Langford, Sc.D., who's got no vested interest in
Earth's magnetic field except when out hiking.
amenex-at-amenex.com



From daemon Wed Apr 12 14:43:42 2000



From: David Henriks :      Henriks-at-CompuServe.COM
Date: Wed, 12 Apr 2000 13:13:51 -0400
Subject: SEM + DIGITAL IMAGING

Contents Retrieved from Microscopy Listserver Archives
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Dear Martyn:

Several years ago (MSA in Cleveland if I recall correctly), there was some
sort of roundtable discussing the ethics of image manipulation. I can't
remember much more than that, but perhaps those with a better memory who
may have participated in the discussion would have some good input.

Best regards-

David
Writing at 9:53:20 AM on 04/12/2000

***************************************************************************
************************

David Henriks
Vice President TEL: 800-728-2233
(toll free in the USA)
South Bay Technology, Inc. +1-949-492-2600
1120 Via Callejon FAX: +1-949-492-1499
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

***************************************************************************
************************

} } } } } Please visit us at http://www.southbaytech.com { { { { {

Manufacturers of precision sample preparation equipment and supplies for
metallography, crystallography and electron microscopy.
Message text written by
INTERNET:"HARRISm-at-esm-semi.co.uk"-at-sparc5.microscopy.com
} A general enquiry regarding integated circuit X section images :

Email . harrism-at-esm-semi.co.uk
Name . Martyn Harris Device Engineering - failure analysis


Question.

In semiconductor failure investigation various etchants and staining
methods are used to obtain and or enhance selected features prior to
FESEM investigation and image capture .
As I now capture images digitally and have the increasing capabilities

of image processing at my fingertips it's possible to ' modify' and
possibly distort the initially accurate image obtained which could
lead to misleading results .

I receive people's images and wonder if they are a result of different

sem capabilities / preparation methods / etches or are they simply
'touched up '. They probably think the same about mine .

Therefore is there any way of controlling image processing to enable
like for like comparison ? or
any international standards ? way of indicating on an image it has
been subject to processing or is it now a case of you cannot believe
what you see ?

Regards.

{



From daemon Wed Apr 12 14:43:49 2000



From: Mitch McCartney :      Mitch.McCartney-at-alconlabs.com
Date: Wed, 12 Apr 2000 14:31:40 -0500
Subject: Position

Contents Retrieved from Microscopy Listserver Archives
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Scientist
Retina Research - Degenerative Disease

Company: Alcon Laboratories, Inc., a wholly owned subsidiary of Nestle,
S.A., has 10,000 employees and worldwide sales of $2.4 billion. It is the
global leader in the discovery, development, manufacture and marketing of
ophthalmic pharmaceuticals and medical devices. Alcon expects to double its
sales over the next five years and has achieved a profit growth rate of
approximately 11% over the last several years. Historically, the company
commits 10% of sales to research and development. Products developed in the
last ten years generate 50% of current sales. The current product pipeline
is strong. Alcon was recently renamed to the Fortune List of the 100 Best
Companies to Work for in America.

As the successful candidate, you will conduct research in the area of
retinal degeneration aimed at identifying novel chemical agents to prevent
neurodegenerative disease using histopathologic and cell culture techniques.
You will participate in the design and provide expertise in preparation and
histopathologic evaluation of plastic and paraffin embedded retinal tissue,
including immunocytochemical techniques. You will also provide technical
expertise in cell/tissue based retina models; participate in handling,
manipulating, and dosing of various laboratory species; and interpret,
summarize, and communicate experimental results in appropriate formats.

Qualified candidates will have 1) at least nine years of applicable
experience, probably in a hospital or university laboratory, following
receipt of a B.S. in biology or a related discipline, or 2) at least three
years of applicable experience followed by receipt of an M.S. with thesis in
biology or a related discipline. Demonstrated competency in histology
techniques is required. Experience in cell/tissue culture is a plus.

Alcon professionals enjoy state-of-the-art facilities in a year-round
business casual environment. Our company offers competitive salaries and a
wide array of excellent benefits: a very generous retirement plan and
dollar-for-dollar matching (up to 5%) 401K, medical, dental, vision, life,
and accident insurance, death, dismemberment, illness and disability
benefits, tuition reimbursement, employee credit union, adoption assistance,
dependent care, and wellness programs, on-site fitness center, running
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An Equal Opportunity and Affirmative Action Employer. Pre-employment drug
testing.

Please forward your salary requirements and your resume to:

Job64_1261-at-careers.alconlabs.com
Reference Code: SRR





From daemon Wed Apr 12 18:42:40 2000



From: =shAf= :      mshaf-at-darkwing.uoregon.edu
Date: Wed, 12 Apr 2000 13:39:07 -0700
Subject: RE: Subject : SEM + DIGITAL IMAGING

Contents Retrieved from Microscopy Listserver Archives
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Martyn Harris writes ...

} ...
} Question.
}
} In semiconductor failure investigation various
} etchants and staining
} methods are used to obtain and or enhance selected
} features prior to
} FESEM investigation and image capture .
} As I now capture images digitally and have the
} increasing capabilities
} of image processing at my fingertips it's possible to
} ' modify' and
} possibly distort the initially accurate image obtained
} which could
} lead to misleading results .
}
} I receive people's images and wonder if they are a
} result of different
} sem capabilities / preparation methods / etches or are
} they simply
} 'touched up '. They probably think the same about mine .
}
} ...

Surely you are not implying similar distortions were never a
possibility in the wet darkroom??

It is possible to put (for example) a fine checkerboard pattern in a
small box which would imply this image has never been subjected to
"blur", "sharpening", or many other kernal operations ... or you could
install a standard grayscale to imply brightness, contrast and gamma
are original ... BUT, you would need trust the author didn't install
the markers after distorting.

=shAf= :o)



From daemon Wed Apr 12 18:42:40 2000



From: Dusevich, Vladimir :      DusevichV-at-umkc.edu
Date: Wed, 12 Apr 2000 15:45:53 -0500
Subject: RE: Subject : SEM + DIGITAL IMAGING

Contents Retrieved from Microscopy Listserver Archives
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It's an old problem. A darkroom is a good tool
for image manipulation too - just less handy and
needs more experience.

Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
3127 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy

} -----Original Message-----
} From: "HARRISm-at-esm-semi.co.uk"-at-sparc5.microscopy.com
} [mailto:"HARRISm-at-esm-semi.co.uk"-at-sparc5.microscopy.com]
} Sent: Wednesday, April 12, 2000 5:33 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Subject : SEM + DIGITAL IMAGING
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListser} ver/FAQ.html
}
}
} --------------------------------------------------------------
} ---------.
}
}
}
} A general enquiry regarding integated circuit X section images :
}
} Email . harrism-at-esm-semi.co.uk
} Name . Martyn Harris Device Engineering - failure analysis
}
}
} Question.
}
} In semiconductor failure investigation various etchants
} and staining
} methods are used to obtain and or enhance selected
} features prior to
} FESEM investigation and image capture .
} As I now capture images digitally and have the
} increasing capabilities
} of image processing at my fingertips it's possible to '
} modify' and
} possibly distort the initially accurate image obtained
} which could
} lead to misleading results .
}
} I receive people's images and wonder if they are a
} result of different
} sem capabilities / preparation methods / etches or are
} they simply
} 'touched up '. They probably think the same about mine .
}
} Therefore is there any way of controlling image
} processing to enable
} like for like comparison ? or
} any international standards ? way of indicating on an
} image it has
} been subject to processing or is it now a case of you
} cannot believe
} what you see ?
}
} Regards.
}
}
}
}



From daemon Wed Apr 12 18:42:43 2000



From: Debby Sherman :      sherman-at-btny.purdue.edu
Date: 12 Apr 2000 16:47:51 -0500
Subject: Last call for parts

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We have disconnected our old Philips EM-200 and will send it to salvage within the next week. If anyone wants parts from it or the others we have in storage (used for parts) please contact me immediately. All used parts (other than vacuum tubes) are free for the asking but you will need to pay shipping.
We also have a Reichert OMU-3 ultramicrotome which can be used for parts. It is not functional at the moment.
Debby

Debby Sherman, Manager Phone: 765-494-6666
Microscopy Center in Agriculture FAX: 765-494-5896
Dept. of Botany & Plant Pathology E-mail: sherman-at-btny.purdue.edu
Purdue University
1057 Whistler Building
West Lafayette, IN 47907-1057



From daemon Wed Apr 12 18:42:46 2000



From: Paul Webster :      pwebster-at-mailhouse.hei.org
Date: 12 Apr 00 15:45:21 -0700
Subject: FEBS Practical Course

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Course Announcement:

FEBS Stereology and Immunocytochemistry course to be held at the University of Oslo in Norway. For details see: http://www.hei.org/htm/curs.htm

This is an intensive practical course covering all aspects of specimen preparation for immunocytochemistry. The practical part of the course is supported by in-depth lectures explaining preparation protocols and their theoretical background.

Subjects covered include stereology (quantitation), chemical fixation, rapid freezing, freeze substitution, cryoultramicrotomy, specimen contrasting, antibody labeling, pre-embedding labeling, antibody and colloidal gold preparation, and specimen evaluation.

Participants are encouraged to bring their own samples.

Due to the intensive practical nature of this course places are limited, so apply early.
Paul Webster, Ph.D
House Ear Institute
2100 West Third Street
Los Angeles, CA 90057
phone:213 273 8026
fax: 213 413 6739
e-mail: pwebster-at-hei.org
http://www.hei.org/htm/aemi.htm



From daemon Wed Apr 12 18:42:47 2000



From: Paul Webster :      pwebster-at-mailhouse.hei.org
Date: 12 Apr 00 16:11:27 -0700
Subject: vibration due to traffic

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Having gone through a similar problem with vibrations, I can fully appreciate what might happen. However, I cannot give you any formal information for you to support your cause, I can only offer you my story.

I moved to my insitute to set up an imaging laboratory but before I arrived, a vibration survey had been performed on the site chosen for the EM's. This site passed inspection.

When I arrived, I looked at the site and suggested it might not be suitable. I was instantly presented with the very professional survey file which said the site was suitable for electron microscopes, (as were about 10 other locations throughout the building).

When the EM supplier came in to survey the site, their standards were very different to the generic vibration expert, and the site failed (not surprizing considering it was in the middle of the 4th floor!). Every other site we looked at failed too, so we were stuck with attempting to make things work using an anti-vibration platform under the first microscope we installed (a TEM). Amazingly, the supplier of the anti-vibration platform did not even come to look at the site when I contacted them. They seemed to have no intention of doing a site survey, and were not even interested in obtaining information from the microscope supplier about the sort of problem we were attempting to correct.

We put the TEM on the very expensive air table, which made it 8' taller, and thus more difficult to operate, and tested it. The platform did not help at all in isolating the low frequency vibration that seemed to be running through the building. It seems that if the vibrating frequency is low enough, even the anti-vibration platform moves! It was also very difficult to operate a machine you couldn't touch when taking a picture.
Eventually we found a stable site, renovated it and installed the microscopes there. All is now in order and the microscopes are performing to specification. Interestingly, the space is next to an in-door parking lot but the flow of traffic, although noticable to people in the lab, does not affect the microscopes. These are mostly slow moving cars with a few SUV's and pick-up trucks shaking the ground occassionally. My guess is that this is because the microscopes are installed between closely spaced support pillars on the side of the building. (Can't wait for the big earthquake to shake them up a bit!)

My advice would be to explain just how sensitive the EM's are to vibration, especially the low frequency shaking you can get from moving trucks and trains, and that the laboratory be located in as quiet a place as possible. Make sure that any vibration surveys are carried out by EM specialists, not by local heros, and keep away from floating platforms.

I am willing to re-tell ALL my experiences with sorting out our problem to anyone who wants or needs to listen. Correcting our mistakes was VERY costly.

Regards,

Paul Webster, Ph.D
House Ear Institute
2100 West Third Street
Los Angeles, CA 90057
phone:213 273 8026
fax: 213 413 6739
e-mail: pwebster-at-hei.org
http://www.hei.org/htm/aemi.htm



From daemon Thu Apr 13 00:38:25 2000



From: JoAnn Buchanan :      redhair-at-leland.Stanford.EDU
Date: Wed, 12 Apr 2000 18:48:39 -0500
Subject: trucks aren't the only problems

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I thought about adding this story to the horror list but now it
seems even
more appropriate for the truck vibration problem. I quote directly from the
book "Principles and Practise of Electron Microscope Operation" by Agar and
Chescoe: The main sources of trouble, which can directly affect the
resolution obtained with the instrument, are mechanical vibrations and
electric fields.
Several years ago we set up an EM facility in the basement of our new,
state of the art, 5 story building here at Stanford. The engineers
surveyed the room and proclaimed it fine for EM use. After using the
microscope for a few weeks I noticed that I could never get a negative that
was in perfect focus. I collected negatives from several other users and
they all had the same problem. We contacted the engineers and one of them
spent the entire day trying to get perfect image of fresnel fringes. By
the time they turned of all the fans in our building (boy, did it get hot
and stinky), he was able to focus. It turned out a giant electrical cable
ran directly underneath the room to power the building ventilation. It
was, of course, not in use yet when they surveyed the new building! Not to
mention the nearby elevators that were not in use during survey time
either. Sadly for us, rather than spend $10,000.00 on an H frame to
eliminate the problem, they moved the old scope out and gave the space to a
molecular biology lab instead. Here we are 7 years later without a decent
EM facility in our building.




From daemon Thu Apr 13 00:38:29 2000



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Wed, 12 Apr 2000 18:17:38 -0700
Subject: TP service

Contents Retrieved from Microscopy Listserver Archives
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Dear Bill,

The service is important, no question about that. But I agree, also, with
Tina, that it is not bad practice "do not disturb" working equipment. For
instance, our TP on JEM1200-EX TEM is original pump coming with instrument
15+ years ago. It was never serviced at all. Our JEOL service engineer
claimed that it is oldest working original TP on the JEM1200-EX series in
US. From time to time they called me asking does TP still working? And it
is work. I don't remember the exact model of TP. This is BALZERS for
sure. I think that electronics life depends in many cases not from how
long it operates but how many times you shut it ON and OFF. This kind of
"law" works perfectly for major electronic components as well as for CRTs,
computer components (HDs for instance). I guess, it may works for TPs too.
During start/stop TP components may sense some stress: more stress,
shorter life (we all know about that). I am experimented with magnetically
levitated TP from SEIKO (no financial interest) now. It works great. It
uses bearings only at low speed, at high speed the rotor is levitated.
Combination of this TP and "scroll pump" (oil free) gives me absolutely
oil-free vacuum system. I'll tell readers of this ListServer what happens
with that TP later. I am not going to service it at all.

Sergey


} Date: Wed, 12 Apr 2000 10:34:14 -0400
} From: William Tivol {tivol-at-wadsworth.org}
} Subject: Re: another turbo horror story
} Sender: tivol-at-wadsworth.org
} To: microscopy-at-sparc5.microscopy.com
} X-Mailer: Mozilla 4.07C-SGI [en] (X11; I; IRIX64 6.5 IP21)
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu
http://www.bol.ucla.edu/~sryazant




From daemon Thu Apr 13 00:38:40 2000



From: earlw-at-pacbell.net
Date: Wed, 12 Apr 2000 19:55:51 -0700
Subject: subscribe

Contents Retrieved from Microscopy Listserver Archives
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I haven't been receiving the listserver emails for about one week.
Please re-subscribe.

Earl Weltmer



From daemon Thu Apr 13 00:38:50 2000



From: COFAB2-at-aol.com
Date: Thu, 13 Apr 2000 00:38:40 EDT
Subject: good ideas

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


As a new associate of Don G. of Microscopy Today I am interested located
interesting techniques and Problem/Solution type of information on all types
of microscopy.



From daemon Thu Apr 13 08:00:47 2000



From: Victor Sidorenko :      antron-at-space.ru
Date: Thu, 13 Apr 2000 13:53:48 +1000
Subject: Re: TP service

Contents Retrieved from Microscopy Listserver Archives
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Dear Sergey!

In my dictionary I have found expression relevant to the situation: to
go from one extreme to another.
By my opinion to keep without service the mechanism rotated with
60,000 rpm is other extreme.
By analogy it is possible to refuse to change oil in car motors...?
Regards.

Victor Sidorenko, ANTRON Co.Ltd., scientific service, Moscow, Russia.



} ---------------------------------------------------------------------
---
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
America

} Sergey Ryazantsev Ph. D.
} Electron Microscopy
} UCLA School of Medicine
} Department of Biological Chemistry
} Box 951737
} Los Angeles, CA 90095-1737
}
} Phone: (310) 825-1144
} Pager: (310) 845-0248
} FAX (departmental): (310) 206-5272
} mailto:sryazant-at-ucla.edu
} http://www.bol.ucla.edu/~sryazant
}
}
}



From daemon Thu Apr 13 17:30:10 2000



From: Joel McClintock :      jmcclin-at-pop.uky.edu
Date: Thu, 13 Apr 2000 21:50:29 -0400
Subject: Coolwell Manuals & info

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} From: Hall, Ernest L (CRD)
Sent: Thursday, April 13, 2000 7:48 AM
To: 'MSA Listserver Dist'


Please correct me if I am wrong, but I think I learned from this list that
Coolwell is defunct? I have a model SE-075W CZ that I am having trouble
with. No schematics and/or manuals to be found. Does anyone know where I
might find these or is willing copy important info from manuals they have on
this model? I would appreciate it.

Still in the fact finding phase but I suspect the thermostat is the main
trouble with this unit. Do any of you know a supplier for these? Thank you
in advance.

Joel McClintock
U of Kentucky



From daemon Thu Apr 13 17:30:16 2000



From: Gib Ahlstrand :      giba-at-puccini.cdl.umn.edu
Date: Thu, 13 Apr 2000 09:41:50 -0500 (CDT)
Subject: Re: good ideas

Contents Retrieved from Microscopy Listserver Archives
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Responding to the message of {c5.40b6c0e.2626a950-at-aol.com}
from "COFAB2-at-aol.com"-at-sparc5.microscopy.com:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} As a new associate of Don G. of Microscopy Today I am interested located
} interesting techniques and Problem/Solution type of information on all types
} of microscopy.
}
}


Whooooooooo are you.......who-oo....oo-oo. } :o

(With appologies to Pete Townsend)


Gib



Gib Ahlstrand
Electron Optical Facility, University of Minnesota, Dept. Plant Pathology
495 Borlaug Hall, St. Paul, MN. USA. 55108 (612)625-8249
612-625-9728 FAX, giba-at-puccini.cdl.umn.edu
http://biosci.umn.edu/MIC/consortium.html



From daemon Thu Apr 13 17:30:18 2000



From: Bill Chissoe :      wchiss-at-ou.edu
Date: Thu, 13 Apr 2000 09:34:59 -0600
Subject: SEM bacterial protocol

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I have a student that wants to look at Actinomycete colonies in the SEM.
I have not looked at bacterial COLONIES before. How would one prepare
such a sample?

TIA,
Bill Chissoe

--
=============================================================
Bill Chissoe III
Electron Microscopist
University of Oklahoma
770 Van Vleet Oval
Norman, Ok. 73019
E-mail: wchiss-at-ou.edu Ph. (405)325-4391
=============================================================




From daemon Thu Apr 13 17:30:19 2000



From: Mitch McCartney :      Mitch.McCartney-at-alconlabs.com
Date: Thu, 13 Apr 2000 10:57:38 -0500
Subject: Location of Position: Ft. Worth

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My apologies to everyone for not thoroughly reading the
advertisement. I posted the message for our Human Resources Department. I
didn't realize that Ft. Worth wasn't mentioned. Accordingly, to clarify
the advertisement, the position would be at our R&D facility in Fort Worth,
Texas, U.S.A.

Mitch
-------------------
Mitchell D. McCartney, Ph.D.
Associate Director
Central Sciences

Scientist
Retina Research - Degenerative Disease

Company: Alcon Laboratories, Inc., a wholly owned subsidiary of Nestle,
S.A., has 10,000 employees and worldwide sales of $2.4 billion. It is the
global leader in the discovery, development, manufacture and marketing of
ophthalmic pharmaceuticals and medical devices. Alcon expects to double its
sales over the next five years and has achieved a profit growth rate of
approximately 11% over the last several years. Historically, the company
commits 10% of sales to research and development. Products developed in the
last ten years generate 50% of current sales. The current product pipeline
is strong. Alcon was recently renamed to the Fortune List of the 100 Best
Companies to Work for in America.

As the successful candidate, you will conduct research in the area of
retinal degeneration aimed at identifying novel chemical agents to prevent
neurodegenerative disease using histopathologic and cell culture techniques.
You will participate in the design and provide expertise in preparation and
histopathologic evaluation of plastic and paraffin embedded retinal tissue,
including immunocytochemical techniques. You will also provide technical
expertise in cell/tissue based retina models; participate in handling,
manipulating, and dosing of various laboratory species; and interpret,
summarize, and communicate experimental results in appropriate formats.

Qualified candidates will have 1) at least nine years of applicable
experience, probably in a hospital or university laboratory, following
receipt of a B.S. in biology or a related discipline, or 2) at least three
years of applicable experience followed by receipt of an M.S. with thesis in
biology or a related discipline. Demonstrated competency in histology
techniques is required. Experience in cell/tissue culture is a plus.

Alcon professionals enjoy state-of-the-art facilities in a year-round
business casual environment. Our company offers competitive salaries and a
wide array of excellent benefits: a very generous retirement plan and
dollar-for-dollar matching (up to 5%) 401K, medical, dental, vision, life,
and accident insurance, death, dismemberment, illness and disability
benefits, tuition reimbursement, employee credit union, adoption assistance,
dependent care, and wellness programs, on-site fitness center, running
track, cafeteria, and company store, innovative paid time off and holidays,
and retiree medical coverage.

An Equal Opportunity and Affirmative Action Employer. Pre-employment drug
testing.

Please forward your salary requirements and your resume to:

Job64_1261-at-careers.alconlabs.com
Reference Code: SRR





From daemon Thu Apr 13 17:30:19 2000



From: Laura Rhoads :      laura-at-lsrhoads.com
Date: Thu, 13 Apr 2000 12:00:18 -0400
Subject: vibration due to Mother Ship

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What about when the Mother Ship passes overhead?

Seriously, George has brought up an interesting point as I myself have
experienced the magnetic-interference-caused-by-powerline problem. The best
thing to do in all these cases is have the vendor (in my case John D from
JEOL) show up with the Earthquake Meter and Magnetic Treasure Finder and do
a survey before the decision-makers decide anything. It's even better if
you can work with the vendor BEFORE you let "them" plan anything.

This is one situation where asking forgiveness instead of permission
doesn't bode well...

"They" are watching...

Laura

}
} Hallo magnetic Microscopists !
}
} Everyone's been discussing vibration in this thread
} (talk about slavish adherence to topic ...) but there's
} another consideration - magnetic disturbances. Those
} truck have steel frames, so they'll disturb the Earth's
} magnetic field as they pass by. I inadvertently made a
} magnetometer once, and I could see the inflence of
} vehicles passing by fifty feet away on my oscilloscope
} screen. At M.I.T. there was a lot of concern about the
} nearby elevator in one EM lab ...
}
} Best regards,
} George Langford, Sc.D., who's got no vested interest in
} Earth's magnetic field except when out hiking.
} amenex-at-amenex.com
}



From daemon Thu Apr 13 17:30:21 2000



From: JIM ROMANOW :      bsgphy3-at-uconnvm.uconn.edu
Date: Thu, 13 Apr 2000 11:31:11 -0500
Subject: Coolwell Manuals & info

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Joel,

Here is all I know about Coolwell:

Coolwell Co. (Out of Business 4/99)
26 Law Drive, Fairfield, NJ 07004
973 882-6611, 800 367-5665
Bought out by: Lytron, Inc. (They have schematics)
55 Dragon Court, Woburn, MA 01801
Application engineer, Greg Ducharm, 781 933-7300
East coast sales, Scott Martin
Project engineer, Lonnie Fultz,4/00, Chillers
Independent- Frank Haze 800 367-5665
(SEE PECO MANUFACTURING FOR TEMP CONTROL)

PECO Manufacturing Co., Inc.
Portland, OR
Sunn(?) Division
503 233-6401
Tempurature control for Coolwell chillers
# TC103 025 C not available
OEM only. Made for Coolwell in lots of 100 only
(Coolwell list price was $170 each 1/99)

Switch used on temp control was made by: C & K (Unimax)
Original part #WHB152-9-W
C & K new part # HBS2KCB4SP011C (available from Newark Electronics #07F041)
22A, 125,277VAC, 15A 480VAC
1/4HP 125VAC, 1/2HP 250 270VAC

I have 3 Coolwell chillers (vintage 1993 and 1996), have replaced the
temperature control on 2 of them in the past year and am out of spare
controls. My units were over heating and shutting down. The temp control is
no longer available (unless all of us Coolwell owners pitch in and buy 100
of them). [I suspect that the sensing bulb is ok but that the electrical
switch has worn out. Test the bulb by immersing in hot water then cold
water while watching for expansion and contraction near the switch
actuator.] The switch could be replaced by drilling out it's securing
rivots and replacing (part # above). Use appropriate screws to replace
rivots as the switch must not slip. I have just ordered my new switches and
will be testing this fix soon.

Please let me know what you find. Coolwells were used on many SEMs so I
think we should have a few others interested in this problem. I have
schematics for our SE series units with various options and Lytron has been
very helpful.
Good Luck.
Jim


---------------------------

On 4/13/00 Joel McClintock wrote:

Please correct me if I am wrong, but I think I learned from this list that
Coolwell is defunct? I have a model SE-075W CZ that I am having trouble
with. No schematics and/or manuals to be found. Does anyone know where I
might find these or is willing copy important info from manuals they have on
this model? I would appreciate it.

Still in the fact finding phase but I suspect the thermostat is the main
trouble with this unit. Do any of you know a supplier for these? Thank you
in advance.

Joel McClintock
U of Kentucky

James S. Romanow
The University of Connecticut
Physiology and Neurobiology Department
Electron Microscopy Facility
U-2131
Storrs, CT 06269-2131
bsgphy3-at-uconnvm.uconn.edu
860 486-2914 voice
860 486-1936 fax




From daemon Thu Apr 13 17:30:27 2000



From: Wil Bigelow :      bigelow-at-engin.umich.edu
Date: Thu, 13 Apr 2000 14:02:30 -0400
Subject: RE: Inexpensive Binoc OMs

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As I recall, a few days ago someone asked about a source of an inexpensive
abinocular optical microscope. Just yesterday I received an advertisment
from the Cole Parmer Co. (800-323-4340; www.coleparmer.com) for a 20X
binocular microscope for a base price of $159 (Cat. No.PP-03904-01), $220
when equipped with a top illuminator (PP-03904-02), and $240 equipped with
both top and bottom illuminators (PP-03904-03).

Wilbur C. Bigelow, Prof. Emeritus
Materials Sci. & Engr., University of Michigan
Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu;
Fx:734-763-4788; Ph:734-662-5237




From daemon Thu Apr 13 17:30:29 2000



From: Wil Bigelow :      bigelow-at-engin.umich.edu
Date: Thu, 13 Apr 2000 15:57:07 -0400
Subject: RE: Tina's vac problem

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Tina:

It is possible that the reading you are getting from your vacuum gauge is
not as good as you expect because the gauge itself became contaminated with
oil. I presume the high vacuum gauge on your instrument is a Penning
gauge, as is the case for most EMs these days. If so, it might be useful
to take it off the instrument and clean it. We just had an incident where
a Penning gauge got so badly fouled that it would not fire at all, and thus
prevented the vacuum system from switching over to the high vacuum
evacuation mode, and so the vacuum could never become good enough to allow
the high voltage to be turned on.

It is usually not too difficult to clean a Penning gauge. The general
construction of these gauges is discussed in Sect. 3.2.2, p. 99, of my book
'Vacuum Methods in Electron Microscopy" and illustrated in Fig. 3.12 on
p. 101. Cleaning involves getting rid of the carbonaceous deposit that
forms on the inside wall of the gauge tube, and on the insulator that
supports the anode ring. Usually, depending on the design, these gauges
can be disassembled, somewhat as shown in Fig. 3.12, whereupon various
methods can be used to remove the carbonaceous deposits. One approach, of
course, is to use some kind of an abrasive (try Revere Ware Stainless Steel
Cleaner, or even a fine grade of metallographic polishing paper. Don't use
steel wool, because pieces of it will get attracted by the strong magnet
and become very troublesome to remove). The Hitachi service engineer was
successful in cleaning our gauge by boiling the gauge tube (after removing
the magnet from around it) and anode ring assembly in a STRONG detergent
solution (try Alconox, or a similar Lab. detergent) for 30 to 60 minutes,
periodically scrubbing with a toothbrush,

Good luck!

Wilbur C. Bigelow, Prof. Emeritus
Materials Sci. & Engr., University of Michigan
Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu;
Fx:734-763-4788; Ph:734-662-5237




From daemon Thu Apr 13 17:30:30 2000



From: Bob Wise :      wise-at-vaxa.cis.uwosh.edu
Date: Fri, 14 Apr 2000 16:24:31 -0500
Subject: Re: Coolwell Manuals & info

Contents Retrieved from Microscopy Listserver Archives
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on 4/13, Jim Romanov wrote:

} Please let me know what you find. Coolwells were used on many SEMs so I
} think we should have a few others interested in this problem. I have
} schematics for our SE series units with various options and Lytron has been
} very helpful.


We replaced the original thermostat on our Coolwell SE unit with an "ETC
Single Stage Electronic Temperature Controller" and a "1309007-044 ETC
Temperature Sensor" from Ranco (8115 U.S. 42 N., Plain City, Ohio, 43064).
The unit bolts right to the front of the Coolwell chiller and works just
fine. It works over a tempertature range of -30F to +220F and a
differential of 1F to 30F. I seem to recall that the whole shebang was
less than $100 and our campus refrigerator guy installed it with no
problems. I can fax the spec sheets to anyone who is interested. I
believe I got the idea from Maggy Piranian through this list.

Bob
Dr. Robert R. Wise
Associate Professor of Plant Physiology
Department of Biology
University of Wisconsin Oshkosh
Oshkosh, WI 54901
(920) 424-3404
wise-at-uwosh.edu
http://www.uwosh.edu/departments/biology/wise/wise.html



From daemon Thu Apr 13 18:36:18 2000



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Thu, 13 Apr 2000 16:21:13 -0700
Subject: Re: Coolwell Manuals & info

Contents Retrieved from Microscopy Listserver Archives
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Dear Joel,
I have had good luck getting a local HVAC (heating, ventilation and air
conditioning) service firm to service my cooling water recirculators, which
are Haskris. They have repaired all of them without problems. They may be
able to fit replacement parts without having to resort to the manufacturer.
At 09:50 PM 4/13/00 -0400, you wrote:
}
} Please correct me if I am wrong, but I think I learned from this list that
} Coolwell is defunct? I have a model SE-075W CZ that I am having trouble
} with. No schematics and/or manuals to be found. Does anyone know where I
} might find these or is willing copy important info from manuals they have on
} this model? I would appreciate it.
}
} Still in the fact finding phase but I suspect the thermostat is the main
} trouble with this unit. Do any of you know a supplier for these? Thank you
} in advance.
}
} Joel McClintock
} U of Kentucky

Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Thu Apr 13 21:32:31 2000



From: Praveena Bhaskara :      bubbyp-at-hotmail.com
Date: Thu, 13 Apr 2000 18:33:22 -0500
Subject: Help with Forced Modulation Techniques

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Hi,
I was wondering whether anyone out there can help me out on the forced
modulation and phase contrast technoques. I'm familiar with the basics of
the AFM ,ie., contact and the non-contact modes. Now I want to learn the
advances methods. Can anyone suggest any good books that give the full
techniques?
Praveena

Praveena M Bhaskara
MS Student
Chemical Engineering Department
University of Massachusetts, Lowell
Lowell, MA 01854
Email:bubbyp-at-hotmail.com
PH:978-459-0175
______________________________________________________
Get Your Private, Free Email at http://www.hotmail.com




From daemon Thu Apr 13 21:32:31 2000



From: deweese-at-fas.harvard.edu () (by way of Nestor J. Zaluzec)
Date: Thu, 13 Apr 2000 18:37:29 -0500
Subject: Re: ? mitochondria. With 400 times magnification

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Email: deweese-at-fas.harvard.edu
Name: Alex de Weese
School: Harvard College
Question: Hello,
I just finished a lab where we did cell fractionation, and looked at
nuclear and cytosolic fractions stained with toluidine blue under a zeiss
compound light microscope. I was wondering why I couldn't see
mitochondria. With 400 times magnification, I expected to be able to see
them in the cytosolic fraction. Is it that the mitochondria are not
staining? Is it a problem with contrast?
Thanks for your help.
Sincerely,
Alex de Weese


---------------------------------------------------------------------------




From daemon Thu Apr 13 21:32:32 2000



From: Paul Webster :      pwebster-at-mailhouse.hei.org
Date: 13 Apr 00 17:53:44 -0700
Subject: Re: good ideas

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Reply to: Re: good ideas
Well put.

Messages with only a name, or even less, I just delete now.

It is so easy to sign these things, and so interesting to know who is writing them.

Regards,

Paul Webster

Paul Webster, Ph.D
House Ear Institute
2100 West Third Street
Los Angeles, CA 90057
phone:213 273 8026
fax: 213 413 6739
e-mail: pwebster-at-hei.org
http://www.hei.org/htm/aemi.htm


Gib Ahlstrand wrote:
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} Responding to the message of {c5.40b6c0e.2626a950-at-aol.com}
} from "COFAB2-at-aol.com"-at-sparc5.microscopy.com:
} } } } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
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} } -----------------------------------------------------------------------.
} } } } } } As a new associate of Don G. of Microscopy Today I am interested located } } interesting techniques and Problem/Solution type of information on all types } } of microscopy.
} } } } }
}
} Whooooooooo are you.......who-oo....oo-oo. } :o
}
} (With appologies to Pete Townsend)
}
}
} Gib
}
}
}
} Gib Ahlstrand
} Electron Optical Facility, University of Minnesota, Dept. Plant Pathology
} 495 Borlaug Hall, St. Paul, MN. USA. 55108 (612)625-8249
} 612-625-9728 FAX, giba-at-puccini.cdl.umn.edu
} http://biosci.umn.edu/MIC/consortium.html
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}
Paul Webster, Ph.D
House Ear Institute
2100 West Third Street
Los Angeles, CA 90057
phone:213 273 8026
fax: 213 413 6739
e-mail: pwebster-at-hei.org
http://www.hei.org/htm/aemi.htm



From daemon Thu Apr 13 21:32:33 2000



From: Paul Webster :      pwebster-at-mailhouse.hei.org
Date: 13 Apr 00 17:59:48 -0700
Subject: RE: Coolwell Manuals & info

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Reply to: RE: Coolwell Manuals & info
Yes Joel,

Frank Haze, who owned Coolwell (and what magnificent chillers they are) sold the company to Litron (phone: 781 933 7300). I do not know to what extent they will continue to support the chillers but the contact I was given at Litron was Lohny Fultz.

Our chillers are currently being maintained by our refrigeration service contractors and they appear to be doing a great job of it (Cascade Refrigeration, Irvine CA).

Regards,

Paul Webster

Paul Webster, Ph.D
House Ear Institute
2100 West Third Street
Los Angeles, CA 90057
phone:213 273 8026
fax: 213 413 6739
e-mail: pwebster-at-hei.org
http://www.hei.org/htm/aemi.htm


Joel McClintock wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
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} -----------------------------------------------------------------------.
}
}
} Please correct me if I am wrong, but I think I learned from this list that
} Coolwell is defunct? I have a model SE-075W CZ that I am having trouble
} with. No schematics and/or manuals to be found. Does anyone know where I
} might find these or is willing copy important info from manuals they have on
} this model? I would appreciate it. }
} Still in the fact finding phase but I suspect the thermostat is the main
} trouble with this unit. Do any of you know a supplier for these? Thank you
} in advance. }
} Joel McClintock
} U of Kentucky
}
}
}
}
} RFC822 header
} -----------------------------------
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From daemon Fri Apr 14 07:24:34 2000



From: Oldrich Benada :      benada-at-biomed.cas.cz
Date: Fri, 14 Apr 2000 08:58:21 +0200
Subject: Durst condenser lenses - cleaning

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Hello,
We have in our lab a Durst Laborator 138S enlarger. After all those
years of it's perfect working, there are some layers of dust on
condenser lenses. Does anybody know, how to safely clean the surface
of condenser lens? Thanking you in advance.
O. Benada

+-----------------------------------+
Oldrich Benada
Acad. Sci. CR
Institute of Microbiology
Laboratory of electron microscopy
Videnska 1083
CZ - 142 20 Prague 4 - Krc
Czech Republic
+------------------------------------+
Phone: +420-2-4752399
Fax: +420-2-4715743



From daemon Fri Apr 14 18:25:30 2000



From: ComCryLab1-at-aol.com
Date: Fri, 14 Apr 2000 09:20:02 EDT
Subject: LM: Inexpensive Binoc OMs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Prof. Bigelow,
Thanks for that info. I'll check it out.
Mike Urbanik
www.crystalguru.com



From daemon Fri Apr 14 18:25:31 2000



From: Stephen McCartney :      stmccart-at-vt.edu
Date: Fri, 14 Apr 2000 09:34:42 -0400
Subject: Help with Forced Modulation Techniques

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Praveena: One place to start is Digital Instruments web site--DI.com.
They have many applications notes on the subjects you are interested in. I
believe other manufacturers also have helpful web sites. Steve



Hi,I was wondering whether anyone out there can help me out on the forced
modulation and phase contrast technoques. I'm familiar with the basics of
the AFM ,ie., contact and the non-contact modes. Now I want to learn the
advances methods. Can anyone suggest any good books that give the full
techniques?
Praveena





Stephen McCartney
Research Associate
Virginia Tech
Materials Institute
2108 Hahn Hall
Blacksburg, VA 24061-0344
USA

TEL: 540-231-9765
FAX: 540-231-8517



From daemon Fri Apr 14 18:25:31 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 14 Apr 2000 07:46:12 -0700
Subject: Re: Durst condenser lenses - cleaning

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


If it like most condenser enlargers, the condenser lenses are
opposing each other and retained in a metal cylinder. Just
remove the cylinder, lenses and clean them with a cloth towel,
moistened in a solution of Simple Green or a store-bought
lens cleaning solution. rinse the lenses, wipe them dry and
use a duster to get all moisture and lint off of them. That ought
to do it. Check your enlarger's lens while you are at it.
And also see if the bellow and lens plate areas need blowing out.
Lots of dust collects in those nooks and crannies.

gary g.


At 11:58 PM 4/13/00 , you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Fri Apr 14 18:25:32 2000



From: Chris Walker :      chris.walker-at-physics.org
Date: Fri, 14 Apr 2000 15:37:47 +0100
Subject: EM - Magnetic field strength in SEMs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Can anyone tell me the typical magnetic field strength at the sample in an
SEM and (if possible) how fast the field drops off with distance from the
axis?. Any references would be gratefully received.

Regards
Chris Walker



From daemon Fri Apr 14 18:25:32 2000



From: William Tivol :      tivol-at-wadsworth.org
Date: Fri, 14 Apr 2000 10:59:20 -0400
Subject: Re: TP service

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Sergey Ryazantsev wrote:

Dear Sergey,


} I think that electronics life depends in many cases not from how
} long it operates but how many times you shut it ON and OFF.

I agree that this is true, especially with computers and, in this case,
pump controllers. BTW, we have had to fix the controllers on several
occasions.


} This kind of
} "law" works perfectly for major electronic components as well as for CRTs,
} computer components (HDs for instance). I guess, it may works for TPs too.

Maybe not. There are two competing factors: There is wear on the bear-
ings due to the inevitable friction of running. Changes due to acceleration
of the pump during shutdown and startup cause an increase in wear. To get
optimal performance, these factors must be balanced. Our pumps have
mechanical bearings, which will wear during the normal operation of the
pump, so we have chosen to get the bearings replaced every two years.
Since we are replacing the part that wears, the pump lifetime should not
be shortened by this procedure. We also change the bearing oil on a regular
schedule; I think that this prevents any problems from oil breakdown and/or
acidification. There is negligable bearing wear produced by the oil changes,
and the bearing replacement will prevent the accumulation of even this wear.

}
} During start/stop TP components may sense some stress: more stress,
} shorter life (we all know about that). I am experimented with magnetically
} levitated TP from SEIKO (no financial interest) now. It works great. It
} uses bearings only at low speed, at high speed the rotor is levitated.

With this kind of pump stop/start cycles will cause the mechanical
bearings to be used, so there is logic in leaving them running continually.


}
} Combination of this TP and "scroll pump" (oil free) gives me absolutely
} oil-free vacuum system. I'll tell readers of this ListServer what happens
} with that TP later. I am not going to service it at all.

Sounds like a good system; I'm glad you plan to post how things go.
Yours,
Bill Tivol



From daemon Fri Apr 14 18:25:33 2000



From: HOWARD L. MULHERN :      mulhern-at-hub.tch.harvard.edu
Date: Fri, 14 Apr 2000 11:50:54 -0400
Subject: TEM RESOLUTION

Contents Retrieved from Microscopy Listserver Archives
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I have a question concerning TEM Resolution.

We are currently in the process of replacing our aging Phillips
300 TEM with a brand new all digital instrument that is going to be
installed in virtually the same position as the old scope. As
supervisor of the surgical pathology EM facility I have to say we have
never had to question the resolution of the 300 and given the often sub
optimal specimens that we work with the photo's are crisp and sharp. Any
out of focus photographs are usually the result of out of focus
operators, the occasional helicopter landing on the roof and yes trucks.
This is seldom an issue and certainly not the scope.
Here's our problem. We just had a site visit to measure the
electrical and magnetic forces in the room (standard for installation of
any new microscope). And to my suprise the room has an
electrical/magnetic problem that "May effect the resolution of the new
instrument" and quite possibility "the new scope will not meet spec in
this location". So what to do. I seriously doubt that the old scope is
giving me better resolution than the new scope will and I can't change
locations at this point.
So for biology or really anyone embedding in epoxy resins is
there a standard or a number in angstroms that we can say is the minimum
we will accept. If I am pleased with my photo's taken at 60,000 X do
really need to insist that the instrument be capable of taking a sharp
photo at 300,000X.

Howard Mulhern



From daemon Fri Apr 14 18:25:34 2000



From: Michael Bode :      mb-at-Soft-Imaging.com
Date: Fri, 14 Apr 2000 10:11:31 -0600
Subject: SEM + DIGITAL IMAGING

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


David, Martyn,

this problem is not limited to image processing, of course. IP only
makes it easier to use or abuse these things. If you think about how
you get the signals from the SEM (or any other image source), there are
many ways the signal can be distorted. For example, the detector may not
be linear (as is the case for many old cameras and, for that matter, the
human eye), signals may be distorted during transmission, noise is
added, the film for photographing has certain characteristics, and the
darkroom work can be more of an art than science.
Forensic science has had to deal with this for some time. If an argument
in a court of law can be made that a picture has been "doctored", it
will lose it's effectiveness or be dismissed. In Forensics it is very
important to keep a record of the whereabouts and the processing done to
"evidence" at all times. Sometimes people burn a "gray scale" into the
image before they do any processing. This gray scale will then show what
happened to the image during processing. Of course, this is not
foolproof as anybody can put on another gray scale after processing. But
this is the same as inventing measurement data, which can be done, but
is not a real problem in science as it just cannot be repeated
independently.
If you need to be absolutely sure, get a step-by-step recipe of the
image processing and have someone else repeat it. If the results are
different, you have reason to be concerned. If they are identical, you
have your answer.
You need to be familiar with the possibilities and shortfalls of image
processing. You can start with an image that shows just noise, apply
some Fourier-filters and other processing and end up with a periodic
structure on the image. This, however, is not due to the image
processing, it is due to using the wrong tools (or use the tools the
wrong way).

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: David Henriks [mailto:Henriks-at-CompuServe.COM]
Sent: Wednesday, April 12, 2000 11:14 AM
To: Martyn Harris; Micro Listserver



Dear Martyn:

Several years ago (MSA in Cleveland if I recall correctly), there was
some
sort of roundtable discussing the ethics of image manipulation. I can't
remember much more than that, but perhaps those with a better memory who
may have participated in the discussion would have some good input.

Best regards-

David
Writing at 9:53:20 AM on 04/12/2000

************************************************************************
***
************************

David Henriks
Vice President TEL: 800-728-2233
(toll free in the USA)
South Bay Technology, Inc. +1-949-492-2600
1120 Via Callejon FAX:
+1-949-492-1499
San Clemente, CA 92673 USA e-mail:
henriks-at-southbaytech.com

************************************************************************
***
************************

} } } } } Please visit us at http://www.southbaytech.com { { { { {

Manufacturers of precision sample preparation equipment and supplies for
metallography, crystallography and electron microscopy.
Message text written by
INTERNET:"HARRISm-at-esm-semi.co.uk"-at-sparc5.microscopy.com
} A general enquiry regarding integated circuit X section images :

Email . harrism-at-esm-semi.co.uk
Name . Martyn Harris Device Engineering - failure analysis


Question.

In semiconductor failure investigation various etchants and
staining
methods are used to obtain and or enhance selected features prior
to
FESEM investigation and image capture .
As I now capture images digitally and have the increasing
capabilities

of image processing at my fingertips it's possible to ' modify' and

possibly distort the initially accurate image obtained which could
lead to misleading results .

I receive people's images and wonder if they are a result of
different

sem capabilities / preparation methods / etches or are they simply

'touched up '. They probably think the same about mine .

Therefore is there any way of controlling image processing to
enable
like for like comparison ? or
any international standards ? way of indicating on an image it has
been subject to processing or is it now a case of you cannot
believe
what you see ?

Regards.

{



From daemon Fri Apr 14 18:25:34 2000



From: lherault :      lherault-at-bu.edu
Date: Fri, 14 Apr 2000 00:34:39 -0400
Subject: polyacrylamide gel for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


A student has come to me with the following problem. Can anyone assist us?

Ron L.

Please cc answers to:

saeeda-at-bu.edu


Does anybody know of any good fixing/dehydrating techniques for viewing this
sample using the SEM? Alcohol and CPD dehydration techniques are not
yielding good results and there is a lot of distortion of the gel. I am
looking for something that will enable me to preserve the features of the
gels, and analyze their morphology. These gels will be patterned with
collagen and cells will be grown on them. I am interested in viewing these
patterns of collagen, and the corresponding patterns of cell growth.
Thanks.

Saeeda



From daemon Fri Apr 14 18:25:35 2000



From: George Laing :      scisales-at-ngscorp.com
Date: Fri, 14 Apr 2000 13:09:45 -0400
Subject: Durst condenser lenses - cleaning

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


If the lens is coated, which some Durst condensers are, I would suggest
starting with
a Blower Brush, available in most camera shops. Hopefully you can
blow/brush most or all
the dust away. If that fails, one of the new "microfiber" lens cloths work
very well
on most lenses. A high quality photographic lens cleaning solution should
be safe,
but I would try to clean without any chemicals first.

George Laing
National Graphic Supply
Albany, NY USA
www.ngscorp.com


-----Original Message-----
} From: Oldrich Benada [mailto:benada-at-biomed.cas.cz]
Sent: Friday, April 14, 2000 2:58 AM
To: Microscopy-at-sparc5.microscopy.com


Hello,
We have in our lab a Durst Laborator 138S enlarger. After all those
years of it's perfect working, there are some layers of dust on
condenser lenses. Does anybody know, how to safely clean the surface
of condenser lens? Thanking you in advance.
O. Benada

+-----------------------------------+
Oldrich Benada
Acad. Sci. CR
Institute of Microbiology
Laboratory of electron microscopy
Videnska 1083
CZ - 142 20 Prague 4 - Krc
Czech Republic
+------------------------------------+
Phone: +420-2-4752399
Fax: +420-2-4715743




From daemon Fri Apr 14 18:25:35 2000



From: Norman Michaud :      Norman_Michaud-at-meei.harvard.edu
Date: Fri, 14 Apr 2000 13:43:14 -0400
Subject: TEM- used microtomes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does anyone know of a supplier of used ultramicrotomes? I'm looking for a
Leica ultracut E, in good working order. Is there anyone who is dismantling
a TEM lab who wants to sell one?
Thanks.

Norman Michaud
Director, Morphology
Mass Eye and Ear Infirmary
Ophthalmology-5th flr.
243 Charles St, Boston, MA 02114
norman_michaud-at-MEEI.Harvard.edu
Tel:617-573-3316; Fax:617-573-4290



From daemon Fri Apr 14 18:25:36 2000



From: Philip Oshel :      peoshel-at-facstaff.wisc.edu
Date: Fri, 14 Apr 2000 13:51:15 -0500
Subject: Re: polyacrylamide gel for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I can suggest two possibilities:1) atomic force microscopy of the
hydrated gel; 2) high-pressure freezing of the gel, followed by
either cryoSEM or cryo-ultramicrotomy and cryoTEM (cryoSEM would be
simpler than cryo-ultramicrotomy).

Phil

} A student has come to me with the following problem. Can anyone assist us?
}
} Ron L.
}
} Please cc answers to:
}
} saeeda-at-bu.edu
}
}
} Does anybody know of any good fixing/dehydrating techniques for viewing this
} sample using the SEM? Alcohol and CPD dehydration techniques are not
} yielding good results and there is a lot of distortion of the gel. I am
} looking for something that will enable me to preserve the features of the
} gels, and analyze their morphology. These gels will be patterned with
} collagen and cells will be grown on them. I am interested in viewing these
} patterns of collagen, and the corresponding patterns of cell growth.
} Thanks.
}
} Saeeda

--
}}}}}}}}}}}}}}}}}{{{{{{{{{{{{{{{{{
Philip Oshel
Supervisor, AMFSC and BBPIC
Dept. of Animal Health and Biomedical Sciences
University of Wisconsin
1656 Linden Drive
Madison, WI 53706-1581
voice: (608) 263-4162
fax: (608) 262-7420 (dept. fax)



From daemon Fri Apr 14 18:25:38 2000



From: Susanne S¿rensen :      sus.sus-at-danbbs.dk
Date: Fri, 14 Apr 2000 21:52:34 +0200
Subject: TEM "Cryosections, lipid"

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello world
I think i have tried everything, but still!!
Is there somebody, who nows how to detect Glycolipid/lipid on cryo ultrathin sections?
And yes, i have read Dr. Wim's article, where he compare different metodes.
I have been on a course in Austria, where they also told me, that this ( Dr. Wim) would be the way to decet lipid.
But in the real world, it does not work. The lipid is floating all over the sections.
Tanks for your help
Sincerely
Sus S¿rensen



From daemon Fri Apr 14 19:02:58 2000



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Fri, 14 Apr 2000 16:07:08 -0700
Subject: Re: TP service

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Bill,
It was nice discussion there. Thanks for your nice reply. I totally agree
with you. No question, the good service in time is a great deal!
Unfortunately the service sometimes is not such great as you have with
yours TPs. Again, sometimes people just do not have enough money to do
service in time.

As for magnetically levitated TP, you right, it is good idea to keep it
running continuously. Currently, I am working on my system in the way to
be able to insert samples through air-lock, than it will be possible do not
break vacuum to load samples. This is my plan. Wish me luck.
Sergey

} Date: Fri, 14 Apr 2000 10:59:20 -0400
} From: William Tivol {tivol-at-wadsworth.org}
} Subject: Re: TP service
} Sender: tivol-at-wadsworth.org
} To: microscopy-at-sparc5.microscopy.com
} X-Mailer: Mozilla 4.07C-SGI [en] (X11; I; IRIX64 6.5 IP21)
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu
http://www.bol.ucla.edu/~sryazant




From daemon Fri Apr 14 19:03:01 2000



From: Caroline Schooley :      schooley-at-mcn.org
Date: Fri, 14 Apr 2000 15:34:34 -0700
Subject: Re: LM: Inexpensive Binoc OMs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Mike -

There are a LOT of suppliers of inexpensive light microscopes in the
educational marketplace; many of the scopes are remarkably good. You'll
find a list of suppliers on the Project MICRO web page (URL below). Get
several catalogs; if you compare descriptions and photos, you'll realize
that prices can vary almost 50% on the same item.

Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/PCI/pci.html




From daemon Fri Apr 14 21:17:47 2000



From: catchanangel-at-aol.com
Date: Fri, 14 Apr 2000 21:10:58 -0500
Subject: Question: photobleaching vs quenching

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Colleagues

Can anyone explain this to Angela. It's not my field.
She is not on the Listserver.

Nestor
Your Friendly Neighborhood SysOp


Email: catchanangel-at-aol.com
Name: Angela Lowry
School: Deer Valley High School

Question: What is the difference between photobleaching and quenching in
two-photon laser scanning microscopy?

---------------------------------------------------------------------------




From daemon Fri Apr 14 21:49:45 2000



From: Lou Solebello :      microls1297-at-mindspring.com
Date: Friday, April 14, 2000 3:11 PM
Subject: Re: TP service

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I agree with Bill Tivol. It is less stressfull on electrical circuits and
contacts to leave the power on. To do so prolongs the life of the part, as
well as the reliability of voltage flow through an instrument. A good
example is XRD and XRF tubes. Rule #1 I learned as a grad student was to
always leave the power on, keep a current flowing through the tubes. Why?
A power current "burns in" at a single point on the tube. If the power is
shut off, the burn in point can drift. Drift actually weakens the tube,
which is the opposite of what you would expect. The drift will also cause
current fluctuations, creating another source of error reducing the
precision and accuracy of the measurements. At $3000-$4000 per pop on a
tube, it is prudent to do what ever you can to extend its life. Same applies
to any electrical instrumentation or accessories.

Lou Solebello
-----Original Message-----
} From: William Tivol {tivol-at-wadsworth.org}
To: microscopy-at-sparc5.microscopy.com {microscopy-at-sparc5.microscopy.com}




From daemon Fri Apr 14 22:51:25 2000



From: Steve Chapman :      protrain-at-emcourses.com
Date: Sat, 15 Apr 2000 20:31:36 +0100
Subject: Re: TEM RESOLUTION

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



----- Original Message -----
} From: Michele Palmer {moonlite-at-csd.uwm.edu}
To: {wchiss-at-ou.edu}
Sent: Friday, April 14, 2000 7:53 PM


Hi

The question of much resolution do we really need and what resolution is
obtainable often confronts the EM service engineer and the EM consultant?

As mentioned before we at Protrain buy instruments for clients or in
conjunction with clients. On many occasions the proposed site does not meet
the manufacturers requirements in their entirety but circumstances mean we
must go ahead.

Whilst these situations are not ideal one must consider the clients
application and the desired performance for that application. On many
occasions checking an instrument in a clients laboratory, where the client
does not see a problem in their results (5,000 to 30,000X), we see image
instability at 200,000X. As a service engineer you judge the problem and
decide if it is a simple fix or a cause for deep investigation. Often
discussions with the client result in keeping going whilst constantly
checking the magnitude of the fault. This route may be followed for many
years before the engineer, as the client still has no visible micrograph
problems, decides to get in and chase the fault.

So what am I saying? In the 70s high quality work was carried out on TEM
that had a point to point resolution of around 1nm. We are all aware that
1nm at 100,000X is equal to 0.1mm on the micrograph and we need a hand lens
to visualise this! In most routine medical observations, other than virus
work, the typical max is about 30,000X, about 3nm limited by
the section thickness (?) so would it not be sufficient to use a 1nm
machine?

Many biologists still use very low kV, i.e. 80, so they are degrading their
0.3nm instrument still further. Running at 100 or 120kV will provide better
quality images
using dark room procedures to aid contrast. Higher accelerating voltage
will help
with fields and the modern antivibration systems, available as discussed
earlier this month, will help overcome floor vibration.

So my advice after working with TEM for 36 years all over the world in all
sorts of very poor environments is GO FOR IT! I will not take up space by
relating the amazing stories of dreadful sites and superb microscopy, it
certainly does happen!

Good luck

Steve Chapman
Senior Consultant
Protrain - for consultancy and training world wide
Tel & Fax +44 01280 814 774
e-mail protrain-at-emcourses.com
web www.emcourses.com




From daemon Sun Apr 16 16:44:45 2000



From: Steve Beck :      becks-at-sunynassau.edu
Date: Sun, 16 Apr 2000 12:51:15 -0400
Subject: Summer 2000 - TEM Course Announcement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


SUMMER I 2000 COURSE ANNOUNCEMENT - Transmission Electron Microscopy
(BIO. 221-Section B)

NASSAU COMMUNITY COLLEGE, Garden City, Long Island, New York

A five week, Summer Session I 2000 semester, course in Biological
Transmission Electron Microscopy is being offered by the Biology Department
of Nassau Community College. This is a 4 credit course offered four days
per week (Monday through Thursday) between the hours of 8:00 am and NOON.
Classes will begin on May 22 and end on June 22, 2000.

This is a "hands-on" course emphasizing biological specimen preparation,
ultra-thin sectioning involving block trimming, glass knifemaking and
operation of the ultramicrotomes (Sorvall MT-2B and LKB Ultrotome III),
thick and ultra-thin section mounting and contrast staining (UA and Pb
citrate), grid support films (formvar, carbon), student operation of the
TEM (Hitachi HS-8, Philips EM 300) and production of electron micrographs
through the process of black & white photography, and electron micrograph
analysis. Students will work on a chosen sample(s) with the goal of
producing a portfolio of high quality TEM photomicrographs of that
sample(s).

The course is widely transferrable and the cost per credit is reasonable at
$86 per credit (for Nassau County residents or New York State residents
with a certificate of residency).

More information about the Bio-Imaging Center at NCC, course descriptions
and syllabi, and the beginnings of a student gallery of EM
photomicrographs is available at our web site. The URL is
{http://www.sunynassau.edu/webpages/biology/becks.htm} .

Interested individuals should register as soon as possible since the course
is limited to a total enrollment of ten (10) students.

If you have further questions, you should e-mail me directly at the address
below.

For information about mail or telephone registration (Dial-a-Course) point
your browser to http://www.sunynassau.edu/courses/Sum00/index.html.

Stephen J. Beck
Associate Professor
Bio-Imaging Center/Electron Microscopy
Department of Biology
Nassau Community College
Garden City, NY 11530
Voice Mail: (516) 572-7829
Email: {becks-at-sunynassau.edu}
URL: {http://www.sunynassau.edu/webpages/biology/becks.htm}




From daemon Sun Apr 16 16:44:45 2000



From: Steve Beck :      becks-at-sunynassau.edu
Date: Sun, 16 Apr 2000 12:50:22 -0400
Subject: Summer 2000 - TEM Course Announcement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


SUMMER I 2000 COURSE ANNOUNCEMENT - Transmission Electron Microscopy
(BIO. 221-Section B)

NASSAU COMMUNITY COLLEGE, Garden City, Long Island, New York

A five week, Summer Session I 2000 semester, course in Biological
Transmission Electron Microscopy is being offered by the Biology Department
of Nassau Community College. This is a 4 credit course offered four days
per week (Monday through Thursday) between the hours of 8:00 am and NOON.
Classes will begin on May 22 and end on June 22, 2000.

This is a "hands-on" course emphasizing biological specimen preparation,
ultra-thin sectioning involving block trimming, glass knifemaking and
operation of the ultramicrotomes (Sorvall MT-2B and LKB Ultrotome III),
thick and ultra-thin section mounting and contrast staining (UA and Pb
citrate), grid support films (formvar, carbon), student operation of the
TEM (Hitachi HS-8, Philips EM 300) and production of electron micrographs
through the process of black & white photography, and electron micrograph
analysis. Students will work on a chosen sample(s) with the goal of
producing a portfolio of high quality TEM photomicrographs of that
sample(s).

The course is widely transferrable and the cost per credit is reasonable at
$86 per credit (for Nassau County residents or New York State residents
with a certificate of residency).

More information about the Bio-Imaging Center at NCC, course descriptions
and syllabi, and the beginnings of a student gallery of EM
photomicrographs is available at our web site. The URL is
{http://www.sunynassau.edu/webpages/biology/becks.htm} .

Interested individuals should register as soon as possible since the course
is limited to a total enrollment of ten (10) students.

If you have further questions, you should e-mail me directly at the address
below.

For information about mail or telephone registration (Dial-a-Course) point
your browser to http://www.sunynassau.edu/courses/Sum00/index.html.

Stephen J. Beck
Associate Professor
Bio-Imaging Center/Electron Microscopy
Department of Biology
Nassau Community College
Garden City, NY 11530
Voice Mail: (516) 572-7829
Email: {becks-at-sunynassau.edu}
URL: {http://www.sunynassau.edu/webpages/biology/becks.htm}




From daemon Sun Apr 16 18:54:34 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Sun, 16 Apr 2000 16:41:21 -0700
Subject: Olympus BX DIC and phase LM systems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have two new condition Olympus LM systems for sale.

Transmitted DIC:

BX60 stand
trinoc head
2ea 10X eyepieces
6-place DIC nosepiece
DIC slider
DIC analyzer
Universal condenser
Rt stage
DIC inserts for 20, 40, 100X
UPlan FL 4X, 10X, 20X, 40X, 100X objectives
100 Watt lamphouse

Like new condition throughout.


Transmitted phase contrast:

BX50 stand
trinoc head
2ea 10X eyepieces
6-place nosepiece
Phase condenser
Phase inserts
Rt stage
UPlan FL Phase 4X, 20X, 40X, 60X, 100X, Plan 10X objectives
100 Watt lamphouse

Like new condition throughout.

Please contact me if you are interested in either of
these system. Telecon is 916.791.8191

I also have two Olympus PM10AD computer control photo
systems which include the control unit, shutter body,
focusing telescope and connecting cable. PE eyepieces
are also available.

tnx,
gary g.



From daemon Sun Apr 16 22:44:01 2000



From: arthurmott-at-netzero.net ()
Date: Sun, 16 Apr 2000 22:36:27 -0500
Subject: Bausch & Lomb Question:

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Can anyone answer this?

Nestor

--------------------------------------------------------------------------

Email: arthurmott-at-netzero.net
Name: Arthur Mott

Question: I have a Bausch & Lomb 0.7X-3X which I wish to convert to
gemological use(viewing gem stones). What type of lighting systems should
I use , or where can I gather additional information.

Arthur

---------------------------------------------------------------------------




From daemon Mon Apr 17 08:06:01 2000



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Mon, 17 Apr 2000 16:49:00 GMT+1200
Subject: Off and On

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi, Lou

}
}
} I agree with Bill Tivol. It is less stressfull on electrical circuits and
} contacts to leave the power on. To do so prolongs the life of the part, as
} well as the reliability of voltage flow through an instrument. A good
} example is XRD and XRF tubes. Rule #1 I learned as a grad student was to
} always leave the power on, keep a current flowing through the tubes. Why?
}
} A power current "burns in" at a single point on the tube. If the power is
} shut off, the burn in point can drift. Drift actually weakens the tube,
} which is the opposite of what you would expect. The drift will also cause
} current fluctuations, creating another source of error reducing the
} precision and accuracy of the measurements.
}
}

This is a pretty interesting way of looking at things, but it seems
like an argument for leaving things eg X-Ray tubes running at their
working conditions (kV and mA) fulltime.
Can you expand on this "single point"?
Is it some physical point on, for example, a filament, or is it kind
of metaphorical?
I can't quite understand the mechanism of the phenomenon you are
describing.
I've always just thought that the reason for leaving X-Ray tubes on,
but at minimum power, was to avoid the current inrush through a
cold (and therefore low-resistance) filament, plus the thermal
stresses in repeatedly warming up and cooling down of the tube and
all its glass-to-metal seals..

cheers

rtch

Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand



From daemon Mon Apr 17 08:06:02 2000



From: COURYHOUSE-at-aol.com
Date: Mon, 17 Apr 2000 02:02:06 EDT
Subject: Re: Bausch & Lomb Question:

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


hi there Arthur,
it is a dark field method of illumination and gemological assn. of America
sells a base that the b and l pod will fit. there are also some third parties
that do this base also but any of them can be pricey.
check out gia on the net. also try search under key gemscope
thanks Ed Sharpe archivist for SMECC



From daemon Mon Apr 17 08:06:07 2000



From: Anaspec :      anaspec-at-icon.co.za
Date: Mon, 17 Apr 2000 12:47:23 +0200
Subject: Petroleum Ether/spirit as a degreaser.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all

Whilst working in Australia we found a few labs using something called
Shellite as a de-greaser to clean microscope parts. This works very well and
seems to be very available. we then looked for the same product here in SA.
We then found that this is a trade name only in Australia and is actually
petroleum ether or spirit.
We called the local Shell distributor to ask what the difference was between
spirit and ether. Well, they were not sure on that one. Then we were told
you must specify the temperature range of the petroleum Ether. 40 - 60, 60 -
80 or 80 - 100 deg Celsius. Again no explanation as to what the difference
is an why.
Can any one give us some more info on Petroleum ether / spirit and the
advantages disadvantages on the different temperatures.


Thanks

Luc Harmsen
Anaspec, South Africa
Technical support on microscopy.
Tel + 27 (0) 11 476 3455
Fax + 27 (0) 11 476 7290
anaspec-at-icon.co.za
www.anaspec.co.za

Remember, ICEM 15 will be in
2002, Durban, South Africa.
www.icem15.com



From daemon Mon Apr 17 08:06:10 2000



From: Lou Solebello :      microls1297-at-mindspring.com
Date: Monday, April 17, 2000 4:56 AM
Subject: Off and On

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


It is a real physical phenomonom. I dont think I understand it correctly
myself, except if I think about like a spark plug in a car. You are right
about the vacuum, but take a look at some of your old tubes if you have any.
You should be able to see a gray smoky discoloration of the glass tubing at
some area on the tube, usually on one side near the center. That is the burn
in point.

That also brings up a curiosity question to me....Does it happen to the
newer cermaic tubes? I dont know, and will have to ask. The newer ceramic
tubes are supposed to have a longer life and reliability compared to the
glass ones.
-----Original Message-----
} From: Ritchie Sims {r.sims-at-auckland.ac.nz}
To: Lou Solebello {microls1297-at-mindspring.com} ;
Microscopy-at-sparc5.microscopy.com {Microscopy-at-sparc5.microscopy.com}


Hi, Lou

}
}
} I agree with Bill Tivol. It is less stressfull on electrical circuits and
} contacts to leave the power on. To do so prolongs the life of the part, as
} well as the reliability of voltage flow through an instrument. A good
} example is XRD and XRF tubes. Rule #1 I learned as a grad student was to
} always leave the power on, keep a current flowing through the tubes. Why?
}
} A power current "burns in" at a single point on the tube. If the power is
} shut off, the burn in point can drift. Drift actually weakens the tube,
} which is the opposite of what you would expect. The drift will also cause
} current fluctuations, creating another source of error reducing the
} precision and accuracy of the measurements.
}
}

This is a pretty interesting way of looking at things, but it seems
like an argument for leaving things eg X-Ray tubes running at their
working conditions (kV and mA) fulltime.
Can you expand on this "single point"?
Is it some physical point on, for example, a filament, or is it kind
of metaphorical?
I can't quite understand the mechanism of the phenomenon you are
describing.
I've always just thought that the reason for leaving X-Ray tubes on,
but at minimum power, was to avoid the current inrush through a
cold (and therefore low-resistance) filament, plus the thermal
stresses in repeatedly warming up and cooling down of the tube and
all its glass-to-metal seals..

cheers

rtch

Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand




From daemon Mon Apr 17 08:06:10 2000



From: Dr Eric Lachowski :      che136-at-abdn.ac.uk
Date: Mon, 17 Apr 2000 14:24:50 +0100 (BST)
Subject: petroleum ether

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The difference between photobleaching and quenching is quite fundamental.

Basically when a molecule is photobleached it is converted to another
molecule that is not fluorescent -i.e. a reaction takes place in which the
fluorescing species is converted to another species.

Quenching on the other hand results from the excited fluorescent molecules
having a non radiative route to release the energy it would typically
release as fluorescence. The state of the molecule after the quenching
however is the same as if it had fluoresced.
----------------------------------------------------------------------------
---------------------------
Dr. Giles Sanders
Zeneca / SmithKline Beecham Centre for Analytical Sciences
Chemistry Department
Imperial College of Science, Technology and Medicine
London
SW7 2AY

(44) - 0171-594-5749



Never express yourself more clearly than you think.
-- Niels Bohr (1885-1962) Danish physicist

----------------------------------------------------------------------------
------------------------
----- Original Message -----
} From: {"catchanangel-at-aol.com"-at-sparc5.microscopy.com}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: 15 April 2000 03:10


Luc,
The terms "petroleum spirit" and "petroleum ether" are
interchangeable. You may also encounter "light petroleum"
and "pet ether". The numbers correspond to the boiling
ranges in degrees celsius - they are just differnt
fractional distillates. The 40-60 fraction is
roughly hexane ( plus small amounts of larger and smaller
hydrocarbon molecules). The higher boiling fraction will
not evaporate as cleanly as the lighter ones, but all are
good solvents for grease and oil. They are, of course,
highly flammable.

Regards,
Eric

----------------------
Dr Eric Lachowski
Department of Chemistry
University of Aberdeen
Meston Walk
Old Aberdeen AB24 3UE
Scotland
+44 (0)1224 272934 fax 272921
e.lachowski-at-abdn.ac.uk





From daemon Mon Apr 17 11:36:06 2000



From: Philip Oshel :      peoshel-at-facstaff.wisc.edu
Date: Mon, 17 Apr 2000 08:35:11 -0500
Subject: Re: Bausch & Lomb Question:

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Check the web site for the Gemmological Institute of America (GIA). I
forget the exact URL, it's at home (www.gia.com? .org?). They sell a
stereoscope designed for exactly this purpose, and looking at it will
give you a good idea of what you need.

Briefly, it uses separate transmitted and reflected light sources.
The transmitted light I believe is an "ordinary" tungsten microscope
bulb, and the reflected source is a closely mounted fluorescent
source. The stones are held by a "third-hand" type of gripper so that
it can be examined from all angles. This is important not just in
studying inclusions, but because the optical characteristics
(including color) of some gems change with the crystal axis (the
Usambara effect, if I've spelled that right). Different types of
light are also needed, as color-change gems show different colors
depending on the incident light. For best effect, I would also had an
optical fiber source with dual goosenecks (not a ring-light, except
in addition), and a *good* mirror to shine sunlight on the specimen.
This is for judging stone color as well as color change.

The GIA site is a good source of information, but I'd check the Nat.
Mus. Natural Hist./Smithsonian, and the Mus. Nat. Hist. in London
also, to start.

Phil

} Can anyone answer this?
}
} Nestor
}
} --------------------------------------------------------------------------
}
} Email: arthurmott-at-netzero.net
} Name: Arthur Mott
}
} Question: I have a Bausch & Lomb 0.7X-3X which I wish to convert to
} gemological use(viewing gem stones). What type of lighting systems should
} I use , or where can I gather additional information.
}
} Arthur
}
} ---------------------------------------------------------------------------

--
}}}}}}}}}}}}}}}}}{{{{{{{{{{{{{{{{{
Philip Oshel
Supervisor, AMFSC and BBPIC
Dept. of Animal Health and Biomedical Sciences
University of Wisconsin
1656 Linden Drive
Madison, WI 53706-1581
voice: (608) 263-4162
fax: (608) 262-7420 (dept. fax)



From daemon Mon Apr 17 11:36:06 2000



From: Philip Oshel :      peoshel-at-facstaff.wisc.edu
Date: Mon, 17 Apr 2000 08:37:53 -0500
Subject: Re: Bausch & Lomb Question:

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Naturally I forgot the darkfield. (#*&$(&# Probably because it's important.

Phil

} hi there Arthur,
} it is a dark field method of illumination and gemological assn. of America
} sells a base that the b and l pod will fit. there are also some third parties
} that do this base also but any of them can be pricey.
} check out gia on the net. also try search under key gemscope
} thanks Ed Sharpe archivist for SMECC



From daemon Mon Apr 17 11:36:15 2000



From: Dr. Hodi Klara :      hodi-at-pharma.szote.u-szeged.hu
Date: Mon, 17 Apr 2000 17:49:00 MET
Subject: e-mail

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Dear colleagues,

some days I have any e-mail massage, perhaps my address was delated.
Write, please, my address again.

Best regards,

Dr. Klara Pintye-Hodi



From daemon Mon Apr 17 11:36:16 2000



From: Caroline Schooley :      schooley-at-mcn.org
Date: Mon, 17 Apr 2000 08:43:49 -0700
Subject: Re: Bausch & Lomb Question:

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

What do you mean by "convert"? Are you referring to a "dissecting" scope?
If so, you multiply the number that you've given (presumabvly the power of
the objective lens) times the power of the eyepiece, which is often 10x.
That would mean that you may have 30x available, and that definitely
requires good illumination. My son-in-law, who is a professional diamond
cutter, really likes the flexibility and brightness of fiber optic
illuminators - but they're expensive. The Edmund Optical catalog is a good
place to begin. If you want specific gemological advice, contact the
Gemological Institute of America (GIA).

Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/PCI/pci.html




From daemon Mon Apr 17 18:35:32 2000



From: Michael Bode :      mb-at-Soft-Imaging.com
Date: Mon, 17 Apr 2000 10:33:29 -0600
Subject: EM - Magnetic field strength in SEMs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Chris,

I don't think, there is an easy answer to your question. It depends on
the microscope type (immersion lens or not), the lens configuration and
design, and last but not least on the working distance, aside from the
lens current, which depends on the acceleration voltage.

I have a few pointers for you, though. I remember faintly a book by
Glaser, but that may be in German (anybody with a good reference for
this book?).

Another book is: Ludwig Reimer (Scanning Electron Microscopy : Physics
of Image Formation and Microanalysis (2nd Ed)(Springer Series in Optical
Sciences, Vol 45) )

Here is a URL for the book at Amazon.com:
http://www.amazon.com/exec/obidos/ASIN/3540639764/qid=955982926/sr=1-17/
102-8036949-3232817

This book is a bit "theoretical", but has information about electron
optics in the first two chapters.

You may want to search the net for "electron optics".

Hope this helps.

Michael

(disclaimer: I have no interest in Amazon.com. Check out other
bookstores for prices)

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Chris Walker [mailto:chris.walker-at-physics.org]
Sent: Friday, April 14, 2000 8:38 AM
To: 'Microscopy-at-MSA.Microscopy.Com'


Can anyone tell me the typical magnetic field strength at the sample in
an
SEM and (if possible) how fast the field drops off with distance from
the
axis?. Any references would be gratefully received.

Regards
Chris Walker



From daemon Mon Apr 17 18:35:39 2000



From: COURYHOUSE-at-aol.com
Date: Mon, 17 Apr 2000 16:02:29 EDT
Subject: Re: Bausch & Lomb Question:

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The pod, i.e., the top part that is the business end just comes out of the
stand of a b&l zoom and drops in the gia base...

Of course for adventure one can build the illuminating base necessary by
hand if so inclined.

Ed Sharpe

{ { Subj: Re: Bausch & Lomb Question:
Date: 4/17/00 11:41:32 AM US Mountain Standard Time
From: schooley-at-mcn.org (Caroline Schooley)
To: arthurmott-at-netzero.net
CC: Microscopy-at-sparc5.microscopy.com

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America
To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
-----------------------------------------------------------------------.


} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------------------
}
} Email: arthurmott-at-netzero.net
} Name: Arthur Mott
}
} Question: I have a Bausch & Lomb 0.7X-3X which I wish to convert to
} gemological use(viewing gem stones). What type of lighting systems should
} I use , or where can I gather additional information.
}
} Arthur -

What do you mean by "convert"? Are you referring to a "dissecting" scope?
If so, you multiply the number that you've given (presumabvly the power of
the objective lens) times the power of the eyepiece, which is often 10x.
That would mean that you may have 30x available, and that definitely
requires good illumination. My son-in-law, who is a professional diamond
cutter, really likes the flexibility and brightness of fiber optic
illuminators - but they're expensive. The Edmund Optical catalog is a good
place to begin. If you want specific gemological advice, contact the
Gemological Institute of America (GIA).

Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microsc } }



From daemon Mon Apr 17 18:35:44 2000



From: Douglas Keene :      DRK-at-shcc.org
Date: Mon, 17 Apr 2000 14:37:48 -0700 (Pacific Daylight Time)
Subject: ISI (Topcon) Service contract

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I am in need of a service contract for an ISI DS-130 SEM.
Is there anyone who is particularly happy with a contract
on their ISI (Topcon) instrument?
----------------------
Douglas R. Keene
Associate Investigator
Shriners Hospital Research Facilities
3101 S.W. Sam Jackson Park Road
Portland, Oregon 97201
phone: 503-221-3434
FAX: 503-412-6894 (9-5 PST)
e-mail: DRK-at-shcc.org




From daemon Mon Apr 17 18:35:47 2000



From: Walck, Scott D. :      walck-at-ppg.com
Date: Mon, 17 Apr 2000 18:25:16 -0400
Subject: RE: EELS: need spectrum for comparing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Try the following EELS database site. The have a number of O containing
materials there with all of the acquisition parameters well documented.
http://www.cemes.fr/eelsdb/

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)



} -----Original Message-----
} From: Jo Verbeeck [mailto:joverbee-at-ruca.ua.ac.be]
} Sent: Tuesday, April 11, 2000 10:09 AM
} To: Microscopy listserver message adress
} Subject: EELS: need spectrum for comparing
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.} html
}
}
} --------------------------------------------------------------
} ---------.
}
}
} Hello,
}
} I`m looking for an EELS spectrum containing Oxygen K-edge and some
} other edge like Mn or Ti L-edge. Together with low loss
} spectrum and data
} about the angles.
} The spectra need to have a power of 2 (1024 for inst.) bins
} and low loss
} and high loss should have same size.
} I want to use this for comparison, i`m trying to do some
} quantitative EELS
} work but so far with limited success.
} I want to find out wether my program or my data is wrong;)
}
} Thanks,
}
} Jo
}
} *************************************************************
} * Jo Verbeeck *
} * University of Antwerp *
} * Dept. EMAT (Electron Microscopy for Materials Research) *
} * e-mail: joverbee-at-ruca.ua.ac.be *
} * tel: +32(0)3 218 02 49 *
} * fax: +32(0)3 218 02 57 *
} *************************************************************
}
}



From daemon Mon Apr 17 18:35:48 2000



From: Kate Luby-Phelps :      lubyphel-at-SWVX12.SWMED.EDU
Date: Mon, 17 Apr 2000 15:37:28 -0500
Subject: Digital Imaging Add-on for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have a JEOL 840A scanning scope with an Oxford Link X-ray analysis
system. We would like to be able to get digital images out of it, for as
few $$ as possible. I would be interested in comments on the following
questions:

1) passive vs. active acquisition (my impression is that the resolution
is better with active, but how much?)

2) relative merits of different commercially available systems (dPict,
4PI, GW Electronics, etc).

3) Are NIH Image and Photoshop sufficient for analysis and processing of
images?

4) relative merits of various X-ray analysis software (such as FLAME,
etc).

Thanks very much.

Kate L-P
--
Kate Luby-Phelps
Molecular & Cellular Imaging Facility
UT Southwestern Medical Center
5323 Harry Hines Blvd.
Dallas, TX 75390-9039
e-mail: lubyphel-at-utsw.swmed.edu
Telephone: (214) 648-2190
Fax: (214) 648-6408 or -8885




From daemon Mon Apr 17 19:55:43 2000



From: jeffc07-at-hotmail.com ()
Date: Mon, 17 Apr 2000 19:39:53 -0500
Subject: Ask-A-Microscopist LM of E. coli and P. mirabilis

Contents Retrieved from Microscopy Listserver Archives
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Email: jeffc07-at-hotmail.com
Name: Jeff Courter
School: central michigan universty

Question: my problem is this: I have E. coli and P. mirabilis in 1% low
temperature gelling agarose in spurr's. is there any way to visualize the
specimen to trim it on a microtome other than taking sections individually
and staining them until i find the agar and bacteria.

---------------------------------------------------------------------------




From daemon Mon Apr 17 19:55:43 2000



From: jim :      jim-at-proscitech.com.au
Date: Tue, 18 Apr 2000 10:50:28 +1000
Subject: RE: Petroleum Ether/spirit as a degreaser.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I understand that Shellite is a Shell Petroleum Co trade name, the generic name
in Australia is white gas and this product is very similar to petrol (car fuel)
- except it does not have the certain additives. ( I ran in desperation a
Landcruiser for 50km on that stuff some years ago) It works well enough as a
general solvent for a first degreasing and cleaning of EM parts, especially
pumps. Its quiet cheap to purchase.
Petroleum Ether much more volatile and a more powerful solvents. (more
explosive too)
They may be the same chemically, except that white gas would have a
considerably higher boiling point than Pet. Ether.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Monday, April 17, 2000 8:47 PM, Anaspec [SMTP:anaspec-at-icon.co.za] wrote:
}
}
}
} Hi all
}
} Whilst working in Australia we found a few labs using something called
} Shellite as a de-greaser to clean microscope parts. This works very well and
} seems to be very available. we then looked for the same product here in SA.
} We then found that this is a trade name only in Australia and is actually
} petroleum ether or spirit.
} We called the local Shell distributor to ask what the difference was between
} spirit and ether. Well, they were not sure on that one. Then we were told
} you must specify the temperature range of the petroleum Ether. 40 - 60, 60 -
} 80 or 80 - 100 deg Celsius. Again no explanation as to what the difference
} is an why.
} Can any one give us some more info on Petroleum ether / spirit and the
} advantages disadvantages on the different temperatures.
}
}
} Thanks
}
} Luc Harmsen
} Anaspec, South Africa
} Technical support on microscopy.
} Tel + 27 (0) 11 476 3455
} Fax + 27 (0) 11 476 7290
} anaspec-at-icon.co.za
} www.anaspec.co.za
}
} Remember, ICEM 15 will be in
} 2002, Durban, South Africa.
} www.icem15.com



From daemon Tue Apr 18 07:14:41 2000



From: donald j marshall :      dmrelion-at-world.std.com
Date: Mon, 17 Apr 2000 22:28:53 -0400 (EDT)
Subject: subscribe

Contents Retrieved from Microscopy Listserver Archives
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subscribe



Donald J. Marshall
Relion Industries
P.O. Box 12
Bedford, MA 01730
Ph: 781-275-4695
FAX: 781-271-0252
email dmrelion-at-world.std.com

Cathodoluminescence, mass spectroscopy, electron beam technology


"A weed is a flower out of place."

(Please note: Do not send email with attachments to this address. Instead, send it to donbarlen-at-aol.com. Thank you.)



From daemon Tue Apr 18 07:14:45 2000



From: Jill Verlander :      verlaj-at-medicine.ufl.edu
Date: Tue, 18 Apr 2000 09:04:52 -0500
Subject: service on ISI-DS130

Contents Retrieved from Microscopy Listserver Archives
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Kate,
I saw your message on the Listserver. Here is my opinion on Digital
Imaging.

Passive vs. Active - Passive resolution is limited to the maximum
resolution the SEM can produce. If your scan generator has a maximum
resolution of 2000 lines for the photo scan, then your passive resolution
will also be 2000 lines. Passive just simply reads what's there already,
including any character, text and markers. What you see on the Viewing CRT
is "what you get" on the Passive System computer display. One neat thing
that a Passive System can do is grab an image in split screen mode, one side
a SE or BSE Image and the other half an x-ray dot map. An Active System, on
the other hand, takes direct control of the scan coils(replacing the scan
gen. in the sem), and digitizes the resulting video. Systems such as
DIGISEM, can achieve images of 4K x 4K. I believe the 4Pi system goes even
higher. I'm not sure about the rest. Active systems are typically suited
for fast, high quality digital images, that are then viewed and modified
with a program such as PhotoShop. Then, finally you have the standard TV
Rate Frame Grabber. This only works in "TV" mode, and your resolution is
typically limited to the resolution of the SEM TV scan rate(512 x 512?).
Slow scan, either Active or Passive is the way to go. Do it yourself Active
and Passive systems start out around $8,000.00, and of course, go up from
there.

NIH IMAGE was originally written as a MAC application. However, it has
been ported to the PC platform. In the process, the program has lost some
of its functionality. PhotoShop, for both the PC and MAC is a superior
program overall. There are some "shareware" programs out there worth
looking at, such as Paint Shop Pro. It's cheap(less than $100) and can do
just about anything PhotoShop can do(my opinion). Also, be aware that many
imaging systems include image analysis function. This really drives the
price up. If all you are interested in doing is simply grabbing and saving
an image, I would stay away from the "high end" systems.

The 4Pi imaging system is available both in a MAC and PC version. I
think everybody else is PC based.

EDS - What a can of worms. It seems everybody(including myself), offers
a "PC based EDS upgrade". I have personally used the PGT Avalon(formerly
American Nuclear Systems) and 4Pi Flame. The PGT Avalon eXcalibur software
in its latest release is full 32 bit and can run on the Win95/98 and NT
Workstation 4.0. PGT corrected many software problems after purchasing ANS.
Very nice package. The Flame software uses "fuzzy logic" in its approach
to quantitative analysis. The latest rendition of the Flame software seems
pretty good, however, the MAC version is somewhat more stable. Also, an
excellent product, along with 5***** customer support. Keep an eye out on
4Pi though, something new and exciting is in the works. Both are great
systems, inexpensive and easy to operate. Again, the 4Pi system is
available on both the PC and MAC platform. You will find prices on systems
such as these are very close in price to each other. Prices for these
"upgrades" typically start out around $15,000.00.
Good luck in your quest for the right system.

Gary M. Easton
Scanners Corporation
www.scannerscorp.com


Note: I am not employed by any of the companies listed above(except for
Scanners Corporation). However, I do have a commercial interest in these
products, because I sell them and this is how I make a living(along with
servicing SEM's). If I have slighted anybody, or any company, I apologize
in advance. The opinions and facts I have stated here are a result of my
22+ years experience in the scanning electron microscopy field


----- Original Message -----
} From: Kate Luby-Phelps {lubyphel-at-SWVX12.SWMED.EDU}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Monday, April 17, 2000 4:37 PM


Douglas - We have Image Control, Inc., based in Orlando, FL, service our
ISI-DS130C. We don't have a contract, just "as needed" service. I have
been very happy with service and pricing. They do offer service
contracts as well. I don't know what their geographical range is.
Phone number is (407) 234-0676; fax (407) 292-7802.

Jill
Jill Verlander Reed, D.V.M.
Associate Scientist
Director, College of Medicine Electron Microscopy Core Facility
University of Florida
P.O. Box 100215
Gainesville, FL 32610
verlaj-at-medicine.ufl.edu
Phone: (352) 846-0820
Fax: (352) 846-3299



From daemon Tue Apr 18 17:43:33 2000



From: Michael Bode :      mb-at-Soft-Imaging.com
Date: Tue, 18 Apr 2000 07:46:58 -0600
Subject: Digital Imaging Add-on for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Kate,

let me try to answer some of your questions. I am sending this cc to the
listserver as some of your questions come up on a regular basis.

1) passive vs. active:
Both types of acquisition will give you probably good images. The
difference is, that in a passive system, the computer (or digitizer)
simply digitizes the signals from the microscope. That means, that you
are limited to whatever the microscope can supply in terms of
resolution, dwell time, aspect ratio, etc. Most SEMs have a 1000 line or
2000 line option for taking images. That's what you get. A 1000x1200
image (for 1000 lines), or a 2000x2400 image (for 2000 lines). Plus of
course the other modes of the microscope (slow scan, fast scan, etc). An
active system actually takes control of the scanning coils, usually by
replacing the scan generator during PC controlled acquisition. Thus the
PC can select the resolution, dwell time, aspect ratio, etc. This gives
you much more freedom in selecting an image format, plus it is much
easier to acquire X-ray dot maps with an existing X-ray system. Typical
max. resolutions are 4000x4000. These big images take time to acquire,
though (seconds). Installation of a passive system may be easier in some
circumstances, but on your 840 the installation of an active system is
"plug and play".

2) different commercial systems.
As we sell our own system (ADDA II), and since this goes to the
listserver, I don't want to comment on this question. Call me if you
need information about our system.

3) Software:
Of the two programs you mention, I would prefer NIH. Photoshop is mainly
for making photos look nicer and may not have all the tools you require
(unless you buy other add-ons). Buy the software, however, with an eye
on expansion and support. Our experience with other users is, that once
they have added a digital acquisition system, they quickly find other
things they can do with the system and sometimes need more software or
hardware. For example, they realize that the old light microscope used
for specimen preparation can be digitized too and be used much better
than before. That, for example, would require a camera. So, if that is
the case, you want to look for a system that also supports cameras.
Another issue is the distribution of images. Many of our customers have
predefined "reports" for their customers. In that case you want to look
for software that supports the creation of custom templates, not just
printing images. An embedded, network capable database with search
capabilities is a big plus if you have several users.

4) X-ray software:
This is outside my area of expertise, so I will refrain from making any
comments.

I hope, this helps you decide about your SEM. If you have more detailed
questions, please send me an email or call me.

Michael

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================

*******************************************************
Disclaimer:
Soft Imaging System produces and sells image acquisition
and processing systems. We therefore have a vested
interest in some of the items mentioned above.
*******************************************************


-----Original Message-----
} From: Kate Luby-Phelps [mailto:lubyphel-at-SWVX12.SWMED.EDU]
Sent: Monday, April 17, 2000 2:37 PM
To: Microscopy-at-sparc5.microscopy.com


We have a JEOL 840A scanning scope with an Oxford Link X-ray analysis
system. We would like to be able to get digital images out of it, for as
few $$ as possible. I would be interested in comments on the following
questions:

1) passive vs. active acquisition (my impression is that the resolution
is better with active, but how much?)

2) relative merits of different commercially available systems (dPict,
4PI, GW Electronics, etc).

3) Are NIH Image and Photoshop sufficient for analysis and processing of
images?

4) relative merits of various X-ray analysis software (such as FLAME,
etc).

Thanks very much.

Kate L-P
--
Kate Luby-Phelps
Molecular & Cellular Imaging Facility
UT Southwestern Medical Center
5323 Harry Hines Blvd.
Dallas, TX 75390-9039
e-mail: lubyphel-at-utsw.swmed.edu
Telephone: (214) 648-2190
Fax: (214) 648-6408 or -8885




From daemon Tue Apr 18 17:43:34 2000



From: William Tivol :      tivol-at-wadsworth.org
Date: Tue, 18 Apr 2000 10:30:09 -0400
Subject: Re: Petroleum Ether/spirit as a degreaser.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


jim wrote:

Dear Jim,

} the generic name
} in Australia is white gas and this product is very similar to petrol (car fuel)
} - except it does not have the certain additives.

White gas is the petroleum distillate fraction used to make petrol.


} They may be the same chemically, except that white gas would have a
} considerably higher boiling point than Pet. Ether.

White gas contains hydrocarbons that have a boiling point roughly
the same as that of octane. There are some chemical differences, since the
higher-boiling-point fractions will contain more complex mixtures than
the lower-boiling-point fractions. In particular, the lowest such fraction,
which contains mostly pentanes, has no aromatics.
Yours,
Bill Tivol



From daemon Tue Apr 18 17:43:35 2000



From: donald j marshall :      dmrelion-at-world.std.com
Date: Tue, 18 Apr 2000 10:44:33 -0400 (EDT)
Subject: subscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


subscribe Microscopy dmrelion-at-world.std.com




Donald J. Marshall
Relion Industries
P.O. Box 12
Bedford, MA 01730
Ph: 781-275-4695
FAX: 781-271-0252
email dmrelion-at-world.std.com

Cathodoluminescence, mass spectroscopy, electron beam technology


"A weed is a flower out of place."

(Please note: Do not send email with attachments to this address. Instead, send it to donbarlen-at-aol.com. Thank you.)



From daemon Tue Apr 18 17:43:36 2000



From: Jeremy Sanderson :      jb_sanderson-at-yahoo.com
Date: Tue, 18 Apr 2000 07:53:22 -0700 (PDT)
Subject: For C Singla

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Following the thread on processing tissue-culture
cells for TEM, would C Singla reply back to me if they
would like a protocol for embedding cell monolayers
for TEM in the petri-dish.

Jeremy Sanderson

jb_sanderson-at-yahoo.com

__________________________________________________
Do You Yahoo!?
Send online invitations with Yahoo! Invites.
http://invites.yahoo.com



From daemon Tue Apr 18 17:43:38 2000



From: Phillip Rutledge :      prutledge-at-ars.usda.gov
Date: Tue, 18 Apr 2000 09:28:54 -0600
Subject: LM - tissue processors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I'm currently in the process of setting up a Histology/Microbiology/EM facility. Being out of Histology for almost 30 years (been doing EM) I'm not sure of the best type of tissue processor to buy. I won't be doing that many specimens, so I'm leaning towards a rotary tissue processor. I have info on Shandon and Tissue-Tek. Are there others out there? Anyone having opinions on the 2 mentioned, I would appreciate any comments. I am looking for ease of use (changing solutions, fume safeguards), reliability on service and cost effectiveness. Thanks.

Peace be with you,
Phil Rutledge (410)778-4136, 2120
prutledge-at-ars.usda.gov







From daemon Tue Apr 18 17:43:39 2000



From: donald j marshall :      dmrelion-at-world.std.com
Date: Tue, 18 Apr 2000 11:43:28 -0400 (EDT)
Subject: subscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


subscribe Microscopy dmrelion-at-world.std.com



From daemon Tue Apr 18 17:43:48 2000



From: Marti, Jordi :      jordi.marti-at-honeywell.com
Date: Tue, 18 Apr 2000 11:44:30 -0700
Subject: Folding Grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have been using folding grids for my sections for a long time (200 mesh,
Cu). Lately however I have found it very difficult to fold the grids so
that the two sides overlap properly and only a few of the holes are in good
registry, the rest are partially blocked by the overlap. I am wondering if
this is a manufacturing problem ( i.e. a bad batch ?) . Has anyone else
experienced this problem with a batch of grids ? Suggestions are welcome.

Thanks,

Jordi Marti



From daemon Tue Apr 18 17:43:49 2000



From: Jeff Doran :      jeffd-at-di.com
Date: Tue, 18 Apr 2000 13:36:39 -0700
Subject: AFM seminar/workshop at Princeton University

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


There will be an electrical characterization by AFM seminar/workshop sponsored
by Digital Instruments and Princeton University on April 25, 2000. For
additional information and registration, please visit the DI web site at
www.di.com and click on "workshops and seminars".

Regards,
Jeff Doran
Digital Instruments
Veeco Metrology Group





From daemon Tue Apr 18 17:43:50 2000



From: Jeff Doran :      jeffd-at-di.com
Date: Tue, 18 Apr 2000 13:35:08 -0700
Subject: AFM Seminar/Workshop at Princeton University

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


There will be an electrical characterization by AFM seminar/workshop sponsored
by Digital Instruments and Princeton University on April 25, 2000. For
additional information and registration, please visit the DI web site at
www.di.com and click on "workshops and seminars".

Regards,
Jeff Doran
Digital Instruments
Veeco Metrology Group





From daemon Tue Apr 18 17:43:50 2000



From: Scott D. Davilla :      davilla-at-4pi.com
Date: Tue, 18 Apr 2000 16:44:35 -0500
Subject: RE: Digital Imaging Add-on for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} though (seconds). Installation of a passive system may be easier in some
} circumstances, but on your 840 the installation of an active system is
} "plug and play".

Actually, about 90% of the 840s have the internal scan relays
soldered in place on the scan gen board. The other 10% will need the relays
added. JEOL service is best for this as they have the relays in stock. To
check, one must physically examine the scan gen board.

Scott
-----------------------------------------------------------------------
Scott D. Davilla Phone: 919 489-1757 (tel)
4pi Analysis, Inc. Fax: 919 489-1487 (fax)
3500 Westgate Drive, Suite 403 email: davilla-at-4pi.com
Durham, North Carolina 27707-2534 web: http://www.4pi.com



From daemon Tue Apr 18 17:43:51 2000



From: Philip Oshel :      peoshel-at-facstaff.wisc.edu
Date: Tue, 18 Apr 2000 16:06:51 -0500
Subject: Value of a used Hitachi S-570

Contents Retrieved from Microscopy Listserver Archives
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Anyone have an idea what the sale value might be for a Hitachi S-570
with a LaB6 gun & solid-state backscatter detector? No x-ray. Comes
with a Gatan digital imaging system. Sorry, but I don't know the
model of this system, but it uses a PowerMac with a NuBus card, so
it's a bit hoary.

In good condition, recently PMed to give "to spec." performance in
the upper stage at 200,000X.

Thanks.

Phil
--
}}}}}}}}}}}}}}}}}{{{{{{{{{{{{{{{{{
Philip Oshel
Supervisor, AMFSC and BBPIC
Dept. of Animal Health and Biomedical Sciences
University of Wisconsin
1656 Linden Drive
Madison, WI 53706-1581
voice: (608) 263-4162
fax: (608) 262-7420 (dept. fax)



From daemon Tue Apr 18 17:43:52 2000



From: Lewis McCrigler :      lmm7001-at-Humboldt.edu
Date: Tue, 18 Apr 2000 14:17:00 -0700
Subject: Help on an Orthomat E Photo System

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,
I'm looking for technical assistance or a service manual for a Leitz
Orthomat E (7916) Photo System.The problem we are having is the unit will
not allow a picture to be taken unless there is very little light. Once the
system thinks it is in range, there is not enough light present to get the
photograph. An adjustment of the light detector? Any assistance is
appreciated.
Lewis McCrigler
Humboldt State University



From daemon Wed Apr 19 08:05:27 2000



From: John Nailon :      mmjnailo-at-dingo.cc.uq.edu.au
Date: Mon, 17 Apr 2000 09:21:05 +1000
Subject: Re: Petroleum Ether/spirit as a degreaser.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


G'day Luc,
Shellite is a solvent manufactured by Shell, its used as stove fuel,
lighter fuel, drycleaning solvent and as a rubber/adhesive solvent
(also, as you have found, a great EM parts cleaner). Another name you
may find Shellite listed under is Shell X55 solvent.
Shellite is basically a highly volitile, low octane, unleaded petrol.
According to Shell Australia it is not the same as white spirit, this is
less volitile than shellite, therefore harder to evaporate from the
metal surface.
Below is part of the safety data sheet for Shellite. If anyone requires
the complete 10 page safty data sheet please email me.
Luc, see you next time you are in OZ.
Regards
JVN
SHELL SHELlLITE

STATEMENT OF HAZARDOUS NATURE

HAZARDOUS ACCORDING TO WORKSAFE AUSTRALIA CRITERIA

SUPPLIER

Company: The Shell Company of Australia Limited
Address: Shell House, 1 Spring Street (PO Box 872K) PO Box 2091
Melbourne Wellington
VIC 3001 New Zealand
Australia
Telephone: (03)9666 5444
Telephone: 64 4 4720080
Fax: (03)96665008/64 44980100


HAZARD RATINGS

Flammability: 4

Toxicity: 2

Body Contact: 2

Reactivity: 0

Chronic effect: 2

Scale: Min / Nil = 0, Low = 1, Moderate = 2, High = 3 and Extreme = 4.

PERSONAL PROTECTIVE EQUIPEMENT FOR
INDUSTRIAL/COMMERCIAL ENVIRONMENTS

Product Name:
Shell Shellite
Other Names:
Product Code 00720


CAS RN No(s):
None
U.N. Number:
1268
Packaging Group:
II
Dangerous Goods Class:
3.1
Subsidiary Risk:
None
Hazchem Code:
3[Y]E
Poisons Schedule Number:
S5


USE

Used as rubber solvent, cleaning solvent, lighter fluid and as fast
evaporating, highly volatile solvent in
enamels, adhesives and lacquers. The use of a quantity of material in an
unventilated or confined
space may result in increased exposure and an irritating atmosphere
developing Before starting
consider control of exposure by mechanical ventilation.

PHYSICAL DESCRIPTION/PROPERTIES

APPEARANCE

Clear highly flammable liquid with a typical hydrocarbon liquid odour;
floats on water. Classed as an
aliphatic solvent; i.e has low aromatic content.

Molecular Weight:
Not applicable.
Boiling Point (deg C):
47-128
Melting Point (deg C):
Not available.
Vapour Pressure (kPa):
34.5 -at- 15 deg C
Specific Gravity:
0.71 -at- 15 deg C
Flash Point (deg C):
{-30
Lower Explosive Limit (%):
1.0
Upper Explosive Limit (%):
7.5
Solubility in Water (g/L):
Immiscible


INGREDIENTS

NAME
CAS RN
%
paraffins, as
liquid hydrocarbons
Various
} 60
naphthenes
Notspec
n-hexane
110-54-3
13
aromatic hydrocarbons total, including
{5.0
toluene
108-88-3
3.5app
ethylbenzene
100-41-4
benzene
71-43-2
{0.5
C8 and higher aromatics
approx1

Anaspec wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hi all
}
} Whilst working in Australia we found a few labs using something called
} Shellite as a de-greaser to clean microscope parts. This works very well and
} seems to be very available. we then looked for the same product here in SA.
} We then found that this is a trade name only in Australia and is actually
} petroleum ether or spirit.
} We called the local Shell distributor to ask what the difference was between
} spirit and ether. Well, they were not sure on that one. Then we were told
} you must specify the temperature range of the petroleum Ether. 40 - 60, 60 -
} 80 or 80 - 100 deg Celsius. Again no explanation as to what the difference
} is an why.
} Can any one give us some more info on Petroleum ether / spirit and the
} advantages disadvantages on the different temperatures.
}
} Thanks
}
} Luc Harmsen
} Anaspec, South Africa
} Technical support on microscopy.
} Tel + 27 (0) 11 476 3455
} Fax + 27 (0) 11 476 7290
} anaspec-at-icon.co.za
} www.anaspec.co.za
}
} Remember, ICEM 15 will be in
} 2002, Durban, South Africa.
} www.icem15.com

--
****************************************************
John V Nailon
Operations Manager
Centre for Microscopy and Microanalysis
The University of Queensland
St. Lucia Queensland 4072
Phone: +61-7-3365-4214
Fax: +61-7-3365-4422
WWW: http://www.uq.edu.au/nanoworld/allstaff.html#Nailon
****************************************************



From daemon Wed Apr 19 08:05:36 2000



From: Ford Royer :      froyer-at-bitstream.net
Date: Tue, 18 Apr 2000 21:13:05 -0500
Subject: Re: LM - tissue processors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I carry a full line of refurbished histology equipment. Please contact me for price quotes and recommendations.

Ford Royer
Analytical Instruments
9921 13th Ave. N.
Minneapolis, MN 55441
(800) 565-1895, extension 17
fax: (612) 929-1895
email: froyer-at-bitstream.net

Phillip Rutledge wrote:

} ------------------------------------------------------------------------
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} -----------------------------------------------------------------------.
}
} I'm currently in the process of setting up a Histology/Microbiology/EM facility. Being out of Histology for almost 30 years (been doing EM) I'm not sure of the best type of tissue processor to buy. I won't be doing that many specimens, so I'm leaning towards a rotary tissue processor. I have info on Shandon and Tissue-Tek. Are there others out there? Anyone having opinions on the 2 mentioned, I would appreciate any comments. I am looking for ease of use (changing solutions, fume safeguards), reliability on service and cost effectiveness. Thanks.
}
} Peace be with you,
} Phil Rutledge (410)778-4136, 2120
} prutledge-at-ars.usda.gov



From daemon Wed Apr 19 08:05:38 2000



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Wed, 19 Apr 2000 16:10:51 GMT+1200
Subject: White Spirit

Contents Retrieved from Microscopy Listserver Archives
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Just to throw in a further little complication of nomenclature:

In New Zealand, (and maybe in Australia, too), the general name we
use for stuff like Shellite, is "white spirit".

In the US it's often called "unleaded gas(oline)", or "white
gas(oline)", I think.

It's the stuff that Coleman camping stoves run on.

However, in the UK, "white spirits" is what I would call "mineral
turpentine" ie the comparitively non-volatile solvent often used for
thinning oil-based paints.

As I found a few years ago, shortly after my arrival in the UK to
continue the camping holiday that I'd started in the US, it doesn't
work in Coleman stoves.

rtch

Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand



From daemon Wed Apr 19 08:06:08 2000



From: Massimo Catalano :      massimo.catalano-at-ime.le.cnr.it
Date: Wed, 19 Apr 2000 09:16:35 +0200
Subject: TEM of clusters and image analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listservers,

we have recently started working on Transmission Electron Microscopy of
several kinds of clusters in various martices.
We frequently face the problem of analyzing particle size distribution and
other features of the images.

We use several software programs for our particle analysis, but we always
conclude that measuring the particle size by eye is the most reliable way
to get accurate data.

We have both Digital Micrograph software and other programs (Scion image
etc...), but we have never used them for this kind of application.

As we look both at bulk materials, and at thin films or powders, frequently
our images show an uneven contrast, and defining a threshold is difficult.

Can anyone suggest (and eventually share) a script, a program or a set of
user functions that allows to study particle size distributions?

Thank very much in advance for your help in this matter.

Max

Dr. Massimo Catalano
CNR-IME
Campus Universitario
Via Arnesano
73100 Lecce - ITALY
tel: + 39 0832 322362
fax: + 39 0832 325299
email: massimo.catalano-at-ime.le.cnr.it
http://www.ime.le.cnr.it
http://www.ime.le.cnr.it/sime/sime.htm






From daemon Wed Apr 19 08:06:11 2000



From: Sidorov, Max :      msv-at-nl.feico.com
Date: Wed, 19 Apr 2000 13:10:05 +0200
Subject: RE: TEM of clusters and image analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Massimo:
Try to Fourier-filter (high-pass) the images before doing the particle
analysis. In this way you might be able remove the influence of the uneven
background. Sometimes this works nicely. You can easily do it in Digital
Micrograph.
Hope this helps,

Max Sidorov
----------------------------
Dr. Maxim V. Sidorov
TEM Applications Specialist
Philips Electron Optics, Applications Laboratory
Building AAE, Achtseweg Noord 5
5600 MD Eindhoven, the Netherlands

e-mail: msv-at-nl.feico.com
Phone: +31-40/2766101
Fax: +31-40/2766102



} -----Original Message-----
} From: Massimo Catalano [SMTP:massimo.catalano-at-ime.le.cnr.it]
} Sent: Wednesday, April 19, 2000 09:17
} To: Microscopy-at-sparc5.microscopy.com
} Subject: TEM of clusters and image analysis
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear Listservers,
}
} we have recently started working on Transmission Electron Microscopy of
} several kinds of clusters in various martices.
} We frequently face the problem of analyzing particle size distribution and
}
} other features of the images.
}
} We use several software programs for our particle analysis, but we always
} conclude that measuring the particle size by eye is the most reliable way
} to get accurate data.
}
} We have both Digital Micrograph software and other programs (Scion image
} etc...), but we have never used them for this kind of application.
}
} As we look both at bulk materials, and at thin films or powders,
} frequently
} our images show an uneven contrast, and defining a threshold is difficult.
}
} Can anyone suggest (and eventually share) a script, a program or a set of
}
} user functions that allows to study particle size distributions?
}
} Thank very much in advance for your help in this matter.
}
} Max
}
} Dr. Massimo Catalano
} CNR-IME
} Campus Universitario
} Via Arnesano
} 73100 Lecce - ITALY
} tel: + 39 0832 322362
} fax: + 39 0832 325299
} email: massimo.catalano-at-ime.le.cnr.it
} http://www.ime.le.cnr.it
} http://www.ime.le.cnr.it/sime/sime.htm
}
}
}
}



From daemon Wed Apr 19 08:44:13 2000



From: donald j marshall :      dmrelion-at-world.std.com
Date: Wed, 19 Apr 2000 08:28:05 -0500
Subject: LEO 440i SEM and cathodoluminescence (CL)

Contents Retrieved from Microscopy Listserver Archives
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To SEM users and vendors

On a recent business trip outside the US, I met some people who are using a
LEO 440i SEM. They wanted to do cathodoluminescence (CL) with it and were
not having too much success so I volunteered to post this message to the
list to seek advice. The PMT is mounted in a housing/port that is located
at
about 2 O'clock (if you consider the door to be at 6 o'clock). The mounting
of the PMT is horizontal and it looks like the distance to the PMT input is
about 20 cm from the center of the chamber. There is a glass lens on a
slider arrangement in front of the PMT input but they were unclear as to
how
to adjust this.

They do not know the type of PMT that was supplied and haven't opened up
the system to determine this. It looks like it must be an end-on type.

One of their questions has to do with the adjustment, if any, for the PMT
voltage and how they can do this and how they can monitor the value of the
PMT voltage.

They believe that the PMT should have been mounted at an angle to better
see
the sample but the only other port where this could be done is not usable
for the PMT when the EDS detector is in place.

The other work that they are doing with the instrument is completely
satisfactory, so far as I could tell, and I suspect that someone on the
list
can provide the answers to these questions and help to get them in business
doing monochromatic CL as well.

All suggestions from ohter users of this type of instrument, obviously,
will
be much appreciated.

Any vendors who provide CL accessories for this instrument should contact
me
directly.

Thank you.

Donald J. Marshall

Donald J. Marshall
Relion Industries
P.O. Box 12
Bedford, MA 01730
Ph: 781-275-4695
FAX: 781-271-0252
email dmrelion-at-world.std.com

Cathodoluminescence, mass spectroscopy, electron beam technology


"A weed is a flower out of place."

(Please note: Do not send email with attachments to this address. Instead,
send it to donbarlen-at-aol.com. Thank you.)




From daemon Wed Apr 19 18:24:03 2000



From: Jeff Doran :      jeffd-at-di.com
Date: Wed, 19 Apr 2000 07:23:57 -0700
Subject: AFM Seminar/Workshop at PMI

Contents Retrieved from Microscopy Listserver Archives
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Please note that the Seminar/Workshop at Princeton University on April 25, 2000
will be at PMI. For additional information and registration please see the DI
web site at www.di.com and click on "Workshops and Seminars".

Regards,
Jeff Doran
Digital Instruments
Veeco Metrology Group



From daemon Wed Apr 19 18:24:04 2000



From: Bruce Brinson :      brinson-at-cnst.rice.edu
Date: Wed, 19 Apr 2000 09:15:13 -0500
Subject: Re: White Spirit

Contents Retrieved from Microscopy Listserver Archives
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Hi Richie & readers
As I recall from the oil field days of the 80s, the term "white
gasoline" referred to a condensate from natural gas production. People were
known to collect (swipe) this free fuel at the well site & use it in their
autos. I do not know what it's composition is but this was not really
considered a good substitute for gasoline. Something about destroyed pistons
& the such.

Bruce Brinson
Rice U.

Ritchie Sims wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Just to throw in a further little complication of nomenclature:
}
} In New Zealand, (and maybe in Australia, too), the general name we
} use for stuff like Shellite, is "white spirit".
}
} In the US it's often called "unleaded gas(oline)", or "white
} gas(oline)", I think.
}
} It's the stuff that Coleman camping stoves run on.
}
} However, in the UK, "white spirits" is what I would call "mineral
} turpentine" ie the comparitively non-volatile solvent often used for
} thinning oil-based paints.
}
} As I found a few years ago, shortly after my arrival in the UK to
} continue the camping holiday that I'd started in the US, it doesn't
} work in Coleman stoves.
}
} rtch
}
} Ritchie Sims Phone : 64 9 3737599 ext 7713
} Department of Geology Fax : 64 9 3737435
} The University of Auckland email : r.sims-at-auckland.ac.nz
} Private Bag 92019
} Auckland
} New Zealand



From daemon Wed Apr 19 18:24:05 2000



From: Michael Bode :      mb-at-Soft-Imaging.com
Date: Wed, 19 Apr 2000 08:39:20 -0600
Subject: RE: Digital Imaging Add-on for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Yes, but Kate said, that they already have an EDX system attached.
Normally these systems need access to the scan coils as well, that's why
I mentioned "plug and play". I bet, that the EDX system is connected to
connector JA2 on the back of the microscope, which is where we would
also connect the digital acquisition system (of course with an
additional connector for the existing X-ray system).

Michael

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Scott D. Davilla [mailto:davilla-at-4pi.com]
Sent: Tuesday, April 18, 2000 3:45 PM
To: Microscopy-at-sparc5.microscopy.com


} though (seconds). Installation of a passive system may be easier in
some
} circumstances, but on your 840 the installation of an active system is
} "plug and play".

Actually, about 90% of the 840s have the internal scan relays
soldered in place on the scan gen board. The other 10% will need the
relays
added. JEOL service is best for this as they have the relays in stock.
To
check, one must physically examine the scan gen board.

Scott
-----------------------------------------------------------------------
Scott D. Davilla Phone: 919 489-1757 (tel)
4pi Analysis, Inc. Fax: 919 489-1487 (fax)
3500 Westgate Drive, Suite 403 email: davilla-at-4pi.com
Durham, North Carolina 27707-2534 web: http://www.4pi.com



From daemon Wed Apr 19 18:24:07 2000



From: Chris Edwards :      fishon-at-umich.edu
Date: Wed, 19 Apr 2000 11:42:18 -0400
Subject: Zeiss Axiocam

Contents Retrieved from Microscopy Listserver Archives
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Has anyone had a demo for the new Zeiss CCD known as the "Axiocam"? Or
better, has anyone ordered it and had it installed in their lab. I'd like
to know the usual things regarding performance and overall satisfaction.
Thanks.




From daemon Wed Apr 19 18:24:08 2000



From: Chris Jeffree :      cjeffree-at-srv0.bio.ed.ac.uk
Date: Wed, 19 Apr 2000 17:06:23 +0000
Subject: Cause of turbo pump failure

Contents Retrieved from Microscopy Listserver Archives
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Some of you asked what caused the turbo pump failure I related
last week. I frankly don't know. There is some scope in the S250
for objects (samples, for example) to fall into the pump. Cambridge
fitted a mesh screen to trap these, but it is possible that some
small hard fragment got between the fan and stator blades
somehow. In early Cambridge 250s there was no rough pumping
sequence - the baffle valve was simply opened, dumping the
specimen chamber air at 1 bar into the turbo pump while it was
running at its top speed. The dramatic shreik of protest that
results is a great party trick when demonstrating the machine to
visitors, but slows the pump dramatically and undoubtedly causes
great mechanical stress on the turbines and stator blades.
Strangely, we never suffered pump failures during this stressful
event, but only when the pumps were in apparently smooth running
at top speed. I think we are now on about our fourth pump in
twenty years, and this last one has survived unscathed for about 10
years with nothing more than an occasional oil-change, during
which time it has mostly been run continuously, surviving endless
air-dump cycles. Now that I have told you that I can probably
expect our next failure almost immediately! Perhaps I'll order the
skip now, just in case.
=====================================================================
DR CHRIS JEFFREE
BIOSEM - BIOLOGICAL SCIENCES EM FACILITY
UNIVERSITY OF EDINBURGH
Daniel Rutherford Building
King's Buildings, Mayfield Road
EDINBURGH, EH9 3JH, Scotland, UK
Tel. #44 131 650 5345
FAX. #44 131 650 6563
Mobile 0410 585 401
email c.jeffree-at-ed.ac.uk
SEM / TEM bookings sem-at-ed.ac.uk
=====================================================================



From daemon Wed Apr 19 18:24:09 2000



From: tom moninger :      moninger-at-emiris.iaf.uiowa.edu
Date: Wed, 19 Apr 2000 11:22:23 -0500 (CDT)
Subject: Re: Zeiss Axiocam

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Chris,

I saw the scope several weeks back but never saw through it, Zeiss could
not get it to work. I know one lab on campus has one and they say it
images beautifully, when it works...... they have been using our
Optronics lately.

Thomas Moninger moninger-at-emiris.iaf.uiowa.edu
University of Iowa Central Microscopy Research Facility
http://www.uiowa.edu/~cemrf
Views expressed are mine.
On Wed, 19 Apr 2000, Chris Edwards wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Has anyone had a demo for the new Zeiss CCD known as the "Axiocam"? Or
} better, has anyone ordered it and had it installed in their lab. I'd like
} to know the usual things regarding performance and overall satisfaction.
} Thanks.
}
}
}



From daemon Wed Apr 19 18:24:09 2000



From: Don Grimes :      microtoday-at-mindspring.com
Date: Wed, 19 Apr 2000 12:21:46 -0500
Subject: Just For Fun Image Contest

Contents Retrieved from Microscopy Listserver Archives
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Readers,
At the upcoming Microscopy & Microanalysis Conference (13/17 August in
Philadelphia) we will again hold a Just For Fun Image Contest. Concept is an
image composed of one or more other images, one of which must be
microscopical in nature.
Prizes will be $300, $200 and $100 for first, second and third prizes
respectively.
One does not have to be present to win.
Should you might be interested, kindly contact me direct and I will forward
detail.
Last year we had over 30 entries.
Regards,
Don Grimes, Microscopy Today



From daemon Wed Apr 19 18:24:13 2000



From: Scott D. Davilla :      davilla-at-4pi.com
Date: Wed, 19 Apr 2000 13:30:55 -0500
Subject: RE: Digital Imaging Add-on for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} Yes, but Kate said, that they already have an EDX system attached.
} Normally these systems need access to the scan coils as well, that's why
} I mentioned "plug and play". I bet, that the EDX system is connected to
} connector JA2 on the back of the microscope, which is where we would
} also connect the digital acquisition system (of course with an
} additional connector for the existing X-ray system).

Maybe you misunderstand my intent. All I'm saying is don't assume a
JEOL 840 has everything required for active scan control. Some don't, but
it's not a show stopper provided the information is known and planned for
in advance.
Just because there is an EDX system attached does not mean that it
is attached to the microscope beam control. There are more EDX systems sold
without X-ray mapping than sold with X-ray mapping. In the same note, just
because the JEOL 840 has a "plug and play" interface does not mean that the
external scan relays are present. Both issues raise flags here and can mean
the difference in a painless or painfull installation if the details are
not addressed.

Scott
-----------------------------------------------------------------------
Scott D. Davilla Phone: 919 489-1757 (tel)
4pi Analysis, Inc. Fax: 919 489-1487 (fax)
3500 Westgate Drive, Suite 403 email: davilla-at-4pi.com
Durham, North Carolina 27707-2534 web: http://www.4pi.com



From daemon Wed Apr 19 18:24:17 2000



From: Philip Oshel :      peoshel-at-facstaff.wisc.edu
Date: Wed, 19 Apr 2000 15:28:58 -0500
Subject: Value of the S-570

Contents Retrieved from Microscopy Listserver Archives
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Many thanks for the various valuations sent me. We have enough now
for our needs.
For those interested, the valuations ranged from 15k$ to 45k$, with
the average about 25 - 30 k$.

Phil
--
}}}}}}}}}}}}}}}}}{{{{{{{{{{{{{{{{{
Philip Oshel
Supervisor, AMFSC and BBPIC
Dept. of Animal Health and Biomedical Sciences
University of Wisconsin
1656 Linden Drive
Madison, WI 53706-1581
voice: (608) 263-4162
fax: (608) 262-7420 (dept. fax)



From daemon Wed Apr 19 18:24:17 2000



From: John Chandler :      chandler-at-lamar.ColoState.EDU
Date: Wed, 19 Apr 2000 14:57:38 -0700
Subject: Digital printer needs repair (Kodak XLS8300)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have a Kodak XLS8300 dye sublimation printer that needs repair. Kodak
no longer supports this instrument, has no replacement parts we need and
doesn't know where we can get service.

When we shipped it for repair, we got a report that said it needed a hard
drive and possibly a saturn board (motherboard, I presume) and that they
could not provide service.

I would appreciate getting suggestions of where to get this printer serviced.

Please reply directly to me, and not to the listserv.

Thanks,

John
chandler-at-colostate.edu




From daemon Wed Apr 19 18:24:18 2000



From: Steven Ridge :      s.ridge-at-fryerco.com
Date: Wed, 19 Apr 2000 16:10:41 -0500
Subject: Re.LM-Quartz coverslips

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Can someone help me with a US source to purchase, low fluorescence
quartz coverlips for conventional microscope slides. Please respond
directly at the following email address.

Steven Ridge
Fryer Company Inc.
847-669-2000 Phone
s.ridge-at-fryerco.com

--
BMv



From daemon Wed Apr 19 18:55:07 2000



From: Joe :      jmetzger-at-suscom.net
Date: Wed, 19 Apr 2000 19:51:19 -0400
Subject: Xray

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I recently completed a research paper to complete my EE
Degree which included a section on vacuum principles. I
incorporated part of the in formation I got from an article
by XEI scientific on contamination control. It spoke of oil
deposits on cool windows or a loss of low energy xray. I
thought I also read (on the web site) of how x-rays will
turn ceramics yellow. I can not find the article again.
Can you help me with this? What I am trying to decipher is,
will concentrated xray emission through a ceramic cause it
to yellow and could contamination deposits be the cause of
this? Would appreciate any answers or leads as to where I
might find the answer.

Sincerely
Joe Metzger




From daemon Wed Apr 19 19:04:30 2000



From: David Henriks :      Henriks-at-CompuServe.COM
Date: Wed, 19 Apr 2000 19:58:17 -0400
Subject: Extra rooms for MRS in San Francisco

Contents Retrieved from Microscopy Listserver Archives
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Once again there is a conference coming up and I have found myself with a
few extra rooms. If anyone is need of hotel rooms at the San Francisco
Marriott for the Materials Research Society meeting next week, please let
me know. If nobody needs them, I will be cancelling them tomorrow night.

I have rooms for arrival on Saturday April 22 and departure on Friday April
28. Of course the dates could be changed if needed. They are rooms with 2
double beds and the confrence rate is $138 I think.

Let me know ASAP.

Best regards-

David
Writing at 4:52:02 PM on 04/19/2000

***************************************************************************
************************

David Henriks
Vice President TEL: 800-728-2233
(toll free in the USA)
South Bay Technology, Inc. +1-949-492-2600
1120 Via Callejon FAX: +1-949-492-1499
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

***************************************************************************
************************

} } } } } Please visit us at http://www.southbaytech.com { { { { {

Manufacturers of precision sample preparation equipment and supplies for
metallography, crystallography and electron microscopy.



From daemon Thu Apr 20 07:37:23 2000



From: Ronald Vane :      RVaneXEI-at-concentric.net
Date: Wednesday, April 19, 2000 5:59 PM
Subject: Xray

Contents Retrieved from Microscopy Listserver Archives
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Dear Joe and list members:

I did not say anything about yellowing of ceramics at the XEI Scientific web
site. It is generally not a "contamination" problem. X-rays being an
ionizing radiation are able to break bond and modify the crystalline
structure of ceramics. When I did X-ray spectrometry I was very aware of the
ability of high intensity, high energy x-rays to cause radiation damage and
discoloration. However scanning electron microscope are generally operated
at low energies ( {30KeV) and low beam currents. Xray damage is not a problem
except with most sensitive materials.

Xray damage to oils could cause them to crosslink and yellow if oils are
deposited on the surface of ceramic but I have not seen any complaints about
this. Maybe a more specific description of your problem would help. Reply
off list.

Notice: XEI Scientific sells anti-contamination systems.

Ronald Vane
XEI Scientific

-----Original Message-----
} From: Joe {jmetzger-at-suscom.net}
To: Microscopy-at-sparc5.microscopy.com {Microscopy-at-sparc5.microscopy.com}



From daemon Thu Apr 20 07:37:24 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Wed, 19 Apr 2000 21:33:07 -0700
Subject: Re: Re.LM-Quartz coverslips

Contents Retrieved from Microscopy Listserver Archives
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At 02:10 PM 4/19/00 , you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Rreally....can anyone identify a source of high quality
#1 cover slips that are not made in China and don't have
micro fractures in them?

gg



From daemon Thu Apr 20 17:36:19 2000



From: jim :      jim-at-proscitech.com.au
Date: Thu, 20 Apr 2000 22:32:57 +1000
Subject: RE: Re.LM-Quartz coverslips

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Coverslip number 1 thickness is 0.13 to 0.17mm. Ours are 0.25 thick (and 25.4mm
dia)
I didn't know #1 quartz coverslips existed, made in China or outer space.
Perhaps GG should not blame the Chinese manufacturer for flaws in #1 slips;
maybe perfect #1 in quartz are impossible.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Thursday, April 20, 2000 2:33 PM, Dr. Gary Gaugler [SMTP:gary-at-gaugler.com]
wrote:
}
}
} At 02:10 PM 4/19/00 , you wrote:
}
} } Can someone help me with a US source to purchase, low fluorescence
} } quartz coverlips for conventional microscope slides. Please respond
} } directly at the following email address.
} }
} } Steven Ridge
} } Fryer Company Inc.
} } 847-669-2000 Phone
} } s.ridge-at-fryerco.com
} }
} } --
} } BMv
}
}
} Rreally....can anyone identify a source of high quality
} #1 cover slips that are not made in China and don't have
} micro fractures in them?
}
} gg
}



From daemon Thu Apr 20 17:36:19 2000



From: Purdy, Sam :      SPurdy-at-nationalsteel.com
Date: Thu, 20 Apr 2000 07:40:33 -0500
Subject: RE: White Spirit

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Friends:
I have always wondered about these solvents. One way to sort out
intercontinental differences is to ask - what do they smell like? Gasoline
has a distinctive odor, whether leaded or not. The Pet Ether I use in my lab
is nearly odorless. The label describes it as having a "boiling range of
37.7 to 55.8 C/ 1 drop to dryness." Turpentine, or more correctly, oil of
turpentine, has another distinctive odor. It is used to thin paints.
Petroleum paint thinner, a good solvent, is much like pet ether, nearly
odorless. All of the above are colorless, Kerosene, used in camp stoves and
cabin heaters, another petroleum derivative, is yellow and possesses its own
characteristic odor. All of these observations are from a US view point.
How do these other solvents smell?

Sam Purdy
National Steel Corp Tech Center
Trenton, MI, USA
spurdy-at-nationalsteel.com

} ----------
} From: Ritchie Sims
} Sent: 19, April 2000, 12:10 PM
} To: 'MSA listserver'
} Subject: White Spirit
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
}
} Just to throw in a further little complication of nomenclature:
}
} In New Zealand, (and maybe in Australia, too), the general name we
} use for stuff like Shellite, is "white spirit".
}
} In the US it's often called "unleaded gas(oline)", or "white
} gas(oline)", I think.
}
} It's the stuff that Coleman camping stoves run on.
}
} However, in the UK, "white spirits" is what I would call "mineral
} turpentine" ie the comparitively non-volatile solvent often used for
} thinning oil-based paints.
}
} As I found a few years ago, shortly after my arrival in the UK to
} continue the camping holiday that I'd started in the US, it doesn't
} work in Coleman stoves.
}
} rtch
}
} Ritchie Sims Phone : 64 9 3737599 ext 7713
} Department of Geology Fax : 64 9 3737435
} The University of Auckland email : r.sims-at-auckland.ac.nz
} Private Bag 92019
} Auckland
} New Zealand
}




From daemon Thu Apr 20 17:36:20 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Thu, 20 Apr 2000 06:17:41 -0700
Subject: RE: Re.LM-Quartz coverslips

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


OK, if not quartz, how about glass? The Chinese products
come pre-made with micro cracks. Erie used to make slips
but stopped. I can find round slips from Swiss glass but not
square ones for 1" x 3" slides.

I think that 4 attempts with Chinese covers which all have the
same problem is not a coincidence. These were bought from
Ward's Scientific. They exchanged them without any hassle.
The problem is that the slips are un useable. I'd be glad to
send a few new ones to you so you can see the cracks for
yourself.

gary g.


At 05:32 AM 4/20/00 , you wrote:
} Coverslip number 1 thickness is 0.13 to 0.17mm. Ours are 0.25 thick (and
} 25.4mm
} dia)
} I didn't know #1 quartz coverslips existed, made in China or outer space.
} Perhaps GG should not blame the Chinese manufacturer for flaws in #1 slips;
} maybe perfect #1 in quartz are impossible.
} Cheers
} Jim Darley
} ProSciTech Microscopy PLUS
} PO Box 111, Thuringowa QLD 4817 Australia
} Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
} Great microscopy catalogue, 500 Links, MSDS, User Notes
} www.proscitech.com
}
} On Thursday, April 20, 2000 2:33 PM, Dr. Gary Gaugler [SMTP:gary-at-gaugler.com]
} wrote:
} }
} }
} } At 02:10 PM 4/19/00 , you wrote:
} }
} } } Can someone help me with a US source to purchase, low fluorescence
} } } quartz coverlips for conventional microscope slides. Please respond
} } } directly at the following email address.
} } }
} } } Steven Ridge
} } } Fryer Company Inc.
} } } 847-669-2000 Phone
} } } s.ridge-at-fryerco.com
} } }
} } } --
} } } BMv
} }
} }
} } Rreally....can anyone identify a source of high quality
} } #1 cover slips that are not made in China and don't have
} } micro fractures in them?
} }
} } gg
} }



From daemon Thu Apr 20 17:36:21 2000



From: jim :      jim-at-proscitech.com.au
Date: Thu, 20 Apr 2000 23:50:47 +1000
Subject: RE: Re.LM-Quartz coverslips

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I cannot believe that only Chinese coverslips are sold in the USA. We carry a
comprehensive range of good German-made coverslips . Yes, and they don't have
common flaws.
If that sounds too much like an advertisement, I'll add that several suppliers
in Australia offer a similar range. Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Thursday, April 20, 2000 11:18 PM, Dr. Gary Gaugler [SMTP:gary-at-gaugler.com]
wrote:
} OK, if not quartz, how about glass? The Chinese products
} come pre-made with micro cracks. Erie used to make slips
} but stopped. I can find round slips from Swiss glass but not
} square ones for 1" x 3" slides.
}
} I think that 4 attempts with Chinese covers which all have the
} same problem is not a coincidence. These were bought from
} Ward's Scientific. They exchanged them without any hassle.
} The problem is that the slips are un useable. I'd be glad to
} send a few new ones to you so you can see the cracks for
} yourself.
}
} gary g.
}
}
} At 05:32 AM 4/20/00 , you wrote:
} } Coverslip number 1 thickness is 0.13 to 0.17mm. Ours are 0.25 thick (and
} } 25.4mm
} } dia)
} } I didn't know #1 quartz coverslips existed, made in China or outer space.
} } Perhaps GG should not blame the Chinese manufacturer for flaws in #1 slips;
} } maybe perfect #1 in quartz are impossible.
} } Cheers
} } Jim Darley
} } ProSciTech Microscopy PLUS
} } PO Box 111, Thuringowa QLD 4817 Australia
} } Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
} } Great microscopy catalogue, 500 Links, MSDS, User Notes
} } www.proscitech.com
} }
} } On Thursday, April 20, 2000 2:33 PM, Dr. Gary Gaugler
[SMTP:gary-at-gaugler.com]
} }
} } wrote:
} } }
} } }
} } } At 02:10 PM 4/19/00 , you wrote:
} } }
} } } } Can someone help me with a US source to purchase, low fluorescence
} } } } quartz coverlips for conventional microscope slides. Please respond
} } } } directly at the following email address.
} } } }
} } } } Steven Ridge
} } } } Fryer Company Inc.
} } } } 847-669-2000 Phone
} } } } s.ridge-at-fryerco.com
} } } }
} } } } --
} } } } BMv
} } }
} } }
} } } Rreally....can anyone identify a source of high quality
} } } #1 cover slips that are not made in China and don't have
} } } micro fractures in them?
} } }
} } } gg
} } }



From daemon Thu Apr 20 17:36:22 2000



From: ComCryLab1-at-aol.com
Date: Thu, 20 Apr 2000 10:18:03 EDT
Subject: Re: Re.LM-Quartz coverslips

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


}
}
} Can someone help me with a US source to purchase, low fluorescence
} quartz coverlips for conventional microscope slides. Please respond
} directly at the following email address.
}
} Steven Ridge
} Fryer Company Inc.
} 847-669-2000 Phone
} s.ridge-at-fryerco.com
}
Steven,
We can make anything you need. Email, fax or call with specifications.
Mike Urbanik
guru-at-crystalguru.com
www.crystalguru.com
Ph 941-645-5959
Fax 941-643-6058



From daemon Thu Apr 20 17:36:22 2000



From: Michael Bode :      mb-at-Soft-Imaging.com
Date: Thu, 20 Apr 2000 08:12:36 -0600
Subject: RE: Digital Imaging Add-on for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Scott,

I don't think we're in disagreement here. We've had our share of
surprises (SEMs with external connectors for beam control, then nothing
attached to the connectors, strange boards in the microscopes that
should not have been there, etc.) We have had very few problems with the
840 (and 820, 6300, 6400 for that matter). But of course there is always
a possibility that something is missing or not working, which must be
ascertained in each case individually. As you said, it is usually not a
show stopper, but requires some modifications of the microscope.

Michael

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Scott D. Davilla [mailto:davilla-at-4pi.com]
Sent: Wednesday, April 19, 2000 12:31 PM
To: Microscopy-at-sparc5.microscopy.com


} Yes, but Kate said, that they already have an EDX system attached.
} Normally these systems need access to the scan coils as well, that's
why
} I mentioned "plug and play". I bet, that the EDX system is connected to
} connector JA2 on the back of the microscope, which is where we would
} also connect the digital acquisition system (of course with an
} additional connector for the existing X-ray system).

Maybe you misunderstand my intent. All I'm saying is don't
assume a
JEOL 840 has everything required for active scan control. Some don't,
but
it's not a show stopper provided the information is known and planned
for
in advance.
Just because there is an EDX system attached does not mean that
it
is attached to the microscope beam control. There are more EDX systems
sold
without X-ray mapping than sold with X-ray mapping. In the same note,
just
because the JEOL 840 has a "plug and play" interface does not mean that
the
external scan relays are present. Both issues raise flags here and can
mean
the difference in a painless or painfull installation if the details are
not addressed.

Scott
-----------------------------------------------------------------------
Scott D. Davilla Phone: 919 489-1757 (tel)
4pi Analysis, Inc. Fax: 919 489-1487 (fax)
3500 Westgate Drive, Suite 403 email: davilla-at-4pi.com
Durham, North Carolina 27707-2534 web: http://www.4pi.com



From daemon Thu Apr 20 17:36:23 2000



From: Filion, Christian :      christian.filion-at-atlasstainless.com
Date: Thu, 20 Apr 2000 10:21:13 -0400
Subject: EDS spectra on PC

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Good morning,

I have both a Spectra file in Link format and txt format saved as a MSA
type file. Is there a software or an excel macro that could read these
spectra ?

Thank you

Christian Filion
Superviseur Laboratoires
Aciers Inoxydables Atlas
1640 Marie-Victorin
Tracy, QuŽbec
J3R 5R5
TelÊ: 450-746-5243
faxÊ: 450-746-5241



From daemon Thu Apr 20 17:36:23 2000



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Thu, 20 Apr 2000 10:51:25 -0500
Subject: Quartz coverslips availability

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Steven Ridge wrote:
==========================================================
Can someone help me with a US source to purchase, low fluorescence quartz
coverlips for conventional microscope slides. Please respond directly at
the following email address.
===========================================================
SPI Supplies has offered quartz coverslips 0.2 mm thick for some time and
the details can be found on URL
http://www.2spi.com/catalog/ltmic/quartz.html

The optical characteristics of (fused) quartz are very very sensitive to the
impurity levels present. Therefore it is important that one use coverslip
that are of reproducible quality and of known impurity levels (also linked
from above webpage). The particular quartz used for the manufacture of the
SPI quartz coverslips is the same "electronic grade" used in many
applications in the electronics industry. The coverslips and quartz are of
USA manufacture (just in case one might wonder about origin).

Disclaimer: SPI Supplies has supplied quartz coverslips to users in the
microscopy community for some number of years. Quartz coverslips are also
offered by several other of the leading suppliers of consumables to the
microscopy market.

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.spi.cc
########################
============================================




From daemon Thu Apr 20 17:36:24 2000



From: Valerie Leppert :      vjleppert-at-ucdavis.edu
Date: Thu, 20 Apr 2000 08:58:00 -0700 (PDT)
Subject: Unsubscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Please unsubscribe.



From daemon Thu Apr 20 17:36:26 2000



From: Anthony Garratt-Reed :      tonygr-at-mit.edu
Date: Thu, 20 Apr 2000 14:27:04 -0400
Subject: Re: EDS spectra on PC

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Christian and colleagues:

I take it you are referring to Link AN10000, QX2000 or eX/L spectra. There
are a number of ways to analyze these spectra post facto. All depend on
having them on a DOS disk, which presumably you do. If you have them on
LINK formatted floppys, then you can read them on a DOS machine with a
small program RDL2.EXE which I wrote a while ago, although that program
will not read subdirectories on LINK floppys. It copies the files
byte-by-byte to a DOS hard drive. Run the program to get three lines of
useage instructions.
Once they are in DOS, you can do a number of things:

1) Use my program LKSPCV.EXE which will convert them into a text file
formatted for direct input into Excel or another spreadsheet or graphing
program. LKSPCV knows about the differences between eX/L files and
AN10/QX2000 files, and I believe (though I've never tested) that it will
also handle the old LINK 860 files correctly, too. Usage: LKPSCV
infilename.sp outfilename.txt

2) Use the program DTSA (Desktop Spectrum Analyser) which used to be sold
by NIST. I know that the status of that program has changed, and I don't
know if it is still available or not. However, it runs on a Mac, and can
import both AN10/QX2000 and eX/L files, as well as MSA formatted files.

3) If you have access to a LINK ISIS analyser via a friend or colleague,
these systems can import and process eX/L or AN10/QX2000 spectrum files.

LKSPCV.EXE and RDL2.EXE are available by FTP from prism.mit.edu:2101. Ther
are a number of other files on that site, too, of which the useful ones are
LKCONV.COM which formats LINK disks on a PC (which must be running plain
DOS - not a DOS window) and LKCV3.EXE which extracts individual images and
maps from LINK eX/L or AN10/QX2000 studies.


At 10:21 AM 04/20/2000 -0400, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

** Anthony J. Garratt-Reed
** MIT Room # 13-1027
** 77 Massachusetts Avenue
** Cambridge, MA 02139-4307
** USA
**
** Phone: (+) 1-617-253-4622
** Fax: (+) 1-617-258-6479
**




From daemon Thu Apr 20 17:36:30 2000



From: Walck, Scott D. :      walck-at-ppg.com
Date: Thu, 20 Apr 2000 18:06:06 -0400
Subject: RE: EDS spectra on PC

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have a program that I wrote in Visual Basic 6 that will read the EMSA
format EDS and EELS data, plot them, color, them, overlay them, print them,
copies the graphical data to an RTF document, and can convert them to the
EMSA format with two column X,Y pairs. I have been toying with the idea of
selling the program through South Bay Technology inexpensively. I have no
idea what the market would be for something like this. I have given a few
copies of it out for evaluation and hoped to get feedback about what I
should add to it, but no one has gotten back to me. The program is
primarily geared for EELS spectra but works with EMIspec, Noran, and DTSA
EMSA formatted EDS spectra. If you agree to give me feedback on the program
(and a pitcher of beer at M&M 2000 if you go), I might be able to be
convinced to send you a copy. I would like to know if it works with some of
the other EDS systems.

The reason that I wrote it was that the Gatan EL/P program output the EMSA
format in y-only format with columns of five. Gatan isn't the only company
to do that, I believe that the Noran data comes out that way. That format
is difficult to parse properly in Excel so that you can graph it. What I
did was to open the ASCII data in a word processor, replace all the hard
returns with a comma, then replace all the commas with a hard return, and
then resave as an ASCII text file. Then you can open it in (or copy and
paste the data) in Excel. Then you move the column over once and create the
X values for plotting the data as X,Y pairs.

I originally wrote the program to just convert it to X,Y pairs so that I
could use Excel but then it was so easy to graph it in VB6, that I just got
carried away.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)


"The opinions expressed are those of Scott D. Walck and not of PPG
Industries, Inc. nor of any PPG-associated companies."
--


} -----Original Message-----
} From: Filion, Christian [mailto:christian.filion-at-atlasstainless.com]
} Sent: Thursday, April 20, 2000 10:21 AM
} To: microscopy-at-sparc5.microscopy.com
} Subject: EDS spectra on PC
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.} html
}
}
} --------------------------------------------------------------
} ---------.
}
}
} Good morning,
}
} I have both a Spectra file in Link format and txt format
} saved as a MSA
} type file. Is there a software or an excel macro that could read these
} spectra ?
}
} Thank you
}
} Christian Filion
} Superviseur Laboratoires
} Aciers Inoxydables Atlas
} 1640 Marie-Victorin
} Tracy, QuŽbec
} J3R 5R5
} TelÊ: 450-746-5243
} faxÊ: 450-746-5241
}



From daemon Thu Apr 20 21:43:49 2000



From: Robert.Carlton-at-aventis.com
Date: Thu, 20 Apr 2000 17:37:42 -0500
Subject: Duane Hunt Limit

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


To all,

I am trying to track down information on the short wavelength cut-off used
in EDS microanalysis to determine the true electron accelerating voltage. I
have a number of articles describing its determination, but little in the
way of historical information. It is also known as the 'Duane-Hunt' limit.
I cannot find any references to a Duane or a Hunt. Does anyone know the
originator of the concept and why it is known as this?


Thanks
Robert A. Carlton
Aventis Pharmaceuticals
Tel 610-454-3949
Robert.Carlton-at-aventis.com




From daemon Fri Apr 21 08:28:21 2000



From: Ben Craft :      bcraft-at-uci.edu
Date: Thu, 20 Apr 2000 20:51:30 -0700 (PDT)
Subject: x-ray microanalysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We are looking to upgrade our EDS system with a 4pi based system or Evax.
Does any one have any opinions about these systems or is there others you
could recomend?
The TEM is a CM20 with an EDAX detector.



#######
#####\_O -Ben Craft-
####/\/}
#### /"
### \





From daemon Thu Apr 20 17:36:25 2000



From: Nestor J. Zaluzec :      zaluzec-at-aaem.amc.anl.gov
Date: Thu, 20 Apr 2000 11:36:36 -0500
Subject: Re: EDS spectra on PC

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Christian

The MSA text format is written in such a way so that the data should be
directly importable into any standard spreadsheet program.

Just import it as ASCII text with a "comma" deliminator between columns.

I am presuming that you stored the data in 2 column (X,Y) format.
If you did otherwise you will need to do a small amount of cleanup
but it should not be difficult. The other obvious trick is to go
back to the EDS system and make sure you store the data in X,Y
single column format;

Nestor



} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


==================================================================
Nestor J. Zaluzec
Materials Science Division
Building 212
Argonne National Lab
9700 S. Cass Ave
Argonne, Illinois 60439 USA
Tel: 630-252-7901, Fax: 630-252-4798
Email: Zaluzec-at-aaem.amc.anl.gov
==================================================================
TPMLab: http://tpm.amc.anl.gov
MMSite: http://www.amc.anl.gov
==================================================================

The box said "This program requires Win 95/98/NT or better..." so I bought
a G3 Mac

==================================================================




From daemon Fri Apr 21 17:44:05 2000



From: Rwhentges-at-aol.com
Date: Fri, 21 Apr 2000 14:48:23 EDT
Subject: unsubscribe

Contents Retrieved from Microscopy Listserver Archives
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Please unsubscribe.



From daemon Fri Apr 21 17:44:06 2000



From: Ed Vicenzi :      vicenzi-at-volcano.si.edu
Date: Fri, 21 Apr 2000 16:22:12 -0400
Subject: Smithsonian SEM Lab Manager

Contents Retrieved from Microscopy Listserver Archives
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The SEM lab manager position at the Smithsonian Institution is now open.

The National Museum of Natural History in Washington, DC is seeking an
experienced electron microscopist to fill a vacancy for SEM laboratory
operation and management. The SEM facility is designed to serve both the
biological and geological research communities in the museum, and houses
two recent model SEMs and one state-of-the-art environmental microscope (to
be installed in mid-2000). The principal responsibilities include training
staff members and visiting scientists in proper use of equipment and theory
of electron generation and detection, maintenance and troubleshooting all
instrumentation (in conjunction with full service contracts), evaluation of
new developments in SEM technology, and supervision of a support staff
member. The successful applicant will also have the opportunity to gain
experience in Focused Ion Beam (FIB) Microscopy and high spatial resolution
secondary ion mass spectrometry HR SIMS.

This position will fill a federal government vacancy and is offered at the
GS11 ($42,724) or GS12 ($51,204) levels, and carries promotion potential to
grade GS 13. U.S. citizenship is required for this federal position. To
obtain information concerning this vacancy call our automated Jobline at
(202) 287-3102 (24 hours, 7 days a week), press 9 and request vacancy
announcement 00MQ-2069, then follow voice prompts to have information faxed
or mailed to you. The application deadline is May 16th, 2000. If
questions arise after receiving and reading through the vacancy
announcement please contact: Dr. Edward Vicenzi (Chair, Search Committee)
at vicenzi-at-volcano.si.edu. The Smithsonian Institution is an equal
opportunity employer.


NOTE: Completed Smithsonian applications must be received by May 16th, 2000.




~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Edward P. Vicenzi
Smithsonian Institution
Department of Mineral Sciences
Washington, DC 20560-0119

(202) 357-2594
(202) 357-2476 (fax)
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~



From daemon Sun Apr 23 09:07:39 2000



From: Wil Bigelow :      bigelow-at-engin.umich.edu
Date: Sat, 22 Apr 2000 13:31:10 -0400
Subject: RE: Short-Wavelength limit

Contents Retrieved from Microscopy Listserver Archives
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The short wavelength limit for x-ray spectra produced by excitation with an
electron beam is discussed and illustrated in the section on "The
Continuous Spectrum" (I believe Sect. 1.3) in Chapter 1 of The book
'Elements of X-ray Diffraction', by B. D. Cullity, Addison Wesley.

Basically, you are dealing here with the Bremsstrahlung radiation which is
generated by electrons in the incident electron beam which lose increments
of their energy as they interact with the specimen. Each incremental
energy loss gives rise to an x-ray photon having a photon energy equal to
the energy loss increment, i.e. Energy loss of a beam electron = Energy of
generated Bremsstrahlung photon. The maximum amount of energy a beam
electron electron can give up occurs when it is stopped in a single
collision, whereupon the energy loss equals the energy imparted to the
electron by the applied accelerating voltage, and is numerically the same
as the value of the accelerating voltage when expressed in keV. (i.e. an
accelerating voltage of 20 kV gives electrons a kinetic energy of 20 keV) A
maximum energy loss event of this kind produces a Bremsstrahlund photon
with the highest possible energy. Photon wavelengths decrease as photon
energies increase, and so this Bremsstrahlung photon of maximum energy also
is the photon with the shortest wavelength. Ergo, if you measure the
photon energy at the short wavelength limit, you know the value of the
accelerating voltage.

Wilbur C. Bigelow, Prof. Emeritus
Materials Sci. & Engr., University of Michigan
Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu;
Fx:734-763-4788; Ph:734-662-5237




From daemon Mon Apr 24 07:47:50 2000



From: jim :      jim-at-proscitech.com.au
Date: Mon, 24 Apr 2000 21:56:25 +1000
Subject: RE: Quartz coverslips purity

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Very few people use quartz coverslips, but they should not be afraid. I
understand that the only USA manufacturer is General Electric and almost
certainly all pure quartz products made in USA are made from quartz supplied by
GE. Our quartz slips and slides are made from GE quartz too.
I think that it is important for endusers to know when supplies are in fact
standard (however excellent) or "extra special".
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Friday, April 21, 2000 1:51 AM, Garber, Charles A. [SMTP:cgarber-at-2spi.com]
wrote:
}
} -- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --
}
} Steven Ridge wrote:
} ==========================================================
} Can someone help me with a US source to purchase, low fluorescence quartz
} coverlips for conventional microscope slides. Please respond directly at
} the following email address.
} ===========================================================
}
} . . . .The optical characteristics of (fused) quartz are very very sensitive
to the
} impurity levels present. Therefore it is important that one use coverslip
} that are of reproducible quality and of known impurity levels (also linked
} from above webpage). The particular quartz used for the manufacture of the
} SPI quartz coverslips is the same "electronic grade" used in many
} applications in the electronics industry. The coverslips and quartz are of
} USA manufacture (just in case one might wonder about origin).
}
} Disclaimer: SPI Supplies has supplied quartz coverslips to users in the
} microscopy community for some number of years. Quartz coverslips are also
} offered by several other of the leading suppliers of consumables to the
} microscopy market.
}
} Chuck



From daemon Mon Apr 24 18:23:28 2000



From: beth-at-dogwood.botany.uga.edu (Beth Richardson)
Date: Mon, 24 Apr 2000 11:01:14 -0500
Subject: digital cameras for copy stand work?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all,
This is off the microscopy subject...sorry.
Any recommendations on digital cameras for copy stand work and general
photography? Has anyone tried the Nikon Coolpix 990?
We're coming up on the end of the fiscal year and there might be funds
available for a digital camera so any advice is greatly appreciated.
thanks,
Beth Richardson

**************************************
Beth Richardson
EM Lab Coordinator
Botany Department
University of Georgia
Athens, GA 30602

Phone - (706) 542-1790
FAX - (706) 542-1805
Email - beth-at-dogwood.botany.uga.edu
**************************************




From daemon Mon Apr 24 18:23:28 2000



From: Scott Wight :      scott.wight-at-nist.gov
Date: Mon, 24 Apr 2000 11:01:21 -0400
Subject: Re: EDS spectra on PC

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all,
The status is now that DTSA is being given away free by NIST, go to
http://www.cstl.nist.gov/div837/837.02/dtsa.html
it is Macintosh only but imports many file types.
Scott

} Anthony Garratt-Reed wrote:
..snip...
} 2) Use the program DTSA (Desktop Spectrum Analyser) which used to be sold
} by NIST. I know that the status of that program has changed, and I don't
} know if it is still available or not. However, it runs on a Mac, and can
} import both AN10/QX2000 and eX/L files, as well as MSA formatted files.
..snip...


------------------- note: new mailing address ------------------------
Scott Wight fax: 301-417-1321
NIST 222/A113 W voice: 301-975-3949
100 Bureau Dr STOP 8371 | email: scott.wight-at-nist.gov
Gaithersburg, MD 20899-8371 \|/ disclaimer: Any opinion expressed
is my own and does not represent those of my employer.




From daemon Mon Apr 24 18:23:30 2000



From: sherwood-at-helix.mgh.harvard.edu (Peggy Sherwood)
Date: Mon, 24 Apr 2000 11:27:41 -0400
Subject: NESM Spring Symposium-Woods Hole

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


To all:

The 17th Annual New England Society for Microscopy (NESM) at Woods Hole, MA
will be held Friday,
May 12th and Saturday, May l3th.

Highlights of the meeting include: Invited Presentations in both the
Biological and Physical Sciences
arena, Commercial exhibits, Poster/Photomicrograph Award Contest, Banquet &
Cocktail Get-Together,
Discovery Cruise, Door Prizes, and much more!

For information re: registration, meeting agenda, poster/photomicrograph
submission forms, accomodations, and directions, please contact Peggy
Sherwood, Corresponding Secretary (NESM) at
MESnesm-at-aol.com. A newsletter will be mailed to you. Please respond
ASAP-registration deadline is
Monday, May 1, 2000!

Hope to see you at Woods Hole!

Peggy Sherwood
MESnesm-at-aol.com




From daemon Mon Apr 24 18:23:32 2000



From: RonMervis-at-aol.com
Date: Mon, 24 Apr 2000 13:11:07 EDT
Subject: kodak digital camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


hi all....
we are considering getting a kodak MDS 120 digital camera for photomicroscopy
(brightfield)...does anyone have any experience with this system...is it cost
effective....? user friendly?
Is there any other system that would be recommended?
thanks for any feedback
Ronald F. Mervis, Ph.D.
RonMervis-at-aol.com
~~~~~~~~~~~~~~~~
Neuro-Cognitive Research Laboratories
2109 West Fifth Ave
Columbus, OH 43212



From daemon Mon Apr 24 18:23:33 2000



From: Dusevich, Vladimir :      DusevichV-at-umkc.edu
Date: Mon, 24 Apr 2000 12:38:28 -0500
Subject: RE: Xray

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Long time ago I had worked in a laboratory which had
a linear electron accelerator. It was well known that
regular glasses (made from glass, not plastic) left
for a few days close to accelerator would turn in a
pretty good sunglasses. Effect would last for a few
month, and then glasses again would be clear. So,
every spring a few glasses were sitting close to accelerator.
Sure, energy was much higher than for SEM.

Vladimir Dusevich

} Dear Joe and list members:
}
} I did not say anything about yellowing of ceramics at the XEI
} Scientific web
} site. It is generally not a "contamination" problem. X-rays being an
} ionizing radiation are able to break bond and modify the crystalline
} structure of ceramics. When I did X-ray spectrometry I was
} very aware of the
} ability of high intensity, high energy x-rays to cause
} radiation damage and
} discoloration. However scanning electron microscope are
} generally operated
} at low energies ( {30KeV) and low beam currents. Xray damage
} is not a problem
} except with most sensitive materials.
}
} Xray damage to oils could cause them to crosslink and
} yellow if oils are
} deposited on the surface of ceramic but I have not seen any
} complaints about
} this. Maybe a more specific description of your problem would
} help. Reply
} off list.
}
} Notice: XEI Scientific sells anti-contamination systems.
}
} Ronald Vane
} XEI Scientific
}
} -----Original Message-----
} } From: Joe {jmetzger-at-suscom.net}
} To: Microscopy-at-sparc5.microscopy.com
} {Microscopy-at-sparc5.microscopy.com}
} Date: Wednesday, April 19, 2000 5:59 PM
} Subject: Xray
}
}
} } -------------------------------------------------------------
} -----------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} } To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} } On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
}
} } -------------------------------------------------------------
} ----------.
} }
} }
} } I recently completed a research paper to complete my EE
} } Degree which included a section on vacuum principles. I
} } incorporated part of the in formation I got from an article
} } by XEI scientific on contamination control. It spoke of oil
} } deposits on cool windows or a loss of low energy xray. I
} } thought I also read (on the web site) of how x-rays will
} } turn ceramics yellow. I can not find the article again.
} } Can you help me with this? What I am trying to decipher is,
} } will concentrated xray emission through a ceramic cause it
} } to yellow and could contamination deposits be the cause of
} } this? Would appreciate any answers or leads as to where I
} } might find the answer.
} }
} } Sincerely
} } Joe Metzger
} }
} }
} }
} }
}
}



From daemon Mon Apr 24 18:23:45 2000



From: Lesley S. Bechtold :      lsb-at-jax.org
Date: Mon, 24 Apr 2000 14:59:22 -0400
Subject: Immunogold labeling of platelets

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

I'll be attempting to do some post-embedding immunogold labeling of murine
platelets. I'm soliciting advice on preferred fixatives and resins. I'd
like to stay away from cryosectioning if possible. If anyone has a great
technique or special tips, I'd like to hear about it.

Thanks in advance!


Lesley S. Bechtold
Supervisor, Biological Imaging
The Jackson Laboratory
600 Main St.
Bar Harbor, ME 04609
207-288-6191



From daemon Mon Apr 24 18:23:45 2000



From: Bill Miller :      microbill-at-mohawk.net
Date: Mon, 24 Apr 2000 14:56:47 -0400
Subject: Re: digital cameras for copy stand work?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Fuji just came out with a new 4.3million pixel camera (Fujifilm FinePix
4700) utilizing their new octagonal pixel technology (Super CCD) and it
sells for {$1000. I saw it in a catalog from Publishing Perfection
(http:/www.publishingperfection.com)

At 11:01 AM 4/24/00 -0500, Beth Richardson wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Mon Apr 24 18:23:45 2000



From: Ken Bart :      kbart-at-hamilton.edu
Date: Mon, 24 Apr 2000 15:16:06 -0700
Subject: Re: digital cameras for copy stand work?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Beth:
There is an excellent review of the Nikon 990 at
http://www.dpreview.com/reviews/nikoncp990/. Maybe this addresses
your question. I suspect that this camera would be somewhat limited
for copy stand work. I do a lot of digital imaging in my lab and use
a Nikon D1, but this is considerably more expensive then a Coolpix
990. A good alternative is the Leaf Lumina which is marketed by
Electron Microscopy Sciences and others. I hope this helps!!!!!!

Ken Bart

Kenneth M. Bart
Director, Electron Microscopy Facility
Hamilton College
Clinton, New York 13323
----------------------------------------
Phone:315-859-4715
Fax: 315-859-4807
email: kbart-at-hamilton.edu



From daemon Mon Apr 24 18:23:46 2000



From: Barbara Foster :      mme-at-map.com
Date: Mon, 24 Apr 2000 16:18:16 -0400
Subject: MME Research

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


To Product, Sales, and Marketing managers,

MME's Market Research survey has been extended one day in honor of Friday's
holiday. If you have not returned your survey forms, please do so by end
of business Tuesday.

If, for some reason, we missed you, please email me immediately with your
fax number.

We already have nearly 10% return. Both a summary of our findings and the
winner of the M&M '99 Master Report will be posted Wednesday on
www.MicroscopyMarket.com

Thanks for your participation.

Barbara Foster,President
Microscopy/Marketing & Education
125 Paridon Street Suite 102
Springfield, MA 01118-2130
PH: 413-746-6931 FX: 413-746-9311 email: MME-at-map.com
Website: www.MME-Microscopy.com/marketing

MME is a full service technical marketing company specializing in
microscopy and related imaging technologies.
Catalytic information and more .... We help your company grow!
%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%



From daemon Mon Apr 24 18:23:47 2000



From: Dr. Raj Lartius :      rlartius-at-novascan.com
Date: Mon, 24 Apr 2000 15:34:08 -0500
Subject: RE: Quartz coverslips purity

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello

GE does sell quartz glass in the US. I don't recall seeing coverslips, but
we have purchase quartz that was approximately 1 mm in thickness. Here is
their contact information and a
website.

4901 Campbell Road
Willoughby, Ohio 44094 USA
Phone: (216) 266-3590 or 1-800-438-2100
FAX: (216) 266-4043 or 1-800-258-3803

http://www.gespectrum.com/inet/quartz/english/
Email: quartz-at-lighting.ge.com


Raj

**********************************************
Dr. Raj Lartius, CEO
NovaScan Technologies
Iowa State University Research Park
2501 North Loop Drive
Ames, Iowa USA 50010

Email: rlartius-at-novascan.com
Voice: 515-795-3164
Fax: 515-795-4414
Web: www.novascan.com
**********************************************
"Innovative Tools to Explore the Microworld"



From daemon Mon Apr 24 18:23:49 2000



From: Doug Cromey :      doug-cromey-at-ns.arizona.edu
Date: Mon, 24 Apr 2000 14:53:55 -0700
Subject: General Microscopy book recommendation?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Colleagues,

I was asked today if there exists a book that broadly covers all of the
different types of Microscopy (LM, EM, Probe Microscopes, etc) and gives
their pros & cons and perhaps examples of their use. The faculty member
who made the request is not a trained microscopist, so they would prefer
something more general.

If there is such a book, it sounds like it would be an interesting one to read.

Yours,
Doug Cromey
....................................................................
: Douglas W. Cromey, M.S. Dept. of Cell Biology & Anatomy :
: Research Specialist, Principal University of Arizona :
: (office: AHSC 4212A) P.O. Box 245044 :
: (voice: 520-626-2824) Tucson, AZ 85724-5044 USA :
: (FAX: 520-626-2097) (email: doug-cromey-at-ns.arizona.edu):
:...................................................................:
http://www.pharmacy.arizona.edu/exp_path.html
Home of: "Microscopy and Imaging Resources on the WWW"



From daemon Mon Apr 24 18:23:49 2000



From: William Snell :      william.snell-at-email.swmed.edu
Date: Mon, 24 Apr 2000 16:53:38 -0500
Subject: LM: looking for used Zeiss DR-C stereomicroscope or equivalent

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am looking for a stereomicroscope with Greenough optics that provides
final magnification of ~10X to 64X or 80X. I want to use it for
visualizing algal cells growing on agar petri dishes. The cells are
about 5 microns in diameter and I want to push them around on the agar
using fine glass needles. I understand that the old Zeiss DR-C works
well for this application. Vermont Optech and Sciscope have been very
helpful and may have usable, used systems. Any other suggestions/ideas
are welcome.

William J. Snell, Ph.D.
University of Texas Southwestern Medical Center
T-214-648-2332
F-214-648-8694
email-William.Snell-at-email.swmed.edu




From daemon Tue Apr 25 22:54:32 2000



From: De Pauw Bart :      bart.depauw-at-rug.ac.be
Date: Tue, 25 Apr 2000 15:32:15 +0200
Subject: Holder Filter

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Listers,

I am searching some device for holding (polycarbonate)filters during
dehydration and CPD. I am trying to view mycoplasms with SEM. Has anyone
experience with this ?

Greetings,


De Pauw Bart
Ghent University
Faculty of Veterinary Medicine
Morphology
Salisburylaan 133
9820 Merelbeke
Belgium
Phone : 0032(0)9 264.77.19
Fax : 0032(0)9 264.77.90



From daemon Tue Apr 25 22:54:33 2000



From: spb :      spb-at-mwrn.com
Date: Tue, 25 Apr 2000 09:28:18 -0400
Subject: Help locating Live Cell Analysis in Calif or AZ

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


If you can help with the message below, please respond to
BarbaraRBC-at-aol.com [mailto:BarbaraRBC-at-aol.com]

Please do NOT respond to the list

Susanne P Brandom
MicroWorld


MESSAGE

Can you assist by providing a list of live cell blood testers in the
California/Arizona area? If not, are you able to point me in the direction
where I might find a list of microscopists who perform this type of test?

Thank you

Barbara Churchill
(909) 624-2459 ( fax and voice mail)



From daemon Tue Apr 25 22:54:33 2000



From: George Laing :      scisales-at-ngscorp.com
Date: Tue, 25 Apr 2000 09:26:47 -0400
Subject: RE: digital cameras for copy stand work?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} From: Bill Miller [mailto:microbill-at-mohawk.net]
} Sent: Monday, April 24, 2000 2:57 PM
} To: Beth Richardson; microscopy-at-sparc5.microscopy.com
} Subject: Re: digital cameras for copy stand work?

} Fuji just came out with a new 4.3million pixel camera (Fujifilm FinePix
} 4700) utilizing their new octagonal pixel technology (Super CCD) and it
} sells for {$1000. I saw it in a catalog from Publishing Perfection }


Fuji has two new cameras coming out based on their new "Super CCD"
technology, the Finepix 4700 and the S-1Pro SLR Camera. In scientific
imaging, an important issue is image interpolation and you must be aware of
how Fuji determines resolution of these new cameras.
The Super CCD technology utilizes octagonal pixels which are aligned
at an angle, as opposed to horizontal rows for a conventional CCD with
square
pixels. The advantages of this design include denser packing of pixels, and
having pixels sensitive to R,G,B in each row. It is a very interesting
technology
to say the least.
Look closely at any Fuji literature about these cameras. The Finepix 4700
has an IMAGE resolution of 4.3 million pixels, the S1 Pro has an IMAGE
resolution
of 6 million pixels. Note this is not CCD resolution. The 4700 has a CCD
resolution
of approximately 2.3 million pixels, the S1 Pro has a CCD resolution of 3.2
million
pixels.
Fuji's image size specs are based on their claim that the image quality
with the
S1 Pro will be equivalent to an image captured with a 6 million pixel
"conventional" CCD
camera, or a 4.3 million pixel conventional CCD camera with the 4700.
I have no doubt that the new cameras will produce high quality images. I
can
also say for sure that the cost of the new cameras is very affordable when
compared to
similar products currently available. It remains to be seen what effects
that Fuji's
interpolation will have on image integrity.
S-1 Cameras are expected in late May or early June, I will be happy to
provide
sample images to any who request them at that time. Finepix 4700 cameras are
currently
starting to ship from Fuji.

George Laing
National Graphic Supply
scisales-at-ngscorp.com
(800) 223-7130 X3109



From daemon Tue Apr 25 22:54:34 2000



From: Barbara Foster :      mme-at-map.com
Date: Tue, 25 Apr 2000 10:06:47 -0400
Subject: Re: General Microscopy book recommendation?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Doug,

Our book, "Optimizing Light Microscopy" covers the light end of things and
there is a short chapter covering EM, confocal, etc. Details are on our
website: MME-Microscopy.com/education. A number of colleges and
universities have started using it as a text, most recently, U Wash (Kip
Hauch).

Caveat: MME does have a financial interest in this project.

Best regards
Barbara Foster,President
Microscopy/Microscopy Education
125 Paridon Street Suite 102
Springfield, MA 01118-2130
PH: 413-746-6931 FX: 413-746-9311 email: MME-at-map.com
Website: www.MME-Microscopy.com/education

America's first national consortium of microscopy specialists offering
customized on-site training in all areas of microscopy, image analysis, and
sample preparation
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^


At 02:53 PM 4/24/00 -0700, Doug Cromey wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Tue Apr 25 22:54:36 2000



From: Lawrence Kordon :      nikon-at-jagunet.com
Date: Tue, 25 Apr 2000 10:33:24 -0400
Subject: Re: digital cameras for copy stand work?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thanks George!

I was tempted to write a similiar response, but it would seem too biased. However, I
just read a recent Wall Street Journal article that stated that Olympus Corporation
has filed a law suit against Fuji. Appearantly, Fuji does not feel they have a strong
defense as they are instructing Dealers to place a correction sticker over the
resolution specifications on the boxes and make disclaimers in all advertisements.

Regards,

Lawrence Kordon
Nikon, Inc.
nikon-at-jagunet.com

George Laing wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} } From: Bill Miller [mailto:microbill-at-mohawk.net]
} } Sent: Monday, April 24, 2000 2:57 PM
} } To: Beth Richardson; microscopy-at-sparc5.microscopy.com
} } Subject: Re: digital cameras for copy stand work?
}
} } Fuji just came out with a new 4.3million pixel camera (Fujifilm FinePix
} } 4700) utilizing their new octagonal pixel technology (Super CCD) and it
} } sells for {$1000. I saw it in a catalog from Publishing Perfection }
}
} Fuji has two new cameras coming out based on their new "Super CCD"
} technology, the Finepix 4700 and the S-1Pro SLR Camera. In scientific
} imaging, an important issue is image interpolation and you must be aware of
} how Fuji determines resolution of these new cameras.
} The Super CCD technology utilizes octagonal pixels which are aligned
} at an angle, as opposed to horizontal rows for a conventional CCD with
} square
} pixels. The advantages of this design include denser packing of pixels, and
} having pixels sensitive to R,G,B in each row. It is a very interesting
} technology
} to say the least.
} Look closely at any Fuji literature about these cameras. The Finepix 4700
} has an IMAGE resolution of 4.3 million pixels, the S1 Pro has an IMAGE
} resolution
} of 6 million pixels. Note this is not CCD resolution. The 4700 has a CCD
} resolution
} of approximately 2.3 million pixels, the S1 Pro has a CCD resolution of 3.2
} million
} pixels.
} Fuji's image size specs are based on their claim that the image quality
} with the
} S1 Pro will be equivalent to an image captured with a 6 million pixel
} "conventional" CCD
} camera, or a 4.3 million pixel conventional CCD camera with the 4700.
} I have no doubt that the new cameras will produce high quality images. I
} can
} also say for sure that the cost of the new cameras is very affordable when
} compared to
} similar products currently available. It remains to be seen what effects
} that Fuji's
} interpolation will have on image integrity.
} S-1 Cameras are expected in late May or early June, I will be happy to
} provide
} sample images to any who request them at that time. Finepix 4700 cameras are
} currently
} starting to ship from Fuji.
}
} George Laing
} National Graphic Supply
} scisales-at-ngscorp.com
} (800) 223-7130 X3109



From daemon Tue Apr 25 22:54:37 2000



From: RCHIOVETTI-at-aol.com
Date: Tue, 25 Apr 2000 11:48:19 EDT
Subject: Re: kodak digital camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In a message dated 04/24/2000 10:32:32 AM US Mountain Standard Time,
RonMervis-at-aol.com-at-sparc5.microscopy.com writes:

{ { we are considering getting a kodak MDS 120 digital camera for
photomicroscopy
(brightfield)...does anyone have any experience with this system...is it
cost
effective....? user friendly?
} }

Hi Ron,

The Kodak MDS 120 seems to do a decent job. Part of the MDS (Microscopy
Documentation System) with the camera is a c-mount adapter that you mount to
the phototube of your microscope. It requires a 1.0X adapter in the
phototube, and the adapter should have a c-mount on it. The Kodak adapter
then mounts on the end of the 1.0X adapter.

You may encounter some vignetting with the camera, especially if you try to
use the wide-angle setting on the camera's zoom lens. The vignetting seems
to be a function of the photo adapters which are used, and Kodak has a
disclaimer about this in the instruction manual. It may or may not be a
problem with your scope/phototube/c-mount adapter.

Part of the package which we got is a 16 MB Flash Memory card for the camera,
so you can either run the camera from your computer or store images on the
memory card and then remove the card and take it to a computer to transfer
the images to the computer. The package also had a PCMCIA card adapter in
it. The memory card plugs into the end of the PCMCIA device, and you can
then slip it into a PCMCIA slot on a laptop computer.

But if you need to use a desktop computer and don't have a PCMCIA slot, you
need to get something like a SanDisk card reader for the computer. They're
available at CompUSA, Fry's Electronics, OfficeMax, etc. for about $70.

The software with the camera is very nice. It lets you transfer images from
the camera's memory directly or from from the memory card, preview images and
download them directly from the camera, etc. as well as perform some basic
image adjustments (flip, rotate, modify brightness, contrast, gamma, etc.)

Overall, I would say the Kodak system is a nice way to document general
microscopy images in brightfield and phase contrast microscopy for under $2K.
We haven't tried to use the camera for fluorescence imaging, so I have no
history to contribute on this subject.

Hope this is of some help!

Bob Chiovetti
GTI Microsystems



From daemon Tue Apr 25 22:54:41 2000



From: Vladimir Oleshko :      Vladimir.Oleshko-at-asu.edu
Date: Mon, 24 Apr 2000 22:35:35 -0700
Subject: General Microscopy book recommendation?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


There is the Handbook of Microscopy: Applications in Materials Science,
Solid State Physics and Chemistry /ed by S. Amelinckx et al., v.1-3,
Weinheim, N.Y.: VCH (1997). It covers LM, EM (stationary and scanning beam
methods), X-ray and acoustic microscopy, scanning probe techniques, magnetic
and emission microscopies, image analysis, and microscopy applications to
various classes of materials as well.

Vladimir

************************************
Vladimir P. Oleshko, Ph.D.
Industrial Associates Program
Center for Solid State Science
Arizona State University
Main Campus, PO Box 871704
Tempe, AZ 85287-1704
(480) 727-7666
Fax: (480) 965-9004
E-Mail:oleshko-at-imap3.asu.edu
*************************************


-----Original Message-----
} From: Doug Cromey [mailto:doug-cromey-at-ns.arizona.edu]
Sent: Monday, April 24, 2000 2:54 PM
To: microscopy-at-sparc5.microscopy.com


Colleagues,

I was asked today if there exists a book that broadly covers all of the
different types of Microscopy (LM, EM, Probe Microscopes, etc) and gives
their pros & cons and perhaps examples of their use. The faculty member
who made the request is not a trained microscopist, so they would prefer
something more general.

If there is such a book, it sounds like it would be an interesting one to
read.

Yours,
Doug Cromey
...................................................................
: Douglas W. Cromey, M.S. Dept. of Cell Biology & Anatomy :
: Research Specialist, Principal University of Arizona :
: (office: AHSC 4212A) P.O. Box 245044 :
: (voice: 520-626-2824) Tucson, AZ 85724-5044 USA :
: (FAX: 520-626-2097) (email: doug-cromey-at-ns.arizona.edu):
:...................................................................:
http://www.pharmacy.arizona.edu/exp_path.html
Home of: "Microscopy and Imaging Resources on the WWW"




From daemon Tue Apr 25 22:54:50 2000



From: Shane Collins :      kshanec-at-gte.net
Date: Tue, 25 Apr 2000 15:59:26 -0700
Subject: RE: digital cameras for copy stand work?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In addition to what George and Bill have pointed out, Fuji claims the imager
(ccd) is roughly the size of APS film on the S1 Pro. What I cannot find in
the literature is the pixel size specification whereby one can calculate or
compare the field of view (on a microscope) of this camera with other mega
pixel cameras.
Shane

K. Shane Collins
Scientific Instrument Company
805.444.4953 cell
310.568.9188 office
310.568.9189 fax


-----Original Message-----
} From: George Laing [mailto:scisales-at-ngscorp.com]
Sent: Tuesday, April 25, 2000 6:27 AM
To: Microscopy-at-sparc5.microscopy.com


} From: Bill Miller [mailto:microbill-at-mohawk.net]
} Sent: Monday, April 24, 2000 2:57 PM
} To: Beth Richardson; microscopy-at-sparc5.microscopy.com
} Subject: Re: digital cameras for copy stand work?

} Fuji just came out with a new 4.3million pixel camera (Fujifilm FinePix
} 4700) utilizing their new octagonal pixel technology (Super CCD) and it
} sells for {$1000. I saw it in a catalog from Publishing Perfection }


Fuji has two new cameras coming out based on their new "Super CCD"
technology, the Finepix 4700 and the S-1Pro SLR Camera. In scientific
imaging, an important issue is image interpolation and you must be aware of
how Fuji determines resolution of these new cameras.
The Super CCD technology utilizes octagonal pixels which are aligned
at an angle, as opposed to horizontal rows for a conventional CCD with
square
pixels. The advantages of this design include denser packing of pixels, and
having pixels sensitive to R,G,B in each row. It is a very interesting
technology
to say the least.
Look closely at any Fuji literature about these cameras. The Finepix 4700
has an IMAGE resolution of 4.3 million pixels, the S1 Pro has an IMAGE
resolution
of 6 million pixels. Note this is not CCD resolution. The 4700 has a CCD
resolution
of approximately 2.3 million pixels, the S1 Pro has a CCD resolution of 3.2
million
pixels.
Fuji's image size specs are based on their claim that the image quality
with the
S1 Pro will be equivalent to an image captured with a 6 million pixel
"conventional" CCD
camera, or a 4.3 million pixel conventional CCD camera with the 4700.
I have no doubt that the new cameras will produce high quality images. I
can
also say for sure that the cost of the new cameras is very affordable when
compared to
similar products currently available. It remains to be seen what effects
that Fuji's
interpolation will have on image integrity.
S-1 Cameras are expected in late May or early June, I will be happy to
provide
sample images to any who request them at that time. Finepix 4700 cameras are
currently
starting to ship from Fuji.

George Laing
National Graphic Supply
scisales-at-ngscorp.com
(800) 223-7130 X3109




From daemon Tue Apr 25 22:54:51 2000



From: bozzola-at-siu.edu
Date: Tue, 25 Apr 2000 18:48:13 -0500
Subject: Microscopy Footage Needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Microscopists:

I am forwarding this request in the hopes that someone would be able
to assist these film producers to find some footage for a museum
exhibit they are working on.

Thanks for our attention.

John Bozzola

+++++++++++++++++++++++++++++++++++++++++++++++++

Chedd-Angier is producing a series of exhibits for the Science Museum of
Virginia in Richmond, VA.(. http://www.chedd-angier.com.)
We currently produce the Scientific American Frontiers Program on
PBS.(http://www.pbs.org/saf)

Our science museum project encompasses a wide variety of exhibits
ranging from a voyage through a cell called cell watcher, to a life size
journey through the human body called BODY PROBE.
I am looking for a variety of footage pieces to use in these exhibits.
They will all be used for North American non broadcast educational use
only in a museum that DOES not have a separate admission policy for
this exhibit.
The electron micrograph footage that I am looking for is :

For cell watcher
Mitochondria
Golgi Bodies
Cell membranes
Nucleus
Chromosomes
Vesicles
Endoplasmic Reticulum
Lysosomes
Fat cells expanding
Mitosis
White blood cells
muscle cells contracting
plant stem cells elongating
cells from a fallopian tube
amoeba
sperm cells swimming
This is a very extensive list, and if there are animation related
footage sources I can use those as well.
If you have any questions at all please contact me at 617-926-8300.
Thank you

AndrŽ Stark
Producer
Chedd-Angier
70 Coolidge Hill Rd
Watertown, MA 02472
0101617-926-8300
617-926-2710(F)

+++++++++++++++++++++++++++++++++++++++++++++++



From daemon Tue Apr 25 22:54:57 2000



From: Hazel Clothier :      HazelC-at-spc.int
Date: Wed, 26 Apr 2000 12:55:16 +1100
Subject: LM basic maintenance manuals

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


As a new subscriber to the listserv I would like to ask your assistance in
locating copies of any manuals or in-house guidelines for the basic care and
maintenance of light microscopes.

I am currently assisting a group of microscopists in a humid / tropical
environment to increase their ability to care for their microscopes. Care by
the user is an important issue as is the ability to repair/maintain
microscopes in-country. Whilst we can find quite a bit of information on how
to use your microscope there is less on maintenance.

In addition does anyone have any idea on where we can purchase a "two-pin
tool" for removing lenses?

Thankyou

Hazel Clothier

Regional Laboratory Scientist
Pacific Regional Vector Borne Diseases Project
Secretariat of the Pacific Community
PMB
Suva
FIJI

Tel: (679) 321154 - direct
(679) 320066 - ex 110
Fax:(679) 322714
hazelc-at-int



From daemon Tue Apr 25 23:02:11 2000



From: Aaron Wheeler :      adog3050-at-yahoo.com
Date: Tue, 25 Apr 2000 22:56:46 -0500
Subject: TEM: cacodylate vs phosphate buffer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi:

I am a beginning microscopist. I am preparing to take
some TEM photos of granules isolated from mast cells.
The technician I am working with would like to use
phosphate buffer (+ glutaraldehyde) to fix the
granules, but the literature I've read suggests using
cacodylate buffer. What do you think about the
benefits/disadvantages of phosphate vs cacodylate
buffer for these purposes?

thanks,
Aaron

__________________________________________________
Do You Yahoo!?
Send online invitations with Yahoo! Invites.
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From daemon Wed Apr 26 06:29:45 2000



From: k.denhaan-at-consunet.nl
Date: Tue, 25 Apr 2000 23:01:20 -0500
Subject: phase contrast vs interference contrast

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Email: k.denhaan-at-consunet.nl
Name: K.den Haan
School: dutch HBS

Question: when comparing phase contrast and interference
contrast (Nomarski) the negative points a.o.(Ph)
are halo. Interference contrast also
(especially in minute organisms) has a drawback
since depending on the position of the polarizer
some part of f.i.bacteria do not show the
relief image (the shadow) to its fullest. How
do you call this phenomenon and how can it be
explained?




---------------------------------------------------------------------------




From daemon Wed Apr 26 06:29:45 2000



From: COURYHOUSE-at-aol.com
Date: Wed, 26 Apr 2000 00:02:52 EDT
Subject: Re: General Microscopy book recommendation?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


if anyone has a used set of this avail let me know. also looking for
encyclopedia of microscopy by gray (editor)
thanks Ed Sharpe

{ { Subj: RE: General Microscopy book recommendation?
Date: 4/25/00 2:29:52 PM US Mountain Standard Time
From: Vladimir.Oleshko-at-asu.edu (Vladimir Oleshko)
Reply-to: {A HREF="mailto:oleshko-at-imap3.asu.edu"} oleshko-at-imap3.asu.edu {/A}
To: doug-cromey-at-ns.arizona.edu ('Doug Cromey')
CC: microscopy-at-sparc5.microscopy.com

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America
To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
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-----------------------------------------------------------------------.


There is the Handbook of Microscopy: Applications in Materials Science,
Solid State Physics and Chemistry /ed by S. Amelinckx et al., v.1-3,
Weinheim, N.Y.: VCH (1997). It covers LM, EM (stationary and scanning beam
methods), X-ray and acoustic microscopy, scanning probe techniques, magnetic
and emission microscopies, image analysis, and microscopy applications to
various classes of materials as well.

Vladimir

************************************
Vladimir P. Oleshko, Ph.D.
Industrial Associates Program
Center for Solid State Science
Arizona State University
Main Campus, PO Box 871704
Tempe, AZ 85287-1704
(480) 727-7666
Fax: (480) 965-9004
E-Mail:oleshko-at-imap3.asu.edu
*************************************

} }



From daemon Wed Apr 26 06:32:08 2000



From: Lam xu Fu Christopher :      eng81067-at-nus.edu.sg
Date: Wed, 26 Apr 2000 15:13:14 +0800
Subject: SEM charging effects

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Sir,

I am currently using a JEOL 5800LV SEM.
My specimens are cells from cultures which we have grown in our labs.
The low vacuum condition is 7Pa.
The problem occurs when I increase the magnification, about 400 times and
beyond. The picture I get will be enlarged and clear except for certain
regions which displays some glaring effects. Playing around with the
contrasts and brightness didn't help.
I was told this was a charging problem, and increasing the vacuum might
help, so I tried. It did help a little but the picture quality was
compromised.

Are there other solutions to this problem of mine, such that the picture
quality might remain the same.

Chris Lam
BIOMAT
Mechanical & Production Department
National University of Singapore




From daemon Wed Apr 26 06:32:09 2000



From: Jeremy Sanderson :      jb_sanderson-at-yahoo.com
Date: Wed, 26 Apr 2000 01:08:59 -0700 (PDT)
Subject: Books for Microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Following the thread from Doug Cromey asking about
books for Microscopy, I would like to suggest two
manuals for light microscopy that are comprehensive in
their coverage, *accurate in their educational
content*, and easy to read and digest.

Title: Light Microscopy, An Illustrated Guide.
Author: Ron OLDFIELD.
Location: Sydney, Australia
Published: 1994, Wolfe Publishing, imprint of
Mosby-Year Book, Europe. 160 pages
ISBN: 0-7234-1876-4
Available Amazon, no price given; ca. $20.

Title: Introduction to Light Microscopy
Authors: Savile BRADBURY & Brian BRACEGIRDLE
Location: Oxford, England
Published: 1998, Bios Scientific Publishers
Website: www.bios.co.uk
Royal Microscopical Society Handbook No. 42, 122
pages.
ISBN: 1-85996-121-5
Amazon Price: $32.95

Searching for, and finding, a suitable teaching and/or
reference text is a personal thing. It depends on your
requirements and preferences, but I suggest that these
two books will help most people in most disciplines. I
have used them for the last six years in teaching
light microscopy to a wide spectrum of students from
industry and academia.

For those intessted in digital imaging, although not a
book specifically for microscopists, I have found the
following text useful:

Title: Digital Imaging for Photographers, 3rd edn.
Authors: Adrain DAVIES & Phil FENNESSY
Published: 1998, Focal Press, Oxford, Boston.170
pages.
website: www.bh.com/focalpress
(Focal Press is an imprint of Butterworth-Heinemann)
ISBN: 0-240-51538-2
Amazon Price: $31.96

I am both a professional and an amateur microscopist,
please do not think I am denigrating the word amateur,
but as an introductory text for amateur
microscopists,or those starting out in a complex
field, I would suggest:

Title: Exploring With the Microscope : A Book of
Discovery & Learning
Author: Werner NACHTIGALL
Publisher: Stirling Publishing Corp. Inc., April 1997.
160 pages
ISBN: 0-80690866-1 (check that this is 2nd edition)
Amazon Price: $11.96

Finally, if you consider that getting school children
interested in microscopy, and science generally, is an
important investment for the future, then I would
suggest the new Usborne Complete Book of the
Microscope, which shows the relevance and the
application of microscopy in many walks of life.

Title: The Usborne Complete Book of the Microscope
Author: Kirsteen ROGERS
Publisher: 1998, Usborne Publishing Ltd, 96 pages.
website: www.usborne.com
ISBN: 0-7460-3106-8
Amazon Price: $22.95

Whatever your field, light microscopy underpins, and
is a necessary foundation for, electron microscopy,
confocal and other techniques. I hope that these
suggestions are useful. I would welcome anyone's views
to me directly.

Jeremy Sanderson
jb_sanderson-at-yahoo.com


__________________________________________________
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Send online invitations with Yahoo! Invites.
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From daemon Wed Apr 26 06:32:09 2000



From: Petra Wahlbring :      wahlbrin-at-crpgl.lu
Date: Wed, 26 Apr 2000 10:55:50 +0200
Subject: TEM: permanganate etching

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear colleagues,

I have a few PE specimens on my desk that I want to prepare with the
"permanganate etching method". Does anybody of you have some experience on
this techique? Would you share some details or tips and tricks with me on
how to obtain the best results? What is the best method to produce a
replica from the etched specimen?

Petra
--------------------------------------------------------------
Dr. Petra Wahlbring
Centre de Recherche Public - Gabriel Lippmann
Laboratoire d'Analyse des Materiaux (LAM)
162a, av. de la Faiencerie L-1511 Luxembourg
tel. +352-466644-402 fax +352-466644-400
e-mail: petra.wahlbring-at-crpgl.lu
Visit our WWW site! http://www.crpgl.lu/~wahlbrin



From daemon Wed Apr 26 06:32:10 2000



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Wed, 26 Apr 2000 11:21:30 +0100 (GMT Daylight Time)
Subject: Re: TEM: cacodylate vs phosphate buffer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Phosphate buffers can give rise to a precipitate with
uranyl acetate if it is used as an en block stain.
Cacodylate does not.

Cacodylate contains arsenic and must be used with care.

Dave


On Tue, 25 Apr 2000 22:56:46 -0500 Aaron Wheeler
{adog3050-at-yahoo.com} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi:
}
} I am a beginning microscopist. I am preparing to take
} some TEM photos of granules isolated from mast cells.
} The technician I am working with would like to use
} phosphate buffer (+ glutaraldehyde) to fix the
} granules, but the literature I've read suggests using
} cacodylate buffer. What do you think about the
} benefits/disadvantages of phosphate vs cacodylate
} buffer for these purposes?
}
} thanks,
} Aaron
}
} __________________________________________________
} Do You Yahoo!?
} Send online invitations with Yahoo! Invites.
} http://invites.yahoo.com
}
}
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"



From daemon Wed Apr 26 06:46:22 2000



From: Duncan Waddell :      D.Waddell-at-mailbox.uq.edu.au
Date: Wed, 26 Apr 2000 06:33:26 -0500
Subject: EM Messageboard

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi folks,

The CMM at The University of Queensland (Australia) has initiated an
Internet messageboard for Microscopy and Microanalysis.

http://www.coolboard.com/boardshow.cfm?mb=545625259139077

Other EM websites around the world are invited to add this forum to
their own sites - just follow the link on the Forum to customize your
own version for your own web pages.

The service is hosted by Coolboard and is totally free.

It is currently being moderated ONLY for the purpose of keeping rubbish
and spam out of it. If all goes well moderation will be dropped.

Check it out and participate in this EM community venture if you feel it
will be of benefit.

Regards,

Duncan.

--
************************************************************
Duncan Waddell (BSc)
Senior Scientific Officer
Centre for Microscopy and Microanalysis
The University of Queensland, St. Lucia, Qld, 4072 Australia
Telephone: +61-7-3365-4216
Facsimile: +61-7-3365-4422
WWW: http://www.uq.edu.au/nanoworld/nanohome.html
************************************************************
Any opinion expressed is that of the writer,
and not necessarily that of CMM or of the University.
************************************************************




From daemon Wed Apr 26 06:59:44 2000



From: George Laing :      scisales-at-ngscorp.com
Date: Tue, 25 Apr 2000 21:48:32 -0400
Subject: RE: Cameras for Copy Stand use

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


FYI all,

A review of the Fuji Finepix 4700 is available at:
http://www.dpreview.com/reviews/fuji4700z/

A review of the Nikon Coolpix 990 is available at:
http://www.imaging-resource.com/PRODS/C990/C99A.HTM

If anyone would like literature, or sample prints/ files from the Nikon
please contact me directly.

George Laing

National Graphic Supply
226 North Allen Street
Albany, NY 12206
E-mail: scisales-at-ngscorp.com
(800) 223-7130 X3109 Phone
(800) 832-2205 Fax





From daemon Thu Apr 27 11:02:14 2000



From: jim :      jim-at-proscitech.com.au
Date: Wed, 26 Apr 2000 22:44:05 +1000
Subject: RE: digital cameras for copy stand work?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Shane Collins - do you mean pixel size and numbers when you refer to field of
view?
Field of view, as I understand it has nothing to do with image quality, but is
simply the physical size covered by a photographic system on the microscope.
The crucial factors affecting field of view are objective lens mag. and the
relation between photo eyepiece and the physical size of the film or CCD. 35mm
film combined with a 3x photo eyepiece is about "normal", small CCD's require a
much lower mag eyepiece, otherwise the system gives too greater magnifications,
frequently beyond OM limits.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Wednesday, April 26, 2000 8:59 AM, Shane Collins [SMTP:kshanec-at-gte.net]
wrote:
}
}
} In addition to what George and Bill have pointed out, Fuji claims the imager
} (ccd) is roughly the size of APS film on the S1 Pro. What I cannot find in
} the literature is the pixel size specification whereby one can calculate or
} compare the field of view (on a microscope) of this camera with other mega
} pixel cameras.
} Shane
}
} K. Shane Collins
} Scientific Instrument Company
} 805.444.4953 cell
} 310.568.9188 office
} 310.568.9189 fax
}
}
} -----Original Message-----
} } From: George Laing [mailto:scisales-at-ngscorp.com]
} Sent: Tuesday, April 25, 2000 6:27 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: RE: digital cameras for copy stand work?
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} } From: Bill Miller [mailto:microbill-at-mohawk.net]
} } Sent: Monday, April 24, 2000 2:57 PM
} } To: Beth Richardson; microscopy-at-sparc5.microscopy.com
} } Subject: Re: digital cameras for copy stand work?
}
} } Fuji just came out with a new 4.3million pixel camera (Fujifilm FinePix
} } 4700) utilizing their new octagonal pixel technology (Super CCD) and it
} } sells for {$1000. I saw it in a catalog from Publishing Perfection }
}
}
} Fuji has two new cameras coming out based on their new "Super CCD"
} technology, the Finepix 4700 and the S-1Pro SLR Camera. In scientific
} imaging, an important issue is image interpolation and you must be aware of
} how Fuji determines resolution of these new cameras.
} The Super CCD technology utilizes octagonal pixels which are aligned
} at an angle, as opposed to horizontal rows for a conventional CCD with
} square
} pixels. The advantages of this design include denser packing of pixels, and
} having pixels sensitive to R,G,B in each row. It is a very interesting
} technology
} to say the least.
} Look closely at any Fuji literature about these cameras. The Finepix 4700
} has an IMAGE resolution of 4.3 million pixels, the S1 Pro has an IMAGE
} resolution
} of 6 million pixels. Note this is not CCD resolution. The 4700 has a CCD
} resolution
} of approximately 2.3 million pixels, the S1 Pro has a CCD resolution of 3.2
} million
} pixels.
} Fuji's image size specs are based on their claim that the image quality
} with the
} S1 Pro will be equivalent to an image captured with a 6 million pixel
} "conventional" CCD
} camera, or a 4.3 million pixel conventional CCD camera with the 4700.
} I have no doubt that the new cameras will produce high quality images. I
} can
} also say for sure that the cost of the new cameras is very affordable when
} compared to
} similar products currently available. It remains to be seen what effects
} that Fuji's
} interpolation will have on image integrity.
} S-1 Cameras are expected in late May or early June, I will be happy to
} provide
} sample images to any who request them at that time. Finepix 4700 cameras are
} currently
} starting to ship from Fuji.
}
} George Laing
} National Graphic Supply
} scisales-at-ngscorp.com
} (800) 223-7130 X3109
}
}



From daemon Thu Apr 27 11:02:17 2000



From: Tom Phillips :      PhillipsT-at-missouri.edu
Date: Wed, 26 Apr 2000 09:39:26 -0500
Subject: Re: TEM: cacodylate vs phosphate buffer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


PO4 buffers can cause Calcium to precipitate out but the use of
cacodylate is archaic. Microscopists tend to be the most refractory
of all scientists to change. HEPES or PIPES are reasonable
alternatives for almost all procedures in which cacodylate is used -
certainly widely used for basic fixation such as you describe. they
are non-toxic, less expensive, and better buffers. good luck



} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)



From daemon Thu Apr 27 11:02:17 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Wed, 26 Apr 2000 08:33:27 -0700
Subject: RE: digital cameras for copy stand work?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The Fuji Super CCD is 23.3 x 15.6mm; 3040 x 2016 pixels.
The S1 Pro uses Nikon mount lenses and has an equivalent
35mm frame size at 1.5X the lens' focal length.

What the equivalent FOV would be on a scope is still in
question. If the S1 Pro works anywhere near how the
Nikon E1 or E2 does, I fear the Fuji camera won't be applicable
to microscopy at all. Without a lens, the camera must operate
in aperture priority mode and center weighted exposure
reading. Unless Fuji made some major changes to the basic
Nikon (N-60) body and associated electronics, they may have
just made a higher pixel count Nikon digicam.

gary g.


At 03:59 PM 4/25/00 , you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Thu Apr 27 11:02:18 2000



From: Robert H. Olley :      r.h.olley-at-reading.ac.uk
Date: Wed, 26 Apr 2000 16:55:34 +0100 (BST)
Subject: Re: TEM: permanganate etching

Contents Retrieved from Microscopy Listserver Archives
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Dear Petra,

Do you have the following paper to hand?

Refinement of Etching Techniques to Reveal Lamellar Profiles in
Polyethylene Banded Spherulites
Shahin,M.M., Olley,R.H., Blissett,M.J.
J. Polym. Sci. Polym. Part B: Polym. Phys. 1999, vol.37, pp. 2279-2286

This is our latest development in the technique. If you don't have a
copy, I can send you a reprint.

Generally, we use a two stage replication technique, making a first stage
replica out of cellulose acetate, then putting Ta/W shadow and carbon on
this and extracting the cellulose acetate.

If you can let me know a little bit more about your PE specimens, I can
give you a few more details specifically adapted to your type of specimen.
In particular, is it HDPE, LLDPE or LDPE?

+------------------------------------------------------------------------+
| Robert H.Olley Phone: |
| J.J.Thomson Physical Laboratory {direct line +44 (0) 118 9318572 |
| University of Reading {University internal extension 7867 |
| Whiteknights Fax +44 (0) 118 9750203 |
| Reading RG6 6AF Email: R.H.Olley-at-reading.ac.uk |
| England URL: http://www.reading.ac.uk/~spsolley |
+------------------------------------------------------------------------+




From daemon Thu Apr 27 11:02:22 2000



From: MICHAEL DELANNOY :      delannoy-at-mail.jhmi.edu
Date: Wed, 26 Apr 2000 14:22:02 -0400 (EDT)
Subject: Re: TEM: cacodylate vs phosphate buffer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Aron,
Caco is more extractive which may (or may not) be a blessing if
you are trying to clear out the cytoplasm a bit to get some more detail.
Poshpate is more physiologic but may form artifactual granules with
glutaraldehyde and osmium (in routine tandem use). I generally use
caco for all non immuno work otherwise phosphate, pbs or hepes for
IEM. Also remember caco contains arsenic. Good luck.

Mike Delannoy
JHMI Microscopy Facility




From daemon Thu Apr 27 11:02:23 2000



From: HILDEGARD CROWLEY :      hcrowley-at-du.edu
Date: Wed, 26 Apr 2000 12:52:56 -0600 (MDT)
Subject: Re: TEM: cacodylate vs phosphate buffer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




On Tue, 25 Apr 2000, Aaron Wheeler wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi:
}
} I am a beginning microscopist. I am preparing to take
} some TEM photos of granules isolated from mast cells.
} The technician I am working with would like to use
} phosphate buffer (+ glutaraldehyde) to fix the
} granules, but the literature I've read suggests using
} cacodylate buffer. What do you think about the
} benefits/disadvantages of phosphate vs cacodylate
} buffer for these purposes?
}
} thanks,
} Aaron
}
} __________________________________________________
} Do You Yahoo!?
} Send online invitations with Yahoo! Invites.
} http://invites.yahoo.com
}
}
}
}
Whenever possible (nearly all the time) use phosphate buffer. Cacodylate
contains arsenic which is a potent carcinogen. One should never get into
the habit of using arsenic buffer "just because". There must be a real
reason for it! Once cacodylate is in permanent use in a laboratory, dust
accumulates on the outside of bottles, on the inside of laboratory
glassware waiting to be washed, and so on. The effects are cumulative.
Arsenic is also a toxic to the kidneys, etc. Very dangerous stuff mostly
because it is in use in many laboratories day in and day out.

Hildy Crowley
University of Denver
Denver, CO



From daemon Thu Apr 27 11:02:23 2000



From: HILDEGARD CROWLEY :      hcrowley-at-du.edu
Date: Wed, 26 Apr 2000 12:55:12 -0600 (MDT)
Subject: Re: TEM: cacodylate vs phosphate buffer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




On Wed, 26 Apr 2000, Patton, David wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Phosphate buffers can give rise to a precipitate with
} uranyl acetate if it is used as an en block stain.
} Cacodylate does not.
}
} Cacodylate contains arsenic and must be used with care.
}
} Dave
}
}
} On Tue, 25 Apr 2000 22:56:46 -0500 Aaron Wheeler
} {adog3050-at-yahoo.com} wrote:
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Hi:
} }
} } I am a beginning microscopist. I am preparing to take
} } some TEM photos of granules isolated from mast cells.
} } The technician I am working with would like to use
} } phosphate buffer (+ glutaraldehyde) to fix the
} } granules, but the literature I've read suggests using
} } cacodylate buffer. What do you think about the
} } benefits/disadvantages of phosphate vs cacodylate
} } buffer for these purposes?
} }
} } thanks,
} } Aaron
} }
} } __________________________________________________
} } Do You Yahoo!?
} } Send online invitations with Yahoo! Invites.
} } http://invites.yahoo.com
} }
} }
} }
}
} ----------------------------------------
} Patton, David
} Email: David.Patton-at-uwe.ac.uk
} "University of the West of England"
}
}
}
If it is necessary to use UA with a buffer, use maleate systems. No
precipitate!

Hildy Crowley
University of Denver
Denver, CO



From daemon Thu Apr 27 11:02:24 2000



From: Roger Moretz :      rcmoretz-at-excite.com
Date: Wed, 26 Apr 2000 12:32:40 -0700 (PDT)
Subject: LM Ergonomic Stand

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Looking for the adjustable lucite bases made several years ago for adjusting
a LM for improved ergonomic access/use. I had the
information/supplier/manufacturer info around here someplace, but other than
the somewhat random neuronal firing, I appear to have
lost/misplace/misfiled/disposed of everything. I have a pathologist who
needs to incline his Nikon Microphot FX-A about 5 degrees. We have begun
the process of having a fixed wooden base constructed, but I much prefer the
lucite devices. Any help/ideas? TIA.


Roger C Moretz, Ph.D.





_______________________________________________________
Get 100% FREE Internet Access powered by Excite
Visit http://freelane.excite.com/freeisp



From daemon Thu Apr 27 11:02:24 2000



From: Roger Moretz :      rcmoretz-at-excite.com
Date: Wed, 26 Apr 2000 12:32:55 -0700 (PDT)
Subject: LM Ergonomic Stand

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Looking for the adjustable lucite bases made several years ago for adjusting
a LM for improved ergonomic access/use. I had the
information/supplier/manufacturer info around here someplace, but other than
the somewhat random neuronal firing, I appear to have
lost/misplace/misfiled/disposed of everything. I have a pathologist who
needs to incline his Nikon Microphot FX-A about 5 degrees. We have begun
the process of having a fixed wooden base constructed, but I much prefer the
lucite devices. Any help/ideas? TIA.


Roger C Moretz, Ph.D.





_______________________________________________________
Get 100% FREE Internet Access powered by Excite
Visit http://freelane.excite.com/freeisp



From daemon Thu Apr 27 11:02:25 2000



From: Roger Moretz :      rcmoretz-at-excite.com
Date: Wed, 26 Apr 2000 12:38:10 -0700 (PDT)
Subject: LM Ergonomic Stand

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Looking for the adjustable lucite stand marketed several years ago (at least
within this fuzzy brain of mine it was that long ago) for improving the
ergonomic interface to LMs. I have a pathologist who needs to incline his
Nikon Microphot FX-A about 5 degrees. We had just about decided to have a
fixed unit built by the carpentry shop, when I remembered the lucite
devices. Can anyone help point me in the right directions? TIA.


Roger C Moretz, Ph.D.
Dept of Toxicology
Boehringer Ingelheim Pharmaceuticals, Inc.





_______________________________________________________
Get 100% FREE Internet Access powered by Excite
Visit http://freelane.excite.com/freeisp



From daemon Thu Apr 27 11:02:25 2000



From: Roger Moretz :      rcmoretz-at-excite.com
Date: Wed, 26 Apr 2000 12:32:30 -0700 (PDT)
Subject: LM Ergonomic Stand

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Looking for the adjustable lucite bases made several years ago for adjusting
a LM for improved ergonomic access/use. I had the
information/supplier/manufacturer info around here someplace, but other than
the somewhat random neuronal firing, I appear to have
lost/misplace/misfiled/disposed of everything. I have a pathologist who
needs to incline his Nikon Microphot FX-A about 5 degrees. We have begun
the process of having a fixed wooden base constructed, but I much prefer the
lucite devices. Any help/ideas? TIA.


Roger C Moretz, Ph.D.





_______________________________________________________
Get 100% FREE Internet Access powered by Excite
Visit http://freelane.excite.com/freeisp



From daemon Thu Apr 27 11:02:25 2000



From: Roger Moretz :      rcmoretz-at-excite.com
Date: Wed, 26 Apr 2000 12:32:40 -0700 (PDT)
Subject: LM Ergonomic Stand

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Looking for the adjustable lucite bases made several years ago for adjusting
a LM for improved ergonomic access/use. I had the
information/supplier/manufacturer info around here someplace, but other than
the somewhat random neuronal firing, I appear to have
lost/misplace/misfiled/disposed of everything. I have a pathologist who
needs to incline his Nikon Microphot FX-A about 5 degrees. We have begun
the process of having a fixed wooden base constructed, but I much prefer the
lucite devices. Any help/ideas? TIA.


Roger C Moretz, Ph.D.





_______________________________________________________
Get 100% FREE Internet Access powered by Excite
Visit http://freelane.excite.com/freeisp



From daemon Thu Apr 27 11:02:43 2000



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Thu, 27 Apr 2000 09:24:58 +0100 (GMT Daylight Time)
Subject: Re: SEM charging effects

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


To reduce charging try reducing kV and spot size.

Dave


On Wed, 26 Apr 2000 15:13:14 +0800 Lam xu Fu Christopher
{eng81067-at-nus.edu.sg} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear Sir,
}
} I am currently using a JEOL 5800LV SEM.
} My specimens are cells from cultures which we have grown in our labs.
} The low vacuum condition is 7Pa.
} The problem occurs when I increase the magnification, about 400 times and
} beyond. The picture I get will be enlarged and clear except for certain
} regions which displays some glaring effects. Playing around with the
} contrasts and brightness didn't help.
} I was told this was a charging problem, and increasing the vacuum might
} help, so I tried. It did help a little but the picture quality was
} compromised.
}
} Are there other solutions to this problem of mine, such that the picture
} quality might remain the same.
}
} Chris Lam
} BIOMAT
} Mechanical & Production Department
} National University of Singapore
}
}
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"



From daemon Thu Apr 27 11:02:46 2000



From: Roger Moretz :      rcmoretz-at-excite.com
Date: Thu, 27 Apr 2000 04:30:45 -0700 (PDT)
Subject: Re: LM Ergonomic Stand

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Apologies for the multiple messages. The stupid excitemail server kept
telling me the message could not be sent and then deleted the message from
my inbox. So, I wrote another one, sent that, etc. Next time, I'll wait
until I see if the listserver sends the one through before resending. :-(
On Wed, 26 Apr 2000 12:32:40 -0700 (PDT),
rmoretz-at-rdg.boehringer-ingelheim.com wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} Looking for the adjustable lucite bases made several years ago for
adjusting
} a LM for improved ergonomic access/use. I had the
} information/supplier/manufacturer info around here someplace, but other
than
} the somewhat random neuronal firing, I appear to have
} lost/misplace/misfiled/disposed of everything. I have a pathologist who
} needs to incline his Nikon Microphot FX-A about 5 degrees. We have begun
} the process of having a fixed wooden base constructed, but I much prefer
the
} lucite devices. Any help/ideas? TIA.
}
}
} Roger C Moretz, Ph.D.
}
}
}
}
}
} _______________________________________________________
} Get 100% FREE Internet Access powered by Excite
} Visit http://freelane.excite.com/freeisp
}
}


Roger C Moretz, Ph.D.





_______________________________________________________
Get 100% FREE Internet Access powered by Excite
Visit http://freelane.excite.com/freeisp



From daemon Thu Apr 27 11:02:48 2000



From: Harmon.Stephen-at-epamail.epa.gov
Date: Thu, 27 Apr 2000 10:36:37 -0400
Subject: Re: SEM charging effects

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


|--------+-----------------------}
| | eng81067-at-nus.|
| | edu.sg |
| | |
| | 04/26/2000 |
| | 03:13 AM |
| | |
|--------+-----------------------}
} ---------------------------------------------------------|
| |
| To: Microscopy-at-sparc5.microscopy.com |
| cc: |
| Subject: SEM charging effects |
} ---------------------------------------------------------|











------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


} Dear Sir,

} I am currently using a JEOL 5800LV SEM.
} My specimens are cells from cultures which we have grown in our labs.
} The low vacuum condition is 7Pa.
} The problem occurs when I increase the magnification, about 400 times and
} beyond. The picture I get will be enlarged and clear except for certain
} regions which displays some glaring effects. Playing around with the
} contrasts and brightness didn't help.
} I was told this was a charging problem, and increasing the vacuum might
} help, so I tried. It did help a little but the picture quality was
} compromised.

} Are there other solutions to this problem of mine, such that the picture
} quality might remain the same.

} Chris Lam
} BIOMAT
} Mechanical & Production Department
} National University of Singapore

Chris,

I assume that your samples are not coated. 7 Pa may be too much of a vacuum
for uncoated samples; enough gas molecules are needed to help neutralize the
charge but too many tend to attenuate the signal. I generally operate the 5800
LV at 20-27 Pa without abundat charging artefacts. I would suggess 5 kV as
maximum gun potential and a probe current of 10 or less.

Cheers,


Stephen M. Harmon
Electron Microscopist
United States Environmental Protection Agency
M.S. 681
26 W. Martin Luther King Blvd.
Cincinnati, OH 45268
513.569.7184
Harmon.Stephen-at-epa.gov






From daemon Thu Apr 27 18:56:24 2000



From: Donald O'Leary :      donoleary-at-worldnet.att.net
Date: Thu, 27 Apr 2000 15:00:04 -0400
Subject: N.Y.M.S. Chemical Microscopy Workshop LM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


New York Microscopical Society

Bernard Friedman Memorial Workshop

Chemical Microscopy
May 20 & 21, 2000

A beginning course on chemical microscopy covering the basic techniques of
microchemical analysis using a microscope

The workshop will consist a weekend of lectures and hands on labs to cover
theoretical and practical aspects of chemical microscopy. We expect to
follow up with a second weekend course at a more advanced level for which
this will be a prerequisite. The course instructors will be the well known
and respected "Skip" Palenik of Microtrace, Inc. in Elgin, IL and N.Y.M.S.
Instructor Don O'Leary.

WHEN: May 20 & 21, 2000 from 10 A.M. to 4 P.M.

WHERE: 1244 McBride Avenue, West Paterson, NJ. Phone (973) 812-8377
(Free parking, accessible by public transportation, Information on car pools
and transportation will be provided.)

COST: $475 for N.Y.M.S. members, $495 for non-members (includes membership)
Lunch and course materials are included. Checks made out to N.Y.M.S.

WHO: Those with some basic knowledge of chemistry and chemical analysis and
use of the microscope.

HOW: Register using the form below. Limited to the first 12 registrants.
Return form to Don O'Leary, 6 Chittenden Road, Fair Lawn, NJ 07410.

FURTHER INFORMATION: Call D. O'Leary (201) 797 -8849

PLEASE POST
----------------------------------------------------------------------------
-------------------------------------
Registration Form
Chemical Microscopy

N.Y.M.S. Member_________________ ($475) Non-Member__________($495)

Name___________________________________________________________________
Address__________________________________________________________________
Phone (W)________________(H)______________________E-Mail___________________



From daemon Thu Apr 27 18:56:29 2000



From: Dusevich, Vladimir :      DusevichV-at-umkc.edu
Date: Thu, 27 Apr 2000 15:59:53 -0500
Subject: RE: SEM charging effects

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Are you sure it is a charging and not a burning?
But anyway you can try some these steps:
1. If you are using slow scan, increase frequency of scanning
and average frames.
2. Just decrease beam intencity and kV (may be you have too
nice beam) until an image become too noisy.
3. Decrease beam intencity. If you are using BSE you can try to
increase kV too. For SE (and I am not sure what type of it you
can have) try increase pressure and kV, or decrease pressure and kV.

Good luck


Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
3127 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy

} -----Original Message-----
} From: Lam xu Fu Christopher [mailto:eng81067-at-nus.edu.sg]
} Sent: Wednesday, April 26, 2000 2:13 AM
} To: 'Microscopy-at-MSA.Microscopy.Com'
} Subject: SEM charging effects
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
}
} --------------------------------------------------------------
} ---------.
}
}
} Dear Sir,
}
} I am currently using a JEOL 5800LV SEM.
} My specimens are cells from cultures which we have grown in our labs.
} The low vacuum condition is 7Pa.
} The problem occurs when I increase the magnification, about
} 400 times and
} beyond. The picture I get will be enlarged and clear except
} for certain
} regions which displays some glaring effects. Playing around with the
} contrasts and brightness didn't help.
} I was told this was a charging problem, and increasing the
} vacuum might
} help, so I tried. It did help a little but the picture quality was
} compromised.
}
} Are there other solutions to this problem of mine, such that
} the picture
} quality might remain the same.
}
} Chris Lam
} BIOMAT
} Mechanical & Production Department
} National University of Singapore
}
}
}



From daemon Thu Apr 27 18:56:29 2000



From: Pearl Martin :      image-at-optonline.net
Date: Thu, 27 Apr 2000 16:59:37 -0400
Subject: Job: Senior Metallographic Technician

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am a technical recruiter. Currently, I am working on a search for one
of my Northeast clients for a
Senior Metallographic Technician:


Will perform metallographic evaluation of coatings including work with
SEM (Scanning Electron
Microscopy) and X-ray. Will be responsible for the metallurgy
laboratory, including organization of
work, and operation and calibration of equipment. Will write technical
and engineering reports. Will
prepare projects for the metallurgy laboratory. Will maintain
communication with customers.

AAS/BS plus fours years experience in a metallography laboratory.

Resumes should be sent by email, mail or fax. For best results, please
send emails as attached
Word.docs - I have Word 97.

Pearl Martin
Image Associates Inc.
5254 Merrick Road
Massapequa, NY 11758
Phone (516)798-3993
Fax (516)797-8703
Email: pearl-at-jobspot.com





From daemon Thu Apr 27 19:27:13 2000



From: Michelle.Taurino-at-aventis.com
Date: Thu, 27 Apr 2000 19:04:01 -0500
Subject: Leica- EM stainer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am in the market for the Leica EM stainer. I was just looking for some
feedback (positive or negative) about this instrument...Any recommendations?

Michelle Taurino
Aventis Pharmaceuticals
Bioimaging and Molecular Histology
Tel-908-231-3357
Fax-908-231-3962
e-mail: Michelle.Taurino-at-aventis.com




From daemon Thu Apr 27 19:27:17 2000



From: Kristen Lennon :      kalen-at-citrus.ucr.edu
Date: Thu, 27 Apr 2000 15:34:17 -0700
Subject: diamond knife problems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All,
I am turning to all of you as a last ditch effort to see if my diamond
knife is crazy or I am. I have been using this same knife for years
(approximately 4 years) with the occasional difficulty. However, in the
past few weeks I have been having a very hard time getting sections. The
problem is this: water in the boat either wets the block or pulls away from
the knife edge. There seems to be no in-between. Every once in a while, the
stars seem to align, and the level achieves perfection for a few sections.
I've tried several different cleaning methods, thinking that maybe the
knife was dirty since I've been sectioning a lot of LR White. The only
thing I've noticed (since I've been looking so very closely at the diamond)
is that the seal (which was damaged when the knife arrived from the
manufacturer) between the knife and the boat has detached. I would think
that this would cause a leakage problem, which I haven't seen, not the
problem I'm experiencing.
Does anyone have any advice?
Thanks for your help,
Kristen
Kristen A. Lennon
Cell, Molecular & Developmental Biology Group
Department of Botany & Plant Sciences
University of California
Riverside, CA 92521
kalen-at-citrus.ucr.edu



From daemon Thu Apr 27 19:27:25 2000



From: Julian Smith III :      smithj-at-Winthrop.edu
Date: Thu, 27 Apr 2000 18:36:15 -0400
Subject: Cleaning closets....

Contents Retrieved from Microscopy Listserver Archives
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Hi, all:
We have the following that are surpus to our needs:
-Various bits of a Kevex 7000 mainframe (cards, keyboard)

-Bits of a Kevex Analyst 8000 mainframe (cards, keyboard, drives)

-Software and manuals for both of the above systems

-Fisher Code-on histomatic slide stainer (given to us as "working", but not
tested)

-EG&G Ortec system 5000. Bad power supply.

Any of the above free to non-profits for the cost of shipping.

If you're a commercial entity and would like any of the above, let's talk
trade for some of your junque... (preferably, *smaller* junque)

contact me at telephone# below or via e-mail at smithj-at-winthrop.edu

cheers,
Julian

Julian P.S. Smith III
Dept. of Biology
Winthrop University
Rock Hill, SC 29733
803-323-2111 x6427 (vox)
803-323-3448 (fax)




From daemon Thu Apr 27 19:27:30 2000



From: M. Baxter :      mykkb-at-juno.com
Date: Thu, 27 Apr 2000 18:49:55 -0400
Subject: Re: SEM Charging

Contents Retrieved from Microscopy Listserver Archives
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Tilting the sample toward the detector (up to about 45 degrees) helps
eliminate some types of charging. It is easy enough and worth a try
before more involved techniques are tried.


Mike Baxter
Lehman College

________________________________________________________________
YOU'RE PAYING TOO MUCH FOR THE INTERNET!
Juno now offers FREE Internet Access!
Try it today - there's no risk! For your FREE software, visit:
http://dl.www.juno.com/get/tagj.



From daemon Thu Apr 27 19:27:33 2000



From: Purdy, Sam :      SPurdy-at-nationalsteel.com
Date: Thu, 27 Apr 2000 19:18:43 -0500
Subject: RE: permanganate etching

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Petra:
Are you using "permanganate etch" on a metallographic specimen? If so,
Vander Voort's book "Meatallogrphy: Principals and Practice" covers the use
of permanganate etches. See also Petzow's book on etching, in German or
English.

Sam Purdy
National Steel Technical Center
Trenton, MI, USA
} ----------
} From: Petra Wahlbring
} Sent: 26, April 2000, 4:55 AM
} To: microscopy-at-sparc5.microscopy.com
} Subject: TEM: permanganate etching
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear colleagues,
}
} I have a few PE specimens on my desk that I want to prepare with the
} "permanganate etching method". Does anybody of you have some experience on
} this techique? Would you share some details or tips and tricks with me on
} how to obtain the best results? What is the best method to produce a
} replica from the etched specimen?
}
} Petra
} --------------------------------------------------------------
} Dr. Petra Wahlbring
} Centre de Recherche Public - Gabriel Lippmann
} Laboratoire d'Analyse des Materiaux (LAM)
} 162a, av. de la Faiencerie L-1511 Luxembourg
} tel. +352-466644-402 fax +352-466644-400
} e-mail: petra.wahlbring-at-crpgl.lu
} Visit our WWW site! http://www.crpgl.lu/~wahlbrin
}




From daemon Fri Apr 28 08:06:03 2000



From: RCHIOVETTI-at-aol.com
Date: Thu, 27 Apr 2000 20:52:43 EDT
Subject: Re: diamond knife problems

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In a message dated 04/27/2000 5:27:39 PM US Mountain Standard Time,
kalen-at-citrus.ucr.edu writes:

{ { water in the boat either wets the block or pulls away from
the knife edge. There seems to be no in-between. Every once in a while, the
stars seem to align, and the level achieves perfection for a few sections.
I've tried several different cleaning methods, thinking that maybe the
knife was dirty since I've been sectioning a lot of LR White. The only
thing I've noticed (since I've been looking so very closely at the diamond)
is that the seal (which was damaged when the knife arrived from the
manufacturer) between the knife and the boat has detached. I would think
that this would cause a leakage problem, which I haven't seen, not the
problem I'm experiencing. } }

Kristen,

I had a knife that behaved similarly once upon a time. Let's assume the boat
isn't leaking, since you aren't having problems with water collecting
underneath the knife in the knife holder, correct? It would be difficult to
imagine the knife has come loose in the boat, but that may be a very slight
possibility. If it were loose in its mount it might not section with
consistency. Either way, it would be a good idea to have the knife re-sealed
properly.

The knife I had was almost impossible to get the proper amount of wetting on
the diamond, but two things helped:

1. Try deliberately overfilling the boat to give a positive meniscus right
at the knife edge. Leave the knife in this state for 15-20 minutes. Relax,
go have a cup of coffee, whatever. Then try lowering the fluid to the proper
level. This worked well for me.

2. For really difficult knives that refuse to wet, just a very little bit of
saliva on the end of a dalmatian hair works great. A light brush against the
tongue, then drag the hair in the boat fluid behind the knife edge. I know
it sounds gross, but it works! Others have used Alconox crystals,
detergents, etc. to break the surface tension, but good old saliva works
every time.

Hope this helps.

Cheers,

Bob Chiovetti
GTI Microsystems



From daemon Fri Apr 28 08:06:21 2000



From: Mohamed Belhaj :      mohamed.belhaj-at-univ-reims.fr
Date: Fri, 28 Apr 2000 10:28:53 +0200
Subject: Re: SEM charging effects

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IIn the following references, methods to adjust the best operating conditions
(scan rate, Energy, current ) for SEM of uncoated insulators samples are
proposed and discussed.

D. C. Joy, Scanning 11, 1 (1989).
D. C Joy and C. S. Joy , Micron. 27, 247 (1996).

Good luck



*****************************************************************
Mohamed Belhaj
UFR SCIENCES
Laboratoire d'Analyse des Solides Surfaces et Interfaces
DTI/LASSI UMR CNRS
BP 1039
Reims 51687 Cedex 2
Tel : (00 33) 03 26 91 33 27: (00 33) 03 26 91 33 14
Fax : (00 33) 03 26 91 33 12
******************************************************************



From daemon Fri Apr 28 08:06:26 2000



From: DDKJoe-at-aol.com
Date: Fri, 28 Apr 2000 07:24:07 EDT
Subject: Re: diamond knife problems

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Karen,

Bob Chiovetti's ideas are right on the mark. It sounds crazy but saliva
works wonders. The only thing I would change would be the tool to use to
apply the saliva. It would probably be safer if you used the styrofoam stick
provided by many diamond knife or EM supply folks. Clean the knife with the
stick according to the instructions using a "bit of spit". Follow this up
with DI water since saliva is surprisingly dirty.

Should restore any wetting properties your old knife has left.

Have fun,
Joe Tabeling
Delaware Diamond Knives, Inc.
800-222-5143



From daemon Fri Apr 28 08:30:24 2000



From: Emrah Yucelen :      hvtem-at-yahoo.com
Date: Fri, 28 Apr 2000 08:07:46 -0500
Subject: information about TEM and radiation

Contents Retrieved from Microscopy Listserver Archives
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dear colleagues,


I wonder about TEM and radiation emitting.
is there some dangerous situation for old TEMs?
does anybody have an information about this.

Emrah YUCELEN


IstanbulTechnical University
Metallurgical
and Materials Engineering

ElectronMicroscopy Laboratory


__________________________________________________
Do You Yahoo!?
Talk to your friends online with Yahoo! Messenger.
http://im.yahoo.com




From daemon Fri Apr 28 15:43:36 2000



From: Jan Watkins :      jwatkins-at-conv1.nrlssc.navy.mil
Date: Fri, 28 Apr 2000 08:53:18 -0500
Subject: Please post this question

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I am interested in getting information regarding processing images,
specifically clay fabric using Adobe PhotoShop and Image Tool, (plug-ins and
"The Image Processing Handbook"). Can anyone provide me with information on
a short course or 3 to 5 day workshop on this subject?



From daemon Fri Apr 28 15:43:38 2000



From: Caroline Schooley :      schooley-at-mcn.org
Date: Fri, 28 Apr 2000 08:52:11 -0700
Subject: Re: diamond knife problems

Contents Retrieved from Microscopy Listserver Archives
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} I am turning to all of you as a last ditch effort to see if my diamond
} knife is crazy or I am. I have been using this same knife for years
} (approximately 4 years) with the occasional difficulty. However, in the
} past few weeks I have been having a very hard time getting sections. The
} problem is this: water in the boat either wets the block or pulls away from
} the knife edge. There seems to be no in-between. Every once in a while, the
} stars seem to align, and the level achieves perfection for a few sections.
} I've tried several different cleaning methods, thinking that maybe the
} knife was dirty since I've been sectioning a lot of LR White. The only
} thing I've noticed (since I've been looking so very closely at the diamond)
} is that the seal (which was damaged when the knife arrived from the
} manufacturer) between the knife and the boat has detached. I would think
} that this would cause a leakage problem, which I haven't seen, not the
} problem I'm experiencing.

} Kristen -

Sounds like you're describing a slow leak, which will lower the water level
& wet the back of the knife. Try the "old-fashoned" knife sealing method:
Melt a bit of dental wax on your slide-warmer, and warm the knife gently
while the wax is melting. Use a toothpick to run a bit of wax into the
cracked seal.


Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/PCI/pci.html




From daemon Fri Apr 28 15:43:39 2000



From: Jo Dee :      jofish-at-burnham-inst.org
Date: Fri, 28 Apr 2000 09:39:25 -0700
Subject: Re: diamond knife problems

Contents Retrieved from Microscopy Listserver Archives
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This sounds crazy, but my new knife came from Micro Star Diamond knives, and they
recommend only using dish detergent to wash their diamond knives. When I tried
this, the first thing that I noticed is how easy it is to keep the knife wet. I
first tried it with a problem knife, and it worked! Also, I don't have the block
face wetting problem that I had had before.
Try it, you'll be surprised.
Jo Dee
PS. I am not affiliated with Micro Star!

"RCHIOVETTI-at-aol.com"-at-sparc5.microscopy.com wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} In a message dated 04/27/2000 5:27:39 PM US Mountain Standard Time,
} kalen-at-citrus.ucr.edu writes:
}
} { { water in the boat either wets the block or pulls away from
} the knife edge. There seems to be no in-between. Every once in a while, the
} stars seem to align, and the level achieves perfection for a few sections.
} I've tried several different cleaning methods, thinking that maybe the
} knife was dirty since I've been sectioning a lot of LR White. The only
} thing I've noticed (since I've been looking so very closely at the diamond)
} is that the seal (which was damaged when the knife arrived from the
} manufacturer) between the knife and the boat has detached. I would think
} that this would cause a leakage problem, which I haven't seen, not the
} problem I'm experiencing. } }
}
} Kristen,
}
} I had a knife that behaved similarly once upon a time. Let's assume the boat
} isn't leaking, since you aren't having problems with water collecting
} underneath the knife in the knife holder, correct? It would be difficult to
} imagine the knife has come loose in the boat, but that may be a very slight
} possibility. If it were loose in its mount it might not section with
} consistency. Either way, it would be a good idea to have the knife re-sealed
} properly.
}
} The knife I had was almost impossible to get the proper amount of wetting on
} the diamond, but two things helped:
}
} 1. Try deliberately overfilling the boat to give a positive meniscus right
} at the knife edge. Leave the knife in this state for 15-20 minutes. Relax,
} go have a cup of coffee, whatever. Then try lowering the fluid to the proper
} level. This worked well for me.
}
} 2. For really difficult knives that refuse to wet, just a very little bit of
} saliva on the end of a dalmatian hair works great. A light brush against the
} tongue, then drag the hair in the boat fluid behind the knife edge. I know
} it sounds gross, but it works! Others have used Alconox crystals,
} detergents, etc. to break the surface tension, but good old saliva works
} every time.
}
} Hope this helps.
}
} Cheers,
}
} Bob Chiovetti
} GTI Microsystems



From daemon Fri Apr 28 15:43:39 2000



From: beth-at-dogwood.botany.uga.edu (Beth Richardson)
Date: Fri, 28 Apr 2000 11:03:32 -0500
Subject: thanks for digital camera info

Contents Retrieved from Microscopy Listserver Archives
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I just want to say thanks for all the replies about the digital camera for
copy stand use. The info is much appreciated.
best regards,
Beth

**************************************
Beth Richardson
EM Lab Coordinator
Botany Department
University of Georgia
Athens, GA 30602

Phone - (706) 542-1790
FAX - (706) 542-1805
Email - beth-at-dogwood.botany.uga.edu
**************************************




From daemon Fri Apr 28 15:43:39 2000



From: phil.swab-at-depsci.com (Phil Swab)
Date: Fri, 28 Apr 2000 08:34:17 -0700
Subject: diamond knife problems

Contents Retrieved from Microscopy Listserver Archives
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Kristen,
It may be that after 4 years your knife edge needs a good cleaning.
I've found that when I start to have trouble wetting the knife edge I can
treat/clean the knife with a dilute solution of household ammonia.
However, remember the knife housing is made of anodized aluminum,
prolonged exposure to ammonia will degrade the aluminum and the diamond
bonding. After soaking your knife and cleaning it in your normal way,
leave it wet, and clean it again in a dilute solution of ammonia, carefully
cleaning the knife edge with a pithwood stick. Then rinse thoroughly in
flowing water for several minutes.

Phil Swab
Engineering Development
Deposition Sciences Inc.
Santa Rosa, CA
707-566-3718
phil.swab-at-depsci.com

-----Original Message-----
} From: Kristen Lennon [SMTP:kalen-at-citrus.ucr.edu]
Sent: Thursday, April 27, 2000 3:34 PM
To: Microscopy-at-sparc5.microscopy.com


Hi All,
I am turning to all of you as a last ditch effort to see if my diamond
knife is crazy or I am. I have been using this same knife for years
(approximately 4 years) with the occasional difficulty. However, in the
past few weeks I have been having a very hard time getting sections. The
problem is this: water in the boat either wets the block or pulls away from
the knife edge. There seems to be no in-between. Every once in a while, the
stars seem to align, and the level achieves perfection for a few sections.
I've tried several different cleaning methods, thinking that maybe the
knife was dirty since I've been sectioning a lot of LR White. The only
thing I've noticed (since I've been looking so very closely at the diamond)
is that the seal (which was damaged when the knife arrived from the
manufacturer) between the knife and the boat has detached. I would think
that this would cause a leakage problem, which I haven't seen, not the
problem I'm experiencing.
Does anyone have any advice?
Thanks for your help,
Kristen
Kristen A. Lennon
Cell, Molecular & Developmental Biology Group
Department of Botany & Plant Sciences
University of California
Riverside, CA 92521
kalen-at-citrus.ucr.edu




From daemon Fri Apr 28 15:43:40 2000



From: Kristen Lennon :      kalen-at-citrus.ucr.edu
Date: Fri, 28 Apr 2000 12:11:41 -0700
Subject: diamond knife problems

Contents Retrieved from Microscopy Listserver Archives
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I want to thank everyone who responded to my plea for help with my diamond
knife problems. I am trying a variety of your suggestions. The strange, but
overwhelming theme involved saliva as a wetting and even a cleaning agent.
As I sit here (after trying a couple of suggestions), a beautiful ribbon of
sections is forming in the boat. I hope that it holds up.
Thanks,
Kristen
Kristen A. Lennon
Cell, Molecular & Developmental Biology Group
Department of Botany & Plant Sciences
University of California
Riverside, CA 92521
kalen-at-citrus.ucr.edu



From daemon Fri Apr 28 15:43:41 2000



From: William Tivol :      tivol-at-wadsworth.org
Date: Fri, 28 Apr 2000 10:06:15 -0400
Subject: Re: information about TEM and radiation

Contents Retrieved from Microscopy Listserver Archives
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Emrah Yucelen wrote:

} I wonder about TEM and radiation emitting.
} is there some dangerous situation for old TEMs?
} does anybody have an information about this.
}

Dear Emrah,
There could be x-rays generated by some old
TEMs. We had a JEOL JEM-200 which emitted a
stream of x-rays near the specimen airlock. Not only
did this make it a bad idea to stand up at the micro-
scope when the beam was on, it was also a danger for
other people in the adjacent lab. The best way to see
if there is a problem is to use a monitor (either a
Geiger counter or an ionization chamber) and survey
all around the instrument with the beam on, a heav-
ily-stained specimen in the scope, and the settings
at extreme positions. You want to survey at the
worst possible conditions that a user could set up.
Remember that x-rays can penetrtate the floor, so
the area beneath the scope should be surveyed also
(unless the scope is in the basement with no one
able to work beneath it). It should take only a few
hours to complete such a survey. Shielding for ~100
kV scopes is not too difficult in case there are radia-
tion leaks. Resurvey after the shielding is in place.
Good luck.
Yours,
Bill Tivol



From daemon Fri Apr 28 15:43:41 2000



From: Goodhouse, Joseph :      jgoodhouse-at-molbio.Princeton.EDU
Date: Fri, 28 Apr 2000 15:47:39 -0400
Subject: Bad Probes & Misinformation

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K. Spencer wrote about Molecular Probes' Bodipy phalloidin not working or
being excited by 488 nms, 568 and 647 laser lines. I would like to know
which Bodipy you are referring to. I find it improper and misleading to
talk about a reagent unless you are going to include your specifics. I have
used several of these reagents in the past with excellent results. If you
checked the probes catalogue you will note that there are 3 Bodipy
phalloidins listed ( Bodipy 529/547, 558/569 and 584/592 ) along with
several Bodipy phallicidins that also bind to filamentous actin. These are
Bodipy 502 /512 and Bodipy-TR-X 589/617. The numbers refer to the
excitation peak and the emission peak respectively. None of these probes
should be excited with any efficiency by a 647 laser. You might see some if
you are over stained with the 584/592, a common mistake with my users.
This probe would be excited by a 568 laser but not by a 488 nm line.
Secondly, before buying a probe, one is advised to find out what is
excitation and emission spectra are ( not just peak numbers) so you know
what possible cross talks one could possibly experience.

For these reagents I recommend the Bodipy 505/512 or Oregon Green for 488
excitation. AlexaFluor-568 phalloidin or rhodamine phalloidin for
excitation with 543 or 568 laser. The Alexafluors would be my top choice
because of superior spectral properties. I know of none that work with a
633 or 647 laser. Cy 5 is my preferred choice for the far red channel as a
conjugated antibody and TOTO-3 as a DNA marker.

Recently someone complained of TOTO-3 bleaching as they imaged. The dye
doesn't bleach, but rather gets displaced from the DNA when laser excites
it. It only fluoresces when bound to DNA or RNA. The trick here is to use
the lowest laser or excitation you can, and to include the dye at about 1-2
micromolar in your mounting medium so it can re-intercalate into the DNA.


Joe Goodhouse
Confocal / EM Core Laboratory
Department of Molecular Biology
Princeton University

Visit us at http://www.molbio.princeton.edu/facility/confocal/index.html
{http://www.molbio.princeton.edu/facility/confocal/index.html}

jgoodhouse-at-molbio.princeton.edu {mailto:Jgoodhouse-at-molbio.princeton.edu}
609-258-5432



From daemon Fri Apr 28 18:07:53 2000



From: Douglas Keene :      DRK-at-shcc.org
Date: Fri, 28 Apr 2000 13:51:09 -0700 (Pacific Daylight Time)
Subject: Re: diamond knife problems

Contents Retrieved from Microscopy Listserver Archives
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Hello Kristen,

I concur with Bob Chiovetti's suggestions as possible
solutions to aid in wetting of the diamond knife edge and
eliminate block wetting. In particular, it seems that
adding a bit of saliva to your eyelash tool, which is then
wiped across the flat portion of the diamond, is very
effective. In extreme cases we have subjected the knife to
ionized air via glow discharge but this can cause water to
be drawn to the back side of the diamond. Naturally, you
should check the diamond for cleanliness. It may help to
immerse the knife in soapy water after careful cleaning
with ethanol dipped Styrofoam. After rinsing the soap away
with purified water, we then rinse the boat in ethanol
which also seems to help wetting of the edge.

Good luck,

Doug
----------------------
Douglas R. Keene
Associate Investigator
Shriners Hospital Research Facilities
3101 S.W. Sam Jackson Park Road
Portland, Oregon 97201
phone: 503-221-3434
FAX: 503-412-6894 (9-5 PST)
e-mail: DRK-at-shcc.org




From daemon Fri Apr 28 18:07:54 2000



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Fri, 28 Apr 2000 11:24:02 -1000 (HST)
Subject: Re: Holder Filter

Contents Retrieved from Microscopy Listserver Archives
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}
} I am searching some device for holding (polycarbonate)filters during
} dehydration and CPD. I am trying to view mycoplasms with SEM. Has anyone
} experience with this ?
}
We have a device from Tousimis that is designed to hold round glass
coverslips, and I have used it to hold polycarbonate filters. It is a
milled-out cylinder open along one side. Wavy washers are used to
separate the stacked coverslips. There are two sizes, 13 mm and 22 mm,
part numbers 8766 and 8767. You can contact Tousimis at 1-800-638-9558,
fax 1-301-881-5374, http://www.tousimis.com

This device works for me! My only affiliation with Tousimis is as a
satisfied customer.

Aloha,
Tina

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************



From daemon Fri Apr 28 18:07:55 2000



From: anderron-at-us.ibm.com
Date: Fri, 28 Apr 2000 10:58:41 -0400
Subject: Lehigh TEM Specimen Preparation Course

Contents Retrieved from Microscopy Listserver Archives
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The TEM specimen preparation course advertised in the Lehigh "2000
Microscopy School" booklet, p. 20., has undergone revision. This is a
completely new course that was not finalized when it was time to print the
booklet. The material on p.20 was a place holder.

Scott Walck joined with me in organizing the course and we have secured
commitments from a large number of specimen preparation tool vendors to
provide staff and equipment for the course, listed below.

Title:

TEM Specimen Preparation 2000
With Emphasis on Recently Developed Tools and Methods

Course Description:

This course is designed to provide classroom instruction and laboratory
demonstration of the newest methods for preparing SEM and TEM specimens.
While an individual with no experience preparing specimens will benefit
greatly from the course, the intended audience will consist of students
with some degree of proficiency utilizing classical methods of specimen
preparation and who wish to update their capabilities. Emphasis will be
placed on the rapid preparation of specimens from very small pre-selected
locations. The preparation of SEM samples will be treated as the first
step in making TEM specimens. "Hands on" experience by the students will
be available. Table-top exhibits and demonstrations of specimen
preparation ancillary equipment, such as: saws, dimplers, disc cutting
tools, etc., will be available during the lab periods. TEM examination of
prepared specimens will be performed.

Instructors:

Ron Anderson, IBM Analytical Services and Scott Walck, PPG Industries, Inc.

Outline:

Thursday, June 22

10:00 am Registration (Whitaker Lobby)
1:00 pm Introduction Anderson/Walck
1:15 pm Specimen Preparation Flowchart,
Initial Considerations, Recent Advances,
Choice of Technique Anderson
2:15 pm Initial Thinning, Tools, Disk Cutting,
Dimpling Walck
3:30 pm Mechanical Polishing Anderson
4:30 pm Automated Initial Preparation Methods Vendors
Sawing: Sagitta
Cleaving: SELA
7:45 pm Ion Milling Anderson
Commercial Tool Overview Walck

Friday, June 23

8:30 am Lab: Tripod Polishing
Lab: Sagitta Tool
Lab: SELA Tool
11:00 am Focused Ion Beam (FIB) Methods Anderson/Walck
2:00 pm Lab: FIB Methods
Lab: Ion Mill Tools

Saturday, June 24


8:30 am Mechanical Polishing Difficult Materials Anderson
9:00 am Ultramicrotomy Walck/Anderson
10:00 am Small Angle Cleavage Technique Video Igor
10:30 am Plasma Cleaning Specimens Walck
11:00 am Reactive Sample/Storage/Transporting Walck
11:15 am Lab: Student Use of Available Tools
Lab: TEM Examination of Prepared
Specimens. Discussion of Successes
and Problems

Vendors providing instrumentation and staff:
v Allied High Tech
v Bal-Tec
v FEI
v EA Fischione
v Gatan
v Sagitta
v SELA
v South Bay Technology

The registration deadline is June 1st. Registration forms are in the
"Lehigh Microscopy School" booklet, or they may be obtained, along with
accommodation information etc., from :

Ms. Sharon Coe
Dept. of Materials Science and Engineering
Lehigh University
5 East Packer Avenue
Bethlehem, PA 18015-3195

(610)758-5133
(610)758-4244 FAX
SHARON.COE-at-LEHIGH.EDU

www.lehigh.edu/~inmatsci/Microscourses.html






Ron Anderson, IBM, Hopewell Jct., New York, USA. anderron-at-us.ibm.com

IBM Analytical Services; http://www.chips.ibm.com/services/asg





From daemon Fri Apr 28 18:07:56 2000



From: HILDEGARD CROWLEY :      hcrowley-at-du.edu
Date: Fri, 28 Apr 2000 12:23:40 -0600 (MDT)
Subject: Re: diamond knife problems

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On Fri, 28 Apr 2000 DDKJoe-at-aol.com-at-sparc5.microscopy.com wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Karen,
}
} Bob Chiovetti's ideas are right on the mark. It sounds crazy but saliva
} works wonders. The only thing I would change would be the tool to use to
} apply the saliva. It would probably be safer if you used the styrofoam stick
} provided by many diamond knife or EM supply folks. Clean the knife with the
} stick according to the instructions using a "bit of spit". Follow this up
} with DI water since saliva is surprisingly dirty.
}
} Should restore any wetting properties your old knife has left.
}
} Have fun,
} Joe Tabeling
} Delaware Diamond Knives, Inc.
} 800-222-5143
}
}
Hi,

Saliva is dirty and full of debris and bacteria. Use a wetting agent
instead as above and fill the boat with it or draw it across the edge
whichever works best.

Hildy Crowley



From daemon Fri Apr 28 18:07:56 2000



From: HILDEGARD CROWLEY :      hcrowley-at-du.edu
Date: Fri, 28 Apr 2000 12:20:42 -0600 (MDT)
Subject: Re: diamond knife problems

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On Thu, 27 Apr 2000, Kristen Lennon wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
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} -----------------------------------------------------------------------.
}
}
} Hi All,
} I am turning to all of you as a last ditch effort to see if my diamond
} knife is crazy or I am. I have been using this same knife for years
} (approximately 4 years) with the occasional difficulty. However, in the
} past few weeks I have been having a very hard time getting sections. The
} problem is this: water in the boat either wets the block or pulls away from
} the knife edge. There seems to be no in-between. Every once in a while, the
} stars seem to align, and the level achieves perfection for a few sections.
} I've tried several different cleaning methods, thinking that maybe the
} knife was dirty since I've been sectioning a lot of LR White. The only
} thing I've noticed (since I've been looking so very closely at the diamond)
} is that the seal (which was damaged when the knife arrived from the
} manufacturer) between the knife and the boat has detached. I would think
} that this would cause a leakage problem, which I haven't seen, not the
} problem I'm experiencing.
} Does anyone have any advice?
} Thanks for your help,
} Kristen
} Kristen A. Lennon
} Cell, Molecular & Developmental Biology Group
} Department of Botany & Plant Sciences
} University of California
} Riverside, CA 92521
} kalen-at-citrus.ucr.edu
}
}
The detachment of the knife from its mount may be the cause. It has
happened in our laboratory. Try, however, to add one drop of Photo-flo to
about 50ml of filtered or distilled water. Try that in the boat.
Detachment tends to be a serious problem! Send it back and have it
repaired and resharpened. After 4 years it probably needs it.

Hildy Crowley



From daemon Fri Apr 28 18:07:56 2000



From: Steve Barlow :      sbarlow-at-sunstroke.sdsu.edu
Date: Fri, 28 Apr 2000 15:09:12 -0700
Subject: parts from old equipment

Contents Retrieved from Microscopy Listserver Archives
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Spring cleaning and new equipment requires I dispose of a Mikros VE10
vacuum evaporator and a LKB 4800 ultramicrotome. Anyone needing parts
from these old, non-functional units please contact me.

steve


Dr. Steven Barlow, Associate Director
EM Facility/Biology Department
San Diego State University
5500 Campanile Drive
San Diego CA 92182-4614
phone: (619)594-4523
fax: (619) 594-5676
email: sbarlow-at-sunstroke.sdsu.edu
website: http://www.sci.sdsu.edu/emfacility/

Chairman, Educational Outreach subcommittee
promoting access to microscopes
Microscopy Society of America http://www.msa.microscopy.com/




From daemon Sat Apr 29 08:15:09 2000



From: Damian :      dneuberger-at-mindspring.com
Date: Fri, 28 Apr 2000 22:46:33 -0500
Subject: Re: digital cameras for copy stand work?

Contents Retrieved from Microscopy Listserver Archives
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Beth,

You may want to consider a digital camera that is a SLR camera. I have a
Kodak DC 120 and it works reasonably well but when I want to take close up
photos or copy stand shots, I have to take several exposures to get the
subject critically centered. Of course, a SLR allows me to set up my photo
exactly the way I want it; also getting the correct lighting is
easier. For this kind of work I use a Kodak DCS 420, but at ~$12k it's a
"bit expensive".



} Hi all,
} This is off the microscopy subject...sorry.
} Any recommendations on digital cameras for copy stand work and general
} photography? Has anyone tried the Nikon Coolpix 990?
} We're coming up on the end of the fiscal year and there might be funds
} available for a digital camera so any advice is greatly appreciated.
} thanks,
} Beth Richardson

Damian Neuberger, Ph.D.
Baxter Healthcare, Inc.
WG3-2S
P.O. Box 490
Round Lake, IL 60073
e-mail: damian_neuberger-at-baxter.co,
Tel: 847-270-5888
Fax: 847-270-5897



From daemon Sat Apr 29 08:15:09 2000



From: Damian :      dneuberger-at-mindspring.com
Date: Fri, 28 Apr 2000 19:47:33 -0500
Subject: Re: Kodak MDS 120

Contents Retrieved from Microscopy Listserver Archives
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The Kodak MDS 120 does a very good job for the price. My only
recommendation is that you get the flash memory cards (I have a 64 MB card)
and a card reader or adapter for a PCMCIA slot. Transferring the images
via the built-in serial port is, of course, INCREDIBLY SLOW!!! The
included software works well and you can easily get the images into other
software such as Adobe Photoshop.




} Part of the package which we got is a 16 MB Flash Memory card for the camera,
} so you can either run the camera from your computer or store images on the
} memory card and then remove the card and take it to a computer to transfer
} the images to the computer. The package also had a PCMCIA card adapter in
} it. The memory card plugs into the end of the PCMCIA device, and you can
} then slip it into a PCMCIA slot on a laptop computer.
}
} But if you need to use a desktop computer and don't have a PCMCIA slot, you
} need to get something like a SanDisk card reader for the computer. They're
} available at CompUSA, Fry's Electronics, OfficeMax, etc. for about $70.
}
} The software with the camera is very nice. It lets you transfer images from
} the camera's memory directly or from from the memory card, preview images and
} download them directly from the camera, etc. as well as perform some basic
} image adjustments (flip, rotate, modify brightness, contrast, gamma, etc.)

Damian Neuberger, Ph.D.
Baxter Healthcare, Inc.
WG3-2S
P.O. Box 490
Round Lake, IL 60073
e-mail: damian_neuberger-at-baxter.co,
Tel: 847-270-5888
Fax: 847-270-5897



From daemon Sat Apr 29 08:15:10 2000



From: Damian :      dneuberger-at-mindspring.com
Date: Fri, 28 Apr 2000 22:45:07 -0500
Subject: RE: digital cameras for copy stand work?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Just returned from a course on VP SEM (which was excellent) and just now
can add to some of the digital camera discussion.

One thing not mentioned about using a SLR digital camera like the E1, N60
is vibration from the mirror and the shutter. If you are going to use a
camera of this type you must be able to lift the mirror independently, like
the old F1, whether it's film or CCD. I have a Kodak DCS420 that is based
on the Nikon N90s camera body and it works ok at low magnifications but
anything over about 200X just doesn't cut it.



At 08:33 AM 4/26/00 -0700, Dr. Gary Gaugler wrote:
} The Fuji Super CCD is 23.3 x 15.6mm; 3040 x 2016 pixels.
} The S1 Pro uses Nikon mount lenses and has an equivalent
} 35mm frame size at 1.5X the lens' focal length.
}
} What the equivalent FOV would be on a scope is still in
} question. If the S1 Pro works anywhere near how the
} Nikon E1 or E2 does, I fear the Fuji camera won't be applicable
} to microscopy at all. Without a lens, the camera must operate
} in aperture priority mode and center weighted exposure
} reading. Unless Fuji made some major changes to the basic
} Nikon (N-60) body and associated electronics, they may have
} just made a higher pixel count Nikon digicam.

Damian Neuberger, Ph.D.
Baxter Healthcare, Inc.
WG3-2S
P.O. Box 490
Round Lake, IL 60073
e-mail: damian_neuberger-at-baxter.co,
Tel: 847-270-5888
Fax: 847-270-5897



From daemon Sat Apr 29 09:45:31 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Sat, 29 Apr 2000 07:34:07 -0700
Subject: RE: digital cameras for copy stand work?

Contents Retrieved from Microscopy Listserver Archives
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Neither the N60 or N80 bodies have mirror lock up. However,
at equivalent ISO of 400-1600, it remains to be seen if lock
up is necessary. And also, how ISO affects the resulting
image. Based on how the E1 and E2 worked, I would not
count on a miracle.

The Kodak 420 is obsolete and discontinued. These can be
purchased used today for somewhere in the $2K-$4K range.
But, with its tiny CCD, I would not have one again. The newer
models like DCS460 have good features. But I have not
been impressed by Kodak's tech support user friendliness.
It would be a hard sell for me to buy any Kodak product these
days over other manufacturer's offerings.

The mechanical shutter is a definite problem, both for image
stability as well as camera reliability. The Leaf Lumina has a
shutter but it operates only one time for each shot. It is very
reliable. In contrast, the Polaroid DMC has a shutter that seems
to always be "shuddering" the system. I would expect that the
DMC would exhibit a poor reliability record. The advantage of
the DMC is that it provides near-real time focusing through the
camera's imager. The only other camera I have used that
exceeds this is the Sony DKC-5000 (cat's eye) digital camera.
But the DKC-5000 uses a very small CCD as well and suffers
from tiny images. But focusing is true real time via a separate
RGB color monitor. When a frame is shot, the main system
box transfers the image to the computer via SCSI.

gg

At 08:45 PM 4/28/00 , you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Sun Apr 30 09:37:43 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Sat, 29 Apr 2000 17:56:06 -0700
Subject: Freon 113 dehydration

Contents Retrieved from Microscopy Listserver Archives
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When using F-113, I preceed it with either or both
methanol and acetone. The curious problem is that
after either a lengthy immersion in acetone or methanol,
the specimen (insect) floats when put in F-113. OK.
So it is lighter than F-113. What is a good method
of encompassing the specimen with F-113 to further
dehydrate it prior to vacuum desiccation?

Any ideas?



~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Modern surfers use PC boards. You can too at
http://photoweb.net
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~



From daemon Sun Apr 30 09:37:46 2000



From: John Foust :      jfoust-at-threedee.com
Date: Sat, 29 Apr 2000 21:18:51 -0500
Subject: Introductory message

Contents Retrieved from Microscopy Listserver Archives
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I'm new to the list. I recently picked up a delightful
Leitz Ortholux I, like at http://arsmachina.com/leitz_ortholux.htm
and a non-working Zeiss OpMi-1 surgical scope, similar to
the upper left quadrant of http://www.mednetlocator.com/opmi-1.gif
with not much arm and no stand. I think it's missing a lense
from the underside.

I'm temporarily illuminating the Ortholux with the 5V DC from a
spare PC power supply. The lamp says 6 volts, I think. What's
the voltage range from the original rheostat-style power supply?

- John



From daemon Sun Apr 30 09:37:49 2000



From: COURYHOUSE-at-aol.com
Date: Sun, 30 Apr 2000 00:03:43 EDT
Subject: Re: Introductory message

Contents Retrieved from Microscopy Listserver Archives
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Hi John!
5 volts is fine, the color temperature is a bit warmer but your bulb will
last longer!
I usually only run the bulb at full or over when I am taking a photograph.
For normal observation I find the 5 volts fine. Congrats on the othrolux
great classic scope!
Ed Sharpe archivist for SMECC

{ { Subj: Introductory message
Date: 4/29/00 8:53:44 PM US Mountain Standard Time
From: jfoust-at-threedee.com (John Foust)
To: Microscopy-at-sparc5.microscopy.com

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America
To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
-----------------------------------------------------------------------.



I'm new to the list. I recently picked up a delightful
Leitz Ortholux I, like at http://arsmachina.com/leitz_ortholux.htm
and a non-working Zeiss OpMi-1 surgical scope, similar to
the upper left quadrant of http://www.mednetlocator.com/opmi-1.gif
with not much arm and no stand. I think it's missing a lense
from the underside.

I'm temporarily illuminating the Ortholux with the 5V DC from a
spare PC power supply. The lamp says 6 volts, I think. What's
the voltage range from the original rheostat-style power supply?

- John


} }



From daemon Sun Apr 30 09:54:03 2000



From: Vera Shinder :      emshin-at-wis.weizmann.ac.il
Date: Sun, 30 Apr 2000 17:42:37 -1200
Subject: Ion Milling

Contents Retrieved from Microscopy Listserver Archives
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Hi , all!

Does anybody knows something about Ion Milling applications in
biology? Thanks, Vera.



From daemon Sun Apr 30 17:39:28 2000



From: Edsworth-at-aol.com
Date: Sun, 30 Apr 2000 16:49:23 EDT
Subject: Re: kodak digital camera

Contents Retrieved from Microscopy Listserver Archives
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Ron: I've been using the Kodak DS120, which is the camera that is used in the
system you asked about, and have had good results. There are a few things to
keep in mind, however. The DS120 saves images to flash cards using a
propriatary Kodak method that is not recognized by anything else. Therefore,
some of the nifty print systems can't directly use the images. I use the
Kodak-supplied software to copy the images from the flash card to hard disc
via a card reader (SanDisk -- cheap and works real well). Once in my
computer, I work on the images using PhotoShop. Final printing is performed
on my Epson Photo 1200 using print paper. The results are very good.

I do note that when using the DS120 with the MDS adaptor on my Nikon
microscope that there is a bright area in the center. I'm not sure who's
fault that is.

The small viewing screen is a problem, but once the focus is properly set it
is not a major disadvantage. With the system you are talking about
(remember, I just have the camera and adaptor) it may be that the image can
be displayed prior to collecting it onto the flash card.

Overall, I think it's a good value for the money.

If you'd like to talk about the camera, connect off line at edsworth-at-aol.com.

Hope this has helped a little.



From daemon Mon May 01 07:59:58 2000



From: oshel-at-terracom.net (Philip Oshel)
Date: Sun, 30 Apr 2000 17:58:11 -0500
Subject: Re: Freon 113 dehydration

Contents Retrieved from Microscopy Listserver Archives
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Gary,

I'm trying to remember ... Freon 113: Peldri, yes? Or is this one that
stays fluid? (And how did you get it, since Freons are mostly illegal
anymore...) .

First, you might try vacuum, 1 atmosphere or so, maybe not that much,
gently applied, and released.

For Peldri, I put specimens in small vials -- 4 mL Wheatons are my favorite
-- and completely filled the vial, then capped, leaving no air. If there
were enough alcohol:Freon intermediates, and enough changes in pure Freon,
the specimens should be in pure Freon now.

Mind, I mostly did crustaceans and fish bits, not insects with their waxy
epicuticles, but then some or all of that wax is lost in the alcohols
anyway.

But I wouldn't use methanol or acetone. Ethanol works better for insects.
Acetone is OK, but I've found ethanol better, and methanol is too
extractive.

You don't have a CPD for this? Also, some insect parts can be dried from
ethanol or even water. Mandibles of many species for instance, or the
elytra of most beetles. Not whole insects, though.

I never got my specimens to sink in Freon 13, Back When, but they usually
did sink in Peldri.

Phil

} When using F-113, I preceed it with either or both
} methanol and acetone. The curious problem is that
} after either a lengthy immersion in acetone or methanol,
} the specimen (insect) floats when put in F-113. OK.
} So it is lighter than F-113. What is a good method
} of encompassing the specimen with F-113 to further
} dehydrate it prior to vacuum desiccation?
}
} Any ideas?

}}}}}}}}}}}}}}}}}{{{{{{{{{{{{{{{{{
Philip Oshel
Supervisor, AMFSC and BBPIC
Dept. of Animal Health and Biomedical Sciences
University of Wisconsin
1656 Linden Drive
Madison, WI 53706-1581
Voice: (608) 263-4162
peoshel-at-facstaff.wisc.edu
fax: (608) 262-7420 (dept. fax)




From daemon Mon May 01 08:00:41 2000



From: Geoff Williams :      willi1gl-at-cmich.edu
Date: Mon, 01 May 2000 07:54:49 -0400
Subject: Re: Holder Filter

Contents Retrieved from Microscopy Listserver Archives
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Last fall we actually fabricated one of those holders from some copper water
pipe, and purchased the wavy washers from McMasters. I had seen the one Tina
mentions in use and thought "heck, we could make one and save $100." Well we
did just that. For less than $20, if you have a metal cutting saw, some solder
and access to purchasing wavy washers, you can fabricate your own.
One grad student has used it for the spring semester to dehydrate, and CPD,
poly-l-lysine coated 12mm coverslips classifying the morphology and size of
bacteria in the hind gut of Tipula abdomalis. I just reviewed his final work
and the images looked great. He had no problems with debris or samples washing
off.
The advantage of making it yourself is that you can customize the size for the
application for very little money. You would only be limited by the availibity
of sizes of wavy washers.

-Geoff

} } I am searching some device for holding (polycarbonate)filters during
} } dehydration and CPD. I am trying to view mycoplasms with SEM. Has anyone
} } experience with this ?
} }
} We have a device from Tousimis that is designed to hold round glass
} coverslips, and I have used it to hold polycarbonate filters. It is a
} milled-out cylinder open along one side. Wavy washers are used to
} separate the stacked coverslips. There are two sizes, 13 mm and 22 mm,
} part numbers 8766 and 8767. You can contact Tousimis at 1-800-638-9558,
} fax 1-301-881-5374, http://www.tousimis.com
}
} This device works for me! My only affiliation with Tousimis is as a
} satisfied customer.
}
} Aloha,
} Tina
}

--
Geoff Williams

Electron Microscope Facility Supervisor
Biology Department
Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859

Geoffrey.Lloyd.Williams-at-cmich.edu
517 774-3576
517 774-3462 (fax)




From daemon Mon May 01 08:00:42 2000



From: John Foust :      jfoust-at-threedee.com
Date: Mon, 01 May 2000 07:28:11 -0500
Subject: Re: Introductory message

Contents Retrieved from Microscopy Listserver Archives
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Next question about the Ortholux: where might I find the operating
or service manuals?

I'm located in Jefferson, WI, halfway between Madison and Milwaukee.

- John



From daemon Mon May 01 08:20:50 2000



From: Esra SAVAS :      esavas-at-akasya.istanbul.edu.tr
Date: Mon, 1 May 2000 08:12:11 -0500
Subject: Scanning Electron Microscope

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Dear Sir,
I have been working for Istanbul University, Faculty of Fisheries, as a
research Assistant on aquaculture. I am a doctorate student at the
present. My studies are on Aquarium Fish ( ornamental ). The topic of my
doctoral thesis is " A Study on the Artificial Propagation of Discus
Fish ( Symphysodon spp. ) and Effective Factors on the Propagation ".
Besides, I research too my thesis for discus 's embriological-larval
developing and sperm developing with scanning elecron microscope ( SEM
). With regard to my doctoral thesis: Could you send me Scanning
Electron Microscope SEM )' s use-book and example's processing ? I wish
success your studying.
Best regards,

Esra SAVAS
I.U. Su Urunleri Fak.,
Ordu Cad., No: 200 34470
Vezneciler / Istanbul TURKEY




From daemon Mon May 01 12:33:28 2000



From: Susnitzky, David :      david.susnitzky-at-intel.com
Date: Mon, 1 May 2000 07:09:41 -0700
Subject: Announcement - N. California Local Affiliate Meeting on May 11t h

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N. California Society for Microcsopy

MAY 11TH SYMPOSIUM AT U.C. Davis
TOPIC: Electron Backscatter Patterns (EBSP)

Featured Speakers:
1) David Dingley, TSL, "Automated Crystallography for the TEM: Recent
Developments"
2) Adam Schwartz, LLNL, "Coupling Automated Electron Backscatter Diffraction
with Transmission Electron and Atomic Force Microscopies".
3) Patrick Camus, Noran Instruments, "Phase Identification versus Phase
Discrimination"
4) Pierre Rolland, Oxford Instruments, "Mapping Copper Interconnects for the
Semiconductor Industry"
5) John Sutliff, HKL Technology, "Quantifying Polycrystalline
Microstructures: The Automated-ESBP Technique and its Applications"

The symposium will be held in the AGR room in the Buehler Alumni & Visitors
Center, UC Davis

Meeting starts at 3:00
No Host Social -at- 5:30
Dinner Buffet -at- 6:30
Meeting/Dinner will end by 9:00

Dinner Buffet-
Rosemary & Orange Chicken
Fettucine Alfredo
Spring Mix Salad with Raspberry Vinaigrette
Greek Salad topped with Crumbled Feta Cheese on a Bed of Greens
Chef's Selection Vegetable
Fresh Baked Rolls with Butter
New York Cheesecake with Melba Sauce garnished with Mint
Coffee, Decaf, Hot Tea & Iced Water

Cost: $20 regular members, $10 student members, $25 nonmembers

Please call Greg Lum at 415/338-1339 or Email: "glum-at-sfsu.edu" for dinner
reservations by Monday May 8th. The symposium starts at
3:00 p.m. The dinner will start at 6:30 pm.








From daemon Mon May 01 12:33:33 2000



From: George Laing :      scisales-at-ngscorp.com
Date: Mon, 1 May 2000 13:49:27 -0400
Subject: RE: digital cameras for copy stand work?

Contents Retrieved from Microscopy Listserver Archives
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The Nikon D1 Digital SLR camera does not have a mirror lockup but
does have an "Anti Vibration Mode" which causes the shutter opening to
be delayed until mirror shock has subsided.
The D1 has 2.7million pixels, ISO range 200-1600 and accepts
most Nikon lenses, electronic releases,etc. It is excellent for
both copystand and light microscopy applications.

George Laing
National Graphic Supply
226 North Allen Street
Albany, NY 12206
E-mail: scisales-at-ngscorp.com
(800) 223-7130 X3109
(518) 438-8411 X3109

At 08:45 PM 4/28/00 , you wrote:
}
} Just returned from a course on VP SEM (which was excellent) and just now
} can add to some of the digital camera discussion.
}
} One thing not mentioned about using a SLR digital camera like the E1, N60
} is vibration from the mirror and the shutter. If you are going to use a
} camera of this type you must be able to lift the mirror independently,
} like the old F1, whether it's film or CCD. I have a Kodak DCS420 that is
} based on the Nikon N90s camera body and it works ok at low magnifications
} but anything over about 200X just doesn't cut it.
}
}
}




From daemon Mon May 01 13:21:48 2000



From: Maria.Fazio-Zanakis-at-aventis.com
Date: Mon, 1 May 2000 13:02:50 -0500
Subject: unsubscribe

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UNSUBSCRIBE, please.

Maria Fazio-Zanakis
Bioimaging and Molecular Histology
Aventis Pharmaceuticals
1-908-231-3357
Fax: 1-908-231-3962
Email: Maria.Fazio-Zanakis-at-Aventis.com



From daemon Mon May 01 17:18:23 2000



From: Kirk Czymmek :      kirk-at-udel.edu
Date: Mon, 1 May 2000 14:56:56 -0400
Subject: Philips 201 and 501

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I have a Philips 501 SEM and 201 TEM that I am about to dismantle and
discard. They were both operational until four months ago. If anyone needs
them for spare parts please feel free to contact me.


Best Regards,

Kirk J. Czymmek, Ph.D.
Director, Core Microscopy Facility
Department of Biological Sciences
University of Delaware
Newark, DE 19716
kirk-at-udel.edu
PH: (302) 831-1158



From daemon Mon May 01 17:18:35 2000



From: zaluzec-at-aaem.amc.anl.gov
Date: Mon, 1 May 2000 13:35:04 -0500
Subject: PASEM 2000" - THE PRACTICAL ASPECTS SERIES OF SHORT COURSES

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


John,

If you find them let me know I would like a copy. I will pay reasonable
or slightly unreasonable charges for copying and mailing.

Gordon

G. C,. Couger
624 Cheyenne
Stillwater, OK 74075-1411
405 624 2855 between 3:00 pm to 1:00 am CST
405 742 2758 cell
----- Original Message -----
} From: "John Foust" {jfoust-at-threedee.com}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Monday, May 01, 2000 7:28 AM


Meeting: "PASEM 2000" - THE PRACTICAL ASPECTS SERIES OF SHORT COURSES
Dates: May 22 through June 2, 2000
Topic: The Practical Aspects Series consists of intensive 3.5 to 4.5 day
short courses that provide participants with a thorough coverage of the
basic theory and practice of
scanning electron microscopy and associated techniques. Training is
achieved through the use
of easy to understand lectures coordinated with supervised laboratory
sessions. The
laboratory class sizes are purposely kept small so that participants will
have an opportunity to
gain extensive hands- on experience with available SEM and EDS
instrumentation. Designed for the
academic, government and industrial user, the courses are beneficial to
microscopists
and microanalysts at all levels from novice through advanced. Course titles
and dates for our
Year 2000 series follow:

1. SCANNING ELECTRON MICROSCOPY - May 22 through May 26, 2000
2. ADVANCED TOPICS IN SCANNING ELECTRON MICROSCOPY - May 30 through June
2, 2000
3. X-RAY MICROANALYSIS - May 30 through June 2, 2000

Sponsor: University of Maryland
Location: College Park, Maryland, USA
Interests: Both Physical & Biological Sciences
Fields: SEM, EDS
Contact: Tim Maugel, University of Maryland, Department of Biology,
Building 144, Room 0240, College Park, Maryland, 20742, USA
Tel: 301-405-6898
Fax: 301-314-9358
E-mail: tm11-at-umail.umd.edu
WWW: http://www.life.umd.edu/pasem




From daemon Tue May 02 07:26:40 2000



From: varguc-at-freemail.c3.hu
Date: Tue, 2 May 2000 07:17:42 -0500
Subject: EDS spectra on PC

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html





Dear Christian!

If some of your spectra are only available in LINK AN/10000 format,
then you could convert them to simple text files with a little
program I wrote about a year ago.
It's a 32 bit Windows program. You can select as many LINK spectrum
files (in their original, not converted format) as you need. All the
selected files will be converted in one run. I have tried
it with more than 1700 spectra. The result of the conversion is a
text file with the same name but with different file extension. An
example from a converted spectrum file (from a series):
-------------------------------LAK11S103.SP---------------------------
ak11s** 3

preset live time: 40
live time: 40
real time: 49
20.000000 eV/channel

Energy[keV], counts
-0.200000, 0
-0.180000, 0
-0.160000, 0
-0.140000, 0
-0.120000, 3
-0.100000, 16
-0.080000, 42
-0.060000, 98
..
----------------------------------------------------------------------
If you are still interested in a program like this, drop me a line
and I'll send it to you.

With the best regards:
Laszlo Varga


On 20 Apr 00, at 10:21, Filion, Christian wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Good morning,
}
} I have both a Spectra file in Link format and txt format saved as a MSA
} type file. Is there a software or an excel macro that could read these
} spectra ?
}
} Thank you
}
} Christian Filion
} Superviseur Laboratoires
} Aciers Inoxydables Atlas
} 1640 Marie-Victorin
} Tracy, Québec
} J3R 5R5
} Tel : 450-746-5243
} fax : 450-746-5241
}
}




From daemon Tue May 02 07:26:45 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Tue, 2 May 2000 07:14:20 -0500
Subject: Microscopy & Microanalysis 2000 Search Engine Now On-Line

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Colleagues...

For those of you interested the Microscopy and Microanalysis 2000 Meeting
Search Engine is now on line.

Using the Search engine you can find out dates/times/locations
of any author, paper, subject or session at the upcoming meeting this
August in Philadelphia.


Just go to http://www.msa.microscopy.com

and follow the links to the M&M 2000 meeting.

See you in Philly....

Cheers....

Nestor
Your Friendly Neighborhood SysOp




From daemon Tue May 02 15:57:33 2000



From: nessler :      randy-nessler-at-uiowa.edu
Date: Tue, 02 May 2000 08:36:33 -0500
Subject: SEM Peltier stage vendors?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We are looking for vendors of Peltier cooling stages for our Hitachi
2460N VPSEM. If you know of a company, please drop me a line. Also, if
you have opinions on what features you would look for in such an item,
I'd like to hear it.
Thanks in advance,
Randy
--
Randy Nessler
Views expressed are my own.



From daemon Tue May 02 15:57:33 2000



From: Tamara Howard :      howard-at-cshl.org
Date: Tue, 2 May 2000 09:37:46 -0400 (EDT)
Subject: Re: Drive Belt for Pyramitome

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Never replaced a Pyramitome belt - but LKB microtome belts can be replaced
by "regular" belt material - just match the width and springiness (did you
keep any of the old belt fragments?). If your institution doesn't have a
machine shop (those guys usually have extra belts floating around), try
your local hardware store/superstore. I've had good luck replacing
standard parts this way, and it is probably going to be light years
cheaper than an "official" replacement part, if such a thing is available.

} From experience, giant rubber bands do not work very well.........

Tamara Howard
CSHL

On Tue, 2 May 2000 mganger-at-aol.com-at-sparc5.microscopy.com wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Date: Tue, 2 May 2000 08:08:07 EDT
} Subject: Drive Belt for Pyramitome
} To: Microscopy-at-sparc5.microscopy.com
} MIME-Version: 1.0
} Content-Type: text/plain; charset="US-ASCII"
} Content-Transfer-Encoding: 7bit
} X-Mailer: AOL 5.0 for Windows sub 104
}
} Greetings,
}
} I would like to know if anyone out there knows of a repair place that sells
} parts for an LKB 11800 Pyramitome. The drive belt for the specimen arm has
} literally fallen apart and I need to replace it. Any suggestions on where I
} could get one would be greatly appreciated.
}
} Thanks in advance.
}
} Mike Ganger
} Montclair State University
} Montclair, New Jersey
} mganger-at-aol.com
}
}
}
}



From daemon Tue May 02 15:57:37 2000



From: Rick Harris :      raharris-at-ucdavis.edu
Date: Tue, 02 May 2000 10:28:58 -0700
Subject: Re: Drive Belt for Pyramitome

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} I would like to know if anyone out there knows of a repair place that sells
} parts for an LKB 11800 Pyramitome. The drive belt for the specimen arm has
} literally fallen apart and I need to replace it. Any suggestions on where I
} could get one would be greatly appreciated.

Be sure to look at vacuum cleaner drive belts. They come in many sizes and
I've used them as drive belts for a lot of devices. They look like a giant
O-ring, up to 1/4 inch thick and in many lengths.



Rick A. Harris, Director
Microscopy and Imaging Facility
Section of Molecular and Cellular Biology
1241 Life Sciences Addition
University of California
Davis, CA
530 752 2914
530 754 7536 fax
http://katie.ucdavis.edu
raharris-at-ucdavis.edu



From daemon Tue May 02 16:04:29 2000



From: Michael O'Keefe :      MAOKeefe-at-lbl.gov
Date: Tue, 02 May 2000 14:00:35 -0700
Subject: Two places left

Contents Retrieved from Microscopy Listserver Archives
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Ernest Orlando Berkeley National Laboratory
One Cyclotron Road, Berkeley, California 94720
FOR IMMEDIATE RELEASE

Two places left for students to attend the
NCEM Summer School on Computing in Electron Microscopy
June 26-30, 2000 in Berkeley, California


(Berkeley, CA) The seventh annual Summer School on Computer-Interactive
HRTEM Image Acquisition, Processing and Simulation will be held at the
National Center for Electron Microscopy (NCEM), Lawrence Berkeley
National Laboratory, University of California, Berkeley from June 26
through June 30, 1999.

The curriculum will focus on training participants in techniques of
computer-assisted acquisition and interpretation of high-resolution
electron microscope images, including HRTEM image processing and
simulation, electron holography, focal-series reconstruction, and
remote-control microscopy. Participants will learn general principles
and apply them to specific cases. Instruction will focus on the use of
computer assistance rather than microscope training, although
participants will acquire images on NCEM microscopes as well as using
specific application programs for image interpretation. Class size
will be limited to 16. Deadline for applications, May 01, 2000, has
been extended to May 04, 2000..

Participants who wish to apply newly acquired techniques to their own
projects will be encouraged to extend their visit at NCEM into the next
week. Please note: this type of arrangement requires advance submission
of an NCEM microscope proposal (see:
http://ncem.lbl.gov/frames/user.htm). Projects may involve prepared
specimens for microscopy, images and diffraction patterns for
processing, or crystal and defect data for simulations.

For more information and application materials, contact:

Website: http://ncem.lbl.gov/frames/workshops.htm#workshops
email: JLCavlina-at-lbl.gov
Phone: 510/486-6036
Fax: 510/486-5888.


# # # #




From daemon Tue May 02 20:43:12 2000



From: Barbara Foster :      mme-at-map.com
Date: Tue, 02 May 2000 17:57:56 -0400
Subject: LM: Stains for polymers?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

I have been reading a great deal on polymer microscopy lately and came
across the statement that there was very little information to be gained by
staining polymers and (as might be expected) that they did not take up
stain well. Many years ago, I had heard of an application in which osmium
tetroxide was used, I believe on polyethylene. Can anyone shed any light,
either on the general comment or on the application using osmium tetroxide?

Many thanks,
Barbara Foster,President
Microscopy/Microscopy Education
125 Paridon Street Suite 102
Springfield, MA 01118-2130
PH: 413-746-6931 FX: 413-746-9311 email: MME-at-map.com
Website: www.MME-Microscopy.com/education

America's first national consortium of microscopy specialists offering
customized on-site training in all areas of microscopy, image analysis, and
sample preparation
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^



From daemon Tue May 02 20:43:19 2000



From: John J. Bozzola :      bozzola-at-siu.edu
Date: Tue, 2 May 2000 18:58:46 -0500
Subject: dry mounting dye sub prints

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does anyone have a good, safe way of mounting Codonics dye
sublimation prints onto poster boards? We would like to avoid the
spray on "rubber cement" types of glues due to the mess.

Thank you.

####################################################################
John J. Bozzola, Ph.D., Director
Micro-Imaging and Analysis Center
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
####################################################################



From daemon Wed May 03 08:26:49 2000



From: Richard E. Edelmann :      edelmare-at-casmail.muohio.edu
Date: Wed, 3 May 2000 06:39:20 -0500
Subject: Looking for "non-reflective" foil

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Double sided tape or a product that picture framers use that
just the glue without the tape. It is on a tape that releases
the glue. It does an excellent job of mounting. I have pictures
that were mounted with the Scotch brand 20 years ago that
are just as good as the day I did them. It will also stick to
the plastic use in resin coated photographic paper.

Any picture framing shop should have it.

Gordon

Gordon Couger gcouger-at-couger.com

Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00
----- Original Message -----
} From: "John J. Bozzola" {bozzola-at-siu.edu}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Tuesday, May 02, 2000 6:58 PM


O.k., whereas not directly a microscopy question I was hoping some one would
have a solution. Look for something that will function like aluminium foil but be non-
reflective.

(1) shapable around objects (we're covering petri dishes), but not too massive

(2) be opaque (visble light 420-720nm)

(3) be non-reflective on at least one side. "Non-reflective": less than 15%
reflectivity to visible spectrum 420-720nm.


Any suggestions? (Any good ideas for turning Al foil nice and black?)

Thanks.

Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Supervisor
352 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu

"RAM disk is NOT an installation procedure."



From daemon Wed May 03 08:26:49 2000



From: Gary Coulton :      g.coulton-at-ic.ac.uk
Date: Wed, 03 May 2000 12:04:14 +0100
Subject: ICHC 2000

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All,

There is still time to register for:

11th International Congress of histochemistry and Cytochemistry (ICHC 2000)

Cell Biology Tools for the New Century

ICHC 2000 is the premier meeting this year to address the very latest
developments in visualisation techniques for Cell Biology. to be held in
the magical medieval city of York, England between 3-8 September 2000.

We have a fabulous speaker listof over 100 including some of the biggest
names in Cell Biology and Imaging such as Roger Tsien, Richard Haugland,
Fred Bosman, Alan Boyde, Stefan Hell, Alan Fine, Jim Smith, Margaret
Buckingham, Nick White, Angus Lamond, David Becker, Jeniffer
Lipincott-Schwartz and many, many more.

Topics include -Tracking Molecules in Cells, Fluorescent markers of Gene
Expression, Live Cell Imaging in plants and Fungi, Apoptosis, Cell
Signalling, Environmental toxicology, Imaging Embryonic Development, Ion
Imaging in Cells, Imaging in the Neurosciences etc., etc. etc.

See the science and have a chance to talk to the best in the field.

Come for a holiday as well as the science we can offer many great excursions.

For more information and details of how to register please visit our
web-site at

www.med.ic.ac.uk/external/ichc_2000

Student bursaries are still available!

DON'T MISS OUT IT WILL BE ONE OF THE CONFERENCES OF THE YEAR

Gary Coulton

Dr. Gary Coulton
Molecular Pathology
Division of Biomedical Sciences
Imperial College School of Medicine
The Sir Alexander Fleming Building
South Kensington
London SW7 2AZ

tel 0044 (0)171 594 3190
fax 0044 (0)171 594 3022

PLEASE NOTE AS FROM APRIL 1ST 2000 THE FOLLOWING NUMBERS WILL REPLACE THOSE
ABOVE AND SHOULD BE USED.

TEL. ++44 020 7594 3190
FAX. ++44 020 7594 3022
e-mail g.coulton-at-ic.ac.uk


-------------------------------------
Announcing the 11th International Congress of Histochemistry and
Cytochemistry (ICHC 2000)

"Understanding Biocomplexity: The Post-Genome Challenge"

September 3-8, 2000, York, United Kingdom

ICHC 2000 will comprise 27 symposia addressing the latest developments and
applications of histochemistry and cytochemistry in the life sciences
including medicine.

Many leading experts to speak

On-line registration now open!!!!!!!!!!

For further details of the meeting and how to pre-register please visit our
web-site at http://www.med.ic.ac.uk/external/ichc_2000

Hope to see you there.



From daemon Wed May 03 08:37:18 2000



From: Geoff McAuliffe :      mcauliff-at-UMDNJ.EDU
Date: Wed, 03 May 2000 09:22:48 -0400
Subject: Re: Looking for "non-reflective" foil

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


"Richard E. Edelmann" wrote:

} O.k., whereas not directly a microscopy question I was hoping some one would
} have a solution. Look for something that will function like aluminium foil but be non-
} reflective.
}
} (1) shapable around objects (we're covering petri dishes), but not too massive
}
} (2) be opaque (visble light 420-720nm)
}
} (3) be non-reflective on at least one side. "Non-reflective": less than 15%
} reflectivity to visible spectrum 420-720nm.
}
} Any suggestions? (Any good ideas for turning Al foil nice and black?)
}
} Thanks.
}

I seem to remember that studio photographers use flat black aluminum foil. Try Porter's
Camera, I think they are in Iowa. They are a large mail-order firm and probably have a
website. Or try Calumet camera, 800 Supreme Drive, Bensenville, IL 1-800-225-8638.

Geoff
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************




From daemon Wed May 03 08:37:18 2000



From: Walck, Scott D. :      walck-at-ppg.com
Date: Wed, 3 May 2000 09:17:58 -0400
Subject: RE: dry mounting dye sub prints

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The best stuff that I ever found for mounting pictures is 3M Positionable
Mounting Adhesive. This is very good stuff and not messy at all. It comes
in rolls and is a transfer type material. You roll it out, place your
picture on it and cut it out with a sharp knife. You then press the
adhesive with the backing sheet and peel off the sheet. The adhesive is
transferred to every square inch of the print. You can position this stuff
around and then you press it down hard and it sticks forever.
The roll that I use is PMA 568. There is larger and smaller sizes
available. I think that you have to order through a photographic supply
house because I don't think any of the EM supply houses carry it.


-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)



} -----Original Message-----
} From: John J. Bozzola [mailto:bozzola-at-siu.edu]
} Sent: Tuesday, May 02, 2000 7:59 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: dry mounting dye sub prints
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListse} rver/FAQ.html
}
}
} --------------------------------------------------------------
} ---------.
}
}
} Does anyone have a good, safe way of mounting Codonics dye
} sublimation prints onto poster boards? We would like to avoid the
} spray on "rubber cement" types of glues due to the mess.
}
} Thank you.
}
} ####################################################################
} John J. Bozzola, Ph.D., Director
} Micro-Imaging and Analysis Center
} 750 Communications Drive - MC 4402
} Southern Illinois University
} Carbondale, IL 62901 U.S.A.
} Phone: 618-453-3730
} Fax: 618-453-2665
} Email: bozzola-at-siu.edu
} Web: http://www.siu.edu/departments/shops/cem.html
} ####################################################################
}



From daemon Wed May 03 08:37:18 2000



From: Tamara Howard :      howard-at-cshl.org
Date: Wed, 3 May 2000 09:25:09 -0400 (EDT)
Subject: Re: Looking for "non-reflective" foil

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Matte black spray paint?

Tamara Howard
CSHL

On Wed, 3 May 2000, Richard E. Edelmann wrote:

}
} O.k., whereas not directly a microscopy question I was hoping some one would
} have a solution. Look for something that will function like aluminium foil but be non-
} reflective.
}
} (1) shapable around objects (we're covering petri dishes), but not too massive
}
} (2) be opaque (visble light 420-720nm)
}
} (3) be non-reflective on at least one side. "Non-reflective": less than 15%
} reflectivity to visible spectrum 420-720nm.
}
}
} Any suggestions? (Any good ideas for turning Al foil nice and black?)
}
} Thanks.
}
} Richard E. Edelmann, Ph.D.
} Electron Microscopy Facility Supervisor
} 352 Pearson Hall
} Miami University, Oxford, OH 45056
} Ph: 513.529.5712 Fax: 513.529.4243
} E-mail: edelmare-at-muohio.edu
}
} "RAM disk is NOT an installation procedure."
}
}



From daemon Wed May 03 18:40:50 2000



From: Tobias Baskin :      BaskinT-at-missouri.edu
Date: Wed, 3 May 2000 08:48:54 -0500
Subject: Re: LM: Stains for polymers?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


As to pyramitomes, if anyone has one that is not in use and would like to sell it, please contact me. I used one years ago and wouldn't mind having one around.
Debby


Debby Sherman, Manager Phone: 765-494-6666
Microscopy Center in Agriculture FAX: 765-494-5896
Dept. of Botany & Plant Pathology E-mail: sherman-at-btny.purdue.edu
Purdue University
1057 Whistler Building
West Lafayette, IN 47907-1057

--------------------------------------




Greetings,
Babara Foster asked about staining polymers. By chance, I
recently looked at paper on staining polymers for electron
microscopy. The paper compared osmium and ruthenium tetroxides and
concluded that ruthenenium tet stained many more polymers than did
the osmium. This paper has a kind of encylopedic feel to it because
they checked a lot of polymers. The citation is Trent et al., 1983
Macromolecules 16: 589-598. I have no idea whether this would work
with light microscopy or what info you could get from it exactly
besides contrast, but perhaps you will find the paper interesting.

As ever,
Tobias
}
} Hi,
}
} I have been reading a great deal on polymer microscopy lately and came
} across the statement that there was very little information to be gained by
} staining polymers and (as might be expected) that they did not take up
} stain well. Many years ago, I had heard of an application in which osmium
} tetroxide was used, I believe on polyethylene. Can anyone shed any light,
} either on the general comment or on the application using osmium tetroxide?
}
} Many thanks,
} Barbara Foster,President
} Microscopy/Microscopy Education
} 125 Paridon Street Suite 102
} Springfield, MA 01118-2130
} PH: 413-746-6931 FX: 413-746-9311 email: MME-at-map.com
} Website: www.MME-Microscopy.com/education
}
} America's first national consortium of microscopy specialists offering
} customized on-site training in all areas of microscopy, image analysis, and
} sample preparation
} ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^

_ ____ ^ __ ____ Tobias I. Baskin
/ \ / / \ / \ \ University of Missouri
/ | / / \ \ \ Biological Sciences
/___/ /__ /___ \ \ \__ 109 Tucker Hall
/ / / \ \ \ Columbia, MO 65211-7400 USA
/ / / \ \ \ voice: 573-882-0173
/ /____ / \ \__/ \____ fax: 573-882-0123



From daemon Wed May 03 18:40:50 2000



From: Tobias Baskin :      BaskinT-at-missouri.edu
Date: Wed, 3 May 2000 08:59:33 -0500
Subject: Re: Looking for "non-reflective" foil

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,
We got a bolt of black cloth from a theatrical supply house.
It is the kind of thing used to make curtains and other masking on
the stage. I think it was called "covert black" but I could have that
wrong. We were doing photobiological work and were forever having to
limit the pesky wandering photons. The cloth is thick but not too
thick to wrap around a dish. It is not perfectly opaque but if you
need that I bet you could line the cloth with al foil, on the inside.
Hope this helps,
Tobias
}
}
} O.k., whereas not directly a microscopy question I was hoping
} some one would
} have a solution. Look for something that will function like
} aluminium foil but be non-
} reflective.
}
} (1) shapable around objects (we're covering petri dishes),
} but not too massive
}
} (2) be opaque (visble light 420-720nm)
}
} (3) be non-reflective on at least one side.
} "Non-reflective": less than 15%
} reflectivity to visible spectrum 420-720nm.
}
}
} Any suggestions? (Any good ideas for turning Al foil nice and black?)
}
} Thanks.
}
} Richard E. Edelmann, Ph.D.
} Electron Microscopy Facility Supervisor
} 352 Pearson Hall
} Miami University, Oxford, OH 45056
} Ph: 513.529.5712 Fax: 513.529.4243
} E-mail: edelmare-at-muohio.edu
}
} "RAM disk is NOT an installation procedure."

_ ____ ^ __ ____ Tobias I. Baskin
/ \ / / \ / \ \ University of Missouri
/ | / / \ \ \ Biological Sciences
/___/ /__ /___ \ \ \__ 109 Tucker Hall
/ / / \ \ \ Columbia, MO 65211-7400 USA
/ / / \ \ \ voice: 573-882-0173
/ /____ / \ \__/ \____ fax: 573-882-0123



From daemon Wed May 03 18:40:51 2000



From: wft03-at-health.state.ny.us
Date: Wed, 3 May 2000 10:12:38 -0400
Subject: Re: Looking for "non-reflective" foil

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html





O.k., whereas not directly a microscopy question I was hoping some one
would
have a solution. Look for something that will function like aluminium foil
but be non-
reflective.

(1) shapable around objects (we're covering petri dishes), but not too
massive

(2) be opaque (visble light 420-720nm)

(3) be non-reflective on at least one side. "Non-reflective": less
than 15%
reflectivity to visible spectrum 420-720nm.


Any suggestions? (Any good ideas for turning Al foil nice and black?)




Dear Richard,

How about depositing carbon black onto Al foil? It's messy, but
fulfills

your requirements. Evaporating carbon seems like an inferior proceedure,
but

that should also work. Just holding the foil above a low-temp flame
(candle or

bunsen burner with the air turned down) seems the simplest. You can then

cover the carbon layer with something if necessary. Dipping the foil into
laser-

printer toner could also work, and the toner could then be heated. Putting
the

foil (cut to 8.5" x 11") through the printer is asking for trouble, but
that could

also work if you can find a solid black character--WordPerfect 5.1 has such

a character, which can be accessed by ctrl-v, 3,3. Good luck.

Yours,

Bill Tivol




From daemon Wed May 03 18:40:54 2000



From: Neal Leddy :      nleddy-at-tcd.ie
Date: Wed, 3 May 2000 15:39:55 +0100
Subject: TEM: Immunogold labelling protocol

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,

I am looking for a protocol for Immunogold labelling membrane proteins on
mitochondria / peroxisomes fractions..., I am hoping to use a formaldehyde
type fixation..............to LR white resin, I would appreciate any advice,
references, and if possible actual relevant protocols. Thank you in
advance.

Neal Leddy nleddy-at-tcd.ie



From daemon Wed May 03 18:40:56 2000



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Wed, 03 May 2000 08:52:31 -0700
Subject: Re: dry mounting dye sub prints

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear John,
Years ago I purchased "Mounting Film" from an EM supply house. This is
double-sided sticky tape, two feet wide. You cut it to any size and press
on. Mine is made by Lomacoll and is obviously German.
At 06:58 PM 5/2/00 -0500, you wrote:
}
} Does anyone have a good, safe way of mounting Codonics dye
} sublimation prints onto poster boards? We would like to avoid the
} spray on "rubber cement" types of glues due to the mess.
}
} Thank you.
}
Best regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Wed May 03 18:40:56 2000



From: Heather A Owen :      owenha-at-csd.uwm.edu
Date: Wed, 3 May 2000 11:13:31 -0500 (CDT)
Subject: Re:Mounting Dye-Subs

Contents Retrieved from Microscopy Listserver Archives
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We've used DryBond Adhesive Pads for mounting dye-sublimation prints.
It's manufactured by Chartpak (their part number is DBS25 for 25 11" x 17"
sheets). I order it from EMS (Cat. #77612-25), described on Page 311 of
EMS Catalog XIII. Basically it consists of an array of tiny (well,
actually quite large to electron microscopists) dots of rubber cement like
stuff that you apply by placing the print on top, covering with a
protective sheet then rubbing with a rubber brayer. Peel up the print,
place on mounting board, cover with sheet and rub with brayer. We've
used it on color dye-subs with and without the XtraLife coating with no
problems. As far as longevity, I made a poster two years ago that is
still adhered tight to the board, but that was with RC B&W paper. The
oldest dye-sub has only been observed for about a year now.

I have no financial interest in Chartpak or EMS, the stuff is just nice to
use.

Heather Owen

Heather A. Owen, Director
Electron Microscope Laboratory
Department of Biological Sciences
University of Wisconsin - Milwaukee
(414)229-6816




From daemon Wed May 03 18:40:59 2000



From: Damian :      dneuberger-at-mindspring.com
Date: Wed, 03 May 2000 13:00:17 -0500
Subject: Re: dry mounting dye sub prints

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


John,

I use a product called Liquid Paper Dryline Permanent Adhesive made by the
Gillette Company. It is supposedly acid free, glues instantly, no drying
time, and comes as either permanent or temporary. I get it a office supply
store.



} Does anyone have a good, safe way of mounting Codonics dye sublimation
} prints onto poster boards? We would like to avoid the spray on "rubber
} cement" types of glues due to the mess.

Damian Neuberger, Ph.D.
Baxter Healthcare, Inc.
WG3-2S
P.O. Box 490
Round Lake, IL 60073
e-mail: damian_neuberger-at-baxter.co,
Tel: 847-270-5888
Fax: 847-270-5897



From daemon Wed May 03 18:41:03 2000



From: Smartech :      smartech-at-javanet.com
Date: Wed, 3 May 2000 15:23:00 -0400
Subject: Digital Light microscopy, Kodak MDS 120, How does it compare?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I want to buy an inspection scope from which I can also acquire digital
images.

I remembered that I had a flyer for the Kodak MDS 120 system ($1895).

I called Kodak, they still sell the same system. It seems a little "out
dated" now w/ only 1.2 pixels and no USB connection.

Are there other product that offer similar simplicity and versatility
(macroscopic off the scope capability), that also fall in the same price
range, and have 2 million pixels w/ a USB connection?

Thanks in Advance

Ric



From daemon Wed May 03 18:41:09 2000



From: Stuart McKernan :      mckernan-at-cems.umn.edu
Date: Wed, 3 May 2000 16:20:39 -0500 (CDT)
Subject: MICROSCOPY AND MICROANALYSIS 2000

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Now that the program for the meeting is available on the website - Thanks Nestor
- I would like to advertize the "LATE-BREAKING POSTER SESSION AT MICROSCOPY AND
MICROANALYSIS 2000" for any remaining authors (or potential authors) who would
like to contribute to the meeting.

Microscopy and Microanalysis 2000 will feature a poster session composed of
presentations of newly acquired data or analyses which were unavailable for
submission by the February 15 deadline. A short, half page abstract describing
the studies is required. The abstract should include: Title, Authors, Authors
affiliation, and a Brief Description of the studies. The description should
include the Aim of the studies, a short characterization of the Methods, and a
brief account of the Results and their Importance.

Abstracts should be e-mailed or faxed to the program chair, Stuart McKernan, at
stuartm-at-tc.umn.edu (email) or 612-625-5368 (fax). Abstracts may be submitted
immediately but must be received by June 23, 2000. Abstracts will be reviewed by
members of the program committee. A limited number of poster boards are
available and preference will be given to early submissions. Abstract authors
will be notified of acceptance of their abstracts no later than July 1 (earlier
for early submissions).



__________________
Stuart McKernan stuartm-at-tc.umn.edu
Director Office:(612) 626-7594
IT Characterization Facility, University of Minnesota Desk:(612) 624-6009
100 Union Street S. E., Minneapolis, MN 55455 NEW-} Fax:(612) 625-5368



From daemon Wed May 03 19:17:49 2000



From: Barry Searle :      B.Searle-at-unsw.edu.au
Date: Wed, 3 May 2000 18:42:02 -0500
Subject: JEOL COATER

Contents Retrieved from Microscopy Listserver Archives
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Can someone on the listserver offer any help, I am seeking the JEOL
publication of the list of O Rings (part numbers, or size/type) that are
used in the JEOL COATER JEE-400 and the JEE 4B/4C. If it is available
could you please either email me a copy of Fax me a copy Fax Number is
(in australia) +61 (2) 9385 6400. Thankyou for your help, Barry EM
UNIT UNSW




From daemon Wed May 03 19:17:49 2000



From: Tom Doman :      jtd1-at-psu.edu
Date: Wed, 3 May 2000 18:48:01 -0500
Subject: virus particle concentration

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




My question is : I need to obtain a estimate of virus particle
concentration in a sample of cell culture supernatant for an article to be
submitted to a journal. I was told of a method called the 'latex particle
reference method' for obtaining virus particle counts. Can anyone on the
list supply information for this procedures ?
Thanks for your time.

Sincerely,

Tom Doman, Project Associate
Veterinary Science Department
Penn State University




From daemon Thu May 04 07:58:12 2000



From: Marilyn Henderson :      marilyn.henderson-at-adelaide.edu.au
Date: Thu, 04 May 2000 10:20:43 +0930
Subject: Re: TEM: Immunogold labelling protocol

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Neal,
A good fixative for immunogold work is still a combination of glut. +
paraformaldehyde, but using a 'low' glutaraldehyde concentration. One
you could try would be 0.25% glutaraldehyde + 4% paraformaldehyde in
phosphate buffered saline pH 7.2. Formaldehyde alone results in poor
structural preservation.
From there on you could follow the instructions for dehydration,
infiltration etc. from the LR White pamphlet which works well.
For labelling:
1. Pre-treat with 0.02M glycine in PBS to block any free aldehyde groups
(2x10mins), 2. Block with a suitable protein, e.g. BSA, ovalbuimin (1%-
1X20mins)
3. Place each grid on a 15-20ul drop of primary antibody, suitably
diluted, and either leave overnight at 4 degrees C in fridge, or on lab
bench for about 2 hours.
4. Rinse 6 times in drops of PBS with 1% protein block (BSA etc.) -
(6X5mins).
5. Place each grid on a 20ul drop of Protein-A Gold complex, suitably
diluted, for 1-1.5 hours.
6. Rinse in PBS (6X5mins).
7. Wash in distilled or millipore filtered water (in small beakers, 3 x
several rapid dips(about 6), making sure the grids remain under the
water at all times during dipping).
8. Stain with Uranyl Acetate and Lead Citrate.

** Blot (side-on) onto filter paper between steps 1+2, 2+3, 4+5, 7+8.

Good luck.

Cheers,
Marilyn

Neal Leddy wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hello,
}
} I am looking for a protocol for Immunogold labelling membrane proteins on
} mitochondria / peroxisomes fractions..., I am hoping to use a formaldehyde
} type fixation..............to LR white resin, I would appreciate any advice,
} references, and if possible actual relevant protocols. Thank you in
} advance.
}
} Neal Leddy nleddy-at-tcd.ie



From daemon Thu May 04 07:58:15 2000



From: Jeff & Wanda Gray :      jefwan-at-flash.net
Date: Wed, 3 May 2000 21:02:31 -0500
Subject: Job Opening at Univ of Houston

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Please contact the person at the end of the post, not me, as I am posting at
the request of someone without access. Thank you.
----- Original Message -----
Sent: Wednesday, May 03, 2000 8:59 PM


} JOB OPORTUNITY AT UNIVERSITY OF HOUSTON
}
}
} Supervisor: Histology and Microscopy Laboratory; College of Optometry;
} University of Houston, Texas
}
} Job Description:
}
} This position is in direct support of research and teaching faculty
} where the subject of interest is typically ocular and neural tissue. The
} duties range from tissue preparation, embedment, sectioning and
} mounting, to operation of light and transmission electron microscopes,
} and development and printing of photographic results. Knowledge and
} competency in operation of ultramicrotomes and the transmission electron
} microscope as well as current techniques in morphology, histology, and
} immunocytochemistry is required. Experience with cryo-microscopy, in
} situ hybridization, and computer image analysis is desirable. The
} successful candidate also may participate as co-author of research
} papers describing research performed in the laboratory.
}
} The supervisor is responsible for maintenance of stocks of laboratory
} supplies and care of the equipment, and keeping the lab clean and
} usable, including proper disposal procedures for hazardous wastes. Other
} duties include instructing graduate students in common and specialized
} anatomical techniques required for their various projects, and
} supervision and consultation on their work as required. Some effort also
} is applied to teaching of undergraduate optometry students including
} preparation of teaching slides and technical instruction in anatomical
} methods.
}
} This job is scheduled to begin on 1 May 1999. Salary will be negotiated
} based upon qualifications and experience. For further information, you
} may contact:
}
} Chris Kuether, Technical Services Manager
} College Of Optometry, University Of Houston
} 4901 Calhoun Blvd. Houston TX 77204-6052
} vox:(713)743-2049 fax:--2053; ckuether-at-uh.edu
}
}
}



From daemon Thu May 04 07:58:20 2000



From: jim :      jim-at-proscitech.com.au
Date: Thu, 4 May 2000 14:01:16 +1000
Subject: RE: SEM Peltier stage vendors?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


You can read up on Emitech cold stages on our site
home} contents} K4
We are agents for Emitech for Australasia only (south of Singapore), just hope
that you would find the info useful.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Tuesday, May 02, 2000 11:37 PM, nessler [SMTP:randy-nessler-at-uiowa.edu]
wrote:

} We are looking for vendors of Peltier cooling stages for our Hitachi
} 2460N VPSEM. If you know of a company, please drop me a line. Also, if
} you have opinions on what features you would look for in such an item,
} I'd like to hear it.
} Thanks in advance,
} Randy
} --
} Randy Nessler
} Views expressed are my own.



From daemon Thu May 04 07:58:38 2000



From: Scott Whittaker :      sdw-at-biotech.ufl.edu
Date: Thu, 04 May 2000 08:28:27 -0400
Subject: Re: virus particle concentration

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have archived three discussions on this from the listserver. Go to:

http://www.biotech.ufl.edu/~emcl

and look in the TEM section of "Tips & Tricks" under virus. It also has a
few other ideas. Good luck


At 06:48 PM 5/3/2000 -0500, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America





} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {
GO GATORS
Scott D. Whittaker 218 Carr Hall
EM Technician Gainesville, FL 32610
University Of Florida ph 352-392-1184
ICBR EM Core Lab fax 352-846-0251
sdw-at-biotech.ufl.edu http://www.biotech.ufl.edu/~emcl/
The home of " Tips & Tricks "







From daemon Thu May 04 07:58:38 2000



From: Matt Ervin :      mervin-at-ARL.mil
Date: Thu, 4 May 2000 08:37:54 -0400
Subject: Looking for "non-reflective" foil

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I think that I have used something that would work well for your
application. It is an anodized Al foil that we use for laser safety
purposes. The foil is very flat black and the black does not rub off
easily. However, the foil is significantly stiffer than household Al foil.
The brand we have used is BlackwrapTM and it can be purchased from The
Great American Market, Hollywood CA 323-461-0200. They have a web site as
well. I hope that this helps.

Matthew Ervin, Ph.D.
U.S. Army Research Laboratory
(301)394-0017
MErvin-at-ARL.mil




"Richard E. Edelmann" {edelmare-at-casmail.muohio.edu} on 05/03/2000 07:39:20
AM

Please respond to Edelmare-at-muohio.edu

To: microscopy-at-sparc5.microscopy.com
cc:


O.k., whereas not directly a microscopy question I was hoping some one
would
have a solution. Look for something that will function like aluminium foil
but be non-
reflective.

(1) shapable around objects (we're covering petri dishes), but not too
massive

(2) be opaque (visble light 420-720nm)

(3) be non-reflective on at least one side. "Non-reflective": less
than 15%
reflectivity to visible spectrum 420-720nm.


Any suggestions? (Any good ideas for turning Al foil nice and black?)

Thanks.

Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Supervisor
352 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu

"RAM disk is NOT an installation procedure."









From daemon Thu May 04 08:10:55 2000



From: Rosemary Walsh :      rw9-at-psu.edu
Date: Thu, 4 May 2000 07:59:21 -0500
Subject: Sony DKC-5000 CatsEye Digital Camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers,
Does anyone currently use the Sony DKC-5000 CatsEye Digital Camera
and if
so would you recommend it for a shared technology lab.
Rosemary Walsh, EM Facility for the Life Sciences
Penn State University




From daemon Thu May 04 10:12:35 2000



From: Huggins, Bradley J :      HUGGINBJ-at-bp.com
Date: Thu, 4 May 2000 08:17:30 -0500
Subject: RE: Stains for polymers?

Contents Retrieved from Microscopy Listserver Archives
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Barbara,
We use both osmium and ruthenium tetroxide to stain various polymers for EM
observation. Ruthenium is less selective in its staining, and therefore
does stain many more polymers. Both compounds stain unsaturated polymers
pretty well. In olefins, like polyethylene, RuO4 is good for staining
amorphous domains within the crystalline or semi-crystalline polymer fine
structure. This is typically a TEM issue. With some rather coarse polymer
blends (e.g. rubber inclusions in another polymer matrix) RuO4 or OsO4 can
be used with SEM and FESEM imaging. I would expect to see very little
staining contrast by LM due to the polymer domain sizes of typical
co-polymers, polymer blends, or crystalline/amorphous polymer structure.
Again the best reference I have is the book by L. Sawyer, Polymer
Microscopy.

Brad Huggins
BP Amoco, Naperville, IL

} ----------
} From: Barbara Foster[SMTP:mme-at-map.com]
} Sent: Tuesday, May 02, 2000 4:57 PM
} To: Confocal Microscopy List; microscopy-at-sparc5.microscopy.com
} Subject: LM: Stains for polymers?
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi,
}
} I have been reading a great deal on polymer microscopy lately and came
} across the statement that there was very little information to be gained
} by
} staining polymers and (as might be expected) that they did not take up
} stain well. Many years ago, I had heard of an application in which osmium
} tetroxide was used, I believe on polyethylene. Can anyone shed any
} light,
} either on the general comment or on the application using osmium
} tetroxide?
}
} Many thanks,
} Barbara Foster,President
} Microscopy/Microscopy Education
} 125 Paridon Street Suite 102
} Springfield, MA 01118-2130
} PH: 413-746-6931 FX: 413-746-9311 email: MME-at-map.com
} Website: www.MME-Microscopy.com/education
}
} America's first national consortium of microscopy specialists offering
} customized on-site training in all areas of microscopy, image analysis,
} and
} sample preparation
} ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
} ^^
}



From daemon Thu May 04 10:12:37 2000



From: David Kinast :      DKinast-at-hitschfel.com
Date: Thu, 04 May 2000 09:29:04 -0500
Subject: Re: Digital Light microscopy, Kodak MDS 120, How does it

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Tom,

We use routinely this method in our laboratory. Here is the used protocol of
quantitation of virus particles by negative stain electron microscopy:


A selected volume (100 uL) of supernatant containing virus particles is
mixed with a selected volume (100 uL) of polystyrene latex beads of known
concentration (about 10e8 beads by mL) and a size between 100 - 200 nm in
diameter.
The mixture is placed in an Beckman Airfuge 240 µL-tube. A Formvar and
carbon-coated grid is inserted into the bottom of the microtubes.* The tubes
are placed in a Airfuge A-100 fixed angle rotor (30¡) and centrifuge at 20
psi (120 000 g) for 5 minutes.
The grids are recovered with fine self-closing tweezers, dried with bibulous
paper, stained 1 minute with phosphotungstic acid (PTA 3%, pH6.0) and dried
again with bibulous paper. Samples are visualized under a transmission
electron microscope with an approppriate magnification.
On two different grids, 200-500 particles (latex beads or virus particles)
are counted from at least five different areas on each grid. That way, from
the ratio of the two types of particles in the suspension, the ratio of the
volumes added and the known concentration of latex particles, the
concentration of viral particles can be calculated.

Virus particle concentration (particles/mL) = (virus count / latex beads
count) X (latex beads concentration) X (1/test article dilution)


The level of sensitivity of this procedure is between 10e6 and 10e10
particles per mL. Less than 10e6, there is not enough virus to get a
realistic count and more than 10e10, there are too much virus to well
differentiate them.
This procedure is used principally to quantify Retrovirus type-A and -C
particles in cells supernatant, but can be used for any virus particles.

*R. Alain et al, J.Virol. Meths, 16 (1987), 209-216
*Alain, R. Microscopy Today, May 1997, issue#97-4, D. Grimes Ed, p. 20

If you need more explanation you can contact us. If you are not the
equipment to do this technique, we can offer this service at low price.

Robert Alain

**********************************************************
Robert Alain, M.Sc.
Microscopie Žlectronique

INRS-Institut Armand-Frappier
Centre de Microbiologie et Biotechnologie
531 boul. des Prairies
Laval, QuŽbec
CANADA H7V 4B7
Tel: (450)687-5010 ext#4388
Fax: (450)686-5626
e-mail: Robert.alain-at-INRS-iaf.uquebec.ca
or Robert_alain-at-hotmail.com
Http://www.iaf.uquebec.ca/iaf/recherche/viro/me.html
**********************************************************

----- Original Message -----
} From: Tom Doman {jtd1-at-psu.edu}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Wednesday, May 03, 2000 7:48 PM


{html}
You may want to look at the Olympus DP-11 digital camera. You can find it
on their web page at:
{a href="http:///"} http://www.olympusamerica.com/product.asp?c=21&s=11&p=18&product=612 {/a} {br}
It has very high resolution, live video output (which is quite useful at
low magnification as a focusing and framing aid) and features a c-mount
so that it can be used on any microscope, or with a macro lens.  It
uses SmartMedia cards as the recording media, and combined with a USB
SmartMedia card reader, download to the computer is fast and easy. {br}
{br}
Configured with an AC adapter, 64MB SmartMedia card, USB card reader and
a 9" Sony video monitor, it is priced at $4,349.00.  This is
more than you indicated you would like to spend, but I think that you
will find that its features, resolution and ease of use may justify the
additional expense. {br}
{br}
At 03:23 PM 5/3/00 -0400, you wrote: {br}
>------------------------------------------------------------------------ {br}
>The Microscopy ListServer -- Sponsor: The Microscopy Society of
America {br}

{/html}



From daemon Thu May 04 16:31:00 2000



From: Richard E. Edelmann :      edelmare-at-casmail.muohio.edu
Date: Thu, 4 May 2000 11:35:05 -0500
Subject: Thanks on the non-reflective and EELS humor

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thanks to everyone who responded - I was thinking anodizing myself but Matt
Ervin actually had an Off-the-Shelf source.

===============

Its exam time here and I wanted to share this answer from my EM Theory Final:

Ques: What does "EELS" stand for? What does it detect (Be specific!)?

Ansr: Energized Eucaliptic Leaf Shooter. If properly used it can be used to lure the
better part of the world's Koala Bear population in a general area to get a more
precise population density reading.

[The student recieved 1 point for creativity]



Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Supervisor
352 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu

"RAM disk is NOT an installation procedure."



From daemon Thu May 04 16:31:01 2000



From: David Kinast :      DKinast-at-hitschfel.com
Date: Thu, 04 May 2000 08:39:20 -0500
Subject: LM product specialist needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


{html}
{br}
{br}
{font size=6} {b} {div align="center"}
SALES POSITION AVAILABLE {br}
{br}
{br}
{/font} {/b} {font size=5} {/div}
Hitschfel Instruments, Incorporated is a consultative sales company,
providing microscope based imaging solutions to the biomedical research,
clinical and industrial communities within the states of Missouri,
Nebraska, Kansas and Oklahoma plus central & southern Illinois. {br}
{br}
We are seeking one exceptional and highly motivated individual to work
with us toward our mutual goals of success through customer
satisfaction.Ê A strong science background coupled with experience in the
fields of microscopy and digital-imaging technology is required.Ê If you
are the right person for the job, we will teach you the selling skills
that you will need to be successful. {br}
{br}
If you are looking for a lifelong career in scientific instrument sales,
please submit your resume to: {br}
{br}
{div align="center"}
Hitschfel Instruments, Incorporated {br}
2333 South Hanley Road {br}
St. Louis, MO 63144 {br}
Attn: David Kinast {br}
FAX: 314/644-5877 {br}
{/font} {font size=4 color="#0000FF"} {u} dkinast-at-hitschfel.com {br}
{a href="http://www.hitschfel.com/" eudora="autourl"} www.hitschfel.com {br}
{/a} {/font} {/u}
{BR}
{div} David L. Kinast {/div}
{div} Hitschfel Instruments, Inc. {/div}
{div} 2333 South Hanley Rd. {/div}
{div} St. Louis, MO 63144 {/div}
{div} Phone: {x-tab}    {/x-tab} 800/242-3501 {/div}
{div} Phone: {x-tab}    {/x-tab} 314/644-6660 {/div}
{div} Fax: {x-tab}      {/x-tab} {x-tab}     {/x-tab} 314/644-5877 {/div}
{div}
{a href="http://www.hitschfel.com/" EUDORA=AUTOURL} www.hitschfel.com {/a} {/div}
{div} dkinast-at-hitschfel.com {/div}
{/html}

{/html}



From daemon Thu May 04 16:31:04 2000



From: Sarah Lundberg :      lundberg-at-nevada.edu
Date: Thu, 04 May 2000 11:10:20 -0700
Subject: CCL Supplier Information Request

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello listers-
I am hoping that someone might have a current address or phone for
"Cambride Image Technologies Ltd." or "Nuclide" A colleague of mine is
looking to purchase a cold cathode luminoscope and was given these two
names. Or if someone knows of another supplier any information would be
appreciated.
Thank you,
Sarah

************************************************************************

Sarah A.W. Lundberg Lab
(EPMA) (702) 895-2660 or
Electron Microanalysis and
(SEM) (702) 895-2462
Imaging Laboratory
Office (702) 895-1134
Department of Geoscience, UNLV Fax
(702) 895-4064
4505 S. Maryland Parkway Box 454010
Las Vegas, NV 89154-4010 Dept Office
(702) 895-3262

lundberg-at-nevada.edu
http://www.unlv.edu/Colleges/Sciences/Geoscience/EMP.htm

Home:
8025 W. Russell Rd. Apt. #1117
Las Vegas, NV 89113
(702) 871-9635
************************************************************************





From daemon Thu May 04 16:31:04 2000



From: Larry :      mishot-at-itsa.ucsf.edu
Date: Thu, 04 May 2000 11:17:05 -0700
Subject: "non-reflective" foil

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I believe that what Geoff was referring to is called "Black Wrap." It is a
heavy foil with a matte black coating. It is used by theater lighting
people worldwide to create custom masks and light shields. It is made by
several companies. My local lighting house sells black wrap made by Great
American ... in 12 inch by 50 foot or 24 inch by 25 foot rolls for
US$27.50. Since you're at a university you could probably go over to the
theater department and get a little piece to try.

Larry


Larry D. Ackerman
Lily & Yuh Nung Jan Laboratories
Howard Hughes Medical Institute
UCSF, Box 0725, Rm U226
533 Parnassus Ave.
San Francisco, CA 94143

(415) 476-8751 FAX (415) 476-5774
mishot-at-itsa.ucsf.edu



From daemon Thu May 04 16:31:08 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Thu, 04 May 2000 14:19:48 -0700
Subject: Re: Sony DKC-5000 CatsEye Digital Camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 05:59 AM 5/4/00 , you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

After some time of getting used to it, the 5000 is OK. It uses
a small CCD so the image files are small and not really high
resolution. But for relatively modest final image output size,
it is a good camera. It provides real time focusing via a
separate RGB+sync color monitor. However, adjusting the
monitor to match the captured image's exposure is tricky.

If you are going to be moving the camera around, remember
that it uses a SCSI interface to download captured images.
You won't be able to move it very far from the SCSI port
unless you use a notebook or laptop computer.

gary g.



From daemon Thu May 04 21:48:35 2000



From: Gordon Couger :      gcouger-at-rfdata.net
Date: Thu, 4 May 2000 19:28:11 -0500
Subject: Mounting tape for posters

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Scotch 924 Transfer Tape 1/2 inch wide is the product I mentioned
earlier. It cost about 6.95 a roll for a life time supply for many of us.
http://productinfo.mmm.com/us/office/products/office.jhtml?powurl=4JLD1SMBbe
ZCZYKRQ9geT1T4S9TCgvPDJGVQ33gl

The way I used it to mount photos was stick a band of it around the
edge of the photo as close as I could to the edge. That's all I did
for 5X7's for 8X10 I put a cross diagonally from corner to corner.
I didn't mount prints larger than that but If I had I would have added
some tape in the open areas. Some one made a small version
that had an applicator but the refills cost as much as a full sized roll
and it is not much if any easier to use.

I tried a lot of things spray glue, two sided tissue and a number of
others. This was the easiest and most consistent other than Seal
mount tissue and a hot press.

The hardware store where I drink coffee is will sell mail order. I have
not interest in the hardware store other than a bunch of us drink their
coffee. Their web page address is www.studyboards.com or 405 372 2644
ask of Sandy or A.J.

Gordon

Gordon Couger gcouger-at-couger.com

Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00




From daemon Fri May 05 07:45:33 2000



From: Udo Graf :      udo.graf-at-bal-tec.com
Date: Fri, 5 May 2000 08:38:51 +0200
Subject: WG: SEM Peltier stage vendors?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



You can find information on www.bal-tec.com --} products--} vct100

Best regards,
Udo Graf
BAL-TEC AG
+423 388 12 26
+423 388 12 60 (Fax)
udo.graf-at-bal-tec.com



+ -----Ursprüngliche Nachricht-----
+ Von: nessler [mailto:randy-nessler-at-uiowa.edu]
+ Gesendet: Dienstag, 2. Mai 2000 14:37
+ An: miscroscopy listserver
+ Betreff: SEM Peltier stage vendors?
+
+
+ We are looking for vendors of Peltier cooling stages
+ for our Hitachi
+ 2460N VPSEM. If you know of a company, please drop me a
+ line. Also, if
+ you have opinions on what features you would look for in
+ such an item,
+ I'd like to hear it.
+ Thanks in advance,
+ Randy
+ --
+ Randy Nessler
+ Views expressed are my own.
+



From daemon Sat May 06 20:54:25 2000



From: Tim E. Harper :      tim-at-cmp-cientifica.com
Date: Fri, 5 May 2000 17:09:03 +0200
Subject: Fossil Replicas for SEM inspection

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

I have customer here requiring some help. They are making replicas of fossil
mandibles for SEM analysis. Their problem is how to localise the areas of
interest when the specimen is in the SEM. I was sent the quotation below,
and we are trying to find out whether we, or anyone else, can supply the
sort of material they need. My background is mainly materials, so I'm
farming this out to the list to see if anyone has any suggestions, or maybe
even recognises the fragment below.


"Fiberglas screening material:
fiber thickness (310 microns), hole width (1,
240 microns), and length (1,340 microns), wiped with Permabond 910 adhesive
and pressed onto the surface so that contact was made everywhere".


Thanks in advance

Tim

*****************************************************************
Tim E. Harper Managing Director
CMP Cientifica s.l.
Space & NanoTechnology Division
Phone +34 91 640 71 85 Fax +34 91 640 71 86
http://www.cmp-cientifica.com/ {http://www.cmp-cientifica.com/}



From daemon Sat May 06 20:54:27 2000



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Fri, 05 May 2000 11:20:44 -0500
Subject: Re: Fossil Replicas for SEM inspection

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


That sounds a lot like fiberglass window screen to me, but the holes here
are probably a bit smaller. There is also a fiberglass screening used for
backing plaster repairs. It might have a pitch more similar to what you
described.

At 05:09 PM 5/5/2000 +0200, you wrote:
} Hi,
}
} I have customer here requiring some help. They are making replicas of fossil
} mandibles for SEM analysis. Their problem is how to localise the areas of
} interest when the specimen is in the SEM. I was sent the quotation below,
} and we are trying to find out whether we, or anyone else, can supply the
} sort of material they need. My background is mainly materials, so I'm
} farming this out to the list to see if anyone has any suggestions, or maybe
} even recognises the fragment below.
}
}
} "Fiberglas screening material:
} fiber thickness (310 microns), hole width (1,
} 240 microns), and length (1,340 microns), wiped with Permabond 910 adhesive
} and pressed onto the surface so that contact was made everywhere".
}
}
} Thanks in advance
}
} Tim
}
} *****************************************************************
} Tim E. Harper Managing Director
} CMP Cientifica s.l.
} Space & NanoTechnology Division
} Phone +34 91 640 71 85 Fax +34 91 640 71 86
} http://www.cmp-cientifica.com/ {http://www.cmp-cientifica.com/}



From daemon Sat May 06 20:54:27 2000



From: Larry :      mishot-at-itsa.ucsf.edu
Date: Fri, 05 May 2000 09:57:37 -0700
Subject: Re: Sony DKC-5000 CatsEye Digital Camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have had the Sony DKC-5000 for quite a few years and hve never had a
problem with the hardware despite many clumsy users. We have it running on
a Mac computer taking the Sony RGB signal to SVHS via a Harmonic Research
CV-233P video encoder. The SVHS signal is input to a ATI Xclaim video board
in the Mac. So the live window and the Sony acquire plug in run on the same
monitor. The density and color balance rarely coincide between the two
displays. We have had many problems with users changing settings and adding
software, etc. on the Mac resulting in problems with the video display. As
Dr Gaugler said, the resolution is not very high. There should be better
systems now but I continue to recommend that our scientists record their
images on color slide film and then scan it with a $2000 slide scanner
(Nikon, Polaroid etc.) much cheaper, higher resolution and bandwidth and
easy convenient storage!





Larry D. Ackerman
Lily & Yuh Nung Jan Laboratories
Howard Hughes Medical Institute
UCSF, Box 0725, Rm U226
533 Parnassus Ave.
San Francisco, CA 94143

(415) 476-8751 FAX (415) 476-5774
mishot-at-itsa.ucsf.edu



From daemon Sat May 06 20:54:28 2000



From: Huggins, Bradley J :      HUGGINBJ-at-bp.com
Date: Fri, 5 May 2000 12:22:45 -0500
Subject: low temperature microscopy of synthetic fluids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



We are still not set-up well for Cryo-SEM, and although we have made some
progress and learned much about these techniques from you all on this
Listserver and others, I am still looking for a "cryomicroscopy facility"
who has, for example, an E-SEM with Peltier Stage/controlled cooling rate
capabilities and with cryostage (capable down to temperatures as low as
approximately -75C); who would be interested in working together with one of
my clients, in a large Chemical/Oil Company, to investigate/observe the
low-temperature structures of various Synthetic Fluids.

Anyone interested, please contact:
Brad Huggins at
BPAmoco, Naperville, IL
hugginbj-at-bp.com
630 420-3668



From daemon Sat May 06 20:54:43 2000



From: Kristen Lennon :      kalen-at-citrus.ucr.edu
Date: Fri, 05 May 2000 15:46:56 -0700
Subject: silver enhancement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All,
Does anyone have a good protocol for silver enhancement of immunogold
labeled sections for TEM? I have a paper in front of me, but they used a
kit. We do light level silver enhancement without a kit all of the time,
but I don't want to chance trying to adapt that method before consulting
all of you.
Thanks for your help!
Have a wonderful weekend,
Kristen
Kristen A. Lennon
Cell, Molecular & Developmental Biology Group
Department of Botany & Plant Sciences
University of California
Riverside, CA 92521
kalen-at-citrus.ucr.edu
909-787-4525



From daemon Sat May 06 20:54:45 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 05 May 2000 15:53:50 -0700
Subject: Re: Sony DKC-5000 CatsEye Digital Camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


If I understand correctly how you are using the DKC-5000,
then you are actually obtaining half the image resolution
that the system can provide.

The DKC-5000 has a 1/2" 440,000 pixel CCD with an
effective image area of 795x598 pixels (RGB). The
actual captured image area is probably closer to 740x580
pixels (RGB). Thus, if my calculations are correct, you
should obtain RGB TIF files which are about 1.29MB in
size.

The image processor in the DKC-5000 digital processor system
unit expands (interpolates?) the original image to one that is
1520x1144 pixels (RGB). This results in a TIF file that is
about 5.21MB in size. However, this resolution is only
obtained via the SCSI interface on the back of the
system box. This is likely why you are obtaining low
resolution results. This is effectively taking a consumer
grade RGB video camera and performing an RS-170
frame grab. This will produce up to 800x600 pixels (RGB)
maximum. This is OK, but the higher cost of the DKC-5000
was due, I think, to the ability to obtain higher quality
images and to do so without any separate/extra image
capture hardware. The DKC-5000 was about $14K I think
versus about $800 for a really nice RGB video camera
with close to 800x600 pixels resolution.

Therefore, if you have any sort of typical Mac, it should have
a legacy SCSI-I connector on the rear. This is easily
connected to the digital processor box. Then, load the
Photoshop plug-in and acquire the higher resolution images
using Photoshop and the SCSI bus. You should still be
able to use the RGB outputs for framing and focusing.
Just don't use the RGB outputs for image capture.

Have you tried this?

gary g.



At 09:57 AM 5/5/00 , you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Mon May 08 23:09:30 2000



From: A. Greene :      ablue-at-io.com
Date: Sun, 7 May 2000 12:15:37 -0500
Subject: Help finding parts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greetings Listers, I am looking for a couple decomissioned electron
microscopes to be used as a source for parts. I would greatly appreciate
any information or leads. The instruments are the Hitachi 7000 TEM and the
Hitachi S-510 (or 515 or 520) SEM. Thank you very much, Alex Greene
SCIENTIFIC INSTRUMENTATION SERVICES, INC.
PMB-499, 1807 West Slaughter Lane, Number 200
Austin, Texas 78748-6200
Phone: 512/282-5507 Fax: 512/280-0702 QUALITY ELECTRON MICROSCOPE REPAIR




From daemon Mon May 08 23:09:30 2000



From: Steve Chapman :      PROTRAIN-at-CompuServe.COM
Date: Sun, 7 May 2000 13:16:29 -0400
Subject: Mike Hales Contact Required

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi

Mike I need to contact you very urgent.

Could you mail me back please?

Steve Chapman
Senior Consultant Protrain



From daemon Mon May 08 23:09:32 2000



From: Gordon Couger :      gcouger-at-rfdata.net
Date: Sun, 7 May 2000 05:06:02 -0700
Subject: Help with very old Leitz projection microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At http://www.couger.com/gcouger/leitz/ are some pictures of a
very old Leitz projection microscope. For it's age and rough
construction it works extreemly well. It is of cast iorn, steel
and brass construction and carries no serial number. It
has two objective lenses that are about 6 and 12 x It has
a simple condenser in the lamp housing and every thing
is adjustable on the stand and the objectives have a heilical
ajustment.

I have as several musem curators and infivigules that have
a life long assoitions wiht Leitz. No one has seen anything
like it. It appears to be all origianl except for the light bulb,
its mounting and the cord. They look like they came from
the 1920's

} From my decussions with Leitz sholers and deduction I think
this was made about the same time as the electric light bulb.
It would be possible that it use a gas or lime light but I don't
think so. The mechenism for centering a light bulb just looks
like it was designed for a bulb.

Any help is welcome. If anyone knows when Leitz started putting
serial numbers on their products it would be a great help in
dating it.

Thanks
Gordon Couger
Stillwater, OK 74075
405 624 2855 GMT - 6:00
www.couger.com/gcouger



From daemon Mon May 08 23:09:37 2000



From: Gordon Couger :      gcouger-at-rfdata.net
Date: Sun, 7 May 2000 09:53:23 -0700
Subject: Re: Help with very old Leitz projection microscope (with corrected spelling)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am sorry the last post some how escaped my spell check
on my email program. It is set to check every email. But it
misses some. For Microsoft an occasional miss is pretty good:)

At http://www.couger.com/gcouger/leitz/ are some pictures of a
very old Leitz projection microscope. For it's age and rough
construction it works extremely well. It is of cast iron, steel
and brass construction and carries no serial number. It
has two objective lenses that are about 6 and 12 x It has
a simple condenser in the lamp housing and every thing
is adjustable on the stand and the objectives have a helical
adjustment.

I have as several museum curators and individuals that have
a life long association with Leitz. No one has seen anything
like it. It appears to be all original except for the light bulb,
its mounting and the cord. They look like they came from
the 1920's

} From my decisions with Leitz scholars and deduction I think
this was made about the same time as the electric light bulb.
It would be possible that it use a gas or lime light but I don't
think so. The mechanism for centering a light bulb just looks
like it was designed for a bulb.

Any help is welcome. If anyone knows when Leitz started putting
serial numbers on their products it would be a great help in
dating it.

Thanks
Gordon Couger
Stillwater, OK 74075
405 624 2855 GMT - 6:00
www.couger.com/gcouger






From daemon Mon May 08 23:09:49 2000



From: Matt Ervin :      mervin-at-ARL.mil
Date: Mon, 8 May 2000 08:49:33 -0400
Subject: CCL Supplier Information Request

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Sarah-
I can't help you with Cambridge Image Technologies Ltd., but I do know
about Nuclide. Nuclide went bankrupt several years ago and was bought up
and reborn as Premier American Technologies Corp. which continued making
the luminoscopes among other things. I worked for PATCO for a couple of
years. I don't know the details, but PATCO has since evolved into Spectru
Medix and I imagine they still sell luminoscopes as it was probably their
most consistent seller in the past. You can call Spectru Medix at
814-867-8600, they are at 2124 Old Gatesburg Rd., State College, PA. I
would suggest you talk to Mike Vollero as I know he still works there and
will be able to put you in contact with the proper people. I hope this
helps.
Matt Ervin




Sarah Lundberg {lundberg-at-nevada.edu} on 05/04/2000 02:10:20 PM

To: MSA listserver {Microscopy-at-sparc5.microscopy.com}
cc:


Hello listers-
I am hoping that someone might have a current address or phone for
"Cambride Image Technologies Ltd." or "Nuclide" A colleague of mine is
looking to purchase a cold cathode luminoscope and was given these two
names. Or if someone knows of another supplier any information would be
appreciated.
Thank you,
Sarah

************************************************************************

Sarah A.W. Lundberg Lab
(EPMA) (702) 895-2660 or
Electron Microanalysis and
(SEM) (702) 895-2462
Imaging Laboratory
Office (702) 895-1134
Department of Geoscience, UNLV Fax
(702) 895-4064
4505 S. Maryland Parkway Box 454010
Las Vegas, NV 89154-4010 Dept Office
(702) 895-3262

lundberg-at-nevada.edu
http://www.unlv.edu/Colleges/Sciences/Geoscience/EMP.htm

Home:
8025 W. Russell Rd. Apt. #1117
Las Vegas, NV 89113
(702) 871-9635
************************************************************************










From daemon Mon May 08 23:10:04 2000



From: Harry J. Grier :      harry.grier-at-dep.state.fl.us
Date: 5/8/00
Subject: Staining Spurr's resin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



We have recently encountered some perplexing problems staining tissue
embedded in Spurr's resin with uranyl acetate and calcined lead. The
tissue is not taking up the stain although there is some stain
precipitate on the sections. Any suggestions?

Sincerely,
Harry Grier
Stock Enhancement Research Facility
Florida Marine Research Institute
14495 Harllee Road
Palmetto, FL 34221
harry.grier-at-fwc.state.fl.us



From daemon Mon May 08 23:10:05 2000



From: Larry :      mishot-at-itsa.ucsf.edu
Date: Mon, 08 May 2000 11:24:09 -0700
Subject: Re: Sony DKC-5000 CatsEye Digital Camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html
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Apparently I was not clear in my description of our DKC-5000 setup. The RGB
signal displayed in a live ATI video window is for focusing and composing.
Acqusitition of the image is via the Sony plug-in and SCSI transfer. The
resulting image file is about 5MB. It does not meet my standards for a
publication quality image.
Larry D. Ackerman
Lily & Yuh Nung Jan Laboratories
Howard Hughes Medical Institute
UCSF, Box 0725, Rm U226
533 Parnassus Ave.
San Francisco, CA 94143

(415) 476-8751 FAX (415) 476-5774
mishot-at-itsa.ucsf.edu



From daemon Mon May 08 23:10:06 2000



From: Gary Radice :      gradice-at-richmond.edu
Date: Mon, 8 May 2000 15:10:46 -0400
Subject: sputter coater maintenance

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have a question about how best to maintain a sputter coater that is used
infrequently.

We are a small college with correspondingly small EM facility. Our SEM and
prep equipment may go for several weeks without being used, then someone
will need it heavily for a class for three or four weeks. Invariably we
find that our sputter coater seems to be the weak link in our plans, since
it rarely works well when we fire it up after long periods of dormancy. I
understand this is common with vacuum equipment: better to use it often
rather than shut it off.

Am I correct that we should we have a plan to regularly pump down the
sputter coater, and if that is good idea, how often should that be? Every
day? Once a week? Once a month?

Or, if we don't need to pump it down regularly, are there other things we
should be doing do it the down time instead of letting it just sit there?

and finally, are some designs better able to handle long periods of disuse?
Do we just have the wrong sputter coater?

Gary P. Radice gradice-at-richmond.edu
Associate Professor of Biology 804 289 8107 (voice)
University of Richmond 804 289 8233 (FAX)
Richmond VA 23173 http://www.science.richmond.edu/~radice




From daemon Mon May 08 23:10:07 2000



From: Philip Oshel :      peoshel-at-facstaff.wisc.edu
Date: Mon, 8 May 2000 15:56:43 -0500
Subject: See-Vac Inc.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does anyone have any old parts for the See-Vac, Inc. Autoconductavac
sputter coaters? Especially the power feed-through that connects
through the cap to the target. Thanks.

Phil
--
}}}}}}}}}}}}}}}}}{{{{{{{{{{{{{{{{{
Philip Oshel
Supervisor, AMFSC and BBPIC
Dept. of Animal Health and Biomedical Sciences
University of Wisconsin
1656 Linden Drive
Madison, WI 53706-1581
voice: (608) 263-4162
fax: (608) 262-7420 (dept. fax)



From daemon Mon May 08 23:10:08 2000



From: Chere Petty :      cpetty1-at-umbc.edu
Date: Mon, 08 May 2000 17:11:44 -0400
Subject: Job Announcement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Microscopist: To manage light and electron microscopy facility. Minimal
degree requirement BS (MS preferred). Experience with confocal
microscopy, computerized image analysis and histological sample
preparation essential. Experience in SEM/TEM sample preparation and
video microscopy desirable. Must be an interactive person willing to
facilitate microscopy experiments for faculty and students with a wide
variety of interests in a University setting. Salary commensurate with
experience. Full time desired but will consider part time. For further
information on the department see http://www.umbc.edu/biosci UMBC is an
AA/EOE.

Contact: Dr. Daphne Blumberg, Chair, Microscopist Search Committee,
Dept. of Biological Sciences, University of Maryland Baltimore County
(UMBC), Baltimore MD 21250.




From daemon Tue May 09 00:20:30 2000



From: jim :      jim-at-proscitech.com.au
Date: Tue, 9 May 2000 14:09:53 +1000
Subject: RE: sputter coater maintenance

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


All vacuum systems are better "stored" under vacuum, with the exception of any
systems that cannot vent the rotary pump when power is off. Those systems may
suck oil from the pump towards the vacuum chamber.

The more common problem for infrequently used systems is moisture in the pump
oil. Particularly in moist climates and when relatively short pumping times are
employed a good deal of water is absorbed in the pump. When the pump is not
used for a lengthy period this may cause corrosion and certainly lowers the
vapour pressure of the contaminated oil and so lower performance results.

I suggest that at the end of your spasmodic activities the pump should be run
with the baffle valve partially open for at least 30 minutes. The baffle valve
is usually atop the rotary pump. You could also use the sputter coater's needle
valve, partially open. Under these conditions the pump will run hotter and
throw-out a good deal of oil mist and water vapour. Vent the exhaust into a
fume hood since oil mist is not just unpleasant. You will find that the pump
performs much better after a baffle run.

SEM and TEM usually do not need this treatment, but they may, for instance if a
TEM has been used to "dry" film that was not previously dried in another
system.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Tuesday, May 09, 2000 5:11 AM, Gary Radice [SMTP:gradice-at-richmond.edu]
wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I have a question about how best to maintain a sputter coater that is used
} infrequently.
}
} We are a small college with correspondingly small EM facility. Our SEM and
} prep equipment may go for several weeks without being used, then someone
} will need it heavily for a class for three or four weeks. Invariably we
} find that our sputter coater seems to be the weak link in our plans, since
} it rarely works well when we fire it up after long periods of dormancy. I
} understand this is common with vacuum equipment: better to use it often
} rather than shut it off.
}
} Am I correct that we should we have a plan to regularly pump down the
} sputter coater, and if that is good idea, how often should that be? Every
} day? Once a week? Once a month?
}
} Or, if we don't need to pump it down regularly, are there other things we
} should be doing do it the down time instead of letting it just sit there?
}
} and finally, are some designs better able to handle long periods of disuse?
} Do we just have the wrong sputter coater?
}
} Gary P. Radice gradice-at-richmond.edu
} Associate Professor of Biology 804 289 8107 (voice)
} University of Richmond 804 289 8233 (FAX)
} Richmond VA 23173 http://www.science.richmond.edu/~radice
}
}



From daemon Tue May 09 07:20:55 2000



From: Udo Graf :      udo.graf-at-bal-tec.com
Date: Tue, 9 May 2000 09:01:53 +0200
Subject: AW: low temperature microscopy of synthetic fluids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The new BAL-TEC 'VCT 100' Vacuum-Cryo-Transfer-Equipment is a modular system
consisting of:

- Shuttle (Cryo-Vacuum Conditions)
- Docking station at any preparation system
- Docking station at any analysis system (SEM, ESEM, Cryo-AFM)
- Cryo equipment for any analysis system (SEM, ESEM)

The modules can be arranged just to meet your needs.

VCT 100 Cryo equipment has been adapted to a Philips XL30 e.g.

Additional information you will find on www.bal-tec.com --} products--} vct100

Best regards,
Udo Graf
BAL-TEC AG
+423 388 12 26
+423 388 12 60 (Fax)
udo.graf-at-bal-tec.com



-----Ursprüngliche Nachricht-----
Von: Huggins, Bradley J [mailto:HUGGINBJ-at-bp.com]
Gesendet: Freitag, 5. Mai 2000 19:23
An: Huggins, Brad; Microscopy listserver
Betreff: low temperature microscopy of synthetic fluids


------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America



We are still not set-up well for Cryo-SEM, and although we have made some
progress and learned much about these techniques from you all on this
Listserver and others, I am still looking for a "cryomicroscopy facility"
who has, for example, an E-SEM with Peltier Stage/controlled cooling rate
capabilities and with cryostage (capable down to temperatures as low as
approximately -75C); who would be interested in working together with one of
my clients, in a large Chemical/Oil Company, to investigate/observe the
low-temperature structures of various Synthetic Fluids.

Anyone interested, please contact:
Brad Huggins at
BPAmoco, Naperville, IL
hugginbj-at-bp.com
630 420-3668



From daemon Tue May 09 07:20:59 2000



From: Matt Ervin :      mervin-at-ARL.mil
Date: Tue, 9 May 2000 08:04:36 -0400
Subject: sputter coater maintenance

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Gary-
I am not sure if you are saying that your sputter coater is pumping
down poorly or that it is depositing poor quality films. In either case,
it sounds like poor vacuum is the problem. Water vapor adsorbing to the
deposition chamber walls over a period of disuse will pump off of the walls
very slowly. A "wet" chamber can take 10 times as long to pump down to
ultimate vacuum as a "dry" one. If this sounds like it might be causing
the symptoms you are seeing, I would suggest you try one of two things:

1. Place a valve between the sputter chamber and the pump. Valve off the
chamber and leave it under vacuum between uses. As long as it retains any
vacuum this will help when you try to use it again. You don't want to be
pumping on the chamber at the pump's ultimate vacuum for any extended
period of time because that will allow oil vapor from the mechanical pump
to backstream into your sputter chamber and possibly onto your specimen if
present! (I am assuming that you have an oil based pump.) It is also nice
to spare the pump the wear and tear of pumping continuously over periods of
disuse.

OR
2. Several hours or the day before you want to use it, start pumping on the
sputter chamber while admitting a small flow of argon. The argon is an
important part of this procedure. First of all, the argon will prevent the
backstreaming described above. Second, I have been told that the argon
helps to desorb the water from the chamber surfaces. I don't know if that
is an old wives tale or not, but it does seem reasonable. The argon flow
may also help in purging any condensed vapors from the pump's oil. Don't
use too much argon though or you may overheat your pump.

I hope that this addresses the problem you are experiencing.
Matt Ervin
(301)394-0017
U.S. Army Research Laboratory
Adelphi MD




Gary Radice {gradice-at-richmond.edu} on 05/08/2000 03:10:46 PM

To: Microscopy-at-sparc5.microscopy.com
cc:


I have a question about how best to maintain a sputter coater that is used
infrequently.

We are a small college with correspondingly small EM facility. Our SEM and
prep equipment may go for several weeks without being used, then someone
will need it heavily for a class for three or four weeks. Invariably we
find that our sputter coater seems to be the weak link in our plans, since
it rarely works well when we fire it up after long periods of dormancy. I
understand this is common with vacuum equipment: better to use it often
rather than shut it off.

Am I correct that we should we have a plan to regularly pump down the
sputter coater, and if that is good idea, how often should that be? Every
day? Once a week? Once a month?

Or, if we don't need to pump it down regularly, are there other things we
should be doing do it the down time instead of letting it just sit there?

and finally, are some designs better able to handle long periods of disuse?
Do we just have the wrong sputter coater?

Gary P. Radice gradice-at-richmond.edu
Associate Professor of Biology 804 289 8107 (voice)
University of Richmond 804 289 8233 (FAX)
Richmond VA 23173 http://www.science.richmond.edu/~radice









From daemon Tue May 09 08:10:16 2000



From: White, Woody N :      Woody.N.White-at-mcdermott.com
Date: Tue, 9 May 2000 08:15:00 -0500
Subject: Re:RE: sputter coater maintenance

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I agree. In addition...
Given a choice, I would always use the process gas to purge - using the
"leak
valve" to feed a partial pressure to the system until the vacuum pump heats
up.
Opening the ballast valve will help (lowers ultimate vacuum when open) purge
the
pump, but IMHO, it is better to use dry gas than atmosphere.

Woody White
McDermott Technology

{SNIP}
I suggest that at the end of your spasmodic activities the pump should be
run
with the baffle valve partially open for at least 30 minutes. The baffle
valve
is usually atop the rotary pump. You could also use the sputter coater's
needle
valve, partially open.



From daemon Tue May 09 08:10:16 2000



From: Gary Radice :      gradice-at-richmond.edu
Date: Tue, 9 May 2000 08:47:12 -0400
Subject: answers to my sputter coater problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thanks to all who responded to my query about how best to maintain my
infrequently used sputter coater. Since some have replied off list and
others have asked to see all the responses I thought I would append all of
the responses here, (without direct attribution, since strictly speaking I
don't have all the authors permission to do this....I hope they understand).

Thanks to all who responded. Based on the comments, I think my problem was
accumulation of water in the oil and vacuum surfaces, and dried out
gaskets. Greasing the gaskets and running the pump longer got the pump-down
time from 20 minutes to 3 minutes. Keeping the chamber under vacuum isn't
practical with our coater design, but I can probably solve my problem by
arranging to pump down the chamber once a week, paying attention to keeping
the seals lightly greased, and changing the pump oil on a regular schedule.


******************

} Hi Gary,
}
} How are you? Our sputter coater has sat for over a year at a time without
} use. It had no problems when it was started. However, that is not an
} ideal situation. Vacuum equipment whould be regularly pumped down to
} out-gas the chamber, etc. We have a Denton Desk II Sputter Coater. It is
} by far the best I have used. It is now used regulary because we have a new
} SEM. If I were you I would pump down the chamber on the coater at least
} once a month. Change the oil in the pumps once/year. Check the seal
} between the chamber and the base and the glass and the seal before the
} class use starts. Use fomblin grease or some other non-hydrocarbon vacuum
} grease.
*************

} There really should not be any heroics needed in order to snap your figers
} and have the sputter coater work well. We ship our coaters all over the
} world to trade shows, open up the boxes, take them out, put them on the
} table, and a few minutes later we are coating samples for prospective
} customers. And I think that would probably be the case for most
} commercially made coaters today that are used in the SEM market.
}
} But I will tell you one thing that does happen and that is that the needle
} valve can develop a "set" if it is left tightened down real tight over long
} periods of time.
}
} The when you go to use it again, because of the "set", there are problems
} controlling its action and therefore the bleed rate of air or inert gas. I
} know that conventional wisdom says a vacuum system should be stored "under
} vacuum" but the typical coater is sufficiently leaky, that the vacuum is
} going to disappear shortly anyhow.
}
} So you might want to try storing it no under vacuum, that is, with the need
} valve open, and see if that does not make your problems disappear.

******************

} Hello Dr. Radice,
} Yes, the worst thing you can do to a vacuum system is to not use it. The
} least you should do is keep the bell jar under vacuum when it is not being
} used. Do you vent with Dry Nitrogen or room air? All high voltage leads
} and feed throughs should be kept very clean since they have a tendency to
} collect Carbon and crud. It might not be a bad idea to pre-run your high
} voltage up rather high (higher than you would normally use it), pror to a
} run. This should stabilize things a bit.

***********

} Gary,
} After many years of using sputter coaters I have found that it is best
} that they are kept under vacuum all the time. Many sputter coaters do
} not let you maintain a vacuum when they are off. With this type I have
} found that I have to keep the glass cylinder and the metal target very
} clean and allow plenty of pump down time when first using the unit after
} prolonged shut down. Also any solvent based glues that may be used,
} silver dag or colloidal carbon, must be dry before putting the sample in
} the coater, at least 2 hours after mounting.
**************

} I have a similar use pattern as yours, but I have never had any problems
} with my coater. Sometimes, our coater my sit for a period of months before
} a pump down is required. Back in 1993 I purchased an Emitech, the same
} type that is sold through EMS today, and have had but only one failure.
} That failure was due to foil on a circut board that was not heavy enough to
} handle the current required by the vacuum pump. The foil had melted, but
} was an easy fix for me. Otherwise I have had no failures.
}
} It certainly can help to pump it down once a week, just like it helps
} ink-jet printers to print a test page once a week. Other than keeping
} non-contaminated oil in the pump, a light coating of vacuum grease on the 0
} rings to keep them from drying out and getting attacked by ozone, I have
} not had other maintenance issues. Perhaps your vacuum problems are related
} more to your pump and the need for some maintenance and oil change, perhaps
} your seals need replacing, or perhaps your coater is going through a period
} where it requires higher than normal maintenance. I'm curious, what type
} of problems are you having?
}
} Good luck with your facility. I always enjoy meeting others who are
} running small EM labs.
*******************

} All vacuum systems are better "stored" under vacuum, with the exception of
} any
} systems that cannot vent the rotary pump when power is off. Those systems may
} suck oil from the pump towards the vacuum chamber.
}
} The more common problem for infrequently used systems is moisture in the pump
} oil. Particularly in moist climates and when relatively short pumping
} times are
} employed a good deal of water is absorbed in the pump. When the pump is not
} used for a lengthy period this may cause corrosion and certainly lowers the
} vapour pressure of the contaminated oil and so lower performance results.
}
} I suggest that at the end of your spasmodic activities the pump should be run
} with the baffle valve partially open for at least 30 minutes. The baffle
} valve
} is usually atop the rotary pump. You could also use the sputter coater's
} needle
} valve, partially open. Under these conditions the pump will run hotter and
} throw-out a good deal of oil mist and water vapour. Vent the exhaust into a
} fume hood since oil mist is not just unpleasant. You will find that the pump
} performs much better after a baffle run.
}
} SEM and TEM usually do not need this treatment, but they may, for instance
} if a
} TEM has been used to "dry" film that was not previously dried in another
} system.
*************

} We used to leave the sputter coater sitting for weeks after pumping it down
} and never had any problems. It sounds as if you may have a leak at your
} sealing surface. We always had to be very careful about cleanliness of the
} bell jars surface and the plate it seals on. A small grain of sand or other
} material can fracture the glass so that you have leaks and requires
} repolishing of the glass bell jar.
}
} I hope that this helps you.
***************

} Gary-
} I am not sure if you are saying that your sputter coater is pumping
} down poorly or that it is depositing poor quality films. In either case,
} it sounds like poor vacuum is the problem. Water vapor adsorbing to the
} deposition chamber walls over a period of disuse will pump off of the walls
} very slowly. A "wet" chamber can take 10 times as long to pump down to
} ultimate vacuum as a "dry" one. If this sounds like it might be causing
} the symptoms you are seeing, I would suggest you try one of two things:
}
} 1. Place a valve between the sputter chamber and the pump. Valve off the
} chamber and leave it under vacuum between uses. As long as it retains any
} vacuum this will help when you try to use it again. You don't want to be
} pumping on the chamber at the pump's ultimate vacuum for any extended
} period of time because that will allow oil vapor from the mechanical pump
} to backstream into your sputter chamber and possibly onto your specimen if
} present! (I am assuming that you have an oil based pump.) It is also nice
} to spare the pump the wear and tear of pumping continuously over periods of
} disuse.
}
} OR
} 2. Several hours or the day before you want to use it, start pumping on the
} sputter chamber while admitting a small flow of argon. The argon is an
} important part of this procedure. First of all, the argon will prevent the
} backstreaming described above. Second, I have been told that the argon
} helps to desorb the water from the chamber surfaces. I don't know if that
} is an old wives tale or not, but it does seem reasonable. The argon flow
} may also help in purging any condensed vapors from the pump's oil. Don't
} use too much argon though or you may overheat your pump.
******************

} While I have done no experimental protocol to prove this schedule is
} optimum, during the off season I try to remember to run the sputter
} coaters overnight one night a week. This keeps things in good shape. I
} have read that the slow pumpdown of unused vacuum systems is caused mostly
} by water vapor adsorbed on interior surfaces, and by traces of moisture in
} the pump oil.


Gary P. Radice gradice-at-richmond.edu
Associate Professor of Biology 804 289 8107 (voice)
University of Richmond 804 289 8233 (FAX)
Richmond VA 23173 http://www.science.richmond.edu/~radice




From daemon Tue May 09 10:59:58 2000



From: Edward J. King :      king-at-biology.utah.edu
Date: Tue, 09 May 2000 09:45:53 -0600
Subject: EM Technician Position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


This position is available at the University of Utah. Dr. Erik Jorgensen
is the contact person.

________________________________________

Electron Microscopy Lab Technician

Experience in electron microscopy and a Bachelor's degree in a science
or
related field required. Degree in a biological science, experience in
light
microscopy, and familiarity with microcomputers preferred. Operates
electron microscopes, prepares specimens for microscopy, produces
photographic and digital micrographs, analyzes data and maintains
accurate
work records. Necessary training will be provided. Applicants must
submit a
University of Utah Application for Employment.


Erik M. Jorgensen, Ph.D.
Assistant Professor
Department of Biology
University of Utah
257 South 1400 East
Salt Lake City, UT 84112-0840
PHN: (801) 585-3517
FAX: (801) 581-4668
jorgensen-at-biology.utah.edu



From daemon Tue May 09 10:59:58 2000



From: Edward J. King :      king-at-biology.utah.edu
Date: Tue, 09 May 2000 09:51:15 -0600
Subject: EM Technician Position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


This position is available at the University of Utah. Dr. Erik Jorgensen
is the contact person.

________________________________________

Electron Microscopy Lab Technician

Experience in electron microscopy and a Bachelor's degree in a science
or
related field required. Degree in a biological science, experience in
light
microscopy, and familiarity with microcomputers preferred. Operates
electron microscopes, prepares specimens for microscopy, produces
photographic and digital micrographs, analyzes data and maintains
accurate
work records. Necessary training will be provided. Applicants must
submit a
University of Utah Application for Employment.


Erik M. Jorgensen, Ph.D.
Assistant Professor
Department of Biology
University of Utah
257 South 1400 East
Salt Lake City, UT 84112-0840
PHN: (801) 585-3517
FAX: (801) 581-4668
jorgensen-at-biology.utah.edu



From daemon Tue May 09 11:59:48 2000



From: John J. Bozzola :      bozzola-at-siu.edu
Date: Tue, 9 May 2000 11:52:50 -0500
Subject: Re: sputter coater maintenance

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Gary,

Yes, we find that sputter coaters do not like being ignored for long
periods. Probably, the specimen chamber and vac lines adsorb moisture
and other gases from the laboratory and it takes the rotary pump
considerably longer to pump down (many hours versus 15-20 minutes).

You should pump the system down at least weekly for at least an hour.
After pumping for about 30 minutes, allow the Argon gas to leak
through the system. This really purges the residual gases. Then, we
find that if you fill the system with Argon, rather than letting it
fill with room air, it will take considerably less time to get into a
usable range next time.

John B.

####################################################################
John J. Bozzola, Ph.D., Director
Micro-Imaging and Analysis Center
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
####################################################################



From daemon Tue May 09 12:09:49 2000



From: MICHAEL DELANNOY :      delannoy-at-mail.jhmi.edu
Date: Tue, 9 May 2000 13:04:28 -0400 (EDT)
Subject: Bio-Rad 600

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hello all,
We are offering a Bio-Rad MRC 600 confocal microscopy system for
sale. Included are: Krypton-Argon Laser (low hours) Single, Double,Triple
label capability (lines available are 488,568,633 and 514nm) on a Nikon
Optiphot Microscope (10, 20, and 60 X)-included. Accompanying computer
system running COMOS ver 6.03 and SOM 4.56d with two color monitors and
a dye sublimation printer. Focus motor Laser stand and microscope vibration
platform are also included. All interested please call (410) 955-1365 or
write back. Thank You.

Mike Delannoy
JHMI Microscopy Facility



From daemon Tue May 09 13:56:51 2000



From: Paul Webster :      pwebster-at-mailhouse.hei.org
Date: 09 May 00 11:08:55 -0700
Subject: Spurr resin problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

------------------------------------------------------------------------
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Dear experts,

I know I should be kept out of the lab but I had to do some special resin embedding.
The problem is that I didn't read my own instructions and made up Spurr resin by adding all the ingredients together and then mixing. This means I added the DMAE before mixing the other components and have ended up with brittle blocks of inconsistant hardness.

I never thought I would be asking this but is there any way that I can recover these blocks to allow me to examine what I have embedded?
Paul Webster, Ph.D
House Ear Institute
2100 West Third Street
Los Angeles, CA 90057
phone:213 273 8026
fax: 213 413 6739
e-mail: pwebster-at-hei.org
http://www.hei.org/htm/aemi.htm



From daemon Tue May 09 23:57:19 2000



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Tue, 9 May 2000 14:46:13 -1000 (HST)
Subject: Film dessicator

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X-Authentication-Warning: halia.pbrc.hawaii.edu: tina owned process doing -bs


Hi, all you experts...

In spite of the digital camera on our LEO 912 EFTEM, we have a couple of
users who are going through huge quantities of film. I need to set up an
evacuated film dessicator (separate from the one on our older TEM), but I
find the non-glass, non-clear-plastic vacuum dessicators in the catalogs
at hand to be enormously expensive. Does anyone have a favorite vendor
and model, or a kludge? I remember one at Berkeley that I think was made
out of a pressure cooker hooked to a vacuum pump...

Mahalo!
Tina

80 degrees F, sunny blue skies, everything in bloom, and promise of surf.

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************



From daemon Tue May 09 23:57:21 2000



From: Ben Craft :      bcraft-at-uci.edu
Date: Tue, 9 May 2000 18:10:54 -0700 (PDT)
Subject: Sputter Coater

Contents Retrieved from Microscopy Listserver Archives
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I'm looking for a working used sputter coater for a descent price or
donation for a university lab. Please contact me at bcraft-at-uci.edu if you
have one available.

Thank you,


#######
#####\_O -Ben Craft-
####/\/}
#### /"
### \





From daemon Tue May 09 23:57:22 2000



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Wed, 10 May 2000 13:55:28 GMT+1200
Subject: Viruses and attachments

Contents Retrieved from Microscopy Listserver Archives
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While everyone is focussed on the ease with which viruses can be
transmitted as and within attachments, maybe it's a good time to ask
that postings to the list be only as text messages, not as
attachments.

cheers

rtch


Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand



From daemon Tue May 09 23:57:31 2000



From: jim :      jim-at-proscitech.com.au
Date: Wed, 10 May 2000 10:46:41 +1000
Subject: RE: Spurr resin problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Paul, I am not writing because of your "Dear experts" address. An expert is a
squirt under pressure, maybe that suited better the person who mis-mixed the
Spurr's.
Long time ago I read a note that brittle blocks sectioned better after soaking
them overnight in ethanol. I've never tried that, but there is a possibility.
Please let us know if that method is any good.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Wednesday, May 10, 2000 4:09 AM, Paul Webster
[SMTP:pwebster-at-mailhouse.hei.org] wrote:
}
}
} Dear experts,
}
} I know I should be kept out of the lab but I had to do some special resin
} embedding.
} The problem is that I didn't read my own instructions and made up Spurr resin
} by adding all the ingredients together and then mixing. This means I added
} the DMAE before mixing the other components and have ended up with brittle
} blocks of inconsistant hardness.
}
} I never thought I would be asking this but is there any way that I can
recover
} these blocks to allow me to examine what I have embedded?
} Paul Webster, Ph.D
} House Ear Institute
} 2100 West Third Street
} Los Angeles, CA 90057
} phone:213 273 8026
} fax: 213 413 6739
} e-mail: pwebster-at-hei.org
} http://www.hei.org/htm/aemi.htm
}



From daemon Sat May 13 11:48:10 2000



From: =?iso-8859-2?B?TOFi4XIgSuFub3M=?= :      labar-at-mfa.kfki.hu
Date: Fri, 12 May 2000 17:11:12 +0200
Subject: Need energy-filtered SAED ring-patterns

Contents Retrieved from Microscopy Listserver Archives
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Margaret,
Your message struck a chord here, and I would like to extend my
heartfelt sympathy. We've been there too. I have reached the point
where all the suppliers I contact have a 15-year history of preparing
quotations and tenders almost completely in vain. I don't know why
the sales reps. (or I) put up with it. That they do is a triumph of
hope over realistic expectation, and therefore a credit to all of them.
I think there should be a club for stressed out managers of poorly-
funded EM facilities. I would be one of the first to join. perhaps
some day we should organise a world congress if we can find a
venue large enough, thoough whether it would be advisable to have
a trade exhibition is open to question.
Best wishes
Chris

Date sent: Thu, 11 May 2000 14:43:05 -0400
To: Microscopy-at-sparc5.microscopy.com
} From: Margaret Dienelt {brannign-at-asrr.arsusda.gov}


Dear Colleagues,

I want to test a new computer program of mine that processes electron
diffraction ring-patterns from polycrystalline samples. I did test it with
patterns recorded on film. However, I would also like to test it on energy
filtered patterns that were recorded with a CCD (or imaging plate).

Could anyone of you send me such patterns from a single phase material with
random orientation? If you also characterized the same sample (especially if
you proved that the sample is not textured) and you published the results, I
could reference this publication of yours.

Thank you in advance.

Dr. Janos L. Labar
Research Institute for Technical Physics and Materials Science
H-1121 Budapest, Konkoly-Thege u. 29-33
Postal address: H-1525 Budapest-114, Po Box 49
Tel: (36)(1) 392-26-92
Fax: (36)(1) 275-49-96
Fax/phone: (36)(1) 395-92-32
home page: www.mfa.kfki.hu/~labar




From daemon Sat May 13 11:48:13 2000



From: Mark Riggs :      riggsm-at-svg.com
Date: Fri, 12 May 2000 15:01:23 -0400
Subject: cleaving tools small samples

Contents Retrieved from Microscopy Listserver Archives
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------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Your concerns and frustrations expressed are a recurrent thread on this site. It would be nice to be able to sit down with other facility managers and discuss common problems and possible solutions. I think our common concerns are such that they effect both materials and biological facilities. I am thinking of things such as: use guidelines, multi-user vs. service functions, formal courses and informal instruction for new users, equipment maintenance costs, justification of new equipment needs to administrators, advisor committee formats, funding sources, etc.
Perhaps we could arrange such an informal session at the upcoming M&M meeting. If you would be interested in such a session, take a look at the meeting schedule and suggest a time. Then I will ask the organizing committee to designate a room.
Debby

Debby Sherman, Manager Phone: 765-494-6666
Microscopy Center in Agriculture FAX: 765-494-5896
Dept. of Botany & Plant Pathology E-mail: sherman-at-btny.purdue.edu
Purdue University
1057 Whistler Building
West Lafayette, IN 47907-1057

--------------------------------------


Margaret,
Your message struck a chord here, and I would like to extend my
heartfelt sympathy. We've been there too. I have reached the point
where all the suppliers I contact have a 15-year history of preparing
quotations and tenders almost completely in vain. I don't know why
the sales reps. (or I) put up with it. That they do is a triumph of
hope over realistic expectation, and therefore a credit to all of them.
I think there should be a club for stressed out managers of poorly-
funded EM facilities. I would be one of the first to join. perhaps
some day we should organise a world congress if we can find a
venue large enough, thoough whether it would be advisable to have
a trade exhibition is open to question.
Best wishes
Chris

Date sent: Thu, 11 May 2000 14:43:05 -0400
To: Microscopy-at-sparc5.microscopy.com
} From: Margaret Dienelt {brannign-at-asrr.arsusda.gov}


recall reading of ibm's hunt for a silicon wafer cleaving tool capable of handling samples 5mm in diameter. any luck out there?

mark riggs
svg lithography
wilton, ct 06897
riggsm-at-svg.com



From daemon Sat May 13 11:48:15 2000



From: Douglas Keene :      DRK-at-shcc.org
Date: Fri, 12 May 2000 13:00:33 -0700 (Pacific Daylight Time)
Subject: Uranyl Formate

Contents Retrieved from Microscopy Listserver Archives
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We are contrasting connective tissue filaments by
negative stain technique using uranyl formate. Our
protocol is to adsorb filaments onto carbon film coated
grids, then to wash in two drops of water and two drops of
uranyl formate, removing each drop using filter paper but
not allowing the grid to dry until after the second drop of
UF. We can not charge the grid surface prior to specimen
adsorption or our specimens will not adsorb.

My question is to do with making uranyl formate. Our
formula is to boil 5ml water, add .0375 g uranyl formate
(our solid is very old...), stir for 20 minutes, then add
10 microlitter 5 M NaOH, stir for 20 minutes, then filter
through a 0.1 micron before use. We are not getting a
consistant staining pattern. Our wish is to see a uniform
coating of stain and we seldom see even single grid squares
evenly coated. We've tried different concentrations of
stain and also different volumes of NaOH. Any suggestions?
Does anyone know the purpose of boiling the water?

Thanks in advance,

Doug
----------------------
Douglas R. Keene
Associate Investigator
Shriners Hospital Research Facilities
3101 S.W. Sam Jackson Park Road
Portland, Oregon 97201
phone: 503-221-3434
FAX: 503-412-6894 (9-5 PST)
e-mail: DRK-at-shcc.org




From daemon Sat May 13 11:48:15 2000



From: Michael Bode :      mb-at-Soft-Imaging.com
Date: Fri, 12 May 2000 13:46:01 -0600
Subject: TEM: new developments in imaging systems?

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Margaret,

As a former user and current vendor of such systems as you are inquiring
about I can try to provide a bit of information regarding camera
improvements:

There have been a number of improvements, but I am not sure what you are
comparing the latest cameras against. Cameras are now usually cooled and
provide 12 bits per pixel, the number of pixels has gone up a bit (but
not much in general), and cameras read out faster than they used to (up
to 20 fps and more). I think all cameras now use a line transfer
mechanism, which makes shutters obsolete.
On the software side, real-time FFT and real-time shading correction can
be done now due to faster computers without special processing boards,
and there have been other software developments that make using the
cameras and computers easier.
Other changes that affect the usability of cameras is the use of
pneumatics to insert and retract the phosphors, higher frame rates for
live viewing with the camera, etc.

If you have questions, please give me a call, drop me an email, or go to
our web site.

Michael


Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Margaret Dienelt [mailto:brannign-at-asrr.arsusda.gov]
Sent: Thursday, May 11, 2000 12:43 PM
To: Microscopy-at-sparc5.microscopy.com


Hi,

Year after year I hopefully gather information about digital imaging
systems for TEMs (ours is a JEOL 100CX) , only to learn we have no
money. This year it looks like it might really happen but I have not
kept up with innovations in the field and am wondering the following:

1. Anything new in the last two years -- especially in terms of
cameras? I'm most familiar with the Gatan and AMT systems but their
web sites don't reflect much in the way of changes over a year ago.
2. With more and more microscopists finally getting their systems --
I'd love to get feedback.

Thanks,
Margaret

P.S. Would welcome contacts from vendors.

--
Margaret Dienelt

Plant Pathologist
Electron Microscopy Lab

Floral and Nursery Plants Research Unit
U.S. National Arboretum/Agricultural Research Service/USDA

B. 010A, Rm. 238, BARC-W
10300 Baltimore Avenue
Beltsville MD. 20705 USA

(301) 504-6097
Fax: (301) 504-5096



From daemon Sat May 13 11:48:19 2000



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Fri, 12 May 2000 16:29:56 -0700
Subject: Re: Spurr resin problem

Contents Retrieved from Microscopy Listserver Archives
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Patrick Echlin from Cambridge UK noted in private message that "strong"
agent who may dissolve epoxy is sodium methoxide. Thanks, Patrick.

Sergey.


} Date: Thu, 11 May 2000 16:06:39 -0700
} From: Sergey Ryazantsev {sryazant-at-ucla.edu}
} Subject: Re: Spurr resin problem
} X-Sender: sryazant-at-pop.ben2.ucla.edu
} To: Microscopy-at-sparc5.microscopy.com
} X-Mailer: QUALCOMM Windows Eudora Light Version 3.0.6 (32)
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu
http://www.bol.ucla.edu/~sryazant




From daemon Sat May 13 11:48:20 2000



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Fri, 12 May 2000 17:59:03 -0700
Subject: RE: Uranyl Formate

Contents Retrieved from Microscopy Listserver Archives
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Dear Doug,

My procedure for uranyl formate is a little bit simpler than yours:
0.05-0.1 g uranyl formate (0.5-1% final) + 10 ml deinozed (cell culture
quality or double distilled in the glass) water in the 15 ml plastic tube.
It dissolved at the same speed (even better) as acetate salt (UA). Usually
I am using rotator to shake slowly the tube with stain. It takes 0.5-1
hour to dissolve salt completely. I do not filter solution yet. The
difference between formate and acetate salts of the uranium is that formate
is light sensitive (UA - too, but less, less sensitive). You have to avoid
direct high intensity light. Usually I wrapped tube in alumina foil and
prepared the samples under diffused light moderate intensity (general
illumination in the lab, no local lights). Staining procedure is exact the
same as for acetate salt. The advantage of formate salt - it generates
smaller granularity (and less contrast than UA), spreaded sometime better
than acetate salt, and pH is higher. The disadvantage of the formate is
that the water solution is not stable: I do prepare fresh solution every
time I have to work with it. This is great disadvantage of the uranyl
formate. I guess, you may store solution in the dark at +4oC for couple of
days, but this is your own risk to experiment with that. As for staining
procedure, I would avoid any washes with just water. As a biochemist I am
under impression that ionic conditions is important to preserve "native"
structure of the sample. Therefore I am using the same buffer as for
sample to wash (usually I do not wash at all). Of coarse for any uranium
salts you have to avoid any phosphates in the buffer. Any Tris, MES, HEPES
buffers may be the good point to start. I don't know exactly how it works,
but it seems to me, that buffer in the wash may help spread satin better
(don't ask me why, I have no idea). If you have problem to dissolve uranyl
formate, you probably have to replace it on the fresh one (it is cheap).
Double-carbon technique may also help (you may call off line for details).
Good luck and sorry for the long message.

Sergey


} Date: Fri, 12 May 2000 13:00:33 -0700 (Pacific Daylight Time)
} From: Douglas Keene {DRK-at-shcc.org}
} Subject: Uranyl Formate
} Sender: drk-at-shcc.org
} To: microscopy-listserver {Microscopy-at-sparc5.microscopy.com}
} Reply-to: DRK-at-shcc.org
} X-Mailer: Simeon for Win32 Version 4.1.3 Build (39)
} Priority: NORMAL
} X-Authentication: none
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu
http://www.bol.ucla.edu/~sryazant




From daemon Sat May 13 11:48:25 2000



From: Caroline Schooley :      schooley-at-mcn.org
Date: Fri, 12 May 2000 20:44:02 -0700
Subject: RE: facility problems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} From Debby Sherman:
}
} Your concerns and frustrations expressed are a recurrent thread on this
} site. It would be nice to be able to sit down with other facility managers
} and discuss common problems and possible solutions. I think our common
} concerns are such that they effect both materials and biological
} facilities. I am thinking of things such as: use guidelines, multi-user
} vs. service functions, formal courses and informal instruction for new
} users, equipment maintenance costs, justification of new equipment needs
} to administrators, advisor committee formats, funding sources, etc.
} Perhaps we could arrange such an informal session at the upcoming M&M
} meeting. If you would be interested in such a session, take a look at the
} meeting schedule and suggest a time. Then I will ask the organizing
} committee to designate a room.

} Debby -

It's too late to add to the program now, but I attended a Long Beach 2001
LAC meeting last week, and there's a committee member there who wants to
organize something. It's almost too late to add programming even for that
one! The solution that I suggested is to start an annual breakfast or
lunch for facility managers, taking care to avoid other large scheduled
meetings (which aren't listed in the program summary). It might even be
possible to get more time Sunday afternoon, pre-opening reception. Contact
the LAC chairs for that: Stacie Kirsch for Philly & Zed Mason for Long
Beach.

Caroline Schooley


Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/PCI/pci.html




From daemon Mon May 15 08:19:03 2000



From: Mark Aindow :      maindow-at-ims.uconn.edu
Date: Mon, 15 May 2000 08:28:37 -0400
Subject: Postdoctoral Position

Contents Retrieved from Microscopy Listserver Archives
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University of Connecticut
Institute for Materials Science

Postdoctoral Research Position in Electron Microscopy Studies
of Fatigue Crack Initiation Sites in Ti-6-4 Alloys

The Institute for Materials Science (IMS) at UConn is an
interdisciplinary center with the threefold mission of fostering
education, research and outreach in all areas of the materials
sciences. Applications are invited for a Postdoctoral Position
to study the microcrystallography of fatigue crack initiation
sites in Ti 6-4 alloys. The appointee will be involved in electron
microscopy studies of failed test pieces produced at Pratt and
Whitney in the previous phase of this program. It is envisaged
that this work will involve extensive SEM and TEM studies in
the IMS at UConn with some use of the FIB/TEM/STEM and
OIM facilities in the High Temperature Materials Laboratory at
Oak Ridge National Laboratory. Candidates should hold a PhD
in Materials Science, Physics or a related discipline and must
have extensive hands-on experience in a broad range of electron
microscopy techniques. Experience in the assessment of deformation
substructures would also be beneficial. The appointment is for one
year in the first instance and is available from June 1st. Screening
of the applications will begin immediately and will continue until the
post is filled.

Applications from under-represented groups, including minorities,
women and people with disabilities are encouraged.

Interested candidates should send a curriculum vitae, including
publication list, and the names of at least three referees with
postal addresses, telephone numbers and Email addresses to:
Dr. M. Aindow, Institute for Materials Science, University of
Connecticut, 97 North Eagleville Road, U-3136, Storrs,
CT 06269-3136 USA. Email: maindow-at-ims.uconn.edu
--
*********************************************************

Mark Aindow, Associate Professor,
Department of Metallurgy and Materials Engineering
Institute of Materials Science, U-136
University of Connecticut, Storrs,
CT 06269-3136, USA

Tel: +1 (860) 486-2644
FAX: +1 (860) 486-4745
Email: maindow-at-ims.uconn.edu

**********************************************************



From daemon Mon May 15 08:19:04 2000



From: Suzannah Mayo :      smayo-at-southernmicro.com
Date: Mon, 15 May 2000 09:02:30 -0400
Subject: Particle size analysis, flyash preparation

Contents Retrieved from Microscopy Listserver Archives
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Hello All,

I have a question concerning sample preparation for brightfield and
polarized particle size analysis. My customer is trying to devise an
experiment to analyze fly ash collected on a filter during smokestack
emissions testing. He collects ~1gr. per sample, however, more is
easily possible. The sample is clumped and incongruent and he would
like a simple protocol for proper sample dispersion on a slide.

Thank you,
Suzannah Mayo



From daemon Mon May 15 17:35:35 2000



From: HDMHOS-at-aol.com
Date: Mon, 15 May 2000 08:19:25 -0500
Subject: pp wires

Contents Retrieved from Microscopy Listserver Archives
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Hello there everyone,
I am having hard time cutting gold coated polypropylene (pp) wires. I am
using a diamond knife, I have embedded the samples into epoxy and hardener, I
will be doing TEM. But when I cut, I get good thickness but the slice keep
rolling up. I have been trying for the past three days and do not have any
luck.
I have change the ratio of the epoxy to hardener from10/4 to 10/6 still no
luck.
Can anyone suggest some changes. Is cryogenic an option here.

Thank you all,

Briget Ngampa
28 Cedar street
LOWELL,MA 01852
Tel (978) 970 0433
Fax (978) 937 2297
EMAIL: hdmhos-at-aol.com




From daemon Mon May 15 17:35:37 2000



From: Barbara Foster :      mme-at-map.com
Date: Mon, 15 May 2000 11:02:36 -0400
Subject: Re: cleaving tools small samples

Contents Retrieved from Microscopy Listserver Archives
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Hi,
You may want to check SELA. They have an evergrowing line of cleavers for
the semiconductor industry. Contact Efrat Raz: efrat-at-sela.com

Caveat: MME has no financial interest in this product

Good hunting
Barbara Foster,President
Microscopy/Microscopy Education
125 Paridon Street Suite 102
Springfield, MA 01118-2130
PH: 413-746-6931 FX: 413-746-9311 email: MME-at-map.com
Website: www.MME-Microscopy.com/education

America's first national consortium of microscopy specialists offering
customized on-site training in all areas of microscopy, image analysis, and
sample preparation
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^


At 03:01 PM 5/12/00 -0400, Mark Riggs wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Mon May 15 17:35:39 2000



From: HILDEGARD CROWLEY :      hcrowley-at-du.edu
Date: Mon, 15 May 2000 10:34:11 -0600 (MDT)
Subject: Re: Spurr resin problem

Contents Retrieved from Microscopy Listserver Archives
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On Thu, 11 May 2000, Sergey Ryazantsev wrote:

} ------------------------------------------------------------------------
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} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
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} -----------------------------------------------------------------------.
}
}
} Hildy hello,
}
} I could not understand, how polymerized (mean crosslinked) epoxy may be
} dissolved back in PO? You have to break chemical bonds between polymer's
} chains first and than it will become soluble. I believe, there are some
} very strong oxidizing agents should be used in order to break chemical
} bonds in the epoxies.
}
} Sergey.
}
}
}
} } Date: Thu, 11 May 2000 11:28:43 -0600 (MDT)
} } From: HILDEGARD CROWLEY {hcrowley-at-du.edu}
} } Subject: Re: Spurr resin problem
} } X-Sender: hcrowley-at-odin.cair.du.edu
} } To: Paul Webster {pwebster-at-mailhouse.hei.org}
} } Cc: MSA listserver submission {Microscopy-at-sparc5.microscopy.com}
} }
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} }
} }
} } On Tue, 9 May 2000, Paul Webster wrote:
} }
} } } ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } -----------------------------------------------------------------------.
} } }
} } }
} } } Dear experts,
} } }
} } } I know I should be kept out of the lab but I had to do some special
} resin embedding.
} } } The problem is that I didn't read my own instructions and made up Spurr
} resin by adding all the ingredients together and then mixing. This means I
} added the DMAE before mixing the other components and have ended up with
} brittle blocks of inconsistant hardness.
} } }
} } } I never thought I would be asking this but is there any way that I can
} recover these blocks to allow me to examine what I have embedded?
} } } Paul Webster, Ph.D
} } } House Ear Institute
} } } 2100 West Third Street
} } } Los Angeles, CA 90057
} } } phone:213 273 8026
} } } fax: 213 413 6739
} } } e-mail: pwebster-at-hei.org
} } } http://www.hei.org/htm/aemi.htm
} } }
} } }
} } }
} } Hi,
} }
} } First try this: Get a beaker of water, heat it to the highest temperature
} } at which your blocks were polymerized, then subtract 5 deg. After temp
} } has been reached, put in one block. Leave it for 15 min. Take it out,
} } trim it, and section it immediately. Too brittle? Repeat the water soak
} } for 15 min. Repeat again. Not much can be accomplished after one hour of
} } soaking, but this may be different in your case. Sometimes in desperation
} } when I wanted 4 micrometer thick sections from difficult material I have
} } soaked blocks overnight with good success.
} }
} } One time I was given immensely valuable blocks which were so bad (kind
} } expression) that they curled my hair. They could not be cut. I had to
} } get the epoxy out and reembed. (The micrographs later ended up in a
} } publication in the Comp. Neurol. Journal)
} }
} } What I did was to rotate the blocks in vials continuously in the REVERSE
} } order in which they were embedded, with much elongated times. That is,
} } first they went into PO and epoxy (same formulation as the bad embedment)
} } 3:1, for hours, the 2:1 for the rest of the day, then 1:1 for overnight,
} } then pure PO for a whole day. All the above were done with numerous
} } changes. Once I was in PO, pure for a day, most of the epoxy had left the
} } tissue. I then remembedded the same way I deembedded. What a pain. The
} } blocks were never wonderful, but they sectioned OK and went for
} } publication. It should work for Spurr's. Don't give up. When
} } deembedding, I left out the accelerator, of course. It helps to have some
} } very good chocolate on hand for this maneuver.
} }
} } Good luck,
} } Hildy
} }
} } Hildegard H. Crowley
} } University of Denver
} }
} }
} }
} _____________________________________
}
} Sergey Ryazantsev Ph. D.
} Electron Microscopy
} UCLA School of Medicine
} Department of Biological Chemistry
} Box 951737
} Los Angeles, CA 90095-1737
}
} Phone: (310) 825-1144
} Pager: (310) 845-0248
} FAX (departmental): (310) 206-5272
} mailto:sryazant-at-ucla.edu
} http://www.bol.ucla.edu/~sryazant
}
}
}
}
Hi,

Yes, it is a mystery to me that one can get old epoxy out of tissue with
PO. However, I have done it at least 3 times with enough success to
collect data. The only thing I can think of is that at least 10% of
monomers never bind due to the low embed temps we use for our TEM work.
Those will surely dissolve out leaving holes. (I have seen this). PO is
the simplest of epoxies and a very strong solvent. That is all I know. I
never do the "REVERSE" embed unless forced to do it, because it is so
difficult dealing with the final cutting and the staining. And then the
"new" sections are unstable and uneven. I would rather clean a bathroom!
Bye,
Hildy



From daemon Mon May 15 17:35:39 2000



From: HILDEGARD CROWLEY :      hcrowley-at-du.edu
Date: Mon, 15 May 2000 10:38:54 -0600 (MDT)
Subject: Re: Spurr resin problem

Contents Retrieved from Microscopy Listserver Archives
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On Fri, 12 May 2000, Sergey Ryazantsev wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Patrick Echlin from Cambridge UK noted in private message that "strong"
} agent who may dissolve epoxy is sodium methoxide. Thanks, Patrick.
}
} Sergey.
}
}
} } Date: Thu, 11 May 2000 16:06:39 -0700
} } From: Sergey Ryazantsev {sryazant-at-ucla.edu}
} } Subject: Re: Spurr resin problem
} } X-Sender: sryazant-at-pop.ben2.ucla.edu
} } To: Microscopy-at-sparc5.microscopy.com
} } X-Mailer: QUALCOMM Windows Eudora Light Version 3.0.6 (32)
} }
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Hildy hello,
} }
} } I could not understand, how polymerized (mean crosslinked) epoxy may be
} } dissolved back in PO? You have to break chemical bonds between polymer's
} } chains first and than it will become soluble. I believe, there are some
} } very strong oxidizing agents should be used in order to break chemical
} } bonds in the epoxies.
} }
} } Sergey.
} }
} }
} }
} } } Date: Thu, 11 May 2000 11:28:43 -0600 (MDT)
} } } From: HILDEGARD CROWLEY {hcrowley-at-du.edu}
} } } Subject: Re: Spurr resin problem
} } } X-Sender: hcrowley-at-odin.cair.du.edu
} } } To: Paul Webster {pwebster-at-mailhouse.hei.org}
} } } Cc: MSA listserver submission {Microscopy-at-sparc5.microscopy.com}
} } }
} } } ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } -----------------------------------------------------------------------.
} } }
} } }
} } }
} } }
} } } On Tue, 9 May 2000, Paul Webster wrote:
} } }
} } } } ------------------------------------------------------------------------
} } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } } -----------------------------------------------------------------------.
} } } }
} } } }
} } } } Dear experts,
} } } }
} } } } I know I should be kept out of the lab but I had to do some special
} } resin embedding.
} } } } The problem is that I didn't read my own instructions and made up Spurr
} } resin by adding all the ingredients together and then mixing. This means I
} } added the DMAE before mixing the other components and have ended up with
} } brittle blocks of inconsistant hardness.
} } } }
} } } } I never thought I would be asking this but is there any way that I can
} } recover these blocks to allow me to examine what I have embedded?
} } } } Paul Webster, Ph.D
} } } } House Ear Institute
} } } } 2100 West Third Street
} } } } Los Angeles, CA 90057
} } } } phone:213 273 8026
} } } } fax: 213 413 6739
} } } } e-mail: pwebster-at-hei.org
} } } } http://www.hei.org/htm/aemi.htm
} } } }
} } } }
} } } }
} } } Hi,
} } }
} } } First try this: Get a beaker of water, heat it to the highest temperature
} } } at which your blocks were polymerized, then subtract 5 deg. After temp
} } } has been reached, put in one block. Leave it for 15 min. Take it out,
} } } trim it, and section it immediately. Too brittle? Repeat the water soak
} } } for 15 min. Repeat again. Not much can be accomplished after one hour of
} } } soaking, but this may be different in your case. Sometimes in desperation
} } } when I wanted 4 micrometer thick sections from difficult material I have
} } } soaked blocks overnight with good success.
} } }
} } } One time I was given immensely valuable blocks which were so bad (kind
} } } expression) that they curled my hair. They could not be cut. I had to
} } } get the epoxy out and reembed. (The micrographs later ended up in a
} } } publication in the Comp. Neurol. Journal)
} } }
} } } What I did was to rotate the blocks in vials continuously in the REVERSE
} } } order in which they were embedded, with much elongated times. That is,
} } } first they went into PO and epoxy (same formulation as the bad embedment)
} } } 3:1, for hours, the 2:1 for the rest of the day, then 1:1 for overnight,
} } } then pure PO for a whole day. All the above were done with numerous
} } } changes. Once I was in PO, pure for a day, most of the epoxy had left the
} } } tissue. I then remembedded the same way I deembedded. What a pain. The
} } } blocks were never wonderful, but they sectioned OK and went for
} } } publication. It should work for Spurr's. Don't give up. When
} } } deembedding, I left out the accelerator, of course. It helps to have some
} } } very good chocolate on hand for this maneuver.
} } }
} } } Good luck,
} } } Hildy
} } }
} } } Hildegard H. Crowley
} } } University of Denver
} } }
} } }
} } }
} } _____________________________________
} }
} } Sergey Ryazantsev Ph. D.
} } Electron Microscopy
} } UCLA School of Medicine
} } Department of Biological Chemistry
} } Box 951737
} } Los Angeles, CA 90095-1737
} }
} } Phone: (310) 825-1144
} } Pager: (310) 845-0248
} } FAX (departmental): (310) 206-5272
} } mailto:sryazant-at-ucla.edu
} } http://www.bol.ucla.edu/~sryazant
} }
} }
} }
} }
} _____________________________________
}
} Sergey Ryazantsev Ph. D.
} Electron Microscopy
} UCLA School of Medicine
} Department of Biological Chemistry
} Box 951737
} Los Angeles, CA 90095-1737
}
} Phone: (310) 825-1144
} Pager: (310) 845-0248
} FAX (departmental): (310) 206-5272
} mailto:sryazant-at-ucla.edu
} http://www.bol.ucla.edu/~sryazant
}
}
}
}
Hi,

Absolutely sodium methoxide will take the epoxy out of the tissue.
However, in our laboratory it caused so much tissue damage (since epoxies
actually bind with proteins in the tissue and not simply throw a net
through and around the tissue like the acrylics) that after reembedding it
was not useful for collecting data.
Somebody else might have better results than myself with that method, so
we should not discard the idea.

Bye,
Hildy



From daemon Mon May 15 17:35:39 2000



From: Stephen McCartney :      stmccart-at-vt.edu
Date: Mon, 15 May 2000 13:51:14 -0400
Subject: pp wires

Contents Retrieved from Microscopy Listserver Archives
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Briget: Polypropylene has a Tg of about -19C. Whenever I microtome I
always cut at least 15-20 degrees below the Tg. If you are trying to cut
these samples at room temperature my guess is this is your problem. If you
need to embed you can still do this when cutting at cryo temperatures by
trimming as much of the epoxy away as possible. If you leave a very thin
layer of epoxy around your sample you should still get good sections even
though you will get some chatter in the epoxy region. Steve



Hello there everyone,
I am having hard time cutting gold coated polypropylene (pp) wires. I am
using a diamond knife, I have embedded the samples into epoxy and hardener, I
will be doing TEM. But when I cut, I get good thickness but the slice keep
rolling up. I have been trying for the past three days and do not have any
luck.
I have change the ratio of the epoxy to hardener from10/4 to 10/6 still no
luck.
Can anyone suggest some changes. Is cryogenic an option here.

Thank you all,

Briget Ngampa
28 Cedar street
LOWELL,MA 01852
Tel (978) 970 0433
Fax (978) 937 2297
EMAIL: hdmhos-at-aol.com


Stephen McCartney
Research Associate
2108 Hahn Hall
Materials Institute
Virginia Tech
Blacksburg, VA 24061-0344
USA

TEL: 540-231-9765
FAX: 540-231-8517



From daemon Mon May 15 17:35:40 2000



From: jmkrupp-at-cats.ucsc.edu (Jon Krupp)
Date: Mon, 15 May 2000 11:19:12 -0700
Subject: TEM help selecting prep. equip.

Contents Retrieved from Microscopy Listserver Archives
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Dear List:

A newly appointed researcher here has asked my advice on several pieces of
TEM spec. prep equipment. I turn to you for helpful suggestions.

The research involves serial sectioning biological tissues and many grids.
EM is a minor, but essential component of the project. The lab runs through
dozens of grids and hundreds of pictures, but only 4 - 6 times a year. Big
bursts of activity followed by long periods of analysis and investigations
using other techniques. Of the hundreds of pictures taken, they may only
use a few for data.

The researcher is looking for ideas on what choices are available, how
useful, and approximate costs of the following:

Digital imaging to add to our existing JEOL 1200EX TEM - I have seen other
threads on this topic and would welcome any new ideas. Digital imaging will
save a lot of time and money since they discard so many pictuers, but the
question of image quality is one I am to investigate.

Ultra microtome - We have an older A/O Ultracut (the model before it became
the Reichert Ultracut E) which is OK for the sectioning we do in the
general lab. But she wants a new one for her exclusive use. The question is
whether a new microtome will allow folks in her lab to do serial
sectioning any faster or easier, or by less skilled users, than our current
system.

Staining machine - Anyone have info on staining machines or systems for
lots of TEM grids. I have never had to do so many grids that this was an
issue, so I have never kept up on the offerings. If you know of something
and/or have experience let me know. Again, this is something she would keep
in her lab.

Tissue processing machine - Same as above for me, never did so much at one
time that I ever thought I needed one of these. The samples to be processed
are C. elegans, anything available that could do these unattended? How
about upkeep and volumes of chemicals needed. Also an item to be kept in
her lab.

I will filter and pass on your comments. Anything you might offer will, as
always, be received with appreciation and thanks.

Jonathan Krupp
Microscopy & Imaging Lab
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu




From daemon Mon May 15 17:35:41 2000



From: Greg Erdos :      gwe-at-biotech.ufl.edu
Date: Mon, 15 May 2000 16:07:58 -0400
Subject: Re: TEM help selecting prep. equip.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Jon,
I can speak to the digital camera at least. We have been using a
Gatan BioScan on our microscope and it serves our needs for 95% of the
images that we produce. It is a very good system, when it is working. We
have had more problems with it than I would have expected. We bought the
system early in the production and perhaps they have worked out the bugs by
now.
-------------------------------------.


} Dear List:
}
} A newly appointed researcher here has asked my advice on several pieces of
} TEM spec. prep equipment. I turn to you for helpful suggestions.
}
} The research involves serial sectioning biological tissues and many grids.
} EM is a minor, but essential component of the project. The lab runs through
} dozens of grids and hundreds of pictures, but only 4 - 6 times a year. Big
} bursts of activity followed by long periods of analysis and investigations
} using other techniques. Of the hundreds of pictures taken, they may only
} use a few for data.
}
} The researcher is looking for ideas on what choices are available, how
} useful, and approximate costs of the following:
}
} Digital imaging to add to our existing JEOL 1200EX TEM - I have seen other
} threads on this topic and would welcome any new ideas. Digital imaging will
} save a lot of time and money since they discard so many pictuers, but the
} question of image quality is one I am to investigate.
}
} Ultra microtome - We have an older A/O Ultracut (the model before it became
} the Reichert Ultracut E) which is OK for the sectioning we do in the
} general lab. But she wants a new one for her exclusive use. The question is
} whether a new microtome will allow folks in her lab to do serial
} sectioning any faster or easier, or by less skilled users, than our current
} system.
}
} Staining machine - Anyone have info on staining machines or systems for
} lots of TEM grids. I have never had to do so many grids that this was an
} issue, so I have never kept up on the offerings. If you know of something
} and/or have experience let me know. Again, this is something she would keep
} in her lab.
}
} Tissue processing machine - Same as above for me, never did so much at one
} time that I ever thought I needed one of these. The samples to be processed
} are C. elegans, anything available that could do these unattended? How
} about upkeep and volumes of chemicals needed. Also an item to be kept in
} her lab.
}
} I will filter and pass on your comments. Anything you might offer will, as
} always, be received with appreciation and thanks.
}
} Jonathan Krupp
} Microscopy & Imaging Lab
} University of California
} Santa Cruz, CA 95064
} (831) 459-2477
} jmkrupp-at-cats.ucsc.edu
}
}

Gregory W. Erdos, Ph.D.
Assistant Director, Biotechnology Program
PO Box 110580
University of Florida
Gainesville, FL 32611

Ph. 352-392-1295 Fax: 352-846-0251



From daemon Mon May 15 17:35:43 2000



From: Mark West :      mwest-at-ifcsun1.ifisiol.unam.mx
Date: Mon, 15 May 2000 17:29:02 -0600 (Hora est‡ndar de MŽxico)
Subject: starch granules

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all - a first attempt at unloading a problem onto the listserver!

I am trying to get thin sections of blue-green algae that are loaded with
starch granules. The problem is that every time we do the preps, we end up
with cells that lose some granules, leaving a gaping hole, or of starch
granules that have holes in the centre. I'm using a standard sort of
method - fix in phosphate + 2.5% glut 2 hours, osmium 1% 2 hours,
dehydrate in ethanol series, finishing with prop. ox, 3:1 prop. ox/resin
around 2 h, 1:1 2 or 3h then another change overnight, a couple of 100%
resin changes for 1/2 to one day, then polymerization 48h/60C. The fixing
and EtOH steps are done on ice, the rest at room temp, the resin
infiltration is done on a rotating mixer. We've tweaked the method around
but nothing seems to work. I've tried using LR White as well, but with no
luck. Can anybody out there help!?

Mark






********************************************
Mark West,
Unidad de Microscopia Electronica,
(Electron Microscopy Unit)
Instituto de Fisiologia Celular,
Universidad Nacional Autonoma de Mexico,
04510 Mexico D.F.

tel (unidad/lab) *(525) 622 5610*
(casa/home) (525) 619 3020
Fax (525) 616 2282
********************************************




From daemon Mon May 15 17:49:17 2000



From: Mark Armogida :      mark_armogida-at-tedpella.com
Date: Mon, 15 May 2000 17:39:09 -0500
Subject: TEM, Hitachi model Hu 11a

Contents Retrieved from Microscopy Listserver Archives
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"I am Mark Armogida, VP of Engineering at Ted Pella, Inc. in Redding,
California, USA. We have an old (1964) TEM, Hitachi model Hu 11a, that
is a decommissioned instrument which we are trying to give away for
removal/shipping costs. It was operational a year ago and kept under
vacuum since then. We have the instruction manual. This unit consists of
the main body with diffusion pumps, a large electronics console, a large
oil filled resistor and mechanical pumps. The oil was analyzed and does
not contain PCB's. I estimate that this system weighs several thousand
pounds. I will have it removed for salvage in a week, so please contact
me if you are interested in this system."


Mark Armogida
VP, Engineering & Production
Ted Pella, Inc.




From daemon Mon May 15 17:49:18 2000



From: Pamela Neill :      Pamela.Neill-at-alconlabs.com
Date: Mon, 15 May 2000 17:43:33 -0500
Subject: Philips 300 available

Contents Retrieved from Microscopy Listserver Archives
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I am sending this for a colleague. It was a wonderful little scope while I
was there.
} LifeCell Corporation (The Woodlands, TX and Branchburg, NJ) is closing
their facility in The Woodlands.
} LifeCell has available for donation a Philips EM300 TEM. The scope was in
} working order when shut down in December 1999.
} If you are interested please contact:
} Sy Griffey, Ph.D.
} 908-947-1143 or sgriffey-at-lifecell.com
}




From daemon Mon May 15 18:05:43 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 15 May 2000 16:02:58 -0700
Subject: Re: SEM: digital 4x5 backs

Contents Retrieved from Microscopy Listserver Archives
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At 01:38 PM 5/10/00, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

It seems to me that no digital camera system would work on a SEM
in place of a Polaroid or other film-based output device. Since the
recording CRT in a SEM is based on a sequential line scan, one
would need a camera that would capture each line as it is produced.
Most digital backs are single or triple pass units of a single linear
set of sensors. There are other cameras that do snapshot capture
but even these would not work since the whole image is not present
on the record CRT at the time of taking a picture with the digital
camera. The final image is generated sequentially, line by line,
on the record CRT.

If the SEM image is stored in a frame buffer, the buffer can be
converted to RS-170 TV video and frame grabbed. But the
best that this would typically do is 640 lines.

Its an interesting problem and dilemma about being in a situation
where digital camera products simply won't work in place of
film. But since the goal is to obtain a digital file, why not start
with a digital interface? For example, a passive digital capture
system would transfer the record CRT information to computer
and directly result in a nice digital file. Alternatively, for some
systems, an active system can be applied to directly control the
SEM's beam. In doing so, the range of final digital image
resolution is limited only by the attached hardware system.

gary g.


~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Modern surfers use PC boards. You can too at
http://photoweb.net
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~



From daemon Mon May 15 19:10:17 2000



From: Mark Armogida :      mark_armogida-at-tedpella.com
Date: Mon, 15 May 2000 17:02:06 -0700
Subject: TEM for givaway

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am Mark Armogida, VP of Engineering at Ted Pella, Inc. in Redding,
California, USA. We have an old (1964) TEM, Hitachi model Hu 11a, that
is a decommissioned instrument which we are trying to give away for
removal/shipping costs. It was operational a year ago and kept under
vacuum since then. We have the instruction manual. This unit consists of
the main body with diffusion pumps, a large electronics console, a large
oil filled resistor and mechanical pumps. The oil was analyzed and does
not contain PCB's. I estimate that this system weighs several thousand
pounds. I will have it removed for salvage in a week, so please contact
me if you are interested in this system. I can be reached by e-mail or
by phone at 530-241-2200 ext 212 between the hours of 8:00am and 5:00pm
pacific time zone.



From daemon Mon May 15 17:35:38 2000



From: Dean Miller :      miller-at-anl.gov
Date: Mon, 15 May 2000 11:21:23 -0500
Subject: microscopist/materials scientist position at ANL

Contents Retrieved from Microscopy Listserver Archives
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Electron Microscopist / Materials Scientist
Materials Science Division, Argonne National Laboratory

The Materials Science Division at Argonne National Laboratory invites
applications for a Staff Scientist position in Electron Microscopy. The
candidate should have a strong background in the various techniques of
electron microscopy and a very strong interest in application of these
techniques
in materials science. The candidate should have a state-of-the-art
knowledge of either analytical transmission electron microscopy including high
spatial resolution x-ray and electron spectroscopy or the application of
electron holography and Lorentz imaging, with a particular emphasis on
field emission (S)TEM. Candidates with exceptional experience in other areas
of microscopy will also be considered.

Areas of particular research interest include defects and interfaces in
materials, in situ studies of critical phenomena, irradiation and ion
implantation effects, and applications of electron holography and Lorentz
imaging. A familiarity with research in one or more of the following areas is
highly desirable: magnetic and superconducting materials, irradiation
effects, ferroelectrics, nanoscale materials, diamond films, and
non-crystalline materials. The successful candidate will work closely with
Electron
Microscopy Center personnel and other research groups within the Materials
Science Division to develop strong research efforts in one or more of these
areas.

Interested candidates should send a curriculum vitae, a brief statement
of research interests and plans, and the names and contact information of
three references to:
Susan Walker, Employment and Placement
Box MSD-210121
9700 S. Cass Avenue
Argonne, IL 60439
TDD: 630-252-7722

Questions can be addressed to Dr. Dean J. Miller, Materials Science
Division, Argonne National Laboratory, 9700 S. Cass Ave., Argonne, IL 60439,
tel. 630-252-4108 (with voice mail), fax 630-252-7529, or miller-at-anl.gov.

Argonne National Laboratory is a multidisciplinary center of energy
research and related scientific studies and is operated by the University of
Chicago for the U.S. Department of Energy. Argonne National Laboratory is a
federal contractor and complies with all federal contractor rules and
regulations regarding the maintenance and implementation of our Affirmative
Action Program.


---------------------------
Dean J. Miller
Materials Science Division
Argonne National Laboratory
Argonne, IL 60439

630-252-4108 (office)
630-252-7777 (FAX)

miller-at-anl.gov






From daemon Tue May 16 07:36:47 2000



From: Ken Converse :      qualityimages-at-netrax.net
Date: Tue, 16 May 2000 07:50:54 -0700
Subject: Re: SEM: digital 4x5 backs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dr. Gary Gaugler wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} At 01:38 PM 5/10/00, you wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} }
} } I'm curious ... has anyone tried simply installing a digital
} } 4x5 back in place of a Polaroid 4x5 back??? For example, see:
} }
} } http://www.phaseone.com/brochures/powerfx.html
} }
} } cheerios, shAf
}
} It seems to me that no digital camera system would work on a SEM
} in place of a Polaroid or other film-based output device. Since the
} recording CRT in a SEM is based on a sequential line scan, one
} would need a camera that would capture each line as it is produced.
} Most digital backs are single or triple pass units of a single linear
} set of sensors. There are other cameras that do snapshot capture
} but even these would not work since the whole image is not present
} on the record CRT at the time of taking a picture with the digital
} camera. The final image is generated sequentially, line by line,
} on the record CRT.
}
} If the SEM image is stored in a frame buffer, the buffer can be
} converted to RS-170 TV video and frame grabbed. But the
} best that this would typically do is 640 lines.
}
} Its an interesting problem and dilemma about being in a situation
} where digital camera products simply won't work in place of
} film. But since the goal is to obtain a digital file, why not start
} with a digital interface? For example, a passive digital capture
} system would transfer the record CRT information to computer
} and directly result in a nice digital file. Alternatively, for some
} systems, an active system can be applied to directly control the
} SEM's beam. In doing so, the range of final digital image
} resolution is limited only by the attached hardware system.
}
} gary g.
}
} ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
} Modern surfers use PC boards. You can too at
} http://photoweb.net
} ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

Shaf,
Aside from the problems already mentioned, the back alone (without
computer) is almost as expensive as the smallest SEMs. It also has
about 4 times the resolution of the best recording systems out there
(ETEC) so what you'd get is a lot of empty (information-wise) pixels.
It might be adaptable to TEM, though.

For an SEM an active digital control could be set up to gather 10K x 10K
images, but the stability of the column drivers becomes very important.
Some could handle it fairly well while some would again be useless
because their drivers have no noise immunity due to the fact that their
push-pull mag drivers were designed backwards and all powere supply
noise is passed directly to the scan coils.

Ken Converse
Quality Images
third party SEM service
Delta, PA



From daemon Tue May 16 07:46:49 2000



From: youmay4-at-aol.com ()
Date: Tue, 16 May 2000 07:40:06 -0500
Subject: nutritionist microscopist

Contents Retrieved from Microscopy Listserver Archives
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Email: youmay4-at-aol.com
Name: mil may

Question: where can I find certified nutritionist microscopist in different
parts of the us?

---------------------------------------------------------------------------




From daemon Tue May 16 07:46:49 2000



From: Jan Coetzee :      janc-at-ccnet.up.ac.za
Date: Tue, 16 May 2000 07:38:03 -0500
Subject: Starch granules in blue-green algae.

Contents Retrieved from Microscopy Listserver Archives
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Embedding and sectioning material containing a lot of starch is always a
problem. If you do not need to look at the structure of the mature
starch grains, you could try embedding the algae first thing in the
morning before they have had time to produce the starch. Most plants
have a low starch content after being kept in the dark for approx 12
hours.
For instance, active, growing leaves are difficult to section when
collected midday, but early morning collecting makes for easy
sectioning.


Jan Coetzee



Mark West wrote:

} I am trying to get thin sections of blue-green algae that are loaded with
} starch granules. The problem is that every time we do the preps, we end up
} with cells that lose some granules, leaving a gaping hole, or of starch
} granules that have holes in the centre. I'm using a standard sort of
} method - fix in phosphate + 2.5% glut 2 hours, osmium 1% 2 hours,
} dehydrate in ethanol series, finishing with prop. ox, 3:1 prop. ox/resin
} around 2 h, 1:1 2 or 3h then another change overnight, a couple of 100%
} resin changes for 1/2 to one day, then polymerization 48h/60C. The fixing
} and EtOH steps are done on ice, the rest at room temp, the resin
} infiltration is done on a rotating mixer. We've tweaked the method around
} but nothing seems to work. I've tried using LR White as well, but with no
} luck. Can anybody out there help!?
}


--
Prof Jan Coetzee
Lab for Microscopy and Microanalysis
University of Pretoria, South Africa.
Tel: 012-420-2075, Fax 012-362-5150
www.up.ac.za/science/electron/emunit1.htm




From daemon Tue May 16 07:46:49 2000



From: moshe_marc-at-gohip.com ()
Date: Tue, 16 May 2000 07:39:23 -0500
Subject: test the quality of difraction limited microscope

Contents Retrieved from Microscopy Listserver Archives
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Email: moshe_marc-at-gohip.com
Name: moshe marcovitch

Question: Dear sir
In order to test the quality of difraction limited microscope I am looking
for reticles that will enable me to produce difraction patterns of about
0.2 micron source can you advice where can I find such reticles . Who can
produce them for me if they are not available comercially .
Best regards
Moshe Marcovitch

---------------------------------------------------------------------------




From daemon Tue May 16 08:46:40 2000



From: tracy gales :      tl_gales-at-fccc.edu
Date: Tue, 16 May 2000 09:37:17 -0400
Subject: Starch granules

Contents Retrieved from Microscopy Listserver Archives
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Mark,

I've seen references to using potassium ferrocyanide reduced osmium
tetroxide to help preserve glycogen. Karnovsky(1971) Use of
ferrocyanide-reduced OsO4 in EM. In Proc.14th Annu.Meet. Am. Soc. Cell
Biol., p. 146. Abstract 284.

Tracy Gales
Fox Chase Cancer Center
Electron Microscopy Facility
7701 Burholme Ave.
Philadelphia PA 19111
Tel. 215-728-2484
Fax 215-728-2412




From daemon Tue May 16 09:16:56 2000



From: John Foust :      jfoust-at-threedee.com
Date: Tue, 16 May 2000 09:06:16 -0500
Subject: Re: SEM: digital 4x5 backs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 04:02 PM 5/15/00 -0700, Dr. Gary Gaugler wrote:
} It seems to me that no digital camera system would work on a SEM
} in place of a Polaroid or other film-based output device. Since the
} recording CRT in a SEM is based on a sequential line scan, one
} would need a camera that would capture each line as it is produced.

What you're saying is there must be a system out there that
digitizes that single stream of line scan intensities, then
processes all that data inside the computer as an image as
opposed to trying to digitize the frame buffer.

- John



From daemon Tue May 16 09:16:59 2000



From: Joseph Passero :      jp-at-spacelab.net
Date: Tue, 16 May 2000 10:05:15 -0400
Subject: Re: test the quality of difraction limited microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Moshe,

Try Klarmann Rulings, Inc., they manufacture reticles. If not a stock item they will make one
to your specification.

There URL is http://www.reticles.com

Best Regards

Joseph Passero

mailto:jp-at-spacelab.net


On Tuesday the 16th of May, 2000 at 07:39:23 -0500, moshe_marc-at-gohip.com wrote and posted:

} Email: moshe_marc-at-gohip.com
} Name: moshe marcovitch
}
} Question: Dear sir
} In order to test the quality of difraction limited microscope I am looking
} for reticles that will enable me to produce difraction patterns of about
} 0.2 micron source can you advice where can I find such reticles . Who can
} produce them for me if they are not available comercially .
} Best regards
} Moshe Marcovitch
}
} ---------------------------------------------------------------------------




________________________________________________________
1stUp.com - Free the Web
Get your free Internet access at http://www.1stUp.com



From daemon Tue May 16 09:27:01 2000



From: Vr. Richard Bejsak-Collorado-Mansfeld :      ricardo-at-ans.com.au
Date: Wed, 17 May 2000 00:16:33 +1000
Subject: New website name available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear colleagues
there are new websites names available. It will look like: www.diptera.ws
or www.????.ws more information you can find on www.coleoptera.org in
section {software house}
Keep care and be of good cheer.

Regards

(name) Vratislav Richard Eugene Maria John Baptist
(surname) of Bejsak (Bayshark)-Collorado-Mansfeld

website: http://www.coleoptera.org
listserver: coleoptera on www.egroup.com/group/coleoptera/info.html
Coleoptera - Australia, Tenebrionidae of World
(incl. Lagriinae, Alleculinae)

University of Sydney
The Wentworth Bldg., Box 62
NSW 2006
AUSTRALIA
phone : +61 414 540 465
email: vratislav-at-bigfoot.com
ricardo-at-ans.com.au
(before Ricardo-at-compuserve.com
and ricardo-at-login.cz )

Only after the last tree has been cut down,
only after the last river has been poisoned,
only after the last fish has been caught,
only then will you find that money can not be eaten.'
CREE INDIAN PROPHECY.

Incoming mail is certified Virus Free.
Checked by AVG anti-virus system (http://www.grisoft.com).





From daemon Tue May 16 09:27:06 2000



From: micro-at-ldeo.columbia.edu (Dee Breger)
Date: Tue, 16 May 2000 10:33:14 -0500
Subject: SEM facility managers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Collegues,

In response to the current thread on the problems of facility managers, I
say count me in as being highly interested! If a discussion group does get
together at M&M it would be great to see a report posted on this listserver
for those of us who unfortunately can't attend the meeting. If somebody
could take notes and post them, I for one would be very appreciative.

Thanks!
Dee




From daemon Tue May 16 09:27:08 2000



From: Debby Sherman :      sherman-at-btny.purdue.edu
Date: Monday, May 15, 2000
Subject: Re: starch granules

Contents Retrieved from Microscopy Listserver Archives
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------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Mark,
I have been working with cyanobacteria for over 15 years. We found out in the late 80's that the only way to really hold the starch granules together is by using plunge freezing and freeze substitution techniques. I would substitute in acetone + osmium and embed in Spurr's for normal ultrastructure and use ETOH and embed in HM-20 for immuno.
Check out the following references for details of method and contact me for further explanations:


Schneegart, M.A., D. M. Sherman, S. Nayar, and L. A. Sherman (1994). Oscillating Behavior of Carbohydrate Granule Formation and Dinitrogen Fixation in the Cyanobacterium Cyanothese sp. strain ATCC 51142. J. Bacteriology. 176:1586-1597.

Sherman, D. M., T. Troyan, and L. A. Sherman (1994). Localization of Membrane Proteins in the Cyanobacterium Synechococcus sp. PCC7942. Plant Physiol. 106:251-262

Plunge freezing apparatus can be made in a university shop at relatively low cost and works great for many unicellular organisms.
Debby

Debby Sherman, Manager Phone: 765-494-6666
Microscopy Center in Agriculture FAX: 765-494-5896
Dept. of Botany & Plant Pathology E-mail: sherman-at-btny.purdue.edu
Purdue University
1057 Whistler Building
West Lafayette, IN 47907-1057

--------------------------------------


Hi all - a first attempt at unloading a problem onto the listserver!

I am trying to get thin sections of blue-green algae that are loaded with
starch granules. The problem is that every time we do the preps, we end up
with cells that lose some granules, leaving a gaping hole, or of starch
granules that have holes in the centre. I'm using a standard sort of
method - fix in phosphate + 2.5% glut 2 hours, osmium 1% 2 hours,
dehydrate in ethanol series, finishing with prop. ox, 3:1 prop. ox/resin
around 2 h, 1:1 2 or 3h then another change overnight, a couple of 100%
resin changes for 1/2 to one day, then polymerization 48h/60C. The fixing
and EtOH steps are done on ice, the rest at room temp, the resin
infiltration is done on a rotating mixer. We've tweaked the method around
but nothing seems to work. I've tried using LR White as well, but with no
luck. Can anybody out there help!?

Mark






********************************************
Mark West,
Unidad de Microscopia Electronica,
(Electron Microscopy Unit)
Instituto de Fisiologia Celular,
Universidad Nacional Autonoma de Mexico,
04510 Mexico D.F.

tel (unidad/lab) *(525) 622 5610*
(casa/home) (525) 619 3020
Fax (525) 616 2282
********************************************







From daemon Tue May 16 09:46:31 2000



From: Gib Ahlstrand :      giba-at-puccini.cdl.umn.edu
Date: Tue, 16 May 2000 09:47:59 -0500 (CDT)
Subject: Re: starch granules

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Responding to the message of
{Pine.WNT.4.10.10005151651240.-3843205-100000-at-mwest.ifisiol.unam.mx}
from Mark West {mwest-at-ifcsun1.ifisiol.unam.mx} :
}
Mark,

If you don't really care about seeing the starch, perhaps you could "light
starve" the algae, put them in the dark for some period of time to deplete or
minimize granule size of the starch, without killing them of course. Then
process as usual. We do this with plant samples to avoid the embedding problems
and the resultant holes in the setions that you describe.

Good luck!

Gib

} Hi all - a first attempt at unloading a problem onto the listserver!
}
} I am trying to get thin sections of blue-green algae that are loaded with
} starch granules. The problem is that every time we do the preps, we end up
} with cells that lose some granules, leaving a gaping hole, or of starch
} granules that have holes in the centre. I'm using a standard sort of
} method - fix in phosphate + 2.5% glut 2 hours, osmium 1% 2 hours,
} dehydrate in ethanol series, finishing with prop. ox, 3:1 prop. ox/resin
} around 2 h, 1:1 2 or 3h then another change overnight, a couple of 100%
} resin changes for 1/2 to one day, then polymerization 48h/60C. The fixing
} and EtOH steps are done on ice, the rest at room temp, the resin
} infiltration is done on a rotating mixer. We've tweaked the method around
} but nothing seems to work. I've tried using LR White as well, but with no
} luck. Can anybody out there help!?
}
} Mark
********************************************
} Mark West,
} Unidad de Microscopia Electronica,
} (Electron Microscopy Unit)
} Instituto de Fisiologia Celular,
} Universidad Nacional Autonoma de Mexico,
} 04510 Mexico D.F.
}
} tel (unidad/lab) *(525) 622 5610*
} (casa/home) (525) 619 3020
} Fax (525) 616 2282
} ********************************************

Gib Ahlstrand
Electron Optical Facility, University of Minnesota, Dept. Plant Pathology
495 Borlaug Hall, St. Paul, MN. USA. 55108 (612)625-8249
612-625-9728 FAX, giba-at-puccini.cdl.umn.edu
http://biosci.umn.edu/MIC/consortium.html



From daemon Tue May 16 11:05:58 2000



From: Elaine Humphrey :      ech-at-unixg.ubc.ca
Date: Tue, 16 May 2000 08:39:02 -0700
Subject: Re: SEM facility managers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Me too, though I would like to attend the meeting in Philadelphia.
Elaine
}
}
} Collegues,
}
} In response to the current thread on the problems of facility managers, I
} say count me in as being highly interested! If a discussion group does get
} together at M&M it would be great to see a report posted on this listserver
} for those of us who unfortunately can't attend the meeting. If somebody
} could take notes and post them, I for one would be very appreciative.
}
} Thanks!
} Dee


Dr. Elaine Humphrey
Biosciences Electron Microscopy Facility
University of British Columbia
6270 University Blvd, mail-stop Botany
Vancouver, BC
CANADA, V6T 1Z4
Phone: 604-822-3354
FAX: 604-822-6089
e-mail: ech-at-unixg.ubc.ca




From daemon Wed May 24 20:27:06 2000



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Tue, 23 May 2000 08:19:09 -0500
Subject: Equipment reliability

Contents Retrieved from Microscopy Listserver Archives
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Martyn,

In my experience, two weeks of downtime a year is not bad at all, for any
EM. Especially when you are dealing with many users with varying levels of
expertise and a heavy usage schedule. EM's are maintenance-intensive
instruments (especially TEM's), and even performing preventive maintenance
routines can easily eat up a week a year.

In my opinion, you're doing great.

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/




-----Original Message-----
} From: "HARRISm-at-esm-semi.co.uk"-at-sparc5.microscopy.com
[mailto:"HARRISm-at-esm-semi.co.uk"-at-sparc5.microscopy.com]
Sent: Tuesday, May 23, 2000 5:02 AM
To: Microscopy-at-sparc5.microscopy.com



A how long is a piece of string ? type question


I would be pleased if anyone could broaden my views on equipment
reliability .
Basically we have a 3yr old FESEM which I consider to be fairly
reliable in that it is on call 24hrs/day , has numerous ( non
dedicated users ) and apart from downtime for filament change and the
odd wear and tear type problems answers our needs .
As we do not have a back up instrument and when we do experience
problems it's always at the worse time certain personnel have the
impression that it is unreliable .

What do other sem users expect in terms of reliability , apart from my
' subjective ' comments is it quantifiable , would approx 2wks /year
downtime including planned maintenance be considered excessive ?

Regards
Martyn Harris
harrism-at-esm-semi.co.uk



From daemon Wed May 24 20:27:07 2000



From: Belinda White :      whiteb-at-EMU.UNP.AC.ZA
Date: Tue, 23 May 2000 16:16:39 +0200
Subject: RMC cryo-ultramicrotome contact numbers

Contents Retrieved from Microscopy Listserver Archives
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Please could someone from RMC contact me off-line with contact telephone and fax. numbers.

Many thanks

Belinda



Belinda White
Centre for Electron Microscopy
University of Natal
Private Bag X01
Scottsville
PIETERMARITZBURG
3209
SOUTH AFRICA

email: whiteb-at-nu.ac.za
tel: +27 ( 0)33 2605157
fax: +27 (0)33 2605776



From daemon Wed May 24 20:27:07 2000



From: Larry Allard :      l2a-at-ornl.gov
Date: Tue, 23 May 2000 10:21:00 -0400
Subject: RE: Equipment reliability

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Just another opinion...

Two weeks a year (avg) seems like a lot of downtime for a FE-SEM to
me. Our Hitachi S-800 SEM, for example, is 13 years old, just
recently had only it's second emitter replacement, and, not counting
building air, water and power problems, has certainly had only about
4 weeks of instrument-related downtime in that 13 year period,
including annual PM services.

I guess it's tough to break an anvil... ;-).

Larry
PS it's a heavily used multi-user instrument...




} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Dr. Lawrence F. Allard
Senior Research Staff Member
High Temperature Materials Laboratory
Oak Ridge National Laboratory
1 Bethel Valley Road
Bldg. 4515, MS 6064
PO Box 2008
Oak Ridge, TN 37831-6064

865-574-4981
865-576-5413 Fax
allardlfjr-at-ornl.gov



From daemon Wed May 24 20:27:09 2000



From: MICHAEL DELANNOY :      delannoy-at-mail.jhmi.edu
Date: Tue, 23 May 2000 10:58:37 -0400 (EDT)
Subject: Re: Rapid Fixation of cells for SEM)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Gib,
Have you thought about microwave fixation in the pressence
of aldehyes (para/GA) followed by microwave fixation in osmium.
each takes seconds and fixations are as good or better (for rapid
fixation) than standard fixation. The key is to keep heat off
the sample (use water baths and/or ice bath). The theory is
that the microwave pulsations increase the penetration speed of
the fixatives. Call Ted Pella's tech divison for more info or
protocols.

Mike Delannoy




From daemon Wed May 24 20:27:10 2000



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Tue, 23 May 2000 09:17:16 -0700
Subject: Re: Re[2]:BSE resolution

Contents Retrieved from Microscopy Listserver Archives
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Dear Woody,
I had a problem once that really strained the ultimate Z number sensitivity
of my BSE detector. This was a case of a small amount of a marker chemical,
I think Strontium, being fed to growing fish and trying to detect it in a
scale of the fish. I was definintely able to detect the faint, brighter band
on my GW BSE detector (solid state), but not on my Robinson (scinntilator)
on the other SEM. I had to use more beam current for the GW, but it saw the
contrast when the Robinson didn't. More sensitivity does not mean better Z
resolution.
At 01:36 PM 5/22/00 -0500, you wrote:
}
} Hello Gary,
}
} I have never used a sintillator type BSE detector, but the major differences
} are
} two fold.
}
} Typically (though diodes are getting better all the time) the Robinson has a
} better low energy BSE detection effiency. It follows that for the same
} noise
} level electronics, it would exhibit better sensitivity. The dynamic range
} problem will still exist for very different Zs in the field of view. In the
} middle and upper portions of the sensitivity range (low and lower), I would
} not
} expect much difference between them. ...Any Comments from users of both????
}
} I like the 4 quad diodes since I can go differential mode for macro
} topography
} (like fracture surfaces) and show the gross features while suppressing the
} fine
} detail.
}
} The Pt coating can have a profound negative effect on sensitivity if not
} extremely thin. If given a choice, I would always use carbon for the best
} BSE
} sensitivity. ...If not hazardous, I would use Be, BUT!!!! For elements in
} the
} range of Al & Si, I prefer to use carbon and lower beam voltages to minimize
} penetration, especially if they are films or very small features.
}
} I once presented some data illustrating the BSE signal attenuation as a
} function
} of sputtered Au thickness. But that data would be hard to retrieve now.
}
} Woody White
} McDermott Technology

Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Wed May 24 20:27:10 2000



From: Rosemary Walsh :      rw9-at-psu.edu
Date: Tue, 23 May 2000 12:49:29 -0400
Subject: PtC shadowing question

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Dear Listers,
I now know that the size of the gold used in the label was
0.8 nm and the DNA in another exp. will be ssDNA (7249 bases,
circular). We're prepared to try 1.4 nm and 3.0 nm and darkfield
before shadowing. I have copied the repsonses below and will post
the results of our efforts. Once again, I am most grateful for your help.
Rosemary

I don't think the PT shadowing would obscuring the gold labelling. I did
some rotary shadowing of myosin molecules with antibody attached. You
couldn't see the actual Y structure but you could see arrowheads. If you
can see isolated IgG molecules you should be able to pick up the gold
particles. Patty Jansma

Do you mean you want to see a gold particle in a preparation that is
shadowed with Pt after the gold labelling step has take place? If so,
the answer is probably "yes." The Pt gives such a high-contrast
shadow that it may be difficult to pick out the small gold probe
against the contrasty background. Carol Heckman

NO, it should enhance the whole image so that you can more readily see
exactly where the gold is labelling. Cheers,Marilyn Henderson

I would suggest to use dark field imaging of your labeled DNA-protein
complexes in TEM. By using this technique, you do not need to use Pt-
shadowing of your samples. Best regards from Prague. O. Benada

Rosemary, The typical "grain" size using Pt/C is on the order of 0.8 nm.
So the answer to your question would be dependent on the size of the gold
probe you are using. I would think that you would be OK with a gold probe
larger than 3 nm, but this is speculation on my part, I have not actually
done that exact thing with my own two hands. Chuck (Charles Garber)


Rosemary Walsh, Manager
The Electron Microscope Facility for the Life Sciences,
A Shared Technology Facility, The Life Sciences Consortium
1 South FrearLab
Penn State University
University Park, PA 16802
(814) 865-0212
rw9-at-psu.edu
http://www.lsc.psu.edu/stf/em/home.html




From daemon Wed May 24 20:27:11 2000



From: Steve Chapman :      protrain-at-emcourses.com
Date: Tue, 23 May 2000 15:32:39 +0100
Subject: Sputter Coating

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Hi

Sure it is possible to melt samples by sputter coating but it is usually
when you are using the old fashioned "brute force" systems that use a variac
to adjust the operating voltage.

A big problem with sputter coating is that many of the solutions to one
problem are the reasons for others!

1. To help prevent melting increase the working distance (} 5cms) and cut
down the current (~10mA) - problem this cuts down the coating thickness so I
need to coat for longer!

2. To help prevent melting use a number of short coating periods with a
cooling down period between - problem multi coats build structure on the
specimen and from tests I have conducted the first 10 seconds of the plasma
are the hottest! We actually use the multi coat method to make test
specimens ( 5 x 1 minute coats at 20mA 5cms working distance with 1 minute
between coats).

3. During the early days of SEM sputter coating I would place the sputter
head in a refrigerator for an hour prior to use, part of my method in order
to tray and track down the heat problems. There was considerably less
heating under these circumstances but we were very very careful with
condensation on the then "high voltage" connection. This test led us to
talk very seriously about water cooling the sputter head, a route that was
taken by Baltzers at one stage.

4. My route for a specimen that melts would be to cut down the current,
give a longer coating time and provided you were not working above 5,000X
have a few minutes "cooling time" between no more than three coating
sessions.

Good luck

Steve Chapman
Senior Consultant Protrain
For consultancy and professional training in EM world wide
Tel 44+ 1280 814774 Fax 814007
www.emcourses.com




From daemon Wed May 24 20:27:11 2000



From: Steve Chapman :      protrain-at-emcourses.com
Date: Tue, 23 May 2000 21:20:39 +0100
Subject: FEG Instrument Reliability

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Hi

I have been involved with clients around the world since FEG systems first
became available. I have found this style of equipment to be very reliable
with if anything less down time than the conventional W hairpin instruments.

I must say that I have seen a considerable difference in the performance of
these instruments with a certain manufacturer's range of FEG microscopes
being far less of a problem in the production of very high quality results
than any of the others.

This said if you are only averaging two weeks per year down time there
should be no one within your organisation who should complain.

As a consultant and ex service engineer I can only suggest that people look
at other instruments within your establishment of equal complexity and
compare the up time performances.

If we can help a phone call consultation costs nothing?

Steve Chapman
Senior Consultant Protrain
For consultancy and professional training in EM world wide
Tel 44+ 1280 814774 Fax 814007
www.emcourses.com



From daemon Wed May 24 20:27:13 2000



From: Sally Stowe :      STOWE-at-rsbs.anu.edu.au
Date: Wed, 24 May 2000 09:37:57 +1000
Subject: RE: Equipment reliability

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And another -
same as Larry really, we have a four year-old Hitachi 4500 that has had no
emitter deterioration and probably 5 days total downtime counting
bakeouts. Touch wood. Also in a multiuser facility. But this FESEM series
are proverbially reliable and in general two weeks average per year doesnt
seem excessive, particularly if the FESEM is only three years old, and some
teething problems with any EM column would not be unusual in the first
couple of years. Depends on the context what is acceptable I guess, but if
absolute reliability is a requirement, 24 hr access by "non-dedicated"
users might be the first point to examine?
good luck,
Sally Stowe


} } } Larry Allard {l2a-at-ornl.gov} 05/24/00 12:21am } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Just another opinion...

Two weeks a year (avg) seems like a lot of downtime for a FE-SEM to
me. Our Hitachi S-800 SEM, for example, is 13 years old, just
recently had only it's second emitter replacement, and, not counting
building air, water and power problems, has certainly had only about
4 weeks of instrument-related downtime in that 13 year period,
including annual PM services.

I guess it's tough to break an anvil... ;-).

Larry
PS it's a heavily used multi-user instrument...




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865-574-4981
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From daemon Wed May 24 20:27:13 2000



From: J. Sopp :      jmsopp-at-u.washington.edu
Date: Tue, 23 May 2000 17:37:46 -0700 (PDT)
Subject: gold sample

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hello,

I hope that someone can make a suggestion to me. I am looking for a
sample to use as a standard for low level nanoindentation.

What would be ideal is a gold film of perhaps 1 micron or more on perhaps
polished silicon wafer. This needs to be as smooth as possible, rms
roughness of less than a nanometer. I know that this is quite possible, I
have samples that are just like this but they are too thin (15nm).

does anyone have a suggestion?

thanks,

Jeffrey Sopp




From daemon Wed May 24 20:27:13 2000



From: H. Fong :      hfong-at-u.washington.edu
Date: Tue, 23 May 2000 17:48:56 -0700 (PDT)
Subject: Al peak in EDS spectrum

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We have a JOEL 5200 SEM with a Link Analytical QX 2000 EDS detector. The
problem is that we always get an aluminum peak in every EDS sprectrum
regardless of sample & scan size. Does anyone have any suggestions on why
this is?

Thanks,
Hanson Fong

Univ. of Washington
Materials Science & Engineering Department
Box 352120
Seattle, WA 98195
USA



From daemon Wed May 24 20:27:14 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Tue, 23 May 2000 20:26:44 -0700
Subject: species analysis in real time

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This may be off-topic for this listserver but I'll give it
a shot for those who are into materials science.

Suppose that you have an etching system based
on a small plasma chamber using CF4 and O.
Is there some simple/convenient way to measure
species during the etching process to indicate that some
end point has been reached? i.e., a major etching
area has been etched and the nature of the species
has dramatically or noticeably changed?

I can think of many x-ray methods, but what I am
looking for is basically a sensor of some sort at a
port in the etching chamber system. There is no
SEM beam--and presumedly, no direct or indirect
x-rays.

Any ideas?

gary g.



From daemon Wed May 24 20:27:48 2000



From: John Twilley :      jtwilley-at-sprynet.com
Date: Wed, 24 May 2000 01:07:32 -0400
Subject: Re: Al peak in EDS spectrum

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Hanson,

Are you sure it's Al and not Br? If your accelerating voltage is not high
enough to stimulate the emission of Br K radiation, it can be easy to mistake
the Br L lines for the K lines of Al which are slightly narrower but at the
same peak position. Like the other halogens, Br can initiate corrosion of
metals and end up in the corrosion products. And if this metal is part of
your detector window.....

John Twilley
Art Conservation Scientist

H. Fong wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} We have a JOEL 5200 SEM with a Link Analytical QX 2000 EDS detector. The
} problem is that we always get an aluminum peak in every EDS sprectrum
} regardless of sample & scan size. Does anyone have any suggestions on why
} this is?
}
} Thanks,
} Hanson Fong
}
} Univ. of Washington
} Materials Science & Engineering Department
} Box 352120
} Seattle, WA 98195
} USA





From daemon Wed May 24 20:27:49 2000



From: Jonathan Barnard :      Jonathan.Barnard-at-angstrom.uu.se
Date: Wed, 24 May 2000 09:30:35 +0200
Subject: RE:species analysis in real time

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A change in the absorption spectrum of light could to be used as a cut off.
The photon penetration is of the order of the first few monolayers. The
difficulty is using sources and filters of the right frequency range that
is compatible with the etchants and the material you want to detect (when
it becomes exposed).

You would need to find out the optical reflectivity/absorption of the
materials involved. Is this feasible?
********************************************************
Dr Jonathan Barnard

Analytical Materials Physics
The Angstrom Laboratory, Uppsala University
P O Box 534, SE-751 21 Uppsala, Sweden
Phone: +46-(0)18-4716838 Fax: +46-(0)18-500131
Phone: Microscope room +46 18 471 6365
http://www.angstrom.uu.se/analytical/home.html
********************************************************



From daemon Wed May 24 20:27:49 2000



From: Gunnar Kopstad :      gunnar.kopstad-at-medisin.ntnu.no
Date: Wed, 24 May 2000 09:37:39 +0200
Subject: about: Al peak in EDS spectrum

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Hi Hanson Fong,
A possible explanation is that you have a collimator made of aluminium.
Either the collimator is not properly aligned, or, the carbon paint inside
the collimator has broken. Another explanation can be high energy x-rays
hitting the collimator.

Vennlig Hilsen
dr.ing Gunnar Kopstad
overingeni¿r Avd f Patologi, Rit

tlf. 73 86 86 56, tlf. privat 72 88 79 58
pers.s. 967 75 026



From daemon Wed May 24 20:27:50 2000



From: Ken Converse :      qualityimages-at-netrax.net
Date: Wed, 24 May 2000 06:56:30 -0700
Subject: Re: species analysis in real time

Contents Retrieved from Microscopy Listserver Archives
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Dr. Gary Gaugler wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} This may be off-topic for this listserver but I'll give it
} a shot for those who are into materials science.
}
} Suppose that you have an etching system based
} on a small plasma chamber using CF4 and O.
} Is there some simple/convenient way to measure
} species during the etching process to indicate that some
} end point has been reached? i.e., a major etching
} area has been etched and the nature of the species
} has dramatically or noticeably changed?
}
} I can think of many x-ray methods, but what I am
} looking for is basically a sensor of some sort at a
} port in the etching chamber system. There is no
} SEM beam--and presumedly, no direct or indirect
} x-rays.
}
} Any ideas?
}
} gary g.


Gary,
There are some small, inexpensive mass-specs available that can operate
at pressures up to 25 mT. I believe Ferranti in New Mexico is one that
I've seen. I don't know what pressure your plasma system operates at,
but if it's too high, you'd only need a roughing pump to operate this
system.

When I looked into it a few years ago, it was about $3000 plus a
computer to plug it into.

Ken Converse
owner
Quality Images
Delta, PA



From daemon Wed May 24 20:27:51 2000



From: Ken Converse :      qualityimages-at-netrax.net
Date: Wed, 24 May 2000 07:04:46 -0700
Subject: Re: Al peak in EDS spectrum

Contents Retrieved from Microscopy Listserver Archives
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H. Fong wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} We have a JOEL 5200 SEM with a Link Analytical QX 2000 EDS detector. The
} problem is that we always get an aluminum peak in every EDS sprectrum
} regardless of sample & scan size. Does anyone have any suggestions on why
} this is?
}
} Thanks,
} Hanson Fong
}
} Univ. of Washington
} Materials Science & Engineering Department
} Box 352120
} Seattle, WA 98195
} USA


Hanson,
I'm not familiar with the 5200 chamber geometry, but I'm sure there is
aluminum in there. The question is: can the detector see the aluminum
and can the aluminum be excited by either backscattered electrons or
x-rays generated by from the specimen? The detector doesn't care or
know WHERE the x-rays come from, only that they are being generated and
are within line of sight of the detector. Often, moving the detector
closer to the specimen and making sure that the collimator is properly
placed on the detector nose will help narrow the field of view.

Ken Converse
owner
Quality Images
Delta, PA



From daemon Wed May 24 20:27:51 2000



From: Scott Wight :      scott.wight-at-nist.gov
Date: Wed, 24 May 2000 08:50:41 -0400
Subject: Re: Al peak in EDS spectrum

Contents Retrieved from Microscopy Listserver Archives
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I would check your electron trap and make sure that it is installed
correctly, your detector window is almost certainly coated with Aluminum to
keep light out, electrons striking the window can produce the effect you
are observing. If this is the case you should also be seeing a large hump
in the high end of your continuum. Good luck and let the list know what
you discover.
Scott


}
} We have a JOEL 5200 SEM with a Link Analytical QX 2000 EDS detector. The
} problem is that we always get an aluminum peak in every EDS sprectrum
} regardless of sample & scan size. Does anyone have any suggestions on why
} this is?
}
} Thanks,
} Hanson Fong
}
} Univ. of Washington
} Materials Science & Engineering Department
} Box 352120
} Seattle, WA 98195
} USA


------------------- note: new mailing address ------------------------
Scott Wight fax: 301-417-1321
NIST 222/A113 W voice: 301-975-3949
100 Bureau Dr STOP 8371 | email: scott.wight-at-nist.gov
Gaithersburg, MD 20899-8371 \|/ disclaimer: Any opinion expressed
is my own and does not represent those of my employer.




From daemon Wed May 24 20:27:52 2000



From: Larry Allard :      l2a-at-ornl.gov
Date: Wed, 24 May 2000 09:25:50 -0400
Subject: Re: Sputter Coating

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Ron:

If you use a tride sputter coater, you should not experience any
significant heat build-up during a normal coating process, because
the geometry of the sputter target, employing a central magnet
element to create a shaped magnetic field, directs the electrons in
the plasma away from the sample, thus reducing the "I-square-R"
heating. If you are using an older diode sputter coater, it is a
simple matter to use a pulsed coating process to reduce heating
effects. We used this process many years ago before the triode
sputter coaters were introduced. It turns out that a cycle of 1 sec
ON, 2 sec OFF (or maybe it was 2 ON, 1 OFF) ended up generating an
increase in temperature on an insulating sample only to about 40¡C,
or about body temperature (directly measured using thin-wire
thermocouples). We found we could coat a piece of styrofoam cup with
200 of gold using this process in a diode sputterer, and see no
evidence of melting of the structure.

This is basically the suggestion Steve Chapman makes, to use several
brief coating times. The more regulated, very short heating times
with some cooling time in between seemed to do the trick for us. Try
it, you'll like it... :-).

Larry
PS I think we published this somewhere...if I find it, I'll post.





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Dr. Lawrence F. Allard
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Oak Ridge National Laboratory
1 Bethel Valley Road
Bldg. 4515, MS 6064
PO Box 2008
Oak Ridge, TN 37831-6064

865-574-4981
865-576-5413 Fax
allardlfjr-at-ornl.gov



From daemon Wed May 24 20:27:53 2000



From: Anthony Garratt-Reed :      tonygr-at-mit.edu
Date: Wed, 24 May 2000 09:31:50 -0400
Subject: Re: Al peak in EDS spectrum

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How big is the Al peak compared to other peaks? Does it vary according to
the sample composition? Do you get it even if there is no sample in place?
Are you sure you have the sample height correct (the "nominal" height is
often not right - you need to check the X-ray count rate vs. sample
height)? Are you certain it is Al (the position is accurate, the peak
width right)? Do you always use the same voltage, or is the peak there at
different beam voltages? Do you have a thin window or Be detector? Is the
detector working normally in all other respects? Has this spurious
response always been present (i.e. since installation in the '80's) or have
you only recently observed it?

Sorry to ask these questions, but they are relevant.

If there really is an Al peak, it means that within the detector's field of
view is something made primarily of Al which is being irradiated either
with x-rays or electrons. Assuming the working distence (sample height) is
correct, this implies some problem with the collimator, because it's
purpose is to eliminate exactly this type of spurious x-ray signal. You do
have your original collimator and electron trap, do you? Has it been
damaged or moved in some accident with the sample stage?

If the Al signal varies strongly with sample composition (for example, much
weaker with a carbon sample than with a tungsten sample) then it could well
be related to backscattered electrons or secondary x-ray flourescence.

If, on the other hand, the signal varies strongly with operating voltage
(especially if the peak moves) then it probably isn't Al at all, but a
spurious response related either to electrons getting through the window or
to electron noise.

No solutions here (and some of my thoughts are included for completeness as
this is a positing going out for everyone to read), but I hope my musings
help.

Tony Garratt-Reed.



At 05:48 PM 05/23/2000 -0700, you wrote:
} ------------------------------------------------------------------------
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** Anthony J. Garratt-Reed
** MIT Room # 13-1027
** 77 Massachusetts Avenue
** Cambridge, MA 02139-4307
** USA
**
** Phone: (+) 1-617-253-4622
** Fax: (+) 1-617-258-6479
**




From daemon Wed May 24 20:27:53 2000



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Wed, 24 May 2000 08:07:08 -0600
Subject: species analysis in real time

Contents Retrieved from Microscopy Listserver Archives
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Gary,

I think there are a few ways of doing that:

1) You should be able to get some spectral data from the plasma itself.
Hook up a spectrometer and you should be able to see the components in
the spectrum. You may have to excite the plasma with some light.

2) Hook up a mass spectrometer (quadrupole). That should be able to give
you masses of the components.

Don't people do that on a regular basis? You may check the web or
literature for "residual gas analysis" or similar.

Michael

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Dr. Gary Gaugler [mailto:gary-at-gaugler.com]
Sent: Tuesday, May 23, 2000 9:27 PM
To: MSA listserver
Cc: just_in_case_I_bounce


This may be off-topic for this listserver but I'll give it
a shot for those who are into materials science.

Suppose that you have an etching system based
on a small plasma chamber using CF4 and O.
Is there some simple/convenient way to measure
species during the etching process to indicate that some
end point has been reached? i.e., a major etching
area has been etched and the nature of the species
has dramatically or noticeably changed?

I can think of many x-ray methods, but what I am
looking for is basically a sensor of some sort at a
port in the etching chamber system. There is no
SEM beam--and presumedly, no direct or indirect
x-rays.

Any ideas?

gary g.



From daemon Wed May 24 20:27:53 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Wed, 24 May 2000 07:54:58 -0700
Subject: Re: species analysis in real time

Contents Retrieved from Microscopy Listserver Archives
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This is the same thing as I am trying to do. In my case, the passivation
will be either sinox or PSG. At present, I remove them using two methods.
One is to do a short soak in BOE, rinse & dry. Then plasma etch with
CF4 at about 80 mTorr. Power and gas flow have dramatic effects on
etch rate. Same for dilution of BOE. The challenge is to nicely get
through the passivation and stop at the uppermost SiO2 dry ox layer.

The physical size of my specimens is small. A 3" diameter cylindrical
chamber would be fine. I use a sputter coater now in etch mode (quartz
chamber of course). It seems to me that there would not be much
of a change from a detection/measuring system after the process
finishes off the passivation and reaches the SiO2. Maybe not true.

There have been several good suggestions on the list so far. One I
will check out right away is the residual gas analyzer. I also may
need some different type of etching unit rather than the coater
operating in etch mode. Since I am interested in FA too, the
specimens are too small to justify the cost of impressive huge
etching units.

gary

At 06:27 AM 5/24/00, you wrote:
} Hello Gary,
} I am also interested in this. We make PLD and I work in the FA group. Being
} this as it may I am new to this field and would wish to find a technique to
} get through the passivation and intrametal layers.
} Thank you for any help.
}
} Sincerely,
} Robb Westby
} Associate Reliability Engineer
} Lattice Semiconductor
}
} "Dr. Gary Gaugler" wrote:
}
} } This may be off-topic for this listserver but I'll give it
} } a shot for those who are into materials science.
} }
} } Suppose that you have an etching system based
} } on a small plasma chamber using CF4 and O.
} } Is there some simple/convenient way to measure
} } species during the etching process to indicate that some
} } end point has been reached? i.e., a major etching
} } area has been etched and the nature of the species
} } has dramatically or noticeably changed?
} }
} } I can think of many x-ray methods, but what I am
} } looking for is basically a sensor of some sort at a
} } port in the etching chamber system. There is no
} } SEM beam--and presumedly, no direct or indirect
} } x-rays.
} }
} } Any ideas?
} }
} } gary g.



From daemon Wed May 24 20:27:54 2000



From: Donald O'Leary :      donoleary-at-worldnet.att.net
Date: Wed, 24 May 2000 12:07:07 -0400
Subject: Chemical Microscopy Workshop, LM

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The weekend workshop on Chemical Microscopy, sponsered by the New York
Microscopical Society, originally sheduled for the weekend of May 20 has
been rescheduled for the weekend of June 17 & 18.

This is an opportunty to learn some of the fundamentals of Chemical
Microscopy from Skip Panenik of Trace Analysis.

The course will be held in West Paterson, NJ.

For further information contact Don O'Leary
Phone (201) 797-8849 Fax (425) 988-1415
E-mail donoleary-at-worldnet.att.net

Visit the NYMS website at www.nyms.org

Don O'Leary
Education Chairman , NYMS



From daemon Wed May 24 20:27:54 2000



From: Ronald Vane :      RVaneXEI-at-concentric.net
Date: Tuesday, May 23, 2000 9:45 PM
Subject: species analysis in real time

Contents Retrieved from Microscopy Listserver Archives
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Dear Dr. Guagler and listers:

I am looking at this same problem for my own Plasma cleaning process. I just
purchased a small, fiber optic, emission spectrometer (USB 2000) from OCEAN
OPTICS to examine the light coming from my cleaning plasma. I just setup the
software yesterday and will be trying it today. If I get some end point
results I will repost to this thread in a few days. There is plasma etch
literature that suggests that end points can be observed in the plasma
emission lines.

Ronald Vane
XEI Scientific
(650) 369-0133

-----Original Message-----
} From: Dr. Gary Gaugler {gary-at-gaugler.com}
To: MSA listserver {Microscopy-at-sparc5.microscopy.com}
Cc: just_in_case_I_bounce {zaluzec-at-sparc5.microscopy.com}



From daemon Wed May 24 20:27:54 2000



From: Ronald Vane :      RVaneXEI-at-concentric.net
Date: Wednesday, May 24, 2000 2:26 AM
Subject: about: Al peak in EDS spectrum

Contents Retrieved from Microscopy Listserver Archives
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Al is present everywhere in the SEM. X-rays are produced by emission
(electrons hitting the material) and Fluorescence (X-rays hitting the
material). Collimator fluorescence is a major design problem for EDS system
designers. High Z materials for stopping X-rays fluoresce strongly, and Low
Z materials have no stopping power. Collimators often have high Z material
lined with Aluminum to stop X-rays and then filter out the Fluorescence. If
some of the AL is displaced it can be excited by either stray electrons or
x-rays and cause the Al peak you see.

Ronald Vane
XEI Scientific


-----Original Message-----
} From: Gunnar Kopstad {gunnar.kopstad-at-medisin.ntnu.no}
To: Microscopy-at-sparc5.microscopy.com {Microscopy-at-sparc5.microscopy.com}



From daemon Wed May 24 20:27:55 2000



From: phil.swab-at-depsci.com (Phil Swab)
Date: Wed, 24 May 2000 09:56:49 -0700
Subject: Al peak in EDS spectrum

Contents Retrieved from Microscopy Listserver Archives
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H. Fong,

On many scopes I've found this was an artifact of an aluminum sample
holder. Painting the surface of the sample holder with colloidal graphite
or using a carbon planchet usually eliminates the problem.

Phil Swab
Engineering Development
Deposition Sciences Inc.
Santa Rosa, CA
707-566-3718
phil.swab-at-depsci.com

-----Original Message-----
} From: H. Fong [SMTP:hfong-at-u.washington.edu]
Sent: Tuesday, May 23, 2000 5:49 PM
To: microscopy-at-sparc5.microscopy.com




We have a JOEL 5200 SEM with a Link Analytical QX 2000 EDS detector. The
problem is that we always get an aluminum peak in every EDS sprectrum
regardless of sample & scan size. Does anyone have any suggestions on why
this is?

Thanks,
Hanson Fong

Univ. of Washington
Materials Science & Engineering Department
Box 352120
Seattle, WA 98195
USA





From daemon Wed May 24 20:27:55 2000



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Wed, 24 May 2000 11:42:44 -0700
Subject: Re: Al peak in EDS spectrum

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Hanson,
I solved that problem by cutting a circle of thin lead (Pb) foil to line the
inside of my Al collimator. Poke a hole in the foil for the hole in the
collimator.
At 05:48 PM 5/23/00 -0700, you wrote:

}
}
}
} We have a JOEL 5200 SEM with a Link Analytical QX 2000 EDS detector. The
} problem is that we always get an aluminum peak in every EDS sprectrum
} regardless of sample & scan size. Does anyone have any suggestions on why
} this is?
}
} Thanks,
} Hanson Fong
}
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Wed May 24 20:27:56 2000



From: Rosemary White :      Rosemary.White-at-pi.csiro.au
Date: Thu, 25 May 2000 08:05:01 +1000
Subject: Re: Al peak in EDS spectrum

Contents Retrieved from Microscopy Listserver Archives
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Dear Hanson,

Could it possibly be that your gold target has worn through to the Al
support? This has happened in our cryochamber before.

cheers,


Rosemary White
Microscopy Centre
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia

phone 61-2-6246 5475
fax 61-2-6246 5000
email r.white-at-pi.csiro.au




From daemon Wed May 24 20:27:56 2000



From: Emmanuelle diTomaso :      ditomaso-at-sherman.mgh.harvard.edu
Date: Wed, 24 May 2000 19:13:38 -0400
Subject: Technician position available

Contents Retrieved from Microscopy Listserver Archives
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Edwin Steele Laboratory
at
Massachusetts General Hospital
is actively recruiting a
Lab Technician/Research Assistant
with outstanding background in Histology

The Edwin L. Steele Laboratory (MGH/HMS) is committed to improving
the detection and treatment of cancer through a better understanding
of tumor pathophysiology and the molecular and cellular transport
barriers within tumors.

We are actively recruiting a lab technician/research assistant who
has experience in histology, immunostaining at light and electron
microscopy levels and in situ hybridization.

For more information please see our website: http://steele.mgh.harvard.edu

Please send your resume and 3 letters of recommendation to
Dr. E. di Tomaso via email : ditomaso-at-steele.mgh.harvard.edu
or fax : (617) 726-4172.



From daemon Wed May 24 20:27:58 2000



From: Linda Fox :      LFOX1-at-wpo.it.luc.edu
Date: Wed, 24 May 2000 16:48:12 -0500
Subject: digital and Koehler

Contents Retrieved from Microscopy Listserver Archives
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Hello Friends,
Does anyone know if one needs to make any adjustments/compromises to Koehler illumination when using a digital camera? We are trying out a few demo's and get circular patterns or edge unevenness at optimum Koehler. If the condenser is defocused things are better...but...isn't the entire purpose of Koehler to get the most out of Abbe's equation that we can?? How much of a loss of resolution can be expected without optimum condenser settings.
My sense is that one should always go with great scope alignment. I am just checking to see if any of us old microscopists can be taught a thing or two regarding digital camera set up.
Thanks,
Linda Fox
lfox1-at-wpo.it.lumc.edu



From daemon Wed May 24 20:27:58 2000



From: Anaspec :      anaspec-at-icon.co.za
Date: Wed, 24 May 2000 21:37:02 +0200
Subject: Re: Re[2]:BSE resolution

Contents Retrieved from Microscopy Listserver Archives
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Hi
We have a client on an older Leo S200 with the standard solid state BSD
fitted who has to look at slag off their stainless steel plant.
In this slag there are slight compositional differences which they can only
define should they run their filament on first peak. Strange but true!
At first peak the slightest difference can be seen very easily, at
saturation not a chance.
Now I remember that Steve Chapman did give us an explanation for this the
first time we mentioned it but, alas age catches up on me too and I have
forgotten what it was.

Point is, try this on your system. It works for them. Better resolution on
the BSD at first peak than at saturation.

Cheers
Luc Harmsen
Anaspec, South Africa
Technical support on microscopy.
www.anaspec.co.za

Remember, ICEM 15 will be in
2002, Durban, South Africa.
www.icem15.com


-----Original Message-----
} From: Mary Mager [mailto:mager-at-interchange.ubc.ca]
Sent: Tuesday, May 23, 2000 6:17 PM
To: White, Woody N
Cc: Microscopy-at-sparc5.microscopy.com


Dear Woody,
I had a problem once that really strained the ultimate Z number sensitivity
of my BSE detector. This was a case of a small amount of a marker chemical,
I think Strontium, being fed to growing fish and trying to detect it in a
scale of the fish. I was definintely able to detect the faint, brighter band
on my GW BSE detector (solid state), but not on my Robinson (scinntilator)
on the other SEM. I had to use more beam current for the GW, but it saw the
contrast when the Robinson didn't. More sensitivity does not mean better Z
resolution.
At 01:36 PM 5/22/00 -0500, you wrote:
}
} Hello Gary,
}
} I have never used a sintillator type BSE detector, but the major
differences
} are
} two fold.
}
} Typically (though diodes are getting better all the time) the Robinson has
a
} better low energy BSE detection effiency. It follows that for the same
} noise
} level electronics, it would exhibit better sensitivity. The dynamic range
} problem will still exist for very different Zs in the field of view. In the
} middle and upper portions of the sensitivity range (low and lower), I would
} not
} expect much difference between them. ...Any Comments from users of both????
}
} I like the 4 quad diodes since I can go differential mode for macro
} topography
} (like fracture surfaces) and show the gross features while suppressing the
} fine
} detail.
}
} The Pt coating can have a profound negative effect on sensitivity if not
} extremely thin. If given a choice, I would always use carbon for the best
} BSE
} sensitivity. ...If not hazardous, I would use Be, BUT!!!! For elements in
} the
} range of Al & Si, I prefer to use carbon and lower beam voltages to
minimize
} penetration, especially if they are films or very small features.
}
} I once presented some data illustrating the BSE signal attenuation as a
} function
} of sputtered Au thickness. But that data would be hard to retrieve now.
}
} Woody White
} McDermott Technology

Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca




From daemon Thu May 25 07:18:01 2000



From: Said Mansour :      Said_Mansour-at-ocli.com
Date: Wed, 24 May 2000 20:28:49 -0500
Subject: open microscopy position

Contents Retrieved from Microscopy Listserver Archives
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Hi All,

Optical Coating Laboratory, Inc. (OCLI) a JDS-Uniphase company is looking to
fill a position in the microscopy area in its Analytical Laboratory . The
candiddate is to have an MS or BS degree in materials science, physics or
related areas with experience in AFM , Light microscopy, FTIR,
interferometry...experience in SEM and other electron microscopy, surface
chemistry analysis is a plus. The assumption of the position is immediate. OCLI
is located in Santa Rosa, California, a leader in thin film products for the
telecommunication industry, counter-feiting applications, photonics and a
variety of other products (please visit OCLI's web site:
http://www.ocli.com/career_opps/index.htm, for more information about the open
position and OCLI and its products.)
If you or any microscopist you know is interested, please send your resume to
Human Resources, Att: Earl Jensen or to me directly by responding to this email
or to my address:
Said A. Mansour
2789 Northpoint Parkway
MS 274-3
Santa Rosa, CA 95407

Thank you




From daemon Thu May 25 07:18:01 2000



From: Ben Craft :      bcraft-at-uci.edu
Date: Wed, 24 May 2000 19:22:19 -0700 (PDT)
Subject: Sound Proofing room

Contents Retrieved from Microscopy Listserver Archives
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Our SEM room is a little too noise for high res SEM work, so the service
engineering recommended to dampen the noise. I know there is panels I can
put up on the wall to do this. What is the cheapest solution for doing
this?



#######
#####\_O -Ben Craft-
####/\/}
#### /"
### \





From daemon Thu May 25 07:18:02 2000



From: CraigTbird-at-aol.com
Date: Wed, 24 May 2000 23:09:16 EDT
Subject: RE: AL Peaks in EDS Spectrum

Contents Retrieved from Microscopy Listserver Archives
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Hanson,

I have worked on Noran Systems for 10 years. I have seen this problem several
times and always on a JEOL SEM. Most times it was not the detector. It
usually was a problem with alignment of the beam. The obvious point here is
that detectors do not produce x-rays, they detect them.

Regards,

Craig Theberge



From daemon Thu May 25 07:18:03 2000



From: Gordon Couger :      gcouger-at-rfdata.net
Date: Thu, 25 May 2000 00:53:32 -0500
Subject: Re: digital and Koehler

Contents Retrieved from Microscopy Listserver Archives
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} From: "Linda Fox" {LFOX1-at-wpo.it.luc.edu}

} Hello Friends,
} Does anyone know if one needs to make any adjustments/compromises to
Koehler illumination when using a digital camera? We are trying out a few
demo's and get circular patterns or edge unevenness at optimum Koehler. If
the condenser is defocused things are better...but...isn't the entire
purpose of Koehler to get the most out of Abbe's equation that we can?? How
much of a loss of resolution can be expected without optimum condenser
settings.
} My sense is that one should always go with great scope alignment. I
am just checking to see if any of us old microscopists can be taught a thing
or two regarding digital camera set up.
.} } } } } } } } } } } } } } } } } } .

You are giving up a lot of resolution with a digital camera due to the
pixel spacing so the loss of resolution due to non optimal illumination
have little effect on the image quality.

You can also solve the problem by increasing the distance from
the CCD to the eyepeice so the ragged edge doesn't fall on the
the CCD. This would also reduce the loss of resolution due to
the spacing of the pixels. Of course is aslo decreases the coverage
of the image.

Gordon

Gordon Couger gcouger-at-couger.com

Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00




From daemon Thu May 25 07:18:03 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Wed, 24 May 2000 23:26:13 -0700
Subject: RE: Equipment reliability

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My Amray 1910 FESEM is only used by me. It is up as long
as I say so (don't shut it down, put it in failsafe mode, etc.).
PM takes about one day and this is done per contract twice
a year. The only real bummer is when all 3 apertures have
gone bad, one-by-one over time. Vent, pull the holder, change out
the apertures and evacuate. This takes me about 45 minutes
to accomplish. Each aperture typically lasts about 2 months.
Since Amray gold flashes them, they cannot be flamed. Guess
that is why they call them "consumables."

The only major down time I experienced was with my 1830 load
lock system. The Balzers 240 turbo was going out (high frequency
oscillation). That took about 3 days to fix for a total pump exchange.
Other than this, both systems are very easy to keep running.

If they were in a mixed user environment, I'd opt for the FESEM
over the LaB6. The FESEM is rather tough to screw up....unless
of course, someone really worked at it.

gary g.



At 04:37 PM 5/23/00, you wrote:
} [snip]
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} }
} } A how long is a piece of string ? type question
} }
} }
} } I would be pleased if anyone could broaden my views on equipment
} } reliability .
} } Basically we have a 3yr old FESEM which I consider to be fairly
} } reliable in that it is on call 24hrs/day , has numerous ( non
} } dedicated users ) and apart from downtime for filament change and
} the
} } odd wear and tear type problems answers our needs .
} } As we do not have a back up instrument and when we do experience
} } problems it's always at the worse time certain personnel have the
} } impression that it is unreliable .
} }
} } What do other sem users expect in terms of reliability , apart from
} my
} } ' subjective ' comments is it quantifiable , would approx 2wks
} /year
} } downtime including planned maintenance be considered excessive ?
} }
} } Regards
} } Martyn Harris
} } harrism-at-esm-semi.co.uk
}
} Dr. Lawrence F. Allard
} Senior Research Staff Member
} High Temperature Materials Laboratory
} Oak Ridge National Laboratory
} 1 Bethel Valley Road
} Bldg. 4515, MS 6064
} PO Box 2008
} Oak Ridge, TN 37831-6064
}
} 865-574-4981
} 865-576-5413 Fax
} allardlfjr-at-ornl.gov



From daemon Thu May 25 07:18:04 2000



From: J.Bruyntjes-at-voeding.tno.nl
Date: Thu, 25 May 2000 08:32:54 +0200
Subject: unsubscribe

Contents Retrieved from Microscopy Listserver Archives
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unsubscribe



From daemon Thu May 25 07:18:04 2000



From: Ron Doole :      ron.doole-at-materials.oxford.ac.uk
Date: Thu, 25 May 2000 08:12:38 +0100 (GMT Daylight Time)
Subject: Re: Sound Proofing room

Contents Retrieved from Microscopy Listserver Archives
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Hi Ben,

Assuming that the noise is being generated from equipment
in the room then curtains will reduce the noise level quite
effectively.

Ron

On Wed, 24 May 2000 19:22:19 -0700 (PDT) Ben Craft
{bcraft-at-uci.edu} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Our SEM room is a little too noise for high res SEM work, so the service
} engineering recommended to dampen the noise. I know there is panels I can
} put up on the wall to do this. What is the cheapest solution for doing
} this?
}
}
}
} #######
} #####\_O -Ben Craft-
} ####/\/}
} #### /"
} ### \
}
}
}
}

----------------------
Mr. R.C. Doole
Department of Materials,
University of Oxford.
Parks Road, Oxford. OX1 3PH. UK.
Phone +44 (0) 1865 273701
Fax +44 (0) 1865 283333
ron.doole-at-materials.ox.ac.uk



From daemon Thu May 25 07:18:05 2000



From: Jenichen :      Jenichen-at-proscan.de
Date: Thu, 25 May 2000 10:42:20 +0200
Subject: Re: new developments in imaging systems?

Contents Retrieved from Microscopy Listserver Archives
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Radostin Danev schrieb:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Dear Sergey and others,
}
} I want to add my 2 cents as I like the topic.
} Most of this is a result of my experience with our TEM 2Kx2K CCD.
} We are working in bright field - so I cannot comment on dark field
} performance
} Several points:
}
} 1. The CCD is much more convenient - you get your pictures instantly.
}
} 2. CCD is linear and has larger dynamic range than the film.
}
} 3. The bad thing about the CCD is resolution - about 4 times lower than that
} of the film (in our camera the pixel size is 30 microns).

If You would choose a CCD with smaller pixel size You would get a better
resolution.

} So if you want to
} work in minimum dose you will do better with film.

Never, a good CCD is much more sensitive than a film.

} As I know the CCD is not
} performing well in terms of signal to noise at low doses (and if you have to
} work at 4 times higher magnification because of the resolution the things
} become much worse).

see above , a smaller pixel gives a better sensitivity and a better resolution.

}
}
} 4. The CCD has smaller observation area - again loss of information.

use the so called Image mounting, than You get very large images with much more
image information due to the larger dynamic range and better sensitivity.

}
}
} 5. I don't know about the detection efficiency compared to the film - it
} depends on the thickness of the phosphorous and the accelerating voltage. If
} a photon reaches the CCD chip it will be detected ... the problems are in
} the conversion electron-photon.

The currently leading CCD systems reach single electron sensitivity at thin
phosphor screens and good resolution.

} There are two sides - if you make the
} phosphorous thicker you will get higher detection efficiency but the point
} spread also increases so always a compromise is made between detection
} efficiency and resolution. When I say detection efficiency this is not only
} related to the detection of single electrons (as it detects single
} electrons) but more to the actual signal detected on the background of the
} noise. Apart from the shot noise additional noise is added due to the
} scintillator driven detection and thermal noise in the CCD chip.

In good, highly sensitive CCD systems the Poisson noise of the incoming signal
is dominating, not the CCD noise.

}
}
} The CCDs are now very popular in diffraction work because of the dynamic
} range and linearity.
}
} Here is one reference where a nice comparison between 2Kx2K CCD and film has
} been made:
}
} Downing, K., Hendrickson, F., Ultramicroscopy, 75, (1999), 215-233

Thanks for that.

}
}
} Best regards,
}
} Rado
}
} ---------------------------------------------------------------------
} Radostin Danev
} Laboratory of Ultrastructure Research
} National Institute for Physiological Sciences
} Myodaiji-cho, Okazaki 444-8585, JAPAN
} e-mail: rado-at-nips.ac.jp
} ---------------------------------------------------------------------

--
Best regards / Mit freundlichen Gruessen
Dr. Frank Jenichen
Proscan elektronische Systeme GmbH
Tel.: +49 8195 999 -511 Fax: -512
mailto:Jenichen-at-proscan.de
------------------------------------------------------------
More information concerning our products
and services can be found on our website
http://www.proscan.de




From daemon Thu May 25 07:18:05 2000



From: Van Osta, Peter [JanBe] :      PVOSTA-at-janbe.jnj.com
Date: Thu, 25 May 2000 10:54:51 +0200
Subject: Cellular translocation, possible artefacts

Contents Retrieved from Microscopy Listserver Archives
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Hi,

I have a question about the study of for instance the translocation of a
fluorescent labeled cellular component from the cytoplasm to the nucleus. At
first it seems that labeling the component you want to study and do a
counterstain for the nucleus would be sufficient to give an idea of the
migration of a componenent from the cytoplasm to the nucleus or not.

By doing the experiment this way however you do not have a clue about the
total cell content, because the cytoplasm is not counterstained with a
background stain to show the cell outlines to give an idea of the actual
cell extent. Without a cytoplasm stain, you have no idea of the actual size
of the cell in which the label for the cellular componenent resides. For an
"absolute" idea of the migration I think a background cellular counterstain
is necessary ?

Also the nuceus is thicker than the cytoplasm, so for a given focuslevel
inside the nucleus there is more light falling in the lens form above and
below than in the cytoplasm, which probably will give a non-linear response
curve for the quantification of the translocation ?

Regards,

Peter Van Osta



From daemon Thu May 25 07:18:05 2000



From: Geoff Williams :      Geoffrey.Lloyd.Williams-at-cmich.edu
Date: Thu, 25 May 2000 08:16:57 -0400
Subject: Re: Sound Proofing room

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


A clear case for doing the experiment with a fluorescent probe with
a confocal or multiphoton microscope!
Either way, out-of-focus contributions to intensity are not important.
Your confocal image is also a sample from a well-defined volume of
cell or tissue. Therefore, provided you can regard each
compartment as homogeneously labelled the total compartment
(e.g. cytoplasm, nucleus) volume does not need to be determined.

} From: "Van Osta, Peter [JanBe]" {PVOSTA-at-janbe.jnj.com}
To: Microscopy-at-sparc5.microscopy.com


McMaster-Carr has some of the best selection for sound deadening material and
generally a better price than specialty dealers or other distributors.
Their Web site is very functional http://www.mcmaster.com
Delivery has always been more than prompt and they have more indespensible
items for any microscope lab. No lab should go without one of their catalogs.

Sound control products are in my catalog on page 2777-2779 products ranging
from flat foam to sculptured foam to "sono-tech" foam to acoustical quilts to
acoustical cylinders. Looking through this stuff isn't cheap but of the
products I have seen offered other places the prices here are competitive.

Of course you could always head to a carpet store and dig through their
dumpsters for throw-away remnants and hang them on the walls in the scope
room. Two or three layers might work well enough - not sure about the smell
though. . .

Good luck
Geoff

Ben Craft wrote:

} Our SEM room is a little too noise for high res SEM work, so the service
} engineering recommended to dampen the noise. I know there is panels I can
} put up on the wall to do this. What is the cheapest solution for doing
} this?
}
} #######
} #####\_O -Ben Craft-
} ####/\/}
} #### /"
} ### \

--
Geoff Williams,

Electron Microscope Facility Supervisor
Biology Department
Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859

Geoffrey.Lloyd.Williams-at-cmich.edu
517 774-3576
517 774-3462 (fax)




From daemon Thu May 25 16:29:50 2000



From: Debby Sherman :      sherman-at-btny.purdue.edu
Date: Wednesday, May 24, 2000
Subject: Fwd: digital and Koehler

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Linda,
Look for a digital camera that allows you to capture an image of the illumination pattern with your specimen slide removed from the beam path. This "background" image should then be automatically subtracted from your final image. This will eliminate not only uneven illumination but also small light distortions due to dirt on lenses (that you cannot get off by cleaning external surfaces), etc. Using this feature permits good Koehler illumination and very even illumination on your final image file.
As an example, the SPOT RT software has a feature called "Flatscreen". I capture images from each objective after checking for proper Koehler illumination. These are stored and easily called up as needed. However, the microscope alignment should still be rechecked prior to capturing images.
This is a very important feature if you do Nomarski/DIC imaging. You can easily smooth out the very directional illumination pattern by capturing the lighting pattern without the sample and then subtracting it automatically when capturing the sample image.

Debby

Debby Sherman, Manager Phone: 765-494-6666
Microscopy Center in Agriculture FAX: 765-494-5896
Dept. of Botany & Plant Pathology E-mail: sherman-at-btny.purdue.edu
Purdue University
1057 Whistler Building
West Lafayette, IN 47907-1057

--------------------------------------


Hello Friends,
Does anyone know if one needs to make any adjustments/compromises to Koehler illumination when using a digital camera? We are trying out a few demo's and get circular patterns or edge unevenness at optimum Koehler. If the condenser is defocused things are better...but...isn't the entire purpose of Koehler to get the most out of Abbe's equation that we can?? How much of a loss of resolution can be expected without optimum condenser settings.
My sense is that one should always go with great scope alignment. I am just checking to see if any of us old microscopists can be taught a thing or two regarding digital camera set up.
Thanks,
Linda Fox
lfox1-at-wpo.it.lumc.edu





From daemon Thu May 25 16:29:51 2000



From: DrJohnRuss-at-aol.com
Date: Thu, 25 May 2000 11:02:54 EDT
Subject: Re: digital and Koehler

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In a message dated 5/25/00 10:26:43 AM, sherman-at-btny.purdue.edu writes:

} Look for a digital camera that allows you to capture an image of the
} illumination pattern with your specimen slide removed from the beam path.
} This "background" image should then be automatically subtracted from your
} final image. This will eliminate not only uneven illumination but also
} small light distortions due to dirt on lenses (that you cannot get off
} by cleaning external surfaces), etc. Using this feature permits good Koehler
} illumination and very even illumination on your final image file.

One additional note. Using a background image captured with a log-response
camera (e.g., a Vidicon) does call for subtraction. For a linear response
camera (most CCDs unless you are using some built-in gamma circuitry) you
want to divide by the background (ratio of signal to background). Also, the
problem with this method is that it uses some of your dynamic range, so you
effectively cannot handle as great a range from bright to dark.



From daemon Thu May 25 16:29:53 2000



From: Becky Holdford :      r-holdford-at-ti.com
Date: Thu, 25 May 2000 11:23:15 -0500
Subject: Re: Sound Proofing room

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Ben:Ê we had the same problem in in our SEM/FIB rooms.Ê We got large sheets of
egg-crate foam and glued them to the walls.Ê It gives the place sort of a
"rubber room" appearance, but it works really well.

Ben Craft wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} ToÊ Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Our SEM room is a little too noise for high res SEM work, so the service
} engineering recommended to dampen the noise.Ê I know there is panels I can
} put up on the wall to do this. What is the cheapest solution for doing
} this?
}
} #######
} #####\_OÊÊÊÊÊÊÊÊÊÊÊ -Ben Craft-
} ####/\/}
} #### /"
} ###Ê \

--
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Becky HoldfordÊ (r-holdford-at-ti.com)
972-598-1291 (pager)
KFAB Physical Analysis Labs--SEM/FIB/FA
Kilby Center West
Texas Instruments, Inc.
Dallas, TX
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Ê



From daemon Thu May 25 16:29:53 2000



From: David Doe :      ddoe-at-foma.wsc.ma.edu
Date: Thu, 25 May 2000 12:17:54 -0400
Subject: LM- Manual for fluorescence microscope

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I received an AO fluorescence microscope from a kind colleague but need
to find an instruction manual for it. The microscope is an AO model 10
or 20 and it has a vertical fluorescence illuminator (model 2071)
attached. Also, I'm looking for a trinocular head for the microscope to
do photography. Any assistance in locating these would be greatly
appreciated.

David A. Doe
--
Dr. David A. Doe
Biology Department
Westfield State College
Westfield, MA 01086
413/572-5291
fax: 413-562-3613




From daemon Thu May 25 16:29:55 2000



From: Steve Chapman :      protrain-at-emcourses.com
Date: Thu, 25 May 2000 17:07:24 +0100
Subject: BSE Information

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Hi

Yes Luc is right I did give him an explanation.

The amount of backscatter generated from a specimen depends on the kV, the
probe size and the composition of the material under investigation. If the
level of backscatter is insufficient under "normal" saturation conditions
i.e. the gun is correctly saturated, then by de-saturating the larger source
will result in a larger probe dimension on the specimen; increasing the
probe diameter increases the volume of material involved in the production
of BSE.

Steve Chapman
Senior Consultant Protrain
For consultancy and professional training in EM world wide
Tel 44+ 1280 814774 Fax 814007
www.emcourses.com



From daemon Thu May 25 16:29:56 2000



From: hpadams :      hpadams-at-bcm.tmc.edu
Date: Thu, 25 May 2000 13:29:51 -0500
Subject: RE: new developments in imaging systems?

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I have been following off and on this discussion on CCD cameras for EM.
Much of this discussion has been concerned with "sensitivity". I am being
naive here, but when we talk about "sensitivity" of CCDs, isn't this the same
thing as the QE of camera/chip? I don't think I have ever seen a QE for em
CCD's for various accelerating voltages/wavelenghts. Does the electron beam
directly hit the silicon photodyodes or it there an interface that converts
the incoming electrons to different (longer?) wavelengts? I know em films are
sensitive to specific acc voltages. Are CCD cameras for em the same. For long
exposures it may not matter, but for short exposures or low level intensity
does the QE of the camera come into play?

} ===== Original Message From Jenichen {Jenichen-at-proscan.de} =====
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Hank Adams
Manager
Integrated Microscopy Core
Molecular and Cellular Biology
Baylor College of Medicine
Houston, Tx 77025



From daemon Thu May 25 16:29:57 2000



From: Kim DeRuyter :      fnksd1-at-uaf.edu
Date: Thu, 25 May 2000 12:21:30 -0800
Subject: Looking for operators manual for Varian Leak Detector

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Microscopy Experts,
We have recently inherited a Varian 936-40 Porta-Test leak detector. It
came with out any documentation. Big surprise, I know. I called Varian
and they offered to sell me an operators manual for $185.00. This seems
just a bit excessive. If anyone has one, I would be happy to pay a
Xeroxing and shipping fee.
TIA
Kim DeRuyter
Electron Microscopy Technician
308 Natural Science Facility
P.O. Box 755780
University of Alaska
Fairbanks, AK 99775-5780
907-474-5452
907-474-5163 fax



From daemon Thu May 25 16:29:58 2000



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Fri, 26 May 2000 08:34:47 GMT+1200
Subject: RE: AL Peaks in EDS Spectrum

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Hi Craig

}
} I have worked on Noran Systems for 10 years. I have seen this problem several
} times and always on a JEOL SEM. Most times it was not the detector. It
} usually was a problem with alignment of the beam. The obvious point here is
} that detectors do not produce x-rays, they detect them.
}
} Regards,
}
} Craig Theberge
}

Did you ever figure out what sort of alignment problem it was, and
from exactly what the Al X-rays were being produced?

cheers

rtch


Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand



From daemon Thu May 25 16:29:58 2000



From: Kim DeRuyter :      fnksd1-at-uaf.edu
Date: Thu, 25 May 2000 13:11:54 -0800
Subject: Looking for spare parts for an ISI-40

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Looking for spare parts for an ISI-40 SEM. Particularly, column pieces,
apertures, filament assembly and wehnelt cylinders. Any suggestions would
be welcome.

Kim DeRuyter
Electron Microscopy Technician
Room 308 Natural Sciences Facility
P.O. Box 755780
University of Alaska
Fairbanks, AK 99775-5780
907-474-5452
907-474-5163 fax



From daemon Thu May 25 20:51:31 2000



From: Victor Sidorenko :      antron-at-space.ru
Date: Fri, 26 May 2000 02:15:07 +0400
Subject: Re: BSE Information

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Hi friends
I agree Steve, sometimes (I seem it depends on electron gun design and
distance between wenelt and anode, wenelt and filament) the first peak
on saturation curve is more than saturation level even in secondary
emission signal.
Victor Sidorenko, ANTRON Co.Ltd., Moscow, Russia


} ---------------------------------------------------------------------
---
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
America



From daemon Fri May 26 06:08:08 2000



From: Andrew McNaughton :      andrew.mcnaughton-at-stonebow.otago.ac.nz
Date: Fri, 26 May 2000 14:37:12 +1200
Subject: Rapid fixation of small hydrophobic molecules

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Hello

Has anyone knowledge of a method to rapidly fix adherent cell cultures so
as to prevent the loss of small hydrophobic molecules? The problem
involves subsequent diffusion of the antibody marker into the cytoplasm.
Material is examined using fluorescence/confocal microscopy The organelles
of interest in this case are mitochondia but general recommendations would
be most appreciated too.

Regards

Andrew McNaughton

______________________________________________________________________________
Andrew McNaughton
South Campus Electron Microscope Unit
C/-Department of Anatomy and Structural Biology
School of Medical Sciences
University of Otago
PO Box 913, Dunedin
NEW ZEALAND

Telephone: +64 3 479 7308
Facsimile: +64 3 479 7254

e-mail: andrew.mcnaughton-at-stonebow.otago.ac.nz
______________________________________________________________________________n




From daemon Fri May 26 06:08:09 2000



From: Corvos-at-aol.com
Date: Thu, 25 May 2000 23:40:03 EDT
Subject: Service Manuals

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All,

I would like to know if any has started an extensive collection of Microscopy
Service Manuals?

Regards,

Walter Protheroe
E-MAC, Inc.



From daemon Fri May 26 06:08:10 2000



From: Moran Scientific :      kmoran-at-goulburn.net.au
Date: Fri, 26 May 2000 14:06:02 +1000
Subject: Re: BSE Information

Contents Retrieved from Microscopy Listserver Archives
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At 05:07 PM 25-05-2000 +0100, you wrote:
Steve Chapman wrote,

} Hi
}
} Yes Luc is right I did give him an explanation.
}
} The amount of backscatter generated from a specimen depends on the kV, the
} probe size and the composition of the material under investigation. If the
} level of backscatter is insufficient under "normal" saturation conditions
} i.e. the gun is correctly saturated, then by de-saturating the larger source
} will result in a larger probe dimension on the specimen; increasing the
} probe diameter increases the volume of material involved in the production
} of BSE.
}
But how does this lead to an increase in the BSE differentiation (implying
more signal and hence less noise). Surely just defocussing would do the same
thing. Defocussing as such should not effect the BSE coeffecient, unless you
had sub surface charging or some other artefact.

Very interesting!
Ken.

Moran Scientific Pty Ltd
P.O. Box 651
Goulburn NSW 2580 Australia
Tel 02 4844 4234 (International, 61 2 48444234)
Fax 02 4844 4291 (International, 61 2 48444291)
Email {kmoran-at-goulburn.net.au}
Web Page http://www.goulburn.net.au/~kmoran/
"Patience accomplishes its object, while hurry speeds to its ruin. Gulistan
1258"



From daemon Fri May 26 06:08:11 2000



From: Jenichen :      Jenichen-at-proscan.de
Date: Fri, 26 May 2000 11:37:46 +0200
Subject: Re: new developments in imaging systems?

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hpadams schrieb:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} I have been following off and on this discussion on CCD cameras for EM.
} Much of this discussion has been concerned with "sensitivity". I am being
} naive here, but when we talk about "sensitivity" of CCDs, isn't this the same
} thing as the QE of camera/chip?

No, sensitivity means how many electrons You need for a digital response from the
CCD.

} I don't think I have ever seen a QE for em
} CCD's for various accelerating voltages/wavelenghts. Does the electron beam
} directly hit the silicon photodyodes or it there an interface that converts
} the incoming electrons to different (longer?) wavelengts?

For TEM investigations the high energy electrons (80 - 400 keV) hit a scintillator
(YAG- or Phosphor-screen). These screen emits visible photons (energy in the
region 2eV) which are detected by the CCD. If we would use the high energy
electrons directly onto the CCD the CCD would be damaged.

} I know em films are
} sensitive to specific acc voltages. Are CCD cameras for em the same.

The response for a phosphor scintillator rises linear with the energy, but reaches
saturation for high energies (higher than 200keV). This response depends also on
the material You use and on the thickness of the screen.

} For long
} exposures it may not matter, but for short exposures or low level intensity
} does the QE of the camera come into play?

If You want to get a good statistics of Your signal a response of one digital
count for one electron would be very good. If the application gives You only a
small amount of electrons to detect (Filter applications, low contrast
applications, biological application and other) the sensitivity (reponse) of the
camera should be higher to avoid long exposure times to overcome problems with
drift an sample damage. So the best cameras optimized for high sensitivity (the
screen is directly coupled with a fibreoptic to a cooled slow-scan CCD with up to
16bit digitization) reach more than one digital count per incident electron to
have a good compromise between sensitivity and good statistics.
Standard system with optical coupling do not reach this sensitivity and can be
used only for applications with high beam density.

}
}
} } ===== Original Message From Jenichen {Jenichen-at-proscan.de} =====
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} }
} }
} } Radostin Danev schrieb:
} }
} } } ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
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} } } -----------------------------------------------------------------------.
} } }
} } } Dear Sergey and others,
} } }
} } } I want to add my 2 cents as I like the topic.
} } } Most of this is a result of my experience with our TEM 2Kx2K CCD.
} } } We are working in bright field - so I cannot comment on dark field
} } } performance
} } } Several points:
} } }
} } } 1. The CCD is much more convenient - you get your pictures instantly.
} } }
} } } 2. CCD is linear and has larger dynamic range than the film.
} } }
} } } 3. The bad thing about the CCD is resolution - about 4 times lower than
} that
} } } of the film (in our camera the pixel size is 30 microns).
} }
} } If You would choose a CCD with smaller pixel size You would get a better
} } resolution.
} }
} } } So if you want to
} } } work in minimum dose you will do better with film.
} }
} } Never, a good CCD is much more sensitive than a film.
} }
} } } As I know the CCD is not
} } } performing well in terms of signal to noise at low doses (and if you have
} to
} } } work at 4 times higher magnification because of the resolution the things
} } } become much worse).
} }
} } see above , a smaller pixel gives a better sensitivity and a better
} resolution.
} }
} } }
} } }
} } } 4. The CCD has smaller observation area - again loss of information.
} }
} } use the so called Image mounting, than You get very large images with much
} more
} } image information due to the larger dynamic range and better sensitivity.
} }
} } }
} } }
} } } 5. I don't know about the detection efficiency compared to the film - it
} } } depends on the thickness of the phosphorous and the accelerating voltage.
} If
} } } a photon reaches the CCD chip it will be detected ... the problems are in
} } } the conversion electron-photon.
} }
} } The currently leading CCD systems reach single electron sensitivity at thin
} } phosphor screens and good resolution.
} }
} } } There are two sides - if you make the
} } } phosphorous thicker you will get higher detection efficiency but the point
} } } spread also increases so always a compromise is made between detection
} } } efficiency and resolution. When I say detection efficiency this is not only
} } } related to the detection of single electrons (as it detects single
} } } electrons) but more to the actual signal detected on the background of the
} } } noise. Apart from the shot noise additional noise is added due to the
} } } scintillator driven detection and thermal noise in the CCD chip.
} }
} } In good, highly sensitive CCD systems the Poisson noise of the incoming
} signal
} } is dominating, not the CCD noise.
} }
} } }
} } }
} } } The CCDs are now very popular in diffraction work because of the dynamic
} } } range and linearity.
} } }
} } } Here is one reference where a nice comparison between 2Kx2K CCD and film
} has
} } } been made:
} } }
} } } Downing, K., Hendrickson, F., Ultramicroscopy, 75, (1999), 215-233
} }
} } Thanks for that.
} }
} } }
} } }
} } } Best regards,
} } }
} } } Rado
} } }
} } } ---------------------------------------------------------------------
} } } Radostin Danev
} } } Laboratory of Ultrastructure Research
} } } National Institute for Physiological Sciences
} } } Myodaiji-cho, Okazaki 444-8585, JAPAN
} } } e-mail: rado-at-nips.ac.jp
} } } ---------------------------------------------------------------------
} }
} } --
} } Best regards / Mit freundlichen Gruessen
} } Dr. Frank Jenichen
} } Proscan elektronische Systeme GmbH
} } Tel.: +49 8195 999 -511 Fax: -512
} } mailto:Jenichen-at-proscan.de
} } ------------------------------------------------------------
} } More information concerning our products
} } and services can be found on our website
} } http://www.proscan.de
}
} Hank Adams
} Manager
} Integrated Microscopy Core
} Molecular and Cellular Biology
} Baylor College of Medicine
} Houston, Tx 77025

--
Best regards / Mit freundlichen Gruessen
Dr. Frank Jenichen
Proscan elektronische Systeme GmbH
Tel.: +49 8195 999 -511 Fax: -512
mailto:Jenichen-at-proscan.de
------------------------------------------------------------
More information concerning our products
and services can be found on our website
http://www.proscan.de




From daemon Wed May 17 21:55:49 2000



From: Schibler, Matthew :      MSchibler-at-mednet.ucla.edu
Date: Tue, 16 May 2000 12:11:18 -0700
Subject: Treated coverslips

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I suppose in principle a line-scanner type of CCD could be made to
do this, but the practical difficulties in setting it up would be
enormous - the scan system would have to be synchronously
stepping with the vertical progression of the SEM scan, and the
alignment and line geometry of the system would also have to be
exactly right. Not at all easy to achieve. Also, it is fairly well
understood that the resolution of the record screen underrepresents
the resolution of the raw signal fed to it (partly to ensure that lines
are not visible in the image).
Therefore this just seems to be the wrong approach. There are
plenty of low-cost (~10% of the cost of this camera) image
grabbers for SEM that digitise the stream of analogue data fed to
the record tube.

It would be more interesting to consider whether this type of
camera can contribute to high quality TEM imaging. I am not clear
what is limiting the resolution of current TEM digital cameras -
could a high-resolution CCD camera approach the resolution of
TEM film more closely than the current generation of these, or is
the phosphor/YAG not good enough to make it worthwhile.

Chris

Date sent: Tue, 16 May 2000 09:06:16 -0500
To: {Microscopy-at-sparc5.microscopy.com}
} From: John Foust {jfoust-at-threedee.com}


Dear List Members,

Does anyone know of a source for glass coverslips which have been treated
with something to optimize cell growth? A colleague of mine is looking for
some, particularly round ones.

Thanks.


Matthew J. Schibler Ph.D.
UCLA Brain Research Institute
1524A Gonda (Goldschmied) Center
for Neuroscience and Genetics
Los Angeles, CA 90095-1761

(310) 825-9783
FAX (310) 206-5855
E-mail: mschibler-at-mednet.ucla.edu



From daemon Wed May 17 21:56:10 2000



From: Roger Moretz :      rcmoretz-at-excite.com
Date: Tue, 16 May 2000 13:05:15 -0700 (PDT)
Subject: Re: TEM help selecting prep. equip.

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On Mon, 15 May 2000 11:19:12 -0700, Jon Krupp wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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} -----------------------------------------------------------------------.
}
}
} Dear List:
}
} A newly appointed researcher here has asked my advice on several pieces
of
} TEM spec. prep equipment. I turn to you for helpful suggestions.
}
} The research involves serial sectioning biological tissues and many
grids.
} EM is a minor, but essential component of the project. The lab runs
through
} dozens of grids and hundreds of pictures, but only 4 - 6 times a year.
Big
} bursts of activity followed by long periods of analysis and
investigations
} using other techniques. Of the hundreds of pictures taken, they may only
} use a few for data.
}
} The researcher is looking for ideas on what choices are available, how
} useful, and approximate costs of the following:
}
} Digital imaging to add to our existing JEOL 1200EX TEM - I have seen
other
} threads on this topic and would welcome any new ideas. Digital imaging
will
} save a lot of time and money since they discard so many pictuers, but the
} question of image quality is one I am to investigate.
}
} Ultra microtome - We have an older A/O Ultracut (the model before it
became
} the Reichert Ultracut E) which is OK for the sectioning we do in the
} general lab. But she wants a new one for her exclusive use. The question
is
} whether a new microtome will allow folks in her lab to do serial
} sectioning any faster or easier, or by less skilled users, than our
current
} system.
}
} Staining machine - Anyone have info on staining machines or systems for
} lots of TEM grids. I have never had to do so many grids that this was an
} issue, so I have never kept up on the offerings. If you know of something
} and/or have experience let me know. Again, this is something she would
keep
} in her lab.
}
} Tissue processing machine - Same as above for me, never did so much at
one
} time that I ever thought I needed one of these. The samples to be
processed
} are C. elegans, anything available that could do these unattended? How
} about upkeep and volumes of chemicals needed. Also an item to be kept in
} her lab.
}
} I will filter and pass on your comments. Anything you might offer will,
as
} always, be received with appreciation and thanks.
}
} Jonathan Krupp
} Microscopy & Imaging Lab
} University of California
} Santa Cruz, CA 95064
} (831) 459-2477
} jmkrupp-at-cats.ucsc.edu
}
}
}
Jon:

A few comments about the equipment. I have done literally thousands of
serial sections, so I think I can offer some first hand comments.

First: My microtome of choice for serial sectioning has been the old
Reichert OMU-3--the predecessor of the Ultracut E. I have to admit that
part of the reason was not wanting to take the time for a learning curve.
The other reason was that I found the illumination system on the old OMU-3
to be outstanding (I did repetitive serial thins and thicks over several
hundred microns to about a millimeter).

Second:Staining machine--I use/have used the LKB/Leice Ultrastainer. The
original LKB unit was a marvel--we did over 300 grids at one point, losing
only one (stuck the forceps through the formvar!!!), and no precipitates.
Can't say the same for the Leice unit, although it should be pretty much the
same. The stains are the biggest variable, as is a really rigorous cleaning
regime. Check it out.

Third: Processor--I use/love/hate the Lynx unit (currently available
through EM Sciences). I had an early unit (from Australia)--no problems
over 3 or 4 years. Have a Leica unit here--it took 4 or 5 years to get it
to work consistently, but now seems ok, and it gets substantial use in
bursts. Check out the RMC/Ventana unit--it is the progeny of the old LKB
unit, and is probably a worthy competitor. (I just can't get one for
demo--they've been promising for nearly 4 years, but I think they gave up
after last year.) Maintenance on both is fairly routine, and consumables
are not prohibitive. Both use very small volumes, and can be used osmium
through pure epoxy (if your protocol is limited to 20 or so steps).
Personally wouldn't be without one.

Hope this helps.

Roger C Moretz, Ph.D.
Dept of Toxicology
BI Pharmaceuticals

Disclaimer: I have no financial interest in any of the products or
suppliers. Just a long time user.





_______________________________________________________
Get 100% FREE Internet Access powered by Excite
Visit http://freelane.excite.com/freeisp



From daemon Wed May 17 21:56:17 2000



From: Tamara Howard :      howard-at-cshl.org
Date: Tue, 16 May 2000 16:31:45 -0400 (EDT)
Subject: Re: Treated coverslips

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Do the cells need some sort of support matrix (e.g., collagen) or are they
just not fond of glass? We do our own treating....collagen, poly-lysine,
there is a mussel protein (not a spelling error - I think it is the byssal
thread stuff from Mytilus sp.) that is sticky (CellTak?)...you can also
dissolve PS culture dishes in solvent and coat glass coverslips with the
resulting goo.

Tamara Howard
CSHL



On Tue, 16 May 2000, Schibler, Matthew wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear List Members,
}
} Does anyone know of a source for glass coverslips which have been treated
} with something to optimize cell growth? A colleague of mine is looking for
} some, particularly round ones.
}
} Thanks.
}
}
} Matthew J. Schibler Ph.D.
} UCLA Brain Research Institute
} 1524A Gonda (Goldschmied) Center
} for Neuroscience and Genetics
} Los Angeles, CA 90095-1761
}
} (310) 825-9783
} FAX (310) 206-5855
} E-mail: mschibler-at-mednet.ucla.edu
}
}
}



From daemon Wed May 17 21:56:17 2000



From: Malis, Tom :      malis-at-nrcan.gc.ca
Date: Tue, 16 May 2000 16:53:21 -0400
Subject: RE: SEM facility managers

Contents Retrieved from Microscopy Listserver Archives
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I just got off the phone with Stacie Kirsch, and discovered that I was the
3rd caller today asking about 'something in Philaldelphia'! Debby Sherman
was first and is to talk to the Meeting Arrangements person, or some such,
but I (and probably some accompanying colleagues) would be happy to
participate if something informal were organized for the Sunday afternoon.
Our Group at this Canadian federal lab:

- has 8 different beam instruments in 7 distinct 'labs' (and formally
accesses another 2 at an on-site private sector service provider),
- is manned by 18 scientists and technologists, the majority of which
are permanent,
- does a ~50-100 project/yr mix of contract work and core research
projects for and with internal programs and external clients (academics,
industrial, other federal),
- has a significant number (too many!) of internal and external
operators, and
- has a reasonable operating budget (but currently no capital), so
- we have likely encountered some variation of almost every
conceivable problem (and solved only a fraction).

If something comes to pass, now or next year, I would gladly share
experiences with others, especially as this is an aspect of delivering
science that is commonly overlooked. A couple of thoughts:

- this could easily turn into a 'gripe-athon'. Someone should be
prepared to lead it and direct it towards problem-solving if this occurs.
- Ron Anderson and I have made sporadic attempts over the years to
lead something called " Factors Influencing the Establishment of a New TEM
Facility" at numerous workshops, often with great success. This also
touched upon many of the real-world issues of an EM lab, though from the
slightly more positive viewpoint of actually some hard cash in hand.
-

Tom Malis

Group Leader - Characterization
Materials Technology Laboratory
Natural Resources Canada (Govt. of Canada)
568 Booth St., Ottawa, Canada
ph. 613-992-2310
FAX 613-992-8735
email: malis-at-nrcan.gc.ca

----------
From: Elaine Humphrey [SMTP:ech-at-unixg.ubc.ca]
Sent: May 16, 2000 11:39 AM
To: Microscopy-at-sparc5.microscopy.com
Subject: Re: SEM facility managers


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Me too, though I would like to attend the meeting in Philadelphia.
Elaine
}
}
} Collegues,
}
} In response to the current thread on the problems of facility
managers, I
} say count me in as being highly interested! If a discussion group
does get
} together at M&M it would be great to see a report posted on this
listserver
} for those of us who unfortunately can't attend the meeting. If
somebody
} could take notes and post them, I for one would be very
appreciative.
}
} Thanks!
} Dee


Dr. Elaine Humphrey
Biosciences Electron Microscopy Facility
University of British Columbia
6270 University Blvd, mail-stop Botany
Vancouver, BC
CANADA, V6T 1Z4
Phone: 604-822-3354
FAX: 604-822-6089
e-mail: ech-at-unixg.ubc.ca





From daemon Wed May 17 21:56:20 2000



From: Tom Phillips :      PhillipsT-at-missouri.edu
Date: Tue, 16 May 2000 16:23:50 -0500
Subject: Re: Treated coverslips

Contents Retrieved from Microscopy Listserver Archives
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Guess it depends on what you mean by optimize. The intestinal cell
lines i work with prefer bare glass (i wash in acetone, then ethanol,
then lots of dH2O, then boil in dH2O, then place each one on a piece
of filter paper so they aren't overlapping and then autoclave - a
pain in the neck but I think it really matters). i seed the cells in
serum containing medium without any other pre-treatement

I have coated with collagen, fibronectin, etc by placing coverslips
in 24 well trays and adding the matrix material. this makes no
difference or reduces differentiation of my cells.

many labs report cell lines that differentiate better on permeable
filters so nutrients have access to the basolateral membrane.
}
} Dear List Members,
}
} Does anyone know of a source for glass coverslips which have been treated
} with something to optimize cell growth? A colleague of mine is looking for
} some, particularly round ones.
}
} Thanks.
}
}
} Matthew J. Schibler Ph.D.
} UCLA Brain Research Institute
} 1524A Gonda (Goldschmied) Center
} for Neuroscience and Genetics
} Los Angeles, CA 90095-1761
}
} (310) 825-9783
} FAX (310) 206-5855
} E-mail: mschibler-at-mednet.ucla.edu

Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)



From daemon Wed May 17 21:56:23 2000



From: Soumitra Ghoshroy :      ghoshroy-at-nmsu.edu
Date: Tue, 16 May 2000 15:10:56 -0700
Subject: Facility Managers

Contents Retrieved from Microscopy Listserver Archives
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I very much agree with others in this issue. Unfortunately, I won't be able
to attend this year's meeting, but I will love to find out what others had
to say about managing a multi-user EM facility. Please post the highlights
of that meeting if it takes place in Philadelphia.

Thanks in advance,

Soumitra



*****************************************************************
Soumitra Ghoshroy Ph.D.
Electron Microscopy Lab
Box 3EML
New Mexico State University
Las Cruces, NM 88003
Tel: 505-646-3600
Fax: 505-646-5665
e-mail: ghoshroy-at-nmsu.edu
http://confocal.nmsu.edu/eml




From daemon Wed May 17 21:56:26 2000



From: N. Hayman :      nickh-at-u.washington.edu
Date: Tue, 16 May 2000 15:39:22 -0700 (PDT)
Subject: mounting clay-rich rocks - soils for TEM

Contents Retrieved from Microscopy Listserver Archives
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Hi all,
I believe to be on track with mounting clay rich rocks (that
disagregate readily in perturbed water (i.e. shaken or sonicated), but not
in ethylene glycol or ethanol (at rest). My goal is to mount the samples
(whole, not seperates) for analysis in TEM. I have been soaking them in
LR-White at 60C, where fixation occurs without accelerator in less than 24
hours. I am planning to (but have not yet) microtome the samples, or
potentially ion-mill.
Thoughts for the future are to use a set of glassware with a
Millipore filter and vacuum to pull ethanol(cleanser and dilator),
ethylene glycol(for swelling clay component), and an LR White "chaser" to
view "saturated" textures, as opposed to compacted.
My question is this: Can anyone point out pitfalls with this
approach that I am not seeing, again I haven't tried the whole thing yet,
but am in a position to start prepping the samples. In particular, is
microtoming preferred to ion-milling for weak, soft samples that rely on
epoxy for reinforcement? Does LR-white readily pull through a sample given
a weak vacuum and a porous plate (its viscous, but not as viscous as
water, for example)? Does swelling the clays with ethylene glycol and the
like, and then directly mounting, introduce volatiles into the column
under 120-200 kV? Any recommendations are welcome, the literature helps a
bit, but is usually sketchy about these fine details of preparation
procedure.
thanks in advance,
N

_____________________________
Nicholas W. Hayman \
Dept. of Geological Sci., U.W.\ http://www.geology.washington.edu/~hayman
Box 351310, Seattle WA 98195 \_________________________________________




From daemon Wed May 17 21:56:29 2000



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Tue, 16 May 2000 15:48:55 -0700
Subject: Re: SEM: digital 4x5 backs

Contents Retrieved from Microscopy Listserver Archives
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Dear John,
That is exactly what passive image capture systems like Quartz PCI do.
At 09:06 AM 5/16/00 -0500, you wrote:

} At 04:02 PM 5/15/00 -0700, Dr. Gary Gaugler wrote:
} } It seems to me that no digital camera system would work on a SEM
} } in place of a Polaroid or other film-based output device. Since the
} } recording CRT in a SEM is based on a sequential line scan, one
} } would need a camera that would capture each line as it is produced.
}
} What you're saying is there must be a system out there that
} digitizes that single stream of line scan intensities, then
} processes all that data inside the computer as an image as
} opposed to trying to digitize the frame buffer.
}
} - John
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Wed May 17 21:56:31 2000



From: Steve Barlow :      sbarlow-at-sunstroke.sdsu.edu
Date: Tue, 16 May 2000 16:49:51 -0700
Subject: Cryostat manufacturers

Contents Retrieved from Microscopy Listserver Archives
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Greetings all

some of my users think it is tome to retire our ancient but still
functional cryostat. Could anyone who has bought one in the recent past
give me some info about who is making them these days as well as any pros
and cons of these new-fangled models.

Thanks in advance

Steve Barlow




From daemon Wed May 17 21:56:32 2000



From: Gordon Couger :      gcouger-at-rfdata.net
Date: Tue, 16 May 2000 18:57:56 -0500
Subject: Re: (Fwd) Re: SEM: digital 4x5 backs

Contents Retrieved from Microscopy Listserver Archives
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} From: "Chris Jeffree" {cjeffree-at-srv0.bio.ed.ac.uk}
}
}
} I suppose in principle a line-scanner type of CCD could be made to
} do this, but the practical difficulties in setting it up would be
} enormous - the scan system would have to be synchronously
} stepping with the vertical progression of the SEM scan, and the
} alignment and line geometry of the system would also have to be
} exactly right. Not at all easy to achieve. Also, it is fairly well
} understood that the resolution of the record screen underrepresents
} the resolution of the raw signal fed to it (partly to ensure that lines
} are not visible in the image).
} Therefore this just seems to be the wrong approach. There are
} plenty of low-cost (~10% of the cost of this camera) image
} grabbers for SEM that digitise the stream of analogue data fed to
} the record tube.
}
} It would be more interesting to consider whether this type of
} camera can contribute to high quality TEM imaging. I am not clear
} what is limiting the resolution of current TEM digital cameras -
} could a high-resolution CCD camera approach the resolution of
} TEM film more closely than the current generation of these, or is
} the phosphor/YAG not good enough to make it worthwhile.
}

If you control the scan you only need a single light sensitive
element. You step the beam digitize the light, step the beam,
wait for the last spot to go out and repeat. You don't need
a camera. You do need a very fast responding system for
changing electron beams to light.

This system has the resolution of the scan beam. It would not
be particualy fast but you could increase the number of bits
resolution to as large a number as you wanted.

Gordon

Gordon Couger gcouger-at-couger.com

Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00




From daemon Wed May 17 21:56:34 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Tue, 16 May 2000 18:25:20 -0700
Subject: Re: (Fwd) Re: SEM: digital 4x5 backs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 10:08 AM 5/16/00, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


This is true except that the record CRT has a fixed resolution.
These are typically 2000 horizontal lines. Different scan
rates change the pixel dwell time. But the final image is still
not much more than 2000 lines. This is fine for a Polaroid
print. But if using real film, it is less than ideal or optimum.
I find that film has much more resolution than a Polaroid print.
A good alternative is the Polaroid PN (positive/negative).
But one would still have to scan the negative to get a digital
file. This is another topic, all together.


} It would be more interesting to consider whether this type of
} camera can contribute to high quality TEM imaging. I am not clear
} what is limiting the resolution of current TEM digital cameras -
} could a high-resolution CCD camera approach the resolution of
} TEM film more closely than the current generation of these, or is
} the phosphor/YAG not good enough to make it worthwhile.

Not having TEM experience, I cannot comment on this type
of application. But I am experienced with SEM usage. It would
seem to me at first blush that TEM images are continuous whereas
the SEM images are discrete. This would mean that CCD imaging
devices would work for TEM applications but not for SEM. The
common denominator remains the Polaroid and silver emulsion
film.

gg


~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Modern surfers use PC boards. You can too at
http://photoweb.net
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~



From daemon Wed May 17 21:56:44 2000



From: Michael Bode :      mb-at-Soft-Imaging.com
Date: Wed, 17 May 2000 00:36:22 -0600
Subject: Re: SEM: digital 4x5 backs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Yes, there is.

It's called a "passive" or "listening" digital image acquisition system,
such as our ADDA II or similar devices from other manufacturers.
Benefits: fairly easy to set up and use. Disadvantages (as opposed to an
"active" or "talking" system): You're still limited to what the
microscope can provide in terms of resolution, dwell time, etc.

Michael

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: John Foust [mailto:jfoust-at-threedee.com]
Sent: Tuesday, May 16, 2000 8:06 AM
To: Microscopy-at-sparc5.microscopy.com


At 04:02 PM 5/15/00 -0700, Dr. Gary Gaugler wrote:
} It seems to me that no digital camera system would work on a SEM
} in place of a Polaroid or other film-based output device. Since the
} recording CRT in a SEM is based on a sequential line scan, one
} would need a camera that would capture each line as it is produced.

What you're saying is there must be a system out there that
digitizes that single stream of line scan intensities, then
processes all that data inside the computer as an image as
opposed to trying to digitize the frame buffer.

- John



From daemon Wed May 17 21:57:12 2000



From: Debby Sherman :      sherman-at-btny.purdue.edu
Date: 17 May 2000 08:39:29 -0500
Subject: Facility manager's meeting

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Thanks to all who indicated an interest in meeting to discuss some common problems associated with managing microscopy facilities at the M&M meeting. I am in the process of arranging this and will send details once we are a bit further along.
Debby

Debby Sherman, Manager Phone: 765-494-6666
Microscopy Center in Agriculture FAX: 765-494-5896
Dept. of Botany & Plant Pathology E-mail: sherman-at-btny.purdue.edu
Purdue University
1057 Whistler Building
West Lafayette, IN 47907-1057



From daemon Wed May 17 21:57:16 2000



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Wed, 17 May 2000 08:49:21 -0500
Subject: Re: SEM: digital 4x5 backs

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Like Gary and Ken have pointed out, there are several reasons why 4x5 backs
won't work. Even if they did, there is another issue. A 4x5 back would
incorporate the photo CRT with its phosphor into the loop. That means the
electronic signal from the PMT or BSE detector has to be converted back
into an analog brightness via the photo CRT, then the digital 4x5 back
would be used to digitize the signal, and that in a most unwieldly manner.

As a rule, if you don't have to convert signals back and forth or pass them
through extra stages of processing, don't do it. I understand photo CRTs
are quite good, but there are extra focus, noise, and calibration factors
involved passing the signal through the CRT. It is far better to take the
signal straight over to digital using a good, single channel A/D converter
for the video (plus one for X and Y position rather than using the millions
of A/D converters in a CCD. Makes for a lot cheaper system, too.

Warren S.

At 04:02 PM 5/15/2000 -0700, Gary Gaugler wrote:
} At 01:38 PM 5/10/00, you wrote:
} }
} } I'm curious ... has anyone tried simply installing a digital
} } 4x5 back in place of a Polaroid 4x5 back??? For example, see:
} }
} } http://www.phaseone.com/brochures/powerfx.html
} }
} } cheerios, shAf
}
} It seems to me that no digital camera system would work on a SEM
} in place of a Polaroid or other film-based output device. Since the
} recording CRT in a SEM is based on a sequential line scan, one
} would need a camera that would capture each line as it is produced.
} Most digital backs are single or triple pass units of a single linear
} set of sensors. There are other cameras that do snapshot capture
} but even these would not work since the whole image is not present
} on the record CRT at the time of taking a picture with the digital
} camera. The final image is generated sequentially, line by line,
} on the record CRT.
}
} If the SEM image is stored in a frame buffer, the buffer can be
} converted to RS-170 TV video and frame grabbed. But the
} best that this would typically do is 640 lines.
}
} Its an interesting problem and dilemma about being in a situation
} where digital camera products simply won't work in place of
} film. But since the goal is to obtain a digital file, why not start
} with a digital interface? For example, a passive digital capture
} system would transfer the record CRT information to computer
} and directly result in a nice digital file. Alternatively, for some
} systems, an active system can be applied to directly control the
} SEM's beam. In doing so, the range of final digital image
} resolution is limited only by the attached hardware system.
}
} gary g.



From daemon Wed May 17 21:57:19 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Wed, 17 May 2000 07:07:12 -0700
Subject: Fwd: Re: SEM: digital 4x5 backs

Contents Retrieved from Microscopy Listserver Archives
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} Date: Tue, 16 May 2000 14:27:27 -0700
} To: John Foust {jfoust-at-threedee.com}
} From: "Dr. Gary Gaugler" {gary-at-gaugler.com}
} Subject: Re: SEM: digital 4x5 backs
}
} No. What I was saying was that there is not a digital
} camera system out there that will capture the line-by-line
} recording CRT output--as far as I see it at present.
}
} To get a digital file from the SEM, the method needs to
} be digital but either passively attached to the record CRT
} or actively connected as a replacement for the SEM's
} internal scan generator.
}
} gary
}
}
}
} At 07:06 AM 5/16/00, you wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Wed May 17 21:57:21 2000



From: Tyrone L. Daulton :      tdaulton-at-nrlssc.navy.mil
Date: Wed, 17 May 2000 09:37:11 -0500
Subject: TEM Job Announcement

Contents Retrieved from Microscopy Listserver Archives
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ELECTRON MICROSCOPE TECHNICIAN
Naval Research Laboratory

Announcement Number 74-0448-00
http://amp.nrl.navy.mil/code1800/74-0448.htm
Job Title: Physical Scientist, NP-1301-II, $22,563* to $49,794*
(*Includes locality pay)

Description:
The Marine Geosciences Division, Naval Research Laboratory (NRL),
Stennis Space Center, MS, seeks an electron microscope technician to
perform technical duties in support of the Marine Geosciences Electron
Microscopy Center.

The center has a state-of-the-art analytical scanning JEOL JEM-3010 TEM
equipped with a liquid/gas environmental cell, EDXS, and GIF energy
filter. The center is also equipped with a Hitachi H-600 TEM and soon
an environmental SEM. The technician will support ongoing research by
preparing specimens, analyzing samples, and assisting in general
laboratory operations. Ê

Required qualifications include a degree in physical science,
engineering, or mathematics that included 24 semester hours in physical
science and/or related engineering science such as mechanics, dynamics,
properties of materials, and electronics. The candidate must also have
one year of specialized experience equivalent to the Career Level I
(GS-Equivalent 1-4).

All candidates will be rated on the following factors: 1) Knowledge in
the preparation of transmission electron microscope (TEM) thin specimens
(preferably from fine-grained sediment/soil specimens and /or other
geological samples). 2) Knowledge in the operation of TEMâs and
scanning electron microscopes (SEMâs) and their analytical detectors.
3) Knowledge in the basic interpretation of TEM data. 4) Ability to
communicate technical concepts orally and in writing.

The Department of the Navy is an Equal Opportunity Employer.

For further information contact...

Naval Research Laboratory
Human Resources Office
455 Overlook Avenue SW
Code 1810.BMS
Washington, DC 20375-5320
(202) 767-3030

or visit the web page http://www.nrl.navy.mil/hro.htm

--
_____________________________________________________
Tyrone L. Daulton
Director: Marine Geosciences - Electron Microscopy Center
Physicist and Senior Electron Microscopist

Naval Research Laboratory
Marine Geosciences Division (Code 7400)
Stennis Space Center, MS 39529-5004

Voice (228)-688-4877
Fax (228)-688-4093
email tdaulton-at-nrlssc.navy.mil
tld-at-howdy.wustl.edu




From daemon Wed May 17 21:57:22 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Wed, 17 May 2000 07:46:42 -0700
Subject: Re: (Fwd) Re: SEM: digital 4x5 backs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 04:57 PM 5/16/00, you wrote:


} [snip]


} If you control the scan you only need a single light sensitive
} element. You step the beam digitize the light, step the beam,
} wait for the last spot to go out and repeat. You don't need
} a camera. You do need a very fast responding system for
} changing electron beams to light.
}
} This system has the resolution of the scan beam. It would not
} be particualy fast but you could increase the number of bits
} resolution to as large a number as you wanted.
}
} Gordon
}
} Gordon Couger gcouger-at-couger.com
}
} Stillwater, OK www.couger.com/gcouger
} 405 624-2855 GMT -6:00

The light sensitive element is already in-place. It is the
Everhart-Thornley scintillator detector, or any other
SE or BSE detector on a SEM. Just passively tap into
the record CRT signals, digitize the detected video
(SE/BSE), and save the resulting image as a digital
file. Changing scan rates will change pixel dwell time.
But the final image ought to still be a digital representation
of what was intended to go to the film/Polaroid camera.

Again, the other option is active control of the SEM's beam.
This just swaps the internal scan generator with an external
one. The video system and digitization is the same as
for a passive system. A robust active system will be capable
of producing higher resolution digital images than a passive
system. This is because a good active system can go up
to 4096 horizontal pixels (12-bit D/A converter) and the
pixel dwell time is usually adjustable. The only down side
is the total frame time based on pixel dimensions and
dwell time. At extremes, one could be waiting a very long
time for a single image. If the beam is not stable, and
as pointed out in an earlier posting, the drive circuits
are not stable, there would be some upper limit on pixel
dimensions and dwell time. Beyond this limit, the image
would appear to shift as the beam shifted during the
capture.

Several of the current generation SEMs have incorporated
digital control and digital image capture. But these SEMs
are of course at today's prices. Its rather easy to breathe
new life into an older SEM by adding active or passive
third party digital control/capture systems. One gets
essentially a "new" modern SEM at a fraction of the cost
of buying a new one.

gary g.



From daemon Wed May 17 21:57:33 2000



From: Diane Curley :      Diane.Curley-at-unilever.com
Date: Wed, 17 May 2000 10:51:35 -0500
Subject: Freeze Fracture System

Contents Retrieved from Microscopy Listserver Archives
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We are exploring the possibility of purchasing a freeze fracture system to do
FFTEM of emulsions/microemulsions. Does anyone have any recommendations? Does
anyone have a used system available?

Thank you,
Diane



From daemon Wed May 17 21:57:31 2000



From: Gordon Vrololjak :      gvrdolja-at-nature.Berkeley.EDU
Date: Wed, 17 May 2000 08:56:24 -0700 (PDT)
Subject: Re: mounting clay-rich rocks - soils for TEM

Contents Retrieved from Microscopy Listserver Archives
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Hello Nicholas,
I've looked at several soils similar to your easily disaggregated clay
rich rocks in the TEM and find that the best procedure
is to imbed aggregates in 2% agar to hold the aggregates together. The
aggregates are then cut out as agar-soil cubes and chemical processing
(fixation, Os-fix, buffer rinse, dehydration, resin-solvent washes, resin
infiltration) done directly onto the agar cubes. The cubes are placed
into molds and after curing, microtomed to 40-60nm sections. The sections
come out nicely, but if you have a high primary mineral content (quartz,
feldspars } 15%) then you'll have a lot of torn sections. Use of a
diamond knife rather than glass gives you significantly better results.

My methods used in my thesis are on the web at:
http://wilfred.berkeley.edu/~gordon/PHD
Look particularly at chapter four which concentrates on TEM methods and
results. If it would be of use, I'll be glad to send a cdrom copy of my
thesis to you.

\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
Gordon Ante Vrdoljak Electron Microscope Lab
ICQ 23243541 http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
gvrdolja-at-nature.berkeley.edu UC Berkeley
phone (510) 642-2085 Berkeley CA 94720-3330
fax (510) 643-6207 cell (510) 290-6793

On Tue, 16 May 2000, N. Hayman wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi all,
} I believe to be on track with mounting clay rich rocks (that
} disagregate readily in perturbed water (i.e. shaken or sonicated), but not
} in ethylene glycol or ethanol (at rest). My goal is to mount the samples
} (whole, not seperates) for analysis in TEM. I have been soaking them in
} LR-White at 60C, where fixation occurs without accelerator in less than 24
} hours. I am planning to (but have not yet) microtome the samples, or
} potentially ion-mill.
} Thoughts for the future are to use a set of glassware with a
} Millipore filter and vacuum to pull ethanol(cleanser and dilator),
} ethylene glycol(for swelling clay component), and an LR White "chaser" to
} view "saturated" textures, as opposed to compacted.
} My question is this: Can anyone point out pitfalls with this
} approach that I am not seeing, again I haven't tried the whole thing yet,
} but am in a position to start prepping the samples. In particular, is
} microtoming preferred to ion-milling for weak, soft samples that rely on
} epoxy for reinforcement? Does LR-white readily pull through a sample given
} a weak vacuum and a porous plate (its viscous, but not as viscous as
} water, for example)? Does swelling the clays with ethylene glycol and the
} like, and then directly mounting, introduce volatiles into the column
} under 120-200 kV? Any recommendations are welcome, the literature helps a
} bit, but is usually sketchy about these fine details of preparation
} procedure.
} thanks in advance,
} N
}
} _____________________________
} Nicholas W. Hayman \
} Dept. of Geological Sci., U.W.\ http://www.geology.washington.edu/~hayman
} Box 351310, Seattle WA 98195 \_________________________________________
}
}
}
}



From daemon Wed May 17 21:57:36 2000



From: Martin J. Roe :      m.roe-at-mluri.sari.ac.uk
Date: Wed, 17 May 2000 18:10:30 +0000
Subject: EDS mineral identification and database

Contents Retrieved from Microscopy Listserver Archives
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Dear All,
Does anyone know of an EDS mineral spectrum database or
mineral identification program? Is there such a thing available?
Possibly a program that would allow the input of an image file of
the unknown mineral spectrum thereby generating a list of possible
'best-fit' candidates. Or maybe it would work by predicting what the
spectrum of a certain mineral should look like for a particular beam
voltage etc.
Regards
Martin Roe


Martin J. Roe
Macaulay Land Use Research Institute
Craigiebuckler
Aberdeen
Scotland
UK
Phone 01224 318611
e-mail m.roe-at-mluri.sari.ac.uk



From daemon Wed May 17 21:57:42 2000



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Wed, 17 May 2000 12:06:17 -0700
Subject: RE: new developments in imaging systems?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thank you Mickhael.

I will wait for info about sensitivity. I think, for EM in particular,
sensitivity is very important parameter of the system. I am surprised that
manufacturers don't have data on this matter. If sensitivity, say 10 times
higher than SO-163 film - it may be a great reason to switch from film to
the CCD. The major disadvantage of the CCD cameras for TEM is their price
in my point of view.
Thanks for your respond.

Sergey


} Date: Wed, 17 May 2000 11:34:05 -0600
} From: Michael Bode {mb-at-Soft-Imaging.com}
} Subject: RE: new developments in imaging systems?
} To: 'Sergey Ryazantsev' {sryazant-at-ucla.edu}
} X-Mailer: Internet Mail Service (5.0.1457.3)
}
} Sergey,
}
} I will try to find out what we have regarding the relative
} sensitivities. One thing that I can say now is, that we acquire images
} with a 50 or 100 msec exposure of the digital camera when the film
} requires an exposure of about 2 seconds. This would mean a 20 to 40
} times better sensitivity of the CCD camera. But to answer your question
} in more detail would require to compare also the resolution of film and
} camera and that is where it becomes very difficult, as it is not easy to
} determine the resolution of film in terms of spatial resolution and
} dynamic range, as both are interwoven. I will try to find some answers
} for you.
}
} Regarding the linearity: As the CCDs simply count Photons, they have
} almost perfect linearity over their complete dynamic range. Even more so
} for TEMs, where all Photons have the same energy and the quantum
} efficiency does not change from photon to photon. The Phosphor is a part
} of a system that could theoretically introduce some non-linearities.
} However, I did measure the linearity of some TEM camera systems a few
} years back and did not find any significant deviations from linearity.
}
} Michael
}
} Michael Bode, Ph.D.
} Soft Imaging System Corp.
} 1675 Carr St., #105N
} Lakewood, CO 80215
} ===================================
} phone: (888) FIND SIS
} (303) 234-9270
} fax: (303) 234-9271
} email: mailto:info-at-soft-imaging.com
} web: http://www.soft-imaging.com
} ===================================
}
}
}
} -----Original Message-----
} From: Sergey Ryazantsev [mailto:sryazant-at-ucla.edu]
} Sent: Wednesday, May 17, 2000 1:29 AM
} To: Michael Bode
} Subject: RE: new developments in imaging systems?
}
}
} Mickhael hello
}
} I have question for you. I am thinking about adding CCD camera to my
} JEM1200EX. The information I gathered from Internet is not so
} optimistic.
} The standard CCD resolution is about 1-1.5 million pixels, 2 million is
} a
} max as I understand. The price for cheaper camera is about 20-30 K$ -
} much
} more that I expect to spend for "film" process. There are two things
} may
} attract me to the modern CCD camera: dynamic range and sensitivity. I
} am
} pretty sure that dynamic range for CCD itself is a few orders better
} than
} any film available. But what about phosphorus screen? Does it reduce
} dynamic range for the EM images? How dramatic? This is my first
} question.
} The next question is: could you tell me something about sensitivity of
} the
} modern CCD cameras used in EM? I am using dark field imaging at x80K
} magnification and the exposure time for SO-163 (non deluded D-19, 7 min,
} 20oC) is about 2 sec. I called GATAN, but they did not say anything
} useful.
} Could you provide some comparison of your side-mount camera with
} sensitivity of the SO-163 film at condition I mentioned? I will greatly
} appreciate any information in this matter. Thanks. Sergey.
}
}
} } Date: Fri, 12 May 2000 13:46:01 -0600
} } From: Michael Bode {mb-at-Soft-Imaging.com}
} } Subject: RE: new developments in imaging systems?
} } To: "'Microscopy-at-MSA.Microscopy.Com'"
} {Microscopy-at-sparc5.microscopy.com}
} } Cc: 'Margaret Dienelt' {brannign-at-asrr.arsusda.gov}
} } X-Mailer: Internet Mail Service (5.0.1457.3)
} }
} } -----------------------------------------------------------------------
} -
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
}

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu
http://www.bol.ucla.edu/~sryazant




From daemon Wed May 17 21:57:48 2000



From: Michelle.Taurino-at-aventis.com
Date: Wed, 17 May 2000 14:33:22 -0500
Subject: Re-embedding paraffin sections in plastic

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Any suggestions on re-embedding paraffin sections in epon?
Has anyone worked with the "pop out" method using BEEM capsules?

Any suggestions would be helpful...

Michelle Taurino
Aventis Pharmaceuticals
Bioimaging and Molecular Histology
Michelle.Taurino-at-aventis.com
908-231-3357



From daemon Wed May 17 21:58:07 2000



From: Huggins, Bradley J :      HUGGINBJ-at-bp.com
Date: Wed, 17 May 2000 15:44:12 -0500
Subject: RE: SEM: digital 4x5 backs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I'm no digital imaging guru, but I have been following this thread with
interest. This whole discussion has been of interest to me because of the
idea of the passive 4x5 (camera back) "detector" that could provide an
extremely simple user venue for converting those countless analog SEMs into
a very usable digital output format.

We have a 10 year old JEOL 840 microanalysis system that we would like to
see in operation for another 10 years, and I think it may be possible.
However, digital imaging, although "doable", is not "convenient" for us, at
this point for this instrument. Too much time and effort is required in
digital acquisition, maintenance of instrument condition info with the
image, labeling the image, and archiving. Then on top of it all, the
digital image is often not quite as good as the analog Polaroid shot that
also has the Mag, Bar Scale, KeV, WD, and other info permanently
incorporated (We use Type 53 for much of our work.) Don't get me wrong, we
do some digital imaging with it, but "it ain't the same" as using a newer
digital scope with integrated digital control and digital interface. If
someone put such a passive digital detector into a 4x5 camera back-type
mount, and it was capable of passively detecting at least 2500 horizontal
lines and an approximately equivalent vertical resolution, I believe it
would be a huge success with the analog SEMs. The 840 and many other
instruments like it are great conventional SEMs, and such a simple interface
(if affordable) would greatly extend their value. Many of these scanners
have exceptional imaging capability, but often the easiest/best way to
record that impressive image is by Polaroid film.

Is it doable?
Brad Huggins

} ----------
} From: Michael Bode[SMTP:mb-at-Soft-Imaging.com]
} Sent: Wednesday, May 17, 2000 1:36 AM
} To: 'Microscopy-at-MSA.Microscopy.Com'
} Cc: 'John Foust'
} Subject: RE: SEM: digital 4x5 backs
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Yes, there is.
}
} It's called a "passive" or "listening" digital image acquisition system,
} such as our ADDA II or similar devices from other manufacturers.
} Benefits: fairly easy to set up and use. Disadvantages (as opposed to an
} "active" or "talking" system): You're still limited to what the
} microscope can provide in terms of resolution, dwell time, etc.
}
} Michael
}
} Michael Bode, Ph.D.
} Soft Imaging System Corp.
} 1675 Carr St., #105N
} Lakewood, CO 80215
} ===================================
} phone: (888) FIND SIS
} (303) 234-9270
} fax: (303) 234-9271
} email: mailto:info-at-soft-imaging.com
} web: http://www.soft-imaging.com
} ===================================
}
}
}
} -----Original Message-----
} } From: John Foust [mailto:jfoust-at-threedee.com]
} Sent: Tuesday, May 16, 2000 8:06 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Re: SEM: digital 4x5 backs
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} At 04:02 PM 5/15/00 -0700, Dr. Gary Gaugler wrote:
} } It seems to me that no digital camera system would work on a SEM
} } in place of a Polaroid or other film-based output device. Since the
} } recording CRT in a SEM is based on a sequential line scan, one
} } would need a camera that would capture each line as it is produced.
}
} What you're saying is there must be a system out there that
} digitizes that single stream of line scan intensities, then
} processes all that data inside the computer as an image as
} opposed to trying to digitize the frame buffer.
}
} - John
}
}



From daemon Wed May 17 21:58:29 2000



From: Mark Blackford :      mgb-at-ansto.gov.au
Date: Thu, 18 May 2000 09:13:57 +1100
Subject: Re: EDS mineral identification and database

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} Dear All,
} Does anyone know of an EDS mineral spectrum database or
} mineral identification program? Is there such a thing available?
} Possibly a program that would allow the input of an image file of
} the unknown mineral spectrum thereby generating a list of possible
} 'best-fit' candidates. Or maybe it would work by predicting what the
} spectrum of a certain mineral should look like for a particular beam
} voltage etc.
} Regards

} Martin Roe

Martin,

I know of no publicly available database of mineral EDX spectra or
identification software. However, I believe several commercial EDX systems
have a facility for generating your own database by collecting spectra from
standard mineral samples. The software can then compare a spectrum from an
unknown with those in the database.

One must be very careful of comparing like with like. All instrument
parameters such as kV, tilt, etc, etc must be equivalent for a reasonable
chance of a correct match.

I use the Desk Top Spectrum Analyser (DTSA) program from NIST to simulate
EDX spectra from minerals, ceramics etc from the known composition. It
requires a fair bit of effort to master the program but I feel it is worth
the effort. Of course it does a lot more than just simulate spectra,
including analysis of real measured spectra with a huge amount of
flexibility in choosing analysis parameters.

DTSA is available free from the NIST web site:

http://www.cstl.nist.gov/div837/837.02/dtsa.html

Hope this helps,






Mark Blackford
TEM Group
Materials Division, ANSTO
PMB 1,
Menai, N.S.W.
Australia
2234

Phone 61 2 9717 3027
Fax 61 2 9543 7179

Disclaimer:
The views expressed in this E-mail message do not necessarily represent the
official views of ANSTO from which this message was conveyed.




From daemon Wed May 17 21:58:58 2000



From: Rosemary Walsh :      rw9-at-psu.edu
Date: Wed, 17 May 2000 21:13:23 -0400
Subject: Re: Facility manager's meeting

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Debby,
Please include me in any meetings planned to discuss EM lab management.
Rosemary



From daemon Wed May 17 21:58:59 2000



From: David Henriks :      Henriks-at-CompuServe.COM
Date: Wed, 17 May 2000 22:02:51 -0400
Subject: RE: SEM: digital 4x5 backs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Brad:

For what it's worth - if anything, there is a company out here in
California called Silicon Film Technologies that has developed a technology
that converts a traditional 35mm SLR camera into a digital camera. You
essentially just replace the film with something they call (e)film. I
don't know if they have any interest in developing something for this
application, but it may be worth a look. It's a pretty nifty concept for
people who want to do both traditional film photography and also digital
photography with the same equipment.

I have copied a blurb from their website to give you an overview of what
they do:

"Our EFS product suite is a digital photo system comprised of an electronic
film cartridge and a carrier/adapter. The user simply inserts the
cartridge, called (e)film, into the film cavity in the back of a
conventional 35mm SLR camera. After recording up to 24 images, the
cartridge is placed into a carrier/adapter, called (e)port, which may then
be connected directly to a personal computer, enabling images to be
downloaded quickly into the computer and then printed, sent via email, or
modified into a photo end product using photo management software such as
Adobe PhotoShop LE, which comes bundled with the EFS system. We will also
market a digital photo storage module, called (e)box, which can store and
transport hundreds of digital images in the field
when the photographer does not have access to a computer.

The EFS system transforms conventional camera equipment into a digital
image capture system. It is the only system currently available that
provides the convenience and flexibility of choosing between conventional
and electronic film formats
with the same camera body. The product is aimed toward the large, installed
base of 35mm camera owners who would like to enjoy the benefits of digital
imaging while not giving up the cameras, lenses, and photo accessories with
which they are
familiar."

You can check out their website for more information at
www.siliconfilm.com.

DISCLAIMER: I do own a small amount of stock in their parent company,
Irvine Sensors Corporation. Of course, even if all of you bought a dozen
of their neat little cameras, I'd still have to keep my day job, but I
thought I should disclose it.

Best regards-

David
Writing at 4:13:48 PM on 05/17/2000

***************************************************************************
************************

David Henriks
Vice President TEL: 800-728-2233
(toll free in the USA)
South Bay Technology, Inc. +1-949-492-2600
1120 Via Callejon FAX: +1-949-492-1499
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

***************************************************************************
************************

} } } } } Please visit us at http://www.southbaytech.com { { { { {

Manufacturers of precision sample preparation equipment and supplies for
metallography, crystallography and electron microscopy.

Message text written by "Huggins, Bradley J"
}
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


I'm no digital imaging guru, but I have been following this thread with
interest. This whole discussion has been of interest to me because of the
idea of the passive 4x5 (camera back) "detector" that could provide an
extremely simple user venue for converting those countless analog SEMs into
a very usable digital output format.

We have a 10 year old JEOL 840 microanalysis system that we would like to
see in operation for another 10 years, and I think it may be possible.
However, digital imaging, although "doable", is not "convenient" for us, at
this point for this instrument. Too much time and effort is required in
digital acquisition, maintenance of instrument condition info with the
image, labeling the image, and archiving. Then on top of it all, the
digital image is often not quite as good as the analog Polaroid shot that
also has the Mag, Bar Scale, KeV, WD, and other info permanently
incorporated (We use Type 53 for much of our work.) Don't get me wrong,
we
do some digital imaging with it, but "it ain't the same" as using a newer
digital scope with integrated digital control and digital interface. If
someone put such a passive digital detector into a 4x5 camera back-type
mount, and it was capable of passively detecting at least 2500 horizontal
lines and an approximately equivalent vertical resolution, I believe it
would be a huge success with the analog SEMs. The 840 and many other
instruments like it are great conventional SEMs, and such a simple
interface
(if affordable) would greatly extend their value. Many of these scanners
have exceptional imaging capability, but often the easiest/best way to
record that impressive image is by Polaroid film.

Is it doable?
Brad Huggins {



From daemon Wed May 17 21:59:02 2000



From: jim :      jim-at-proscitech.com.au
Date: Thu, 18 May 2000 14:15:02 +1000
Subject: RE: new developments in imaging systems?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Martin:
An interesting, but difficult idea. Besides the problem of instrument
parameters, and settings, there is the problem of mineral compositions being
in many cases very similar, but having different structures. For example,
there are several iron oxide/hydroxide phases that would be difficult to
tell apart with an EDS analysis. More complex are the silicates which have a
number of different major structural families, many having the same or
similar chemical compositions. There are sites on the web that would allow
you to input an element list and it will output all the minerals with those
elements and their formulas. It won't identify the minerals, but it would
give you a start. To identify them you need to do optical or x-ray
diffraction or some other more appropriate technique.
Michael L. Boucher Sr.
mboucher-at-isd.net
http://www.isd.net/mboucher
-----Original Message-----
} From: Martin J. Roe {m.roe-at-mluri.sari.ac.uk}
To: microscopy-at-sparc5.microscopy.com {microscopy-at-sparc5.microscopy.com}


If sensitivity was ten times higher, one would in some cases save some beam
damage and in most cases suffer excessive electron noise.
As a rough guide, electron noise becomes apparent at 30x enlargement, rarely a
problem. Magnification is a linear function and electron density relates to an
area, but clearly very short exposure images would be much noisier and could
not be enlarged nearly as much. When not enough electrons form an image it
appears grainy, which makes it unsuitable for further enlarging. Photo
enlarging utilises higher depths-of-field and without this, very high power TEM
is much, much harder.
By nature, slower emulsions have finer grain and higher resolution and
contrast. It is fortuitous that these desirable characteristics run in tandem
with relatively long exposures, so the image is formed by more electrons.
TEM films rate about 6 ISO, so X-ray film at about ISI 3000 is about a 10x
faster than TEM films. Unfortunately its rather grainy, but if the exposure was
well adjusted, chances are that electron noise would be more bothersome than
the film's grain.
Digital is inevitable and already fairly common, but some aces remain with
conventional film.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Thursday, May 18, 2000 5:06 AM, Sergey Ryazantsev [SMTP:sryazant-at-ucla.edu]
wrote:
}
}
} Thank you Mickhael.
}
} I will wait for info about sensitivity. I think, for EM in particular,
} sensitivity is very important parameter of the system. I am surprised that
} manufacturers don't have data on this matter. If sensitivity, say 10 times
} higher than SO-163 film - it may be a great reason to switch from film to
} the CCD. The major disadvantage of the CCD cameras for TEM is their price
} in my point of view.
} Thanks for your respond.
}
} Sergey
}
}
} } Date: Wed, 17 May 2000 11:34:05 -0600
} } From: Michael Bode {mb-at-Soft-Imaging.com}
} } Subject: RE: new developments in imaging systems?
} } To: 'Sergey Ryazantsev' {sryazant-at-ucla.edu}
} } X-Mailer: Internet Mail Service (5.0.1457.3)
} }
} } Sergey,
} }
} } I will try to find out what we have regarding the relative
} } sensitivities. One thing that I can say now is, that we acquire images
} } with a 50 or 100 msec exposure of the digital camera when the film
} } requires an exposure of about 2 seconds. This would mean a 20 to 40
} } times better sensitivity of the CCD camera. But to answer your question
} } in more detail would require to compare also the resolution of film and
} } camera and that is where it becomes very difficult, as it is not easy to
} } determine the resolution of film in terms of spatial resolution and
} } dynamic range, as both are interwoven. I will try to find some answers
} } for you.
} }
} } Regarding the linearity: As the CCDs simply count Photons, they have
} } almost perfect linearity over their complete dynamic range. Even more so
} } for TEMs, where all Photons have the same energy and the quantum
} } efficiency does not change from photon to photon. The Phosphor is a part
} } of a system that could theoretically introduce some non-linearities.
} } However, I did measure the linearity of some TEM camera systems a few
} } years back and did not find any significant deviations from linearity.
} }
} } Michael
} }
} } Michael Bode, Ph.D.
} } Soft Imaging System Corp.
} } 1675 Carr St., #105N
} } Lakewood, CO 80215
} } ===================================
} } phone: (888) FIND SIS
} } (303) 234-9270
} } fax: (303) 234-9271
} } email: mailto:info-at-soft-imaging.com
} } web: http://www.soft-imaging.com
} } ===================================
} }
} }
} }
} } -----Original Message-----
} } From: Sergey Ryazantsev [mailto:sryazant-at-ucla.edu]
} } Sent: Wednesday, May 17, 2000 1:29 AM
} } To: Michael Bode
} } Subject: RE: new developments in imaging systems?
} }
} }
} } Mickhael hello
} }
} } I have question for you. I am thinking about adding CCD camera to my
} } JEM1200EX. The information I gathered from Internet is not so
} } optimistic.
} } The standard CCD resolution is about 1-1.5 million pixels, 2 million is
} } a
} } max as I understand. The price for cheaper camera is about 20-30 K$ -
} } much
} } more that I expect to spend for "film" process. There are two things
} } may
} } attract me to the modern CCD camera: dynamic range and sensitivity. I
} } am
} } pretty sure that dynamic range for CCD itself is a few orders better
} } than
} } any film available. But what about phosphorus screen? Does it reduce
} } dynamic range for the EM images? How dramatic? This is my first
} } question.
} } The next question is: could you tell me something about sensitivity of
} } the
} } modern CCD cameras used in EM? I am using dark field imaging at x80K
} } magnification and the exposure time for SO-163 (non deluded D-19, 7 min,
} } 20oC) is about 2 sec. I called GATAN, but they did not say anything
} } useful.
} } Could you provide some comparison of your side-mount camera with
} } sensitivity of the SO-163 film at condition I mentioned? I will greatly
} } appreciate any information in this matter. Thanks. Sergey.
} }
} }
} } } Date: Fri, 12 May 2000 13:46:01 -0600
} } } From: Michael Bode {mb-at-Soft-Imaging.com}
} } } Subject: RE: new developments in imaging systems?
} } } To: "'Microscopy-at-MSA.Microscopy.Com'"
} } {Microscopy-at-sparc5.microscopy.com}
} } } Cc: 'Margaret Dienelt' {brannign-at-asrr.arsusda.gov}
} } } X-Mailer: Internet Mail Service (5.0.1457.3)
} } }
} } } -----------------------------------------------------------------------
} } -
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} }
} } } To Subscribe/Unsubscribe -- Send Email to
} } ListServer-at-MSA.Microscopy.Com
} } } On-Line Help
} } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } -----------------------------------------------------------------------
} } .
} } }
} } }
} } } Margaret,
} } }
} } } As a former user and current vendor of such systems as you are
} } inquiring
} } } about I can try to provide a bit of information regarding camera
} } } improvements:
} } }
} } } There have been a number of improvements, but I am not sure what you
} } are
} } } comparing the latest cameras against. Cameras are now usually cooled
} } and
} } } provide 12 bits per pixel, the number of pixels has gone up a bit (but
} } } not much in general), and cameras read out faster than they used to (up
} } } to 20 fps and more). I think all cameras now use a line transfer
} } } mechanism, which makes shutters obsolete.
} } } On the software side, real-time FFT and real-time shading correction
} } can
} } } be done now due to faster computers without special processing boards,
} } } and there have been other software developments that make using the
} } } cameras and computers easier.
} } } Other changes that affect the usability of cameras is the use of
} } } pneumatics to insert and retract the phosphors, higher frame rates for
} } } live viewing with the camera, etc.
} } }
} } } If you have questions, please give me a call, drop me an email, or go
} } to
} } } our web site.
} } }
} } } Michael
} } }
} } }
} } } Michael Bode, Ph.D.
} } } Soft Imaging System Corp.
} } } 1675 Carr St., #105N
} } } Lakewood, CO 80215
} } } ===================================
} } } phone: (888) FIND SIS
} } } (303) 234-9270
} } } fax: (303) 234-9271
} } } email: mailto:info-at-soft-imaging.com
} } } web: http://www.soft-imaging.com
} } } ===================================
} } }
} } }
} } }
} } } -----Original Message-----
} } } } From: Margaret Dienelt [mailto:brannign-at-asrr.arsusda.gov]
} } } Sent: Thursday, May 11, 2000 12:43 PM
} } } To: Microscopy-at-sparc5.microscopy.com
} } } Subject: TEM: new developments in imaging systems?
} } }
} } }
} } } -----------------------------------------------------------------------
} } -
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} }
} } } To Subscribe/Unsubscribe -- Send Email to
} } ListServer-at-MSA.Microscopy.Com
} } } On-Line Help
} } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } -----------------------------------------------------------------------
} } .
} } }
} } }
} } } Hi,
} } }
} } } Year after year I hopefully gather information about digital imaging
} } } systems for TEMs (ours is a JEOL 100CX) , only to learn we have no
} } } money. This year it looks like it might really happen but I have not
} } } kept up with innovations in the field and am wondering the following:
} } }
} } } 1. Anything new in the last two years -- especially in terms of
} } } cameras? I'm most familiar with the Gatan and AMT systems but their
} } } web sites don't reflect much in the way of changes over a year ago.
} } } 2. With more and more microscopists finally getting their systems --
} } } I'd love to get feedback.
} } }
} } } Thanks,
} } } Margaret
} } }
} } } P.S. Would welcome contacts from vendors.
} } }
} } } --
} } } Margaret Dienelt
} } }
} } } Plant Pathologist
} } } Electron Microscopy Lab
} } }
} } } Floral and Nursery Plants Research Unit
} } } U.S. National Arboretum/Agricultural Research Service/USDA
} } }
} } } B. 010A, Rm. 238, BARC-W
} } } 10300 Baltimore Avenue
} } } Beltsville MD. 20705 USA
} } }
} } } (301) 504-6097
} } } Fax: (301) 504-5096
} } }
} } }
} } _____________________________________
} }
} } Sergey Ryazantsev Ph. D.
} } Electron Microscopy
} } UCLA School of Medicine
} } Department of Biological Chemistry
} } Box 951737
} } Los Angeles, CA 90095-1737
} }
} } Phone: (310) 825-1144
} } Pager: (310) 845-0248
} } FAX (departmental): (310) 206-5272
} } mailto:sryazant-at-ucla.edu
} } http://www.bol.ucla.edu/~sryazant
} }
} }
} _____________________________________
}
} Sergey Ryazantsev Ph. D.
} Electron Microscopy
} UCLA School of Medicine
} Department of Biological Chemistry
} Box 951737
} Los Angeles, CA 90095-1737
}
} Phone: (310) 825-1144
} Pager: (310) 845-0248
} FAX (departmental): (310) 206-5272
} mailto:sryazant-at-ucla.edu
} http://www.bol.ucla.edu/~sryazant
}
}



From daemon Thu May 18 23:02:14 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Wed, 17 May 2000 22:58:32 -0700
Subject: RE: SEM: digital 4x5 backs

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I can identify with your situation. While I do not know the intricacies
of the JEOL instruments, some SEMs are made to accept external
drive for x-ray analysis. This same input scheme works perfectly
for active control of the SEM and direct digital capture of images.
At the worst, you can replicate the resolution of your record CRT
using a passive mode. How easy either of these modes are depends
greatly on how the SEM system was designed.

gary g.

At 01:44 PM 5/17/00, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Thu May 18 23:02:27 2000



From: ard-at-ansto.gov.au (Arthur Day)
Date: Thu, 18 May 2000 18:28:19 +1000
Subject: Re: EDS mineral identification and database

Contents Retrieved from Microscopy Listserver Archives
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} Dear Martin:
} An interesting, but difficult idea. Besides the problem of instrument
} parameters, and settings, there is the problem of mineral compositions being
} in many cases very similar, but having different structures.

And then there is also the converse: the problem of mineral compositions
being in many cases very different, but having the same structures. One of
a huge number of examples would be the feldspar series where Na and Ca can
substitute for each other continuously from Na to Ca endmembers.

Various other bits of crystallographic information, some intangibles such
as crystal shape and growth habit, associations with other minerals and so
on, would have to be taken into account in such software for it to really
zero in on an unambiguous ID for you.



Arthur Day, Electron Microscope Unit Phone: 61-2-9717-3457
Ansto Materials Division Fax: 61-2-9543-7179
PMB 1, Menai (Sydney), NSW, 2234 Email: ard-at-ansto.gov.au
Australia www: http://www.ansto.gov.au/




From daemon Thu May 18 23:02:52 2000



From: LAB. DE MICROSCOPIA ELECTRONICA - FI - UNER :      microsc-at-fi.uner.edu.ar
Date: Thu, 18 May 2000 10:55:01 +0000
Subject: looking for ETEC Omniscan manuals

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Hello all .....
we have an old ETEC Omniscan SEM, and we need to contact with any
people that can supply manuals ....( fundamentally electric and electronic
schematic )

any help is welcome

best regards


===================================================
Fernando D. Balducci
Laboratorio de Microscopia Electr—nica
Facultad de Ingenier’a - Bioingenieria
Universidad Nacional de Entre Rios
Argentina
e-mail: microsc-at-fi.uner.edu.ar
tel: 54 43 975100
fax: 54 43 975077


===================================================



From daemon Thu May 18 23:03:00 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Thu, 18 May 2000 06:27:27 -0700
Subject: RE: SEM: digital 4x5 backs

Contents Retrieved from Microscopy Listserver Archives
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At 07:02 PM 5/17/00, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

I think that their aim is a bit off the target. Its nice to not have to
give up the hardware but their offering makes the user give up the
benefits of 35mm film--frame size and selection of speed. And,
a field of view factor of 2.8 is absurd.

I beta tested what must have been an initial offering of this
product early last year. It was by a different company. Same idea
though. Same deficiencies. The sensor is 1.3M pixels and
has an odd aspect ratio of 1.25 (35mm frame is 1.5). Also,
and most importantly, the product does not image the entire
35mm frame, only a small central portion. 1.3M pixel
point and shoot cameras are a lot cheaper than this
silicon film thing. A really dedicated user would have to buy
more than one silicon film insert. At $600 each, that would
buy a lot of P&S cameras. But there is no need to do that
since one just pops out a SmartMedia or Compact Flash
module ($150 or so each).

I still say wait and see how the Fuji Finepix S1 Pro performs.
If it lives up to spec, I'd say that it will be a raging success
and a major turning point in digital cameras which are
based on the 35mm format.

gary g.


~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Modern surfers use PC boards. You can too at
http://photoweb.net
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~



From daemon Thu May 18 23:02:46 2000



From: ComCryLab1-at-aol.com
Date: Thu, 18 May 2000 09:36:31 EDT
Subject: TEM: Help

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Dear Friends,
Can anyone give me advice regarding the dismantling and
the packing for shipment of a Philips 201 TEM? I need to
pay particular attention to not disturbing the alignment.
I plan to move the TEM from Univ. of Delaware to Naples, Florida
in an enclosed U-Haul Trailer. Any help or suggestions
will be most welcome.
If anyone has hands-on experience in doing this sort of thing
and is willing to give me a hand at U-Del, please contact me
and name your price. I'll gladly pay for the assistance.
Best regards,
Mike Urbanik
www.crystalguru.com



From daemon Thu May 18 23:03:03 2000



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Thu, 18 May 2000 08:42:49 -0500
Subject: RE: SEM: digital 4x5 backs

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I will reiterate my point and that of others. An SEM technically has video
only coming from one point in the image at a time. Sure, you could pay lots
of $$ for a big CCD with the resolution you desire, or you could simply
digitize the one (or more) video signals as the beam scans the screen. You
have the choice of either asserting the x-y positions through active
digital control or you can read them off passively.

From a hardware perspective, it is a much easier (and cheaper) task to
build an active or passive system like those on the market than to build a
4x5 camera back detector. For both systems you would still need the
software and computer

At 10:58 PM 5/17/2000 -0700, Gary Gaugler wrote:

} I can identify with your situation. While I do not know the intricacies
} of the JEOL instruments, some SEMs are made to accept external
} drive for x-ray analysis. This same input scheme works perfectly
} for active control of the SEM and direct digital capture of images.
} At the worst, you can replicate the resolution of your record CRT
} using a passive mode. How easy either of these modes are depends
} greatly on how the SEM system was designed.
}
} gary g.
}
} At 01:44 PM 5/17/00, you wrote:
} }
} } I'm no digital imaging guru, but I have been following this thread with
} } interest. This whole discussion has been of interest to me because of the
} } idea of the passive 4x5 (camera back) "detector" that could provide an
} } extremely simple user venue for converting those countless analog SEMs into
} } a very usable digital output format.
} }
} } We have a 10 year old JEOL 840 microanalysis system that we would like to
} } see in operation for another 10 years, and I think it may be possible.
} } However, digital imaging, although "doable", is not "convenient" for us, at
} } this point for this instrument. Too much time and effort is required in
} } digital acquisition, maintenance of instrument condition info with the
} } image, labeling the image, and archiving. Then on top of it all, the
} } digital image is often not quite as good as the analog Polaroid shot that
} } also has the Mag, Bar Scale, KeV, WD, and other info permanently
} } incorporated (We use Type 53 for much of our work.) Don't get me wrong, we
} } do some digital imaging with it, but "it ain't the same" as using a newer
} } digital scope with integrated digital control and digital interface. If
} } someone put such a passive digital detector into a 4x5 camera back-type
} } mount, and it was capable of passively detecting at least 2500 horizontal
} } lines and an approximately equivalent vertical resolution, I believe it
} } would be a huge success with the analog SEMs. The 840 and many other
} } instruments like it are great conventional SEMs, and such a simple interface
} } (if affordable) would greatly extend their value. Many of these scanners
} } have exceptional imaging capability, but often the easiest/best way to
} } record that impressive image is by Polaroid film.
} }
} } Is it doable?
} } Brad Huggins



From daemon Thu May 18 23:02:54 2000



From: Bart Cannon :      cannonmp-at-accessone.com
Date: Thu, 18 May 2000 07:07:38 -0700
Subject: EDS and Mineral ID

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Hi Martin,

I fuss with mineral identification via EDS spectra every day. I keep
the program "Mineral" running at all times. It is a Windows Filemaker
Pro database which includes the chemical formula, physical properties,
type locale and 7 to 10 x-ray diffraction lines for 4500 accredited
mineral species and many un-named ones. Searches can be constructed
from any single or combination of fields. The software is available
from Aleph Enterprises in Livermore, CA (510 443 7319) The price was
around $550 a few years ago. Less expensive DOS based, but similar
software is available from the Fersmann Institute.

More complete chemistry (but no spectra) is offered by Alexander
Holzel's MDAT program. It includes a database of chemical analyses of
many minerals and can perform a search based on weight per cents of
elements present as well the unknown's physical properties and x-ray
diffraction data. This software is $1000 and up depending upon
options. Dr. Holzel's e-mail address was Compuserve100333,2771 as of
last August. He is in Ober-Olm, Germany.

None of the software programs work directly from spectra and only MDAT
contains sample analyses so you will likely be estimating peak heights
from chemical formula. For more definitive results you will need to
start building your own spectrum library from your own reference
materials.

Even with a high elemental analysis correlation, one will almost always
need supplemental methods for a definitive ID. This is usually x-ray
diffraction or optical microscopy.

Some day affordable EBSP retrofitted into SEMS will allow the analyst to
distinguish, in situ in polished section between orthorhombic FeS2
(marcasite) and cubic FeS2 (pyrite). Currently available systems are
$90,000+, I think.

If you need help obtaining grains of some of the less common minerals, I
can help. I supply a catalog which includes hundreds of well identified
reference quality mineral grains in addition to synthetic probe
standards.


Bart Cannon
Cannon Microprobe
1041 NE 100th Street
Seattle, WA 98125
206 522 9233 (3947 fax)



From daemon Thu May 18 23:03:08 2000



From: Sinkler, Wharton :      wharton.sinkler-at-anlw.anl.gov
Date: Thu, 18 May 2000 08:58:50 -0600
Subject: JEOL 2010: Electron dose from small screen reading

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Dear List,

In evaluating some electron beam damage data, I am trying to determine the
electron dose to the sample using a small screen current reading from a JEOL
2010 TEM.

The screen current density is given in pA/cm^2 on the microscope terminal,
and can be converted to dose (electrons/cm^2*s) in the sample. I have
spoken to JEOL, and they indicated that the actual current density on the
screen is the reading multiplied by a factor of 10.

I am unable to use a borrowed stage with a Farraday cup for calibration on
this microscope because it is suspect for internal radioactive
contamination. Thus, I would be grateful for insights from other users of
2010's who may have checked the accuracy of the reading (whether or not the
factor of 10 is right, etc.) on their instruments.

Many Thanks,
Wharton
--
++++++++++++++++++++++++++++++++++++++++++++++++++
Wharton Sinkler
Argonne National Laboratory West
P. O. Box 2528
Idaho Falls, ID 83403
Tel: (208) 533-7724



From daemon Thu May 18 23:03:10 2000



From: Kenneth JT Livi :      klivi-at-jhu.edu
Date: Thu, 18 May 2000 11:15:41 -0400
Subject: Re: EDS mineral identification and database

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Dear Martin,
As the other responders have stated, minerals can be difficult to identify
with EDS only. There is no substitute for experience (and some diffraction
data). My rule of thumb is to group minerals as do most mineral
classification schemes; is it a silicate, oxide, carbonate, sulfide,
phosphate, salt, organic, or metalic phase? Sometimes it's hard to decide
even at this level (i.e., If you don't have a light element detector, you
can't differentiate between oxides and carbonates, P and Si substitute for
each other). Most times you can. Since I often work with silicates, my
second observation is to look at Si/Al. This will help limit the choices
greatly, but is not usually diagnostic by itself. There are relatively few
minerals with Al} Si. Knowledge of the approximate Si/Al (the most common
tetrahedral cations) combined with the ratio of Si and Al with other
elements like Mg, Ti, Cr, Mn and Fe (sometimes Ca) will give you an idea of
the tetrahedral to octahedral ratio. The alkali elements Na and K, and also
Ca in significant abundances are very important for identifying feldspars
and sheet silicates, but again, there are no hard and fast rules that I've
come up with. The best thing is to know your mineral compositions (and
their structures) or hire a mineralogist!;-) I also agree that even the
mineralogist can find the DTSA program very useful and worth investing in a
MAC. Hope this helps.
Ciao for now,
Ken

Kenneth JT Livi
Department of Earth and Planetary Sciences
34th and Charles Streets
Johns Hopkins University
Baltimore, Maryland 21218 USA
Phone: (410) 516-8342
Fax: (410) 516-7933
e-mail: klivi-at-jhu.edu




From daemon Thu May 18 23:03:15 2000



From: Anpatoponiopo-at-aol.com
Date: Thu, 18 May 2000 11:51:32 EDT
Subject: LM - Need help choosing a new microscope.

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Due to the price of today's microscopes I am in need of advice concerning the
quality of Russian microscopes. I had one in Spain and it was excellent.
Please, advice.
Thanks, Jose



From daemon Thu May 18 23:03:19 2000



From: Winnie Westbrook :      ewwestbr-at-hsc.vcu.edu
Date: Thu, 18 May 2000 12:37:58 -0400
Subject: EM Safety Chart

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Hello Folks,
In reading the third edition of Hayat's Principles and Techniques of
Electron Microscopy, there was mention of a 'Safety Chart, Chemicals in
Electron Microscopy', for free distribution, by EMscope Laboratories Ltd
(Kingsnorth Industrial Estate, Ashford, Kent). Does anyone out there
have it? Or can anyone tell me how to get it or any wall chart that
lists chemicals/hazards specific to EM?
Thanks,
Winnie



From daemon Thu May 18 23:04:06 2000



From: Kenneth JT Livi :      klivi-at-jhu.edu
Date: Thu, 18 May 2000 13:47:52 -0400
Subject: Re: EDS mineral identification and database

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Lou,
Very true. However, not one instrument can unambigously identify ALL
minerals! In the case of my interests, PLM is not very useful since most of
the crystals in shale are less than 2 um. Martin's original question was
regarding how to identify EDS patterns, not what is the best way to
identify a mineral.
Ciao for now,
Ken

} Colleagues;
}
} My question is why have you forgotten that polarized light microscopes were
} devised for a need to identify and classify minerals? It almost seems
} intentional to ignore it. Perhaps I am beginning to sound like Dr. McCrone
} as I get older, but you dont have to throw a million dollar instrument at a
} problem to solve it. If people have a problem with a $20k light microscope
} doing a job better, that is there problem not mine. Just dont neglect the
} fact that PLM is still a powerful technique in the hands of a competent
} microscopist. PLM is still the standard for characterizing new crystal
} compounds at the be.....visit any pharmaceutical companys R&D department and
} you will see that it is so.
}
} Lou Solebello
}
}
} ----- Original Message -----
} From: Kenneth JT Livi {klivi-at-jhu.edu}
} To: {microscopy-at-sparc5.microscopy.com}
} Cc: {m.roe-at-mluri.sari.ac.uk}
} Sent: Thursday, May 18, 2000 8:15 AM
} Subject: Re: EDS mineral identification and database
}
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Dear Martin,
} } As the other responders have stated, minerals can be difficult to identify
} } with EDS only. There is no substitute for experience (and some diffraction
} } data). My rule of thumb is to group minerals as do most mineral
} } classification schemes; is it a silicate, oxide, carbonate, sulfide,
} } phosphate, salt, organic, or metalic phase? Sometimes it's hard to decide
} } even at this level (i.e., If you don't have a light element detector, you
} } can't differentiate between oxides and carbonates, P and Si substitute for
} } each other). Most times you can. Since I often work with silicates, my
} } second observation is to look at Si/Al. This will help limit the choices
} } greatly, but is not usually diagnostic by itself. There are relatively few
} } minerals with Al} Si. Knowledge of the approximate Si/Al (the most common
} } tetrahedral cations) combined with the ratio of Si and Al with other
} } elements like Mg, Ti, Cr, Mn and Fe (sometimes Ca) will give you an idea
} of
} } the tetrahedral to octahedral ratio. The alkali elements Na and K, and
} also
} } Ca in significant abundances are very important for identifying feldspars
} } and sheet silicates, but again, there are no hard and fast rules that I've
} } come up with. The best thing is to know your mineral compositions (and
} } their structures) or hire a mineralogist!;-) I also agree that even the
} } mineralogist can find the DTSA program very useful and worth investing in
} a
} } MAC. Hope this helps.
} } Ciao for now,
} } Ken
} }
} } Kenneth JT Livi
} } Department of Earth and Planetary Sciences
} } 34th and Charles Streets
} } Johns Hopkins University
} } Baltimore, Maryland 21218 USA
} } Phone: (410) 516-8342
} } Fax: (410) 516-7933
} } e-mail: klivi-at-jhu.edu
} }
} }
} }





From daemon Thu May 18 23:04:10 2000



From: Ladd Research :      ladres-at-worldnet.att.net
Date: Thu, 18 May 2000 14:48:44 -0400
Subject: Ladd Web Site

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Yes, we are aware of the problems in reaching our web site in the last few days. We have
posted our new web site of microscopy supplies at a new URL and it is now up and
running. Please change your bookmarks to:

http://www.laddresearch.com

Thank you,

John Arnott
--

LADD RESEARCH
131 Dorset Lane
Williston, VT 05495 USA

TEL 1-800-451-3406 (US) or 1-802-878-6711 (anywhere)
FAX 1-802-878-8074
e-mail ladres-at-worldnet.att.net

ALL NEW WEB SITE UP NOW AT OUR NEW URL:

http://www.laddresearch.com



From daemon Thu May 18 23:04:17 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Thu, 18 May 2000 14:33:54 -0700
Subject: Digitally enabling SEMs

Contents Retrieved from Microscopy Listserver Archives
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Warren's point is right on. The JEOL 840 or any other SEM
that has a record CRT for 4x5 film or Polaroid is the signal source
of passive information. It is not a big technical deal to do this.
Depending on how readily available the CRT signals are (blank, frame,
etc.), it may not be convenient or really simple. Some systems
use BNC connectors to snake the signals throughout the system.
T-ing off of these makes digitizing the SEM quite easy. If the
signals are hardwired, it is more work to install the capture
system. But in either case, it is a one time effort.

A passive system will record all legends just like the Polaroid
does. But it will do it a lot better. The reason is that the
tonal range and exposure latitude of a Polaroid is rather poor
compared to real film. Digital capture systems are typically
10-bits; or 12-bits for ones with really exceptional dynamic range.
Either of these are vastly superior to Polaroid prints.

The number of horizontal lines that a passive system will
capture is the same as a Polaroid. This is because the
record CRT circuitry fixes this dimension. However, the
scan rate alters the pixel dwell time. Slower scan rates
produce images that have less noise--be it Polaroid or
digital capture. If the Polaroid print works OK for you,
I would suggest that a digital capture system would be
even better.

Compared to the cost of a modern computerized SEM,
a digitizing attachment can be the key factor in keeping
a good old SEM.

gg

At 06:42 AM 5/18/00, you wrote:

} I will reiterate my point and that of others. An SEM technically has video
} only coming from one point in the image at a time. Sure, you could pay
} lots of $$ for a big CCD with the resolution you desire, or you could
} simply digitize the one (or more) video signals as the beam scans the
} screen. You have the choice of either asserting the x-y positions through
} active digital control or you can read them off passively.
}
} From a hardware perspective, it is a much easier (and cheaper) task to
} build an active or passive system like those on the market than to build
} a 4x5 camera back detector. For both systems you would still need the
} software and computer
}
} At 10:58 PM 5/17/2000 -0700, Gary Gaugler wrote:
}
} } I can identify with your situation. While I do not know the intricacies
} } of the JEOL instruments, some SEMs are made to accept external
} } drive for x-ray analysis. This same input scheme works perfectly
} } for active control of the SEM and direct digital capture of images.
} } At the worst, you can replicate the resolution of your record CRT
} } using a passive mode. How easy either of these modes are depends
} } greatly on how the SEM system was designed.
} }
} } gary g.
} }
} } At 01:44 PM 5/17/00, you wrote:
} } }
} } } I'm no digital imaging guru, but I have been following this thread with
} } } interest. This whole discussion has been of interest to me because of the
} } } idea of the passive 4x5 (camera back) "detector" that could provide an
} } } extremely simple user venue for converting those countless analog SEMs into
} } } a very usable digital output format.
} } }
} } } We have a 10 year old JEOL 840 microanalysis system that we would like to
} } } see in operation for another 10 years, and I think it may be possible.
} } } However, digital imaging, although "doable", is not "convenient" for us, at
} } } this point for this instrument. Too much time and effort is required in
} } } digital acquisition, maintenance of instrument condition info with the
} } } image, labeling the image, and archiving. Then on top of it all, the
} } } digital image is often not quite as good as the analog Polaroid shot that
} } } also has the Mag, Bar Scale, KeV, WD, and other info permanently
} } } incorporated (We use Type 53 for much of our work.) Don't get me wrong, we
} } } do some digital imaging with it, but "it ain't the same" as using a newer
} } } digital scope with integrated digital control and digital interface. If
} } } someone put such a passive digital detector into a 4x5 camera back-type
} } } mount, and it was capable of passively detecting at least 2500 horizontal
} } } lines and an approximately equivalent vertical resolution, I believe it
} } } would be a huge success with the analog SEMs. The 840 and many other
} } } instruments like it are great conventional SEMs, and such a simple interface
} } } (if affordable) would greatly extend their value. Many of these scanners
} } } have exceptional imaging capability, but often the easiest/best way to
} } } record that impressive image is by Polaroid film.
} } }
} } } Is it doable?
} } } Brad Huggins
}



From daemon Thu May 18 23:04:26 2000



From: prenitzer,brenda s :      prenitzer-at-lucent.com
Date: Thu, 18 May 2000 19:06:25 -0400
Subject: Room Temperature TEM Prep

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello all,

I am in need of a way to modify my current method of preparing TEM
cross-sections as to introduce no heating. Specifically, I need to find new
adhesive materials. We currently use thermally cured M-bond, Gatan G1 epoxy
and crystal bond wax - all of which require heat.

If anyone has information on an adhesive that ion mills at a rate to similar
Si, is stable under the electron beam, will cure at room temperature within
about 24 hours, and once cured is impervious to solvents such as acetone,
please respond. It would be an additional plus if the adhesive has low
viscosity, so it can be used to secure TEM grids to the specimens.


Also I am looking for a replacement for the wax that we currently use to
affix the samples to the polishing studs. This should cure quickly, bond
strongly enough to endure the mechanical stresses of grinding, and be
readily soluble in a solvent other than water so the samples can be removed
from the studs.

Any help would be greatly appreciated.

Brenda

Brenda I. Prenitzer, Ph.D.
Member of Technical Staff
Cirent Semiconductor (Lucent Technologies)
9333 S. John Young Parkway
6D-Lab
Orlando, FL 32819-8612

Phone: 407 371 7108
Fax: 407 371 6999

prenitzer-at-lucent.com
bsp101-at-worldnet.att.net



From daemon Thu May 18 23:04:28 2000



From: Hans Brinkies :      HBrinkies-at-groupwise.swin.edu.au
Date: Fri, 19 May 2000 10:49:39 +1000
Subject: Our management has finally agreed to connect my electron

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Our management has finally agreed to connect my electron microscopes to
a recirculating water system.

I need some ideas what type of systems are on the market and the
Australian agent for these units. We need to attach 2 SEMs, 1 TEM plus 1
coating unit to the new reciculating line.

Thank you

Hans Brinkies
Senior Lecturer
Swinburne, University of Technology
School of Engineering and Science
P.O.Box 218 - Hawthorn - Vic -3122 - Australia
Phone: +61 3 9214 8657
Fax: +61 3 9214 8264
Email: Hbrinkies-at-swin.edu.au



From daemon Thu May 18 23:04:28 2000



From: Mike Bode :      MikeB-at-Soft-Imaging.com
Date: Thu, 18 May 2000 19:08:31 -0600
Subject: RE: new developments in imaging systems?

Contents Retrieved from Microscopy Listserver Archives
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Sergey,

let me give you two more pieces of information:

1) Sensitivity: The CCD cameras are sensitive enough to see single
electrons striking the phosphor. You can't get much more sensitive than
that.

2) Price: I had a discussion about this with George McAuliffe in this
forum about that theme a while ago. That thread was also printed in
Microscopy Today. You may want to check the archives of this list server
for "digital archiving/cost". I think George would agree with me, that
the calculations of cost can swing in one or the other direction,
depending on how you define cost, what you need to include and how many
images you take (remember, a negative is on the order of $1 for the
material alone, not labor time or anything else).

Michael

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Sergey Ryazantsev [mailto:sryazant-at-ucla.edu]
Sent: Wednesday, May 17, 2000 1:06 PM
To: Microscopy-at-sparc5.microscopy.com


Thank you Mickhael.

I will wait for info about sensitivity. I think, for EM in particular,
sensitivity is very important parameter of the system. I am surprised
that
manufacturers don't have data on this matter. If sensitivity, say 10
times
higher than SO-163 film - it may be a great reason to switch from film
to
the CCD. The major disadvantage of the CCD cameras for TEM is their
price
in my point of view.
Thanks for your respond.

Sergey


} Date: Wed, 17 May 2000 11:34:05 -0600
} From: Michael Bode {mb-at-Soft-Imaging.com}
} Subject: RE: new developments in imaging systems?
} To: 'Sergey Ryazantsev' {sryazant-at-ucla.edu}
} X-Mailer: Internet Mail Service (5.0.1457.3)
}
} Sergey,
}
} I will try to find out what we have regarding the relative
} sensitivities. One thing that I can say now is, that we acquire images
} with a 50 or 100 msec exposure of the digital camera when the film
} requires an exposure of about 2 seconds. This would mean a 20 to 40
} times better sensitivity of the CCD camera. But to answer your question
} in more detail would require to compare also the resolution of film and
} camera and that is where it becomes very difficult, as it is not easy
to
} determine the resolution of film in terms of spatial resolution and
} dynamic range, as both are interwoven. I will try to find some answers
} for you.
}
} Regarding the linearity: As the CCDs simply count Photons, they have
} almost perfect linearity over their complete dynamic range. Even more
so
} for TEMs, where all Photons have the same energy and the quantum
} efficiency does not change from photon to photon. The Phosphor is a
part
} of a system that could theoretically introduce some non-linearities.
} However, I did measure the linearity of some TEM camera systems a few
} years back and did not find any significant deviations from linearity.
}
} Michael
}
} Michael Bode, Ph.D.
} Soft Imaging System Corp.
} 1675 Carr St., #105N
} Lakewood, CO 80215
} ===================================
} phone: (888) FIND SIS
} (303) 234-9270
} fax: (303) 234-9271
} email: mailto:info-at-soft-imaging.com
} web: http://www.soft-imaging.com
} ===================================
}
}
}
} -----Original Message-----
} From: Sergey Ryazantsev [mailto:sryazant-at-ucla.edu]
} Sent: Wednesday, May 17, 2000 1:29 AM
} To: Michael Bode
} Subject: RE: new developments in imaging systems?
}
}
} Mickhael hello
}
} I have question for you. I am thinking about adding CCD camera to my
} JEM1200EX. The information I gathered from Internet is not so
} optimistic.
} The standard CCD resolution is about 1-1.5 million pixels, 2 million is
} a
} max as I understand. The price for cheaper camera is about 20-30 K$ -
} much
} more that I expect to spend for "film" process. There are two things
} may
} attract me to the modern CCD camera: dynamic range and sensitivity. I
} am
} pretty sure that dynamic range for CCD itself is a few orders better
} than
} any film available. But what about phosphorus screen? Does it reduce
} dynamic range for the EM images? How dramatic? This is my first
} question.
} The next question is: could you tell me something about sensitivity of
} the
} modern CCD cameras used in EM? I am using dark field imaging at x80K
} magnification and the exposure time for SO-163 (non deluded D-19, 7
min,
} 20oC) is about 2 sec. I called GATAN, but they did not say anything
} useful.
} Could you provide some comparison of your side-mount camera with
} sensitivity of the SO-163 film at condition I mentioned? I will
greatly
} appreciate any information in this matter. Thanks. Sergey.
}
}
} } Date: Fri, 12 May 2000 13:46:01 -0600
} } From: Michael Bode {mb-at-Soft-Imaging.com}
} } Subject: RE: new developments in imaging systems?
} } To: "'Microscopy-at-MSA.Microscopy.Com'"
} {Microscopy-at-sparc5.microscopy.com}
} } Cc: 'Margaret Dienelt' {brannign-at-asrr.arsusda.gov}
} } X-Mailer: Internet Mail Service (5.0.1457.3)
} }
} } ----------------------------------------------------------------------
-
} -
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
}

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu
http://www.bol.ucla.edu/~sryazant



From daemon Fri May 19 22:06:37 2000



From: Ranan Gulhan Aktas :      ranaoz-at-usa.net
Date: Thu, 18 May 2000 23:14:06 -0500
Subject: Re: [Re-embedding paraffin sections in plastic]

Contents Retrieved from Microscopy Listserver Archives
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Dear Michelle;

A few months ago, I asked the same question and received the answers I copied
below. By the way, I would like to thank again to all who respond my
question. I do appreciate it. I still use these procedures and am planning
to prepare a study comparing these techniques. All of them work very well.

Hope these helps. Good luck.

Ranan Gulhan AKTAS, M.D.
Trakya University, Faculty of Medicine
Pathology Department
Edirne 22030
TURKEY

Tel: + 90 284 235 76 42
Fax: +90 284 235 76 52
e-mail: ranaoz-at-usa.net


Dear Dr. Ranan Gulhan Aktas
Protocol as follows:
De-wax in 100% xylene - small pieces of tissue - 1mm3 (3X)
Re-hydrate to water
xylene/ethanol 100% each
100% ethanol
96% ethanol
70% ethanol
H2O
GA in buffer} Normal EM processing from here on
Buffer }

Contact me if you have any further queries

Kind regards
John

Mr John F. Putterill
Electron Microscopy Unit Tel: (Int) 27-12-529-9174
Pathology Section Fax: (Int) 27-12-529-9165
Onderstepoort Veterinary Institute E-mail: john-at-moon.ovi.ac.za
Private Bag X05 http://www.ovi.ac.za
Onderstepoort 0110
South Africa

The basic procedure for re-embedding paraffin embedded is relatively
simple. We had a Lynx Tissue Processor set up to do it automatically.
Cut the paraffin tissue into 1-2 mm cubes with a warm scalpel. Trim
as much excess paraffin away as possible. De-paraffinize with 6
alternately warm/room temp xylene washes with agitation for 30 minutes
each. Reverse the dehydration process from 100% EtOH to your normal
EM buffer. Refix and postfix with appropriate EM fixatives,
dehydrate, and embed. The smaller the paraffin pieces, typically the
better the deparaffinization. Your tissue will never look great
though. The initial fixation for LM is a poor TEM fixative. good
Luck.

Chuck Butterick
Engineered Carbons, Inc.

Ranan,

Kai Chein did some nice work in this in the 1970's. He dissolved osmium
in xylene(1 percent)and then deparaffinized in that solution. This took
care of osmicating and depariffinization in one step (assuming you want
to use osmium). After several changes in this solution you can go right
into resin.. I've done this many times and it works very well and saves
a lot of time.


Tim Morken, B.S., EMT(MSA), HTL(ASCP)
Infectious Disease Pathology
Centers for Disease Control
MS-G32
1600 Clifton Rd.
Atlanta, GA 30333
USA

email: tim9-at-cdc.gov
timcdc-at-hotmail.com

FAX: (404)639-3043

MICROSCOPY AND IMAGING SERVICE CENTER
CELL BIOLOGY AND NEUROSCIENCES

PROCEDURE FOR PRELIMINARY PREPARATION OF PARAFFIN EMBEDDED TISSUE FOR ELECTRON
MICROSCOPY:


1. PLACE 0.5-1.0 mm CUBES OF TISSUE FROM PARAFFIN BLOCK IN XYLENE FOR
3 CHANGES OF 30 MINUTES EACH.

2. PUT IN 100% ETHANOL FOR 2 CHANGES OF 15 MINUTES WITH AGITATION.

3. PUT IN 95% ETHANOL FOR 2 CHANGES FOR 15 MINUTES WITH AGITATION.

4. PUT IN 70% ETHANOL FOR 2 CHANGES FOR 15 MINUTES WITH AGITATION.

5. PUT IN 50% ETHANOL FOR 2 CHANGES FOR 15 MINUTES WITH AGITATION.

6. RINSE IN PBS FOR 2 CHANGES OF 5 MINUTES EACH WITH AGITATION.

7. FOLLOW ROUTINE PREPARATION OF TISSUE FOR ELECTRON MICROSCOPY.

REFERENCE:

PIERCE, A., 1972. "A MANUAL FOR HISTOLOGICAL TECHNICIANS",
LITTLE AND BROWN, BOSTON.

Your morphology will not be great since the tissue was probably fixed in
formalin and
not glut. I've done this many times and have even had publications with this
method.
Make sure you get all of the paraffin out.


George Lawton
Chief Electron Microscopist
Microscopy and Imaging Service Center
UT Southwestern Medical Center at Dallas
Phone: 214-648-7291
eMail: George.Lawton-at-email.swmed.edu

Hi: This is Bob Mixon ( I work with Bob Kayton on the PNEMS society and am on
the
OHSU campus) . I have re-embedded tissues from paraffin many times and
many ways. I would recommend cutting out the piece of tissue from the
paraffin block and putting through at least two changes of xylene for
about 30 minutes each. Then you could put the tissue through either
absolute alcohol or acetone to remove the rest of the paraffin.
Probably 30 minutes and two changes. Some folks continue to run
the tissue through a series of alcohol and try to refix in EM fix
and post-fix in osmium. THIS IS FUTILE! I have never found any
enhancement by doing this. You can simply dissolve osmium in the
acetone and try fixing in this (two percent). and then running
through by your routine into plastic (through alcohol, acetone,
or propylene oxide etc).
Thanks Bob Mixon

Hello Ranan.

Here is the method used by the pathology people around here.

- Remove tissue of interest from parafin embedded specimen, cut in 1-2 mm
cubes.
- Leave the specimens in fresh 1% OsO4 in XYLENE for 1,5 - 2 hrs.
- Wash in xylene 2 times 10 min
- One part resin(we use Epon-Araldite) + two parts xylene for 1 hr.
- 1 part resin + 1 part xylene for 1 hr.
- Resin for 30 min to 1 hour without a lid on the glass

All this steps on a carousel.

Embedd as usual, but keep the specimens in the resin over night before
polymerization.

Good luck!

Best regards
Randi Olsen

Randi Olsen
Department of Electron Microscopy
Faculty of Medicine
University of Tromso
MH-Breivika
N-9037 TROMSO
NORWAY

tel: +47 77 64 53 15
fax: +47 77 64 46 50
email: randio-at-fagmed.uit.no

Hi Ranan,

The following procedure may sound a little obvious, but I imagine
you may be facing a rather limited access to all those books and
journals, and I would feel really happy if this could help.
I do wish you all the best in your EM lab-raising mission!

First, you will have to select smaller, "EM-size", portions of your
paraffin-embedded specimens, cut them out and thoroughly deparaffinate.
I would recommend at least 2 hours in xylene with frequent changes of
xylene and some agitation; you should be able to see when it's all gone.
Then several changes of 100% (anhydrous) ethanol, at least 1-1.5 h
total time, and then gradually down the ethanol concentrations, like
95-85-70-50, 30 min or more at each step.
Rinse 3x5 min in your choice of phosphate or cacodylate buffer, and
then osmicate for 1-2 h, 1% OsO4 in the same buffer. Then dehydrate
and embed for EM as you normally would.

Needless to say, even with the best original fixation the material
will look pityful, but most of those diagnostically significant
cell-to-cell junctions, filaments, etc. must still be there.

Best of luck!
Sincerely,
Vlad.

Vladislav V. Speransky
Postdoctoral Research Associate
School of Marine Sciences
University of Maine
5722 Deering Hall
Orono ME 04469-5722
Ph: 207 581 2998
FAX: 207 581 2969
Email: vladis-at-maine.maine.edu


Hi Keith,

At least for the specimens I happened to deal with so far (vertebrate
tissues, cell culture, bacteria, microalgae), the material normally will
not really blacken until you wash and start dehydrating it AFTER
osmication in traditional aqueous OsO4 solutions. While in aqueous OsO4,
it will remain brownish. (Unless, of course, you are using that
simultaneous, single solution glutaraldehyde/OsO4 fixation, or a
reducing buffer like PIPES, and have passed the time when it all
turns black...)

The explanation used to be that, at the stage of osmication, while some
of the OsO4, indeed, oxidizes nonsaturated lipids, etc., a lot of it
simply dissolves in the lipids of the specimen as nonreduced OsO4. Then,
say, the ethanol reduces that specifically accumulated OsO4, to form
the so called "osmium black".

As for making FRESH solutions of OsO4 in xylene (or CCl4, etc.),
I would just never have a spare OsO4 ampule (and enough paraffine
blocks to EM-reevaluate) to try that! :-)

Sincerely,
Vlad.

Vladislav V. Speransky
Postdoctoral Research Associate
School of Marine Sciences
University of Maine
5722 Deering Hall
Orono, ME 04469-5722
Ph: 207 581 2998
FAX: 207 581 2969
Email: vladis-at-maine.maine.edu

} I was interested in the use of osmium in xylene that you described. Does
the tissue blacken as
} in a water-solution or does it simply become yellowish/brown? I am curious
because the latter is
} what happens in the absence of water during freeze-substitution using e.g.
1% in acetone at -80
} *C, although the colour change probably happens when warming up from low
temperature.


Hello Keith.

According to the people here that most often works with this (Irene Lund)
the blocks are not as dark as with standard methods, but light brown. We
haven't done freeze-substitution with osmium, so it's not easy to compare
directly.
For this purpose we buy ampoulas with 0,1 gram OsO4.

Best regards
Randi Olsen
Randi Olsen
Department of Electron Microscopy
Faculty of Medicine
University of Tromso
MH-Breivika
N-9037 TROMSO
NORWAY

tel: +47 77 64 53 15
fax: +47 77 64 46 50
email: randio-at-fagmed.uit.no

Hi Keith,

At least for the specimens I happened to deal with so far (vertebrate
tissues, cell culture, bacteria, microalgae), the material normally will
not really blacken until you wash and start dehydrating it AFTER
osmication in traditional aqueous OsO4 solutions. While in aqueous OsO4,
it will remain brownish. (Unless, of course, you are using that
simultaneous, single solution glutaraldehyde/OsO4 fixation, or a
reducing buffer like PIPES, and have passed the time when it all
turns black...)

The explanation used to be that, at the stage of osmication, while some
of the OsO4, indeed, oxidizes nonsaturated lipids, etc., a lot of it
simply dissolves in the lipids of the specimen as nonreduced OsO4. Then,
say, the ethanol reduces that specifically accumulated OsO4, to form
the so called "osmium black".

As for making FRESH solutions of OsO4 in xylene (or CCl4, etc.),
I would just never have a spare OsO4 ampule (and enough paraffine
blocks to EM-reevaluate) to try that! :-)

Sincerely,
Vlad.

Vladislav V. Speransky
Postdoctoral Research Associate
School of Marine Sciences
University of Maine
5722 Deering Hall
Orono, ME 04469-5722
Ph: 207 581 2998
FAX: 207 581 2969
Email: vladis-at-maine.maine.edu

Dear HistoNetters,

Dr. Aktas asked about the re-embedding of paraffin embedded
tissues in resin for observation by electron microscopy. There
are two papers at our web site which describe this "Pop-Off" technique.
Go to the URL provided in my signature file and follow the links to the
JB-4 microtomy system, then to "Technical Information"

If anyone needs reprints of these articles and has no WWW
access, send a note to Sonja White in my office (sales-at-ebsciences.com)
{mailto:sales-at-ebsciences.com)} , and she'll s-mail you the dead tree
version.

Best regards,
Steven E. Slap, Vice-President

*******************************************
Energy Beam Sciences, Inc.
The Laboratory Microwave Company
Adding Brilliance to Your Vision
http://www.ebsciences.com {http://www.ebsciences.com}
Ranan,

Kai Chein did some nice work in this in the 1970's. He dissolved osmium
in xylene(1 percent)and then deparaffinized in that solution. This took
care of osmicating and depariffinization in one step (assuming you want
to use osmium). After several changes in this solution you can go right
into resin.. I've done this many times and it works very well and saves
a lot of time.


Tim Morken, B.S., EMT(MSA), HTL(ASCP)
Infectious Disease Pathology
Centers for Disease Control
MS-G32
1600 Clifton Rd.
Atlanta, GA 30333
USA

email: tim9-at-cdc.gov
timcdc-at-hotmail.com

FAX: (404)639-3043
The basic procedure for re-embedding paraffin embedded is relatively
simple. We had a Lynx Tissue Processor set up to do it automatically.
Cut the paraffin tissue into 1-2 mm cubes with a warm scalpel. Trim
as much excess paraffin away as possible. De-paraffinize with 6
alternately warm/room temp xylene washes with agitation for 30 minutes
each. Reverse the dehydration process from 100% EtOH to your normal
EM buffer. Refix and postfix with appropriate EM fixatives,
dehydrate, and embed. The smaller the paraffin pieces, typically the
better the deparaffinization. Your tissue will never look great
though. The initial fixation for LM is a poor TEM fixative. good
Luck.

Chuck Butterick
Engineered Carbons,

Inc."Michelle.Taurino-at-aventis.com"-at-sparc5.microscopy.com wrote:
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Any suggestions on re-embedding paraffin sections in epon?
Has anyone worked with the "pop out" method using BEEM capsules?

Any suggestions would be helpful...

Michelle Taurino
Aventis Pharmaceuticals
Bioimaging and Molecular Histology
Michelle.Taurino-at-aventis.com
908-231-3357
"Michelle.Taurino-at-aventis.com"-at-sparc5.microscopy.com wrote:
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Any suggestions on re-embedding paraffin sections in epon?
Has anyone worked with the "pop out" method using BEEM capsules?

Any suggestions would be helpful...

Michelle Taurino
Aventis Pharmaceuticals
Bioimaging and Molecular Histology
Michelle.Taurino-at-aventis.com
908-231-3357


____________________________________________________________________
Get free email and a permanent address at http://www.netaddress.com/?N=1




From daemon Fri May 19 22:06:44 2000



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Thu, 18 May 2000 22:03:36 -0700
Subject: RE: new developments in imaging systems?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Jim hello

I was talking about very special EM case: dark-field TEM. At this point we
are talking about a few electrons per square angstrom, even less. In high
resolution EM crystallography people have deal with 0.6 e/A2. At such
conditions, high sensitivity and linearity of the modern digital cameras
may be a plus. Talking about long exposures --what about drift? I never
had good pictures at x100K with exposure longer that a few seconds. I
don't understand your point about noise. In case of digital camera the
noise is a function of camera. Current cameras has a very low level of
noise (they uses cooling, etc) and we have to pay for that astronomical
price. This is a life. I am not friendly with TEM cameras, but I do know
that in the light microscopy people count individual photons using CCD
cameras. In TEM camera we have phosphorous screen as a source of image and
my questions to Mickhael were addressed mostly to the problem how
effectively (and correctly) information is traveled thought that funny
screen. The Mickhael's answer is that screen does not affect dynamic range
and sensitivity is much higher than on convention films. Am I correct,
Mickhaels?

Sergey

} Date: Thu, 18 May 2000 14:15:02 +1000
} From: jim {jim-at-proscitech.com.au}
} Subject: RE: new developments in imaging systems?
} To: 'Sergey Ryazantsev' {sryazant-at-ucla.edu} ,
} "Microscopy-at-sparc5.microscopy.com" {Microscopy-at-sparc5.microscopy.com}
} Reply-to: "jim-at-proscitech.com.au" {jim-at-proscitech.com.au}
} Organization: ProSciTech
} X-Mailer: Microsoft Internet E-mail/MAPI - 8.0.0.4211
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu
http://www.bol.ucla.edu/~sryazant




From daemon Fri May 19 22:06:49 2000



From: Dr Malcolm Roberts :      malc-at-rock.ru.ac.za
Date: Fri, 19 May 2000 11:35:37 +0200
Subject: BSE resolution

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi
We have a JEOL 733 with a two-sector PN type BS detector. Anyone out
there have any idea on the resolution of these things in terms of mean
atomic number. What I want to know basically is the sensitivity of these
things to compositional variation.
Cheers,
Malc.

--
Dr MP Roberts Phone: [+27](0)46 603 8313
Dept of Geology Fax: [+27](0)46 622 9715
Rhodes University Cell: 083 4060 262 (usually off)
6140 Grahamstown e-mail: malc-at-rock.ru.ac.za
SOUTH AFRICA




From daemon Fri May 19 22:06:56 2000



From: Martin J. Roe :      m.roe-at-mluri.sari.ac.uk
Date: Fri, 19 May 2000 10:12:33 +0000
Subject: EDS mineralogy/database

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thanks for all the responses to this posting both on and off the
list.
I routinely analyse minerals found in soils and reservoir
sandstones and can easily recognise most of the common
minerals by their EDS spectra. The problem for me recently
has been looking at samples containing some of the more
'exotic' minerals, or put simply minerals I'm not used to
looking at, e.g. sphene and clinpyroxenes. This has been more
of a problem because we had no supporting XRD data for these
particular analyses.
What I was asking for, perhaps naively, was a program that
would help identify an unknown mineral from its spectrum and
give a list of possible alternatives. This is evidently not
available.
What is available:
Thereis a free Mac program DTSA that can simulate
spectra of a mineral for a particular detector and kV etc.
Scott Wight/Mark Blackford). This can be downloaded
from http://www.cstl.nist.gov/div837/837.02/dtsa.html
Thereis a EDS spectrum database with search
capabilities (based on archived spectra) written for the
US FBI which may be commercially available soon. I
will certainly follow up this up soon (thanks Dennis
Ward and Nicholas Ritchie.)
Thereseems to be various databases including MDAT
that allow a search by chemical elements but not actual
analyses. (thanks Bart Cannon)
About a year ago, I started building a spectrum library
collected on our system recording the various beam
operating parameters, preparation (rough or polished, C or
Au coated) etc. I agree with Bart Cannon that this is
probably the best way forward. Just one thing - my database
needs many more spectra added to it, especially different
variations of many of the solid solution minerals. I suppose
the idea of the database is that it should be used as a
reference guide only. Although some minerals could never
be identified by their EDS spectrum alone this is where an
extensive reference library of my own would come in so
useful and help answer the question: is this spectrum a Ca
feldspar or Ca-rich zeolite? Although the best answer to this
specific question would probably be the unknown spectrum
indicates it may be a Zeolite because it is more similar to
the zeolite reference we collected under similar operating
parameters. Of course there would be no substitute if there
were XRD data telling you in the same sample of zeolite and
no Ca-feldspar.
Thanks again to all of you who responded.
Best regards
Martin Roe

Martin J. Roe
Macaulay Land Use Research Institute
Craigiebuckler
Aberdeen
Scotland
UK
Phone 01224 318611
e-mail m.roe-at-mluri.sari.ac.uk



From daemon Fri May 19 22:07:01 2000



From: Frank Thomas :      thomasf-at-AGC.BIO.NS.CA
Date: Fri, 19 May 2000 08:10:49 -0300
Subject: Re: Our management has finally agreed to connect my electronmicroscopes to a recirculating water system

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




----------
} From: Hans Brinkies {HBrinkies-at-groupwise.swin.edu.au}
} To: microscopy-at-sparc5.microscopy.com
} Subject: Our management has finally agreed to connect my
electronmicroscopes to a recirculating water system
} Date: May 18, 2000 9:49 PM
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Our management has finally agreed to connect my electron microscopes to
} a recirculating water system.
}
} I need some ideas what type of systems are on the market and the
} Australian agent for these units. We need to attach 2 SEMs, 1 TEM plus 1
} coating unit to the new reciculating line.
}
} Thank you
}
} Hans Brinkies
} Senior Lecturer
} Swinburne, University of Technology
} School of Engineering and Science
} P.O.Box 218 - Hawthorn - Vic -3122 - Australia
} Phone: +61 3 9214 8657
} Fax: +61 3 9214 8264
} Email: Hbrinkies-at-swin.edu.au
}
} I don't really know what companies distribute such systems in Australia,
but one thing occurs to me....if you set up all your instruments on one
circulator, it's nice and cost-effective, but when the pump crashes or
motor burns out, all your toys are down until the one circulation system is
repaired. Separate systems for each 'scope would be better, (that'll
probably give your managers a heart attack) or at least try and retain the
capability of switching back to the old constant-loss system during
emergencies. Our own ESEM was recently down for nearly three weeks while I
was trying to replace the circulator pump motor. Turned out to be kind of a
hard one to find...

Frank Thomas
MicroAnalysis Facility
Geological Survey of Canada Atlantic
Bedford Institute of Oceanography
Dartmouth, Nova Scotia



From daemon Fri May 19 22:07:05 2000



From: Ron Doole :      ron.doole-at-materials.oxford.ac.uk
Date: Fri, 19 May 2000 13:37:01 +0100 (GMT Daylight Time)
Subject: Philips heating holders for TEM

Contents Retrieved from Microscopy Listserver Archives
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Hi All,

For the past 20 years I have used the Philips TEM heating
holder furnace and heater elements to build hotstages for a
range of microscopes. Unfortunately, Philips have told me
that they can no longer supply the Pt furnace bodies and I
cannot get information on possible replacements.

If anyone has a spare furnace (or more?) - Philips part
number 5322 265 70028 - that they wish to part with I am
willing to pay a `Philips' price. I am also willing to
consider lightly used furnaces or complete holders that are
now surplus to requirements and will pay a price depending
on condition and history.

If anyone has any information on replacements to the
previous style or on present day heaters I would be most
grateful.

Thanks,
Ron

----------------------
Mr. R.C. Doole
Department of Materials,
University of Oxford.
Parks Road, Oxford. OX1 3PH. UK.
Phone +44 (0) 1865 273701
Fax +44 (0) 1865 283333
ron.doole-at-materials.ox.ac.uk



From daemon Fri May 19 22:07:03 2000



From: White, Woody N :      Woody.N.White-at-mcdermott.com
Date: Fri, 19 May 2000 08:03:00 -0500
Subject: Re:BSE resolution

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Atomic number contrast sensitivity using BSE is dependent on several
variables
in addition to the equipment. I may not cover everything, but what quickly
comes to mind....

Specimen surface condition - If a specimen is well polished, compo imaging
is
best. Topography of rough specimens (like a fracture) will generate
feature
contrast, "diluting" the compo contrast.

Beam current and potential affect sensitivity.

Conductive films will reduce sensitivity. Use carbon rather than sputtered
Au,
Pd etc. to minimize the effect if the specimen must be coated.

Depending on the detector geometry, working distance can affect the solid
angle
of collection thus changing BSE collection effiency.

The absloute atomic numbers present in the specimen will affect sensitivity
in
two ways.

Sensitivity will be different for a composition of lighter elements compared
to
a specimen composed of higher atomic numbers.

Also, if it is desired to NOT saturate the video, the range of atomic
numbers
can limit sensitivity. This is a typically a result of limited dynamic
range in
the image capture device/photo. For example, if C and W are present as pure
inclusions in a brass, it will be difficult (to say the least) to contrast
the
difference between Cu and Zn phases whild not loosing C & W contrast
variability.

Considering the above, any single statement of sensitivity is difficult, but
if
I had to generalize... My 4 diode GW Electronics BSE system will resolve
0.1
atomic number differencees under "good" conditions.

Woody White
McDermott Technology


------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Hi
We have a JEOL 733 with a two-sector PN type BS detector. Anyone out
there have any idea on the resolution of these things in terms of mean
atomic number. What I want to know basically is the sensitivity of these
things to compositional variation.
Cheers,
Malc.

--
Dr MP Roberts Phone: [+27](0)46 603 8313
Dept of Geology Fax: [+27](0)46 622 9715
Rhodes University Cell: 083 4060 262 (usually off)
6140 Grahamstown e-mail: malc-at-rock.ru.ac.za
SOUTH AFRICA



From daemon Fri May 19 22:07:35 2000



From: Walck, Scott D. :      walck-at-ppg.com
Date: Fri, 19 May 2000 12:28:19 -0400
Subject: RE: Room Temperature TEM Prep

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Sergey and others,

I want to add my 2 cents as I like the topic.
Most of this is a result of my experience with our TEM 2Kx2K CCD.
We are working in bright field - so I cannot comment on dark field
performance
Several points:

1. The CCD is much more convenient - you get your pictures instantly.

2. CCD is linear and has larger dynamic range than the film.

3. The bad thing about the CCD is resolution - about 4 times lower than that
of the film (in our camera the pixel size is 30 microns). So if you want to
work in minimum dose you will do better with film. As I know the CCD is not
performing well in terms of signal to noise at low doses (and if you have to
work at 4 times higher magnification because of the resolution the things
become much worse).

4. The CCD has smaller observation area - again loss of information.

5. I don't know about the detection efficiency compared to the film - it
depends on the thickness of the phosphorous and the accelerating voltage. If
a photon reaches the CCD chip it will be detected ... the problems are in
the conversion electron-photon. There are two sides - if you make the
phosphorous thicker you will get higher detection efficiency but the point
spread also increases so always a compromise is made between detection
efficiency and resolution. When I say detection efficiency this is not only
related to the detection of single electrons (as it detects single
electrons) but more to the actual signal detected on the background of the
noise. Apart from the shot noise additional noise is added due to the
scintillator driven detection and thermal noise in the CCD chip.

The CCDs are now very popular in diffraction work because of the dynamic
range and linearity.

Here is one reference where a nice comparison between 2Kx2K CCD and film has
been made:

Downing, K., Hendrickson, F., Ultramicroscopy, 75, (1999), 215-233

Best regards,

Rado

---------------------------------------------------------------------
Radostin Danev
Laboratory of Ultrastructure Research
National Institute for Physiological Sciences
Myodaiji-cho, Okazaki 444-8585, JAPAN
e-mail: rado-at-nips.ac.jp
---------------------------------------------------------------------
----- Original Message -----
} From: Sergey Ryazantsev {sryazant-at-ucla.edu}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Friday, May 19, 2000 2:03 PM


If your samples are not site specific, then I would recommend a modified
version of the small angle cleavage technique. You can get a detailed
description of this technique in the MRS TEM Sample Prep IV book, vol 480.

Instead of the low temperature melting wax to hold the samples down, you use
superglue. It takes longer to soak the samples off in acetone, but there is
no heat. Then instead of the silver epoxy that is normally used, you need
to use a slow curing viscous epoxy to mount the samples on the copper grid.
There may be a temperature spike in the curing, but you would have to
experiment and find out yourself. The amount of epoxy that is needed is
very small and I doubt that it would raise the temperature an appreciable
amount. The down side is that it will take a day to fully cure the epoxy.
The net result is that the only heating the sample really sees is the heat
generated during grinding the back side of the samples down.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)



} -----Original Message-----
} From: prenitzer,brenda s [mailto:prenitzer-at-lucent.com]
} Sent: Thursday, May 18, 2000 7:06 PM
} To: 'List Server'
} Subject: Room Temperature TEM Prep
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListse} rver/FAQ.html
}
}
} --------------------------------------------------------------
} ---------.
}
}
} Hello all,
}
} I am in need of a way to modify my current method of preparing TEM
} cross-sections as to introduce no heating. Specifically, I
} need to find new
} adhesive materials. We currently use thermally cured M-bond,
} Gatan G1 epoxy
} and crystal bond wax - all of which require heat.
}
} If anyone has information on an adhesive that ion mills at a
} rate to similar
} Si, is stable under the electron beam, will cure at room
} temperature within
} about 24 hours, and once cured is impervious to solvents such
} as acetone,
} please respond. It would be an additional plus if the
} adhesive has low
} viscosity, so it can be used to secure TEM grids to the specimens.
}
}
} Also I am looking for a replacement for the wax that we
} currently use to
} affix the samples to the polishing studs. This should cure
} quickly, bond
} strongly enough to endure the mechanical stresses of grinding, and be
} readily soluble in a solvent other than water so the samples
} can be removed
} from the studs.
}
} Any help would be greatly appreciated.
}
} Brenda
}
} Brenda I. Prenitzer, Ph.D.
} Member of Technical Staff
} Cirent Semiconductor (Lucent Technologies)
} 9333 S. John Young Parkway
} 6D-Lab
} Orlando, FL 32819-8612
}
} Phone: 407 371 7108
} Fax: 407 371 6999
}
} prenitzer-at-lucent.com
} bsp101-at-worldnet.att.net
}
}



From daemon Fri May 19 22:07:48 2000



From: Barbara Plowman :      Bplowman-at-sfmail.dental.uop.edu
Date: Fri, 19 May 2000 11:38:20 -0700
Subject: Amoeba

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am about to do a TEM prep on amoeba. Does anyone out there have any information on a fixation procedure? Thank you!



From daemon Fri May 19 22:07:49 2000



From: Barbara Plowman :      Bplowman-at-sfmail.dental.uop.edu
Date: Fri, 19 May 2000 11:44:18 -0700
Subject: Amoeba

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am about to do a TEM prep on amoeba. Does anyone out there have any information on a fixation procedure? Thank you

Barbara Plowman
University of the Pacific
School of Dentistry
2155 Webster
San Francisco
email: Bplowman-at-sfuop.edu



From daemon Fri May 19 22:07:52 2000



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Fri, 19 May 2000 13:27:11 -0600
Subject: RE: new developments in imaging systems?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Yes,

as I said in my response, a direct comparison between the sensitivities
of film and a CCD camera are very complicated. The film has a non-linear
response curve, it reacts to electrons directly, there is grain size to
take into account, etc. All I wanted to say is the following: For a film
camera setting of 2 seconds, we acquire an image in approx. 50 to 100
msec with similar contrasts to that of film.

Regarding the linearity of the phosphor-CCD system: I have measured the
linearity by measuring the beam current and the image intensity and I
found it to be linear over the entire range that I measured. The
deviations were insignificant and probably due to noise (I would give
you the numbers, but this was in a different life and I don't have
them). There may be nonlinearities that show up if you reach extreme
illumination levels of the phosphor, but I did not measure them and it
will probably depend on the phosphor itself.

Michael

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Sergey Ryazantsev [mailto:sryazant-at-ucla.edu]
Sent: Thursday, May 18, 2000 11:04 PM
To: Microscopy-at-sparc5.microscopy.com


Jim hello

I was talking about very special EM case: dark-field TEM. At this point
we
are talking about a few electrons per square angstrom, even less. In
high
resolution EM crystallography people have deal with 0.6 e/A2. At such
conditions, high sensitivity and linearity of the modern digital cameras
may be a plus. Talking about long exposures --what about drift? I
never
had good pictures at x100K with exposure longer that a few seconds. I
don't understand your point about noise. In case of digital camera the
noise is a function of camera. Current cameras has a very low level of
noise (they uses cooling, etc) and we have to pay for that astronomical
price. This is a life. I am not friendly with TEM cameras, but I do
know
that in the light microscopy people count individual photons using CCD
cameras. In TEM camera we have phosphorous screen as a source of image
and
my questions to Mickhael were addressed mostly to the problem how
effectively (and correctly) information is traveled thought that funny
screen. The Mickhael's answer is that screen does not affect dynamic
range
and sensitivity is much higher than on convention films. Am I correct,
Mickhaels?

Sergey

} Date: Thu, 18 May 2000 14:15:02 +1000
} From: jim {jim-at-proscitech.com.au}
} Subject: RE: new developments in imaging systems?
} To: 'Sergey Ryazantsev' {sryazant-at-ucla.edu} ,
} "Microscopy-at-sparc5.microscopy.com" {Microscopy-at-sparc5.microscopy.com}
} Reply-to: "jim-at-proscitech.com.au" {jim-at-proscitech.com.au}
} Organization: ProSciTech
} X-Mailer: Microsoft Internet E-mail/MAPI - 8.0.0.4211
}
} -----------------------------------------------------------------------
-
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From daemon Fri May 19 22:07:58 2000



From: Margaret Mitchell :      MMitchell-at-aoac.org
Date: Fri, 19 May 2000 16:13:45 -0400
Subject: Need Help on microscopy, etc. techniques used to authenticate art

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,

I am new to this list. I would like to know if anyone has information I
could use to write an article on the analytical chemistry techniques used to
authenticate art objects using light microscopes or any other type of
microscope. Also, if anyone has information on any of the other techniques
used to authenticate art objects such as x-ray diffraction, x-ray
fluorescence, accelerator mass spectrometry, that would be helpful too.

Sincerely,

Margaret M. Mitchell
Assistant Editor
AOAC INTERNATIONAL
301-924-7077 (tel)
301-924-7089 (fax)
mmitchell-at-aoac.org



From daemon Fri May 19 22:08:12 2000



From: john david whitaker :      jwhitake-at-u.washington.edu
Date: Fri, 19 May 2000 14:01:02 -0700 (PDT)
Subject: final polishing for metals (Cu, Ni, Fe, NiFe)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I'm looking for information regarding final polishing
solutions for preparing metallic cross-section samples
for TEM analysis.

I've looked into colloidal silica
suspensions such as Ludox and Syton, and am concerned
that their alkilinity may chemically etch my samples.
I'm analyzing different compositions of NiFe
on copper substrates, so the specimens are prone to
preferential etching of different phases.

Does anyone have any experience with this?

Any input would be appreciated.

John



From daemon Fri May 19 22:08:21 2000



From: Sally Stowe :      STOWE-at-rsbs.anu.edu.au
Date: Sat, 20 May 2000 11:31:36 +1000
Subject: Position Open

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Please reply to email addresses listed in the advertisement. More
information available on:
http://www.anu.edu.au/hr/jobs/ext.html

THE AUSTRALIAN NATIONAL UNIVERSITY
RESEARCH SCHOOL OF BIOLOGICAL SCIENCES
PLANT CELL BIOLOGY GROUP

ANU Officer Grade 7 OR 8 (Research)
Grade 7:$43,506 - $47,010 (Ref. G001411)
Grade 8: $48,696 - $54,146 per annum (Ref. G001410)


Reference: G001410 OR G001411. A position is available for a Head of
Advanced Light Microscopy Facilities in the Biological Sciences at ANU. The
appointee will be responsible for the maintenance of advanced light
microscope equipment in the Research School of Biological Sciences and John
Curtin School of Medical Research and for the training of users of this
equipment. The appointee will be expected to play a major role in the
identification of novel techniques in light microscope imaging and in the
preparation of applications for the procurement of new light microscope
equipment. Experience in biological imaging, computational image processing
and maintenance of opto-electronic systems is required.

The appointment will be made in the Plant Cell Biology Group in the
Research School of Biological Sciences as a continuing position. The
position will become available in July 2000.


Contacts: Professor Adrienne R. Hardham: Telephone 61 2 6249 4168,
FAX 61 2 6249 4331, Email Hardham-at-RSBS. ANU.edu.au.
Professor Bruce Walmsley: Telephone 61 2 6249 2039, FAX 61 2 6249 2687,
Email Bruce.Walmsley-at-ANU.edu.au.


Selection criteria are available from Susan Toscan. Telephone 61 2 6249
4752; FAX 61 2 6249 4891; Email Susan.Toscan-at-ANU.edu.au


Closing date: 12 June 2000

Applications addressing the selection criteria should be submitted to Susan
Toscan, RSBS, ANU, Canberra 2601 ACT quoting reference number and including
curriculum vitae, list of publications and names and addresses of at least
three referees.




From daemon Sun May 21 00:35:31 2000



From: jim :      jim-at-proscitech.com.au
Date: Sat, 20 May 2000 12:29:07 +1000
Subject: RE: Our management has finally agreed to connect my electron microscopes to a recirculating water system

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


With that agreement you also have a few new problems to consider. I suggest
that:
In Australia you could not buy a large system like that as a supplied item.
Several smaller plants would be much too expensive and cumbersome.
Any imported system will result in problems with fittings and parts

I expect that it would be best (been there, done that a couple of times) to
design your own large system with a bit of spare capacity.

Beer chillers as used in hotels are most suitable. You want at least two large
chillers, so if one fails you still could run more than half of the equipment).
Calculate total heater element wattage with some spare capacity.

Diffusion pumps are designed to work best at 16 degrees. At that temperature
the TEM column will condense water. Depending on ambient temperature, it might
be sufficient to use the dif pump return line to cool the column and so avoid
that problem. The chillers should be installed under an open shelter as they
generate a lot of heat. Chiller temperature cut-in and out requires a
thermostat each.

I suggest that a centrifugal pump of 1hp capacity will give enough pressure at
the relative small flow rates. Chose a very common pump make so you can install
a spare quickly without replacing fittings. Other pump types give more
pressure, but they are more expensive and generally less reliable.

12mm reinforced garden hoses with hose clamps to and from the instruments make
installation easy, pretty secure and cause minimal pressure drop.

Required is a SS tank (insulated) or a fiberglass lined tank to act as a heat
sink. 100 liters would be a reasonable minimum capacity.

A good inline filter is required and a bypass, so the system can run during
filter change.

If a mains water line is maintained, it is very important to have the returning
water going to a drain whenever mains water is in use. I have lived through
some horrid floods, which resulted from people opening mains but not the bypass
to the drain.

If you have the components any plumber can install the system in under a day.
The plumber would supply gate valves, temperature and pressure gauges etc.

Hans, you may fax your rough design to me, I'll be happy to make any
suggestions.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Friday, May 19, 2000 10:50 AM, Hans Brinkies
[SMTP:HBrinkies-at-groupwise.swin.edu.au] wrote:
}
} Our management has finally agreed to connect my electron microscopes to
} a recirculating water system.
}
} I need some ideas what type of systems are on the market and the
} Australian agent for these units. We need to attach 2 SEMs, 1 TEM plus 1
} coating unit to the new reciculating line.
}
} Thank you
}
} Hans Brinkies
} Senior Lecturer
} Swinburne, University of Technology
} School of Engineering and Science
} P.O.Box 218 - Hawthorn - Vic -3122 - Australia
} Phone: +61 3 9214 8657
} Fax: +61 3 9214 8264
} Email: Hbrinkies-at-swin.edu.au
}



From daemon Fri May 19 22:08:24 2000



From: Edward Hirsch :      edhirsch-at-att.net
Date: Fri, 19 May 2000 22:15:31 -0500
Subject: final polishing for metals (Cu, Ni, Fe, NiFe)

Contents Retrieved from Microscopy Listserver Archives
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John,
The solutions that the colloidal silica are in have a ph of 8.5 to 9.8
depending on the formulation. I suggest you try our 0.05 micron water based
polycrystalline diamond suspension. Allied High Tech
http://www.alliedhightech.com sells this product. I would also suggest our
Final A cloth for this polishing step. If you would like samples of either
of these products please let me know and I will be happy to send it to you.

If you need further technical assistance please contact me off-line and I
will be happy to help or you may contact our main office at (800)675-1118
located in CA.

I hope this helps.
Ed
Please note, I have a financial interest in providing you with these
products and other sample preparation equipment and consumable items.

*************************************************
Edward A. Hirsch
Product Application Specialist
Allied High Tech Products
2376 East Pacifica Place
Rancho Dominguez, CA 90220
ph: (919) 846-9628
vm:(800)675-1118 x245
fx: (310)762-6808
http://www.alliedhightech.com

Equipment and Consumables for Metallurgical Sample Preparation
*************************************************

-----Original Message-----
} From: john david whitaker [mailto:jwhitake-at-u.washington.edu]
Sent: Friday, May 19, 2000 4:01 PM
To: Microscopy Lister Server


I'm looking for information regarding final polishing
solutions for preparing metallic cross-section samples
for TEM analysis.

I've looked into colloidal silica
suspensions such as Ludox and Syton, and am concerned
that their alkilinity may chemically etch my samples.
I'm analyzing different compositions of NiFe
on copper substrates, so the specimens are prone to
preferential etching of different phases.

Does anyone have any experience with this?

Any input would be appreciated.

John





From daemon Sun May 21 02:25:56 2000



From: Rosemary Walsh :      rw9-at-psu.edu
Date: Sun, 21 May 2000 01:06:53 -0500
Subject: Pt shadowing of ultrasmall gold

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers,
Would Pt shadowing of a protein-DNA complex interfere with an
ultrasmall
gold label on the protein?
Rosemary




From daemon Sun May 21 02:25:56 2000



From: ComCryLab1-at-aol.com
Date: Sun, 21 May 2000 01:11:08 -0500
Subject: EM: Help

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Friends,
Can anyone give me advice regarding the dismantling and
the packing for shipment of a Philips 201 TEM? I need to
pay particular attention to not disturbing the alignment.
I plan to move the TEM from Univ. of Delaware to Naples, Florida
in an enclosed U-Haul Trailer. Any help or suggestions
will be most welcome.
If anyone has hands-on experience in doing this sort of thing
and is willing to give me a hand at U-Del, please contact me
and name your price. I'll gladly pay for the assistance.
Best regards,
Mike Urbanik
www.crystalguru.com




From daemon Mon May 22 00:40:17 2000



From: jim :      jim-at-proscitech.com.au
Date: Sun, 21 May 2000 18:38:27 +1000
Subject: RE: new developments in imaging systems?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I find what you are trying to do interesting, but there are things that may
never work and this may be one.

We had it confirmed by Michael Bode that good digital system "see "single
photons - so that is not likely to improve much. Film too registers single
electrons. We were told that in digital 1 electron can expose one pixel. On
film one electron initiates the exposure of a silver halide and subsequent
electrons in a near identical location increase the size of the grain.

Low noise (cooled cameras) hopefully do not add their own noise, but they
cannot make up for lack of information in the image.

My case (below) was that in high resolution TEM at least, less exposed images
will be inferior because there are simply not enough electrons to produce a
good image. A more sensitive digital system uses fewer electrons and so makes
matters worse.

In dark field EM we are using the beam indirectly and such images too suffer
particularly from electron noise. Here too the use of a digital system would
only make this worse. Furthermore, the slower 4489 film would be better than
SO-163.

If you don't like long exposures (4 to 8 seconds I found a practical limit),
you cannot ignore the reality of electron noise and hope to correct that with
yet fewer electrons, albeit processed in a "noise-free" digital system.
I think that the answer is more electrons, i.e. a field emission source or a
slower digital camera.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Friday, May 19, 2000 3:04 PM, Sergey Ryazantsev [SMTP:sryazant-at-ucla.edu]
wrote:
}
}
} Jim hello
}
} I was talking about very special EM case: dark-field TEM. At this point we
} are talking about a few electrons per square angstrom, even less. In high
} resolution EM crystallography people have deal with 0.6 e/A2. At such
} conditions, high sensitivity and linearity of the modern digital cameras
} may be a plus. Talking about long exposures --what about drift? I never
} had good pictures at x100K with exposure longer that a few seconds. I
} don't understand your point about noise. In case of digital camera the
} noise is a function of camera. Current cameras has a very low level of
} noise (they uses cooling, etc) and we have to pay for that astronomical
} price. This is a life. I am not friendly with TEM cameras, but I do know
} that in the light microscopy people count individual photons using CCD
} cameras. In TEM camera we have phosphorous screen as a source of image and
} my questions to Mickhael were addressed mostly to the problem how
} effectively (and correctly) information is traveled thought that funny
} screen. The Mickhael's answer is that screen does not affect dynamic range
} and sensitivity is much higher than on convention films. Am I correct,
} Mickhaels?
}
} Sergey
}
} } Date: Thu, 18 May 2000 14:15:02 +1000
} } From: jim {jim-at-proscitech.com.au}
} } Subject: RE: new developments in imaging systems?
} } To: 'Sergey Ryazantsev' {sryazant-at-ucla.edu} ,
} } "Microscopy-at-sparc5.microscopy.com" {Microscopy-at-sparc5.microscopy.com}
} } Reply-to: "jim-at-proscitech.com.au" {jim-at-proscitech.com.au}
} } Organization: ProSciTech
} } X-Mailer: Microsoft Internet E-mail/MAPI - 8.0.0.4211
} }
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } If sensitivity was ten times higher, one would in some cases save some beam
} } damage and in most cases suffer excessive electron noise.
} } As a rough guide, electron noise becomes apparent at 30x enlargement,
} rarely a
} } problem. Magnification is a linear function and electron density relates
} to an
} } area, but clearly very short exposure images would be much noisier and
} could
} } not be enlarged nearly as much. When not enough electrons form an image it
} } appears grainy, which makes it unsuitable for further enlarging. Photo
} } enlarging utilises higher depths-of-field and without this, very high
} power TEM
} } is much, much harder.
} } By nature, slower emulsions have finer grain and higher resolution and
} } contrast. It is fortuitous that these desirable characteristics run in
} tandem
} } with relatively long exposures, so the image is formed by more electrons.
} } TEM films rate about 6 ISO, so X-ray film at about ISI 3000 is about a 10x
} } faster than TEM films. Unfortunately its rather grainy, but if the
} exposure was
} } well adjusted, chances are that electron noise would be more bothersome than
} }
} } the film's grain.
} } Digital is inevitable and already fairly common, but some aces remain with
} } conventional film.
} } Cheers
} } Jim Darley
} } ProSciTech Microscopy PLUS
} } PO Box 111, Thuringowa QLD 4817 Australia
} } Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
} } Great microscopy catalogue, 500 Links, MSDS, User Notes
} } www.proscitech.com
} }
} } On Thursday, May 18, 2000 5:06 AM, Sergey Ryazantsev
} [SMTP:sryazant-at-ucla.edu]
} } wrote:
} } }
} } }
} } } Thank you Mickhael.
} } }
} } } I will wait for info about sensitivity. I think, for EM in particular,
} } } sensitivity is very important parameter of the system. I am surprised
} } } that
} } } manufacturers don't have data on this matter. If sensitivity, say 10
} } } times
} } } higher than SO-163 film - it may be a great reason to switch from film to
} } } the CCD. The major disadvantage of the CCD cameras for TEM is their price
} } } in my point of view.
} } } Thanks for your respond.
} } }
} } } Sergey
} } }
} } }
} } } } Date: Wed, 17 May 2000 11:34:05 -0600
} } } } From: Michael Bode {mb-at-Soft-Imaging.com}
} } } } Subject: RE: new developments in imaging systems?
} } } } To: 'Sergey Ryazantsev' {sryazant-at-ucla.edu}
} } } } X-Mailer: Internet Mail Service (5.0.1457.3)
} } } }
} } } } Sergey,
} } } }
} } } } I will try to find out what we have regarding the relative
} } } } sensitivities. One thing that I can say now is, that we acquire images
} } } } with a 50 or 100 msec exposure of the digital camera when the film
} } } } requires an exposure of about 2 seconds. This would mean a 20 to 40
} } } } times better sensitivity of the CCD camera. But to answer your question
} } } } in more detail would require to compare also the resolution of film and
} } } } camera and that is where it becomes very difficult, as it is not easy to
} } } } determine the resolution of film in terms of spatial resolution and
} } } } dynamic range, as both are interwoven. I will try to find some answers
} } } } for you.
} } } }
} } } } Regarding the linearity: As the CCDs simply count Photons, they have
} } } } almost perfect linearity over their complete dynamic range. Even more so
} } } } for TEMs, where all Photons have the same energy and the quantum
} } } } efficiency does not change from photon to photon. The Phosphor is a part
} } } } of a system that could theoretically introduce some non-linearities.
} } } } However, I did measure the linearity of some TEM camera systems a few
} } } } years back and did not find any significant deviations from linearity.
} } } }
} } } } Michael
} } } }
} } } } Michael Bode, Ph.D.
} } } } Soft Imaging System Corp.
} } } } 1675 Carr St., #105N
} } } } Lakewood, CO 80215
} } } } ===================================
} } } } phone: (888) FIND SIS
} } } } (303) 234-9270
} } } } fax: (303) 234-9271
} } } } email: mailto:info-at-soft-imaging.com
} } } } web: http://www.soft-imaging.com
} } } } ===================================
} } } }
} } } }
} } } }
} } } } -----Original Message-----
} } } } From: Sergey Ryazantsev [mailto:sryazant-at-ucla.edu]
} } } } Sent: Wednesday, May 17, 2000 1:29 AM
} } } } To: Michael Bode
} } } } Subject: RE: new developments in imaging systems?
} } } }
} } } }
} } } } Mickhael hello
} } } }
} } } } I have question for you. I am thinking about adding CCD camera to my
} } } } JEM1200EX. The information I gathered from Internet is not so
} } } } optimistic.
} } } } The standard CCD resolution is about 1-1.5 million pixels, 2 million is
} } } } a
} } } } max as I understand. The price for cheaper camera is about 20-30 K$ -
} } } } much
} } } } more that I expect to spend for "film" process. There are two things
} } } } may
} } } } attract me to the modern CCD camera: dynamic range and sensitivity. I
} } } } am
} } } } pretty sure that dynamic range for CCD itself is a few orders better
} } } } than
} } } } any film available. But what about phosphorus screen? Does it reduce
} } } } dynamic range for the EM images? How dramatic? This is my first
} } } } question.
} } } } The next question is: could you tell me something about sensitivity of
} } } } the
} } } } modern CCD cameras used in EM? I am using dark field imaging at x80K
} } } } magnification and the exposure time for SO-163 (non deluded D-19, 7 min,
} } } } 20oC) is about 2 sec. I called GATAN, but they did not say anything
} } } } useful.
} } } } Could you provide some comparison of your side-mount camera with
} } } } sensitivity of the SO-163 film at condition I mentioned? I will greatly
} } } } appreciate any information in this matter. Thanks. Sergey.
} } } }
} } } }
} } } } } Date: Fri, 12 May 2000 13:46:01 -0600
} } } } } From: Michael Bode {mb-at-Soft-Imaging.com}
} } } } } Subject: RE: new developments in imaging systems?
} } } } } To: "'Microscopy-at-MSA.Microscopy.Com'"
} } } } {Microscopy-at-sparc5.microscopy.com}
} } } } } Cc: 'Margaret Dienelt' {brannign-at-asrr.arsusda.gov}
} } } } } X-Mailer: Internet Mail Service (5.0.1457.3)
} } } } }
} } } } } -----------------------------------------------------------------------
} } } } -
} } } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } }
} } } } } To Subscribe/Unsubscribe -- Send Email to
} } } } ListServer-at-MSA.Microscopy.Com
} } } } } On-Line Help
} } } } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } } } -----------------------------------------------------------------------
} } } } .
} } } } }
} } } } }
} } } } } Margaret,
} } } } }
} } } } } As a former user and current vendor of such systems as you are
} } } } inquiring
} } } } } about I can try to provide a bit of information regarding camera
} } } } } improvements:
} } } } }
} } } } } There have been a number of improvements, but I am not sure what you
} } } } are
} } } } } comparing the latest cameras against. Cameras are now usually cooled
} } } } and
} } } } } provide 12 bits per pixel, the number of pixels has gone up a bit (but
} } } } } not much in general), and cameras read out faster than they used to (up
} } } } } to 20 fps and more). I think all cameras now use a line transfer
} } } } } mechanism, which makes shutters obsolete.
} } } } } On the software side, real-time FFT and real-time shading correction
} } } } can
} } } } } be done now due to faster computers without special processing boards,
} } } } } and there have been other software developments that make using the
} } } } } cameras and computers easier.
} } } } } Other changes that affect the usability of cameras is the use of
} } } } } pneumatics to insert and retract the phosphors, higher frame rates for
} } } } } live viewing with the camera, etc.
} } } } }
} } } } } If you have questions, please give me a call, drop me an email, or go
} } } } to
} } } } } our web site.
} } } } }
} } } } } Michael
} } } } }
} } } } }
} } } } } Michael Bode, Ph.D.
} } } } } Soft Imaging System Corp.
} } } } } 1675 Carr St., #105N
} } } } } Lakewood, CO 80215
} } } } } ===================================
} } } } } phone: (888) FIND SIS
} } } } } (303) 234-9270
} } } } } fax: (303) 234-9271
} } } } } email: mailto:info-at-soft-imaging.com
} } } } } web: http://www.soft-imaging.com
} } } } } ===================================
} } } } }
} } } } }
} } } } }
} } } } } -----Original Message-----
} } } } } } From: Margaret Dienelt [mailto:brannign-at-asrr.arsusda.gov]
} } } } } Sent: Thursday, May 11, 2000 12:43 PM
} } } } } To: Microscopy-at-sparc5.microscopy.com
} } } } } Subject: TEM: new developments in imaging systems?
} } } } }
} } } } }
} } } } } -----------------------------------------------------------------------
} } } } -
} } } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } }
} } } } } To Subscribe/Unsubscribe -- Send Email to
} } } } ListServer-at-MSA.Microscopy.Com
} } } } } On-Line Help
} } } } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } } } -----------------------------------------------------------------------
} } } } .
} } } } }
} } } } }
} } } } } Hi,
} } } } }
} } } } } Year after year I hopefully gather information about digital imaging
} } } } } systems for TEMs (ours is a JEOL 100CX) , only to learn we have no
} } } } } money. This year it looks like it might really happen but I have not
} } } } } kept up with innovations in the field and am wondering the following:
} } } } }
} } } } } 1. Anything new in the last two years -- especially in terms of
} } } } } cameras? I'm most familiar with the Gatan and AMT systems but their
} } } } } web sites don't reflect much in the way of changes over a year ago.
} } } } } 2. With more and more microscopists finally getting their systems --
} } } } } I'd love to get feedback.
} } } } }
} } } } } Thanks,
} } } } } Margaret
} } } } }
} } } } } P.S. Would welcome contacts from vendors.
} } } } }
} } } } } --
} } } } } Margaret Dienelt
} } } } }
} } } } } Plant Pathologist
} } } } } Electron Microscopy Lab
} } } } }
} } } } } Floral and Nursery Plants Research Unit
} } } } } U.S. National Arboretum/Agricultural Research Service/USDA
} } } } }
} } } } } B. 010A, Rm. 238, BARC-W
} } } } } 10300 Baltimore Avenue
} } } } } Beltsville MD. 20705 USA
} } } } }
} } } } } (301) 504-6097
} } } } } Fax: (301) 504-5096
} } } } }
} } } } }
} } } } _____________________________________
} } } }
} } } } Sergey Ryazantsev Ph. D.
} } } } Electron Microscopy
} } } } UCLA School of Medicine
} } } } Department of Biological Chemistry
} } } } Box 951737
} } } } Los Angeles, CA 90095-1737
} } } }
} } } } Phone: (310) 825-1144
} } } } Pager: (310) 845-0248
} } } } FAX (departmental): (310) 206-5272
} } } } mailto:sryazant-at-ucla.edu
} } } } http://www.bol.ucla.edu/~sryazant
} } } }
} } } }
} } } _____________________________________
} } }
} } } Sergey Ryazantsev Ph. D.
} } } Electron Microscopy
} } } UCLA School of Medicine
} } } Department of Biological Chemistry
} } } Box 951737
} } } Los Angeles, CA 90095-1737
} } }
} } } Phone: (310) 825-1144
} } } Pager: (310) 845-0248
} } } FAX (departmental): (310) 206-5272
} } } mailto:sryazant-at-ucla.edu
} } } http://www.bol.ucla.edu/~sryazant
} } }
} } }
} }
} }
} }
} _____________________________________
}
} Sergey Ryazantsev Ph. D.
} Electron Microscopy
} UCLA School of Medicine
} Department of Biological Chemistry
} Box 951737
} Los Angeles, CA 90095-1737
}
} Phone: (310) 825-1144
} Pager: (310) 845-0248
} FAX (departmental): (310) 206-5272
} mailto:sryazant-at-ucla.edu
} http://www.bol.ucla.edu/~sryazant
}
}



From daemon Mon May 22 00:40:19 2000



From: Victor Sidorenko :      antron-at-space.ru
Date: Mon, 22 May 2000 01:10:41 +0400
Subject: Re: BSE resolution

Contents Retrieved from Microscopy Listserver Archives
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Dear Malc!
Some years ago I got the resolution 0.1 in the middle of periodic
table (around Z=28) on standard 733 BSE.
But for this purpose it is necessary to balance additionally the
preamplifier to remove completely the rest of topo signal, which
becomes comparable by value with a very low signal of differential
compo. There are no means in the preamplifier for this additional
balancing, therefore I have soldered the additional variable resistor
in one of shoulders of a differential amplifier in the preamplifier.
Frankly speaking I have forgotten the details already, but I can
restore them, if you wait about two weeks. But basically it is very
simple.
Regards.
Victor Sidorenko, ANTRON Co. Ltd., scientific service, Moscow, Russia.

-----é–›”Š’”Œ –””‡Œ’Œ-----
ë˜: Dr Malcolm Roberts {malc-at-rock.ru.ac.za}
攓—: Microprobe discussion group {Microscopy-at-sparc5.microscopy.com}
€ˆ˜ˆ: 19 “ˆØ 2000 ‹. 16:13
񌓈: BSE resolution


} ---------------------------------------------------------------------
---
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
America



From daemon Mon May 22 00:40:19 2000



From: Baggethun, Paul :      Paul.Baggethun-at-alcoa.com
Date: Sun, 21 May 2000 17:42:46 -0400
Subject: RE: final polishing for metals (Cu, Ni, Fe, NiFe)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


John,

Polishing suspensions that does not interact chemically with your samples
include diamond suspensions (available down to 0.1µm at least) and alumina
suspensions (down to 0.05µm). Many vendors have information available on the
World-Wide-Web.

Cheers,
Paul
===================
Paul Baggethun
Alcoa Technical Center
Alcoa Center, PA 15069
USA
===================
+ 724 - 337-1760 (tel)
+ 724 - 337-2044 (fax)
===================


} ----------
} From: john david whitaker[SMTP:jwhitake-at-u.washington.edu]
} Sent: Friday, May 19, 2000 5:01 PM
} To: Microscopy Lister Server
} Subject: final polishing for metals (Cu, Ni, Fe, NiFe)
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I'm looking for information regarding final polishing
} solutions for preparing metallic cross-section samples
} for TEM analysis.
}
} I've looked into colloidal silica
} suspensions such as Ludox and Syton, and am concerned
} that their alkilinity may chemically etch my samples.
} I'm analyzing different compositions of NiFe
} on copper substrates, so the specimens are prone to
} preferential etching of different phases.
}
} Does anyone have any experience with this?
}
} Any input would be appreciated.
}
} John
}
}



From daemon Mon May 22 00:40:24 2000



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Sun, 21 May 2000 13:40:29 -1000 (HST)
Subject: Re: TEM: Help

Contents Retrieved from Microscopy Listserver Archives
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On Thu, 18 May 2000 ComCryLab1-at-aol.com-at-sparc5.microscopy.com wrote:

} Dear Friends,
} Can anyone give me advice regarding the dismantling and
} the packing for shipment of a Philips 201 TEM? I need to
} pay particular attention to not disturbing the alignment.
} I plan to move the TEM from Univ. of Delaware to Naples, Florida
} in an enclosed U-Haul Trailer. Any help or suggestions
} will be most welcome.

I will make sure Ron Veil, a very experienced independent EM service tech,
sees this and has a chance to reply. It was with his advice that we (my
hubby and I and its new owner) packed up and shipped a Philips 201. Like
you, we wanted to ship it intact, column on and all. We built a heavy
duty skid and added a strong base to which we bolted the instrument. Then
we wrapped it with whatever that plastic packing tape that is like Saran
Wrap is called, making sure to secure any part of the column we didn't
want to move, but avoiding putting any pressure on things, such as the
aperture drives. Wrap the bottom, wrap the column, wrap the column to the
bottom and back again, etc. Build a crate up around the instrument.
I think I remember putting a wood insert with a crescent cut out near the
top of, but not touching the column, but hubby thinks not. The idea is
NOT to let any shock to the crate get transferred to the column, so
perhaps we didn't. Add some packing material, such as old egg crate foam
from your bed (it needed replacing anyway) and whatever. Then, and this
was the fun part, buy that stuff that when you mix it with a catalyst,
produces huge volumes of foam that hardens in a few minutes. I think you
can also buy spray cans of similar stuff, but since hubby had this on hand
for other things, we had the bulk stuff. Fill the voids in the crate with
it. It will easily chip off later. Add a top, and away you go. The
scope made it in great shape.

I think we packed the rotary pump and a couple of other things
separately. Get it into the truck and TIE IT DOWN. I say this because an
SEM we once packed for shipping with the base and saran, but no closed
crate, made it from Hawaii to Ohio, then slid in the shippers truck in the
last mile and slammed into the driver's seat. The driver was OK, the ion
getter pump was bent, and the column needed a bit of work, but it could
have been worse. Duh.

I've also packed up a couple of Denton vcuum evaporators and a couple of
ultramicrotomes. The saran stuff and blow foam are a good way to
stabilize the instruments.

Good luck!

Aloha,
Tina

http://www.pbrc.hawaii.edu/bemf/microangela
****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************



From daemon Mon May 22 00:40:24 2000



From: Gordon Couger :      gcouger-at-rfdata.net
Date: Sun, 21 May 2000 22:51:58 -0500
Subject: Re: TEM: Help

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} From: "Tina Carvalho" {tina-at-pbrc.hawaii.edu}
} On Thu, 18 May 2000 ComCryLab1-at-aol.com-at-sparc5.microscopy.com wrote:
}
} } Dear Friends,
} } Can anyone give me advice regarding the dismantling and
} } the packing for shipment of a Philips 201 TEM? I need to
} } pay particular attention to not disturbing the alignment.
} } I plan to move the TEM from Univ. of Delaware to Naples, Florida
} } in an enclosed U-Haul Trailer. Any help or suggestions
} } will be most welcome.
}
} I will make sure Ron Veil, a very experienced independent EM service tech,
} sees this and has a chance to reply. It was with his advice that we (my
} hubby and I and its new owner) packed up and shipped a Philips 201. Like
} you, we wanted to ship it intact, column on and all. We built a heavy
} duty skid and added a strong base to which we bolted the instrument. Then
} we wrapped it with whatever that plastic packing tape that is like Saran
} Wrap is called, making sure to secure any part of the column we didn't
} want to move, but avoiding putting any pressure on things, such as the
} aperture drives. Wrap the bottom, wrap the column, wrap the column to the
} bottom and back again, etc. Build a crate up around the instrument.
} I think I remember putting a wood insert with a crescent cut out near the
} top of, but not touching the column, but hubby thinks not. The idea is
} NOT to let any shock to the crate get transferred to the column, so
} perhaps we didn't. Add some packing material, such as old egg crate foam
} from your bed (it needed replacing anyway) and whatever. Then, and this
} was the fun part, buy that stuff that when you mix it with a catalyst,
} produces huge volumes of foam that hardens in a few minutes. I think you
} can also buy spray cans of similar stuff, but since hubby had this on hand
} for other things, we had the bulk stuff. Fill the voids in the crate with
} it. It will easily chip off later. Add a top, and away you go. The
} scope made it in great shape.
}
} I think we packed the rotary pump and a couple of other things
} separately. Get it into the truck and TIE IT DOWN. I say this because an
} SEM we once packed for shipping with the base and saran, but no closed
} crate, made it from Hawaii to Ohio, then slid in the shippers truck in the
} last mile and slammed into the driver's seat. The driver was OK, the ion
} getter pump was bent, and the column needed a bit of work, but it could
} have been worse. Duh.
}
} I've also packed up a couple of Denton vcuum evaporators and a couple of
} ultramicrotomes. The saran stuff and blow foam are a good way to
} stabilize the instruments.
}
Since you are using a U haul you don't have to worry about sides
and a top on the crate. Make sure it is tied down real well. Dry
wall screws through the crate and into the floor work well for
locking it down but make sure you have it braced with timbers
to the front and sides of the trailer in case you make a panic
stop.

Also make sure that the weight is centers in front of the trailer
axle if you use a trailer. Negitive weight on the trailer tounge
at the very least makes driving very interesting. You have no
Idea how fast a trailer can pass you while it is still tied on to the
truck. I have had the privilege of experiencing this and I can promise
you that you won't enjoy it:).

The foam in place stuff is great. Just cover the instrument in plastic
and foam away. If you can get foam between the instrument and the
Support the foam will dissipate most of those little shocks that get
things out of line.

Last of all make sure you understand what you insurance covers and
what it doesn't on moving equipment. You should be fine on liability but
the value of the contents is probably not covered. Rental truck companies
have contents insurance as an extra.

Good luck
Gordon

Gordon Couger gcouger-at-couger.com
Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00







From daemon Mon May 22 10:19:51 2000



From: NIKONBIO-at-aol.com -at-oldsparc5.Microscopy.Com
Date: Mon, 22 May 2000 00:55:45 -0500
Subject: JOB ANNOUNCEMENT

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hello,

I would like to announce an exciting job opportunity to the Microscopy
community.


JOB ANNOUNCEMENT

POSITION TITLE: SR. SCIENTIST/ENGINEER LIGHT MICROSCOPY NIKON INC.
MELVILLE, NY
Posted: May 18, 2000

As seen in the 19 May issue of Science:
http://recruit.sciencemag.org/cgi/show/5469/5469x03207

Sr. Applications Scientist/Engineer
Bring Your Light Microscopy
Experience To The BioScience Division Of Nikon Inc.

Our world renowned company has an excellent opportunity for a professional
with extensive experience in light microscopy, specifically in advanced
BioScience technologies in our USA headquarters located in Melville, Long
Island , NY. Today, scientists at world-renowned biological institutions are
making tremendous advances in Cancer, AIDS, Alzheimer's, in-vitro
fertilization and other leading-edge research. Nikon is proud to be playing a
role in this enormously important work. Our ongoing commitment to optical
excellence and technological advancement is allowing researchers to view
objects never before seen by the human eye. We would like to invite you to
investigate the new opportunities and technologies available at Nikon Inc.
BioSciences.


JOB SUMMARY

Provide the principal conduit for technical information and feedback between
the end user/sales network and Nikon Factory engineers on: product
applications, product acceptance, competitive changes, required product
improvements, new technologies and methodologies that impact Nikon's present
and future business. Provide the sales force with technical assistance to
successfully satisfy customer's needs and complete the sale. Create and
conduct training curriculum on advanced microscopy and new technologies to
Nikon distribution and end users. Attend trade shows, workshops and national
dealer / sales meetings. Write technical and applications bulletins to end
users and distribution channels as well articles for publication in Bio
journals highlighting Nikon technology or applications.


Selected candidate will act as liaison for technical information and feedback
between the end user/sales network and Nikon factory engineers on all matters
pertaining to our BioScience technical product line. Applicants must have a
Ph.D. in BioSciences, BioEngineering or Physics with a specialty in optics,
or equivalent experience. Knowledge of advanced microscopy techniques and
optical principals including fluorescence applications, imaging and confocal
applications required. Excellent communications skills in English a must,
along with good writing and public speaking abilities. Excellent benefits and
compensation provided.


Mail or fax your resume, which MUST include salary requirements, to:
HR Department, Nikon, 1300 Walt Whitman Road, Melville, NY 11747. Fax:
631-547-4025.

Or send a digital cover letter and resume / CV in (plain text, MS Word, or
.pdf file) to biosales-at-nikonincmail.com

Check our website: www.nikonusa.com.

Also See add display in NY Times:
http://search.nytimes.com/classified/display/adverts/524986401/

Nikon is an Equal Opportunity Employer
=============================================================

Best Regards,

Stan Schwartz
Manager BioSciences Dept.
Nikon Inc.
1300 Walt Whitman Rd.
Melville, NY 11747
631-547-8500




From daemon Tue May 23 00:29:37 2000



From: lherault :      lherault-at-bu.edu
Date: Mon, 22 May 2000 02:56:09 -0400
Subject: Sputter coating artifact?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I've had to coat some PLGA polymer samples and we think the coater may be
melting them. The samples have a rolled or beaded edge and large cracks. I
have jpeg images if anyone wants me to send them for a look. Are we
correct in our assessment or should we look elsewhere for the source of
cracks, shrinkage, beading around edges. These artifacts, BTW do not appear
when the uncoated samples are checked under a light microscope.

Thanks in advance.

Ron L
lherault-at-bu.edu



From daemon Mon May 22 10:19:52 2000



From: Shu-You Li :      syli-at-mail.uni-mainz.de
Date: Mon, 22 May 2000 10:46:58 +0200
Subject: TEMalert - A pre-print anouncement system for TEM region.

Contents Retrieved from Microscopy Listserver Archives
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Dear friends,
I have just finished writing the programs for TEMAlert system(http://www6.ewebcity.com/temalert), which serves as a pre-print announcement system. Currently it serves only TEM-related region. Do you think this is very useful for all TEM users? The system was designed to be totally self-maintaining and everyone can post messages to announce his/her newly published (accepted) paper there.

I hope that TEMAlert will become a widespread blackboard in TEM region and tighten the relationship between electron microscopists allover the world.

I am sorry that the server is somewhat slow - because it is a free internet host. I will be very grateful if anyone of you can supply me a space (should support ASP).

With best regards,
Shu-You Li
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Please visit my new homepage - a personal website on transmission electron microscopy (TEM) - at http://syli.homepage.com at your convenience!
It contains
my current research work, my resume
TEM-related journals, instruction for authors
link to on-line EELS database, periodic table, physical constants
JOB list and RESUMEs (only for TEM region)
TEMAlert - a self-maintaining preprint announcement system
**************************************************
Shu-You Li, Dr.
Institut fuer Physikalische Chemie
Johannes Guttenberg Universitaet
Jakob-Welder-Weg 11
D-55099 Mainz, Germany

E-mail: syli-at-mail.uni-mainz.de; syli16-at-hotmail.com
Fax: +49-6131-3923768
Tel: +49-6131-3923148(O)
**************************************************






From daemon Mon May 22 10:19:53 2000



From: ard-at-ansto.gov.au (Arthur Day)
Date: Mon, 22 May 2000 20:49:43 +1000
Subject: RE: Our management has finally agreed to connect my electron microscopes to a

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hans, Jim,

} In Australia you could not buy a large system like that as a supplied item.

Don't know about that...We've had excellent results from a locally made
system we've been using here now on the SEMs for several years. Service and
parts are readily available and it has heaps of spare capacity. Contact
Mark Blackford (mgb-at-postoffice.ansto.gov.au) for details on who to contact
about this system because they probably have a branch in Melbourne.

}
} Beer chillers as used in hotels are most suitable.

Certainly a mass produced item in Australia.



Arthur Day, Electron Microscope Unit Phone: 61-2-9717-3457
Ansto Materials Division Fax: 61-2-9543-7179
PMB 1, Menai (Sydney), NSW, 2234 Email: ard-at-ansto.gov.au
Australia www: http://www.ansto.gov.au/




From daemon Mon May 22 10:19:56 2000



From: Dr. Edgar Voelkl :      vog-at-ornl.gov
Date: Mon, 22 May 2000 09:55:45 -0400
Subject: video from single images?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


hello,

I have a batch of images (~300) that I would like to present as a
QuickTime movie. Does anybody have experience how to do this? I am
using a Macintosh. Comments welcome. Thanks,

Edgar


________________
Dr. Edgar Voelkl
ORNL
Bldg 4515
1 Bethel Valley Road
P.O. Box 2008
Oak Ridge, TN 37831-6064

Tel.: (865) 574-8181
Fax: (865) 574-4913
email: vog-at-ornl.gov



From daemon Mon May 22 10:19:57 2000



From: Holt, Scott :      Scott.Holt-at-buehler.com
Date: Mon, 22 May 2000 09:03:15 -0500
Subject: Final Polishing of Metals

Contents Retrieved from Microscopy Listserver Archives
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John,

As a metallographic equipment and consumables manufacturer, BUEHLER¨ offers
a number
of products which would work for your application. However, I would suggest
our
MASTERPREP* (Part No. 40-6377-032) product. This is a 0.05 micron alumina
suspension. What makes it unique is the fact that it is produced through
the seeded
gel process instead of by calcining. In the seeded gel process, the alumina
is
precipitated from a liquid phase. This results in a better controlled
particulate
size distribution, higher particulate density, and more consistent particle
geometry. We've found
this product to be superior to all of the other 0.05 micron alumina products
that we sell for
preparation of ductile metals.

I hope this helps. If you need further information, you can email me
privately, call me at the
numbers listed below, or contact our sales department for pricing.

Best regards,
Scott D. Holt
BUEHLER¨ , LTD.
PO Box 1
41 Waukegan Rd.
Lake Bluff, IL 60044
(847)295-4546
or (800) 323-9330
www.buehler.com



From daemon Mon May 22 10:19:58 2000



From: Linda Barthel :      barthel-at-umich.edu
Date: Mon, 22 May 2000 10:44:08 -0400 (EDT)
Subject: QuickTime Videos

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I am also trying to figure out how to make a QuickTime video from a series
of images. I am using a PC, and the images are from a Zeiss 510 Confocal,
in LSM format, which have been converted to Tiff. The number I am dealing
with is 64 images. Any help would be greatly appreciated
Linda Barthel
Research Associate II
Department of Cell and Developmental Biology
University of Michigan
barthel-at-umich.edu





From daemon Mon May 22 10:20:00 2000



From: Anaspec :      anaspec-at-icon.co.za
Date: Mon, 22 May 2000 16:48:59 +0200
Subject: Philips Xl30 ESEM applications workshop required

Contents Retrieved from Microscopy Listserver Archives
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Hi all

We have a client, here in South Africa, who has recently purchased a Philips
XL30 ESEM LaB6 with a cryo system, hot stage and CL detectors.
We would like to now run a workshop, here on this system in South Africa, on
the applications of ESEM.
This system is a national facility and would therefore like to introduce the
local EM users to the exciting world of ESEM.

We realise that there are a few friends of ours in Australia who would be
ideal, but then we have various contacts in England and the USA too. In this
way we feel we should get a chance at the best choice of getting a really
exciting workshop set up or possibly a series of workshops.
We expect to keep the delegates riveted for at least 4 days of workshop. The
idea of the workshop is to show as many applications for ESEM as possible.
Then to specialise in some of the biological areas, as this would be some of
the more regular users for this system.
Those who could assist should please contact us and we will pass your
information on to the client. Please indicate your availability, field of
interest and the, always important, costs involved.

Thanks for your time.

Luc Harmsen
Anaspec, South Africa
Technical support on microscopy.
Tel + 27 (0) 11 476 3455
Fax + 27 (0) 11 476 7290
anaspec-at-icon.co.za
www.anaspec.co.za

Remember, ICEM 15 will be in
2002, Durban, South Africa.
www.icem15.com



From daemon Mon May 22 10:20:01 2000



From: Jean Dille :      jdille-at-ulb.ac.be
Date: Mon, 22 May 2000 17:05:00 +0200
Subject: cross sections of layered materials

Contents Retrieved from Microscopy Listserver Archives
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Hello all,

We have a sample of 6 micrometers Ni-Zn-P electroless plated onto 1 mm
thick Al
and we would like to prepare cross sections for TEM examination.
It looks very difficult to use the tripod polishing technique for metals.So,we
are thinking at an electropolishing procedure with our double-jet Tenupol
apparatus.
This would be done on slices cutted from a sample initially thicked up to 3mm
by electroplating.But how to avoid differential material removal problems?
Which material for plating?Which electrolyte for electropolishing?Other
method?
Does anyone have suggestions for us?

Thanks in advance for your help.

Jean Dille

Materials Science and Electrochemistry
Free University of Brussels CP 194/3
Avenue F.Roosevelt 50
1050 BRUSSELS
BELGIUM
tel:32-2-6502723
fax:32-2-6502786
e-mail: jdille-at-ulb.ac.be




From daemon Tue May 23 00:28:44 2000



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Mon, 22 May 2000 09:38:16 -0600
Subject: RE: new developments in imaging systems?

Contents Retrieved from Microscopy Listserver Archives
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Yes, Jim, this is absolutely correct.

The results are determined by the largest source of noise, be it the
initial electron statistics or subsequent noise introduced by
conversions or recording techniques. In this case (dark field imaging),
the largest source for noise is probably the statistical nature of the
electron beam. The lower you go in exposure (i.e., the fewer electrons
you record per pixel), the higher the relative noise. And there is no
way to get around this either by film or digital camera. The only way to
improve the noise is to go to more electrons, i.e., longer exposure or
brighter beam.

If the sample is so sensitive that even a dark field exposure damages
the sample, there is probably not much you can do.

If the problem is drift, a digital system can help you: Instead of 1
exposure at, for example, 30 seconds, acquire 3 exposures at 10 seconds.
Each one of them will be very noisy, but one can add them to get the
same noise figure as a 30 sec exposure. And the 10 sec exposure of each
one cuts down on drift. All you need to do is to align the 3 images.

I used to take dark field images with a digital camera and (of course) I
loved it. It allowed me to acquire the images and immediately see them.
I did not have to wait hours to develop the negatives and then find out
the exposure was not long enough (or too long).

Michael

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: jim [mailto:jim-at-proscitech.com.au]
Sent: Sunday, May 21, 2000 2:38 AM
To: 'Sergey Ryazantsev'; Microscopy-at-sparc5.microscopy.com


I find what you are trying to do interesting, but there are things that
may
never work and this may be one.

We had it confirmed by Michael Bode that good digital system "see
"single
photons - so that is not likely to improve much. Film too registers
single
electrons. We were told that in digital 1 electron can expose one pixel.
On
film one electron initiates the exposure of a silver halide and
subsequent
electrons in a near identical location increase the size of the grain.

Low noise (cooled cameras) hopefully do not add their own noise, but
they
cannot make up for lack of information in the image.

My case (below) was that in high resolution TEM at least, less exposed
images
will be inferior because there are simply not enough electrons to
produce a
good image. A more sensitive digital system uses fewer electrons and so
makes
matters worse.

In dark field EM we are using the beam indirectly and such images too
suffer
particularly from electron noise. Here too the use of a digital system
would
only make this worse. Furthermore, the slower 4489 film would be better
than
SO-163.

If you don't like long exposures (4 to 8 seconds I found a practical
limit),
you cannot ignore the reality of electron noise and hope to correct that
with
yet fewer electrons, albeit processed in a "noise-free" digital system.
I think that the answer is more electrons, i.e. a field emission source
or a
slower digital camera.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Friday, May 19, 2000 3:04 PM, Sergey Ryazantsev
[SMTP:sryazant-at-ucla.edu]
wrote:
}
}
} Jim hello
}
} I was talking about very special EM case: dark-field TEM. At this
point we
} are talking about a few electrons per square angstrom, even less. In
high
} resolution EM crystallography people have deal with 0.6 e/A2. At such
} conditions, high sensitivity and linearity of the modern digital
cameras
} may be a plus. Talking about long exposures --what about drift? I
never
} had good pictures at x100K with exposure longer that a few seconds. I
} don't understand your point about noise. In case of digital camera
the
} noise is a function of camera. Current cameras has a very low level
of
} noise (they uses cooling, etc) and we have to pay for that
astronomical
} price. This is a life. I am not friendly with TEM cameras, but I do
know
} that in the light microscopy people count individual photons using CCD
} cameras. In TEM camera we have phosphorous screen as a source of
image and
} my questions to Mickhael were addressed mostly to the problem how
} effectively (and correctly) information is traveled thought that funny
} screen. The Mickhael's answer is that screen does not affect dynamic
range
} and sensitivity is much higher than on convention films. Am I
correct,
} Mickhaels?
}
} Sergey
}
} } Date: Thu, 18 May 2000 14:15:02 +1000
} } From: jim {jim-at-proscitech.com.au}
} } Subject: RE: new developments in imaging systems?
} } To: 'Sergey Ryazantsev' {sryazant-at-ucla.edu} ,
} } "Microscopy-at-sparc5.microscopy.com"
{Microscopy-at-sparc5.microscopy.com}
} } Reply-to: "jim-at-proscitech.com.au" {jim-at-proscitech.com.au}
} } Organization: ProSciTech
} } X-Mailer: Microsoft Internet E-mail/MAPI - 8.0.0.4211
} }
}
} -----------------------------------------------------------------------
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} } The Microscopy ListServer -- Sponsor: The Microscopy Society of
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} -----------------------------------------------------------------------



From daemon Tue May 23 00:28:45 2000



From: Schibler, Matthew :      MSchibler-at-mednet.ucla.edu
Date: Mon, 22 May 2000 09:36:41 -0700
Subject: Treated coverslips

Contents Retrieved from Microscopy Listserver Archives
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My thanks to all those who responded to my question about treating
coverslips to make cells stick to them better. I have used one or two of
these in the past, but my colleague wanted to try some different approaches.
Thanks again.


Matthew J. Schibler Ph.D.
UCLA Brain Research Institute
1524A Gonda (Goldschmied) Center
for Neuroscience and Genetics
Los Angeles, CA 90095-1761

(310) 825-9783
FAX (310) 206-5855
E-mail: mschibler-at-mednet.ucla.edu



From daemon Tue May 23 00:28:46 2000



From: Simon C. Watkins :      swatkins+-at-pitt.edu
Date: Mon, 22 May 2000 12:51:15 -0400
Subject: QuickTime Videos

Contents Retrieved from Microscopy Listserver Archives
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Linda, if you get quicktime pro (its an upgrade to regular quicktime, costs
about $30 from Apple) you can import a numbered series of tiffs directly
into quicktime. which then can be made into a movie
Simon


-----Original Message-----
} From: Linda Barthel [mailto:barthel-at-umich.edu]
Sent: Monday, May 22, 2000 10:44 AM
To: Microscopy listserver



I am also trying to figure out how to make a QuickTime video from a series
of images. I am using a PC, and the images are from a Zeiss 510 Confocal,
in LSM format, which have been converted to Tiff. The number I am dealing
with is 64 images. Any help would be greatly appreciated
Linda Barthel
Research Associate II
Department of Cell and Developmental Biology
University of Michigan
barthel-at-umich.edu







From daemon Tue May 23 00:28:45 2000



From: Greg Erdos :      gwe-at-biotech.ufl.edu
Date: Mon, 22 May 2000 12:55:22 -0400
Subject: old FF machine

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have a functional, but old, Balzers Freeze Fracture machine. It is
headed for the scrap pile unless someone out there would like to have
it. I will explore the possibilities if anyone shows an interest.

Greg
Gregory W. Erdos, Ph.D.
Assistant Director, Biotechnology Program
PO Box 110580
University of Florida
Gainesville, FL 32611

Ph. 352-392-1295 Fax: 352-846-0251



From daemon Tue May 23 00:28:46 2000



From: john david whitaker :      jwhitake-at-u.washington.edu
Date: Mon, 22 May 2000 09:57:51 -0700 (PDT)
Subject: RE: final polishing for metals (Cu, Ni, Fe, NiFe)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ed,

Thanks for the input. I was hesitant to use diamond abrasives
on my samples due to previous advice. I was told that due to
the hardness of diamond, it tends to embed itself in soft
metals such as copper, which results in "smearing" more than
polishing. Do you have any input regarding the smearing issue?

John

On Fri, 19 May 2000, Edward Hirsch wrote:

} John,
} The solutions that the colloidal silica are in have a ph of 8.5 to 9.8
} depending on the formulation. I suggest you try our 0.05 micron water based
} polycrystalline diamond suspension. Allied High Tech
} http://www.alliedhightech.com sells this product. I would also suggest our
} Final A cloth for this polishing step. If you would like samples of either
} of these products please let me know and I will be happy to send it to you.
}
} If you need further technical assistance please contact me off-line and I
} will be happy to help or you may contact our main office at (800)675-1118
} located in CA.
}
} I hope this helps.
} Ed
} Please note, I have a financial interest in providing you with these
} products and other sample preparation equipment and consumable items.
}
} *************************************************
} Edward A. Hirsch
} Product Application Specialist
} Allied High Tech Products
} 2376 East Pacifica Place
} Rancho Dominguez, CA 90220
} ph: (919) 846-9628
} vm:(800)675-1118 x245
} fx: (310)762-6808
} http://www.alliedhightech.com
}
} Equipment and Consumables for Metallurgical Sample Preparation
} *************************************************
}
} -----Original Message-----
} From: john david whitaker [mailto:jwhitake-at-u.washington.edu]
} Sent: Friday, May 19, 2000 4:01 PM
} To: Microscopy Lister Server
} Subject: final polishing for metals (Cu, Ni, Fe, NiFe)
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I'm looking for information regarding final polishing
} solutions for preparing metallic cross-section samples
} for TEM analysis.
}
} I've looked into colloidal silica
} suspensions such as Ludox and Syton, and am concerned
} that their alkilinity may chemically etch my samples.
} I'm analyzing different compositions of NiFe
} on copper substrates, so the specimens are prone to
} preferential etching of different phases.
}
} Does anyone have any experience with this?
}
} Any input would be appreciated.
}
} John
}
}
}
}



From daemon Tue May 23 00:28:57 2000



From: Kevin W. Eliceiri :      eliceiri-at-facstaff.wisc.edu
Date: Mon, 22 May 2000 12:04:44 -0500
Subject: Re: QuickTime Videos

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Our software, 4D turnaround will make quicktime movies out of image
stacks that are in biorad format, pics, or tiff. Please see our
website for more details and to download:
http://www.loci.wisc.edu/4d/native/4d.html

Currently we have only a mac version available, however we will be
releasing a java version next month that works cross platform.

Best regards,
kevin

Kevin W. Eliceiri
Project Director
Laboratory for Optical and Computational Instrumentation
http://www.loci.wisc.edu
159 Animal Sciences
1675 Observatory Dr.
Madison, WI 53706
608-263-6288 voice
608-265-4076 fax



From daemon Tue May 23 00:28:59 2000



From: Kuenzler Ruediger :      kuenzler-at-dibis.de
Date: Mon, 22 May 2000 19:15:17 +0200
Subject: Re: TEM: new developments in imaging systems?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Margaret,

A view days ago I found your message in my mailbox.
Regarding your interest about digital imaging systems I can provide you some
information on our imaging plate system for TEM.

DIBIS Imaging Plate Technology is adapted for TEMs from, FEI/Philips,
LEO/Zeiss, JEOL and HITACHI. The Imaging Plate (81 x 100 mm) is inserted
into the sheet film cameras of the various TEMs and is directly exposed by
electrons.
After that the imaging plate reader creates digital images directly from the
plates without involving chemical processing thus providing extraordinary
image quality with 3600 x 3200 pixel at 25 µm and 16 or 20 bit dynamic range
with true linearity.
The high pixel count supports printouts in real photographic quality, also
on larger formats as you are used to from photographic film.
One Instrument in your lab will serve all your TEMs with highest quality
digital imaging technology, no matter what type and manufacturer.
The high performance, resolution, sensitivity and dynamic range makes
Imaging Plate Technology first choice for life science and material sciences
imaging, low dose applications and high dynamic diffraction patterns.

So, DIBIS introduces MICRON Digital Imaging Plate Technology as the
alternative to overcome the limits of CCD technology for TEM.

For more details visit our homepage.


R. Kuenzler
----------------------------------
DIBIS
Digital Biomedical Imaging Systems AG
Gewerbestra§e 11; D-75217 Birkenfeld
Tel.: +49 (0)7082 940639
Fax : +49 (0)7082 940076
E-Mail: contact-at-dibis.de
E-Mail: kuenzler-at-dibis.de
Internet: http://www.dibis.de





Margaret Dienelt schrieb:

Hi,

}
} Year after year I hopefully gather information about digital imaging
} systems for TEMs (ours is a JEOL 100CX) , only to learn we have no
} money. This year it looks like it might really happen but I have not
} kept up with innovations in the field and am wondering the following:
}
} 1. Anything new in the last two years -- especially in terms of
} cameras? I'm most familiar with the Gatan and AMT systems but their
} web sites don't reflect much in the way of changes over a year ago.
} 2. With more and more microscopists finally getting their systems --
} I'd love to get feedback.
}
} Thanks,
} Margaret
}
} P.S. Would welcome contacts from vendors.
}
} --
} Margaret Dienelt
}
} Plant Pathologist
} Electron Microscopy Lab
}
} Floral and Nursery Plants Research Unit
} U.S. National Arboretum/Agricultural Research Service/USDA
}
} B. 010A, Rm. 238, BARC-W
} 10300 Baltimore Avenue
} Beltsville MD. 20705 USA
}
} (301) 504-6097
} Fax: (301) 504-5096

--





From daemon Tue May 23 00:29:35 2000



From: G. Macdonald :      glenmac-at-u.washington.edu
Date: Mon, 22 May 2000 11:10:11 -0700 (PDT)
Subject: RE: QuickTime Videos

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In addition to QuickTime Pro,

GraphicConverter will convert a folder of numbered tiffs into QT. Just
be aware that after choosing the folder with your tiffs, and clicking
on Convert, the next window has an easily overlooked choice at its top
to save the files as ONE movie. Forget this selection and you will
have converted your 300 tiffs into 300 moov files. GraphicConverter has
many options for compression and output size.

NIH Image can open a folder of numbered tiffs with the 'Open All' option.
Use the 'Windows to Stack' command then save as QT. Image will place your
tiffs into the stack in the order in which they were opened. So if your
filenames don't end with an incrementing number you could open them
manually in the desired order.

Regards,
Glen


Glen MacDonald
Research Scientist
Hearing Research Laboratories of the
Virginia Merrill Bloedel Hearing Research Center
Box 35-7923
University of Washington
Seattle, WA 98195-7923
(206) 616-4156
glenmac-at-u.washington.edu

*********************************************************************
C:} The box said "Requires Windows95 or better". So I bought a Macintosh.
*********************************************************************




From daemon Tue May 23 00:29:06 2000



From: White, Woody N :      Woody.N.White-at-mcdermott.com
Date: Mon, 22 May 2000 13:14:00 -0500
Subject: Re:video from single images?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am unsure what versions are available for the Mac, but I have made videos
using ULEAD video editing software for the PC. TIFF images can be imported
and
displayed for "N" number of frames. Special effects (like a wipe or fade)
can
be added between sets fo (still) frame sets.

Do beware this process can tax the resources of most PCs. If the video is
uncompressed, the data rate can be 10+ MB/sec. I use a Matrox video card
which has MJPEG hardware compression. At full VHS resolution and 15
frames/sec
(not 29.9) the data rate is below 2 MB/sec. With MJPEG, I can get about 10
minutes of "fair" quality video on one CD-R (650 MB). Mpeg compression can
make a smaller file, but I havent tried a compression program I like -
Lousy
quality - Jumps, etc. I haven't tried the MPEG program from Xing Tech.
which is
rated better thatn the sharewares I have tried.

Another issue is the conversion time using software mpeg convertors is that
10
minutes of MJPEG video to mpeg on my PC (350 MHz/256 Meg Ram) takes well
over an
hour.

Woody White



From daemon Tue May 23 00:29:36 2000



From: Tom Isabell :      tc_isabell-at-fischione.com
Date: Mon, 22 May 2000 14:17:59 -0400
Subject: Job Listing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


E.A. Fischione Instruments, Inc., a rapidly growing company specializing in the development and manufacture of TEM specimen preparation instrumentation, is seeking a highly motivated individual for the position of a Application Scientist.
The candidate's responsibilities will include:

* Obtain and prepare TEM specimens of customer's material for sales
purposes.
* Analyze customers' specimens in Fischione's TEM.
* Library research to support design and application work activities.
* Answer customers' questions regarding use of Fischione products.
* Instrument training (when required).
* Provide technical support at major tradeshows.
* Give product demonstrations.
* Collaborate with potential customers on experiments.
* Write applications notes.
* Give scientific presentations.
* Conduct short courses and workshops on specimen preparation and
other Fischione related technology.
* Write refereed journal articles for publication.
* Generate information for Website.
* Revise instrument instruction manuals.
* Evaluate emerging microscopy related technologies and market
opportunities.
* Convey market opportunities to Fischione management.
* Obtain input from customers on possible new products and on
improvements to existing products.
* Work with the product design team on new product developments.
* Provide design support from the microscopist's standpoint.
* Prototype and test both existing product improvements and new products.
* Procure needed instrumentation to get specimen preparation facility
running appropriately.
* Optimize Fischione's TEM laboratory.
* Work with architects to design new EM facility when building expansion
occurs.
* Travel approximately 10%-20%.

The candidate should have a Ph.D in Material Science, Engineering, or Physics with a specialization in Electron Microscopy.

Salary is commensurate with experience.

E.A. Fischione Instruments, Inc. is an equal opportunity employer. Please send your resume and salary requirements to:

Human Resources Director
E.A. Fischione Instruments, Inc.
9003 Corporate Circle Export, PA 15632
Phone (724) 325-5444 FAX (724) 325-5443
E-mail: info-at-fischione.com



From daemon Tue May 23 00:29:36 2000



From: Anda Cornea :      corneaan-at-ohsu.edu
Date: Mon, 22 May 2000 11:33:16 -0700
Subject: Axiocam

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello!

We are considering the purchase of an Axiocam and would appreciate comments from other users.

Thanks!

Anda Cornea, Ph.D.
Oregon Regional Primate Research Center
505 NW 185th Avenue
Beaverton, OR 97006
ph: (503) 690-5293
fax:(503) 690-5384




From daemon Tue May 23 00:29:34 2000



From: White, Woody N :      Woody.N.White-at-mcdermott.com
Date: Mon, 22 May 2000 13:36:00 -0500
Subject: Re[2]:BSE resolution

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Gary,

I have never used a sintillator type BSE detector, but the major differences
are
two fold.

Typically (though diodes are getting better all the time) the Robinson has a
better low energy BSE detection effiency. It follows that for the same
noise
level electronics, it would exhibit better sensitivity. The dynamic range
problem will still exist for very different Zs in the field of view. In the
middle and upper portions of the sensitivity range (low and lower), I would
not
expect much difference between them. ...Any Comments from users of both????

I like the 4 quad diodes since I can go differential mode for macro
topography
(like fracture surfaces) and show the gross features while suppressing the
fine
detail.

The Pt coating can have a profound negative effect on sensitivity if not
extremely thin. If given a choice, I would always use carbon for the best
BSE
sensitivity. ...If not hazardous, I would use Be, BUT!!!! For elements in
the
range of Al & Si, I prefer to use carbon and lower beam voltages to minimize
penetration, especially if they are films or very small features.

I once presented some data illustrating the BSE signal attenuation as a
function
of sputtered Au thickness. But that data would be hard to retrieve now.

Woody White
McDermott Technology





===================================================
I use a Robinson Model 6 which is specified at a Z contrast of 0.003
at } = 2KV. From your work with a 0.1Z detector, what would you say
is the qualitative effect that a higher Z contrast detector would offer?
I am especially interested in imaging Al/Si alloys and I typically
sputter coat them with Pt. I may try carbon one of these days to
see what the difference might be.

tnx,
gary g.



From daemon Tue May 23 00:29:37 2000



From: Carl Henderson :      chender-at-umich.edu
Date: Mon, 22 May 2000 15:55:43 -0400
Subject: Re: Sputter coating artifact?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Yes, heating in the sputter coater is likely the cause.

Confirm this by checking the coated samples under the optical
microscope before putting them in the SEM. This will eliminate from
consideration any artifacts caused by higher SEM vacuum and electron
beam damage.


At 2:56 AM -0400 5/22/00, lherault wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

======================================
Carl Henderson
Electron Microbeam Analysis Laboratory
University of Michigan
2501 C.C. Little Bldg.
425 E. University Ave.
Ann Arbor, MI 48109-1063 USA
(734) 936-1550 FAX (734) 763-4690
======================================



From daemon Tue May 23 00:29:40 2000



From: Philip Oshel :      peoshel-at-facstaff.wisc.edu
Date: Mon, 22 May 2000 16:30:51 -0500
Subject: Re: Sputter coating artifact?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Are you using a magnetron sputter coater? If so, then you should not
be getting melting, etc. But ...

For how long did you coat your samples? You might try coating for
short periods, with some rest in between -- say 30 sec, turn off
(maybe add some argon to help carry away any heat), coat, rest, etc.
If your samples are very sensitive, use 10 sec. coat times. Rest for
30 sec more or less (empirically). I've done Teflon fabrics this way,
using 90 sec. coat periods without problems, but if your samples are
thick, this would increase the problems.

Phil

} I've had to coat some PLGA polymer samples and we think the coater may be
} melting them. The samples have a rolled or beaded edge and large cracks. I
} have jpeg images if anyone wants me to send them for a look. Are we
} correct in our assessment or should we look elsewhere for the source of
} cracks, shrinkage, beading around edges. These artifacts, BTW do not appear
} when the uncoated samples are checked under a light microscope.
}
} Thanks in advance.
}
} Ron L
} lherault-at-bu.edu

--
}}}}}}}}}}}}}}}}}{{{{{{{{{{{{{{{{{
Philip Oshel
Supervisor, AMFSC and BBPIC
Dept. of Animal Health and Biomedical Sciences
University of Wisconsin
1656 Linden Drive
Madison, WI 53706-1581
voice: (608) 263-4162
fax: (608) 262-7420 (dept. fax)



From daemon Tue May 23 00:29:40 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 22 May 2000 14:35:24 -0700
Subject: Re: QuickTime Videos

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


How long of running time, approximately?
Do you want dissolve from image to image or simple image swap?
Any audio?

gary g.


At 07:44 AM 5/22/00, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Tue May 23 00:29:40 2000



From: David Henriks :      Henriks-at-CompuServe.COM
Date: Mon, 22 May 2000 17:56:26 -0400
Subject: Sputter coating artifact?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Ron:

If your samples are particularly heat sensitive, you should probably look
into an Ion Beam Sputtering System. The heating effects in such a system
are negligible. Of course, you have the added advantages of thinner, more
uniform coatings etc. also. We do make the IBS/e Ion Beam Sputter
Deposition and Etching System. If this is an infrequent application for
you, perhaps I can put you in touch with one of our local users who could
help you out.

Let me know if that would be helpful.

Best regards-

David
Writing at 2:45:39 PM on 05/22/2000

***************************************************************************
************************

David Henriks
Vice President TEL: 800-728-2233
(toll free in the USA)
South Bay Technology, Inc. +1-949-492-2600
1120 Via Callejon FAX: +1-949-492-1499
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

***************************************************************************
************************

} } } } } Please visit us at http://www.southbaytech.com { { { { {

Manufacturers of precision sample preparation equipment and supplies for
metallography, crystallography and electron microscopy.
Message text written by "lherault"
}
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


I've had to coat some PLGA polymer samples and we think the coater may be
melting them. The samples have a rolled or beaded edge and large cracks.
I
have jpeg images if anyone wants me to send them for a look. Are we
correct in our assessment or should we look elsewhere for the source of
cracks, shrinkage, beading around edges. These artifacts, BTW do not
appear
when the uncoated samples are checked under a light microscope.

Thanks in advance.

Ron L
lherault-at-bu.edu

{



From daemon Tue May 23 00:29:41 2000



From: Donald Lovett :      lovett-at-tcnj.edu
Date: Mon, 22 May 2000 17:59:18 -0400 (EDT)
Subject: Used items for sale

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




We are moving to a new building and need to get rid of some old equipment.
The following are available in New Jersey, USA. All items were fully
functional when last used, most have all accessories and owners manuals.
Any reasonable offer considered.

For sale:

-GW Electronics attachments to go on Hitachi HS-510: Dual magnification
unit, Graphics generator, and Homomorphic Processor

-LKB 8800 Ultrotome III (thermal advance, includes ALL accessories)

-Denton Critcal Point Dryer (CPD-1) With extra baskets, seals, and
coupling for CO2 tank

-Ames Lab-Tek cryostat (slow leak in refrigerant line)

-Miles Tissue Tek cryostat

-3 ea Sorval MT-1 Porter Bloom ultramicrotome
#15601 w/ pivoting telescopic mount, baseplate, B&L sterozoom
head, adjustable cold light source and light switch box

-Zeiss microspectrophotometer (MPM microscope photometer for
cytospectrophotometry) for Zeiss Photo-microscope, Ultraphot,
Standard Universal microscopes (includes light chopper, power
supply, monochromator, photometer head, photomultiplier housing,
indicator unit, and coupling for microscope head)

-Printz automatic print dryer model JET 260158 ferrotyper drum and canvas
belt in like-new condition)

-Kodak Ektamatic Model 214-K automatic print processor -Accessories for
AO/Reichert Microstar compound scope (available with or without
microscope): AO Expostar photomicrographic system (lens and
shutter, control unit model 1190, polaroid and 35 mm film backs),
AO verical illuminator for incident light fluorescence microscopy
(includes mercury lamp model 2054A, filter housing, AND
microscope), Camera lucida attanchment AO #1030

- 2 ea Omni-mixer homogenizer with stand model # 17105, 16,000 rpm, no
impellers -Lightnin Mixer Model F (no impellor)

-B&L Dynazoom microscope with integrated 4x5 (polaroid) and 35 mm camera
backs

**Items looking for a good home (shipping cost plus a little extra
for us to be able to say that we actually sold them):

-B&L # 42-63-89 projecting compound scope (used to do camera lucida)

-metal microslide mailers/holders by Thomas; holds 4 slides (approx 500
avaiable)

-stainless steel frames for holding individual sheets/plates of 3 1/4 x 4
EM film

--Arthur Thomas Co., paraffin for 50-52oc (33 lib pkg of 1/4 lb bars)


Dealers are welcomed to contact us. Perhaps we could give you some items
plus a cash allowance to purchase some used items that you may have that
we want.

Don
______________________________________________________________________
Donald L. Lovett e-mail: lovett-at-tcnj.edu
Assoc. Professor, Dept. of Biology voice: (609) 771-2876
P.O. Box 7718 fax: (609) 637-5118
The College of New Jersey
Ewing, NJ 08628-0718






From daemon Tue May 23 00:29:41 2000



From: Lesley Weston :      lesley-at-interchange.ubc.ca
Date: Mon, 22 May 2000 15:00:15 -0700 (PDT)
Subject: Re: QuickTime Videos

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


You can get NIH Image to do this and a great deal more. For PC's it's called
Scion Image, and it's available free from www.scioncorp.com. Hope this helps.

Lesley Weston.



On Mon, 22 May 2000, Kevin W. Eliceiri wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} }
} } I am also trying to figure out how to make a QuickTime video from a series
} } of images. I am using a PC, and the images are from a Zeiss 510 Confocal,
} } in LSM format, which have been converted to Tiff. The number I am dealing
} } with is 64 images. Any help would be greatly appreciated
} } Linda Barthel
} } Research Associate II
} } Department of Cell and Developmental Biology
} } University of Michigan
} } barthel-at-umich.edu
}
} Our software, 4D turnaround will make quicktime movies out of image
} stacks that are in biorad format, pics, or tiff. Please see our
} website for more details and to download:
} http://www.loci.wisc.edu/4d/native/4d.html
}
} Currently we have only a mac version available, however we will be
} releasing a java version next month that works cross platform.
}
} Best regards,
} kevin
}
} Kevin W. Eliceiri
} Project Director
} Laboratory for Optical and Computational Instrumentation
} http://www.loci.wisc.edu
} 159 Animal Sciences
} 1675 Observatory Dr.
} Madison, WI 53706
} 608-263-6288 voice
} 608-265-4076 fax
}
}
}



From daemon Tue May 23 00:29:50 2000



From: frieda lim :      barbaricyawp-at-worldnet.att.net
Date: Mon, 22 May 2000 18:40:26 -0400
Subject: Re: Query for microscopic organism video/filmfootage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


: Frieda Lim
} Barbaric Yawp
} 224 west 13th Street #5r
} NYC, NY 10011-7775
} 212.206.0683
} barbaricyawp-at-att.net
}
} Dear MSA Members
}
} It was suggested to me that this may be the perfect spot to list my query.
} I would appreciate your perusal of the below queries and am anxious to
hear from you.
}
} Sincerely,
} Frieda Lim
}
}
}
} 1st query:
}
} Dear MSA Members :
}
} I invite you to take a close look at our query. We are a film production
} company seeking existing moving microscopic organism film or video
footage.
} We will utilize this footage for our single purpose of making a feature
} film. The film we are creating is a microscopic fantasia. Zooming in on
} your microscopic world, we will spin a tale through imagery and music.
}
} We would appreciate your help with obtaining some footage directly or any
} referring leads. Please contact us at your convenience to discuss further
} details.
}
} Sincerely,
} Frieda Lim
}
}
}
}
} Elaborating 2nd Query:
}
} Dear MSA Members:
}
} I thank you for your prompt response to my query for microscopic
} microorganism footage. Our intended use of your existing footage is to
edit
} & generate a full feature film comprised solely of microscopic imagery and
} music with which we hope, eventually, will be distributed to the general
} public at large--large in fact by mesmerizing all ages, the young & the
} young of heart. Our microscopic fantasia will bring to life a single
} narrative, the classic myth of Cupid & Psyche.
}
} In our original query, we had cast a wide net being intentionally
} non-specific with regard to the kind of microlife we are seeking. At this
} stage, we have no restrictions as to what microworld would be best suited
} for our story. However, our intent is to portray our story within a
} scientific veritable reality. We don't want micro species colliding that
} would never interact in truth. Right now we are in our initial phase of
} investigative hunting & gathering-a casting call for microscopic actors.
We
} actually want to cast organisms as actual characters and to use others as
} metaphorical imagery. My suspicion is that you have already created
footage
} for your scientific research and discovered many a talented organism
} awaiting their big break. We further suspect that we will need to pool
} microscopy resources and see what footage offered is most varied in look &
} movements that will best fill the wide assortment of roles required.
Since
} we are approaching your world as layman, we would appreciate your
expertise
} in advising what microorganism microcosm might be most readily available.
}
} To note, this is a low budget project with big hopes and dreams. We
} believe our film can achieve success similar to that of "Microcosmos"-the
'
} 96 Cannes Film Festival jury prize winner. That movie was testament that
} science has mass marketable enthusiasm in the entertainment world.
} Comparatively, we hope to explode your frontier world onto the big screen
} and excite a wide audience.
}
} So, if you know of some microorganisms wanting to make it big, we are
} anxious to hear from you. Thank you.
}
} Sincerely,
} Frieda Lim





From daemon Tue May 23 00:30:05 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 22 May 2000 17:43:59 -0700
Subject: quicktime

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html





From daemon Tue May 23 00:30:05 2000



From: Mark West :      mwest-at-ifcsun1.ifisiol.unam.mx
Date: Mon, 22 May 2000 18:55:44 -0600 (Hora est‡ndar de MŽxico)
Subject: starch granule success

Contents Retrieved from Microscopy Listserver Archives
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Hi all,

Firstly a confession, that my taxonomy is badly in need of repairing - the
alga we have been working with is not a blue-green algae at all - it's
actually Polytomella, a eukaryote, and I think is a relative of
Chlamydomonas, but doesn't have a cell wall or photosynthetic apparatus.

Anyway, embedding in Spurr's seems to have done the trick as far as
stopping the starch granules dropping out. I've just been looking at
grid-fulls of cells full of starch granules, and they're all there! Many
thanks for the suggestions. I'll be back with more problems soon!

Mark






********************************************
Mark West,
Unidad de Microscopia Electronica,
(Electron Microscopy Unit)
Instituto de Fisiologia Celular,
Universidad Nacional Autonoma de Mexico,
04510 Mexico D.F.

tel (unidad/lab) *(525) 622 5610*
(casa/home) (525) 619 3020
Fax (525) 616 2282
********************************************



From daemon Tue May 23 01:07:35 2000



From: Orion Analytical :      orionanalytical-at-worldnet.att.net
Date: Mon, 22 May 2000 21:42:45 -0400
Subject: microscopy and authentication

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Margaret -

We've applied microscopic techniques (e.g., polarized light microscopy,
fluorescence microscopy, FTIR microspectroscopy, and SEM-EDS) and
non-microscopic techniques to more than 700 projects involving historic and
artistic works, from Egyptian antiquity to contemporary. Material evidence
of authenticity -- or more likely, inauthenticity -- has been or become an
objective of many of these studies.

Scientific investigation plays an important role in the authentication
process, often providing indirect or direct evidence of date, and an
objective basis by which to compare materials and techniques to works of
unquestioned authenticity. Given sampling limitations and the layered
structure of many art objects and decorative finishes, microscopy is ideally
and elegantly suited to their study of the former - that is, structure and
composition. We use a new infinity-corrected optical/FTIR microscopy
system, that we had custom-fitted for polarized light microscopy,
fluorescence microscopy, and FTIR microspectroscopy (narrow-band MCTA and
wide-band MCTB detectors).

Your article sounds very interesting -- please feel free to call.

Jamie

James Martin
Orion Analytical, LLC
Post Office Box 550
Williamstown, MA 01267
phone: 413-458-0233
e-mail: martin-at-orionanalytical.com
website: www.orionanalytical.com



------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Hello,

I am new to this list. I would like to know if anyone has information I
could use to write an article on the analytical chemistry techniques used to
authenticate art objects using light microscopes or any other type of
microscope. Also, if anyone has information on any of the other techniques
used to authenticate art objects such as x-ray diffraction, x-ray
fluorescence, accelerator mass spectrometry, that would be helpful too.

Sincerely,

Margaret M. Mitchell
Assistant Editor
AOAC INTERNATIONAL
301-924-7077 (tel)
301-924-7089 (fax)
mmitchell-at-aoac.org




From daemon Tue May 23 01:07:35 2000



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Mon, 22 May 2000 22:15:15 -0500
Subject: Sectioning Ni-Zn-P coatings

Contents Retrieved from Microscopy Listserver Archives
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-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Jean Dille wrote:
=====================================================
We have a sample of 6 micrometers Ni-Zn-P electroless plated onto 1 mm
thick Al and we would like to prepare cross sections for TEM examination.
It looks very difficult to use the tripod polishing technique for metals.So,
we
are thinking at an electropolishing procedure with our double-jet Tenupol
apparatus.

This would be done on slices cutted from a sample initially thicked up to
3mm
by electroplating.But how to avoid differential material removal problems?
Which material for plating?Which electrolyte for electropolishing?Other
method?

Does anyone have suggestions for us?

Thanks in advance for your help.
====================================================
If the aluminum substrate can be thinned down a bit more, then a coating of
this thickness should be able to be thin sectioned using diamond knife
ultramicrotomy. It is hard to predict whether better results will be
obtained embedded or unembedded (there is a tendency for the coating to
separate from the substrate). We usually find that such separation is less
likely to occur if the sample is embedded. However, structure within the
coating is less well preserved when embedded. Naturally our experience has
been with our own SPI-Pon™ 812 epoxy embedding resin and SPI diamond knives,
but I would expect that at least most of the other "Epon substitutes" (and
knives) would work just as well. With regard to the substrate separation,
the tendency for this to happen can be reduced by using a knife included
angle that is smaller rather than larger (we were successful with 45 deg.).
The use of larger included angles, at least in our experience, seemed to
produce a level of compression artifacts in the sections that we found
unacceptable.

In any case, we have found the diamond knife thin sectioning approach to
often times offer certain advantages over the alternatives for sample
preparation.


Disclaimer: SPI Supplies offers materials science diamond knives and also
our preferred embedding resin, SPI-Pon 812 Embedding System. Our Structure
Probe™ laboratory services division performs this kind of diamond knife thin
sectioning as a service for commercial clients.

Chuck

PS: Remember that we are trying to become 100% paperless and the only way
that we can manage this kind of correspondence, in a paperless environment,
is for our correspondents to always reply by way of "reply" on their
software so that the entire string of correspondence on that topic gets
returned to us, and the entire correspondence history can be kept in one
place.

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.spi.cc
########################
============================================




From daemon Tue May 23 01:07:35 2000



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Mon, 22 May 2000 22:44:44 -0500
Subject: PLGA coating

Contents Retrieved from Microscopy Listserver Archives
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-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Ronald J. L'Herault wrote:
====================================================================
I've had to coat some PLGA polymer samples and we think the coater may be
melting them. The samples have a rolled or beaded edge and large cracks. I
have jpeg images if anyone wants me to send them for a look. Are we
correct in our assessment or should we look elsewhere for the source of
cracks, shrinkage, beading around edges. These artifacts, BTW do not appear
when the uncoated samples are checked under a light microscope.
====================================================================
If by PLGA you mean polylactic glycolic acid, which in certain forms could
be a (dissolvable) surgical suture material, then the polymer could be quite
hygroscopic and if it has had time to absorb enough moisture, it could be
evolving moisture, and that could be the reason for your problems.

Some sputter coaters have a "test mode" which enables the user to
momentarily expose in a gentle way the polymer surface to the glow of the
plasma. If moisture is evolving, then there will be an immediate diminution
of the quality of the vacuum. When confronted with that kind of situation,
we go through the cycle of "test mode", then pump down, test mode, etc,
until hitting the test mode button results in no more deflection of the
vacuum. Ten or more cycles might be needed but in the end, the surface
moisture is removed.

Then you are ready to coat.

If you are not talking about polylactic glycolic acid, I apologize for
taking up the extra bandwidth.

I realize that not all sputter coaters have such a test mode, but that is
the perfect kind of example where such a "test mode" has its greatest value.

Disclaimer: SPI Supplies manufactures sputter coaters with "test modes".

Chuck

PS: Remember that we are trying to become 100% paperless and the only way
that we can manage this kind of correspondence, in a paperless environment,
is for our correspondents to always reply by way of "reply" on their
software so that the entire string of correspondence on that topic gets
returned to us, and the entire correspondence history can be kept in one
place.

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.spi.cc
########################
============================================










From daemon Tue May 23 01:44:47 2000



From: Orion Analytical :      orionanalytical-at-worldnet.att.net
Date: Tue, 23 May 2000 00:54:27 -0500
Subject: microscopy and authentication of art objects

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Margaret -

I've applied microscopic techniques (e.g., polarized light microscopy,
fluorescence microscopy, FTIR microspectroscopy, and SEM-EDS) and
non-microscopic techniques to more than 700 projects involving historic and
artistic works, from Egyptian antiquity to contemporary. Material evidence
of authenticity -- or more likely, inauthenticity -- has been or become an
objective of many of these studies.

Scientific investigation plays an important role in the authentication
process, often providing indirect or direct evidence of date, and an
objective basis by which to compare materials and techniques to works of
unquestioned authenticity. Given sampling limitations and the layered
structure of many art objects and decorative finishes, microscopy is ideally
and elegantly suited to their study of the former - that is, structure and
composition. We use a new infinity-corrected optical/FTIR microscopy
system, that we had custom-fitted for polarized light microscopy,
fluorescence microscopy, and FTIR microspectroscopy (narrow-band MCTA and
wide-band MCTB detectors).

Your article sounds very interesting -- please feel free to call.

Jamie

James Martin
Orion Analytical, LLC
Post Office Box 550
Williamstown, MA 01267
phone: 413-458-0233
e-mail: martin-at-orionanalytical.com
website: www.orionanalytical.com



------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Hello,

I am new to this list. I would like to know if anyone has information I
could use to write an article on the analytical chemistry techniques used to
authenticate art objects using light microscopes or any other type of
microscope. Also, if anyone has information on any of the other techniques
used to authenticate art objects such as x-ray diffraction, x-ray
fluorescence, accelerator mass spectrometry, that would be helpful too.

Sincerely,

Margaret M. Mitchell
Assistant Editor
AOAC INTERNATIONAL
301-924-7077 (tel)
301-924-7089 (fax)
mmitchell-at-aoac.org




From daemon Tue May 23 01:44:48 2000



From: evansnd-at-ornl.gov (Neal D. Evans)
Date: Tue, 23 May 2000 01:08:52 -0500
Subject: QuickTime Videos from digital images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Edgar,

When I purchased a copy of Adobe Photoshop 5.5, it came bundled with their
"ImageReady 2.0" This package can be used to create animated GIF images
from layered images, or the animation exported in QuickTime format.

Regards,

Neal




From daemon Tue May 23 01:44:47 2000



From: evansnd-at-ornl.gov (Neal D. Evans)
Date: Tue, 23 May 2000 01:09:20 -0500
Subject: QuickTime Videos from digital images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Edgar,

When I purchased a copy of Adobe Photoshop 5.5, it came bundled with their
"ImageReady 2.0" This package can be used to create animated GIF images
from layered images, or the animation exported in QuickTime format.

Regards,

Neal




From daemon Tue May 23 01:44:48 2000



From: Angela Klaus :      avklaus-at-amnh.org
Date: Tue, 23 May 2000 01:09:38 -0500
Subject: Re: QuickTime Videos

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Linda,

What I ended up doing in your situation was downloading Confocal Assistant,
and reconstructing the TIFF series using that. You first need to add a
BioRad header... the software and tricks can be found at:

http://www.cs.ubc.ca/spider/ladic/source.html

You can export the animation from CA as an .avi file. Then if you have
Image Ready (Photoshop 5.5), you can convert the .avi to .mov. (I think
Graphics Converter will also work) A roundabout way to get the job done,
but it works.

All the best,

Angela

} I am also trying to figure out how to make a QuickTime video from a series
} of images. I am using a PC, and the images are from a Zeiss 510 Confocal,
} in LSM format, which have been converted to Tiff. The number I am dealing
} with is 64 images. Any help would be greatly appreciated
} Linda Barthel
} Research Associate II
} Department of Cell and Developmental Biology
} University of Michigan
} barthel-at-umich.edu
}
}
}
}
}
}
---------------------------------------------
Angela V. Klaus

Laboratory Manager, Interdepartmental Laboratories
American Museum of Natural History
Central Park West at 79th Street
New York, NY 10024-5192 USA

Email: avklaus-at-amnh.org
Tel: 212-769-5977, 5469
Fax: 212-496-3480
---------------------------------------------




From daemon Tue May 23 05:15:12 2000



From: HARRISm-at-esm-semi.co.uk
Date: Tue, 23 May 2000 10:02 +0000 (GMT)
Subject: Equipment reliability

Contents Retrieved from Microscopy Listserver Archives
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A how long is a piece of string ? type question


I would be pleased if anyone could broaden my views on equipment
reliability .
Basically we have a 3yr old FESEM which I consider to be fairly
reliable in that it is on call 24hrs/day , has numerous ( non
dedicated users ) and apart from downtime for filament change and the
odd wear and tear type problems answers our needs .
As we do not have a back up instrument and when we do experience
problems it's always at the worse time certain personnel have the
impression that it is unreliable .

What do other sem users expect in terms of reliability , apart from my
' subjective ' comments is it quantifiable , would approx 2wks /year
downtime including planned maintenance be considered excessive ?

Regards
Martyn Harris
harrism-at-esm-semi.co.uk



From daemon Tue May 23 08:08:13 2000



From: Geoff Williams :      Geoffrey.Lloyd.Williams-at-cmich.edu
Date: Tue, 23 May 2000 08:12:56 -0400
Subject: Re: video from single images?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have done this procedure many times using many different programs.
Most often I was using TIFF files in sequence. I can't remember all the
names
of the programs that I tried, most were freeware or shareware including
NIH
image and the results were never to my satisfaction, I wanted control
over
Frames per second, compression, output format, light levels, you know
complete
control.
The program that I wound up using was Adobe After Effects. An
incredible
program, capabilities way beyond what was needed for creating simple
sequence
movies from 3D volumes captured in a LSCM and rendered on a SGI. The
benefit is
unlimited file type importation (well nearly unlimited - hey it supports
SGI
.rgb files!) and complete control over the creation process. I could
make an
animated gif with any frame rate I wanted or a full compression-less
Quicktime
(.mov) or Microsoft Video for Windows format (.avi) with sound and
special
effects.

Most of you have used Adobe photoshop at one time or another, Adobe
After
Effects has the same intuitive interface and many of the same filters
and image
adjusting features. The educational discount for the lab brought the
price down
to less the $300! An incredible value for anyone doing lots of routine
slice
parade movies or animations for presentations.

If you have the ability to record sound on your computer you can even
record a
whole seminar presentation. Imagine showing up at a meeting plugging in
your
laptop computer (with sound) to the projection system and clicking
play. You
can sit down and listen to your own presentation, and then answer
questions at
the end. No more forgetting to mention a point, you can make sure that
you are
making sense. Of course this all takes away from the art of the
presentation.
I have never done this mind you all but the idea is intriguing isn't it?

Adobe also has a software program called Premier that is used in the
film
industry to do even more digital effects and post production (I think
the last
guess I heard was that about 75% of all rolling credits and film intros
are done
with this program and many of the same features are in After Effects).

-Geoff

--
Geoff Williams, M.S.

Electron Microscope Facility Supervisor
Biology Department
Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859

Geoffrey.Lloyd.Williams-at-cmich.edu
517 774-3576
517 774-3462 (fax)




From daemon Tue May 23 08:08:12 2000



From: Ken Converse :      qualityimages-at-netrax.net
Date: Tue, 23 May 2000 07:18:40 -0700
Subject: Re: TEM: Help

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Gordon Couger wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} } From: "Tina Carvalho" {tina-at-pbrc.hawaii.edu}
} } On Thu, 18 May 2000 ComCryLab1-at-aol.com-at-sparc5.microscopy.com wrote:
} }
} } } Dear Friends,
} } } Can anyone give me advice regarding the dismantling and
} } } the packing for shipment of a Philips 201 TEM? I need to
} } } pay particular attention to not disturbing the alignment.
} } } I plan to move the TEM from Univ. of Delaware to Naples, Florida
} } } in an enclosed U-Haul Trailer. Any help or suggestions
} } } will be most welcome.
} }
} } I will make sure Ron Veil, a very experienced independent EM service tech,
} } sees this and has a chance to reply. It was with his advice that we (my
} } hubby and I and its new owner) packed up and shipped a Philips 201. Like
} } you, we wanted to ship it intact, column on and all. We built a heavy
} } duty skid and added a strong base to which we bolted the instrument. Then
} } we wrapped it with whatever that plastic packing tape that is like Saran
} } Wrap is called, making sure to secure any part of the column we didn't
} } want to move, but avoiding putting any pressure on things, such as the
} } aperture drives. Wrap the bottom, wrap the column, wrap the column to the
} } bottom and back again, etc. Build a crate up around the instrument.
} } I think I remember putting a wood insert with a crescent cut out near the
} } top of, but not touching the column, but hubby thinks not. The idea is
} } NOT to let any shock to the crate get transferred to the column, so
} } perhaps we didn't. Add some packing material, such as old egg crate foam
} } from your bed (it needed replacing anyway) and whatever. Then, and this
} } was the fun part, buy that stuff that when you mix it with a catalyst,
} } produces huge volumes of foam that hardens in a few minutes. I think you
} } can also buy spray cans of similar stuff, but since hubby had this on hand
} } for other things, we had the bulk stuff. Fill the voids in the crate with
} } it. It will easily chip off later. Add a top, and away you go. The
} } scope made it in great shape.
} }
} } I think we packed the rotary pump and a couple of other things
} } separately. Get it into the truck and TIE IT DOWN. I say this because an
} } SEM we once packed for shipping with the base and saran, but no closed
} } crate, made it from Hawaii to Ohio, then slid in the shippers truck in the
} } last mile and slammed into the driver's seat. The driver was OK, the ion
} } getter pump was bent, and the column needed a bit of work, but it could
} } have been worse. Duh.
} }
} } I've also packed up a couple of Denton vcuum evaporators and a couple of
} } ultramicrotomes. The saran stuff and blow foam are a good way to
} } stabilize the instruments.
} }
} Since you are using a U haul you don't have to worry about sides
} and a top on the crate. Make sure it is tied down real well. Dry
} wall screws through the crate and into the floor work well for
} locking it down but make sure you have it braced with timbers
} to the front and sides of the trailer in case you make a panic
} stop.
}
} Also make sure that the weight is centers in front of the trailer
} axle if you use a trailer. Negitive weight on the trailer tounge
} at the very least makes driving very interesting. You have no
} Idea how fast a trailer can pass you while it is still tied on to the
} truck. I have had the privilege of experiencing this and I can promise
} you that you won't enjoy it:).
}
} The foam in place stuff is great. Just cover the instrument in plastic
} and foam away. If you can get foam between the instrument and the
} Support the foam will dissipate most of those little shocks that get
} things out of line.
}
} Last of all make sure you understand what you insurance covers and
} what it doesn't on moving equipment. You should be fine on liability but
} the value of the contents is probably not covered. Rental truck companies
} have contents insurance as an extra.
}
} Good luck
} Gordon
}
} Gordon Couger gcouger-at-couger.com
} Stillwater, OK www.couger.com/gcouger
} 405 624-2855 GMT -6:00


Gordon,
Now I guess I know who to blame when I get U-Haul trailers that are in
p----poor shape to move SEMs. Where do you get off running screws
through the floor of a trailer you don't own?

Unbelievable!

Ken Converse
owner
Quality Images
Delta, PA



From daemon Fri May 26 11:55:53 2000



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Fri, 26 May 2000 08:13:37 -0600
Subject: RE: new developments in imaging systems?

Contents Retrieved from Microscopy Listserver Archives
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You could probably expose your CCD chip directly to the electron bean --
and buy a new chip every few hundred exposures or so! Electrons have
mass and carry momentum, which gets transformed into force as they are
stopped in the crystal, especially at higher keV. This can lead to
damage. Photons only heat up the target.

As far as I know ALL TEM cameras use a medium to convert electrons into
light, and this light is then detected. The medium is either a phosphor
screen or a very thin YAG crystal. From there on it is different. Some
cameras use a fiber-optic to guide the photons to the chip, others use
mirrors and lenses. So, it is not simply a matter of comparing the QE of
the chip, the entire system has to taken into account. Also, each
electron creates many photons in the phosphor or YAG, and in principle
only one photon is enough to create a signal in the CCD. In other words,
a CCD could theoretically detect "fractions" of an electron, whereas the
film is directly exposed to the electron. Of course the electron can
"rattle" around in the film and activate several grains, but as you can
see, the question of comparing sensitivities now becomes one of
comparing two different physical processes. It can definitely be done,
but it's not easy.

Michael

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: hpadams [mailto:hpadams-at-bcm.tmc.edu]
Sent: Thursday, May 25, 2000 12:30 PM
To: microscopy-at-sparc5.microscopy.com


I have been following off and on this discussion on CCD cameras for EM.
Much of this discussion has been concerned with "sensitivity". I am
being
naive here, but when we talk about "sensitivity" of CCDs, isn't this the
same
thing as the QE of camera/chip? I don't think I have ever seen a QE for
em
CCD's for various accelerating voltages/wavelenghts. Does the electron
beam
directly hit the silicon photodyodes or it there an interface that
converts
the incoming electrons to different (longer?) wavelengts? I know em
films are
sensitive to specific acc voltages. Are CCD cameras for em the same. For
long
exposures it may not matter, but for short exposures or low level
intensity
does the QE of the camera come into play?

} ===== Original Message From Jenichen {Jenichen-at-proscan.de} =====
} -----------------------------------------------------------------------
-
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Fri May 26 11:55:53 2000



From: Tracey M. Pepper :      tpepper-at-iastate.edu
Date: Fri, 26 May 2000 09:40:16 -0500
Subject: cathodoluminescence?

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Hello all,
I have a researcher that is interested in utilizing florescent probes to
label material with the desire to visualize with a 50 angstrom
resolution. Are there any service facilities out there that have a
cathodoluminescence detector on their SEM or STEM that would be able to
assist him (via contract)?
Thank you!


Tracey Pepper
Supervisor
Bessey Microscopy Facility
Iowa State University
ph: 515-294-3872
fax: 515.294.1337



From daemon Fri May 26 11:55:54 2000



From: Paul Voyles :      voyles-at-research.nj.nec.com
Date: Fri, 26 May 2000 11:11:23 -0500
Subject: RE: new developments in imaging systems?

Contents Retrieved from Microscopy Listserver Archives
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} You could probably expose your CCD chip directly to the electron bean --
} and buy a new chip every few hundred exposures or so! Electrons have

There is an even worse problem with exposing the CCD directly the
electron beam. The p-n junctions in the CCD chip have a certain
"well capacity" - a number of electron/hole pairs they can hold
before they saturate. Fast (keV) electrons are much more efficient
at producing electron/hole pairs than photons - so much so that a
pixel on the CCD would saturate after about 15 fast electrons hit it.
Just the square root N shot noise at that level is about 25% - larger
than typical TEM micrograph contrast of 10-20%. That's why a
phosphor or scintillator is necessary to transform the fast electrons
into photons.

In case anyone is interested, you can learn more than you ever
possibly want to know about CCD cameras in:

"Applications of slow-scan CCD cameras in transmission electron
microscopy" O. L. Krivanek and P. E. Mooney, Ultramicroscopy V. 49 p.
95-108 (1993). First description of Gatan CCD cameras, including
some design issues, and measurements of the linearity, modulation
transfer function (MTF), and detector quantum efficiency (DQE).

"Methods to measure the properties of slow-scan CCD cameras for
electron detection" W. J. de Ruijter and J. K. Weiss, Rev. Sci.
Instrum. V. 63, p. 4314 - 4321 (1992). Another early paper, includes
comparisons to photographic plates. Uses a slightly different
definition of the MTF from everyone else.

J. M. Zuo "Electron detection characteristics of slow-scan CCD
camera", Ultramicroscopy V. 66 p. 21-33, (1996). Describes gain,
MTF, and DQE measurements, measurement techniques based on stochastic
noise (blank beam) images, and theory. This is a good place to start.

"Quantitative characterization of point spread function and
detection quantum efficiency for a YAG scintillator slow scan CCD
camera" A. L. Weickenmeier, W. Nuchter, and J. Mayer, Optik, V. 99,
p. 147-154, (1995). Line-scan method of measuring the MTF.


Happy reading,
Paul




Paul Voyles, voyles-at-research.nj.nec.com
voice: (609) 951-2627, fax: (609) 951-2496
NEC Research Institute
4 Independence Way
Princeton, NJ 08540 USA
{http://www.neci.nj.nec.com/homepages/voyles/fluct.html}



From daemon Fri May 26 11:55:56 2000



From: White, Woody N :      Woody.N.White-at-mcdermott.com
Date: Fri, 26 May 2000 07:49:00 -0500
Subject: RE: BSE Information

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Victor,

On which systems have you observed higher focused beam current from the
false
(first) saturation peak rather than "true" saturation? I have not observed
this
in 18 years with an Etec nor on the new Hitachi.

Woody White

-----------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Hi friends
I agree Steve, sometimes (I seem it depends on electron gun design and
distance between wenelt and anode, wenelt and filament) the first peak
on saturation curve is more than saturation level even in secondary
emission signal.
Victor Sidorenko, ANTRON Co.Ltd., Moscow, Russia


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---
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America



From daemon Fri May 26 11:55:56 2000



From: White, Woody N :      Woody.N.White-at-mcdermott.com
Date: Fri, 26 May 2000 07:42:00 -0500
Subject: RE: BSE first sat peak

Contents Retrieved from Microscopy Listserver Archives
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I would also be interested in learning how/why. I understand that if the
larger
spot size delivers more incident beam current, signal to noise is improved,
but
this does not seem to be the parameter to which Steve is referring.

Woody White
McDermott Technology

-----------------------------------

The Microscopy ListServer -- Sponsor: The Microscopy Society of America


At 05:07 PM 25-05-2000 +0100, you wrote:
Steve Chapman wrote,
SNIP
, then by de-saturating the larger source
} will result in a larger probe dimension on the specimen; increasing the
} probe diameter increases the volume of material involved in the production
} of BSE.
}
Ken Wrote:
But how does this lead to an increase in the BSE differentiation (implying
more signal and hence less noise). Surely just defocussing would do the same
thing. Defocussing as such should not effect the BSE coeffecient, unless you
had sub surface charging or some other artefact.

Very interesting!
Ken.



From daemon Fri May 26 11:55:57 2000



From: COURYHOUSE-at-aol.com
Date: Fri, 26 May 2000 12:10:30 EDT
Subject: Re: Service Manuals

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Dear Walter, yes we have started one. We continue to gather as time goes on
many of them are stored at the moment as the library section that will house
them will not be finished until November. we are planning to offer some of
them online in pdf format when we have permission from the various
companies on the really old ones that they seem not to care about.

We need anything we can get our hands on and also include in this pursuit
their older equipment brochures and catalogs. Any assistance of originals or
good Xerox copies is great!

thanks Ed Sharpe archivist for SMECC

{ { Subj: Service Manuals
Date: 5/25/00 10:54:00 PM US Mountain Standard Time
From: Corvos-at-aol.com-at-sparc5.microscopy.com
To: microscopy-at-sparc5.microscopy.com

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America
To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
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All,

I would like to know if any has started an extensive collection of
Microscopy
Service Manuals?

Regards,

Walter Protheroe
E-MAC, Inc.
} }



From daemon Fri May 26 11:55:57 2000



From: rnatarajan :      rnataraj-at-hd2.dot.net.in
Date: Fri, 26 May 2000 22:20:17 +0530 (IST)
Subject: Accomodation at M&M 2000

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Dear fellow researcher,

I am an author and platform presenter at the M&M 2000 conference at Philadelphia in August,2000. I am a scientist at the National Geophysical Research Insitute , India. I am looking for living accomodation during my 5 day stay at Philadelphia. I was wondering if any of you will be able to provide me with free accomodation or would be aware of any other resources who may be able to help me in this regard. Due to the limited fiancial resources avaliable to me from my lab, I am looking forward to help from my fellow researchers. Hope you can help me in this regard. I am eagerly looking forward to contributing to the M&M 2000 conference.

With kind regards
R. Natarajan
Scientist, National Geophysical Research Institute, Hyderabad,
India
e-mail: rnataraj-at-hd2.dot.net.in
Phone: 91-40-7170141 X 2430 (W)
Fax: 91-40-7170564



From daemon Fri May 26 17:02:34 2000



From: Joseph Passero :      jp-at-spacelab.net
Date: Fri, 26 May 2000 13:25:37 -0400
Subject: Light Microscope: Looking For a Leitz....

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I am looking for a 170mm Leitz body tube lens (a field lens as Leitz called it) for a Leitz
Ortholux.

This is the lens that is inside the nose turret.

The engraving on the lens is 170/223, 1.25 W

If you have any older Leitz items to sell please let me know.

Thank You

Best Regards

Joseph Passero

mailto:jp-at-spacelab.net



________________________________________________________
1stUp.com - Free the Web
Get your free Internet access at http://www.1stUp.com



From daemon Fri May 26 17:02:34 2000



From: Kim DeRuyter :      fnksd1-at-uaf.edu
Date: Fri, 26 May 2000 10:02:27 -0800
Subject: Thanks for Varian and ISI help

Contents Retrieved from Microscopy Listserver Archives
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A hearty thank you to everyone who replied to my request for help. I had
sources for everything I needed before the evening was out. It is the
generosity of the subscribers that makes this list-serve a great
resource. Thanks again.

Kim DeRuyter
Electron Microscopy Technician
308 Natural Science Facility
P.O. Box 755780
University of Alaska
Fairbanks, AK 99775-5780
907-474-5452
907-474-5163 fax



From daemon Sat May 27 12:37:36 2000



From: rnatarajan :      rnataraj-at-hd2.dot.net.in
Date: Sat, 27 May 2000 07:54:59 +0530 (IST)
Subject: Accomodation at M&M 2000

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear fellow researcher,

I am an author and platform presenter at the M&M 2000 conference at Philadelphia in August,2000. I am a male scientist at the National Geophysical Research Insitute , India. I am looking for living accomodation during my 5 day stay at Philadelphia. I was wondering if any of you will be able to provide me with free accomodation or would be aware of any other resources who may be able to help me in this regard. Due to the limited fiancial resources avaliable to me from my lab, I am looking forward to help from my fellow researchers. Hope you can help me in this regard. I am eagerly looking forward to contributing to the M&M 2000 conference.

With kind regards
Mr. R. Natarajan
Scientist, National Geophysical Research Institute, Hyderabad,
India
e-mail: rnataraj-at-hd2.dot.net.in
Phone: 91-40-7170141 X 2430 (W)
Fax: 91-40-7170564



From daemon Sat May 27 12:57:55 2000



From: Tony Kowal :      askowal-at-midway.uchicago.edu
Date: Sat, 27 May 2000 12:38:36 -0500
Subject: Tissue Drier

Contents Retrieved from Microscopy Listserver Archives
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Dear Fellow Microscopists -
Someone in the lab in which I work wants to do SEM of yeast colonies. I
was able to locate some references and a general protocol, which seems
rather straight forward. However, the authors two of the papers refer to
an "Edwards Pearce Tissue Drier, EPD3". I contacted Edwards to find out
what this tissue drier is, however they said they could not give me any
info because it is no longer manufactured.
I am thinking that this tissue drier is simply a freeze drier, but I am not
sure. Does anyone out there have any insight into what this thing is or
what it does? Thanks for your help!!

Tony


Tony Kowal
Research Assistant
for
Dr. Susan Lindquist

Howard Hughes Medical Institute
The University of Chicago
5841 S. Maryland
MC 1028, Room N339
Chicago, IL 60637

Phone: (773) 702-8795
Fax: (773)702-7254
e-mail: askowal-at-midway.uchicago.edu
Pager: on campus - 188 - 9668 (YNOT)
off campus - (773)753-1880 - 9668




From daemon Sun May 28 08:28:16 2000



From: Pbgrover-at-aol.com
Date: Sat, 27 May 2000 16:19:18 EDT
Subject: charred plant seeds

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Esteemed colleagues,

A student has asked me if I can find a way to tell whether small plant seeds
have been charred or not. She is examining seeds from an anthropological dig
on a site probably inhabited both in historic times (170 years ago) and
prehistoric (1000+ years ago). It seems that for seeds to date from the
earlier inhabitation, they would need to be charred, which apparently
preserves them.

I looked at some (uncoated) in the SEM, along with some 'fresh' seeds of same
species as controls. The seeds of unknown age showed a lot more charging
than the fresh ones. Could this be evidence that they are uncharred (i.e.,
would charring make them more conductive/better secondary electron
producers?) Does anyone know of a method of differentiating between charred
& uncharred seeds?

Thank you thank you thank you :0)

Paul Grover
Chief Microscopist and Bottle Washer
Microvista Laboratory
Lafayette, IN



From daemon Sun May 28 08:38:23 2000



From: BENJAMIN HIDALGO :      bhidalgo-at-ci.udo.edu.ve
Date: Sun, 28 May 2000 08:29:20 -0500
Subject: I NEED SOME INFO

Contents Retrieved from Microscopy Listserver Archives
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Hello anybody from the ListServer: I need to have information on the
approximate market price of a HITACHI S-800 FE SEM. Thank you.
Ben Hidalgo-Prada




From daemon Sun May 28 15:07:28 2000



From: Jonathan Barnard :      Jonathan.Barnard-at-angstrom.uu.se
Date: Mon, 29 May 2000 08:44:21 +0200
Subject: Sample prep: ceramics

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The Edwards Pearce Tissue drier was a freeze drier with a small
peltier cooled specimen stage. The peltier device was a water-
cooled 3-stage stack, achieving about -60oC on a stage about the
size of a 35mm negative. There was provision for a small tray of
phosphorus pentoxide, and the specimen chamber was a simple
Pyrex glass bell, pumped by a rotary pump.

Date sent: Sat, 27 May 2000 12:38:36 -0500
To: Microscopy-at-sparc5.microscopy.com
} From: Tony Kowal {askowal-at-midway.uchicago.edu}


How about using the low angle cleaving method of Rafferty? Polish with the
SiC close to say the {110} planes (e.g. ten degrees) and then cleave along
both the scratches and the {110} plane to get a thin acute wedge. It will
take some practice, but should work generally with most Perovskites.

references:
1/Thin Solid Films Vol308-309 (1997) pp399-405
S.D.Walck & J.P.McCaffrey: The small angle cleavage technique applied to
coatings and thin films

2/Thin Solid Films Vol304 (1997) pp157-159
Suli Suder, C.A.Faunce & S.E.Donelly: Thin solid film preparation by a
small-angle cleavage for transmission electron microscopy

-I hope thses can provide some help. By the way this is not a definitive
list, but I am sure Scott Walck can give you a far greater insight to this
method.

Regards, Jon

********************************************************
Dr Jonathan Barnard

Analytical Materials Physics
The Angstrom Laboratory, Uppsala University
P O Box 534, SE-751 21 Uppsala, Sweden
Phone: +46-(0)18-4716838 Fax: +46-(0)18-500131
Phone: Microscope room +46 18 471 6365
http://www.angstrom.uu.se/analytical/home.html
********************************************************



From daemon Mon May 29 05:50:02 2000



From: Chris Jeffree :      cjeffree-at-srv0.bio.ed.ac.uk
Date: Mon, 29 May 2000 11:33:16 +0000
Subject: Re: Tissue Drier

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Chuck
CPD certainly did become the most popular method by a country
mile for SEM specimen drying, but freeze-drying still has
advantages over it in some situations. These include, for example,
specimens where lipid content or lipid structures must be retained
(e.g. plant and insect epicuticles), where the specimen is an
aggregate of objects loosely bound by a fluid or a mucilaginous
matrix (e.g. it could be an advantage in Tony Kowal's yeast and
bacterial colonies, soils and clays), or where the specimen is
mechanically fragile and the components would be dispersed on
submersion in baths of liquid during fixation and solvent drying and
CPD (soils & clays, fungal sporangia, yeast and bacterial colonies).

The down side of freeze-drying in most of these contexts is that
some shrinkage and distortion almost always results.
Consequently, for almost all the situations listed above, and a host
of others as well, Low-temperature SEM became the method of
choice. In LTSEM the specimen can be viewed fully frozen-
hydrated, but most commercially-available LTSEM systems have
specimen temperature control, and full or partial freeze-drying can
be undertaken either on the SEM specimen stage or in the cryo-
preparation unit if required.

Anyone seeking a freeze-drier unit for EM specimens should
contact Emitech who make a peltier-cooled unit (K750) which
operates around -60oC (and is not unlike the Edwards-Pearce
tissue drier) and a turbo molecular pumped Liquid nitrogen cooled
low-temperature freeze drier (K775) which operates {-80oC.

http://www.Emitech.co.uk/

I have no financial interest in this company

Chris


} Hi Chris,
}
} Am I not remembering correctly, or was this earlier approach sort of a
} precursor to the critical point drying technique? I mean, did not people use
} this earlier technique because that was all they knew and then when the
} option of CPD came along, everyone switched over to it?
}
} I myself was reluctant to say that because that was a very long time ago and
} the memory does dull at the edges.
}
} Chuck
} -------- REPLY, Original message follows --------
}
} } Date: Sunday, 28-May-00 08:57 PM
} }
} } From: Chris Jeffree \ Internet: (cjeffree-at-srv0.bio.ed.ac.uk)
} } To: Tony Kowal \ Internet: (askowal-at-midway.uchicago.edu
} )
} } cc: MICROSCOPY BB \ Internet:
} } (microscopy-at-sparc5.microscopy.com)
} }
} } Subject: Re: Tissue Drier
} }
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To
} } Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com On-
} Line
} } Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } The Edwards Pearce Tissue drier was a freeze drier with a small peltier
} cooled
} } specimen stage. The peltier device was a water- cooled 3-stage stack,
} achieving
} } about -60oC on a stage about the size of a 35mm negative. There was
} provision
} } for a small tray of phosphorus pentoxide, and the specimen chamber was a
} } simple Pyrex glass bell, pumped by a rotary pump.
} }
} } Date sent: Sat, 27 May 2000 12:38:36 -0500
} } To: Microscopy-at-sparc5.microscopy.com
} } } From: Tony Kowal {askowal-at-midway.uchicago.edu}
} } Subject: Tissue Drier
} }
} } } ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } -----------------------------------------------------------------------.
} } }
} } }
} } } Dear Fellow Microscopists -
} } } Someone in the lab in which I work wants to do SEM of yeast colonies. I
} } } was able to locate some references and a general protocol, which seems
} } } rather straight forward. However, the authors two of the papers refer
} to
} } } an "Edwards Pearce Tissue Drier, EPD3". I contacted Edwards to find out
} } } what this tissue drier is, however they said they could not give me any
} } } info because it is no longer manufactured.
} } } I am thinking that this tissue drier is simply a freeze drier, but I am
} not
} } } sure. Does anyone out there have any insight into what this thing is or
} } } what it does? Thanks for your help!!
} } }
} } } Tony
} } }
} } }
} } } Tony Kowal
} } } Research Assistant
} } } for
} } } Dr. Susan Lindquist
} } }
} } } Howard Hughes Medical Institute
} } } The University of Chicago
} } } 5841 S. Maryland
} } } MC 1028, Room N339
} } } Chicago, IL 60637
} } }
} } } Phone: (773) 702-8795
} } } Fax: (773)702-7254
} } } e-mail: askowal-at-midway.uchicago.edu
} } } Pager: on campus - 188 - 9668 (YNOT)
} } } off campus - (773)753-1880 - 9668
} } }
} } }
} } }
} }
} }
} } ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
} } Dr Chris Jeffree
} } University of Edinburgh
} } Biological Sciences EM Facility
} } Daniel Rutherford Building
} } King's Buildings EDINBURGH EH9 3JH
} } Tel: +44 (0) 131 650 5345
} } FAX: +44 (0) 131 650 6563
} }
} } Inveresk Cottage, 26 Carberry Road,
} } Inveresk, Musselburgh, Midlothian EH21 8PR, UK
} } Tel. +44 (0) 131 665 6062 / Mobile 0410 585 401
} } ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
} }
}
} -------- REPLY, End of original message --------
}
}


=====================================================================
DR CHRIS JEFFREE
BIOSEM - BIOLOGICAL SCIENCES EM FACILITY
UNIVERSITY OF EDINBURGH
Daniel Rutherford Building
King's Buildings, Mayfield Road
EDINBURGH, EH9 3JH, Scotland, UK
Tel. #44 131 650 5345
FAX. #44 131 650 6563
Mobile 0410 585 401
email c.jeffree-at-ed.ac.uk
SEM / TEM bookings sem-at-ed.ac.uk
=====================================================================



From daemon Mon May 29 08:06:37 2000



From: jim :      jim-at-proscitech.com.au
Date: Mon, 29 May 2000 21:41:45 +1000
Subject: RE: Tissue Drier/ or CPD

Contents Retrieved from Microscopy Listserver Archives
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When using published methodology, some things matter, others do not.
How to know which and what? I don't know, other then a good understanding of
the field.
Anyway, I have been amused over time with researchers insisting on outdated or
cumbersome methods, because "it was published".
In this particular case I would like to note that the tissue dryer is a freeze
dryer designed for microscopy samples. Several other such instruments would do
equally well and I expect that rather more researchers have prepared yeast by
the critical point method.
Freeze drying or critical point drying both work well for numerous samples.
There will be a few specimens that are better prepared by one means or the
other, but I wonder how frequently the claim "this gives better preservation"
should have the disclaimer "in my hands" added.

Point is that you want to dry the yeast for SEM and you may be wasting your
time chasing a particular instrument, when another, perhaps already in the
department would do equally well.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Sunday, May 28, 2000 3:39 AM, Tony Kowal [SMTP:askowal-at-midway.uchicago.edu]
wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear Fellow Microscopists -
} Someone in the lab in which I work wants to do SEM of yeast colonies. I
} was able to locate some references and a general protocol, which seems
} rather straight forward. However, the authors two of the papers refer to
} an "Edwards Pearce Tissue Drier, EPD3". I contacted Edwards to find out
} what this tissue drier is, however they said they could not give me any
} info because it is no longer manufactured.
} I am thinking that this tissue drier is simply a freeze drier, but I am not
} sure. Does anyone out there have any insight into what this thing is or
} what it does? Thanks for your help!!
}
} Tony
}
}
} Tony Kowal
} Research Assistant
} for
} Dr. Susan Lindquist
}
} Howard Hughes Medical Institute
} The University of Chicago
} 5841 S. Maryland
} MC 1028, Room N339
} Chicago, IL 60637
}
} Phone: (773) 702-8795
} Fax: (773)702-7254
} e-mail: askowal-at-midway.uchicago.edu
} Pager: on campus - 188 - 9668 (YNOT)
} off campus - (773)753-1880 - 9668
}
}



From daemon Mon May 29 19:15:13 2000



From: Barbara Foster :      mme-at-map.com
Date: Mon, 29 May 2000 13:12:19 -0400
Subject: Re: charred plant seeds

Contents Retrieved from Microscopy Listserver Archives
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Paul,

Has she looked at them with conventional light microscopy, either stereo or
compound? Following Oxam's Razor, this approach seems to be the simplest
and should be tried first.

Best regards
Barbara Foster,President
Microscopy/Microscopy Education
125 Paridon Street Suite 102
Springfield, MA 01118-2130
PH: 413-746-6931 FX: 413-746-9311 email: MME-at-map.com
Website: www.MME-Microscopy.com/education

America's first national consortium of microscopy specialists offering
customized on-site training in all areas of microscopy, image analysis, and
sample preparation
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^



At 04:19 PM 5/27/00 EDT, Pbgrover-at-aol.com"-at-sparc5.microscopy.com wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Tue May 30 11:10:39 2000



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Tue, 30 May 2000 09:13:06 -0500
Subject: charred plant seeds

Contents Retrieved from Microscopy Listserver Archives
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Paul,

It's been awhile since I was involved in this kind of research (i.e.,
graduate work in palaeoethnobotany, but I do not recall that seeds need to
be "charred", per se, to be preserved over large periods of time. I believe
that seeds and other plant remains can also become carbonized without fire,
as a result of oxidation, etc., in the depositional environment.

To detect charring in the SEM might be a real problem, especially if trying
to differentiate it from carbonization by means other than fire. I suppose
that "charred" seeds might show physical damage more than other seeds, but
even that might not always be true. The charging effects you observed, on
the other hand, might possibly be the effects of mineralization of the seeds
as the original seed components become replaced with non-conductive soil
constituents over time. If so, this might indicate that these seeds are
indeed quite old.

I guess if I was faced with this problem as an archaeologist I would try to
identify other indications of fire in the context in which the seeds were
found, such as wood charcoal, hearths, etc. On the EM side, if I had access
to WDS, I might try comparing amounts of carbon in the old seeds, versus
fresh ones.

Finally, the simplest thing to try might just be to toast some fresh seeds
and compare them to non-toasted ones of the same type. Try a couple
different charring methods, like throwing some in a campfire and collecting
them later, and toasting them on a frying pan. A reference you might look
at is "Palaeoethnobotany", a book by Dr. Deborah Pearsall published by
Academic Press. I think it just came out in a new edition.

Good luck.

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/




-----Original Message-----
} From: "Pbgrover-at-aol.com"-at-sparc5.microscopy.com
[mailto:"Pbgrover-at-aol.com"-at-sparc5.microscopy.com]
Sent: Saturday, May 27, 2000 3:19 PM
To: Microscopy-at-sparc5.microscopy.com


Esteemed colleagues,

A student has asked me if I can find a way to tell whether small plant seeds

have been charred or not. She is examining seeds from an anthropological
dig
on a site probably inhabited both in historic times (170 years ago) and
prehistoric (1000+ years ago). It seems that for seeds to date from the
earlier inhabitation, they would need to be charred, which apparently
preserves them.

I looked at some (uncoated) in the SEM, along with some 'fresh' seeds of
same
species as controls. The seeds of unknown age showed a lot more charging
than the fresh ones. Could this be evidence that they are uncharred (i.e.,
would charring make them more conductive/better secondary electron
producers?) Does anyone know of a method of differentiating between charred

& uncharred seeds?

Thank you thank you thank you :0)

Paul Grover
Chief Microscopist and Bottle Washer
Microvista Laboratory
Lafayette, IN



From daemon Tue May 30 15:41:05 2000



From: Peter Bond :      P.Bond-at-plymouth.ac.uk
Date: Tue, 30 May 2000 11:11:23 -0500
Subject: Film vs Digital

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Hi all

The continuing discussion on whether film is better than digital, and
whether we can directly compare their resolving ability is very interesting
and throwing up some really useful technical stuff. But aren't we missing
the point a bit? Surely the image quality is user defined - if you or your
customer, are satisfied with the end product then the equipment has done
its job. How often does one need to examine a micrograph to assess its
limits? If are getting that close to a picture then you may be taking
things out of context a bit and losing the whole concept.

The future is with digital image capture, let's hope the price of the
equipment comes down to something more attainable!

Pete

Peter Bond
Plymouth Electron Microscopy Unit
University of Plymouth
Drake Circus
Plymouth
Devon
UK PL4 8AA
Tel/Fax: 01752 233092
email: pbond-at-plymouth.ac.uk




From daemon Tue May 30 15:41:05 2000



From: Steve Chapman :      protrain-at-emcourses.com
Date: Mon, 29 May 2000 22:49:54 +0100
Subject: BSE Images

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Hi

A discussion is developing from my comment that a de-saturated W hairpin
source provides a better BSE image. This "problem" first came to light when
a South African client commented that they have far better atomic number
contrast images if they run on "first peak" rather than gun saturation.

I based my explanation on some work by C-R Peters, he relates BSE and SE
signals to probe size. The bigger the probe the bigger the reaction volume
that is the source of the BSE contribution to an image.

In the de-saturated state a normal W hairpin system has a very much bigger
source which reflects in a very much larger probe being placed on the
specimen than would be obtained under a "normal saturation" situation and
therefore provides the operator with more BSE.

Some instruments do give a higher signal at their false peak than at what we
would know as saturation. I have seen this on Cambridge (Leica, Leo)
instruments and no matter how hard you try to "correct" what you feel is a
mis alignment you just do not win. On some occasions I have also seen this
on a Philips but it is much more rare.

I put this down to gun design as you do not see this in a Japanese designed
instrument. In their case a higher false peak indicates a definite mis
alignment!

I base my comments on running courses with a different SEM in a different
laboratory almost every week, not on what happens with one laboratory on one
instrument.

Steve Chapman
Senior Consultant Protrain
For consultancy and professional training in EM world wide
Tel 44+ 1280 814774 Fax 814007
www.emcourses.com



From daemon Tue May 30 15:41:05 2000



From: HILDEGARD CROWLEY :      hcrowley-at-du.edu
Date: Tue, 30 May 2000 11:23:33 -0600 (MDT)
Subject: Epoxy in Path-Research Labs

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Laboratories who have to do pathology work and research also, have
particular problems in that they are faced with a wide variety of
specimens not all of which are suitable for a single epoxy formulation
mixture during embedment.

Many pathology laboratories in the US use the medium hard formulation by
Luft. This is indeed a good, nearly all purpose formulation, however, in
laboratories that have to use glass knives for thick sectioning the NMA
contained in this formulation deterioates the edge of the glass knife very
quickly. If glass knives have to be used for thin sectioning, then this
formulation cannot be used at all. Otherwise with diamond knives it cuts
very well, assuming of course, that the embedding has been done well.
The original formulation by Luft required two mixtures to be made up
seperately. Years ago I combined this into a single mixture which can be
easily frozen (without accelerator!) and kept for months at -80 or -20.

Eponate 12 148 ml
DDSA 100 ml
NMA 76 ml

This makes 324 ml. Accelerate it with 1.5% DMP-30 before use. If you
are interested in this formulation, please print it out now, because this
is the last time I will address it.

Sometimes laboratories are faced with processing floating cells. These
ar e best enrobed in agarose, hopefully after osmication. In order to
avoid enormous cell loss during spinning down repeatedly into embedding
medium, it is fortuitous to draw off all buffer after osmium, and spin the
cells into a mixture of 3% Ultra-low temperature agarose, Type IX (Sigma).
This agarose type gells at 15 deg C, and it has the property of not being
so dense and fibrous that embedding medium cannot penetrate it. After the
gel is cooled (in the refrigerator, or on ice), the blocks are cut of
desired size. It is important to note that one is no longer dealing with
cells, but actual blocks and the protocol for dehydration and embedding
must fit the requirements of the block size. The resulting blocks also
are very well embedded in the above formulation.

The above formulation is good for skin and muscle, but the protocol must
be adjusted to allow adequate penetration. We do 2 hours for dehydration,
use propylene oxide for an intermediate, and start infiltrating with
mixtures of PO and Epoxy, 2:1, 1:1, 1:3, pure, pure, pure, etc. When the
tissue goes into the newly accelerated pure mixtures, I put an ordinary
60W bulb in the vicinity so that the mixture can heat to about 37 deg. At
this point the formulation becomes very liquid and infiltration is
enhanced. After 1.5 hours the lamp is turned off as minor polymerization
begins about 40 deg C, which is undesirable during infiltration.

A problem arises when laboratories have to produce a large number of thick
sections with glass knives, or they have to also use glass knives for thin
sectioning. Then the above formulation cannot be used. A new embedding
medium containing no NMA is needed. Many years ago, Mollenhauer invented
a "Mixed Embedding" system which contained Araldite 502, Epon, DDSA, and
dibutyl pthalate. This is an extremely useful system - a joy to section
with glass knives. The downside is that it is more viscous than media
without Araldite, and it takes longer to infiltrate. Here is the
formulation, again reconfigured by me years ago into a single mixture.

Araldite 502 30ml
Eponate 12 50 ml
DDSA 55 ml
Dibutyl (EMS) 0.75% (mix in with the 3 items above
DMP-30 1.5% (add at time of use)

Freeze mixture without the accelerator. Will keep many months at -80. Can
also keep at -20. For thick sectioning I polymerize just 24 hours. If
blocks are to be thin sectioned, then I put them back into the oven for
another 24 or 48 hours. This formulation has the capability of being soft
and stiff, as you wish. If only thick sections are to be done, use 1%
dibutyl. If blocks are too soft for your liking, just put them into a 60
deg C oven for several days, or use 95 deg C for an hour or two. One time
I forgot some blocks in the 95 deg oven for a week. They still sectioned
well.

Again, anyone interested please print this out now. I will not be
answering these questions again, nor write these formulations again.

This is fun! Keep accurate records of what you do, particularly the
infiltration and dehydration times. Finally you will have every block a
joy to handle. No frustrations!

Bye,
Hildy Crowley
Sr. Electron Microscopy Specialist
University of Denver
Denver, CO

P.S. It is assumed that all processing is done with vials in constant
motion! Once infiltration starts, vials must be on a rotator, not on a
rocker or a shaker, as the monomers of the formulations will seperate and
poor embedding will result.



From daemon Tue May 30 15:41:07 2000



From: William Tivol :      tivol-at-wadsworth.org
Date: Tue, 30 May 2000 15:17:51 -0400
Subject: Re: Film vs Digital

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Peter Bond wrote:

} The continuing discussion on whether film is better than digital, and
} whether we can directly compare their resolving ability is very interesting
} and throwing up some really useful technical stuff. But aren't we missing
} the point a bit? Surely the image quality is user defined - if you or your
} customer, are satisfied with the end product then the equipment has done
} its job. How often does one need to examine a micrograph to assess its
} limits? If are getting that close to a picture then you may be taking
} things out of context a bit and losing the whole concept.
}
} The future is with digital image capture, let's hope the price of the
} equipment comes down to something more attainable!
}

Dear Pete,
If one is doing quantitative image processing, the better
resolution
available from film can be relevant. For example, if one wants to do
corellation averaging, one needs as many objects in the picture as possible,
and also as good resolution as needed--e.g., for 1 nm resolution of the
reconstruction, a pixel size of 0.25 nm times the magnification is necessary,
so the recording medium must have resolution equal to or better than that.
The pixel size of the scanner must also be this small--obviously irrelevant
for digital recording. A 5 micrometer scanning-pixel size at 20kx mag
gives a pixel resolution of 0.25 nm, and for 6.5 by 9 cm film, one will have
about 12,000 by 16,000 (taking the header into account) pixels of useful
information. This will give a broader area than that available at equal
resolution from any CCD chip now out there. I can assure you that I have
often had to determine the information limits in a particular micrograph.
Yours,
Bill Tivol




From daemon Tue May 30 15:41:07 2000



From: Tamara Howard :      howard-at-cshl.org
Date: Tue, 30 May 2000 15:21:25 -0400 (EDT)
Subject: curing Epon/Araldite with UV?

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Greetings!

Has anyone ever cured Epon-Araldite with UV? I was given a
cobbled-together protocol by a non-microscopist post-doc; the protocol
calls for a dry-ice, UV cure of Epon-Araldite. It says it can take from
3-7 days. I'm wondering if this is either a resin mix-up *or* the resin
just set up all by itself (over that time frame I've seen it happen), just
because it was a thin layer and left alone - nothing to do with the UV
exposure. She has no ref. for this technique and isn't sure now where she
got it. Sigh. I've only been able to find heat-cure protocols...anyone
have any leads or thoughts on this UV thing?

(Sorry about the cross-post for those of you on both of these servers)

Thanks!

Tamara (Planning to use the oven.....) Howard
CSHL



From daemon Tue May 30 17:42:26 2000



From: =shAf= :      mshaf-at-darkwing.uoregon.edu
Date: Tue, 30 May 2000 15:28:04 -0700
Subject: RE: BSE Images

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Steve Chapman writes ...

} ...
}
} I based my explanation on some work by C-R Peters, he
} relates BSE and SE signals to probe size.
} The bigger the probe the bigger the reaction volume
} that is the source of the BSE contribution to an image.
}
} In the de-saturated state a normal W hairpin system has a
} very much bigger source which reflects in a very much
} larger probe being placed on the specimen than would be
} obtained under a "normal saturation" situation and
} therefore provides the operator with more BSE.


I have seen the 1st peak provide more beam current, but I fail to see
how a larger probe diameter at similar beam currents can provide
better z contrast. Do you know what the beam current is ... "1st
peak" vs "saturated"??

I might also suggest the 1st peak may provide emission from several
areas of the filament instead of just from the tip. This would
manifest as "ghost" images or double images ... leastwise, I hope this
type of phenomenon couldn't be confused with "better z contrast" :o)

=shAf=



From daemon Tue May 30 21:10:39 2000



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Wed, 31 May 2000 12:17:58 GMT+1200
Subject: Stage drive motors for 840A

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Hi

Does anyone know of anybody who sells motors with suitable mechanical
interfaces to drive X and Y of a JEOL 840A stage?

Replies from suppliers very welcome.

thanks

rtch


Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand



From daemon Tue May 30 21:10:40 2000



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Wed, 31 May 2000 12:51:08 GMT+1200
Subject: PCD for 840

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Hi again

This is my last posting, I promise, looking for a Probe Current
Detector (PCD) for a JEOL 840.
The pneumatically-powered Faraday Cup which shoots into the beam just
below the objective aperture, samples the beam, and retracts.

Does anyone have one which is redundant or otherwise spare?

Alternatively, does anyone know of a third-party manufacturer of this
sort of thing?

cheers

rtch


Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand



From daemon Tue May 30 21:10:40 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Tue, 30 May 2000 18:05:47 -0700
Subject: Re: Film vs Digital

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At 12:17 PM 5/30/00, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

The best CCD imagers are typically 6u in size. Complicating this
is that they may be square or rectangular. Fuji's new hex-shaped
pixels may be a major improvement. We'll see.

gg



From daemon Wed May 31 07:11:30 2000



From: Hans Brinkies :      HBrinkies-at-groupwise.swin.edu.au
Date: Wed, 31 May 2000 12:13:17 +1000
Subject: Our civil engineering department is trying to look at the

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Our civil engineering department is trying to look at the bonding
between bitumen and aggregate with the aid of a SEM. However, we do not
have ESEM facilities (as I suggested). Now I was ask to see if anyone in
the EM fraternity has any experience in using standard SEMs for
examining bitumen containing specimens (I have not!!; and I don't want
to ruin my microscopes).The supplied samples have been cut into slices
of approximately 4mm thickness.

Any ideas out there?
Thanks



Hans Brinkies
Senior Lecturer
Swinburne, University of Technology
School of Engineering and Science
P.O.Box 218 - Hawthorn - Vic -3122 - Australia
Phone: +61 3 9214 8657
Fax: +61 3 9214 8264
Email: Hbrinkies-at-swin.edu.au



From daemon Wed May 31 07:11:31 2000



From: jim :      jim-at-proscitech.com.au
Date: Wed, 31 May 2000 11:13:10 +1000
Subject: RE: Film vs Digital

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Peter Bond is right in saying "The future is with digital image capture" if we
were at some future date to count heads of digital versus film TEM users.
That change will be for reasons of convenience and to save labour. These are
powerful and valid reasons.

Bill Tivol has given one set of applications where digital currently does not
always measure up to film.
Here are another couple of such applications.
1 When great enlargements are required film is superior. This is because of
film's greater resolution, but more importantly, because its much, much easier
to take images at moderate powers and highly enlarge. That way we take
advantage of the TEM's greater depths of focus at low powers and of film's
higher resolution/ image detail.
2 Whenever a TEM image is taken at low brightness (to avoid beam damage or at
very high powers or in dark-field) relatively few electrons form the image and
make that image grainy. Film is very slow and requires then a longer exposure,
thus boosting the quantity of electrons used and improving the image. Digital
is much more sensitive and so the exposure must be shortened. As a result the
best digital camera will record, quiet faithfully the grainy, unsharp image.

Many labs rarely or never use such applications and for them the reasons to
change to digital now may be overwhelming.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Wednesday, May 31, 2000 5:18 AM, William Tivol [SMTP:tivol-at-wadsworth.org]
wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Peter Bond wrote:
}
} } The continuing discussion on whether film is better than digital, and
} } whether we can directly compare their resolving ability is very interesting
} } and throwing up some really useful technical stuff. But aren't we missing
} } the point a bit? Surely the image quality is user defined - if you or your
} } customer, are satisfied with the end product then the equipment has done
} } its job. How often does one need to examine a micrograph to assess its
} } limits? If are getting that close to a picture then you may be taking
} } things out of context a bit and losing the whole concept.
} }
} } The future is with digital image capture, let's hope the price of the
} } equipment comes down to something more attainable!
} }
}
} Dear Pete,
} If one is doing quantitative image processing, the better
} resolution
} available from film can be relevant. For example, if one wants to do
} corellation averaging, one needs as many objects in the picture as possible,
} and also as good resolution as needed--e.g., for 1 nm resolution of the
} reconstruction, a pixel size of 0.25 nm times the magnification is necessary,
} so the recording medium must have resolution equal to or better than that.
} The pixel size of the scanner must also be this small--obviously irrelevant
} for digital recording. A 5 micrometer scanning-pixel size at 20kx mag
} gives a pixel resolution of 0.25 nm, and for 6.5 by 9 cm film, one will have
} about 12,000 by 16,000 (taking the header into account) pixels of useful
} information. This will give a broader area than that available at equal
} resolution from any CCD chip now out there. I can assure you that I have
} often had to determine the information limits in a particular micrograph.
} Yours,
} Bill Tivol
}
}



From daemon Wed May 31 07:11:33 2000



From: gao-at-nirim.go.jp (Gao Yihua)
Date: Wed, 31 May 2000 14:09:13 +0900 (JST)
Subject: Could someone help me to solve the question

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Dear Colleagues:

I have a question about the structure of 2H-SiC (x-ray card
No.29-1130), which belong to No. 186 space group (P63mc). (a=0.3076 nm,
c=0.5048 nm, alfa=beta=120 degrees, gama=90 degrees)

My question is as below:

(A) Based on the symmetry of space group No. 186, the coordinates of
the atoms in a unit cell should be

Si 2a : (0,0,0), and (0,0,0.5)

C 2b : (2/3, 1/3, 0.125) and (1/3,2/3,0.625)


(B) However, acoording to the real stacking sequence, the
coordinates of the Si and C atoms in a unit cell are

Si sites: (0, 0, 0) and (2/3, 1/3, 0.5)
C sites: (2/3, 1/3, 0.125) and (0, 0, 0.625)


It seems that there are some conflict between the two sets of
coordinates, could someone help me to solve such a problem.


Thank you vert much

Yours sincerely
Gao Yihua






From daemon Wed May 31 07:11:34 2000



From: Dennis Ward :      DCWard-at-concentric.net
Date: Wed, 31 May 2000 04:37:07 -0700
Subject: EMSA format

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Could someone direct me to a reference that describes the EMSA formats for
spectra?
Thanks.

Dennis.

_________________________________________________________________________
Dennis C . Ward voice: 202-324-2982
FBI Laboratory fax: 202-324-4018
Microanalysis Laboratory email:
DCWard-at-concentric.net



From daemon Wed May 31 07:11:36 2000



From: David Vowles :      djv23-at-msm.cam.ac.uk
Date: Wed, 31 May 2000 12:37:47 +0100 (BST)
Subject: Re: Stage drive motors for 840A

Contents Retrieved from Microscopy Listserver Archives
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On Wed, 31 May 2000, Ritchie Sims wrote:

} Does anyone know of anybody who sells motors with suitable mechanical
} interfaces to drive X and Y of a JEOL 840A stage?
}
} Replies from suppliers very welcome.

Ritchie,

Deben UK Ltd, 11-15 High St, Stowmarket, Suffolk UK IP14 6QL make very
nice motors and controllers for X, Y and Z control of SEM stages. You can
also contact them at : info-at-deben.co.uk or via their webpage :
http://www.deben.co.uk

Cheers,

David Vowles
Electron Microscopy Unit
Dept of Materials Science and Metallurgy
University of Cambridge
Pembroke St Cambridge
UK CB2 3QZ
Tel: +44 1223 334325
Fax: +44 1223 334567
Email: djv23-at-msm.cam.ac.uk




From daemon Wed May 31 07:11:36 2000



From: Jonathan Barnard :      Jonathan.Barnard-at-angstrom.uu.se
Date: Wed, 31 May 2000 13:41:17 +0200
Subject: Atom locations with P6_3mc

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Dear Gao,
we have used simulation software for CBED for both ZnO and GaN which are
isomorphic with 2H-SiC (same space group:P6_3mc). For our software we use
the following atom locations (I will use Si & C instead):

Si positions: (1/3, 2/3, 0) and (2/3, 1/3, 1/2)

C positions: (1/3, 2/3, u) and (2/3, 1/3, u + 1/2)

The Si-C bond length is parameterized by the quantity u. For the ideal
(perfect stacking) arrangement u=3/8. However I am reliably informed that
for SiC the u parameter is a great mystery at the present. However using
u=3/8 should prove to be a good starting point.

Please note that to place Si at (0,0,0) you will have to remove (1/3,2/3,0)
from each coordinate above which gives the following locations:

Si: (0,0,0) and (1/3, 2/3, 1/2)

C: (0,0,u) and (1/3, 2/3, u + 1/2)

Does this make sense?

Regards,
Jonathan
********************************************************
Dr Jonathan Barnard

Analytical Materials Physics
The Angstrom Laboratory, Uppsala University
P O Box 534, SE-751 21 Uppsala, Sweden
Phone: +46-(0)18-4716838 Fax: +46-(0)18-500131
Phone: Microscope room +46 18 471 6365
http://www.angstrom.uu.se/analytical/home.html
********************************************************



From daemon Wed May 31 07:21:42 2000



From: DAVID I SAXON :      DISAXON-at-prodigy.net
Date: Wed, 31 May 2000 07:19:59 -0500
Subject: roughness of polyethylene

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Dear List,
I'm looking for methods for measuring the roughness of a sheet of
polyethylene. The film consists of grains (80 to 150 microns in diameter)
which are loosely packed. Large area analysis is prefered since the surface
is very irregular locally.

TIA for any suggestions,
David Saxon




From daemon Wed May 31 09:50:24 2000



From: HDMHOS-at-aol.com
Date: Wed, 31 May 2000 09:31:38 EDT
Subject: cryo epoxy

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Hello everybody:
I will like to do cryomicrotomy of PP wires. My question is : can anyone
suggest what kind of epoxy is appropriate for cryomicrotomy?
I used Epoxy Mount and it was chattered during cryo process. The
manufacturer confirm to me that epoxy mount is not appropriate for
cryomicrotomy. Can anynone help me?

Thank You

Briget
Email: HDMHOS-at-AOL.COM



From daemon Wed May 31 09:50:24 2000



From: Leona Cohen-Gould :      lcgould-at-mail.med.cornell.edu
Date: Wed, 31 May 2000 08:42:51 -0400
Subject: Re: Epoxy in Path-Research Labs

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HI Hildy,
You are always a great source of information....thanks.

Lee

Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Confocal Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175




From daemon Wed May 31 09:50:25 2000



From: wft03-at-health.state.ny.us
Date: Wed, 31 May 2000 10:27:45 -0400
Subject: RE: Film vs Digital

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2 Whenever a TEM image is taken at low brightness (to avoid beam damage
or at
very high powers or in dark-field) relatively few electrons form the image
and
make that image grainy. Film is very slow and requires then a longer
exposure,
thus boosting the quantity of electrons used and improving the image.
Digital
is much more sensitive and so the exposure must be shortened. As a result
the
best digital camera will record, quiet faithfully the grainy, unsharp
image.


Dear Jim,

Your points are good; however, exposure times are not, by

themselves, relevant. An exception to this is if the damage depends

on the dose rate; i.e., if there are mechanisms to dissipate the absorbed

beam energy which limit damage at low illumination levels. The

image quality depends on how much information is carried by each elec-

tron (basically, the number of photons produced in the scintillator times

the quantum efficiency of the system for these photons for digital, and the

probability of a darkened film grain for film or image plates) times the

number of electrons. The damage--except for dose-rate-dependence--

is linear with the number of electrons, so it is the efficiency of the pro-

cess of obtaining the information from each electron which determines

the ultimate resolution limit. Also, the use of image averaging can make

one clear image from many grainy ones in the case that one has a large

number of nominally identical specimens.

Yours,

Bill




From daemon Wed May 31 10:19:45 2000



From: Huggins, Bradley J :      HUGGINBJ-at-bp.com
Date: Wed, 31 May 2000 09:49:38 -0500
Subject: RE: charred plant seeds

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Randy,
Don't forget about other analytical techniques, such as XPS. Some well
engineered surface analysis may reveal significant chemical differences
between "charred", new, and aged or mineralized specimens. In addition to
Randy's good advice on the microscopy, I would also utilize the power of
both TEM and EELS in comparing morphology and carbon and oxygen chemistry.
Electron energy loss spectroscopy combined with EDS is one of my favorites
for carbon micro-analyses. Looking at both the low loss and the core loss
structure of EELS spectra, there is tremendous detail and differentiation to
be obtained. If TEM and "PEELS" are available, and you don't mind
sacrificing a bit of the artifact, a series of control specimens and
analysis by "AEM" (TEM, EDS, and PEELS) would be worth a shot.
Sounds like fun, Good Luck,
Brad Huggins
BP Amoco, Analytical
Naperville IL

} ----------
} From: Tindall, Randy D.[SMTP:TindallR-at-missouri.edu]
} Sent: Tuesday, May 30, 2000 9:13 AM
} To: '"Pbgrover-at-aol.com"-at-sparc5.microscopy.com'
} Cc: 'microscopy-at-sparc5.microscopy.com'
} Subject: RE: charred plant seeds
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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} -----------------------------------------------------------------------.
}
}
} Paul,
}
} It's been awhile since I was involved in this kind of research (i.e.,
} graduate work in palaeoethnobotany, but I do not recall that seeds need to
} be "charred", per se, to be preserved over large periods of time. I
} believe
} that seeds and other plant remains can also become carbonized without
} fire,
} as a result of oxidation, etc., in the depositional environment.
}
} To detect charring in the SEM might be a real problem, especially if
} trying
} to differentiate it from carbonization by means other than fire. I
} suppose
} that "charred" seeds might show physical damage more than other seeds, but
} even that might not always be true. The charging effects you observed, on
} the other hand, might possibly be the effects of mineralization of the
} seeds
} as the original seed components become replaced with non-conductive soil
} constituents over time. If so, this might indicate that these seeds are
} indeed quite old.
}
} I guess if I was faced with this problem as an archaeologist I would try
} to
} identify other indications of fire in the context in which the seeds were
} found, such as wood charcoal, hearths, etc. On the EM side, if I had
} access
} to WDS, I might try comparing amounts of carbon in the old seeds, versus
} fresh ones.
}
} Finally, the simplest thing to try might just be to toast some fresh seeds
} and compare them to non-toasted ones of the same type. Try a couple
} different charring methods, like throwing some in a campfire and
} collecting
} them later, and toasting them on a frying pan. A reference you might look
} at is "Palaeoethnobotany", a book by Dr. Deborah Pearsall published by
} Academic Press. I think it just came out in a new edition.
}
} Good luck.
}
} Randy
}
} Randy Tindall
} EM Specialist
} Electron Microscopy Core Facility
} W122 Veterinary Medicine
} University of Missouri
} Columbia, MO 65211
} Tel: (573) 882-8304
} Fax: (573) 884-5414
} Email: tindallr-at-missouri.edu
} Web: http://www.biotech.missouri.edu/emc/
}
}
}
}
} -----Original Message-----
} } From: "Pbgrover-at-aol.com"-at-sparc5.microscopy.com
} [mailto:"Pbgrover-at-aol.com"-at-sparc5.microscopy.com]
} Sent: Saturday, May 27, 2000 3:19 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: charred plant seeds
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Esteemed colleagues,
}
} A student has asked me if I can find a way to tell whether small plant
} seeds
}
} have been charred or not. She is examining seeds from an anthropological
} dig
} on a site probably inhabited both in historic times (170 years ago) and
} prehistoric (1000+ years ago). It seems that for seeds to date from the
} earlier inhabitation, they would need to be charred, which apparently
} preserves them.
}
} I looked at some (uncoated) in the SEM, along with some 'fresh' seeds of
} same
} species as controls. The seeds of unknown age showed a lot more charging
}
} than the fresh ones. Could this be evidence that they are uncharred
} (i.e.,
} would charring make them more conductive/better secondary electron
} producers?) Does anyone know of a method of differentiating between
} charred
}
} & uncharred seeds?
}
} Thank you thank you thank you :0)
}
} Paul Grover
} Chief Microscopist and Bottle Washer
} Microvista Laboratory
} Lafayette, IN
}



From daemon Wed May 31 18:47:29 2000



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Wed, 31 May 2000 09:31:24 -0500
Subject: RE: charred plant seeds

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Could it be that moisture escaping from the fresh seeds somehow helps to
dissipate the charge so that new seeds do not charge like the old ones?

I would also be interested in the x-ray spectra of the two seeds. Perhaps
there is partial mineralization as Randy Tindall suggested. A light element
detector should also reveal a difference in O/C ratio due to differences in
moisture content, or perhaps due to charring.

Warren S.

At 09:13 AM 5/30/2000 -0500, you wrote:
} Esteemed colleagues,
}
} A student has asked me if I can find a way to tell whether small plant seeds
}
} have been charred or not. She is examining seeds from an anthropological
} dig
} on a site probably inhabited both in historic times (170 years ago) and
} prehistoric (1000+ years ago). It seems that for seeds to date from the
} earlier inhabitation, they would need to be charred, which apparently
} preserves them.
}
} I looked at some (uncoated) in the SEM, along with some 'fresh' seeds of
} same
} species as controls. The seeds of unknown age showed a lot more charging
} than the fresh ones. Could this be evidence that they are uncharred (i.e.,
} would charring make them more conductive/better secondary electron
} producers?) Does anyone know of a method of differentiating between charred
}
} & uncharred seeds?
}
} Thank you thank you thank you :0)
}
} Paul Grover
} Chief Microscopist and Bottle Washer
} Microvista Laboratory
} Lafayette, IN



From daemon Wed May 31 18:47:29 2000



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Wed, 31 May 2000 09:17:51 -0600
Subject: Re: Film vs Digital

Contents Retrieved from Microscopy Listserver Archives
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Of course there are situations where one or the other Technique may be
better because of the limitations of film or digital imaging. And
especially for a task like the one Bill talks about below, where field
of view AND resolution are important, images taken on film may be
superior to images from a digital camera. On the other hand, digital
imaging may have something to offer in those cases as well:

Instead of taking one image and do the corellation averaging, why not
have the computer do it? I could probably set up a system that acquires
the image at a high enough resolution, extract all the necessary data,
then move the stage to an adjacent area and continue the measurements
there. This technique would even have an advantage over film: I can set
the lower limits for the accuracy before taking the images and the
system continues to measure until these limits are satisfied. This could
be done without user intervention. So, instead of taking one image,
scanning it in with a scanner, and then being "limited" by the field of
view to a certain measurement accuracy, one could start the measurement
with a predetermined accuracy, go drink a coffee, work on that paper, do
the travel expense report, then go back to the microscope and collect
the spreadsheet with the measurement data.
Of course this requires the setup of a fairly complex system, but those
are technical problems, not fundamental ones.

Just a thought ....


Michael

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: William Tivol [mailto:tivol-at-wadsworth.org]
Sent: Tuesday, May 30, 2000 1:18 PM
To: microscopy-at-sparc5.microscopy.com


Peter Bond wrote:

} The continuing discussion on whether film is better than digital, and
} whether we can directly compare their resolving ability is very
interesting
} and throwing up some really useful technical stuff. But aren't we
missing
} the point a bit? Surely the image quality is user defined - if you or
your
} customer, are satisfied with the end product then the equipment has
done
} its job. How often does one need to examine a micrograph to assess its
} limits? If are getting that close to a picture then you may be taking
} things out of context a bit and losing the whole concept.
}
} The future is with digital image capture, let's hope the price of the
} equipment comes down to something more attainable!
}

Dear Pete,
If one is doing quantitative image processing, the better
resolution
available from film can be relevant. For example, if one wants to do
corellation averaging, one needs as many objects in the picture as
possible,
and also as good resolution as needed--e.g., for 1 nm resolution of the
reconstruction, a pixel size of 0.25 nm times the magnification is
necessary,
so the recording medium must have resolution equal to or better than
that.
The pixel size of the scanner must also be this small--obviously
irrelevant
for digital recording. A 5 micrometer scanning-pixel size at 20kx mag
gives a pixel resolution of 0.25 nm, and for 6.5 by 9 cm film, one will
have
about 12,000 by 16,000 (taking the header into account) pixels of useful
information. This will give a broader area than that available at equal
resolution from any CCD chip now out there. I can assure you that I
have
often had to determine the information limits in a particular
micrograph.
Yours,
Bill Tivol




From daemon Wed May 31 18:47:30 2000



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Wed, 31 May 2000 09:42:40 -0600
Subject: RE: Film vs Digital

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Hello Jim,

just a couple of remarks to your email.

your remark 1) What you can do of course with digital imaging (provided
you have the necessary hardware), is to automatically collect larger
areas by taking several images that overlap, reducing the advantage that
film has in this area and perhaps offer other possibilities as I just
explained in another posting as a response to Bill Tivol's posting.

your remark 2) I am not sure you are not comparing apples and oranges.
What you are saying is, that because of the "slowness" of film you need
longer exposures, thereby averaging out the statistical noise of the
electron, which is not the case for CCD cameras at short exposures,
hence they appear more noisy. In essence what you are doing is to
compare a short exposure image to a long exposure image. What you can do
with a CCD camera is the following: You can get (perhaps) real-time dark
field images and position your sample and/or decide if you want to
actually take the image. Then you take a SERIES of images, let's say 10,
each at an exposure time of 1/10 of the film exposure. This can be done
automatically, of course. Finally, you add or average all of these
images using a pattern recognition to align them first. The result: A
dark field image that should be as noisy as the film image, with much
less problems due to drift during long exposures, a higher dynamic range
and visible immediately on the viewing screen.

Michael

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: jim [mailto:jim-at-proscitech.com.au]
Sent: Tuesday, May 30, 2000 7:13 PM
To: 'William Tivol'
Cc: microscopy-at-sparc5.microscopy.com


Peter Bond is right in saying "The future is with digital image capture"
if we
were at some future date to count heads of digital versus film TEM
users.
That change will be for reasons of convenience and to save labour. These
are
powerful and valid reasons.

Bill Tivol has given one set of applications where digital currently
does not
always measure up to film.
Here are another couple of such applications.
1 When great enlargements are required film is superior. This is
because of
film's greater resolution, but more importantly, because its much, much
easier
to take images at moderate powers and highly enlarge. That way we take
advantage of the TEM's greater depths of focus at low powers and of
film's
higher resolution/ image detail.
2 Whenever a TEM image is taken at low brightness (to avoid beam
damage or at
very high powers or in dark-field) relatively few electrons form the
image and
make that image grainy. Film is very slow and requires then a longer
exposure,
thus boosting the quantity of electrons used and improving the image.
Digital
is much more sensitive and so the exposure must be shortened. As a
result the
best digital camera will record, quiet faithfully the grainy, unsharp
image.

Many labs rarely or never use such applications and for them the reasons
to
change to digital now may be overwhelming.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Wednesday, May 31, 2000 5:18 AM, William Tivol
[SMTP:tivol-at-wadsworth.org]
wrote:
}
------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
} To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
} On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
}
-----------------------------------------------------------------------.
}
}
} Peter Bond wrote:
}
} } The continuing discussion on whether film is better than digital,
and
} } whether we can directly compare their resolving ability is very
interesting
} } and throwing up some really useful technical stuff. But aren't we
missing
} } the point a bit? Surely the image quality is user defined - if you
or your
} } customer, are satisfied with the end product then the equipment has
done
} } its job. How often does one need to examine a micrograph to assess
its
} } limits? If are getting that close to a picture then you may be
taking
} } things out of context a bit and losing the whole concept.
} }
} } The future is with digital image capture, let's hope the price of
the
} } equipment comes down to something more attainable!
} }
}
} Dear Pete,
} If one is doing quantitative image processing, the better
} resolution
} available from film can be relevant. For example, if one wants to do
} corellation averaging, one needs as many objects in the picture as
possible,
} and also as good resolution as needed--e.g., for 1 nm resolution of
the
} reconstruction, a pixel size of 0.25 nm times the magnification is
necessary,
} so the recording medium must have resolution equal to or better than
that.
} The pixel size of the scanner must also be this small--obviously
irrelevant
} for digital recording. A 5 micrometer scanning-pixel size at 20kx mag
} gives a pixel resolution of 0.25 nm, and for 6.5 by 9 cm film, one
will have
} about 12,000 by 16,000 (taking the header into account) pixels of
useful
} information. This will give a broader area than that available at
equal
} resolution from any CCD chip now out there. I can assure you that I
have
} often had to determine the information limits in a particular
micrograph.
} Yours,
} Bill Tivol
}
}



From daemon Wed May 31 18:47:30 2000



From: Michal Jarnik :      M_Jarnik-at-fccc.edu
Date: Wed, 31 May 2000 11:57:49 -0500
Subject: Job Opening

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Job Opening


Electron Microscopy Facility at Fox Chase Cancer Center is seeking a
motivated individual for a Technician/Research Assistant position.

Required qualifications: B.S. or M.S. in biology, 2+ years of experience
in biological electron microscopy.

Responsibilities include sample preparation and electron microscopy
(TEM/SEM), dark room work, computer image processing, report
preparation. The great variability of work due to collaborations with
large number of laboratories provides exceptional opportunity for
professional growth.

We offer a competitive salary commensurate with experience, an excellent
benefit package (health/dental insurance, pension plan, paid vacation)
and a very friendly working environment.

For confidential consideration, please send a CV including a statement
of experience to:

Dr. Michael Jarnik
Fox Chase Cancer Center
EM Facility
7701 Burholme Avenue
Philadelphia, PA 19111
e-mail: m_jarnik-at-fccc.edu



From daemon Wed May 31 18:47:31 2000



From: Lucille A. Giannuzzi :      lag-at-mail.ucf.edu
Date: Wed, 31 May 2000 12:58:30 -0400
Subject: Graduate Students needed at UCF

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MS level and PhD level graduate students are needed for research/education
in the Materials Science and Engineering Discipline at the University of
Central Florida.

Students will also work closely with Cirent Semiconductor (Lucent
Technologies, Orlando, FL) staff scientists.

For more information please contact:

Dr. Lucille A. Giannuzzi
Associate Professor, Mechanical, Materials, and Aerospace Engineering
Director, UCF/Cirent Materials Characterization Facility
University of Central Florida
12443 Research Parkway, Suite 305
Orlando, FL 32826
email lag-at-mail.ucf.edu
phone (407) 275-4354,5,6
fax (407) 275-4321

*******************************************************************
Lucille A. Giannuzzi, Ph.D.

Associate Professor, Mechanical, Materials, and Aerospace Engineering
Director, UCF/Cirent Materials Characterization Facility
University of Central Florida
12443 Research Parkway, Suite 305
Orlando, FL 32826 email lag-at-mail.ucf.edu phone (407) 275-4354,5,6
fax (407) 275-4321
--------------------------------------------------------------------
"Good judgement comes from experience.

Experience comes from making bad judgement."

Mark Twain
********************************************************************




From daemon Wed May 31 18:47:33 2000



From: William Tivol :      tivol-at-wadsworth.org
Date: Wed, 31 May 2000 13:58:52 -0400
Subject: Re: Film vs Digital

Contents Retrieved from Microscopy Listserver Archives
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Mike Bode wrote:

} Instead of taking one image and do the corellation averaging, why not
} have the computer do it? I could probably set up a system that acquires
} the image at a high enough resolution, extract all the necessary data,
} then move the stage to an adjacent area and continue the measurements
} there. This technique would even have an advantage over film: I can set
} the lower limits for the accuracy before taking the images and the
} system continues to measure until these limits are satisfied. This could
} be done without user intervention. So, instead of taking one image,
} scanning it in with a scanner, and then being "limited" by the field of
} view to a certain measurement accuracy, one could start the measurement
} with a predetermined accuracy, go drink a coffee, work on that paper, do
} the travel expense report, then go back to the microscope and collect
} the spreadsheet with the measurement data.
} Of course this requires the setup of a fairly complex system, but those
} are technical problems, not fundamental ones.
}

Dear Mike,
That is an excellent suggestion. Of course, the beam should be

restricted to the area of the detector to avoid damaging the specimen
except where inevitable. You are correct that setting the accuracy
limits prior to taking the image avoids mixing the required data with
those from excess exposure, when the specimen has undergone some
radiation damage--this could perhaps be done with film, but it is
simpler with electronic data collection. There one can even make
sure that those pixels which are most important are the ones optimized,
and, with a small enough beam, like that used in spot-scan imaging,
one could expose different parts of the image for different times, so
that as much as possible of the image is optimally collected. This also
avoids damaging the area of the specimen not yet under investigation.

}
} Just a thought ....
}

And a good one.
Yours,
Bill Tivol



From daemon Wed May 31 18:47:33 2000



From: Jim Ferreira :      ferreira1-at-llnl.gov
Date: Wed, 31 May 2000 11:33:47 -0700
Subject: SEM Stereo Image Analysis Software

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Hello folks


Anyone aware of software capable of generating depth profiles from digital SEM stereo pair images? I am aware of the Oxford ISIS system/program, but wondered if other companies offer similar software that would run 'stand-alone' in a PC and work with any digital files.


Any help or suggestions along these lines would be most appreciated.


Thanks,

Jim




____________________________________________


{bold} {bigger} Scanning Electron Microscopy

{/bigger} {/bold} Jim Ferreira

Material Science & Technology Division

Chemistry & Material Science Directorate

Lawrence Livermore National Laboratory

PO Box 808 L-356, Livermore CA 94550

Ph: 925/424-4470 E-mail: ferreira1-at-llnl.gov



{/x-rich}



From daemon Wed May 31 18:47:34 2000



From: HILDEGARD CROWLEY :      hcrowley-at-du.edu
Date: Wed, 31 May 2000 12:35:01 -0600 (MDT)
Subject: Re: curing Epon/Araldite with UV?

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On Tue, 30 May 2000, Tamara Howard wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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} -----------------------------------------------------------------------.
}
}
} Greetings!
}
} Has anyone ever cured Epon-Araldite with UV? I was given a
} cobbled-together protocol by a non-microscopist post-doc; the protocol
} calls for a dry-ice, UV cure of Epon-Araldite. It says it can take from
} 3-7 days. I'm wondering if this is either a resin mix-up *or* the resin
} just set up all by itself (over that time frame I've seen it happen), just
} because it was a thin layer and left alone - nothing to do with the UV
} exposure. She has no ref. for this technique and isn't sure now where she
} got it. Sigh. I've only been able to find heat-cure protocols...anyone
} have any leads or thoughts on this UV thing?
}
} (Sorry about the cross-post for those of you on both of these servers)
}
} Thanks!
}
} Tamara (Planning to use the oven.....) Howard
} CSHL
}
}
}
Hi,

Try it, but not on anything valuable! Resins set up by themselves at rt.
I don't remember ever reading anything about UV in the Handbook of Epoxy
Resins. Why do you want to do it? That is the question. What would be
the advantage of that major fiddle?

Hildy Crowley



From daemon Wed May 31 18:47:34 2000



From: Rajesh Patel :      rpatel-at-UMDNJ.EDU
Date: Wed, 31 May 2000 14:41:42 -0400
Subject: service

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We need service on our Balzer 500 freeze fractrure unit,
and was wondering who services them. Anyone we can contact?

Rajesh Patel
Robert Wood Johnson Medical School
Dept. of Pathology
Electron Microscopy Lab
675 Hoes Lane
Piscataway, NJ 08854

(732)235-4648
rpatel-at-umdnj.edu



From daemon Wed May 31 18:47:35 2000



From: Barbara Foster :      mme-at-map.com
Date: Wed, 31 May 2000 15:33:16 -0400
Subject: Re: Our civil engineering department is trying to look at

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Hans,

When I worked for Zeiss, we did a lot of coal analysis using
microspectrophotometry. I've also done some polarized light work on a
sample or two for customers. Is the bonding you are looking for on the
level where light microscopy might work? Just for your information, I also
recently took a look at the "tie" layers between polymer films in ketsup
bottles, perhaps a distant but related application. Polarized light and
DIC did a great job in that instance.

.. just a thought, but hopefully helpful.

Best regards,
Barbara Foster,President
Microscopy/Microscopy Education
125 Paridon Street Suite 102
Springfield, MA 01118-2130
PH: 413-746-6931 FX: 413-746-9311 email: MME-at-map.com
Website: www.MME-Microscopy.com/education

America's first national consortium of microscopy specialists offering
customized on-site training in all areas of microscopy, image analysis, and
sample preparation
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^




At 12:13 PM 5/31/00 +1000, Hans Brinkies wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Wed May 31 18:47:35 2000



From: DrJohnRuss-at-aol.com
Date: Wed, 31 May 2000 15:35:07 EDT
Subject: Re: SEM Stereo Image Analysis Software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Have you considered structured lighting? A flat beam of light is illuminates
the subject from a 45 degree angle. A camera is views it at 90 degrees.
The height and size of the roughness can be reconstructed from the shadows
the light makes.

Gordon

Gordon Couger gcouger-at-couger.com

Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00
----- Original Message -----
} From: "DAVID I SAXON" {DISAXON-at-prodigy.net}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Wednesday, May 31, 2000 7:19 AM



In a message dated 5/31/00 2:52:24 PM, ferreira1-at-llnl.gov writes:

} Anyone aware of software capable of generating depth profiles from digital
} SEM stereo pair images? I am aware of the Oxford ISIS system/program,
} but wondered if other companies offer similar software that would run
'stand-alone'
} in a PC and work with any digital files.

One of the (many) functions in Fovea Pro (http://members.aol.com/FoveaPro), a
set of Photoshop-compatible plug-ins intended for the analysis of images
including those from microscopes such as SEMs, is a routine that fuses stereo
pair images to obtain the elevation of points on the surface. This can then
be used to measure elevation profiles, or to reconstruct 3D surface images.
Examples of both are included in the tutorial and are illustrated on the web
site. It isn't stand-alone (you need Photoshop or a compatible program), but
it will do what you ask.




From daemon Wed May 31 18:47:42 2000



From: hpadams :      hpadams-at-bcm.tmc.edu
Date: Wed, 31 May 2000 17:51:23 -0500
Subject: tissue drier

Contents Retrieved from Microscopy Listserver Archives
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The tissue drier you referred to had a "cold finger" which acted as a vapor
trap to remove the water vapor produced from the sublimation of the specimen
ice. This is a very important feature for achieving "distortion-free drying"
which common type of freeze driers lack I believe.
Check out Pearse's book Histochemistry Theoretical and Applied, vol.1. It
contains an excellent chapter on this technique and others. However, I would
try CPD, also.

Hank Adams
Manager
Integrated Microscopy Core
Molecular and Cellular Biology
Baylor College of Medicine
Houston, Tx 77030



From daemon Thu Jun 01 07:11:17 2000



From: P.Wang :      P.Wang-at-sheffield.ac.uk
Date: Wed, 31 May 2000 18:48:09 -0500
Subject: Turbo pump

Contents Retrieved from Microscopy Listserver Archives
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Hello, there,
Does anyone out here know that can we put alkylamine to the turbo pump
when carbon supporting film is being glow discharge?
Thanks.

Peiyi

Krebs Institute for Biomolecular Research
University of Sheffield
Firth Court
Western Bank
Sheffield
Yorkshire S10 2UH
United Kingdom
Tel: +44 (0)114 222 2000
Direct: +44 (0)114 222 2739
FAX: +44 (0)114 272 8697
E-mail: p.wang-at-sheffield.ac.uk




From daemon Thu Jun 01 07:11:18 2000



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Wed, 31 May 2000 20:17:17 -0500
Subject: PP (coated) wires

Contents Retrieved from Microscopy Listserver Archives
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-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Briget wrote:
===========================================================
I will like to do cryomicrotomy of PP wires. My question is : can anyone
suggest what kind of epoxy is appropriate for cryomicrotomy? I used Epoxy
Mount and it was chattered during cryo process. The manufacturer confirm
to me that epoxy mount is not appropriate for cryomicrotomy. Can anynone
help me?
===========================================================
I am assuming you mean polypropylene coated wires and not polypropylene
monofilament. We have generally found that for coated wires samples, we
like to Pt coat it first (by sputtering), then embed in SPI-Pon™ 812 resin.
I would expect that any of the other "Epon® 812 substitutes", available
from the other major EM supplies firms would work just as well.

You will definitely want to use a diamond knife on this and you can vary the
hardness of the resin in way that gives you the best sections. Use a knife
angle that is not larger than 45°, the lower the temperature (usually) the
better.

Disclaimer: SPI Supplies offers for sale the resin and diamond knives
mentioned and performs this kind of cryoultramicrotomy for clients.

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.spi.cc
########################
===========================================



From daemon Thu Jun 01 07:11:18 2000



From: Shane Collins :      kshanec-at-gte.net
Date: Wed, 31 May 2000 17:15:18 -0700
Subject: RE: new developments in imaging systems?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hamamatsu recently introduced a new camera called the electron bombardment
ccd where accelerated electrons directly bombard a back thinned, peltier
cooled ccd. The electrons are emitted from a photocathode for applications
particularly in low light microscopy. With a full well capacity of 300,000
electrons, I wonder if this approach could be used in direct exposure of the
ccd to the electron beam.
Shane

K. Shane Collins
Scientific Instrument Company
805.444.4953 cell
310.568.9188 office
310.568.9189 fax


-----Original Message-----
} From: Paul Voyles [mailto:voyles-at-research.nj.nec.com]
Sent: Friday, May 26, 2000 9:11 AM
To: Microscopy-at-sparc5.microscopy.com


} You could probably expose your CCD chip directly to the electron bean --
} and buy a new chip every few hundred exposures or so! Electrons have

There is an even worse problem with exposing the CCD directly the
electron beam. The p-n junctions in the CCD chip have a certain
"well capacity" - a number of electron/hole pairs they can hold
before they saturate. Fast (keV) electrons are much more efficient
at producing electron/hole pairs than photons - so much so that a
pixel on the CCD would saturate after about 15 fast electrons hit it.
Just the square root N shot noise at that level is about 25% - larger
than typical TEM micrograph contrast of 10-20%. That's why a
phosphor or scintillator is necessary to transform the fast electrons
into photons.




Happy reading,
Paul




From daemon Thu Jun 01 07:11:20 2000



From: jim :      jim-at-proscitech.com.au
Date: Thu, 1 Jun 2000 13:36:52 +1000
Subject: RE: Film vs Digital

Contents Retrieved from Microscopy Listserver Archives
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Yes, it could be done "in theory". Somebody would need to figure out the
software and perhaps modify the hardware. Then we would find that the total
exposure of the specimen to the electron beam maybe a muliple of the film's
exposure. Afterall, an 8 sec film exposure would not amount in digital to
10x0.8, but we would require considerable time in between exposures. Since the
problems in the discussed circumstances are specimen movement and beam damage,
it seems that taking multiple exposures is a poor option.

Digital cameras are for some situation too sensitive to electron exposure.
Cutting back on electrons is no option since its the electrons that form the
image in the first instance.
Much easier in light microscopy . . . insert a neutral density filter.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com


} Hello Jim,
}
} just a couple of remarks to your email.
}
} your remark 1) What you can do of course with digital imaging (provided
} you have the necessary hardware), is to automatically collect larger
} areas by taking several images that overlap, reducing the advantage that
} film has in this area and perhaps offer other possibilities as I just
} explained in another posting as a response to Bill Tivol's posting.
}
} your remark 2) I am not sure you are not comparing apples and oranges.
} What you are saying is, that because of the "slowness" of film you need
} longer exposures, thereby averaging out the statistical noise of the
} electron, which is not the case for CCD cameras at short exposures,
} hence they appear more noisy. In essence what you are doing is to
} compare a short exposure image to a long exposure image. What you can do
} with a CCD camera is the following: You can get (perhaps) real-time dark
} field images and position your sample and/or decide if you want to
} actually take the image. Then you take a SERIES of images, let's say 10,
} each at an exposure time of 1/10 of the film exposure. This can be done
} automatically, of course. Finally, you add or average all of these
} images using a pattern recognition to align them first. The result: A
} dark field image that should be as noisy as the film image, with much
} less problems due to drift during long exposures, a higher dynamic range
} and visible immediately on the viewing screen.
}
} Michael
}
} Michael Bode, Ph.D.
} Soft Imaging System Corp.
} 1675 Carr St., #105N
} Lakewood, CO 80215
} ===================================
} phone: (888) FIND SIS
} (303) 234-9270
} fax: (303) 234-9271
} email: mailto:info-at-soft-imaging.com
} web: http://www.soft-imaging.com
} ===================================
}
}
}
} -----Original Message-----
} } From: jim [mailto:jim-at-proscitech.com.au]
} Sent: Tuesday, May 30, 2000 7:13 PM
} To: 'William Tivol'
} Cc: microscopy-at-sparc5.microscopy.com
} Subject: RE: Film vs Digital
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Peter Bond is right in saying "The future is with digital image capture"
} if we
} were at some future date to count heads of digital versus film TEM
} users.
} That change will be for reasons of convenience and to save labour. These
} are
} powerful and valid reasons.
}
} Bill Tivol has given one set of applications where digital currently
} does not
} always measure up to film.
} Here are another couple of such applications.
} 1 When great enlargements are required film is superior. This is
} because of
} film's greater resolution, but more importantly, because its much, much
} easier
} to take images at moderate powers and highly enlarge. That way we take
} advantage of the TEM's greater depths of focus at low powers and of
} film's
} higher resolution/ image detail.
} 2 Whenever a TEM image is taken at low brightness (to avoid beam
} damage or at
} very high powers or in dark-field) relatively few electrons form the
} image and
} make that image grainy. Film is very slow and requires then a longer
} exposure,
} thus boosting the quantity of electrons used and improving the image.
} Digital
} is much more sensitive and so the exposure must be shortened. As a
} result the
} best digital camera will record, quiet faithfully the grainy, unsharp
} image.
}
} Many labs rarely or never use such applications and for them the reasons
} to
} change to digital now may be overwhelming.
} Cheers
} Jim Darley
} ProSciTech Microscopy PLUS
} PO Box 111, Thuringowa QLD 4817 Australia
} Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
} Great microscopy catalogue, 500 Links, MSDS, User Notes
} www.proscitech.com
}
} On Wednesday, May 31, 2000 5:18 AM, William Tivol
} [SMTP:tivol-at-wadsworth.org]
} wrote:
} }
} ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} } To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} } On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} }
} -----------------------------------------------------------------------.
} }
} }
} } Peter Bond wrote:
} }
} } } The continuing discussion on whether film is better than digital,
} and
} } } whether we can directly compare their resolving ability is very
} interesting
} } } and throwing up some really useful technical stuff. But aren't we
} missing
} } } the point a bit? Surely the image quality is user defined - if you
} or your
} } } customer, are satisfied with the end product then the equipment has
} done
} } } its job. How often does one need to examine a micrograph to assess
} its
} } } limits? If are getting that close to a picture then you may be
} taking
} } } things out of context a bit and losing the whole concept.
} } }
} } } The future is with digital image capture, let's hope the price of
} the
} } } equipment comes down to something more attainable!
} } }
} }
} } Dear Pete,
} } If one is doing quantitative image processing, the better
} } resolution
} } available from film can be relevant. For example, if one wants to do
} } corellation averaging, one needs as many objects in the picture as
} possible,
} } and also as good resolution as needed--e.g., for 1 nm resolution of
} the
} } reconstruction, a pixel size of 0.25 nm times the magnification is
} necessary,
} } so the recording medium must have resolution equal to or better than
} that.
} } The pixel size of the scanner must also be this small--obviously
} irrelevant
} } for digital recording. A 5 micrometer scanning-pixel size at 20kx mag
} } gives a pixel resolution of 0.25 nm, and for 6.5 by 9 cm film, one
} will have
} } about 12,000 by 16,000 (taking the header into account) pixels of
} useful
} } information. This will give a broader area than that available at
} equal
} } resolution from any CCD chip now out there. I can assure you that I
} have
} } often had to determine the information limits in a particular
} micrograph.
} } Yours,
} } Bill Tivol
} }
} }
}



From daemon Thu Jun 01 07:11:22 2000



From: Chris Jeffree :      cjeffree-at-srv0.bio.ed.ac.uk
Date: Thu, 1 Jun 2000 09:52:22 +0000
Subject: SEM Stereo Image Analysis Software

Contents Retrieved from Microscopy Listserver Archives
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The electron energies used in this "intensifier" will be in the order of one
or several kV. In TEM however the energies are much higher thus one incident
electron will generate for example hundred electron-hole pairs in the CCD
thus saturating it very fast. Another factors are damage to the CCD chip and
X-Rays.

I was thinking about another approach. A chip consisting of matrix of
thermo-sensitive elements. Above each element there will be a metal block
with height equal or larger than the stop path for the electron energy used.
The whole thing will be cooled in a similar way as the CCDs. When this
assembly is exposed to the beam each block will increase its temperature
depending on the number of electrons stopped. After the exposure the matrix
is scanned and the temperature increase at each element is measured
(ofcourse before each exposure a reference image has to be taken).

The benefits:
- Very high efficiency. Almost every incident electron will contribute to
the image.
- Huge dynamic range.
- Linearity (after the temperature-signal characteristic of each element has
been calibrated)
- Narrow point spread function (maybe).

Problems:
- Difficult to manufacture (the metal blocks should be insulated from each
other)
- Maybe low sensitivity. I haven't calculated how much the temperature
increase will be (for example 5x5x30um Cu block hit by one 300 kV electron)
but I suspect it will be very small. Also It will depend on the sensitivity
of the thermo-measuring elements.
- Saturation. After each exposure one has to wait some time for the thing to
cool down again.

There are maybe other difficulties which do not come in mind now.

Hmmm now I start thinking about X-Rays. Actually the main part of the
incident energy will go into X-Rays thus making this device useless.

Rado

---------------------------------------------------------------------
Radostin Danev
Laboratory of Ultrastructure Research
National Institute for Physiological Sciences
Myodaiji-cho, Okazaki 444-8585, JAPAN
e-mail: rado-at-nips.ac.jp
---------------------------------------------------------------------
----- Original Message -----
} From: Shane Collins {kshanec-at-gte.net}
To: Paul Voyles {voyles-at-research.nj.nec.com} ;
{Microscopy-at-sparc5.microscopy.com}
Sent: Thursday, June 01, 2000 9:15 AM


AnalySIS has a module which does this
see Soft Imaging's web site at
http://www.soft-imaging.de
Chris

To: Microscopy-at-sparc5.microscopy.com
} From: Jim Ferreira {ferreira1-at-llnl.gov}


=====================================================================
DR CHRIS JEFFREE
BIOSEM - BIOLOGICAL SCIENCES EM FACILITY
UNIVERSITY OF EDINBURGH
Daniel Rutherford Building
King's Buildings, Mayfield Road
EDINBURGH, EH9 3JH, Scotland, UK
Tel. #44 131 650 5345
FAX. #44 131 650 6563
Mobile 0410 585 401
email c.jeffree-at-ed.ac.uk
SEM / TEM bookings sem-at-ed.ac.uk
=====================================================================



From daemon Thu Jun 01 07:21:29 2000



From: Colin MacRae :      cmac-at-minerals.csiro.au
Date: Thu, 1 Jun 2000 07:11:59 -0500
Subject: Re: SEM Stereo Image Analysis Software

Contents Retrieved from Microscopy Listserver Archives
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In a message dated 5/31/00 2:52:24 PM, ferreira1-at-llnl.gov writes:

Anyone aware of software capable of generating depth profiles from digital
SEM stereo pair images? I am aware of the Oxford ISIS system/program, but
wondered if other companies offer similar software that would run
'stand-alone' in a PC and work with any digital files.

Jim
I have been using uMex software for the last 6 months which does
exactly
what you want. Given a stereo pair it will produce a 3D surface
reconstruction. It has a function that enables images to the two images to
be manually aligned prior to the calculation. This function when used
correctly seems to result in faster calculations.

Another other nice feature is that images can be output in VRML file
format. So you can view the images with shareware 3D packages. We use a
SGI for viewing as its rendering speed is significantly faster than a PC.

I suggest you check out the following web site.
http://www.alicona.com/en/products.htm

Regards
Colin MacRae

************************************************************************
Manager of Electron Microscopy Group (Clayton)

CSIRO
Minerals {mailto:colin.macrae-at-minerals.csiro.au}

PO Box 312, Clayton South, Ph. 61 3 9545 8800
Vic, 3169 Fax 61 3 9562 8919
AUSTRALIA

EM units WWW site http://www.minerals.csiro.au/em-unit/
*************************************************************************




From daemon Thu Jun 01 08:41:17 2000



From: Jeremy Sanderson :      jb_sanderson-at-yahoo.com
Date: Thu, 1 Jun 2000 06:31:32 -0700 (PDT)
Subject: Resolving power: film vs digital

Contents Retrieved from Microscopy Listserver Archives
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Dear All,

I've been following the thread on film vs digital.
We all know that the resolving power of digital CCD
faceplates is approaching that of conventional film
emulsion, but isn't equivalent yet.
Can someone remind me of the number of mega-pixels
that a CCD will need to equate to ISO 100 print film,
ISO 64 or ISO100 slide film and the highest resolving
B&W film of all, Technical Pan at ISO 25 and ISO 100?
If anyone out there can lead me through the logic and
the maths, I'm sure others will also find it helpful.
I'll repost a summary of the replies that I get.

Regards, Jeremy Sanderson

__________________________________________________
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Send instant messages & get email alerts with Yahoo! Messenger.
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From daemon Thu Jun 01 16:08:51 2000



From: Paul Voyles :      voyles-at-research.nj.nec.com
Date: Thu, 1 Jun 2000 10:34:26 -0500
Subject: RE: new developments in imaging systems?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} Hamamatsu recently introduced a new camera called the electron bombardment
} ccd where accelerated electrons directly bombard a back thinned, peltier
} cooled ccd. The electrons are emitted from a photocathode for applications
} particularly in low light microscopy. With a full well capacity of 300,000
} electrons, I wonder if this approach could be used in direct exposure of the
} ccd to the electron beam.
} Shane

This certainly could be a significant improvement in CCD imaging.
Most of the width of the point spread function of current CCD camera
is due to photon spread in the scintillator, so presumably removing
the scintillator would allow digital images at resolutions very close
to the pixel size of the CCD chip.

I'm not familiar with the Hamamatsu camera, but I know that the
Gatan camera I currently use has a CCD with a full well capacity of
~500,000 electrons. In order for the Hamamatsu chip to work they
would have to find some way to reduce the electron/hole yield of the
incident fast electrons - maybe with a very thin CCD chip?

Paul Voyles


} K. Shane Collins
} Scientific Instrument Company
} 805.444.4953 cell
} 310.568.9188 office
} 310.568.9189 fax
}
}
} -----Original Message-----
} From: Paul Voyles [mailto:voyles-at-research.nj.nec.com]
} Sent: Friday, May 26, 2000 9:11 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: RE: new developments in imaging systems?
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Thu Jun 01 16:08:52 2000



From: Geoff McAuliffe :      mcauliff-at-UMDNJ.EDU
Date: Thu, 01 Jun 2000 11:27:44 -0400
Subject: Re: Resolving power: film vs digital

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Jeremy Sanderson wrote:

} Dear All,
}
} I've been following the thread on film vs digital.
} We all know that the resolving power of digital CCD
} faceplates is approaching that of conventional film
} emulsion, but isn't equivalent yet.
} Can someone remind me of the number of mega-pixels
} that a CCD will need to equate to ISO 100 print film,
} ISO 64 or ISO100 slide film and the highest resolving
} B&W film of all, Technical Pan at ISO 25 and ISO 100?
} If anyone out there can lead me through the logic and
} the maths, I'm sure others will also find it helpful.
} I'll repost a summary of the replies that I get.
}
} Regards, Jeremy Sanderson
}

About twenty-five million pixels in a 35mm Kodachrome slide is the
number I have heard from several sources, none of which I can remember now.

Geoff
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************




From daemon Thu Jun 01 16:08:52 2000



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Thu, 1 Jun 2000 09:23:53 -0600
Subject: RE: Film vs Digital

Contents Retrieved from Microscopy Listserver Archives
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No, it's not really a problem. It's been done with low density
microscopy all the time. Granted, there are some technical aspects to be
overcome, but (and I can only speak for ourselves) we have done that on
a number of microscopes. You are of course correct, that 10 images at
0.8 seconds take longer than 8 seconds as the image has to be
transferred, etc. BUT: that's what beam blankers are for. It is pretty
straightforward to take an image at 0.8 seconds, then blank the beam
very quickly before taking the next image. That way you get pretty close
to the 8 sec total exposure. If there is no beam blanker on the
microscope, in most cases it can be added.

I am not sure what you mean by "too sensitive". The cameras are usually
constructed so that 1 electron from the beam creates between a few tenth
to a few counts (these are all statistical data, of course). The well
width divided by this sensitivity then determines, how many primary
electrons are needed to fully expose one pixel. For example, if the well
width is 50,000 electrons and the sensitivity is 1 count/electron, one
needs 50,000 primary electrons to fill the well. This translates into
roughly a 0.4% statistical error.

} From a practical standpoint: You can take images with most cameras when
the exposure meter on the microscope reads a couple of seconds without
overexposing the camera. On the other hand, you can reduce the intensity
of the beam until you see single electron events.

The one area where CCD cameras may be too sensitive is diffraction. The
normally huge intensity in the transmitted beam often leads to
saturation. In CCDs this can lead to blooming (the intensity spills over
into neighboring pixels). This can be taken care of with special chips
that have anti-blooming features, but this usually has some other
drawbacks. Again, this can also be overcome somewhat with multiple
exposures. Film behaves more civilized here, as it simply stops
responding to the electrons, but this makes film more or less useless
for quantitative measurements of diffraction patterns. I have done
diffraction with CCDs many times and though it does require some
tweaking, one can get very good results from them.

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: jim [mailto:jim-at-proscitech.com.au]
Sent: Wednesday, May 31, 2000 9:37 PM
To: 'Mike Bode'; 'Microscopy-at-MSA.Microscopy.Com'


Yes, it could be done "in theory". Somebody would need to figure out the

software and perhaps modify the hardware. Then we would find that the
total
exposure of the specimen to the electron beam maybe a muliple of the
film's
exposure. Afterall, an 8 sec film exposure would not amount in digital
to
10x0.8, but we would require considerable time in between exposures.
Since the
problems in the discussed circumstances are specimen movement and beam
damage,
it seems that taking multiple exposures is a poor option.

Digital cameras are for some situation too sensitive to electron
exposure.
Cutting back on electrons is no option since its the electrons that form
the
image in the first instance.
Much easier in light microscopy . . . insert a neutral density filter.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com




From daemon Thu Jun 01 16:08:53 2000



From: Matt Ervin :      mervin-at-ARL.mil
Date: Thu, 1 Jun 2000 11:37:20 -0400
Subject: Re: SEM Stereo Image Analysis Software

Contents Retrieved from Microscopy Listserver Archives
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Jim-
I saw an ad a while back for such software. I have since moved on
to other things before I had a chance to obtain the demo software. Below is the
contact information I have for the company selling the stereo image analysis software.
Since this is an edited version of the message I received several months
ago, the terms and conditions may have changed.
If you do try this software, I, and I am sure the rest of the listserve would be very
interested in hearing how well it
works, as I do have an occational need for this capability.

Here is the information (edited) that I received from the company offering the software:
now the evaluation version of MeX is available.
The test period of MeX is limited to 6 weeks.

The evaluation version is delivered with a small database and the
complete analysis tools. You can process your own images without
restrictions.

We also offer to preinclude your SEM-images into the database of the
evaluation version. You just have to send us an email and we give you
detailed information on how you should capture your images.


If you have any questions, please do not hesitate to contact us.


Kind regards, Johann Schweiger

=====================================
= Dipl.-Ing.(FH) Johann Schweiger
= Sales
= Alicona GdbR
= Koch-Sternfeldstr. 5
= D-83471 Berchtesgaden/Germany
= Tel. ++49 8652 964205
= Fax. ++49 8652 964207

I hope that you find the above information helpful.
Sincerely,
Matthew H. Ervin, Ph.D.



From daemon Thu Jun 01 16:08:53 2000



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Thu, 1 Jun 2000 09:39:59 -0600
Subject: RE: SEM Stereo Image Analysis Software

Contents Retrieved from Microscopy Listserver Archives
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Jim,
you may want to check out our web site for the Stereo software. It has
some images and examples of stereo evaluations from SEM images. The
'Stereo' part is not a stand-alone software, but can be combines with
our analySIS Docu software for a stand-alone application.
Michael


Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================

Hello folks
Anyone aware of software capable of generating depth profiles from
digital SEM stereo pair images? I am aware of the Oxford ISIS
system/program, but wondered if other companies offer similar software
that would run 'stand-alone' in a PC and work with any digital files.
Any help or suggestions along these lines would be most appreciated.
Thanks,
Jim







From daemon Thu Jun 01 16:08:53 2000



From: Steve Chapman :      protrain-at-emcourses.com
Date: Thu, 1 Jun 2000 15:24:59 +0100
Subject: Wet Specimens

Contents Retrieved from Microscopy Listserver Archives
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Hi

Want to work with wet specimens?

Do not give up if you do not have an ESEM or LVSEM there may be hope yet?
Do you have a SEM with a rear manifold that connects directly to the DP and
do you have a BSE detector? Sorry but those SEM that have the pump directly
attached to the specimen chamber are no good for this procedure. AND I must
give all the credit to Viv Robinson who spawned this idea back in the 80's.

If you do have a manifold system you are in luck. Find a rubber bung that
will fit into the manifold at the rear of the specimen chamber. Drill (use
LN2) a 1/4" hole in the bung and then place it in the rear manifold. Switch
off or better still unplug your Everhart-Thornley detector (high voltage
plus poor vacuum = arcing!). Place you "wet" specimen in the microscope and
pump down. The bung will spoil the vacuum in the chamber for about 20
minutes or so and imaging with the BSE detector you will have your own LV
SEM.

To retain the moisture longer you may quench the specimen in LN2 before
putting it into the microscope. The frost will sublime away and you will be
able to watch what happens to the moisture etc.

We use this technique on all sorts of samples. A rubber bung is cheaper than
buying an LVSEM and the results, if you work quickly, are pretty good.

Try it?

Steve Chapman
Senior Consultant Protrain
For consultancy and professional training in EM world wide
Tel 44+ 1280 814774 Fax 814007
www.emcourses.com

Steve Chapman
Senior Consultant Protrain
For consultancy and professional training in EM world wide
Tel 44+ 1280 814774 Fax 814007
www.emcourses.com



From daemon Thu Jun 01 16:08:54 2000



From: Chris Jeffree :      cjeffree-at-srv0.bio.ed.ac.uk
Date: Thu, 1 Jun 2000 17:10:23 +0000
Subject: Re: Resolving power: film vs digital

Contents Retrieved from Microscopy Listserver Archives
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Is it just my problem, or isn't this all getting a little bit unfocussed!
As far as TEM is concerned, the CCD is not directly exposed to
the beam at all, so its effective resolution depends on the nature of
the system that presents the image to the CCD. The two
predominating technologies for transfer of the image to the CCD in
TEM cameras are a fibre-optic linkagage between a phosphor or
YAG scintillator and the CCD, or an optical coupling via a lens (for
example the excellent f1.2 50mm Zuiko macro lens by Olympus).
In both instances, the ultimate resolution of the system is probably
set by the electron sensor, which is the phosphor or YAG
scintillator. I suspect that fibre optic couplings probably degrade
that resolution, but I say that without reference to the facts, so
please correct me if I am mistaken. Optical coupling could in
principle project the spatial data recorded by the phosphor or YAG
to any desired magnification. It can thus be recorded by a CCD
using many pixels or few depending on the optical configuration.
So what do we mean by resolution in this context, when the
smallest object which can be imaged by a TEM can be projected
onto any desired quantity of CCD pixels?

To begin to answer Jeremy's question directly, we need to know
how much detail a Technical Pan negative can record. The figures
depend on processing technique and the test object luminance and
contrast, but the modulation transfer function figures published by
Kodak indicate that a spatial frequency in excess of 200 cycles per
mm is easily recordable. For a test object with contrast 100:1 they
quote 320 line pairs per mm. The CCD pixel spacing required to
achieve this feat would be 640 pixels per mm. That equates to a
requirement for 15360 x 23040 pixels to match the resolving power
of a 24x36mm Technical Pan exposure. That's 0.35 Giga pixels in
round numbers.

So what was that I heard about the death of silver imaging? I don't
think so. Not for a while yet.

Best wishes
Chris

} Dear All,
}
} I've been following the thread on film vs digital.
} We all know that the resolving power of digital CCD
} faceplates is approaching that of conventional film
} emulsion, but isn't equivalent yet.
} Can someone remind me of the number of mega-pixels
} that a CCD will need to equate to ISO 100 print film,
} ISO 64 or ISO100 slide film and the highest resolving
} B&W film of all, Technical Pan at ISO 25 and ISO 100?
} If anyone out there can lead me through the logic and
} the maths, I'm sure others will also find it helpful.
} I'll repost a summary of the replies that I get.
}
} Regards, Jeremy Sanderson
}
} __________________________________________________
} Do You Yahoo!?
} Send instant messages & get email alerts with Yahoo! Messenger.
} http://im.yahoo.com/
}


=====================================================================
DR CHRIS JEFFREE
BIOSEM - BIOLOGICAL SCIENCES EM FACILITY
UNIVERSITY OF EDINBURGH
Daniel Rutherford Building
King's Buildings, Mayfield Road
EDINBURGH, EH9 3JH, Scotland, UK
Tel. #44 131 650 5345
FAX. #44 131 650 6563
Mobile 0410 585 401
email c.jeffree-at-ed.ac.uk
SEM / TEM bookings sem-at-ed.ac.uk
=====================================================================



From daemon Thu Jun 01 16:08:54 2000



From: Mark Armogida :      mark_armogida-at-tedpella.com
Date: Thu, 01 Jun 2000 09:31:04 -0700
Subject: Re: curing Epon/Araldite with UV?

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We at Ted Pella Inc. have used our Pelco UVC2 UV Cryo Chamber to cure epoxies
but most of our UV curing has been with Acrylic resins. The literature we have
on Eponate Araldite does not show it to be UV curable but our chemist wouldn't
be surprised if it didn't accelerate the cure. The epoxy mixture should cure
overnight at 60 degrees C. We have a technical note on the use of
Epon-Araldite for embedding specimens and literature on our Cryo Chamber that
may help. I would be happy to fax them to you and/or have you talk with our
chemist.

Mark J Armogida
VP Engineering and Production
Ted Pella Inc.

Tamara Howard wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Greetings!
}
} Has anyone ever cured Epon-Araldite with UV? I was given a
} cobbled-together protocol by a non-microscopist post-doc; the protocol
} calls for a dry-ice, UV cure of Epon-Araldite. It says it can take from
} 3-7 days. I'm wondering if this is either a resin mix-up *or* the resin
} just set up all by itself (over that time frame I've seen it happen), just
} because it was a thin layer and left alone - nothing to do with the UV
} exposure. She has no ref. for this technique and isn't sure now where she
} got it. Sigh. I've only been able to find heat-cure protocols...anyone
} have any leads or thoughts on this UV thing?
}
} (Sorry about the cross-post for those of you on both of these servers)
}
} Thanks!
}
} Tamara (Planning to use the oven.....) Howard
} CSHL



From daemon Thu Jun 01 16:08:55 2000



From: Linda Boisselle :      THE-LAB-at-worldnet.att.net
Date: Thu, 01 Jun 2000 14:02:00 -0400
Subject: Seeking help

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I am seeking to speak to individuals who know about designing and
developing applications for indentification of rare cells in microscopic
biological preparations. Someone who knows how to optimize microscopy
autofocusing procedures. I am more interested in speaking to an
electrical engineer who is more into digital image processing. Can
anyone suggest what direction to take?

THE-LAB-at-att.net

Thanks.



From daemon Thu Jun 01 16:09:00 2000



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Thu, 1 Jun 2000 15:14:38 -0500
Subject: TEM: looking for phage

Contents Retrieved from Microscopy Listserver Archives
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Hi,

We are trying to help a client find and identify a bacteriophage. His
bacteria (Pasturella sp.) are plated out on agar and show well-defined clear
areas with sharp edges where the phage are supposed to be. So far, we have
taken carbon-coated grids and placed them gently onto the surface of the
clear areas, then lifted them off and negative-stained with PTA or uranyl
acetate. We have also run buffer across the clear areas, then pipetted it
onto the grids and stained it. A microbiologist who works with phage in
another lab has taken samples from the clear areas and concentrated them
down and we have stained those also.

So far we have found exactly two phage-like organisms in a total of about 10
grids. Not a stellar performance.

We figure the possibilities are: 1) the bacteria are being killed by
something other than phage; 2) we're looking for a particular type of phage
that may not be there, and we're just not seeing what's actually causing the
clear areas, or 3) for some reason we're just not getting the things
adhering to the grids, although we've used these methods successfully many
times before.

Does anyone else have any ideas that might help us out, especially on
technique? Our client is almost certain that phage are present. We just
can't find them.

Thanks in advance.

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/





From daemon Thu Jun 01 17:58:48 2000



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Thu, 1 Jun 2000 12:46:37 -1000 (HST)
Subject: SEM: immunolabeling

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One of our SEM users would like to label biofilms with lectins. Three of
them. At once. I have used gold conjugated to goat anti-biotin to label
biotinylated lectins for TEM in the past, so I'm hoping to follow the same
kind of procedure. I have not done any immunolabeling for SEM, although
our FESEM has been used for such. I would be grateful for any advice! If
he wants to triple label, what sizes of gold would be useful? If he wants
to quantify the three, what kinds of controls for labeling efficiency
should we run? All hints ahd tips gratefully accepted!

Mahalo,
Tina

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************



From daemon Thu Jun 01 17:58:49 2000



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Thu, 1 Jun 2000 12:50:40 -1000 (HST)
Subject: Confocal: gold enhancement

Contents Retrieved from Microscopy Listserver Archives
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I have not generally followed any discussion on gold enhancement for light
microscopy (photons? I don't do photons), but now I need to ask for
someone what people suggest for immunogold enhancement for confocal
microscopy. We have 10nm gold left over from TEM, and it would be useful
to use it for the confocal experiment. Yes, we're being cheap!

Mahalo,
Tina

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************



From daemon Thu Jun 01 20:59:23 2000



From: Gordon Couger :      gcouger-at-couger.com
Date: Thu, 1 Jun 2000 20:40:40 -0500
Subject: Re: Resolving power: film vs digital

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} From: "Chris Jeffree" {cjeffree-at-srv0.bio.ed.ac.uk}
To: "Jeremy Sanderson" {jb_sanderson-at-yahoo.com}
{snip}
} To begin to answer Jeremy's question directly, we need to know
} how much detail a Technical Pan negative can record. The figures
} depend on processing technique and the test object luminance and
} contrast, but the modulation transfer function figures published by
} Kodak indicate that a spatial frequency in excess of 200 cycles per
} mm is easily recordable. For a test object with contrast 100:1 they
} quote 320 line pairs per mm. The CCD pixel spacing required to
} achieve this feat would be 640 pixels per mm. That equates to a
} requirement for 15360 x 23040 pixels to match the resolving power
} of a 24x36mm Technical Pan exposure. That's 0.35 Giga pixels in
} round numbers.
}
At 16 bits this is per image this is 5,662,310,400 or 5.5 gig. I don't
think film is in any danger for a long time. We need at least 1 order
of magnitude for storage and 2 or 3 for processing. I remember
taking 4 days to process an image. And then work on the program and
trigger it again.

Gordon

Gordon Couger gcouger-at-couger.com

Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00








From daemon Fri Jun 02 08:39:48 2000



From: Jenichen :      Jenichen-at-proscan.de
Date: Fri, 02 Jun 2000 11:14:47 +0200
Subject: Re: Film vs Digital

Contents Retrieved from Microscopy Listserver Archives
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Hello all,
This is the last call for a second post-doc vacancy at the
University of Barcelona (Spain) to work in the frame of a TMR
programme concerning UV coatings. (More details below).

Since we are expecting the Mid-Term evaluation of the project,
the starting date has been delayed to next September, so we
have extended the deadline for applications.

Any one interested please reply directly to paqui-at-el.ub.es
and/or send applications and a CV by mail before 30 June 2000.

Kind regards

F. Peir—

**************************************************************************
Laboratory: Electronic Materials and Engineering, Department of
Electronics, University of Barcelona.

Duration: 12-months, starting September 2000.



Hi Jim, some comments to Your remarks within Your text:

jim schrieb:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
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} -----------------------------------------------------------------------.
}
} Yes, it could be done "in theory". Somebody would need to figure out the
} software and perhaps modify the hardware. Then we would find that the total
} exposure of the specimen to the electron beam maybe a muliple of the film's
} exposure. Afterall, an 8 sec film exposure would not amount in digital to
} 10x0.8, but we would require considerable time in between exposures.

Simply choose a CCD with higher readout performance (faster), but same quality.

} Since the
} problems in the discussed circumstances are specimen movement and beam damage,
} it seems that taking multiple exposures is a poor option.
}
} Digital cameras are for some situation too sensitive to electron exposure.

Not correct at all. The only thing is to choose the correct camera for the
application You work on. It is not complicated to make a digital system which
collects one count per incident electron to achieve the same signal to noise as in
the electron beam. This system will be less sensitive than normally sold systems
but the main advantage of digital systems that You see what You get remains and You
get instant results of Your work.
The only problem You have to solve for these systems is to use a not very sensitive
scintillator but a very high performance slow-scan CCD. So You get less visible
photons from Your electron which have to be converted in one digital count. But
these digital counts must be more than presently available to achieve a good
statistic. Thats the reason for our new 16bit CCD (dynamic up to 65536 digits) for
high performance TEM applications.

}
} Cutting back on electrons is no option since its the electrons that form the
} image in the first instance.
} Much easier in light microscopy . . . insert a neutral density filter.
} Cheers
} Jim Darley
} ProSciTech Microscopy PLUS
} PO Box 111, Thuringowa QLD 4817 Australia
} Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
} Great microscopy catalogue, 500 Links, MSDS, User Notes
} www.proscitech.com

--
Best regards / Mit freundlichen Gruessen
Dr. Frank Jenichen
Proscan elektronische Systeme GmbH
Tel.: +49 8195 999 -511 Fax: -512
mailto:Jenichen-at-proscan.de
------------------------------------------------------------
More information concerning our products
and services can be found on our website
http://www.proscan.de




From daemon Fri Jun 02 08:39:50 2000



From: Chris Jeffree :      cjeffree-at-srv0.bio.ed.ac.uk
Date: Fri, 2 Jun 2000 11:18:10 +0000
Subject: Re: Resolving power: film vs digital

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} About twenty-five million pixels in a 35mm Kodachrome slide is the
} number I have heard from several sources, none of which I can remember now.
}
} Geoff
Geoff
Analysing this a little further:
A 25 Mbyte image = 8.3 M pixels to cover 24x36mm or 864mm^2
this represents 98.2 pixels per mm, or 49 line pairs per mm. Pixel
size 10.2 um. Resolution about 50 line pairs per mm at best.

A 25M pixels image used to capture a 24x36mm Kodachrome
slide represents 28,935 pixels per mm^2 or 170 pixels per mm.
This is equivalent to a maximum resolution of 85 line pairs per mm,
which may be on the conservative side for Kodachrome. pixel size
= 5.88um. The image will be approx. 6120x4082 pixels, generating
a file size of approx 75Mb for a 24-bit (8+8+8bit) RGB image.

To record 120 line pairs per mm, which many top 35mm camera
lenses can achieve, a minimum of 240 pixels per mm are required,
each 4.2 um wide. This equates to 8640x5760 pixels for a
24x36mm frame = 50M-pixels or 50Mb in 8-bit greyscale, or 150Mb
in 24-bit RGB.

At 320 line pairs per mm (Technical Pan) the minimum required
640 pixels per mm is a pixel size of 1.56 um

Presumably for a light image the diffraction limited resolution is
approx 1/2 lambda which at 540nm is 0.27um.
So looking to the future of ultimate-performance CCDs, direct
recording of a diffraction limited light image projected onto the
sensor requires at the very least 3703 pixels per image mm or
13,717,421 pixels per mm^2 (greyscale 8-bit)

However, if we are doing light microscopy with an NA 1.4 x100
objective, how much resolving power do we need on CCD or film?

Data is at 0.27um resolution (lambda = 540nm). Let's round this to
0.3um. Magnification at 24x36mm film image is x100, so pixels
must be an absolute maximum of 30um wide to record the
significant data = 33.3 pixels per mm, equivalent to 800x1200
pixels to record the whole 35mm negative area. However, most
CCDs are much smaller than 35 mm frames, typically 1/3 inch. So
an 800x1200 pixel CCD at 8x12 mm, 1/3 of the linear dimensions
of a 35mm frame would use 9 pixels to record the smallest image
details. This is about right from the point of view of resolution, but
to record the whole 35mm frame we need about 2400x3600 pixels
on our CCD.

Note also that Technical Pan has (depending on the criterion used
to assess its performance) up to 10 times the resolving power
required to record all there is to see in a diffraction-limited LM
image made with a 100x NA 1.4 lens. So you can comfortably
afford to use a 60x NA 1.4 lens, thereby getting the same
resolution with a bigger field of view.

Many years ago, I took a photograph of a street scene using a
Canon 35mm SLR loaded with Kodak Recordak (I think this was a
single layer microfilm emulsion). Examined in a light microscope,
the image clearly, legibly recorded the brand-name of a child's
push chair. I tried to print this brand name using a DeVere point-
source enlarger with an image size of 20x30 inches produced with
a Schneider Componon lens, but was completely unable to
produce a legible image. The point I am making here is that the
combination of some high performance films, with high quality
lenses of the standard produced by the leading camera
manufacturers can record more detail on the film than you can
easily get back out by conventional printing. I suspect the same is
true of EM exposures.

So there is no contest - film beats CCDs for resolution hands
down. And you can process the image on the cheap. No money
goes to Intel or Microscoft, Adobe or Epson. But resolution is not
primarily what we buy CCDs for. We buy them primarily for instant
image capture in a format suitable for digital storage, digital
transmission quantitative data recording and image processing.

Chris

=====================================================================
DR CHRIS JEFFREE
BIOSEM - BIOLOGICAL SCIENCES EM FACILITY
UNIVERSITY OF EDINBURGH
Daniel Rutherford Building
King's Buildings, Mayfield Road
EDINBURGH, EH9 3JH, Scotland, UK
Tel. #44 131 650 5345
FAX. #44 131 650 6563
Mobile 0410 585 401
email c.jeffree-at-ed.ac.uk
SEM / TEM bookings sem-at-ed.ac.uk
=====================================================================



From daemon Fri Jun 02 08:39:55 2000



From: jim :      jim-at-proscitech.com.au
Date: Fri, 2 Jun 2000 21:15:36 +1000
Subject: RE: Resolving power: film vs digital

Contents Retrieved from Microscopy Listserver Archives
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Gordon's and Chris' contribution look very bleak for digital, but the
comparison is not really fair, as we should look at the practical aspects too.
The unaided eye resolves lines 0.1mm apart, so to see the full (200 black+200
white) 400+ lines/mm that may be recorded on TEM film we would need to enlarge
over 40x. This requires an enlarger with a very wide angle lens to print small
portions of the negative and it is technically difficult to so enlarge a whole
negative since a 4" negative becomes 160" or over 4m in size. That degree of
enlargement is not fully useful since in a well exposed TEM negative at just
under 30x electron noise becomes the problem, meaning not enough electrons
contributed to the image. So enlargements beyond 30x are empty- and provide no
further information. Truly not a serious problem.
The practical part is that few people ever find it useful to enlarge more than
15x and most TEM images reproduced are barely the size of the original
negative, however, they are enlarged from a smaller portion thereof.
For these most common applications, digitals with 10+ megabytes provide
excellent image details and tonal gradation. However, that size image can only
cover the equivalent of a small 35mm negative equivalent and does not allow
high enlargement or choosing of an adjacent field.
It appears that the best of both worlds is the use of conventional TEM
negatives for archiving and scanning those as required for printing.

It should be noted that this discussion concerns TEM. Digitals in SEM are less
daunting and it is not problematical to produce excellent digitals, comparable
with film.
Cheers
Jim Darley

ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Friday, June 02, 2000 11:41 AM, Gordon Couger [SMTP:gcouger-at-couger.com]
wrote:
}
}
} } From: "Chris Jeffree" {cjeffree-at-srv0.bio.ed.ac.uk}
} To: "Jeremy Sanderson" {jb_sanderson-at-yahoo.com}
} {snip}
} } To begin to answer Jeremy's question directly, we need to know
} } how much detail a Technical Pan negative can record. The figures
} } depend on processing technique and the test object luminance and
} } contrast, but the modulation transfer function figures published by
} } Kodak indicate that a spatial frequency in excess of 200 cycles per
} } mm is easily recordable. For a test object with contrast 100:1 they
} } quote 320 line pairs per mm. The CCD pixel spacing required to
} } achieve this feat would be 640 pixels per mm. That equates to a
} } requirement for 15360 x 23040 pixels to match the resolving power
} } of a 24x36mm Technical Pan exposure. That's 0.35 Giga pixels in
} } round numbers.
} }
} At 16 bits this is per image this is 5,662,310,400 or 5.5 gig. I don't
} think film is in any danger for a long time. We need at least 1 order
} of magnitude for storage and 2 or 3 for processing. I remember
} taking 4 days to process an image. And then work on the program and
} trigger it again.
}
} Gordon
}
} Gordon Couger gcouger-at-couger.com
}
} Stillwater, OK www.couger.com/gcouger
} 405 624-2855 GMT -6:00



From daemon Fri Jun 02 08:39:56 2000



From: jim :      jim-at-proscitech.com.au
Date: Fri, 2 Jun 2000 21:14:44 +1000
Subject: RE: Film vs Digital

Contents Retrieved from Microscopy Listserver Archives
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The world is full of possible solutions, but are they practical.?
To produce high-resolution, dark-field or any others TEM images that require
more electrons to form a clear image, Mike Bode would use multiple digital
exposures. The exposures could be layered and combined into one superior image.
This image would be made up of more pixel and is formed by more electrons and
so would be noise-free and hence could be further enlarged then otherwise
possible. Perhaps.
Beam blanking would largely save the specimen from beam damage and drift could
be compensated for by matching up the digitals. Great.
How much time is required between exposures to transfer a minimum 10mb image
per exposure? What would be the total time from focusing to the last exposure?
What about Z-drift in the interim requiring objective changes and what about
the total cost of this additional get-up. The mind boggles at a through focus
series.
When pushing the limits a piece of film seems more effective, cheaper and fa
ster.
Again, I don't doubt that there is now a large place for digital in TEM, but
its no panacea.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Friday, June 02, 2000 1:24 AM, Mike Bode [SMTP:mb-at-soft-imaging.com] wrote:
}
} No, it's not really a problem. It's been done with low density
} microscopy all the time. Granted, there are some technical aspects to be
} overcome, but (and I can only speak for ourselves) we have done that on
} a number of microscopes. You are of course correct, that 10 images at
} 0.8 seconds take longer than 8 seconds as the image has to be
} transferred, etc. BUT: that's what beam blankers are for. It is pretty
} straightforward to take an image at 0.8 seconds, then blank the beam
} very quickly before taking the next image. That way you get pretty close
} to the 8 sec total exposure. If there is no beam blanker on the
} microscope, in most cases it can be added.
}
} I am not sure what you mean by "too sensitive". The cameras are usually
} constructed so that 1 electron from the beam creates between a few tenth
} to a few counts (these are all statistical data, of course). The well
} width divided by this sensitivity then determines, how many primary
} electrons are needed to fully expose one pixel. For example, if the well
} width is 50,000 electrons and the sensitivity is 1 count/electron, one
} needs 50,000 primary electrons to fill the well. This translates into
} roughly a 0.4% statistical error.
}
} } From a practical standpoint: You can take images with most cameras when
} the exposure meter on the microscope reads a couple of seconds without
} overexposing the camera. On the other hand, you can reduce the intensity
} of the beam until you see single electron events.
}
} The one area where CCD cameras may be too sensitive is diffraction. The
} normally huge intensity in the transmitted beam often leads to
} saturation. In CCDs this can lead to blooming (the intensity spills over
} into neighboring pixels). This can be taken care of with special chips
} that have anti-blooming features, but this usually has some other
} drawbacks. Again, this can also be overcome somewhat with multiple
} exposures. Film behaves more civilized here, as it simply stops
} responding to the electrons, but this makes film more or less useless
} for quantitative measurements of diffraction patterns. I have done
} diffraction with CCDs many times and though it does require some
} tweaking, one can get very good results from them.
}
} Michael Bode, Ph.D.
} Soft Imaging System Corp.
} 1675 Carr St., #105N
} Lakewood, CO 80215
} ===================================
} phone: (888) FIND SIS
} (303) 234-9270
} fax: (303) 234-9271
} email: mailto:info-at-soft-imaging.com
} web: http://www.soft-imaging.com
} ===================================
}
}
}
} -----Original Message-----
} From: jim [mailto:jim-at-proscitech.com.au]
} Sent: Wednesday, May 31, 2000 9:37 PM
} To: 'Mike Bode'; 'Microscopy-at-MSA.Microscopy.Com'
} Subject: RE: Film vs Digital
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Yes, it could be done "in theory". Somebody would need to figure out the
}
} software and perhaps modify the hardware. Then we would find that the
} total
} exposure of the specimen to the electron beam maybe a muliple of the
} film's
} exposure. Afterall, an 8 sec film exposure would not amount in digital
} to
} 10x0.8, but we would require considerable time in between exposures.
} Since the
} problems in the discussed circumstances are specimen movement and beam
} damage,
} it seems that taking multiple exposures is a poor option.
}
} Digital cameras are for some situation too sensitive to electron
} exposure.
} Cutting back on electrons is no option since its the electrons that form
} the
} image in the first instance.
} Much easier in light microscopy . . . insert a neutral density filter.
} Cheers
} Jim Darley
} ProSciTech Microscopy PLUS
} PO Box 111, Thuringowa QLD 4817 Australia
} Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
} Great microscopy catalogue, 500 Links, MSDS, User Notes
} www.proscitech.com
}



From daemon Fri Jun 02 09:02:14 2000



From: Horst Neve :      neve-at-bafm.de
Date: Fri, 2 Jun 2000 08:40:59 -0500
Subject: TEM - Looking for phage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Randy,

if possible, select plates obtained from serial dilutions which show
confluent lysis (i.e., where plaques touch each other). They are a good
source for obtaining high-titer bacteriophage lysates (i.g., 10E10 / 10E11
plaque-forming units per ml). Harvest the phage by scraping the top agar
and transfer it into a test tube or equivalent. Rinse the plates with some
ml of phage diluent (i.g., 5 ml). Shake this supension containing phage,
host cells and agar for some while before spinning down the cells and the
agar. It is also a good idea to pick up a single plaque. Resuspend it in a
small volume of broth, and prepare a fresh lysate in some ml of broth with
fresh host cells. For rapid screening, we sometimes pipette a drop of
} buffer onto a plaque and float a piece of carbon film into the drop
directly from a mica support. After some minutes, we pick up the film with
a grid and do the routine negative staining. But you are right, the number
of phage particles is low in this case.
Best regards
Horst Neve

} At 15:14 01.06.00 -0500, you wrote:
} Hi,
}
} We are trying to help a client find and identify a bacteriophage. His
} bacteria (Pasturella sp.) are plated out on agar and show well-defined clear
} areas with sharp edges where the phage are supposed to be. So far, we have
} taken carbon-coated grids and placed them gently onto the surface of the
} clear areas, then lifted them off and negative-stained with PTA or uranyl
} acetate. We have also run buffer across the clear areas, then pipetted it
} onto the grids and stained it. A microbiologist who works with phage in
} another lab has taken samples from the clear areas and concentrated them
} down and we have stained those also.
}
} So far we have found exactly two phage-like organisms in a total of about 10
} grids. Not a stellar performance.
}
} Thanks in advance.
}
} Randy
}
} Randy Tindall
} EM Specialist
} Electron Microscopy Core Facility
} W122 Veterinary Medicine
} University of Missouri
} Columbia, MO 65211

****************************************************************************
****
Dr. Horst Neve
Institut fuer Mikrobiologie / Institute for Microbiology
Bundesanstalt fuer Milchforschung / Federal Dairy Research Centre
Postfach / P.O. Box 6069, D-24121 Kiel
Hermann-Weigmann-Str. 1, D-24103 Kiel
****************************************************************************
****
Tel. / Phone: +49 (0) 431 609 2343 {} Fax: +49 (0) 431 609 2306
E-mail: neve-at-bafm.de {} Internet: http://www.bafm.de
****************************************************************************
****




From daemon Fri Jun 02 09:02:14 2000



From: Horst Neve :      neve-at-bafm.de
Date: Fri, 2 Jun 2000 08:42:08 -0500
Subject: TEM - Looking for phage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Randy,

if possible, select plates obtained from serial dilutions which show
confluent lysis (i.e., where plaques touch each other). They are a good
source for obtaining high-titer bacteriophage lysates (i.g., 10E10 / 10E11
plaque-forming units per ml). Harvest the phage by scraping the top agar
and transfer it into a test tube or equivalent. Rinse the plates with some
ml of phage diluent (i.g., 5 ml). Shake this supension containing phage,
host cells and agar for some while before spinning down the cells and the
agar. It is also a good idea to pick up a single plaque. Resuspend it in a
small volume of broth, and prepare a fresh lysate in some ml of broth with
fresh host cells. For rapid screening, we sometimes pipette a drop of
} buffer onto a plaque and float a piece of carbon film into the drop
directly from a mica support. After some minutes, we pick up the film with
a grid and do the routine negative staining. But you are right, the number
of phage particles is low in this case.
Best regards
Horst Neve

} At 15:14 01.06.00 -0500, you wrote:
} Hi,
}
} We are trying to help a client find and identify a bacteriophage. His
} bacteria (Pasturella sp.) are plated out on agar and show well-defined clear
} areas with sharp edges where the phage are supposed to be. So far, we have
} taken carbon-coated grids and placed them gently onto the surface of the
} clear areas, then lifted them off and negative-stained with PTA or uranyl
} acetate. We have also run buffer across the clear areas, then pipetted it
} onto the grids and stained it. A microbiologist who works with phage in
} another lab has taken samples from the clear areas and concentrated them
} down and we have stained those also.
}
} So far we have found exactly two phage-like organisms in a total of about 10
} grids. Not a stellar performance.
}
} Thanks in advance.
}
} Randy
}
} Randy Tindall
} EM Specialist
} Electron Microscopy Core Facility
} W122 Veterinary Medicine
} University of Missouri
} Columbia, MO 65211

****************************************************************************
****
Dr. Horst Neve
Institut fuer Mikrobiologie / Institute for Microbiology
Bundesanstalt fuer Milchforschung / Federal Dairy Research Centre
Postfach / P.O. Box 6069, D-24121 Kiel
Hermann-Weigmann-Str. 1, D-24103 Kiel
****************************************************************************
****
Tel. / Phone: +49 (0) 431 609 2343 {} Fax: +49 (0) 431 609 2306
E-mail: neve-at-bafm.de {} Internet: http://www.bafm.de
****************************************************************************
****




From daemon Fri Jun 02 10:04:04 2000



From: Phillip Rutledge :      prutledge-at-ars.usda.gov
Date: Fri, 02 Jun 2000 08:23:17 -0600
Subject: Incubators

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Microbiologist experts:

I'm looking for a (preferably) bench top incubator. Non-water jacketed, not using B.O.D. bottles, unit needs to be using CFC free refrigeration system. Does anyone know of such a unit or where one can be purchased? Need to order one ASAP. We are a small research lab and the one we have is a monster (48"Hx46"Wx28"deep and weighs almost 500lbs.). I'd appreciate any info.

Thanks,

Phil Rutledge
prutledge-at-ars.usda.gov



From daemon Fri Jun 02 10:04:04 2000



From: Beverly_E_Maleeff-at-sbphrd.com
Date: Fri, 2 Jun 2000 10:50:05 -0400
Subject: M&M2000: Social Event Information

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The Microscopy & Microanalysis 2000 Local Arrangements Committee
is pleased to announce an additional social event at M&M2000---

TAKE ME OUT TO THE BALL GAME !!

Join us on Tuesday, August 15th as the
PHILADELPHIA PHILLIES meet the
ARIZONA DIAMONDBACKS at Veterans Stadium.
Game time is 7:35 PM.

For $32.50, you'll get:
- a ticket to the game (300 level, on the third base line)
- a $10.00 coupon, good toward purchases at all concession and souvenir stands
- round-trip transportation from the Convention Center to the stadium
on a luxury coach (buses leave at 6:00 PM; return to the convention
center immediately following the game)

A limited number of packages are still available. First come, first served.

Checks, in US$ only, payable to "Microscopy & Microanalysis 2000" can be mailed
to:

Bev Maleeff
Treasurer, M&M2000 LAC
c/o SmithKline Beecham Pharmaceuticals
Mail Code UE 0462
709 Swedeland Road
King of Prussia, PA 19406

Checks only, please. No credit cards accepted.
Your cancelled check will serve as your receipt.

Tickets will be distributed at the M&M2000 Hospitality Booth
on Monday and Tuesday, August 14th & 15th.

Don't miss out on the fun!!!

Further information about this and other M&M2000 events can be found on our
website:
http://www.msa.microscopy.com/~mm2000/

See you in August!!

Bev Maleeff
M&M2000 LAC




From daemon Fri Jun 02 10:24:01 2000



From: William McManus :      billemac-at-biology.usu.edu
Date: Fri, 2 Jun 2000 09:16:34 -0600
Subject: TEM: Freeze Fracture help

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Date sent: Fri, 02 Jun 2000 11:03:03 -0400
To: c.jeffree-at-ed.ac.uk
} From: joe fu {jofu-at-nist.gov}


I have a researcher here at USU who would like to have some freeze
fracture preps made of lysosome. Is there anyone out there who is
currently doing FF preps and would be willing to assist us? I can image
the preps here in Logan.

William McManus
Supervisor
Electron Microscopy Facility
Department of Biology
Utah State University
Logan UT 84322-5305

billEMac-at-cc.usu.edu
435-797-1920



From daemon Fri Jun 02 10:43:58 2000



From: John J. Bozzola :      bozzola-at-siu.edu
Date: Fri, 2 Jun 2000 10:43:42 -0500
Subject: Re: TEM: looking for phage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Rando,

Having worked with a variety of phages (Candida, Streptococcus, E.
coli) I can tell you that finding phages from an agar plaque is very
difficult--as you have determined. The best way is to do a liquid
culture and then high speed followed by ultracentrifugation to
concentrate the particles. FYI, as I recall, on a 200 mesh grid each
virus particle is roughly equivalent to 3.4 x 10E6 vp/ml. So, you
need a lot of particles to even find one of them using this approach.

JB
####################################################################
John J. Bozzola, Ph.D., Director
Micro-Imaging and Analysis Center
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
####################################################################



From daemon Fri Jun 02 11:53:48 2000



From: Ruth Yamawaki :      yamawaki-at-stanford.edu
Date: Fri, 2 Jun 2000 09:48:03 -0700
Subject: Outside electrical near the EM lab

Contents Retrieved from Microscopy Listserver Archives
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My EM lab is going to be moved and I have just seen the plans. Apparently
two large equipment room are going to be built, one right across the hall
and the other two doors down. I have been told that the equipment room will
house -80 freezers, centrifuges, etc. Does anyone out there have experience
with this type of equipment near their TEM? I will not be able to test the
room before the move because nothing has been built yet and we all scheduled
to move in at the same time. I am hoping to have some influence on the
architects now. They have been told (and I shall keep reminding them) that
no electrical circuits are to be passed around the EM room.

Thanks.

Ruth

**********************************************
Ruth Yamawaki
Department of Comparative Medicine
Building 330, Quad 7, RAF-1
Stanford CA 94305
(650) 723-3457
**********************************************



From daemon Fri Jun 02 12:13:44 2000



From: Fortner, Jeffrey A. :      fortner-at-cmt.anl.gov
Date: Fri, 2 Jun 2000 14:36:59 -0500
Subject: RE: Outside electrical near the EM lab

Contents Retrieved from Microscopy Listserver Archives
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Well, let's see:

Jim wrote:

Mike Bode would use multiple digital
exposures. The exposures could be layered and combined into one superior
image.
This image would be made up of more pixel and is formed by more
electrons and
so would be noise-free and hence could be further enlarged then
otherwise
possible. Perhaps.

No, I did not talk about further enlargements. All I wanted to say is,
that a more noise-free image can be achieved by adding multiple images,
and that this also to some extent helps with drift of the sample during
acquisition.

Jim wrote:

How much time is required between exposures to transfer a minimum 10mb
image
per exposure?

How did you arrive at 10 MB? A 1280x1024 image with 16 bit pixel
information is about 2.5 MB (uncompressed). We acquire about 10 of those
per second and transfer them across the PC bus to the display. Putting
them on them into Memory might add a few tenth of a second. Writing to
HD can be done after all images are acquired.

Jim wrote:

What would be the total time from focusing to the last exposure?
What about Z-drift in the interim requiring objective changes

Why would we have to worry about that, if we don't have to worry about
that when taking the image on film? In fact, we could take care of this
by looking at the image between exposures and correct for z-drift.
However, as you said, that would add to the overall time and exposure. I
was comparing a normal dark field image taken on film at 8 seconds with
acquiring the same image on a "too sensitive" CCD camera by adding up 10
consecutive .8 second images. Why would the sample drift (in x, y or z)
substantially more in 8+delta seconds than in 8?

Jim wrote:

what about the total cost of this additional get-up

That of course depends on the microscope and there is no general answer.
For example on a LEO 912 I believe the blanker is standard. The
additional cost to use an acquisition scheme like this with our software
is $0 plus perhaps a bit of time to write a small macro. On other
microscopes one might have to add a beam blanker and perhaps a control
mechanism for the beam blanker. But I would guess, that this cost is not
very high. All modern microscopes are computer controller anyway, so it
is most likely just a control command that needs to be sent to the
microscope over a serial port if the beam blanker is installed. Piece of
cake.

Jim wrote:

The mind boggles at a through focus series.

You're right here. But I don't think we were talking about through-focus
series. Incidentally, we do through-focus series on light microscopes
and reconstruction routinely. Takes a few images at different focus (or
for a light microscope: stage) settings. The rest is done off-line.
Takes maybe a couple of minutes for about 20 images of about 1kx1k. I
agree that TEM is different here and much more complicated due to the
complicated Contrast Transfer Function. However, this could in principle
be sorted out.

Jim wrote:

Again, I don't doubt that there is now a large place for digital in TEM,
but
its no panacea.

I also agree with you on that one. But using the additional computer
possibilities of digital imaging might take you further than expected.

Michael


Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: jim [mailto:jim-at-proscitech.com.au]
Sent: Friday, June 02, 2000 5:15 AM
To: 'Mike Bode'; 'Microscopy-at-MSA.Microscopy.Com'
Cc: 'jim-at-proscitech.com.au'


This can be hard to predict. Once we were having trouble with rather huge
(100 eV) energy fluctuations in our GIF 200 energy filter. We traced the
source to an adjoining room filled with constant-temperature ovens, fans,
and other high-current equipment. The unlikely source finally turned out to
be a $50 hot plate-stirrer!

Keeping the AC circuitry from running near the lab is a very good
start- I know this has devastated other labs. Ask for your own independent
electrical ground for the 'scope, and that all electrical circuits to your
lab remain independent of other rooms. I doubt that the equipment you
describe will be a big problem as long as you don't share AC circuits,
ground, or a common wall.
Good luck.


"The chief source of problems is solutions."
-Eric Sevareid

...........................................................................
......................................................
Jeffrey A. Fortner
Argonne National Laboratory
CMT/205
9700 S. Cass Avenue
Argonne, IL 60439-4837

(630) 252-5594 (voice)
(630) 252-4771 (fax)





} ----------
} From: Ruth Yamawaki
} Sent: Friday, June 2, 2000 11:48 AM
} To: 'Microscopy-at-sparc5.microscopy.com'
} Subject: Outside electrical near the EM lab
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} My EM lab is going to be moved and I have just seen the plans. Apparently
} two large equipment room are going to be built, one right across the hall
} and the other two doors down. I have been told that the equipment room
} will
} house -80 freezers, centrifuges, etc. Does anyone out there have
} experience
} with this type of equipment near their TEM? I will not be able to test
} the
} room before the move because nothing has been built yet and we all
} scheduled
} to move in at the same time. I am hoping to have some influence on the
} architects now. They have been told (and I shall keep reminding them)
} that
} no electrical circuits are to be passed around the EM room.
}
} Thanks.
}
} Ruth
}
} **********************************************
} Ruth Yamawaki
} Department of Comparative Medicine
} Building 330, Quad 7, RAF-1
} Stanford CA 94305
} (650) 723-3457
} **********************************************
}
}



From daemon Fri Jun 02 16:23:08 2000



From: Suzannah Mayo :      smayo-at-southernmicro.com
Date: Fri, 02 Jun 2000 17:02:06 -0400
Subject: Re: TEM - Looking for phage

Contents Retrieved from Microscopy Listserver Archives
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There is a standard technique for phage isolation and purification in Maniatis.
I have found it to work better than any kit and can be modified accordingly.

Horst Neve wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Randy,
}
} if possible, select plates obtained from serial dilutions which show
} confluent lysis (i.e., where plaques touch each other). They are a good
} source for obtaining high-titer bacteriophage lysates (i.g., 10E10 / 10E11
} plaque-forming units per ml). Harvest the phage by scraping the top agar
} and transfer it into a test tube or equivalent. Rinse the plates with some
} ml of phage diluent (i.g., 5 ml). Shake this supension containing phage,
} host cells and agar for some while before spinning down the cells and the
} agar. It is also a good idea to pick up a single plaque. Resuspend it in a
} small volume of broth, and prepare a fresh lysate in some ml of broth with
} fresh host cells. For rapid screening, we sometimes pipette a drop of
} } buffer onto a plaque and float a piece of carbon film into the drop
} directly from a mica support. After some minutes, we pick up the film with
} a grid and do the routine negative staining. But you are right, the number
} of phage particles is low in this case.
} Best regards
} Horst Neve
}
} } At 15:14 01.06.00 -0500, you wrote:
} } Hi,
} }
} } We are trying to help a client find and identify a bacteriophage. His
} } bacteria (Pasturella sp.) are plated out on agar and show well-defined clear
} } areas with sharp edges where the phage are supposed to be. So far, we have
} } taken carbon-coated grids and placed them gently onto the surface of the
} } clear areas, then lifted them off and negative-stained with PTA or uranyl
} } acetate. We have also run buffer across the clear areas, then pipetted it
} } onto the grids and stained it. A microbiologist who works with phage in
} } another lab has taken samples from the clear areas and concentrated them
} } down and we have stained those also.
} }
} } So far we have found exactly two phage-like organisms in a total of about 10
} } grids. Not a stellar performance.
} }
} } Thanks in advance.
} }
} } Randy
} }
} } Randy Tindall
} } EM Specialist
} } Electron Microscopy Core Facility
} } W122 Veterinary Medicine
} } University of Missouri
} } Columbia, MO 65211
}
} ****************************************************************************
} ****
} Dr. Horst Neve
} Institut fuer Mikrobiologie / Institute for Microbiology
} Bundesanstalt fuer Milchforschung / Federal Dairy Research Centre
} Postfach / P.O. Box 6069, D-24121 Kiel
} Hermann-Weigmann-Str. 1, D-24103 Kiel
} ****************************************************************************
} ****
} Tel. / Phone: +49 (0) 431 609 2343 {} Fax: +49 (0) 431 609 2306
} E-mail: neve-at-bafm.de {} Internet: http://www.bafm.de
} ****************************************************************************
} ****



From daemon Fri Jun 02 16:53:03 2000



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Fri, 02 Jun 2000 14:43:49 -0700
Subject: Fwd: RE: Film vs Digital

Contents Retrieved from Microscopy Listserver Archives
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Dear Jim

I spent about two years trying to make good pictures of my NanoGold labeled
protein-DNA complexes. Doing this job I find two main problems: the
sample is unstable under the beam as any biological sample; NanoGold is
much more bright than protein core in dark-field. I find that it is
impossible to record equally perfect signals from NanoGold and protein core
because of short dynamic range for SO-163 film, I believe. I was trying to
make two pictures with different exposure, but it is tricky: in dark-field
mode the automatic exposure meter usually does not work and we have to set
exposure manually, in this case it is difficult to get "right" exposure
time in the right moment, you know. Again, because of sample's short life
under the beam, it is impossible to make a couple pictures at the different
conditions sometime. Keep in mind, please, that to change the film in the
microscope it takes about 10 seconds. Your idea about increasing
signal-noise ratio by collecting more electrons is bright but not
practical. For biological samples (I am talking about non-fixed,
non-stained samples of proteins, DNA or RNA-protein complexes etc) the
electron damage is a huge problem. People are trying to solve it in
different ways. Some using cryo temperature (to stabilize the biological
structure). I was using freeze-drying (I find that freeze-dried samples
are more stable under the beam). But in any case we have deal with very
unstable samples and must to do everything to decrease (not increase as you
recommended) electron dose. Drift is a second big problem for such
application: to increase signal-noise ratio we have to use very thin
support films. Images obtained at such conditions are noisy and in most
cases we have to use image analysis tools to extract the data. It means
that we have to digitize our images anyway. In such situation digital
camera may help. As you, probably, remember I was a person who initiates
this discussion. I think this discussion was very useful for many of us
who are not friendly with digital camera's techniques. We understand the
limitations of the modern digital cameras better now. I would like to say
thank you everybody who was involved in this discussion. There is some
conclusions I make for myself from discussion:


- Film is still cheap and universal material for recording and
storage EM images, sorry CCD.
- CCD TEM camera should not substitute film. Film and camera should
work all together improving the flexibility of the TEM system. For this
reason I will chose side-mount camera if will have money for it.
- For cell-biology (thin sections) where the resolution of the sample
is about 3 nm CCD camera may do a good job allowing users to make a huge
number of pictures (cell-biology guys love it), instantly view and
catalogize them.
- Sometime the digital camera may help in area of high-resolution
(relatively high, guys) EM when image will be digitized anyway. The major
limitation here is small area of view (we need a lot of particles for image
analysis sometime), but you could make the set of overlapping pictures and
digitally combine it. I love, also, Mike Bode idea to make a few very
short exposure pictures and combine it digitally later to reduce noise. The
relatively big size of CCD's pixels is a real problem too.
- CCD camera is expensive "toy". I am not sure that the benefits from
using it will compensate astronomical price, actually the third of the
electron microscope value ($70000 is it 1/3 of microscope's price on
current market?). Currently, I would recognize the CCD TEM camera as funny
"attachment" which may be useful if you rich enough to spend money on it
(it mean, that you have everything else in your EM lab plus some extra
$70000 for the fun playing with digital "toy").
- TEM CCD cameras are under extensive development now. Today's
camera will be replaced on the new model (read better, faster, what else?)
next year. Next year's camera will be easily forgotten next after the next
year and so on... Each new camera will be better that previous one... CCD
TEM camera it is not a good investment of money, I think.
- We should keep in mind that many companies charged extra 3-4K$ for
the installation and training (it is mandatory for Gatan for instance) and
you, probably, have to buy service contract on it even if you have service
contract on the microscope (JEOL's service contract on microscope do not
cover the CCD camera even if you buy it from JEOL).

Best regard, Sergey




} Date: Fri, 02 Jun 2000 21:14:44 +1000
} From: jim {jim-at-proscitech.com.au}
} Subject: RE: Film vs Digital
} To: 'Mike Bode' {mb-at-Soft-Imaging.com} ,
} "'Microscopy-at-MSA.Microscopy.Com'" {Microscopy-at-sparc5.microscopy.com}
} Cc: "'jim-at-proscitech.com.au'" {jim-at-proscitech.com.au}
} Reply-to: "jim-at-proscitech.com.au" {jim-at-proscitech.com.au}
} Organization: ProSciTech
} X-Mailer: Microsoft Internet E-mail/MAPI - 8.0.0.4211
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu
http://www.bol.ucla.edu/~sryazant




From daemon Sat Jun 03 08:18:09 2000



From: Jonathan Barnard :      Jonathan.Barnard-at-angstrom.uu.se
Date: Sat, 03 Jun 2000 14:19:19 +0200
Subject: TEM:beam damage vs voltage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Chris,

I agree with most of your statements, and I don't think that anybody
would argue the point, that a raw CCD chip has a better resolution that
film. As you pointed out, film can have a very small grain size ( {1
micron) and CCD chips usually have a few microns pixel size.

But that is not the end of the story. An optical system normally
consists of more than a chip or a sheet of film. The question is, can I
get the resolution I want or need. And here the situation is not as
simple. For example: I used to do high-resolution TEM. What you do there
is operate the microscope at optimum condition, then take a picture (on
film). You then go to the darkroom and develop prints by blowing up the
negative 10, 20 or even more times. When you then look at the images,
you can usually see the grains of the film (especially if you then scan
those into a copmputer). So, we are working at the resolution limit of
the film, and according to your postings, we should not be able to see
anything on a CCD. But that's not true. By using some geometrical
properties (the camera sits further down in the column and sees an
already enlarged image) and a tapered fiber-optic, you can acquire just
as good and better images of the same structure. Both images are limited
by the point resolution of the TEM and not by the Film or CCD
resolution.

What I am trying to say, and what I have said before is, that film is
definitely better when it comes to maximizing the product of resolution
AND field of view. However, if we can trade one for the other, I believe
in most cases you get better results from a CCD.

Having said that and looking at a print of Ansel Adams, I am glad there
is film, though!!

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Chris Jeffree [mailto:cjeffree-at-srv0.bio.ed.ac.uk]
Sent: Friday, June 02, 2000 10:16 AM
To: joe fu
Cc: microscopy-at-sparc5.microscopy.com


Date sent: Fri, 02 Jun 2000 11:03:03 -0400
To: c.jeffree-at-ed.ac.uk
} From: joe fu {jofu-at-nist.gov}


Conventional wisdom said that when it came to using TEM for biological
specimens (or beam sensitive specimens) it is always best to use a lower
accelerating voltage. Many lower voltage microscopes (V { 120 kV) were sold
on this assumption.
However is this assumption true? At the present there are many research
establishments, buying TEMs for biological use, who are using 200 to 300 kV
beams. So obviously there has been a shift in the conventional way of
thinking. Being materials based I am not sure what the status quo is in
biological TEM.
I know that the ratio of inelastic to elastic scattering cross sections is
greater than one for the elements Z {12, but how does this change as the
beam energy increases? What are your experiences? This is really just
academic curiosity by the way, but I am sure many of you would appreciate
the question.
********************************************************
Dr Jonathan Barnard

Analytical Materials Physics
The Angstrom Laboratory, Uppsala University
P O Box 534, SE-751 21 Uppsala, Sweden
Phone: +46-(0)18-4716838 Fax: +46-(0)18-500131
Phone: Microscope room +46 18 471 6365
http://www.angstrom.uu.se/analytical/home.html
********************************************************



From daemon Sun Jun 04 10:18:19 2000



From: Larry Allard :      l2a-at-ornl.gov
Date: Sat, 03 Jun 2000 11:46:51 -0400
Subject: Sergey's comments Re: Film vs Digital

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Sergey writes:

} } - CCD camera is expensive "toy". I am not sure that the
} } benefits from using it will compensate astronomical price, actually
} } the third of the electron microscope value ($70000 is it 1/3 of
} } microscope's price on current market?). Currently, I would
} } recognize the CCD TEM camera as funny "attachment" which may be
} } useful if you rich enough to spend money on it (it mean, that you
} } have everything else in your EM lab plus some extra $70000 for the
} } fun playing with digital "toy").
- TEM CCD cameras are under extensive development now. Today's
camera will be replaced on the new model (read better, faster, what
else?) next year. Next year's camera will be easily forgotten next
after the next year and so on... Each new camera will be better that
previous one... CCD TEM camera it is not a good investment of money,
I think. { {


Sergey:

I appreciate your post, but...


1. Remember that "time is money"...there is no question that there
is a value in the nearly instantaneous result that derives from the
use of digital imaging systems, particular on TEMs. Also, the
ability to rapidly process and analyze your images to determine if
they are "keepers" is priceless, IMO.

2. In our large, national multi-user facility we have been
digital-only since about 1993 or so. We have no darkrooms for plate
loading or enlarging. The only "chemicals" we handle in the entire
photographic process are toner cartridges for our laser printers, or
equivalent media for dye sub printers etc. In most instances, we
process our images electronically all the way through to the final
presentation. This includes preparation of PowerPoint slides for
digital projection for talks, to sending full papers out for
publication on disks or via e-mail attachments. No user has *ever*
complained about the non-availability of film, or about the "lack of
resolution of CCD images" compared to film. On the contrary, we have
users that travel to our laboratory specifically to do work on our
instruments because of the availability of digital imaging, when the
same instruments in their own laboratories are not equipped with
digital cameras.

3. Digital camera systems also provide the capability to conduct
research with outside colleagues live-time via Telepresence
Microscopy methods. This is a rapidly developing capability in our
field that just cannot be done (on a TEM at least) if your
microsocope only uses film for imaging.

4. The 1k x 1k Gatan CCD camera on our Hitachi HF-2000 was purchased
for about $100K in 1993, and was upgraded about 5 years ago to a
multi-scan capability. It is still functioning perfectly today, as
is a similar camera on our JEOL 4000EX TEM. The very newest CCD
cameras might offer faster read-out times, but there has been no
order-of-magnitude improvement in capabilities to make our older
camera so obsolete that we desire to purchase a new one. The point
is that, unlike desk-top computers, the *expensive* digital camera
you purchase today will definitely *not* be obsolete next year...

5. A CCD TEM camera is probably the *best* investment anyone could
make to advance the research throughput in their microscopy facility.

All just MHO...

Larry

Dr. Lawrence F. Allard
Senior Research Staff Member
High Temperature Materials Laboratory
Oak Ridge National Laboratory
1 Bethel Valley Road
Bldg. 4515, MS 6064
PO Box 2008
Oak Ridge, TN 37831-6064

865-574-4981
865-576-5413 Fax
allardlfjr-at-ornl.gov



From daemon Sun Jun 04 10:18:22 2000



From: Gordon Couger :      gcouger-at-couger.com
Date: Sat, 3 Jun 2000 12:53:11 -0500
Subject: Re: Resolving power: film vs digital

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} From: "Mike Bode" {mb-at-Soft-Imaging.com}
To: "'Microscopy-at-MSA.Microscopy.Com'" {Microscopy-at-sparc5.microscopy.com}
} Chris,
}
} I agree with most of your statements, and I don't think that anybody
} would argue the point, that a raw CCD chip has a better resolution that
} film. As you pointed out, film can have a very small grain size ( {1
} micron) and CCD chips usually have a few microns pixel size.
}
} But that is not the end of the story. An optical system normally
} consists of more than a chip or a sheet of film. The question is, can I
} get the resolution I want or need. And here the situation is not as
} simple. For example: I used to do high-resolution TEM. What you do there
} is operate the microscope at optimum condition, then take a picture (on
} film). You then go to the darkroom and develop prints by blowing up the
} negative 10, 20 or even more times. When you then look at the images,
} you can usually see the grains of the film (especially if you then scan
} those into a computer). So, we are working at the resolution limit of
} the film, and according to your postings, we should not be able to see
} anything on a CCD. But that's not true. By using some geometrical
} properties (the camera sits further down in the column and sees an
} already enlarged image) and a tapered fiber-optic, you can acquire just
} as good and better images of the same structure. Both images are limited
} by the point resolution of the TEM and not by the Film or CCD
} resolution.c


For a scanning device be it light, electrons, x-rays or gamma rays is a CCD
array the best way to capture the image. My experience has been with gamma
rays and to some extent x rays. We have not found a good way to focus gamma
rays so we use a crystal that emitted light and a photomultiplier tube and
we
got good images. The resolution depended on the aperture of the gamma ray
source and was pretty large.

If you are scanning a sample wiht an electron beam the same principle should
work. You would not need long dwell times but build the images out of
multiple
scans.

If you are scanning the sample the array of pixels seems redundant to me.
Also a photomultiplier tube and crystal are a great deal more sensitive than
CCD arrays and have a good deal more dynamic range.

The time to make a film image is allows going to be less than making a
digital image.
The ability to immediately see the digital image is a very handy thing.
There are ways
to develop B&W film that you can see your image in 5 minutes. For 4 X 5
images I am
using BZT tubes that are tubs with a inter circumference of a little over 4
inches. You
put the file and developer in the tube and put the tub in a pan of water and
spin the tube
in the water. The stop bath and fixing can be carried out in room light and
it results in
the most even development I have ever seen.

For archival storage I don't think 35 mm film can be beat for cost and
resolution. Unfortunately
you can't see the results until later occasional making it necessary to
reshoot the session if
you rely on film alone.

If you really need long term archival storage you might consider using a
slide printer
for a computer to print the digital images. We know the life of silver film
is longer then
100 years. For the LM folks that are using color images you could use
PhotoShop to
do a color separation and print out three negatives on black and white for
storage.

Hard drives And CD ROMS have come a long way but I loose about 20% of my
hard
drive storage a year and loose an occasional CDROM to scratches. Film would
survive
the scratches with a minimal loss of information insted loosing the whole
CDROM.

Gordon

Gordon Couger gcouger-at-couger.com

Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00






From daemon Sun Jun 04 10:18:27 2000



From: STANSMAN-at-aol.com
Date: Sat, 3 Jun 2000 17:21:51 EDT
Subject: Re: Resolving power: film vs digital Commercial Post

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello all,

Please be aware that this is a commercial post that may be of interest to
some of the list members.


Digital light microscope cameras are now available that use a highly touted
CCD detector: Color or monochrome; fast interline transfer, high sensitivity,
low noise; 6.7 micron square pixel; 2/3 inch 1.3 Million chip; PCI bus frame
transfer. This detector is ideal to use in light microscopy applications.
Now precision technology and fast high capacity cheap computers allow this
detector to be "Micro Stepped" providing digital images of up to 12 million
pixels to be captured very quickly and providing file sizes of up to 35 MB in
24 bit color images. "Inter Pixel Stepping" technology will allow a
considerably less expensive approach to digital imaging that can now approach
film resolving capability in a light microscope.

Hats of to Dr. Jeffree's explanation on optical resolution as it relates to
light microscopy applications. With high pixel density capable digital
cameras, now lower magnification, low NA (lower resolving power) objectives
can be used to acquire matching digital resolution images of wide fields of
view.

Information on Nikon's Instrument Division "Digital Eclipse" family of
digital cameras for microscopy including the new DXM1200 digital color camera
will soon be available on our web site www.nikonusa.com or you can go to
our Dealer Locator at
http://www.nikonusa.com/corpinfo/dealers/dealerSearch.cfm and ask your
local Nikon authorized microscope dealer for further information.

Best Regards,

Stan Schwartz
Manager, BioSciences Dept.
Nikon Inc Instrument Division
1300 Walt Whitman Rd.
Melville, NY 11747
631-547-8500
631-547-4033 Fax
Schwartz-at-nikonincmail.com
www.nikonusa.com


Earlier post
=============================================================
Geoff
Analysing this a little further:
A 25 Mbyte image = 8.3 M pixels to cover 24x36mm or 864mm^2
this represents 98.2 pixels per mm, or 49 line pairs per mm. Pixel
size 10.2 um. Resolution about 50 line pairs per mm at best.

A 25M pixels image used to capture a 24x36mm Kodachrome
slide represents 28,935 pixels per mm^2 or 170 pixels per mm.
This is equivalent to a maximum resolution of 85 line pairs per mm,
which may be on the conservative side for Kodachrome. pixel size
= 5.88um. The image will be approx. 6120x4082 pixels, generating
a file size of approx 75Mb for a 24-bit (8+8+8bit) RGB image.

To record 120 line pairs per mm, which many top 35mm camera
lenses can achieve, a minimum of 240 pixels per mm are required,
each 4.2 um wide. This equates to 8640x5760 pixels for a
24x36mm frame = 50M-pixels or 50Mb in 8-bit greyscale, or 150Mb
in 24-bit RGB.

At 320 line pairs per mm (Technical Pan) the minimum required
640 pixels per mm is a pixel size of 1.56 um

Presumably for a light image the diffraction limited resolution is
approx 1/2 lambda which at 540nm is 0.27um.
So looking to the future of ultimate-performance CCDs, direct
recording of a diffraction limited light image projected onto the
sensor requires at the very least 3703 pixels per image mm or
13,717,421 pixels per mm^2 (greyscale 8-bit)

However, if we are doing light microscopy with an NA 1.4 x100
objective, how much resolving power do we need on CCD or film?

Data is at 0.27um resolution (lambda = 540nm). Let's round this to
0.3um. Magnification at 24x36mm film image is x100, so pixels
must be an absolute maximum of 30um wide to record the
significant data = 33.3 pixels per mm, equivalent to 800x1200
pixels to record the whole 35mm negative area. However, most
CCDs are much smaller than 35 mm frames, typically 1/3 inch. So
an 800x1200 pixel CCD at 8x12 mm, 1/3 of the linear dimensions
of a 35mm frame would use 9 pixels to record the smallest image
details. This is about right from the point of view of resolution, but
to record the whole 35mm frame we need about 2400x3600 pixels
on our CCD.

Note also that Technical Pan has (depending on the criterion used
to assess its performance) up to 10 times the resolving power
required to record all there is to see in a diffraction-limited LM
image made with a 100x NA 1.4 lens. So you can comfortably
afford to use a 60x NA 1.4 lens, thereby getting the same
resolution with a bigger field of view.

Many years ago, I took a photograph of a street scene using a
Canon 35mm SLR loaded with Kodak Recordak (I think this was a
single layer microfilm emulsion). Examined in a light microscope,
the image clearly, legibly recorded the brand-name of a child's
push chair. I tried to print this brand name using a DeVere point-
source enlarger with an image size of 20x30 inches produced with
a Schneider Componon lens, but was completely unable to
produce a legible image. The point I am making here is that the
combination of some high performance films, with high quality
lenses of the standard produced by the leading camera
manufacturers can record more detail on the film than you can
easily get back out by conventional printing. I suspect the same is
true of EM exposures.

So there is no contest - film beats CCDs for resolution hands
down. And you can process the image on the cheap. No money
goes to Intel or Microscoft, Adobe or Epson. But resolution is not
primarily what we buy CCDs for. We buy them primarily for instant
image capture in a format suitable for digital storage, digital
transmission quantitative data recording and image processing.

Chris

=====================================================================
DR CHRIS JEFFREE
BIOSEM - BIOLOGICAL SCIENCES EM FACILITY
UNIVERSITY OF EDINBURGH
Daniel Rutherford Building
King's Buildings, Mayfield Road
EDINBURGH, EH9 3JH, Scotland, UK
Tel. #44 131 650 5345
FAX. #44 131 650 6563
Mobile 0410 585 401
email c.jeffree-at-ed.ac.uk
SEM / TEM bookings sem-at-ed.ac.uk
=====================================================================



From daemon Sun Jun 04 10:18:28 2000



From: A.P.Alves de Matos :      apmatos-at-ip.pt
Date: Sat, 3 Jun 2000 22:25:13 +0100
Subject: RE: TEM:beam damage vs voltage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I work with EPON sections of biological specimens. It seems clear to me that
60KV introduces more dammage than 80Kv. The sections are more unstable and
tend to break more easily at the lower voltage.

Dr. A.P. Alves de Matos
Pathology Department
Curry Cabral Hospital
Lisbon




------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Conventional wisdom said that when it came to using TEM for biological
specimens (or beam sensitive specimens) it is always best to use a lower
accelerating voltage. Many lower voltage microscopes (V { 120 kV) were sold
on this assumption.
However is this assumption true? At the present there are many research
establishments, buying TEMs for biological use, who are using 200 to 300 kV
beams. So obviously there has been a shift in the conventional way of
thinking. Being materials based I am not sure what the status quo is in
biological TEM.
I know that the ratio of inelastic to elastic scattering cross sections is
greater than one for the elements Z {12, but how does this change as the
beam energy increases? What are your experiences? This is really just
academic curiosity by the way, but I am sure many of you would appreciate
the question.
********************************************************
Dr Jonathan Barnard

Analytical Materials Physics
The Angstrom Laboratory, Uppsala University
P O Box 534, SE-751 21 Uppsala, Sweden
Phone: +46-(0)18-4716838 Fax: +46-(0)18-500131
Phone: Microscope room +46 18 471 6365
http://www.angstrom.uu.se/analytical/home.html
********************************************************






From daemon Sun Jun 04 10:18:41 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Sat, 03 Jun 2000 19:17:50 -0700
Subject: Re: Resolving power: film vs digital Commercial Post

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 02:21 PM 6/3/00, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

I hope not.


} Digital light microscope cameras are now available that use a highly touted
} CCD detector: Color or monochrome; fast interline transfer, high sensitivity,
} low noise; 6.7 micron square pixel; 2/3 inch 1.3 Million chip; PCI bus frame
} transfer. This detector is ideal to use in light microscopy applications.
} Now precision technology and fast high capacity cheap computers allow this
} detector to be "Micro Stepped" providing digital images of up to 12 million
} pixels to be captured very quickly and providing file sizes of up to 35 MB in
} 24 bit color images. "Inter Pixel Stepping" technology will allow a
} considerably less expensive approach to digital imaging that can now approach
} film resolving capability in a light microscope.
}
} Hats of to Dr. Jeffree's explanation on optical resolution as it relates to
} light microscopy applications. With high pixel density capable digital
} cameras, now lower magnification, low NA (lower resolving power) objectives
} can be used to acquire matching digital resolution images of wide fields of
} view.
}
} Information on Nikon's Instrument Division "Digital Eclipse" family of
} digital cameras for microscopy including the new DXM1200 digital color camera
} will soon be available on our web site www.nikonusa.com or you can go to
} our Dealer Locator at
} http://www.nikonusa.com/corpinfo/dealers/dealerSearch.cfm and ask your
} local Nikon authorized microscope dealer for further information.
}
} Best Regards,
}
} Stan Schwartz
} Manager, BioSciences Dept.
} Nikon Inc Instrument Division
} 1300 Walt Whitman Rd.
} Melville, NY 11747
} 631-547-8500
} 631-547-4033 Fax
} Schwartz-at-nikonincmail.com
} www.nikonusa.com

As a long term past user of Nikon equipment, it is a tale of sorrow.
Most recently, the digital E1 and E2 are dismal failures. Consumers
are sending back 990's in droves (see rec.photo.marketplace.digital).
I really think that Nikon blew it in regards to digicams. How Nikon can
claim to get a 1.3M pixel imager to produce realistic 12M pixel images
is rather absurd....if not offensive. The most recent D1 is a joke. Albeit,
an expensive one.

Nikon blew it in the scanner arena and made huge blunders in high end
pro digicams (E1 & E2). Total junk from my personal experiences. I am
not at all prone to spend a dime on any new Nikon digital things. In fact,
I have
dumped my Nikon so-called pro lenses and bodies for Contax. But this
is another story.

My advice is to be very wary....be very wary of Nikon. I do not use Nikon
cameras anymore and I do not use Nikon microscopes anymore.
But it is your money and your decision. As they say, "Caveat emptor."

gg

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Modern surfers use PC boards. You can too at
http://photoweb.net
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~



From daemon Sun Jun 04 10:19:00 2000



From: Chris Jeffree :      cjeffree-at-srv0.bio.ed.ac.uk
Date: Sun, 4 Jun 2000 09:49:36 +0100
Subject: Re: Resolving power: film vs digital Commercial Post

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Rubbish! With all due respect this is a complete distortion and mis-
use of the point I made. To realise this objective with low NA lenses
you would have to find some way to defeat the laws of physics. No
CCD imager, however clever will make it possible to correct the
shortcomings of cheap, low-performance optics. You clearly
misunderstood the point, which is that microscope lens resoltuion is
not fundamentally dependent on the magnification factor but on the
numerical aperture, which also determines resolution. A x100 NA
1.4 lens resolves no more detail than a x60 NA 1.4, but simply
magnifies the image further. This is "empty magnification", which is
only useful if your image sensor (film or CCD) has limited resolving
power. With a very high resolution film like Technical Pan, you can
take advantage of this fact to capture a wide-field diffraction-limited
image with a x60 1.4 lens. That is not an option with a low NA lens
irrespective of the properties of the sensor, CCD or otherwise.

} Hats of to Dr. Jeffree's explanation on optical resolution as it relates to
} light microscopy applications. With high pixel density capable digital
} cameras, now lower magnification, low NA (lower resolving power) objectives
} can be used to acquire matching digital resolution images of wide fields of
} view.
}
} Information on Nikon's Instrument Division "Digital Eclipse" family of
} digital cameras for microscopy including the new DXM1200 digital color camera
} will soon be available on our web site www.nikonusa.com or you can go to
} our Dealer Locator at
} http://www.nikonusa.com/corpinfo/dealers/dealerSearch.cfm and ask your
} local Nikon authorized microscope dealer for further information.
}
} Best Regards,
}
} Stan Schwartz
} Manager, BioSciences Dept.
} Nikon Inc Instrument Division
} 1300 Walt Whitman Rd.
} Melville, NY 11747
} 631-547-8500
} 631-547-4033 Fax
} Schwartz-at-nikonincmail.com
} www.nikonusa.com
}
}
} Earlier post
} =============================================================
} Geoff
} Analysing this a little further:
} A 25 Mbyte image = 8.3 M pixels to cover 24x36mm or 864mm^2
} this represents 98.2 pixels per mm, or 49 line pairs per mm. Pixel
} size 10.2 um. Resolution about 50 line pairs per mm at best.
}
} A 25M pixels image used to capture a 24x36mm Kodachrome
} slide represents 28,935 pixels per mm^2 or 170 pixels per mm.
} This is equivalent to a maximum resolution of 85 line pairs per mm,
} which may be on the conservative side for Kodachrome. pixel size
} = 5.88um. The image will be approx. 6120x4082 pixels, generating
} a file size of approx 75Mb for a 24-bit (8+8+8bit) RGB image.
}
} To record 120 line pairs per mm, which many top 35mm camera
} lenses can achieve, a minimum of 240 pixels per mm are required,
} each 4.2 um wide. This equates to 8640x5760 pixels for a
} 24x36mm frame = 50M-pixels or 50Mb in 8-bit greyscale, or 150Mb
} in 24-bit RGB.
}
} At 320 line pairs per mm (Technical Pan) the minimum required
} 640 pixels per mm is a pixel size of 1.56 um
}
} Presumably for a light image the diffraction limited resolution is
} approx 1/2 lambda which at 540nm is 0.27um.
} So looking to the future of ultimate-performance CCDs, direct
} recording of a diffraction limited light image projected onto the
} sensor requires at the very least 3703 pixels per image mm or
} 13,717,421 pixels per mm^2 (greyscale 8-bit)
}
} However, if we are doing light microscopy with an NA 1.4 x100
} objective, how much resolving power do we need on CCD or film?
}
} Data is at 0.27um resolution (lambda = 540nm). Let's round this to
} 0.3um. Magnification at 24x36mm film image is x100, so pixels
} must be an absolute maximum of 30um wide to record the
} significant data = 33.3 pixels per mm, equivalent to 800x1200
} pixels to record the whole 35mm negative area. However, most
} CCDs are much smaller than 35 mm frames, typically 1/3 inch. So
} an 800x1200 pixel CCD at 8x12 mm, 1/3 of the linear dimensions
} of a 35mm frame would use 9 pixels to record the smallest image
} details. This is about right from the point of view of resolution, but
} to record the whole 35mm frame we need about 2400x3600 pixels
} on our CCD.
}
} Note also that Technical Pan has (depending on the criterion used
} to assess its performance) up to 10 times the resolving power
} required to record all there is to see in a diffraction-limited LM
} image made with a 100x NA 1.4 lens. So you can comfortably
} afford to use a 60x NA 1.4 lens, thereby getting the same
} resolution with a bigger field of view.
}
} Many years ago, I took a photograph of a street scene using a
} Canon 35mm SLR loaded with Kodak Recordak (I think this was a
} single layer microfilm emulsion). Examined in a light microscope,
} the image clearly, legibly recorded the brand-name of a child's
} push chair. I tried to print this brand name using a DeVere point-
} source enlarger with an image size of 20x30 inches produced with
} a Schneider Componon lens, but was completely unable to
} produce a legible image. The point I am making here is that the
} combination of some high performance films, with high quality
} lenses of the standard produced by the leading camera
} manufacturers can record more detail on the film than you can
} easily get back out by conventional printing. I suspect the same is
} true of EM exposures.
}
} So there is no contest - film beats CCDs for resolution hands
} down. And you can process the image on the cheap. No money
} goes to Intel or Microscoft, Adobe or Epson. But resolution is not
} primarily what we buy CCDs for. We buy them primarily for instant
} image capture in a format suitable for digital storage, digital
} transmission quantitative data recording and image processing.
}
} Chris
}
} =====================================================================
} DR CHRIS JEFFREE
} BIOSEM - BIOLOGICAL SCIENCES EM FACILITY
} UNIVERSITY OF EDINBURGH
} Daniel Rutherford Building
} King's Buildings, Mayfield Road
} EDINBURGH, EH9 3JH, Scotland, UK
} Tel. #44 131 650 5345
} FAX. #44 131 650 6563
} Mobile 0410 585 401
} email c.jeffree-at-ed.ac.uk
} SEM / TEM bookings sem-at-ed.ac.uk
} =====================================================================
}


~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Dr Chris Jeffree
University of Edinburgh
Biological Sciences EM Facility
Daniel Rutherford Building
King's Buildings EDINBURGH EH9 3JH
Tel: +44 (0) 131 650 5345
FAX: +44 (0) 131 650 6563

Inveresk Cottage, 26 Carberry Road,
Inveresk, Musselburgh, Midlothian EH21 8PR, UK
Tel. +44 (0) 131 665 6062 / Mobile 0410 585 401
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~



From daemon Sun Jun 04 10:19:08 2000



From: jim :      jim-at-proscitech.com.au
Date: Sun, 4 Jun 2000 21:20:44 +1000
Subject: RE: Film vs Digital/ switched to EM techn

Contents Retrieved from Microscopy Listserver Archives
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Lets place the exhausted and exhausting Digital topic aside.
Sergey, you do have a challenging project and one that is worth discussing.
Just maybe somebody has an idea that will help you. I think that we discuss in
this forum pixels too much and microscopy too little.

You probably have tried most of my following suggestions, but just incase, here
are a couple of my thoughts:

Dark field contrast is enhanced most when the density between specimen and
background is greatest. So its most effective with no specimen support. You
could try your luck with the superfine mesh grids now available; these thin bar
grids can be purchased down to 2000 mesh and these grids have a 7.5um hole
size. If that is not a fine enough support, then try holey films with a
net-like structure.

Carbon coating will stabilize samples dramatically. A little loss of contrast
is inevitable, but a least carbon on the grid or holey plastic film does not
matter.

Don't see why you want to render the gold nano particles with detail (I assume
some greys). In TEM I would expect gold above about 5nm to be black. In STEM or
FESEM you could get greys easily, but you may not get the resolution required.

Increasing if available to 200 or more the kV, will give more brightness, less
contrast, better resolution (specimen dependent). Most importantly, specimen
damage frequently is less at higher kV, that depends on the specimen again. I
think that damage is less in specimens with greater electron transparency, but
one of our "physical" gurus may explain the whys and wherefores.

Without switching film-types, you can develop the TEM films in things like
Microdol-X, Microphen or Rodinal (hope those developers still exist). You could
also use D-19 more dilute than normal. For most EM purposes all of these would
give too softer negatives, but they just may suit you. Over developing in light
photography yields more contrast - not so in TEM, where more electrons are the
main contrast mechanism. Hence slower film and denser negatives are preferred,
especially by most biologists.

I think that we started out with too much contrast, but a grainy image because
of insufficient electron exposure. Hmmm, I think that we should take a holiday.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Saturday, June 03, 2000 7:44 AM, Sergey Ryazantsev [SMTP:sryazant-at-ucla.edu]
wrote:
}
}
} Dear Jim
}
} I spent about two years trying to make good pictures of my NanoGold labeled
} protein-DNA complexes. Doing this job I find two main problems: the
} sample is unstable under the beam as any biological sample; NanoGold is
} much more bright than protein core in dark-field. I find that it is
} impossible to record equally perfect signals from NanoGold and protein core
} because of short dynamic range for SO-163 film, I believe. I was trying to
} make two pictures with different exposure, but it is tricky: in dark-field
} mode the automatic exposure meter usually does not work and we have to set
} exposure manually, in this case it is difficult to get "right" exposure
} time in the right moment, you know. Again, because of sample's short life
} under the beam, it is impossible to make a couple pictures at the different
} conditions sometime. Keep in mind, please, that to change the film in the
} microscope it takes about 10 seconds. Your idea about increasing
} signal-noise ratio by collecting more electrons is bright but not
} practical. For biological samples (I am talking about non-fixed,
} non-stained samples of proteins, DNA or RNA-protein complexes etc) the
} electron damage is a huge problem. People are trying to solve it in
} different ways. Some using cryo temperature (to stabilize the biological
} structure). I was using freeze-drying (I find that freeze-dried samples
} are more stable under the beam). But in any case we have deal with very
} unstable samples and must to do everything to decrease (not increase as you
} recommended) electron dose. Drift is a second big problem for such
} application: to increase signal-noise ratio we have to use very thin
} support films. Images obtained at such conditions are noisy and in most
} cases we have to use image analysis tools to extract the data. It means
} that we have to digitize our images anyway. In such situation digital
} camera may help. As you, probably, remember I was a person who initiates
} this discussion. I think this discussion was very useful for many of us
} who are not friendly with digital camera's techniques. We understand the
} limitations of the modern digital cameras better now. I would like to say
} thank you everybody who was involved in this discussion. There is some
} conclusions I make for myself from discussion:
}
}
} - Film is still cheap and universal material for recording and
} storage EM images, sorry CCD.
} - CCD TEM camera should not substitute film. Film and camera should
} work all together improving the flexibility of the TEM system. For this
} reason I will chose side-mount camera if will have money for it.
} - For cell-biology (thin sections) where the resolution of the sample
} is about 3 nm CCD camera may do a good job allowing users to make a huge
} number of pictures (cell-biology guys love it), instantly view and
} catalogize them.
} - Sometime the digital camera may help in area of high-resolution
} (relatively high, guys) EM when image will be digitized anyway. The major
} limitation here is small area of view (we need a lot of particles for image
} analysis sometime), but you could make the set of overlapping pictures and
} digitally combine it. I love, also, Mike Bode idea to make a few very
} short exposure pictures and combine it digitally later to reduce noise. The
} relatively big size of CCD's pixels is a real problem too.
} - CCD camera is expensive "toy". I am not sure that the benefits from
} using it will compensate astronomical price, actually the third of the
} electron microscope value ($70000 is it 1/3 of microscope's price on
} current market?). Currently, I would recognize the CCD TEM camera as funny
} "attachment" which may be useful if you rich enough to spend money on it
} (it mean, that you have everything else in your EM lab plus some extra
} $70000 for the fun playing with digital "toy").
} - TEM CCD cameras are under extensive development now. Today's
} camera will be replaced on the new model (read better, faster, what else?)
} next year. Next year's camera will be easily forgotten next after the next
} year and so on... Each new camera will be better that previous one... CCD
} TEM camera it is not a good investment of money, I think.
} - We should keep in mind that many companies charged extra 3-4K$ for
} the installation and training (it is mandatory for Gatan for instance) and
} you, probably, have to buy service contract on it even if you have service
} contract on the microscope (JEOL's service contract on microscope do not
} cover the CCD camera even if you buy it from JEOL).
}
} Best regard, Sergey
}
}
}
}
} } Date: Fri, 02 Jun 2000 21:14:44 +1000
} } From: jim {jim-at-proscitech.com.au}
} } Subject: RE: Film vs Digital
} } To: 'Mike Bode' {mb-at-Soft-Imaging.com} ,
} } "'Microscopy-at-MSA.Microscopy.Com'" {Microscopy-at-sparc5.microscopy.com}
} } Cc: "'jim-at-proscitech.com.au'" {jim-at-proscitech.com.au}
} } Reply-to: "jim-at-proscitech.com.au" {jim-at-proscitech.com.au}
} } Organization: ProSciTech
} } X-Mailer: Microsoft Internet E-mail/MAPI - 8.0.0.4211
} }
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } The world is full of possible solutions, but are they practical.?
} } To produce high-resolution, dark-field or any others TEM images that require
} } more electrons to form a clear image, Mike Bode would use multiple digital
} } exposures. The exposures could be layered and combined into one superior
} } image.
} } This image would be made up of more pixel and is formed by more electrons
} } and
} } so would be noise-free and hence could be further enlarged then otherwise
} } possible. Perhaps.
} } Beam blanking would largely save the specimen from beam damage and drift
} } could
} } be compensated for by matching up the digitals. Great.
} } How much time is required between exposures to transfer a minimum 10mb image
} } per exposure? What would be the total time from focusing to the last
} } exposure?
} } What about Z-drift in the interim requiring objective changes and what about
} } the total cost of this additional get-up. The mind boggles at a through
} } focus
} } series.
} } When pushing the limits a piece of film seems more effective, cheaper and fa
} } ster.
} } Again, I don't doubt that there is now a large place for digital in TEM, but
} } its no panacea.
} } Cheers
} } Jim Darley
} } ProSciTech Microscopy PLUS
} } PO Box 111, Thuringowa QLD 4817 Australia
} } Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
} } Great microscopy catalogue, 500 Links, MSDS, User Notes
} } www.proscitech.com
} }
} } On Friday, June 02, 2000 1:24 AM, Mike Bode [SMTP:mb-at-soft-imaging.com]
wrote:
} }
} } }
} } } No, it's not really a problem. It's been done with low density
} } } microscopy all the time. Granted, there are some technical aspects to be
} } } overcome, but (and I can only speak for ourselves) we have done that on
} } } a number of microscopes. You are of course correct, that 10 images at
} } } 0.8 seconds take longer than 8 seconds as the image has to be
} } } transferred, etc. BUT: that's what beam blankers are for. It is pretty
} } } straightforward to take an image at 0.8 seconds, then blank the beam
} } } very quickly before taking the next image. That way you get pretty close
} } } to the 8 sec total exposure. If there is no beam blanker on the
} } } microscope, in most cases it can be added.
} } }
} } } I am not sure what you mean by "too sensitive". The cameras are usually
} } } constructed so that 1 electron from the beam creates between a few tenth
} } } to a few counts (these are all statistical data, of course). The well
} } } width divided by this sensitivity then determines, how many primary
} } } electrons are needed to fully expose one pixel. For example, if the well
} } } width is 50,000 electrons and the sensitivity is 1 count/electron, one
} } } needs 50,000 primary electrons to fill the well. This translates into
} } } roughly a 0.4% statistical error.
} } }
} } } } From a practical standpoint: You can take images with most cameras when
} } } the exposure meter on the microscope reads a couple of seconds without
} } } overexposing the camera. On the other hand, you can reduce the intensity
} } } of the beam until you see single electron events.
} } }
} } } The one area where CCD cameras may be too sensitive is diffraction. The
} } } normally huge intensity in the transmitted beam often leads to
} } } saturation. In CCDs this can lead to blooming (the intensity spills over
} } } into neighboring pixels). This can be taken care of with special chips
} } } that have anti-blooming features, but this usually has some other
} } } drawbacks. Again, this can also be overcome somewhat with multiple
} } } exposures. Film behaves more civilized here, as it simply stops
} } } responding to the electrons, but this makes film more or less useless
} } } for quantitative measurements of diffraction patterns. I have done
} } } diffraction with CCDs many times and though it does require some
} } } tweaking, one can get very good results from them.
} } }
} } } Michael Bode, Ph.D.
} } } Soft Imaging System Corp.
} } } 1675 Carr St., #105N
} } } Lakewood, CO 80215
} } } ===================================
} } } phone: (888) FIND SIS
} } } (303) 234-9270
} } } fax: (303) 234-9271
} } } email: mailto:info-at-soft-imaging.com
} } } web: http://www.soft-imaging.com
} } } ===================================
} } }
} } }
} } }
} } } -----Original Message-----
} } } From: jim [mailto:jim-at-proscitech.com.au]
} } } Sent: Wednesday, May 31, 2000 9:37 PM
} } } To: 'Mike Bode'; 'Microscopy-at-MSA.Microscopy.Com'
} } } Subject: RE: Film vs Digital
} } }
} } }
} } } ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } -----------------------------------------------------------------------.
} } }
} } }
} } } Yes, it could be done "in theory". Somebody would need to figure out the
} } }
} } } software and perhaps modify the hardware. Then we would find that the
} } } total
} } } exposure of the specimen to the electron beam maybe a muliple of the
} } } film's
} } } exposure. Afterall, an 8 sec film exposure would not amount in digital
} } } to
} } } 10x0.8, but we would require considerable time in between exposures.
} } } Since the
} } } problems in the discussed circumstances are specimen movement and beam
} } } damage,
} } } it seems that taking multiple exposures is a poor option.
} } }
} } } Digital cameras are for some situation too sensitive to electron
} } } exposure.
} } } Cutting back on electrons is no option since its the electrons that form
} } } the
} } } image in the first instance.
} } } Much easier in light microscopy . . . insert a neutral density filter.
} } } Cheers
} } } Jim Darley
} } } ProSciTech Microscopy PLUS
} } } PO Box 111, Thuringowa QLD 4817 Australia
} } } Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
} } } Great microscopy catalogue, 500 Links, MSDS, User Notes
} } } www.proscitech.com
} } }
}
} _____________________________________
}
} Sergey Ryazantsev Ph. D.
} Electron Microscopy
} UCLA School of Medicine
} Department of Biological Chemistry
} Box 951737
} Los Angeles, CA 90095-1737
}
} Phone: (310) 825-1144
} Pager: (310) 845-0248
} FAX (departmental): (310) 206-5272
} mailto:sryazant-at-ucla.edu
} http://www.bol.ucla.edu/~sryazant
}
}



From daemon Sun Jun 04 10:19:08 2000



From: jim :      jim-at-proscitech.com.au
Date: Sun, 4 Jun 2000 18:33:42 +1000
Subject: RE: Film vs Digital

Contents Retrieved from Microscopy Listserver Archives
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I have two replies and Mike my well have four replies to these.
1. There has been a parallel discussion concerning resolution of film versus
digital images. Clearly in raw power digital cannot compete since film has
multi gigabyte capacity. I added to that thread that what matters is: does
digital have enough power and that frequently it would. Mike reinforced and
strengthened that argument, finishing with the note that he is glad for film
when looking at prints by Ansel Adams.
(Ansel Adams until about 30 years ago carted for decades large format cameras
through US National Parks, especially Yosemite, producing fantastic landscape
photographs and books)
Adams' limited edition prints were contacts of 5x7 and 8x10" inch sheet film,
probably rated at 400 ISO. The line resolution of such prints much exceeds our
eyes' resolution, but still results in superior gradation and detail. TEM film,
even when much enlarged has such details too.
Why Mike, should we accept 2.5 Mb? That is a splendid file size for SEM, and
because of the limited enlargability of light microscopy (concrete ceiling due
to wavelengths) it's reasonable, but minimal for light microscopy. TEM can do
and deserves better.

Incidentally, from the outset I cited increased "enlargability" to obtain high
resolution TEM, as one of films major advantages, since greater depths of field
at moderate powers makes high powers through photo enlarging a desirable
technique. The small additional magnification yielded by placing a digital
camera lower in the column does not compensate. So greater "enlargability" of
digitals would be desirable, but is limited by pixel size.

2 The thread was initiated by Sergey. He had problems visualising certain
specimens in dark field. The use of his "beaut" digital TEM camera made things
worse. I pointed out that the shorter exposure reduced the number of electrons
forming the image, hence more noise. I believe that a good part of Mike's case
will be settled in his favour when we hear "Eureka" from Sergey's lab. I don't
doubt that much more can be done with digital now and that further improvements
are on the way. Mike's "solution" may well be possible, but I don't believe its
a snap; in any case: Show Sergey!
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Saturday, June 03, 2000 2:58 AM, Mike Bode [SMTP:mb-at-Soft-Imaging.com] wrote:
}
} Well, let's see:
}
} Jim wrote:
}
} Mike Bode would use multiple digital
} exposures. The exposures could be layered and combined into one superior
} image.
} This image would be made up of more pixel and is formed by more
} electrons and
} so would be noise-free and hence could be further enlarged then
} otherwise
} possible. Perhaps.
}
} No, I did not talk about further enlargements. All I wanted to say is,
} that a more noise-free image can be achieved by adding multiple images,
} and that this also to some extent helps with drift of the sample during
} acquisition.
}
} Jim wrote:
}
} How much time is required between exposures to transfer a minimum 10mb
} image
} per exposure?
}
} How did you arrive at 10 MB? A 1280x1024 image with 16 bit pixel
} information is about 2.5 MB (uncompressed). We acquire about 10 of those
} per second and transfer them across the PC bus to the display. Putting
} them on them into Memory might add a few tenth of a second. Writing to
} HD can be done after all images are acquired.
}
} Jim wrote:
}
} What would be the total time from focusing to the last exposure?
} What about Z-drift in the interim requiring objective changes
}
} Why would we have to worry about that, if we don't have to worry about
} that when taking the image on film? In fact, we could take care of this
} by looking at the image between exposures and correct for z-drift.
} However, as you said, that would add to the overall time and exposure. I
} was comparing a normal dark field image taken on film at 8 seconds with
} acquiring the same image on a "too sensitive" CCD camera by adding up 10
} consecutive .8 second images. Why would the sample drift (in x, y or z)
} substantially more in 8+delta seconds than in 8?
}
} Jim wrote:
}
} what about the total cost of this additional get-up
}
} That of course depends on the microscope and there is no general answer.
} For example on a LEO 912 I believe the blanker is standard. The
} additional cost to use an acquisition scheme like this with our software
} is $0 plus perhaps a bit of time to write a small macro. On other
} microscopes one might have to add a beam blanker and perhaps a control
} mechanism for the beam blanker. But I would guess, that this cost is not
} very high. All modern microscopes are computer controller anyway, so it
} is most likely just a control command that needs to be sent to the
} microscope over a serial port if the beam blanker is installed. Piece of
} cake.
}
} Jim wrote:
}
} The mind boggles at a through focus series.
}
} You're right here. But I don't think we were talking about through-focus
} series. Incidentally, we do through-focus series on light microscopes
} and reconstruction routinely. Takes a few images at different focus (or
} for a light microscope: stage) settings. The rest is done off-line.
} Takes maybe a couple of minutes for about 20 images of about 1kx1k. I
} agree that TEM is different here and much more complicated due to the
} complicated Contrast Transfer Function. However, this could in principle
} be sorted out.
}
} Jim wrote:
}
} Again, I don't doubt that there is now a large place for digital in TEM,
} but
} its no panacea.
}
} I also agree with you on that one. But using the additional computer
} possibilities of digital imaging might take you further than expected.
}
} Michael
}
}
} Michael Bode, Ph.D.
} Soft Imaging System Corp.
} 1675 Carr St., #105N
} Lakewood, CO 80215
} ===================================
} phone: (888) FIND SIS
} (303) 234-9270
} fax: (303) 234-9271
} email: mailto:info-at-soft-imaging.com
} web: http://www.soft-imaging.com
} ===================================
}
}
}
} -----Original Message-----
} } From: jim [mailto:jim-at-proscitech.com.au]
} Sent: Friday, June 02, 2000 5:15 AM
} To: 'Mike Bode'; 'Microscopy-at-MSA.Microscopy.Com'
} Cc: 'jim-at-proscitech.com.au'
} Subject: RE: Film vs Digital
}
}
} The world is full of possible solutions, but are they practical.?
} To produce high-resolution, dark-field or any others TEM images that
} require
} more electrons to form a clear image, Mike Bode would use multiple
} digital
} exposures. The exposures could be layered and combined into one superior
} image.
} This image would be made up of more pixel and is formed by more
} electrons and
} so would be noise-free and hence could be further enlarged then
} otherwise
} possible. Perhaps.
} Beam blanking would largely save the specimen from beam damage and drift
} could
} be compensated for by matching up the digitals. Great.
} How much time is required between exposures to transfer a minimum 10mb
} image
} per exposure? What would be the total time from focusing to the last
} exposure?
} What about Z-drift in the interim requiring objective changes and what
} about
} the total cost of this additional get-up. The mind boggles at a through
} focus
} series.
} When pushing the limits a piece of film seems more effective, cheaper
} and fa
} ster.
} Again, I don't doubt that there is now a large place for digital in TEM,
} but
} its no panacea.
} Cheers
} Jim Darley
} ProSciTech Microscopy PLUS
} PO Box 111, Thuringowa QLD 4817 Australia
} Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
} Great microscopy catalogue, 500 Links, MSDS, User Notes
} www.proscitech.com
}
} On Friday, June 02, 2000 1:24 AM, Mike Bode [SMTP:mb-at-soft-imaging.com]
} wrote:
} }
} } No, it's not really a problem. It's been done with low density
} } microscopy all the time. Granted, there are some technical aspects to
} be
} } overcome, but (and I can only speak for ourselves) we have done that
} on
} } a number of microscopes. You are of course correct, that 10 images at
} } 0.8 seconds take longer than 8 seconds as the image has to be
} } transferred, etc. BUT: that's what beam blankers are for. It is pretty
} } straightforward to take an image at 0.8 seconds, then blank the beam
} } very quickly before taking the next image. That way you get pretty
} close
} } to the 8 sec total exposure. If there is no beam blanker on the
} } microscope, in most cases it can be added.
} }
} } I am not sure what you mean by "too sensitive". The cameras are
} usually
} } constructed so that 1 electron from the beam creates between a few
} tenth
} } to a few counts (these are all statistical data, of course). The well
} } width divided by this sensitivity then determines, how many primary
} } electrons are needed to fully expose one pixel. For example, if the
} well
} } width is 50,000 electrons and the sensitivity is 1 count/electron, one
} } needs 50,000 primary electrons to fill the well. This translates into
} } roughly a 0.4% statistical error.
} }
} } } From a practical standpoint: You can take images with most cameras
} when
} } the exposure meter on the microscope reads a couple of seconds without
} } overexposing the camera. On the other hand, you can reduce the
} intensity
} } of the beam until you see single electron events.
} }
} } The one area where CCD cameras may be too sensitive is diffraction.
} The
} } normally huge intensity in the transmitted beam often leads to
} } saturation. In CCDs this can lead to blooming (the intensity spills
} over
} } into neighboring pixels). This can be taken care of with special chips
} } that have anti-blooming features, but this usually has some other
} } drawbacks. Again, this can also be overcome somewhat with multiple
} } exposures. Film behaves more civilized here, as it simply stops
} } responding to the electrons, but this makes film more or less useless
} } for quantitative measurements of diffraction patterns. I have done
} } diffraction with CCDs many times and though it does require some
} } tweaking, one can get very good results from them.
} }
} } Michael Bode, Ph.D.
} } Soft Imaging System Corp.
} } 1675 Carr St., #105N
} } Lakewood, CO 80215
} } ===================================
} } phone: (888) FIND SIS
} } (303) 234-9270
} } fax: (303) 234-9271
} } email: mailto:info-at-soft-imaging.com
} } web: http://www.soft-imaging.com
} } ===================================
} }
} }
} }
} } -----Original Message-----
} } From: jim [mailto:jim-at-proscitech.com.au]
} } Sent: Wednesday, May 31, 2000 9:37 PM
} } To: 'Mike Bode'; 'Microscopy-at-MSA.Microscopy.Com'
} } Subject: RE: Film vs Digital
} }
} }
} }
} ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} } To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} } On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} }
} -----------------------------------------------------------------------.
} }
} }
} } Yes, it could be done "in theory". Somebody would need to figure out
} the
} }
} } software and perhaps modify the hardware. Then we would find that the
} } total
} } exposure of the specimen to the electron beam maybe a muliple of the
} } film's
} } exposure. Afterall, an 8 sec film exposure would not amount in digital
} } to
} } 10x0.8, but we would require considerable time in between exposures.
} } Since the
} } problems in the discussed circumstances are specimen movement and beam
} } damage,
} } it seems that taking multiple exposures is a poor option.
} }
} } Digital cameras are for some situation too sensitive to electron
} } exposure.
} } Cutting back on electrons is no option since its the electrons that
} form
} } the
} } image in the first instance.
} } Much easier in light microscopy . . . insert a neutral density
} filter.
} } Cheers
} } Jim Darley
} } ProSciTech Microscopy PLUS
} } PO Box 111, Thuringowa QLD 4817 Australia
} } Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
} } Great microscopy catalogue, 500 Links, MSDS, User Notes
} } www.proscitech.com
} }



From daemon Sun Jun 04 10:19:09 2000



From: Lawrence Kordon :      nikon-at-jagunet.com
Date: Sun, 04 Jun 2000 09:53:36 -0400
Subject: Re: Resolving power: film vs digital Commercial Post

Contents Retrieved from Microscopy Listserver Archives
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****This is a commercial response from a Vendor****

Gary and List,

I am sorry you have had a bad experience with Nikon products. However, you are
entitled to your "opinion" and therefore; I am entitled to a response. First, the
new Digital Eclipse Camera capable of 12M pixels using a stepper mode is exactly
the same technology that the new Zeiss Axiocam and an Olympus model uses which has
been very well received by the microscope community.

Next, the successor to E1, E2 (technically, made by Fuji) is the current Nikon D1
(Digital SLR) was NOT intended for microscopes. It CAN go on one, but is limited
to Brightfield applications and has no NTSC (video) out for focusing. However, it
is by far the standard in Digital Cameras! It has won every Journal Award in it's
field and is the definitive choice for Professional Photo-Journalists, including
the massive market share Nikon has and the majority of Pulitzer Prize winners. As
for Film Scanner products, the Nikon Coolscan line is still the standard in the
industry. Especially, high end units with Auto Feeders. All Nikon digital products
are not perfect. However, these are technical products that are evolving 6 months
at a time and are truly in their infantile state from where they will be in just a
few years from now! I would prefer to address your specific problems, but you gave
none and just called it "junk". With that mentality, I feel my Pentium 266
computer is "junk", but I do not blame the manufacturer because I own an older
model.

As for the Nikon Coolpix line (990), pardon my sarcasm, but where are "all the
returns" because we need them to fulfill the 38,000 unit Backorders! This camera
is so wildly successful EVERY Dealer (Photo and Microscope) is begging for them.
Check out Ebay for example; the cameras are selling for more than List Price.
Sound to me like a success if you understand the basics of Economics and Supply
and Demand. Is it the perfect microscope camera? No, I don't even think so...but
it is the ONLY high resolution (3.34M) digital camera, with live video out, goes
on any brand or model microscope for under $1000. Oh, did I forget to mention it
also won almost every Magazine Award in its class (not all, it actually received a
tie with the Olympus 3030 in one, which is a very nice camera, but does not mount
a microscope).

Finally, I have been on this List for many years and have respected the use and
rules of this forum. We Vendors for the most part are respectful of the opinions
of our users. However, making a "blanket statement" like "stay away from Nikon",
does not serve the public well; NOR YOURSELF! If you or any customer has specific
problems; we want to know about it; as does any reputable manufacturer for future
product improvement. The fact is Nikon has the largest market share in microscopes
and professional camera equipment and is climbing very quickly on the digital
camera list too. So, in short you are entitled to your opinion, but the rest of
the world by far does NOT agree with it............

I personally apologize to any members this correspondence offends. Believe me, my
preference is to serve the list and answer technical microscopy questions as I
have for many years, but this unfair and libel attack required a response.

Regretfully,

Lawrence Kordon
Nikon, Inc.
Senior Bioscience Specialist
nikon-at-jagunet.com

"Dr. Gary Gaugler" wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} At 02:21 PM 6/3/00, you wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Hello all,
} }
} } Please be aware that this is a commercial post that may be of interest to
} } some of the list members.
}
} I hope not.
}
} } Digital light microscope cameras are now available that use a highly touted
} } CCD detector: Color or monochrome; fast interline transfer, high sensitivity,
} } low noise; 6.7 micron square pixel; 2/3 inch 1.3 Million chip; PCI bus frame
} } transfer. This detector is ideal to use in light microscopy applications.
} } Now precision technology and fast high capacity cheap computers allow this
} } detector to be "Micro Stepped" providing digital images of up to 12 million
} } pixels to be captured very quickly and providing file sizes of up to 35 MB in
} } 24 bit color images. "Inter Pixel Stepping" technology will allow a
} } considerably less expensive approach to digital imaging that can now approach
} } film resolving capability in a light microscope.
} }
} } Hats of to Dr. Jeffree's explanation on optical resolution as it relates to
} } light microscopy applications. With high pixel density capable digital
} } cameras, now lower magnification, low NA (lower resolving power) objectives
} } can be used to acquire matching digital resolution images of wide fields of
} } view.
} }
} } Information on Nikon's Instrument Division "Digital Eclipse" family of
} } digital cameras for microscopy including the new DXM1200 digital color camera
} } will soon be available on our web site www.nikonusa.com or you can go to
} } our Dealer Locator at
} } http://www.nikonusa.com/corpinfo/dealers/dealerSearch.cfm and ask your
} } local Nikon authorized microscope dealer for further information.
} }
} } Best Regards,
} }
} } Stan Schwartz
} } Manager, BioSciences Dept.
} } Nikon Inc Instrument Division
} } 1300 Walt Whitman Rd.
} } Melville, NY 11747
} } 631-547-8500
} } 631-547-4033 Fax
} } Schwartz-at-nikonincmail.com
} } www.nikonusa.com
}
} As a long term past user of Nikon equipment, it is a tale of sorrow.
} Most recently, the digital E1 and E2 are dismal failures. Consumers
} are sending back 990's in droves (see rec.photo.marketplace.digital).
} I really think that Nikon blew it in regards to digicams. How Nikon can
} claim to get a 1.3M pixel imager to produce realistic 12M pixel images
} is rather absurd....if not offensive. The most recent D1 is a joke. Albeit,
} an expensive one.
}
} Nikon blew it in the scanner arena and made huge blunders in high end
} pro digicams (E1 & E2). Total junk from my personal experiences. I am
} not at all prone to spend a dime on any new Nikon digital things. In fact,
} I have
} dumped my Nikon so-called pro lenses and bodies for Contax. But this
} is another story.
}
} My advice is to be very wary....be very wary of Nikon. I do not use Nikon
} cameras anymore and I do not use Nikon microscopes anymore.
} But it is your money and your decision. As they say, "Caveat emptor."
}
} gg
}
} ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
} Modern surfers use PC boards. You can too at
} http://photoweb.net
} ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~



From daemon Sun Jun 04 12:19:11 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Sun, 4 Jun 2000 12:26:35 -0500
Subject: Administrivia: Resolving power: film vs digital Commercial Post

Contents Retrieved from Microscopy Listserver Archives
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Colleagues.....

Stop this "tangental thread" on Resolving Power now, before it
gets out of hand.
User/Vendor problems should be handled in private not on the list.


Nestor
Your Friendly Neighborhood SysOp.




From daemon Sun Jun 04 21:46:07 2000



From: Caroline Schooley :      schooley-at-mcn.org
Date: Sun, 4 Jun 2000 16:29:41 -0700
Subject: Project MICRO

Contents Retrieved from Microscopy Listserver Archives
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It's been some time since I posted news about Project MICRO, MSA's middle
school educational outreach program. I'm happy to report that Nestor,
wearing his Webmaster hat (yes, he owns more than one), has just posted a
substantial revision of the MICRO website (URL below). You'll find new
information on several pages and a LOT of new entries in the bibliography.
Don't miss the "Cyclops" videos and the new CD-ROMs; the website hotlinks
are much expanded also.

Caroline

Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html




From daemon Sun Jun 04 22:06:23 2000



From: John E. Toth :      jetoth-at-mail.olypen.com
Date: Sun, 4 Jun 2000 21:53:51 -0500
Subject: Microsope design

Contents Retrieved from Microscopy Listserver Archives
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Sirs,

I am looking for books on the subject of optical microscope objective
lens design.
Any suggestions would be appreciated.

John Toth

jetoth-at-olypen.com
or
sciret-at-olypen.com




From daemon Sun Jun 04 22:06:23 2000



From: Toby Knight :      tknight-at-waite.adelaide.edu.au
Date: Mon, 5 Jun 2000 12:29:02 +0930 (CST)
Subject: schiffs staining epoxy sections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I want to stain Procure-Araldite embedded material using the periodic-acid
schiff procedure. One method I have is to hydrolyze in 1N HCl at
60 degrees C for 10 mins, water wash, stain in schiffs, metabisulphate
rinses, counterstain, water wash (O'Brien and McCully, 1981). Does anyone
use a method routinely which varies from this??

thanks in advance, Toby Knight.


--------------------------------------------------------
Toby Knight
PhD student

Department of Horticulture, Viticulture and Oenology
The University of Adelaide
Plant Research Centre
Waite Campus, PMB 1
Glen Osmond, SA 5064

Tel: +61 8 8303 7224 or 8303 6668
HVO: +61 8 8303 7242
Fax: +61 8 8303 7116
Email: tknight-at-waite.adelaide.edu.au
--------------------------------------------------------





From daemon Sun Jun 04 22:46:41 2000



From: MGMANDERS-at-aol.com
Date: Sun, 4 Jun 2000 22:41:14 -0500
Subject: NEED HELP WITH EDS ON A JEOL 100S

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A fellow colleague has bought a JEOL 100s and wants and needs EDS X-ray
Analysis. He's mounted his horizintal EDS detector , but can not get sample
X-rays from the speciman. Contacting JEOL he found the problem to be a tilt
problem. The 10 degree tilt from the normal SEG only tilts 10 degrees and a
30 to 60 degree tilt is necessary. At one time I was told that special
sample holders and or double gap pole pieces were availabe. He wishs to find
any one with a 100s for parts needed or information which would allow X-ray
analysis. Please reply to mgmanders-at-aol.com or the list server.

Mike Manders




From daemon Sun Jun 04 23:59:57 2000



From: Gordon Couger :      gcouger-at-couger.com
Date: Sun, 4 Jun 2000 23:46:40 -0500
Subject: Desktop incubator

Contents Retrieved from Microscopy Listserver Archives
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I reacall some one looking for a desktop incubator. I came
a across one on ebay.
http://cgi.ebay.com/aw-cgi/eBayISAPI.dll?ViewItem&item=344913999
It is a little pricy by ebay standards. You can find a bunch of incubators
by going to www.ebay.com and search for incubators you can do the
same a http://www.labx.com

I don't have any connection with anyone involved in this.

Gordon

Gordon Couger gcouger-at-couger.com

Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00




From daemon Mon Jun 05 08:21:42 2000



From: John Nailon :      mmjnailo-at-dingo.cc.uq.edu.au
Date: Sat, 03 Jun 2000 16:35:28 +1000
Subject: Re: schiffs staining epoxy sections

Contents Retrieved from Microscopy Listserver Archives
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Toby,
I used this technique years ago when I worked for Terry O'Brien at
Monash Uni. Depending on the type of tissue you may want to do an
aldehyde blockade before you begin the staining procedure. This
technique is also in O'Brien and McCully. The blockade removes any
aldehyde grouping that will react with the Schiffs reagent. You then
generate and stain aldehyde groups during the procedure.
Regards
JVN

Toby Knight wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} I want to stain Procure-Araldite embedded material using the periodic-acid
} schiff procedure. One method I have is to hydrolyze in 1N HCl at
} 60 degrees C for 10 mins, water wash, stain in schiffs, metabisulphate
} rinses, counterstain, water wash (O'Brien and McCully, 1981). Does anyone
} use a method routinely which varies from this??
}
} thanks in advance, Toby Knight.
}
} --------------------------------------------------------
} Toby Knight
} PhD student
}
} Department of Horticulture, Viticulture and Oenology
} The University of Adelaide
} Plant Research Centre
} Waite Campus, PMB 1
} Glen Osmond, SA 5064
}
} Tel: +61 8 8303 7224 or 8303 6668
} HVO: +61 8 8303 7242
} Fax: +61 8 8303 7116
} Email: tknight-at-waite.adelaide.edu.au
} --------------------------------------------------------

--
****************************************************
John V Nailon
Operations Manager
Centre for Microscopy and Microanalysis
The University of Queensland
St. Lucia Queensland 4072
Phone: +61-7-3365-4214
Fax: +61-7-3365-4422
WWW: http://www.uq.edu.au/nanoworld/allstaff.html#Nailon
****************************************************



From daemon Mon Jun 05 08:21:57 2000



From: Michelle.Taurino-at-aventis.com
Date: Mon, 5 Jun 2000 06:54:20 -0500
Subject: schiffs staining epoxy sections

Contents Retrieved from Microscopy Listserver Archives
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Toby-
I've worked with the PAS stain with methyl methacrylate embedded tissue cut
at 2 microns.
I used a kit from Polyscientific (NY, USA) which supplied me with all of the
necessary reagents (minus the ethanol and xylene). The protocol that was
supplied with the kit is for paraffin embedded material but I made
modifications to the protocol to accommodate methyl methacrylate.
If you are interested in the modified protocol, please contact me.
I should also mention that I automated this stain to save time and to
maximize quality.

Good luck!
Michelle Taurino
Aventis Pharmaceuticals
Bioimaging and Molecular Histology
Tel-908-231-3357
Fax-908-231-3962
e-mail: Michelle.Taurino-at-aventis.com


-----Original Message-----
} From: Toby Knight [mailto:tknight-at-waite.adelaide.edu.au]
Sent: Sunday, June 04, 2000 10:59 PM
To: Histonet; Microscopy list


I want to stain Procure-Araldite embedded material using the periodic-acid
schiff procedure. One method I have is to hydrolyze in 1N HCl at
60 degrees C for 10 mins, water wash, stain in schiffs, metabisulphate
rinses, counterstain, water wash (O'Brien and McCully, 1981). Does anyone
use a method routinely which varies from this??

thanks in advance, Toby Knight.


--------------------------------------------------------
Toby Knight
PhD student

Department of Horticulture, Viticulture and Oenology
The University of Adelaide
Plant Research Centre
Waite Campus, PMB 1
Glen Osmond, SA 5064

Tel: +61 8 8303 7224 or 8303 6668
HVO: +61 8 8303 7242
Fax: +61 8 8303 7116
Email: tknight-at-waite.adelaide.edu.au
--------------------------------------------------------




From daemon Mon Jun 05 08:21:58 2000



From: Dr Malcolm Roberts :      malc-at-rock.ru.ac.za
Date: Mon, 05 Jun 2000 14:01:28 +0200
Subject: BSE-Thanks

Contents Retrieved from Microscopy Listserver Archives
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Just like to thank all those who responded to my squeal for help on BS
detector resolution. Would like to add that the South African microscopy
community is looking into establishing QA procedures and some of the
suggestions maybe very helpful in this regard.
Thanks,
Malc.

--
Dr MP Roberts Phone: [+27](0)46 603 8313
Dept of Geology Fax: [+27](0)46 622 9715
Rhodes University Cell: 083 4060 262 (usually off)
6140 Grahamstown e-mail: malc-at-rock.ru.ac.za
SOUTH AFRICA




From daemon Mon Jun 05 08:42:06 2000



From: De Pauw Bart :      bart.depauw-at-rug.ac.be
Date: Mon, 05 Jun 2000 15:21:00 +0200
Subject: SEM - Mycoplasms

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Hello Listers,

I've been trying to look at Mycoplasms with SEM. Untill now, the results
are not what we hoped for. We've used polycarbonate filters to prepare the
Mycoplasms. Another way was preparing the Mycoplasms on agar. We have done
this already for enterococcus and we know that this technique works well.
But with the Mycoplasms we see almost nothing. It's like the Mycoplasms are
IN the Agar.
Has anyone have experience with MYCOPLASMS and SEM ???


De Pauw Bart
Ghent University
Faculty of Veterinary Medicine
Morphology
Salisburylaan 133
9820 Merelbeke
Belgium
Phone : 0032(0)9 264.77.19
Fax : 0032(0)9 264.77.90



From daemon Mon Jun 05 10:02:23 2000



From: Chris Jeffree :      cjeffree-at-srv0.bio.ed.ac.uk
Date: Mon, 5 Jun 2000 16:02:06 +0000
Subject: resolution of Technical Pan

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Toby
The procedure you describe is not in fact Periodic Acid-Schiff but
the Feulgen reaction, for visualization of DNA in nuclei and
mitochondria.

The PAS reaction uses periodic acid or sodium meta-periodate
(typically 1% solution, ~10min) to oxidise the vicinal diols of some
polysaccharides which are then stained with pararosaniline Schiff's
reagent. It is not usually necessary to use metabisulphite in the
wash water. The detailed procedure is also described in O'Brien &
McCully 1981.

PAS (and Feulgen) may be viewed in a brightfield microscope or by
fluorescence microscopy with a "rhodamine" filter set. An
alternative fluorescent dye which works well in a
fluorescence pseudo Schiff procedure is Lucifer Yellow CH, which
needs blue excitation (FITC filter set).

Chris


Date sent: Mon, 5 Jun 2000 12:29:02 +0930 (CST)
} From: Toby Knight {tknight-at-waite.adelaide.edu.au}
Send reply to: Toby Knight {tknight-at-waite.adelaide.edu.au}


I just came across the following pearl in a 1982 Kodak publication,
number PDS 61 "Selecting film from Kodak for photomicrography".

"The (Technical Pan) 2415 film has very low granularity and is
capable of very great enlargement. In some cases the resolving
power may be beyond that of the microscope image"

How do they know!
Answers on a postcard please ....

Chris
=====================================================================
DR CHRIS JEFFREE
BIOSEM - BIOLOGICAL SCIENCES EM FACILITY
UNIVERSITY OF EDINBURGH
Daniel Rutherford Building
King's Buildings, Mayfield Road
EDINBURGH, EH9 3JH, Scotland, UK
Tel. #44 131 650 5345
FAX. #44 131 650 6563
Mobile 0410 585 401
email c.jeffree-at-ed.ac.uk
SEM / TEM bookings sem-at-ed.ac.uk
=====================================================================



From daemon Mon Jun 05 10:12:29 2000



From: Charlesworth, Jon :      charlesworth.jon-at-mayo.edu
Date: Mon, 5 Jun 2000 10:06:40 -0500
Subject: Technician Position

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Electron Microscopy Core Facility at Mayo Clinic

The Electron Microscopy Core Facility at Mayo Clinic in
Rochester, MN has an opening for a Biomedical EM technologist to support
both clinical and research projects. The laboratory offers expertise to
collaborative projects that involve transmission and scanning electron
microscopy. The laboratory is well equipped and has a history of excellent
productivity and adequate funding.

The successful candidate for this position will possess at
least a bachelor's degree in Biology with experience in histology and/or
electron microscopy. Additional courses or experience in Immunology, Cell
Biology, and Digital Imaging is desirable. Operating knowledge of
transmission and scanning electron microscopes is preferred. The applicant
must have excellent communicative skills and the ability to work well with a
variety of personalities.

The EM Technologist interacts with all laboratory users in
order to accomplish specific research and clinical goals with respect to
electron microscopy procedures. Duties include: All aspects of specimen
preparation for a variety of biomedical samples for TEM and SEM, operation
of TEM and SEM, darkroom developing and printing, digital image capture, and
reporting. The technologist will also perform advanced research procedures
including immunoelectron microscopy, x-ray microanalysis, and microwave
processing.

Mayo offers a competitive salary and benefits package. If
interested, please submit a cover letter and resume referencing job posting
#00-0002365 to:

Jill Kelly
Mayo Medical Center
Human Resources-OE 1
Rochester, MN 55905
Fax: 507-284-1445
Email: kelly.jill-at-mayo.edu



Jon Charlesworth
Coordinator
Electron Microscopy Core Facility
1426 Gugg
X4-3148



From daemon Mon Jun 05 10:32:17 2000



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Mon, 5 Jun 2000 09:10:09 -0600
Subject: RE: Film vs Digital

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Jim wrote:

'I have two replies and Mike my well have four replies to these.'

I'll try. But I agree with you that we should let this thread die. jim,
it seems you're a bit angy at me. If I unintentionally stepped on your
toe I apologize. I did not expect that we would agree 100%, as you are
selling film and I am involved in digital systems. I just hope that some
other readers found some of this useful. I don't consider myself a
"digital fanatic". This film vs. digital issue has many more facets,
some of which we did not even touch, and which can be just as
entertaining.

Jim wrote:

'Mike reinforced and strengthened that argument, finishing with the note
that he is glad for film when looking at prints by Ansel Adams.'

Just for the record: I usually do not take a magnifying glass to photos.
I mentioned Adams because I like his pictures. If he had taken them with
a digital camera I would have liked them just as much. I also like some
modern art paintings. That does not mean we should start drawing what we
see in the microscopes rather than taking pictures ;-)

Jim wrote:

'Incidentally, from the outset I cited increased "enlargability" to
obtain high
resolution TEM'

Well, you said 'When great enlargements are required film is superior'.
Perhaps I misunderstood. I thought, the term great enlargements referred
to the microscope and meant 'small details', which you can take easier
with a digital camera (no time delay between seeing something and taking
an image, no mechanical vibration due to film movement, etc.). I have
said many times before that film may be better if high resolution AND
large field of view is required.

Jim wrote:

'in any case: Show Sergey!'

I'm in back-channel correspondence with him.



Michael

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: jim [mailto:jim-at-proscitech.com.au]
Sent: Sunday, June 04, 2000 2:34 AM
To: 'Mike Bode'; 'Microscopy-at-MSA.Microscopy.Com'


I have two replies and Mike my well have four replies to these.
1. There has been a parallel discussion concerning resolution of
film versus
digital images. Clearly in raw power digital cannot compete since film
has
multi gigabyte capacity. I added to that thread that what matters is:
does
digital have enough power and that frequently it would. Mike reinforced
and
strengthened that argument, finishing with the note that he is glad for
film
when looking at prints by Ansel Adams.
(Ansel Adams until about 30 years ago carted for decades large format
cameras
through US National Parks, especially Yosemite, producing fantastic
landscape
photographs and books)
Adams' limited edition prints were contacts of 5x7 and 8x10" inch sheet
film,
probably rated at 400 ISO. The line resolution of such prints much
exceeds our
eyes' resolution, but still results in superior gradation and detail.
TEM film,
even when much enlarged has such details too.
Why Mike, should we accept 2.5 Mb? That is a splendid file size for SEM,
and
because of the limited enlargability of light microscopy (concrete
ceiling due
to wavelengths) it's reasonable, but minimal for light microscopy. TEM
can do
and deserves better.

Incidentally, from the outset I cited increased "enlargability" to
obtain high
resolution TEM, as one of films major advantages, since greater depths
of field
at moderate powers makes high powers through photo enlarging a desirable

technique. The small additional magnification yielded by placing a
digital
camera lower in the column does not compensate. So greater
"enlargability" of
digitals would be desirable, but is limited by pixel size.

2 The thread was initiated by Sergey. He had problems visualising
certain
specimens in dark field. The use of his "beaut" digital TEM camera made
things
worse. I pointed out that the shorter exposure reduced the number of
electrons
forming the image, hence more noise. I believe that a good part of
Mike's case
will be settled in his favour when we hear "Eureka" from Sergey's lab. I
don't
doubt that much more can be done with digital now and that further
improvements
are on the way. Mike's "solution" may well be possible, but I don't
believe its
a snap; in any case: Show Sergey!
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Saturday, June 03, 2000 2:58 AM, Mike Bode [SMTP:mb-at-Soft-Imaging.com]
wrote:
}
} Well, let's see:
}
} Jim wrote:
}
} Mike Bode would use multiple digital
} exposures. The exposures could be layered and combined into one
superior
} image.
} This image would be made up of more pixel and is formed by more
} electrons and
} so would be noise-free and hence could be further enlarged then
} otherwise
} possible. Perhaps.
}
} No, I did not talk about further enlargements. All I wanted to say is,
} that a more noise-free image can be achieved by adding multiple
images,
} and that this also to some extent helps with drift of the sample
during
} acquisition.
}
} Jim wrote:
}
} How much time is required between exposures to transfer a minimum 10mb
} image
} per exposure?
}
} How did you arrive at 10 MB? A 1280x1024 image with 16 bit pixel
} information is about 2.5 MB (uncompressed). We acquire about 10 of
those
} per second and transfer them across the PC bus to the display. Putting
} them on them into Memory might add a few tenth of a second. Writing to
} HD can be done after all images are acquired.
}
} Jim wrote:
}
} What would be the total time from focusing to the last exposure?
} What about Z-drift in the interim requiring objective changes
}
} Why would we have to worry about that, if we don't have to worry about
} that when taking the image on film? In fact, we could take care of
this
} by looking at the image between exposures and correct for z-drift.
} However, as you said, that would add to the overall time and exposure.
I
} was comparing a normal dark field image taken on film at 8 seconds
with
} acquiring the same image on a "too sensitive" CCD camera by adding up
10
} consecutive .8 second images. Why would the sample drift (in x, y or
z)
} substantially more in 8+delta seconds than in 8?
}
} Jim wrote:
}
} what about the total cost of this additional get-up
}
} That of course depends on the microscope and there is no general
answer.
} For example on a LEO 912 I believe the blanker is standard. The
} additional cost to use an acquisition scheme like this with our
software
} is $0 plus perhaps a bit of time to write a small macro. On other
} microscopes one might have to add a beam blanker and perhaps a control
} mechanism for the beam blanker. But I would guess, that this cost is
not
} very high. All modern microscopes are computer controller anyway, so
it
} is most likely just a control command that needs to be sent to the
} microscope over a serial port if the beam blanker is installed. Piece
of
} cake.
}
} Jim wrote:
}
} The mind boggles at a through focus series.
}
} You're right here. But I don't think we were talking about
through-focus
} series. Incidentally, we do through-focus series on light microscopes
} and reconstruction routinely. Takes a few images at different focus
(or
} for a light microscope: stage) settings. The rest is done off-line.
} Takes maybe a couple of minutes for about 20 images of about 1kx1k. I
} agree that TEM is different here and much more complicated due to the
} complicated Contrast Transfer Function. However, this could in
principle
} be sorted out.
}
} Jim wrote:
}
} Again, I don't doubt that there is now a large place for digital in
TEM,
} but
} its no panacea.
}
} I also agree with you on that one. But using the additional computer
} possibilities of digital imaging might take you further than expected.
}
} Michael
}
}
} Michael Bode, Ph.D.
} Soft Imaging System Corp.
} 1675 Carr St., #105N
} Lakewood, CO 80215
} ===================================
} phone: (888) FIND SIS
} (303) 234-9270
} fax: (303) 234-9271
} email: mailto:info-at-soft-imaging.com
} web: http://www.soft-imaging.com
} ===================================
}
}
}
} -----Original Message-----
} } From: jim [mailto:jim-at-proscitech.com.au]
} Sent: Friday, June 02, 2000 5:15 AM
} To: 'Mike Bode'; 'Microscopy-at-MSA.Microscopy.Com'
} Cc: 'jim-at-proscitech.com.au'
} Subject: RE: Film vs Digital
}
}
} The world is full of possible solutions, but are they practical.?
} To produce high-resolution, dark-field or any others TEM images that
} require
} more electrons to form a clear image, Mike Bode would use multiple
} digital
} exposures. The exposures could be layered and combined into one
superior
} image.
} This image would be made up of more pixel and is formed by more
} electrons and
} so would be noise-free and hence could be further enlarged then
} otherwise
} possible. Perhaps.
} Beam blanking would largely save the specimen from beam damage and
drift
} could
} be compensated for by matching up the digitals. Great.
} How much time is required between exposures to transfer a minimum 10mb
} image
} per exposure? What would be the total time from focusing to the last
} exposure?
} What about Z-drift in the interim requiring objective changes and what
} about
} the total cost of this additional get-up. The mind boggles at a
through
} focus
} series.
} When pushing the limits a piece of film seems more effective, cheaper
} and fa
} ster.
} Again, I don't doubt that there is now a large place for digital in
TEM,
} but
} its no panacea.
} Cheers
} Jim Darley
} ProSciTech Microscopy PLUS
} PO Box 111, Thuringowa QLD 4817 Australia
} Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
} Great microscopy catalogue, 500 Links, MSDS, User Notes
} www.proscitech.com
}
} On Friday, June 02, 2000 1:24 AM, Mike Bode [SMTP:mb-at-soft-imaging.com]
} wrote:
} }
} } No, it's not really a problem. It's been done with low density
} } microscopy all the time. Granted, there are some technical aspects
to
} be
} } overcome, but (and I can only speak for ourselves) we have done that
} on
} } a number of microscopes. You are of course correct, that 10 images
at
} } 0.8 seconds take longer than 8 seconds as the image has to be
} } transferred, etc. BUT: that's what beam blankers are for. It is
pretty
} } straightforward to take an image at 0.8 seconds, then blank the beam
} } very quickly before taking the next image. That way you get pretty
} close
} } to the 8 sec total exposure. If there is no beam blanker on the
} } microscope, in most cases it can be added.
} }
} } I am not sure what you mean by "too sensitive". The cameras are
} usually
} } constructed so that 1 electron from the beam creates between a few
} tenth
} } to a few counts (these are all statistical data, of course). The
well
} } width divided by this sensitivity then determines, how many primary
} } electrons are needed to fully expose one pixel. For example, if the
} well
} } width is 50,000 electrons and the sensitivity is 1 count/electron,
one
} } needs 50,000 primary electrons to fill the well. This translates
into
} } roughly a 0.4% statistical error.
} }
} } } From a practical standpoint: You can take images with most cameras
} when
} } the exposure meter on the microscope reads a couple of seconds
without
} } overexposing the camera. On the other hand, you can reduce the
} intensity
} } of the beam until you see single electron events.
} }
} } The one area where CCD cameras may be too sensitive is diffraction.
} The
} } normally huge intensity in the transmitted beam often leads to
} } saturation. In CCDs this can lead to blooming (the intensity spills
} over
} } into neighboring pixels). This can be taken care of with special
chips
} } that have anti-blooming features, but this usually has some other
} } drawbacks. Again, this can also be overcome somewhat with multiple
} } exposures. Film behaves more civilized here, as it simply stops
} } responding to the electrons, but this makes film more or less
useless
} } for quantitative measurements of diffraction patterns. I have done
} } diffraction with CCDs many times and though it does require some
} } tweaking, one can get very good results from them.
} }
} } Michael Bode, Ph.D.
} } Soft Imaging System Corp.
} } 1675 Carr St., #105N
} } Lakewood, CO 80215
} } ===================================
} } phone: (888) FIND SIS
} } (303) 234-9270
} } fax: (303) 234-9271
} } email: mailto:info-at-soft-imaging.com
} } web: http://www.soft-imaging.com
} } ===================================
} }
} }
} }
} } -----Original Message-----
} } From: jim [mailto:jim-at-proscitech.com.au]
} } Sent: Wednesday, May 31, 2000 9:37 PM
} } To: 'Mike Bode'; 'Microscopy-at-MSA.Microscopy.Com'
} } Subject: RE: Film vs Digital
} }
} }
} }
}
------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} } To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} } On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} }
}
-----------------------------------------------------------------------.
} }
} }
} } Yes, it could be done "in theory". Somebody would need to figure out
} the
} }
} } software and perhaps modify the hardware. Then we would find that
the
} } total
} } exposure of the specimen to the electron beam maybe a muliple of the
} } film's
} } exposure. Afterall, an 8 sec film exposure would not amount in
digital
} } to
} } 10x0.8, but we would require considerable time in between exposures.
} } Since the
} } problems in the discussed circumstances are specimen movement and
beam
} } damage,
} } it seems that taking multiple exposures is a poor option.
} }
} } Digital cameras are for some situation too sensitive to electron
} } exposure.
} } Cutting back on electrons is no option since its the electrons that
} form
} } the
} } image in the first instance.
} } Much easier in light microscopy . . . insert a neutral density
} filter.
} } Cheers
} } Jim Darley
} } ProSciTech Microscopy PLUS
} } PO Box 111, Thuringowa QLD 4817 Australia
} } Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
} } Great microscopy catalogue, 500 Links, MSDS, User Notes
} } www.proscitech.com
} }



From daemon Tue Jun 06 23:33:12 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Mon, 5 Jun 2000 10:55:39 -0500
Subject: Re: Administrivia: Resolving power: - Clarification from Nestor

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Jonathon
Low kV is preferred for some biological applications chiefly
because biologists experience difficulty getting enough contrast out
of their specimens. As Dr Alves de Matos rightly says, low kV is
more damaging to specimens than high kV, because the
penetrating power of low-kV electrons is lower and therefore more
beam energy is lost to the specimen. (We think of this exactly the
other way round for SEM, but that's a story for another day!).

The main reason for the apparent shift in biologist's attitudes is that
many are now less concerned with cell and tissue-level
ultrastructure and increasingly interested in imaging
macromolecules. 200kV or 300kV machines are preferred for cryo-
microscopy of macromolecules in vitrified ice because the
resolution is a lot better than at 80 or 120kV, and also because
200kV causes less specimen damage.

However, the cost of resolution either from higher kVs or the
shorter working distance objective lenses favoured by materials
scientists is poorer image contrast. Philips therefore supply
"Biotwin" versions of some of their TEM range which use a long
focal length objective which trading a little resolution for a little
more contrast. This optical configuration is coincidentally very good
for thickish specimens such as ultrathin biological sections where
the ultimate resolution of the machine is pretty much academic
anyway.

Images of thick (#100nm) specimens such as FIB sections of
microelectronic devices seem crisper and more contrasty in our
CM120 Biotwin than in a 200KV machine.
Chris

} From: "A.P.Alves de Matos" {apmatos-at-ip.pt}
To: {Microscopy-at-sparc5.microscopy.com}


Colleagues....

Some of you have misinterpreted my earlier message.
Please feel free to carry on the discussion on film/digitization etc..

I only asked that the "tangental thread" centered about
complaints on a specific product be taken off-line before
any fire and brimstone starts up between a manufacturer
and clients. This is not the forum for that type of feedback.

The previous dicussion should certainly continue as long
as necessary.

Nestor
Your Friendly Neighborhood SysOp



}
} Nestor,
}
} It would be very unfortunate to squelch this important and central debate
} concerning not only the corresponding resolution comparisons but also
} differances in performance due to noise and greyscale depth. New methods of
} enhansing resolution need to be critiqued as well.
}
} Dave Barnard
}
} Wadsworth Center HVEM
} NYS Dept. Health
} Albany NY





From daemon Tue Jun 06 23:33:24 2000



From: Sha Zhu :      szhu-at-engin.umich.edu
Date: Mon, 5 Jun 2000 16:00:52 -0400 (EDT)
Subject: TEM: looking for T-tool

Contents Retrieved from Microscopy Listserver Archives
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We are looking for the "T-tool" - a kind of fixture for TEM sample
polishing. It is from T&T group in USA. We need to know the email
address or telephone number of T&T group.

Any information about the T&T group or the T-tool will be highly
appreciated!

Thanks a lot!

szhu




From daemon Tue Jun 06 23:33:25 2000



From: David Joswiak :      joswiak-at-orca.astro.washington.edu
Date: Mon, 5 Jun 2000 13:30:28 -0700 (PDT)
Subject: Wanted: Used JEOL 2010 TEM

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We are looking to purchase a used JEOL 2010 TEM (LaB6, not FE) in good
working condition. I would appreciate replies from anyone who knows if a
JEOL 2010 is available or will become available in the next 6 months or
so. A STEM unit would increase our interest, but isn't strictly
necessary.

Also, we will be looking for a buyer for our JEOL 1200EX STEM which has
been under service contract from day 1. It has been a highly reliable
microscope and has given years of satisfactory performance.

Dave Joswiak
Research Scientist
Dept. of Astronomy, 351580
University of Washington
Seattle, WA 98195
(206)543-7702
joswiak-at-astro.washington.edu





From daemon Tue Jun 06 23:33:26 2000



From: John J. Bozzola :      bozzola-at-siu.edu
Date: Mon, 5 Jun 2000 16:14:59 -0500
Subject: SEM: softening of beetle elytra

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I am forwarding this request on behalf of Mr. Edoardo Nolfo. If
anyone could be of assistance please send the information to him
directly or (if you prefer) I will forward it to him.

Thank you.

J.B.


} From: Edoardo Nolfo {edoardo.nolfo-at-christ-church.oxford.ac.uk}

} I am a student at the University of Oxford and am writing to ask for some
} advice. I am currently working on multilayer reflectors in beetles; I plan to
} use S.E.M. and T.E.M. to elucidate the fine structure of these reflectors.
} Apparently the elytra of the beetles must be sliced very thinly for S.E.M.
} analysis. My problem lies in the fact that the elytra are extremely
} hard, which
} means they cannot be sliced very thinly. Do you have any advice to
} offer on how
} to soften the elytra for this purpose? Perhaps there is a chemical
} that is used
} to make samples softer, or a standard technique used to soften very hard
} samples. I would be extremely grateful to you if you could help me! Please do
} not hesitate to contact me if you require any more information.
}
} Let me thank you in advance for your help; I look forward to hearing from you
} soon.

####################################################################
John J. Bozzola, Ph.D., Director
Micro-Imaging and Analysis Center
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
####################################################################



From daemon Tue Jun 06 23:33:33 2000



From: John Nailon :      mmjnailo-at-dingo.cc.uq.edu.au
Date: Sun, 04 Jun 2000 08:54:05 +1000
Subject: Re: resolution of Technical Pan

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Chris,
This is because Kodak use EMs to measure the grain size of their silver
halide grains. Tech Pan has a very small grain size (slow film, low
ISO/ASA number) and is therefore capable of greater enlargement than
film with a larger grains. The larger the grain the faster the film
(higher ISO/ASA number).
Regards
JVN
JVN

Chris Jeffree wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} I just came across the following pearl in a 1982 Kodak publication,
} number PDS 61 "Selecting film from Kodak for photomicrography".
}
} "The (Technical Pan) 2415 film has very low granularity and is
} capable of very great enlargement. In some cases the resolving
} power may be beyond that of the microscope image"
}
} How do they know!
} Answers on a postcard please ....
}
} Chris
} =====================================================================
} DR CHRIS JEFFREE
} BIOSEM - BIOLOGICAL SCIENCES EM FACILITY
} UNIVERSITY OF EDINBURGH
} Daniel Rutherford Building
} King's Buildings, Mayfield Road
} EDINBURGH, EH9 3JH, Scotland, UK
} Tel. #44 131 650 5345
} FAX. #44 131 650 6563
} Mobile 0410 585 401
} email c.jeffree-at-ed.ac.uk
} SEM / TEM bookings sem-at-ed.ac.uk
} =====================================================================

--
****************************************************
John V Nailon
Operations Manager
Centre for Microscopy and Microanalysis
The University of Queensland
St. Lucia Queensland 4072
Phone: +61-7-3365-4214
Fax: +61-7-3365-4422
WWW: http://www.uq.edu.au/nanoworld/allstaff.html#Nailon
****************************************************



From daemon Tue Jun 06 23:33:41 2000



From: David Henriks :      Henriks-at-CompuServe.COM
Date: Mon, 5 Jun 2000 21:32:46 -0400
Subject: TEM: looking for T-tool

Contents Retrieved from Microscopy Listserver Archives
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Dear Dr. Zhu:

The "T" tool is a variation of the well known Tripod Polisher¨. We are the
manufacturers of the Tripod Polisher¨ and also manufacture the more compact
BiPod Polisher. The BiPod polisher offers the smaller size as found on the
T-tool while still incorporating our many years of experience in SEM and
TEM cross section polishing as well as our expertise in precision
machining. I know this doesn't help much in locating the T-tool, but it
does offer you the information you need to acquire a tool that will
effectively meet your requirements. If you would like additional
information on these tools, please feel free to contact me.

Best regards-

David
Writing at 6:24:03 PM on 06/05/2000

***************************************************************************
************************

David Henriks
Vice President TEL: 800-728-2233
(toll free in the USA)
South Bay Technology, Inc. +1-949-492-2600
1120 Via Callejon FAX: +1-949-492-1499
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

***************************************************************************
************************

} } } } } Please visit us at http://www.southbaytech.com { { { { {

Manufacturers of precision sample preparation equipment and supplies for
metallography, crystallography and electron microscopy.



Message text written by Sha Zhu
} -----------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America




We are looking for the "T-tool" - a kind of fixture for TEM sample
polishing. It is from T&T group in USA. We need to know the email
address or telephone number of T&T group.

Any information about the T&T group or the T-tool will be highly
appreciated!

Thanks a lot!


szhu

{



From daemon Tue Jun 06 23:33:46 2000



From: Khanh Tran :      khanh-at-deakin.edu.au
Date: Tue, 06 Jun 2000 14:01:40 +1000
Subject: subscribe

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Hello ,

please subscribe
Khanh Tran
Deakin University
662 Blackburn Road
CLAYTON, VIC. 3168
AUSTRALIA



From daemon Tue Jun 06 23:33:52 2000



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Mon, 5 Jun 2000 09:10:09 -0600
Subject: RE: Film vs Digital

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The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Jim wrote:

'I have two replies and Mike my well have four replies to these.'

I'll try. But I agree with you that we should let this thread die. jim,
it seems you're a bit angy at me. If I unintentionally stepped on your
toe I apologize. I did not expect that we would agree 100%, as you are
selling film and I am involved in digital systems. I just hope that some
other readers found some of this useful. I don't consider myself a
"digital fanatic". This film vs. digital issue has many more facets,
some of which we did not even touch, and which can be just as
entertaining.

Jim wrote:

'Mike reinforced and strengthened that argument, finishing with the note
that he is glad for film when looking at prints by Ansel Adams.'

Just for the record: I usually do not take a magnifying glass to photos.
I mentioned Adams because I like his pictures. If he had taken them with
a digital camera I would have liked them just as much. I also like some
modern art paintings. That does not mean we should start drawing what we
see in the microscopes rather than taking pictures ;-)

Jim wrote:

'Incidentally, from the outset I cited increased "enlargability" to
obtain high
resolution TEM'

Well, you said 'When great enlargements are required film is superior'.
Perhaps I misunderstood. I thought, the term great enlargements referred
to the microscope and meant 'small details', which you can take easier
with a digital camera (no time delay between seeing something and taking
an image, no mechanical vibration due to film movement, etc.). I have
said many times before that film may be better if high resolution AND
large field of view is required.

Jim wrote:

'in any case: Show Sergey!'

I'm in back-channel correspondence with him.



Michael

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: jim [mailto:jim-at-proscitech.com.au]
Sent: Sunday, June 04, 2000 2:34 AM
To: 'Mike Bode'; 'Microscopy-at-MSA.Microscopy.Com'


I have two replies and Mike my well have four replies to these.
1. There has been a parallel discussion concerning resolution of
film versus
digital images. Clearly in raw power digital cannot compete since film
has
multi gigabyte capacity. I added to that thread that what matters is:
does
digital have enough power and that frequently it would. Mike reinforced
and
strengthened that argument, finishing with the note that he is glad for
film
when looking at prints by Ansel Adams.
(Ansel Adams until about 30 years ago carted for decades large format
cameras
through US National Parks, especially Yosemite, producing fantastic
landscape
photographs and books)
Adams' limited edition prints were contacts of 5x7 and 8x10" inch sheet
film,
probably rated at 400 ISO. The line resolution of such prints much
exceeds our
eyes' resolution, but still results in superior gradation and detail.
TEM film,
even when much enlarged has such details too.
Why Mike, should we accept 2.5 Mb? That is a splendid file size for SEM,
and
because of the limited enlargability of light microscopy (concrete
ceiling due
to wavelengths) it's reasonable, but minimal for light microscopy. TEM
can do
and deserves better.

Incidentally, from the outset I cited increased "enlargability" to
obtain high
resolution TEM, as one of films major advantages, since greater depths
of field
at moderate powers makes high powers through photo enlarging a desirable

technique. The small additional magnification yielded by placing a
digital
camera lower in the column does not compensate. So greater
"enlargability" of
digitals would be desirable, but is limited by pixel size.

2 The thread was initiated by Sergey. He had problems visualising
certain
specimens in dark field. The use of his "beaut" digital TEM camera made
things
worse. I pointed out that the shorter exposure reduced the number of
electrons
forming the image, hence more noise. I believe that a good part of
Mike's case
will be settled in his favour when we hear "Eureka" from Sergey's lab. I
don't
doubt that much more can be done with digital now and that further
improvements
are on the way. Mike's "solution" may well be possible, but I don't
believe its
a snap; in any case: Show Sergey!
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Saturday, June 03, 2000 2:58 AM, Mike Bode [SMTP:mb-at-Soft-Imaging.com]
wrote:
}
} Well, let's see:
}
} Jim wrote:
}
} Mike Bode would use multiple digital
} exposures. The exposures could be layered and combined into one
superior
} image.
} This image would be made up of more pixel and is formed by more
} electrons and
} so would be noise-free and hence could be further enlarged then
} otherwise
} possible. Perhaps.
}
} No, I did not talk about further enlargements. All I wanted to say is,
} that a more noise-free image can be achieved by adding multiple
images,
} and that this also to some extent helps with drift of the sample
during
} acquisition.
}
} Jim wrote:
}
} How much time is required between exposures to transfer a minimum 10mb
} image
} per exposure?
}
} How did you arrive at 10 MB? A 1280x1024 image with 16 bit pixel
} information is about 2.5 MB (uncompressed). We acquire about 10 of
those
} per second and transfer them across the PC bus to the display. Putting
} them on them into Memory might add a few tenth of a second. Writing to
} HD can be done after all images are acquired.
}
} Jim wrote:
}
} What would be the total time from focusing to the last exposure?
} What about Z-drift in the interim requiring objective changes
}
} Why would we have to worry about that, if we don't have to worry about
} that when taking the image on film? In fact, we could take care of
this
} by looking at the image between exposures and correct for z-drift.
} However, as you said, that would add to the overall time and exposure.
I
} was comparing a normal dark field image taken on film at 8 seconds
with
} acquiring the same image on a "too sensitive" CCD camera by adding up
10
} consecutive .8 second images. Why would the sample drift (in x, y or
z)
} substantially more in 8+delta seconds than in 8?
}
} Jim wrote:
}
} what about the total cost of this additional get-up
}
} That of course depends on the microscope and there is no general
answer.
} For example on a LEO 912 I believe the blanker is standard. The
} additional cost to use an acquisition scheme like this with our
software
} is $0 plus perhaps a bit of time to write a small macro. On other
} microscopes one might have to add a beam blanker and perhaps a control
} mechanism for the beam blanker. But I would guess, that this cost is
not
} very high. All modern microscopes are computer controller anyway, so
it
} is most likely just a control command that needs to be sent to the
} microscope over a serial port if the beam blanker is installed. Piece
of
} cake.
}
} Jim wrote:
}
} The mind boggles at a through focus series.
}
} You're right here. But I don't think we were talking about
through-focus
} series. Incidentally, we do through-focus series on light microscopes
} and reconstruction routinely. Takes a few images at different focus
(or
} for a light microscope: stage) settings. The rest is done off-line.
} Takes maybe a couple of minutes for about 20 images of about 1kx1k. I
} agree that TEM is different here and much more complicated due to the
} complicated Contrast Transfer Function. However, this could in
principle
} be sorted out.
}
} Jim wrote:
}
} Again, I don't doubt that there is now a large place for digital in
TEM,
} but
} its no panacea.
}
} I also agree with you on that one. But using the additional computer
} possibilities of digital imaging might take you further than expected.
}
} Michael
}
}
} Michael Bode, Ph.D.
} Soft Imaging System Corp.
} 1675 Carr St., #105N
} Lakewood, CO 80215
} ===================================
} phone: (888) FIND SIS
} (303) 234-9270
} fax: (303) 234-9271
} email: mailto:info-at-soft-imaging.com
} web: http://www.soft-imaging.com
} ===================================
}
}
}
} -----Original Message-----
} } From: jim [mailto:jim-at-proscitech.com.au]
} Sent: Friday, June 02, 2000 5:15 AM
} To: 'Mike Bode'; 'Microscopy-at-MSA.Microscopy.Com'
} Cc: 'jim-at-proscitech.com.au'
} Subject: RE: Film vs Digital
}
}
} The world is full of possible solutions, but are they practical.?
} To produce high-resolution, dark-field or any others TEM images that
} require
} more electrons to form a clear image, Mike Bode would use multiple
} digital
} exposures. The exposures could be layered and combined into one
superior
} image.
} This image would be made up of more pixel and is formed by more
} electrons and
} so would be noise-free and hence could be further enlarged then
} otherwise
} possible. Perhaps.
} Beam blanking would largely save the specimen from beam damage and
drift
} could
} be compensated for by matching up the digitals. Great.
} How much time is required between exposures to transfer a minimum 10mb
} image
} per exposure? What would be the total time from focusing to the last
} exposure?
} What about Z-drift in the interim requiring objective changes and what
} about
} the total cost of this additional get-up. The mind boggles at a
through
} focus
} series.
} When pushing the limits a piece of film seems more effective, cheaper
} and fa
} ster.
} Again, I don't doubt that there is now a large place for digital in
TEM,
} but
} its no panacea.
} Cheers
} Jim Darley
} ProSciTech Microscopy PLUS
} PO Box 111, Thuringowa QLD 4817 Australia
} Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
} Great microscopy catalogue, 500 Links, MSDS, User Notes
} www.proscitech.com
}
} On Friday, June 02, 2000 1:24 AM, Mike Bode [SMTP:mb-at-soft-imaging.com]
} wrote:
} }
} } No, it's not really a problem. It's been done with low density
} } microscopy all the time. Granted, there are some technical aspects
to
} be
} } overcome, but (and I can only speak for ourselves) we have done that
} on
} } a number of microscopes. You are of course correct, that 10 images
at
} } 0.8 seconds take longer than 8 seconds as the image has to be
} } transferred, etc. BUT: that's what beam blankers are for. It is
pretty
} } straightforward to take an image at 0.8 seconds, then blank the beam
} } very quickly before taking the next image. That way you get pretty
} close
} } to the 8 sec total exposure. If there is no beam blanker on the
} } microscope, in most cases it can be added.
} }
} } I am not sure what you mean by "too sensitive". The cameras are
} usually
} } constructed so that 1 electron from the beam creates between a few
} tenth
} } to a few counts (these are all statistical data, of course). The
well
} } width divided by this sensitivity then determines, how many primary
} } electrons are needed to fully expose one pixel. For example, if the
} well
} } width is 50,000 electrons and the sensitivity is 1 count/electron,
one
} } needs 50,000 primary electrons to fill the well. This translates
into
} } roughly a 0.4% statistical error.
} }
} } } From a practical standpoint: You can take images with most cameras
} when
} } the exposure meter on the microscope reads a couple of seconds
without
} } overexposing the camera. On the other hand, you can reduce the
} intensity
} } of the beam until you see single electron events.
} }
} } The one area where CCD cameras may be too sensitive is diffraction.
} The
} } normally huge intensity in the transmitted beam often leads to
} } saturation. In CCDs this can lead to blooming (the intensity spills
} over
} } into neighboring pixels). This can be taken care of with special
chips
} } that have anti-blooming features, but this usually has some other
} } drawbacks. Again, this can also be overcome somewhat with multiple
} } exposures. Film behaves more civilized here, as it simply stops
} } responding to the electrons, but this makes film more or less
useless
} } for quantitative measurements of diffraction patterns. I have done
} } diffraction with CCDs many times and though it does require some
} } tweaking, one can get very good results from them.
} }
} } Michael Bode, Ph.D.
} } Soft Imaging System Corp.
} } 1675 Carr St., #105N
} } Lakewood, CO 80215
} } ===================================
} } phone: (888) FIND SIS
} } (303) 234-9270
} } fax: (303) 234-9271
} } email: mailto:info-at-soft-imaging.com
} } web: http://www.soft-imaging.com
} } ===================================
} }
} }
} }
} } -----Original Message-----
} } From: jim [mailto:jim-at-proscitech.com.au]
} } Sent: Wednesday, May 31, 2000 9:37 PM
} } To: 'Mike Bode'; 'Microscopy-at-MSA.Microscopy.Com'
} } Subject: RE: Film vs Digital
} }
} }
} }
}
------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} } To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} } On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} }
}
-----------------------------------------------------------------------.
} }
} }
} } Yes, it could be done "in theory". Somebody would need to figure out
} the
} }
} } software and perhaps modify the hardware. Then we would find that
the
} } total
} } exposure of the specimen to the electron beam maybe a muliple of the
} } film's
} } exposure. Afterall, an 8 sec film exposure would not amount in
digital
} } to
} } 10x0.8, but we would require considerable time in between exposures.
} } Since the
} } problems in the discussed circumstances are specimen movement and
beam
} } damage,
} } it seems that taking multiple exposures is a poor option.
} }
} } Digital cameras are for some situation too sensitive to electron
} } exposure.
} } Cutting back on electrons is no option since its the electrons that
} form
} } the
} } image in the first instance.
} } Much easier in light microscopy . . . insert a neutral density
} filter.
} } Cheers
} } Jim Darley
} } ProSciTech Microscopy PLUS
} } PO Box 111, Thuringowa QLD 4817 Australia
} } Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
} } Great microscopy catalogue, 500 Links, MSDS, User Notes
} } www.proscitech.com
} }




From daemon Tue Jun 06 23:33:52 2000



From: Didier Le Thiec :      le_thiec-at-nancy.inra.fr
Date: Tue, 6 Jun 2000 09:09:33 -0500
Subject: leaves

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The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Jonathon
Low kV is preferred for some biological applications chiefly
because biologists experience difficulty getting enough contrast out
of their specimens. As Dr Alves de Matos rightly says, low kV is
more damaging to specimens than high kV, because the
penetrating power of low-kV electrons is lower and therefore more
beam energy is lost to the specimen. (We think of this exactly the
other way round for SEM, but that's a story for another day!).

The main reason for the apparent shift in biologist's attitudes is that
many are now less concerned with cell and tissue-level
ultrastructure and increasingly interested in imaging
macromolecules. 200kV or 300kV machines are preferred for cryo-
microscopy of macromolecules in vitrified ice because the
resolution is a lot better than at 80 or 120kV, and also because
200kV causes less specimen damage.

However, the cost of resolution either from higher kVs or the
shorter working distance objective lenses favoured by materials
scientists is poorer image contrast. Philips therefore supply
"Biotwin" versions of some of their TEM range which use a long
focal length objective which trading a little resolution for a little
more contrast. This optical configuration is coincidentally very good
for thickish specimens such as ultrathin biological sections where
the ultimate resolution of the machine is pretty much academic
anyway.

Images of thick (#100nm) specimens such as FIB sections of
microelectronic devices seem crisper and more contrasty in our
CM120 Biotwin than in a 200KV machine.
Chris

} From: "A.P.Alves de Matos" {apmatos-at-ip.pt}
To: {Microscopy-at-sparc5.microscopy.com}


Dear all,

1) I am working on oak trees, and I would like to describe the
ultrastucture of leaves; also I am looking for a protocol (very detailed)
to do it.
2) On oak leaves I would like to localize the superoxyde
dismutase(s) enzymes by immunocytochemistry, also I am looking for a
protocol (very detailed) to do it.

Thanks in advances.
Dr. Didier Le Thiec

--------------------------------------------
Didier Le Thiec
I.N.R.A. Centre de Recherches Forestieres
Unite d'Ecophysiologie Forestiere
Laboratoire de Pollution Atmospherique
54280 Champenoux - France

Tel : 33 (0)3 83 39 40 98
Fax : 33 (0)3 83 39 40 69
E-mail : le_thiec-at-nancy.inra.fr
http://vectra.nancy.inra.fr/pollu/index.htm
-------------------------------------------




From daemon Tue Jun 06 23:33:57 2000



From: Rafal Dunin-Borkowski :      rafal.db-at-materials.oxford.ac.uk
Date: Tue, 6 Jun 2000 09:26:36 +0100 (BST)
Subject: FEG/TEM one-day meeting, Monday 3 July 2000

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Monday 3 July 2000

New Developments in FEG/TEM

A joint one-day meeting organised by RMS and EMAG

Department of Materials, Oxford University

Following on from the very successful meeting held in 1999, this will
review the current state-of-the-art of the UK's various FEG/TEM
installations in operation.

The meeting will begin with a special keynote lecture on

Advanced TEM Instrumentation for Solving Critical Problems in Materials
Science given by Professor Manfred RŸhle Max-Planck Institute fŸr
Metallforschung, Stuttgart

This lecture will include some news on the new German FEG/TEM project.

There are now ten FEG/TEM facilities in the UK (some with more than one
instrument) and the main programme will consist of a series of invited
presentations from each of the sites, reviewing the latest developments in
instrumentation and covering specialised techniques such as holography,
energy-filtered imaging, spectroscopic imaging, nano-scale analysis, etc.

With JIF, JREI and other special equipment funding now becoming available
it is likely that there will be other sophisticated FEG/TEM installations
in place in the near future. With this in view the meeting will also
include a forward look: what will the next generation of instruments be
like?

Speakers:

John Hutchison (University of Oxford)

Key-note Lecture - Professor Manfred RÄhle (Stuttgart)
Per Bullough (University of Sheffield)
Marin van Heel (Imperial College)
John Berriman (MRC, Cambridge)
Tony Cullis (University of Sheffield)
Ian Jones (University of Birmingham)
Rik Brydson (University of Leeds)
Jeremy Sloan (University of Oxford)
David Cherns (University of Bristol)
Stephen McVitie (Glasgow University)
Stephen Lloyd (University of Cambridge)
John Titchmarsh (University of Oxford)
Paul Midgley (University of Cambridge)


The meeting will conclude with the RMS AGM and Presidential Address.

For further details contact:
Dr Paul Midgley (EMAG): pam33-at-cam.ac.uk or
Dr John Hutchison (RMS): john.hutchison-at-materials.ox.ac.uk



From daemon Tue Jun 06 23:34:11 2000



From: Tobias Baskin :      BaskinT-at-missouri.edu
Date: Tue, 6 Jun 2000 11:06:50 -0400
Subject: Re: resolution of Technical Pan

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Greetings,
Just for the record, Tech Pan is interesting in that you can
vary the grain size over an amzingly wide range by choice of
developer and exposure conditions. You can technidol LC (I think) and
get the teeeny weeeeny grains previously mentioned, or use D19 (I
think) and get really high contrast big grains.

Just my few exposed halides,
Tobias



} Chris,
} This is because Kodak use EMs to measure the grain size of their silver
} halide grains. Tech Pan has a very small grain size (slow film, low
} ISO/ASA number) and is therefore capable of greater enlargement than
} film with a larger grains. The larger the grain the faster the film
} (higher ISO/ASA number).
} Regards
} JVN
} JVN
}
} Chris Jeffree wrote:
} }
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} } I just came across the following pearl in a 1982 Kodak publication,
} } number PDS 61 "Selecting film from Kodak for photomicrography".
} }
} } "The (Technical Pan) 2415 film has very low granularity and is
} } capable of very great enlargement. In some cases the resolving
} } power may be beyond that of the microscope image"
} }
} } How do they know!
} } Answers on a postcard please ....
} }
} } Chris
} } =====================================================================
} } DR CHRIS JEFFREE
} } BIOSEM - BIOLOGICAL SCIENCES EM FACILITY
} } UNIVERSITY OF EDINBURGH
} } Daniel Rutherford Building
} } King's Buildings, Mayfield Road
} } EDINBURGH, EH9 3JH, Scotland, UK
} } Tel. #44 131 650 5345
} } FAX. #44 131 650 6563
} } Mobile 0410 585 401
} } email c.jeffree-at-ed.ac.uk
} } SEM / TEM bookings sem-at-ed.ac.uk
} } =====================================================================
}
} --
} ****************************************************
} John V Nailon
} Operations Manager
} Centre for Microscopy and Microanalysis
} The University of Queensland
} St. Lucia Queensland 4072
} Phone: +61-7-3365-4214
} Fax: +61-7-3365-4422
} WWW: http://www.uq.edu.au/nanoworld/allstaff.html#Nailon
} ****************************************************

_ ____ ^ __ ____ Tobias I. Baskin
/ \ / / \ / \ \ University of Missouri
/ | / / \ \ \ Biological Sciences
/___/ /__ /___ \ \ \__ 109 Tucker Hall
/ / / \ \ \ Columbia, MO 65211-7400 USA
/ / / \ \ \ voice: 573-882-0173
/ /____ / \ \__/ \____ fax: 573-882-0123



From daemon Tue Jun 06 23:34:16 2000



From: Ronald Vane :      RVaneXEI-at-concentric.net
Date: Wednesday, May 24, 2000 10:58 AM
Subject: Re: species analysis in real time

Contents Retrieved from Microscopy Listserver Archives
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MSA Listers:

Two weeks ago I posted a short message about the Ocean Optics spectrometer I
had just received for doing Plasma diagnostics. I tried it and have found it
very useful for examining a plasma cleaning process and optimizing operating
conditions. I purchased a USB 2000 model that plugs into a USB port of a PC
for less than $3000. Ocean Optics, 727-733-2447, www.OceanOptics.com

Ronald Vane
XEI Scientific
NEW WEB SITE! www.SEMCLEAN.com


-----Original Message-----
} From: Ronald Vane {RVaneXEI-at-concentric.net}
To: MSA listserver {Microscopy-at-sparc5.microscopy.com} ; Dr. Gary Gaugler
{gary-at-gaugler.com}



From daemon Tue Jun 06 23:34:18 2000



From: =shAf= :      mshaf-at-darkwing.uoregon.edu
Date: Tue, 6 Jun 2000 10:17:46 -0700
Subject: SEM: agency wants SEM images?

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My SEM facility has just been approached, and in the interest in
providing what they want for a price based on an average, can I query
the SEM community as to what they charge for commercial release of
their SEM imagery. I'm looking for $/image, but if you charge on any
type of sliding scale, I'd be interested in that too. I believe as
well I shouldn't be undercutting commercial facilities, so I'm
especially interested in these numbers.
Reply to me direct, and I'll respond back to the list with the
average, max and min, and no names.

cheerios, =shAf= :o)

{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {}
Michael Shaffer, R.A. - mshaf-at-darkwing.uoregon.edu
Geological Science's Electron Probe Facility - University of Oregon
http://epmalab.uoregon.edu/




From daemon Tue Jun 06 23:34:19 2000



From: anderron-at-us.ibm.com
Date: Tue, 6 Jun 2000 13:32:30 -0400
Subject: Fluorescence in-situ Hybridization (FISH) Course

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This individual contacted the MSA Business Office with the following
request. Please help her if you can, offline. Don't reply back to me.

Thanks,

..snip

Content-Type: text/plain
Content-Disposition: inline

I work for North Carolina State University and we are looking in to
using Fluorescence in-situ Hybridization (FISH) for identification of
bacteria in biofilm. Do you know of any short courses in FISH here in
the USA? I found some out of the country but would prefer to take a
course in USA.

Thanks for your time

Tracey L. Daly {tldaly-at-unity.ncsu.edu}




Ron Anderson, IBM, Hopewell Jct., New York, USA. anderron-at-us.ibm.com

IBM Analytical Services; http://www.chips.ibm.com/services/asg



From daemon Tue Jun 06 23:34:23 2000



From: jmkrupp-at-cats.ucsc.edu (Jon Krupp)
Date: Tue, 6 Jun 2000 11:13:51 -0700
Subject: PGT parts for anyone

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Hi:

I am about to tear the guts out of an old PGT System4 console. I need some
of the electronics, but will toss the rest unless someone would like it.

Jonathan Krupp
Microscopy & Imaging Lab
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu




From daemon Tue Jun 06 23:34:46 2000



From: donald j marshall :      dmrelion-at-world.std.com
Date: Tue, 6 Jun 2000 15:27:35 -0400 (EDT)
Subject: UV optics

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I have an associate who is doing cathodoluminescence studies on materials.
He would like to extend his observations into the UV for a limited number of
tests on a a few samples. He is presently limited by the optics of the
microscope and the fiber optics cable connecting the microscope ocular to
the input of the spectrometer. Does anyone have any suggestions for optical
microscopes with UV optics that might be available for short term lease or
loan that he could consider? Objective magnifications of 5X to 10 X and a 5X
or 10X ocular would probably suffice.

Thanks,

Don Marshall


Donald J. Marshall
Relion Industries
P.O. Box 12
Bedford, MA 01730
Ph: 781-275-4695
FAX: 781-271-0252
email dmrelion-at-world.std.com

Cathodoluminescence, mass spectroscopy, electron beam technology


"A weed is a flower out of place."

(Please note: Do not send email with attachments to this address. Instead, send it to donbarlen-at-aol.com. Thank you.)



From daemon Tue Jun 06 23:34:51 2000



From: Jane Fagerland :      jane.a.fagerland-at-abbott.com
Date: Tue, 6 Jun 2000 16:22:14 -0500
Subject: Position Opening - Biological Electron Microscopy

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The Department of Microscopy and Microanalysis at Abbott Laboratories is
recruiting an Electron Microscopist for its Biological Microscopy group.
This group provides ultrastructural pathology support for Nonclinical Drug
Safety studies, as well as for other biological microscopy projects, such as
cell screening, virus identification and counting, and immunolabeling.

Requirements for this position include:
a Masters' degree in a biological field, such as cell biology, anatomy, or
zoology
a thorough understanding of mammalian histology and ultrastructure
excellent technical skills including tissue collection at necropsy, tissue
and cell processing for TEM, sectioning, staining, operation of electron
microscopes and related equipment, darkroom procedures
ability to communicate information in technical reports and in oral
presentations

Highly desirable, but not essential, are:
experience in ultrastructural pathology or toxicologic pathology
working knowledge of SEM specimen preparation and instrumentation
working knowledge of immunocytochemistry, in situ hybridization, and other
labeling methods at light or electron microscopic level
familiarity with Good Laboratory Practices

We are looking for a team player with outstanding interpersonal skills and
the ability to adjust readily to rapidly changing priorities and shifting
deadlines. The ability to communicate clearly, both verbally and in writing,
is essential. Careful attention to detail and accuracy are required.

The Department of Microscopy and Microanalysis provides corporate-wide
support in Biological and Materials Microscopy to all divisions of Abbott
Laboratories. The facility houses two TEMs (a Philips CM12 STEM and a LEO
910), two SEMs (a Philips XL30-FEG and AMRAY 1830i), three EDXS systems, a
BioRad confocal scanning laser microscope, several fluorescent and light
microscopes (polarized light, DIC), and a Quantimet Image Analysis system.
We also have an Arcturus PixCell laser capture microdissection system and a
Becton Dickinson FACSCalibur flow cytometer. Microtomes include Reichert
Ultracut E and S ultramicrotomes, RMC 6000 XL cryoultrotome, Microm
histological microtome, and Microm HM500 cryostat.

Please send letters of application and resumes to:

Jane A. Fagerland, Ph.D.
Abbott Laboratories
D45M/AP31
200 Abbott Park RD.
Abbott Park IL 60064-6202

(847) 935-0104 voice
(847) 938-5027 fax
jane.a.fagerland -at-abbott.com



From daemon Tue Jun 06 23:34:52 2000



From: Christensen, Kim :      ChristeK-at-whiteoaksemi.com
Date: Tue, 6 Jun 2000 17:42:54 -0400
Subject: Re: T-Tool

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Sha Zhu,

The T-Tool is available from:
Precision TEM, Inc.
Santa Clara, CA
Ph: 408-980-8898
E-mail: liya-at-precisiontem.com

----------------------------------------------------
Kim Christensen
Failure Analysis Laboratory
White Oak Semiconductor
6000 Technology Boulevard
Sandston, Virginia 23150
Ph: 804 952 7307
Fax: 804 952 7902
Pager: 1 800 759 8888, pin# 130 3382
----------------------------------------------------



From daemon Wed Jun 07 08:28:06 2000



From: guerrier-at-ews.uiuc.edu, GUERRGI-at-mail.northgrum.com ()
Date: Tue, 6 Jun 2000 23:43:28 -0500
Subject: Help? Cleaning Optical Lenses

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Email: guerrier-at-ews.uiuc.edu, GUERRGI-at-mail.northgrum.com
Name: Gina Guerrieri

School: University of Illinois


Question: How do you clean a stereo zoom microscope? Specifically, how do
you clean the lenses with out removing them from the system?

---------------------------------------------------------------------------




From daemon Wed Jun 07 08:28:10 2000



From: Kristof Kovacs :      kris-at-almos.vein.hu
Date: Wed, 07 Jun 2000 08:37:32 +0200
Subject: Still alive?

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Dear Microscopists,
I'm just curious if something is wrong on my end or I'm unsuscribed against
my will. I did not receive any posting during the past two-three weeks, and
this is impossible in view of previously received 10-20 postings/day.
Is there any explanation?
Kris
Dr. Kristof Kovacs
Associate Professor
President, Hungarian Society for Microscopy
Phone: +36-(88)-421-684
Fax: +36-(88)-328-643
Mailing Address:
University of Veszprem, P.O.Box 158, Veszprem
H-8201 Hungary



From daemon Wed Jun 07 08:28:28 2000



From: Ian MacLaren :      maclariz-at-yahoo.co.uk
Date: Wed, 7 Jun 2000 08:27:57 +0100 (BST)
Subject: Ceramic cross-section preparation

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Dear all,
I'm currently having a few problems preparing good cross-section specimens
of ceramic thin films on LaAlO3 or NdGaO3 substrates.

I can cut them okay, but thinning them often results in cracking as the
thickness goes below 100 microns. I have a Gatan model 623 disk grinder and
currently have access to SiC abrasive papers to grit sizes of 1500 or 2000,
and also have the Gatan diamond polishing disks and specimen lapping kit.

The problems are as follows:
If we use the 1500 or 2000 grit SiC, we can often get the samples thin
enough, but with poor surface finish which needs to be improved with
dimpling (on both sides), we also risk cracking the sample as SiC papers
often contain imperfections.

Using the Gatan specimen lapping kit, I find that as the disk grinder is
altered to grind off a further 10 microns, the edge of the sample often
catches on the diamond disk and tears some of the diamond coating off,
leaving a lump on the surface which then risks catching the specimen and
cracking it.

I have one possible alternative approach which I used in Sweden last year
(with SiAlON ceramics) involving attaching the sample to a glass slide and
polishing it using diamond spray on non-absorbent paper. I may consider
trying this with these new materials.

Other ideas would be welcome, however, as would suggestions of how to
improve my present method. Please note that I do not have the budget to buy
any expensive new polishing equipment (such as a tripod polisher, for
instance), so the most welcome suggestions would be those that involve
improvements to current techniques, use of different types of polishing
consumables, etc..

I hope to hear several suggestions, both from users and from the companies
who producing polishing and lapping equipment. Why not post them with the
list so we can all benefit from the sharing of experience?

Best wishes

=====
Ian MacLaren
Beijing Laboratory of Electron Microscopy
Chinese Academy of Sciences, P.O. Box 2724
100080 Beijing
China
General Email: ian.maclaren-at-physics.org
Work (esp. large attachments): maclaren-at-image.blem.ac.cn

____________________________________________________________
Do You Yahoo!?
Get your free -at-yahoo.co.uk address at http://mail.yahoo.co.uk
or your free -at-yahoo.ie address at http://mail.yahoo.ie



From daemon Wed Jun 07 08:28:29 2000



From: Petra Wahlbring :      wahlbrin-at-crpgl.lu
Date: Wed, 07 Jun 2000 09:49:03 +0200
Subject: TEM - Searching for Review Article of Biological Applications

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Since I am usually more involved in material analysis I am a little lost on
the biological field. But now I am in the need of a good review article
concerning the application of (analytical) TEM to biological materials. I
am not looking for sophisticated latest developements but rather for basic
applications with some examples, if possible including some examples of
analytical TEM.

Hope to get some input :)

Petra


--------------------------------------------------------------
Dr. Petra Wahlbring
Centre de Recherche Public - Gabriel Lippmann
Laboratoire d'Analyse des Materiaux (LAM)
162a, av. de la Faiencerie L-1511 Luxembourg
tel. +352-466644-402 fax +352-466644-400
e-mail: petra.wahlbring-at-crpgl.lu
Visit our WWW site! http://www.crpgl.lu/~wahlbrin



From daemon Wed Jun 07 08:28:35 2000



From: Gary Coulton :      g.coulton-at-ic.ac.uk
Date: Wed, 07 Jun 2000 12:09:24 +0100
Subject: ICHC 2000

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Dear Friend

ONLY 8 WEEKS TO GO UNTIL ICHC 2000 (3-8 Sept. York University, UK)

Abstracts are still welcome.

Register by the week or combinations of days.

For more details follow the links to the ICHC 2000 web-site below.

Best wishes

Gary Coulton
Dr. Gary Coulton
Molecular Pathology
Division of Biomedical Sciences
Imperial College School of Medicine
The Sir Alexander Fleming Building
South Kensington
London SW7 2AZ

TEL. ++44 020 7594 3190
FAX. ++44 020 7594 3022
e-mail g.coulton-at-ic.ac.uk


-------------------------------------
Announcing the 11th International Congress of Histochemistry and
Cytochemistry (ICHC 2000)

"Cell Biology and Imaging Tools for the New Century"

September 3-8, 2000, York, United Kingdom

ICHC 2000 comprises 27 symposia addressing latest developments and
applications of histochemistry and cytochemistry in the life sciences
including medicine.

Many leading experts to speak

8 weeks to go! Register for the week or by the day!

For further details go to http://www.med.ic.ac.uk/external/ichc_2000

See you there.



From daemon Wed Jun 07 08:28:37 2000



From: jim :      jim-at-proscitech.com.au
Date: Wed, 7 Jun 2000 23:05:26 +1000
Subject: RE: Film vs Digital - the end?

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I was happy to leave things at that, but there are a couple of things that Mike
has raised that need an answer:
Your actual quote concerning Ansel Adams was "Having said that and looking at a
print of Ansel Adams, I am glad there is film, though!!" This is rather at odds
with the new proclamation
} " I mentioned Adams because I like his pictures. If he had taken them with
} a digital camera I would have liked them just as much.
Lots of people have made excellent compositions and many have make technically
superior prints. Adam's has excelled by consistently winning on both account.
Its a bit strange, but Mike I'll remind you why you his prints: It is not just
Adam's great compositions but also the incredible sharpness and tonal range,
which is only possible by huge data density and impossible with a 2.5mb
enlarged print. Digital not only has problems with occasional great demands on
print magnification (pixel size limitations) and lack of electron density (too
short exposures), but because TEM negs, like Adam's prints, are capable of a
terrific tonal range and resolution. An image appears better if theoretical
data minima are exceeded.

I have been a stickler for showing possible conflict of interest. I realize now
that I should have made a disclaimer along the way. I did not, simply because
it never occurred to me that I could have a real or imagined gain. My
aspirations are different, but since we are all subject to self-delusion I'll
add the reality:
We have very little mark-up on film and cannot export beyond New Zealand. For
Australian research institutions money has been very tight and equipment sales
have been low for some years. I doubt that at our exchange rate any digital TEM
cameras will be installed here during the next couple of years at least. I
think that my special friend Chuck is more likely to retain more film sales
than ProSciTech may have lost - if anybody was influenced either way.

The word belief is fitting for all of this. Whatever the arguments, I expect
people retain their "film versus digital" beliefs. Mike, we can lead a horse to
water, but just try to make it float on its back. When you do, send a picture
and I won't care if its digital.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Tuesday, June 06, 2000 1:10 AM, Mike Bode [SMTP:mb-at-soft-imaging.com] wrote:
} Jim wrote:
}
} 'I have two replies and Mike my well have four replies to these.'
}
} I'll try. But I agree with you that we should let this thread die. jim,
} it seems you're a bit angy at me. If I unintentionally stepped on your
} toe I apologize. I did not expect that we would agree 100%, as you are
} selling film and I am involved in digital systems. I just hope that some
} other readers found some of this useful. I don't consider myself a
} "digital fanatic". This film vs. digital issue has many more facets,
} some of which we did not even touch, and which can be just as
} entertaining.
}
} Jim wrote:
}
} 'Mike reinforced and strengthened that argument, finishing with the note
} that he is glad for film when looking at prints by Ansel Adams.'
}
} Just for the record: I usually do not take a magnifying glass to photos.
} I mentioned Adams because I like his pictures. If he had taken them with
} a digital camera I would have liked them just as much. I also like some
} modern art paintings. That does not mean we should start drawing what we
} see in the microscopes rather than taking pictures ;-)
}
} Jim wrote:
}
} 'Incidentally, from the outset I cited increased "enlargability" to
} obtain high
} resolution TEM'
}
} Well, you said 'When great enlargements are required film is superior'.
} Perhaps I misunderstood. I thought, the term great enlargements referred
} to the microscope and meant 'small details', which you can take easier
} with a digital camera (no time delay between seeing something and taking
} an image, no mechanical vibration due to film movement, etc.). I have
} said many times before that film may be better if high resolution AND
} large field of view is required.
}
} Jim wrote:
}
} 'in any case: Show Sergey!'
}
} I'm in back-channel correspondence with him.
}
}
}
} Michael
}
} Michael Bode, Ph.D.
} Soft Imaging System Corp.
} 1675 Carr St., #105N
} Lakewood, CO 80215
} ===================================
} phone: (888) FIND SIS
} (303) 234-9270
} fax: (303) 234-9271
} email: mailto:info-at-soft-imaging.com
} web: http://www.soft-imaging.com
} ===================================
}
}
}
} -----Original Message-----
} From: jim [mailto:jim-at-proscitech.com.au]
} Sent: Sunday, June 04, 2000 2:34 AM
} To: 'Mike Bode'; 'Microscopy-at-MSA.Microscopy.Com'
} Subject: RE: Film vs Digital
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I have two replies and Mike my well have four replies to these.
} 1. There has been a parallel discussion concerning resolution of
} film versus
} digital images. Clearly in raw power digital cannot compete since film
} has
} multi gigabyte capacity. I added to that thread that what matters is:
} does
} digital have enough power and that frequently it would. Mike reinforced
} and
} strengthened that argument, finishing with the note that he is glad for
} film
} when looking at prints by Ansel Adams.
} (Ansel Adams until about 30 years ago carted for decades large format
} cameras
} through US National Parks, especially Yosemite, producing fantastic
} landscape
} photographs and books)
} Adams' limited edition prints were contacts of 5x7 and 8x10" inch sheet
} film,
} probably rated at 400 ISO. The line resolution of such prints much
} exceeds our
} eyes' resolution, but still results in superior gradation and detail.
} TEM film,
} even when much enlarged has such details too.
} Why Mike, should we accept 2.5 Mb? That is a splendid file size for SEM,
} and
} because of the limited enlargability of light microscopy (concrete
} ceiling due
} to wavelengths) it's reasonable, but minimal for light microscopy. TEM
} can do
} and deserves better.
}
} Incidentally, from the outset I cited increased "enlargability" to
} obtain high
} resolution TEM, as one of films major advantages, since greater depths
} of field
} at moderate powers makes high powers through photo enlarging a desirable
}
} technique. The small additional magnification yielded by placing a
} digital
} camera lower in the column does not compensate. So greater
} "enlargability" of
} digitals would be desirable, but is limited by pixel size.
}
} 2 The thread was initiated by Sergey. He had problems visualising
} certain
} specimens in dark field. The use of his "beaut" digital TEM camera made
} things
} worse. I pointed out that the shorter exposure reduced the number of
} electrons
} forming the image, hence more noise. I believe that a good part of
} Mike's case
} will be settled in his favour when we hear "Eureka" from Sergey's lab. I
} don't
} doubt that much more can be done with digital now and that further
} improvements
} are on the way. Mike's "solution" may well be possible, but I don't
} believe its
} a snap; in any case: Show Sergey!
} Cheers
} Jim Darley
} ProSciTech Microscopy PLUS
} PO Box 111, Thuringowa QLD 4817 Australia
} Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
} Great microscopy catalogue, 500 Links, MSDS, User Notes
} www.proscitech.com
}
} On Saturday, June 03, 2000 2:58 AM, Mike Bode [SMTP:mb-at-Soft-Imaging.com]
} wrote:
} }
} } Well, let's see:
} }
} } Jim wrote:
} }
} } Mike Bode would use multiple digital
} } exposures. The exposures could be layered and combined into one
} superior
} } image.
} } This image would be made up of more pixel and is formed by more
} } electrons and
} } so would be noise-free and hence could be further enlarged then
} } otherwise
} } possible. Perhaps.
} }
} } No, I did not talk about further enlargements. All I wanted to say is,
} } that a more noise-free image can be achieved by adding multiple
} images,
} } and that this also to some extent helps with drift of the sample
} during
} } acquisition.
} }
} } Jim wrote:
} }
} } How much time is required between exposures to transfer a minimum 10mb
} } image
} } per exposure?
} }
} } How did you arrive at 10 MB? A 1280x1024 image with 16 bit pixel
} } information is about 2.5 MB (uncompressed). We acquire about 10 of
} those
} } per second and transfer them across the PC bus to the display. Putting
} } them on them into Memory might add a few tenth of a second. Writing to
} } HD can be done after all images are acquired.
} }
} } Jim wrote:
} }
} } What would be the total time from focusing to the last exposure?
} } What about Z-drift in the interim requiring objective changes
} }
} } Why would we have to worry about that, if we don't have to worry about
} } that when taking the image on film? In fact, we could take care of
} this
} } by looking at the image between exposures and correct for z-drift.
} } However, as you said, that would add to the overall time and exposure.
} I
} } was comparing a normal dark field image taken on film at 8 seconds
} with
} } acquiring the same image on a "too sensitive" CCD camera by adding up
} 10
} } consecutive .8 second images. Why would the sample drift (in x, y or
} z)
} } substantially more in 8+delta seconds than in 8?
} }
} } Jim wrote:
} }
} } what about the total cost of this additional get-up
} }
} } That of course depends on the microscope and there is no general
} answer.
} } For example on a LEO 912 I believe the blanker is standard. The
} } additional cost to use an acquisition scheme like this with our
} software
} } is $0 plus perhaps a bit of time to write a small macro. On other
} } microscopes one might have to add a beam blanker and perhaps a control
} } mechanism for the beam blanker. But I would guess, that this cost is
} not
} } very high. All modern microscopes are computer controller anyway, so
} it
} } is most likely just a control command that needs to be sent to the
} } microscope over a serial port if the beam blanker is installed. Piece
} of
} } cake.
} }
} } Jim wrote:
} }
} } The mind boggles at a through focus series.
} }
} } You're right here. But I don't think we were talking about
} through-focus
} } series. Incidentally, we do through-focus series on light microscopes
} } and reconstruction routinely. Takes a few images at different focus
} (or
} } for a light microscope: stage) settings. The rest is done off-line.
} } Takes maybe a couple of minutes for about 20 images of about 1kx1k. I
} } agree that TEM is different here and much more complicated due to the
} } complicated Contrast Transfer Function. However, this could in
} principle
} } be sorted out.
} }
} } Jim wrote:
} }
} } Again, I don't doubt that there is now a large place for digital in
} TEM,
} } but
} } its no panacea.
} }
} } I also agree with you on that one. But using the additional computer
} } possibilities of digital imaging might take you further than expected.
} }
} } Michael
} }
} }
} } Michael Bode, Ph.D.
} } Soft Imaging System Corp.
} } 1675 Carr St., #105N
} } Lakewood, CO 80215
} } ===================================
} } phone: (888) FIND SIS
} } (303) 234-9270
} } fax: (303) 234-9271
} } email: mailto:info-at-soft-imaging.com
} } web: http://www.soft-imaging.com
} } ===================================
} }
} }
} }
} } -----Original Message-----
} } } From: jim [mailto:jim-at-proscitech.com.au]
} } Sent: Friday, June 02, 2000 5:15 AM
} } To: 'Mike Bode'; 'Microscopy-at-MSA.Microscopy.Com'
} } Cc: 'jim-at-proscitech.com.au'
} } Subject: RE: Film vs Digital
} }
} }
} } The world is full of possible solutions, but are they practical.?
} } To produce high-resolution, dark-field or any others TEM images that
} } require
} } more electrons to form a clear image, Mike Bode would use multiple
} } digital
} } exposures. The exposures could be layered and combined into one
} superior
} } image.
} } This image would be made up of more pixel and is formed by more
} } electrons and
} } so would be noise-free and hence could be further enlarged then
} } otherwise
} } possible. Perhaps.
} } Beam blanking would largely save the specimen from beam damage and
} drift
} } could
} } be compensated for by matching up the digitals. Great.
} } How much time is required between exposures to transfer a minimum 10mb
} } image
} } per exposure? What would be the total time from focusing to the last
} } exposure?
} } What about Z-drift in the interim requiring objective changes and what
} } about
} } the total cost of this additional get-up. The mind boggles at a
} through
} } focus
} } series.
} } When pushing the limits a piece of film seems more effective, cheaper
} } and fa
} } ster.
} } Again, I don't doubt that there is now a large place for digital in
} TEM,
} } but
} } its no panacea.
} } Cheers
} } Jim Darley
} } ProSciTech Microscopy PLUS
} } PO Box 111, Thuringowa QLD 4817 Australia
} } Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
} } Great microscopy catalogue, 500 Links, MSDS, User Notes
} } www.proscitech.com
} }
} } On Friday, June 02, 2000 1:24 AM, Mike Bode [SMTP:mb-at-soft-imaging.com]
} } wrote:
} } }
} } } No, it's not really a problem. It's been done with low density
} } } microscopy all the time. Granted, there are some technical aspects
} to
} } be
} } } overcome, but (and I can only speak for ourselves) we have done that
} } on
} } } a number of microscopes. You are of course correct, that 10 images
} at
} } } 0.8 seconds take longer than 8 seconds as the image has to be
} } } transferred, etc. BUT: that's what beam blankers are for. It is
} pretty
} } } straightforward to take an image at 0.8 seconds, then blank the beam
} } } very quickly before taking the next image. That way you get pretty
} } close
} } } to the 8 sec total exposure. If there is no beam blanker on the
} } } microscope, in most cases it can be added.
} } }
} } } I am not sure what you mean by "too sensitive". The cameras are
} } usually
} } } constructed so that 1 electron from the beam creates between a few
} } tenth
} } } to a few counts (these are all statistical data, of course). The
} well
} } } width divided by this sensitivity then determines, how many primary
} } } electrons are needed to fully expose one pixel. For example, if the
} } well
} } } width is 50,000 electrons and the sensitivity is 1 count/electron,
} one
} } } needs 50,000 primary electrons to fill the well. This translates
} into
} } } roughly a 0.4% statistical error.
} } }
} } } } From a practical standpoint: You can take images with most cameras
} } when
} } } the exposure meter on the microscope reads a couple of seconds
} without
} } } overexposing the camera. On the other hand, you can reduce the
} } intensity
} } } of the beam until you see single electron events.
} } }
} } } The one area where CCD cameras may be too sensitive is diffraction.
} } The
} } } normally huge intensity in the transmitted beam often leads to
} } } saturation. In CCDs this can lead to blooming (the intensity spills
} } over
} } } into neighboring pixels). This can be taken care of with special
} chips
} } } that have anti-blooming features, but this usually has some other
} } } drawbacks. Again, this can also be overcome somewhat with multiple
} } } exposures. Film behaves more civilized here, as it simply stops
} } } responding to the electrons, but this makes film more or less
} useless
} } } for quantitative measurements of diffraction patterns. I have done
} } } diffraction with CCDs many times and though it does require some
} } } tweaking, one can get very good results from them.
} } }
} } } Michael Bode, Ph.D.
} } } Soft Imaging System Corp.
} } } 1675 Carr St., #105N
} } } Lakewood, CO 80215
} } } ===================================
} } } phone: (888) FIND SIS
} } } (303) 234-9270
} } } fax: (303) 234-9271
} } } email: mailto:info-at-soft-imaging.com
} } } web: http://www.soft-imaging.com
} } } ===================================
} } }
} } }
} } }
} } } -----Original Message-----
} } } From: jim [mailto:jim-at-proscitech.com.au]
} } } Sent: Wednesday, May 31, 2000 9:37 PM
} } } To: 'Mike Bode'; 'Microscopy-at-MSA.Microscopy.Com'
} } } Subject: RE: Film vs Digital
} } }
} } }
} } }
} }
} ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} } America
} } } To Subscribe/Unsubscribe -- Send Email to
} } ListServer-at-MSA.Microscopy.Com
} } } On-Line Help
} } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } }
} }
} -----------------------------------------------------------------------.
} } }
} } }
} } } Yes, it could be done "in theory". Somebody would need to figure out
} } the
} } }
} } } software and perhaps modify the hardware. Then we would find that
} the
} } } total
} } } exposure of the specimen to the electron beam maybe a muliple of the
} } } film's
} } } exposure. Afterall, an 8 sec film exposure would not amount in
} digital
} } } to
} } } 10x0.8, but we would require considerable time in between exposures.
} } } Since the
} } } problems in the discussed circumstances are specimen movement and
} beam
} } } damage,
} } } it seems that taking multiple exposures is a poor option.
} } }
} } } Digital cameras are for some situation too sensitive to electron
} } } exposure.
} } } Cutting back on electrons is no option since its the electrons that
} } form
} } } the
} } } image in the first instance.
} } } Much easier in light microscopy . . . insert a neutral density
} } filter.
} } } Cheers
} } } Jim Darley
} } } ProSciTech Microscopy PLUS
} } } PO Box 111, Thuringowa QLD 4817 Australia
} } } Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
} } } Great microscopy catalogue, 500 Links, MSDS, User Notes
} } } www.proscitech.com
} } }



From daemon Wed Jun 07 08:28:37 2000



From: Walck, Scott D. :      walck-at-ppg.com
Date: Wed, 7 Jun 2000 09:17:56 -0400
Subject: RE: Ceramic cross-section preparation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


This sounds like a perfect application of the small angle cleavage
technique. You might want to contact Ray Tweston at Univ. of Illinois
because I think that we might have successfully prepared one of these while
I visited there. At any rate, the technique is very inexpensive and you can
prepare sample of superior quality. Get your hands on John McCaffrey and my
paper in the MRS TEM sample Prep IV book (vol 480). It has a detailed
description on how to do it. South Bay Technology sells the Microcleave kit
and you should contact them as well.


-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)



} -----Original Message-----
} From: Ian MacLaren [mailto:maclariz-at-yahoo.co.uk]
} Sent: Wednesday, June 07, 2000 3:28 AM
} To: Microscopy list
} Subject: Ceramic cross-section preparation
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} ---------.
}
}
} Dear all,
} I'm currently having a few problems preparing good
} cross-section specimens
} of ceramic thin films on LaAlO3 or NdGaO3 substrates.
}
} I can cut them okay, but thinning them often results in
} cracking as the
} thickness goes below 100 microns. I have a Gatan model 623
} disk grinder and
} currently have access to SiC abrasive papers to grit sizes of
} 1500 or 2000,
} and also have the Gatan diamond polishing disks and specimen
} lapping kit.
}
} The problems are as follows:
} If we use the 1500 or 2000 grit SiC, we can often get the
} samples thin
} enough, but with poor surface finish which needs to be improved with
} dimpling (on both sides), we also risk cracking the sample as
} SiC papers
} often contain imperfections.
}
} Using the Gatan specimen lapping kit, I find that as the disk
} grinder is
} altered to grind off a further 10 microns, the edge of the
} sample often
} catches on the diamond disk and tears some of the diamond
} coating off,
} leaving a lump on the surface which then risks catching the
} specimen and
} cracking it.
}
} I have one possible alternative approach which I used in
} Sweden last year
} (with SiAlON ceramics) involving attaching the sample to a
} glass slide and
} polishing it using diamond spray on non-absorbent paper. I
} may consider
} trying this with these new materials.
}
} Other ideas would be welcome, however, as would suggestions of how to
} improve my present method. Please note that I do not have
} the budget to buy
} any expensive new polishing equipment (such as a tripod polisher, for
} instance), so the most welcome suggestions would be those
} that involve
} improvements to current techniques, use of different types of
} polishing
} consumables, etc..
}
} I hope to hear several suggestions, both from users and from
} the companies
} who producing polishing and lapping equipment. Why not post
} them with the
} list so we can all benefit from the sharing of experience?
}
} Best wishes
}
} =====
} Ian MacLaren
} Beijing Laboratory of Electron Microscopy
} Chinese Academy of Sciences, P.O. Box 2724
} 100080 Beijing
} China
} General Email: ian.maclaren-at-physics.org
} Work (esp. large attachments): maclaren-at-image.blem.ac.cn
}
} ____________________________________________________________
} Do You Yahoo!?
} Get your free -at-yahoo.co.uk address at http://mail.yahoo.co.uk
} or your free -at-yahoo.ie address at http://mail.yahoo.ie
}



From daemon Wed Jun 07 08:57:58 2000



From: Judith C. Yang :      jyang-at-engrng.pitt.edu (by way of Nestor J.
Date: Wed, 7 Jun 2000 08:34:48 -0500
Subject: post-doc opening

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Postdoctoral Position Immediately Available
In situ UHV-TEM of Nanoparticle Reactions and Metal Oxidation

Materials Research Laboratory, University of Illinois at
Urbana-Champaign
and University of Pittsburgh

A postdoctoral position is immediately available in the Materials
Research
Laboratory at the University of Illinois at Urbana-Champaign in the area
of
nano-reactions and in situ UHV-TEM. This position is jointly sponsored
between
Professor Robert Averback (University of Illinois at Urbana-Champaign)
and
Professor Judith Yang (University of Pittsburgh).

The research project is two-fold: 1. Surface oxidation kinetics of
metals
and 2. Nanoparticle reactions/sintering. The research project is to
combine the unique experimental information obtainable from in situ
UHV-TEM
and compare with
theoretical models of these nanoscale reactions. The position requires
a
PhD in physical sciences/engineering. Hands-on experience in TEM
techniques
is highly desirable. The position is open to all qualified candidates
and
has an anticipated duration of 2 years. If you are interested in this
opportunity, please send a resume and names of three references to the
address below.


Dr. Judith C. Yang
Assistant Professor
Dept. of Materials Science & Engineering
848 Benedum Hall
University of Pittsburgh
Pittsburgh, PA 15261

Tel: (412) 624-8613
Fax: (412) 624-8069
jyang-at-engrng.pitt.edu




From daemon Wed Jun 07 08:57:59 2000



From: Corneliu Sarbu :      Corneliu.Sarbu-at-mtm.kuleuven.ac.be
Date: Wed, 7 Jun 2000 08:37:34 -0500
Subject: re: TEM - Searching for Review Article of Biological Applicatio

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I can reccomend you the following books:
1. D.C.Joy, A.D.Romig and J.I.Goldstein, "Principles of analytical
electron microscopy", Plenum Press, 1989;
it contains three chapters dedicated to bilogy: chapter 6, 12 and 13
2. J.J.Hren, J.I.Goldstein and D.C.Joy, "Introduction to AEM",
Plenum Press, 1979;
it contains chapters dedicated to biology.
I hope this helps.

Corneliu Sarbu
Dept.MTM
KULeuven
Belgium

- - - - - - - - - - - - - - Original Message - - - - - - - - - - - - - -
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America




Since I am usually more involved in material analysis I am a little lost on
the biological field. But now I am in the need of a good review article
concerning the application of (analytical) TEM to biological materials. I
am not looking for sophisticated latest developements but rather for basic
applications with some examples, if possible including some examples of
analytical TEM.

Hope to get some input :)

Petra


--------------------------------------------------------------
Dr. Petra Wahlbring
Centre de Recherche Public - Gabriel Lippmann
Laboratoire d'Analyse des Materiaux (LAM)
162a, av. de la Faiencerie L-1511 Luxembourg
tel. +352-466644-402 fax +352-466644-400
e-mail: petra.wahlbring-at-crpgl.lu
Visit our WWW site! http://www.crpgl.lu/~wahlbrin


- - - - - - - - - - - - End of Original Message - - - - - - - - - - - -




From daemon Wed Jun 07 18:36:38 2000



From: Kim Pierson :      piersokw-at-uwec.edu
Date: Wed, 07 Jun 2000 09:03:26 -0500
Subject: JEOL 2010 LaB6 Question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Question:
What is the proper procedure for increasing the filament current on a 2010
with a LaB6?
How fast can you go up?
Should the current be increased at a linear rate or should the rate be
tapered off as you reach saturation?
The manual suggests 30 sec/graduation while the service techs suggest a
much slower rate.

Thanks,
Kim
**************************************************************
Kim W. Pierson, Ph.D.
Dept of Physics & Astronmoy
University of Wisconsin-Eau Claire
(715) 836-5009
FAX 836-3955



From daemon Wed Jun 07 18:36:38 2000



From: David Henriks :      Henriks-at-CompuServe.COM
Date: Wed, 7 Jun 2000 10:41:19 -0400
Subject: Ceramic cross-section preparation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Ian:

I have a few comments for you here that may help. This is a little touchy
as I don't want to knock my competitor's equipment. The Model 623 Disk
Grinder that you have is going to cause problems as you describe because of
it's design. The way that grinder works is that you use the dial on the
top to make your sample extend below the base of the grinder. When you
begin polishing, the entire weight of the grinder plus whatever weight you
are applying by hand is being transferred to your very thin 3mm diameter
sample. In addition to the additional weight, you can tend to apply too
much pressure on one side of the sample which would cause the sample to
"catch" on the diamond film as you describe.

We manufacture a series of polishing fixtures that are designed to prevent
these problems. They are gravity feed fixtures. On these fixtures, the
dial gauge at the top simply sets the amount of material that will be fed
into the abrasive film. The only weight your sample sees is the weight of
the central piston (with some of our fixtures, this weight can be
counterbalanced to approach zero). The weight of the rest of the fixture
is never transferred to the sample. Also, the sample surface that is being
polished is always co-planar with the base of the polishing fixture. This
ensures that you cannot apply uneven pressure and that you won't experience
the "catching" that you described.

Now the good news. There is one of these fixtures (Model 150) already in
use in your facility. There is also the Tripod Polisher¨, Model 920
Lapping & Polishing Machine, Model 850 Wire Saw and Model 650 Diamond Wheel
Saw. By separate e-mail,I will give you the details on where these are
located and who you should contact to access them.

I also saw Scott Walck's post on the Microcleave technique. I will include
a PDF of the paper he was talking about by separate e-mail.

If you have any questions or if I can be of any additional assistance,
please contact me directly off-line. I hope this helps.

DISCLAIMER: South Bay Technology produces equipment and supplies as
described above and, therefore, has a vested interest in promoting their
use.

Best regards-

David
Writing at 7:20:30 AM on 06/07/2000

***************************************************************************
************************

David Henriks
Vice President TEL: 800-728-2233
(toll free in the USA)
South Bay Technology, Inc. +1-949-492-2600
1120 Via Callejon FAX: +1-949-492-1499
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

***************************************************************************
************************

} } } } } Please visit us at http://www.southbaytech.com { { { { {

Manufacturers of precision sample preparation equipment and supplies for
metallography, crystallography and electron microscopy.
Message text written by INTERNET:ian.maclaren-at-physics.org
}
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Dear all,
I'm currently having a few problems preparing good cross-section specimens
of ceramic thin films on LaAlO3 or NdGaO3 substrates.

I can cut them okay, but thinning them often results in cracking as the
thickness goes below 100 microns. I have a Gatan model 623 disk grinder
and
currently have access to SiC abrasive papers to grit sizes of 1500 or 2000,

and also have the Gatan diamond polishing disks and specimen lapping kit.

The problems are as follows:
If we use the 1500 or 2000 grit SiC, we can often get the samples thin
enough, but with poor surface finish which needs to be improved with
dimpling (on both sides), we also risk cracking the sample as SiC papers
often contain imperfections.

Using the Gatan specimen lapping kit, I find that as the disk grinder is
altered to grind off a further 10 microns, the edge of the sample often
catches on the diamond disk and tears some of the diamond coating off,
leaving a lump on the surface which then risks catching the specimen and
cracking it.

I have one possible alternative approach which I used in Sweden last year
(with SiAlON ceramics) involving attaching the sample to a glass slide and
polishing it using diamond spray on non-absorbent paper. I may consider
trying this with these new materials.

Other ideas would be welcome, however, as would suggestions of how to
improve my present method. Please note that I do not have the budget to
buy
any expensive new polishing equipment (such as a tripod polisher, for
instance), so the most welcome suggestions would be those that involve
improvements to current techniques, use of different types of polishing
consumables, etc..

I hope to hear several suggestions, both from users and from the companies
who producing polishing and lapping equipment. Why not post them with the
list so we can all benefit from the sharing of experience?

Best wishes

=====
Ian MacLaren {



From daemon Wed Jun 07 18:36:39 2000



From: Mark West :      mwest-at-ifcsun1.ifisiol.unam.mx
Date: Wed, 7 Jun 2000 09:50:51 -0600 (Hora est‡ndar de MŽxico)
Subject: plant vesicles

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

I'm trying to do immunogold studies on a membrane protein in a preparation
of plant vesicles (from minus 80¼C storage). I did an Epon embedding with
standard procedure (glut and osmium) to check the ultrastructure and got
decent-looking vesicles, but side by side an LR White prep (0.5% glut, 2%
paraformaldehyde, without osmium) looks like a disaster, with granular
material, vague membranish-like structures and whorls of membranes. Does
anyone have any tips for LR White preps of vesicles, or any other ideas
for embedding (resins, fixing, dehydration, embedding) to favour the
immunogold process.

Thanks,

Mark






********************************************
Mark West,
Unidad de Microscopia Electronica,
(Electron Microscopy Unit)
Instituto de Fisiologia Celular,
Universidad Nacional Autonoma de Mexico,
04510 Mexico D.F.

tel (unidad/lab) *(525) 622 5610*
(casa/home) (525) 619 3020
Fax (525) 616 2282
********************************************



From daemon Wed Jun 07 18:36:47 2000



From: Michelle.Taurino-at-aventis.com
Date: Wed, 7 Jun 2000 11:59:26 -0500
Subject: TEM - Searching for Review Article of Biological Applications

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Petra-

A book that I have found to be very resourceful is
Biomedical Electron Microscopy by Arvid B. Maunsbach and Bjorn Afzelius.

Hope this is useful to you as well.
Regards-
Michelle Taurino
Aventis Pharmaceuticals
Bioimaging and Molecular Histology
Tel-908-231-3357
Fax-908-231-3962
e-mail: Michelle.Taurino-at-aventis.com


-----Original Message-----
} From: Petra Wahlbring [mailto:wahlbrin-at-crpgl.lu]
Sent: Wednesday, June 07, 2000 3:49 AM
To: microscopy-at-sparc5.microscopy.com




Since I am usually more involved in material analysis I am a little lost on
the biological field. But now I am in the need of a good review article
concerning the application of (analytical) TEM to biological materials. I
am not looking for sophisticated latest developements but rather for basic
applications with some examples, if possible including some examples of
analytical TEM.

Hope to get some input :)

Petra


--------------------------------------------------------------
Dr. Petra Wahlbring
Centre de Recherche Public - Gabriel Lippmann
Laboratoire d'Analyse des Materiaux (LAM)
162a, av. de la Faiencerie L-1511 Luxembourg
tel. +352-466644-402 fax +352-466644-400
e-mail: petra.wahlbring-at-crpgl.lu
Visit our WWW site! http://www.crpgl.lu/~wahlbrin



From daemon Wed Jun 07 18:36:48 2000



From: Caroline Schooley :      schooley-at-mcn.org
Date: Wed, 7 Jun 2000 10:01:04 -0700
Subject: Re: SEM: agency wants SEM images?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} My SEM facility has just been approached, and in the interest in
} providing what they want for a price based on an average, can I query
} the SEM community as to what they charge for commercial release of
} their SEM imagery. I'm looking for $/image, but if you charge on any
} type of sliding scale, I'd be interested in that too. I believe as
} well I shouldn't be undercutting commercial facilities, so I'm
} especially interested in these numbers.
} Reply to me direct, and I'll respond back to the list with the
} average, max and min, and no names.

shAf-

Please start with an inquiry to your university administration; you might
save yourself a lot of grief. When I was running an interdepartmental lab
at U.C. Berkeley, there were very specific university-wide rules that I was
required to follow for such usage.

Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html




From daemon Wed Jun 07 18:36:56 2000



From: Marti, Jordi :      jordi.marti-at-honeywell.com
Date: Wed, 7 Jun 2000 13:02:55 -0700
Subject: RE: JEOL 2010 LaB6 Question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Kim:

We have a 2010 and our procedure is to bring the filament dial (labeled
off-10) to position #3. Then we go for coffee. After 20 min. or so we raise
it to # 4, after a couple of minutes to #5 etc. until we get to the stop (
in our case we have it at about 7 1/2). Even at this point we have filament
drift for quite some time, so , we are not ready to take pictures for
another 1/2 hr or so. By the way, we have the bias settings at 7 and 7 which
is high. I would be interested in knowing what your settings are.

I hope this helps

Jordi Marti




From daemon Wed Jun 07 18:36:56 2000



From: Larry Stoter :      LPS-at-teknesis.demon.co.uk
Date: Wed, 7 Jun 2000 20:48:55 +0100
Subject: Re: TEM:beam damage vs voltage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 14:19 +0200 3/6/00, Jonathan Barnard wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

My understanding is as follows ....

There are different damage mechnisms at different voltages.

At lower voltages ( {200 kV) the main damage mechanism is, in effect,
specimen heating. And, the lower the voltage, the greater the
interaction (elastic scattering) with the specimen - so more damage
but greater contrast, the main reason users looking at resin sections
prefer lower voltages.

Increasing the voltage reduces specimen damage and tends to improve
resolution but at the expense of contrast. As the interest in
biological TEM moves to molecular biology, resolution is more
important. But, at high resolution, phase contrast becomes the
dominant factor in producing image constrast and phase constrast can
be significantly improved - without loss of resolution - by using
highly coherent FEG sources.

At voltages between 300kV and 400 kV, the energy of the electron
becomes sufficient to cause "direct" radiation damage - atoms are
displaced. At this point, specimen damage rates increase. The
particular voltage depends on the amtomic number of your specimen.

Damage rates can also be reduced by cooling. in particular, cooling a
specimen to liquid helium temperatures allows significantly greater
electron exposure before damage occurs.

So, for optimum imaging of molecular specimens you need 300 kV and a
FEG gun plus a liquid helium stage.

Regards,
--
Larry Stoter
JEOL (UK) Ltd
Silver Court, Watchmead, Welwyn Garden City, AL7 1LT, United Kingdom
tel: +44-(0)1707-377117, fax: +44-(0)1707-373254, e-mail: larrys-at-jeoleuro.com



From daemon Wed Jun 07 18:37:00 2000



From: Dusevich, Vladimir :      DusevichV-at-umkc.edu
Date: Wed, 7 Jun 2000 17:11:48 -0500
Subject: RE: NEED HELP WITH EDS ON A JEOL 100S

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


For EDS checking you can use specimen
from Cu or Al foil so that it
will have any angle, including 30-60
degrees. But I am afraid that if there
is no spectra at all with 10 degree
tilt then a problem is more complicated.

Vladimir

Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
3127 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy

} -----Original Message-----
} From: "MGMANDERS-at-aol.com"-at-sparc5.microscopy.com
} [mailto:"MGMANDERS-at-aol.com"-at-sparc5.microscopy.com]
} Sent: Sunday, June 04, 2000 10:41 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: NEED HELP WITH EDS ON A JEOL 100S
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListser} ver/FAQ.html
}
}
} --------------------------------------------------------------
} ---------.
}
}
} A fellow colleague has bought a JEOL 100s and wants and
} needs EDS X-ray
} Analysis. He's mounted his horizintal EDS detector , but can
} not get sample
} X-rays from the speciman. Contacting JEOL he found the
} problem to be a tilt
} problem. The 10 degree tilt from the normal SEG only tilts
} 10 degrees and a
} 30 to 60 degree tilt is necessary. At one time I was told
} that special
} sample holders and or double gap pole pieces were availabe.
} He wishs to find
} any one with a 100s for parts needed or information which
} would allow X-ray
} analysis. Please reply to mgmanders-at-aol.com or the list server.
}
} Mike Manders
}
}
}



From daemon Wed Jun 07 20:58:24 2000



From: Rick Powell at Nanoprobes :      rpowell-at-nanoprobes.com
Date: Wed, 7 Jun 2000 21:49:08 -0500
Subject: TEM: Set-up, operating and facilities costs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello List Members:

We have the opportunity to acquire a TEM, but budget constraints mean that
have to consider the cost of acquiring and operating this instrument
carefully against alternatives. We plan to use it principally to study the
morphology, behavior and size distribution of colloidal gold particles and
other types of metal nanoparticles.

Can anyone give any information on what the maintenance, operating and
facilities requirements and costs would be for this instrument, or point me
to a good source? What renovations would be necessary to accommodate it -
darkroom, ancillary equipment, cooling, water and power supplies, darkroom
facilities? How much should we budget for supplies and consumables? Since
we have no-one currently trained to use it, how long would it take to
learn, and how much maintenance (time, supplies, contracts) would it
require?

Thanks in advance,

Rick Powell


**********************************************************************
* - PLEASE NOTE OUR NEW ADDRESS - EFFECTIVE FEBRUARY 15, 2000 - *
* *
* NANOPROBES, Incorporated | US Toll-free: (877) 447-6266 *
* 95 Horse Block Road | Tel: (919) 510-0590 *
* Yaphank, NY 11980-9710, | Fax: (919) 510-0590 *
* USA | rpowell-at-nanoprobes.com *
* *
* NOW EASY TO FIND ON THE WEB: http://www.nanoprobes.com *
**********************************************************************




From daemon Wed Jun 07 23:18:25 2000



From: Barbara Foster :      mme-at-map.com
Date: Wed, 07 Jun 2000 23:34:37 -0400
Subject: Re: UV optics

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Don,

Both Zeiss and Leica have long-standing reputations for quartz optics with
transmission into the UV (typically, down to about 220nm). You are going to
need more than just the objective; the binoc also needs to have a UV
transmitting prism. A tip: inquire about microscopes used for
microspectrophotometry or in semiconductor applications. From the
biological/biomed realm: microscopes used for Fura or Indo studies.

Try Tom Calahan at Zeiss (914-681-7733) and Jan Hinsch at Leica/Allentown
(201-236-5905).

Nikon has also been agressive in lens development, but I haven't had direct
experience with any UV optics. Call Stan Schwartz (516-547-8529). ... and
at Olympus: Reinhard Enders (516-844-5000).

Good hunting!
Barbara Foster
Microscopy/Microscopy Education
125 Paridon Street, Suitee 102
Springfield, MA 01118

PH: 413-746-6931 FX: 413-746-9311 email: mme-at-map.com

Customized, on-site short courses in all areas of microscopy, sample prep,
and image analysis.
"Why didn't they teach us that sooner?" Probably because no one called MME!






At 03:27 PM 6/6/00 -0400, donald j marshall wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Wed Jun 07 23:18:25 2000



From: Barbara Foster :      mme-at-map.com
Date: Thu, 08 Jun 2000 00:12:10 -0400
Subject: Re: digital OMs for an EM lab

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Edorado,


Suggest you buy a good upright stand which you can upgrade as your needs
develop. I strongly suggest a combination of both transmitted and
reflected light options.


Your feelings re: investing in the microscope vs. a high end camera at
this stage are well founded.


Re: contrast techniques... I think that you might find Hoffman Modulation
Contrast a better bet for what you are doing than Phase. Hoffman can be
used singly for looking at surfaces (ex: your coatings) or in combination
with polarized light. It is a good complement to DIC, which won't work if
your powders, etc. respond to Pol.


All of the "big 4" (Olympus, Nikon, Leica, and Zeiss) have good
equipment. The issues I would add to your shopping list might include
how comfortable you feel operating a specific microscope (like trying on
a coat or test-driving a new car) and how supportive your local dealer or
representative is.


Your comment re: EM was interesting.... I have been doing a lot of work
over the past 6 months with commercial companies who look at many of the
applications you cited. Universally, they were astounded at the
information they could get from the Light Microscope, quickly and with
minimal sample prep. I am currently on assignment teaching a group of
very competent EM people about Light Microscopy... and they are equally
amazed. All by themselves, they came to the conclusion that they should
take a look first with the Light Microscope and then, if necessary, go
the SEM. (I added that they should also take a quick look with the stereo
first).


By the way, you may be interesting in "Optimizing Light Microscopy", both
as a reference for your lab and a text for your students. Details are on
our website.


Happy shopping!

Barbara Foster

Microscopy/Microscopy Education

125 Paridon Street, Suitee 102

Springfield, MA 01118


PH: 413-746-6931 FX: 413-746-9311 email: mme-at-map.com

www.MME-Microscopy.com/education


Customized, on-site short courses in all areas of microscopy, sample
prep, and image analysis.

"Why didn't they teach us that sooner?" Probably because no one called
MME!



At 09:47 AM 6/7/00 +0200, Bemporad, Edoardo wrote:

} } } }

{excerpt}

{fontfamily} {param} Arial {/param} {smaller} I am going to buy one ore two OM
for our EM lab (XL30 and CM120), trying to convince myself that one brand
is better than the other (quality/price rate included in the
evaluation!).

{/smaller} {/fontfamily}

{fontfamily} {param} Arial {/param} {smaller} In our lab we do mainly
metallographic analysis, interface studies of wear resistant coatings,
and catalytic powders characterization, but I guess that the OM will be
used for a wider range of investigation (W/O and O/W emulsions, asbestos,
..) and for didactical scopes too.

{/smaller} {/fontfamily}

{fontfamily} {param} Arial {/param} {smaller} So EPI and DIA illuminations,
BF, DF, NIC, phase contrast, pol, I don't think we will need
fluorescence; forgotten something?

{/smaller} {/fontfamily}

{fontfamily} {param} Arial {/param} {smaller} What about an inverse
microscope? or always better two standard ones? {/smaller} {/fontfamily}

{fontfamily} {param} Arial {/param} {smaller} I have about 35K$ budget to make
everybody happy (research group and students), and my position is to
prefer spending on the microscope (or microscopes) rather than on a
high-level digital camera. I read some threads about it here and I think
something like a Nikon coolpix 990 (if it will works! :-) ) will be
enough. (other opinions or point of views?)

{/smaller} {/fontfamily}

{fontfamily} {param} Arial {/param} {smaller} I do not have a so deep
experience in OM but I was wandering if there are some key feature to
keep in mind for an equipment that will be used in a EM lab, considering
that where I will not able to go with the OM (depth of field, resolution)
I can use our EM!

{/smaller} {/fontfamily}

{fontfamily} {param} Arial {/param} {smaller} Anybody have suggestion (base
set, optional...)? {/smaller} {/fontfamily}

{fontfamily} {param} Arial {/param} {smaller} I will post a report with the
collected hints; Thank You . {/smaller} {/fontfamily}


{fontfamily} {param} Arial {/param} {smaller} Dr. Eng. Edoardo Bemporad, Ph.
D. {/smaller} {/fontfamily}

{fontfamily} {param} Arial {/param} {smaller} Assistant Professor of Materials
Science {/smaller} {/fontfamily}

{fontfamily} {param} Arial {/param} {smaller} University of Rome "Roma Tre"
(Italy) {/smaller} {/fontfamily}

{fontfamily} {param} Arial {/param} {smaller} Dipartimento di Ingegneria
Meccanica e Industriale {/smaller} {/fontfamily}

{fontfamily} {param} Arial {/param} {smaller} (Department of Mechanical and
Industrial Engineering) {/smaller} {/fontfamily}

{fontfamily} {param} Arial {/param} {smaller} Via Vasca Navale 79 - 00146
Rome, Italy {/smaller} {/fontfamily}

{fontfamily} {param} Arial {/param} {smaller} Tel:
+39 {/smaller} {/fontfamily} {fontfamily} {param} Arial {/param} {smaller} 06
5517.3293 {/smaller} {/fontfamily}

{fontfamily} {param} Arial {/param} {smaller} Fax:
+39 {/smaller} {/fontfamily} {fontfamily} {param} Arial {/param} {smaller} 06
5517.3256 {/smaller} {/fontfamily}

{fontfamily} {param} Arial {/param} {smaller} LIME Lab Tel:
+39 {/smaller} {/fontfamily} {fontfamily} {param} Arial {/param} {smaller} 06
5517.3200 {/smaller} {/fontfamily}

{fontfamily} {param} Arial {/param} {smaller} LIME Web Site:
http//materials.dimi.uniroma3.it/lime {/smaller} {/fontfamily}

{fontfamily} {param} Arial {/param} {smaller} E-Mail:bemporad-at-uniroma3.it
{/smaller} {/fontfamily}


{/excerpt} { { { { { { { {




{/x-rich}



From daemon Wed Jun 07 23:18:26 2000



From: Barbara Foster :      mme-at-map.com
Date: Wed, 07 Jun 2000 23:47:15 -0400
Subject: Re: Help? Cleaning Optical Lenses

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Gina,

Most of the lenses should come off, either directly or with the help of a
small Allen wrench.

My favorite approach to lens cleaning: "Puff, Huff, and Swirl"....
1. Puff off any debris or rough material which might scratch the lens using
either a puffer (available from photographic supply houses) or just
"puffing" the dry air from your mouth with your cheeks (easier to demo than
explain).
2. Huff on the lens, using the warm, moist air from deep in your lungs, to
deposit a fog of moisture on the lens then
3. Quickly and gently, remove the fog with a clean Q-tip (100% cotton ) or
lens tissue (NOT Kim-Wipe!), starting at the middle of the lens and
continuing in a spiral to the outer edge. Do not use the same area on the
swab or tissue more than once; they will collect debris which can scratch
the delicate coating.
4. If the dirt is persistent (ex: mascara, fingerprint, oily residue), dip
the tip of a cotton swab in a good lens cleaning solution (also available
at photo supply houses), shake off any excess, then repeat the "swirl" step.

I recently had a client who had a neat moist towelette made by Uvex. It
worked really well, even on oil. I will have to find the contact info, so
if you are interested, please email me off line.

Best regards,
Barbara Foster
Microscopy/Microscopy Education
125 Paridon Street, Suitee 102
Springfield, MA 01118

PH: 413-746-6931 FX: 413-746-9311 email: mme-at-map.com

Customized, on-site short courses in all areas of microscopy, sample prep,
and image analysis.
"Why didn't they teach us that sooner?" Probably because no one called MME!


At 11:43 PM 6/6/00 -0500, wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Thu Jun 08 08:30:15 2000



From: Ron Doole :      ron.doole-at-materials.oxford.ac.uk
Date: Thu, 8 Jun 2000 08:37:05 +0100 (BST)
Subject: Re: JEOL 2010 LaB6 Question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hi Kim,

The real question is why should you increase the LaB6 heating current
slowly? As I understand it there are two reasons. Firstly if the tip is
new, the gun has been serviced or the gun has not been run at 200kV for
some time it is important to run up slowly to ensure that you do not get
any outgassing from the tip or other gun components that might cause a
flashover and possible damage. Secondly if the machine is regularly used
at 200kV and the gun is is good condition (normal case) then you want to
avoid any thermal shock to the LaB6 tip which could cause damage or
misalignment. The same applies to cooling the tip down after use.

In the first case I would certainly take several 10s of minutes while
carefully watching the vacuum gauge and the emission current meter (and HT
stability if you are connected to an oscilloscope). Exact time would
depend on the condition but maybe 30 minutes to heat a new tip and 2
minutes for a normal tip in good condition. I would take a minute to cool
down a tip.

In both cases the greatest heating effect is a square of the control
position (power is proportional to V^2 or I^2) and this should be taken
into acount. I heat to about 3.5 in 25 to 30 seconds then decrease my
heating speed as I approach the stop (set at 5 in my case, bias 5.5). The
particular setting will depend on the type of LaB6 tip used, the wenhelt
to tip distance and how you run the emission. We use 5uA emission with the
tip slightly undersaturated as it seems to give good results and
reasonable long life at the high mags that we use. For the smallest probes
we may desaturate further to reduce the probe size.

I am aware from visitors that we have from other sites that this is
quicker than many people but it seems impractical to spend 30 minutes to
run up the tip every time you want to change a specimen when we want to
look at several in a session. Our tip life des not seem to be any worse
than those who take much longer.

Regards,
Ron



} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Question:
} What is the proper procedure for increasing the filament current on a 2010
} with a LaB6?
} How fast can you go up?
} Should the current be increased at a linear rate or should the rate be
} tapered off as you reach saturation?
} The manual suggests 30 sec/graduation while the service techs suggest a
} much slower rate.
}
} Thanks,
} Kim
} **************************************************************
} Kim W. Pierson, Ph.D.
} Dept of Physics & Astronmoy
} University of Wisconsin-Eau Claire
} (715) 836-5009
} FAX 836-3955
}
}
}

===========================================================================
Mr. Ron Doole e-mail ron.doole-at-materials.ox.ac.uk
Department of Materials, phone +44 (0) 1865 273701
University of Oxford, fax +44 (0) 1865 283333
Parks Road.
Oxford. OX1 3PH. UK.
============================================================================



From daemon Thu Jun 08 08:30:15 2000



From: jim :      jim-at-proscitech.com.au
Date: Thu, 8 Jun 2000 11:09:37 +1000
Subject: RE: plant vesicles

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


LR White won't look as good as Spurr's, but membrane whorls are indicative of
insufficient fixation. Os is the better lipid (therefore membrane) fixative,
since you cannot use Os, you may need to increase the time and or concentration
of GA.
Cheers
Jim Darley

ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Thursday, June 08, 2000 1:51 AM, Mark West
[SMTP:mwest-at-ifcsun1.ifisiol.unam.mx] wrote:

}
} Hi,
}
} I'm trying to do immunogold studies on a membrane protein in a preparation
} of plant vesicles (from minus 80oC storage). I did an Epon embedding with
} standard procedure (glut and osmium) to check the ultrastructure and got
} decent-looking vesicles, but side by side an LR White prep (0.5% glut, 2%
} paraformaldehyde, without osmium) looks like a disaster, with granular
} material, vague membranish-like structures and whorls of membranes. Does
} anyone have any tips for LR White preps of vesicles, or any other ideas
} for embedding (resins, fixing, dehydration, embedding) to favour the
} immunogold process.
}
} Thanks,
}
} Mark
}
}
}
}
}
}
} ********************************************
} Mark West,
} Unidad de Microscopia Electronica,
} (Electron Microscopy Unit)
} Instituto de Fisiologia Celular,
} Universidad Nacional Autonoma de Mexico,
} 04510 Mexico D.F.
}
} tel (unidad/lab) *(525) 622 5610*
} (casa/home) (525) 619 3020
} Fax (525) 616 2282
} ********************************************
}



From daemon Thu Jun 08 08:30:21 2000



From: Per Hšrstedt :      per.horstedt-at-pathol.umu.se
Date: Thu, 08 Jun 2000 13:44:10 +0200
Subject: Re: Still alive?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Kris,
same thing happened to me, but re-subscribing took care of the problem.
Mailed to listserver, but no respons at all.


} Dear Microscopists,
} I'm just curious if something is wrong on my end or I'm unsuscribed against
} my will. I did not receive any posting during the past two-three weeks, and
} this is impossible in view of previously received 10-20 postings/day.
} Is there any explanation?
} Kris
} Dr. Kristof Kovacs
} Associate Professor
} President, Hungarian Society for Microscopy
} Phone: +36-(88)-421-684
} Fax: +36-(88)-328-643
} Mailing Address:
} University of Veszprem, P.O.Box 158, Veszprem
} H-8201 Hungary

Yours sincerely

Per Hšrstedt
Department of Medical Biosciences
Pathology
Unit for Electron Microscopy
University of UmeŒ
S-90187 UmeŒ
Sweden

phone int-46-90-7851541
fax int-46-90-7851215



From daemon Thu Jun 08 08:30:24 2000



From: prenitzer,brenda s :      prenitzer-at-lucent.com
Date: Thu, 8 Jun 2000 08:54:59 -0400
Subject: Re: TEM:beam damage vs voltage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Literature indicates that specimen damage due to heating decreases with
increasing accelerating voltage; however, there is a trade off because at
higher voltages materials fall victim to knock-on and sputtering damage. A
very good overview (with references) of specimen/beam interactions and beam
damage can be found on PP 49-55 "Transmission Electron Microscopy" by
Williams and Carter 1996 Plenum Press ISBN: 0-306-45342-X

Brenda I. Prenitzer, Ph.D.
Member of Technical Staff
Cirent Semiconductor (Lucent Technologies)
9333 S. John Young Parkway
6D-Lab
Orlando, FL 32819-8612

Phone: 407 371 7108
Fax: 407 371 6999

prenitzer-at-lucent.com
bsp101-at-worldnet.att.net


-----Original Message-----
} From: Larry Stoter [mailto:LPS-at-teknesis.demon.co.uk]
Sent: Wednesday, June 07, 2000 3:49 PM
To: Jonathan Barnard; microscopy-at-sparc5.microscopy.com


At 14:19 +0200 3/6/00, Jonathan Barnard wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

My understanding is as follows ....

There are different damage mechnisms at different voltages.

At lower voltages ( {200 kV) the main damage mechanism is, in effect,
specimen heating. And, the lower the voltage, the greater the
interaction (elastic scattering) with the specimen - so more damage
but greater contrast, the main reason users looking at resin sections
prefer lower voltages.

Increasing the voltage reduces specimen damage and tends to improve
resolution but at the expense of contrast. As the interest in
biological TEM moves to molecular biology, resolution is more
important. But, at high resolution, phase contrast becomes the
dominant factor in producing image constrast and phase constrast can
be significantly improved - without loss of resolution - by using
highly coherent FEG sources.

At voltages between 300kV and 400 kV, the energy of the electron
becomes sufficient to cause "direct" radiation damage - atoms are
displaced. At this point, specimen damage rates increase. The
particular voltage depends on the amtomic number of your specimen.

Damage rates can also be reduced by cooling. in particular, cooling a
specimen to liquid helium temperatures allows significantly greater
electron exposure before damage occurs.

So, for optimum imaging of molecular specimens you need 300 kV and a
FEG gun plus a liquid helium stage.

Regards,
--
Larry Stoter
JEOL (UK) Ltd
Silver Court, Watchmead, Welwyn Garden City, AL7 1LT, United Kingdom
tel: +44-(0)1707-377117, fax: +44-(0)1707-373254, e-mail:
larrys-at-jeoleuro.com



From daemon Thu Jun 08 08:47:07 2000



From: Peter Bond :      P.Bond-at-plymouth.ac.uk
Date: Thu, 8 Jun 2000 08:31:38 -0500
Subject: LRWhite flat embedding

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all

I remember a message some time ago, I think, suggesting a way to flat
embed in silicon molds using LRWhite resin. How the top was sealed
escapes me. Could anyone with a better filing system and/or memory
please send me a message or a reference on this.

Thanks.

Pete
--
Peter Bond
Plymouth Electron Microscopy Unit
University of Plymouth
Drake Circus
Plymouth
Devon
UK PL4 8AA
Tel/Fax: 01752 233092
email: pbond-at-plymouth.ac.uk




From daemon Thu Jun 08 08:47:08 2000



From: Ken Tiekotter :      tiekotte-at-up.edu
Date: Thu, 8 Jun 2000 08:35:01 -0500
Subject: SEM/TEM available...

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear All,

If you are looking for an inexpensive TEM and/or SEM, The University of
Portland has two such units you might be interested in.

1) Zeiss EM9s2, transmission electron microscopy, with additional high
voltage tank, replacement for vacuum tubes, fuses, new filaments, lots of
film cassettes, and film, original schematics, and operation manuals.

2) ETEC AutoScan, scanning electron microscope, with 60 and 90 degree
stages, several reconditioned column liner tubes, new apertures, new YAG
scintillator in original container, original schematics and operating
manuals, plus, video tapes on operation and use. This instrument was
original manufactured for INTEL and has a 50kV power supply.

Both units are operational!

Any interested parties or individuals can contact me off the server.

Ken Tiekotter, Adjunct Professor
The University of Portland
Department of Biology
5000 N. Willamette Blvd.
Portland, OR 97203 USA

(503) 413-5391





From daemon Thu Jun 08 08:52:47 2000



From: Siegel, Stephen :      ssiegel-at-EMSL.com
Date: Thu, 8 Jun 2000 09:56:58 -0400
Subject: TEM Analyst Needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


EMSL Analytical Inc. (Westmont, NJ) is interested in hiring a Transmission
Electron Microscopy Analyst (TEM) for its NJ Corporate Office. EMSL is the
world leader in asbestos analysis since 1981 with over 20 laboratory
locations worldwide.

Responsibilities include the preparation and analysis of air, bulk, and
water samples submitted by clients for asbestos content and other specialty
asbestos projects. College Degree in Materials Science or related field and
past experience with electron microscopy analysis preferred. . The ideal
candidate would be a detail orientated individual able to follow lab
protocols and procedures and able to work in a fast paced environment.

Full benefits package with salary commensurate with experience. Interested
individuals send resume and salary requirements to Stephen Siegel, CIH
(ssiegel-at-emsl.com) or fax to 856-858-4960.

Stephen Siegel
Stephen Siegel, CIH
Asbestos Lab Manager
EMSL Analytical, INC
107 Haddon Avenue
Westmont, NJ 08108
Phone:800-220-3675x1209 Fax:609-858-4960
email:ssiegel-at-emsl.com



From daemon Thu Jun 08 09:00:29 2000



From: garyliechty-at-att.net
Date: Thu, 8 Jun 2000 08:50:50 -0500
Subject: Re: Ceramic cross-section preparation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Ian,

Your main problem is with the use of SiC for grinding
and thinning.

Silicon carbide paper should not be used to prepare any
ceramic material, it is not hard enough to cut it
efficiently without damaging the substructure. SiC
becomes dull quite rapidly, with ceramic a dull abrasive
actually cracks the structure and causes pullout and
excessive chipping. You are compounding your problem
with the weight of the tool being used as well. While
the disc grinder is a well designed product and very
useful, it is quite heavy for samples of certain
thicknesses not to mention the additional pressure you
are applying is not helping any.

The solution is to use Diamond Lapping Film and apply
some Diamond Extender at the final stages of thinning to
reduce the surface tension between the water and the
sample. This reduces the sheer stress being applied to
the sample and will drastically reduce the possibility
of cracking.

Should you have interest, we offer a polishing machine
"The MultiPrep System" that allows you to prepare the
sample without the possibility of the tool being
misaligned or mishandled (tilted on edge) during prep.
Only the sample makes contact with the abrasive and the
plane of polish remains in tact throughout the
polishing/grinding process. A dial indicator (1 micron
increments) allows you to preset a known amount of
material to be removed and there is no operator
intervention until the sample is done. The amount of
force applied to the sample is constant regardless of
the operator.

If you wish to get more information about the MultiPrep
System, you may visit our website:
www.alliedhightech.com or contact me in person at 310-
635-2466.

Sincerely,

Gary Liechty
Manager, Technical Products
Allied High Tech Products, Inc.
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear all,
} I'm currently having a few problems preparing good cross-section specimens
} of ceramic thin films on LaAlO3 or NdGaO3 substrates.
}
} I can cut them okay, but thinning them often results in cracking as the
} thickness goes below 100 microns. I have a Gatan model 623 disk grinder and
} currently have access to SiC abrasive papers to grit sizes of 1500 or 2000,
} and also have the Gatan diamond polishing disks and specimen lapping kit.
}
} The problems are as follows:
} If we use the 1500 or 2000 grit SiC, we can often get the samples thin
} enough, but with poor surface finish which needs to be improved with
} dimpling (on both sides), we also risk cracking the sample as SiC papers
} often contain imperfections.
}
} Using the Gatan specimen lapping kit, I find that as the disk grinder is
} altered to grind off a further 10 microns, the edge of the sample often
} catches on the diamond disk and tears some of the diamond coating off,
} leaving a lump on the surface which then risks catching the specimen and
} cracking it.
}
} I have one possible alternative approach which I used in Sweden last year
} (with SiAlON ceramics) involving attaching the sample to a glass slide and
} polishing it using diamond spray on non-absorbent paper. I may consider
} trying this with these new materials.
}
} Other ideas would be welcome, however, as would suggestions of how to
} improve my present method. Please note that I do not have the budget to buy
} any expensive new polishing equipment (such as a tripod polisher, for
} instance), so the most welcome suggestions would be those that involve
} improvements to current techniques, use of different types of polishing
} consumables, etc..
}
} I hope to hear several suggestions, both from users and from the companies
} who producing polishing and lapping equipment. Why not post them with the
} list so we can all benefit from the sharing of experience?
}
} Best wishes
}
} =====
} Ian MacLaren
} Beijing Laboratory of Electron Microscopy
} Chinese Academy of Sciences, P.O. Box 2724
} 100080 Beijing
} China
} General Email: ian.maclaren-at-physics.org
} Work (esp. large attachments): maclaren-at-image.blem.ac.cn
}
} ____________________________________________________________
} Do You Yahoo!?
} Get your free -at-yahoo.co.uk address at http://mail.yahoo.co.uk
} or your free -at-yahoo.ie address at http://mail.yahoo.ie
}




From daemon Thu Jun 08 18:12:44 2000



From: Arey, Bruce W :      bruce.arey-at-pnl.gov
Date: Thu, 08 Jun 2000 07:24:57 -0700
Subject: JEOL 840 SEM Vacuum Problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have JEOL 840 SEM that has develop a leak at the specimen chamber door
interface and will not hold a vacuum. We have changed the o-ring around the
door, look for nicks, scratches any other damage around the door interface, none
found. We have also check the roughing valve (V4, LV3 and the pressure relief
valve) for leaks. Pressure holds in the gun area. The large door is only opened
when we have to replace or add a new attachment to the chamber and thats about
once a year. We have a vacuum specimen exchange port to enter and retrieve
samples. We have also check that seal and it also checks out OK.
We have had 2 JEOL engineers take a look at the system and at this point no luck
finding the cause or solution. You can respond to me off-line at
bruce.arey-at-pnl.gov
Thanks

Bruce W. Arey

PNNL-Battelle
Associate Scientist
Microstructural Characterization
bruce.arey-at-pnl.gov
509-376-3363
fax 509-376-6308





From daemon Thu Jun 08 18:12:44 2000



From: Yves Giroux :      ygiroux-at-istar.ca
Date: Thu, 08 Jun 2000 10:34:27 -0400
Subject: PGT parts for anyone

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Hi:

I am about to tear the guts out of an old PGT System4 console. I need some
of the electronics, but will toss the rest unless someone would like it.

Jonathan Krupp
Microscopy & Imaging Lab
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu










From daemon Thu Jun 08 18:12:46 2000



From: Bruce Brinson :      brinson-at-cnst.rice.edu
Date: Thu, 08 Jun 2000 09:47:26 -0500
Subject: Re: JEOL 2010 LaB6 Question

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hello Kim, Jordi & interested parties,
We have a filament ramp circuit on our LaB6 2010 that controls the filament
current. It is an accessory offered by Jeol. Jeol has set it to take about 8
minutes to bring the filament to max current. The max. current is still
controlled by the (preset) filament current knob on L1. Once the filament is
hot, when we change samples or other wise have the filament current off for
{5mi. we use the "quick timer" feature of this circuit which brings the filament
up in ~90 seconds.
The max. filament current is set to run just under saturation (so you can
just barely see the X when the beam is converged). The bias control is used to
set beam current to 10 uA. The setting changes with KV & filament wear. The
initial bias setting is a function of the physical position of the filament
relative to the Wehnelt cap. It changes every time the filament or cap are
serviced. As the tip wears the difference in bias settings say between 100 &
200 KV increases & both move up.
We are a multiple user facility. Our filament sees a of cycles & we always
have novice users. Filament life times are 400-1000 hours. This tends to track
with the novice user density.
As far as the drift Jordi mentioned. This is a non issue here & may be in
the filament design. We use the Gimble Phillips 60-06. I do a lot of carbon
work & can pretty much nail 3.4A when I get a beam. Yes the images are sharper
after we have been running a bit.

Bruce Brinson
Rice U.

usual disclaimer... no financial interest in companies mentioned.

Marti, Jordi wrote:

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}
} Kim:
}
} We have a 2010 and our procedure is to bring the filament dial (labeled
} off-10) to position #3. Then we go for coffee. After 20 min. or so we raise
} it to # 4, after a couple of minutes to #5 etc. until we get to the stop (
} in our case we have it at about 7 1/2). Even at this point we have filament
} drift for quite some time, so , we are not ready to take pictures for
} another 1/2 hr or so. By the way, we have the bias settings at 7 and 7 which
} is high. I would be interested in knowing what your settings are.
}
} I hope this helps
}
} Jordi Marti
}



From daemon Thu Jun 08 18:12:59 2000



From: John J. Bozzola :      bozzola-at-siu.edu
Date: Thu, 8 Jun 2000 11:13:17 -0500
Subject: Stereometry by computer

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A user of our facility is interested in making height measurements
from specimens viewed in the SEM. I am aware of the conventional way
of doing this: stereo pairs and optical viewer (with stereometer
parallax corrections). Is there a more modern (computerized) way of
doing this, say with anaglyphs?

Thank you.

John B.


####################################################################
John J. Bozzola, Ph.D., Director
Micro-Imaging and Analysis Center
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
####################################################################



From daemon Thu Jun 08 18:12:59 2000



From: Al :      alchazz-at-ix.netcom.com
Date: Thu, 8 Jun 2000 07:59:26 -0500
Subject: Re: SEM: agency wants SEM images?

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I work for an analytical lab, Analytical Answers, Inc., located in Woburn, MA.

We charge $200/hr for SEM imaging, high resolution SEM imaging is more. The
customers are free to do what they want with the SEM images.

They paid for them, they own them!

Al Jaszek




-------------------------------------
*Causa latet, eventus est notissimus*
-------------------------------------




From daemon Thu Jun 08 18:13:06 2000



From: DrJohnRuss-at-aol.com
Date: Thu, 8 Jun 2000 13:40:05 EDT
Subject: Re: Stereometry by computer

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In a message dated 6/8/00 12:38:48 PM, bozzola-at-siu.edu writes:

} A user of our facility is interested in making height measurements
} from specimens viewed in the SEM. I am aware of the conventional way
} of doing this: stereo pairs and optical viewer (with stereometer
} parallax corrections). Is there a more modern (computerized) way of
} doing this, say with anaglyphs?


There was a thread recently on measurement of stereo pairs by
computer-matching of points. Several software packages (one of the is Fovea
Pro - http://members.aol.com/FoveaPro) were mentioned as having this
capability. Whether or not the two images are put together as an anaglyph is
unimportant.



From daemon Thu Jun 08 18:13:09 2000



From: David Bentley :      dlb-at-u.arizona.edu
Date: Thu, 08 Jun 2000 11:39:28 -0700
Subject: Re: Help? Cleaning Optical Lenses

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Just a couple of additional comments to Barbara's procedure.

A Zeiss axiom: If the lens is not dirty, then cleaning it will never
improve it, it only risks damaging it. Do the least amount possible to
return the surface to clean. If the surface is only dusty, & puffing it
removes the problem, stop there. I look at the lens being cleaned, using
the ocular turned upside down as a loupe, at each step of the process.
When it's clean, you're done. Usually only external surfaces are
contaminated and need frequent or extensive cleaning. Internal lenses, a
dusting often suffices.

In dealing with a dissecting microscope with a zoom magnification
capability as opposed to one with click stops or a fixed magnification, be
very careful about disturbing the positional settings of the internal
lenses. Any changes to these settings will alter magnification for the
each of the two eyes and make an image that can't be justified in the
brain. This will require a service engineer to remedy. For heavily filmed
lenses, use short or broken cotton tipped applicator and get in the best
you can. Varying the zoom control will move the lenses up and down and
maybe give you enough room to work.

As Barbara wrote, removing loose dust is essential to preserving lens
quality. Lenses are coated with various coatings and these are easily
damaged. Puffers from camera stores are good. The red bulb ear syringe
for babies is excellent and usually readily available. I do use dusters
and compressed air although both are frowned upon, by some, as possibly
damaging lens coatings. If you use a duster, don't shake or tip it while
dusting the lens. This will expel liquid from the can and contaminate the
lens worse.

A good lens cleaner that is easy to get is Sparkle Glass Cleaner available
from Ace hardware, and grocery stores (No financial interests). Never use
Windex. It contains oils that will coat the lens.

As much as possible use the cotton tipped applicators rather than lens
tissues. Unless you wear latex or polyethylene gloves, finger oils will
get onto the tissue and be transferred to the lens. Another reason to shy
away from the tissue is the tendency to scrub the lens. I agree with
Barbara, NEVER, NEVER Kimwipes, I've been told from many sources they
contain many silica strands, and will scratch the delicate optical
coatings. On expensive lenses, make a single pass, using minimal pressure
straight across the lens, with the applicator, rolling the stick between
your fingers to present a fresh cotton surface to the lens and picking up
and getting the dust away from the lens. Alternate between wet (either
with lens cleaner or condensed breath (open mouth, no spit please) and dry
cotton. (Yes, it is often necessary to use a swirling motion on oculars
because of the amount of oil contamination from the eye lashes.)

It is best not to try to clean filters, there are a few recommended
procedures but all potentially damage the very precise and thin coatings of
the filter compromising its performance. (If anyone disagrees or has a
favorite procedure for filter cleaning, contact me off list, I'd like to
hear about it.)

Use of solvents or disassembling objectives or multiple lens stacks is
best left to the service engineer. Lenses use a variety of air and cement
interfaces to achieve resolution and aberration correction. Altering these
by dissolving the cement will degrade the lens. Getting the small lenses
back in exactly the same position is also very, very difficult and not for
the faint hearted.

There is a microscope repair workshop in the initial planning stages for
the Long Beach MSA meeting next year. It will target K-12 school teachers,
but will hopefully address many levels. If members of the list will be at
the meeting and are interested in attending this workshop to: 1. learn
basic repairs for their microscopes. 2. get ideas for their outreach
program. or 3. assist with putting on the workshop. Please e-mail me
telling me of your interest or asking for more information.

If you have any questions feel free to contact me off line.



From daemon Thu Jun 08 18:13:10 2000



From: Soumitra Ghoshroy :      ghoshroy-at-nmsu.edu
Date: Thu, 8 Jun 2000 12:11:37 -0700
Subject: RE: plant vesicles

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Jim is absolutely right about LR white. But instead of increasing the GA I
will probably increase paraformaldehyde conc. to 4%.

Good luck,

Soumitra


} } Hi,
} }
} } I'm trying to do immunogold studies on a membrane protein in a preparation
} } of plant vesicles (from minus 80oC storage). I did an Epon embedding with
} } standard procedure (glut and osmium) to check the ultrastructure and got
} } decent-looking vesicles, but side by side an LR White prep (0.5% glut, 2%
} } paraformaldehyde, without osmium) looks like a disaster, with granular
} } material, vague membranish-like structures and whorls of membranes. Does
} } anyone have any tips for LR White preps of vesicles, or any other ideas
} } for embedding (resins, fixing, dehydration, embedding) to favour the
} } immunogold process.
} }
} } Thanks,
} }
} } Mark
} }
} }
} }
} }
} }
} }
} } ********************************************
} } Mark West,
} } Unidad de Microscopia Electronica,
} } (Electron Microscopy Unit)
} } Instituto de Fisiologia Celular,
} } Universidad Nacional Autonoma de Mexico,
} } 04510 Mexico D.F.
} }
} } tel (unidad/lab) *(525) 622 5610*
} } (casa/home) (525) 619 3020
} } Fax (525) 616 2282
} } ********************************************
} }




*****************************************************************
Soumitra Ghoshroy Ph.D.
Electron Microscopy Lab
Box 3EML
New Mexico State University
Las Cruces, NM 88003
Tel: 505-646-3600
Fax: 505-646-5665
e-mail: ghoshroy-at-nmsu.edu
http://confocal.nmsu.edu/eml




From daemon Thu Jun 08 18:13:11 2000



From: Tamara Howard :      howard-at-cshl.org
Date: Thu, 8 Jun 2000 15:21:15 -0400 (EDT)
Subject: Re: Help? Cleaning Optical Lenses

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On Thu, 8 Jun 2000, David Bentley wrote:

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} -----------------------------------------------------------------------.
text deleted
}
} It is best not to try to clean filters, there are a few recommended
} procedures but all potentially damage the very precise and thin coatings of
} the filter compromising its performance. (If anyone disagrees or has a
} favorite procedure for filter cleaning, contact me off list, I'd like to
} hear about it.)
}
Please respond on-line! I'm sure that I'm not the only other person who
would be interested in this!

Tamara Howard
CSHL




From daemon Thu Jun 08 18:13:17 2000



From: Ann-Fook Yang (Ann-Fook Yang) :      yanga-at-Em.agr.ca
Date: Thu, 08 Jun 2000 16:44:55 -0400
Subject: Re: plant vesicles

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Try treat the glut- & Os-fixed sections you have with sodium metaperiodate before doing immunolabeling. It may work.

Reference:
M. Bendayan 1989. Protein A-gold and Protein G-gold postembedding immunoelectron microscopy. In Colloidal Gold: Principles, Methods and Applications Vol.1 Academic Press.



Ann Fook Yang
EM Unit,
Eastern Cereal and Oilseed Research Centre,
Rm 2091, K.W. Neatby Bldg.,
Central Experimental Farm,
Ottawa, Ontario, Canada K1A 0C6

Phone: 613-759-1638
Fax; 613-759-1701

} } } Mark West {mwest-at-ifcsun1.ifisiol.unam.mx} 06/07 11:50 AM } } }
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Hi,

I'm trying to do immunogold studies on a membrane protein in a preparation
of plant vesicles (from minus 80¼C storage). I did an Epon embedding with
standard procedure (glut and osmium) to check the ultrastructure and got
decent-looking vesicles, but side by side an LR White prep (0.5% glut, 2%
paraformaldehyde, without osmium) looks like a disaster, with granular
material, vague membranish-like structures and whorls of membranes. Does
anyone have any tips for LR White preps of vesicles, or any other ideas
for embedding (resins, fixing, dehydration, embedding) to favour the
immunogold process.

Thanks,

Mark






********************************************
Mark West,
Unidad de Microscopia Electronica,
(Electron Microscopy Unit)
Instituto de Fisiologia Celular,
Universidad Nacional Autonoma de Mexico,
04510 Mexico D.F.

tel (unidad/lab) *(525) 622 5610*
(casa/home) (525) 619 3020
Fax (525) 616 2282
********************************************





From daemon Thu Jun 08 18:13:18 2000



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Fri, 9 Jun 2000 08:48:46 GMT+1200
Subject: Re: JEOL 840 SEM Vacuum Problem

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Sorry, I don't have any useful suggestions, and for that reason, I'd
rather see responses posted to the list, if possible, as this gives
me a wonderful and otherwise unavailable opportunity to learn from
the experience of others.

cheers

rtch







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} -----------------------------------------------------------------------.
}
}
} We have JEOL 840 SEM that has develop a leak at the specimen chamber door
} interface and will not hold a vacuum. We have changed the o-ring around the
} door, look for nicks, scratches any other damage around the door interface, none
} found. We have also check the roughing valve (V4, LV3 and the pressure relief
} valve) for leaks. Pressure holds in the gun area. The large door is only opened
} when we have to replace or add a new attachment to the chamber and thats about
} once a year. We have a vacuum specimen exchange port to enter and retrieve
} samples. We have also check that seal and it also checks out OK.
} We have had 2 JEOL engineers take a look at the system and at this point no luck
} finding the cause or solution. You can respond to me off-line at
} bruce.arey-at-pnl.gov
} Thanks
}
} Bruce W. Arey
}
} PNNL-Battelle
} Associate Scientist
} Microstructural Characterization
} bruce.arey-at-pnl.gov
} 509-376-3363
} fax 509-376-6308
}
}
}
}
Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand



From daemon Thu Jun 08 18:13:19 2000



From: hpadams :      hpadams-at-bcm.tmc.edu
Date: Thu, 8 Jun 2000 15:59:48 -0500
Subject: RE: plant vesicles

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Mark, you didn't say how you post-stained after you immunolabeled. This step
can make all the difference in the world. For LRW, I prefer 3-4 secs in
saturated UA in 50% etoh, wash, followed by about 15secs in lead citrate. I
find a major difference (inferior) when I use aqueous UA. Also, going too long
in any of the solutions will give poor results.

} ===== Original Message From Mark West {mwest-at-ifcsun1.ifisiol.unam.mx} =====
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Hank Adams
Manager
Integrated Microscopy Core
Molecular and Cellular Biology
Baylor College of Medicine
Houston, Tx 77030



From daemon Thu Jun 08 18:13:29 2000



From: David Joswiak :      joswiak-at-orca.astro.washington.edu
Date: Thu, 8 Jun 2000 15:43:51 -0700 (PDT)
Subject: Re: Stereometry by computer

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John - I routinely make height measurements of 5 - 10 um interplanetary
dust particles using the objective focus knob on our JEOL 1200EX in STEM
mode. The procedure is simply to focus on the top of the particle and
note the number of steps (or count the number of clicks of the knob)
required when refocussing on the substrate next to the particle. By
calibrating the objective focus step size with a known standard, you can
routinely measure particle heights. In principle, a similar procedure
should be possible on an SEM, depending on how the objective focus is
configured. I don't think you can expect high accuracy with this method,
however, so it may not be applicable to your needs.

Dave Joswiak
Dept. of Astronomy
University of Washington
Seattle, WA 98195
joswiak-at-astro.washington.edu

On Thu, 8 Jun 2000, John J. Bozzola wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
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} -----------------------------------------------------------------------.
}
}
} A user of our facility is interested in making height measurements
} from specimens viewed in the SEM. I am aware of the conventional way
} of doing this: stereo pairs and optical viewer (with stereometer
} parallax corrections). Is there a more modern (computerized) way of
} doing this, say with anaglyphs?
}
} Thank you.
}
} John B.
}
}
} ####################################################################
} John J. Bozzola, Ph.D., Director
} Micro-Imaging and Analysis Center
} 750 Communications Drive - MC 4402
} Southern Illinois University
} Carbondale, IL 62901 U.S.A.
} Phone: 618-453-3730
} Fax: 618-453-2665
} Email: bozzola-at-siu.edu
} Web: http://www.siu.edu/departments/shops/cem.html
} ####################################################################
}
}



From daemon Thu Jun 08 18:13:29 2000



From: Robert Mixon :      mixonr-at-ohsu.edu
Date: Thu, 08 Jun 2000 15:59:22 -0700
Subject: RE: Cleaning optical lenses and filters.

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Tamara et al : One superior method for cleaning sensitive optical components such as filters and front surfaced telescope mirrors is as follows: 1) gently puff of extraneous dust. 2) gently float the surface with deionized water. 3) if necessary to remove dirt film use a q-tip soaked in a non-ionic surfactant like a 0.01% solution of triton-x 100 (IMPORTANT DO NOT RUB ON THE SURFACE WITH FORCE BUT SIMPLY LET THE WEIGHT OF THE WET Q-TIP(S) BE THE ONLY "DOWNWARD PRESSURE" ON THE SURFACE OF THE GLASS. Such gently techniques such as these should help mitigate damage.



From daemon Thu Jun 08 18:13:30 2000



From: Robert Mixon :      mixonr-at-ohsu.edu
Date: Thu, 08 Jun 2000 16:03:20 -0700
Subject: RE: Cleaning optical lenses and filters.

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Tamara et al : (excuse mispelling in first copy sent) One superior method for cleaning sensitive optical components such as filters and front surfaced telescope mirrors is as follows: 1) gently puff off extraneous dust. 2) gently float the surface with deionized water. 3) if necessary to remove dirt film use a q-tip soaked in a non-ionic surfactant like a 0.01% solution of triton-x 100 (IMPORTANT DO NOT RUB ON THE SURFACE WITH FORCE BUT SIMPLY LET THE WEIGHT OF THE WET Q-TIP(S) BE THE ONLY "DOWNWARD PRESSURE" ON THE SURFACE OF THE GLASS. Such gently techniques such as these should help mitigate damage.



From daemon Thu Jun 08 18:13:31 2000



From: Caroline Schooley :      schooley-at-mcn.org
Date: Thu, 8 Jun 2000 16:02:44 -0700
Subject: Re: LRWhite flat embedding

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} I remember a message some time ago, I think, suggesting a way to flat
} embed in silicon molds using LRWhite resin. How the top was sealed
} escapes me. Could anyone with a better filing system and/or memory
} please send me a message or a reference on this.

Peter Bond {P.Bond-at-plymouth.ac.uk}

} Pete -

If you have a silicon mold that isn't permeable to LR White, I'd like to
know who makes it! There is a teflon flat mold available from Ted Pella
that works well with LR White; it's their own product. It's sealed with
Thermanox coverslips, so you might try them with your mold.


Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html




From daemon Thu Jun 08 19:33:34 2000



From: Dopeyee-at-aol.com
Date: Thu, 8 Jun 2000 20:28:48 EDT
Subject: LEAK IN JEOL

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GET A HOLD OF A LEAK DETECTOR AND FIND THE TRUE AREA THAT IS LEAKING

I DO BELEIVE THE MANUFACTURER SHOULD BE OF MORE HELP

THEY HAVE TO FIX IT!!!!



From daemon Fri Jun 09 06:11:49 2000



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Thu, 08 Jun 2000 22:11:20 -0500
Subject: LR White flat embedding

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-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Peter Bond wrote:
==============================================================
I remember a message some time ago, I think, suggesting a way to flat embed
in silicon molds using LRWhite resin. How the top was sealed escapes me.
Could anyone with a better filing system and/or memory please send me a
message or a reference on this.
==============================================================
This might have been an old posting of mine. Since UV transparency is
usually required, we are talking about clear UV transparent silicone
embedding molds and we recommend a slight "overfilling" of the cavities.
Then when all cavities are filled, take another mold, just like the first
one, and place it on top, bottom side down, cavity side up. The capillary
action will result in a sealing out of any oxygen.

When the UV curing is complete, the top mold can be easily separated and
since the cavity side is still unused, no wear and tear has been put on it
in terms of taking away any of its lifetime.

Information about these transparent-to-UV embedding molds, and their use,
can be found on the SPI website given below.

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.spi.cc
########################
============================================




From daemon Fri Jun 09 06:12:09 2000



From: Oldrich Benada :      benada-at-biomed.cas.cz
Date: Fri, 9 Jun 2000 09:19:55 +0200
Subject: A hole in the EDS spectrum

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Dear colleagues,
We have a problem with spectra recording in our EDAX DX4 system
mounted on Philips CM12/STEM. There is a hole in the spectrum between
0.5keV and 3.5keV.
With service engineer, we have borrowed all the boards in electronics
and exchange our boards with the borrowed ones. The hole in the
spectrum remains. We have also dismounted detector from the EM column
and the Be window was checked for contamination. The window was clean
and intact.
Please, can anybody give us any hints how to solve our problem? Many
thanks in advance for any comment.
Oldrich Benada

Our system specification:
Philips CM12/STEM
EDAX DX4 system with 184 preamp. based on win3.11





+-----------------------------------+
Oldrich Benada
Acad. Sci. CR
Institute of Microbiology
Laboratory of electron microscopy
Videnska 1083
CZ - 142 20 Prague 4 - Krc
Czech Republic
+------------------------------------+
Phone: +420-2-4752399
Fax: +420-2-4715743
WEB:http://www.biomed.cas.cz/mbu/lem113/lem.htm



From daemon Fri Jun 09 06:12:09 2000



From: Ulrike Zeile :      ulrike.zeile-at-bal-tec.com
Date: Fri, 9 Jun 2000 09:44:26 +0200
Subject: AW: service

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Hello Mr.Patel,

Balzers Union renamed to BAL-TEC AG in 1992. But the product
line was maintained and new developments are carried out.
For additional information have a look at our website www.bal-tec.com.
There are several coating systems and freeze fracture systems of this older
types in use.

Our representative in USA / Canada:

Techotrade International
7 Perimeter Road
Manchester, NH 03103-3343
T: +1 603 622-5011

Our representative will contact you directly to assist with
further information.

-----UrsprŸngliche Nachricht-----
Von: Rajesh Patel [mailto:rpatel-at-UMDNJ.EDU]
Gesendet: Mittwoch, 31. Mai 2000 19:42
An: microscopy-at-sparc5.microscopy.com
Betreff: service


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We need service on our Balzer 500 freeze fractrure unit,
and was wondering who services them. Anyone we can contact?

Rajesh Patel
Robert Wood Johnson Medical School
Dept. of Pathology
Electron Microscopy Lab
675 Hoes Lane
Piscataway, NJ 08854

(732)235-4648
rpatel-at-umdnj.edu



From daemon Fri Jun 09 06:12:15 2000



From: Dr Malcolm Roberts :      malc-at-rock.ru.ac.za
Date: Fri, 09 Jun 2000 11:53:08 +0200
Subject: Carbonate standards - bizarre behaviour

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G'day Folks,
I acquired some carbonate standards a while ago from the Smithsonian
Insitute. Namely, Calcite, Siderite, Dolomite and Strontianite.
Interestingly, when admiring them with our ED system, there appeared an
anomalous peak around about where B is supposed to be. This is not a
detector artifact as it is specific only to these materials. I just
wondered if anyone else had noticed the same thing, or whether anyone
has any clues on why this should be occuring. The programme suggests
that there is about 50 wt % B in these things which seems unbelievable
considering the compositions supplied by Washington. Ideas?
Cheers,
Malc.

--
Dr MP Roberts Phone: [+27](0)46 603 8313
Dept of Geology Fax: [+27](0)46 622 9715
Rhodes University Cell: 083 4060 262 (usually off)
6140 Grahamstown e-mail: malc-at-rock.ru.ac.za
SOUTH AFRICA




From daemon Fri Jun 09 07:42:10 2000



From: HARRISm-at-esm-semi.co.uk
Date: Fri, 09 Jun 2000 10:12 +0000 (GMT)
Subject: RE: JEOL840 SEM VACUUM PROBLEMS

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It may not help but .....

With regard to the vacuum problems encountered by Bruce , without
being familiar with the instrument we encountered a ' similar '
problem and were misled into thinking a chamber door leak existed by
an over zealous leak detection unit .
The problem on our sem actually turned out to be a hairline crack in a
metal bellows attached to a valve that appeared OK . The vaccum would
not exceed a certain level in pumpdown in its final stages .

I believe these bellows were often a source of problems on older
instruments .

M.HARRIS harrism-at-esm-semi.co.uk
ESM LTD
South Wales , U.K .



From daemon Fri Jun 09 08:52:00 2000



From: Leona Cohen-Gould :      lcgould-at-mail.med.cornell.edu
Date: Fri, 9 Jun 2000 08:51:11 -0400
Subject: RMC microtomes

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Hi All,
I'm posting this for a friend who is not on-line.

Does anyone out there have a used RMC MT-7000 for sale? My friend just
moved to a lab that had an MT-5000 which she was told was operational. A
major exaggeration. She is an RMC loyalist, and desparately wants another,
but has a limited budget.

Please contact: Linda Burg Friedman at Columbia P & S at (212)305-9047

Thanks,
Lee

Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Confocal Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175




From daemon Fri Jun 09 08:52:00 2000



From: Steve Chapman :      protrain-at-emcourses.com
Date: Fri, 9 Jun 2000 14:34:56 +0100
Subject: Re: JEOL 840 SEM Vacuum Problem

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Hi

The specimen chamber is a vast area with many potential leak sources.

The door is the most likely if it is being opened on a regular basis,
however do not forget that the stage drives pass through the door and they
are being used all of the time.

If you are sure the door "O" ring is OK check the stage drive feed throughs
as they may be the source of your problem?

Steve Chapman
Senior Consultant Protrain
For consultancy and professional training in EM world wide
Tel 44+ 1280 814774 Fax 814007
www.emcourses.com



From daemon Fri Jun 09 09:01:58 2000



From: Leona Cohen-Gould :      lcgould-at-mail.med.cornell.edu
Date: Fri, 9 Jun 2000 09:05:24 -0400
Subject: Re: TEM: Set-up, operating and facilities costs

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Rick,
YOu don't mention make or modle of TEM, but i can tell you that my service
contract (2 preventive maintenance call, unlimited service calls, parts,
labor) costs around $15,500/year.
You will need a water supply for cooling and electrical work to bring in a
dedicated line. You will need a dakrroom with running water and a temp.
control valve to process the film. If you wish to make photographic prints
of your negatives, you will also need a point source enlarger (Durst was
the best, but they are hared to find, Omega used to make one, too) and
either pans (slow and painful) or a rapid processor.
Budgeting for supplies is a tough call...it depends on your usage.
If you find someone with an EM background to run it, its should't take them
long to learn the individual instrument. Training someone from ground zero
could take months to get dthe person on his/her way.
Ancillary euqipment: it depends on what you will be doing, biological or
materials, embedding/sectioning or particulates, negataive staining or
metal shadowing.

A really good source to check is Audrey Glauert's Practical Methods in
Electron Microscopy Vol. 4: Design of the Electron Microscope Laboratory,
by Ronald H. Alderson, North-Holland publishers, 1975.

If you wish to contact me off-line, I can go over my lab's operating budget
with you. I run a basic EM Core Facility here at (what used to be called)
Cornell Medical College.

Lee

Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Confocal Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175




From daemon Fri Jun 09 09:11:57 2000



From: Arey, Bruce W :      bruce.arey-at-pnl.gov
Date: Fri, 09 Jun 2000 07:05:10 -0700
Subject: RE: LEAK IN JEOL

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JEOL has been very helpful and they are somewhat limited on what they can do to
this instrument. The 840 has radioactive particles inside the chamber so we have
to be careful each time we open the system up plus there hands on is limited to
just oversight. I am in the process of trying to find a leak detector on site
that can be used on radioactive system. But we also cannot pump down the chamber
enough to use a leak detector. So we are in the process of making a plate that
will fit over the door. We have taken off all our accessories off the chamber
(EDS, OIM, BSE) and have pressurized the system and have found some evidence of
a leak on the left hand side on the door, we have changed the o-ring and still
the seal leaks on this side of the door. We have polished the door seal to make
sure there is no major scratches or marks. We are pretty confident that specimen
exchange port is OK we can pump this down and we see no signs of a leak. Why are
we focusing our attention on the large o-ring and chamber door. We can clean
the o-ring with alcohol (methanol or ethanol) and we can rough pump the chamber
out and put the chamber on the high vac system but after a period of time the
alcohol dries out and the system shuts down too a poor vacuum (overnight). Then
we try to rough pump the chamber and we cannot go more than 20-30 mamps
difference on the pirani gauge. We have done this several times with the same
results. We are going to try to pressurize the system with He and try He sniffer
to help us maybe pin point the leak. Thanks to all who have responded with
suggestion on finding the leak and if we are successful in finding the leak I
will post the finding on the server. Again JEOL has been very responsive in
trying to find the leak and they are somewhat limited on this instrument do to
its environment. Any other suggestion are welcomed.


Bruce W. Arey

PNNL-Battelle
Associate Scientist
Microstructural Characterization
bruce.arey-at-pnl.gov
509-376-3363
fax 509-376-6308


----------
From: "Dopeyee-at-aol.com"-at-sparc5.microscopy.com
Sent: Thursday, June 8, 2000 4:28 PM
To: Microscopy-at-sparc5.microscopy.com
Subject: LEAK IN JEOL

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GET A HOLD OF A LEAK DETECTOR AND FIND THE TRUE AREA THAT IS LEAKING

I DO BELEIVE THE MANUFACTURER SHOULD BE OF MORE HELP

THEY HAVE TO FIX IT!!!!



From daemon Fri Jun 09 09:11:57 2000



From: Huggins, Bradley J :      HUGGINBJ-at-bp.com
Date: Fri, 9 Jun 2000 09:05:55 -0500
Subject: RE: JEOL 840 SEM Vacuum Problem

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I recall many years ago we had a similar vacuum leak on our 840A that was
related to the specimen exchange port mechanism. The moving parts in the
sliding door mechanism had become slightly miss-aligned. We ended up
disassembling that mechanism, installing new o-rings and lubricating with
Apiezon L. Problem gone.
Hope your leak is that easy!
Good Luck
Brad Huggins
BP Amoco, Naperville

} ----------
} From: Arey, Bruce W[SMTP:bruce.arey-at-pnl.gov]
} Sent: Thursday, June 08, 2000 9:24 AM
} To: 'Microscopy-at-msa.microscopy.com'
} Subject: JEOL 840 SEM Vacuum Problem
}
} ------------------------------------------------------------------------
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} -----------------------------------------------------------------------.
}
}
} We have JEOL 840 SEM that has develop a leak at the specimen chamber door
} interface and will not hold a vacuum. We have changed the o-ring around
} the
} door, look for nicks, scratches any other damage around the door
} interface, none
} found. We have also check the roughing valve (V4, LV3 and the pressure
} relief
} valve) for leaks. Pressure holds in the gun area. The large door is only
} opened
} when we have to replace or add a new attachment to the chamber and thats
} about
} once a year. We have a vacuum specimen exchange port to enter and retrieve
} samples. We have also check that seal and it also checks out OK.
} We have had 2 JEOL engineers take a look at the system and at this point
} no luck
} finding the cause or solution. You can respond to me off-line at
} bruce.arey-at-pnl.gov
} Thanks
}
} Bruce W. Arey
}
} PNNL-Battelle
} Associate Scientist
} Microstructural Characterization
} bruce.arey-at-pnl.gov
} 509-376-3363
} fax 509-376-6308
}
}
}



From daemon Fri Jun 09 17:35:54 2000



From: milesd-at-us.ibm.com
Date: Fri, 9 Jun 2000 10:24:19 -0400
Subject: RE: Cleaning optical lenses and filters.

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I have always understood that deionized water would etch metal,
that being the reason for PVC pipe being used to deliver it. If this
is true, wouldn't that damage the first surface mirrors?

Darrell



From daemon Fri Jun 09 17:35:55 2000



From: ComCryLab1-at-aol.com
Date: Fri, 9 Jun 2000 10:31:59 EDT
Subject: LM: alignment

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David,
We have a B&L StereoZoom (0.7X - 3X) microscope which we
intend to donate to the local school system
(Computer VoTech Dept) for use in examining the circuit patterns
on silicon wafers that I have donated to them.

My problem is that the microscope is not functioning properly.
The two optical paths are not synchronized during zooming and
therefore the image tends to rotate or it falls out of focus. We have
tried to repair it in-house but we were unsuccessful. Knowing that
that we would create additional problems, we did not disturb the
lens or prism sub-assembly.

Is there a set of maintenance instructions or a tech manual available
to help us align this scope?

Thanks
Mike Urbanik
Commercial Crystal Labs
Naples, FL
www.crystalguru.com



From daemon Fri Jun 09 17:35:57 2000



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Fri, 9 Jun 2000 08:46:11 -0600
Subject: Re: Stereometry by computer

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John,

you may want to try the method below if your samples show very large
structures. One of the nice features of an SEM is it's large depth of
focus, unfortunately in many cases this prevents you from using the
technique mentioned below. You can use a stereo technique to calculate a
surface profile (see for example the stereo module on our web site or
other stereo applications). Typical results from stereo images have a
height resolution of about 1/10 the of the lateral resolution (in other
words: if your lateral resolution is 1 micron between pixels, the height
resolution will be on the order of 10 microns). This can be improved by
sub-pixel interpolation but gives you an order of magnitude for the
resolution.

Michael

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: David Joswiak [mailto:joswiak-at-orca.astro.washington.edu]
Sent: Thursday, June 08, 2000 4:44 PM
To: John J. Bozzola
Cc: Microscopy-at-sparc5.microscopy.com


John - I routinely make height measurements of 5 - 10 um interplanetary
dust particles using the objective focus knob on our JEOL 1200EX in STEM
mode. The procedure is simply to focus on the top of the particle and
note the number of steps (or count the number of clicks of the knob)
required when refocussing on the substrate next to the particle. By
calibrating the objective focus step size with a known standard, you can
routinely measure particle heights. In principle, a similar procedure
should be possible on an SEM, depending on how the objective focus is
configured. I don't think you can expect high accuracy with this
method,
however, so it may not be applicable to your needs.

Dave Joswiak
Dept. of Astronomy
University of Washington
Seattle, WA 98195
joswiak-at-astro.washington.edu

On Thu, 8 Jun 2000, John J. Bozzola wrote:

}
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}
}
} A user of our facility is interested in making height measurements
} from specimens viewed in the SEM. I am aware of the conventional way
} of doing this: stereo pairs and optical viewer (with stereometer
} parallax corrections). Is there a more modern (computerized) way of
} doing this, say with anaglyphs?
}
} Thank you.
}
} John B.
}
}
} ####################################################################
} John J. Bozzola, Ph.D., Director
} Micro-Imaging and Analysis Center
} 750 Communications Drive - MC 4402
} Southern Illinois University
} Carbondale, IL 62901 U.S.A.
} Phone: 618-453-3730
} Fax: 618-453-2665
} Email: bozzola-at-siu.edu
} Web: http://www.siu.edu/departments/shops/cem.html
} ####################################################################
}
}



From daemon Fri Jun 09 17:36:06 2000



From: Tracey M. Pepper :      tpepper-at-iastate.edu
Date: Fri, 09 Jun 2000 12:29:09 -0500
Subject: LR White flat embedding

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This may sound like "bucket science" but we've used the disposable aluminum
weighing dishes to flat embed material. If you fill the bottom tray about
1/2 full and set another tray inside it, press gently until a little LR
white oozes up the sides, what remains in the middle will be protected from
the air and should polymerize nicely. (Maybe we've just been lucky!)
good luck!

Tracey Pepper
Supervisor
Bessey Microscopy Facility
Iowa State University
ph: 515-294-3872
fax: 515.294.1337



From daemon Fri Jun 09 17:36:07 2000



From: Jim McGee :      mcgee-at-geol.sc.edu
Date: Fri, 09 Jun 2000 13:53:37 -0400
Subject: Re: Carbonate standards - bizarre behaviour

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Malcolm:

Some Chlorine-L lines, as well as Sr lines have nearly identical
energy/wavelength as B Ka. I don't remember the chemistry of these
standards offhand, but I know some of them were Sr-bearing and some may also
have Cl. These are likely the peaks you are seeing at the low end.

Jim
--
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
James J. McGee (email: jmcgee-at-sc.edu)
Department of Geological Sciences
University of South Carolina
Columbia, SC 29208

Tel: 803-777-6300 Fax: 803-777-6610


Dr Malcolm Roberts wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} G'day Folks,
} I acquired some carbonate standards a while ago from the Smithsonian
} Insitute. Namely, Calcite, Siderite, Dolomite and Strontianite.
} Interestingly, when admiring them with our ED system, there appeared an
} anomalous peak around about where B is supposed to be. This is not a
} detector artifact as it is specific only to these materials. I just
} wondered if anyone else had noticed the same thing, or whether anyone
} has any clues on why this should be occuring. The programme suggests
} that there is about 50 wt % B in these things which seems unbelievable
} considering the compositions supplied by Washington. Ideas?
} Cheers,
} Malc.
}
} --
} Dr MP Roberts Phone: [+27](0)46 603 8313
} Dept of Geology Fax: [+27](0)46 622 9715
} Rhodes University Cell: 083 4060 262 (usually off)
} 6140 Grahamstown e-mail: malc-at-rock.ru.ac.za
} SOUTH AFRICA







From daemon Fri Jun 09 17:36:10 2000



From: bobrob-at-uswest.net
Date: Fri, 09 Jun 2000 11:49:20 -0700
Subject: SEM: JEOL 840 Vacuum Problem

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If the instrument has been physically moved recently, this can sometimes

hasten a crack in these stainless steel "flex" vacuum lines. I have
found
these to develop mostly at the end of the tube where it has been flared
for
the fitting connector. Check the roughing lines first.

Happy hunting...

Bob Roberts
EM Lab Services, Inc
2409 S. rural Rd Suite C
Tempe, Arizona 85282
480.967.3946



From daemon Fri Jun 09 17:36:10 2000



From: Huggins, Bradley J :      HUGGINBJ-at-bp.com
Date: Fri, 9 Jun 2000 12:18:33 -0500
Subject: RE: A hole in the EDS spectrum

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} Oldrich
Need a little more information.
Do you actually have spectral information in the spectrum at energies { 0.5,
or is there possibly, only noise in this low energy range? If the signal
present in your spectra at these very lowest energies is just noise-like
signal. Then it is possible that you have a severe alignment problem with
the EDS detector/specimen geometry. A combination of high noise (due to
vibration or other sources) and poor line of sight with the detector window
(resulting in detection of only the higher energy x-rays ) could give you
this "hole in the EDS spectrum" effect. A miss-aligned detector, and/or a
detector making contact with the internal parts of the microscope might
create this situation. Does the information in the low energy region
correlate with the specimen composition? Does the high energy signal
correlate with the specimen composition?

} ----------
} From: Oldrich Benada[SMTP:benada-at-biomed.cas.cz]
} Sent: Friday, June 09, 2000 2:19 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: A hole in the EDS spectrum
}
} ------------------------------------------------------------------------
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} -----------------------------------------------------------------------.
}
}
} Dear colleagues,
} We have a problem with spectra recording in our EDAX DX4 system
} mounted on Philips CM12/STEM. There is a hole in the spectrum between
} 0.5keV and 3.5keV.
} With service engineer, we have borrowed all the boards in electronics
} and exchange our boards with the borrowed ones. The hole in the
} spectrum remains. We have also dismounted detector from the EM column
} and the Be window was checked for contamination. The window was clean
} and intact.
} Please, can anybody give us any hints how to solve our problem? Many
} thanks in advance for any comment.
}
} Oldrich Benada
}
} Our system specification:
} Philips CM12/STEM
} EDAX DX4 system with 184 preamp. based on win3.11
}
}
}
}
}
} +-----------------------------------+
} Oldrich Benada
} Acad. Sci. CR
} Institute of Microbiology
} Laboratory of electron microscopy
} Videnska 1083
} CZ - 142 20 Prague 4 - Krc
} Czech Republic
} +------------------------------------+
} Phone: +420-2-4752399
} Fax: +420-2-4715743
} WEB:http://www.biomed.cas.cz/mbu/lem113/lem.htm
}



From daemon Fri Jun 09 17:36:14 2000



From: hard-at-acsu.buffalo.edu
Date: Fri, 09 Jun 2000 16:18:05 -0500
Subject: Course Announcement I

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Course Announcement

Title: Optical Microscopy and Imaging in the Biomedical Sciences

When: October 11 - October 19, 2000

Where: Marine Biology Laboratory, Woods Hole, MA, USA

Tuition: $2150 (Includes room and board, text, handouts, supplies)

Application Deadline: August 1, 2000

Admission application and information:
Carol Hamel, Admissions Coordinator
Marine Biological Laboratory
7 MBL Street
Woods Hole, MA 02543-1015
(508) 289-7401
Internet: admissions-at-mbl.edu
WWW: http://www.mbl.edu (Application forms available via Adobe Acrobat)

Course Director: Colin S. Izzard, State University of New York -at- Albany
Phone: [518] 442 - 4367
EMail: csizzard-at-csc.albany.edu

Course Description:

For Whom:
Designed primarily for research scientists, physicians, postdoctoral
trainees and advanced graduate students in animal, plant, medical and
material sciences. Non-biologists seeking a comprehensive introduction to
microscopy and video-imaging will benefit greatly from this course as well.
There are no specific prerequisites, but an understanding of the basic
principles of optics is desirable. Limited to 24 students.

The eight day course consists of lectures, laboratory demonstrations,
exercises and discussions that will enable the participant to obtain and
interpret microscope images of high quality, to perform quantitative optical

measurements, and to produce photographic and video records for
documentation
and analysis.

Topics to be covered include:
principles of microscope design and image formation
bright field, dark field, phase contrast, polarized light,
differential
interference contrast, interference reflection, and fluorescence
microscopy
confocal scanning microscopy, multiphoton excitation fluorescence
microscopy and image deconvolution
digital image restoration and 3-D reconstruction
video imaging, recording, enhancement, and intensification
analog and digital image processing and analysis
fluorescent probes and ratiometric-imaging
laser tweezers and laser scissors

Applications to live cells will be emphasized; other specimens will be
covered as well.

Students will have direct hands-on experience with state-of-the-art
microscopes, video cameras, recorders and image processing equipment
provided
by major optical and electronics companies. Instruction will be provided by
experienced staff from universities and industry.

Students are encouraged to bring their own biological (primary
cultures, cell lines, etc.) and material specimens and to discuss individual

research problems with the faculty.



From daemon Fri Jun 09 17:50:39 2000



From: David Cockayne :      david.cockayne-at-materials.oxford.ac.uk
Date: Fri, 9 Jun 2000 17:38:24 -0500
Subject: University of Oxford: Job Vacancies

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University of Oxford: Job Vacancies



DEPARTMENT OF MATERIALS



Postdoctoral Research Assistant - Electron Microscopy of Crystalline
Materials



Salary £16,286 - £24,479 p.a.

A three-year position is available in a research group being developed by
Professor David Cockayne FRS for the study of a range of materials using
electron diffraction, electron microscopy (EM) and modelling techniques.
Quantitative microscopy and materials modelling will refine structural
models of technologically important materials. Extensive expertise in EM,
diffraction, the preparation of crystalline materials for microscopy, and
strong computing skills, are essential. Expertise in microscopy of
semiconductors including quantum dots and computational techniques for
image simulation would be an advantage. The Department has outstanding EM
and modelling facilities. Please quote ref. DJ00/12.

Applications including a curriculum vitae, list of publications and the
names and addresses of three referees should be sent to The Administrator,
Department of Materials, University of Oxford, Parks Road, Oxford, OX1
3PH, from whom further particulars are available. The closing date for
applications is 14 June 2000.









From daemon Sat Jun 10 07:40:26 2000



From: jim :      jim-at-proscitech.com.au
Date: Sat, 10 Jun 2000 13:56:13 +1000
Subject: RE: LEAK IN JEOL

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I have found the use of clear silcone sealant (as used for windows etc) quite
effective to temporally fix or help to isolate large and medium vacuum leaks.
In many instance it can be applied externally over various fittings. Screw
holes are best covered first with a little tape, to facilitate the later the
removal of the dry silicone. Silicone outgases a fair bit for some hours, so
only major leaks can be determined immediately after applying the silicone.
The method seems crude but is effective to eliminate numerous fittings as the
source of a leak. I once operated a TEM for several months with a split
stainless bellow, patched with a smear of silicone sealant.
I don't suggest the use of that sealant on a permanent basis or in ion gutter
pumped parts of a column.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Saturday, June 10, 2000 12:05 AM, Arey, Bruce W [SMTP:bruce.arey-at-pnl.gov]
wrote:
}
}
} JEOL has been very helpful and they are somewhat limited on what they can do
} to
} this instrument. The 840 has radioactive particles inside the chamber so we
} have
} to be careful each time we open the system up plus there hands on is limited
} to
} just oversight. I am in the process of trying to find a leak detector on site
} that can be used on radioactive system. But we also cannot pump down the
} chamber
} enough to use a leak detector. So we are in the process of making a plate
} that
} will fit over the door. We have taken off all our accessories off the chamber
} (EDS, OIM, BSE) and have pressurized the system and have found some evidence
} of
} a leak on the left hand side on the door, we have changed the o-ring and
} still
} the seal leaks on this side of the door. We have polished the door seal to
} make
} sure there is no major scratches or marks. We are pretty confident that
} specimen
} exchange port is OK we can pump this down and we see no signs of a leak. Why
} are
} we focusing our attention on the large o-ring and chamber door. We can clean
} the o-ring with alcohol (methanol or ethanol) and we can rough pump the
} chamber
} out and put the chamber on the high vac system but after a period of time the
} alcohol dries out and the system shuts down too a poor vacuum (overnight).
} Then
} we try to rough pump the chamber and we cannot go more than 20-30 mamps
} difference on the pirani gauge. We have done this several times with the same
} results. We are going to try to pressurize the system with He and try He
} sniffer
} to help us maybe pin point the leak. Thanks to all who have responded with
} suggestion on finding the leak and if we are successful in finding the leak I
} will post the finding on the server. Again JEOL has been very responsive in
} trying to find the leak and they are somewhat limited on this instrument do
} to
} its environment. Any other suggestion are welcomed.
}
}
} Bruce W. Arey
}
} PNNL-Battelle
} Associate Scientist
} Microstructural Characterization
} bruce.arey-at-pnl.gov
} 509-376-3363
} fax 509-376-6308
}
}
} ----------
} From: "Dopeyee-at-aol.com"-at-sparc5.microscopy.com
} Sent: Thursday, June 8, 2000 4:28 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: LEAK IN JEOL
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} GET A HOLD OF A LEAK DETECTOR AND FIND THE TRUE AREA THAT IS LEAKING
}
} I DO BELEIVE THE MANUFACTURER SHOULD BE OF MORE HELP
}
} THEY HAVE TO FIX IT!!!!
}



From daemon Sat Jun 10 07:40:26 2000



From: jim :      jim-at-proscitech.com.au
Date: Sat, 10 Jun 2000 18:59:21 +1000
Subject: RE: Cleaning optical lenses and filters.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hmmm, and "I didn't even know that", but believed that deionised water had
metal ions removed from it and so in that respect its purer than 2x glass
distilled water. Then I was taught and believed! that metal pipes would
re-introduce metal ion back into the water! Being deionised the water has no
buffering capacity and therefore is neither acid nor alkaline, they told me and
I believed.
Education is expensive; must ask for my money back.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Saturday, June 10, 2000 12:24 AM, "milesd-at-us.ibm.com"-at-sparc5.microscopy.com
[SMTP:"milesd-at-us.ibm.com"-at-sparc5.microscopy.com] wrote:
}
}
} I have always understood that deionized water would etch metal,
} that being the reason for PVC pipe being used to deliver it. If this
} is true, wouldn't that damage the first surface mirrors?
}
} Darrell
}



From daemon Sat Jun 10 14:45:31 2000



From: milesd-at-us.ibm.com
Date: Sat, 10 Jun 2000 15:24:26 -0400
Subject: RE: Cleaning optical lenses and filters.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ahhh, but I have now been educated! Re-contamination of the
painstakingly purified water is the concern, and there is no
threat to the durability of the pipes. I had been mislead.

Darrell



From daemon Mon Jun 12 08:26:19 2000



From: Anaspec :      anaspec-at-icon.co.za
Date: Mon, 12 Jun 2000 07:57:51 +0200
Subject: RE: LEAK IN JEOL

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi
Vacuum leaks, what a pleasure!
Our tried and tested methods include using Petroleum Ether or Ethanol and
then Bostick Prestic or Blue tac as it is known in Australia.
Spraying alcohol around the suspected areas should show up the leak.
If you ant to try and stop a leak use the Prestic. Its that stuff you buy at
the stationary shop that is used to stick posters and pictures to a wall.
That is pliable, removable and really handy at sealing off a few suspect
areas.
We are currently working on a JEOL840 here with a leak. After many hours we
found that it was the gauge head that was leaking.

Good luck
Luc Harmsen
Anaspec, South Africa
Technical support on microscopy.
Tel + 27 (0) 11 476 3455
www.anaspec.co.za

ICEM 15 will be in Durban,
South Africa, 2002.
www.icem15.com

-----Original Message-----
} From: Arey, Bruce W [mailto:bruce.arey-at-pnl.gov]
Sent: Friday, June 09, 2000 4:05 PM
To: 'microscopy-at-msa.microscopy.com'


JEOL has been very helpful and they are somewhat limited on what they can do
to
this instrument. The 840 has radioactive particles inside the chamber so we
have
to be careful each time we open the system up plus there hands on is limited
to
just oversight. I am in the process of trying to find a leak detector on
site
that can be used on radioactive system. But we also cannot pump down the
chamber
enough to use a leak detector. So we are in the process of making a plate
that
will fit over the door. We have taken off all our accessories off the
chamber
(EDS, OIM, BSE) and have pressurized the system and have found some evidence
of
a leak on the left hand side on the door, we have changed the o-ring and
still
the seal leaks on this side of the door. We have polished the door seal to
make
sure there is no major scratches or marks. We are pretty confident that
specimen
exchange port is OK we can pump this down and we see no signs of a leak. Why
are
we focusing our attention on the large o-ring and chamber door. We can
clean
the o-ring with alcohol (methanol or ethanol) and we can rough pump the
chamber
out and put the chamber on the high vac system but after a period of time
the
alcohol dries out and the system shuts down too a poor vacuum (overnight).
Then
we try to rough pump the chamber and we cannot go more than 20-30 mamps
difference on the pirani gauge. We have done this several times with the
same
results. We are going to try to pressurize the system with He and try He
sniffer
to help us maybe pin point the leak. Thanks to all who have responded with
suggestion on finding the leak and if we are successful in finding the leak
I
will post the finding on the server. Again JEOL has been very responsive in
trying to find the leak and they are somewhat limited on this instrument do
to
its environment. Any other suggestion are welcomed.


Bruce W. Arey

PNNL-Battelle
Associate Scientist
Microstructural Characterization
bruce.arey-at-pnl.gov
509-376-3363
fax 509-376-6308


----------
From: "Dopeyee-at-aol.com"-at-sparc5.microscopy.com
Sent: Thursday, June 8, 2000 4:28 PM
To: Microscopy-at-sparc5.microscopy.com
Subject: LEAK IN JEOL

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America
To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
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-----------------------------------------------------------------------.


GET A HOLD OF A LEAK DETECTOR AND FIND THE TRUE AREA THAT IS LEAKING

I DO BELEIVE THE MANUFACTURER SHOULD BE OF MORE HELP

THEY HAVE TO FIX IT!!!!




From daemon Mon Jun 12 14:29:05 2000



From: Pauline C. Yu :      splene-at-pw.usda.gov
Date: Mon, 12 Jun 2000 08:29:05 -0700 (PDT)
Subject: SEM of liquid crystal

Contents Retrieved from Microscopy Listserver Archives
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Can anyone recommend a book or preferably a website with SEM images of
liquid crystal and or liquid crystal displays?

TX
Pauline Yu
pyu-at-pw.usda.gov
Microscopist Technician
USDA-ARS-WRRC



From daemon Mon Jun 12 16:51:40 2000



From: Norman Michaud :      Norman_Michaud-at-meei.harvard.edu
Date: Mon, 12 Jun 2000 16:09:40 -0400
Subject: TEM lab equipment available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We are consolidating two electron microscopy labs in the Boston area and
have some equipment which someone may want.
1) JEOL JEM 100CX electron microscope which has always been under service
contract and is still being used. It is about 20 years old and we would like
to see it in a new home rather than trashing it. You would need to have it
moved.
2)JEOL JEE 4C vacuum evaporator for Carbon. Still under vacuum and yours for
the taking.
3)Durst Laborator 138S floor model enlarger with many condensers and an Agfa
Rapidoprint DD6400 processor, both in very good shape and for sale.
Contact me directly with any questions.


Norman Michaud
Director, Morphology
Mass Eye and Ear Infirmary
Ophthalmology-5th flr.
243 Charles St, Boston, MA 02114
norman_michaud-at-MEEI.Harvard.edu
Tel:617-573-3316; Fax:617-573-4290



From daemon Mon Jun 12 17:59:09 2000



From: Hans Brinkies :      HBrinkies-at-groupwise.swin.edu.au
Date: Tue, 13 Jun 2000 08:48:34 +1000
Subject: Radioactice SEM?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Bruce W. Arey referred in his request to find a vacuum leak in his
JSM840 to 'radioactive particles' inside the chamber of his SEM. I
wonder if he could be more specific, because I feel if we start calling
a SEM as a 'radioactive system' many safety officers will have a field
day. I have been working with various SEMs since 1968 and never felt
that I might be bombarded by radioactive particles, even when I opened
the chamber (e.g. ETEC) of the SEM.



Hans Brinkies
Senior Lecturer
Swinburne, University of Technology
School of Engineering and Science
P.O.Box 218 - Hawthorn - Vic -3122 - Australia
Phone: +61 3 9214 8657
Fax: +61 3 9214 8264
Email: Hbrinkies-at-swin.edu.au



From daemon Mon Jun 12 23:41:44 2000



From: Gillian Bond :      gbond-at-nmt.edu
Date: Mon, 12 Jun 2000 17:43:23 -0600 (MDT)
Subject: Jet polishing of tungsten

Contents Retrieved from Microscopy Listserver Archives
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I have a student who has been trying to jet polish tungsten for TEM. She
has tried various concentrations of sodium hydroxide in water, as well as
40g trisodium phosphate/250ml water, and 55.8g magnesium perchlorate/250ml
methanol, at a range of voltages. We have a Fischione jet-polishing
unit. So far, none of the samples has been close to good. Can anyone
help us out here, with past experience or general suggestions?

Many thanks in advance,

Gill

Dr Gillian M. Bond
Department of Materials & Metallurgical Engineering
New Mexico Tech
Socorro, NM 87801



From daemon Mon Jun 12 23:41:46 2000



From: David Henriks :      Henriks-at-CompuServe.COM
Date: Mon, 12 Jun 2000 22:39:40 -0400
Subject: Jet polishing of tungsten

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Gill:

Certainly the best reference you can have for any jet polishing inquiry is
Bernie Kestel at Argonne National Laboratory. I will forward this to him
to see if he can add anything else. In digging through my extensive
"Bernie Archives" I did find a paper titled "A Jet Polishing Solution for
Silicon Germanium, Tantalum, Niobium and Tungsten-Rhenium" Ultramicrscopy 9
(1982) 379-384.

He was able to get a good polish under the following conditions:
Temperature: -50 C
Jet Height: 4.5mm (Single vertical jet system)
Pump setting: 6
Volts: 40
Current: 20mA

This was done using his BK-1 solution. BK-1 is prepared by mixing 500ml
methanol, 100ml butyl cellosolve, 90ml H2SO4 and 30ml HF.

He also has another paper MRS Volume 199 "Improved Methods and Novel
Techniques for Jet Electropolishing of TEM Foils" which lists a method for
electropolishing a 0.010" tungsten wire.

He was able to get a good polish under the following conditions:
Temperature: -50 C
Pump setting: 6
Volts: 120V

Using 6% HF, 12% sulphuric acid, 68% methanol, and 14% butyl cellosolve.

Of course these were done with a South Bay Vertical Jet system so you will
need to adjust the parameters for your system. Please get the reference
papers or contact me and I will send them to you. I have a long list of
Bernie's papers I could send you along with many other references on TEM
sample preparation that may be of interest. If you'd like to see more,
please contact me.

DISCLAIMER: South Bay Technology produces the Model 550 D Single Vertical
Jet Electropolisher as described above and, therefore, has a vested
interest in promoting its use.

Best regards-

David
Writing at 7:27:57 PM on 06/12/2000

***************************************************************************
************************

David Henriks
Vice President TEL: 800-728-2233
(toll free in the USA)
South Bay Technology, Inc. +1-949-492-2600
1120 Via Callejon FAX: +1-949-492-1499
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

***************************************************************************
************************

} } } } } Please visit us at http://www.southbaytech.com { { { { {

Manufacturers of precision sample preparation equipment and supplies for
metallography, crystallography and electron microscopy.

Message text written by Gillian Bond
}
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I have a student who has been trying to jet polish tungsten for TEM. She
has tried various concentrations of sodium hydroxide in water, as well as
40g trisodium phosphate/250ml water, and 55.8g magnesium perchlorate/250ml
methanol, at a range of voltages. We have a Fischione jet-polishing
unit. So far, none of the samples has been close to good. Can anyone
help us out here, with past experience or general suggestions?

Many thanks in advance,

Gill

Dr Gillian M. Bond
Department of Materials & Metallurgical Engineering
New Mexico Tech
Socorro, NM 87801
{



From daemon Mon Jun 12 23:41:48 2000



From: Tommy Davis :      barb29t-at-arabia.com
Date: Mon, 12 Jun 2000 22:42:05 -0500
Subject: Disaster Recovery Planning #5453

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Business continuity and disaster recovery planning has now
been made easier than ever with Version 7.3 of the
Disaster Recovery System (DRS) product. DRS provides a
plan for inaccessibility or inoperability (a disaster situation).

DRS is an industry standard software product, used by thousands
worldwide. DRS users are in large and small companies across
a wide variety of industries.

DRS conforms to federal regulations and meets insurance, auditing
and legal requirements. DRS runs under Windows, with stand-alone
and network versions available. DRS provides the most complete,
easy-to-use product available today.

To prove its value, a free trial is available. For more
information, visit our web site at
www.drsbytamp.com {http://www.drsbytamp.com} .
Dealer and distributor inquiries are welcomed

*****************************************************************
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section 301. Per Section 301, Paragraph (a)(2)(C) of S. 1618,
further transmissions to you by the sender of this email may be
stopped at no cost to you. This message is not intended for
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has been done to the best of our technical ability.If you are a
Washington, Virginia, or California resident please remove
yourself.
====================================================

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From daemon Mon Jun 12 23:41:48 2000



From: Tommy Davis :      barb29t-at-arabia.com
Date: Mon, 12 Jun 2000 22:42:05 -0500
Subject: Disaster Recovery Planning #5453

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Business continuity and disaster recovery planning has now
been made easier than ever with Version 7.3 of the
Disaster Recovery System (DRS) product. DRS provides a
plan for inaccessibility or inoperability (a disaster situation).

DRS is an industry standard software product, used by thousands
worldwide. DRS users are in large and small companies across
a wide variety of industries.

DRS conforms to federal regulations and meets insurance, auditing
and legal requirements. DRS runs under Windows, with stand-alone
and network versions available. DRS provides the most complete,
easy-to-use product available today.

To prove its value, a free trial is available. For more
information, visit our web site at
www.drsbytamp.com {http://www.drsbytamp.com} .
Dealer and distributor inquiries are welcomed

*****************************************************************
This message is sent in compliance of the new email bill
section 301. Per Section 301, Paragraph (a)(2)(C) of S. 1618,
further transmissions to you by the sender of this email may be
stopped at no cost to you. This message is not intended for
residents in the State of WA, CA & VA Screening of addresses
has been done to the best of our technical ability.If you are a
Washington, Virginia, or California resident please remove
yourself.
====================================================

///////////////////////////////////////////////////
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///////////////////////////////////////////////////





From daemon Tue Jun 13 07:32:46 2000



From: Per Hšrstedt :      per.horstedt-at-pathol.umu.se
Date: Tue, 13 Jun 2000 09:17:46 +0200
Subject: Zeiss EM 109 vacuum question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,
one of our TEM's , a Zeiss EM 109, is equipped with an ion getter hi-vac
pump. We are now planning to install a turbopump including all the piping
and valves that are necessary. The reason for this is that the high vacuum
is not good enough ( only in low E-6 area ) and the ion pump has to work
too hard and the life-time of the electrodes becomes very short. Also the
housing is clogged with trapped gas molecules and has to be regenerated far
too often.
Technically and electronically this exchange is fairly easily done, but my
question is if anyone has done it and if so, what's the experience?
Yours sincerely

Per Hšrstedt
Department of Medical Biosciences
Pathology
Unit for Electron Microscopy
University of UmeŒ
S-90187 UmeŒ
Sweden

phone int-46-90-7851541
fax int-46-90-7851215



From daemon Tue Jun 13 07:32:49 2000



From: Alexander Mironov :      mironov-at-cmns.mnegri.it
Date: Tue, 13 Jun 2000 11:31:30 +0200 (MET DST)
Subject: About monovalent Fab fragments conjugated with HRP

Contents Retrieved from Microscopy Listserver Archives
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Dear Friends,
For a long time we used Fab fragments-HRP from BioSys (France) for the
immuno EM labelling of proteins with subsequent preembedding. These
fragments were the best. However, recently the company cancelled its
activity and does not send any more these products.
Would you be so kind to tell me what has happened (if you know) and what
fragments have the same quality?

Sincerely yours, Alexander Mironov



From daemon Tue Jun 13 07:32:54 2000



From: White, Woody N :      Woody.N.White-at-mcdermott.com
Date: Tue, 13 Jun 2000 08:05:00 -0500
Subject: Re:Zeiss EM 109 vacuum question

Contents Retrieved from Microscopy Listserver Archives
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------------------------------------------------------------------------
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I changed my former SEM (Etec w/D.P)to a a Leybold mag-lev turbo a number of
years ago. The ball bearing pumps (of the day) caused too much vibration.
The
results were excellent! The Etec plumbing allowed the turbo to run
continuously
during vent cycles which was of great benefit.

Keep in mind that for any gas, turbos have a fixed compression ratio. Among
other things, the foreline pressure will have a direct influence on the
ultimate
vacuum.

The ion pumos are better than turbos for the cleanest, highest vacuum, but
you
have observed one weakness. They do not excel at pumping large volumes of
garbage-loaded gasses.

Good Luck,

Woody White
McDermott Technology

Hi,
one of our TEM's , a Zeiss EM 109, is equipped with an ion getter hi-vac
pump. We are now planning to install a turbopump including all the piping
SNIP



From daemon Tue Jun 13 07:32:55 2000



From: White, Woody N :      Woody.N.White-at-mcdermott.com
Date: Tue, 13 Jun 2000 08:10:00 -0500
Subject: Re:Radioactice SEM?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I believe The SEM to which Bruce refered was/is used to examine radioactive
materials. The "zoomies" are not from the SEM itself, but contamination
from
his specimens. My (former) Etec was in a similar condition. Over the
years, my
work mix resulted in a stage/chamber activitly level in the thousands of cpm
generated by radioactive products of nuclear fission. ...Kept the really
loose
stuff at a minimum, but certainly had to exercise the appropriate
precautions
when working on the system.

Woody White
McDermott Technology
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Bruce W. Arey referred in his request to find a vacuum leak in his
JSM840 to 'radioactive particles' inside the chamber of his SEM. I
wonder if he could be more specific, because I feel if we start calling
a SEM as a 'radioactive system' many safety officers will have a field
day. I have been working with various SEMs since 1968 and never felt
that I might be bombarded by radioactive particles, even when I opened
the chamber (e.g. ETEC) of the SEM.



Hans Brinkies
Senior Lecturer
Swinburne, University of Technology
School of Engineering and Science
P.O.Box 218 - Hawthorn - Vic -3122 - Australia
Phone: +61 3 9214 8657
Fax: +61 3 9214 8264
Email: Hbrinkies-at-swin.edu.au



From daemon Tue Jun 13 07:43:41 2000



From: Alexander Mironov :      mironov-at-cmns.mnegri.it
Date: Tue, 13 Jun 2000 07:33:27 -0500
Subject: TEM. About Fab-HRP

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Friends,
For a long time we used Fab fragments-HRP from BioSys (France) for the
immuno EM labelling of proteins with subsequent preembedding. These
fragments were the best. However, recently the company cancelled its
activity and does not send any more these products.
Would you be so kind to tell me what has happened (if you know) and what
fragments have the same quality?

Sincerely yours, Alexander Mironov




From daemon Tue Jun 13 08:12:54 2000



From: LAB. DE MICROSCOPIA ELECTRONICA - FI - UNER :      microsc-at-fi.uner.edu.ar
Date: Tue, 13 Jun 2000 09:50:51 +0000
Subject: looking for a critical point drier...

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The Laboratory of Electron Microscopy is looking for an used critical point
drier to obtain it in donation....
We can pay all the costs of shipping and handling
For any questions, please
contact to me....

best regards....
===================================================
Fernando D. Balducci
Laboratorio de Microscopia Electr—nica
Facultad de Ingenier’a - Bioingenieria
Universidad Nacional de Entre Rios
Argentina
e-mail: microsc-at-fi.uner.edu.ar
tel: 54 43 975100
fax: 54 43 975077


===================================================



From daemon Tue Jun 13 08:12:54 2000



From: Ron Doole :      ron.doole-at-materials.oxford.ac.uk
Date: Tue, 13 Jun 2000 14:08:45 +0100 (GMT Daylight Time)
Subject: Re: Zeiss EM 109 vacuum question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Per,

I have done this on a JEOL 4000 but not a Zeiss. I used a
Maglev TMP (Seiko Seiki) and an antivibration bellows to
couple the pump because I was afraid of vibration degrading
the 0.25nm resolution. I need not have worried, even with
the antivibration bellows shorted out I was OK.
Check that the TMP you choose does not give out any
magnetic fields when running.
If vibration is a problem then the Balzers (Pfieiffer)
antivibration bellows that has a large worm drive clip
around them can be tuned (by tightening the clip) to avoid
the instrument resonant frequency.

Good luck,
Ron

----------------------
Mr. R.C. Doole
Department of Materials,
University of Oxford.
Parks Road, Oxford. OX1 3PH. UK.
Phone +44 (0) 1865 273701
Fax +44 (0) 1865 283333
ron.doole-at-materials.ox.ac.uk

On Tue, 13 Jun 2000 09:17:46 +0200 Per
=?iso-8859-1?Q?H=F6rstedt?= {per.horstedt-at-pathol.umu.se}
wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi,
} one of our TEM's , a Zeiss EM 109, is equipped with an ion getter hi-vac
} pump. We are now planning to install a turbopump including all the piping
} and valves that are necessary. The reason for this is that the high vacuum
} is not good enough ( only in low E-6 area ) and the ion pump has to work
} too hard and the life-time of the electrodes becomes very short. Also the
} housing is clogged with trapped gas molecules and has to be regenerated far
} too often.
} Technically and electronically this exchange is fairly easily done, but my
} question is if anyone has done it and if so, what's the experience?
} Yours sincerely
}
} Per Hörstedt
} Department of Medical Biosciences
} Pathology
} Unit for Electron Microscopy
} University of Umeå
} S-90187 Umeå
} Sweden
}
} phone int-46-90-7851541
} fax int-46-90-7851215



From daemon Tue Jun 13 08:22:56 2000



From: Phoebe J Doss :      pjdoss-at-okstate.edu
Date: Tue, 13 Jun 2000 09:32:35 -0500
Subject: help, I've fallen and can't get rid of the precipitate!

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Hello:

After many years of staining grids with uranyl acetate and lead citrate, we
have begun to see a needle like or shard precipitate, (about 1/2 inch long
at 100,000x's; resembles the lead precipitate on page 469 of Electron
Microscopy second edition John Bozzola and Lonnie Russell). We have been
using a 2.5% aqueous uranyl acetate and Reynold's lead citrate (filtered
through a 2 micron filter) for the past 4 years or so with no problems. I
have stained the grids with just UA and can see no precipitate and have
stained grids with just the lead citrate and still not see the precipitate.
I have also checked the water and cannot still see the precipitate.
However, when I stain with UA followed by lead citrate it mysteriously
reappears much to my dissatisfaction. I have also tried the basic lead
citrate and just recently tried Sato's lead stain and had the same problem.
I have made up UA from a newly purchased bottle. I have also lessened the
staining times from 30 minutes in UA to 7 minutes and from 20 minutes in
lead citrate to 5 minutes and the precipitate is less but still there. I
have also checked the grids before staining them and cannot see the
precipitate. Please help, I grow more grey day by day.

Phoebe J. Doss
Manager/Adjunct Instructor
Electron Microscope Lab
Oklahoma State University



From daemon Tue Jun 13 09:42:41 2000



From: Tamara Howard :      howard-at-cshl.org
Date: Tue, 13 Jun 2000 10:31:29 -0400 (EDT)
Subject: Epoxy cleaner?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Back in the olden days, when BioRad sold microscopy supplies, they had an
epoxy cleaner (to remove epoxies from hands, benches, etc., not made from
epoxies). It was blue gunk in a little jar. Does anyone know what happened
to this stuff? Or have an alternate?

I've just been digging through catalogues and Ted Pella sells a liquid
cleaner - any experience with it?

Thanks!

Tamara Howard
CSHL



From daemon Tue Jun 13 10:02:39 2000



From: Ronald LHerault :      lherault-at-bu.edu
Date: Tue, 13 Jun 2000 10:54:11 -0400 (EDT)
Subject: Streptococci Mutans preparation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


a student here needs to fix and dehydrate strep mutans on polystyrene
petri dishes. Can anyone point me to a good (simple?) protocol? Since we
are primarily a materials research lab, we don't have a lot of biological
references.

Thanks

Ron L
lherault-at-bu.edu



From daemon Tue Jun 13 11:22:26 2000



From: Beverly_E_Maleeff-at-sbphrd.com
Date: Tue, 13 Jun 2000 12:15:16 -0400
Subject: Re: Epoxy cleaner?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Tamara:

I don't know the Bio-Rad product, but Ladd sold (still sells?) a product called
Met-a-terge that gets rid of uncured resins. A little bit goes a long way.
I've used it for years.

Hope this helps.

Regards,
Bev Maleeff
SmithKline Beecham Pharmaceuticals




From daemon Tue Jun 13 12:12:21 2000



From: Anthony Garratt-Reed :      tonygr-at-mit.edu
Date: Tue, 13 Jun 2000 13:08:35 -0400
Subject: Transfer of University-owned instrumentation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


John Mansfield's post regarding surplus equipment prompts me to make the
following observations regarding the transfer of University-owned equipment
in the United States. I would imagine that many other countries might have
similar policies.

It often seems to come as a surprise to people to discover that the US
government won't buy the same piece of equipment twice. What I mean by
this is that if a piece of equipment has originally been purchased using
federal funds (regardless of who currently holds title), then another
institution cannot use federal funds (regardless of the source) to buy the
equipment from the first owner.

To use the specific example of John's equipment: suppose it was bought
originally with, say, an NSF grant, and I find that I could make use of it
now. In order to do that I would need to find a non-US-government source
of money, as I could not use even the income from my facility operation
(which is regarded by the accountants as government money, as it originates
predominantly from government research grants).

The logic of this policy, of course, is quite inescapable, however
unpalatable it may be to the present owners of the equipment.

The policy only covers the cost of purchasing the equipment, by the way.
If, to use the same example, John were to give me his surplus equipment, it
would be perfectly acceptable for me to use federal funds to pay for the
packing and shipping (and, if appropriate, reinstallation)

Tony Garratt-Reed.



** Anthony J. Garratt-Reed
** MIT Room # 13-1027
** 77 Massachusetts Avenue
** Cambridge, MA 02139-4307
** USA
**
** Phone: (+) 1-617-253-4622
** Fax: (+) 1-617-258-6479
**




From daemon Tue Jun 13 12:52:15 2000



From: drose-at-wlgore.com
Date: Tue, 13 Jun 2000 13:44:37 -0400
Subject: SEM - sputter coater

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Dear List,

I am using a Denton Desk II sputter coater with a Pt target. I have noticed
recently that a dark spot has formed in the middle of the target. I have not
seen this before. Is this due to impurities in the target, problems with the
vacuum, contamination from outgassing samples, or something else? Everything
appears to be running fine and sample types have not changed. Do I clean it or
just leave it alone? What is it telling me (if the coater could talk)?

Thanks in advance for all your expertise.

David BG Rose
WL Gore and Associates
297 BLue Ball Road
Elkton, MD 21921
410-506-2958




From daemon Tue Jun 13 13:23:51 2000



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Tue, 13 Jun 2000 13:13:50 -0500
Subject: help, I've fallen and can't get rid of the precipitate!

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Phoebe,

We recently had a rash of horrible precipitate problems that we couldn't
seem to trace. Our staining procedures are similar to yours. We made up
fresh stains, changed all our syringe filters, used every precaution we
could think of.

Then we had the water system checked. Our in-line reverse-osmosis,
deionization system had become a mess, although we had assumed (there's that
word!) that the company we leased it from was maintaining it properly.
Turns out that they thought we owned the system, while in fact we only
rented it and paid them to service it.

Anyway, to keep it short, we purchased a Millipore bench-top, low-volume
water-polishing unit and used our old water to feed it (after getting the
thing serviced properly). Our precipitates disappeared and have not yet
reappeared.

For what it's worth.

(No financial interest in Millipore, etc.)

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/




-----Original Message-----
} From: Phoebe J Doss [mailto:pjdoss-at-okstate.edu]
Sent: Tuesday, June 13, 2000 9:33 AM
To: microscopy-at-sparc5.microscopy.com


Hello:

After many years of staining grids with uranyl acetate and lead citrate, we
have begun to see a needle like or shard precipitate, (about 1/2 inch long
at 100,000x's; resembles the lead precipitate on page 469 of Electron
Microscopy second edition John Bozzola and Lonnie Russell). We have been
using a 2.5% aqueous uranyl acetate and Reynold's lead citrate (filtered
through a 2 micron filter) for the past 4 years or so with no problems. I
have stained the grids with just UA and can see no precipitate and have
stained grids with just the lead citrate and still not see the precipitate.
I have also checked the water and cannot still see the precipitate.
However, when I stain with UA followed by lead citrate it mysteriously
reappears much to my dissatisfaction. I have also tried the basic lead
citrate and just recently tried Sato's lead stain and had the same problem.
I have made up UA from a newly purchased bottle. I have also lessened the
staining times from 30 minutes in UA to 7 minutes and from 20 minutes in
lead citrate to 5 minutes and the precipitate is less but still there. I
have also checked the grids before staining them and cannot see the
precipitate. Please help, I grow more grey day by day.

Phoebe J. Doss
Manager/Adjunct Instructor
Electron Microscope Lab
Oklahoma State University



From daemon Tue Jun 13 13:23:51 2000



From: Snow, David B. :      snowdb-at-pweh.com
Date: Tue, 13 Jun 2000 13:17:57 -0500
Subject: Transmission Electron Microscopist (TEM) / Engineer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Transmission Electron Microscopist (TEM) / Engineer

United Technologies Corporation is seeking an engineer to fill the TEM
operator/engineer position at the United Technologies Research Center in
East Hartford, CT. This position will provide support to the United
Technologies Corporation Business Units including Pratt & Whitney, Carrier,
Sikorsky Aircraft, Hamilton Sundstrand, Otis Elevator, and International
Fuel Cells. The TEM operator will be responsible for the dailyoperations of
the TEM laboratory; including preparation of TEM samples using various
techniques such as dimpling, ion milling, jet polishing, microtoming, and
replication. Project duties include conducting failure analyses,
characterization of surface coatings, and analysis of advanced metal and
ceramic materials. The candidate should have experience with both TEM
sample preparation and conventional TEM operation. Good communication and
interpersonal skills are essential. The ability to recognize fracture modes
and origins of fractures is desired. Experience with Scanning Electron
Microscopy (SEM) is a plus.

Qualified candidates will have a BS in Materials Science or equivalent, with
a minimum of 2 years TEM experience. U. S. citizenship or permanent
residency is required.

Please visit our web site at www.utrc.utc.com, and send your resume to
Employment Opportunities, Code MATS-2050-9049, 411 Silver Lane, East
Hartford, CT 06118 or e-mail employment-at-utrc.utc.com. United Technologies
Corporation is an equal opportunity employer.





From daemon Tue Jun 13 16:39:45 2000



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Wed, 14 Jun 2000 08:27:16 GMT+1200
Subject: Re: Epoxy cleaner?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi, Tamara

}
} Back in the olden days, when BioRad sold microscopy supplies, they had an
} epoxy cleaner (to remove epoxies from hands, benches, etc., not made from
} epoxies). It was blue gunk in a little jar. Does anyone know what happened
} to this stuff? Or have an alternate?
}
} I've just been digging through catalogues and Ted Pella sells a liquid
} cleaner - any experience with it?
}

I seem to remember that soaking in N,N-dimethyl formamide dissolves
epoxy, you might care to beg a little from a freiendly chemistry dept
and try it, it's not very expensive. It has a moderately offensive
smeel, though.

rtch


Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand



From daemon Tue Jun 13 16:39:46 2000



From: Ladd Research :      ladres-at-worldnet.att.net
Date: Tue, 13 Jun 2000 16:35:42 -0400
Subject: Re: Epoxy cleaner?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Tamara, Beverly and all other interested parties,

Yes, Ladd stills sells Met-A-Terge (catalog #13045).
Please check our web site http://www.laddresearch.com
for more information or contact me off line.

JD Arnott

Disclaimer: As stated above, Ladd sell Met-A-Terge and thus has a
commercial interest in this thread.

LADD RESEARCH
131 Dorset Lane
Williston, VT 05495 USA

TEL 1-800-451-3406 (US) or 1-802-878-6711 (anywhere)
FAX 1-802-878-8074
e-mail ladres-at-worldnet.att.net

ALL NEW WEB SITE UP NOW AT OUR NEW URL:

http://www.laddresearch.com


Beverly_E_Maleeff-at-sbphrd.com-at-sparc5.microscopy.com wrote:
}
} ------------------------------------------------------------------------} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.}
} Tamara:
}
} I don't know the Bio-Rad product, but Ladd sold (still sells?) a product called
} Met-a-terge that gets rid of uncured resins. A little bit goes a long way.
} I've used it for years.
}
} Hope this helps.
}
} Regards,
} Bev Maleeff
} SmithKline Beecham Pharmaceuticals

--



From daemon Tue Jun 13 16:39:47 2000



From: Jo Dee Fish :      jofish-at-burnham-inst.org
Date: Tue, 13 Jun 2000 13:38:18 -0700
Subject: Re: Epoxy cleaner?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Tamara,
I have used the Ted Pella Epoxy Hand Cleaner and it works well. It will
remove epoxy from hands and also glassware.
Jo Dee Fish

PS I am not affiliated with Ted Pella, just love their products!

Tamara Howard wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Back in the olden days, when BioRad sold microscopy supplies, they had an
} epoxy cleaner (to remove epoxies from hands, benches, etc., not made from
} epoxies). It was blue gunk in a little jar. Does anyone know what happened
} to this stuff? Or have an alternate?
}
} I've just been digging through catalogues and Ted Pella sells a liquid
} cleaner - any experience with it?
}
} Thanks!
}
} Tamara Howard
} CSHL

--
Jo Dee Fish
Electron Microscopy Assistant
Cell Analysis Facility
The Burnham Institute
10901 N. Torrey Pines Rd.
La Jolla, CA 92037
(858)646-3100 ext. 3620




From daemon Tue Jun 13 16:39:51 2000



From: Connie McManus :      conmac-at-cc.usu.edu
Date: Tue, 13 Jun 2000 15:29:58 -0600
Subject: Re: Epoxy cleaner?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


My husband uses epoxy on a sailboat he's building. He cleans everything
up... hands, spills, etc... with plain old vinegar. We go through a LOT of
vinegar. If it's dried, then soak acetone on it until it softens, then use
vinegar (or 5% acetic acid) to mop up the residues.

connie m

At 10:31 AM 06/13/2000 -0400, Tamara Howard wrote:
} Back in the olden days, when BioRad sold microscopy supplies, they had an
} epoxy cleaner (to remove epoxies from hands, benches, etc., not made from
} epoxies). It was blue gunk in a little jar. Does anyone know what happened
} to this stuff? Or have an alternate?
}
} I've just been digging through catalogues and Ted Pella sells a liquid
} cleaner - any experience with it?
}
} Thanks!
}
} Tamara Howard
} CSHL
}
}
}
Connie McManus
Veterinary Diagnostics Lab
Utah State University
Logan, UT
USA



From daemon Tue Jun 13 22:04:13 2000



From: =shAf= :      mshaf-at-darkwing.uoregon.edu
Date: Tue, 13 Jun 2000 16:26:55 -0700
Subject: RE: Transfer of University-owned instrumentation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Anthony Garratt-Reed writes ...

} To use the specific example of John's equipment: suppose
} it was bought originally with, say, an NSF grant, and
} I find that I could make use of it now. In order to do
} that I would need to find a non-US-government source
} of money, as I could not use even the income from my
} facility operation (which is regarded by the accountants
} as government money, as it originates
} predominantly from government research grants).

I would assume it can even get messy if, at least some, of my
facility's income had come from outside sources. I would imagine the
accountants will first assume it is government $$ ... in which case I
would have to show I had taken in an equivelent in outside $$ ... but
over what time frame? ... this fiscal? ... last 10 years?

cheerios, =shAf= :o)

{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {}
Michael Shaffer, R.A. - mshaf-at-darkwing.uoregon.edu
Geological Science's Electron Probe Facility - University of Oregon
http://epmalab.uoregon.edu/




From daemon Tue Jun 13 22:04:28 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Tue, 13 Jun 2000 19:51:28 -0700
Subject: Re: Epoxy cleaner?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I use methylene dichloride (dimethyl chloride) to dissolve most any epoxy.

I have 3-5 gallons of it. I do not know if it is still generally available.

gg

At 07:31 AM 6/13/00, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Tue Jun 13 22:54:38 2000



From: earlw-at-pacbell.net
Date: Tue, 13 Jun 2000 20:43:51 -0700
Subject: Re: MSA Listserver

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Joe,

The listserver is at MSA listserver {Microscopy-at-sparc5.microscopy.com}
Just sent email say, "Please subscribe".
It is fairly active but is only about 1/2 relevant as most data is biology
oriented.

Earl
JCNABITY-at-aol.com wrote:

} Dear Earl,
}
} Could you tell me how to subscribe to the MSA listserver? Greg talked highly
} of it and I'm thinking I will set up another e-mail account to use for it.
} Since it is pretty active, I didn't want all the messages going in with my
} normal e-mail, but a using a separate account will resolve that issue.
}
} Joe



From daemon Wed Jun 14 07:47:22 2000



From: mohamed a jaffer :      mohamed-at-molbiol.uct.ac.za
Date: Wed, 14 Jun 2000 13:45:54 SAST-2
Subject: 70mm roll film

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear List

Our EM Unit has a Zeiss EM109 which uses 70mm roll film - Agfa
Scientia 23D56. Manufacture of this film ceased quite a while back.
We are trying to locate unused stock of this film for our usage. If
you have any surplus stock for sale please contact me.

Thanks
Mohamed

******************************
M. A. Jaffer
Electron Microscope Unit
R. W. James Building
University of Cape Town
Private Bag
Rondebosch, 7701
South Africa

Tel: +27-21-6503354
fax: +27-21-6891528

e-mail: mohamed-at-molbiol.uct.ac.za
***************************************************



From daemon Wed Jun 14 08:50:44 2000



From: Connie McManus :      conmac-at-cc.usu.edu
Date: Wed, 14 Jun 2000 07:47:42 -0600
Subject: Re: Epoxy cleaner?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 06:09 PM 06/13/2000 -0700, Don Hammer wrote:
} Stuff is cheap too and if there is any left after the zillions of home uses,
} great on salads!!!!

yeah, especially the used stuff........ eeeeuewwwwwwww! *G*

connie m
}
} Don Hammer, Retired Guy
} ----- Original Message -----
} From: Connie McManus {conmac-at-cc.usu.edu}
} To: Tamara Howard {howard-at-cshl.org} ; Microscopy Listserver
} {Microscopy-at-sparc5.microscopy.com} ; Histology listserver
} {histonet-at-pathology.swmed.edu}
} Sent: Tuesday, June 13, 2000 2:29 PM
} Subject: Re: Epoxy cleaner?
}
}
} } My husband uses epoxy on a sailboat he's building. He cleans everything
} } up... hands, spills, etc... with plain old vinegar. We go through a LOT
} of
} } vinegar. If it's dried, then soak acetone on it until it softens, then
} use
} } vinegar (or 5% acetic acid) to mop up the residues.
} }
} } connie m
} }
} } At 10:31 AM 06/13/2000 -0400, Tamara Howard wrote:
} } } Back in the olden days, when BioRad sold microscopy supplies, they had an
} } } epoxy cleaner (to remove epoxies from hands, benches, etc., not made from
} } } epoxies). It was blue gunk in a little jar. Does anyone know what
} happened
} } } to this stuff? Or have an alternate?
} } }
} } } I've just been digging through catalogues and Ted Pella sells a liquid
} } } cleaner - any experience with it?
} } }
} } } Thanks!
} } }
} } } Tamara Howard
} } } CSHL
} } }
} } }
} } }
} } Connie McManus
} } Veterinary Diagnostics Lab
} } Utah State University
} } Logan, UT
} } USA
} }
} }
}
}
Connie McManus
Veterinary Diagnostics Lab
Utah State University
Logan, UT
USA



From daemon Wed Jun 14 15:38:17 2000



From: veys-at-bota.ucl.ac.be (Pascal Veys)
Date: Wed, 14 Jun 2000 16:18:08 +0100
Subject: wax and lipid droplets

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi to all,
Does anybody have some information on a possible relationship between lipid
droplets and wax production in plant cells. Are there some evidences that
lipid droplets could actually be storage sites of wax precursors ? I
looked for that in literature but found nothing...
Thus : HELP !
References will be welcome
Thanks in advance for answering
Bye
Pascal

""""""""'
( O)(o )
--------------------0000--------------0000----------
Pascal VEYS
Laboratory of Plant Biology
Catholic University of Louvain
Place Croix du Sud 5 (bte 14)
B 1348 Louvain-la-Neuve
Belgium
Phone : 0032 10473004
Fax : 0032 10473471
Email : Veys-at-bota.ucl.ac.be
---------------------ooooO-----------Ooooo--------
( ) (_)(_) ( )
) ( ) (
(_) (_)




From daemon Wed Jun 14 15:38:17 2000



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Wed, 14 Jun 2000 11:40:12 -0500
Subject: Epoxy removal

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Gary Gaugler wrote:
===============================================================
I use methylene dichloride (dimethyl chloride) to dissolve most any epoxy.
I have 3-5 gallons of it. I do not know if it is still generally available.
================================================================
If we are talking about methylene dichloride, or dichloromethane, CAS # 75-
09-2, this is a pretty bad actor, and is on the list of Prop. 65 chemicals
for the State of California as being cancer causing. Chemicals on the Prop
. 65 list are so highly restricted that in some organizations, they are
allowed in only with the approval of top management.

But I do have a question: I always thought that most epoxies, certainly the
ones used in microscopy, ended up being three dimensionally crosslinked
intractable solids. The only way such a material is going to be "dissolved"
is for chemical bonds to be broken. And yet, I don't see how chemically,
methylene dichloride is going to be breaking chemical bonds. Or the same
comment for some of the other materials mentioned. These materials might
plasticize (e.g. soften) an epoxy and aid in its removal from a surface, but
do any of these really "dissolve" a three dimensionally crosslinked epoxy
system?

I am very interested in this topic because we believe that at least in terms
of getting epoxy out of the "nooks and crannies" of a non-smooth surface, an
oxygen plasma is needed. But perhaps we are wrong about that, that is why
I ask the question.

Disclaimer: SPI Supplies manufactures the Plasma Prep™ II plasma etcher and
has an interest in seeing more applications for plasma etching.

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.spi.cc
########################
============================================








From daemon Wed Jun 14 15:38:19 2000



From: NPGSlithography-at-aol.com
Date: Wed, 14 Jun 2000 13:28:54 EDT
Subject: Please Subscribe

Contents Retrieved from Microscopy Listserver Archives
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Please Subscribe



From daemon Wed Jun 14 15:38:21 2000



From: Joyce Craig :      rcraig8-at-crown.icongrp.com
Date: Wed, 14 Jun 2000 13:50:24 -0500
Subject: SEM-sputter coater

Contents Retrieved from Microscopy Listserver Archives
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David, I had the same problem with a gold target-a dark spot in the
middle. Also, what was being sputtered on my samples was not gold. I
cleaned the target with acetone and it has been working fine since.
Joyce Craig
Chicago State University



From daemon Wed Jun 14 15:38:22 2000



From: Marsha Pilgrim :      mpilgrim-at-MendelBio.COM
Date: Wed, 14 Jun 2000 11:51:50 -0700
Subject: uranyl acetate

Contents Retrieved from Microscopy Listserver Archives
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Hello,

Can anyone recommend a supplier for uranyl acetate?

Also, does anyone out there have experience with Sigma's Lowicryl kit?

If possible, please respond to me directly at: mpilgrim-at-mendelbio.com

Many thanks,
Marsha



From daemon Wed Jun 14 15:38:23 2000



From: Joyce Craig :      rcraig8-at-crown.icongrp.com
Date: Wed, 14 Jun 2000 14:41:55 -0500
Subject: TEM cell cultures

Contents Retrieved from Microscopy Listserver Archives
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We are working with Leishmania major, a parasite that is incorporated
into macrophages. We have had good success with fixation of lymph nodes
infected with Leishmania.
We have not been as happy with the results of fixation of cell cultures
of infected bone marrow macrophages. The membranes of the Leishmania
and of the internal compartments within the macrophages that hold the
Leishmania are well fixed, but the external cell membranes of the
macrophages are somewhat discontiuous. We have not done cell cultures
before. Is this a problem to be expected? We are fixing with 2+2
glutaraldehyde/paraformaldehyde in 0.1 M phosphate buffer, post-fixing
with 2% buffered Osmium, dehydrating with ethanol and propylene oxide,
then embedding in epoxy. We have shortened all times compared to those
we use with tissue samples.
Joyce Craig
Chicago State University



From daemon Wed Jun 14 18:25:47 2000



From: Subramanian Karthikeyan :      karthik-at-micro.uoguelph.ca
Date: Wed, 14 Jun 2000 15:38:48 -0500
Subject: non-florescent plastic coverslip..

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I am looking for a non-fluorescent plastic coverslip that allows
confocal laser microscopy and subsequent sectioning for
Transmission Electron Microscopy. I will greatly appreciate your
suggestions.

Sincerely,
Karthi Subramanian
Department of Microbiology
University of Guelph
Guelph Ontario N1G 2W1
Phone: (519)824-4120 ext.8904
Fax:(519)837-1802




From daemon Wed Jun 14 18:25:48 2000



From: Dusevich, Vladimir :      DusevichV-at-umkc.edu
Date: Wed, 14 Jun 2000 15:46:01 -0500
Subject: RE: Stereometry by computer

Contents Retrieved from Microscopy Listserver Archives
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I perform stereo measurements only occasionally,
so I prefer to save money on specialized equipment or
software. All I use is just freeware program ImageTool
(good for on-screen stereo pair measurements) and Excel
(not bad for calculations).

Of course, for a big project it's better to buy a software.

Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
3127 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy

} -----Original Message-----
} From: John J. Bozzola [mailto:bozzola-at-siu.edu]
} Sent: Thursday, June 08, 2000 11:13 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Stereometry by computer
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
}
} --------------------------------------------------------------
} ---------.
}
}
} A user of our facility is interested in making height measurements
} from specimens viewed in the SEM. I am aware of the conventional way
} of doing this: stereo pairs and optical viewer (with stereometer
} parallax corrections). Is there a more modern (computerized) way of
} doing this, say with anaglyphs?
}
} Thank you.
}
} John B.
}
}
} ####################################################################
} John J. Bozzola, Ph.D., Director
} Micro-Imaging and Analysis Center
} 750 Communications Drive - MC 4402
} Southern Illinois University
} Carbondale, IL 62901 U.S.A.
} Phone: 618-453-3730
} Fax: 618-453-2665
} Email: bozzola-at-siu.edu
} Web: http://www.siu.edu/departments/shops/cem.html
} ####################################################################
}



From daemon Wed Jun 14 18:25:48 2000



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Wed, 14 Jun 2000 10:48:50 -1000 (HST)
Subject: TEM labeling Si-OH groups?

Contents Retrieved from Microscopy Listserver Archives
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Oh wise and helpful microscopists-

I need to label Si-OH groups with something that will show up in TEM. If
it were proteins or sugars, I'd immuno- or lectin label. Any ideas how I
can get gold or ferritin or whatever onto these groups?

Mahalo!
Tina

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************



From daemon Wed Jun 14 18:25:48 2000



From: Ladd Research :      ladres-at-worldnet.att.net
Date: Wed, 14 Jun 2000 17:03:40 -0400
Subject: Re: uranyl acetate

Contents Retrieved from Microscopy Listserver Archives
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Marsha Pilgrim wrote:

} Hello,
}
} Can anyone recommend a supplier for uranyl acetate?
} Also, does anyone out there have experience with Sigma's Lowicryl kit?
} If possible, please respond to me directly at: mpilgrim-at-mendelbio.com
}
} Many thanks,
} Marsha


Dear Marsha,

We at Ladd Research, and most of the other supply companies, can sell
you this. In our case it is catalog # 23620 and more information can be
found on our web site, http://www.laddresearch.com

John Arnott
--

LADD RESEARCH
131 Dorset Lane
Williston, VT 05495 USA

TEL 1-800-451-3406 (US) or 1-802-878-6711 (anywhere)
FAX 1-802-878-8074
e-mail ladres-at-worldnet.att.net

ALL NEW WEB SITE UP NOW AT OUR NEW URL:

http://www.laddresearch.com



From daemon Wed Jun 14 18:25:49 2000



From: beth-at-dogwood.botany.uga.edu (Beth Richardson)
Date: Wed, 14 Jun 2000 17:49:03 -0500
Subject: autofluorescence of plant material

Contents Retrieved from Microscopy Listserver Archives
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Help - A faculty member's plant tissue is autofluorescing. She is using
aniline blue to look at pollen tubes (through the styles). She would like
to reduce the background fluorescence. I gave her a copy of a borohyride
reference from a '97 listserv posting. Can anyone recommend a fixation that
will decrease or eliminate the autofluorescence? Any thoughts on this
matter would be greatly appreciated.
thanks,
Beth

**************************************
Beth Richardson
EM Lab Coordinator
Botany Department
University of Georgia
Athens, GA 30602

Phone - (706) 542-1790
FAX - (706) 542-1805
Email - beth-at-dogwood.botany.uga.edu
**************************************




From daemon Wed Jun 14 18:25:50 2000



From: Heather A Owen :      owenha-at-csd.uwm.edu
Date: Wed, 14 Jun 2000 16:47:36 -0500 (CDT)
Subject: Re: LR White flat embedding

Contents Retrieved from Microscopy Listserver Archives
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It took me a few days to find this protocol from my collegue Lucinda
Swatzell. It sounded intriguing. Although I've not tried it myself yet,
she's used it with success.

I've copied the following out of her e-mail. Things that need to be kept
in mind are that she polymerizes in a vacuum oven, she's refering to plant
seedlings, and that I don't have any financial interest that I know of
(i.e. I haven't checked my mutual fund prospectus) in Rubbermaid, Inc.

Here it is:

"There is a way to flat embed with LR White: This works great for me when
I am keeping them on their agar blocks and maintaining orientation, but it
should also work for regular seedlings to keep them flat instead of
crooked in the bottom of the capsules. Use rubbermaid ice cube trays.
Place the specimens in the flat bottoms of the tray. cover with about 1/4
in of resin. In each well, place another well that has been cut from it's
tray. The single loose wells will nestle down into the ice cub tray on
top of the resin. Because rubbermaid is dishwasher safe it will take the
heat, but get soft enough to snuggle in tightly and keep out oxygen. When
you pump the vaccuum the extra resin also snakes up into the cracks, so
that you get a seal. Now the thing that is scary: will loose seedlings
just suck up into the cracks? I haven't tried them."

Heather A. Owen, Director
Electron Microscope Laboratory
Department of Biological Sciences
University of Wisconsin - Milwaukee
(414)229-6816





From daemon Wed Jun 14 18:25:50 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Wed, 14 Jun 2000 15:07:34 -0700
Subject: Re: Epoxy removal

Contents Retrieved from Microscopy Listserver Archives
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I've used it often to unpackage epoxy microchips. These
epoxies are silicone/epoxy and not true epoxy. They are
typically called plastic packaged ICs. Whatever. I've
used MEC and DiMEC to do this. Some packages
had to be oxygen ion blasted open. Over time, the plasma
method has been much safer for the operator and the chip.
And it can make the removal process much faster. Plasma
is the current method of choice.

Whether the "epoxies" talked about here are the same as
those for IC packages is likely not to be.

gary g.



At 09:40 AM 6/14/00, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Wed Jun 14 18:25:51 2000



From: Walck, Scott D. :      walck-at-ppg.com
Date: Wed, 14 Jun 2000 18:26:55 -0400
Subject: RE: Jet polishing of tungsten

Contents Retrieved from Microscopy Listserver Archives
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I used to prepare W FIM emitter tips with a 5% NaOH solution and I think the
voltage was around 5 to 10 volts ac. When I used to electropolish, the FIM
solutions and conditions were frequently similar to the jet polishing
solutions.


-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)



} -----Original Message-----
} From: Gillian Bond [mailto:gbond-at-nmt.edu]
} Sent: Monday, June 12, 2000 7:43 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Jet polishing of tungsten
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} ---------.
}
}
}
} I have a student who has been trying to jet polish tungsten
} for TEM. She
} has tried various concentrations of sodium hydroxide in
} water, as well as
} 40g trisodium phosphate/250ml water, and 55.8g magnesium
} perchlorate/250ml
} methanol, at a range of voltages. We have a Fischione jet-polishing
} unit. So far, none of the samples has been close to good. Can anyone
} help us out here, with past experience or general suggestions?
}
} Many thanks in advance,
}
} Gill
}
} Dr Gillian M. Bond
} Department of Materials & Metallurgical Engineering
} New Mexico Tech
} Socorro, NM 87801
}
}



From daemon Thu Jun 15 07:33:49 2000



From: Thomas Neumann :      tneumann-at-u.washington.edu
Date: Wed, 14 Jun 2000 16:43:29 -0700
Subject: Artifact or Structured Water?

Contents Retrieved from Microscopy Listserver Archives
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My name is Tim Strovas and I am a graduate student at the U of
Washington bioengineering department. I am investigating the halo
effect from single myofibrils (from bumblebee muscle tissue) as seen
under a phase contrast microscope. Has anyone encountered a previous
investigation into this effect and its possible relationship with
structured water?
Note: The Halo does not appear as ripples that are normally associated
with optical microscope artifacts. The halo is single broad band that
borders the tissue sample.




From daemon Thu Jun 15 07:33:49 2000



From: Thomas Neumann :      tneumann-at-u.washington.edu
Date: Wed, 14 Jun 2000 16:54:35 -0700
Subject: Artifact or Structured Water?

Contents Retrieved from Microscopy Listserver Archives
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Sorry, forgot return address: T-Strovas-at-Cornell-Iowa.edu

My name is Tim Strovas and I am a graduate student at the U of
Washington bioengineering department. I am investigating the halo
effect from single myofibrils (from bumblebee muscle tissue) as seen
under a phase contrast microscope. Has anyone encountered a previous
investigation into this effect and its possible relationship with
structured water?
Note: The Halo does not appear as ripples that are normally associated
with optical microscope artifacts. The halo is single broad band that
borders the tissue sample.





From daemon Thu Jun 15 07:33:50 2000



From: Thomas Neumann :      tneumann-at-u.washington.edu
Date: Wed, 14 Jun 2000 16:54:23 -0700
Subject: Artifact or Structured Water?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Sorry, forgot return address: T-Strovas-at-Cornell-Iowa.edu

My name is Tim Strovas and I am a graduate student at the U of
Washington bioengineering department. I am investigating the halo
effect from single myofibrils (from bumblebee muscle tissue) as seen
under a phase contrast microscope. Has anyone encountered a previous
investigation into this effect and its possible relationship with
structured water?
Note: The Halo does not appear as ripples that are normally associated
with optical microscope artifacts. The halo is single broad band that
borders the tissue sample.





From daemon Thu Jun 15 07:33:50 2000



From: =?iso-2022-jp?B?GyRCI1IjaSN6I2EjbBsoSg==?=
Date: Thu, 15 Jun 2000 09:08:35 +0900
Subject: unsubscribe

Contents Retrieved from Microscopy Listserver Archives
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From daemon Thu Jun 15 07:33:51 2000



From: John Nailon :      mmjnailo-at-dingo.cc.uq.edu.au
Date: Thu, 15 Jun 2000 11:35:56 +1000
Subject: Re: Epoxy cleaner?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


G'day folks,
Be very wary of any Good Epoxy Solvent, do not use it to clean epoxy off
your skin. Anything that is a good solvent for epoxy will probably be a
good solvent for the oils and lipids in/on your skin. These oils and
lipids are your protection against epoxy resins entering your body.
Remember, all epoxy resins at carcinogenic, soap and water are probably
the safest agents to remove epoxies from your skin.
Regards
JVN

Connie McManus wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} At 06:09 PM 06/13/2000 -0700, Don Hammer wrote:
} } Stuff is cheap too and if there is any left after the zillions of home uses,
} } great on salads!!!!
}
} yeah, especially the used stuff........ eeeeuewwwwwwww! *G*
}
} connie m
} }
} } Don Hammer, Retired Guy
} } ----- Original Message -----
} } From: Connie McManus {conmac-at-cc.usu.edu}
} } To: Tamara Howard {howard-at-cshl.org} ; Microscopy Listserver
} } {Microscopy-at-sparc5.microscopy.com} ; Histology listserver
} } {histonet-at-pathology.swmed.edu}
} } Sent: Tuesday, June 13, 2000 2:29 PM
} } Subject: Re: Epoxy cleaner?
} }
} }
} } } My husband uses epoxy on a sailboat he's building. He cleans everything
} } } up... hands, spills, etc... with plain old vinegar. We go through a LOT
} } of
} } } vinegar. If it's dried, then soak acetone on it until it softens, then
} } use
} } } vinegar (or 5% acetic acid) to mop up the residues.
} } }
} } } connie m
} } }
} } } At 10:31 AM 06/13/2000 -0400, Tamara Howard wrote:
} } } } Back in the olden days, when BioRad sold microscopy supplies, they had an
} } } } epoxy cleaner (to remove epoxies from hands, benches, etc., not made from
} } } } epoxies). It was blue gunk in a little jar. Does anyone know what
} } happened
} } } } to this stuff? Or have an alternate?
} } } }
} } } } I've just been digging through catalogues and Ted Pella sells a liquid
} } } } cleaner - any experience with it?
} } } }
} } } } Thanks!
} } } }
} } } } Tamara Howard
} } } } CSHL
} } } }
} } } }
} } } }
} } } Connie McManus
} } } Veterinary Diagnostics Lab
} } } Utah State University
} } } Logan, UT
} } } USA
} } }
} } }
} }
} }
} Connie McManus
} Veterinary Diagnostics Lab
} Utah State University
} Logan, UT
} USA

--
****************************************************
John V Nailon
Operations Manager
Centre for Microscopy and Microanalysis
The University of Queensland
St. Lucia Queensland 4072
Phone: +61-7-3365-4214
Fax: +61-7-3365-4422
WWW: http://www.uq.edu.au/nanoworld/allstaff.html#Nailon
****************************************************



From daemon Thu Jun 15 07:34:12 2000



From: Rick Powell at Nanoprobes :      rpowell-at-nanoprobes.com
Date: Thu, 15 Jun 2000 06:38:06 -0500
Subject: Re: TEM labeling Si-OH groups?

Contents Retrieved from Microscopy Listserver Archives
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I suggest you copy this message to the plant surfaces mail list

To join, send the command
join plant-surfaces firstname lastname
to: mailbase-at-mailbase.ac.uk
"Firstname" can be one or more names or initials. The last
word in this command will be interpreted as the last name.
The email address will be extracted automatically from the
message.

Chris Jeffree

Date sent: Wed, 14 Jun 2000 16:18:08 +0100
To: microscopy-at-sparc5.microscopy.com
} From: veys-at-bota.ucl.ac.be (Pascal Veys)


Hello Tina:

I would approach your problem by either modifying the Si-OH groups with a
hapten and detecting with antibody- or streptavidin-gold, or converting
them to amines or thiols then labeling with a gold labeling reagent
(disclaimer - we make gold labeling reagents). You could introduce amino-
groups at the Si-OH groups using a silylating reagent such as
3-{Tris[2-(2-methoxyethoxy)ethoxy]silyl}propylamine or
3-[Tris(trimethylsiloxy)silyl]propylamine (both from Fluka), then either
biotinylate with NHS-biotin and detect with streptavidin-gold, or label the
amines with Mono-Sulfo-NHS-Nanogold.

I have not actually tried this, and since I don't know what types of
samples you are looking at, it's difficult to say what else in them might
affect the reaction. If there are already other primary amines in your
sample, they need to be blocked first.

If you would like other ideas, a text on solid-phase oligo- or peptide
synthesis might be another good starting point - the chemistry used to
functionalize the beads used in these systems may also be transferable to
your situation.

Hope this is helpful,

Rick Powell


}
} Oh wise and helpful microscopists-
}
} I need to label Si-OH groups with something that will show up in TEM. If
} it were proteins or sugars, I'd immuno- or lectin label. Any ideas how I
} can get gold or ferritin or whatever onto these groups?
}
} Mahalo!
} Tina
}
} ****************************************************************************
} * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
} * Biological Electron Microscope Facility * (808) 956-6251 *
} * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
} ****************************************************************************

**********************************************************************
* NANOPROBES, Incorporated | Tel: (919) 510-0590 *
* 95 Horse Block Road | Fax: (919) 510-0590 *
* Yaphank, NY 11980-9710, | rpowell-at-nanoprobes.com *
* USA | www.nanoprobes.com *
**********************************************************************




From daemon Thu Jun 15 20:52:27 2000



From: jim :      jim-at-proscitech.com.au
Date: Thu, 15 Jun 2000 22:57:27 +1000
Subject: RE: Epoxy cleaner?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


John Nailon is making a very good argument for using gloves or working very
cleanly.
All epoxies I understand are "somewhat" carcinogenic. The much quoted John
Luft, years ago advised me that photographic fixer (sodium thiosulphate)
solution, chemically changed epoxies so they would not be carcinogenic. If he
was right, then first washing any body parts contaminated by epoxy resin in
photographic fixer should avert the worse. Those fixers do not dissolve or
clean epoxies.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Thursday, June 15, 2000 11:36 AM, John Nailon
[SMTP:mmjnailo-at-dingo.cc.uq.edu.au] wrote:
}
} G'day folks,
} Be very wary of any Good Epoxy Solvent, do not use it to clean epoxy off
} your skin. Anything that is a good solvent for epoxy will probably be a
} good solvent for the oils and lipids in/on your skin. These oils and
} lipids are your protection against epoxy resins entering your body.
} Remember, all epoxy resins at carcinogenic, soap and water are probably
} the safest agents to remove epoxies from your skin.
} Regards
} JVN
}
} Connie McManus wrote:
} }
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} } At 06:09 PM 06/13/2000 -0700, Don Hammer wrote:
} } } Stuff is cheap too and if there is any left after the zillions of home
} } } uses,
} } } great on salads!!!!
} }
} } yeah, especially the used stuff........ eeeeuewwwwwwww! *G*
} }
} } connie m
} } }
} } } Don Hammer, Retired Guy
} } } ----- Original Message -----
} } } From: Connie McManus {conmac-at-cc.usu.edu}
} } } To: Tamara Howard {howard-at-cshl.org} ; Microscopy Listserver
} } } {Microscopy-at-sparc5.microscopy.com} ; Histology listserver
} } } {histonet-at-pathology.swmed.edu}
} } } Sent: Tuesday, June 13, 2000 2:29 PM
} } } Subject: Re: Epoxy cleaner?
} } }
} } }
} } } } My husband uses epoxy on a sailboat he's building. He cleans everything
} } } } up... hands, spills, etc... with plain old vinegar. We go through a LOT
} } } of
} } } } vinegar. If it's dried, then soak acetone on it until it softens, then
} } } use
} } } } vinegar (or 5% acetic acid) to mop up the residues.
} } } }
} } } } connie m
} } } }
} } } } At 10:31 AM 06/13/2000 -0400, Tamara Howard wrote:
} } } } } Back in the olden days, when BioRad sold microscopy supplies, they had
} } } } } an
} } } } } epoxy cleaner (to remove epoxies from hands, benches, etc., not made
} } } } } from
} } } } } epoxies). It was blue gunk in a little jar. Does anyone know what
} } } happened
} } } } } to this stuff? Or have an alternate?
} } } } }
} } } } } I've just been digging through catalogues and Ted Pella sells a liquid
} } } } } cleaner - any experience with it?
} } } } }
} } } } } Thanks!
} } } } }
} } } } } Tamara Howard
} } } } } CSHL
} } } } }
} } } } }
} } } } }
} } } } Connie McManus
} } } } Veterinary Diagnostics Lab
} } } } Utah State University
} } } } Logan, UT
} } } } USA
} } } }
} } } }
} } }
} } }
} } Connie McManus
} } Veterinary Diagnostics Lab
} } Utah State University
} } Logan, UT
} } USA
}
} --
} ****************************************************
} John V Nailon
} Operations Manager
} Centre for Microscopy and Microanalysis
} The University of Queensland
} St. Lucia Queensland 4072
} Phone: +61-7-3365-4214
} Fax: +61-7-3365-4422
} WWW: http://www.uq.edu.au/nanoworld/allstaff.html#Nailon
} ****************************************************



From daemon Thu Jun 15 20:52:28 2000



From: Smartech :      smartech-at-javanet.com
Date: Thu, 15 Jun 2000 10:02:36 -0400
Subject: SEM, How to interpret a fractured adhesive film

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I needed to have a cross sectional view into an adhesive layer so that I
could count the layers, if possible. Since the adhesive acts like a highly
viscous liquid, polishing it is out of the question. Instead, I submerged
the film into liquid Nitrogen and cut it quickly w/ a scissors. The sound
of the cut was more like a breaking sound. When I examined the cross
sectional surface I observed a brain like surface. There were columns with
a highly consistent diameter and orientation. It looked crystalline. There
was zero evidence of material smearing that one would expect if one cut a
material. Is the proper interpretation that these columns existed before
the fracture and that the fracture occurred along the boundaries? Or is
the cross sectional surface generated by a rippled distortion of an highly
viscous liquid? The regularity of the surface seems to make the latter
interpretation unlikely.

I would be interested in any opinions on the interpretation of the image or
alternative means of cross sectioning the adhesive layer.

I could e-mail an image to anyone who is interested.

Thanks

Ric



From daemon Thu Jun 15 20:52:30 2000



From: diane.a.ciaburri-at-gdds.com
Date: 6/14/00 3:07 PM
Subject: Re: Epoxy removal

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Gary and others,

We haven't had a need to unpackage 'plastic' encapsulated IC's for a while but
may have a need to do so soon (preferrably not impairing functionality?!). I am
told that the last person to do this here (now retired) dripped fuming sulfuric
acid on the plastic and used frequent water rinses. We have a plasma etcher but
I was afraid it would take forever to get through the plastic.

Any hints and suggestions would be greatly appreciated.

Diane Ciaburri
Senior Materials Engineer
General Dynamics
100 Plastics Ave.
Pittsfield MA 01210

____________________Forward Header_____________________

I've used it often to unpackage epoxy microchips. These
epoxies are silicone/epoxy and not true epoxy. They are
typically called plastic packaged ICs. Whatever. I've
used MEC and DiMEC to do this. Some packages
had to be oxygen ion blasted open. Over time, the plasma
method has been much safer for the operator and the chip.
And it can make the removal process much faster. Plasma
is the current method of choice.

Whether the "epoxies" talked about here are the same as
those for IC packages is likely not to be.

gary g.




From daemon Thu Jun 15 20:52:30 2000



From: Hao Li :      haoli-at-glue.umd.edu
Date: Thu, 15 Jun 2000 10:40:15 -0700
Subject: information about MgAl2O4

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Hi, All:

This one is not really related to microscopy. But since I am a TEM guy, I
hope I can get some help here.

Does anybody have some information about MgAl2O4 as a substrate for
perovskite films? I know it is not often used, so I am worndering if it has
a major disadvantage so that nobody is using it. Any references would be
welcome.


Thanks a lot

Hao Li



From daemon Thu Jun 15 20:52:31 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Thu, 15 Jun 2000 07:55:38 -0700
Subject: Polaron E5200 control board

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have a control PC board for the E5200 sputter coater.
This is the model with an Intel single chip MPU on one
end and a 4-conductor socket on the other end. The
board uses a VME connector for main interface.

Coater is trashed. Board is OK. If anybody can use
the board, first request gets it.

gary g.



From daemon Thu Jun 15 20:52:33 2000



From: Andy Horsewell :      horsewell-at-ipt.dtu.dk
Date: Thu, 15 Jun 2000 19:05:03 +0200
Subject: please unsubscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Please unsubscribe



From daemon Thu Jun 15 20:52:34 2000



From: Mary C. Pfauth :      mpfauth-at-teleport.com
Date: Thu, 15 Jun 2000 09:53:28 -0700 (PDT)
Subject: Re: autofluorescence of plant material

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Beth, more details are needed on how the tissue was processed before
viewing. I have used aniline blue to view pollen tubes in style of
Salicornia virginica which had ben fixed in Nawashin's fixative. Have also
viewed tubes of Melilotus which had simply been preserved in 70% EtOH. If
one uses glut as a fixative, it fluoresces so you won't be able to
distinguish the PT from everything else. Mary Pfauth

John P.B. & Mary
mpfauth-at-teleport.com



From daemon Thu Jun 15 20:52:35 2000



From: White, Woody N :      Woody.N.White-at-mcdermott.com
Date: 6/14/00 3:07 PM
Subject: Re: Epoxy removal

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Can't comment on sulfuric, but I have used red fuming nitric at near boiling
temperature. Apply acid, let react. Flush witn more acid, let react, etc.

Woody White

The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Gary and others,

We haven't had a need to unpackage 'plastic' encapsulated IC's for a while
but
may have a need to do so soon (preferrably not impairing functionality?!).
I am
told that the last person to do this here (now retired) dripped fuming
sulfuric
acid on the plastic and used frequent water rinses. We have a plasma etcher
but
I was afraid it would take forever to get through the plastic.

Any hints and suggestions would be greatly appreciated.

Diane Ciaburri
Senior Materials Engineer
General Dynamics
100 Plastics Ave.
Pittsfield MA 01210

____________________Forward Header_____________________

I've used it often to unpackage epoxy microchips. These
epoxies are silicone/epoxy and not true epoxy. They are
typically called plastic packaged ICs. Whatever. I've
used MEC and DiMEC to do this. Some packages
had to be oxygen ion blasted open. Over time, the plasma
method has been much safer for the operator and the chip.
And it can make the removal process much faster. Plasma
is the current method of choice.

Whether the "epoxies" talked about here are the same as
those for IC packages is likely not to be.

gary g.



From daemon Thu Jun 15 20:52:41 2000



From: Yan Xin :      xin-at-magnet.fsu.edu
Date: Thu, 15 Jun 2000 16:29:08 -0400
Subject: modulus for LaAlO3

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X-Mailer: QUALCOMM Windows Eudora Pro Version 4.2.0.58


Hello,

Does anyone know the shear modulus for LaAlO3?

Thanks

Yan Xin
=======================================
Yan Xin
Magnet Science & Technology
National High Magnetic Field Laboratory
Florida State University
1800 E. Paul Dirac Drive
Tallahassee, FL 32310
Tel: (850) 644 1529
Fax: (850) 644 0867
========================================





From daemon Thu Jun 15 20:52:41 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Thu, 15 Jun 2000 13:21:14 -0700
Subject: Re: Epoxy removal from ICs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The ion beam approach works well. I have not used it
recently on finer pitch ICs. With as-built feature sizes
of 2-4u, it is fine. It will stop at the passivation and leave
the Al bond wires intact. The resulting package looks like
it has a V-shaped pit in it (which it does). The extent of the
pit depends on the size of the die and if you want to blast
down to the lead frame or substrate.

I have not done this on finer pitch devices. I would be a
bit skeptical about these mostly because of the smaller
bond pads. The etching would still stop at the passivation.

There are numerous places in Silicon Valley that do this
on an outsource basis. Typical costs are about $75 per IC.
I can get some contacts for you if you'd like.

gary g.



At 06:55 AM 6/15/00, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Thu Jun 15 20:52:42 2000



From: Barbara Foster :      mme-at-map.com
Date: Thu, 15 Jun 2000 16:46:47 -0400
Subject: Impact of the Internet

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


MME is currently conducting research through Microscopy & Analysis
regarding the impact of the internet on microscopy and imaging facilities.
If you have not yet faxed back your responses, we'd appreciate your
participation. The questionnaire is in the center of the May issue of M&A.

Results of this survey will be reported in a Fall issue of Microscopy &
Analysis; specific information on the impact of the internet will be
presented along with data collected from other recent MME surveys and
reports from other meetings in the "Microbrew" column in the July issue of
Advanced Imaging.

Many thanks.

Best regards,
Barbara Foster
Microscopy/Marketing & Education
125 Paridon Street, Suite 102
Springfield, MA 01118-2130
PH: 413-746-6931 FX: 413-746-9311 email:mme-at-map.com

Contributing editor: Advanced Imaging "MicroBrew"



From daemon Thu Jun 15 20:52:44 2000



From: ERIC :      biology-at-ucla.edu
Date: Thu, 15 Jun 2000 14:29:48 -0700
Subject: Osmium Fixation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


To the wealth of knowledge on the Microscopy list server,

I have a question about Osmium fixation..Basically I was curious to know
if there are any references to Osmium fixation at room temperature?
Everything I have seen so far only talks about fixation for 2 hours in the
refrigerator at 4 degrees...

Are there any drawback or problems that can occur if tissues specifically
Kidney and muscle would or could have? i.e. precipitation etc... etc....

Thanks in advance,

Eric
UCLA Medical Center





From daemon Thu Jun 15 20:52:46 2000



From: DAVID I SAXON :      DISAXON-at-prodigy.net
Date: Fri, 16 Jun 2000 03:38:26 -0700
Subject: Re: epoxy removal from ICs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


}
} We haven't had a need to unpackage 'plastic' encapsulated IC's for a while but
} may have a need to do so soon (preferrably not impairing functionality?!). I am
} told that the last person to do this here (now retired) dripped fuming sulfuric
} acid on the plastic and used frequent water rinses. We have a plasma etcher but
} I was afraid it would take forever to get through the plastic.
}
} Any hints and suggestions would be greatly appreciated.
}
} Diane Ciaburri
} Senior Materials Engineer
} General Dynamics
} 100 Plastics Ave.
} Pittsfield MA 01210

Diane,
Yes, hot fuming sulfuric and/or hot fuming nitric are used as standard
procedures for removing plastic from IC's. The process is not quite that
simple. For example, water rinses will almost certainly etch the bond pads
on the IC and thus removing connection to the outside world. Additionally,
the plastic contains fire retardants which some regions don't like being
washed down the drain. There is more detailed help through EDFAS.org (one
of ASM's branches). B&G International sells a very safe, effective etcher
which performs decapsulation automatically in minutes.

I have no association with B&G International.

David Saxon
Analytical Microscope Services
11826 Reservoir Rd. E.
Puyallup, WA 98374
253-848-7701 voice & fax
email: info-at-analyticalmicroscope.com
website: www.analyticalmicroscope.com



From daemon Thu Jun 15 20:52:49 2000



From: Robert Blystone :      rblyston-at-trinity.edu
Date: Thu, 15 Jun 2000 18:31:30 -0500
Subject: OnCor

Contents Retrieved from Microscopy Listserver Archives
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To the LIST:

I am looking for information about an analysis software package by the
name of ONCOR. Does any have an adress for the company.... which may not
exist anymore?? Thanks
Blystone in Texas

Robert V. Blystone, PH.D.
Professor of Biology
Trinity University
San Antonio, Texas 78212
rblyston-at-trinity.edu
210-999-7243 FAX 210-999-7229



From daemon Thu Jun 15 21:23:00 2000



From: tbonner :      tbonner-at-brockport.edu
Date: Thu, 15 Jun 2000 21:14:18 -0500
Subject: Micrographs from digital

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Being unable to afford a digital camera for my TEM. I'm wondering if the next
best option is to get a high end scanner to scan in negatives and then print
them on a decent printer. Any advice regarding this idea and brands of
scanners and printers that are useful? Also, what image analysis systems are
user friendly?

Dr. Thomas P. Bonner
Department of Biological Sciences
SUNY at Brockport
Brockport, NY 14420




From daemon Thu Jun 15 21:23:01 2000



From: Carrie Golash :      cdg126-at-psu.edu
Date: Thu, 15 Jun 2000 21:13:34 -0500
Subject: Embedding oocytes in araldite

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hello all -

I'm looking for advice on embedding bovine oocytes (~100 micron
diameter).
I'd like to embed them in araldite for probing with labelled lectins as
this seems to be fairly well established in the literature. The hangup is
this: we are using fairly large plastic cassettes and are having problems
losing the oocytes within the volume of araldite. Does anyone know of a way
around this? Is there something we can pre-embed the oocytes in to make a
smaller chip that we can then embed in the larger block (that is, of
course, compatible with polymerizing / clearing the araldite? Or does
anyone know of an altogether different method for oocyte embedding that is
more effective? Thanks in advance!

--Carrie Golash

Carrie Golash
John O. Almquist Research Center
Penn State University
University Park, PA 16802
W: (814) 865-5896
H: (814) 692-7926
http://www.das.psu.edu/dbrc/dbrc.htm




From daemon Fri Jun 16 08:24:44 2000



From: D.kierst :      dlkierst-at-config.com
Date: Fri, 16 Jun 2000 00:32:14 -0400
Subject: RE: Wanted Atomic Force Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Our materials science microscopy lab is in need of a used atomic force
microscope. No specific model or make. All reasonable offers will be
considered. Please respond directly to Don Kierstead at or call
330-794-6600. Any help in this effort would be greatly appreciated.



From daemon Fri Jun 16 08:24:45 2000



From: John Twilley :      jtwilley-at-sprynet.com
Date: Fri, 16 Jun 2000 00:53:53 -0400
Subject: Re: epoxy removal from ICs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Perhaps it's time to comment on this thread.

Dichloromethane and dimethylformamide are relatively effective disrupters of most
epoxies but their action is accompanied by great swelling because the polymer becomes
engorged with the liquid before any significant solvation takes place. This will
destroy wire bonds on an IC.

Fuming (essentially anhydrous) sulfuric acid acts by the completely different process
of sulfonating reactive groups that remain on the polymer. The depolymerized and
sulfonated byproducts are quite soluble not only in the acid but usually in water as
well. The worst thing that you could do in this relatively straightforward process
is to wash with water at intervals because this would initiate almost instantaneous
corrosion. It would be advisable for a chemist, as someone trained in the handling
of reactive materials, to carry this out or at least to establish procedures and
train others with less experience. The action of sulfuric acid in this regard is
quite different than that of nitric. Nearly anhydrous nitric acid (completely
anhydrous is extremely difficult to prepare) is a very powerful oxidizer and could
lead to unstable, dangerous byproducts whereas the sulfonates resulting from the
sulfuric acid reaction are relatively stable. Water must, of course, be prevented
from splashing into any concentrated acid, especially sulfuric.

A very strong acid such as sulfuric behaves completely differently in the absence of
water. Since most acids are highly hygroscopic and are sold as water solutions, most
people do not observe this other side of their behavior. Without water to create an
ionized electrolyte, corrosion of metals will not take place. I have de-encapsulated
ICs for failure analysis in 200 degree sulfuric acid and been able to operate the IC
without replacing the .001" aluminum wirebonds that it came with. I recall one
instance where our company built prototype hybrid microelectronic circuits out of
such de-encapsulated ICs when their supplier was late getting a new design on the
market and the only ones available were already encapsulated.

The key is to realize that water must be excluded until the sulfonating acid has been
completely rinsed away by a non-aqueous liquid. As Mr. Saxon said, there are simple
and safe devices available for doing this operation. However, with proper care and
protective gear it can be done in a beaker on a hot plate in a fume hood. A few ml.s
of sulfuric acid are heated to drive off water until heavy vapors are observed over
the liquid (which may darken during heating due to trace impurities). The IC is
carefully lowered into the hot acid and a vigorous reaction ensues with the epoxy
almost instantly washing into the solution. After a few seconds the IC is then
quickly lifted out and held over a receiving vessel and flooded with a stream of
ethanol. Only after this is a final rinse in deionized water carried out, followed
by fresh electronic grade ethanol and forced drying in warm air.

The ready made devices which carry out the operation are typically a small bowl with
a hinged lid from which air is withdrawn by a gentle vacuum. An inert metal feeder
tube leads from a heated reservoir for the sulfuric acid and passes through the wall
of the bowl to a position where the encapsulated device is secured. When the lid is
closed and the slight vacuum applied, the hot acid is pulled into the bowl over the
device. It is somewhat self-limiting in that, if the lid is opened, there is no
driving force to bring more acid into the container. Naturally, the vacuum source
needs to be protected by a trap and all waste products properly handled no matter how
the procedure is carried out.

John Twilley
Conservation Scientist
(formerly, Manager of the Reliability Analysis Center,
Teledyne Microelectronics)

DAVID I SAXON wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} }
} } We haven't had a need to unpackage 'plastic' encapsulated IC's for a while but
} } may have a need to do so soon (preferrably not impairing functionality?!). I am
} } told that the last person to do this here (now retired) dripped fuming sulfuric
} } acid on the plastic and used frequent water rinses. We have a plasma etcher but
} } I was afraid it would take forever to get through the plastic.
} }
} } Any hints and suggestions would be greatly appreciated.
} }
} } Diane Ciaburri
} } Senior Materials Engineer
} } General Dynamics
} } 100 Plastics Ave.
} } Pittsfield MA 01210
}
} Diane,
} Yes, hot fuming sulfuric and/or hot fuming nitric are used as standard
} procedures for removing plastic from IC's. The process is not quite that
} simple. For example, water rinses will almost certainly etch the bond pads
} on the IC and thus removing connection to the outside world. Additionally,
} the plastic contains fire retardants which some regions don't like being
} washed down the drain. There is more detailed help through EDFAS.org (one
} of ASM's branches). B&G International sells a very safe, effective etcher
} which performs decapsulation automatically in minutes.
}
} I have no association with B&G International.
}
} David Saxon
} Analytical Microscope Services
} 11826 Reservoir Rd. E.
} Puyallup, WA 98374
} 253-848-7701 voice & fax
} email: info-at-analyticalmicroscope.com
} website: www.analyticalmicroscope.com





From daemon Fri Jun 16 08:24:57 2000



From: Steve Chapman :      protrain-at-emcourses.com
Date: Fri, 16 Jun 2000 10:33:15 +0100
Subject: Fractures in the SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi

We often work with clients who wish to look inside materials. Firstly the
SEM is very clever it will tell you if a material is cut with a blade or a
knife or scissors!

The only way to see the true internal structure of a material is to fracture
it. Drop the material into LN2 wait until the bubbles stop and then take it
out and using heavy duty tweezers crack it.

If a material (like hair and some polymer fibres) will not crack you need to
support them in some way to make them crack. We use a water based carbon
solution and two SEM stubs. Glue the two stubs together with the water
soluble adhesive (try Spi) and then drill two or three small holes through
the stubs (about 1mm diameter). Glue the hairs together with the carbon
solution and pass then through the holes (messy). When all is dry plunge
into LN2. Tap a blade between the two stubs and ALL the material should
fracture.

Alternatively, take a fine bore drinking straw and pass the hairs plus
carbon solution into the straw. Wait until dry, dump in LN2 and flex the
straw to crack it and its contents.

Such fractures of layered materials (e.g. paints) will be best viewed in BSE
each "phase" will either be of a different contrast or fracture in a
different way. Great fun, try it?

Steve Chapman
Senior Consultant Protrain
For consultancy and professional training in EM world wide
Tel 44+ 1280 814774 Fax 814007
www.emcourses.com



From daemon Fri Jun 16 08:24:58 2000



From: veys-at-bota.ucl.ac.be (Pascal Veys)
Date: Fri, 16 Jun 2000 10:50:06 +0100
Subject: search for Dr. ND Hallam

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,
I want to contact by Email Prof. N.D. Hallam (formerly at Melbourne and LA
Tobe - Australia)
Does anybody have his contact adress
Thanks to all in advance
Pascal

""""""""'
( O)(o )
--------------------0000--------------0000----------
Pascal VEYS
Laboratory of Plant Biology
Catholic University of Louvain
Place Croix du Sud 5 (bte 14)
B 1348 Louvain-la-Neuve
Belgium
Phone : 0032 10473004
Fax : 0032 10473471
Email : Veys-at-bota.ucl.ac.be
---------------------ooooO-----------Ooooo--------
( ) (_)(_) ( )
) ( ) (
(_) (_)




From daemon Fri Jun 16 08:24:59 2000



From: A.P. Alves de Matos :      apmatos-at-ip.pt
Date: Fri, 16 Jun 2000 11:44:07 +0100
Subject: Saphire Knifes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear All


I«m thinking in buying a saphire knife for ultramicrotomy since they seem to
be less expensive than the traditional diamond ones. I need to cut thin
sections of sponges that are difficul to cut with glass knifes. Does anyone
have experience with these knifes? How do they compare with diamond in terms
of cutting properties and durability?


Thanks

Dr. A.P. Alves de Matos
Dental Medical School
Lisbon



From daemon Fri Jun 16 08:25:01 2000



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Fri, 16 Jun 2000 08:17:39 -0500
Subject: Embedding oocytes in araldite

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Many of our customers are using Agfa Duoscan scanners with excellent
results. These are "flatbed" type scanners but handle films in a separate
drawer, similar to a negative carrier in an enlarger. The advantage of this
system is not scanning through glass, eliminating the chance of Newton
Rings.
In addition, the Duoscan line offers high optical resolutions(up to
2500x2500ppi)and high dynamic ranges.
The Umax Powerlook III is also an excellent scanner where budgets may be
limited. It has 1200x2400 optical resolution and is a traditional "flatbed"
design. Also new is the Linocolor 1400 with 1200x2400 resolution with a
letter size scan bed.

Choosing a printer is more difficult, depending on your output needs. High
end photographic printers such as the Fuji Pictrography or dyesub printers
from Kodak and Sony offer top quality output but at a high price for both
hardware and cost per print. For publication quality prints, these are the
best.
Ink jet printers continue to improve in image quality, and more
importantly, long term image stability. The cost of these printers is very
low although they are very slow, and still somewhat costly per print when
used with the higher quality print materials. Most inkjets are also better
at producing color prints than monochrome prints.
Another favorite of ours is the Tektronix Phaser 850. This high quality
plain paper printer uses a unique Solid Ink technology. Ink is supplied not
in a liquid form but a solid blocks. Cost per print is very low and black
ink is free for the life of the printer.
The Phaser 850 will also handle any "office" type output such as letters
and reports with the advantage of integrating images into pages instead of
attaching all photos at the end.

George Laing
National Graphic Supply


-----Original Message-----
} From: tbonner [mailto:tbonner-at-brockport.edu]


Carrie,

We are currently working on a project involving blastocysts and since we
aren't osmicating the samples, we also have the problem of seeing them in
the resin. Embedding them in agar helps somewhat. Even though it's also
relatively transparent, the larger size of the agar chunk makes it easier to
see.

We perform our primary fixation, then buffer washes, then make a 2% agar
solution on the hot plate. When the agar cools down enough to be quite warm
to the touch (but before the gelling stage), we pipette our cells into it on
a microscope slide or cover slip, then put it into the fridge to harden. It
hardens almost immediately. Then we cut the piece of agar with the sample
into a tiny cube and continue processing it normally.

Hope this helps.

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/




-----Original Message-----
} From: Carrie Golash [mailto:cdg126-at-psu.edu]
Sent: Thursday, June 15, 2000 9:14 PM
To: Microscopy-at-sparc5.microscopy.com



Hello all -

I'm looking for advice on embedding bovine oocytes (~100 micron
diameter).
I'd like to embed them in araldite for probing with labelled lectins as
this seems to be fairly well established in the literature. The hangup is
this: we are using fairly large plastic cassettes and are having problems
losing the oocytes within the volume of araldite. Does anyone know of a way
around this? Is there something we can pre-embed the oocytes in to make a
smaller chip that we can then embed in the larger block (that is, of
course, compatible with polymerizing / clearing the araldite? Or does
anyone know of an altogether different method for oocyte embedding that is
more effective? Thanks in advance!

--Carrie Golash

Carrie Golash
John O. Almquist Research Center
Penn State University
University Park, PA 16802
W: (814) 865-5896
H: (814) 692-7926
http://www.das.psu.edu/dbrc/dbrc.htm




From daemon Fri Jun 16 08:35:23 2000



From: Leona Cohen-Gould :      lcgould-at-mail.med.cornell.edu
Date: Fri, 16 Jun 2000 08:38:15 -0400
Subject: Re: Saphire Knifes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 11:44 AM +0100 6/16/0, A.P. Alves de Matos wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

*********************
I had bought a saphire knife, years ago (early 1980's). Our lab bought it
with the idea that it was a good half-way stop for a new tech who needed
something better than glass. It had certain drawbacks....the edge seemed
to collect debris and was more difficult to clean than a diamond, and of
course it wore faster too. she used it for a while (6 months?) and then
we were able to buy another diamond knife.

I haven't tried a saphire knife since, although I am partial to them in
jewelry!

Lee

Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175




From daemon Fri Jun 16 17:59:08 2000



From: Greg Erdos :      gwe-at-biotech.ufl.edu
Date: Fri, 16 Jun 2000 09:58:04 -0400
Subject: Re: Embedding oocytes in araldite

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


When we were working with porcine oocytes it was necessary to
handle each individually so we enrobed them in agarose inside a cell of
nylon net of a dark color, using a dissecting microscope. We saved enough
of the excess nylon net to use as a "handle" to pick up the sample and
moved it from solution to solution. This should be done after fixation,
since glut fixed agarose is sometimes a problem. We also used the low temp
gelling agarose, so that we had time to work. Then put it in the frig to
solidify. It will then remain solid at room temp.
You might get better lectin labeling using one of the acrylic
resins rather than an epoxy

At 09:13 PM 06/15/2000 -0500, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Gregory W. Erdos, Ph.D.
Assistant Director, Biotechnology Program
PO Box 110580
University of Florida
Gainesville, FL 32611

Ph. 352-392-1295 Fax: 352-846-0251



From daemon Fri Jun 16 17:59:09 2000



From: Alan Bright :      bright-at-dial.pipex.com
Date: Fri, 16 Jun 2000 15:00:37 +0100
Subject: SEM, How to interpret a fractured adhesive film

Contents Retrieved from Microscopy Listserver Archives
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Dear Ric,

I see no reason why this type of cross sectional view cannot be achieved by
sectioning with a cryostat, this is the type of use our cryostats are
supplied for. If you would like more information please get back to me, I
would be happy to section some samples for you to inspect.

Best Regards

Alan Bright

Bright Instrument Co.Ltd.
St Margaret's Way
Huntingdon
PE18 6EB
England

Tel No:+44 (0)1480 454528
Fax No:+44 (0)1480 456031
Email: AlanBright-at-brightinstruments.com
Web Site: www.brightinstruments.com

++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++=

-----Original Message-----
} From: Smartech [mailto:smartech-at-javanet.com]
Sent: 15 June 2000 15:03
To: To all on the list


I needed to have a cross sectional view into an adhesive layer so that I
could count the layers, if possible. Since the adhesive acts like a highly
viscous liquid, polishing it is out of the question. Instead, I submerged
the film into liquid Nitrogen and cut it quickly w/ a scissors. The sound
of the cut was more like a breaking sound. When I examined the cross
sectional surface I observed a brain like surface. There were columns with
a highly consistent diameter and orientation. It looked crystalline. There
was zero evidence of material smearing that one would expect if one cut a
material. Is the proper interpretation that these columns existed before
the fracture and that the fracture occurred along the boundaries? Or is
the cross sectional surface generated by a rippled distortion of an highly
viscous liquid? The regularity of the surface seems to make the latter
interpretation unlikely.

I would be interested in any opinions on the interpretation of the image or
alternative means of cross sectioning the adhesive layer.

I could e-mail an image to anyone who is interested.

Thanks

Ric




From daemon Fri Jun 16 17:59:10 2000



From: Bruce Brinson :      brinson-at-cnst.rice.edu
Date: Fri, 16 Jun 2000 09:35:03 -0500
Subject: Re: Micrographs from digital

Contents Retrieved from Microscopy Listserver Archives
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I'll ring in & say yes. I am quite happy with this combination. You get the
digitized images with a much large field of view. Photo quality ink jets are cheap
& in general do well. You will always find extremist in on the subjects of the
infinitely best scanner & printer but here is what I bought for {9K$.
AGFA Duoscan T2500 ~$4500
500MHz PC with 1/2 Gig memory & 19" hi res monitor, CD writer ~2.5K$
Epson Stylus 870 ~$300
Photo Shop, Fovea 1.0 IP software {1K$ with student ver. of PS
Misc. supplies some $

For a MacPerson, my understanding is that in terms of image processing speed
the Macs are 2-4x faster that PC but I don't have any benchmarks on the latest
generations.

If your printing a lot, the ink jets will drain cartridges pretty quick. Down
the road I will probably pick up one of the wax printers. They cost something like
3k$ but I think it is Tektronix that offers to supply all the black wax you can
user for life, they are pretty fast & no secret papers are required (much cheap
per BW page).

Just my thoughts before coffee.

Bruce Brinson

disclaimer... no financial interest in any companies mentioned.

tbonner wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Being unable to afford a digital camera for my TEM. I'm wondering if the next
} best option is to get a high end scanner to scan in negatives and then print
} them on a decent printer. Any advice regarding this idea and brands of
} scanners and printers that are useful? Also, what image analysis systems are
} user friendly?
}
} Dr. Thomas P. Bonner
} Department of Biological Sciences
} SUNY at Brockport
} Brockport, NY 14420



From daemon Fri Jun 16 17:59:10 2000



From: kklos-at-mail.mse.ufl.edu
Date: Fri, 16 Jun 2000 10:36:03 -0400
Subject: Stuers Jet Polisher

Contents Retrieved from Microscopy Listserver Archives
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I have been trying to replace the photocell on the Jetpolisher that I have
been using. It is about 15 years old and is made by Struers. The Model is
a Tenupol and the power supply is type is Polipower. Struers no longer
makes replacement parts for these units. If anyone has any information
concerning the photocells of this model (who I might contact to replace it
or the sensitivity of the photocell) it would be greatly appreciated.
Regards
Kevin



From daemon Fri Jun 16 17:59:11 2000



From: =shAf= :      mshaf-at-darkwing.uoregon.edu
Date: Fri, 16 Jun 2000 07:37:38 -0700
Subject: RE: Micrographs from digital

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thomas writes ...

} Being unable to afford a digital camera for my TEM. I'm
} wondering if the next best option is to get a high end
} scanner to scan in negatives and then print
} them on a decent printer. ...

The next best option would be a 4x5 film scanner (~US$4k). The
problem with typical film scanners is their anticipated dynamic range
for photographic film, which is where TEM digital capture excels. I
suggest you take a representative film and evaluate the Polaroid "4x5
Ultra". Althought I'm unfamiliar with this particular 4x5 scanner, it
is the only one (I'm aware of) which is purported to scan an OD better
than 3.5 (approximately 14 f/stops ... 4 f/stops per OD unit ...
correct me if I'm wrong).
Less expensive (~US$1.2k) would be a flat bed scanner designed for
transparencies as well as hardcopy. This additional feature could be
a "drawer" for film, or a optional "lid" which provides a lamp from
above.

=shAf=



From daemon Fri Jun 16 17:59:13 2000



From: EBMet-at-aol.com
Date: Fri, 16 Jun 2000 11:34:47 EDT
Subject: re: Cross-section Preparation of Magnetic Recording Tape

Contents Retrieved from Microscopy Listserver Archives
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To All:

I need tips, descriptions, references regarding the preparation of
cross-section specimens of the magnetic layer of magnetic recording tape for
high magnfication SEM and conventional TEM observation. Thanks.

Elliot Brown
EBMET-at-AOL.COM



From daemon Fri Jun 16 17:59:14 2000



From: Bill Miller :      microbill-at-mohawk.net
Date: Fri, 16 Jun 2000 11:48:57 -0400
Subject: RE: Micrographs from digital

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Actually the printing part has recently gotten much
easier. ElectroImage (http://www.electroimage.com) is offering new
technology that lets you print real grey scale images on simple inkjet
printers. They have grey inks and new printer drivers.

Bill Miller



At 08:47 AM 6/16/00 -0700, George Laing wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Fri Jun 16 17:59:15 2000



From: David Henriks :      Henriks-at-CompuServe.COM
Date: Fri, 16 Jun 2000 12:11:08 -0400
Subject: re: Cross-section Preparation of Magnetic Recording Tape

Contents Retrieved from Microscopy Listserver Archives
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Elliot:

Off hand I know I have information on the cross sectioning of hard disk
media using the Tripod Polisher¨. I'm not sure if I have anything on
magnetic recording tape. If you send me your mailing address, I'll send
you whatever I can find that comes close.

Best regards-

David
Writing at 8:46:59 AM on 06/16/2000

***************************************************************************
************************

David Henriks
Vice President TEL: 800-728-2233
(toll free in the USA)
South Bay Technology, Inc. +1-949-492-2600
1120 Via Callejon FAX: +1-949-492-1499
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

***************************************************************************
************************

} } } } } Please visit us at http://www.southbaytech.com { { { { {

Manufacturers of precision sample preparation equipment and supplies for
metallography, crystallography and electron microscopy.

Message text written by INTERNET:"EBMet-at-aol.com"-at-sparc5.microscopy.com
}
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


To All:

I need tips, descriptions, references regarding the preparation of
cross-section specimens of the magnetic layer of magnetic recording tape
for
high magnfication SEM and conventional TEM observation. Thanks.

Elliot Brown
EBMET-at-AOL.COM

{



From daemon Fri Jun 16 17:59:15 2000



From: Edward Haller :      ehaller-at-hsc.usf.edu
Date: Fri, 16 Jun 2000 12:24:30 -0400
Subject: Osmium fix

Contents Retrieved from Microscopy Listserver Archives
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In response to Eric's question, I run a diagnostic Pathology lab at the
University of South Florida, and have a one day processing schedule for
kidney biopsies that calls for osmication in 1% buffered osmium at room
temperature for 30 minutes. I have found that for tissue pieces in the
order of 1/2 millimeter thick in one dimension the fixation is fine, and
is equivalent to our routine processing osmium fixation of one hour at 4
degrees. I haven't tried this on muscle biopsies, but if they meet the
thickness criteria they should be O.K. too,. Just make sure to rinse
these extensively (3x 10 minutes, perhaps) in buffer to remove the
excess osmium from the muscle tissue, as fluids enter and leave muscle
slower because of the extensive connective tissue sheaths around the myocytes.
Overosmication is a definite possibility, with subsequent tissue
brittleness, if tissue is left in osmium too long, no matter what the
temperature. If the tissue is too thick, uneven osmication can occur,
where the outside of the tissue is well fixed and a fixation gradient is
set up with poor fixation towards the center of the tissue. I observed
this happening at a renal lab in Pittsburgh where I used to work. Our
unstained thick sections were darker at the periphery than in the
center. Another sign of this problem is lack of specimen contrast at the
center of thin sections.
So, Eric, as far as my experience goes, it is possible to start with 4
degree, buffered osmium, and to osmicate at room temperature for a half
of an hour and get results equal to those from osmication at 4 degrees
for one hour if your tissue is sufficiently thin in at least one
dimension. I haven't noticed any precipitation problems with this
technique, nor does the osmium discolor during fixation. Our lab has
been using this technique for several years now. Take care! Ed Haller,
U.S.F. Pathology



From daemon Fri Jun 16 17:59:16 2000



From: David Henriks :      Henriks-at-CompuServe.COM
Date: Fri, 16 Jun 2000 12:33:25 -0400
Subject: Struers Jet Polisher

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Kevin:

You may want to ask Struers for a users list. There may be someone out
there with an older unit that is no longer being used who may be willing to
give it to you for spare parts. If they can't give you a list, let me know
- I think I can dig up an old list I put together of some previous Tenupol
users you may be able to contact. If that doesn't work, you may want to
consider upgrading to a South Bay Technology Model 550D Jet Polisher. If
you have an interest in getting more information on that option, please
contact me and I'll send you information.

Best regards-

David
Writing at 8:56:57 AM on 06/16/2000

***************************************************************************
************************

David Henriks
Vice President TEL: 800-728-2233
(toll free in the USA)
South Bay Technology, Inc. +1-949-492-2600
1120 Via Callejon FAX: +1-949-492-1499
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

***************************************************************************
************************

} } } } } Please visit us at http://www.southbaytech.com { { { { {

Manufacturers of precision sample preparation equipment and supplies for
metallography, crystallography and electron microscopy.

Message text written by
INTERNET:"kklos-at-mail.mse.ufl.edu"-at-sparc5.microscopy.com
}
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America



I have been trying to replace the photocell on the Jetpolisher that I have
been using. It is about 15 years old and is made by Struers. The Model is
a Tenupol and the power supply is type is Polipower. Struers no longer
makes replacement parts for these units. If anyone has any information
concerning the photocells of this model (who I might contact to replace it
or the sensitivity of the photocell) it would be greatly appreciated.
Regards
Kevin
{



From daemon Fri Jun 16 17:59:16 2000



From: Edward Haller :      ehaller-at-hsc.usf.edu
Date: Fri, 16 Jun 2000 12:58:34 -0400
Subject: Immunofluorescence using plastic coverslips

Contents Retrieved from Microscopy Listserver Archives
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To the person asking about the possibility of doing immunofluorescence
microscopy on cells grown on plastic coverslips, someone has published a
technique in BioTechniques that I saved in case I needed it. Volume24,
number 6, pages 910-914, 1998 is the article titled "Mounting technique
allows observation of immuno-labeled cells on plastic coverslips".
The basic technique from M. F. Donohue et al involves using Thermanox
coverslips on which cells are grown and immunolabeled. Following
labeling, this group uses a drop of aqueous mounting medium to mount the
side of the coverslip without cells on it to a glass slide. On top of
this, the group then mounted a regular glass coverslip with an
additional drop of aqueous mountant, and then could do their microscopy.
The authors state that the inherent strong autofluorescence is greatly
reduced by this technique, and the problem with the plastic not
transmitting light well is overcome. Although I haven't tried the
technique yet, it sounds like a simple fix for a sticky problem. I hope
this is of help to you! Ed Haller, U.S.F. Pathology



From daemon Fri Jun 16 17:59:17 2000



From: Tom Januszewski :      tom.januszewski-at-email.swmed.edu
Date: Fri, 16 Jun 2000 11:50:10 -0500
Subject: Tissue Processors

Contents Retrieved from Microscopy Listserver Archives
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Hi,
We are currently in the market for a tissue processor for electron
microscopy. It will mainly be used for biolgical specimens (some quite
small). Although I have considerable experience with the processor sold
by RMC (Ventana), I know virtually nothing about the Lynx (now being
sold by EMS, I think). Any information on the advantages or
disadvantages of either model (or any other one that might be out there)
would be appreciated. Offline replies are welcome.

Tom Januszewski
Senior Electron Microscopist
Molecular and Cellular Imaging Facility
UT Southwestern Medical Center at Dallas
5323 Harry Hines Blvd.
Dallas, TX 75390
Email: tom.januszewski-at-email.swmed.edu



From daemon Fri Jun 16 17:59:17 2000



From: Glen MacDonald :      glenmac-at-u.washington.edu
Date: Fri, 16 Jun 2000 10:22:28 +0100
Subject: Re: Micrographs from digital

Contents Retrieved from Microscopy Listserver Archives
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We are using the Agfa T2500 to scan TEM and SEM negatives, which also
gives us the ability to scan prints. The 1200 dpi is sufficient for
most needs, with 2500 dpi getting used less often and mostly with low
mag images. It is currently connected to a 233 MHz Mac G3/160 Mb RAM,
being replaced with a 400 MHz G4/320 Mb. The Agfa is driven by either a
stand alone app. or through a plug-in that runs under most software,
such as Photoshop or Object Image. You will want a lot RAM and drive
space, CDR, Ord, Jaz or DVD-RAM drives. Zip drives fill far too quickly.


Photoshop is used for publication images, although some users prefer
Canvas. Most of our image analysis uses the Object Image enhanced
version of NIH Image. It is very easy to use. I've less experience with
Image/J, but it is quickly adding capabilities and will display } 8 bits,
whereas Object Image will process 16 but only displays 8 bits. Printing
is either to an Epson 850, 3000, or our venerable Phaser IIsdx. BTW,
Tektronix sold its printer division to Xerox.

tbonner wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Being unable to afford a digital camera for my TEM. I'm wondering if the next
} best option is to get a high end scanner to scan in negatives and then print
} them on a decent printer. Any advice regarding this idea and brands of
} scanners and printers that are useful? Also, what image analysis systems are
} user friendly?
}
} Dr. Thomas P. Bonner
} Department of Biological Sciences
} SUNY at Brockport
} Brockport, NY 14420

--

Glen MacDonald
Virginia Merrill Bloedel Hearing Research Center
Box 357923
University of Washington
Seattle, WA 98195-7923
glenmac-at-u.washington.edu
(206) 616-4156
(206) 616-1828 fax
***********************************************************
C:} The box said "Requires Windows95 or better". So I bought a Macintosh.
***********************************************************



From daemon Fri Jun 16 17:59:21 2000



From: Caroline Schooley :      schooley-at-mcn.org
Date: Fri, 16 Jun 2000 11:47:54 -0700
Subject: Re: Saphire Knifes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} I«m thinking in buying a saphire knife for ultramicrotomy since they seem to
} be less expensive than the traditional diamond ones. I need to cut thin
} sections of sponges that are difficul to cut with glass knifes. Does anyone
} have experience with these knifes? How do they compare with diamond in terms
} of cutting properties and durability?
}
} Dr. A.P. Alves de Matos
} Dental Medical School
} Lisbon

Unfortunately, you get what you pay for. Sapphire does NOT have the
durability of diamond and will be damaged by the sponge spicules. And, to
my knowldge, they can't be resharpened.


Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html




From daemon Fri Jun 16 17:59:24 2000



From: Jon Ekman :      jekman-at-csd.uwm.edu
Date: Fri, 16 Jun 2000 03:18:56 -0500
Subject: Re: Micrographs from digital

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


It could be a solution if you mainly want an alternative to silver prints,
or to acquire images for Powerpoint presentations. However, many
of the really useful features of a digital camera on a TEM are
unavailable if you adopt this approach, namely instant verification of
image capture, greyscale expansion, image averaging, online
analysis, and many more. Also, you still need to allocate some
space to a darkroom.

I looked at large-format transparency scanners a couple of years
ago, when there was little of this kind in the market, and concluded
that their combinations of bit depth, pixels per inch and sensitivity
and dynamic range at the high-density end of the negative (i.e.
highlight detail) was close to what was required if the objective was
merely to obtain publication quality images from a large proportion
of negative area (these images require optimised contrast and
crispness, but being small do not demand much resolution), but
really inadequate for scanning of image details (organelles,
molecules), for dense exposures and highlights, and for high
contrast subjects like replicas. Most of these scanners appeared to
be optimised for scanning positive images (large format colour
transparencies) where discrimination of detail in the extreme
shadows is not top priority. However, this becomes a major
shortcoming when dealing with negatives.

I would certainly like to know whether anyone feels that there is an
adequate solution available today.

I don't think user friendliness is the most useful criterion for
discriminating between image analysis packages. This is in any
case a fairly subjective property, depending very considerably on
the computer - literacy of the user. Most IA packages (analySIS,
Optimas, etc) are GUI-based systems, and therefore are reasonably
intuitive. One of the features that differentiates them is the balance
between the provision of off-the-peg analysis solutions and
programmability. The range of tasks demanded of an IA package is
potentially so great that there is little alternative but to evaluate them
and see if they suit your needs. However, I warn you that your
needs are likely to evolve. What seems like a simple and user-
friendly solution today will probably feel like a very limited and
inflexible one tomorrow if it has insufficient functionality and
programmability, and these things unavoidably add complexity.

Chris Jeffree

Date sent: Thu, 15 Jun 2000 21:14:18 -0500
To: Microscopy-at-sparc5.microscopy.com
} From: tbonner {tbonner-at-brockport.edu}



tbonner said:
} Being unable to afford a digital camera for my TEM. I'm wondering
} if the next best option is to get a high end scanner to scan in
} negatives and then print them on a decent printer.

Using a flat bed scanner over a digital camera for TEM images is
definately your best option budget wise. We use an Agfa Duoscan
scanner with great success. it is very versitile, we use it to scan
gels, scan TEM negatives and scan old prints. It can scan slides for
powerpoint quality presentations also. So check with Agfa to see
their latest lineup.

For printers inkjets work great when combined with photoquality
papers. Any (Epson HP & Cannon) 300dpi or higher color ink jet will
give decent images suitable for posters. For publications dye
sublimation printers work well but get one that uses a cartridge (one
piece) to replace empty media. For proofing look for laser printers
that are at least 1200dpi with extra ram (64meg on the printer is a
nice number to start with) and large toner cartridges, graphic
images burn a lot of toner. Look for a printer that will print alot
before replacement of the toner.

For software, Photoshop is a good choice. Before you spend alot on
a venders image analysis package try some of the freeware out
there like NIH image and Image J both from the NIH website. If you
can't get the free stuff to work, then spend the extra money. We use
Image J here and it works well.

For computers (Apple or PC) consider one scanner with a SCSI
interface with need a SCSI card (avoid parallel port and universal
serial bus (USB) scanners unless you like coffee breaks). So you
need one computer to hook up to the scanner but Ideally you would
also have three printers (inkjet, Laser & Dye sub or comparable) But
Inkjets are cheap make your users get one and maintain it. I bet
somewhere in your department is a networked laser printer so print
to a networked printer elsewhere. Keep the high end printer (Dye
sub/thermal printer close by) Invest in removable media that others
can use like a CD-r (HP or Plextor) and a zip drive at the bare
minimum.


Hope this helps
Jon Ekman
Associate Research Specialist
Deptartment of Biological Sciences
University of Wisconsin-Milwaukee
phone W:414.229.6471
Web1 http://www.graffitimasters.com
Web2 http://www.uwm.edu/~jekman



From daemon Fri Jun 16 18:19:28 2000



From: Malis, Tom :      malis-at-nrcan.gc.ca
Date: Fri, 16 Jun 2000 15:48:24 -0400
Subject: RE: Cross-section Preparation of Magnetic Recording Tape

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Embed it, Elliot and diamond knife section it in an ultramicrotome, using
the block face for the FE-SEM examination and the ultrathin sections (20-200
nm thick) for examination in the TEM. A good reference is Ho et al,
Specimen Preparation for TEM of Materials, Mat. Res. Soc. Symp. Proc., vol.
115, pp. 149-154 (MRS, Pittsburgh, 1988). You may have to search around for
someone who can do this for you, but it is far and away the best technique
for your needs.

Tom Malis
Group Leader - Characterization
Materials Technology Laboratory
Natural Resources Canada (Govt. of Canada)
568 Booth St., Ottawa, Canada
ph. 613-992-2310
FAX 613-992-8735
email: malis-at-nrcan.gc.ca


----------
From: "EBMet-at-aol.com"-at-sparc5.microscopy.com
[SMTP:"EBMet-at-aol.com"-at-sparc5.microscopy.com]
Sent: Friday, June 16, 2000 11:35 AM
To: microscopy-at-sparc5.microscopy.com
Subject: re: Cross-section Preparation of Magnetic Recording Tape


------------------------------------------------------------------------
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To All:

I need tips, descriptions, references regarding the preparation of
cross-section specimens of the magnetic layer of magnetic recording
tape for
high magnfication SEM and conventional TEM observation. Thanks.

Elliot Brown
EBMET-at-AOL.COM



From daemon Fri Jun 16 23:04:06 2000



From: Gen Pei :      gp35-at-cornell.edu
Date: Fri, 16 Jun 2000 22:57:08 -0400
Subject: How to know the ion milling rate

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


HI,

I am ion milling gold. nearly 100 nm. I want to know how long it
takes to make it thin enough to be transparent under TEM. Thanks.

Gen
******************************************************************
Gen Pei
Department of Materials Science and Engineering
Cornell University
328 Thurston Hall
tele: (607)255-5177
fax:(607)255-2365
gp35-at-cornell.edu

******************************************************************



From daemon Sat Jun 17 09:24:45 2000



From: cgarber-at-2spi.com
Date: Sat, 17 Jun 2000 06:52:25 -0700
Subject: RE: Re: Saphire Knifes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



For your information:

a) So far as I know they are not made any more and have not been
made for at least ten years, and

b) The economics have changed drastically from twenty years ago
when the sapphire knife did enjoy a bit of popularity. In real
terms, diamond knives, now because of the competition from Microstar
have drop significantly from what they once were, perhaps 50%,
so whatever pricing advantage there was at one time, did not
exist any more. So the Japanese company that made them discontinued
their production. It was called "Saphatome" or something like
that. Ted Pella would probably know their history, perhaps better
than I do.

Also, because of your interest in education, take a look at www.microscopy-advantage.com
. Tell me what you think. Attendees at the coming meetings
of APEM, EUREM and MSA will automatically receive a CD in their
registration materials. If you would like a copy, send me your
UPS address and I will make sure that one gets sent to you. But
it will "work" exactly as if you were on line.
--- Original Message ---
Caroline Schooley {schooley-at-mcn.org} Wrote on
Fri, 16 Jun 2000 11:47:54 -0700
------------------
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society
of America



} I´m thinking in buying a saphire knife for ultramicrotomy since
they seem to
} be less expensive than the traditional diamond ones. I need
to cut thin
} sections of sponges that are difficul to cut with glass knifes.
Does anyone
} have experience with these knifes? How do they compare with
diamond in terms
} of cutting properties and durability?
}
} Dr. A.P. Alves de Matos
} Dental Medical School
} Lisbon

Unfortunately, you get what you pay for. Sapphire does NOT have
the
durability of diamond and will be damaged by the sponge spicules.
And, to
my knowldge, they can't be resharpened.


Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html





-----
Sent using MailStart.com ( http://MailStart.Com/welcome.html )
The FREE way to access your mailbox via any web browser, anywhere!



From daemon Sat Jun 17 10:23:14 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Sat, 17 Jun 2000 08:11:45 -0700
Subject: Re: Micrographs from digital

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 12:15 PM 6/16/00, you wrote:

} It could be a solution if you mainly want an alternative to silver prints,
} or to acquire images for Powerpoint presentations. However, many
} of the really useful features of a digital camera on a TEM are
} unavailable if you adopt this approach, namely instant verification of
} image capture, greyscale expansion, image averaging, online
} analysis, and many more. Also, you still need to allocate some
} space to a darkroom.

Why not just outsource the processing of the film? Depending
on where one resides/operates, there are typically numerous
professional and non-professional labs which will do same day
development of b/w film. I do this for 4x5 cut sheet film and
120/220 roll film from a regular camera and from the SEM recording
camera. Unless there is some overriding need or requirement for
an on-site darkroom, why not just send the film out whenever
it is needed? I could see the rationale for an on-site facility if
the TEM was producing hundreds of negs per day or perhaps
per week. Then it is a make-buy decision regarding in-house
or out-house processing.

If one is concerned about whether a shot will turn out (instant
verification), just shoot a couple more sheets or frames bracketed
around the "optimum/normal" exposure time. The cost of the
film and processing is way too low to justify a high cost digicam
for TEM. SEM imaging is of course a totally different
matter.

I find that grey scale expansion is not the sole domain of the
digicam. In a neg, additional information is there--but typically
the eye cannot see it. This is where image analysis and image
processing programs are very beneficial.


} I looked at large-format transparency scanners a couple of years
} ago, when there was little of this kind in the market, and concluded
} that their combinations of bit depth, pixels per inch and sensitivity
} and dynamic range at the high-density end of the negative (i.e.
} highlight detail) was close to what was required if the objective was
} merely to obtain publication quality images from a large proportion
} of negative area (these images require optimised contrast and
} crispness, but being small do not demand much resolution), but
} really inadequate for scanning of image details (organelles,
} molecules), for dense exposures and highlights, and for high
} contrast subjects like replicas. Most of these scanners appeared to
} be optimised for scanning positive images (large format colour
} transparencies) where discrimination of detail in the extreme
} shadows is not top priority. However, this becomes a major
} shortcoming when dealing with negatives.

Discrimination of detail in shadows is a major concern for
users of transparencies. This is why they seek high D rated
scanners. I typically scan negative and transparencies as
transmitted RGB or greyscale. This is because I find that the
scanner picks up more detail across the whole image when
scanned as a tranny.

} [snip]

} and see if they suit your needs. However, I warn you that your
} needs are likely to evolve. What seems like a simple and user-
} friendly solution today will probably feel like a very limited and
} inflexible one tomorrow if it has insufficient functionality and
} programmability, and these things unavoidably add complexity.
}
} Chris Jeffree

I agree that needs may evolve. That means that when making
the initial purchase of an image analysis program, it should
be flexible enough to allow custom augmentation. Most of
the higher end ones do. But it may turn out that one program
alone is not as good as two different programs--each being
good at different aspects of image analysis.

gary g.


~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Modern surfers use PC boards. You can too at
http://photoweb.net
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~



From daemon Sat Jun 17 10:53:16 2000



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Sat, 17 Jun 2000 11:50:47 -0500
Subject: Magnetic recording tape

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Tom Malis wrote:
======================================================
Embed it, Elliot and diamond knife section it in an
ultramicrotome, using
the block face for the FE-SEM examination and the ultrathin
sections (20-200
nm thick) for examination in the TEM. A good reference is Ho et
al,
Specimen Preparation for TEM of Materials, Mat. Res. Soc. Symp.
Proc., vol.
115, pp. 149-154 (MRS, Pittsburgh, 1988). You may have to search
around for
someone who can do this for you, but it is far and away the best
technique
for your needs.
======================================================

This has been our experience too, however we add the following to the
preparation protocols:

a) We coat one side of the recording tape with, say, Pt, the other side
with Al, because once in the TEM, it is important to validate that i]
nothing has fractured off during the ultramicrotomy and ii] you can keep
straight which side is which for the asymmetric cross-section. If the two
metallization lines are present in the TEM, with embedding resin on the
other side, you can be certain you are seeing the entire cross-section. If
one is missing, you might not have the entire cross-section.

b) For looking at the "faced-off-piece", we suggest an ever so slight
amount of oxygen plasma etching, in order to bring out a bit more contrast
between the ferrite or other inorganics from the matrix polymer. The
inorganics stand up like little "mesas" in the desert, giving greatly
enhanced contrast. Since you now have an element of three dimensional
nature to the same, you can gain some insight into orientation, something
that would not otherwise be possible

Disclaimer: If you are looking for someone to do this kind of work, look no
further, we have been doing this kind of sample preparation for clients on
a contract basis since the early 1970's! Our own Plasma Prep II plasma
etcher would do the described etching on the faced-off-piece after about 120
seconds of exposure.

Chuck


============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.spi.cc
########################
============================================





From daemon Sat Jun 17 17:33:47 2000



From: Caroline Schooley :      schooley-at-mcn.org
Date: Sat, 17 Jun 2000 09:10:57 -0700
Subject: Re: Micrographs from digital

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

And if you're satisfied with prints at 720x1440 dpi, Epson has just made a
major leap in ink and paper longevity; read about it at
http://www.epson.com/whatsnew/ygtsi/lightfast.html
http://www.wilhelm-research.com/ . Unfortunately, the new ink cartridge
won't fit old (as in last year's) printers. Oh well - what else might I
spend $370 on?

Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html




From daemon Sat Jun 17 17:33:52 2000



From: Dave Audette :      deaudette-at-yahoo.com
Date: Sat, 17 Jun 2000 13:57:45 -0700 (PDT)
Subject: job posting

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


List Recipients:
I am posting this message at the request of Joe Lester
and all correspondence should be sent to him.
Dave Audette
david.audette-at-sylvania.com

JOB OPENING
Scanning Electron Microscopist in Analytical
Laboratory ( Beverly, MA)

OSRAM Sylvania, Inc.
71 Cherry Hill Drive
Beverly MA 01915

DESCRIPTION:
Structural and elemental characterization of
materials used in incandescent,
fluorescent and discharge lamps, especially by
optical and electron
microscopy. Failure analyses of lamps and lighting
components. Technical
problem solving as a member of a team. Oral and
written communication of
results and conclusions with client population.

POSITION REQUIREMENTS:
Competence in optical and electron microscopy of
materials including EDS. An
understanding of failure analysis. Ability to work
independently and/or in a
team and to communicate effectively.

EDUCATION AND EXPERIENCE REQUIREMENTS:
B.S., or higher, in Materials Science,
Chemistry, or Physics. 2-5
years experience in SEM/EDS. Experience with lamp
components is desirable.


Please send a resume to


Dr. Joe Lester
Technical Assistance Lab
OSRAM Sylvania Inc.
71 Cherry Hill Drive
Beverly, MA 01915
e-mail: joe.lester-at-sylvania.com



__________________________________________________
Do You Yahoo!?
Send instant messages with Yahoo! Messenger.
http://im.yahoo.com/



From daemon Sun Jun 18 19:19:45 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Sun, 18 Jun 2000 19:04:44 -0500
Subject: Administrivia: Listserver Archives Updated.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Colleagues....

I had fallen behind on the Archive Updates. They are now
current through May 31, 2000.

http://www.msa.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Nestor
Your Friendly Neighborhood SysOp.





From daemon Sun Jun 18 19:29:41 2000



From: John Nailon :      mmjnailo-at-dingo.cc.uq.edu.au
Date: Mon, 19 Jun 2000 10:23:25 +1000
Subject: Re: Saphire Knifes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


G'day All,
In my experience Sapphire Knives are an excellent replacement for glass
knives when working with soft materials. Sapphires are much softer than
diamond and are more easily damaged than diamond. Sponges are NOT soft
tissue, they contain very hard inorganic salt spicules that damage both
glass and sapphire knives.
Regards
JVN

Leona Cohen-Gould wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} At 11:44 AM +0100 6/16/0, A.P. Alves de Matos wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Dear All
} }
} }
} } I«m thinking in buying a saphire knife for ultramicrotomy since they seem to
} } be less expensive than the traditional diamond ones. I need to cut thin
} } sections of sponges that are difficul to cut with glass knifes. Does anyone
} } have experience with these knifes? How do they compare with diamond in terms
} } of cutting properties and durability?
} }
} }
} } Thanks
} }
} } Dr. A.P. Alves de Matos
} } Dental Medical School
} } Lisbon
}
} *********************
} I had bought a saphire knife, years ago (early 1980's). Our lab bought it
} with the idea that it was a good half-way stop for a new tech who needed
} something better than glass. It had certain drawbacks....the edge seemed
} to collect debris and was more difficult to clean than a diamond, and of
} course it wore faster too. she used it for a while (6 months?) and then
} we were able to buy another diamond knife.
}
} I haven't tried a saphire knife since, although I am partial to them in
} jewelry!
}
} Lee
}
} Leona Cohen-Gould, M.S.
} Sr. Staff Associate
} Director, Electron Microscopy Core Facility
} Manager, Optical Microscopy Core Facility
} Joan & Sanford I. Weill Medical College
} of Cornell University
} voice (212)746-6146
} fax (212)746-8175

--
****************************************************
John V Nailon
Operations Manager
Centre for Microscopy and Microanalysis
The University of Queensland
St. Lucia Queensland 4072
Phone: +61-7-3365-4214
Fax: +61-7-3365-4422
WWW: http://www.uq.edu.au/nanoworld/allstaff.html#Nailon
****************************************************



From daemon Sun Jun 18 21:29:21 2000



From: XUELI ZHAO :      xzhao-at-unlserve.unl.edu
Date: Sun, 18 Jun 2000 21:10:49 -0500 (CDT)
Subject: Sight seeing while EM meeting

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi, Every one:

I had a argument with my husband, he says he is going to the
Kunming for an EM meeting,

http://www.iphy.ac.cn/microsc/IKSM.html

He says there are many good scientists going which I believe with doubt.
But I think he is going for a sight seeing. There is a Chinese saying:
The mountains and waters in Guilin are the most beautiful ones under the
sky. I have asked him to bring me, he agreed but unable to get the same
airline ticket (UA fully booked). Any body is going and knows alternative
airlines, please contact me. Thanks a lot.

Xueli




From daemon Mon Jun 19 07:37:44 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Sun, 18 Jun 2000 20:55:08 -0700
Subject: Warning about DVD-RAM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have two DVD-RAM drives and 15 media (Type I and II).
I have found that these are riddled with write & read errors.
Be careful when using this storage media.

My main unit is a Panasonic LF-D101 (SCSI) and
the second one is the same. The third is a Matshushita
ID unit.

Scandisk will report either many errors that are fixed or
no errors. Either way, the media/drive will write faulty
file contents.

Be careful.




From daemon Mon Jun 19 10:01:27 2000



From: ComCryLab1-at-aol.com
Date: Mon, 19 Jun 2000 10:27:57 EDT
Subject: CRYSTAL: MgAl2O4

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Hao,
We have in stock (100), (110) and (111) MgAl2O4 substrates with 7 angstrom
finish ready for laser ablation or other kinds of epitaxial film deposition.
Contact me for info regarding perovskite lattice matching.
Best regards,
Mike Urbanik
www.crystalguru.com

{ { Subj: information about MgAl2O4
Date: 6/15/00 3:33:44 PM Eastern Daylight Time
From: haoli-at-glue.umd.edu (Hao Li)
To: Microscopy-at-sparc5.microscopy.com

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America
To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
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-----------------------------------------------------------------------.


Hi, All:

This one is not really related to microscopy. But since I am a TEM guy, I
hope I can get some help here.

Does anybody have some information about MgAl2O4 as a substrate for
perovskite films? I know it is not often used, so I am worndering if it has
a major disadvantage so that nobody is using it. Any references would be
welcome.


Thanks a lot

Hao Li

} }



From daemon Mon Jun 19 10:01:28 2000



From: Leona Cohen-Gould :      lcgould-at-mail.med.cornell.edu
Date: Mon, 19 Jun 2000 09:46:49 -0400
Subject: TEM of yeast

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All,
I processed a series of pellets of yeast for a client using a glut-pfa fix,
osmium, dehydration through ethanols, and a day & a half step-wise
infiltration into Spurr's resin (1:1 with Ethanol for 4 hr, pure resin
overnight under light vacuum, then fresh for 3 more hours, then embed in
fresh). Polymerization was overnight at 60C. About half the blocks were
soft and had to be returned to the oven for a prolonged polym. (over the
weekend). I was able to get sections from each of the 10 samples, but in
the 'scope, many of these looked a bit like swiss cheese. those that were
not lacy exhibited areas where the resin pulled away from the cell coats of
the yeasts. Clearly something went wrong with the
infiltration/polymerization. I used the same batch of resin for other
things, and its fine.

Does anyone out there have experience with yeast? Any suggestions?
I'd like to give this peson some usable data!

Thanks in advance,
Lee

Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175




From daemon Mon Jun 19 18:23:25 2000



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Mon, 19 Jun 2000 10:09:46 -0500
Subject: TEM of yeast

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Lee,

My only thought is that your infiltration/dehydration were too short, or
your 100% ethanol had absorbed too much water. My understanding is that
Spurr's is very sensitive to small quantities of water, with soft blocks and
holes being symptoms of incomplete dehydration. Sometimes we extend the
dehydration through 3 changes of 100% ETOH with molecular sieves, then on
through 2-3 changes of propylene oxide. Infiltration is usually 1:2
PO:Resin, followed by 1:1, 2:1, then a couple changes of pure resin
overnight or for 4-8 hours, then final embedding in another change of pure
resin.

I don't know if yeast is more problematic than other things in this respect,
however.

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/




-----Original Message-----
} From: Leona Cohen-Gould [mailto:lcgould-at-mail.med.cornell.edu]
Sent: Monday, June 19, 2000 8:47 AM
To: Microscopy-at-sparc5.microscopy.com


Hi All,
I processed a series of pellets of yeast for a client using a glut-pfa fix,
osmium, dehydration through ethanols, and a day & a half step-wise
infiltration into Spurr's resin (1:1 with Ethanol for 4 hr, pure resin
overnight under light vacuum, then fresh for 3 more hours, then embed in
fresh). Polymerization was overnight at 60C. About half the blocks were
soft and had to be returned to the oven for a prolonged polym. (over the
weekend). I was able to get sections from each of the 10 samples, but in
the 'scope, many of these looked a bit like swiss cheese. those that were
not lacy exhibited areas where the resin pulled away from the cell coats of
the yeasts. Clearly something went wrong with the
infiltration/polymerization. I used the same batch of resin for other
things, and its fine.

Does anyone out there have experience with yeast? Any suggestions?
I'd like to give this peson some usable data!

Thanks in advance,
Lee

Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175




From daemon Mon Jun 19 18:23:27 2000



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Mon, 19 Jun 2000 09:17:18 -0700
Subject: Re: How to know the ion milling rate

Contents Retrieved from Microscopy Listserver Archives
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Dear Gen Pei,
I have looked at gold contacts, lifted from an electronic device, that were
supposed to be 100 nm thick. I could see the structure clearly at 200 kV.
At 10:57 PM 6/16/00 -0400, you wrote:

} HI,
}
} I am ion milling gold. nearly 100 nm. I want to know how long it
} takes to make it thin enough to be transparent under TEM. Thanks.
}
} Gen

Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Mon Jun 19 18:23:29 2000



From: jmkrupp-at-cats.ucsc.edu (Jon Krupp)
Date: Mon, 19 Jun 2000 11:25:24 -0700
Subject: Formvar & 1,2 dichloroethane

Contents Retrieved from Microscopy Listserver Archives
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Hi:

I was just visited by one of our EH&S folks who wanted to know why I had
1,2 dichloroethane.

Seems they track purchases and I bought some last year to make formvar films.

1,2 dichloroethane is on their bad list as a carcinogen. He was actually
here to figure out how much might be going up the fume hood so he could
make a report to the local air quality agency. But as we talked, it seemed
like it would be better to not have the stuff around.

Anyone have experience with formvar in chloroform? I read it works but have
never tried it. According to our EH&S guys, chloroform would be better than
1,2, dichloroethane.

BTW we are in California and must abide by some pretty strict rules, it may
seem like they are going overboard, but they are just trying to do their
job.

Jonathan Krupp
Microscopy & Imaging Lab
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu




From daemon Mon Jun 19 18:23:29 2000



From: Connie A Cummings :      rosscac-at-okstate.edu
Date: Mon, 19 Jun 2000 12:42:17 -0500
Subject: Sperm Processing for TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


{P} Good day to all on the listserver, {/P} {P}   {/P} {P} I have a person in my department who is interested in processing horse sperm for TEM.  Does anyone who does this routinely be willing to give me some pointers as to how to process them?  I've only worked with muscle and brain tissue so this is kinda new - I have processed cells from cell culture for TEM would it be the same procedure? {/P} {P}   {/P} {P} Thanks so much, {/P} {P} Connie Cummings, DVM {/P} {P} Instructor Anatomic Pathology {/P} {P} Department VBP {/P} {P} Oklahoma State University {/P} {P} {/P}

{/html}



From daemon Mon Jun 19 18:23:30 2000



From: Mike Coviello :      coviello-at-mae.uta.edu
Date: Mon, 19 Jun 2000 15:12:30 -0500
Subject: TEM-looking for a for-hire independent FIB company that does TEM

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---------- Forwarded message ----------


Hi Y'all:
We are looking for a for-hire independent FIB company that has
experience in preparing TEM cross-sections of semiconductors. Please
contact me if you do this, or know of a lab that does.
Regards,
Michael Coviello
Lab Manager
Materials Science
University of Texas at Arlington



From daemon Mon Jun 19 18:23:32 2000



From: Leona Cohen-Gould :      lcgould-at-mail.med.cornell.edu
Date: Mon, 19 Jun 2000 16:06:34 -0400
Subject: RE: TEM of yeast

Contents Retrieved from Microscopy Listserver Archives
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Thanks to everyone for your helpful comments. I'm taking another stab at
it with smaller pellets, longer times and the addition of prop. ox. steps
after the ethanol.
I had pretty much decided to do all that anyway, but its nice to gets
confirmation ofone's ideas!

Lee

Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175




From daemon Mon Jun 19 18:23:33 2000



From: Jean-Pierre Slakmon :      slakmon-at-soquelec.com
Date: Mon, 19 Jun 2000 17:56:55 -0400
Subject: TEM-looking for a for-hire independent FIB company that does

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Try FIBICS in Ottawa, Canada. Contacts are Mike Phaneuf or Louise Weaver.
Their telephone number is 613-860-0861.
email: mphaneuf-at-fibics.com or lweaver-at-fibics.com

Jean-Pierre Slakmon
Soquelec Limited
5757 Cavendish Boulevard, Suite 101
Montreal, Quebec
Canada H4W 2W8
Tel: 514-482-6427 / Fax: 514-482-1929
URL: http://www.soquelec.com



-----Original Message-----
} From: Mike Coviello [mailto:coviello-at-mae.uta.edu]
Sent: Monday, June 19, 2000 4:12 PM
To: listserver


Hi Y'all:
We are looking for a for-hire independent FIB company that has
experience in preparing TEM cross-sections of semiconductors. Please
contact me if you do this, or know of a lab that does.
Regards,
Michael Coviello
Lab Manager
Materials Science
University of Texas at Arlington




From daemon Mon Jun 19 18:23:34 2000



From: Frank Macaluso :      macaluso-at-aecom.yu.edu
Date: Mon, 19 Jun 2000 18:00:27 -0400
Subject: Re: TEM of yeast

Contents Retrieved from Microscopy Listserver Archives
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Hi Lee,
We follow a very similar protocol to yours with the exception that we use
propylene oxide rather than ethanol for infiltration.
PO : Spurrs 1:1 1 hour
PO : Spurrs 1:3 1- 2 hours
Spurrs 1 - 2 hours
Spurrs overnight (we do not infiltrate under vacuum)
fresh Spurrs 1 - 2 hours
polymerization at 60C 48 hours

We had the problem you describe when we tried to embed yeast in EPON
equivalents. Switching to Spurrs was the fix for us.
Frank

At 09:46 AM 6/19/00 -0400, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Mon Jun 19 18:33:38 2000



From: Jon Mulholland :      jwm-at-genome.stanford.edu
Date: Mon, 19 Jun 2000 16:23:07 -0700 (PDT)
Subject: Re: TEM of yeast

Contents Retrieved from Microscopy Listserver Archives
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Hi Leona,
The standard way for dealing with yeast cells is to remove the
cell wall. This is done with glusulase (fancy name for snail guts) and or
lyticase (both from Sigma). If the wall cannot be removed for
experimental reasons (i.e. study of the plasma membrane/cell wall
interface) then you need to modify the carbohydrate linkages of the cell
wall to make the wall more permeable. This can be done by treating the
cells, after fixation, with 1% sodium metaperiodate for about 15 minutes.
However, most researchers simply wishing to examine yeast morphology
remove the wall because this not only improves inflitration of the resin
it also allows more extraction of the cytoplasm and thus makes it easier
to resolve structures and membranes within the ribosome rich yeast cell. A
classic protocol, by Byers and Goetsch, can be found in Vol 194, Methods
in Enzymology (AKA Guthrie and Fink) pg 602. I strongly encourage you to
read this article as yeast can be very problematic. You also want to use
the Hard Spurrs formulation and use 100% acetone (or propylene oxide) as
the last dehydration step before going into 1:1 resin. I have not
observed any difference in the ultrastructure of cells embedded in Spurrs
vs. polybed 812 (when the wall is removed).
If you want to do immuno-EM you might find our protocol useful;
checkout:
http://genome-www.stanford.edu/group/botlab/protocols/EM_protocol.pdf.

Jon Mulholland
Genetics Dept
Stanford University School of Medicine
Stanford, CA 94305-5120


On Mon, 19 Jun 2000, Leona Cohen-Gould wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi All,
} I processed a series of pellets of yeast for a client using a glut-pfa fix,
} osmium, dehydration through ethanols, and a day & a half step-wise
} infiltration into Spurr's resin (1:1 with Ethanol for 4 hr, pure resin
} overnight under light vacuum, then fresh for 3 more hours, then embed in
} fresh). Polymerization was overnight at 60C. About half the blocks were
} soft and had to be returned to the oven for a prolonged polym. (over the
} weekend). I was able to get sections from each of the 10 samples, but in
} the 'scope, many of these looked a bit like swiss cheese. those that were
} not lacy exhibited areas where the resin pulled away from the cell coats of
} the yeasts. Clearly something went wrong with the
} infiltration/polymerization. I used the same batch of resin for other
} things, and its fine.
}
} Does anyone out there have experience with yeast? Any suggestions?
} I'd like to give this peson some usable data!
}
} Thanks in advance,
} Lee
}
} Leona Cohen-Gould, M.S.
} Sr. Staff Associate
} Director, Electron Microscopy Core Facility
} Manager, Optical Microscopy Core Facility
} Joan & Sanford I. Weill Medical College
} of Cornell University
} voice (212)746-6146
} fax (212)746-8175
}
}
}



From daemon Tue Jun 20 07:07:44 2000



From: Keith Ryan :      kpr-at-ccms.ac.uk
Date: Tue, 20 Jun 2000 08:16:55 +0100
Subject: Re: Formvar & 1,2 dichloroethane

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Jonathan

Formvar in chloroform works well. I don't think we have ever tried
dochloroethane.

I have had to do the job myself recently and made a minor discovery -
the film seems to stick very well to acid washed slides! So, not so
cleverly clean. Then the second trick - float the film soon after it
has dried, within a minute. Then it seems to work better.

Keith

_______________________
Keith Ryan (Dr)
Marine Biological Association
Citadel Hill
Plymouth
Devon PL1 2PB
England

Tel. 0044 (0)1752 633279 (with answer machine)
also 0044 (0)1752 633249
Fax. 0044 (0)1752 633102

e-mail: kpr-at-wpo.nerc.ac.uk



From daemon Tue Jun 20 07:07:45 2000



From: Ian MacLaren :      maclariz-at-yahoo.co.uk
Date: Tue, 20 Jun 2000 08:40:52 +0100 (BST)
Subject: RE: Ceramic cross-section preparation

Contents Retrieved from Microscopy Listserver Archives
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Dear all,
Thanks to all who responded to my enquiry and gave me useful advice concerning
the preparation of cross-sections of ceramic thin films.

Hopefully, I should now be able to prepare some better thin film specimens
using one or more of the suggestions that I received.

Thanks again

Best wishes

=====
Ian MacLaren
Beijing Laboratory of Electron Microscopy
Chinese Academy of Sciences, P.O. Box 2724
100080 Beijing
China
General Email: ian.maclaren-at-physics.org
Work (esp. large attachments): maclaren-at-image.blem.ac.cn

____________________________________________________________
Do You Yahoo!?
Get your free -at-yahoo.co.uk address at http://mail.yahoo.co.uk
or your free -at-yahoo.ie address at http://mail.yahoo.ie



From daemon Tue Jun 20 07:07:46 2000



From: Krzysztof Jan Huebner :      hubner-at-IOd.krakow.pl
Date: Tue, 20 Jun 2000 11:34:53 +0200 (MET DST)
Subject: SCIA2001, First Announcement and Call for Papers: (fwd)

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12th Scandinavian Conference
on Image Analysis
SCIA 2001

June 11-14, 2001 in Bergen, Norway

Sponsored by: IAPR (The International Association
for Pattern Recognition)
http://www.iapr.org

First Announcement and Call for Papers:

http://www.ux.his.no/scia2001/

Invitation to the 12th SCIA.
Following the previous conferences in Greenland, SCIA 2001 -
the 12th Scandinavian Conference on Image Analysis - will be
held in Bergen on the west coast of Norway. The conference is
arranged by the Norwegian Society for Image Processing and
Pattern Recognition (NOBIM) and sponsored by the International
Association for Pattern Recognition (IAPR). The conference
venue is Grieghallen, located in the city centre.

Scientific Program:
The conference will offer internationally acclaimed speakers in
plenary talks and parallel sessions with selected oral presentations
and posters. The conference language is English.
The different presentations will cover unpublished theoretical or
applied research results.

Invited Speakers:
Professor Theo Pavilidis, State University of New York at Stony
Brook, USA: "History of Image Analysis"
Professor Josef Begün, University of Halmstad, Sweden:
"Biometric Person Authentication"
Professor Matti Pietikäinen, University of Oulu, Finland:
"Machine Vision and Media Processing"
Associate Professor Torbjørn Eltoft, University of Tromsø, Norway:
"Neural Network approaches to Cluster-Detection-and-Labelling"


In addition, we are working to find an invited speaker for the
subject: "Images in the future mobile terminals".

Pre-conference Workshop/Tutorial:
A set of pre-conference half-day workshops/tutorials will be held
on June 11, 2001:
1. ICA (Independent Component Analysis): Professor Erkki Oja,
Helsinki University of Technology,
Finland.
2. Data fusion: Professor Jon Atli Benediktsson,
University of Iceland.

Conference topics:
* Image analysis
* Computer vision
* Pattern recognition
* Neural networks
* Statistical methods
* Industrial applications
* Multimedia
* Biomedical applications
* Remote sensing
* Future technologies

Paper submission and registration for presenting authors:
Only full papers in English will be accepted, and the length should
not exceed eight pages. All papers will be refereed by two
reviewers for publication in the conference proceedings.
Please send four copies of your paper to:

SCIA2001,
Department of Electrical and Computer Engineering,
Stavanger University College,
P.O.Box 2557 Ullandhaug,
N-4091 Stavanger,
Norway

Important dates:

Paper submission deadline: November 6, 2000
Notification of acceptance: January 19, 2001
*Camera-ready copy: March 19, 2001

*Camera-ready copy must be accompanied by registration
and payment by presenting author.
The cover page must contain:
* Title of the paper
* Name(s), complete address and e-mail for the author(s)
* Brief abstract (150-200 words)
* Keywords describing the main subject of the paper (3-5 words)

* Author's opinion on whether the paper is most suitable for oral
or poster presentation
* Name and address for correspondence

Papers considerably longer than the final size, risk being rejected.
The fee for one or two extra pages is NOK 500 per page.
The fee for colour illustrations is NOK 4000 per page.
The decision on oral or poster presentation will be taken solely on
suitability, not on paper quality. Paper submission information is
available at http://www.ux.his.no/scia2001/, where the LaTeX style
file and an example file in the recommended two-column LaTeX
format is available.

Enquires:
If you have scientific questions, please contact
Ivar.Austvoll-at-tn.his.no. The program with further information about
registration and payment will be send to you February 1, 2001.
If you occasionally have seen this announcement, and want to have
the program sent to you, please contact scia-at-plus-convention.no.

Program Committee:
Dr. Ivar Austvoll (Chairman), Norway
Prof. Jussi Parkkinen, Finland
Prof. Fritz Albregtsen, Norway
Dr. Alfred Hanssen, Norway
Prof. Gunilla Borgefors, Sweden
Dr. Anne Solberg, Norway
Dr. Bjarne Ersbøll, Danmark

Organized by: Norsk forening for bildebehandling og
mønstergjenkjenning (NOBIM)
(Norwegian Society for Image Processing and
Pattern Recognition) http://www.nobim.no/

SCIA2001 web site:

http://www.his.no/scia2001/ or http://www.ux.his.no/scia2001/





From daemon Tue Jun 20 12:43:50 2000



From: Leona Cohen-Gould :      lcgould-at-mail.med.cornell.edu
Date: Tue, 20 Jun 2000 08:54:03 -0400
Subject: Re: Sperm Processing for TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 12:42 PM -0500 6/19/0, Connie A Cummings wrote:

} Good day to all on the listserver,
}
} I have a person in my department who is interested in processing horse
} sperm for TEM. Does anyone who does this routinely be willing to give me
} some pointers as to how to process them? I've only worked with muscle and
} brain tissue so this is kinda new - I have processed cells from cell
} culture for TEM would it be the same procedure?
}
} Thanks so much,
} Connie Cummings, DVM
} Instructor Anatomic Pathology
} Department VBP
} Oklahoma State University
****************************
Connie,
Assuming that your colleague will bring you a semen sample, and that
orientation is not critical, you can spin the sperm to a pellet and treat
it like any other cell pellet. I've done various rodent, marsupial and
human sperm samples this way. Once at the microscope, you will have to
hunt around a bit to fine the appropriate views (head, mid-piece,
tails,etc), but since there are so many cells in the pellet, I've always
found what we wre looking for. Its the easiest way to go.
Good luck,
Lee

Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175




From daemon Tue Jun 20 12:43:51 2000



From: Paula Allan-Wojtas :      AllanWojtasP-at-Em.agr.ca
Date: Tue, 20 Jun 2000 10:09:25 -0400
Subject: power problems with the SEM

Contents Retrieved from Microscopy Listserver Archives
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Hi, all,

I am posting this question to see if we can get some help for our building engineer to deal with problems we are having with our SEM (JEOL T330A).

We suspect that we are having a problem with the building power supply to our SEM. Other folks in our building using other types of equipment have found it necessary to use power conditioners or UPS systems to run their equipment - these systems seem to do the trick for them.

We are trying this approach with the power supply to our SEM, but find that there are some problems. When using the UPS system, we have to connect it up, put it on bypass, start up the SEM, then switch the UPS over after that - a real nuisance. If we don't do this, the SEM won't start.

Does anyone else have this problem, and if so, has found an easy solution? Please contact me offline if you have any suggestions and other words of wisdom. Thanks in advance. Also, thanks for all the help you as a group have given me when I posted previous questions - sometimes I forget to say thank you.

Paula.

Paula Allan-Wojtas
Research Scientist - Food Microstructure
Agriculture and Agri-Food Canada
Atlantic Food and Horticulture Research Centre
Kentville, Nova Scotia Canada B4N 1J5

Tel: (902) 679-5566
FAX: (902) 679-2311

email: allanwojtasp-at-em.agr.ca



From daemon Tue Jun 20 12:43:51 2000



From: John F. Mansfield :      jfmjfm-at-engin.umich.edu
Date: Tue, 20 Jun 2000 10:13:28 -0400
Subject: A Gatan User Meeting at M&M Philadelphia

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This is a preliminary message to all Gatan users who will be
attending the Microscopy and Microanalysis Meeting in Philadelphia
this August (13th to the 17th). I think it has become time to form a
User's Group to discuss the level of service and support that we are
receiving from Gatan. We should determine where the company should
be focusing its efforts and lobby them to correct problems that are
most important to us. Please let me know if you are interested in
attending such a meeting so I can gauge whether it should be held,
and how large a conference room I would need to reserve in Philly.

Note, Gatan representatives are encouraged to attend this meeting,
but it will be a user meeting run by the users.

I will not rant and rave here in a completely open forum, as I
believe it would be unfair. If you have concerns or comments on this
subject, whether or not you are attending M&M2000, please contact me
directly. Do NOT reply to the list, check the "To:" header before
sending your message, it should say "jfmjfm-at-engin.umich.edu" only.

Thank You.

John Mansfield.

Disclaimer: Opinions expressed in this message are my own personal
ones and do not represent necessarily those of my employers.

--

Dr. John Mansfield CPhys MInstP
North Campus Electron Microbeam Analysis Laboratory
417 SRB, University of Michigan
2455 Hayward, Ann Arbor MI 48109-2143
Phone: (734) 936-3352 FAX (734) 763-2282
Cellular Phone: (734) 358-7555
(Leaving a phone message at 936-3352 is preferable to 358-7555)
Email: jfmjfm-at-engin.umich.edu
URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html
Location: Lat. 42¡ 16' 48" Long. 83¡ 43' 48"



From daemon Tue Jun 20 12:43:54 2000



From: David H. Hall :      hall-at-aecom.yu.edu
Date: Tue, 20 Jun 2000 11:52:26 -0400
Subject: acetone + LR Gold

Contents Retrieved from Microscopy Listserver Archives
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Does anyone have experience taking specimens from acetone into LR Gold
resin? Some of the brochures on LR White seem to recommend against it, but
we are hoping it may be OK for LR Gold. We are doing freeze substitution
through acetone for EM-immunocytochemistry.

Thanks in advance for your help.
David H. Hall
Center for C. elegans Anatomy
Department of Neuroscience
1410 Pelham Parkway
Albert Einstein College of Medicine
Bronx, NY 10461

phone (718) 430-2195 FAX (718) 430-8821
hall-at-aecom.yu.edu
website: www.aecom.yu.edu/wormem



From daemon Tue Jun 20 12:53:57 2000



From: White, Woody N :      Woody.N.White-at-mcdermott.com
Date: Tue, 20 Jun 2000 13:17:00 -0500
Subject: Re:power problems with the SEM

Contents Retrieved from Microscopy Listserver Archives
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Paula,

Sounds like the UPS capacity (VA) is sufficient to run the system, but under
rated for the start-up surge. One solution would be a higher capacity UPS.
One
sized to handle the surge load, however, could be quite large and expensive.

If the nature of your problems is related to line noise, but not voltage
levels,
you might consider an "ultra isolation transformer" and experiment with
various
grounding options to minimize interference.

If voltage flucuations are the problem, investigate a "ferro resonant"
transformer to stabilize the line. This transformer *should* be somewhat
more
economical than a similar capacity UPS. BEWARE: These devices produce a
loud
hum. You don't want it in the same room without some sort of noise
attenuation.


.Haven't checked relative pricing, but here is a typical link:
http://www.sola-hevi-duty.com/

Another posibility... Can you seperate the vacuum pump(s) supply from the
electronics, powering the pumps directly and using your UPS for only the
electronics? That may lessen the start-up load enough for the UPS to
funcion
normally.

Woody White
McDermott Technology, Inc.

The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Hi, all,

I am posting this question to see if we can get some help for our building
engineer to deal with problems we are having with our SEM (JEOL T330A).

We suspect that we are having a problem with the building power supply to
our
SEM. Other folks in our building using other types of equipment have found
it
necessary to use power conditioners or UPS systems to run their equipment -
these systems seem to do the trick for them.

We are trying this approach with the power supply to our SEM, but find that
there are some problems. When using the UPS system, we have to connect it
up,
put it on bypass, start up the SEM, then switch the UPS over after that - a
real
nuisance. If we don't do this, the SEM won't start.

Does anyone else have this problem, and if so, has found an easy solution?
Please contact me offline if you have any suggestions and other words of
wisdom.
Thanks in advance. Also, thanks for all the help you as a group have given
me
when I posted previous questions - sometimes I forget to say thank you.

Paula.

Paula Allan-Wojtas
Research Scientist - Food Microstructure
Agriculture and Agri-Food Canada
Atlantic Food and Horticulture Research Centre
Kentville, Nova Scotia Canada B4N 1J5

Tel: (902) 679-5566
FAX: (902) 679-2311

email: allanwojtasp-at-em.agr.ca



From daemon Tue Jun 20 16:07:22 2000



From: tellis2-at-hallmark.com
Date: Tue, 20 Jun 2000 13:56:33 -0500
Subject: EDS Window

Contents Retrieved from Microscopy Listserver Archives
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HI:
For the second year in a row I am having to have our thin window (low
element type-brand name with held) replaced.
We have the EDS connected to a TOPCON SM-500 environmental SEM. I look at a
lot industrial dusts for particle size and composition and the manuf. of
the EDS system says they poke holes in the polymer window.
Has anyone else have this problem?
I have elected to have a thin beryllium installed this time since my
boss is upset about spending $7000 every year plus the downtime and since
low element detection is not critical .
Thanks
Terry Ellis
Hallmark Cards Inc.



From daemon Tue Jun 20 16:07:23 2000



From: carla_aiwohi-at-usgs.gov
Date: Tue, 20 Jun 2000 13:37:19 -0700
Subject: TEM cell pellets in agarose

Contents Retrieved from Microscopy Listserver Archives
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Hi all,

Does anyone have suggestions for encapsulating cell pellets in agarose?

I am working with flatfish cells but the pellets don't look cohesive enough
to withstand washing, dehydration etc.
We have some Type I agarose (Sigma, gel temp 36C, melting temp. 86C). I
plan to post-fix in 1% osmium tetroxide followed by 1% aqueous uranyl
acetate then embed in Spurr's resin.

As this is my first time working with cells, any help will be greatly
appreciated.

Thank you very much,
Carla Aiwohi
Western Fisheries Research Center
Seattle, WA



From daemon Tue Jun 20 16:07:24 2000



From: White, Woody N :      Woody.N.White-at-mcdermott.com
Date: Tue, 20 Jun 2000 16:57:00 -0500
Subject: Re:EDS Window

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Terry,

The particulate (have also heard) can "shoot" holes in a thin window. My
former
SEM/EDS was an Etec with a LARGE roughing system and a turret detector with
Be,
UTW, and "open" positions. Both windows lasted the 15 odd years it was in
use
before replacement. An important point is to evacuate and vent slowly so as
to
not accelerate the particles into the window.

I added a manual valve to the Etec between the roughing system and the
chamber.
With the automatic valves closed, I could slowly open the manual valve to
rough
the chamber down to a point where any particulate is not disturbed, close
the
valve, then switch to automatic to finish the evacuation. Venting gas was
from
my cryo of liquid nitrogen (pretty dry!) which I pressure/flow controlled
for
similar results on venting.

My modification was driven by the need to avoid damage to fluffy ceramic
specimens, but worked well to protect the detector also.

Have not added such a feature to the new SEM/Thin Window EDS system, but
(for
now) the chamber is pristine and the fluffy specimens are not part of the
work
mix.

Woody White
McDermott Technology, Inc

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


HI:
For the second year in a row I am having to have our thin window (low
element type-brand name with held) replaced.
We have the EDS connected to a TOPCON SM-500 environmental SEM. I look at a
lot industrial dusts for particle size and composition and the manuf. of
the EDS system says they poke holes in the polymer window.
Has anyone else have this problem?
I have elected to have a thin beryllium installed this time since my
boss is upset about spending $7000 every year plus the downtime and since
low element detection is not critical .
Thanks
Terry Ellis
Hallmark Cards Inc.



From daemon Tue Jun 20 17:58:18 2000



From: Earl Weltmer :      earlw-at-pacbell.net
Date: Tue, 20 Jun 2000 15:50:36 -0700
Subject: Re: power problems with the SEM

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Hi,

My guess is that the SEM inititally draws power that exceeds the ratings of the UPS.
It is probably due to the rotary pumps that can take up to ten amps upon start up.

The alternatives are to increase the power rating of the UPS or rewire the rotary pump so it draws it's power from the line and not through the UPS (the pumps is not that sensitive anyway).

Good Luck,

Earl weltmer

Paula Allan-Wojtas wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hi, all,
}
} I am posting this question to see if we can get some help for our building engineer to deal with problems we are having with our SEM (JEOL T330A).
}
} We suspect that we are having a problem with the building power supply to our SEM. Other folks in our building using other types of equipment have found it necessary to use power conditioners or UPS systems to run their equipment - these systems seem to do the trick for them.
}
} We are trying this approach with the power supply to our SEM, but find that there are some problems. When using the UPS system, we have to connect it up, put it on bypass, start up the SEM, then switch the UPS over after that - a real nuisance. If we don't do this, the SEM won't start.
}
} Does anyone else have this problem, and if so, has found an easy solution? Please contact me offline if you have any suggestions and other words of wisdom. Thanks in advance. Also, thanks for all the help you as a group have given me when I posted previous questions - sometimes I forget to say thank you.
}
} Paula.
}
} Paula Allan-Wojtas
} Research Scientist - Food Microstructure
} Agriculture and Agri-Food Canada
} Atlantic Food and Horticulture Research Centre
} Kentville, Nova Scotia Canada B4N 1J5
}
} Tel: (902) 679-5566
} FAX: (902) 679-2311
}
} email: allanwojtasp-at-em.agr.ca



From daemon Tue Jun 20 22:15:09 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Tue, 20 Jun 2000 21:22:29 -0500
Subject: Administrivia: Microscopy Listserver Search Engine Now On-Line

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Colleagues....

A search engine for the Microscopy Listserver Archives is now on-line.

You can access it throught the Listserver Home Page at:

http://www.msa.microscopy.com/MicroscopyListserver

You can search the entire ~ 7 year history of the Listserver
if your heart desires...

Search options allow you to search the Subject, Author, and Message Text
seperately or together.

Please let me know of any problems, since I wrote the search engine I'll
have to fix it when it breaks.

It's not perfect, but hey it works....

Cheers...

Nestor
Your Friendly Neighborhood SysOp




From daemon Tue Jun 20 22:15:11 2000



From: Walck, Scott D. :      walck-at-ppg.com
Date: Tue, 20 Jun 2000 22:53:54 -0400
Subject: Speaking of User group meetings - How about Emispec?

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John Mansfield's reminded me that I would like to have some sort of users'
group meeting for Emispec users for mutual support and information exchange.
I have discussed this with the Emispec folks, but I don't think that they
have followed up on it. Could I seed some level of support for a
get-together at M&M MM so that I can send it to Emispec. Please respond to
me offline and I will send them the number and names of the people that
would desire something like that. I would like to see something in a
positive and instructive type of meeting.


-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)




From daemon Wed Jun 21 08:34:26 2000



From: Mark Blackford :      mgb-at-ansto.gov.au
Date: Wed, 21 Jun 2000 15:32:31 +1100
Subject: Rotory pump oil

Contents Retrieved from Microscopy Listserver Archives
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Dear all,

this is a message for EM people in Sydney or Woolongong, Australia.

The rotory pump on my JEOL 2020F TEM is short of oil and making a bit of
noise so I'm trying to find some quickly. Our JEOL service guys have
ordered some "MR100" but it may take a couple of weeks to arrive. Can any
one lend me a few hundred mls in the meantime?

Can anyone suggest a local supplier of this rotory pump oil?

Cheers,


Mark Blackford
TEM Group
Materials Division, ANSTO
PMB 1,
Menai, N.S.W.
Australia
2234

Phone 61 2 9717 3027
Fax 61 2 9543 7179

Disclaimer:
The views expressed in this E-mail message do not necessarily represent the
official views of ANSTO from which this message was conveyed.




From daemon Wed Jun 21 08:34:27 2000



From: Larry Stoter :      LPS-at-teknesis.demon.co.uk
Date: Tue, 20 Jun 2000 17:49:44 +0100
Subject: Re: power problems with the SEM

Contents Retrieved from Microscopy Listserver Archives
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} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

My guess would be the current loading at start up. When you turn on
an SEM from cold, everything starts and draws current. In particular,
the rotary pump kicks in and draws a high current as it starts. You
will need to be able to set up the UPS so that it can handle this -
or set it on a short timer so that it automatically switches in, say,
10 mins after start up.

Regards,
--
Larry Stoter
JEOL (UK) Ltd
Silver Court, Watchmead, Welwyn Garden City, AL7 1LT, United Kingdom
tel: +44-(0)1707-377117, fax: +44-(0)1707-373254, e-mail: larrys-at-jeoleuro.com



From daemon Wed Jun 21 08:34:31 2000



From: Jo Verbeeck :      joverbee-at-ruca.ua.ac.be
Date: Wed, 21 Jun 2000 09:16:35 +0200 (METDST)
Subject: Re: power problems with the SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,

Probably you are having problems with the high switch on current the sem
draws. You could try switching on different parts of the SEM system not at
the same time (if possible). You could also consider to only power the
electronics of the SEM via the UPS, in that way you reduce the required
current drastically, while there is little use for a pump to sit on the
UPS power.
Alternatively you could look for a more powerfull UPS that can handle the
larger currents (for short moment is enough- see data sheets of different
UPS's).

Hope this helps...

Jo

*************************************************************
* Jo Verbeeck *
* University of Antwerp *
* Dept. EMAT (Electron Microscopy for Materials Research) *
* e-mail: joverbee-at-ruca.ua.ac.be *
* tel: +32(0)3 218 02 49 *
* fax: +32(0)3 218 02 57 *
*************************************************************

On Tue, 20 Jun 2000, Paula Allan-Wojtas wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi, all,
}
} I am posting this question to see if we can get some help for our building engineer to deal with problems we are having with our SEM (JEOL T330A).
}
} We suspect that we are having a problem with the building power supply to our SEM. Other folks in our building using other types of equipment have found it necessary to use power conditioners or UPS systems to run their equipment - these systems seem to do the trick for them.
}
} We are trying this approach with the power supply to our SEM, but find that there are some problems. When using the UPS system, we have to connect it up, put it on bypass, start up the SEM, then switch the UPS over after that - a real nuisance. If we don't do this, the SEM won't start.
}
} Does anyone else have this problem, and if so, has found an easy solution? Please contact me offline if you have any suggestions and other words of wisdom. Thanks in advance. Also, thanks for all the help you as a group have given me when I posted previous questions - sometimes I forget to say thank you.
}
} Paula.
}
} Paula Allan-Wojtas
} Research Scientist - Food Microstructure
} Agriculture and Agri-Food Canada
} Atlantic Food and Horticulture Research Centre
} Kentville, Nova Scotia Canada B4N 1J5
}
} Tel: (902) 679-5566
} FAX: (902) 679-2311
}
} email: allanwojtasp-at-em.agr.ca
}
}
}



From daemon Wed Jun 21 08:34:32 2000



From: Ron Doole :      ron.doole-at-materials.oxford.ac.uk
Date: Wed, 21 Jun 2000 08:36:37 +0100 (BST)
Subject: Re: EDS Window

Contents Retrieved from Microscopy Listserver Archives
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Hi Terry,

If you are using water vapour in your environmental SEM you may
want to check the suitability of a Be window. I have a vague memory that
water will make holes in the thin (6-8um) Be, check it out with your
supplier.

I have used two polymer windows on an EDX detector in a TEM
gas reaction cell. The front window is on a replaceable mount in front of
the detector. This is easily exchangeable in case I cover it with reaction
products from the in-situ experiment; I don't want to see them in all
subsequent spectra. Much cheaper then a window replacement and can be
carried out by me on site. Contact me for further details if you want to.

Reducing the disturbance to the vacuum system gasses during
pumpdown and venting by limiting the speed may help prevent dust particles
damaging the window in the first place.

Regards,
Ron

On Tue, 20 Jun 2000 tellis2-at-hallmark.com-at-sparc5.microscopy.com wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} HI:
} For the second year in a row I am having to have our thin window (low
} element type-brand name with held) replaced.
} We have the EDS connected to a TOPCON SM-500 environmental SEM. I look at a
} lot industrial dusts for particle size and composition and the manuf. of
} the EDS system says they poke holes in the polymer window.
} Has anyone else have this problem?
} I have elected to have a thin beryllium installed this time since my
} boss is upset about spending $7000 every year plus the downtime and since
} low element detection is not critical .
} Thanks
} Terry Ellis
} Hallmark Cards Inc.
}
}
}

===========================================================================
Mr. Ron Doole e-mail ron.doole-at-materials.ox.ac.uk
Department of Materials, phone +44 (0) 1865 273701
University of Oxford, fax +44 (0) 1865 283333
Parks Road.
Oxford. OX1 3PH. UK.
============================================================================



From daemon Wed Jun 21 08:34:39 2000



From: Your Name :      KMG1TEX-at-bolton.ac.uk
Date: Wed, 21 Jun 2000 12:36:19 +0100
Subject: student requires information

Contents Retrieved from Microscopy Listserver Archives
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Dear Sir/Madam

I am currently studying for an MSc in Textile studies, at Bolton
Institute, England.

For part of my studies I have been analysing some polyester film that
has been treated with 10% sodium hydroxide under imposed load under
polarized light on an optical microscope. Vivid colours have been noted,
Reds, greens, and I am struggling to find information to outline what
these colours are actually indicating. I therefore write and ask for any
information you may deem relevant, I would be extremely grateful for.

I thank you for your time.

Yours Faithfully

Kelly Goodman

EMAIL: suite666-at-netscapeonline.co.uk



From daemon Wed Jun 21 08:34:40 2000



From: Leroux christine :      leroux-at-univ-tln.fr
Date: Wed, 21 Jun 2000 14:07:10 +0200
Subject: EDS analysis of Co in WC-Co

Contents Retrieved from Microscopy Listserver Archives
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Hi,
I am working on powders of mixed WC and Co and I have some problems with EDS.
EDS analysis on grains whose diffractions patterns are unambiguously
indexed in the Co structure give a majority of W, and EDS analysis on
grains whose diffractions patterns are unambiguously indexed in the WC
structure (without distortion and superstructures) always indicate the
presence of Co (with a majority of W). So I was wondering if there could be
any problem with EDS on Cobalt (because of magnetism????)

christine

**************************************
Christine leroux
Lab. M.M.I
Universite de Toulon-Var, Bat.R
B.P.132
F-83957 La Garde Cedex
tel: 00 33 (0) 4 94 14 25 07
fax: 00 33 (0) 4 94 14 21 68

**************************************



From daemon Wed Jun 21 08:44:49 2000



From: Robert.Carlton-at-aventis.com
Date: Wed, 21 Jun 2000 08:35:34 -0500
Subject: Re: EDS Window

Contents Retrieved from Microscopy Listserver Archives
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Terry,

I have an Electroscan 2010 and routinely have problems with the window. I
have ascribed it to particles flying around from the surface of the sample.
This generally occurs at startup when there may be some charging. We solved
the problem by retrofiting the detector so that it can be retracted from the
chamber when changing specimens. Its a pain in the butt, but less so than
having to replace windows.

Thanks
Robert Carlton
Aventis Pharmaceuticals
robert.carlton-at-aventis.com




From daemon Wed Jun 21 17:28:58 2000



From: Tobias Baskin :      BaskinT-at-missouri.edu
Date: Wed, 21 Jun 2000 09:06:04 -0500
Subject: Re: student ... (LM:polarized light)

Contents Retrieved from Microscopy Listserver Archives
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Greetings,
Yes, those colors are beautiful, arn't they?
The colors indicate the magnitude of birefringent retardation
in your sample. In essence, you are seeing subtraction colors
resulting from the diminution of intensity at certain wavelengths.
Say your sample has a retardation of 550 nm. When linearly polarized
light of that wavelength passes through the sample, it will be
retarded by exactly one wavelength, which is the same as zero
retardation. Thus, it will not be affected by the sample and will be
blocked by the analyzer. But light of longer or shorter wavelengths
will be retarded by more or less than a wave and so will emerge as
elliptically polarized light and thus will have a component
transmitted through the analyzer.
So for any actual sample retardation, the color will result
from exactly how much of each wavelength gets through. Because our
eyes are very sensitive to color, this has been used for more than
100 years to measure/estimate retardation. There is a chart that
reproduces the colors as a function of retardation. I have no idea if
this chart has made it on line but I would be careful. The colors are
very tricky to print and great care was used to get them right. You
would do far better to look this one up in your library. I am sure
the Bolton Textile Institute will have excellent books on polarized
light microscopy, and while they might be dusty, this particular
corner of science has been well understood for years and years.
Hope this helps,
Tobias Baskin



}
}
} Dear Sir/Madam
}
} I am currently studying for an MSc in Textile studies, at Bolton
} Institute, England.
}
} For part of my studies I have been analysing some polyester film that
} has been treated with 10% sodium hydroxide under imposed load under
} polarized light on an optical microscope. Vivid colours have been noted,
} Reds, greens, and I am struggling to find information to outline what
} these colours are actually indicating. I therefore write and ask for any
} information you may deem relevant, I would be extremely grateful for.
}
} I thank you for your time.
}
} Yours Faithfully
}
} Kelly Goodman
}
} EMAIL: suite666-at-netscapeonline.co.uk

_ ____ ^ __ ____ Tobias I. Baskin
/ \ / / \ / \ \ University of Missouri
/ | / / \ \ \ Biological Sciences
/___/ /__ /___ \ \ \__ 109 Tucker Hall
/ / / \ \ \ Columbia, MO 65211-7400 USA
/ / / \ \ \ voice: 573-882-0173
/ /____ / \ \__/ \____ fax: 573-882-0123



From daemon Wed Jun 21 17:29:02 2000



From: Sue Danielson :      sdaniels-at-post.its.mcw.edu
Date: Wed, 21 Jun 2000 10:11:03 -0500
Subject: TEM-micrographs for teaching manual

Contents Retrieved from Microscopy Listserver Archives
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Hello everyone,

A colleage of mine here at the Medical College of Wisconsin is working on a
new teaching manual for our first-year medical students' cells & tissues
course. We are currently putting together a portion of the manual which
shows students electron micrographs of cells and organelles. We are looking
for high quality TEM images (non-copyrighted) of structures such as the
following:

Cytoskeleton (actin microfilaments, microvilli, microtubules, centrioles)

Cell Membrane (particularly intercellular junctions such as z. occludens,
adherens, desmosomes)

Protein synthesis & vescicles ( ribosomes, RER, golgi, endosomes,
lysosomes, peroxisomes, & vescicles-coated, endocytotic, secretory)

Nucleus (nuclear envelope, nuclear pores, chromatin, nucleolus)

MItochondria

We would be grateful to anyone who feels they have something useful to
contribute. The plan is to scan appropriate images for the lab manual and
return them as soon as possible to the owner. There will be a list at the
end of the manual acknowledging all contributors.

Thank you everyone,

Susan K. Danielson, MS
Neuromuscular Lab Coordinator
Dept. Neurology, Medical College of Wisconsin
ph: 414.259.3836
email: sdaniels-at-mcw.edu








From daemon Wed Jun 21 17:29:07 2000



From: Stephen Page :      stevep-at-micrometsys.com
Date: Wed, 21 Jun 2000 13:17:43 -0500
Subject: Job Posting-Technical Representative

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Here we grow again! We need more help.

We are looking for qualified professionals to provide applications-based
sales and technical support for our complete line of optical microscopes,
sample preparation equipment, consumables, and digital imaging systems in
the Dallas/Ft. Worth and Austin, TX areas. Must have at least 2 years
experience in failure analysis or materials analysis using microscopes and
sample preparation equipment. Prior sales experience is not necessary.
Understanding of dimensional measurement and image analysis systems a plus.

Please respond by e-mail to mms-at-micrometsys.com



From daemon Wed Jun 21 17:29:07 2000



From: mms :      mms-at-micrometsys.com
Date: Wed, 21 Jun 2000 13:21:38 -0500
Subject: Job Posting-Technical Representative

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Here we grow again! We need more help.

We are looking for qualified professionals to provide applications-based
sales and technical support for our complete line of optical microscopes,
sample preparation equipment, consumables, and digital imaging systems in
the Dallas/Ft. Worth and Austin, TX areas. Must have at least 2 years
experience in failure analysis or materials analysis using microscopes and
sample preparation equipment. Prior sales experience is not necessary.
Understanding of dimensional measurement and image analysis systems a plus.

Please respond by e-mail to mms-at-micrometsys.com



From daemon Wed Jun 21 17:29:08 2000



From: Gib Ahlstrand :      giba-at-puccini.cdl.umn.edu
Date: Wed, 21 Jun 2000 13:42:12 -0500 (CDT)
Subject: Re: acetone + LR Gold

Contents Retrieved from Microscopy Listserver Archives
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Responding to the message of
{3.0.5.32.20000620115226.008c7a80-at-mailserver.aecom.yu.edu}
from "David H. Hall" {hall-at-aecom.yu.edu} :

David,

I've done it, but the thing to watch out for is that if the temperature of your
acetone/LR Gold mixtures - say 1:1 acetone:LR Gold - drops below about -35 C,
some components of the LR Gold will start to freeze out, solutions gets cloudy.
For methanol sub-solution's, LR Gold starts to freeze out at even higher temps,
about -27 C.

Just experiment with your sub mixtures at various temperatures to see if you get
any freeze-out like this happening, before you do an actual sub run. If so just
make sure you stay warmer than freezing points.

I'm curious why this happens, anyone else got any info on this?

Gib Ahlstrand



} Does anyone have experience taking specimens from acetone into LR Gold
} resin? Some of the brochures on LR White seem to recommend against it, but
} we are hoping it may be OK for LR Gold. We are doing freeze substitution
} through acetone for EM-immunocytochemistry.
}
} Thanks in advance for your help.
} David H. Hall
} Center for C. elegans Anatomy
} Department of Neuroscience
} 1410 Pelham Parkway
} Albert Einstein College of Medicine
} Bronx, NY 10461
}
} phone (718) 430-2195 FAX (718) 430-8821
} hall-at-aecom.yu.edu
} website: www.aecom.yu.edu/wormem


Gib Ahlstrand
Electron Optical Facility, University of Minnesota, Dept. Plant Pathology
495 Borlaug Hall, St. Paul, MN. USA. 55108 (612)625-8249
612-625-9728 FAX, giba-at-puccini.cdl.umn.edu
http://biosci.umn.edu/MIC/consortium.html



From daemon Wed Jun 21 17:29:11 2000



From: Bob Price :      price-at-dcsmserver.med.sc.edu
Date: Wed, 21 Jun 2000 15:38:04 EDT
Subject: suggestions for symposia for M&M 2001 meeting

Contents Retrieved from Microscopy Listserver Archives
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Hi,

Even though we have not yet had our Microscopy and Microanalysis 2000
meeting in Philadelphia the Program Committee for the M&M 2001
meeting is preparing the symposia topics for the meeting in Long
Beach. If anyone has suggestions for topics that they would like to
see included in the Long Beach meeting please contact one of the
Program Committee Officers listed below. Please note that we may not
be able to respond to all suggestions for this year, but voicing your
opinion now may get a topic of interest on the program list for
upcoming years.

Thanks.

Bob Price

Program Officers:

Bob Price, M&M 2001 Program Chair
Price-at-med.sc.edu

Inga Holl Musselman, M&M 2001 MAS Program Co-chair
imusselm-at-utdallas.edu

Edgar Voelkl, M&M 2001 Program Vice-chair/M&M 2002 Program Chair
vog-at-ornl.gov

Robert L. Price
Director, Instrumentation
Resource Facility
USC School of Medicine
Garner's Ferry Road
Columbia, SC 29208
Phone: 803-733-3393
Fax:803-733-1533



From daemon Wed Jun 21 17:29:15 2000



From: jjerome-at-wfubmc.edu (Jay Jerome)
Date: Wed, 21 Jun 2000 17:07:19 -0400
Subject: Osmium disposal

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I couldn't find this in the archives but I cannot imagine someone has
not asked this already. If so, please direct me to the approximate
month, year. If not, here goes:

We are decomissioning a laboratory refrigerator. Over the 30+ years of
use it has accumulated osmium black on the walls. It has been determined
by our EH&S people (after consultation with a hazardous waste shipping
compnay) that disposal requires a sealed, separate container for the
refrigerator, several licenses, and a hazardous waste truck. The
estimate is not in yet, but everyone is talking thousands of dollars. If
that is the only alternative for safe disposal, so be it. However, I
cannot imagine that there is not a safe alternative to decontaminate
before shipping. After all we clean up spills with corn oil and reduce
osmium tetroxide with 5% sodium bisulfite solution or sodium
metabisulfite. Any definitive advice or references will be
appreciated.

Thanks-

--
Jay
----------------------------------------------
- AKA: W. Gray Jerome, Ph.D. -
- Department of Pathology -
- Wake Forest University School of Medicine -
- Winston-Salem, NC 27157-1092 -
- Ph: 336-716-4972, 336-716-2675 -
- Fax: 336-716-6174 -
- E-mail: jjerome-at-wfubmc.edu -
----------------------------------------------




From daemon Wed Jun 21 19:04:52 2000



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Wed, 21 Jun 2000 19:56:33 -0500
Subject: Refrigerator osmium

Contents Retrieved from Microscopy Listserver Archives
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-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

W. Gray Jerome wrote:
===========================================================
I couldn't find this in the archives but I cannot imagine someone has not
asked this already. If so, please direct me to the approximate month, year.
If not, here goes:

We are decomissioning a laboratory refrigerator. Over the 30+ years of use
it has accumulated osmium black on the walls. It has been determined by our
EH&S people (after consultation with a hazardous waste shipping compnay)
that disposal requires a sealed, separate container for the refrigerator,
several licenses, and a hazardous waste truck. The estimate is not in yet,
but everyone is talking thousands of dollars. If that is the only
alternative for safe disposal, so be it. However, I cannot imagine that
there is not a safe alternative to decontaminate before shipping. After all
we clean up spills with corn oil and reduce osmium tetroxide with 5% sodium
bisulfite solution or sodium metabisulfite. Any definitive advice or
references will be appreciated.
=============================================================
I will risk showing what maybe I don't know, but this is really an important
question. Laboratories should not be wasting thousands of dollars
needlessly, when other alternatives are available.

I thought that Os (IV) oxide, that is, the tetroxide form of osmium, was
clear, after all, crystals in ampoules are fairly clear or translucent, and
4% aqueous osmium tetroxide is water clear. And I thought that in the
reduced form, that is, the dioxide or Os (II) form, the color was black.
Putting it another way, if it is in the reduced form (e.g. black), it is not
in its "hazardous" form, and indeed might even be fairly innocuous. A
**quick** survey of shipping regulations does not even indicate that osmium
(II) oxide is a controlled material from the standpoint of shipping. I want
to emphasize the word "quick", but I could not find a UN number asigned for
it.

If that is the case, and if I am right, why could not the black deposit be
collected, perhaps by way of some rubbing and scrubbing, and disposed of
(or better yet, recycled) and the refrigerator disposed of as any ordinary
refrigerator might be dealt with?

Remember, I am not saying that this could be done, legally or otherwise, I
am just asking what it is that I might be missing here.

Disclaimer: SPI Supplies is a major supplier of osmium tetroxide to EM
laboratories worldwide.

Chuck
============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.spi.cc
########################
============================================




From daemon Wed Jun 21 19:22:08 2000



From: Paul-Laurent Capron Jr. :      Paul-Laurent.capron.jr-at-laborimpex.be
Date: Wed, 21 Jun 2000 19:07:12 -0500
Subject: chemical reference standards for the analysis of a Ni-base alloy

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CAN SOMEBODY HELP ME?:

-We need chemical reference standards for the analysis of a Ni-base alloy.

-We intend to use the energy-dispersive X-ray analysis, coupled to a
Scanning
Electron Microscope(SEM).

-The composition of the alloy is going to vary between 18 to 28 wt%
Aluminium ;
Al is the most important element to be tested, the remainder of the alloy
being
mostly Ni of course.

-Secondary main elements are : Cr from 4 to 10 wt% ; Co from 10 to 15
wt% ; Elements in low concentration (below 3%) are Ti and Mo.

-Accuracy : 0.5 % in Al is required.


Questions are :

-How many different standards would we need ?

-What should be the chemical variations from standard to standard to reach
the
required accuracy ?

-What would be the price for such standards ? Time for delivery ? Where to
order this ?

-Can you send us information (reprints from technical journals, articles) on
the
topic of chemical analysis by means of energy-dispersive (or
wavelength-dispersive) X-ray systems ?

If you need any further information, please contact me.

In anticipation, many thanks for a prompt answer.

Yours faithfully,

PAUL-LAURENT CAPRON JR.
S.A. LABORIMPEX N.V.
RUE DES ALLIES 78-80
BONDGENOTENSTRAAT 78-80
1190 BRUSSEL/BRUXELLES
BELGIUM
TEL.: 00 32 2 345.99.94
FAX.: 00 32 2 347.39.63




From daemon Wed Jun 21 19:40:12 2000



From: Barry Searle :      B.Searle-at-unsw.edu.au
Date: Wed, 21 Jun 2000 19:23:37 -0500
Subject: JEOL Coater

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Fellow EM Users, Just a short query ----- would anyone installed a
modern day alternative to the 1920's !!! style glass ionization vacuum
gauge ("electron current") + electronics box for the JEOL 4B coater?
Thankyou for your time, Barry EM UNIT UNSW




From daemon Thu Jun 22 07:23:12 2000



From: jim :      jim-at-proscitech.com.au
Date: Thu, 22 Jun 2000 11:49:58 +1000
Subject: RE: Osmium disposal

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Very funny, in every meaning of that word. Of course, the waste company would
make a meal of it, why ask?

Its not Os04 that is coating the inside of the fridge, but a tiny amount of
osmium metal. The metal is not particularly toxic. Os and Ir are the heaviest
metals, with a specific gravity over 22, but nonetheless, its hard to see that
you would have 2g of Os in any refrigerator coating. Finely dispersed Os
produces tiny amounts of Os04 - if that is a problem, than that would have been
so in the confined lab space. If that fridge was dumped conventionally in a
land-fill, the tiny amount of OsO4 would be reduced before ever reaching the
surface. If it was smelted for scrap, arguably it would, however, immeasurably
improve the quality of the scrap.
There is an argument for smearing a bit of vegetable oil over the inside of the
fridge prior to disposal.
Have those safety people given their fully developed reasons? I love to know
them.

The bigger picture is interesting too. Safety people are not selected for, nor
seem to get taught much logic or a sense of proportion. So we seem to get more
and more hassles about things that hardly matter at all, but there is no money
and often no objection to the really large environmental issues. Its been said
that the old Roman civilasation's collapse was partially due to the difficult
Roman numerals, which made the efficient administration of the empire
impossible. Our "Western Society" has seen a rapid increase of useless, if not
counterproductive rules and regulations, I fear if that trend continues that
these "well-meaning" rules will undermine our society.
Cheers
Jim Darley

ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Thursday, June 22, 2000 7:07 AM, Jay Jerome [SMTP:jjerome-at-wfubmc.edu] wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I couldn't find this in the archives but I cannot imagine someone has
} not asked this already. If so, please direct me to the approximate
} month, year. If not, here goes:
}
} We are decomissioning a laboratory refrigerator. Over the 30+ years of
} use it has accumulated osmium black on the walls. It has been determined
} by our EH&S people (after consultation with a hazardous waste shipping
} compnay) that disposal requires a sealed, separate container for the
} refrigerator, several licenses, and a hazardous waste truck. The
} estimate is not in yet, but everyone is talking thousands of dollars. If
} that is the only alternative for safe disposal, so be it. However, I
} cannot imagine that there is not a safe alternative to decontaminate
} before shipping. After all we clean up spills with corn oil and reduce
} osmium tetroxide with 5% sodium bisulfite solution or sodium
} metabisulfite. Any definitive advice or references will be
} appreciated.
}
} Thanks-
}
} --
} Jay
} ----------------------------------------------
} - AKA: W. Gray Jerome, Ph.D. -
} - Department of Pathology -
} - Wake Forest University School of Medicine -
} - Winston-Salem, NC 27157-1092 -
} - Ph: 336-716-4972, 336-716-2675 -
} - Fax: 336-716-6174 -
} - E-mail: jjerome-at-wfubmc.edu -
} ----------------------------------------------
}
}



From daemon Thu Jun 22 07:23:12 2000



From: jim :      jim-at-proscitech.com.au
Date: Thu, 22 Jun 2000 12:01:59 +1000
Subject: RE: Rotory pump oil

Contents Retrieved from Microscopy Listserver Archives
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Rotary pump oil is a refined mineral oil, which needs to have low vapour
pressure, low viscosity and good lubricating properties. such oils are made by
"oil companies". In my lab days I never had the luxury to purchase oil from a
microscope manufacturer, because their on-cost for such items are horrific and
real and my budgets were small.

Disclaimer: ProSciTech sell Rotary Pump Oil. Don't even ask to ship it to USA;
the material is cheap, but expensive to ship. Our price includes shipping
within Australia only.
Cheers
Jim Darley

ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Wednesday, June 21, 2000 2:33 PM, Mark Blackford [SMTP:mgb-at-ansto.gov.au]
wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear all,
}
} this is a message for EM people in Sydney or Woolongong, Australia.
}
} The rotory pump on my JEOL 2020F TEM is short of oil and making a bit of
} noise so I'm trying to find some quickly. Our JEOL service guys have
} ordered some "MR100" but it may take a couple of weeks to arrive. Can any
} one lend me a few hundred mls in the meantime?
}
} Can anyone suggest a local supplier of this rotory pump oil?
}
} Cheers,
}
}
} Mark Blackford
} TEM Group
} Materials Division, ANSTO
} PMB 1,
} Menai, N.S.W.
} Australia
} 2234
}
} Phone 61 2 9717 3027
} Fax 61 2 9543 7179
}
} Disclaimer:
} The views expressed in this E-mail message do not necessarily represent the
} official views of ANSTO from which this message was conveyed.
}
}



From daemon Thu Jun 22 07:23:14 2000



From: jim :      jim-at-proscitech.com.au
Date: Thu, 22 Jun 2000 15:20:45 +1000
Subject: RE: JEOL Coater

Contents Retrieved from Microscopy Listserver Archives
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I remember using the old Geissler tube on an evaporator 32 years ago and very
likely they existed long before then. They were actually a bit of an ongoing
maintenance expense, but I loved the pretty colours. Cannot see any reasons why
other type gauges could not be used, except the manufacturer would find the
Geissler tube a cheaper alternative.

Disclaimer: ProSciTech supplies vacuum gauges, circa year 2000

Cheers
Jim Darley

ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Thursday, June 22, 2000 10:24 AM, Barry Searle [SMTP:B.Searle-at-unsw.edu.au]
wrote:
}
} Fellow EM Users, Just a short query ----- would anyone installed a
} modern day alternative to the 1920's !!! style glass ionization vacuum
} gauge ("electron current") + electronics box for the JEOL 4B coater?
} Thankyou for your time, Barry EM UNIT UNSW
}



From daemon Thu Jun 22 07:23:21 2000



From: Robert H. Olley :      r.h.olley-at-reading.ac.uk
Date: Thu, 22 Jun 2000 09:20:35 +0100 (BST)
Subject: Re: student requires information

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to: Kelly Goodman,

Greetings!

Regarding the colours in the PET film, the optical part is as Tobias
Baskin says. In regard to the polymer science side of it, sretched films
or fibres show optical anisotropy, because the polarizability along the
polymer chain is different from that transverse to it. The more highly
stretched the fibre, the more the chains will be oriented, and this will
increase the optical anisotropy of the fibre. Multiply this by the
thickness of the fibre and that will give you the birefringence. Most
likely you will be seeing coloured fringes parallel to the fibre
direction, which indicate the different viewing thickness through the
different parts of the fibre, just like the colours in an oil slick under
a car indicate different thicknesses of an oil film.

+------------------------------------------------------------------------+
| Robert H.Olley Phone: |
| J.J.Thomson Physical Laboratory {direct line +44 (0) 118 9318572 |
| University of Reading {University internal extension 7867 |
| Whiteknights Fax +44 (0) 118 9750203 |
| Reading RG6 6AF Email: R.H.Olley-at-reading.ac.uk |
| England URL: http://www.reading.ac.uk/~spsolley |
+------------------------------------------------------------------------+








From daemon Thu Jun 22 07:23:24 2000



From: Koh YinHsian-CYK006 :      Yinhsian.Koh-at-Motorola.com
Date: Thu, 22 Jun 2000 18:37:53 +0800
Subject: Interface Analysis

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Hello,

I am not familiar with microscopy. The most I know is how SEM/EDX can
examine a cross sectional sample.

Currently, I am looking for some methodology to analyse interface structure,
elementry and chemical compound especially between the solder and metal,
i.e. the IMC layer. The solder height will be ranging from 2-4 mil (SMT
process). Wondering anybody can advice the best method to analyse the IMC
layer. EDX is capable of detecting the element contain insde the IMC but I
am seeing more and more alloy up to ternary or quartenary alloy at the
layer. Problem is I do not have any idea about where and how it come from.

Appreciate your help.

Thank you.

Regards,
YH Koh



From daemon Thu Jun 22 07:23:25 2000



From: Kristian Ukkonen :      kukkonen-at-cc.hut.fi
Date: Thu, 22 Jun 2000 13:54:52 +0300 (EET DST)
Subject: JVG-N2 vacuum gauge and forgel tubes

Contents Retrieved from Microscopy Listserver Archives
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Hello,

I'm looking for a manual for the JVG-N2 ionization
vacuum gauge. I've already tried JEOL and they could
find JVG-N1 manual (thanks!), but not -N2, and -N1 and
-N2 are different enough (different connectors, apparently
EB grid heating in -N2 etc.) that the -N1 manual is not
enough to get the gauge operational.

So, anyone with manual for JVG-N2 ??

btw: The JVG-N1 manual mentions "Forgel tube" which looks
in drawing just like normal hot-filament ionization
gauge tube.. Is "Forgel tube" just an old term for one?

Thanks,

Kristian Ukkonen.
kukkonen-at-cc.hut.fi



From daemon Thu Jun 22 08:18:01 2000



From: White, Woody N :      Woody.N.White-at-mcdermott.com
Date: Thu, 22 Jun 2000 08:50:00 -0500
Subject: Re:Interface Analysis

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For some idea of what can be done, check out
the soldering iron tip images and x-ray
data at my web site.

Keep in mind that I did not not take time
to achieve the best possible polish and
most of the work was at rather low magnification.

Site: http://woody.white.home.att.net

Woody White

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Hello,

I am not familiar with microscopy. The most I know is how SEM/EDX can
examine a cross sectional sample.

Currently, I am looking for some methodology to analyse interface structure,
elementry and chemical compound especially between the solder and metal,
i.e. the IMC layer. The solder height will be ranging from 2-4 mil (SMT
process). Wondering anybody can advice the best method to analyse the IMC
layer. EDX is capable of detecting the element contain insde the IMC but I
am seeing more and more alloy up to ternary or quartenary alloy at the
layer. Problem is I do not have any idea about where and how it come from.

Appreciate your help.

Thank you.

Regards,
YH Koh



From daemon Thu Jun 22 08:18:01 2000



From: Donald Lovett :      lovett-at-tcnj.edu
Date: Thu, 22 Jun 2000 09:06:47 -0400 (EDT)
Subject: Re: Refrigerator osmium

Contents Retrieved from Microscopy Listserver Archives
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On Wed, 21 Jun 2000, Garber, Charles A. wrote:

} question. Laboratories should not be wasting thousands of dollars
} needlessly, when other alternatives are available.
}
} I thought that Os (IV) oxide, that is, the tetroxide form of osmium, was
} clear, after all, crystals in ampoules are fairly clear or translucent, and
} 4% aqueous osmium tetroxide is water clear. And I thought that in the
} reduced form, that is, the dioxide or Os (II) form, the color was black.
} Putting it another way, if it is in the reduced form (e.g. black), it is not
} in its "hazardous" form, and indeed might even be fairly innocuous. A
} **quick** survey of shipping regulations does not even indicate that osmium
} (II) oxide is a controlled material from the standpoint of shipping. I want
} to emphasize the word "quick", but I could not find a UN number asigned for
} it.
}
} If that is the case, and if I am right, why could not the black deposit be
} collected, perhaps by way of some rubbing and scrubbing, and disposed of
} (or better yet, recycled) and the refrigerator disposed of as any ordinary
} refrigerator might be dealt with?
}

The problem is that any form of osmium is a heavy metal, and therefore
should be treated as one would cadmium or arsenic. It is not innocuous.

Don
}

______________________________________________________________________
Donald L. Lovett e-mail: lovett-at-tcnj.edu
Assoc. Professor, Dept. of Biology voice: (609) 771-2876
P.O. Box 7718 fax: (609) 637-5118
The College of New Jersey
Ewing, NJ 08628-0718





From daemon Thu Jun 22 08:42:43 2000



From: yliu-at-unlserve.unl.edu (Yi Liu)
Date: Thu, 22 Jun 2000 08:38:21 -0500
Subject: Need info on magnetic structure characterization

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Dear Colleagues:

We have made magnetic nanodots and metastable magnetic phases. The
physical structures are well revealed by TEM. However, we would like to
relate the magnetic properties with magnetic structure and physical
structure. Are there other methods such as neutron diffraction, X-ray or
spin polarized electrons that can characterize the magnetic
structure/ordering. Please contact me if you could offer such
info/collaboration. Thanks a lot.

Yi

*******************************************************************
Yi Liu
Department of Mechanical Eng. and CMRA
104 N Walter Scott Engineering Center
University of Nebraska-Lincoln
Lincoln, NE 68588-0656
Tel. (402) 472-7759 (Office)
Tel. (402) 472-8762 (EM lab)
Fax (402) 472-1465
Email: yliu-at-unlserve.unl.edu
*******************************************************************




From daemon Thu Jun 22 20:19:13 2000



From: Anthony Garratt-Reed :      tonygr-at-mit.edu
Date: Thu, 22 Jun 2000 10:00:13 -0400
Subject: Re: Interface Analysis

Contents Retrieved from Microscopy Listserver Archives
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YinHsian -

This is a very complex problem. You don't mention the thickness of the
intermetallic layers. EDX analysis has relatively poor spatial resolution
in the SEM (of the order of microns) so you can clearly see features that
you cannot analyze well. People often use backscatter electron imaging
(which can be calibrated) in cases such as yours. You should read a good
book on SEM - the subject is too complex to cover in an e-mail like this.

If you can make thin foils of the sample, AEM (analytical electron
microscopy) analysis would overcome most of the problems. A LaB6
instrument will give you better than 10 nm analytical resolution, while a
field-emitter will approach 1 nm resolution. The problem, though, will be
specimen preparation. I would be tempted to suggest using a microtome to
cut thin slices, but I don't know the form of your material.

Good luck!

Tony Garratt-Reed


At 06:37 PM 06/22/2000 +0800, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

** Anthony J. Garratt-Reed
** MIT Room # 13-1027
** 77 Massachusetts Avenue
** Cambridge, MA 02139-4307
** USA
**
** Phone: (+) 1-617-253-4622
** Fax: (+) 1-617-258-6479
**




From daemon Thu Jun 22 20:19:16 2000



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Thu, 22 Jun 2000 09:02:05 -0700
Subject: Re: chemical reference standards for the analysis of a Ni-base

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Dear Paul-Laurent,
The usual standards that are used for alloy analysis in the SEM+EDX are pure
element standards. You can prepare pure (three or four nines, e.g. 99.9 or
99.99%) metal standards yourself, or any of the EM catalog houses will list
standards consisting of pure metals or minerals covering the range of most
common elements. They are fairly expensive (~$2000US) and I am not sure of
delivery time. Tousimis is one standard supplier that I know of. The same
standards are used for EDX or WDX analysis. You collect the spectra from
these standards under carefully reproducible conditions in your SEM and
follow the instructions in your EDX software to do fully standard analysis
with matrix corrections. You can store the standard spectra in the EDX
computer, so you only need to run them once for each set of conditions.
It is quite difficult to get an accurate and precise analysis of Al in Ni
alloys, because there is a large correction factor for absorption of the Al
x-rays by the Ni matrix, and because the best SEM conditions for Ni
analysis, 20 kV, is a large overvoltage for Al. It is very helpful if you
can make or obtain a well-characterized, homogeneous alloy in the range of
the alloy you will be testing, so you can check the results of your EDX
analysis. The last time I did a careful phase analysis of an alloy
consisting of 5% Al in Zn, I eventually went to analysing the Al at 10 kV
and doing the Zn by difference. This was the only way to get the Al results
with the accuracy required and agreeing with the phase diagram. With the
other elements present in your system, you cannot use the
element-by-difference method, but you could check to see if you get the same
Al results at 10 kV, doing the Ni by difference (forgetting the other
elements), as you get in your full, standard analysis at 20 kV.
Most of the EDX supplier companies have excellent instruction books that
cover the topics of chemical analysis by EDX and the exact conditions
required for accurate analysis. Some offer courses in EDX analysis and they
are a very good way to get familiar with the strengths and weaknesses of the
system.
Please contact me if you need any more information.
At 07:07 PM 6/21/00 -0500, you wrote:

} CAN SOMEBODY HELP ME?:
}
} -We need chemical reference standards for the analysis of a Ni-base alloy.
}
} -We intend to use the energy-dispersive X-ray analysis, coupled to a
} Scanning
} Electron Microscope(SEM).
}
} -The composition of the alloy is going to vary between 18 to 28 wt%
} Aluminium ;
} Al is the most important element to be tested, the remainder of the alloy
} being
} mostly Ni of course.
}
} -Secondary main elements are : Cr from 4 to 10 wt% ; Co from 10 to 15
} wt% ; Elements in low concentration (below 3%) are Ti and Mo.
}
} -Accuracy : 0.5 % in Al is required.
}
}
} Questions are :
}
} -How many different standards would we need ?
}
} -What should be the chemical variations from standard to standard to reach
} the
} required accuracy ?
}
} -What would be the price for such standards ? Time for delivery ? Where to
} order this ?
}
} -Can you send us information (reprints from technical journals, articles) on
} the
} topic of chemical analysis by means of energy-dispersive (or
} wavelength-dispersive) X-ray systems ?
}
} If you need any further information, please contact me.
}
} In anticipation, many thanks for a prompt answer.
}
} Yours faithfully,
}
} PAUL-LAURENT CAPRON JR.

Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Thu Jun 22 20:19:19 2000



From: Tom Januszewski :      tom.januszewski-at-email.swmed.edu
Date: Thu, 22 Jun 2000 12:57:41 -0500
Subject: Critical Point Dryers

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Hi Listservers,
We are in the process of replacing our old
critical point dryer and have narrowed our
selection to the following choices: Tousimis
Samdri 795 semi automatic and the EMS 850
critical point dryers.
If anyone out there is familiar with either
of these instruments and would care to
comment on their advantages and
disadvantages, I would appreciate hearing
from you. Offline replies are welcome. I
will report back with summary of results.

Tom Januszewski
Senior Electron Microscopist
Molecular and Cellular Imaging Facility
UT Southwestern Medical Center at Dallas
5323 Harry Hines Blvd.
Dallas, TX 75390-9039
Phone: 214-648-7291
Fax: 214-648-6408
Email: tom.januszewski-at-email.swmed.edu



From daemon Thu Jun 22 20:19:24 2000



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Thu, 22 Jun 2000 13:37:42 -0700
Subject: Re: JVG-N2 vacuum gauge and forgel tubes

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Dear Kristian,
I also have an old JEOL evaporator (JEE-4B) and, yes, the Forgel tube was
just a hot-wire ionization gauge. I got tired of buying new ones, so I
successfullly replaced that with a cold cathode gauge attached to a tube of the
right diameter.
At 01:54 PM 6/22/00 +0300, you wrote:
} Hello,
}
} I'm looking for a manual for the JVG-N2 ionization
} vacuum gauge. I've already tried JEOL and they could
} find JVG-N1 manual (thanks!), but not -N2, and -N1 and
} -N2 are different enough (different connectors, apparently
} EB grid heating in -N2 etc.) that the -N1 manual is not
} enough to get the gauge operational.
}
} So, anyone with manual for JVG-N2 ??
}
} btw: The JVG-N1 manual mentions "Forgel tube" which looks
} in drawing just like normal hot-filament ionization
} gauge tube.. Is "Forgel tube" just an old term for one?
}
} Thanks,
}
} Kristian Ukkonen.
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Thu Jun 22 20:19:25 2000



From: Chris Jeffree :      cjeffree-at-srv0.bio.ed.ac.uk
Date: Thu, 22 Jun 2000 21:57:57 +0100
Subject: Re: Refrigerator osmium

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


This doesn't seem to cover it. Gold and platinum are heavy metals.
So are we to treat them as we treat osmium tetroxide?
Where does light end and heavy begin anyway? There is a real
need here for a proper definition of the risk.

}
} The problem is that any form of osmium is a heavy metal, and therefore
} should be treated as one would cadmium or arsenic. It is not innocuous.
}
} Don
} }
}
} ______________________________________________________________________
} Donald L. Lovett e-mail: lovett-at-tcnj.edu
} Assoc. Professor, Dept. of Biology voice: (609) 771-2876
} P.O. Box 7718 fax: (609) 637-5118
} The College of New Jersey
} Ewing, NJ 08628-0718
}
}
}
}


~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Dr Chris Jeffree
University of Edinburgh
Biological Sciences EM Facility
Daniel Rutherford Building
King's Buildings EDINBURGH EH9 3JH
Tel: +44 (0) 131 650 5345
FAX: +44 (0) 131 650 6563

Inveresk Cottage, 26 Carberry Road,
Inveresk, Musselburgh, Midlothian EH21 8PR, UK
Tel. +44 (0) 131 665 6062 / Mobile 0410 585 401
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~



From daemon Thu Jun 22 20:19:27 2000



From: Corazon Bucana :      bucana-at-audumla.mdacc.tmc.edu
Date: Thu, 22 Jun 2000 16:18:36 -0500
Subject: EM autoradiography

Contents Retrieved from Microscopy Listserver Archives
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I received a request for EM autoradiography of C14 labeled polymers. The
idea is to trace the distribution of the polymer once it is internalized in
the cell. It has been a long while since I have done autoradiography and I
was wondering if there are new books and information that is available out
there. Is anyone still using this technique. What are the alternatives? I
am not too keen on handling radioactive materials again. I would appreciate
comments.

*******************************************************
Corazon D. Bucana, Ph.D.
Department of Cancer Biology
U.T. M.D. Anderson Cancer Center
1515 Holcombe Blvd. Box 173
Houston, Texas 77030
Phone: (713) 792-8106
FAX: (713) 792-8747
Email:bucana-at-audumla.mdacc.tmc.edu
FAX: (713) 792-8747



From daemon Thu Jun 22 20:19:28 2000



From: Ken Long :      long-at-ROBLES.CALLUTHERAN.EDU
Date: Thu, 22 Jun 2000 15:05:00 -0700
Subject: undergrad SEM use

Contents Retrieved from Microscopy Listserver Archives
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Greetings:
I am a new subscriber to this list and am looking for input/advice
regarding the use of SEM's in undergraduate research. I teach at a liberal
arts university with only undergraduate programs in the natural sciences.
A geology colleague and myself (I'm a biologist) are exploring possible
funding sources which would allow us to purchase an SEM that would be used
by biology and geology undergrads in their independent and senior thesis
projects. (We currently have a non-functioning ETEC that we would like to
replace -- we've been advised that further repair and head-banging would
not be cost effective). If anyone is currently engaged in SEM-based
research with undergrads, I would like to get your feedback on your
experiences (both the pluses and minuses). Although we don't have a TEM,
input from those using TEM with undergrads would also be helpful.

Specific Questions:

1) What types of projects are your students pursuing?

2) Are you conducting interdisciplinary projects? e.g., micropaleontology
or environmental applications.

3) Are there particular models of SEMs which would be more user friendly
since we would be training undergrads and we would also be the primary
trainers and technicians?

4) Have you developed an undergrad EM course?

Thanks,
Ken

-----------------------------------------------------------------------
Kenneth Long, PhD email: long-at-clunet.edu
Associate Professor, Biology Office: (805) 493-3346
California Lutheran University Fax: (805) 493-3392
60 West Olsen Rd. #3700 http://www.clunet.edu/Biology/Anatomy
Thousand Oaks, CA 91360




From daemon Thu Jun 22 20:19:28 2000



From: Debby Sherman :      sherman-at-btny.purdue.edu
Date: 22 Jun 2000 17:11:55 -0500
Subject: M&M-Facility Management

Contents Retrieved from Microscopy Listserver Archives
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------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Fellow Microscopists:
Stuart Mckernan,M&M'00 Program Chair, has arranged for an additional "EXPERTS" session for the topic of Facility Management. This special session will be held on Wednesday, August 16 at 9:00Am in Room 106. Advertising will be done by flyers, daily news bulletin, and word of mouth.

The room will be available all morning so that we can be somewhat flexible in timing. However, in order to try to make the session as constructive as possible, I would like to identify 2-3 main topics of interest to lab managers from both academic and industrial facilities. These topics will be covered first. Additional topics can then be discussed based on interest and time.

I would appreciate it if those who are interested in attending this session would provide the following:
a) 2-3 topics of primary interest to you ranked in order of that interest.
b) suggestions of individuals who might be asked to introduce a specific topic so as to give a framework for further discussion.

I am heading out for vacation on July 1 and will unsubscribe from the list a few days prior to then. Please send the above information directly to me rather than to the list so I am sure to get it. We will announce the topics to be discussed and approximate times they will be discussed so that you can choose when you want to attend the session.

If the session is well attended, then it may be added to the agendas of future meeting. Hope to hear from many of you regarding the topics.
Debby

Debby Sherman, Manager Phone: 765-494-6666
Microscopy Center in Agriculture FAX: 765-494-5896
Dept. of Botany & Plant Pathology E-mail: sherman-at-btny.purdue.edu
Purdue University
1057 Whistler Building
West Lafayette, IN 47907-1057



From daemon Thu Jun 22 20:19:29 2000



From: E. J. McKenzie :      elizm-at-pdx.edu
Date: Thu, 22 Jun 2000 15:50:11 -0700
Subject: Formaldehyde problems

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Hi everyone,

I'm currently making 1.5 litre batches of 4% formaldehyde
using paraformaldehyde, sodium hydroxide and distilled water and not
having much success. The mixture immediately begins to polymerize. The
recipe I used is simply an amplified version of Karnovsky's recipe for 25ml
of 4% (which works perfectly). I am wondering whether my assumption of a
simple linear relationship between the proportions of NaOH and
paraformaldehyde is incorrect. Making stock solutions of higher
concentrations
does not work either.

Does anyone know a more successful method of preparing large
volumes of formaldehyde? We do not want to use phosphates or
preservatives.

regards
Elizabeth McKenzie
--------------------------------------------------
Geomicrobiology and Electron Microscopy Laboratory
Room S9 Cramer Hall
1721 SW Broadway
Portland State University
Portland
OR97201

ph:503 725 3362
fax:503 725 3025



From daemon Thu Jun 22 21:20:34 2000



From: Barry Searle :      B.Searle-at-unsw.edu.au
Date: Thu, 22 Jun 2000 21:10:45 -0500
Subject: JEE 4B/4C Coater

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Fellow EM Users, Would anyone have a list of the O rings for use on
the JEOL JEE4B/4C vacuum evaporator. The photocopy of pages from the
manual that I have does not list any o-rings. Thankyou Barry EM UNIT
UNSW




From daemon Fri Jun 23 08:04:52 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Thu, 22 Jun 2000 21:07:37 -0700
Subject: Fwd: Polaron E5200 control board

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


One response. No follow through. Board is going
into the circular file.

gg



} Date: Thu, 15 Jun 2000 07:55:38 -0700
} To: MSA listserver
} From: "Dr. Gary Gaugler" {gary-at-gaugler.com}
} Subject: Polaron E5200 control board
}
} I have a control PC board for the E5200 sputter coater.
} This is the model with an Intel single chip MPU on one
} end and a 4-conductor socket on the other end. The
} board uses a VME connector for main interface.
}
} Coater is trashed. Board is OK. If anybody can use
} the board, first request gets it.
}
} gary g.



From daemon Fri Jun 23 08:04:53 2000



From: jim :      jim-at-proscitech.com.au
Date: Fri, 23 Jun 2000 14:48:14 +1000
Subject: RE: Refrigerator osmium

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I had a look at the excellent site
http://www.webelements.com/index.html

clicking on osmium and then asking for "biology" this info is given:
"Osmium metal does not normally cause problems as it is relatively unreactive
but all osmium compounds should be regarded as highly toxic. The metal dust is
an irritant and presents a fire and explosion hazard. Osmium oxide, OsO4, is
highly toxic, and boils at 130?C (760 mm). Concentrations in air as low as 10-7
g m-3 can cause lung congestion, skin damage, and severe eye damage. The oxide,
in particular, should only ever be handled by a properly qualified chemist."
We all know about the tetroxide, but the point I made in my previous email was
that the fridge contamination is Osmium metal and this has little reactivity.
Osmium when finely dispersed would produce a tiny amount of tetroxide. Possibly
not enough to worry while in the lab, certainly not if its in landfill or
smelted.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
www.proscitech.com

On Friday, June 23, 2000 6:58 AM, Chris Jeffree
[SMTP:cjeffree-at-srv0.bio.ed.ac.uk] wrote:}
}
} This doesn't seem to cover it. Gold and platinum are heavy metals.
} So are we to treat them as we treat osmium tetroxide?
} Where does light end and heavy begin anyway? There is a real
} need here for a proper definition of the risk.
}
} }
} } The problem is that any form of osmium is a heavy metal, and therefore
} } should be treated as one would cadmium or arsenic. It is not innocuous.
} }
} } Don
} } }
} }
} } ______________________________________________________________________
} } Donald L. Lovett e-mail: lovett-at-tcnj.edu
} } Assoc. Professor, Dept. of Biology voice: (609) 771-2876
} } P.O. Box 7718 fax: (609) 637-5118
} } The College of New Jersey
} } Ewing, NJ 08628-0718
} }
} }
} }
} }
}
}
} ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
} Dr Chris Jeffree
} University of Edinburgh
} Biological Sciences EM Facility
} Daniel Rutherford Building
} King's Buildings EDINBURGH EH9 3JH
} Tel: +44 (0) 131 650 5345
} FAX: +44 (0) 131 650 6563
}
} Inveresk Cottage, 26 Carberry Road,
} Inveresk, Musselburgh, Midlothian EH21 8PR, UK
} Tel. +44 (0) 131 665 6062 / Mobile 0410 585 401
} ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~



From daemon Fri Jun 23 08:05:00 2000



From: Oldrich Benada :      benada-at-biomed.cas.cz
Date: Fri, 23 Jun 2000 11:11:15 +0200
Subject: A hole in the EDX spectrum II

Contents Retrieved from Microscopy Listserver Archives
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Dear colleagues,
Many thanks for all responses to my question considering the hole in
the EDX spectrum.
We had a service engineer in our lab and he tried to solve the
problem but he was not successful.
I have prepared WEB page with the images of spectra, that were
recorded from following samples:
Cu_Al sample (usually used for calibration)
Ti grid
AL grid
K standard

The url of that page is following:
http://www.biomed.cas.cz/~benada/EDX_page/index.htm

Please, if anyone would be so kind and could comment the spectra, we
will be very happy.
Best regards from Prague
Oldrich Benada


+-----------------------------------+
Oldrich Benada
Acad. Sci. CR
Institute of Microbiology
Laboratory of electron microscopy
Videnska 1083
CZ - 142 20 Prague 4 - Krc
Czech Republic
+------------------------------------+
Phone: +420-2-4752399
Fax: +420-2-4715743
WEB:http://www.biomed.cas.cz/mbu/lem113/lem.htm



From daemon Fri Jun 23 08:05:01 2000



From: Bart De Pauw :      Bart.DePauw-at-rug.ac.be
Date: Fri, 23 Jun 2000 11:42:12 +0200
Subject: Re: Formaldehyde problems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


There's nothing mysterious about this. It should be possible to
make any volume of formaldehyde solution over a wide range of
concentrations. We routinely make 10% solutions without
problems in volumes much greater than 25ml. There is no reason
to suppose that the volume is relevant, and the concentration is not
critical.
The temperature needs to be } 60oC and the solution needs to be
slightly alkaline. Otherwise it is extremely straightforward.
Could you tell us your procedure, step by step? Then maybe it will
be possible to diagnose the problem.
Chris


Date sent: Thu, 22 Jun 2000 15:50:11 -0700


Commerially available formaldehyde contains formic acid and methanol and is
therefore unsuitable for EM. Instead, one usually prepares a solution of
formaldehyde from paraformaldehyde. An aqueous solution (40%, w/v) can be made
by warming for 1 h at 65¡V (constant stirring). Milkyness can be overcome by
adding a few drops of a 40% (w/v) NaOH solution. This solution is stable for
several weeks at 4¡c (in the dark).
Hopes this helps.

De Pauw Bart
Ghent University
Faculty of Veterinary Medicine
Department Morphology


"E. J. McKenzie" schreef:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hi everyone,
}
} I'm currently making 1.5 litre batches of 4% formaldehyde
} using paraformaldehyde, sodium hydroxide and distilled water and not
} having much success. The mixture immediately begins to polymerize. The
} recipe I used is simply an amplified version of Karnovsky's recipe for 25ml
} of 4% (which works perfectly). I am wondering whether my assumption of a
} simple linear relationship between the proportions of NaOH and
} paraformaldehyde is incorrect. Making stock solutions of higher
} concentrations
} does not work either.
}
} Does anyone know a more successful method of preparing large
} volumes of formaldehyde? We do not want to use phosphates or
} preservatives.
}
} regards
} Elizabeth McKenzie
} --------------------------------------------------
} Geomicrobiology and Electron Microscopy Laboratory
} Room S9 Cramer Hall
} 1721 SW Broadway
} Portland State University
} Portland
} OR97201
}
} ph:503 725 3362
} fax:503 725 3025



From daemon Fri Jun 23 08:05:02 2000



From: Frank Thomas :      thomasf-at-AGC.BIO.NS.CA
Date: Fri, 23 Jun 2000 07:58:40 -0300
Subject: Strontium-based seashells?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Here's an interesting problem. The other day I was examining some plankton
trap samples in our ESEM, and found a few bits of what I took to be pieces
of foraminiferal shell - they were slightly curved, crystalline-looking
shards with closely spaced fine openings (the foramen, for which forams are
named) and a few large holes (possible spine attachment localities). I ran
EDS on them to make sure that is indeed, what they were. Planktic foram
shells should be fairly pure calcium carbonate. What I got instead were
spectra each showing a fairly large strontium peak, a smaller sulfur peak
and a little oxygen. There were also very high aluminum peaks on each one,
but since the sample was simply air dried on an aluminum stub, I assume
that was the source of the Al.
In 20-some years of micropaleontology I've never heard of any marine
organism that uses strontium to build its shell. Sr is just below Ca in the
periodic table, so I suppose it shares some of the same reactability
characteristics (but I'm no chemist), so perhaps it could substitute, if no
Ca was available (which would be darn funny in a surface marine sample).
Let me add that all the other peaks were in their proper places, and the
instrument had been calibrated quite recently, and I can think of no source
of strontium contamination which could have gotten in there. We're planning
on sending some of these bits out for analysis by some other means just to
make sure, but for the time being, it's quite a mystery.
Any ideas from the marine biologists? The chemists? The EDS gurus?
Psychics?


F.C. Thomas
MicroAnalysis Facility
Geological Survey of Canada (Atlantic)
Bedford Institute of Oceanography
Dartmouth, Nova Scotia
Canada B2Y 4A2



From daemon Fri Jun 23 08:05:04 2000



From: Thomas A. Baginski :      tombg-at-bictom.usuf1.usuhs.mil
Date: Fri, 23 Jun 2000 08:09:10 -0400
Subject: ***TEM Darkroom Users*** AGFA Paper has become extinct !

Contents Retrieved from Microscopy Listserver Archives
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We use the Agfa DD3700 paper processor. However, the wonderful
graded papers are no longer available and have not been for some time now.
Our back-up system is the Mohr Pro-8. Most of our older trained users are
unwilling to use the AGFA multi-contrast graded papers, stating that the
iamge is "not as crisp, not as white, harder to work with", etc.
If anyone has used AGFA graded papers in the past, and has found a
resin-coated graded paper alternative that works to give sharp, crisp, clean,
white, micrographs from the DURST Point source, I would like to hear about
your solutions.
Some un-tried recommendations have been: FOMA papers, FORTE papers,
ILFORD papers, and then there is the rest of the list, EFKE, Kentmere,
Kodak, MACO, Oriental, adn ECCO. Does anyone know if there is a graded
paper comparable to the AGFA RAPITONE GRADES P1,P2, P 3, and P4. AGFA
only suggests to use their polycontrast resin papers.
I have swithced to digital, but the older folks with all the great
publications refuse to lower their standards.
I would lke to know more about how I can get the wanted details
back onto my micrographs. ANY IDEAS ? ....-TOM

Thomas A Baginski
Technical Coordinator for Microscopy
Uniformed Services University of the Health Sciences
Bethesda, MD 20814-4799

Voice Phone: 301 295 5691
Fax: 301 319 8218
Email: tbaginski-at-usuhs.mil



From daemon Fri Jun 23 08:05:06 2000



From: Roger Mason :      rmason-at-sparky2.esd.mun.ca
Date: Fri, 23 Jun 2000 09:55:58 -0230
Subject: Re: Strontium-based seashells?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Frank,

Depending on the polymorph of CaCO3 that foram shells are made of, a few hundred
(calcite) to a few thousand (aragonite) ppm of Sr would not be unusual in carbonate
precipitated from standard sea-water. SO4 ion is also a fairly significant component
of sea-water and S can be incorporated in the structures of carbonate minerals in
small amounts. It could also be present as fluid inclusions. Another possibility is
that the seawater present in the pores of the forams on recovery from the ocean has
precipitated celestite (SrSO4) on dessication, in which case the Sr might have a
very patchy distribution.

Roger Mason

Frank Thomas wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Here's an interesting problem. The other day I was examining some plankton
} trap samples in our ESEM, and found a few bits of what I took to be pieces
} of foraminiferal shell - they were slightly curved, crystalline-looking
} shards with closely spaced fine openings (the foramen, for which forams are
} named) and a few large holes (possible spine attachment localities). I ran
} EDS on them to make sure that is indeed, what they were. Planktic foram
} shells should be fairly pure calcium carbonate. What I got instead were
} spectra each showing a fairly large strontium peak, a smaller sulfur peak
} and a little oxygen. There were also very high aluminum peaks on each one,
} but since the sample was simply air dried on an aluminum stub, I assume
} that was the source of the Al.
}



From daemon Fri Jun 23 08:05:06 2000



From: Geoff McAuliffe :      mcauliff-at-UMDNJ.EDU
Date: Fri, 23 Jun 2000 08:30:54 -0400
Subject: Re: ***TEM Darkroom Users*** AGFA Paper has become extinct !

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


"Thomas A. Baginski" wrote:

} We use the Agfa DD3700 paper processor. However, the wonderful
} graded papers are no longer available and have not been for some time now.
} Our back-up system is the Mohr Pro-8. Most of our older trained users are
} unwilling to use the AGFA multi-contrast graded papers, stating that the
} iamge is "not as crisp, not as white, harder to work with", etc.
} If anyone has used AGFA graded papers in the past, and has found a
} resin-coated graded paper alternative that works to give sharp, crisp, clean,
} white, micrographs from the DURST Point source, I would like to hear about
} your solutions.
} Some un-tried recommendations have been: FOMA papers, FORTE papers,
} ILFORD papers, and then there is the rest of the list, EFKE, Kentmere,
} Kodak, MACO, Oriental, adn ECCO. Does anyone know if there is a graded
} paper comparable to the AGFA RAPITONE GRADES P1,P2, P 3, and P4. AGFA
} only suggests to use their polycontrast resin papers.
} I have swithced to digital, but the older folks with all the great
} publications refuse to lower their standards.
} I would lke to know more about how I can get the wanted details
} back onto my micrographs. ANY IDEAS ? ....-TOM
}
} Thomas A Baginski
} Technical Coordinator for Microscopy
} Uniformed Services University of the Health Sciences
} Bethesda, MD 20814-4799
}
} Voice Phone: 301 295 5691
} Fax: 301 319 8218
} Email: tbaginski-at-usuhs.mil

Dear Tom:

I have had good results with both Kodak and Ilford multigrade resin coated
papers in my DD-3700. I am surprised that you can see a difference in sharpness
between modern. glossy papers. Is Kodabrom RC still available in grades?

Geoff
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************




From daemon Fri Jun 23 08:05:06 2000



From: donald j marshall :      dmrelion-at-world.std.com
Date: Fri, 23 Jun 2000 08:42:39 -0400 (EDT)
Subject: Re: Strontium-bases sea shells

Contents Retrieved from Microscopy Listserver Archives
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Frank, I am not a microbiologist or even close - I work in
cathodoluminescence instrumentation. I recalled that Sheldon Sommer made
some comments on Sr in shells in his work at Penn State. On page 282 of his
paper, he talks about 70 mole percent strontianite in pelecypods, for what
it is worth. Sommer, S. E. CL of carbonates,2. Geological Applications.
Chemical geology, 1972, pp. 275-284.

Don Marshall


Donald J. Marshall
Relion Industries
P.O. Box 12
Bedford, MA 01730
Ph: 781-275-4695
FAX: 781-271-0252
email dmrelion-at-world.std.com

Cathodoluminescence, mass spectroscopy, electron beam technology


"A weed is a flower out of place."

(Please note: Do not send email with attachments to this address. Instead, send it to donbarlen-at-aol.com. Thank you.)



From daemon Fri Jun 23 08:15:56 2000



From: A.P. Alves de Matos :      apmatos-at-ip.pt
Date: Fri, 23 Jun 2000 14:17:25 +0100
Subject: Stainning neurosecretory cells

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear all

I just found some cells with granules resembling neurosecretory granules in
invertebrate tissues, and I need to confirm their nature.

Can anyone recomend a straightforward and reliable method to identify
neurosecretory cells (at the light or/and electron microscopic levels)?


Thanks to all
Dr. A.P. Alves de Matos





From daemon Fri Jun 23 08:15:56 2000



From: Philip Oshel :      peoshel-at-facstaff.wisc.edu
Date: Fri, 23 Jun 2000 08:18:36 -0500
Subject: Re: Strontium-based seashells?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


There is a group of marine protistans that uses strontium sulphate to
make their tests. Amoeboid critters closely related to ... wait a bit
while I dredge through my memory here, my texts are at home ...
Actinaria? Relatives of the Radiolarians and Heliozoa IF I'm
remembering correctly. They're mentioned inter alia in the book
"Synoptic Classification of Living Organisms" and any good
invertebrate zoology text or Protistology text.

Phil
The Al is most likely from the stub ... try a carbon mount.

} Here's an interesting problem. The other day I was examining
} some plankton
} trap samples in our ESEM, and found a few bits of what I took to be pieces
} of foraminiferal shell - they were slightly curved, crystalline-looking
} shards with closely spaced fine openings (the foramen, for which forams are
} named) and a few large holes (possible spine attachment localities). I ran
} EDS on them to make sure that is indeed, what they were. Planktic foram
} shells should be fairly pure calcium carbonate. What I got instead were
} spectra each showing a fairly large strontium peak, a smaller sulfur peak
} and a little oxygen. There were also very high aluminum peaks on each one,
} but since the sample was simply air dried on an aluminum stub, I assume
} that was the source of the Al.
} In 20-some years of micropaleontology I've never heard of any marine
} organism that uses strontium to build its shell. Sr is just below Ca in the
} periodic table, so I suppose it shares some of the same reactability
} characteristics (but I'm no chemist), so perhaps it could substitute, if no
} Ca was available (which would be darn funny in a surface marine sample).
} Let me add that all the other peaks were in their proper
} places, and the
} instrument had been calibrated quite recently, and I can think of no source
} of strontium contamination which could have gotten in there. We're planning
} on sending some of these bits out for analysis by some other means just to
} make sure, but for the time being, it's quite a mystery.
} Any ideas from the marine biologists? The chemists? The EDS gurus?
} Psychics?
}
}
} F.C. Thomas
} MicroAnalysis Facility
} Geological Survey of Canada (Atlantic)
} Bedford Institute of Oceanography
} Dartmouth, Nova Scotia
} Canada B2Y 4A2

--
}}}}}}}}}}}}}}}}}{{{{{{{{{{{{{{{{{
Philip Oshel
Supervisor, AMFSC and BBPIC
Dept. of Animal Health and Biomedical Sciences
University of Wisconsin
1656 Linden Drive
Madison, WI 53706-1581
voice: (608) 263-4162
fax: (608) 262-7420 (dept. fax)



From daemon Fri Jun 23 08:27:31 2000



From: barbarac-at-biols.susx.ac.uk ()
Date: Fri, 23 Jun 2000 08:22:55 -0500
Subject: Ask-A-Microscopist:Staining Question

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form. It was submitted by
(barbarac-at-biols.susx.ac.uk) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Friday, June 23,
2000 at 08:00:37
---------------------------------------------------------------------------

Email: barbarac-at-biols.susx.ac.uk
Name: Barbara Ciani
School: University of Sussex
Question: Hi everyone,

I need to do some congo red staining on my peptides and
i need to make sure they stick on the microscope slides.

I have been suggested to use gelatine with chrome alum but my slides don't
seem to get 'sticky'.

Any suggestion?

I use 0.5% gelatine (300 bloom) and 0.05% chrome alum.

Thanks,

Barbara

---------------------------------------------------------------------------




From daemon Fri Jun 23 08:37:04 2000



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Fri, 23 Jun 2000 08:39:32 -0500
Subject: ***TEM Darkroom Users*** AGFA Paper has become extinct !

Contents Retrieved from Microscopy Listserver Archives
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Date sent: Mon, 19 Jun 2000 09:46:49 -0400
To: {Microscopy-at-sparc5.microscopy.com}
} From: Leona Cohen-Gould {lcgould-at-mail.med.cornell.edu}


Tom,

Although we do very little photographic printing anymore, I printed
extensively in the past and have gotten excellent results from Kodak and
Ilford multi-contrast resin-coated papers, using a Durst professional
enlarger, as well as Beseler enlargers of various types. What I have never
done, though, is make much use of a paper processor. Force of habit, maybe,
but I always preferred tray processing, even when I had to make lots of
prints in short amounts of time.

Even resin-coated papers respond somewhat to variations in developing
times/temperatures, etc., allowing minor adjustments during processing.
Often it is possible to watch the image come up in the tray and know within
15-30 seconds whether it will acceptable or what changes will be necessary.
I can only speak for myself, but quality-wise I believe that tray processing
is superior, in terms of tonality, grey-scale range, etc. I also believe
it's as fast or faster.

That said, I know that people accustomed to processors are just as stubborn
as we dinosaurs who prefer tray processing, and nobody is likely to change
long-established habits. Any mainstream resin-coated, glossy paper should
capture any needed detail that's present in the negative. I'm not saying
that photo paper is as "sharp" in terms of lines/mm as a TEM negative, but
only that the detail it won't capture is too small to see in normal viewing,
and certainly won't survive the reproduction process in a journal. If
you're visibly losing detail in the print at a standard, or even close,
viewing distance without a lens, I would be checking the negatives and
enlarger.

I won't even get into the debate about journals considering digital imaging
as a "lowering of standards"! Not going there... :-)

Just my very subjective two cents.

All the best,
Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/



-----Original Message-----
} From: Thomas A. Baginski [mailto:tombg-at-bictom.usuf1.usuhs.mil]
Sent: Friday, June 23, 2000 7:09 AM
To: Microscopy-at-sparc5.microscopy.com


We use the Agfa DD3700 paper processor. However, the wonderful
graded papers are no longer available and have not been for some time now.

Our back-up system is the Mohr Pro-8. Most of our older trained users are

unwilling to use the AGFA multi-contrast graded papers, stating that the
iamge is "not as crisp, not as white, harder to work with", etc.
If anyone has used AGFA graded papers in the past, and has found a
resin-coated graded paper alternative that works to give sharp, crisp,
clean,
white, micrographs from the DURST Point source, I would like to hear about

your solutions.
Some un-tried recommendations have been: FOMA papers, FORTE papers,

ILFORD papers, and then there is the rest of the list, EFKE, Kentmere,
Kodak, MACO, Oriental, adn ECCO. Does anyone know if there is a graded
paper comparable to the AGFA RAPITONE GRADES P1,P2, P 3, and P4. AGFA
only suggests to use their polycontrast resin papers.
I have swithced to digital, but the older folks with all the great
publications refuse to lower their standards.
I would lke to know more about how I can get the wanted details
back onto my micrographs. ANY IDEAS ? ....-TOM

Thomas A Baginski
Technical Coordinator for Microscopy
Uniformed Services University of the Health Sciences
Bethesda, MD 20814-4799

Voice Phone: 301 295 5691
Fax: 301 319 8218
Email: tbaginski-at-usuhs.mil



From daemon Fri Jun 23 08:55:07 2000



From: Geoff McAuliffe :      mcauliff-at-UMDNJ.EDU
Date: Fri, 23 Jun 2000 09:46:22 -0400
Subject: Re: Stainning neurosecretory cells

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


"A.P. Alves de Matos" wrote:

} Dear all
}
} I just found some cells with granules resembling neurosecretory granules in
} invertebrate tissues, and I need to confirm their nature.
}
} Can anyone recomend a straightforward and reliable method to identify
} neurosecretory cells (at the light or/and electron microscopic levels)?
}
} Thanks to all
} Dr. A.P. Alves de Matos

Chrome-alum hematoxylin or Falhmi's aldehyde fuchsin work well with mammalian
tissues. Check almost any edition of Humason's "Animal Tissue Techniques" for
specifics. Modifications for invert tissues are out there but I don't have
references handy.

Geoff
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************




From daemon Fri Jun 23 18:27:54 2000



From: Mike Coviello :      coviello-at-mae.uta.edu
Date: Fri, 23 Jun 2000 09:23:02 -0500
Subject: Re: TEM-Indepedent FIB lab-a thank you

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Y'all:
Thanks for your overwelming response to my request for an independent
FIB lab that can do TEM x-sections. Over the years, this forum has been
an invaluable resource for me.
Regards, Mike Coviello
Lab Manager
University of Texas -at- Arlington



From daemon Fri Jun 23 18:27:56 2000



From: Belluso elena :      belluso-at-dsmp.unito.it
Date: Fri, 23 Jun 2000 16:44:22 +0200 (MDT)
Subject: Need mineralogical samples for TEM studies

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello everyone,
I'm studying microstructures and intergrowths of fibrous minerals by TEM.
I'm looking for a sample of amosite and a sample of antophillite fibres in a
rock matrix in order to continue this study. I'm gratefully if someone will
send my these samples and will be interested to collaborate.

Thank you everyone,
Elena Belluso



----------------------------------------------------
Elena BELLUSO
Dipartimento di Scienze Mineralogiche e Petrologiche
Via Valperga Caluso, 35
I-10125 TORINO - ITALIA
tel:(39) 011.670.71.35 - fax: (39) 011.670.71.28
e-mail: belluso-at-dsmp.unito.it
http://www.dsmp.unito.it
----------------------------------------------------



From daemon Fri Jun 23 18:27:56 2000



From: tracy gales :      tl_gales-at-fccc.edu
Date: Fri, 23 Jun 2000 11:01:58 -0400
Subject: Please unsubscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




--
Tracy Gales
Fox Chase Cancer Center
Electron Microscopy Facility
7701 Burholme Ave.
Philadelphia PA 19111
Tel. 215-728-2484
Fax 215-728-2412




From daemon Fri Jun 23 18:27:57 2000



From: Stuart McKernan :      mckernan-at-cems.umn.edu
Date: Fri, 23 Jun 2000 10:36:55 -0500 (CDT)
Subject: M&M 2000; Late-breaking poster session

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The deadline for contributions to the "LATE-BREAKING POSTER SESSION" at
MICROSCOPY AND MICROANALYSIS 2000 is very close at hand.

This poster session will be composed of presentations of newly acquired data or
analyses which were unavailable for submission by the February 15 deadline. A
short, half page abstract describing the studies is required. The abstract
should include: Title, Authors, Authors affiliation, and a Brief Description of
the studies. The description should include the Aim of the studies, a short
characterization of the Methods, and a brief account of the Results and their
Importance. The abstracts will obviously not be published in the proceedings,
since it is already being printed, but will be available at the meeting. The
posters will be advertized in the daily meeting newsletters to alert attendees
to their presence.

Abstracts should be e-mailed or faxed to the program chair, Stuart McKernan, at
stuartm-at-tc.umn.edu (email) or 612-625-5368 (fax). Abstracts must be received by
June 23, 2000. Abstracts will be reviewed by members of the program committee. A
limited number of poster boards are available and preference will be given to
early submissions. Abstract authors will be notified of acceptance of their
abstracts no later than July 1 (earlier for early submissions).

It is not too early to register for the meeting, the pre-meeting congress, or
the short courses (or any combination of these events). The early registration
deadline is also fast approaching, and can be done using the online registration
site at: http://www.peregrine.net/mm2000/

Looking forward to a great meeting in Philadelphia,



__________________
Stuart McKernan stuartm-at-tc.umn.edu
Director Office:(612) 626-7594
IT Characterization Facility, University of Minnesota Desk:(612) 624-6009
100 Union Street S. E., Minneapolis, MN 55455 NEW-} Fax:(612) 625-5368



From daemon Fri Jun 23 18:27:57 2000



From: Belluso elena :      belluso-at-dsmp.unito.it
Date: Fri, 23 Jun 2000 18:13:02 +0200 (MDT)
Subject: Need mineralogical samples for TEM studies

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello everyone,
I'm studying microstructures and intergrowths of fibrous minerals by TEM.
I'm looking for a sample of amosite and a sample of antophillite fibres in a
rock matrix in order to continue this study. I'm gratefully if someone will
send my these samples and will be interested to collaborate.

Thank you everyone,
Elena Belluso



----------------------------------------------------
Elena BELLUSO
Dipartimento di Scienze Mineralogiche e Petrologiche
Via Valperga Caluso, 35
I-10125 TORINO - ITALIA
tel:(39) 011.670.71.35 - fax: (39) 011.670.71.28
e-mail: belluso-at-dsmp.unito.it
http://www.dsmp.unito.it
----------------------------------------------------



From daemon Fri Jun 23 18:27:58 2000



From: Matthew Lynn :      mlynn-at-miami.edu
Date: Fri, 23 Jun 2000 12:26:59 -0400
Subject: RE: Strontium-based seashells?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Frank,

Yes...very interesting result! My guess is that you have happened upon a very
rare group of microplankton which make a celestite test (SrSO4). If I remember
my micropaleo, look for a group called Acantharia (?) Try Haq & Boersma's
"Marine Micropaleontology" for a start. The reason you have not come across
them in 20-odd years is that the celestite tests are very unstable...I saw a
few beautiful examples in filtered water from the Arabian Sea, never in
sediment samples. So not much use for paleoclimatology, but an interesting
diversion. If you can post some pics to a website, I'd like to take a look at
them.

Matt

Matthew J. Lynn, Ph.D.
Center for Advanced Microscopy
University of Miami
(305)284-4736
mlynn-at-miami.edu


On Friday, June 23, 2000 6:59 AM, Frank Thomas [SMTP:thomasf-at-AGC.BIO.NS.CA]
wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Here's an interesting problem. The other day I was examining some plankton
} trap samples in our ESEM, and found a few bits of what I took to be pieces
} of foraminiferal shell - they were slightly curved, crystalline-looking
} shards with closely spaced fine openings (the foramen, for which forams are
} named) and a few large holes (possible spine attachment localities). I ran
} EDS on them to make sure that is indeed, what they were. Planktic foram
} shells should be fairly pure calcium carbonate. What I got instead were
} spectra each showing a fairly large strontium peak, a smaller sulfur peak
} and a little oxygen. There were also very high aluminum peaks on each one,
} but since the sample was simply air dried on an aluminum stub, I assume
} that was the source of the Al.
} In 20-some years of micropaleontology I've never heard of any marine
} organism that uses strontium to build its shell. Sr is just below Ca in the
} periodic table, so I suppose it shares some of the same reactability
} characteristics (but I'm no chemist), so perhaps it could substitute, if no
} Ca was available (which would be darn funny in a surface marine sample).
} Let me add that all the other peaks were in their proper places, and the
} instrument had been calibrated quite recently, and I can think of no source
} of strontium contamination which could have gotten in there. We're planning
} on sending some of these bits out for analysis by some other means just to
} make sure, but for the time being, it's quite a mystery.
} Any ideas from the marine biologists? The chemists? The EDS gurus?
} Psychics?
}
}
} F.C. Thomas
} MicroAnalysis Facility
} Geological Survey of Canada (Atlantic)
} Bedford Institute of Oceanography
} Dartmouth, Nova Scotia
} Canada B2Y 4A2



From daemon Fri Jun 23 18:27:58 2000



From: Anthony Garratt-Reed :      tonygr-at-mit.edu
Date: Fri, 23 Jun 2000 11:57:45 -0400
Subject: Re: Hole in X-ray spectra

Contents Retrieved from Microscopy Listserver Archives
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Oldrich,

I think you have massive icing of your detector crystal, or equally massive
contamination of your window. I have modelled your Al spectrum (using
DTSA) and can get quite good agreement with your spectrum by putting in 300
microns of ice in the model. The low energy rise is probably caused simply
by the tail of the electronic noise which is amplified so much by the scale
at which you display your spectrum. Because of the way x-ray absorption
works, I can't really tell the difference between absorption in ice and
absorption in oil. Anyway, fixing an icing problem is far easier than
fixing an oil problem - you simply have to warm up the detector while
pumping on it. This will need an adaptor to fit on the pumping port of
your detector - your service engineer should have, or be able to get hold
of, one of these. So I would try warming the detector and seeing of it
solved the problem.

I wouldn't stake my professional reputation on this diagnosis, but I am
certain that if the problem is not contamination, then it is a geometrical
problem - the collimator may have moved, or some other mechanical
derangement taken place.

Good luck,

Tony Garratt-Reed.


At 11:11 AM 06/23/2000 +0200, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


** Anthony J. Garratt-Reed
** MIT Room # 13-1027
** 77 Massachusetts Avenue
** Cambridge, MA 02139-4307
** USA
**
** Phone: (+) 1-617-253-4622
** Fax: (+) 1-617-258-6479
**




From daemon Fri Jun 23 18:27:59 2000



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Fri, 23 Jun 2000 09:48:06 -0700
Subject: Re: undergrad SEM use

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Ken,
I have always run undergraduate courses on the SEM/EDX and we currently run
a course that puts groups of second year engineering students doing
independent research projects. They research the composition of many common
household and commercial appliances, taking them apart to see what metals,
plastics and ceramics are used for the various applications such as small
electric motors, heating elements and temperature control. The students are
very appreciative of being allowed to sit down and use the instrument with
minimal supervision and I find they are very careful with it.
I run courses for Anthropology and Electrical Engineering and all
disciplines of Engineering, Physics and Chemistry use the instrument for
both undergraduate and graduate research. I would suggest you get an EDX and
a low vacuum ("environmental") SEM for full versatility in both physical and
biological EM.
All of my EM instruments (two SEMs with EDX, one 200 kV TEM and SEM with
EBSP) are Hitachi, as I have found them the most robust. They run for years
of student use and nothing ever seems to go wrong with them. I had an ETEC
before my first Hitachi SEM and I liked it too, but they have been out of
business for a long time.
We also have a fourth year course that covers XRD, SEM, EDX and TEM/EDX. The
professor in charge has developed the lab course to touch all of the
important principles and demonstrate them in the lab. We cover changing kV,
EDX excitation volume, EDX matrix corrections, TEM use and TEM/EDX issues.
Please contact me if I can give you any other information.
At 03:05 PM 6/22/00 -0700, you wrote:
}
} Greetings:
} I am a new subscriber to this list and am looking for input/advice
} regarding the use of SEM's in undergraduate research. I teach at a liberal
} arts university with only undergraduate programs in the natural sciences.
} A geology colleague and myself (I'm a biologist) are exploring possible
} funding sources which would allow us to purchase an SEM that would be used
} by biology and geology undergrads in their independent and senior thesis
} projects. (We currently have a non-functioning ETEC that we would like to
} replace -- we've been advised that further repair and head-banging would
} not be cost effective). If anyone is currently engaged in SEM-based
} research with undergrads, I would like to get your feedback on your
} experiences (both the pluses and minuses). Although we don't have a TEM,
} input from those using TEM with undergrads would also be helpful.
}
} Specific Questions:
}
} 1) What types of projects are your students pursuing?
}
} 2) Are you conducting interdisciplinary projects? e.g., micropaleontology
} or environmental applications.
}
} 3) Are there particular models of SEMs which would be more user friendly
} since we would be training undergrads and we would also be the primary
} trainers and technicians?
}
} 4) Have you developed an undergrad EM course?
}
} Thanks,
} Ken

Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Fri Jun 23 18:28:00 2000



From: Robert Santoianni :      Robert_Santoianni-at-emory.org
Date: Fri, 23 Jun 2000 13:00:49 -0500
Subject: Re:TEM Darkroom/AGFA Papers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Date sent: Fri, 23 Jun 2000 11:57:42 -0400 (EDT)
} From: Donald Lovett {lovett-at-TCNJ.EDU}
To: Chris Jeffree {cjeffree-at-srv0.bio.ed.ac.uk}


When AGFA (Bayer) pulled the plug on the graded papers, we switched to
Kodak Polycontrast RCIII. So I pulled the plug on their activator and
fixer and now use Kodak Polymax developer 1:4 dilution and Rapid Fixer
in my DD3700. Still use my Durst S45EM with point light source and
Kodak Polycontrast Filter Kit.
Bob Santoianni
Emory University Hospital
Atlanta, GA



From daemon Fri Jun 23 18:28:01 2000



From: Caroline Schooley :      schooley-at-mcn.org
Date: Fri, 23 Jun 2000 10:14:58 -0700
Subject: Re: Strontium-based seashells?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} Here's an interesting problem. The other day I was examining some
} plankton
} trap samples in our ESEM, and found a few bits of what I took to be pieces
} of foraminiferal shell - they were slightly curved, crystalline-looking
} shards with closely spaced fine openings (the foramen, for which forams are
} named) and a few large holes (possible spine attachment localities). I ran
} EDS on them to make sure that is indeed, what they were. Planktic foram
} shells should be fairly pure calcium carbonate. What I got instead were
} spectra each showing a fairly large strontium peak, a smaller sulfur peak
} and a little oxygen. There were also very high aluminum peaks on each one,
} but since the sample was simply air dried on an aluminum stub, I assume
} that was the source of the Al.
} In 20-some years of micropaleontology I've never heard of any marine
} organism that uses strontium to build its shell. Sr is just below Ca in the
} periodic table, so I suppose it shares some of the same reactability
} characteristics (but I'm no chemist), so perhaps it could substitute, if no
} Ca was available (which would be darn funny in a surface marine sample).
} Let me add that all the other peaks were in their proper places,
} and the
} instrument had been calibrated quite recently, and I can think of no source
} of strontium contamination which could have gotten in there. We're planning
} on sending some of these bits out for analysis by some other means just to
} make sure, but for the time being, it's quite a mystery.
} Any ideas from the marine biologists? The chemists? The EDS gurus?
} Psychics?

Frank -

You've just rediscovered the strontianate Radiolaria. They DO selectively
concentrate strontium. I almost selected them as my thesis topic 'way
back in the dark ages; I dropped the idea when I realised that there was no
way to culture them in the lab. I don't think there is, yet.
}
Caroline

Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html




From daemon Fri Jun 23 18:28:02 2000



From: Doug Cromey :      doug-cromey-at-ns.arizona.edu
Date: Fri, 23 Jun 2000 10:36:41 -0700
Subject: Re: Stainning neurosecretory cells

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


This may be a bit of a shameless plug for a paper I contributed to, but
here's the reference:

Payne CM, Cromey DW (1987) Ultrastructural similarities between Chlorohydra
viridissima and human neurosecretory granules: A cytochemical study using
the uranaffin reaction. Cytobios 50:191-203.

Back when I was doing clinical TEM, we used the Uranaffin Method as the
"gold standard" for diagnostic identification of neurosecretory granules in
tumors. It was more reliable than immunomarkers and its fairly simple to
do. The reference section from this particular article should include Dr.
Payne's original publication on the technique. The most important thing is
to follow the protocol, failure to use the specified buffers usually causes
problems.

Yours,
Doug Cromey

At 09:46 AM 6/23/2000 -0400, you wrote:
} "A.P. Alves de Matos" wrote:
} } I just found some cells with granules resembling neurosecretory granules in
} } invertebrate tissues, and I need to confirm their nature.
} }
} } Can anyone recomend a straightforward and reliable method to identify
} } neurosecretory cells (at the light or/and electron microscopic levels)?
}
} Chrome-alum hematoxylin or Falhmi's aldehyde fuchsin work well with mammalian
} tissues. Check almost any edition of Humason's "Animal Tissue Techniques" for
} specifics. Modifications for invert tissues are out there but I don't have
} references handy.

....................................................................
: Douglas W. Cromey, M.S. Dept. of Cell Biology & Anatomy :
: Research Specialist, Principal University of Arizona :
: (office: AHSC 4212A) P.O. Box 245044 :
: (voice: 520-626-2824) Tucson, AZ 85724-5044 USA :
: (FAX: 520-626-2097) (email: doug-cromey-at-ns.arizona.edu):
:...................................................................:
http://www.pharmacy.arizona.edu/exp_path.html
Home of: "Microscopy and Imaging Resources on the WWW"



From daemon Fri Jun 23 18:28:02 2000



From: Matthew Lynn :      mlynn-at-miami.edu
Date: Fri, 23 Jun 2000 13:37:46 -0400
Subject: Re: Strontium-based seashells?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Sorry if this is double-posted....server troubles!

Frank,

Yes...very interesting result! My guess is that you have happened upon a very
rare group of microplankton which make a celestite test (SrSO4). If I remember
my micropaleo, look for a group called Acantharia (?) Try Haq & Boersma's
"Marine Micropaleontology" for a start. The reason you have not come across
them in 20-odd years is that the celestite tests are very unstable...I saw a
few beautiful examples in filtered water from the Arabian Sea, never in
sediment samples. So not much use for paleoclimatology, but an interesting
diversion. If you can post some pics to a website, I'd like to take a look at
them.

Matt


On Friday, June 23, 2000 6:59 AM, Frank Thomas [SMTP:thomasf-at-AGC.BIO.NS.CA]
wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Here's an interesting problem. The other day I was examining some plankton
} trap samples in our ESEM, and found a few bits of what I took to be pieces
} of foraminiferal shell - they were slightly curved, crystalline-looking
} shards with closely spaced fine openings (the foramen, for which forams are
} named) and a few large holes (possible spine attachment localities). I ran
} EDS on them to make sure that is indeed, what they were. Planktic foram
} shells should be fairly pure calcium carbonate. What I got instead were
} spectra each showing a fairly large strontium peak, a smaller sulfur peak
} and a little oxygen. There were also very high aluminum peaks on each one,
} but since the sample was simply air dried on an aluminum stub, I assume
} that was the source of the Al.
} In 20-some years of micropaleontology I've never heard of any marine
} organism that uses strontium to build its shell. Sr is just below Ca in the
} periodic table, so I suppose it shares some of the same reactability
} characteristics (but I'm no chemist), so perhaps it could substitute, if no
} Ca was available (which would be darn funny in a surface marine sample).
} Let me add that all the other peaks were in their proper places, and the
} instrument had been calibrated quite recently, and I can think of no source
} of strontium contamination which could have gotten in there. We're planning
} on sending some of these bits out for analysis by some other means just to
} make sure, but for the time being, it's quite a mystery.
} Any ideas from the marine biologists? The chemists? The EDS gurus?
} Psychics?
}

Matthew J. Lynn, Ph.D.
Center for Advanced Microscopy
University of Miami
(305)284-4736
mlynn-at-miami.edu



From daemon Fri Jun 23 18:28:02 2000



From: Matt Ervin :      mervin-at-ARL.mil
Date: Fri, 23 Jun 2000 13:38:27 -0400
Subject: A hole in the EDX spectrum II

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Oldrich-
I am sorry, but I have forgotten all of the details of your problem.
Have you thermally cycled your detector? letting the detector be a room
temperature for at least 24hrs can remove ice build-up in the detector. It
can also be useful to purge the dewar with nitrogen at that time to remove
any ice that may be in the dewar thus reducing cooling efficiency. I would
also suggest cleaning your detector window (I don't know what kind you
have.) Be very sure that you are using the proper technique and chemicals
for cleaning the window as the polymer windows are extremely easy to damage
(i.e., don't blow a dust particle off of it, see the current thread on
particulate damage to EDX windows.) In my case, as my window gets coated
with pump oil from the vacuum, etc. the Cu L line reduces in intensity
relative to the K line. For my super thin window the ratio should be L:K
} =2:1. If I put a thin graphite sheet between the detector and the sample,
I get spectra very similar to the ones you have posted with the low energy
x-rays blocked out (you'll still have the electronic noise peak at the
lowest energy) and then a normal looking higher energy range. Perhaps some
of this will help. I'll be on vacation the next couple of weeks, but give
me a call after 7 July if you have any questions I can help with.
Matthew Ervin
MErvin-at-ARL.mil
301-394-0017




Oldrich Benada {benada-at-biomed.cas.cz} on 06/23/2000 05:11:15 AM





To: Microscopy-at-sparc5.microscopy.com
cc:


Dear colleagues,
Many thanks for all responses to my question considering the hole in
the EDX spectrum.
We had a service engineer in our lab and he tried to solve the
problem but he was not successful.
I have prepared WEB page with the images of spectra, that were
recorded from following samples:
Cu_Al sample (usually used for calibration)
Ti grid
AL grid
K standard

The url of that page is following:
http://www.biomed.cas.cz/~benada/EDX_page/index.htm

Please, if anyone would be so kind and could comment the spectra, we
will be very happy.
Best regards from Prague
Oldrich Benada


+-----------------------------------+
Oldrich Benada
Acad. Sci. CR
Institute of Microbiology
Laboratory of electron microscopy
Videnska 1083
CZ - 142 20 Prague 4 - Krc
Czech Republic
+------------------------------------+
Phone: +420-2-4752399
Fax: +420-2-4715743
WEB:http://www.biomed.cas.cz/mbu/lem113/lem.htm






From daemon Fri Jun 23 18:28:04 2000



From: sherwood-at-helix.mgh.harvard.edu (Peggy Sherwood)
Date: Fri, 23 Jun 2000 13:45:54 -0400
Subject: AGFA Paper alternatives

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


To all:
We have been using Kodabrome II RC paper F1-F4 for years with our Durst
Laborator 1200 and
Rapidoprint DD3700. As of the end of 1999, the paper is still available
and gives good results.

Peggy Sherwood
Photopathology
Wellman Labs of Photomedicine-W224
70 Blossom Street
Boston, MA 02114

617-726-6983 (Lab)
617-724-4839 (Voice)
617-726-3192 (Fax)




From daemon Fri Jun 23 18:28:06 2000



From: Brian McIntyre :      mcintyre-at-optics.rochester.edu
Date: Fri, 23 Jun 2000 14:14:24 -0400
Subject: coin question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all-

Does anyone know how a really good counterfeiter would make a change in the
date field of a rare coin look as though it weren't altered? I've looked
for markings associated with moving the metal around, a joint between the
numbers and the base coin, and differing metallurgy, with no "smoking gun"....

Any thoughts??

Brian
----------------------------------------------
Brian McIntyre
Electron Microscopy Lab- River Campus
Univ of Rochester
Rochester, NY 14627
716-275-3058/4875



From daemon Fri Jun 23 18:28:07 2000



From: Matthew Lynn :      mlynn-at-miami.edu
Date: Fri, 23 Jun 2000 14:06:44 -0400
Subject: Re: Strontium-based seashells?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Sorry if this is double-posted....server troubles!

Frank,

Yes...very interesting result! My guess is that you have happened upon a very
rare group of microplankton which make a celestite test (SrSO4). If I remember
my micropaleo, look for a group called Acantharia (?) Try Haq & Boersma's
"Marine Micropaleontology" for a start. The reason you have not come across
them in 20-odd years is that the celestite tests are very unstable...I saw a
few beautiful examples in filtered water from the Arabian Sea, never in
sediment samples. So not much use for paleoclimatology, but an interesting
diversion. If you can post some pics to a website, I'd like to take a look at
them.

Matt


On Friday, June 23, 2000 6:59 AM, Frank Thomas [SMTP:thomasf-at-AGC.BIO.NS.CA]
wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Here's an interesting problem. The other day I was examining some plankton
} trap samples in our ESEM, and found a few bits of what I took to be pieces
} of foraminiferal shell - they were slightly curved, crystalline-looking
} shards with closely spaced fine openings (the foramen, for which forams are
} named) and a few large holes (possible spine attachment localities). I ran
} EDS on them to make sure that is indeed, what they were. Planktic foram
} shells should be fairly pure calcium carbonate. What I got instead were
} spectra each showing a fairly large strontium peak, a smaller sulfur peak
} and a little oxygen. There were also very high aluminum peaks on each one,
} but since the sample was simply air dried on an aluminum stub, I assume
} that was the source of the Al.
} In 20-some years of micropaleontology I've never heard of any marine
} organism that uses strontium to build its shell. Sr is just below Ca in the
} periodic table, so I suppose it shares some of the same reactability
} characteristics (but I'm no chemist), so perhaps it could substitute, if no
} Ca was available (which would be darn funny in a surface marine sample).
} Let me add that all the other peaks were in their proper places, and the
} instrument had been calibrated quite recently, and I can think of no source
} of strontium contamination which could have gotten in there. We're planning
} on sending some of these bits out for analysis by some other means just to
} make sure, but for the time being, it's quite a mystery.
} Any ideas from the marine biologists? The chemists? The EDS gurus?
} Psychics?
}

Matthew J. Lynn, Ph.D.
Center for Advanced Microscopy
University of Miami
(305)284-4736
mlynn-at-miami.edu



From daemon Sat Jun 24 12:32:27 2000



From: Earl Weltmer :      earlw-at-pacbell.net
Date: Fri, 23 Jun 2000 18:24:16 -0700
Subject: Re: coin question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Brian,

I've thought about this before.

A really good counterfeiter could do this at the molecular level by using and FIB
or equivalent.

I haven't really explored exactly how this could be done but it has crossed my
mind.


regards,

Earl Weltmer

Brian McIntyre wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hi all-
}
} Does anyone know how a really good counterfeiter would make a change in the
} date field of a rare coin look as though it weren't altered? I've looked
} for markings associated with moving the metal around, a joint between the
} numbers and the base coin, and differing metallurgy, with no "smoking gun"....
}
} Any thoughts??
}
} Brian
} ----------------------------------------------
} Brian McIntyre
} Electron Microscopy Lab- River Campus
} Univ of Rochester
} Rochester, NY 14627
} 716-275-3058/4875



From daemon Sat Jun 24 13:23:14 2000



From: Rajesh Patel :      rpatel-at-UMDNJ.EDU
Date: Sat, 24 Jun 2000 12:39:21 -0500
Subject: stain

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


There was a recent posting about UA precipitating during staining.

Well wouldn't you know I too have experinecing similar problem. It
took me a long time to figure out but I narrowed it down to our
rinsing water that is used to rinse the grid in after it is stained.

After chaging the filters on our water purification system the problem was
gone.

Rajesh Patel
Robert Wood Johnson Medical School
Dept. of Pathology
Electron Microscopy Lab
675 Hoes Lane
Piscataway, NJ 08854

(732)235-4648
rpatel-at-umdnj.edu




From daemon Sat Jun 24 15:29:00 2000



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Sat, 24 Jun 2000 14:14:10 -0600
Subject: coin question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Brian,

how about looking at sub-surface damage (using some channeling techniques or
similar)? I could imagine, that the damage from stamping the coin is
different from Ion bombarding the coin.

Or, look for implanted atoms. The FIB process uses metal atoms to bombard
the sample. Some of them get implanted into the material. So you might find
higher concentrations where the sample was altered.

Finally, how about a surface layer like an oxide or patina. There may be
differences between the very old layer and the new layer.

Michael

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Brian McIntyre [mailto:mcintyre-at-optics.rochester.edu]
Sent: Friday, June 23, 2000 12:14 PM
To: Microscopy-at-sparc5.microscopy.com


Hi all-

Does anyone know how a really good counterfeiter would make a change in the
date field of a rare coin look as though it weren't altered? I've looked
for markings associated with moving the metal around, a joint between the
numbers and the base coin, and differing metallurgy, with no "smoking
gun"....

Any thoughts??

Brian
----------------------------------------------
Brian McIntyre
Electron Microscopy Lab- River Campus
Univ of Rochester
Rochester, NY 14627
716-275-3058/4875



From daemon Mon Jun 26 20:51:51 2000



From: Jo Verbeeck :      joverbee-at-ruca.ua.ac.be
Date: Mon, 26 Jun 2000 09:29:37 +0200 (METDST)
Subject: Power stability in microscopy and EELS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello all,

Over time I saw some questions regarding the installation of a UPS or some
power conditioner to improve the stability of all sorts of microscopy
equipment.
I understand that quite a few instruments are sensitive to the quality of
the line power they get.
As I was working on EMC (electro magnetic compatibility) in a former life,
this seems strange to me.
In Europe we have very strict EC-rules that guarantee that equipment meets
its specifications even under severe conditions (including HF noise on the
power, fluctuations in the power, high EM fields, temperature variations
etc)

I agree that microscopes are far more sensitive devices than, say,
videogames but does anyone know of the EMC rules for microscopes and
related equipment?
It seems strange that some people are using UPS's while especially power
fluctuations can be easily overcome with a good design of the
electronics...

Regards,

Jo

*************************************************************
* Jo Verbeeck *
* University of Antwerp *
* Dept. EMAT (Electron Microscopy for Materials Research) *
* e-mail: joverbee-at-ruca.ua.ac.be *
* tel: +32(0)3 218 02 49 *
* fax: +32(0)3 218 02 57 *
*************************************************************



From daemon Mon Jun 26 20:51:52 2000



From: oshel-at-terracom.net (Philip Oshel)
Date: Mon, 26 Jun 2000 06:01:20 -0500
Subject: protists & SrSO4

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I've got my references now:
The protists with SrSO4 tests are the class *Acantherea*, members of the
phylum Sarcodina (amoebae), subphylum Actinopoda (used to be known as the
Radiolaria).

By my somewhat outdated reference ("A Synoptic Classification of Living
Organisms", R.S.K. Barnes, ed.). The names have likely been changed, and
the Actinopoda broken up to protect the careers of systematists.

Forget I ever mentioned Actinaria or Acanthella. The long-term storage in
my wetware is getting flakey (I misremembered).

Phil

}}}}}}}}}}}}}}}}}{{{{{{{{{{{{{{{{{
Philip Oshel
Supervisor, AMFSC and BBPIC
Dept. of Animal Health and Biomedical Sciences
University of Wisconsin
1656 Linden Drive
Madison, WI 53706-1581
Voice: (608) 263-4162
peoshel-at-facstaff.wisc.edu
fax: (608) 262-7420 (dept. fax)




From daemon Mon Jun 26 20:51:53 2000



From: HARRISm-at-esm-semi.co.uk
Date: Mon, 26 Jun 2000 12:04 +0000 (GMT)
Subject: Electron Microscope Maintenance

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hi ,


Could anyone recommend an independent U.K based company
specialising in the repair and maintenance of electron microscopes ?

Without mentioning names I feel that the service we receive re our SEM
maintenance contract is particularly poor and am beginning to wonder
is there any alternative to complaint without improvement and excuse
after excuse .

I have noticed that little repair is carried out at component level
during service engineer visits but am not aware of how ' specific '
most parts are to a particular make of instrument . If parts always
have to be ordered through the instrument supplier there would be no
advantage in independent maintenance .
Another alternative perhaps would be in-house maintenance but do
courses exist to train the novice type like myself to a reasonably
competent level or is this not advised ?

Keen to hear comments ...

M.Harris Email harrism-at-esm-semi.co.uk
ESM LTD ,
South Wales , U.K





From daemon Mon Jun 26 20:51:54 2000



From: Rudi Lurz :      Lurz-at-molgen.mpg.de
Date: Mon, 26 Jun 2000 13:02:29 +0200
Subject: RE: AGFA Paper

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} Date: Mon, 26 Jun 2000 09:56:43 +0200
} To: Microscopy-at-sparc5.microscopy.com
} From: Rudi Lurz {Lurz-at-molgen.mpg.de}
} Subject: Re: ***TEM Darkroom Users*** AGFA Paper has become extinct !
}
} To all:
} The identical technology (developer in emulsion + activator) as with
} Rapidoprint papers is used with Agfa Brovira-Speed papers. I have replaced
} years ago the Rapitone paper by Brovira-Speed 310 RC because I prefered
} more the blue-black of Brovira-Speed to Rapitone which showed more brown
} tendency. Brovira-Speed is available from soft to extra hard and should be
} still produced (hopefully) as I was told from my dealer this morning.
} This is for Germany - I do not know the situation in the US.
} Agfa seems to reduce drastically the B/W programme. The Agfa Scientia
} 23D56 films are no longer produced too and we have to switch to Kodak SO-163.
} Best regards
} Rudi Lurz


_________________________________
Rudi Lurz Phone: X - 30-8413-1271
MPI fŸr Molekulare Genetik Fax: X - 30-8413-1385
Ihnestrasse 73
D-14195 Berlin E-mail to: Lurz-at-molgen.mpg.de





From daemon Mon Jun 26 20:52:01 2000



From: Philip Oshel :      peoshel-at-facstaff.wisc.edu
Date: Mon, 26 Jun 2000 08:15:18 -0500
Subject: Re: undergrad SEM use

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Teaching microscopy at both the undergrad and grad student levels
would be an excellent symposium or discussion group at the Long Beach
meeting. Especially since the life of EM facilities depends on having
users, and getting students interested in microscopy is one of the
best ways to create users.

Phil

} } Greetings:
} } I am a new subscriber to this list and am looking for input/advice
} } regarding the use of SEM's in undergraduate research. I teach at a liberal
} } arts university with only undergraduate programs in the natural sciences.
} } A geology colleague and myself (I'm a biologist) are exploring possible
} } funding sources which would allow us to purchase an SEM that would be used
} } by biology and geology undergrads in their independent and senior thesis
} } projects. (We currently have a non-functioning ETEC that we would like to
} } replace -- we've been advised that further repair and head-banging would
} } not be cost effective). If anyone is currently engaged in SEM-based
} } research with undergrads, I would like to get your feedback on your
} } experiences (both the pluses and minuses). Although we don't have a TEM,
} } input from those using TEM with undergrads would also be helpful.
} }
} } Specific Questions:
} }
} } 1) What types of projects are your students pursuing?
} }
} } 2) Are you conducting interdisciplinary projects? e.g., micropaleontology
} } or environmental applications.
} }
} } 3) Are there particular models of SEMs which would be more user friendly
} } since we would be training undergrads and we would also be the primary
} } trainers and technicians?
} }
} } 4) Have you developed an undergrad EM course?
} }
} } Thanks,
} } Ken
--
}}}}}}}}}}}}}}}}}{{{{{{{{{{{{{{{{{
Philip Oshel
Supervisor, AMFSC and BBPIC
Dept. of Animal Health and Biomedical Sciences
University of Wisconsin
1656 Linden Drive
Madison, WI 53706-1581
voice: (608) 263-4162
fax: (608) 262-7420 (dept. fax)



From daemon Mon Jun 26 20:52:08 2000



From: Brian McIntyre :      mcintyre-at-optics.rochester.edu
Date: Mon, 26 Jun 2000 11:22:39 -0400
Subject: coin question followup

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


hi...and thanks for the helpful insights thusfar.

i should clarify that the coin in question could not have been altered
after 1900 so modern techniques are obviously out of the question. the
issue becomes one of... if you wanted to alter a coin's date how would you
have done it 100 years ago, and how could it be uncovered using modern
analysis techniques.

thanks!
b-
----------------------------------------------
Brian McIntyre
Electron Microscopy Lab- River Campus
Univ of Rochester
Rochester, NY 14627
716-275-3058/4875



From daemon Mon Jun 26 20:52:08 2000



From: Allen R. Sampson :      ars-at-sem.com
Date: Mon, 26 Jun 2000 11:33:13 -0500
Subject: RE: coin question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Use of an x-ray microprobe should be rather definitive, I would think.
While there are exotic (and expensive) techniques that could be used to
remove a raised date and place a new one, it would be extremely difficult
to exactly match the elemental contents, particlarly those of the trace
elements. For example, gold, even when refined to 99.999% pure, contains
enough impurities for any batch to be matched to a particular mine that it
came from.

The only other method I could imagine is a close characterization of the
grain structures. I don't know if the stamping process would have much of
an affect on the surface grain, but I imagine any implantation method would
leave a much different structure as completely different conditions were
used to form the metal.



Allen R. Sampson, Owner
Advanced Research Systems, St. Charles, Illinois 60174
voice 630.513.7093 fax 630.513.7092

On Friday, June 23, 2000 1:14 PM, Brian McIntyre
[SMTP:mcintyre-at-optics.rochester.edu] wrote:

} Hi all-
}
} Does anyone know how a really good counterfeiter would make a change in
the
} date field of a rare coin look as though it weren't altered? I've looked
} for markings associated with moving the metal around, a joint between the
} numbers and the base coin, and differing metallurgy, with no "smoking
gun"....
}
} Any thoughts??
}
} Brian
} ----------------------------------------------
} Brian McIntyre
} Electron Microscopy Lab- River Campus
} Univ of Rochester
} Rochester, NY 14627
} 716-275-3058/4875
}
}



From daemon Mon Jun 26 20:52:11 2000



From: Mark West :      mwest-at-ifcsun1.ifisiol.unam.mx
Date: Mon, 26 Jun 2000 13:36:33 -0600 (Hora est‡ndar de MŽxico)
Subject: frogs' eggs and vesicles

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

A couple of weeks ago I wrote in witha problem trying to fix plant vesicle
preps for LR White inclusion and immunogold studies, without osmicating.
The pellets were disappearing during dehydration. I included an osmium
step (5, 30 or 60 minutes) and the pellets stopped disappearing, so I
guess there is so little protein that a formaldehyde/gluteraldehyde fix
doesn't manage to stabilize them. I'll have to check whether these
osmicated vesicles work in the immunogold reaction and check the effect
of a metaperiodate treatment to expose the proteins. Thanks for the advice
- another problem solved.

Someone brought us some frogs' eggs to fix. It looks like they have a hard
skin. The researcher is interested in extracting the nuclei (huge) which
also look like they're enclosed in a hard skin, and he wants to know
whether the extracted nuclei still retain some endoplasmic reticulum, so I
thought the best way would be to compare a whole egg with an extracted
nucleus. Any ideas/experience on how to fix this thing?

Thanks,

Mark






********************************************
Mark West,
Unidad de Microscopia Electronica,
(Electron Microscopy Unit)
Instituto de Fisiologia Celular,
Universidad Nacional Autonoma de Mexico,
04510 Mexico D.F.

tel (unidad/lab) *(525) 622 5610*
(casa/home) (525) 619 3020
Fax (525) 616 2282
********************************************



From daemon Mon Jun 26 20:52:15 2000



From: Heejun Jeong :      hjjeong -at-physics.purdue.edu
Date: Tue, 27 Oct 1998 14:47:09 -0500
Subject: question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,

I just want some opinions about the problem I got recently with our SEM.

We use this machine for electron beam lithography. This application is a
little more sensitive than imaging. The problem I have now is, when I
image at very low magnification, I can see the circular rim of the final
section of the column. But from a couple of weeks, it changed suddenly,
now the circular image is distorted like an oval shape and at second
saturation point, normally only one position of gun alignment gives the
maximam beam current, but I see there are clearly two distinct, well
separated positions. We think something is blocking in the middle of the
beam path, but want to know whether there is any other one who
experienced similar thing and know the reason.
I appreciate if anyone can give me his/her similar experince.

Heejun Jeong
hjjeong-at-physics.purdue.edu




From daemon Mon Jun 26 20:52:15 2000



From: Orion Analytical :      orionanalytical-at-worldnet.att.net
Date: Mon, 26 Jun 2000 15:47:14 -0400
Subject: Re: coin question followup

Contents Retrieved from Microscopy Listserver Archives
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Coins have been faked outright and updated using modern dies, and assembled
from two separate halves using solder. Coins whose value depends on date
have been "enriched" by removing the original date and soldering in its
place numbers of a more valuable date. This latter case might be discerned
using scanning electron microscopy to examine surface texture and
degradation and adjunct energy-dispersive analysis to detect the presence of
solder.

James Martin
Principal/Research Scientist
Orion Analytical, LLC
P.O. Box 550
Williamstown, MA 01267
www.orionanalytical.com

} i should clarify that the coin in question could not have been altered
} after 1900 so modern techniques are obviously out of the question. the
} issue becomes one of... if you wanted to alter a coin's date how would you
} have done it 100 years ago, and how could it be uncovered using modern
} analysis techniques.
}
} thanks!
} b-
} ----------------------------------------------
} Brian McIntyre
} Electron Microscopy Lab- River Campus
} Univ of Rochester
} Rochester, NY 14627
} 716-275-3058/4875
}



From daemon Mon Jun 26 20:52:16 2000



From: Caroline Schooley :      schooley-at-mcn.org
Date: Mon, 26 Jun 2000 13:03:39 -0700
Subject: Re: undergrad SEM use

Contents Retrieved from Microscopy Listserver Archives
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} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

You don't have to wait that long! Go to the Philadelphia meeting web page
search engine http://www.msa.microscopy.com/cgi-bin/M&M00Program.pl and
enter "teaching microscopy". Steve Barlow has organized an excellent
all-day session.

Caroline



Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html




From daemon Mon Jun 26 20:52:20 2000



From: Larry Stoter :      LPS-at-teknesis.demon.co.uk
Date: Mon, 26 Jun 2000 21:16:07 +0100
Subject: Re: Electron Microscope Maintenance

Contents Retrieved from Microscopy Listserver Archives
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} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Clearly, I have several vested interests here! As I am answering this
from home, I don't actually know as I write if you are specifically
concerned about a JEOL SEM, although I will check when I get to the
office tomorrow.

First, I would suggest you complain, with specifics, to the service
manager of the relevant company. If that is ineffective, direct your
complaint to the Salesperson covering your area - sales people,
perhaps, have a much stronger vested interest in happy customers. A
happy customer is possibly going to buy again in the future, an
unhappy customer makes their feelings known to others and can
influence future sales.

I hope you are not a JEOL user - if you are, please contact me
directly and I will attempt to resolve your problems.

If the SEM in question is an older instrument, independent service
companies will do a good job on all of the more routine problems. You
should also be able to resolve these yourself, in principle, with a
reasonable knowledge of vacuum technology and electronics.

I don't know where you might get training on SEM service - possibly Protrain?

The two main independent service companies that I am aware of
operating in the UK are ISS and EOS, both based in the Manchester
area but with engineers throughout most of the UK. Contact details
from sources on the web and Microscopy & Analysis. If you contect me
directly, I will forward you details.

Problems arise with newer instruments, especially PC-controlled ones.
Self-service and independent service will cover the basics but
without detailed training, circuit diagrams, software tools, it gets
very difficult to handle anything more than the basics.

Incidentally, this is one of the disadvantages for customers of
computer controlled EMs - it makes it easier for ALL manufacturers to
restrict service to approved service engineers. Not that this happens
just with scientific instruments - a similar issue exists with
cars/automobiles. I believe there is/has been a court case in the US
by independent auto-repair companies against the manufacturers to
force them to release service software.

Even on older instruments, major problems can be difficult to resolve
for anybody other than the manufacturer.

You will probably find that service via independents is cheaper than
via the manufacturers. Specialist parts will have to be purchased
from the manufacturer but even then, the overall cost will probably
be lower. However, I would add that I don't believe any EM
manufacturer is interested in providing anything other than
value-for-money service and, to a great extent, you do get what you
pay for - at least, you should! If you are paying less, overall, the
service you get is probably less - it may not be obvious, and you may
never see the difference but, in the long run, you will get what you
pay for.

You also need to keep in mind that the more comprehensive service
contracts provided by manufactuers contain a significant 'insurance'
element. Major components are expensive - with a comprehensive
service contract from the maufacturer, you will never know how
expensive but with service via an independent, you may find out!

Hope that is useful.

Best regards,
--
Larry Stoter
JEOL (UK) Ltd
Silver Court, Watchmead, Welwyn Garden City, AL7 1LT, United Kingdom
tel: +44-(0)1707-377117, fax: +44-(0)1707-373254, e-mail: larrys-at-jeoleuro.com



From daemon Mon Jun 26 20:52:20 2000



From: A. Greene :      ablue-at-io.com
Date: Monday, June 26, 2000 4:10 PM
Subject: question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,

It would very much help to know what sort of an SEM you are using and at
what kV you are operating. I suspect you may have had a minor vacuum
accident which caused one of your "spray apertures" to be blown out of
position by inrushing air. Pull the liner tube (if your instrument has one)
and reposition all the apertures. It might not be a bad idea to do a good
cleaning job while inside the column.

Good luck.

Alex Greene
SCIENTIFIC INSTRUMENTATION SERVICES, INC.
PMB-499, 1807 West Slaughter Lane, Number 200
Austin, Texas 78748-6200
Phone: 512/282-5507 Fax: 512/280-0702

QUALITY ELECTRON MICROSCOPE REPAIR
-----Original Message-----
} From: Heejun Jeong {" hjjeong"-at-physics.purdue.edu} {Heejun Jeong {"
hjjeong"-at-physics.purdue.edu} }
To: Microscopy-at-sparc5.microscopy.com {Microscopy-at-sparc5.microscopy.com}



From daemon Mon Jun 26 20:52:28 2000



From: Harry Turner :      gen28t-at-happypuppy.com
Date: Mon, 26 Jun 2000 19:20:16 -0500
Subject: Between us... #3C9F

Contents Retrieved from Microscopy Listserver Archives
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From daemon Tue Jun 27 08:53:03 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 26 Jun 2000 20:51:29 -0700
Subject: Re: Power stability in microscopy and EELS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


There are essentially two types of UPS systems. One acts in
a passive mode and switches to battery backup when power
fails. The other is an active mode which converts AC input
to DC and uses this to generate stable AC output.

Power conditioners are typically microprocessor-controlled units which
adjust taps on a transformer to maintain reasonably stable
output voltage. These can also include traps for spikes and
overvoltage conditions (Topaz for example).

The issue here is the stability of the basic potentials and currents
which are fed to or are delivered to the electron gun. In this
regard, I think that we are talking about very narrow margins of
input voltage variation and output voltages. I think that one will
have line sag no matter what--on occasion. Thus, the active
UPS units bypass these conditions and provide a constant,
stable AC supply to the SEM. Line conditioners are rated at
+/- 6% down to +/- 3%. Active UPS can do better.... but their
surge current capabilities are less than those of the conditioner units.

Consequently, there is not one, singularly perfect solution. But there are
options which the user should consider.

gg

At 12:29 AM 6/26/00, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Tue Jun 27 08:53:15 2000



From: Gordon Couger :      gcouger-at-couger.com
Date: Tue, 27 Jun 2000 04:34:29 -0500
Subject: Re: Power stability in microscopy and EELS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} From a practical point of view my experience with computer controlled UPS
systems is that cause more problems than they solve. They provide a
computer with good enough power in most cases. This power is not good
enough for equipment that is not designed for considerable voltage changes
and micro power losses.

Most of my experience comes from computer back up power supplies and a
project that was measuring sunlight. Since it was sunlight and we needed
fast response we did not have any low pass filter. When working on it in
the lab I used filtered DC since I was having problems with the 120 Hz
flicker of incandescent bulbs. I could see sags and glitches in the AC
power through the UPS systems even with the filters. It was good enough
for what I was doing but I could see a good deal difference in the
stability of the UPS and a battery.

I have worked with a few radio systems that use AC to charge a battery
bank and then power the radios from the batteries. These are far more
reliable than UPS systems.


Gordon
Gordon Couger gcouger-at-couger.com
Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00

} From: "Dr. Gary Gaugler" {gary-at-gaugler.com}
}
} There are essentially two types of UPS systems. One acts in
} a passive mode and switches to battery backup when power
} fails. The other is an active mode which converts AC input
} to DC and uses this to generate stable AC output.
}
} Power conditioners are typically microprocessor-controlled units which
} adjust taps on a transformer to maintain reasonably stable
} output voltage. These can also include traps for spikes and
} overvoltage conditions (Topaz for example).
}
} The issue here is the stability of the basic potentials and currents
} which are fed to or are delivered to the electron gun. In this
} regard, I think that we are talking about very narrow margins of
} input voltage variation and output voltages. I think that one will
} have line sag no matter what--on occasion. Thus, the active
} UPS units bypass these conditions and provide a constant,
} stable AC supply to the SEM. Line conditioners are rated at
} +/- 6% down to +/- 3%. Active UPS can do better.... but their
} surge current capabilities are less than those of the conditioner units.
}
} Consequently, there is not one, singularly perfect solution. But there
are
} options which the user should consider.
}
} gg
}

} }
} } Hello all,
} }
} } Over time I saw some questions regarding the installation of a UPS or
some
} } power conditioner to improve the stability of all sorts of microscopy
} } equipment.
} } I understand that quite a few instruments are sensitive to the quality
of
} } the line power they get.
} } As I was working on EMC (electro magnetic compatibility) in a former
life,
} } this seems strange to me.
} } In Europe we have very strict EC-rules that guarantee that equipment
meets
} } its specifications even under severe conditions (including HF noise on
the
} } power, fluctuations in the power, high EM fields, temperature
variations
} } etc)
} }
} } I agree that microscopes are far more sensitive devices than, say,
} } videogames but does anyone know of the EMC rules for microscopes and
} } related equipment?
} } It seems strange that some people are using UPS's while especially
power
} } fluctuations can be easily overcome with a good design of the
} } electronics...
} }
} } Regards,
} }
} } Jo

}








From daemon Tue Jun 27 08:53:17 2000



From: donald j marshall :      dmrelion-at-world.std.com
Date: Tue, 27 Jun 2000 08:16:12 -0400 (EDT)
Subject: glass window thicknesses

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am trying to locate a copy of a graph that shows the thicknesses of
(glass) windows that can support atmospheric pressure against a vacuum for
various radii of O ring support. Kaufman glass has one for high pressures
for several glass types but it doesn't cover the range of atmospheric
pressure and relatively small radii(1 to 3 cm). Pyrex, leaded glass, and
quartz are the three principal materials of interest. Appreciate any leads.

Don Marshall

Donald J. Marshall
Relion Industries
P.O. Box 12
Bedford, MA 01730
Ph: 781-275-4695
FAX: 781-271-0252
email dmrelion-at-world.std.com

Cathodoluminescence, mass spectroscopy, electron beam technology


"A weed is a flower out of place."

(Please note: Do not send email with attachments to this address. Instead, send it to donbarlen-at-aol.com. Thank you.)



From daemon Tue Jun 27 08:53:19 2000



From: Rahbari, Ramin :      RAMIN.RAHBARI-at-WL.com
Date: Tue, 27 Jun 2000 09:15:04 -0400
Subject: frogs' eggs and vesicles

Contents Retrieved from Microscopy Listserver Archives
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You may want to contact
David Gard, Ph.D., Biology Department, University of Utah, Salt Lake City,
UT.
He has done a great deal of work with frog eggs.

good luck

Ramin Rahbari
Pfizer Global Research & Development
Worldwide Preclinical Safety
2800 Plymouth Road
Ann Arbor, MI 48105
Voice (734) 622-3383
Fax (734) 622-5001
Ramin.Rahbari-at-WL.COM


-----Original Message-----
} From: Mark West [mailto:mwest-at-ifcsun1.ifisiol.unam.mx]
Sent: Monday, June 26, 2000 3:37 PM
To: Microscopy-at-sparc5.microscopy.com


Hi,

A couple of weeks ago I wrote in witha problem trying to fix plant vesicle
preps for LR White inclusion and immunogold studies, without osmicating.
The pellets were disappearing during dehydration. I included an osmium
step (5, 30 or 60 minutes) and the pellets stopped disappearing, so I
guess there is so little protein that a formaldehyde/gluteraldehyde fix
doesn't manage to stabilize them. I'll have to check whether these
osmicated vesicles work in the immunogold reaction and check the effect
of a metaperiodate treatment to expose the proteins. Thanks for the advice
- another problem solved.

Someone brought us some frogs' eggs to fix. It looks like they have a hard
skin. The researcher is interested in extracting the nuclei (huge) which
also look like they're enclosed in a hard skin, and he wants to know
whether the extracted nuclei still retain some endoplasmic reticulum, so I
thought the best way would be to compare a whole egg with an extracted
nucleus. Any ideas/experience on how to fix this thing?

Thanks,

Mark






********************************************
Mark West,
Unidad de Microscopia Electronica,
(Electron Microscopy Unit)
Instituto de Fisiologia Celular,
Universidad Nacional Autonoma de Mexico,
04510 Mexico D.F.

tel (unidad/lab) *(525) 622 5610*
(casa/home) (525) 619 3020
Fax (525) 616 2282
********************************************



From daemon Tue Jun 27 18:12:00 2000



From: James M. Ehrman :      jehrman-at-mta.ca
Date: Tue, 27 Jun 2000 10:59:41 -0300
Subject: EDS on SEM w/o specimen airlock

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all,

We're in the process of acquiring an EDS system for our JEOL 5600 and
I'm curious about the experience of others with EDS systems on scopes
without specimen airlocks (column vents to exchange specimens). Any
thoughts/recommendations? I'm particularly concerned with contamination,

window integrity and the like. Are there any advantages/disadvantages of

Be vs. thin window detectors here?

Thanks,

Jim Ehrman

--

James M. Ehrman
Digital Microscopy Facility
Mount Allison University
Sackville, NB E4L 1G7
CANADA

phone: 506-364-2519
fax: 506-364-2505
email: jehrman-at-mta.ca
www: http://www.mta.ca/~jehrman




From daemon Tue Jun 27 18:12:03 2000



From: Dee Breger :      micro-at-ldeo.columbia.edu
Date: Tue, 27 Jun 2000 10:20:31 -0400 (EDT)
Subject: LM geological stain question

Contents Retrieved from Microscopy Listserver Archives
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Dear colleagues,

I've received a query from a climatologist who wants to photograph fine
varves and/or laminations (~0.5 mm- 1 mm each layer) in carbonate (calcite)
rich silt (or marl) through a binocular light microscope. The samples are
flat, finely cut or polished sections that can be etched if necessary, but
he's really looking for a staining method that would enhance the difference
between the carbonate-rich layers and the clay-rich layers to make them
more usefully photogenic. Can anyone advise us on how to proceed to best
advantage?

Many thanks,
Dee




***************************************************************
Dee Breger
Mgr. SEM/EDX Facility
Lamont-Doherty Earth Observatory
61 Route 9W
Palisades, NY 10964 USA
T: 914/365-8640
F: 914/365-8155

http://www.ldeo.columbia.edu/micro
http://www.discovery.com/area/science/micro/micro1.html
http://www.lsc.org/antarctica/front.html
Journeys in Microspace (Columbia University Press, 1995)




From daemon Tue Jun 27 18:12:03 2000



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Tue, 27 Jun 2000 10:13:20 -0500
Subject: Re: EDS on SEM w/o specimen airlock

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have two different models of thin window detectors on two different
scopes. Our Hitachi 2460N does not have an airlock and is vented for every
sample change. Our JEOL 840A has an airlock, but does get the chamber
vented fairly often. We have only had one pin-hole develop in the detector
on our 840 in the 15-20 instrument-years that we have had the detectors.

Therefore, I don't think you would be gaining much, if anything, by
switching to a Be window. And you would be giving up that wonderful light
element capability. I would be hard pressed not to be able to detect C and
O. I would go for the thin window and be reasonably careful.

Warren S.

At 10:59 AM 6/27/2000 -0300, you wrote:

} Hi all,
}
} We're in the process of acquiring an EDS system for our JEOL 5600 and
} I'm curious about the experience of others with EDS systems on scopes
} without specimen airlocks (column vents to exchange specimens). Any
} thoughts/recommendations? I'm particularly concerned with contamination,
}
} window integrity and the like. Are there any advantages/disadvantages of
}
} Be vs. thin window detectors here?
}
} Thanks,
}
} Jim Ehrman
}
} --
}
} James M. Ehrman
} Digital Microscopy Facility
} Mount Allison University
} Sackville, NB E4L 1G7
} CANADA
}
} phone: 506-364-2519
} fax: 506-364-2505
} email: jehrman-at-mta.ca
} www: http://www.mta.ca/~jehrman
}



From daemon Tue Jun 27 18:12:03 2000



From: Vickie Frohlich :      frohlich-at-uthscsa.edu
Date: Tue, 27 Jun 2000 10:22:54 -0500
Subject: Re: Electron Microscope Maintenance

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} Could anyone recommend an independent U.K based company
} specialising in the repair and maintenance of electron microscopes ?
}
} Keen to hear comments ...


Information forwarded to list for Tributary Business Consulting:

I am an expense reduction professional. I have experience with equipment
maintenance contracts, and with Original Equipment Manufacture and
third-party equipment maintenance providers throughout the world. Please
contact me directly if you are interested.

Charles R. Frohlich CPA
President
Tributary Business Consulting
email: cfrohlich-at-satx.rr.com
phone: (210) 695-1364
fax: (210) 695-2141



From daemon Tue Jun 27 18:12:04 2000



From: George Lawton :      George.Lawton-at-email.swmed.edu
Date: Tue, 27 Jun 2000 10:03:18 -0500
Subject: SEM: Mounting Fly Heads

Contents Retrieved from Microscopy Listserver Archives
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An investigator just brought me 6 samples - heads of flies. He was instructed to bring the entire body but he cuts the heads off when he does TEM. The investigator knows nothing about SEM. I have done alot of scanning of fly eyes but the body is attached.
My question is: how do I mount the heads on the stubs without the graphite or silver paint causing capillary action over the entire head? We will scan on a JEOL 840A after Au/Pd coating.

George Lawton
Chief Electron Microscopist
Molecular and Cellular Imaging Facility
UT Southwestern Medical Center at Dallas
5323 Harry Hines Blvd.
Dallas, Tx 75390-9039
Phone: 214-648-7291
Fax 214-648-6408
eMail: George.Lawton-at-email.swmed.edu



From daemon Tue Jun 27 18:12:07 2000



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Tue, 27 Jun 2000 11:14:41 -0500
Subject: SEM: Mounting Fly Heads

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


George,

I would use one of the double-sided carbon sticky tabs available from
several vendors. They are good for small samples that would be buried in
liquids. If they don't provide enough contact area, you could very
carefully dab a conductive paint around the base of the mounted heads with a
pointy stick.

Cheers,
Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/




-----Original Message-----
} From: George Lawton [mailto:George.Lawton-at-email.swmed.edu]
Sent: Tuesday, June 27, 2000 10:03 AM
To: microscopy-at-sparc5.microscopy.com


An investigator just brought me 6 samples - heads of flies. He was
instructed to bring the entire body but he cuts the heads off when he does
TEM. The investigator knows nothing about SEM. I have done alot of
scanning of fly eyes but the body is attached.
My question is: how do I mount the heads on the stubs without the graphite
or silver paint causing capillary action over the entire head? We will scan
on a JEOL 840A after Au/Pd coating.

George Lawton
Chief Electron Microscopist
Molecular and Cellular Imaging Facility
UT Southwestern Medical Center at Dallas
5323 Harry Hines Blvd.
Dallas, Tx 75390-9039
Phone: 214-648-7291
Fax 214-648-6408
eMail: George.Lawton-at-email.swmed.edu



From daemon Tue Jun 27 18:12:09 2000



From: Doug Anderson :      danderson-at-schnabel-eng.com
Date: Tue, 27 Jun 2000 13:23:17 -0400
Subject: LM - Vibration Criteria -- Anybody know of them?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello --

I have dug through the archives of this fine list and found a bunch of
information on vibration and microscopy. I work for an engineering
consulting company, and part of our work is related to vibration of various
sorts (implosions, blasting, construction, pile driving, vehicular
vibration). A current project is for a medical facility that is expanding,
including operating rooms that have surgical microscopes. The facility is
very close to a freight line, and we are working with them to provide
mitigation measures before the facility is constructed.

My question to the list is, does anybody know of actual vibration criteria
in terms of amplitude (peak or RMS) and frequency for ANY microscopes. The
microscope manufacturers we have contacted indicate no research in the area,
which is surprising to me.

Our previous work with both human perception and machine sensitivity has
usually involved some specific vibration criterion, whether it be for
damage, annoyance, or whatever purpose. The discussion of the various
isolation tables on the list is interesting, including the innertubes and
tennis balls. They appear to be a "One isolator fits all" approach, which,
if it works, is also surprising to me. Any help or leads in this regard
would be greatly appreciated. Thanks

Doug Anderson

Douglas A. Anderson, PhD
Senior Consultant
Schnabel Engineering Associates (http://www.schnabel-eng.com)
510 East Gay Street
West Chester, PA 19380
Phone: 610 696-6066, Fax: 610 696-7771




From daemon Tue Jun 27 18:12:10 2000



From: Arrowood, Roy :      arrowood-at-utep.edu
Date: Tue, 27 Jun 2000 11:21:06 -0600
Subject: coin question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Brian, that's a very intriguing problem. What is the metal or alloy of the
coin? What is the particular date you are dealing with? Could a
counterfeiter have done something so simple as cutting out segments of a
pre-existing numeral to alter the date? If so, perhaps channelling would
reveal the coining deformation pattern below the missing segments.

Also, is it clear that the WHOLE COIN is not a counterfeit? Ordinarily, the
effort and cost to create a die set for counterfeiting a whole coin would
deter anyone from the effort, unless the coin is spectacularly valuable. On
the other hand, if someone had a genuine coin (or a carefully modified one)
to use as a pattern, it would not be too dreadfully difficult (even a
century ago) to make a ceramic mold directly, or a metal die indirectly,
from the pattern. Then you could produce "knock-offs" from that die. And
your "real McCoy" could be undamaged. This would take a clever, patient, and
very meticulous craftsman to pull it off, however.

====================================
Roy Arrowood, Associate Professor
Metallurgical and Materials Engineering
UTEP, El Paso, TX 79968-0520
(915)747-6934
NEW E-MAIL ADDRESS: arrowood-at-miners.utep.edu



From daemon Tue Jun 27 18:12:11 2000



From: Philip Oshel :      peoshel-at-facstaff.wisc.edu
Date: Tue, 27 Jun 2000 12:59:24 -0500
Subject: Re: SEM: Mounting Fly Heads

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


This worked for amphipod maxilla, which are minute or smaller and
setose, so it should work for fly heads:
1) mount on double-sticky carbon-conductive tabs; cut these from a
sheet, or buy as stub-sized tabs
2) sharpen toothpicks or other sticks, some to a point and others to
a pen nib (with or without the slot found in a fountain pen nib)
3) place a small blob of Ag paint at the edge of the stub connecting
the surface of the sticky tab to the metal of the stub; I prefer Ag
paint dissoved in methylethylketone
4) use the stick to draw the paint from the blob to the fly head
(etc.); connect this to a part of the head that you don't care about;
draw a circle around the head just far enough not to touch the head
-- this creates a shorter conductive path from the specimen to the Ag
paint

Careful use of the sticks and paint will allow you to use Ag paint on
very small, hairy specimens. The paint can be allowed to partially
dry (solvent to evaporate) to thicken it, but this may just form a
skin on the surface, and does lead to stringing of the partly dry
paint which can be very annoying.

Phil

} An investigator just brought me 6 samples - heads of flies. He was
} instructed to bring the entire body but he cuts the heads off when
} he does TEM. The investigator knows nothing about SEM. I have done
} alot of scanning of fly eyes but the body is attached.
} My question is: how do I mount the heads on the stubs without the
} graphite or silver paint causing capillary action over the entire
} head? We will scan on a JEOL 840A after Au/Pd coating.
}
} George Lawton
} Chief Electron Microscopist
} Molecular and Cellular Imaging Facility
} UT Southwestern Medical Center at Dallas
} 5323 Harry Hines Blvd.
} Dallas, Tx 75390-9039
} Phone: 214-648-7291
} Fax 214-648-6408
} eMail: George.Lawton-at-email.swmed.edu

--
}}}}}}}}}}}}}}}}}{{{{{{{{{{{{{{{{{
Philip Oshel
Supervisor, AMFSC and BBPIC
Dept. of Animal Health and Biomedical Sciences
University of Wisconsin
1656 Linden Drive
Madison, WI 53706-1581
voice: (608) 263-4162
fax: (608) 262-7420 (dept. fax)



From daemon Tue Jun 27 18:12:12 2000



From: Richard E. Edelmann :      edelmare-at-casmail.muohio.edu
Date: Tue, 27 Jun 2000 15:33:59 -0500
Subject: Microscopy and Molecular Biology Postdoctoral Position Available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




{color} {param} 0100,0100,0100 {/param} Please respond directly to Dr. David Pennock at {/color} pennocdg-at-muohio.edu. {bold} {FontFamily} {param} TIMES {/param} {/bold} {color} {param} 0100,0100,0100 {/param} {FontFamily} {param} Arial {/param}



=======================================================


{bold} {bigger} {bigger} Microscopy and Molecular Biology Postdoctoral
Position

Department of Zoology

Miami University

Oxford, OH {/bold} {FontFamily} {param} TIMES {/param}


{FontFamily} {param} Arial {/param} A two-year postdoctoral position is available for
investigating the role of inner arm dyneins in ciliary motility.
We have seven different inner arm dynein heavy chain
genes cloned from {underline} Tetrahymena {/underline} {underline} thermophila {/underline} and are in
the process of creating knockout mutations in those
genes. The successful applicant will be involved in all
aspects of the work, including creation of the knockout
mutants and analysis of the phenotypes. The successful
candidate should be experienced in light and electron
microscopy and willing to learn molecular biology and
some protein biochemistry (preferred) or experienced in
molecular biology and protein biochemistry and willing to
learn light and electron microscopy. Send letter,
curriculum vitae, and reference letters to:


David Pennock

Department of Zoology

Miami University, Oxford, OH 45056


Email: pennocdg-at-muohio.edu {FontFamily} {param} TIMES {/param} {smaller} {smaller}



{/x-rich}



From daemon Tue Jun 27 18:12:12 2000



From: Steve Chapman :      PROTRAIN-at-CompuServe.COM
Date: Tue, 27 Jun 2000 15:38:04 -0400
Subject: SEM: Mounting Fly Heads

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


HI

Drill small holes in stubs and glue a dress making pin in each hole spike
upwards. Coat this in a sputter coater.

Push each insect head onto a pin and sputter coat at a specimen-target
distance of 5cms.

This technique is used by a number of our clients.

Steve Chapman
Senior Consultant Protrain
For consultancy and professional training in EM world wide.
www.emcourses.com
Tel 44+ 1280 814774 Fax 44+ 1280 814007



From daemon Tue Jun 27 18:12:16 2000



From: Matthew Lynn :      mlynn-at-miami.edu
Date: Tue, 27 Jun 2000 16:54:06 -0400
Subject: RE: LM geological stain question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Dee,

I've done a little work with varves...let me start by saying that typically
this imaging is done using X-rays or by making conventional thin sections for
petrographic microscopy. That said, I vaguely recall staining carbonate thin
sections with Alizarin Red S. There is a different stain for dolomite. I'm
not sure how well the technique can be applied to large sections....are they
still wet? Dehydrated? Vacuum impregnated? Let me know if this is on the
right track and I will hunt for some references.

Matt

Matthew J. Lynn, Ph.D.
Center for Advanced Microscopy
University of Miami
(305)284-4736
mlynn-at-miami.edu


On Tuesday, June 27, 2000 10:21 AM, Dee Breger [SMTP:micro-at-ldeo.columbia.edu]
wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear colleagues,
}
} I've received a query from a climatologist who wants to photograph fine
} varves and/or laminations (~0.5 mm- 1 mm each layer) in carbonate (calcite)
} rich silt (or marl) through a binocular light microscope. The samples are
} flat, finely cut or polished sections that can be etched if necessary, but
} he's really looking for a staining method that would enhance the difference
} between the carbonate-rich layers and the clay-rich layers to make them
} more usefully photogenic. Can anyone advise us on how to proceed to best
} advantage?
}
} Many thanks,
} Dee
}
}
}
}
} ***************************************************************
} Dee Breger
} Mgr. SEM/EDX Facility
} Lamont-Doherty Earth Observatory
} 61 Route 9W
} Palisades, NY 10964 USA
} T: 914/365-8640
} F: 914/365-8155
}
} http://www.ldeo.columbia.edu/micro
} http://www.discovery.com/area/science/micro/micro1.html
} http://www.lsc.org/antarctica/front.html
} Journeys in Microspace (Columbia University Press, 1995)
}



From daemon Tue Jun 27 18:12:18 2000



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Tue, 27 Jun 2000 15:01:49 -0700
Subject: Re: EDS on SEM w/o specimen airlock

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Jim,
We have a Kevex Quantum light element EDX on a Hitachi that vents the whole
chamber and it has been no problem, but I am told that the rate of venting
can make quite a difference. I bought our EDX detector used and the previous
owner claimed to have replaced the window every year for the five years that
he had it. I have never had to replace the window and that is in a student
lab. This particulars SEM takes over a minute to vent and makes no
discernable sound, whereas my other SEM vents in ten seconds with an audible
hiss. It, fortunately, has a Be-window detector, which is definitely
tougher. If your SEM vents quickly, you might try to put some sort of
restricter or filter on the vent to clean and slow it down.
At 10:59 AM 6/27/00 -0300, you wrote:
}
} Hi all,
}
} We're in the process of acquiring an EDS system for our JEOL 5600 and
} I'm curious about the experience of others with EDS systems on scopes
} without specimen airlocks (column vents to exchange specimens). Any
} thoughts/recommendations? I'm particularly concerned with contamination,
}
} window integrity and the like. Are there any advantages/disadvantages of
}
} Be vs. thin window detectors here?
}
} Thanks,
}
} Jim Ehrman
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca



From daemon Tue Jun 27 18:12:21 2000



From: Jane Fagerland :      jane.a.fagerland-at-abbott.com
Date: Tue, 27 Jun 2000 17:22:05 -0500
Subject: Research Cellular/Molecular Biologist - job announcement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


A colleague of mine has asked me to post the follwoing job announcement for
an opening in our Cancer Research area. Please send resumes to the address
at the bottom of the ad, or visit the website, which is also at the bottom of
the ad. Please do not contact me directly concerning this position. Thanks!

Jane A. Fagerland, Ph.D.
Dept. Microscopy and Microanalysis
Abbott Laboratories

******************************************************************************
******

ABBOTT LABORATORIES
PHARMACEUTICAL PRODUCTS DIVISION
Abbott Park, Illinois

Abbott Laboratories is a global diversified company dedicated to the
discovery, development, manufacture and marketing of health care products and
services. Around the world, the company,s 54,000+ employees are committed to
improving people,s lives by providing cost-effective health care
technologies. We are interested in interviewing candidates for the following
opportunity:

Research Cellular/Molecular Biologist

Participate in understanding the mechanism of action of novel anti-cancer
drugs with our in vivo oncology team. Areas of research include tumor
growth, angiogenesis, apoptosis and metastasis. Major responsibilities will
include immunohistochemistry, immunocytochemistry, in situ hybridization and
cellular analysis of tumor sections. Requires a Master,s degree with 3-5
years industrial or academic research experience. Must have experience in
immunohistochemistry, immunocytochemistry, in situ hybridization, tissue
preparations, general histology and cell cycle analysis. Experience with
cellular in vitro assays, tissue culture, western analysis and some molecular
biology techniques are desired. Requires good interpersonal and
communication skills.

Please send resumes to: 100 Abbott Park Road, D-583, AP9A, Abbott Park,
Illinois 60064 Attn. Job Code 2K-KDA3422. Or visit our web site at
www.abbott.com

An EOE, we are committed to employee diversity



From daemon Tue Jun 27 18:12:23 2000



From: James Talbot :      jptkt-at-flash.net
Date: Tue, 27 Jun 2000 18:08:58 -0500
Subject: LM geological stain question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dee-

Before staining, try using a UV light source to see if the calcite
fluoresces - can fluoresce red to pink, orange, white, yellow or blue.

If you need to stain, use Alizarin Red S for staining. If it turns a deep
red color, it's most likely calcite. Procedures for this can be found in
"Laboratory Handbook of Petrographic Techniques" by Charles S. Hutchinson,
1974, John Wiley and Sons. Or you can have a thin section preparation lab
do the staining for you - this will be much easier since they are set up to
do this routinely.

Cheers,
James Talbot

K/T GeoServices, Inc.
X-ray diffraction petrologic studies
visit my web site at http://www.ktgeo.com
Argyle, TX, USA, (214) 403-6342

} ----- Original Message -----
} From: Dee Breger {micro-at-ldeo.columbia.edu}
} To: {microscopy-at-sparc5.microscopy.com}
} Sent: Tuesday, June 27, 2000 9:20 AM
} Subject: LM geological stain question
}
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Dear colleagues,
} }
} } I've received a query from a climatologist who wants to photograph fine
} } varves and/or laminations (~0.5 mm- 1 mm each layer) in carbonate
} (calcite)
} } rich silt (or marl) through a binocular light microscope. The samples
are
} } flat, finely cut or polished sections that can be etched if necessary,
but
} } he's really looking for a staining method that would enhance the
} difference
} } between the carbonate-rich layers and the clay-rich layers to make them
} } more usefully photogenic. Can anyone advise us on how to proceed to
best
} } advantage?
} }
} } Many thanks,
} } Dee
} }
} }
} }
} }
} } ***************************************************************
} } Dee Breger
} } Mgr. SEM/EDX Facility
} } Lamont-Doherty Earth Observatory
} } 61 Route 9W
} } Palisades, NY 10964 USA
} } T: 914/365-8640
} } F: 914/365-8155
} }
} } http://www.ldeo.columbia.edu/micro
} } http://www.discovery.com/area/science/micro/micro1.html
} } http://www.lsc.org/antarctica/front.html
} } Journeys in Microspace (Columbia University Press, 1995)
} }
} }
} }
} }
}



From daemon Tue Jun 27 18:52:31 2000



From: Gib Ahlstrand :      giba-at-puccini.cdl.umn.edu
Date: Tue, 27 Jun 2000 18:55:06 -0500 (CDT)
Subject: Re: SEM: Mounting Fly Heads

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


George,

I've mounted small insects similar to what you now have using the following
method:

1. Get some dissecting pins, preferably, or any very thin, rigid pin or wire and
cut about 1/4 inch long.

2. Mount it vertically on an SEM stub using carbon paint, or any quick drying
glue, and let it dry completely, put in warming oven to speed it up, or use hair
dryer.

3. Position the fly heads upsidedown on clean surface, like lens tissue or glass
petri dish.

4. Put a fresh drop of carbon paint, or glue, on a nearby surface, pick up the
SEM stub with stub handling forceps, dip the tip of the pin into the fresh paint
or glue just enough to get a tiny little blob on the pin head, then under a
dissecting or low power scope touch the tip of the pin to the back surface of
the fly head. It should stick and you can pick it up at that point, place right
side up and let dry completely.

5. Coat in vacuum evaporator or sputter coater as usual. After coating run, vent
gas into chamber slowly to not disturb delicate sample.

Its a bit of a prep, but you also get the fly head off the stub surface, little
or no background junk in the view. Good luck!

Gib



Responding to the message of {s9587bef.020-at-mednet.swmed.edu}
from "George Lawton" {George.Lawton-at-email.swmed.edu} :
}
} An investigator just brought me 6 samples - heads of flies. He was
} instructed to bring the entire body but he cuts the heads off when he does
} TEM. The investigator knows nothing about SEM. I have done alot of scanning
} of fly eyes but the body is attached.
} My question is: how do I mount the heads on the stubs without the graphite
} or silver paint causing capillary action over the entire head? We will scan
} on a JEOL 840A after Au/Pd coating.
}
} George Lawton
} Chief Electron Microscopist
} Molecular and Cellular Imaging Facility
} UT Southwestern Medical Center at Dallas
} 5323 Harry Hines Blvd.
} Dallas, Tx 75390-9039
} Phone: 214-648-7291
} Fax 214-648-6408
} eMail: George.Lawton-at-email.swmed.edu
}
}
} .


Gib Ahlstrand
Electron Optical Facility, University of Minnesota, Dept. Plant Pathology
495 Borlaug Hall, St. Paul, MN. USA. 55108 (612)625-8249
612-625-9728 FAX, giba-at-puccini.cdl.umn.edu
http://biosci.umn.edu/MIC/consortium.html



From daemon Wed Jun 28 07:49:18 2000



From: Ron Doole :      ron.doole-at-materials.oxford.ac.uk
Date: Wed, 28 Jun 2000 08:11:38 +0100 (BST)
Subject: Re: EDS on SEM w/o specimen airlock

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All,

Just a reminder while we are on the subject of thin window detectors -
as well as controlling the gas flow on venting also make sure that the
chamber can not overpressure.
On SEMs the chamber door should be able to open freely as soon as it
reaches atmospheric pressure. On TEMs an aperture mechanism or similar
should be freed so that it can release at atmospheric pressure.

Ron



On Tue, 27 Jun 2000, Mary Mager wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear Jim,
} We have a Kevex Quantum light element EDX on a Hitachi that vents the whole
} chamber and it has been no problem, but I am told that the rate of venting
} can make quite a difference. I bought our EDX detector used and the previous
} owner claimed to have replaced the window every year for the five years that
} he had it. I have never had to replace the window and that is in a student
} lab. This particulars SEM takes over a minute to vent and makes no
} discernable sound, whereas my other SEM vents in ten seconds with an audible
} hiss. It, fortunately, has a Be-window detector, which is definitely
} tougher. If your SEM vents quickly, you might try to put some sort of
} restricter or filter on the vent to clean and slow it down.
} At 10:59 AM 6/27/00 -0300, you wrote:
} }
} } Hi all,
} }
} } We're in the process of acquiring an EDS system for our JEOL 5600 and
} } I'm curious about the experience of others with EDS systems on scopes
} } without specimen airlocks (column vents to exchange specimens). Any
} } thoughts/recommendations? I'm particularly concerned with contamination,
} }
} } window integrity and the like. Are there any advantages/disadvantages of
} }
} } Be vs. thin window detectors here?
} }
} } Thanks,
} }
} } Jim Ehrman
} Regards,
} Mary
}
} Mary Mager
} Electron Microscopist
} Metals and Materials Engineering
} University of British Columbia
} 6350 Stores Road
} Vancouver, B.C. V6T 1Z4
} CANADA
} tel: 604-822-5648
} e-mail: mager-at-interchg.ubc.ca
}
}
}

===========================================================================
Mr. Ron Doole e-mail ron.doole-at-materials.ox.ac.uk
Department of Materials, phone +44 (0) 1865 273701
University of Oxford, fax +44 (0) 1865 283333
Parks Road.
Oxford. OX1 3PH. UK.
============================================================================



From daemon Wed Jun 28 17:02:40 2000



From: Stephan Thiberge :      thiberge-at-wicc.weizmann.ac.il
Date: Wed, 28 Jun 2000 15:50:11 +0300
Subject: cathodoluminescent detector

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,

I want to buy a cathodoluminescent detector for a Scanning Electron Microscope JEOL JSM 6400.

I am looking for a company which has this product.

If you know such a company I would greatly appreciate your help.

Thanks
Stephan





From daemon Wed Jun 28 17:02:43 2000



From: COURYHOUSE-at-aol.com
Date: Wed, 28 Jun 2000 11:25:16 EDT
Subject: Re: Change of e-mail address and reminder of museum want list

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


OK thanks for the change of address, as usual we are looking for:
books on microscopes
catalogs of microscopes
microscope instruction manuals
books on application of microscopes either in biological or material sciences
.. anything! Optical, em sem, afm, etc etc etc.

-----
items and literature relating to any form of electrical communication and
engineering of thereof (yes telephone, telegraph, early wireless telegraphy
----
artifacts and literature on radio and TV broadcasting
---
Radar and radar countermeasures
-----
cryptanalysis
--------

thanks in advance as always
Ed Sharpe archivist for SMECC



From daemon Wed Jun 28 17:02:47 2000



From: Tom Phillips :      PhillipsT-at-missouri.edu
Date: Wed, 28 Jun 2000 12:20:51 -0500
Subject: scanning EM negative question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have used my Umax Powerlook III flatbed scanner with transparency
adapter many times to get high quality scans of my TEM negatives but
today it is driving me crazy. I have some great gold labeling of
pretty electron dense granules. On the negative I can clearly see
the gold against the granule matrix but when I scan, the 256 grey
levels compresses all that info into one level of black and therefore
I can't discern the gold. any scan gurus out there who can help? I
have tried playing with the gamma without much luck. thanks in
advance, tom

Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)



From daemon Wed Jun 28 17:02:51 2000



From: Greg Erdos :      gwe-at-biotech.ufl.edu
Date: Wed, 28 Jun 2000 14:20:13 -0400
Subject: Re: scanning EM negative question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Tom,
Have you tried telling the scanner that the image is a positive
transparency rather than a negative? And then reverse the contrast in
photoshop rather than with the scanner software.
Greg

At 12:20 PM 06/28/2000 -0500, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Gregory W. Erdos, Ph.D.
Assistant Director, Biotechnology Program
PO Box 110580
University of Florida
Gainesville, FL 32611

Ph. 352-392-1295 Fax: 352-846-0251



From daemon Wed Jun 28 17:02:54 2000



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Wed, 28 Jun 2000 10:32:35 -1000 (HST)
Subject: Re: scanning EM negative question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


we have a Epson 1600, sometimes this problem happens too. my solution is to
scan the negative in positive mode first then do a inversion after that.
Hope this helps.

Hao Li

----- Original Message -----
} From: "Tom Phillips" {PhillipsT-at-missouri.edu}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Wednesday, June 28, 2000 1:20 PM


Hi, Thomas

I find it best to scan a negative in as a positive transparency at 6
million colors (rather than the 256 greys you are using), and then reverse
it. This gives me the best tonal range.

Aloha,
Tina


} } I have used my Umax Powerlook III flatbed scanner with transparency
} } adapter many times to get high quality scans of my TEM negatives but
} } today it is driving me crazy. I have some great gold labeling of
} } pretty electron dense granules. On the negative I can clearly see
} } the gold against the granule matrix but when I scan, the 256 grey
} } levels compresses all that info into one level of black and therefore
} } I can't discern the gold. any scan gurus out there who can help? I
} } have tried playing with the gamma without much luck. thanks in
} } advance, tom
} }

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************



From daemon Wed Jun 28 17:02:55 2000



From: Larry Stoter :      LPS-at-teknesis.demon.co.uk
Date: Wed, 28 Jun 2000 21:08:13 +0100
Subject: Re: cathodoluminescent detector

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Oxford Instruments supply a wide range of CL detection systems, from
low cost broad-band detectors to multiple-band, UV, IR and
spectroscopic systems. As far as I am aware, they are the only
company supplying such a wide range of CL systems - if there are
others, sorry, and I would also like to know!

Regards
--
Larry Stoter
JEOL (UK) Ltd
Silver Court, Watchmead, Welwyn Garden City, AL7 1LT, United Kingdom
tel: +44-(0)1707-377117, fax: +44-(0)1707-373254, e-mail: larrys-at-jeoleuro.com



From daemon Wed Jun 28 17:02:55 2000



From: Tom Phillips :      PhillipsT-at-missouri.edu
Date: Wed, 28 Jun 2000 15:42:05 -0500
Subject: scanning EM negative problem solved

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thanks for all the suggestions. We did indeed find that scanning in
the color mode (which is 14 bit) solved the problem. I don't know
why UMax has their greyscale image only go to 256 colors. I will
ultimately incorporate this in a lecture when i am teaching image
analysis since it clearly shows the limitations of 8 bit images.
once again, thanks.

tom

Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)



From daemon Wed Jun 28 17:13:00 2000



From: Jonathan Barnard :      Jonathan.Barnard-at-angstrom.uu.se
Date: Wed, 28 Jun 2000 23:53:07 +0200
Subject: Re: SEM & CL

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Stephan,
I know that Oxford instruments can supply you with the necessary equipment
to do CL in the SEM. I am not sure whether this is standard equipment or
whether it is special order, but I have seen it done. The equipment usually
involves a parabolic mirror and a light-pipe with the relevant detector on
the end of this. Then there is the software .etc. There is a contact email
address on their web page:

http://www.oxford-instruments.com/

give them a try and see what they say.

Regards,
Jonathan



********************************************************
Dr Jonathan Barnard

Analytical Materials Physics
The Angstrom Laboratory, Uppsala University
P O Box 534, SE-751 21 Uppsala, Sweden
Phone: +46-(0)18-4716838 Fax: +46-(0)18-500131
Phone: Microscope room +46 18 471 6365
http://www.angstrom.uu.se/analytical/home.html
********************************************************



From daemon Wed Jun 28 17:13:01 2000



From: jmkrupp-at-cats.ucsc.edu (Jon Krupp)
Date: Wed, 28 Jun 2000 15:10:15 -0700
Subject: Have you tried MDS 100?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi:

Has anyone tried Kodak's MDS 100 imaging system? I have a user who needs a
quick documentation system and this might work for her. She is using NIH
Image on a compound scope in our lab and she wants to become independent
and do the work in her own lab, and maybe at other locations.

As an alternative, anyone with a Nubus frame grabber, ie Scion LG3 or
equiv., that would sell it to her to use with NIH Image?

Jonathan Krupp
Microscopy & Imaging Lab
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu




From daemon Thu Jun 29 07:56:07 2000



From: Caroline Schooley :      schooley-at-mcn.org
Date: Wed, 28 Jun 2000 16:52:54 -0700
Subject: Re: scanning EM negative question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} I have used my Umax Powerlook III flatbed scanner with transparency
} adapter many times to get high quality scans of my TEM negatives but
} today it is driving me crazy. I have some great gold labeling of
} pretty electron dense granules. On the negative I can clearly see
} the gold against the granule matrix but when I scan, the 256 grey
} levels compresses all that info into one level of black and therefore
} I can't discern the gold. any scan gurus out there who can help? I
} have tried playing with the gamma without much luck. thanks in
} advance, tom
}
} Thomas E. Phillips, Ph.D.
} Associate Professor of Biological Sciences
} Director, Molecular Cytology Core Facility
}
Tom -

You can get a really good book on scanner use at www.scantips.com or
download it as a pdf,if you have the patience for 218 pp.

Caroline

Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html




From daemon Thu Jun 29 07:56:15 2000



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Thu, 29 Jun 2000 16:05:07 GMT+1200
Subject: JEOL 840A cooling water flow

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi

Can anyone advise what the water flows should be in the three
parallel branches (OL; DPs; and power boards) of the 840A)
The manual spec is } 5 l/min total, but I need to know particularly
what the flow should be thru the OL.

TIA

rtch

ps replies welcome from JEOL


Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand



From daemon Thu Jun 29 07:56:17 2000



From: Stafford McKnight :      s.mcknight-at-ballarat.edu.au
Date: Thu, 29 Jun 2000 15:49:10 +1000
Subject: Cathodoluminescence detector

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Stephan,

We have an Oxford MiniCL detector attached to a JEOL 6300 -
same column as the 6400. This is an inexpensive detector which
suits our needs but there are other suppliers.
No matter who you obtain one from, give some thought to what
ports are free and to your analytical working distance (presume you
have EDS or WDS). On the 6400 this is most likely 15mm - some
ingenuity from Oxford or other supplier will be needed to clear the
pole-piece eg an inclined configuration. It would be worth it - rather
than have to alter the sample height for quant analysis.

Regards

Stafford
Stafford McKnight
Geology & Metallurgy
University of Ballarat
ph 03 53279262
fax 03 53279144



From daemon Thu Jun 29 07:56:18 2000



From: DavidSu-at-aol.com
Date: Thu, 29 Jun 2000 02:06:30 EDT
Subject: SEM Positions Open

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Accurel Systems International is looking for SEM Analysts: junior to
experienced technicians and engineers

Responsibilities include all aspects of commercial SEM and EDX analysis
(instrument operation, sample preparation, customer contact and follow-up,
data interpretation etc.).

Knowledge of IC device structure, IC fabrication and packaging a plus.

2+ years hands on experience in the semiconductor industry preferred.

To find out more please check our web site: www.accurel.com

SEM candidates should send resume with references to Regina Campbell:
reginac-at-accurel.com or FAX 408-737-3916.



From daemon Thu Jun 29 07:56:20 2000



From: richard.beanland-at-gecm.com
Date: Thu, 29 Jun 2000 09:15:09 +0000 (GMT)
Subject: subscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Subscribe Microscopy richard.beanland-at-gecm.com



From daemon Thu Jun 29 07:56:21 2000



From: richard.beanland-at-gecm.com
Date: Thu, 29 Jun 2000 11:04:31 +0000 (GMT)
Subject: Position available at Caswell Technology

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Microscopist/engineer:
Structural analysis department,
Marconi Caswell Ltd.,
Towcester,
Northants NN12 8EQ
UK

Job description:
You will form part of a team of specialists in analytical techniques, providing support to the rapidly growing III-V optoelectronic and microwave device fabrication plant at Caswell Technology. We perform a wide range of activities, including optical microscopy, SEM, TEM, EDX, X-ray techniques and scanning probe microscopies. You will be expected to be able to be competent in several of these techniques and have/develop a degree of expertise in one or more of them. Experience of semiconductor device fabrication techniques would be an advantage.

The post is available immediately.

More details of our company are available at
http://www.caswelltechnology.com/

Please contact me if you are interested or would like any further information.

==============================================================
Richard Beanland
Caswell Technology,
Caswell,
Towcester,
Northants NN12 8EQ

e-mail richard.beanland-at-gecm.com
Tel. +44 1327 356363
Fax. +44 1327 356398
http://www.caswelltechnology.com/
==============================================================



From daemon Wed Jun 28 17:02:44 2000



From: Nestor J. Zaluzec :      zaluzec-at-aaem.amc.anl.gov
Date: Wed, 28 Jun 2000 11:23:51 -0500
Subject: Administrivia: Change of Address Notices

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Folks

Don't broadcast change of address notices to the Listserver.
If you want to change your subscription address, just send
a unsubscribe message, followed by a subscribe message...

BUT SEND IT TO THE ADMINISTRATIVE ADDRESS!!!!

LISTSERVER-at-MSA.MICROSCOPY.COM

don't send them to the posting address

(Microscopy-at-MSA.MICROSCOPY.COM)

Nestor
Your Friendly Neighborhood SysOp

==================================================================
Nestor J. Zaluzec
Materials Science Division
Building 212
Argonne National Lab
9700 S. Cass Ave
Argonne, Illinois 60439 USA
Tel: 630-252-7901, Fax: 630-252-4798
Email: Zaluzec-at-aaem.amc.anl.gov
==================================================================
TPMLab: http://tpm.amc.anl.gov
MMSite: http://www.amc.anl.gov
==================================================================

The box said "This program requires Win 95/98/NT or better..." so I bought
a G3 Mac

==================================================================




From daemon Thu Jun 29 08:07:21 2000



From: richard black :      m02jmy00-at-cwcom.net
Date: Thu, 29 Jun 2000 07:58:15 -0500
Subject: Printer information please

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I run a microscopy unit which is completely digital. I have a problem
with printing out images. I am planning to upgrade cameras to 10 or 12
bit megapixel units, but am already restricted by printers. Laser
printers seem to deal with greyscales by halftoning at 100-150dpi which
is unsatisfactory for small prints. Are there any true greyscale
printers available, that manage better than 300dpi and better than 256
grey levels? Is there any advantage in having more than 256 grey
levels? Sorry if this is a bit dim, but there are so many self styled
experts who give conflicting opinions I end up totally confused.
Thank you.
Richard Black
Nottingham, England
richardblack-at-cwcom.net




From daemon Thu Jun 29 08:07:22 2000



From: Tim Lyden, Ph.D. :      lyden.11-at-osu.edu
Date: Thu, 29 Jun 2000 09:12:54 -0400
Subject: Scan site

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


This is my first message to the list... Caroline, I looked at Scan site and
would like to try PDF format... but I can seem to locate the pathway to
download? Any thoughts or pointers?

Thanks for the suggestion it looks like a great site.
Tim Lyden, Ph.D.
Research Scientist

Departments of Internal Medicine/Immunology and Physiology/Cell Biology
Ohio State University
Davis Medical Research Center
480 W 9th Ave.
Columbus, Ohio 43210


Phone: 614-293-4867
Fax: 614-293-5631



From daemon Thu Jun 29 17:20:53 2000



From: Alesia Darling :      adarling-at-coe.eng.ua.edu
Date: Thu, 29 Jun 2000 09:10:34 CST
Subject: Tunneling Electron Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I would like to purchase a "Tunneling Electron Microscope".

I have been unable to locate a supplier. If you know of one please
let me know. Thanks!
Alesia White Darling
Secretary, Dr. Amnon Katz
University of Alabama
Aerospace Engineering and Mechanics
Box 870280/205 Hardaway Hall
Tuscaloosa, Alabama 35487-0280
ph: 205-348-8525
fax: 205-348-7240 or 205-348-2094



From daemon Thu Jun 29 17:20:53 2000



From: Soumitra Ghoshroy :      ghoshroy-at-nmsu.edu
Date: Thu, 29 Jun 2000 07:13:50 -0700
Subject: Re: scanning EM negative question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

I have found that the gold does not show up well when you scan the negative
itself. So for gold to show up in the scanned image, I usually make one
print and scan the print. This works quite well. I guess we will still have
to work in darkrooms for quite sometime even though the digital imaging is
becoming popular.

Good luck,

Soumitra

}
}
} I have used my Umax Powerlook III flatbed scanner with transparency
} adapter many times to get high quality scans of my TEM negatives but
} today it is driving me crazy. I have some great gold labeling of
} pretty electron dense granules. On the negative I can clearly see
} the gold against the granule matrix but when I scan, the 256 grey
} levels compresses all that info into one level of black and therefore
} I can't discern the gold. any scan gurus out there who can help? I
} have tried playing with the gamma without much luck. thanks in
} advance, tom
}
} Thomas E. Phillips, Ph.D.
} Associate Professor of Biological Sciences
} Director, Molecular Cytology Core Facility
}
} 3 Tucker Hall
} Division of Biological Sciences
} University of Missouri
} Columbia, MO 65211-7400
} (573)-882-4712 (voice)
} (573)-882-0123 (fax)




*****************************************************************
Soumitra Ghoshroy Ph.D.
Electron Microscopy Lab
Box 3EML
New Mexico State University
Las Cruces, NM 88003
Tel: 505-646-3600
Fax: 505-646-5665
e-mail: ghoshroy-at-nmsu.edu
http://confocal.nmsu.edu/eml




From daemon Thu Jun 29 17:20:57 2000



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Thu, 29 Jun 2000 11:21:33 -0500
Subject: Re: Printer information please

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


As I recall, the human eye is hard pressed to distinguish more than 6-bit
grayscale (64 levels) unless the shades are right next to each other, which
they might be in an image. Therefore, printing at 256 gray levels should be
more than adequate.

The 10 or 12-bit cameras will still be handy in storing the extra
information in your images. You will be able to do some gray scale
manipulation to highlight the darker or lighter regions of your image. But
when it comes to print time, you will be throwing out all but about 8 bits.

You rightly say that halftoning is a problem on small prints. You need a
16x16 dot cell to render 256 gray levels in halftone. For 1200 dpi
printing, that means that you can achieve
75 pixels per inch (not very high). That means a 1024 pixel image would
require 13.65 inches to show all that resolution. If the image is printed
out smaller, then either grayscale or pixel resolution would be sacrificed.
(Printing at 64 gray levels would require a 8x8 cell and could be done at
150 pixels per inch so that the image could fit into 6.8 inches.)

I don't have a dye sub printer here, but their specs would probably meet
your needs. You would want to see how well they can render the grayscale.
Since they do not halftone, the dpi spec would be the same as the pixel per
inch spec. A 300 dpi printer should be able to render full resolution on a
1024 pixel image in 3.4 inches. But check out the grayscale performance to
see if it is adequate.

Warren S.

At 07:58 AM 6/29/2000 -0500, you wrote:

} I run a microscopy unit which is completely digital. I have a problem
} with printing out images. I am planning to upgrade cameras to 10 or 12
} bit megapixel units, but am already restricted by printers. Laser
} printers seem to deal with greyscales by halftoning at 100-150dpi which
} is unsatisfactory for small prints. Are there any true greyscale
} printers available, that manage better than 300dpi and better than 256
} grey levels? Is there any advantage in having more than 256 grey
} levels? Sorry if this is a bit dim, but there are so many self styled
} experts who give conflicting opinions I end up totally confused.
} Thank you.
} Richard Black
} Nottingham, England
} richardblack-at-cwcom.net



From daemon Thu Jun 29 17:21:02 2000



From: Lehman, Ann :      Ann.Lehman-at-trincoll.edu
Date: Thu, 29 Jun 2000 14:02:38 -0400
Subject: Balzers Contact Info

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I think this query was posted recently but I haven't been able to dredge up
the replies in the list archives - too recent, maybe.

I'm looking for contact info for a colleague, for Balzers or Bal-tec. He
inherited a B. carbon evaporator model CED 030 (also: 3U-G03-751/132). Can
anyone help out with either contact info for the company, or a copy of the
manual?? We will pay copy/ship charges.

Thanks, much.

Ann Hein Lehman
Manager, EM Facility
Trinity College
Hartford CT 06106
v. 860-297-4289
f. 860-297-2538
e. ann.lehman-at-trincoll.edu



From daemon Thu Jun 29 17:21:02 2000



From: =shAf= :      mshaf-at-darkwing.uoregon.edu
Date: Thu, 29 Jun 2000 14:47:18 -0700
Subject: SEM: LaB6-to-anode distance

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Hai Li:
Here is some references which claim MgAl2O4 is a
good substrate for growing perovskites:

Miura, S., Yoshitake, T., Matsubara, S., Miyasaka, Y., Shohata, N.
and Satoh, T., Epitaxial Y-Ba-Cu-O Films on Si with Intermediate
Layer by RF Magnetron Sputtering, Appl. Phys. Lett. 53:1967-1969
(1988).

Wu, X.D., Inam, A., Hegde, M.S., Wilkens, B., Chang, C.C., Hwang,
D.M., Nazar, L., Venkatesan, T., Miura, S., Matsubara, S., Miyasaka,
Y. and Shohata, N., High Critical Currents in Epitaxial YBa2Cu3O7-x
Thin Films on Silicon with Buffer Layers, Appl. Phys. Lett.
54:754-756 (1989).

My buddy, Darrell Schlom at Penn State tells me that using
perovskite substrates is much better:

Y. Jia, M.A. Zurbuchen, S. Wozniak, A.H. Carim, D.G. Schlom, L-N.
Zou, S. Briczinski, and Y. Liu, "Epitaxial Growth of Metastable
Ba2RuO4 Films with the K2NiF4 Structure," Applied Physics Letters 74
(1999) 3830-3832.

Best regards,
Mike Urbanik
www.crystalguru.com


} } Subj: information about MgAl2O4


For a tungsten emitter, my JEOL operators' manual implies to raise
the anode closer to the Wehnelt for low keV applications (less than
15keV). The manual does not mention this distance for the LaB6
emitter option. Any suggestions?

TIA and cheerios, =shAf= :o)

{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {}
Michael Shaffer, R.A. - mshaf-at-darkwing.uoregon.edu
Geological Science's Electron Probe Facility - University of Oregon
http://epmalab.uoregon.edu/




From daemon Fri Jun 30 07:23:58 2000



From: Sarah Lundberg :      lundberg-at-nevada.edu
Date: Thu, 29 Jun 2000 15:21:34 -0700
Subject: Re: Cathodoluminescence detector

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We also have an Oxford MiniCL detector (attached to our JEOL-8900), however
the Research Instruments group of Oxford (CL and Cryo EM stuff) was just
sold to Gatan, so you may need to contact Gatan to find out about the
product. We are in the middle of some modifications to our unit (extension
of the light pipe), and JEOL informed me of the transaction. It doesn't
appear on the websites, however it was official as of 6-19-00. Hope the
info helps.
Sarah

Stafford McKnight wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Stephan,
}
} We have an Oxford MiniCL detector attached to a JEOL 6300 -
} same column as the 6400. This is an inexpensive detector which
} suits our needs but there are other suppliers.
} No matter who you obtain one from, give some thought to what
} ports are free and to your analytical working distance (presume you
} have EDS or WDS). On the 6400 this is most likely 15mm - some
} ingenuity from Oxford or other supplier will be needed to clear the
} pole-piece eg an inclined configuration. It would be worth it - rather
} than have to alter the sample height for quant analysis.
}
} Regards
}
} Stafford
} Stafford McKnight
} Geology & Metallurgy
} University of Ballarat
} ph 03 53279262
} fax 03 53279144

--
Sarah A.W. Lundberg
Electron Microanalysis and Imaging Laboratory
Department of Geoscience, UNLV
4505 S. Maryland Parkway Box 454010
Las Vegas, NV 89154-4010

EPMA Lab (702) 895-2660
SEM Lab (702) 895-2462
Office (702) 895-1134
Fax (702) 895-4064
Dept. Office (702) 895-3262

lundberg-at-nevada.edu
http://www.unlv.edu/Colleges/Sciences/Geoscience/EMP.htm

Home:
8025 W. Russell Rd. Apt. #1117
Las Vegas, NV 89113
(702) 871-9635




From daemon Fri Jun 30 07:23:59 2000



From: Gordon Vrololjak :      gvrdolja-at-nature.Berkeley.EDU
Date: Thu, 29 Jun 2000 15:53:56 -0700 (PDT)
Subject: Re: Printer information please

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hellow Richard,
HP Laserjet 2100TN is used in our laboratory and it has a very high stated
resolution of 1200dpi. The printer gives excellent output at its highest
setting, and lower. The level of detail I obtain from the printer is
sufficient to show all the information from TEM images. However, I
believe the final output from a photograph is still slightly
better. Also, photographs have very nice glossy or matt finishes which
I haven't seen on laser printouts.

\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
Gordon Ante Vrdoljak Electron Microscope Lab
ICQ 23243541 http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
gvrdolja-at-nature.berkeley.edu UC Berkeley
phone (510) 642-2085 Berkeley CA 94720-3330
fax (510) 643-6207 cell (510) 290-6793

On Thu, 29 Jun 2000, richard black wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I run a microscopy unit which is completely digital. I have a problem
} with printing out images. I am planning to upgrade cameras to 10 or 12
} bit megapixel units, but am already restricted by printers. Laser
} printers seem to deal with greyscales by halftoning at 100-150dpi which
} is unsatisfactory for small prints. Are there any true greyscale
} printers available, that manage better than 300dpi and better than 256
} grey levels? Is there any advantage in having more than 256 grey
} levels? Sorry if this is a bit dim, but there are so many self styled
} experts who give conflicting opinions I end up totally confused.
} Thank you.
} Richard Black
} Nottingham, England
} richardblack-at-cwcom.net
}
}
}
}



From daemon Fri Jun 30 07:24:01 2000



From: Pbgrover-at-aol.com
Date: Thu, 29 Jun 2000 19:55:03 EDT
Subject: re: Balzers contact info

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ann,

Balzers stuff is now handled by a company called Techno Trade. If you can't
find them on the 'net or find their address, email me and I'll dig through my
files and find their address for you.

Cheers :o)

Paul Grover
Chief Microscopist and Bottle Washer
Microvista Laboratory
Lafayette, IN



From daemon Fri Jun 30 07:24:01 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Thu, 29 Jun 2000 17:30:47 -0700
Subject: Olympus 100X/1.4 PlanAPO available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have one new unit Olympus 100X/1.4 PlanAPO 160 tube length objective
for sale. This is a very rare objective to find.

Mine is new, unused. Perfect. List price is over $5,000 (whew).

Asking $3750. Pls decode anti-spam email or telecon me at
916.791.8191, fax at 916.791.8186.

gary g



From daemon Fri Jun 30 07:24:03 2000



From: Dr. Gary Gaugler :      gary-at-gaugler.com
Date: Thu, 29 Jun 2000 19:23:54 -0700
Subject: Re: SEM: LaB6-to-anode distance

Contents Retrieved from Microscopy Listserver Archives
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My Amray says the same thing. As long as the gun's Whenelt end
is the same distance for each emitter type, the spacing should
be the same. I used 4mm {=5KV, 6mm {=12KV and 8mm
for } 12KV.

gary g.


At 02:47 PM 6/29/00, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Fri Jun 30 07:24:05 2000



From: John Foust :      jfoust-at-threedee.com
Date: Thu, 29 Jun 2000 21:57:09 -0500
Subject: What makes up a phase contrast set?

Contents Retrieved from Microscopy Listserver Archives
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I'm selling a perhaps incomplete set of parts for a phase
contrast setup for a B&L microscope. I don't have any B&L
equipment, and it was part of a lot I got a sale....

http://cgi.ebay.com/aw-cgi/eBayISAPI.dll?ViewItem&item=369625459

What other components would've made up the entire phase contrast
set?

- John



From daemon Fri Jun 30 07:24:11 2000



From: Jintamas SUWANJARAT :      sjintama-at-ratree.psu.ac.th
Date: Fri, 30 Jun 2000 14:22:13 +0700 (GMT)
Subject: subscribe

Contents Retrieved from Microscopy Listserver Archives
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Dear Sir,
Please subscribe, thanks.



From daemon Fri Jun 30 07:24:15 2000



From: =?iso-8859-2?Q?Krzysztof_Jan_H=FCbner?= :      hubner-at-IOd.krakow.pl
Date: Fri, 30 Jun 2000 12:12:50 +0200
Subject: Conference "ADI - FOUNDRYS OFFER FOR DESIGNERS '

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


INTERNATIONAL SCIENTIFIC CONFERENCE

ADI - FOUNDRYS OFFER FOR DESIGNERS
AND USERS OF CASTINGS

23-24 NOVEMBER 2000
CRACOW – FOUNDRY RESEARCH INSTITUTE

CIRCULAR NO. 1

HOSTED BY:
Instytut Odlewnictwa
30-418 Kraków, Zakopiañska 73

CO-ORGANISER:
Ministry of Economy
Polish Foundrymen’s Technical Association
Foundry Economic Chamber
SCIENTIFIC COMMITTEE

List of persons invited to participation in the Scientific Committee of the
Conference:
Prof. Jerzy PIASKOWSKI – Poland
Prof. Czes³aw PODRZUCKI – Poland
Prof. Edward GUZIK – Poland
Prof. Jan R¥CZKA – Poland
Prof. Ryszard KOZ£OWSKI - Poland
Prof. Mieczys³aw KACZOROWSKI - Poland
Prof. Jouko.J. VUORINEN – Finland
Prof. Eduard DORAZIL – Czech Republic
Prof. Lubomir BECHNY – Slovakia
Prof. Jorge A. SIKORA - Argentina
dr Franco ZANARDI - Italy
dr Matti JOHANSON – Finland
dr Jose R. GUIRIDI - Spain
dr Konstantin UZ£OW - Ukraine

ORGANISING COMMITTEE
Dr Eng. Jerzy TYBULCZUK - Chairman
Dr Eng. Adam KOWALSKI – Secretary
Dr Eng. Józef TURZYÑSKI
M.Sc.Eng. Krystyna £USZCZKIEWICZ
Eng. Marta KONIECZNA
M.A. Krystyna BANY-KOWALSKA
M.Sc.Eng. Andrzej PYTEL
Eng. Janusz CUPIA£

PURPOSE AND SUBJECT OF CONFERENCE
The purpose of the Conference is to enable the national and foreign research
& development centres as well
as industrial units to present their latest achievements in developing the
heat treatment technology,
metallographic examinations, and quality control systems for high-grade
ductile iron - ADI in particular.

Therefore you are encouraged to submit your proposals of papers dealing with
the following subjects :
Foundry
New grades of ductile iron, their properties and technologies of production.
Methods of spheroidising and inoculation treatments
Metals science
Electron microscopy. X-ray phase analysis. Quantitative metallography. The
techniques of colour etching
and their application in metallography. New methods and tools to examine the
structure of ductile iron and
ADI.
Heat treatment
Methods of ductile iron heat treatment. Installations. Methods of heat
treatment control. Heat treatment
parameters. Technologies of ADI fabrication.
Quality
Quality systems according to ISO and EN Standards used in production of
ductile iron and ADI. Control of
production process. Statistical quality control.

PRESENTATION OF PAPERS
Papers will be presented during the plenary meeting (time for presentation
15-30 minutes).
Language of the Conference : English and Polish

INSTRUCTIONS FOR AUTHORS OF THE PAPERS
Paper volume : 6-8 pages including tables and figures on A4 paper. Even
number of pages.
Margins : left - 2.5 cm, right - 2.5 cm, upper - 2.5 cm, lower - 2.5 cm.
Write your text in editor Word 6 or Word 97, single line spacing, font Times
New Roman 12 pt.
The starting line of a new paragraph should be indented by 1 cm..
Figures, photographs and tables should run in the text of the paper.
Captions should be typed in bold and
centred.
Equations should be centred leaving one free spacing above and below the
equation.
The pages should be numbered consecutively using soft pencil.
References : published literature cited in the text should be quoted using a
number in square brackets.
Abstract : Please start with an abstract of up to 50 words.

SAMPLE OF PAPER FORMAT

On first page (only !) leave free space from the top of 10 single line
spacings

TITLE OF PAPER IN BOLD CAPITALS (centred, 14 pt)

space 2 x 1

Name & Surname (bold, centred, 12 pt)
Affiliation, e.g. Foundry Research Institute (italics, centred, 12 pt)
Place, e.g. Kraków

space 2 x 1
ABSTRACT (bold capitals, centred, 12 pt)
space 1 x 1
Text follows - approximately 50 words, indentation of 2.5 cm on the right
and left, 12 pt.

space 2 x 1
1. INTRODUCTION (bold capitals, 12 pt)
Text justified written in single line spacing.
2. FIRST SUBTITLE (bold capitals, 12 pt)
Text justified written in single line spacing.
2.1. Second subtitle (bold, 12 pt)
Text justified written in single line spacing.
space 3 x 1
REFERENCES (bold capitals, 12 pt)
OTHER EVENTS

during the Conference, the universities, industrial plants and companies
will have an opportunity to
display their products, research methods, manufacturing techniques and
computer programmes in the
form of short (up to 15 minutes) presentations and/or exhibitions - all
those who are willing to take part in
the display are kindly requested to agree in advance with the Organising
Committee the subject and form
of display,
the organisers of the Conference also offer the possibility of publishing
the ready advertising and
information materials in the Conference Proceeding upon previous agreement
with the Organising
Committee.

Details along with the Conference programme will be circulated early in
October 2000 in CIRCULAR NO.
2.

For more information please contact the following persons:

Adam KOWALSKI tel. (+48 012) 2618-502
Krystyna BANY-KOWALSKA tel. (+48 012) 2618-591
e-mail: awkowal-at-iod.krakow.pl

Address for correspondence :

INSTYTUT ODLEWNICTWA
Centrum Informacji Naukowo-Technicznej i Ekonomicznej, Normalizacji i
Szkolenia

ul. Zakopiañska 73,
30-418 Kraków, Poland

fax: +48 (012) 260 08 70
e-mail: awkowal-at-iod.krakow.pl


SCHEDULE
completed participation forms should be sent by 31 October 2000
technical papers for presentation during the Conference should be sent by 30
September 2000
proposals of promotion during the Conference should be sent by 15 October
2000


REGISTRATION FEE
(Note : accomodation is NOT included)
full rate: 200 $
reduced rate (for participants presenting papers and posters) 150 $
The cost of promotion is to be agreed.

Please make you cheque payable to :
BPH I Oddzia³ w Krakowie
Account No. : 10601376-320000033388

==========================================================================

International Conference
ADI - AN OFFER FOR DESIGNERS AND USERS OF CASTINGS.
23 - 24 November 2000


PARTICIPATION FORM

Family Name .......................................................

Surname ..............................................................

Title.......................... Post ..................................

Company :

Name ................................................................

Address ..............................................................

...........................................................................
Fax.......................E-mail......................................

I declare my participation in the Conference
and wish to submit a technical paper
Yes ? No ?

I wish to make promotion of my Company
Yes ? No ?

Title of paper ......................................................

..........................................................................

Author(s) ............................................................

Signature .............................................................
Please return the completed participation form to the following address:

INSTYTUT ODLEWNICTWA
Centrum Informacji Naukowo-Technicznej
i Ekonomicznej, Normalizacji i Szkolenia
ul. Zakopiañska 73, 30-418 Kraków, Poland







From daemon Fri Jun 30 07:24:16 2000



From: SGKCCK-at-aol.com
Date: Fri, 30 Jun 2000 04:26:25 EDT
Subject: M&M2000- Philadelphia

Contents Retrieved from Microscopy Listserver Archives
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Here in Philadelphia we are all looking forward to the upcoming M&M meeting
in August. The meeting promises to be the best yet. To make everyone's
arrival and traveling as pleasant as possible we recommend you visit the
Philadelphia Airport Web site at http://www.phl.org/. Here you will be able
to go directly to ground transportation and find the easiest and least
expensive ways to get from the airports to the hotels in the city. The ride
from the airport to the hotels is approximately 15-20 minutes. Depending on
the transportation you choose the prices will range from $8.00 on up.
Please feel free to E-mail me with any specific questions you may have and I
shall try to assist.
We look forward to seeing everyone in August and have a great trip.

Sincerely,

Stacie Kirsch
LAC Chair



From daemon Fri Jun 30 07:24:17 2000



From: Everett Ramer :      Everett.Ramer-at-netl.doe.gov
Date: Fri, 30 Jun 2000 07:34:48 -0400
Subject: Procedure for handling LN2

Contents Retrieved from Microscopy Listserver Archives
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I will getting my first SEM/EDS shortly and to obtain the required operating permit from my safety group I need a written procedure for handling LN2. Specifically I need a written procedure for filling the 3 L dewar on the EDS detector from a 50 L dewar mounted on a cart. I plan to make the transfer by using a lab source of N2 to pressurize the 50 L dewar and have obtained all the valves and fittings required to do this from another SEM lab. I would appreciate copies of the procedure.
Thanks,

Everett Ramer
National Energy Technology Laboratory
P.O. Box 10940, Cochrans Mill Road
Pittsburgh, PA, USA 15236-0940
Voice: 412-386-4920
FAX: 412-386-4806
ramer-at-netl.doe.gov



From daemon Fri Jun 30 07:24:17 2000



From: JHumenansky-at-phi.com
Date: Fri, 30 Jun 2000 06:48:35 -0500
Subject: Re: SEM: LaB6-to-anode distance

Contents Retrieved from Microscopy Listserver Archives
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The JEOL 840 that I am familiar with (this most likely applies to most JEOL
SEM's) sets the tip whenelt distance within a narrow range and as I recall
this distance is correct when the auto bias emission current is about
100ua. This makes it possible to fine tune the first cross over with the
manual bias control so that at 20kV the emission current would be adjusted
with the manual bias control to be about 8-9 and the emission current would
be about 10=20uA. The effects of this can be seen viewing the emission
pattern. The spot size will be smaller using a high number manual bias
number. If the auto bias emission current was say only 50-60uA when the
SEM was operated at 1kV accelerating voltage it would be more difficult to
fine tune the first cross over spot size because the bias number would be
about 0-1 and the emission current would be to low. Increasing the bias
number decreases the emission current. If the emission current in auto
bias is less than 100uA the whenelt is rotated slightly CW to shorten the
distance. Manual bias is the usual way to operate the LaB6 filament while
auto bias is usually used with a W filament.

Because the LaB6 source is much brighter than W filament, the first cross
over at the source can be fine tuned with the LaB6 cathode which results in
smaller spot size on the sample ie. better resolution with sufficient
current to have good signal to noise ratio.

If you use a W filament and try to set up manual bias the same way as you
would for LaB6 you will see that the signal to noise is worse using high
manual bias number (less emission current). The LaB6 filaments are
usually operated in manual bias while the W filaments are normally used
with auto bias.

On the 840 there are either two accelerating anodes, a high anode for low
kV and a low anode for high kV which is the case if you have an elctro
static beam blanker installed or a single anode that can be raised for low
kV or lowered for high kV operation. The anode in this case snaps into the
correct position. Hope this helps.

John Humenansky/Staff Scientist
Physical Electronics, Inc. (PHI)
6509 Flying Cloud Drive
Eden Prairie, MN 55344
952-828-6387



From daemon Fri Jun 30 12:15:21 2000



From: Leona Cohen-Gould :      lcgould-at-mail.med.cornell.edu
Date: Fri, 30 Jun 2000 09:04:58 -0400
Subject: Re: resin shelf life

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All,
I helped empty on old lab and came across a dozen never-opened bottles of
Epon-812 (yes, the real thing). You can well imagine how long they had sat
on the shelf. My question to you is....what do I do with it? My gut
reaction is to give it to our Life Safety guys to haul away, but if its
still good that would be a waste. Short of opening one of the bottles and
making a test batch, is there any rule of thumb out there?
The bottles have a WPE of 160. They are still in the original plastic
wrap and have an EMS label. Maybe Stacie has a thouhgt on this (if she's
not overwhelmed with planning for M&M).

Thanks in advance,
Lee

Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175




From daemon Fri Jun 30 12:15:22 2000



From: Angela Klaus :      avklaus-at-amnh.org
Date: Fri, 30 Jun 2000 10:44:24 -0400
Subject: Re: Balzers Contact Info

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Ann...

The Bal-Tec representative is TechnoTrade International. 603-622-5011.

Best,

Angela

At 02:02 PM 06/29/2000 -0400, you wrote:

} I think this query was posted recently but I haven't been able to dredge up
} the replies in the list archives - too recent, maybe.
}
} I'm looking for contact info for a colleague, for Balzers or Bal-tec. He
} inherited a B. carbon evaporator model CED 030 (also: 3U-G03-751/132). Can
} anyone help out with either contact info for the company, or a copy of the
} manual?? We will pay copy/ship charges.
}
} Thanks, much.
}
} Ann Hein Lehman
} Manager, EM Facility
} Trinity College
} Hartford CT 06106
} v. 860-297-4289
} f. 860-297-2538
} e. ann.lehman-at-trincoll.edu

---------------------------------------------
Angela V. Klaus

Director, Core Imaging Facility
American Museum of Natural History
Central Park West at 79th Street
New York, NY 10024-5192 USA

Email: avklaus-at-amnh.org
Voice: (212)769-5977
Fax: (212)496-3480
---------------------------------------------



From daemon Fri Jun 30 12:15:23 2000



From: Greg Erdos :      gwe-at-biotech.ufl.edu
Date: Fri, 30 Jun 2000 11:42:19 -0400
Subject: Re: resin shelf life

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have used Epon-812 that is over 25 years old with no problem. This is
valuable stuff so I hope you do not through it out. Somebody out there
will surely take it off your hands. I have enough to last me the rest of
my career

At 09:04 AM 06/30/2000 -0400, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Gregory W. Erdos, Ph.D.
Assistant Director, Biotechnology Program
PO Box 110580
University of Florida
Gainesville, FL 32611

Ph. 352-392-1295 Fax: 352-846-0251



From daemon Fri Jun 30 16:59:30 2000



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Sat, 30 Mar 1996 07:57:02 +0000
Subject: Re: Procedure for handling LN2

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi, Everett. I guess I am glad I am not working for a government anymore.
Otherwise I would probably be having to fill out similar forms. It would be
nice if the folks that review those forms knew what the issues were. I am
always afraid that they will know just enough to be dangerous and may
actually require some unhealthy procedures. But perhaps they are sensible
and only require that you have something officially in writing to document
your best practice.

I found the following post in my files from 4 years ago. I hope Larry does
not mind me reposting it. His conclusions are quite interesting.

Warren S.



} Wil's recent comment on the safety hazards of distilled water brought to
} mind some peculiar safety regulations here in MD. In reference to liquid N
...
} officer listening in will recommend new safety procedures requiring
} protective booties!
} In the end, we can't legislate common sense, nor can we abdicate
} responsibility to those above.

Try getting your safety officer to conduct an experiment:

1. Hold out hand,
2. Pour a small volume of liquid N2 over hand
3. Now the interesting bit - put on a glove, and pour the same quantity of
liquid N2 into glove.
4. Phone for ambulance.

The point is that a brief contact causes no problems, but if the contact is
continued you get a nasty burn.

Gloves, goggles, masks (and shoes) are actually more dangerous when handling
liquid N2 than sandals and no protection. And clothes are actually more
dangerous than being naked. Get the safety officer to experiment. With a
little persuasion you can probably convince the safety officer that when
handling liquid N2, everybody should be naked.

More seriously, bureaucrats, administrators and the inexperienced should
talk to somebody who has real knowledge.

--------------------------------------------------------------
Dr. Larry Stoter
Technesis
17, Rocks Park Road, Uckfield, E. Sussex, TN22 2AT, United Kingdom
Larry-at-teknesis.demon.co.uk
--------------------------------------------------------------



From daemon Fri Jun 30 16:59:32 2000



From: Kristen Lennon :      kalen-at-citrus.ucr.edu
Date: Fri, 30 Jun 2000 11:47:39 -0700
Subject: immunofluorescence anti-fade

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X-Mailer: QUALCOMM Windows Eudora Pro Version 4.0


Hi All,
I'm asking this on behalf of a colleague, so please bear with me if I
don't have all of the details.
Does anyone out there have experience with Vectashield as an anti-fade
agent for viewing immunofluorescence? Specifically, we are looking at plant
tissue in which we are using a secondary antibody conjugated to FITC and
have found that the controls (treated only with buffer) show
"autofluorescence" after mounting with Vectashield, when they were totally
non-fluorescent when mounted in buffer. Granted, the Vectashield did expire
last month, but this is something that we've seen before with this product.
Should I advise my colleague to just take pictures as quickly as humanly
possible because we can't seem to rely on this anti-fade agent? I
appreciate any input you may have.
Have a great Independence Day weekend (for those of you in the US),
Kristen
Kristen A. Lennon
Cell, Molecular & Developmental Biology Group
Department of Botany & Plant Sciences
University of California
Riverside, CA 92521
kalen-at-citrus.ucr.edu
909-787-4525



From daemon Fri Jun 30 16:59:34 2000



From: McLean, Dorrance :      dmclea-at-sandia.gov
Date: Fri, 30 Jun 2000 13:21:51 -0600
Subject: Mat. Electropolishing.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} Dear List,
} Does any one have the dummies guide to electropolishing?? Some years ago
} (3), I had a trouble shooting guide for electropolishing. This list had
} about 9 or 10 items, problems /solutions on it and it was very
} helpful...but it has found one of those very safe filing places (i.e. I
} can't find it!) If any one has access to this list or something similar,
} I would appreciate it if you could send me a copy.
}
} Many thanks in advance.
}
} Dorrance
} PS I haven't been keeping up with the weather reports lately but it's 95
} and clear in beautiful Livermore, California.
}



From daemon of you in the US),



From: McLean, Dorrance :      dmclea-at-sandia.gov
Date: Fri, 30 Jun 2000 13:21:51 -0600
Subject: Mat. Electropolishing.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} Dear List,
} Does any one have the dummies guide to electropolishing?? Some years ago
} (3), I had a trouble shooting guide for electropolishing. This list had
} about 9 or 10 items, problems /solutions on it and it was very
} helpful...but it has found one of those very safe filing places (i.e. I
} can't find it!) If any one has access to this list or something similar,
} I would appreciate it if you could send me a copy.
}
} Many thanks in advance.
}
} Dorrance
} PS I haven't been keeping up with the weather reports lately but it's 95
} and clear in beautiful Livermore, California.
}




From: David Henriks :      Henriks-at-CompuServe.COM
Date: Fri, 30 Jun 2000 16:27:32 -0400
Subject: Mat. Electropolishing.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Dorrance:

I do not have a "dummies" guide as such, but I do have copies of Bernie
Kestel's 66 page report "Polishing Methods for Metallic and Ceramic TEM
Specimens". The "polishing" refers to electropolishing. I would be happy
to send this out to you if you do not have it already. I also have dozens
of other papers dealing with electropolishing and hundreds of papers
dealing with specimen preparation. I can email to you a list of the other
papers if you'd like to breeze through that. Let me know.

DISCLAIMER: South Bay Technology produces equipment and supplies for
specimen prepration and, therefore, has a vested interest in promoting
their use.

Best regards-

David
Writing at 1:13:58 PM on 06/30/2000

***************************************************************************
************************

David Henriks
Vice President TEL: 800-728-2233
(toll free in the USA)
South Bay Technology, Inc. +1-949-492-2600
1120 Via Callejon FAX: +1-949-492-1499
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

***************************************************************************
************************

} } } } } Please visit us at http://www.southbaytech.com { { { { {

Manufacturers of precision sample preparation equipment and supplies for
metallography, crystallography and electron microscopy.

Message text written by "McLean, Dorrance"
}
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


} Dear List,
} Does any one have the dummies guide to electropolishing?? Some years ago
} (3), I had a trouble shooting guide for electropolishing. This list had
} about 9 or 10 items, problems /solutions on it and it was very
} helpful...but it has found one of those very safe filing places (i.e. I
} can't find it!) If any one has access to this list or something similar,
} I would appreciate it if you could send me a copy.
}
} Many thanks in advance.
}
} Dorrance
} PS I haven't been keeping up with the weather reports lately but it's 95
} and clear in beautiful Livermore, California.
}

{



From daemon Sat Jul 1 18:03:11 2000



From: Vitaly Feingold :      vitalylazar-at-worldnet.att.net
Date: Thursday, June 29, 2000 7:51 PM
Subject: SEM: LaB6-to-anode distance

Contents Retrieved from Microscopy Listserver Archives
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Michael,

You can find the information about LaB6 emitter setting and operation in
Kimball Physics technical bulletins. I am aware of 7 bulletins being
available. Contact Kimball Physics at info-at-kimphys.com . Bulletins are
not posted, so you will have to request them. Ask for at least 2: bulletin #
LaB6-01B (General guidelines for operating ES-423E LaB6 cathodes) and
bulletin # LaB6-03B (Emission drift - LaB6 and gun stability). Kimball
Physics guidelines for LaB6 emitter use are comprehensive yet well suited
for practical applications.

You are welcome to contact me off line for the fax copy, though I do not
have the complete set of bulletins.

Disclaimer: SIA does not have an affiliation with Kimball Physics other than
being satisfied customer.

Vitaly Feingold
Scientific Instruments and Applications
2773 Heath Lane, Duluth GA 30096
(770)232-7785 ph.
(770)232-1791 fax
-----Original Message-----
} From: =shAf= {mshaf-at-darkwing.uoregon.edu}
To: Microscopy list {Microscopy-at-sparc5.microscopy.com}




From daemon Sun Jul 2 04:30:31 2000



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Sun, 2 Jul 2000 21:14:05 GMT+1200
Subject: still on about the OL

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No replies to my posting!

Don't you guys ever check this flow?
What if the needle valve or (on the 840A) the solenoid shutoff valve
gets blocked?
Come on, someone must have done it!

Incidentally, when I pulled apart the solenoid shutoff valve on my
840A, a small amount of corrosion had jammed it permanently "OPEN".
This is potentially dangerous, as if the water continues to circulate
thru the OL when the instrument is switched off, and if the cooling
water is below the dewpoint, condensation on the outside of the OL
can wreck it by corrosion.

So if you do decide to check the valve on yours, how about measuring
the flow for me?

TIA

rtch




} ------------------------------------------------------------------------
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}
}
} Hi
}
} Can anyone advise what the water flows should be in the three
} parallel branches (OL; DPs; and power boards) of the 840A)
} The manual spec is } 5 l/min total, but I need to know particularly
} what the flow should be thru the OL.
}
} TIA
}
} rtch
}
} ps replies welcome from JEOL
}

Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand



From daemon Sun Jul 2 07:32:27 2000



From: jim :      jim-at-proscitech.com.au
Date: Sun, 2 Jul 2000 17:11:46 +1000
Subject: RE: Procedure for handling LN2

Contents Retrieved from Microscopy Listserver Archives
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I completely agree with the comments by Warren Straszheim and Larry Stoter. A
couple more points:
1 Gasoline is rather more dangerous then liquid nitrogen. If anybody would try
to impose silly forms and rules and thus curtailing the publics access to
gasoline, they be out on their ear fast. Access to gas is available to
countless completely untrained people.
2 What evidence is there that serious accidents have occurred by small scale
handling of liquid nitrogen. Golden rule of administration: Regulate only when
there is a demonstrated need.
3 Its my observation that timid people (intimidated by overstated dangers) are
more likely to cause accidents. I instructed first time users of liquid
nitrogen that there is a danger when the substance is enclosed, that touching
cold metal or pouring it into ones shoes causes burns and that cold tubing can
fracture.
As important, new users must learn that its quite harmless when a little is
splashed and its worth demonstrating that a finger can be stuck into liquid
nitrogen for a couple of seconds. This builds handling confidence.

The safety officer's paper wastes time and paper. Users need to learn how to
handle the substance; its simple enough. Writing up a procedure for pumping
creates a delusion, which has no practical benefits.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Saturday, July 01, 2000 3:39 AM, Warren E Straszheim
[SMTP:wesaia-at-iastate.edu] wrote:
}
}
} Hi, Everett. I guess I am glad I am not working for a government anymore.
} Otherwise I would probably be having to fill out similar forms. It would be
} nice if the folks that review those forms knew what the issues were. I am
} always afraid that they will know just enough to be dangerous and may
} actually require some unhealthy procedures. But perhaps they are sensible
} and only require that you have something officially in writing to document
} your best practice.
}
} I found the following post in my files from 4 years ago. I hope Larry does
} not mind me reposting it. His conclusions are quite interesting.
}
} Warren S.
}
}
} Date: Sat, 30 Mar 1996 07:57:02 +0000
} } From: "Dr. L. P. Stoter" {LPS-at-teknesis.demon.co.uk}
}
} } Wil's recent comment on the safety hazards of distilled water brought to
} } mind some peculiar safety regulations here in MD. In reference to liquid N
} ...
} } officer listening in will recommend new safety procedures requiring
} } protective booties!
} } In the end, we can't legislate common sense, nor can we abdicate
} } responsibility to those above.
}
} Try getting your safety officer to conduct an experiment:
}
} 1. Hold out hand,
} 2. Pour a small volume of liquid N2 over hand
} 3. Now the interesting bit - put on a glove, and pour the same quantity of
} liquid N2 into glove.
} 4. Phone for ambulance.
}
} The point is that a brief contact causes no problems, but if the contact is
} continued you get a nasty burn.
}
} Gloves, goggles, masks (and shoes) are actually more dangerous when handling
} liquid N2 than sandals and no protection. And clothes are actually more
} dangerous than being naked. Get the safety officer to experiment. With a
} little persuasion you can probably convince the safety officer that when
} handling liquid N2, everybody should be naked.
}
} More seriously, bureaucrats, administrators and the inexperienced should
} talk to somebody who has real knowledge.
}
} --------------------------------------------------------------
} Dr. Larry Stoter
} Technesis
} 17, Rocks Park Road, Uckfield, E. Sussex, TN22 2AT, United Kingdom
} Larry-at-teknesis.demon.co.uk
} --------------------------------------------------------------
}




From daemon Sun Jul 2 08:52:20 2000



From: Pbgrover-at-aol.com
Date: Sun, 2 Jul 2000 13:07:17 EDT
Subject: re: handling LN2

Contents Retrieved from Microscopy Listserver Archives
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In SEMs the objective lens current required to "bend" the beam is much less
than in TEM where the uncooled objective could fry an egg. Many SEMs use no
water cooling at all. Cooling would help temperature stability and control
drift. I expect that 50ml /minute would do, but such slow flow would soon stop
altogether. I suggest that less than 2 liters/ minute indicate blocked tubing.
Reverse flushing my help. Otherwise recirculate hot vinegar or 1N HCl for an
hour.
Cross threading: I never had a safety officer, get him to do it, otherwise you
may require a ream of paper applications for pumping acid.
Happy now Rich?
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

-----Original Message-----
} From: Ritchie Sims [SMTP:r.sims-at-auckland.ac.nz]
Sent: Monday, July 03, 2000 7:14 AM
To: Ritchie Sims; Microscopy-at-sparc5.microscopy.com


Dr. Larry Stoter (4 years ago) wrote: (and thanks for sharing it, Jim)

Gloves, goggles, masks (and shoes) are actually more dangerous when handling
} liquid N2 than sandals and no protection. And clothes are actually more
} dangerous than being naked. Get the safety officer to experiment. With a
} little persuasion you can probably convince the safety officer that when
} handling liquid N2, everybody should be naked.

I'd like to share my experience with getting naked in a hurry. I was
working around midnight in the biochem lab at Okla. State Univ. in '79 and
was freezing samples to put in the lyophilizer. My arms were full, carrying
numerous flasks plus a dewar of LN2, which I held against my shoulder. As I
turned a corner, I slipped on some spilled water (I was wearing those flip
flop sandals) and the entire contents of the dewar poured down my back.
Fortunately there were no witnesses to my rapid striptease. (I suffered only
a minor burn on my upper back.) I heartily agree with Larry that nudity is
the way to go if you plan to spill LN2 on yourself!

Cheers :o)

Paul Grover
Chief Microscopist and Bottle Washer
Microvista Laboratory
Lafayette, IN



From daemon Sun Jul 2 16:30:08 2000



From: Larry Stoter :      LPS-at-teknesis.demon.co.uk
Date: Sun, 2 Jul 2000 22:12:01 +0100
Subject: RE: Procedure for handling LN2

Contents Retrieved from Microscopy Listserver Archives
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} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Actually, not my comments, although it is a thread to which I
contributed and generally reflects my sentiments.

Regards,
--
Larry Stoter
34, Astwick Road, Stotfold, Hitchin, Herts, SG5 4AU, UK
email: LPS-at-teknesis.demon.co.uk, Home Phone/Fax: +44 (0)1462 733309



From daemon Sun Jul 2 18:12:15 2000



From: Victor Sidorenko :      antron-at-space.ru
Date: Sun, 2 Jul 2000 01:29:14 +0400
Subject: Re: JEOL 840A cooling water flow

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Hi Ritchie
As I remember the relevant cock should be open on 1/4 turnover. It is
necessary to check, that the water on exit is not very warm at max
current of objective lens. On the other hand, I know the case, when
the water was very cold, therefore the condensate accumulated in the
lens. I figure, the consequences are clear to you.
Regards
Victor Sidorenko, ANTRON Co. Ltd., Moscow, Russia


-----Èñõîäíîå ñîîáùåíèå-----
Îò: Ritchie Sims {r.sims-at-auckland.ac.nz}
Êîìó: Microscopy-at-sparc5.microscopy.com {}
Äàòà: 29 èþíÿ 2000 ã. 10:00
Òåìà: JEOL 840A cooling water flow


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Hi

Can anyone advise what the water flows should be in the three
parallel branches (OL; DPs; and power boards) of the 840A)
The manual spec is } 5 l/min total, but I need to know particularly
what the flow should be thru the OL.

TIA

rtch

ps replies welcome from JEOL


Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand





From daemon Sun Jul 2 21:04:36 2000



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Mon, 3 Jul 2000 13:46:01 GMT+1200
Subject: LN2 not quite so safe

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} As important, new users must learn that its quite harmless when a little is
} splashed and its worth demonstrating that a finger can be stuck into liquid
} nitrogen for a couple of seconds. This builds handling confidence.


I think that this is going a bit far.

Ever had any splashed into your eye? Only needs a drop in the wrong
place.

cheers

rtch


Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand



From daemon Sun Jul 2 22:36:58 2000



From: Earl Weltmer :      earlw-at-pacbell.net
Date: Sun, 02 Jul 2000 20:20:32 -0700
Subject: Re: handling LN2

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Hi All,

I have been reading with interest about the handling of LN2 and am
surprised that
no one has taken the safety officer's conservative position.

I am all for getting bureaucrats out of our hair but not at the expense
of
safety.
I agree the the extra clothing, goggles and what have you are cumbersome
and most
of the time un-necessary but I am only reminded of an incident that
happened to
me about 25 years ago.

I was routinely filling a portable LN2 tank from a larger source. The
portable
tank had a pressure gauge that would measure tank pressure: when full
the gauge
read 20 psi; empty 0 psi. The assembly was attached to the tank via a
rubber
vacuum hose clamped at each end.

The normal procedure was to release the "vent" valve, unclamp the rubber
hose,
remove the valve assembly, then refill the portable tank from the larger
LN2
source. I am sure most of you have seen a similar assembly.

Early one morning (before my coffee), I went through the above
procedure. After
unclamping the rubber hose, I proceeded to remove the assembly from the
tank
using both hands to pry it loose.
Unfortunately there was a little pressure left and as soon as the hose
was
released, a stream of LN2
burst from the tank. The noise startled me and in less than a half
second I
removed my hands from the tank. In less than one second, I realized I
was burned
and immediately immersed both hands into water from a nearby sink. Too
late. In
less than one-half second, I received second and third degree LN2 burns
on the
bottom of my hands. The blisters extended from the bottom of both hands
to
halfway up my small finger. Worse yet, both hands were bleeding
.Apparently, both
hands immediately froze and I had cracked the skin by moving them to the
sink.

I wish I had gloves at that time.

I worried for the next two weeks about getting gangrene.

Still today, I don't use gloves as they are cumbersome but I would hate
to think
what would happen if I were sprayed in the eyes from LN2 even for a
split second.

Regards,

Earl Weltmer

"Pbgrover-at-aol.com"-at-sparc5.microscopy.com wrote:

}
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America
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-----------------------------------------------------------------------.

}
} Dr. Larry Stoter (4 years ago) wrote: (and thanks for sharing it,
Jim)
}
} Gloves, goggles, masks (and shoes) are actually more dangerous when
handling
} } liquid N2 than sandals and no protection. And clothes are actually
more
} } dangerous than being naked. Get the safety officer to experiment.
With a
} } little persuasion you can probably convince the safety officer that
when
} } handling liquid N2, everybody should be naked.
}
} I'd like to share my experience with getting naked in a hurry. I
was
} working around midnight in the biochem lab at Okla. State Univ. in '79
and
} was freezing samples to put in the lyophilizer. My arms were full,
carrying
} numerous flasks plus a dewar of LN2, which I held against my
shoulder. As I
} turned a corner, I slipped on some spilled water (I was wearing those
flip
} flop sandals) and the entire contents of the dewar poured down my
back.
} Fortunately there were no witnesses to my rapid striptease. (I
suffered only
} a minor burn on my upper back.) I heartily agree with Larry that
nudity is
} the way to go if you plan to spill LN2 on yourself!
}
} Cheers :o)
}
} Paul Grover
} Chief Microscopist and Bottle Washer
} Microvista Laboratory
} Lafayette, IN




From daemon Sun Jul 2 23:05:59 2000



From: jim :      jim-at-proscitech.com.au
Date: Mon, 3 Jul 2000 13:55:07 +1000
Subject: RE: LN2 not quite so safe

Contents Retrieved from Microscopy Listserver Archives
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Why? A droplet of liquid N2 would bounce of the eye just like of any other skin
and the cushion of gas N2 (Leidenfrost) would help to prevent any damage.
I don't suggest to pour liq N2 into an eye and its prudent to wear goggles when
working with the stuff overhead, but why would you work with liq N2 high up on
your body? Surely when filling an EDS detector on a TEM (higher up than SEM)
you would use steps.
Cheers
Jim Darley

ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Monday, July 03, 2000 11:46 PM, Ritchie Sims [SMTP:r.sims-at-auckland.ac.nz]
wrote:
}
} } As important, new users must learn that its quite harmless when a little is
} }
} } splashed and its worth demonstrating that a finger can be stuck into liquid
} }
} } nitrogen for a couple of seconds. This builds handling confidence.
}
}
} I think that this is going a bit far.
}
} Ever had any splashed into your eye? Only needs a drop in the wrong
} place.
}
} cheers
}
} rtch
}
}
} Ritchie Sims Phone : 64 9 3737599 ext 7713
} Department of Geology Fax : 64 9 3737435
} The University of Auckland email : r.sims-at-auckland.ac.nz
} Private Bag 92019
} Auckland
} New Zealand



From daemon Sun Jul 2 23:38:32 2000



From: Allen R. Sampson :      ars-at-sem.com
Date: Sun, 2 Jul 2000 23:24:10 -0500
Subject: RE: Procedure for handling LN2

Contents Retrieved from Microscopy Listserver Archives
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Normally, it is easier, more efficient and safer to use a small ( {10L)
transfer dewar to actually fill an EDS dewar. LN2 itself, as several
others have pointed out, is quite safe to handle. The nudity
recommendations are only slightly tongue in cheek. Small quantities of LN2
on the skin will 'float' on a vapor phase that forms immediately, avoiding
close contact. If the LN2 is able to wedge between skin and gloves or
other clothing, a more intimate and hazardous situation can occur.

The real problem with the use of cryogenic hoses is that the exterior
metals can also achieve cryogenic temperatures. While they will generally
get coated with ice, the underlying metal can burn if touched. When the
contents of a dewar are first being drawn, the warm hoses will cause the
fluid to evaporate. For the first minute or two, all that will be going
into the EDS dewar will be cold vapor. That could cause an accumulation of
ice in the EDS dewar that can affect performance.

The use of a small transfer dewar allows you to only put liquid LN2 in the
EDS dewar. Also, you have a more immediate control of the flow into the
dewar. One other problem using a hose feed system is that you may turn the
pressure up too far at first, since there is only vapor passing through.
When the fluid starts flowing, the pressure may be high enough to cause
excessive 'splashing'.


Allen R. Sampson, Owner
Advanced Research Systems, St. Charles, Illinois 60174
voice 630.513.7093 fax 630.513.7092

On Friday, June 30, 2000 6:35 AM, Everett Ramer
[SMTP:Everett.Ramer-at-netl.doe.gov] wrote:
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}
}
} I will getting my first SEM/EDS shortly and to obtain the required
operating permit from my safety group I need a written procedure for
handling LN2. Specifically I need a written procedure for filling the 3 L
dewar on the EDS detector from a 50 L dewar mounted on a cart. I plan to
make the transfer by using a lab source of N2 to pressurize the 50 L dewar
and have obtained all the valves and fittings required to do this from
another SEM lab. I would appreciate copies of the procedure.
} Thanks,
}
} Everett Ramer
} National Energy Technology Laboratory
} P.O. Box 10940, Cochrans Mill Road
} Pittsburgh, PA, USA 15236-0940
} Voice: 412-386-4920
} FAX: 412-386-4806
} ramer-at-netl.doe.gov
}
}
}



From daemon Mon Jul 3 01:36:49 2000



From: Cheng Huang :      HUANG-at-rsbs.anu.edu.au
Date: Mon, 03 Jul 2000 16:23:05 +1000
Subject: Used Oxford Cryo-stage wanted

Contents Retrieved from Microscopy Listserver Archives
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We are looking for an used Oxford cryo-stage, prep-chamber and control
panels for our Jeol 6400 SEM. If anybody wants to sell their old one, please
let us know.

Cheng H.

Microscopy centre
CSIRO
Australia



From daemon Mon Jul 3 04:45:03 2000



From: Dr Eric Lachowski :      che136-at-abdn.ac.uk
Date: Mon, 3 Jul 2000 10:33:38 +0100 (BST)
Subject: liquid nitrogen

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I am a microscopist who also has the misfortune to be the
Departmental Safety Adviser and I have a few comments to
make from the point of view of the enemy. While the jokey
remarks about the advisability of wearing clothing,
protective or otherwise, do have an element of truth,
liquid nitrogen has the potential to do a great deal of
harm. Last month there was a prosecution in Edinburgh
where the Medical Research Council was found guilty of
breaches of Health and Safety law after a technician died
of asphyxiation while dispensing LN2 in a room with
inadequate ventilation and three of his colleagues came
close to suffering the same fate while trying to rescue
him. The room was fitted with a low oxygen alarm but it was
switched off because it went off too frequently and annoyed
him. This is an example of familiarity leading to
dangerous practices. (His bosses got hammered because they
knew about the alarm being switched off.)

To write a procedure you need to think of what could go
wrong, as well as the routine safe handling of the LN2,
which is basically covered by - would you believe it
-common sense.
What would happen if the dewar shattered? A full shield
should stop LN2 fron getting up your nose or in your mouth
and is better than goggles which might trap liquid insude
them. Drain holes in the bottom of the outer case of
the dewar will reduce the risk of your hand freezing to it.
What would happen if the main tank ruptured or fell over?
I know that this is very unlikely, but a simple calculation
based on the volume of the room and the capacity of the
tank will tell you if oxygen depletion to a dangerous level
is a significant possibility - and it will look good on
your paperwork.

LN2 burns are particularly nasty as you may not be aware of
them until the frozen bit thaws out again, by which time
it may be too late to prevent serious damage so you might
want to consider gloves if you are to handle cold parts of
the apparatus. These must be proper cryo-gloves, but it
has to be said that they offer limited protection and can
in certain circumstances be worse than not wearing gloves
at all.

Remember that if it can happen it will,

Eric
----------------------
Dr Eric Lachowski
Department of Chemistry
University of Aberdeen
Meston Walk
Old Aberdeen AB24 3UE
Scotland
+44 (0)1224 272934 fax 272921
e.lachowski-at-abdn.ac.uk






From daemon Mon Jul 3 04:46:09 2000



From: Malcolm Haswell :      malcolm.haswell-at-sunderland.ac.uk
Date: Mon, 03 Jul 2000 10:29:46 +0100
Subject: Re: Procedure for handling LN2

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Dear all

I can't really leave this one alone - it's just begging for a contrary opinion.

The point about gloves holding liquid nitrogen is of course true if they either fill
from the wrist or are porous, but you should never wear tight gloves - it should
always be possible to shake or flick them off (ideally without leaving fingers
inside) and it is possible to get quite impervious gloves. I am extremely concerned
about the rejection of need for protection because it could too easily mean that you
didn't have any gloves handy when surfaces became cooled. More importantly if you
handle super-cooled liquid nitrogen (or countless other cooling mixtures) for
freezing samples then you just might forget. I am also interested in the comments
about goggles and masks being more dangerous as my understanding was that liquid
nitrogen is relatively safe on dry surfaces but presents a greater hazard to the wet
surface of the eye - if only by temporary blinding the user or causing them to
flinch.

You might also take for granted the nitrogen gas evolving and the consequences could
be fatal in an enclosed space - see:

http://www.safetynews.co.uk/archivenews.htm#£25,000 fine for Human Genetics Unit
liquid nitrogen fatality

I must admit that apart from the above I have seen little, but I would be interested
to hear how a nudist colony would really fare with a major liquid nitrogen leak -
remember the horror stories about frying sausages in nudist camps.

I would also like to counter the thought that gasoline is more dangerous than liquid
nitrogen. It's different - I carry a 4.5 litre sealed container of gasoline/petrol
around in my car quite safely but I wouldn't seal liquid nitrogen like that; open or
poorly sealed storage can lead to icing or condensation of oxygen from air (now
there's a good one to debate - has anyone heard of that causing an accident?) with
possible disastrous consequences; surfaces in prolonged contact with gasoline would
present less danger than with liquid nitrogen; and gasoline has a strong odour so I
wouldn't expect to be asphyxiated by it so easily (OK it might ignite more easily
and be harmful).

Now I must go because I'm on the third page of my triple distilled water risk
assessment.

Malcolm Haswell
University of Sunderland
UK
e-mail: malcolm.haswell-at-sunderland.ac.uk


Larry Stoter wrote:

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} }
} }
} } I completely agree with the comments by Warren Straszheim and Larry Stoter. A
} } couple more points:
} } 1 Gasoline is rather more dangerous then liquid nitrogen. If
} } anybody would try
} } to impose silly forms and rules and thus curtailing the publics access to
} } gasoline, they be out on their ear fast. Access to gas is available to
} } countless completely untrained people.
} } 2 What evidence is there that serious accidents have occurred
} } by small scale
} } handling of liquid nitrogen. Golden rule of administration: Regulate only when
} } there is a demonstrated need.
} } 3 Its my observation that timid people (intimidated by
} } overstated dangers) are
} } more likely to cause accidents. I instructed first time users of liquid
} } nitrogen that there is a danger when the substance is enclosed, that touching
} } cold metal or pouring it into ones shoes causes burns and that cold tubing can
} } fracture.
} } As important, new users must learn that its quite harmless when a little is
} } splashed and its worth demonstrating that a finger can be stuck into liquid
} } nitrogen for a couple of seconds. This builds handling confidence.
} }
} } The safety officer's paper wastes time and paper. Users need to learn how to
} } handle the substance; its simple enough. Writing up a procedure for pumping
} } creates a delusion, which has no practical benefits.
} } Cheers
} } Jim Darley
} } ProSciTech Microscopy PLUS
} } PO Box 111, Thuringowa QLD 4817 Australia
} } Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
} } Great microscopy catalogue, 500 Links, MSDS, User Notes
} } ABN: 99 724 136 560 www.proscitech.com
} }
} } On Saturday, July 01, 2000 3:39 AM, Warren E Straszheim
} } [SMTP:wesaia-at-iastate.edu] wrote:
} } }
} } }
} } } Hi, Everett. I guess I am glad I am not working for a government anymore.
} } } Otherwise I would probably be having to fill out similar forms. It would be
} } } nice if the folks that review those forms knew what the issues were. I am
} } } always afraid that they will know just enough to be dangerous and may
} } } actually require some unhealthy procedures. But perhaps they are sensible
} } } and only require that you have something officially in writing to document
} } } your best practice.
} } }
} } } I found the following post in my files from 4 years ago. I hope Larry does
} } } not mind me reposting it. His conclusions are quite interesting.
} } }
} } } Warren S.
} } }
} } }
} } } Date: Sat, 30 Mar 1996 07:57:02 +0000
} } } } From: "Dr. L. P. Stoter" {LPS-at-teknesis.demon.co.uk}
} } }
} } } } Wil's recent comment on the safety hazards of distilled water brought to
} } } } mind some peculiar safety regulations here in MD. In reference
} } } to liquid N
} } } ...
} } } } officer listening in will recommend new safety procedures requiring
} } } } protective booties!
} } } } In the end, we can't legislate common sense, nor can we abdicate
} } } } responsibility to those above.
} } }
} } } Try getting your safety officer to conduct an experiment:
} } }
} } } 1. Hold out hand,
} } } 2. Pour a small volume of liquid N2 over hand
} } } 3. Now the interesting bit - put on a glove, and pour the same quantity of
} } } liquid N2 into glove.
} } } 4. Phone for ambulance.
} } }
} } } The point is that a brief contact causes no problems, but if the contact is
} } } continued you get a nasty burn.
} } }
} } } Gloves, goggles, masks (and shoes) are actually more dangerous when handling
} } } liquid N2 than sandals and no protection. And clothes are actually more
} } } dangerous than being naked. Get the safety officer to experiment. With a
} } } little persuasion you can probably convince the safety officer that when
} } } handling liquid N2, everybody should be naked.
} } }
} } } More seriously, bureaucrats, administrators and the inexperienced should
} } } talk to somebody who has real knowledge.
} } }
} } } --------------------------------------------------------------
} } } Dr. Larry Stoter
} } } Technesis
} } } 17, Rocks Park Road, Uckfield, E. Sussex, TN22 2AT, United Kingdom
} } } Larry-at-teknesis.demon.co.uk
} } } --------------------------------------------------------------
} } }
}
} Actually, not my comments, although it is a thread to which I
} contributed and generally reflects my sentiments.
}
} Regards,
} --
} Larry Stoter
} 34, Astwick Road, Stotfold, Hitchin, Herts, SG5 4AU, UK
} email: LPS-at-teknesis.demon.co.uk, Home Phone/Fax: +44 (0)1462 733309




From daemon Mon Jul 3 06:10:49 2000



From: Mary Gail Engle :      mgengle-at-pop.uky.edu
Date: Mon, 03 Jul 2000 09:00:09 -0400
Subject: Epon 812

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Becky,

as a manufacturer of large chamber SEMs with EDX it would be a pleasure for
us to analyze your sample. The max. size of your sample should not exceed 28
inches in diameter and 24 inches in height.

To give you an impression about the technology of large chamber SEMs just
click it http://www.visitec-em.de/tokyo.html

Greetings from Germany
Martin Klein

----------------------------------------------------------------------------
------
VisiTec Microtechnik GmbH
Karl-Marx-Str. 14
D-23936 Grevesmuehlen/Germany
Fon: +49-3881-79049
Fax: +49-3881-79048

email: mklein-at-visitec-em.de
WWW: http://www.visitec-em.de

----- Original Message -----
} From: Becky Holdford {r-holdford-at-ti.com}
To: Microscopy ListServer {microscopy-at-sparc5.microscopy.com}
Sent: Saturday, July 01, 2000 2:54 AM


Hi All,
there is a serious side to LN2 handling even though many points
discussed are valid. I have found most problems associated with LN2 are
caused by things other than the LN2 itself. I remember when I first started
using LN2 over 25 years ago I was filling a glass vacuum flask when it
exploded and filled the room I was in with instant fog, tinsel and plenty of
LN2 as a fine spray. I was unharmed apart from fine glass shards and Al foil
in my hair. I was wearing no protective gear. The LN2 had also been in my
hair as a fine spray thank goodness. I was amazed that I had no effect from
the LN2. After that I started to wear thermal type gloves but soon found
that these were more dangerous than no gloves at all.
There are times when one does have to be careful in handling LN2 and these
in my experience have been with 1/ glass containers. 2/ pressurised
containers and 3/Accidental contact from surfaces at very cold temperatures.

I am sure that there have also been other experiences that we can learn from
so that we can understand how we can avoid them.

Ken Moran.

Moran Scientific Pty Ltd
P.O. Box 651
Goulburn NSW 2580 Australia
Tel 02 4844 4234 (International, 61 2 48444234)
Fax 02 4844 4291 (International, 61 2 48444291)
Email {kmoran-at-goulburn.net.au}
Web Page http://www.goulburn.net.au/~kmoran/
"Patience accomplishes its object, while hurry speeds to its ruin. Gulistan
1258"



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Leona,
I also have bottles of Epon 812 in my lab, some of which have been opened,
and all of which are from the early 1970's. I still use it for certain
applications when nothing else works. Epon 812 blocks will pop off some
plastic culture wells when nothing else will. Even though the dishes are
made of the same material there is some variation from brand to brand and
when in doubt I use the old resin. I haven't varied the original recipe
and have had no problems. I'll buy it from you if you decide not to keep it.

Mary Gail Engle
Electron Microscopy & Imaging Facility
University of Kentucky



From daemon Mon Jul 3 08:48:50 2000



From: Claudia Hayward-Costa :      LS_S562-at-crystal.kingston.ac.uk
Date: Mon, 3 Jul 2000 14:40:39 +0100
Subject: SEM: Problems with retaining cells on filter

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Microscopists,

I was searching through the archives - found numerous helpful
suggestions, however no real answer to my recent problem.

Here it is:
We were investigating cells in suspension and blood cells in the
SEM. Very straight forward - after fixation in suspension the cells
were filtered on to polycarbonate filters (0.4 um) with a 1ml syringe.

At this stage I removed the filters from the holder and put them into
multiwell plates for additional washes with buffer and dehydration in
alcohol. The multiwell plate was placed on a gently shaking rotator.
The cells on the filter were dried via liquid substitution, gold coated
and observed.

Although the cells survived the procedure structurally quite well I
was surprised that we had to hunt a lot to actually find the cells on
the filter. The pore size of the filter is too small for human blood
cells to escape through - were has 90% of the population gone?

Would it be better to place unfixed cells on the filter?

Is there a way to make them adhere better?

Any suggestion is appreciated - this list is a fantastic source of
know-how.

Thank you.

Claudia


Dr. C. Hayward-Costa
School of Life Sciences
Kingston University
Penrhyn Road, Kingston upon Thames
Surrey KT1 2EE, UK
44(0)208 547 2000 x 2240
Email: c.hayward-at-kingston.ac.uk



From daemon Mon Jul 3 09:40:59 2000



From: Geoff Williams :      willi1gl-at-cmich.edu
Date: Mon, 03 Jul 2000 17:44:35 -0400
Subject: AMRAY 1200 - Anyone need one?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,
I use Epofix epoxy. In a very short time after purchase the resin becomes cloudy and a white sediment forms at the bottom of the bottle. I have heard this sediment described as crystals. I have continued to use the resin---carefully avoiding agitation and entrainment of the sediment when I withdraw it from the bottle. Does anyone else have experience with this?

Everett Ramer
National Energy Technology Laboratory
P.O. Box 10940, Cochrans Mill Road
Pittsburgh, PA, USA 15236-0940
Voice: 412-386-4920
FAX: 412-386-4806
ramer-at-netl.doe.gov



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I would require a used / 2nd hand Cambridge S360 IP backplane.Could anybody
help me out ?

Thanks,
Neville Baker

Anaspec, South Africa
Technical support on microscopy.
Tel + 27 (0) 11 476 3455
Fax + 27 (0) 11 476 7290
anaspec-at-icon.co.za
www.anaspec.co.za

see you at ICEM 15
2002, Durban, South Africa
www.icem15.com




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Colleagues,

We are looking into the possibility of mating a Zeiss AttoArc fluorescence
lamphouse (for HBO100 bulb) to a Leica DMIRB inverted research microscope.
Has anyone had experience doing such a thing?

We can certainly manufacture an adapter to mount the lamphouse to the Leica
scope; that's a minor problem. But I am more concerned about the length of
the beam path and whether the collector lens in the Atto source will bring
the beam to the proper focus point.

I should mention that we have the Leica scope but we haven't yet found an
Atto lamphouse and power supply. We have tried to contact Atto but so far we
have not gotten a response. Perhaps the Atto rig is sold exclusively for
Zeiss scopes? I have done some initial measurements on a Zeiss inverted
scope that has an AttoArc lamphouse on it, and I do believe that it would
work just fine. But some confirmation from another source would be
appreciated!

If anyone has done this before, I would certainly like to hear from you.
Please contact me off-list to discuss.

Thanks for your input.

Cheers,

Bob Chiovetti
rchiovetti-at-aol.com


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Greetings,

We are currently planning to teach the introductory undergrad SEM course
on our JEOL 840A with the digital output. In doing so we are dropping
the 4 month/year service contract and most likely we are going to
discontinue use of the AMRAY 1200.

I am trying to get a feel of the market for an old (early 1970s)
solid-state SEM. The imaging is fantastic - really quite amazing. But
there are quite a few limitations - resolution, magnification, only film
output (4X5 Polaroid or Sheet film), scan speeds.

Is this a machine that anyone might be able to make use of? One idea I
had was this little table top wonder machine would make a great personal
SEM, although for me personally why take it home when I can use the 840A
when ever I want.

Again, I am not committing this good old 'scope to the open market or
the open scrap heap (the other possible avenue for this scope is to call
the scrap yard to haul it off) yet, I just need some potential options
to through at the Chairman and the Dean.

Any third party folks that are servicing these have a desire for
some/all of it?

Geoff

--
Geoff Williams

Electron Microscope Facility Supervisor
Biology Department
Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859

Geoffrey.Lloyd.Williams-at-cmich.edu
517 774-3576
517 774-3462 (fax)





From daemon Mon Jul 3 20:44:23 2000



From: Walck, Scott D. :      walck-at-ppg.com
Date: Mon, 3 Jul 2000 21:28:41 -0400
Subject: used Kreonite Darkroom processor to a good home

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have a Kreonite automatic photographic paper processor that will go to
the scrap heap very soon if no one wants it. You would probably have to
arrange for shipping. If anyone would like to have it (and a few spare
parts), it can be arranged. Preference will go to a university, but we are
interested in getting it out of the storage area. It was working when we
stored it. It comes with a cart that was made for it. If you process a lot
of paper, this is the unit for you. Let me know this week.


-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)





From daemon Mon Jul 3 21:27:25 2000



From: ard-at-ansto.gov.au (Arthur Day)
Date: Tue, 4 Jul 2000 12:17:25 +1000
Subject: Re: Procedure for handling LN2

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} I would also like to counter the thought that gasoline is more dangerous
} than liquid
} nitrogen. It's different - I carry a 4.5 litre sealed container of
} gasoline/petrol
} around in my car quite safely but I wouldn't seal liquid nitrogen like
} that; open or
} poorly sealed storage can lead to icing or condensation of oxygen from air (now
} there's a good one to debate - has anyone heard of that causing an
} accident?) with
} possible disastrous consequences; surfaces in prolonged contact with
} gasoline would
} present less danger than with liquid nitrogen; and gasoline has a strong
} odour so I
} wouldn't expect to be asphyxiated by it so easily (OK it might ignite more
} easily
} and be harmful).
}

Now there's a thought. To digress a little, we once had an instance where a
guy came down to the SEM lab and asked if he could borrow a 5 litre dewar
to take some LN2 home and show his kids. When asked for more details his
game plan was to sit the dewar on the floor on the passenger side of the
car so that he could make sure that it wouldn't fall over. The answer was
"No, perhaps you should bring the kids to the LN2...."

The point being that even though LN2 use might seem less of a risk than
boiling water when used "properly" and "common sense" is applied, it cannot
be assumed without question that everyone's initial mental picture behind
these two terms is exactly the same!!


Arthur Day, Electron Microscope Unit Phone: 61-2-9717-3457
Ansto Materials Division Fax: 61-2-9543-7179
PMB 1, Menai (Sydney), NSW, 2234 Email: ard-at-ansto.gov.au
Australia www: http://www.ansto.gov.au/





From daemon Tue Jul 4 08:35:28 2000



From: HARRISm-at-esm-semi.co.uk
Date: Tue, 04 Jul 2000 14:16 +0000 (GMT)
Subject: An amusing N2 STORY

Contents Retrieved from Microscopy Listserver Archives
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Nitrogen Dangerous ?

An incident I witnessed :

We were having a coffee break in a portacabin room used as a makeshift
lab when a delivery lorry about to top up our cryogenic liquid N2 tank
reversed into and broke the connecting refill valve . We were
observing the resulting stream of liquid N2 spill over the tarmac when
one of the guys remembered that his new car was parked in the enclosed
area and his new tyres may be damaged .
Without a word he pushed through the firedoor , ran the few yards
through the increasingly heavy fog and managed to reverse his car in
the few feet available out of the way of the liquid nitrogen .
But when he went to return he found the fog too heavy to see the few
yards to the portacabin , and quickly became disorientated . As panic
set in he fell to his knees and ,becoming increasingly short of
breath, started crawling- finally to the firedoor which by now was
firmly closed as we had all run for safety .
Fearing asphyxiation he managed to crawl around the building through
the fog and emerged gasping a minute or so later .
The contrast to one minute quietly having a coffee and the next to
witness this life and death ? struggle and accusations of attempted
murder as someone had closed the firedoor caused much merriment at the
time .
All except for the poor 'victim' ( sorry Leighton if your reading
this ) .

Martyn




From daemon Wed Jul 5 21:56:38 2000



From: Chuck Butterick :      cbutte-at-ameripol.com
Date: 7/3/00 10:28 PM
Subject: LN2 Spills

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I've avoided commenting on LN2 to this point, because one person became
quite offended at my comments the 4-5 years ago when this thread was
explored in a very similar manner. Ken's comments about shattered thermos
is an experience, though, I've shared too many times.

Besides being the glass thermos being a different kind of hazard after
breaking, a 5L dewar is very expensive. Just over a year ago I started
using a 2 gallon plastic drink dispenser from WalMart. Cost less than $10.
It lasted almost a year before the seal around the spigot failed. Of
course you can't store LN2 for any length of time but for transport between
tank and EDX detector, it does great. BTW, the top vent was permanently
removed so no pressure buildup could occur.

Chuck Butterick
Engineered Carbons, Inc.
Borger, TX

______________________________ Reply Separator _________________________________


------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Hi All,
there is a serious side to LN2 handling even though many points
discussed are valid. I have found most problems associated with LN2 are
caused by things other than the LN2 itself. I remember when I first started
using LN2 over 25 years ago I was filling a glass vacuum flask when it
exploded and filled the room I was in with instant fog, tinsel and plenty of
LN2 as a fine spray. I was unharmed apart from fine glass shards and Al foil
in my hair. I was wearing no protective gear. The LN2 had also been in my
hair as a fine spray thank goodness. I was amazed that I had no effect from
the LN2. After that I started to wear thermal type gloves but soon found that
these were more dangerous than no gloves at all.
There are times when one does have to be careful in handling LN2 and these
in my experience have been with 1/ glass containers. 2/ pressurised
containers and 3/Accidental contact from surfaces at very cold temperatures.

I am sure that there have also been other experiences that we can learn from
so that we can understand how we can avoid them.

Ken Moran.

Moran Scientific Pty Ltd
P.O. Box 651
Goulburn NSW 2580 Australia
Tel 02 4844 4234 (International, 61 2 48444234)
Fax 02 4844 4291 (International, 61 2 48444291)
Email {kmoran-at-goulburn.net.au}
Web Page http://www.goulburn.net.au/~kmoran/
"Patience accomplishes its object, while hurry speeds to its ruin. Gulistan
1258"







From daemon Wed Jul 5 21:56:40 2000



From: Gordon Vrololjak :      gvrdolja-at-nature.Berkeley.EDU
Date: Wed, 5 Jul 2000 13:24:40 -0700 (PDT)
Subject: improving ISI SEM resolution

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Hello,
I've been cleaning and tweaking an older SEM we have in our
laboratory. It is an ISI DS-130. I managed to improve the resolution of
the lower stage from 2000x mag to 10000x magnification through cleaning
the final aperture, cleaning the column liner, replacing the
scintillators, and cleaning the anode. However, I would like to improve
it up to at least 30000x mag for the lower stage. Could anyone send me
some suggestions as how I may improve the performance of this SEM further?

I have an additional problem in using the upper stage of the SEM. It will
not focus above 300x in magnification even though it is properly set up
according to the manual. Anyone ever come across this problem before?
Gordon.

\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
Gordon Ante Vrdoljak Electron Microscope Lab
ICQ 23243541 http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
gvrdolja-at-nature.berkeley.edu UC Berkeley
phone (510) 642-2085 Berkeley CA 94720-3330
fax (510) 643-6207 cell (510) 290-6793




From daemon Wed Jul 5 21:56:41 2000



From: Huggins, Bradley J :      HUGGINBJ-at-bp.com
Date: Wed, 5 Jul 2000 16:49:09 -0500
Subject: Smart EDS?

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I have a customer who is considering the purchase of an SEM-EDS system. He
has asked me if there are any EDS programs out there that can take the EDS
data, and, given some minimal user input, provide a list of possible phases.
For example, Fe and S alone and in various proportions may yield - iron
sulfide FeS2 or FeS (pyrite or troilite) while the presence of a low level
of O might get a result of iron sulfite or maybe iron sulfate, etc.

I know this is out there past the edge of real usable analytical
information, but just thought I'd see if anything exists. I'm not talking
about anything real fancy like the EBSP techniqes, but basically an "expert"
EDS program that will return a list of potential phases or formulas based on
a little quantitative and specimen knowledge/input from the real expert.


Thanks in advance,
Brad Huggins





From daemon Thu Jul 6 11:04:09 2000



From: Steve Chapman :      PROTRAIN-at-CompuServe.COM
Date: Thu, 6 Jul 2000 03:31:06 -0400
Subject: improving ISI SEM resolution

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Hi,

About your DS130.

Like any SEM you need to be sure that you are not undersaturated and are
very accurately aligned for maximum signal: use the wave form mode.
Performance will be further improved if you run at between 5 and 8mm
working distance. Also, as the HT tank needs time to warm up to give
stability, do not try for performance until the HT has been on for at least
45 minutes.

The upper stage has two settings, high kV and low kV, where a shorter focal
length is usable. In each case the secimen MUST be in exactly the correct
position, not to high not too low as there is very little focal length
adjustment compared with "normal" out of lens SEM.

Good luck

Steve Chapman
Senior Consultant Protrain
For professional training and consultancy in EM world wide
www.emcourses.com
Tel +44 1280 814774 Fax +44 1280 914007



From daemon Thu Jul 6 11:04:10 2000



From: tellis2-at-hallmark.com
Date: Thu, 6 Jul 2000 07:52:06 -0500
Subject: liquid nitrogen

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I have also had trouble with exploding glass dewars, I now use the
plastic ones from the chem. suppliers for freezing samples..
For filling up our EDX I do that directly out of the 160L liquid
nitrogen tanks from our supplier, I use a special liquid nitrogen flexible
metal hose with a sintered brass end that lets the gas out the sides and
liquid out the end. The 160L tank sits on a special movable dolly, so I
unchain it and move it next to the EDX or whatever I need to fill up, it
saves spillage and no dewar is needed for EDX fillups.
I ordered the equipment from Southland Cryogenics, Inc. but I have
seen the same stuff at different cryogenic internet sites.

Terry Ellis




From daemon Thu Jul 6 11:06:25 2000



From: Matthew Lynn :      mlynn-at-miami.edu
Date: Thu, 06 Jul 2000 10:42:08 -0400
Subject: Reply: improving ISI SEM resolution

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Gordon,

I had to get 20,000 to 50,000x out of our DS-130 some years ago...I'll see what
I remember. Since I don't know the details of your setup, I will just throw
some questions at you. What spot size(s) are you using? What size column
aperture? I'm pretty sure you'll need to stick with spot HR, or at least STD
(Small) to reach 30000x. The TV image will be grainy, but photo scans will be
fine. If the image gets TOO noisy, try a larger column aperture. For a given
'Z', a certain combination of spot/aperture will work best. Is your scope on
air cushions? If so, are they inflated properly (not OVER inflated)? If not
on cushions, do you know what your floor vibration is like? Relocating to
ground floor did wonders for ours. You will also need quite high emission
current: setup the filament for high-res. (per the manual) and crank up the
bias as necessary. It's important to check the mechanical gun alignment
frequently...ours drifted quite a bit. Align gun, column aperture, focus,
correct astigmatism. In short, pretty standard stuff. I have some good
pictures at 50,000, not-so-good pictures at 150,000 and that's on the second
floor. Didn't have a functioning 1st stage, so I can't help you there. Wild
guess: range of objective current is not switching over properly when you
switch to 1st stage? Good luck.

Matt

Matthew J. Lynn, Ph.D.
Center for Advanced Microscopy
University of Miami
(305)284-4736
mlynn-at-miami.edu


On Wednesday, July 05, 2000 4:25 PM, Gordon Vrololjak
[SMTP:gvrdolja-at-nature.Berkeley.EDU] wrote:
} ------------------------------------------------------------------------
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} -----------------------------------------------------------------------.
}
}
} Hello,
} I've been cleaning and tweaking an older SEM we have in our
} laboratory. It is an ISI DS-130. I managed to improve the resolution of
} the lower stage from 2000x mag to 10000x magnification through cleaning
} the final aperture, cleaning the column liner, replacing the
} scintillators, and cleaning the anode. However, I would like to improve
} it up to at least 30000x mag for the lower stage. Could anyone send me
} some suggestions as how I may improve the performance of this SEM further?
}
} I have an additional problem in using the upper stage of the SEM. It will
} not focus above 300x in magnification even though it is properly set up
} according to the manual. Anyone ever come across this problem before?
} Gordon.
}
} \/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
} Gordon Ante Vrdoljak Electron Microscope Lab
} ICQ 23243541 http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
} gvrdolja-at-nature.berkeley.edu UC Berkeley
} phone (510) 642-2085 Berkeley CA 94720-3330
} fax (510) 643-6207 cell (510) 290-6793




From daemon Thu Jul 6 11:18:44 2000



From: Roberto Garcia :      rgarcia-at-unity.ncsu.edu
Date: Thu, 6 Jul 2000 11:20:04 -0400
Subject: MAT: Auger Microscope

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We are having problems with the hardware/software acquiring data from our
JEOL JAMP-30. The unit uses an old PDP11. We are wondering if anyone has a
similar system and can instruct us how to reformat the hard drive and reload
the software on this system. Any help would be appreciated. Thanks.
______________________________________________
Roberto Garcia
Senior Analyst, Metallography
NC State University / Analytical Instrumentation Facility
Campus Box 7531 Room 318 EGRC
1010 Main Campus Dr.
Raleigh, NC 27695-7531
(919) 515-8628
(919) 515-6965 Fax
rgarcia-at-unity.ncsu.edu
http://spm.aif.ncsu.edu/aif
http://spm.aif.ncsu.edu/asm/asm_home.htm
____________________________________________




From daemon Thu Jul 6 11:56:18 2000



From: Jane LaGoy :      jlagoy-at-bodycote-imt.com
Date: Thu, 6 Jul 2000 09:33:36 -0400
Subject: RE: Amray 1200 SEM

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If anyone wants Geoff Williams' Amray 1200, I have some sample holders and
tools left over from when we scrapped our Amray 1200 due to water damage.
The only cost would be shipping.

Jane L. LaGoy
Development Engineer
Bodycote IMT, Inc.
155 River Street
Andover, MA 01810
978-470-1620
jlagoy-at-bodycote-imt.com




From daemon Thu Jul 6 13:05:12 2000



From: Phillip Rutledge :      prutledge-at-ars.usda.gov
Date: Thu, 06 Jul 2000 06:49:00 -0600
Subject: stains

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Microscopists,

I know this isnt't an EM related question but I'm in search of a couple of LM staining procedures. I'm looking for a good silver stain (s) for looking at neural structures preferably not using chloral hydrate, and a good acid fast procedure for mycobacterium. If anyone can help, I'd appreciate it.

Thanks,

Phil Rutledge
USDA/ARS
voice: (410) 778-4136, 2120
fax: (410) 778-4399
e-mail: prutledge-at-ars.usda.gov



From daemon Thu Jul 6 13:32:53 2000



From: diane.a.ciaburri-at-gdds.com
Date: 7/3/00 10:28 PM
Subject: LN2 Spills

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I'm interested in why one would use a glass dewer for transporting LN2 (cost?).
Our transportation dewer is metal - don't know how many walls but I assume it's
got a vacuum between them. It's been in use for over 20 years and had its share
of bumps (I'm sure if it was glass, it'd have been replaced a few times). It's
made by Union Carbide, type UC-4.

It works great, has lasted 'forever' and doesn't have the safety issues of
glass. Is glass that much cheaper?

Diane Ciaburri
General Dynamics
Pittsfield MA


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I've avoided commenting on LN2 to this point, because one person became
quite offended at my comments the 4-5 years ago when this thread was
explored in a very similar manner. Ken's comments about shattered thermos
is an experience, though, I've shared too many times.

Besides being the glass thermos being a different kind of hazard after
breaking, a 5L dewar is very expensive. Just over a year ago I started
using a 2 gallon plastic drink dispenser from WalMart. Cost less than $10.

It lasted almost a year before the seal around the spigot failed. Of
course you can't store LN2 for any length of time but for transport between

tank and EDX detector, it does great. BTW, the top vent was permanently
removed so no pressure buildup could occur.

Chuck Butterick
Engineered Carbons, Inc.
Borger, TX

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Hi All,
there is a serious side to LN2 handling even though many points
discussed are valid. I have found most problems associated with LN2 are
caused by things other than the LN2 itself. I remember when I first started
using LN2 over 25 years ago I was filling a glass vacuum flask when it
exploded and filled the room I was in with instant fog, tinsel and plenty of
LN2 as a fine spray. I was unharmed apart from fine glass shards and Al foil
in my hair. I was wearing no protective gear. The LN2 had also been in my
hair as a fine spray thank goodness. I was amazed that I had no effect from
the LN2. After that I started to wear thermal type gloves but soon found that
these were more dangerous than no gloves at all.
There are times when one does have to be careful in handling LN2 and these
in my experience have been with 1/ glass containers. 2/ pressurised
containers and 3/Accidental contact from surfaces at very cold temperatures.

I am sure that there have also been other experiences that we can learn from
so that we can understand how we can avoid them.

Ken Moran.

Moran Scientific Pty Ltd
P.O. Box 651
Goulburn NSW 2580 Australia
Tel 02 4844 4234 (International, 61 2 48444234)
Fax 02 4844 4291 (International, 61 2 48444291)
Email {kmoran-at-goulburn.net.au}
Web Page http://www.goulburn.net.au/~kmoran/
"Patience accomplishes its object, while hurry speeds to its ruin. Gulistan
1258"




From daemon Thu Jul 6 14:21:23 2000



From: Geoff McAuliffe :      mcauliff-at-UMDNJ.EDU
Date: Thu, 06 Jul 2000 15:10:15 -0400
Subject: Re: stains

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Message-ID: {3964D997.963C5D7C-at-umdnj.edu}


Phillip Rutledge wrote:

} Microscopists,
}
} I know this isnt't an EM related question but I'm in search of a couple of LM staining procedures. I'm looking for a good silver stain (s) for looking at neural structures preferably not using chloral hydrate, and a good acid fast procedure for mycobacterium. If anyone can help, I'd appreciate it.
}
} Thanks,
}
} Phil Rutledge
} USDA/ARS
} voice: (410) 778-4136, 2120
} fax: (410) 778-4399
} e-mail: prutledge-at-ars.usda.gov

Dear Phil:

Without knowing more specifics, the Bodain method is a good silver stain for CNS. Details in any good histotechnology text. Can't help with mycobacterium.

Geoff

--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************





From daemon Thu Jul 6 15:36:12 2000



From: Purdy, Sam :      SPurdy-at-nationalsteel.com
Date: Thu, 6 Jul 2000 15:22:37 -0500
Subject: RE: Mat. Electropolishing.

Contents Retrieved from Microscopy Listserver Archives
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Dorrance:
Have look at ASTM Standard E 1558, Standard Guide for
Electropolishing of Metallographic Specimens.


Sam Purdy
Tech Center, National Steel Corp.
Trenton MI
} ----------
} From: McLean, Dorrance
} Sent: 30, June 2000, 3:21 PM
} To: 'Listserver'
} Subject: Mat. Electropolishing.
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
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} -----------------------------------------------------------------------.
}
}
} } Dear List,
} } Does any one have the dummies guide to electropolishing?? Some years
} ago
} } (3), I had a trouble shooting guide for electropolishing. This list had
} } about 9 or 10 items, problems /solutions on it and it was very
} } helpful...but it has found one of those very safe filing places (i.e. I
} } can't find it!) If any one has access to this list or something
} similar,
} } I would appreciate it if you could send me a copy.
} }
} } Many thanks in advance.
} }
} } Dorrance
} } PS I haven't been keeping up with the weather reports lately but it's 95
} } and clear in beautiful Livermore, California.
} }
}





From daemon Thu Jul 6 15:43:39 2000



From: hard-at-acsu.buffalo.edu
Date: Thu, 06 Jul 2000 16:47:38 -0500
Subject: Course Announcement II

Contents Retrieved from Microscopy Listserver Archives
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Phil
I wonder if Del Rio Hortega's silver stain would be good for your
purpose. See Goldblatt & Trump (1965) The application of del Rio
Hortega's Silver method to epon-embedded tissue. Stain Technology
40, 105-115.

Add 5ml of 10% AgNO3 to 20ml of 5% aq. sodium carbonate.
Horrible precipitate occurs. Add 0.88 ammonia dropwise until
precipitate has just cleared. Dilute to 45ml, filter.

Stain free-floating sections at 60oC for 30-120 min (use a solid
watch-glass with glass cover). Transfer sections to distilled water to
rinse, then dry down to slides from water at 60oC. reduce in 0.5%
formaldehyde for 30-60 seconds, wash in distilled water, fix in 5%
sodium thiosulphate (hypo), wash again and mount.

Chris

Date sent: Thu, 06 Jul 2000 06:49:00 -0600
} From: Phillip Rutledge {prutledge-at-ars.usda.gov}
To: Microscopy-at-sparc5.microscopy.com


Course Announcement

Title: Optical Microscopy and Imaging in the Biomedical Sciences

When: October 11 - October 19, 2000

Where: Marine Biology Laboratory, Woods Hole, MA, USA

Tuition: $2150 (Includes room and board, text, handouts, supplies)

Application Deadline: August 1, 2000

Admission application and information:
Carol Hamel, Admissions Coordinator
Marine Biological Laboratory
7 MBL Street
Woods Hole, MA 02543-1015
(508) 289-7401
Internet: admissions-at-mbl.edu
WWW: http://www.mbl.edu (Application forms available via Adobe Acrobat)

Course Director: Colin S. Izzard, State University of New York -at- Albany
Phone: [518] 442 - 4367
EMail: csizzard-at-csc.albany.edu

Course Description:

For Whom:
Designed primarily for research scientists, physicians, postdoctoral
trainees and advanced graduate students in animal, plant, medical and
material sciences. Non-biologists seeking a comprehensive introduction to
microscopy and video-imaging will benefit greatly from this course as well.
There are no specific prerequisites, but an understanding of the basic
principles of optics is desirable. Limited to 24 students.

The eight day course consists of lectures, laboratory demonstrations,
exercises and discussions that will enable the participant to obtain and
interpret microscope images of high quality, to perform quantitative optical
measurements, and to produce photographic and video records for
documentation
and analysis.

Topics to be covered include:
principles of microscope design and image formation
bright field, dark field, phase contrast, polarized light,
differential interference contrast, interference reflection, and
fluorescence microscopy
confocal scanning microscopy, multiphoton excitation fluorescence
microscopy and image deconvolution
digital image restoration and 3-D reconstruction
video imaging, recording, enhancement, and intensification
analog and digital image processing and analysis
fluorescent probes and ratiometric-imaging
laser tweezers and laser scissors

Applications to live cells will be emphasized; other specimens will be
covered as well.

Students will have direct hands-on experience with state-of-the-art
microscopes, video cameras, recorders and image processing equipment
provided by major optical and electronics companies. Instruction will be
provided by experienced staff from universities and industry.

Students are encouraged to bring their own biological (primary
cultures, cell lines, etc.) and material specimens and to discuss individual
research problems with the faculty.





From daemon Thu Jul 6 15:53:04 2000



From: smithde-at-valunet.com (Diane Smith)
Date: Thu, 6 Jul 2000 15:43:29 -0500
Subject: PCP positive control slides

Contents Retrieved from Microscopy Listserver Archives
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This isn't an EM question, but I'm hoping someone can give me an answer. I
need postive control slides for PCP fluorescence testing. If anyone knows of
any company that sells them, please let me know. Thanks





From daemon Thu Jul 6 15:59:02 2000



From: lpeterso987-at-hotmail.com
Date: Thu, 6 Jul 2000 15:49:02 -0500
Subject: student microscope adjustments

Contents Retrieved from Microscopy Listserver Archives
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Email: lpeterso987-at-hotmail.com
Name: lauren peterson
School: university of michigan

Question: I bought an old student microscope (3 objectives) from a biology
lab. It has a mirror and condenser lens (with iris) that can be focused up
and down. It doesn't seem to make much difference how the lens is adjusted
or how the iris is set -- both just seem to affect the brightness. Is
there a procedure for adjusting it to get the best images?

---------------------------------------------------------------------------





From daemon Thu Jul 6 16:07:30 2000



From: Michael Dunlap :      mrdunlap-at-ucdavis.edu
Date: Thu, 6 Jul 2000 13:59:48 -0700
Subject: improving ISI SEM resolution

Contents Retrieved from Microscopy Listserver Archives
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Gordon,

I can help you on both stages. Following the ISI manual can be like
many manuals, a little difficult.

The 1st stage is the simplest problem to fix. Select a tall or short
specimen holder, mount your sample on the adjustable base of the
holder. Turn the base until the top most portion of your sample is
at the same height as the top of the holder. Not above!! If you
selected the tall holder the 'Mode' button need to be on 2 and for
the short holder the 'Mode' button needs to be on 1. Go ahead and
put the sample and view. Increase the magnification up to 1 KX,
adjust the focus knob. You should be able to find focus.

As for the 2nd stage, it sounds as you are close in your alignments.
You should continue to practice. I find that it is difficult to
routinely expect to take micrographs above 20 KX. The general rule
around here is if you want to image above 20 KX go to the upper stage.

We use a LaB6 filament on our DS-130, I was imaging this week at 30KX
on the lower stage and at 100KX on the upper.

Mike
============================================================
Michael Dunlap office
(530) 752-0284
University of California lab (530) 752-5489
Chemical Engineering & Material Science Fax (530) 752-9554
110A EU-II
mrdunlap-at-ucdavis.edu
One Shields Ave.
http://www.chms.ucdavis.edu/
Davis CA, 95616
============================================================



From daemon Thu Jul 6 17:46:22 2000



From: =shAf= :      mshaf-at-darkwing.uoregon.edu
Date: Thu, 6 Jul 2000 15:36:46 -0700
Subject: RE: liquid nitrogen

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terry writes ...

} I have also had trouble with exploding glass dewars, ...
} For filling up our EDX I do that directly out of the
} 160L liquid nitrogen tanks from our supplier,
} I use a special liquid nitrogen flexible metal hose
} ...

The 160L dewar I fill from has a "nalgene" hose ... and I've never
had a problem with it. However, one plastic hose exploded on me while
filling, but it wasn't nalgene, and I can't remember what it was.
But, it does underline the use of wearing protective eyewear while
working with LN2!

=shAf= :o)




From daemon Thu Jul 6 19:16:37 2000



From: Rick Powell at Nanoprobes :      rpowell-at-nanoprobes.com
Date: Thu, 6 Jul 2000 20:03:11 -0500
Subject: TEM: Service contractors/water chiller wanted, NY region

Contents Retrieved from Microscopy Listserver Archives
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Hello List Members:

We are planning to acquire a Phillips 300 TEM for our lab located on Long
Island, NY. Does anyone have any recommendations for independent
service/maintenance contractors who could provide a service contract in
this area?

Also, we don't yet have a recirculating water chiller. If anyone has one
that they could sell to us, please let us know.

Thanks in advance,

Rick Powell
Nanoprobes, Incorporated


**********************************************************************
* - PLEASE NOTE OUR NEW ADDRESS - EFFECTIVE FEBRUARY 15, 2000 - *
* *
* NANOPROBES, Incorporated | US Toll-free: (877) 447-6266 *
* 95 Horse Block Road | Tel: (919) 510-0590 *
* Yaphank, NY 11980-9710, | Fax: (919) 510-0590 *
* USA | rpowell-at-nanoprobes.com *
* *
* NOW EASY TO FIND ON THE WEB: http://www.nanoprobes.com *
**********************************************************************





From daemon Thu Jul 6 19:16:58 2000



From: jim :      jim-at-proscitech.com.au
Date: Fri, 7 Jul 2000 09:55:58 +1000
Subject: RE: Procedure for handling LN2 - my summary

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This is my summary of that thread, reflecting my bias and my logic. Clearly
there were divergent opinions.
Everett had simply asked for a copy of "handling procedures". Hope that
somebody could help him.
The question though seems to be "is liquid nitrogen a dangerous substance"?
Obviously yes, but so is water - to wit drownings. Danger is a comparative
matter. The question "Is a written procedure required?" follows later.

Many common substances are dangerous when mishandled.
Petrol/ gas in my opinion is rather more dangerous than liquid nitrogen.
Gas is much more common than LN2, but few gas accidents are reported. LN2
accidents become folklore, not because of the greater danger of LN2 but because
those accidents demonstrate ingenious, applied stupidity (hi, that would make a
fine Uni course).
Although gas and its dangers are well known, people inhale it (brain damage and
death), start fires with it, store it in crummy plastic containers, siphon it
by mouth, use it to re kindle a fire etc.
I must agree though, gas is not a good comparison to LN2.

Boiling water is! Arguably its more dangerous than LN2 because it has no
insulating layer. So boiling water poured onto skin will burn, whereas LN2
would only do so in extreme cases. Pressurized LN2 must be compared with
pressurized boiling water, like in a pressure cooker or autoclave. Boiling
water is undeniably dangerous and many, many burns occur every day. Of course
its a very commonly used substance, but that means that all, except small
children would know of the danger and should use care.

Why then is boiling water not a tightly controlled substance and why is no
operator's license required to make a cup of coffee? Ask your safety officer.
Our discussion concerns LN2 use in laboratories, by people with more training
and understanding of physical properties than that of the general public. Why
should these people be required to submit a procedural outline for simple
tasks, such as transferring some LN2?
Who trained the safety officer to recognize that the procedure makes sense?
What is the cost benefit of introducing another bit of paper to the laboratory?
How is safety enhanced by a single task written procedure?
Most LN2 accidents are caused by lack of knowledge of the material's physical
properties, or temporary "insanity"?
A six minute briefing of LN2 properties and don'ts would benefits the neophyte
and safety rather more than a cabinet full of paper. The required written
procedure on decanting LN2 is a fig leaf for the safety officer. Unfortunately
safety officers have become part of a bureaucratic system and frequently they
are required to perform nonsensical tasks.

Overstated? Well, we now have in Australia legislation in place which makes the
importation, sale and trans shipping of Uranyl Acetate virtually impossible.
PST has given up on UA and I don't know of anybody who is willing to run that
legislative gauntlet for the sale of a few jars of UA!
This legislation applies in a country that produces thousands of tons of U
Yellowcake (radiological and chem toxicity similar to UA), were jumbo jets
carry 370 kg of U metal in the tail fin and were Americium smoke detectors are
handled by the public.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

} On Friday, June 30, 2000 6:35 AM, Everett Ramer
[S} MTP:Everett.Ramer-at-netl.doe.gov] wrote:

} I will getting my first SEM/EDS shortly and to obtain the required
operating permit from my safety group I need a written procedure for
handling LN2. Specifically I need a written procedure for filling the 3 L
dewar on the EDS detector from a 50 L dewar mounted on a cart. I plan to
make the transfer by using a lab source of N2 to pressurize the 50 L dewar
and have obtained all the valves and fittings required to do this from
another SEM lab. I would appreciate copies of the procedure.
} Thanks,
}
} Everett Ramer
} National Energy Technology Laboratory
} P.O. Box 10940, Cochrans Mill Road
} Pittsburgh, PA, USA 15236-0940
} Voice: 412-386-4920
} FAX: 412-386-4806
} ramer-at-netl.doe.gov



From daemon Fri Jul 7 03:32:43 2000



From: Steve Chapman :      PROTRAIN-at-CompuServe.COM
Date: Fri, 7 Jul 2000 04:19:42 -0400
Subject: Improving SEM Performance 2

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Again

Been thinking about your problem and of course suddenly thought that I did
not pass on the most important message last time!

If you want better performance from any SEM you must make sure that the
emission current is high enough. For DS130 use a current 100uA above the
standing current (the current that you have when the filament is off but
the selected high voltage is on).

Good luck

Steve Chapman
Senior Consultant Protrain
For professional training and consultancy in EM world wide
www.emcourses.com
Tel +44 1280 814774 Fax +44 1280 914007



From daemon Fri Jul 7 07:59:31 2000



From: Roberto Garcia :      rgarcia-at-unity.ncsu.edu
Date: Fri, 7 Jul 2000 08:49:23 -0400
Subject: MAT: Electropolishing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Try www.kaker.com they have and online demo of electropolishing procedures.
You can not search them but it will list all of the ones they have and then
you just have to look for your material of interest.
______________________________________________
Roberto Garcia
Senior Analyst, Metallography
NC State University / Analytical Instrumentation Facility
Campus Box 7531 Room 318 EGRC
1010 Main Campus Dr.
Raleigh, NC 27695-7531
(919) 515-8628
(919) 515-6965 Fax
rgarcia-at-unity.ncsu.edu
http://spm.aif.ncsu.edu/aif
http://spm.aif.ncsu.edu/asm/asm_home.htm
____________________________________________




From daemon Fri Jul 7 09:43:00 2000



From: William Tivol :      tivol-at-wadsworth.org
Date: Fri, 07 Jul 2000 10:26:45 -0400
Subject: Re: Procedure for handling LN2

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear List,
Here are my 2 cent's worth:
1) Teflon tubing does not become brittle at 77 K, so it
is the best inexpensive material for transferring LN2
safely. Using insulating foam tubing around the teflon
reduces LN2 boiling to a very acceptible degree.
2) Do not set up an automatic fill for LN2 for an EDS
detector; when we tried that, the fill valve froze open,
and the resulting LN2-fall broke the seal on the detec-
tor dewar, which then became convex on the bottom.
(We were able to fix it--I'll be happy to let anyone
who is interrested know how.) Naturally, this occurred
at the beginning of a long weekend; fortuitous, timely
discovery prevented a worse disaster.
And another penny's worth:
3) Glass dewars are not used because they're cheap;
they're used because the silvered glass is an excel-
lent insulator. They are very efficient for containing
or transporting LN2, and they hold up very well, unless
one drops a screwdriver into one. Stainless steel is
tougher, but less efficient and more expensive.
Yours,
Bill Tivol




From daemon Fri Jul 7 10:14:43 2000



From: Michelle.Taurino-at-aventis.com
Date: Thu, 6 Jul 2000 13:48:27 -0500
Subject: Removing Tol Blue from Epon embedded sections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello-

I am looking for any suggestions on how to remove Tol. blue from Epon
embedded sections?

I've tried warm water, 100% ethanol, and acetone.

Any other recommendations?

Will the Tol. blue interfere with UA/Pb staining if I do not remove the Tol.
Blue??

Thank you-
Michelle Taurino
Aventis Pharmaceuticals
Bioimaging and Molecular Histology
Tel-908-231-3357
Fax-908-231-3962
e-mail: Michelle.Taurino-at-aventis.com




From daemon Fri Jul 7 10:35:11 2000



From: Stanley L. Flegler :      flegler-at-pilot.msu.edu
Date: Fri, 07 Jul 2000 11:29:21 -0400
Subject: TEM Job Announcement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The following TEM position has been approved at Michigan State University.
The individual selected will supervise a newly funded 200 kV field emission
TEM fully equipped with EDS, PEELS, Gatan Image Filter, STEM, digital
imaging, etc.
Stanley L. Flegler, Acting Director
Center for Advanced Microscopy
Michigan State University


Full-time transmission electron microscopist. This is a
teaching/service/support position at the Center for Advanced Microscopy at
Michigan State University. CAM is the central microscopy laboratory for
the MSU campus, serving users from a wide variety of disciplines. The
appointment will be in the Academic Specialist category, one of the
academic support ranks at the University. The appointment will be a 12
month, annual year appointment in the continuing appointment system.
Salary will be commensurate with experience. Required: A Ph.D. in the
physical sciences or a field of engineering and a minimum of three years
experience in TEM instrument operation, maintenance, physical science
sample preparation, and teaching. Experience in a multi-user facility and
familiarity with biological TEM sample preparation is a plus. The
individual must have a demonstrated competence in and a strong commitment
to graduate level instruction and be willing to assist others in planning
their research programs and/or sample preparation. U.S. citizenship is not
required; applicants who are not U.S. citizens or permanent residents must
provide documentation evidencing employment authorization in the United
States. The position begins effective Spring 2001. Submit a Curriculum
Vita, transcripts of academic training, a statement describing your
interest in the position, evidence of teaching ability, and arrange for
three letters of recommendation to be sent to: Dr. Karen L. Klomparens,
Chair, Search Committee, Center for Advanced Microscopy, B4 Center for
Integrated Plant Systems, Michigan State University, East Lansing, MI
48824. Applications are due by Sept. 18, 2000. MSU is an Affirmative
Action/Equal Opportunity Institution.



From daemon Fri Jul 7 13:22:51 2000



From: Chris Jeffree :      cjeffree-at-srv0.bio.ed.ac.uk
Date: Fri, 7 Jul 2000 19:04:12 +0100
Subject: Dewars for handling LN2

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I use "dewars" made of foamed polystyrene for transferring small
volumes of LN2, and for any work where there is a risk of dropping
heavy metal objects into the dewar. They are safe, light, robust, and
inexpensive, although they do not have anything approaching the
holding time of glass dewars. They should be obtainable through
most EM supply houses. If you have difficulty sourcing them,
contact me offline.

Just for the record, I have used glass dewars for LN2 for more than
30 years, and have never known one to break under thermal shock.

Chris Jeffree
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Dr Chris Jeffree
University of Edinburgh
Biological Sciences EM Facility
Daniel Rutherford Building
King's Buildings EDINBURGH EH9 3JH
Tel: +44 (0) 131 650 5345
FAX: +44 (0) 131 650 6563

Inveresk Cottage, 26 Carberry Road,
Inveresk, Musselburgh, Midlothian EH21 8PR, UK
Tel. +44 (0) 131 665 6062 / Mobile 0410 585 401
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~



From daemon Fri Jul 7 13:34:34 2000



From: Deanne Hoenscheid :      dlh3-at-lehigh.edu
Date: Fri, 07 Jul 2000 14:24:51 -0400
Subject: Position Available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Please note the following job posting at Lehigh University:

RESEARCH ENGINEER

The Department of Materials Science and Engineering at Lehigh University
is seeking a Research Engineer. This position will be responsible for
high spatial resolution x-ray mapping of segregants in alloys using the
VGHB603; theoretical spectral simulation using desktop spectrum
analyzer; and zeta factor mapping and quantification.

Qualified candidates should have Ph.D. in Materials Science or related
field with at least 3 years of postdoctoral experience. Experience with
300KeV Field Emission Gun STEMs is essential.

Anticipated starting date is April 1, 2001. Resumes should be sent to
Deanne Hoenscheid, Materials Research Center, Lehigh University, 5 E.
Packer Avenue, Bethlehem, PA 18015. Lehigh University is an AA/EOE.






--
*********************************************************
Deanne L. Hoenscheid Phone: (610) 758-3863
Materials Research Center Fax: (610) 758-3526
462 Whitaker Laboratory E-mail: dlh3-at-lehigh.edu
5 E. Packer Avenue
Bethlehem, PA 18015
*********************************************************



From daemon Fri Jul 7 14:11:45 2000



From: milesd-at-us.ibm.com
Date: Fri, 7 Jul 2000 15:03:08 -0400
Subject: Re: Dewars for handling LN2

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I believe the problems arise when scratches occur from filling
and handling the glass dewars. These can be so fine, as to be
difficult to see. (when cutting glass, a fine, chip free scribe does
a better job than a heavy one) When the conditions are just
right (stress from cooling down, or tipping the dewar up), the
vacuum bottle will implode.

We have some old metal outside, plastic inside dewars for
transferring LN2 from the storage/supply dewar, to the system
dewar. They are not used to store LN2, but are good and
rugged for all of the handling they get. They were here when
I started, so I do not know where they came from.

Darrell




From daemon Fri Jul 7 16:16:00 2000



From: Bryan Tracy :      bryant-at-brahms.amd.com
Date: Fri, 7 Jul 2000 13:56:12 -0700
Subject: FIB opening at AMD Sunnyvale

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



We are please to offer the following position at AMD Sunnyvale



REQUIRED EXPERIENCE

Candidate should have a strong Materials Science education and extensive
exposure to electron microscopy. At least two years industrial experience
is preferred except if candidate did MS or PhD thesis work in electron
microscopy. Good team skills are required as well as the capability to
handle changes in workflow.


ESSENTIAL JOB FUNCTIONS

Be AMD Sunnyvale's Focussed Ion Beam (FIB) expert. Perform delicate FIB
device modifications. Develop sample preparation techniques for
Transmission Electron Microscopy using FIB. Represent Materials Technology
Development (MTD) department on problem solving teams. Set and drive MTD's
FIB roadmap.


Please contact Bryan Tracy


bryan.tracy-at-amd.com
408-749-4819










Bryan Tracy
Materials Technology Development
Advanced Micro Devices
915 Deguigne Ave, Mailstop 143
Sunnyvale, Ca, 94088





From daemon Fri Jul 7 17:41:30 2000



From: Joyce Kotzuk :      JKotzuk-at-salud.unm.edu
Date: Fri, 07 Jul 2000 16:28:06 -0600
Subject: TEM-Immuno methods review papers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,
I am looking for a good review paper concerning immuno EM methods. There was a book suggested recently "Fine Structure Immunocytochemistry", which I am trying to locate through my library here at UNM, but I am really more interested in a few good papers.
Thanks in advance,
Joyce A. Kotzuk
Univ. of New Mexico pathology dept.




From daemon Fri Jul 7 18:32:41 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Fri, 7 Jul 2000 18:20:56 -0500
Subject: Re: student microscope adjustments

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


}
} Dear Lauren,
}
} While it looks to you like both of these adjustments affect just
} brightness, they are actually have a profound influence on the coherence of
} the light hitting the sample. To test, move the objectives out of position
} and place a piece of white card vertically on the stage so that you can
} catch the beam emerging from the condenser. Open and close the condenser
} and watch the beam go from a wide angle to a narrow pencil. Open the
} condenser then focus it up and down. Notice how the location of the cone
} changes.
}
} The narrower the beam, the more coherent the light impinging on the sample.
} The more coherent, the more visible the diffraction effects at edges. For
} nearly invisible specimens like cheek cells, the diffraction will enhance
} edge information. If you go to far, you will see the bright and dark
} fringes which result from the constructive and destructive interference of
} diffracted waves hitting the edges.
}
} Hope this is helpful.
}
} Best regards,
} Barbara Foster,President
} Microscopy/Microscopy Education
} 125 Paridon Street Suite 102
} Springfield, MA 01118-2130
} PH: 413-746-6931 FX: 413-746-9311 email: MME-at-map.com
} Website: www.MME-Microscopy.com/education
}
}
}
} At 03:49 PM 7/6/00 -0500, you wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America





From daemon Fri Jul 7 21:21:13 2000



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Fri, 07 Jul 2000 19:04:40 -0700
Subject: Fwd: Re: Procedure for handling LN2

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Yes, I did show my kids some spectacular show with LN2. You could make
beautiful white clouds pouring LN2 in warm water. The most successful
demonstration was, when I was using 1 liter calibrated flask with long
neck. White cloud coming from the neck forms kind of shape my kids called
jinn from "1000 +1 night Shaherezad". We also used LN2 in some shows at
traditional New Year Party at my Institute in Russia. One time I was using
regular thermos filled with LN2 to transport my samples from Novosibirsk to
Moscow (8 hours fly). People at Novosibirsk were short in dry ice, we were
using LN2 instead (smile). You could also put a small piece of dry ice
into 1.5 ml Eppendorf tube and place it under the door of your boss (a
couple of tubes are better). They are blow out with nice sound like a shot
from good pistol (but less dangerous). Honestly speaking, I did it one time.


} Now there's a thought. To digress a little, we once had an instance where a
} guy came down to the SEM lab and asked if he could borrow a 5 litre dewar
} to take some LN2 home and show his kids. When asked for more details his
} game plan was to sit the dewar on the floor on the passenger side of the
} car so that he could make sure that it wouldn't fall over. The answer was
} "No, perhaps you should bring the kids to the LN2...."
}
} The point being that even though LN2 use might seem less of a risk than
} boiling water when used "properly" and "common sense" is applied, it cannot
} be assumed without question that everyone's initial mental picture behind
} these two terms is exactly the same!!

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu






From daemon Sat Jul 8 08:08:03 2000



From: Alan Berginc :      aberginc-at-cressington.com
Date: Sat, 8 Jul 2000 07:52:56 -0500
Subject: Plexiglas implosion guards for Bell Jars

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




}
} * Hello to all.
} * I am looking for a supplier of acrylic (Plexiglas) implosion guards for
} a 12
} * inch diameter glass bell jar for a vacuum evaporator. I can only seem
} to find
} * the wire mesh or expanded metal types. I would like to know if anyone
} has such
} * a thing or knows who fabricates them or supplies them off the shelf.
}
} * Reply on line or off line direct to me at aberginc-at-cressington.com.
}
}

Regards.........Alan Berginc
Cressington Scientific, INC





From daemon Sat Jul 8 09:36:09 2000



From: jim :      jim-at-proscitech.com.au
Date: Sun, 9 Jul 2000 00:25:43 +1000
Subject: FW: Removing Tol Blue from Epon embedded sections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Acid alcohol removes tol blue and many other stains. Use 50 to 70% Ethanol with
a 1ml of 1N HCl per 20ml, then rinse with water. When used quickly only little
destaining occurs and the tol blue differentiates giving nice pinks and blues
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Friday, July 07, 2000 4:48 AM,
"Michelle.Taurino-at-aventis.com"-at-sparc5.microscopy.com
[SMTP:"Michelle.Taurino-at-aventis.com"-at-sparc5.microscopy.com] wrote:
}
}
}
} Hello-
}
} I am looking for any suggestions on how to remove Tol. blue from Epon
} embedded sections?
}
} I've tried warm water, 100% ethanol, and acetone.
}
} Any other recommendations?
}
} Will the Tol. blue interfere with UA/Pb staining if I do not remove the Tol.
} Blue??
}
} Thank you-
} Michelle Taurino
} Aventis Pharmaceuticals
} Bioimaging and Molecular Histology
} Tel-908-231-3357
} Fax-908-231-3962
} e-mail: Michelle.Taurino-at-aventis.com
}




From daemon Sat Jul 8 10:42:36 2000



From: Susnitzky, David :      david.susnitzky-at-intel.com
Date: Sat, 8 Jul 2000 08:31:58 -0700
Subject: unsubscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


unsubscribe




From daemon Sat Jul 8 14:58:13 2000



From: rahul padmakar panat :      panat-at-students.uiuc.edu
Date: Sat, 8 Jul 2000 14:47:44 -0500 (CDT)
Subject: SEM straining stage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,
I am looking for
1) possible SEM accessory provider's list (that can
supply straining stages) and
2) interaction with somebody with some prior
experience with straining stages in SEM;
since I want to perform the following experiment:
Compression of a coated superally cube with adjustable
dimensions (e.g. 10*10*6mm) either by direct compression or
by bending. If possible, I would also like to repeat this
experiment at various temperatures (say up to 1200C).
The above experiment is to be observed under an SEM
which is Hitachi S800.

Thanks
Rahul Panat
------------------------------------------------------------------------
Rahul Panat
Dept of Theoretical & Applied Mechanics
Univ of Illinois -at- Urbana-Champaign
panat-at-students.uiuc.edu
------------------------------------------------------------------------




From daemon Sat Jul 8 16:57:15 2000



From: Chris :      cholp-at-ncweb.com
Date: Sat, 8 Jul 2000 16:34:22 -0500
Subject: Nitrogen in presence of titanium

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am working on a resolving a bit of a sticky problem. A customer wants
to confirm or disprove whether complex Ti/W/Nb carbo-nitrides in a Ni base
alloy do indeed contain nitrogen. I have 2 SEMs, both with WDS. The
better of the 2 uses an LSM080 crystal for the nitrogen wavelengths, the
other has an LOD. My approach so far has been to establish peak ratios
on cp titanium and a TiN standard. Some of the numbers look promising, but
I suspect an influence on the ratios due to the presence of: W, Nb, and
proximity of the matrix. The carbides are small and needle shaped,
perhaps 1-2 by 10-15 microns. Any comments or suggestions would be
greatly appreciated! Thanks, Chris Holp Reply through: Hm:
{mailto:cholp-at-ncweb.com} cholp-at-ncweb.com , Wk:
{mailto:holpcr-at-earthlink.net} holpcr-at-earthlink.net , or through the group.






From daemon Sun Jul 9 09:29:02 2000



From: Dmitri Lapotko :      ld-at-hmti.ac.by
Date: Sun, 9 Jul 2000 17:11:13 +0300
Subject: Fw: LM:need 12-bit CCD camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Dear group,

I am looking for low-budget camera w/board:
-CCD digital monochrome (630 nm);
-12-14 bit dynamic range;
-min format 512x512;
-shutter;
-external triggering;
-1 frame/s min rate;
-PC-compatible board.
Does anybody have any used equipment for sale?
Any input on the sources for inexpencive new cameras with
such specs will be appreciated.

Best regards

Dmitri Lapotko
Luikov Heat and Mass Transfer Institute
Minsk, Belarus

tel:(375172)842483
fax:(375172)842486
ld-at-ns1.hmti.ac.by




From daemon Sun Jul 9 16:59:26 2000



From: Garrison, Becky :      becky.garrison-at-jax.ufl.edu
Date: Sun, 9 Jul 2000 17:40:35 -0400
Subject: stains

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We use the Sevier-Munger modification found in Sheehan's Histotechnology
text for our neuropath stains. It is more reproducible than the Bodian
stain. The protocol is lengthy; will fax if you don't have access to the
text.

Becky Garrison
Dept. Pathology
Shands Jacksonville
Jacksonville, FL
904-549-6237

-----Original Message-----
} From: Phillip Rutledge [mailto:prutledge-at-ars.usda.gov]
Sent: Thursday, July 06, 2000 8:49 AM
To: Microscopy-at-sparc5.microscopy.com


Microscopists,

I know this isnt't an EM related question but I'm in search of a couple of
LM staining procedures. I'm looking for a good silver stain (s) for
looking at neural structures preferably not using chloral hydrate, and a
good acid fast procedure for mycobacterium. If anyone can help, I'd
appreciate it.

Thanks,

Phil Rutledge
USDA/ARS
voice: (410) 778-4136, 2120
fax: (410) 778-4399
e-mail: prutledge-at-ars.usda.gov



From daemon Mon Jul 10 07:55:10 2000



From: Ladd Research :      ladres-at-worldnet.att.net
Date: Mon, 10 Jul 2000 08:41:08 -0400
Subject: Re: Plexiglas implosion guards for Bell Jars

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Alan Berginc wrote:
}
} ------------------------------------------------------------------------} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.}
} }
} } * Hello to all.
} } * I am looking for a supplier of acrylic (Plexiglas) implosion guards for
} } a 12
} } * inch diameter glass bell jar for a vacuum evaporator. I can only seem
} } to find
} } * the wire mesh or expanded metal types. I would like to know if anyone
} } has such
} } * a thing or knows who fabricates them or supplies them off the shelf.
} }
} } * Reply on line or off line direct to me at aberginc-at-cressington.com.
} }
} }
}
} Regards.........Alan Berginc
} Cressington Scientific, INC


Dear Alan,

Please check our web site, http://www.laddresearch.com for catalog
# 30110 which I believe is what you are looking for.

Debbie Sicard
--

LADD RESEARCH
131 Dorset Lane
Williston, VT 05495 USA

TEL 1-800-451-3406 (US) or 1-802-878-6711 (anywhere)
FAX 1-802-878-8074
e-mail ladres-at-worldnet.att.net

ALL NEW WEB SITE UP NOW AT OUR NEW URL:

http://www.laddresearch.com



From daemon Mon Jul 10 10:02:18 2000



From: hpadams :      hpadams-at-bcm.tmc.edu
Date: Mon, 10 Jul 2000 09:51:25 -0500
Subject: resin mixer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


EM people/, does anyone know of a supplier who sells the Zoakes Teflon Helix
stirrer? I believe that was its name. It provided an efficient way to mix
resins without entraining air into the mix. I haven't seen it listed in the
any of the recent catalogues.

Hank Adams
Manager
Integrated Microscopy Core
Molecular and Cellular Biology
Baylor College of Medicine
Houston, Tx 77030




From daemon Mon Jul 10 10:46:19 2000



From: Nafisa Ghori :      nghori-at-stanford.edu
Date: Mon, 10 Jul 2000 08:36:53 -0700
Subject: Re: Removing Tol Blue from Epon embedded sections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 01:48 PM 7/6/00 -0500,
Michelle.Taurino-at-aventis.com"-at-sparc5.microscopy.com wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Just wash you slids with 100% ETHYL ALCOHOL and after that wash with
dd water it should do it





From daemon Mon Jul 10 10:50:14 2000



From: Steve Johnson :      stephen.johnson-at-anlw.anl.gov
Date: Mon, 10 Jul 2000 09:47:46 -0600
Subject: TEM microscopist position at Argonne National Laboratory-West

Contents Retrieved from Microscopy Listserver Archives
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MATERIAL SCIENTIST

We are seeking a Material Scientist to perform TEM and SEM characterization
of materials, particularly nuclear waste forms intended for repository
disposal. Measure and evaluate the properties of all types. Participate in
planning, developing, and implementing materials research to develop ceramic
materials for waste forms and process equipment components.

The successful candidate will:
o Conduct and direct materials characterization, with special emphasis on
TEM examination of nuclear waste forms.
o Analyze, evaluate, document, and communicate results of experiments and
programs.
o Contribute to and participate in internal and external Laboratory
technical reporting and presentations.
o Collaborate with other technical staff in the specialty area of materials
science.
o Ensure that the TEM is properly maintained.

We are seeking candidates with:
o Ph.D. or M.S. in Materials Science, Ceramics or related field.
o US Citizenship and ability to obtain DOE security clearance required.

Argonne offers a professional environment, excellent working conditions and
fringe benefits. Please mail or e-mail your resume, quoting Ref: Material
Scientist, ANL-W-1234, to:
Argonne National Laboratory-West
Human Resources
P.O. Box 2528
Idaho Falls, ID 83403-2528.
E-mail: hr-at-anlw.anl.gov
Equal Opportunity Employer



From daemon Mon Jul 10 11:08:09 2000



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Mon, 10 Jul 2000 08:57:05 -0700
Subject: Re: Nitrogen in presence of titanium

Contents Retrieved from Microscopy Listserver Archives
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Deear Chris,
You might want to look at my article on N in Ti in the August, 1999 issue of
Microscopy Today. By looking carefully at the peak shape of pure Ti vs. TiN
, I found the optimum location on the peak to test for the presence of N.
The W and Nb should not interfere at this wavelength and you should be able
to see if there is N above a detection limit of less than 0.5 weight
percent. The other thing that might help is to do an extraction replica of
the inclusions, so that they are free of the Ni matrix. Lay this down on a
carbon stub to reduce the background and improve your peak-to-background ratio.
At 04:34 PM 7/8/00 -0500, you wrote:

} I am working on a resolving a bit of a sticky problem. A customer wants
} to confirm or disprove whether complex Ti/W/Nb carbo-nitrides in a Ni base
} alloy do indeed contain nitrogen. I have 2 SEMs, both with WDS. The
} better of the 2 uses an LSM080 crystal for the nitrogen wavelengths, the
} other has an LOD. My approach so far has been to establish peak ratios
} on cp titanium and a TiN standard. Some of the numbers look promising, but
} I suspect an influence on the ratios due to the presence of: W, Nb, and
} proximity of the matrix. The carbides are small and needle shaped,
} perhaps 1-2 by 10-15 microns. Any comments or suggestions would be
} greatly appreciated! Thanks, Chris Holp Reply through: Hm:
} {mailto:cholp-at-ncweb.com} cholp-at-ncweb.com , Wk:
} {mailto:holpcr-at-earthlink.net} holpcr-at-earthlink.net , or through the group.
Good luck,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca




From daemon Mon Jul 10 11:28:43 2000



From: Jorge P. :      jpadilla-at-blue.weeg.uiowa.edu
Date: Mon, 10 Jul 2000 11:20:05 -0500 (CDT)
Subject: unsuscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


unsuscribe

Jorge P.






From daemon Mon Jul 10 11:44:05 2000



From: Smartech :      smartech-at-javanet.com
Date: Mon, 10 Jul 2000 12:44:00 -0400
Subject: Another digital camera question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I recently acquired a unitron MeC3-2313. It is a 30 year old, mono-ocular,
metallurgical, inverted, optical microscope. The images are high
resolution, bright, and the stage works well. I want to connect it to a
digital camera. It came w/ a T-mount and I can get a C-mount from unitron
for $625, I suppose I need a digital camera that has a removable lens. I
have seen specs for a the SV Micro ($2200) and the Pixera ($1200). Does
anybody have any other cameras they would like to suggest or experience w/
these camera? Is the overall image quality good for theses two cameras? I
am afraid that the resolution would "not be there" in the color mode for the
Pixera. I am not sure I want a consumer camera that has been adapted for a
microscope like the Nikon and Kodak, since they include a lens that cannot
be re-moved and therefore the minimum magnification work would be "upped" by
3X, if I could go thru the ocular. Plus, I am not sure I can get adaptors
for my scope anyway. I would also like to keep my only ocular open, so I
can skip the whole digital phase while doing sample inspection. Like to
keep the entire endevor under $3K.

Thanks

Ric




From daemon Mon Jul 10 14:20:37 2000



From: Walck, Scott D. :      walck-at-ppg.com
Date: Mon, 10 Jul 2000 15:10:34 -0400
Subject: RE: Another digital camera question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have both of those cameras attached to two different stereomicroscopes.
The SV-micro produces a bit better picture but is not as easy to work with
especially getting the exposure just right. I like using the Pixera -its
quick and easy. We don't do anything critical with these cameras however.
But they work well for the price.

For adapters, contact the folks at Diagnostic Instruments. Their web site
is
http://www.diaginc.com/ccdguide.htm


-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)



} -----Original Message-----
} From: Smartech [mailto:smartech-at-javanet.com]
} Sent: Monday, July 10, 2000 12:44 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Another digital camera question
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
}
} --------------------------------------------------------------
} ---------.
}
}
}
} I recently acquired a unitron MeC3-2313. It is a 30 year
} old, mono-ocular,
} metallurgical, inverted, optical microscope. The images are high
} resolution, bright, and the stage works well. I want to
} connect it to a
} digital camera. It came w/ a T-mount and I can get a C-mount
} from unitron
} for $625, I suppose I need a digital camera that has a
} removable lens. I
} have seen specs for a the SV Micro ($2200) and the Pixera
} ($1200). Does
} anybody have any other cameras they would like to suggest or
} experience w/
} these camera? Is the overall image quality good for theses
} two cameras? I
} am afraid that the resolution would "not be there" in the
} color mode for the
} Pixera. I am not sure I want a consumer camera that has been
} adapted for a
} microscope like the Nikon and Kodak, since they include a
} lens that cannot
} be re-moved and therefore the minimum magnification work
} would be "upped" by
} 3X, if I could go thru the ocular. Plus, I am not sure I can
} get adaptors
} for my scope anyway. I would also like to keep my only
} ocular open, so I
} can skip the whole digital phase while doing sample
} inspection. Like to
} keep the entire endevor under $3K.
}
} Thanks
}
} Ric
}
}



From daemon Mon Jul 10 15:01:24 2000



From: David Cugier :      david.cugier-at-abbott.com
Date: Mon, 10 Jul 2000 14:54:06 -0500
Subject: NIST traceable TEM magnification standards.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am currently searching for a NIST traceable TEM mag. standard. The reason
for a NIST traceable standard is that our EM facility is in transition to
becoming GMP regulated. I have used the waffle grating replica and catalase
crystals in the past and prefer this type of standard but they are not NIST
traceable. I am aware that NIST offers polystyrene nanosphere particles for
sizing but was hoping to avoid using because of the difficulty in producing a
uniform monolayer without aggregate particles. I am also aware that there is
a product called MAGICAL and am still waiting from a response from NIST as to
whether or not it is traceable. Does anyone know of any 3mm prepared grid
for use as a TEM mag std traceable to NIST. I am performing magnification
checks from 1,000X through 200,000X. Any info would be much appreciated.


David Cugier
Assistant Scientist
Microscopy and Microanalysis
Abbott Laboratories
Abbott Park, Illinois 60064
847-938-6725



From daemon Mon Jul 10 17:06:28 2000



From: John Foust :      jfoust-at-threedee.com
Date: Mon, 10 Jul 2000 16:42:53 -0500
Subject: Zeiss OPMI-1 available

Contents Retrieved from Microscopy Listserver Archives
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I have a Zeiss OPMI-1 binocular microscope. I got it as part of an
auction lot. I believe it was used by the ENT department of a medical
school.

If it can be restored to working condition, that would be great.
If not, I'd be glad to sell it.

I don't know what it's worth, or really what it needs to be restored
to working order. If it were pristine and on a stand, that would be
another matter, it's clear. On the other hand, I don't know
exactly which model it is.

I have several images of it at http://www.threedee.com/sales/zeiss.html .
Any assistance would be appreciated!

- John




From daemon Mon Jul 10 17:43:16 2000



From: Walck, Scott D. :      walck-at-ppg.com
Date: Mon, 10 Jul 2000 18:29:10 -0400
Subject: RE: NIST traceable TEM magnification standards.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I do not know what GMP certification, but our lab is QS9000 certified. That
is the automotive ISO9001 equivalent. I use the Mag-i-cal sample since all
lengths of the sample were traceable to lattice spacings of the Si crystal.
John McCaffrey has a letter from NIST, a copy of which that can accompany
the Mag-i-cal sample, that states that they do not currently have a standard
for calibrating TEMs nor do they have a calibration for the crystalline
lattice spacings of Si. They state that the crystalline lattice is an
intrinsic property of a material, well characterized, well documented in the
literature, and known to 6 decimal places. I have placed this letter in my
calibration standards file along with the certification that comes with it.
If the GMP is anything like ISO or Qs certification, what is important is it
is that you have the paper work and procedures documented and that you
follow them.

This should be close enough for government work.


-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)



} -----Original Message-----
} From: David Cugier [mailto:david.cugier-at-abbott.com]
} Sent: Monday, July 10, 2000 3:54 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: NIST traceable TEM magnification standards.
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
}
} --------------------------------------------------------------
} ---------.
}
}
} I am currently searching for a NIST traceable TEM mag.
} standard. The reason
} for a NIST traceable standard is that our EM facility is in
} transition to
} becoming GMP regulated. I have used the waffle grating
} replica and catalase
} crystals in the past and prefer this type of standard but
} they are not NIST
} traceable. I am aware that NIST offers polystyrene
} nanosphere particles for
} sizing but was hoping to avoid using because of the
} difficulty in producing a
} uniform monolayer without aggregate particles. I am also
} aware that there is
} a product called MAGICAL and am still waiting from a response
} from NIST as to
} whether or not it is traceable. Does anyone know of any 3mm
} prepared grid
} for use as a TEM mag std traceable to NIST. I am performing
} magnification
} checks from 1,000X through 200,000X. Any info would be much
} appreciated.
}
}
} David Cugier
} Assistant Scientist
} Microscopy and Microanalysis
} Abbott Laboratories
} Abbott Park, Illinois 60064
} 847-938-6725
}



From daemon Mon Jul 10 20:52:00 2000



From: RetTek2000-at-aol.com
Date: Mon, 10 Jul 2000 21:41:00 EDT
Subject: unsubscribe

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From daemon Mon Jul 10 22:25:10 2000



From: Kalman Rubinson :      kr4-at-is2.nyu.edu
Date: Mon, 10 Jul 2000 23:15:07 -0400 (EDT)
Subject: Re: unsubscribe

Contents Retrieved from Microscopy Listserver Archives
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See the message

On Mon, 10 Jul 2000
RetTek2000-at-aol.com-at-sparc5.microscopy.com wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
}






From daemon Tue Jul 11 09:43:26 2000



From: Jorge P. :      jpadilla-at-blue.weeg.uiowa.edu
Date: Tue, 11 Jul 2000 09:25:46 -0500 (CDT)
Subject: unsubscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html










From daemon Tue Jul 11 10:06:35 2000



From: mtl :      mtl-at-njcc.com
Date: Tue, 11 Jul 2000 10:57:01 -0400
Subject: Re: GMP vs ISO9000

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


There is no GMP/GLP certification given by the FDA nor are there FDA certified
laboratories. There is only compliance and being subjected to an unannounced
FDA audit. Where the various ISO9000 standards are orientated towards
improvement of quality and yield, GMP is a different standard orientated
towards having the sample used in a legal trial. For example, a company may
tolerate 99% yield for economic reasons, however, how many babies on a
percentage basis is a doctor allowed to drop a year. Failure to comply with
ISO9000 may have some economic consequencies. Caught failing to comply with
GMP, the FDA will consider the medical device or drug adulterated until proven
otherwise.

J. Roy Nelson, Ph.D.
Material Testing Laboratory
mtl-at-njcc.com


"Walck, Scott D." wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} I do not know what GMP certification, but our lab is QS9000 certified. That
} is the automotive ISO9001 equivalent. I use the Mag-i-cal sample since all
} lengths of the sample were traceable to lattice spacings of the Si crystal.
} John McCaffrey has a letter from NIST, a copy of which that can accompany
} the Mag-i-cal sample, that states that they do not currently have a standard
} for calibrating TEMs nor do they have a calibration for the crystalline
} lattice spacings of Si. They state that the crystalline lattice is an
} intrinsic property of a material, well characterized, well documented in the
} literature, and known to 6 decimal places. I have placed this letter in my
} calibration standards file along with the certification that comes with it.
} If the GMP is anything like ISO or Qs certification, what is important is it
} is that you have the paper work and procedures documented and that you
} follow them.
}
} This should be close enough for government work.
}
} -Scott
}
} Scott D. Walck, Ph.D.
} PPG Industries, Inc.
} Glass Technology Center
} Guys Run Rd. (packages)
} P. O. Box 11472 (letters)
} Pittsburgh, PA 15238-0472
}
} Walck-at-PPG.com
}
} (412) 820-8651 (office)
} (412) 820-8161 (fax)
}
} } -----Original Message-----
} } From: David Cugier [mailto:david.cugier-at-abbott.com]
} } Sent: Monday, July 10, 2000 3:54 PM
} } To: Microscopy-at-sparc5.microscopy.com
} } Subject: NIST traceable TEM magnification standards.
} }
} }
} } --------------------------------------------------------------
} } ----------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society
} } of America
} } To Subscribe/Unsubscribe -- Send Email to
} } ListServer-at-MSA.Microscopy.Com
} } On-Line Help
} } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} }
} } --------------------------------------------------------------
} } ---------.
} }
} }
} } I am currently searching for a NIST traceable TEM mag.
} } standard. The reason
} } for a NIST traceable standard is that our EM facility is in
} } transition to
} } becoming GMP regulated. I have used the waffle grating
} } replica and catalase
} } crystals in the past and prefer this type of standard but
} } they are not NIST
} } traceable. I am aware that NIST offers polystyrene
} } nanosphere particles for
} } sizing but was hoping to avoid using because of the
} } difficulty in producing a
} } uniform monolayer without aggregate particles. I am also
} } aware that there is
} } a product called MAGICAL and am still waiting from a response
} } from NIST as to
} } whether or not it is traceable. Does anyone know of any 3mm
} } prepared grid
} } for use as a TEM mag std traceable to NIST. I am performing
} } magnification
} } checks from 1,000X through 200,000X. Any info would be much
} } appreciated.
} }
} }
} } David Cugier
} } Assistant Scientist
} } Microscopy and Microanalysis
} } Abbott Laboratories
} } Abbott Park, Illinois 60064
} } 847-938-6725
} }




From daemon Tue Jul 11 12:20:24 2000



From: Mary Gail Engle :      mgengle-at-pop.uky.edu
Date: Tue, 11 Jul 2000 13:03:35 -0400
Subject: flat embedding molds

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Do any of you have problems getting resin blocks to release from the flat,
blue embedding molds? We have used these for years with no trouble but the
newer molds don't let the blocks go after just a few embeddings. I have
had to cut them out with a razor blade which of course ruins the mold. Has
the formula for the molds changed or is it the resin? We use Spurr's and
Eponate 12 primarily.

Thank you,
Mary Gail Engle
Electron Microscopy & Imaging Facility
University of Kentucky Medical Center




From daemon Tue Jul 11 12:46:27 2000



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Tue, 11 Jul 2000 13:38:17 -0500
Subject: TEM calibration

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

David Cugier wrote:
==============================================================
I am currently searching for a NIST traceable TEM mag. standard. The reason
for a NIST traceable standard is that our EM facility is in transition to
becoming GMP regulated. I have used the waffle grating replica and catalase
crystals in the past and prefer this type of standard but they are not NIST
traceable. I am aware that NIST offers polystyrene nanosphere particles for
sizing but was hoping to avoid using because of the difficulty in producing
a uniform monolayer without aggregate particles. I am also aware that there
is a product called MAGICAL and am still waiting from a response from NIST
as to whether or not it is traceable. Does anyone know of any 3mm prepared
grid for use as a TEM mag std traceable to NIST. I am performing
magnification checks from 1,000X through 200,000X. Any info would be much
appreciated.
===============================================================
This is a really tough question to answer. The Mag*I*Cal TEM calibration
standard comes "close" but "close" in this business is not quite enough.
But it might be the best there is. You can get information about the
Mag*I*Cal at URL
http://www.2spi.com/catalog/standards/magical.html

You are correct about the problems associated with using the polystryene
calibrated spheres.

It is sold by SPI Supplies as well as some of the other major suppliers of
accessories and consumables for EM laboratories.

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.spi.cc
########################
============================================









From daemon Tue Jul 11 13:11:00 2000



From: Tom Phillips :      PhillipsT-at-missouri.edu
Date: Tue, 11 Jul 2000 12:44:49 -0500
Subject: Re: flat embedding molds

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


This exactly mirrors the problem I have noticed in the last couple of
years. My blue molds formerly lasted for years and now they
deteriorate rapidly. We use an epon 812 style embedding medium. I
have taken to spraying the molds with teflon spray (708 T.F.E. dry
lube - a lot cheaper from the local hardware store than from EM
supply houses!) and that seems to help without hurting morphology.
but i can't help but wonder what has changed in molds!






}
}
} Do any of you have problems getting resin blocks to release from the
} flat, blue embedding molds? We have used these for years with no
} trouble but the newer molds don't let the blocks go after just a few
} embeddings. I have had to cut them out with a razor blade which of
} course ruins the mold. Has the formula for the molds changed or is
} it the resin? We use Spurr's and Eponate 12 primarily.
}
} Thank you,
} Mary Gail Engle
} Electron Microscopy & Imaging Facility
} University of Kentucky Medical Center

Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)



From daemon Tue Jul 11 14:00:13 2000



From: McCaffrey, John :      John.McCaffrey-at-nrc.ca
Date: Tue, 11 Jul 2000 14:48:44 -0400
Subject: TEM calibration

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Chuck,

It might have been a bit more helpful if you had suggested that
users should contact NIST and request that they evaluate the MAG*I*CAL,
since the industry now requires a traceable standard.

Cheers
John


John P. McCaffrey, Ph.D.
National Research Council of Canada
M-50, Montreal Rd.
Ottawa, Ontario
K1A 0R6 CANADA

tel: +613-993-7823
fax: +613-990-0202
email: john.mccaffrey-at-nrc.ca


-----Original Message-----
} From: Garber, Charles A. [mailto:cgarber-at-2spi.com]
Sent: Tuesday, July 11, 2000 2:38 PM
To: MICROSCOPY BB


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

David Cugier wrote:
==============================================================
I am currently searching for a NIST traceable TEM mag. standard. The reason
for a NIST traceable standard is that our EM facility is in transition to
becoming GMP regulated. I have used the waffle grating replica and catalase
crystals in the past and prefer this type of standard but they are not NIST
traceable. I am aware that NIST offers polystyrene nanosphere particles for
sizing but was hoping to avoid using because of the difficulty in producing
a uniform monolayer without aggregate particles. I am also aware that there
is a product called MAGICAL and am still waiting from a response from NIST
as to whether or not it is traceable. Does anyone know of any 3mm prepared
grid for use as a TEM mag std traceable to NIST. I am performing
magnification checks from 1,000X through 200,000X. Any info would be much
appreciated.
===============================================================
This is a really tough question to answer. The Mag*I*Cal TEM calibration
standard comes "close" but "close" in this business is not quite enough.
But it might be the best there is. You can get information about the
Mag*I*Cal at URL
http://www.2spi.com/catalog/standards/magical.html

You are correct about the problems associated with using the polystryene
calibrated spheres.

It is sold by SPI Supplies as well as some of the other major suppliers of
accessories and consumables for EM laboratories.

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.spi.cc
########################
============================================









From daemon Tue Jul 11 16:23:12 2000



From: Michael Parrish :      t80mjp1-at-wpo.cso.niu.edu
Date: Tue, 11 Jul 2000 15:50:28 -0500
Subject: Research Associate Position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The Department of Biological Sciences at Northern Illinois University
has an immediate opening for a Research Associate in Confocal/Electron
Microscopy. Details are in the advertisement below.

Thanks

Michael Parrish, Professor and Chair
Biological Sciences
Northern Illinois University
DeKalb, IL 60115
815-753-1753

Research Associate:

The Department of Biological Sciences at Northern Illinois University
has an opening for a Research Associate with expertise in Confocal and
Electron Microscopy. The successful candidate will be expected to
operate our confocal, transmission and scanning electron microscopes and
to supervise both students and faculty in their use. Master's degree in
Biology or related field required including at least two years'
experience using confocal and electron microscopes in biological or
chemical research. Ideally, the successful candidate should be able to
calibrate and perform routine maintenance on the instruments. Submit
letter of interest, resume and three letters of recommendation to: Dr.
Michael Parrish, Chair, Department of Biological Sciences, Northern
Illinois University, DeKalb, IL 60115-2861; http:\\www.bios.niu.edu;
phone: 815-753-0461; email: biosjobs-at-niu.edu. Review of applications
will begin July 28, 2000 and continue until position is filled.



From daemon Tue Jul 11 16:47:25 2000



From: Dohnalkova, Alice :      Alice.Dohnalkova-at-pnl.gov
Date: Tue, 11 Jul 2000 14:38:13 -0700
Subject: JEOL 2010 Cold Trap

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As a relative novice on JEOL 2010 TEM, I was recently advised to use a cold trap
in the specimen area. Since I know some people don't use it even though it's
mounted on their 'scope, I'd like to hear your opinion on when and how
frequently to use it. I understand it improves the vacuum significantly; is
there any drawback? Any guidelines or suggestions on the liquid N2 /heat-up
cycle? Thank you, Alice.

Alice Dohnalkova
Environmental Microbiology
Battelle, PNNL
Richland, WA
(509) 372-0692




From daemon Tue Jul 11 20:05:45 2000



From: Bruce Brinson :      brinson-at-cnst.rice.edu
Date: Tue, 11 Jul 2000 19:59:31 -0500
Subject: Re: JEOL 2010 Cold Trap

Contents Retrieved from Microscopy Listserver Archives
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HI Alice,
Use the cold trap. Use the cold trap. Did I say use the cold trap? Jeol calls it
the anti contamination device (ACD). Your vacuum will be better, sample swap time
quicker, much quicker. The cold trap will pump (trap) most emissions from your
sample. This is a good thing. It keeps them from carbonizing on your apertures or
oxidizing the filament. Ever noticed really strange images of your filament that
eventually went away? Better vacuum will extend your filament life.
I find it hard to believe (I believe you) that users in your facility are not
using the ACD. I could rant on a bit longer....
There is one catch, a testament to it's pumping efficiency. When you are
finished, you must use the ADC heat cycle. The ACD will have accumulated so many
molecules that the ion pump can not handle the gas load if the ACD warms up on it's
own. The ACD heat cycle will switch the column over to the diffusion pump for a
period of time. In our lab, the last user signed up (weather or not they use the
TEM) is responsible for tending to the ACD.
In our lab, failure to use the ACD heat cycle is a high crime. It creates a
vacuum condition (in our 2010) that causes the gun isolation valve to cycle at ~0.5
Hz. This then causes the bellows to fail, typically in ~10,000 cycles (a long
weekend). The effect is cumulative.
So now that I said use it. how... fill the dewier, wait ~5min & top it off. Put
a foil cap or other loose fitting cap over the fill port. It should stay cold for 7
hours. That is the Spec. on our unit. It varies a bit with # of sample changes. I
have seen it cold 11 hours later. Don't ask how I know this :)

Bruce Brinson
Rice U.

"Dohnalkova, Alice" wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} As a relative novice on JEOL 2010 TEM, I was recently advised to use a cold trap
} in the specimen area. Since I know some people don't use it even though it's
} mounted on their 'scope, I'd like to hear your opinion on when and how
} frequently to use it. I understand it improves the vacuum significantly; is
} there any drawback? Any guidelines or suggestions on the liquid N2 /heat-up
} cycle? Thank you, Alice.
}
} Alice Dohnalkova
} Environmental Microbiology
} Battelle, PNNL
} Richland, WA
} (509) 372-0692




From daemon Wed Jul 12 01:27:57 2000



From: Ken Tiekotter :      tiekotte-at-up.edu
Date: Tue, 11 Jul 2000 23:16:16 -0700 (PDT)
Subject: Re: JEOL 2010 Cold Trap

Contents Retrieved from Microscopy Listserver Archives
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Dear Alice,

I routinely use a combination of LN2 and ion getter pumps on my Zeiss EM
10. The scope is also kept running 24 hours a day, 7 days a week. This
combination has afforded me with extremely good vacuum, less downtime, and
an incredible life time on tungsten filaments. The filament I am currently
using has 430 hours: my record is 520 hours! This of course is also
relately to slow saturation and desaturation of the filament whenever I
turn off or on the high tension, completely outgassing of film after film
exchange and using the scope with liquid nitrogen.

The only disadvantage occurs when the LN2 is depleted and the cold finger
around the specimen starts to return to ambient temperature, i.e., there
is a substantial rise in vacuum pressure until the cold finger warms
up. The advantages I have experienced include long filament life, clean
high vacuum, and no contamination on the grid/specimen. I routinely use
the scope at magnifications from x1600 to x125,000 and have not notice
degradation of image quality, either from hydrocarbon contamination or an
aged filament.

I pay about $47.00 for 50 liters of LN2 every 5 to 7 weeks: worth every
cent!! One must however get into the habit of using filling the scope
dewar about 20 to 30 minutes before using the scope.

Sincerely,
Ken
-------
Ken Tiekotter, Adjunct Professor
The University of Portland
Dept. of Biology
5000 N. Willamette Blvd.
Portland, OR 97203

Director, MicroImaging/Electron Micrscopy, G50
Legacy Holladay Park Medical Center
1225 NE 2nd Avenue
Portland, OR 97232

TEL.: (503) 413-5391

On Tue, 11 Jul 2000, Dohnalkova, Alice wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} As a relative novice on JEOL 2010 TEM, I was recently advised to use a cold trap
} in the specimen area. Since I know some people don't use it even though it's
} mounted on their 'scope, I'd like to hear your opinion on when and how
} frequently to use it. I understand it improves the vacuum significantly; is
} there any drawback? Any guidelines or suggestions on the liquid N2 /heat-up
} cycle? Thank you, Alice.
}
} Alice Dohnalkova
} Environmental Microbiology
} Battelle, PNNL
} Richland, WA
} (509) 372-0692
}
}
}




From daemon Wed Jul 12 02:27:42 2000



From: Dr Malcolm Roberts :      m.roberts-at-ru.ac.za
Date: Wed, 12 Jul 2000 09:16:46 +0200
Subject: Ultra thin Moxtex windows

Contents Retrieved from Microscopy Listserver Archives
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Hallo Folks!
This is a reply to a previous posting of mine regarding a spurious B
peak in carbonate spectra plus another matter altogether. Firstly, for
Warren S's info, we have a refurbished Kevex detector with a Si (Li)
crystal and an ultra thin Moxtex window (3 microns) attached to our JEOL
733. The pulse processing electronics are 1976 vintage Kevex 4050 which
give us a pitiful ca. 1200 cps at 25% dead time. C peak occurs in the
correct place. To answer Brendan G., the oxygen peak is in the correct
place, and everything seems to be in order apart from the spurious peak.
Basically, the system is calibrated to give the correct things in the
correct place.
Now to another problem, and this has to do with the window itself.
We have been watching the Cu l:k alpha ratio deteriorate over time
since installation 4 months ago from acceptable values over 1 to an
alarming 0.44 (intergrated peak ratio specified within WinEDS software).
Micro droplets of oil are starting to appear on other wise clean
standards (although these are few and dispersed rather than a slick, and
I am not alarmed by this considering all other things. I'm actually
happy after 1.5 years to be able to analyse things!!), and I suspect
that the window is in need of a wash off. The company who outsourced the
refurbishment, chose the window, and installed the system have told me
that ultra-thin Moxtex windows cannot be cleaned. If this is true, then
we have been stitched up. Does anyone out there know anything about
these things? Some advice, as usual, very helpful to us L-drivers.
Cheers,
Malc.

--
Dr MP Roberts Phone: [+27](0)46 603 8313
Dept of Geology Fax: [+27](0)46 622 9715
Rhodes University Cell: 083 4060 262 (usually off)
6140 Grahamstown e-mail: m.roberts-at-ru.ac.za
SOUTH AFRICA





From daemon Wed Jul 12 02:49:36 2000



From: Ron Doole :      ron.doole-at-materials.oxford.ac.uk
Date: Wed, 12 Jul 2000 08:40:58 +0100 (BST)
Subject: Re: JEOL 2010 Cold Trap

Contents Retrieved from Microscopy Listserver Archives
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Hi Alice,

Bruce has got it right - use the ACD and heat it up overnight. Any cold
trap will help remove hydrocarbons and water vapour from your
vacuum system.

A few of extra notes.
- Fill the trap as soon as you get to the instrument to get it cold. It
can be cooling down while you ramp up the HT and load a specimen.
- Beware that some dewars throw out a quantity of gas and sometimes liguid
N2 shortly after they have been filled from room temperature. Do not sit
under the dewar for 5 minutes after first filling.
- After you hit the ACD heat button the vacuum system locks up for about 2
hours while the ACD warms up and the trapped gasses are pumped away by the
diff pump. Remember to change films and specimen and let them pump down
properly before hitting the ACD button.

I am at a materials lab rather than a biological one, our users need the
2010 for small probe work and that shows up any contamination problem. A
routine of column bake out and use of the ACD means that I can be sure
that contamination problems come from their specimen.

Regards,
Ron

On Tue, 11 Jul 2000, Dohnalkova, Alice wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} As a relative novice on JEOL 2010 TEM, I was recently advised to use a cold trap
} in the specimen area. Since I know some people don't use it even though it's
} mounted on their 'scope, I'd like to hear your opinion on when and how
} frequently to use it. I understand it improves the vacuum significantly; is
} there any drawback? Any guidelines or suggestions on the liquid N2 /heat-up
} cycle? Thank you, Alice.
}
} Alice Dohnalkova
} Environmental Microbiology
} Battelle, PNNL
} Richland, WA
} (509) 372-0692
}
}
}

===========================================================================
Mr. Ron Doole e-mail ron.doole-at-materials.ox.ac.uk
Department of Materials, phone +44 (0) 1865 273701
University of Oxford, fax +44 (0) 1865 283333
Parks Road.
Oxford. OX1 3PH. UK.
============================================================================




From daemon Wed Jul 12 06:40:47 2000



From: Chris Jeffree :      cjeffree-at-srv0.bio.ed.ac.uk
Date: Wed, 12 Jul 2000 12:29:24 +0000
Subject: Cold traps and cold fingers in SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


While we are on a slight cold trap / ACD thread, I would greatly
appreciate your views about the desirability of using cold traps and
cold fingers in Field emission SEMs. Some manufacturers have
them, others don't and regard them as unnecessary. Some
manufacturers exchange specimens by opening the chamber to
air, others do it via airlocks. If one is aiming to maximise the
performance of a FESEM for low-kV and LTSEM imaging it would
seem to me to be desirable not to expose the specimen chamber
to atmospheric pressure during specimen changes, and to trap any
volatiles to reduce specimen contamination, just as we would
normally do in TEM practice. Do users of FESEMs find in practice
that there are specimen contamination issues which make the use
of cryo-trapping and airlocks desirable, or are these features
unnecessary?

Best wishes
Chris
=====================================================================
DR CHRIS JEFFREE
BIOSEM - BIOLOGICAL SCIENCES EM FACILITY
UNIVERSITY OF EDINBURGH
Daniel Rutherford Building
King's Buildings, Mayfield Road
EDINBURGH, EH9 3JH, Scotland, UK
Tel. #44 131 650 5345
FAX. #44 131 650 6563
Mobile 07710 585 401
email c.jeffree-at-ed.ac.uk
SEM / TEM bookings sem-at-ed.ac.uk
=====================================================================



From daemon Wed Jul 12 08:38:51 2000



From: Fred Pearson :      eoptics-at-mcmail.cis.mcmaster.ca
Date: Wed, 12 Jul 2000 09:29:17 -0400 (EDT)
Subject: Looking for Electopolishing recipe

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Good day to all:

I am sending this request for a researcher who is not a memeber of the
list so kindly respond to her (Aleksandra Perovic) directly at:

perovic-at-mcmaster.ca

We want to electropolish this alloy which contains the following phases.

1) Q phase: 30 wt% Mg
30 wt% Si
16 wt% Al
& 20 wt% Cu.

2) Theta phase: Al2Cu

3) Si:

4) Beta phase: Mg2Si

We have already tried ion milling and also electropolishing with 10%
perchloric/methanol, with no success.

If anyone has a recipe for such an alloy we would appreciate hearing
from you.

Thanks in advance


Fred

c/o Aleksandra Perovic

********************************************************
Fred Pearson
Brockhouse Institute for Materials Research
McMaster University
1280 Main St. West
Hamilton, Ontario
Canada L8S 4M1


email: eoptics-at-mcmaster.ca
phone: (905) 525-9140 ext. 24609
fax: (905) 521-2773
********************************************************





From daemon Wed Jul 12 08:42:06 2000



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Wed, 12 Jul 2000 08:31:04 -0500
Subject: Cold traps and cold fingers in SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Chris,

We have an Hitachi S4700 FESEM, and we routinely use the LN2 coldfinger. In
my experience, it reduces deposition of contamination under the beam,
especially during low voltage operation (1-5kV, say).

The disadvantages so far have been vibration introduced by the boiling LN2,
although this pretty much disappears after about 20-30 minutes, and the
problem of retrieving a sample which has become dislodged inside the
chamber. Although the latter occurrence is rare, it obviously requires
opening up the entire chamber to atmosphere, which isn't a great thing to do
with a chilly cold finger combined with the humidity of mid-Missouri. In
this case, you need to wait until the system comes to room temperature,
effectively shutting down the scope for several hours.

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/




-----Original Message-----
} From: Chris Jeffree [mailto:cjeffree-at-srv0.bio.ed.ac.uk]
Sent: Wednesday, July 12, 2000 7:29 AM
To: microscopy-at-sparc5.microscopy.com


While we are on a slight cold trap / ACD thread, I would greatly
appreciate your views about the desirability of using cold traps and
cold fingers in Field emission SEMs. Some manufacturers have
them, others don't and regard them as unnecessary. Some
manufacturers exchange specimens by opening the chamber to
air, others do it via airlocks. If one is aiming to maximise the
performance of a FESEM for low-kV and LTSEM imaging it would
seem to me to be desirable not to expose the specimen chamber
to atmospheric pressure during specimen changes, and to trap any
volatiles to reduce specimen contamination, just as we would
normally do in TEM practice. Do users of FESEMs find in practice
that there are specimen contamination issues which make the use
of cryo-trapping and airlocks desirable, or are these features
unnecessary?

Best wishes
Chris
=====================================================================
DR CHRIS JEFFREE
BIOSEM - BIOLOGICAL SCIENCES EM FACILITY
UNIVERSITY OF EDINBURGH
Daniel Rutherford Building
King's Buildings, Mayfield Road
EDINBURGH, EH9 3JH, Scotland, UK
Tel. #44 131 650 5345
FAX. #44 131 650 6563
Mobile 07710 585 401
email c.jeffree-at-ed.ac.uk
SEM / TEM bookings sem-at-ed.ac.uk
=====================================================================



From daemon Wed Jul 12 08:49:31 2000



From: rlvaughn-at-unmc.edu
Date: Wed, 12 Jul 2000 08:41:58 -0500
Subject: Re: flat embedding molds

Contents Retrieved from Microscopy Listserver Archives
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Dito here. The vendor asked if I was embedding at high temps but we use
araldite or 812 at 60 - 65 degree. We sometimes sprayed the molds with
high purity silicone which would extend the life after they started
sticking. The clear ones also got hard and opaque, along with ambrose
silicone molds.

Rick Vaughn




From daemon Wed Jul 12 09:24:16 2000



From: jim :      jim-at-proscitech.com.au
Date: Wed, 12 Jul 2000 23:20:46 +1000
Subject: want to know national permit requirements for Uranyl Acetate

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The Australian, and now the various State Governments require permits for the
import, storage and transshipping of UA (also a store and radiation safety
officer). We will in future drop-ship to the end user direct and therefore not
handle UA at all. The crazy rules are laid bare in page, which is linked from
our UA item online.

I would like to know from international subscribers (can be back-channel) of
any permits required to import or transship UA in other countries. I know that
UA can be shipped by normal air freight and through the Postal system anywhere.
The USA definition of a radioactive substance is 7x higher than the Australian
one. The new legislation will cost Australian labs a good deal of extra time
and money. It would be useful to protest to the various Health ministers, State
and Federal.

I would also like to know of any significant incident involving UA anywhere. I
should point out that radiation damage would not show for years and one would
never know the original cause. The radiation though is low, so unless a person
would take to sleeping with a jar of UA there is no real danger. The real
danger of UA is as a non-cumulative (happily), but severe kidney poison, but
this is of course no issue with the Radiation people.
Cheers
Jim Darley

ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com




From daemon Wed Jul 12 10:37:29 2000



From: Drouillon, Philippe :      Philippe.Drouillon-at-solvay.com
Date: Wed, 12 Jul 2000 17:24:04 +0200
Subject: HELP WANTED

Contents Retrieved from Microscopy Listserver Archives
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Hello,

The P2 projector lens of our TEM Siemens Elmiskop 101 is out of order.

We need to replace it as soon as possible.

Could anyone help us ?

Here are the features of our microscope :

Siemens Elmiskop 101 - Fabrication number : 2368
Serial number : M31191-A5
High voltage working between 40 and 100 kV - 60 µA

Thanks in advance for your help

Best regards

Philippe Drouillon
Solvay Research and Technology
Analytical Technologies Dpt
Electron Microscopy and Image Analysis Labs - IT coordinator of the Dpt
Rue de Ransbeek, 310
B-1120 Brussels (Belgium)
phone : (00 32) 2 264 24 47
mailto:philippe.drouillon-at-solvay.com


} -----Original Message-----
} From: Bruce Brinson [SMTP:brinson-at-cnst.rice.edu]
} Sent: Wednesday, July 12, 2000 3:00 AM
} To: Dohnalkova, Alice
} Cc: 'Microscopy-at-MSA.Microscopy.com'
} Subject: Re: JEOL 2010 Cold Trap
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} HI Alice,
} Use the cold trap. Use the cold trap. Did I say use the cold trap?
} Jeol calls it
} the anti contamination device (ACD). Your vacuum will be better, sample
} swap time
} quicker, much quicker. The cold trap will pump (trap) most emissions from
} your
} sample. This is a good thing. It keeps them from carbonizing on your
} apertures or
} oxidizing the filament. Ever noticed really strange images of your
} filament that
} eventually went away? Better vacuum will extend your filament life.
} I find it hard to believe (I believe you) that users in your facility
} are not
} using the ACD. I could rant on a bit longer....
} There is one catch, a testament to it's pumping efficiency. When you
} are
} finished, you must use the ADC heat cycle. The ACD will have accumulated
} so many
} molecules that the ion pump can not handle the gas load if the ACD warms
} up on it's
} own. The ACD heat cycle will switch the column over to the diffusion pump
} for a
} period of time. In our lab, the last user signed up (weather or not they
} use the
} TEM) is responsible for tending to the ACD.
} In our lab, failure to use the ACD heat cycle is a high crime. It
} creates a
} vacuum condition (in our 2010) that causes the gun isolation valve to
} cycle at ~0.5
} Hz. This then causes the bellows to fail, typically in ~10,000 cycles (a
} long
} weekend). The effect is cumulative.
} So now that I said use it. how... fill the dewier, wait ~5min & top
} it off. Put
} a foil cap or other loose fitting cap over the fill port. It should stay
} cold for 7
} hours. That is the Spec. on our unit. It varies a bit with # of sample
} changes. I
} have seen it cold 11 hours later. Don't ask how I know this :)
}
} Bruce Brinson
} Rice U.
}
} "Dohnalkova, Alice" wrote:
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } As a relative novice on JEOL 2010 TEM, I was recently advised to use a
} cold trap
} } in the specimen area. Since I know some people don't use it even though
} it's
} } mounted on their 'scope, I'd like to hear your opinion on when and how
} } frequently to use it. I understand it improves the vacuum
} significantly; is
} } there any drawback? Any guidelines or suggestions on the liquid N2
} /heat-up
} } cycle? Thank you, Alice.
} }
} } Alice Dohnalkova
} } Environmental Microbiology
} } Battelle, PNNL
} } Richland, WA
} } (509) 372-0692
}



From daemon Wed Jul 12 11:07:11 2000



From: Bryan Tracy :      bryant-at-brahms.amd.com
Date: Wed, 12 Jul 2000 08:56:35 -0700
Subject: Looking for TEM holder for a Topcon 002B

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello EM'ers,

We are very much interested to purchase an extra
Gatan Double Tilt holder for our Topcon 002B TEM.

We have the 1.9A polepiece (EM-P20).

If you have any leads, please contact me

Thanks so much for your help,


Bryan Tracy
Advanced Micro Devices, Sunnyvale, ca
bryant-at-brahms.amd.com
408-749-4819

Bryan Tracy
Materials Technology Development
Advanced Micro Devices
915 Deguigne Ave, Mailstop 143
Sunnyvale, Ca, 94088





From daemon Wed Jul 12 11:59:47 2000



From: Yan Xin :      xin-at-magnet.fsu.edu
Date: Wed, 12 Jul 2000 12:49:50 -0400
Subject: JEOL 2010 Cold Trap

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


It seems that it is a good idea to use a cold trap on JEOL 2010 TEM. But I
have a concern if anyone has any suggestions. We have a LaB6 JEOL
2010. Once the ACD is hit, the HT is automatically drops down to zero. So
for the next day, the HT has to be ramped up again. If we do like this,
the HT will have to be ramped up everyday, and I am wondering whether this
will do any harm in a long run.
Thanks.
Regards
Yan Xin


=======================================
Yan Xin
Magnet Science & Technology
National High Magnetic Field Laboratory
Florida State University
1800 E. Paul Dirac Drive
Tallahassee, FL 32310
Tel: (850) 644 1529
Fax: (850) 644 0867
========================================






From daemon Wed Jul 12 13:53:25 2000



From: Becky Holdford :      r-holdford-at-ti.com
Date: Wed, 12 Jul 2000 13:50:12 -0500
Subject: Re: Cold traps and cold fingers in SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Randy and Listies:
I too have an S4700 with a cold finger. But I don't have the time to let the
cold finger come to room temperature if I need to retrieve a dropped or stuck
sample. Plant A/C keeps the lab humidity below 60% and if I work fast, there is
minimal frost buildup. The main chamber is back under working vacuum in less
than 1 hour. The scope is equipped with a diffusion pump instead of a TMP. My
question: Am I causing any problems by letting the pump system sublime the
frost off the cold finger?

"Tindall, Randy D." wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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} -----------------------------------------------------------------------.
}
} Chris,
}
} We have an Hitachi S4700 FESEM, and we routinely use the LN2 coldfinger. In
} my experience, it reduces deposition of contamination under the beam,
} especially during low voltage operation (1-5kV, say).
}
} The disadvantages so far have been vibration introduced by the boiling LN2,
} although this pretty much disappears after about 20-30 minutes, and the
} problem of retrieving a sample which has become dislodged inside the
} chamber. Although the latter occurrence is rare, it obviously requires
} opening up the entire chamber to atmosphere, which isn't a great thing to do
} with a chilly cold finger combined with the humidity of mid-Missouri. In
} this case, you need to wait until the system comes to room temperature,
} effectively shutting down the scope for several hours.
}
} Randy
}
} Randy Tindall
} EM Specialist
} Electron Microscopy Core Facility
} W122 Veterinary Medicine
} University of Missouri
} Columbia, MO 65211
} Tel: (573) 882-8304
} Fax: (573) 884-5414
} Email: tindallr-at-missouri.edu
} Web: http://www.biotech.missouri.edu/emc/
}

--
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Becky Holdford (r-holdford-at-ti.com)
972-598-1291 (pager)
KFAB Physical Analysis Lab--SEM/FIB/FA
Texas Instruments, Inc.
Dallas, TX
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~





From daemon Wed Jul 12 14:41:06 2000



From: Chuck Buiocchi :      buiocchi-at-astro.ocis.temple.edu
Date: Wed, 12 Jul 2000 15:30:35 -0400
Subject: Electron Microscopes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All,
The Anatomy / Cell Biology Department of TEMPLE UNIVERSITY / MED SCHOOL,
has three (3) Electron Microscopes it would like to Donate to a good home,
for the cost of removing the item you would like. The three are as follows:

1. Philips 300 TEM.

2. JEOL 100B TEM.

3. ETEC CORP. AUTOSCAN SEM.

If you have a need for one or all three microscopes, Please contact
Dr. Joanne Orth by E-mail (ASAP) -at- "orthjo-at-astro.temple.edu".
The Philips and ETEC were working well a couple of years ago, I have no
knowledge of the JEOL.

Thank You,
Chuck Buiocchi, Lab Mgr., Anatomy/Cell Biology




From daemon Wed Jul 12 18:27:19 2000



From: Don Chernoff at ASM :      donc-at-asmicro.com
Date: Wed, 12 Jul 2000 18:16:39 -0500
Subject: MSA meeting: Caution about online Registration

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Microscopy and Microanalysis 2000 is August 13-17 in Philadelphia.
Today I received an email reminder to register:
} Register at www.peregrine.net/mm2000
When I went to this site, the URL prefix was http:, not https:
The latter would indicate a secure server.
The text of the web page claimed that it is secure, but Netscape warned that
it is not.
Since this site is not secure, despite its claim, I did not submit my credit
card information online.
I advise other registrants to be cautious also.

Don Chernoff

} Questions? Please refer to the MSA web site at
} www.msa.microscopy.com
} or contact Meeting Management at (708) 361-6045.


Advanced Surface Microscopy, Inc. E-Mail: donc-at-asmicro.com
6009 KNYGHTON RD. Voice: 317-251-1364
INDIANAPOLIS IN 46220 Toll free: 800-374-8557 (in USA)
web: http://www.asmicro.com Fax: 317-254-8690
Please use the new web and email addresses shown above.
(February 1999)



From daemon Wed Jul 12 22:04:44 2000



From: Ronald Vane :      RVaneXEI-at-concentric.net
Date: Wednesday, July 12, 2000 5:58 AM
Subject: Cold traps and cold fingers in SEM

Contents Retrieved from Microscopy Listserver Archives
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Here are my observations about cold fingers and cold traps in SEMs. They do
a good job of fighting contamination but they do have the vibration and
frosting problems noted by others. They require constant refilling to keep
them cold. When they warm up the water and oil they collect mostly
redistributes to the walls of the chamber and is not pumped away. After
using a cold finger cold, the warm cold finger is a major source of
contamination.

If a cold finger is frosted during opening a door for dislodging a sample,
then pumping the frost should not overburden the vacuum pumps if done
occasionally. They are designed to handle the water vapor in the atmosphere.
Excessive water vapor pumping may condense water in rotary pump oil. More
frequent oil changes are recommended if water vapor is pumped regularly.

I believe that some manufacturers claim cold traps are not needed because of
cost and competitive issues. They are selling the merits of their dry
pumping systems. However the vacuum system is not the only oil source for
contamination. The specimens themselves unless they are freshly cleaned will
carry a hydrocarbon scum into the chamber. Cold traps are effective for this
problem even on dry pumped systems. Contamination is always observed even in
the cleanest, dry-pumped SEM if long scans are used. Cold traps and cold
fingers are some of the tools available to control the problem.

Notice: XEI Scientific makes anti-contamination systems for SEMs but not
cold traps or cold fingers. See www.SEMCLEAN.com for information.

Ronald Vane
XEI Scientific
650-369-0133




-----Original Message-----
} From: Chris Jeffree {cjeffree-at-srv0.bio.ed.ac.uk}
To: microscopy-at-sparc5.microscopy.com {microscopy-at-sparc5.microscopy.com}




From daemon Thu Jul 13 00:01:31 2000



From: jim :      jim-at-proscitech.com.au
Date: Thu, 13 Jul 2000 11:26:16 +1000
Subject: RE: Cold traps and cold fingers in SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Not a good idea to have the pumps loaded up with so much water. It does not
take long to warm the cold finger: A beaker with boiling water on the external
finger will rise the internals of the trap within 5 minutes to above room
temperature.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Thursday, July 13, 2000 4:50 AM, Becky Holdford [SMTP:r-holdford-at-ti.com]
wrote:
}
}
} Randy and Listies:
} I too have an S4700 with a cold finger. But I don't have the time to let the
} cold finger come to room temperature if I need to retrieve a dropped or stuck
} sample. Plant A/C keeps the lab humidity below 60% and if I work fast, there
} is
} minimal frost buildup. The main chamber is back under working vacuum in less
} than 1 hour. The scope is equipped with a diffusion pump instead of a TMP.
} My
} question: Am I causing any problems by letting the pump system sublime the
} frost off the cold finger?
}
} "Tindall, Randy D." wrote:
}
}
} }
} } Chris,
} }
} } We have an Hitachi S4700 FESEM, and we routinely use the LN2 coldfinger.
} } In
} } my experience, it reduces deposition of contamination under the beam,
} } especially during low voltage operation (1-5kV, say).
} }
} } The disadvantages so far have been vibration introduced by the boiling LN2,
} } although this pretty much disappears after about 20-30 minutes, and the
} } problem of retrieving a sample which has become dislodged inside the
} } chamber. Although the latter occurrence is rare, it obviously requires
} } opening up the entire chamber to atmosphere, which isn't a great thing to
} } do
} } with a chilly cold finger combined with the humidity of mid-Missouri. In
} } this case, you need to wait until the system comes to room temperature,
} } effectively shutting down the scope for several hours.
} }
} } Randy
} }
} } Randy Tindall
} } EM Specialist
} } Electron Microscopy Core Facility
} } W122 Veterinary Medicine
} } University of Missouri
} } Columbia, MO 65211
} } Tel: (573) 882-8304
} } Fax: (573) 884-5414
} } Email: tindallr-at-missouri.edu
} } Web: http://www.biotech.missouri.edu/emc/
} }
}
} --
} ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
} Becky Holdford (r-holdford-at-ti.com)
} 972-598-1291 (pager)
} KFAB Physical Analysis Lab--SEM/FIB/FA
} Texas Instruments, Inc.
} Dallas, TX
} ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
}
}




From daemon Thu Jul 13 00:26:01 2000



From: Harold A. Brink :      hbrink-at-gatan.com
Date: Wed, 12 Jul 2000 22:14:42 -0700
Subject: TEM Job opening at Gatan Inc.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html





Job opening at Gatan Inc.:

Systems Engineer, Analytical TEM


Gatan’s Inc. is currently looking for an enthusiastic and highly organized
person to help maintain and develop our line of state-of-the-art Analytical
TEM products.

In this position you will be responsible for maintaining and further
improving the overall, system level, performance of our existing TEM
instrumentation. You will work extensively with our mechanical, electrical
and software engineering groups, as well as our manufacturing and service
departments. Experience in these fields, and strong communication skills,
are therefore a must.

This is a key position within our organization and will involve some
national and international travel. You will be based in Pleasanton,
California. Practical TEM experience will be considered as a strong plus.

Gatan offers an exciting, high technology environment within a small, but
growing organization.

Interested parties, please submit your resume and salary requirements to
Harold A. Brink at hbrink-at-gatan.com.




From daemon Thu Jul 13 04:04:33 2000



From: richard.beanland-at-gecm.com
Date: Thu, 13 Jul 2000 10:28:26 +0000 (GMT)
Subject: Surface Science Journal - being thrown away

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


True. So this could be an argument for using turbomolecular
pumps, which are tolerant of water vapour. Is it advisable to subject
the cold finger dewar to thermal shock by putting boiling water into
it?
Chris

} From: jim {jim-at-proscitech.com.au}
Send reply to: "jim-at-proscitech.com.au" {jim-at-proscitech.com.au}
To: "'Becky Holdford'" {r-holdford-at-ti.com} ,
Microscopy ListServer
{microscopy-at-sparc5.microscopy.com}


Dear all,
I have a set of the Journal 'Surface Science' and 'Surface Science reports', 1990-1993 (inclusive). If anyone wants it, please get in touch with me by 20th of this month or it will go in the skip.

Best regards,

Richard Beanland

==============================================================
Richard Beanland
Caswell Technology,
Caswell,
Towcester,
Northants NN12 8EQ

e-mail richard.beanland-at-gecm.com
Tel. +44 1327 356363
Fax. +44 1327 356398
==============================================================
"The information contained in this message is legally privileged and confidential information intended for the eyes of the individual or entity named above. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message is strictly prohibited. If you have received this message in error, please notify us immediately by telephone.
Caswell Technology is the trading name of Marconi Caswell Limited. Registered in London No. 3694360 Registered Office: One Bruton Street London W1X 8AQ. Holding Company: Marconi plc."







From daemon Thu Jul 13 06:49:18 2000



From: Marti, Jordi :      jordi.marti-at-honeywell.com
Date: Thu, 13 Jul 2000 04:38:46 -0700
Subject: EBSD ?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Could somebody briefly educate me on EBSD (Electron Backscattered ...?) . I
am trying to find information for a friend. He would like to know the very
basics, capabilities of the technique (for example, resolution) and if there
are contract labs that do this. Since he is interested in quantitative
texture analysis, crystal orientation and GB analysis I assume that he is
talking about getting and analyzing channeling patterns, but I could be
wrong. I did not get from him what materials he is interested in. So, any
information on EBSD would be appreciated.

Thanks

Jordi Marti
Honeywell



From daemon Thu Jul 13 07:17:32 2000



From: jim :      jim-at-proscitech.com.au
Date: Thu, 13 Jul 2000 22:02:14 +1000
Subject: RE: Cold traps and cold fingers in SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


That is why I wrote "beaker" taking that little dewar from -180 to +100 could
have a shattering result. Would be nice to have a spare for that purpose.
Another point to be made: close of the high vacuum system when heating the cold
finger, why get the rubbish of the cold finger into your pump, especially if
its an ion getter type.
Cheers
Jim Darley
ProSciTech

On Thursday, July 13, 2000 7:48 PM, Chris Jeffree
[SMTP:cjeffree-at-srv0.bio.ed.ac.uk] wrote:
} True. So this could be an argument for using turbomolecular
} pumps, which are tolerant of water vapour. Is it advisable to subject
} the cold finger dewar to thermal shock by putting boiling water into
} it?
} Chris
}
} From: jim {jim-at-proscitech.com.au}
} Send reply to: "jim-at-proscitech.com.au" {jim-at-proscitech.com.au}
} To: "'Becky Holdford'" {r-holdford-at-ti.com} ,
} Microscopy ListServer
} {microscopy-at-sparc5.microscopy.com}
} Subject: RE: Cold traps and cold fingers in SEM
} Date sent: Thu, 13 Jul 2000 11:26:16 +1000
} Organization: ProSciTech
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Not a good idea to have the pumps loaded up with so much water. It does not
} }
} } take long to warm the cold finger: A beaker with boiling water on the
} } external
} } finger will rise the internals of the trap within 5 minutes to above room
} } temperature.
} } Cheers
} } Jim Darley
} } ProSciTech Microscopy PLUS
} } PO Box 111, Thuringowa QLD 4817 Australia
} } Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
} } Great microscopy catalogue, 500 Links, MSDS, User Notes
} } ABN: 99 724 136 560 www.proscitech.com
} }
} } On Thursday, July 13, 2000 4:50 AM, Becky Holdford [SMTP:r-holdford-at-ti.com]
} }
} } wrote:
} } }
} } }
} } } Randy and Listies:
} } } I too have an S4700 with a cold finger. But I don't have the time to let
} } } the
} } } cold finger come to room temperature if I need to retrieve a dropped or
} } } stuck
} } } sample. Plant A/C keeps the lab humidity below 60% and if I work fast,
} } } there
} } } is
} } } minimal frost buildup. The main chamber is back under working vacuum in
} } } less
} } } than 1 hour. The scope is equipped with a diffusion pump instead of a
} } } TMP.
} } } My
} } } question: Am I causing any problems by letting the pump system sublime
} } } the
} } } frost off the cold finger?
} } }
} } } "Tindall, Randy D." wrote:
} } }
} } }
} } } }
} } } } Chris,
} } } }
} } } } We have an Hitachi S4700 FESEM, and we routinely use the LN2
coldfinger.
} } } }
} } } } In
} } } } my experience, it reduces deposition of contamination under the beam,
} } } } especially during low voltage operation (1-5kV, say).
} } } }
} } } } The disadvantages so far have been vibration introduced by the boiling
} } } } LN2,
} } } } although this pretty much disappears after about 20-30 minutes, and the
} } } } problem of retrieving a sample which has become dislodged inside the
} } } } chamber. Although the latter occurrence is rare, it obviously requires
} } } } opening up the entire chamber to atmosphere, which isn't a great thing
} } } } to
} } } } do
} } } } with a chilly cold finger combined with the humidity of mid-Missouri.
} } } } In
} } } } this case, you need to wait until the system comes to room temperature,
} } } } effectively shutting down the scope for several hours.
} } } }
} } } } Randy
} } } }
} } } } Randy Tindall
} } } } EM Specialist
} } } } Electron Microscopy Core Facility
} } } } W122 Veterinary Medicine
} } } } University of Missouri
} } } } Columbia, MO 65211
} } } } Tel: (573) 882-8304
} } } } Fax: (573) 884-5414
} } } } Email: tindallr-at-missouri.edu
} } } } Web: http://www.biotech.missouri.edu/emc/
} } } }
} } }
} } } --
} } } ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
} } } Becky Holdford (r-holdford-at-ti.com)
} } } 972-598-1291 (pager)
} } } KFAB Physical Analysis Lab--SEM/FIB/FA
} } } Texas Instruments, Inc.
} } } Dallas, TX
} } } ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
} } }
} } }
} }
} }
}
}
} =====================================================================
} DR CHRIS JEFFREE
} BIOSEM - BIOLOGICAL SCIENCES EM FACILITY
} UNIVERSITY OF EDINBURGH
} Daniel Rutherford Building
} King's Buildings, Mayfield Road
} EDINBURGH, EH9 3JH, Scotland, UK
} Tel. #44 131 650 5345
} FAX. #44 131 650 6563
} Mobile 07710 585 401
} email c.jeffree-at-ed.ac.uk
} SEM / TEM bookings sem-at-ed.ac.uk
} =====================================================================




From daemon Thu Jul 13 08:00:25 2000



From: John Heckman :      heckman-at-pilot.msu.edu
Date: Thu, 13 Jul 2000 08:52:50 -0700
Subject: Re: EBSD ?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Jordi,

Electron BackScatter Diffraction. There's a recent overview and application
articles in Journal of Microscopy 195 (3), 170-185 (September, 1999) also
quite a bit of info at http://www.hkltechnology.com. These folks make hardware
and software for this.

cheers,
John

no commercial interest, just a student

"Marti, Jordi" wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Could somebody briefly educate me on EBSD (Electron Backscattered ...?) . I
} am trying to find information for a friend. He would like to know the very
} basics, capabilities of the technique (for example, resolution) and if there
} are contract labs that do this. Since he is interested in quantitative
} texture analysis, crystal orientation and GB analysis I assume that he is
} talking about getting and analyzing channeling patterns, but I could be
} wrong. I did not get from him what materials he is interested in. So, any
} information on EBSD would be appreciated.
}
} Thanks
}
} Jordi Marti
} Honeywell




From daemon Thu Jul 13 08:23:11 2000



From: Andreas Loewe :      loewe-at-uni-bonn.de
Date: Thu, 13 Jul 2000 08:11:40 -0500
Subject: What you really should do with L2N

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,

I will add my two cents to the L2N discussion:

Some years ago I stumbled over an article in the German issue of
"Scientific American". I think it was Peter Barham, who wrote about things
you can do with physics in the kitchen.
So we tried to make ice with LN2 and the result was just wonderful. And you
have a nice visual effect and your coworkers will praise you ...

Here is the howto for a small department:



We found strawberry ice the best
- take 2 kilos of strawberries, clean them and mash them
- take at least 1/2 kilo of sugar
- 2 liters of milk
- 1/2 liter of sweet cream
- add vanilla sugar and skin of a lemon

- precool it in the fridge
- fill all the ingredients into a metal bowl, plastic will crack, use gloves
- add the LN2, you may need about the same amount as the ingredients
- mix and mix and mix ...
- important: you have to stir like an idiot to get small ice crystals

- bon appetite




Andreas Loewe


Ps: You'll find more about this in a book by:
Herve This-Benckhard, "Les secrets de la casserole", Springer, 1993


===========================================================================
Andreas Loewe e-mail : loewe-at-uni-bonn.de
Department of Inorganic Materials Research Phone : (+49-228)-73-4180
University of Bonn Fax : (+49-228)-73-4205
Roemerstr. 164
53117 Bonn
===========================================================================





From daemon Thu Jul 13 08:23:28 2000



From: Margaret Miller :      miller-at-pathology.v20.uthscsa.edu
Date: Thu, 13 Jul 2000 08:12:14 -0500
Subject: Clinical performance standard

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does anyone have information regarding performance standards for
clinical technicians. ie. How many cases per month per technician?
Thanks for any feedback.





From daemon Thu Jul 13 12:19:28 2000



From: Wayne England :      wengland-at-ortech.on.ca
Date: Thu, 13 Jul 2000 13:00:41 -0400
Subject: SEM Position Opening - Toronto

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Bodycote Material Testing is a multidisciplinary contract research
organization serving a diverse range of industries and clients. Our
services include both routine testing as well as development/trouble
shooting.

We currently have a position for a scanning electron microscopist in our
Mississsauga location.

You will take a key role in the investigation of the physical properties of
both unknown and new material entities including. You will help clients to
problem solve and define their physical characterization needs, interpret
results, prepare quotations and write project reports.

Your practical expertise lies in scanning electron microscopy and energy
dispersive X-ray spectroscopy and includes experience in a materials
investigation. You enjoy problem solving and research activities, manage
your time well and are able to work effectively in a team atmosphere.
Additional experience with cryo-SEM, TEM, LM and associated characterization
techniques would be definite assets. A good business sense and working
knowledge of cGMP are also desirable.


Interested parties please submit your CV and salary requirements to Wayne
England.


__________________________________________

Wayne England
Manager, Physical Characterization
Bodycote Ortech
ph:(905)822-4111 X555 fax: (905)823-1446
http://www.na.bodycote-mt.com
mailto:england.w-at-bodycote-mt.com
______________________________________________




From daemon Thu Jul 13 14:05:38 2000



From: David Cugier :      david.cugier-at-abbott.com
Date: Thu, 13 Jul 2000 13:55:04 -0500
Subject: Many Thanks to the NIST traceable TEM mag std. responses

Contents Retrieved from Microscopy Listserver Archives
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I appreciate all the responses to the question I had regarding NIST traceable
TEM mag. stds.
Thank you so much,
David Cugier
Abbott Laboratories.



From daemon Thu Jul 13 14:49:32 2000



From: Tom Phillips :      PhillipsT-at-missouri.edu
Date: Thu, 13 Jul 2000 14:39:47 -0500
Subject: DiI or DiO labeling of membranes on cryosections??

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


does anyone know if probes such as DiI or DiO have ever been used to
label membranes on sectioned material such as cryosections?

TIA, Tom

Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)



From daemon Thu Jul 13 17:27:07 2000



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Thu, 13 Jul 2000 15:11:51 -0700
Subject: Re: EBSD ?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Jordi,
We have a EBSD system installed here. It is an SEM accessory that picks up
the Kikuchi lines that scatter off a flat crystalline surface. It is the
same as electron channeling, but picked up from the side by a TV camera. The
sample must be strain-free (eg. electropolished) and tilted 70 degrees
towards the fluorescent screen that shows the lines. A TV camera behind the
screen picks up the lines, enhances and analyses them as the beam is steered
across the sample, usually in a square raster at about 0.5 sec. per point.
This data can be analysed and displayed a number of ways, such as grain
maps, grain boundary maps, histograms of the various parameters, pole
figures and many other forms. The HKL web site (www.hkltechnology.com) has a
good overview and articles about the use of EBSP, also Oxford, who make a
system called Opal, have a promotional CD that covers the technique well.
You can request it from Oxford.
The resolution depends on the SEM, but you need a high beam current and
voltage to generate strong enough lines for the pickup system, so with the
high tilt the resolution is about one micron with my W filament SEM. Field
emmision SEMs will have higher resolution. The samples on my SEM have to be
quite small and thin ( {8 mm.), but we have successfully analysed IF steels,
various Al alloys and some minerals. These are well polished with colloidal
silica and lightly carbon coated.
Please contact me if you need any more information.
At 04:38 AM 7/13/00 -0700, you wrote:

} Could somebody briefly educate me on EBSD (Electron Backscattered ...?) . I
} am trying to find information for a friend. He would like to know the very
} basics, capabilities of the technique (for example, resolution) and if there
} are contract labs that do this. Since he is interested in quantitative
} texture analysis, crystal orientation and GB analysis I assume that he is
} talking about getting and analyzing channeling patterns, but I could be
} wrong. I did not get from him what materials he is interested in. So, any
} information on EBSD would be appreciated.
}
} Thanks
}
} Jordi Marti
} Honeywell
}
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca




From daemon Thu Jul 13 17:46:36 2000



From: Walck, Scott D. :      walck-at-ppg.com
Date: Thu, 13 Jul 2000 18:36:30 -0400
Subject: RE: EBSD ?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


EBSD is very much like the production of Kikuchi lines in the TEM. The
backscatter electrons while they are still inside the sample act as point
sources that are attached to the sample. A fraction of them can diffract
off the "front" and "back" side of crystal planes as they come out of the
crystal. You get cones of diffraction intensity where the Bragg condition
for a given crystallographic plane. The pattern is a fairly week signal on
the total backscattered intensity and it carries the crystallographic
symmetry and orientation of the illuminated volume. The sample is typically
tilted to a high angle ~70 degrees. A piece of film or camera is used to
capture the image. The cameras are better, because they can process the
image to bring out the details and the analysis automated to determine phase
or crystallographic orientation. There are mapping programs that can give
you orientational maps of the grain structure of your samples. The
resolution is essentially the same as that of a backscattered image.
Anything at the surface that distorts or disturbs the quality of the lattice
(contamination, oxidation, damage, etc.) will be reflected in the quality of
the pattern.

Incidentally, you should take a look at the M&M MM program. Joe Michael has
a special session on "Advances in the Instrumentation and Applications of
Electron Backscatter Diffraction in the SEM" that should be of interest to
you. There are also a number of vendors who have systems. You can see them
at the meeting.


-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)



} -----Original Message-----
} From: Marti, Jordi [mailto:jordi.marti-at-honeywell.com]
} Sent: Thursday, July 13, 2000 7:39 AM
} To: 'Microscopy'
} Subject: EBSD ?
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.ht} ml
}
}
} --------------------------------------------------------------
} ---------.
}
}
} Could somebody briefly educate me on EBSD (Electron
} Backscattered ...?) . I
} am trying to find information for a friend. He would like to
} know the very
} basics, capabilities of the technique (for example,
} resolution) and if there
} are contract labs that do this. Since he is interested in quantitative
} texture analysis, crystal orientation and GB analysis I
} assume that he is
} talking about getting and analyzing channeling patterns, but
} I could be
} wrong. I did not get from him what materials he is interested
} in. So, any
} information on EBSD would be appreciated.
}
} Thanks
}
} Jordi Marti
} Honeywell
}



From daemon Thu Jul 13 21:14:45 2000



From: Rick Powell at Nanoprobes :      rpowell-at-nanoprobes.com
Date: Thu, 13 Jul 2000 22:00:43 -0500
Subject: TEM: Thanks...Mercury diffusion pump conversion, Phillips 300

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello List Members:

Thanks for the suggestions on vendors and chillers - they were all very
helpful, and I will follow up individually. Now, we are faced with the
issue of whether we should convert the mercury diffusion pump in our
proposed Phillips 300 TEM (it stilll has the mercury pump). If anyone has
any opinions or experiences on this that they would like to share, I would
be very grateful.

Thanks again,

Rick Powell
Nanoprobes, Incorporated


**********************************************************************
* - PLEASE NOTE OUR NEW ADDRESS - EFFECTIVE FEBRUARY 15, 2000 - *
* *
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**********************************************************************





From daemon Fri Jul 14 03:00:31 2000



From: richard.beanland-at-gecm.com
Date: Fri, 14 Jul 2000 08:50:14 +0000 (GMT)
Subject: Surface Science Journals saved from the skip

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ron
Thanks for this - I clearly need some retraining on high vacuum
theory! Can you recommend a good review or textbook on this topic
that a biologist could understand?
Best wishes
Chris

} From: "Ronald Vane" {RVaneXEI-at-concentric.net}
To: {c.jeffree-at-ed.ac.uk}


Dear All,
after receiving my 6th email this afternoon about the surface science journals, I'd like to say they have already found a good home with Rik Brydson in Leeds. Glad to see there is such a thirst for knowledge out there...

Richard


} Dear all,
} I have a set of the Journal 'Surface Science' and 'Surface
} Science reports', 1990-1993 (inclusive). If anyone wants it, please
} get
} in touch with me by 20th of this month or it will go in the skip.
}
} Best regards,
}
} Richard Beanland

==============================================================
Richard Beanland
Caswell Technology,
Caswell,
Towcester,
Northants NN12 8EQ

e-mail richard.beanland-at-gecm.com
Tel. +44 1327 356363
Fax. +44 1327 356398
==============================================================
"The information contained in this message is legally privileged and confidential information intended for the eyes of the individual or entity named above. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message is strictly prohibited. If you have received this message in error, please notify us immediately by telephone.
Caswell Technology is the trading name of Marconi Caswell Limited. Registered in London No. 3694360 Registered Office: One Bruton Street London W1X 8AQ. Holding Company: Marconi plc."







From daemon Fri Jul 14 03:02:56 2000



From: Steve Chapman :      PROTRAIN-at-CompuServe.COM
Date: Fri, 14 Jul 2000 03:49:46 -0400
Subject: Cold Fingers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi

I have been watching with interest the talk about warming up a cold finger
and what comes to mind is WHY?

Having used cold traps and fingers on vacuum systems as well as a wide
variety of microscopes for many years, and never force warmed them, why
suddenly is this important?

A paper by Hitachi in the 60's related cold finger temperature to vacuum
level and contamination rate. The theory went that the vacuum level and
contamination rate would fall whilst you were using liquid nitrogen in the
traps, but once you stopped using LN2 it would take the same amount of time
for the vacuum level and contamination rate to fall back to the original
levels. So why heat the cold finger?

I also worked on the design of early SEM cryo systems where once again we
do not heat the cold finger; I keep asking why?

Is there a proven scientific reason that helps us out in this area,
practical reason I emphasize?

I do find it rather interesting!

Steve Chapman
Senior Consultant Protrain
For professional training and consultancy in EM world wide
www.emcourses.com
Tel +44 1280 814774 Fax +44 1280 914007



From daemon Fri Jul 14 04:04:05 2000



From: Ron Doole :      ron.doole-at-materials.oxford.ac.uk
Date: Fri, 14 Jul 2000 09:51:51 +0100 (BST)
Subject: 2010 HT and cold traps

Contents Retrieved from Microscopy Listserver Archives
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Hi All,

There have been several messages on these topics lately. The
original message was specifically a TEM cold trap question but it has
broadened out to SEM cold traps as well.

In answer to Yan's question;
JEOL 2010 ACD. We have a JEOL 2010 and as I have previously reported
we bake it out overnight. We always shut the HT down at the end of every
day and restart it the following one. We have always done this on every
instrument we have had. Over the past few years the instruments have
become cleaner and higher performance, we leave our 2010 with the lenses
set at the 200kV setting and at high (300K - 500K) mag. We do the same
with our 4000 HREM and 3000F (but we leave the HT on as it is a FEG). This
routine keeps the lenses warm so that they are stable when we want to use
them and do not outgas when they increase temperature with higher lens
currents.

Operation of cold traps.
I would never think of venting a column (or SEM chamber) when the cold
trap is cold. Although I am primarily interested in TEM the same
principles must apply to a SEM. If you need to vent the chamber to insert
a specimen then obviously this may affect your use of cold traps. Cold
surfaces trap gasses, they do this by lowering the vapour pressure. If you
have ice build up on a surface it will be more difficult to pump than a
clean surface, you will have a build up of gasses already on the surface
that will not only outgas for a long time but also decrease it's
efficiency as a cold trap.

It is recognised that to get a good clean vacuum we must bakeout the
vacuum system to increase the vapour pressure of the trapped
(absorbed) gasses in the surfaces and pump them away. When the surfaces
are then cooled down to room temperature the vapour pressure of any
trapped gasses is lower than the vacuum system pressure so the gasses are
firmly trapped. If we have gasses already trapped on a cold surface they
will be released into the vacuum system (albeit at a reducing rate) all
the time that system is pumping. This will give rise to contamination
during the operation of the instrument which can only be cured by raising
the temperture of the cold trap and cooling it down again.

Regards,
Ron


===========================================================================
Mr. Ron Doole e-mail ron.doole-at-materials.ox.ac.uk
Department of Materials, phone +44 (0) 1865 273701
University of Oxford, fax +44 (0) 1865 283333
Parks Road.
Oxford. OX1 3PH. UK.
============================================================================






From daemon Fri Jul 14 04:33:47 2000



From: Jonathan Barnard :      Jonathan.Barnard-at-angstrom.uu.se
Date: Fri, 14 Jul 2000 11:06:50 +0200
Subject: SEM:EBSD & Kossel

Contents Retrieved from Microscopy Listserver Archives
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Jordi,

There are two techniques associated with looking at the angular
distribution of emerging radiations from a crystal.

Firstly there is Kossel diffraction, which is demanding experimentally.
This requires a material with one dominant atomic element and what is
looked at here is the angular distribution of emerging X-rays generated
within the excitation volume. Kossel diffraction can be extremely accurate
for lattice parameter measurements (I have heard of up to five/six
significant figures in accuracy in certain cases).

Then there is EBSD in which inelastic back scattered electrons follow the
crystallographic planes a la Kikuchi in the TEM. A scintillator with a high
angular solid angle is used to capture the diffraction pattern. EBSD maps
requires a scan over an area with a high pixel dwell time, from minutes to
hours for a whole map. The technique requires a lot of memory since it
records a pattern for point over pre-determined area. The computer usually
fits a pre set range of materials of any crystallographic space group i.e.
it helps if you know what you have in the first place. The pattern
recognition works by an algorithm called a Hough transform, in which the
intensity at every point is turned into a pair of angular coordinates.
Lines of intensity show up a bright dots in Hough-space and the computer
fits the different crystal types to this pattern. Once the whole area is
done, it then gives you a scatter plot of all the crystal orientations. The
better systems will allow you to do statistical analysis like correlation
functions .etc.

A flat sample is quite a stringent condition and the seventy degree surface
normal to beam angle means you get BS SEM resolution only parallel to this
tilt axis i.e. side to side. Spatial resolution is limited in the other
direction to around 1 micron in real terms. If you are trying to map micro
precipitates less than a micron, then forget it. So the ideal sample has to
be flat and have grains in excess of one micron in size.

As for systems on the market, the ones I have seen are TEXSEM and the
Oxford Instruments systems. As far as I am aware, TEXSEM was the first
commercial company to do EBSD mapping in the beginning and the others have
come after them. TEXSEM was acquired by Edax a couple of years ago, but I
am sure Edax can give you information on the EBSD system and maybe their
new TEM crystallography mapping system as well. I know the TEXSEM system
through Prof David Dingley one the founders on TEXSEM, and also the one who
taught me most about SEM techniques when I was at Bristol. I don't know if
he is on this listserver, but he's the one person who can tell you anything
and everything about EBSD.

Regards,
Jonathan
********************************************************
Dr Jonathan Barnard

Analytical Materials Physics
The Angstrom Laboratory, Uppsala University
P O Box 534, SE-751 21 Uppsala, Sweden
Phone: +46-(0)18-4716838 Fax: +46-(0)18-500131
Phone: Microscope room +46 18 471 6365
http://www.angstrom.uu.se/analytical/home.html
********************************************************




From daemon Fri Jul 14 05:09:07 2000



From: Andrew Logan :      andrewlogan-at-paradise.net.nz
Date: Fri, 14 Jul 2000 21:51:49 -0700
Subject: LM : Russian Microscopes

Contents Retrieved from Microscopy Listserver Archives
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I am hoping to upgrade to a better microscope but have a limited budget. I
have seen Russian LOMO microscopes advertised on the web. Does anyone have
experience with these that they can share - in particular are their
objectives up to scratch?
Thanks
Andrew Logan






From daemon Fri Jul 14 06:31:05 2000



From: Chris Jeffree :      cjeffree-at-srv0.bio.ed.ac.uk
Date: Fri, 14 Jul 2000 12:12:09 +0000
Subject: Specimen and chamber contamination

Contents Retrieved from Microscopy Listserver Archives
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1) I presume that just as at atmospheric pressure, materials such
as perspex, anodised aluminium, borosilicate glass, stainless
steel, gold) differ significantly in their capacity to adsorb water
vapour and hydrocarbons under high vacuum conditions?
Presumably they would differ not only in their uptake kinetics but
also in their desorption kinetics. If one were designing an ultraclean
vacuum system, or an ultraclean specimen environment within a
specimen chamber, with the objective of minimising deposition to a
specimen surface during SEM examination, what materials would
be best to use for the chamber surfaces?
2) Presumably the main source of hydrocarbon contamination in an
SEM is oil vapour back-streaming from the the rotary and diffusion
pump. Turbo pumps, particularly maglev pumps, must reduce this
to very low levels while the pumps are running at full speed.
However, there must be considerable potential for back streaming
from the backing line during rough pumping from air and turbo run-
up after specimen changes. This again would seem to me to argue
in favour of a specimen air-locked system, so that the specimen
chamber is exposed to air and rough-pumping cycles as little as
practicable. Does anyone know of any data in the literature
comparing the magnitudes of contamination in systems using
different pumping and specimen exchange methods?
Chris
==========================================
Dr. Chris Jeffree
University of Edinburgh
BIOSEM - Biological Sciences Electron Microscope Facility
Institute of Cell and Molecular Biology
Daniel Rutherford Building
King's Buildings, Mayfield Road
EDINBURGH, EH9 3JH, Scotland, UK
Tel. #44 (0) 131 650 5345
FAX. #44 (0) 131 650 6563
Mobile 07710 585 401
email c.jeffree-at-ed.ac.uk
=========================================



From daemon Fri Jul 14 07:23:38 2000



From: Marti, Jordi :      jordi.marti-at-honeywell.com
Date: Fri, 14 Jul 2000 05:09:06 -0700
Subject: Thanks-EBSD

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I want to thank every one who responded with information on EBSD

Thanks

Jordi Marti
Honeywell



From daemon Fri Jul 14 08:56:04 2000



From: HOWARD L. MULHERN :      mulhern-at-hub.tch.harvard.edu
Date: Fri, 14 Jul 2000 09:44:38 -0400
Subject: Clinical Performance Standards

Contents Retrieved from Microscopy Listserver Archives
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Hello,

I'm not aware of a standard but at Childrens Hospital in Boston I
have processed from beginning to end about 350 EM clinical cases per
year for the past fifteen years. That is from embedding through
photography and printing with fifteen to thirty photographs per case.
The voluum of cases depends on several factors. The type of tissue,
the number of blocks per case, possible special techniques (ex. glycogen
stains), available equiptment ...tissue processors, knives, microtomes
etc. and of course the technologists ability. A number of labs that I
have visited require the technologist to limit their involvement to
embedding ,sectioning and printing the negatives. The EM lab from a
neighboring institution handles about 850 EM clinical cases, far
exceding what their technologist could handle and so they use their
residents to scope many of the cases. In my own situation I feel our
voluum is very high for one individual and the results are reflected in
my inability to get the cases out in a timely manner or take a vacation.
I have always felt that it was a waste of time for a resident/rotator
to come into the lab and scope cases without serious supervision. This
has limited our ability to handle more cases but common sense and
expierence has told me that someone who doesn't know the ultrastructural
pathology, the microscope and is not a technologist and who just comes
in the lab to take some photo's is using their time inneficiently.
After all, taking an electron micrograph is about communication. If the
technologist is capable and has the expierence to understand the
pathology, certainly not in making a diagnosis, but in presenting to the
pathologist a group of photo's that will stand out and either confirm or
alter a suspected diagnosis that certainly is communication and is an
efficient cost effective way of running the EM lab.
Performance standards? The very words sort of indicate to me that
all technologists and labs should be equal .
That would take the fun out.

Howard L.Mulhern
Childrens Hospital Dept. of Pathology
EM Facility
mulhern-at-hub.tch.harvard.edu





From daemon Fri Jul 14 09:07:00 2000



From: jim :      jim-at-proscitech.com.au
Date: Fri, 14 Jul 2000 23:57:54 +1000
Subject: RE: Cold Fingers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


'cause Hitachi were talking about the cold finger warming under operating
conditions. We were discussing opening the chamber when the finger is cold,
which would then load up with ice.
Also if warm, the roughing pump on immediate re-pumping would have a chance to
remove some contaminants from the "warm coldfinger"
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Friday, July 14, 2000 5:50 PM, Steve Chapman [SMTP:PROTRAIN-at-CompuServe.COM]
wrote:
}
}
}
} Hi
}
} I have been watching with interest the talk about warming up a cold finger
} and what comes to mind is WHY?
}
} Having used cold traps and fingers on vacuum systems as well as a wide
} variety of microscopes for many years, and never force warmed them, why
} suddenly is this important?
}
} A paper by Hitachi in the 60's related cold finger temperature to vacuum
} level and contamination rate. The theory went that the vacuum level and
} contamination rate would fall whilst you were using liquid nitrogen in the
} traps, but once you stopped using LN2 it would take the same amount of time
} for the vacuum level and contamination rate to fall back to the original
} levels. So why heat the cold finger?
}
} I also worked on the design of early SEM cryo systems where once again we
} do not heat the cold finger; I keep asking why?
}
} Is there a proven scientific reason that helps us out in this area,
} practical reason I emphasize?
}
} I do find it rather interesting!
}
} Steve Chapman
} Senior Consultant Protrain
} For professional training and consultancy in EM world wide
} www.emcourses.com
} Tel +44 1280 814774 Fax +44 1280 914007




From daemon Fri Jul 14 09:10:19 2000



From: Valdes, Erica R Dr. SBCCOM :      erica.valdes-at-sbccom.apgea.army.mil
Date: Fri, 14 Jul 2000 10:00:56 -0400
Subject: need fluorescent LM specimen prep protocol

Contents Retrieved from Microscopy Listserver Archives
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Can anyone on the list help here?
We need a protocol for preparation of specimens for a training exercise. We
will be doing an assay using Salmonella typhimurium positive control
(5x10EE9 cells/ml) and fluorescein-labeled affinity purified antibody to
Salmonella CSA-1 (0.5 mg/ml). We have protocols for rehydration/dilution of
the reagents and for preparing slides after doing the assay in
flasks/vials/plates, but what we would really prefer is something very
simple that can be done in-situ on the slide. My first question is if
anyone has or can provide a protocol for this and my second question is if
anyone can tell us what would be the best dilutions to use for this
exercise.

Thank-you in advance,

Erica Valdes
US Army Soldier Biological Chemical Command
Edgewood CB Center





From daemon Fri Jul 14 09:28:15 2000



From: Vitaly Feingold :      vitalylazar-at-worldnet.att.net
Date: Friday, July 14, 2000 12:22 AM
Subject: TEM: Thanks...Mercury diffusion pump conversion, Phillips 300

Contents Retrieved from Microscopy Listserver Archives
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Rick,

We offer the Philips EM300 vac. system upgrade, which includes conversion to
Hg-less pumping system. Philips Electron Optics (now part of FEI company)
was offering the same upgrades, they probably still do.

Benefits one may expect from the upgrade:

1. Vacuum system performance (reliability, pumping speed, etc.) - none.

2. Health hazard reduction for the lab personnel - almost none. The Mercury
is not in contact with the outside world, except for a few exotic accident
scenarios or when grossly mishandled.

3. Possible future repairs of the vacuum system - upgrade makes for a great
advantage, since one will not have to comply with numerous safety rules in
order to work with the system containing toxic substance.

4. Speaking of exotic accidents - the probability of such is low, yet is
greater than zero nonetheless. Room cleaning is costly and troublesome,
shall room become Hg contaminated. An upgrade makes for a great advantage
from this standpoint.

Vitaly Feingold
Scientific Instruments and Applications
2773 Heath Lane, Duluth GA 30096
(770)232-7785 ph.
(770)232-1791 fax
-----Original Message-----
} From: Rick Powell at Nanoprobes {rpowell-at-nanoprobes.com}
To: Microscopy-at-sparc5.microscopy.com {Microscopy-at-sparc5.microscopy.com}






From daemon Fri Jul 14 09:35:47 2000



From: Bruce Brinson :      brinson-at-cnst.rice.edu
Date: Fri, 14 Jul 2000 09:29:56 -0500
Subject: Re: Cold Fingers

Contents Retrieved from Microscopy Listserver Archives
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Hello Steve,
This all started around the 2010. With our 2010, the ion pump cannot handle
the gas load while the ADC warms up on it's own. There is a regeneration cycle
that switches over to the DP but should a user fail to start that cycle.... it
goes like this... oh the current in the ion pump is too high, goto PD... the DP
can out pump the virtual leak (gas load from the ACD regeneration) & the
pressure drops. The system crosses over to the ion pump which can't handle the
gas load & the cycle repeats at ~0.5 Hz. Along with the switching of pumps the
gun isolation valve cycles. It has a bellows seal. Typical cycle life times of
a bellows is 10,000. They fail.
It is my opinion that this is a silly short coming of the system & could be
solved with a simple counting circuit... maybe a ring counter, one shot & an
AND GATE. Obviously a little programming could deal with this too. My
suggestions to the vender about this issue have not gotten a fix.... so every
couple of years we go down for a few days & they buy us a new valve... go
figure. BTW I think they now use an o-ring seal in a new valve design. I was
told it will not screw into the older machines.
For systems that are only pumped by a diffusion or turbo pump, I think this
is a non-issue & you can just walk away at the end of the day. This is true of
our FESEM.

Bruce Brinson
Rice U.

Steve Chapman wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hi
}
} I have been watching with interest the talk about warming up a cold finger
} and what comes to mind is WHY?
}
} Having used cold traps and fingers on vacuum systems as well as a wide
} variety of microscopes for many years, and never force warmed them, why
} suddenly is this important?
}
} A paper by Hitachi in the 60's related cold finger temperature to vacuum
} level and contamination rate. The theory went that the vacuum level and
} contamination rate would fall whilst you were using liquid nitrogen in the
} traps, but once you stopped using LN2 it would take the same amount of time
} for the vacuum level and contamination rate to fall back to the original
} levels. So why heat the cold finger?
}
} I also worked on the design of early SEM cryo systems where once again we
} do not heat the cold finger; I keep asking why?
}
} Is there a proven scientific reason that helps us out in this area,
} practical reason I emphasize?
}
} I do find it rather interesting!
}
} Steve Chapman
} Senior Consultant Protrain
} For professional training and consultancy in EM world wide
} www.emcourses.com
} Tel +44 1280 814774 Fax +44 1280 914007




From daemon Fri Jul 14 09:50:23 2000



From: beth-at-dogwood.botany.uga.edu (Beth Richardson)
Date: Fri, 14 Jul 2000 10:46:29 -0500
Subject: Philly roommate? (fwd)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Forwarded message:

} Hello, I'm a grad student attending the M&M2K meeting in Philadelphia next
} } month, and I'm looking for a roommate. I'm a non-smoking, non-snoring female
} } arriving Aug. 12. PLEASE REPLY TO gcelio-at-arches.uga.edu .
}
} Thanks,
} --Gail Celio

**************************************
Beth Richardson
EM Lab Coordinator
Botany Department
University of Georgia
Athens, GA 30602

Phone - (706) 542-1790
FAX - (706) 542-1805
Email - beth-at-dogwood.botany.uga.edu
**************************************





From daemon Fri Jul 14 11:00:28 2000



From: Bruce Brinson :      brinson-at-cnst.rice.edu
Date: Fri, 14 Jul 2000 10:53:24 -0500
Subject: SCSIorIDE

Contents Retrieved from Microscopy Listserver Archives
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Could I get opinions based on experience?
I have a nice scanner with a SCSI interface. The vender says my 20
MHz SCSI card exceeds the scanner through put. The scanner would be the
bottle neck on the SCSI buss no mater how fast the SCSI bus or drive is.
So my question : once you have the image on your hard drive, is in
terms of disk I/O for processing, by the time you go from the SCSI bus
to the mother board & back, is there a still a speed advantage to SCSI
over current IDE technology. Or put another way, can I justify buying a
super duty SCSI HD & interface in the name of processing speed & what
would be the relative improvement?


many thanks,
Bruce Brinson
Rice U.





From daemon Fri Jul 14 11:12:01 2000



From: Emma Lou Cardell :      cardelel-at-email.uc.edu
Date: Fri, 14 Jul 2000 12:03:36 -0400
Subject: LM Of Mouse Inner Ear

Contents Retrieved from Microscopy Listserver Archives
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Dear All,

During my LM analysis of serial, toluidine blue-stained plastic sections of
decalcified in tact mouse inner ears, I have encountered what appear to be
dark-staining, elongated "crystals" in the supranuclear region of some
Deiter's (support) cells in the organ of Corti. This region of the cells
typically has numerous round lysosomes that stain dark blue; the vast
majority of support cells have the usual round lysosomes present. Deiter's
cells with the long crystals show crystals also in serial sections of the
same cell.
The appearance of the spikey "crystals" in serial sections of the same
cell, the specificity of the inclusions for the support cells only, and the
limited supranuclear location within these highly polarized cells suggest
that these inclusions are not artifacts of staining. I have observed them
in wildtype and mutant animals, but not in every ear of any genotype. I
have done relatively little EM analysis of the ears so far, and no samples
with these inclusions have been checked yet by EM.
Are there lysosomal abnormalities that may result in their contents
becoming crystalline? Why do relatively few Deiter's cells have the
crystals? Apparently the genetic background of the mouse gene knock-out
models I am analyzing includes the development of hearing deficits with
aging. Is it possible that these "deviant Deiter's cells" may be related to
impending changes in the inner ear due to aging (the oldest animals I've
evaluated are 21 weeks old)? Have such inclusions been reported in Deiter's
cells (or other cell types)? I hope someone has an answer!
Thanks for your help or suggestions,

Emma Lou Cardell, Research Associate
Department of Cell Biology, Neurobiology, and Anatomy
University of Cincinnati Medical College
231 Albert Sabin Way
Cincinnati, OH 45267-0667





From daemon Fri Jul 14 11:26:30 2000



From: Ian Cotton :      icotton-at-gatan.com
Date: Fri, 14 Jul 2000 09:41:17 -0700
Subject: Job opening at Gatan

Contents Retrieved from Microscopy Listserver Archives
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Chris:

This is my favorite book:

Vacuum Methods in Electron Microscopy
Wilbur C. Bigelow
Portland Press, London, 1994

Ron Vane

-----Original Message-----
} From: Chris Jeffree {cjeffree-at-srv0.bio.ed.ac.uk}
To: Ronald Vane {RVaneXEI-at-concentric.net}
Cc: microscopy-at-sparc5.microscopy.com {microscopy-at-sparc5.microscopy.com}


An exciting and challenging opportunity is available with Gatan.

Product Manager,

Analytical Instruments

Gatan Inc. has an immediate opening for a person to handle GIF and PEELS
Product management. Individual should have either GIF or PEELS experience, a
strong TEM background in Biological or Materials science, good computer
skills, a willingness to travel and the vision and drive to help determine
the future of these products. The position is based in Pleasanton, CA and
carries a salary comensurate with experience as well as a bonus plan and
Corporate success share plan. Please contact Ian Cotton, Director of
Marketing at 925-224-7343 or email your resume to icotton-at-gatan.com.

*********************************
Ian Cotton
Marketing Director,
Gatan, Inc. 5933 Coronado Lane
Pleasanton, CA 94588 USA
Phone 925-224-7343
Fax 925-463-0204
E-mail icotton-at-gatan.com
Surf the Web to www.gatan.com
*********************************




From daemon Fri Jul 14 12:00:26 2000



From: diane.a.ciaburri-at-gdds.com
Date: 7/3/00 10:31 AM
Subject: Re: resin shelf life EPOFIX

Contents Retrieved from Microscopy Listserver Archives
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Everett,

We've had the same experience but no answers. We have a bottle right now that
is about one fourth filled with 'crystals'. Once it gets this full we dispose
of it and move on to a fresh bottle. I always wonder about the quality of our
mounts once the crystals start forming but it seems to work OK.

We store our bottles at room temperature and in the solvents cabinet.

Anybody else have any ideas?


Diane Ciaburri
General Dynamics
Pittsfield MA 01210
diane.a.ciaburri-at-gdds.com
(413)494-2847

____________________Reply Separator____________________

Hi,
I use Epofix epoxy. In a very short time after purchase the resin becomes cloudy
and a white sediment forms at the bottom of the bottle. I have heard this
sediment described as crystals. I have continued to use the resin---carefully
avoiding agitation and entrainment of the sediment when I withdraw it from the
bottle. Does anyone else have experience with this?

Everett Ramer
National Energy Technology Laboratory
P.O. Box 10940, Cochrans Mill Road
Pittsburgh, PA, USA 15236-0940
Voice: 412-386-4920
FAX: 412-386-4806
ramer-at-netl.doe.gov




From daemon Fri Jul 14 12:27:03 2000



From: diane.a.ciaburri-at-gdds.com
Date: Fri, 14 Jul 2000 13:13:02 -0400
Subject: Thanks - Deencapsulating Plastic IC's

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Thank you to all who sent ideas and procedures for de-encapsulating plastic
encapsulated IC's. I haven't received any samples yet so haven't been able to
try out any ideas. --The old hurry up and wait routine.

I'm always astounded by how helpful and knowledgeable everyone is. Thank you!
and thanks for managing this listserver, Nestor!


Diane Ciaburri
General Dynamics
Pittsfield MA 01210
diane.a.ciaburri-at-gdds.com
(413)494-2847




From daemon Fri Jul 14 12:57:43 2000



From: rlvaughn-at-unmc.edu
Date: Fri, 14 Jul 2000 12:48:06 -0500
Subject: Denton vacuum info

Contents Retrieved from Microscopy Listserver Archives
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Does any one know the current phone number and or web site of Denton
Vacuum?
The ones I am using : 888-336-8661, www.dentonvacuum.com are not
responding.
I need some advice on one of their old evaporators and possibly puchase a
couple of thermocouples (vacuum gage). Why do they call it a thermocouple?
The TEM service person that was here didn't think that was a proper name.
Thanks.

Rick Vaughn
rlvaughn-at-unmc.edu




From daemon Fri Jul 14 13:15:20 2000



From: White, Woody N :      Woody.N.White-at-mcdermott.com
Date: Fri, 14 Jul 2000 13:47:00 -0500
Subject: Re:Specimen and chamber contamination

Contents Retrieved from Microscopy Listserver Archives
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Chris,

A comment on your question...

Some turbo pumped vacuum systems stop the turbo and rough pump
through it when evacuating.
I feel you are correct in your comments about such systems.

Other designs implement a valved vacuum bypass so that the chamber is
directly roughed to about 100 microns. When, at this point, the flow is
more
random, the valves switch the turbo in series between the chamber and the
roughing pump and continue to high vacuum. The turbo is allowed to run
continuously, minimizing backstreaming.

The only disadvantage I know to this scheme is the added expense of
controling
circuitry and
the (major) expense of a large hi-vac gate valve.

My old ETEC was plumbed like this and I replaced the dif pump with a Leybold
mag-lev
turbo (too much vibration from the ball bearing pumps). "Cooked" very
little
carbon on to the
specimen surfaces and the vacuum (excluding outgassing specimens) was
typically
1.0 E-6
Torr or better.

Woody White
McDermott Technology, Inc.



From daemon Fri Jul 14 13:17:21 2000



From: Monette, Robert :      Robert.Monette-at-nrc.ca
Date: Fri, 14 Jul 2000 14:07:42 -0400
Subject: unsubscribe

Contents Retrieved from Microscopy Listserver Archives
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From daemon Fri Jul 14 13:36:50 2000



From: Leona Cohen-Gould :      lcgould-at-mail.med.cornell.edu
Date: Fri, 14 Jul 2000 13:33:21 -0400
Subject: real-time confocal

Contents Retrieved from Microscopy Listserver Archives
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Hi All,
I have a colleague who needs to do real-time confocal or multi-photon. Her
minimum wavelength requirement is 488, although additional wavelengths
would be useful. We are located in New York City, but she is open to
anywhere in the northeast.
If anyone can help her, please contact her directly:

Dr. Geri Kreitzer
Dyson Vision Institute
Weill Medical College of cornell University
1300 York Ave
New York, NY 10021

(212)746-2277

e-Mail: ggurlan-at-med.cornell.edu


Thanks in advance,
Lee

Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175





From daemon Fri Jul 14 13:41:43 2000



From: Greg Erdos :      gwe-at-biotech.ufl.edu
Date: Fri, 14 Jul 2000 14:37:18 -0400
Subject: denton

Contents Retrieved from Microscopy Listserver Archives
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I used Denton's site yesterday and today. No problem.
Gregory W. Erdos, Ph.D.
Assistant Director, Biotechnology Program
PO Box 110580
University of Florida
Gainesville, FL 32611

Ph. 352-392-1295 Fax: 352-846-0251



From daemon Fri Jul 14 14:48:33 2000



From: Gang Ning :      gning-at-mcw.edu
Date: Fri, 14 Jul 2000 14:41:06 -0500
Subject: LR White cell culture

Contents Retrieved from Microscopy Listserver Archives
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Hi - I am looking for a good/successful protocol (from fixation to
polymerization) for LR white immunocytochemistry for cell culture. Can
anyone help?. Thanks very much in advance.

G. Ning
EM Facility
Medical College of Wisconsin
414-456-8344
Fax 414-456-6535




From daemon Fri Jul 14 15:11:32 2000



From: Nazlia Samodien :      ctssamodien-at-samiot.uct.ac.za
Date: Fri, 14 Jul 2000 14:54:30 -0500
Subject: Jeol JSM5200

Contents Retrieved from Microscopy Listserver Archives
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Hi All
We desperately need some help with our SEM.
Our filament "appeared" to have blown during operation (I was out of the
room for 5 mins and came back to a pretty green screen). I initially
assumed that the electronics were playing up, which sometimes occurs, and
all the settings are thrown out. While tweaking the Contrast/brightness
controls, some kind of warning flashed on the screen. However, it
dissappeared too quickly for me to figure it out, and it has not
reappeared. We changed our filament after no emission current reading was
found. No luck. The technicians then advised us to change and recheck the
filament settings in the cap. Alas, still nothing. We have, however, found
that the LC reading shoots up to about 190ua, during the 4th filament check
but still no image on the screen. Between changing , cleaning & checking
filaments, there has been an eerie inconsistency with the LC. Between three
of us, we have twiddled here and twiddled there without success. Help!!!!!
Any info would be greatly appreciated.
Nazlia


_____________________________________________________

Nazlia Samodien

Cardiovascular Research Unit
Dept. of Cardiothoracic Surgery
University of Cape Town Medical School
Anzio Rd, Observatory, 7925, South Africa

Tel. +27 21 406 6398 (office)
+27 21 406 6476 (Secretary)
Fax + 27 21 448 5935


Email: ctssamodien-at-samiot.uct.ac.za





From daemon Fri Jul 14 15:34:03 2000



From: =shAf= :      mshaf-at-darkwing.uoregon.edu
Date: Fri, 14 Jul 2000 13:57:55 -0700
Subject: RE: SCSIorIDE

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Bruce,

The newer IDE technologies(Ultra DMA 66) approaches the "burst speed"
read/write capabilities of the newer SCSI technologies. However, the newest
emerging Ultra DMA 100 technology provides data transfer rates beyond SCSI.
These new drives are going to hit the market any day now. Of course, you
will need to buy a DMA 100 controller card to go along with the DMA 100 hard
drive, unless you opt to buy an new computer with the capabilities built
into the motherboard. If memory serves me right, Quantum Corp. patented the
technology, but I think Maxtor is going to be first to the market with the
drives. Look to Promise Technologies(www.promise.com) for the appropiate
controller card. Good luck.


Gary M. Easton
Scanners Corporation
Third Party SEM Service
410-857-7633 x102
----- Original Message -----
} From: "Bruce Brinson" {brinson-at-cnst.rice.edu}
To: "MSA Listserver" {microscopy-at-sparc5.microscopy.com}
Sent: Friday, July 14, 2000 11:53 AM


Bruce writes ...

} I have a nice scanner with a SCSI interface. The vender
} says my 20 MHz SCSI card exceeds the scanner throughput.
} The scanner would be the bottle neck on the SCSI buss
} no matter how fast the SCSI bus or drive is.
} ...

I believe your "vendor" sells bridges too :o)

A slower SCSI device will not bottleneck the SCSI bus. The exception
to this is putting a 68pin non-ultrawide (i.e. non-U2W) device on a
U2W bus when you have U2W devices on it. This will slow "U2W" down to
"UW". However, note I am talking about the wide 68pin bus (40Mbs),
not the 50pin bus common to scanners. Even as slow as scanners are,
the modern ones are fast-SCSI compatible ... meaning, even if they're
not fast, they know how to communicate with a fast-SCSI controller and
not slow it down.
Of course ... this is the way it is supposed to work. If your
scanner's fireware is not communicating with the controller properly,
then who knows ... BUT generally speaking, your vendor is incorrect.

shAf :o)




From daemon Fri Jul 14 17:22:52 2000



From: Walck, Scott D. :      walck-at-ppg.com
Date: Fri, 14 Jul 2000 18:10:13 -0400
Subject: RE: Specimen and chamber contamination

Contents Retrieved from Microscopy Listserver Archives
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1. The numbers that you want are readily available in a number of Vacuum
science books. Probably the best one with the most data about different
materials is the one by Roth. Sticking coefficients, desorption,
adsorption, and chemisorption of the different gases depend on the gas,
temperature, material, and the surface roughness. That is why the relative
partial pressures of different gases are so different at different vacuum
regimes and vacuum histories (e.g. baked, not baked). If you want a great
material to coat the inside of your vacuum system with that has very good
properties, try gold. I once had a UHV system that was gold plated on the
inside. It was also very pretty.

2. You will only get hydrocarbon (HC) contamination from the mechanical
pump if you pump the system into the molecular range with this pump. The
pump speed for HCs is extremely high. If you pump the system through the
turbo pump, while the T-pump is starting, the mechanical pump is still in
the viscous range. It doesn't take the T-pump to get up to enough speed
before the pump speed will be sufficient to prevent the backstreaming of the
mechanical pump oil. When a T-pump system vents, what is important is that
there is a valve between the mechanical pump and T-pump to prevent oil from
being sucked out of the pump and back into the chamber. A properly designed
T-pump system should also have a gas inlet to help prevent this in case of a
valve failure when a power failure occurs. On T-pumped systems, this is
what you need to examine to see whether the system can be contaminated with
pump oil. Essentially, turn off the power (hypothetically, of course) and
see what happens. If it does not have several systems in place which
includes valves and automatic venting in the correct places, don't buy the
system.

With respect to pump down times, the pressure follows exponential curves.
These curves have dominant regimes. Initially, the volume gas is pumped,
but that is the very fast evacuation stage. Then it transitions over to
virtual leaks (i.e. trapped gas), then desorption off walls, then outgassing
of materials, then rate of gas diffusion through materials (permeation). If
you plot the log of the pressure with time, you will see these changeover
points. The longer the system is under vacuum, the better it gets, assuming
that there are no leaks that set up a steady state condition. Your ultimate
pressure is determined by a simple equation, SP=Q. S is the pump speed
(liters/sec), P(Torr) is the pressure, and Q(Torr-liters/sec) is the
throughput. The throughput is the sum of all leaks and outgassing at your
ultimate pressure. These are all temperature dependent and will increase at
higher temperatures -that's why you bake out.

If you pre-pump with an exchange system that is not designed to minimize
backstreaming, it can lead to contamination of the system when you finally
open the chamber valve.

If you have a chamber that pumps to the 10^-6 Torr region, and you can
operate in the low 10^-5 Torr range while it gets into the 10^-6 range with
time, then the only thing that the exchange system really buys you is time.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)



} -----Original Message-----
} From: Chris Jeffree [mailto:cjeffree-at-srv0.bio.ed.ac.uk]
} Sent: Friday, July 14, 2000 8:12 AM
} To: microscopy-at-sparc5.microscopy.com
} Subject: Specimen and chamber contamination
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} ---------.
}
}
} 1) I presume that just as at atmospheric pressure, materials such
} as perspex, anodised aluminium, borosilicate glass, stainless
} steel, gold) differ significantly in their capacity to adsorb water
} vapour and hydrocarbons under high vacuum conditions?
} Presumably they would differ not only in their uptake kinetics but
} also in their desorption kinetics. If one were designing an
} ultraclean
} vacuum system, or an ultraclean specimen environment within a
} specimen chamber, with the objective of minimising deposition to a
} specimen surface during SEM examination, what materials would
} be best to use for the chamber surfaces?
} 2) Presumably the main source of hydrocarbon contamination in an
} SEM is oil vapour back-streaming from the the rotary and diffusion
} pump. Turbo pumps, particularly maglev pumps, must reduce this
} to very low levels while the pumps are running at full speed.
} However, there must be considerable potential for back streaming
} from the backing line during rough pumping from air and turbo run-
} up after specimen changes. This again would seem to me to argue
} in favour of a specimen air-locked system, so that the specimen
} chamber is exposed to air and rough-pumping cycles as little as
} practicable. Does anyone know of any data in the literature
} comparing the magnitudes of contamination in systems using
} different pumping and specimen exchange methods?
} Chris
} ==========================================
} Dr. Chris Jeffree
} University of Edinburgh
} BIOSEM - Biological Sciences Electron Microscope Facility
} Institute of Cell and Molecular Biology
} Daniel Rutherford Building
} King's Buildings, Mayfield Road
} EDINBURGH, EH9 3JH, Scotland, UK
} Tel. #44 (0) 131 650 5345
} FAX. #44 (0) 131 650 6563
} Mobile 07710 585 401
} email c.jeffree-at-ed.ac.uk
} =========================================
}



From daemon Fri Jul 14 22:23:35 2000



From: Sang-Ha Leigh :      sleigh-at-ms.cc.sunysb.edu
Date: Fri, 14 Jul 2000 23:06:48 -0400
Subject: re: confocal microscope, any recommendations?

Contents Retrieved from Microscopy Listserver Archives
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I am currently looking into a confocal microscope system. Based on my
Internet search, I have a feeling that most of the confocal microscope
systems are primarily designed for "biology". My application will be
investigation of (i)microstructure of ceramic and metallic coatings (mostly
porous), and (ii) surface (including fracture surface) topology. I do not
have any experience in confocal microscopy. I would like to get some of your
opinions about confocal microscope systems for materials science
applications.

Thanks.
============================================
Sang-Ha Leigh, Ph.D.

Department of Materials Science and Engineering
State University of New York at Stony Brook
Stony Brook, NY 11794-2275

Tel: 1-631-632-8486
Fax: 1-631-632-8052
email: sleigh-at-ms.cc.sunysb.edu
============================================




From daemon Sat Jul 15 08:03:14 2000



From: Lalita Ramakrishnan :      lalitar-at-cmgm.stanford.edu
Date: Sat, 15 Jul 2000 07:40:59 -0500
Subject: EMs of frog tissue

Contents Retrieved from Microscopy Listserver Archives
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Dear all,

I am working on Mycobacterium marinum infection of frogs (Rana pipiens)
which gives them a chronic tuberculosis-like disease. The frogs develop
granulomas. We have done EMs of these frog granulomas and find certain
cell types that we cannot identify (macrophages vs heterophils?). If any
of you have expertise with amphibian tissues, could you get in touch with
me? We could send you pictures or come down and see you if you are close
by. We would greatly appreciate any help we can get. Thanks in advance.

Lalita Ramakrishnan





From daemon Sat Jul 15 10:11:32 2000



From: Earl Weltmer :      earlw-at-pacbell.net
Date: Sat, 15 Jul 2000 07:52:48 -0700
Subject: Re: Jeol JSM5200

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

You probably have a high voltage problem as indicated by the high LC current.
Possibly a capacitor in the oil tank or defective high voltage cable.

The repairs are not for a novice or user as the voltages are potentially
lethal. Call a service engineer.

Regards,

Earl Weltmer

Nazlia Samodien wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hi All
} We desperately need some help with our SEM.
} Our filament "appeared" to have blown during operation (I was out of the
} room for 5 mins and came back to a pretty green screen). I initially
} assumed that the electronics were playing up, which sometimes occurs, and
} all the settings are thrown out. While tweaking the Contrast/brightness
} controls, some kind of warning flashed on the screen. However, it
} dissappeared too quickly for me to figure it out, and it has not
} reappeared. We changed our filament after no emission current reading was
} found. No luck. The technicians then advised us to change and recheck the
} filament settings in the cap. Alas, still nothing. We have, however, found
} that the LC reading shoots up to about 190ua, during the 4th filament check
} but still no image on the screen. Between changing , cleaning & checking
} filaments, there has been an eerie inconsistency with the LC. Between three
} of us, we have twiddled here and twiddled there without success. Help!!!!!
} Any info would be greatly appreciated.
} Nazlia
}
} _____________________________________________________
}
} Nazlia Samodien
}
} Cardiovascular Research Unit
} Dept. of Cardiothoracic Surgery
} University of Cape Town Medical School
} Anzio Rd, Observatory, 7925, South Africa
}
} Tel. +27 21 406 6398 (office)
} +27 21 406 6476 (Secretary)
} Fax + 27 21 448 5935
}
} Email: ctssamodien-at-samiot.uct.ac.za




From daemon Sun Jul 16 13:35:26 2000



From: jyr15102-at-glaxowellcome.com ()
Date: Sun, 16 Jul 2000 13:12:28 -0500
Subject: Summer Student Needs Help....

Contents Retrieved from Microscopy Listserver Archives
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Email: jyr15102-at-glaxowellcome.com
Name: Jason Remillard
School: University of Alberta

Question: Good Day;

My name is Jason Remillard, and I am a pharmacy student from the
University of Alberta in Edmonton, Alberta, Canada. I am working as a
summer student at GlaxoWellcome in Toronto, Ontario and one of my projects
requires the use of the light microscope. Since I am not well trained in
this area, I have a couple of questions that you may be able to help me
with.

The purpose of my project is to help develop a faster method of
determining particulate count for Flonase(TM) Nasal Spray (fluticisone
propionate) - a corticosteroid nasal spray suspension for allergic rhinitis
and sinusitis. The current counting method is labour intensive, and time
consuming. It involves using a regular light microscope, and counting the
particles in the field (and there are a lot). Based on this data, the
particulate
count for the entire bottle is determined. This is currently
being done by the Quality Control Labs, who want to speed it up.

I have the following questions to ask:

Have you ever done any work with fluticisone propionate before?
I thought that by the use of oil immersion microscopy, I would be
able to get better magnification of the area, and have to count
less particles. I would still be able to calculate particle count,
but how would I go about using oil to increase the refractive index?

---------------------------------------------------------------------------





From daemon Mon Jul 17 04:04:03 2000



From: Labar Janos :      labar-at-mfa.kfki.hu
Date: Mon, 17 Jul 2000 11:20:01 +0200
Subject: Computer program to process SAED ring patterns

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Colleagues,

I have developed a computer program to process electron diffraction ring
patterns. The program can be downloaded FREE from

http://www.mfa.kfki.hu/~labar/

I hope you will enjoy it. Future versions with enhanced functionality are
coming. In case of any problem or suggestion or remark, do not hesitate to
contact me.

Best regards:

Dr. Janos L. Labar
Research Institute for Technical Physics and Materials Science
H-1121 Budapest, Konkoly-Thege u. 29-33
Postal address: H-1525 Budapest-114, Po Box 49
Tel: (36)(1) 392-26-92
Fax: (36)(1) 275-49-96
Fax/phone: (36)(1) 395-92-32
home page: www.mfa.kfki.hu/~labar





From daemon Mon Jul 17 08:24:03 2000



From: Robert Champaign :      r-champaign-at-raytheon.com
Date: Mon, 17 Jul 2000 08:07:48 -0700
Subject: Re: Denton vacuum info

Contents Retrieved from Microscopy Listserver Archives
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}
} The phone number I have for Denton is 856-439-9100. I recently used the
number so it should be good.
}
}
}
} At 12:48 PM 07/14/2000 -0500, you wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Mon Jul 17 08:50:15 2000



From: jyr15102-at-glaxowellcome.com () (by way of Brian McIntyre :      mcintyre-at-optics.rochester.edu )
Date: Mon, 17 Jul 2000 09:35:19 -0400
Subject: Summer Student Needs Help....

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hi-

wouldn't this be a good candidate for some type of optical particle
counting method (hiac type)?? or maybe a coulter type method?

just my 2c...

b-
******************************************

Email: jyr15102-at-glaxowellcome.com
Name: Jason Remillard
School: University of Alberta

Question: Good Day;

My name is Jason Remillard, and I am a pharmacy student from the
University of Alberta in Edmonton, Alberta, Canada. I am working as a
summer student at GlaxoWellcome in Toronto, Ontario and one of my projects
requires the use of the light microscope. Since I am not well trained in
this area, I have a couple of questions that you may be able to help me
with.

The purpose of my project is to help develop a faster method of
determining particulate count for Flonase(TM) Nasal Spray (fluticisone
propionate) - a corticosteroid nasal spray suspension for allergic rhinitis
and sinusitis. The current counting method is labour intensive, and time
consuming. It involves using a regular light microscope, and counting the
particles in the field (and there are a lot). Based on this data, the
particulate
count for the entire bottle is determined. This is currently
being done by the Quality Control Labs, who want to speed it up.

I have the following questions to ask:

Have you ever done any work with fluticisone propionate before?
I thought that by the use of oil immersion microscopy, I would be
able to get better magnification of the area, and have to count
less particles. I would still be able to calculate particle count,
but how would I go about using oil to increase the refractive index?

---------------------------------------------------------------------------








From daemon Mon Jul 17 08:54:39 2000



From: Leona Cohen-Gould :      lcgould-at-mail.med.cornell.edu
Date: Mon, 17 Jul 2000 08:50:31 -0400
Subject: Re: Denton vacuum info

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 12:48 PM -0500 7/14/0, "rlvaughn-at-unmc.edu"-at-sparc5.microscopy.com wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Rick,
I have some recent literature from Denton, and the telephone # listed is:
(856)439-9100
The web site you have looks OK. I have and e-mail for
them:info-at-dentonvacuum.com
YOu can also get in touch with Dick Daniel, he is the sale/sevice fellow
here in the NY Metro area. His number is" (201)847-8845. He is very
helpful.

I have nofinfncial interests in Denton Vacuum.

Lee

Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175





From daemon Mon Jul 17 09:11:12 2000



From: Rafal Dunin-Borkowski :      rafal.db-at-materials.oxford.ac.uk
Date: Mon, 17 Jul 2000 14:59:56 +0100 (BST)
Subject: 3rd Euroconference on NANOSCIENCE FOR NANOTECHNOLOGY

Contents Retrieved from Microscopy Listserver Archives
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3rd Euroconference on NANOSCIENCE FOR NANOTECHNOLOGY

16 - 19 September 2000
Somerville College, Oxford

See: http://nano.org.uk/euro.htm

Special funds are available to support attendance by younger scientists
(under the age of 35) from EU countries.

NOTE: Deadline for submission of abstracts: 1 August 2000.

Under the contract providing EU sponsorship, the organisers are requested
to make special efforts to attract participants from the following target
groups:

- Researchers aged 35 years or under at the time of the conference.
- Researchers whose place of work is in a less-favoured region.
- Women researchers.
- Researchers who work in industry.

The sponsorship includes funding to facilitate participation of
researchers under the age of 35 who are citizens of EU countries. In order
to take advantage of the low cost air fares which are available for
journeys which include a Saturday night stay, the meeting is being
organised over a "long weekend" (Saturday to Tuesday). Only the lowest
cost air fares can be reimbursed, within the available funds.

INTERNATIONAL STEERING COMMITEE
Professor K. Schaumburg, University of Copenhagen
Professor J.T. Devreese, University of Antwerp
Professor G.D.W. Smith, University of Oxford

PARTIAL LIST OF INVITED SPEAKERS
Professor G.A.D. Briggs, Oxford University
Professor J. Chapman, University of Glasgow
Professor J.M. Cooper, University of Glasgow
Professor P.J. Dobson, Oxford University
Dr. J. Gimzewski, IBM Zurich
Dr. J.A. Liddle, Lucent Technologies
Professor R. Palmer, University of Birmingham
Professor A. Persoons, University of Leuven
Dr. A.K. Petford-Long, Oxford University
Professor J.B. Pethica, Oxford University
Dr. Rasmita Raval, University of Liverpool
Dr. T. Spiller, Hewlett Packard
Professor M. Welland, University of Cambridge

SPECIAL EVENTS
Meeting of the ORCHYD Network for Organic-Inorganic Hybrid Devices.
Meeting of the U.K. Foresight Panel Task Force on Nanotechnology
Meeting of Summer School working group 'Self Organisation of
Nanoparticles'
Workshop session on "The interaction of Organic Molecules with Surfaces".
'Consulting the Community' workshop session: "The impact of nanotechnology
on society".
Visits to nanoscience laboratories and nanotechnology spin-out companies
in the Oxford area.
Displays of books and product literature relating to nanoscience and
nanotechnology.
Nanotechnology Networks: It is hoped to announce the successful applicants
for EPSRC networks in nanotechnology, the application deadline for which
is June 2000. These will be interdisciplinary networks including the full
range of scientific and engineering disciplines. It is expected that
successful networks will be actively looking to extend their membership at
this meeting.

CONFERENCE VENUE
Somerville College is one of the constituent colleges of Oxford
University. With a superb location a few minutes walk from the city
centre, and close to the University's main science and engineering
laboratories, it provides an excellent, self-contained location for the
conference.

PUBLICATION OF PROCEEDINGS
Selected papers from the conference will, after peer review, be published
as a special issue of Materials Science and Technology. This issue will be
available for purchase by delegates at the special discounted price of £20
per copy.

CALL FOR PAPERS
Contributed papers are sought, both for oral and poster presentation, in
all areas of nanoscience and nanotechnology.
Deadline for submission: 1st August 2000.
Please state whether you wish to submit an oral presentation or a
poster. Specified Format:

* Abstracts to fit on a single A4 page (21cm wide x 29.7 cm high),
with 2cm margins to left and right, 3cm margins top and bottom.
* Use Times font, 12 point size.
* Start title on a line 3.5 cm from the top of the page.
* Put title in bold, with upper/lower case print (i.e. only initial
letters of title word capitalised).
* Put names of authors on next line, with the name of the presenting
author underlined.
* Put addresses of authors on subsequent lines.
* Leave gap of two lines before commencing text.
* Do not indent at start of paragraphs, leave gap of one line
between paragraphs.
* Put any references at end, in numerical order.
* Send abstract either in hard copy form, or by email as a Word
attachment (specify Word 97).

Send abstracts to:
Julie Hutcheon, Events and Membership Manager, The Institute of
Nanotechnology, 9, The Alpha Centre, Stirling University Innovation Park,
Stirling FK9 4NF, Scotland, UK Email: julie-at-nano.org.uk

CONFERENCE SCHEDULE
The conference starts officially at 6 p.m. on Saturday 16th September, and
ends at 4.30 p.m. on Tuesday 19th. The first meal provided is the evening
meal on the 16th, and the last meal is lunch on the 19th. Therefore the
'nightly' accommodation and meals charge refers to a 24-hour period,
beginning with the evening meal on one day, and ending with lunch on the
following day.

GRANT INFORMATION
Grants are available to assist attendance by younger scientists from the
European Union. Reimbursement can be provided for a limited number of
researchers aged 35 years or younger, who are nationals of a member state
of the European Union or of an associated state (Iceland, Liechtenstein,
Norway and Israel). EARLY REGISTRATION IS ESSENTIAL IN ORDER TO ENSURE
GRANT ASSISTANCE.

Please indicate on your registration form if you wish to apply for grant
support, and include an estimate of your expected travel costs.
Note that only the LOWEST COST return fares to Oxford can be reimbursed.

The organisers will notify you as soon as possible if your grant
application has been accepted. Funds are limited, so early application is
essential. Reimbursement can only be made retrospectively, AFTER the
conference has taken place. You must provide the conference organisers
with evidence of your citizenship, your age at the start of the
conference, and the actual expenditure incurred. Please bring with you to
the conference your passport or other original evidence of citizenship and
date of birth, plus original receipts for all expenditure incurred.

Registration and payment may be made online - see
http://www.nano.org.uk/euro.htm

REGISTRATION FEE
Industry / Government participants: £300
University participants: £200
Registered students: £100

RESIDENTS AT SOMERVILLE COLLEGE
An inclusive charge of £65 per night is payable for residential
accommodation (in single rooms) at Somerville College, together with all
main meals, morning coffee and afternoon tea.




From daemon Mon Jul 17 09:40:05 2000



From: carla_aiwohi-at-usgs.gov
Date: Mon, 17 Jul 2000 07:12:14 -0700
Subject: Denton Vacuum info.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Rick,

The phone number for Denton Vacuum is (856) 439-9100. Fax (856) 439-9111.

Sincerely,
Carla Aiwohi





From daemon Mon Jul 17 10:06:18 2000



From: Bruce Brinson :      brinson-at-cnst.rice.edu
Date: Mon, 17 Jul 2000 09:59:08 -0500
Subject: CvsD,Fees

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I disagree that uranyl acetate is fairly innocuous. Using the appropriate
dosimeters, we have measured increases of alpha, beta, and gamma radiation
over background levels with the lid of the reagent jar open. Increased
counts were still measured with the closed jar behind an alpha shield at
some distance. We ask our scientific and support staff to take some
necessary precautions for weighing uranyl acetate and other uranium salts
(wear dust mask and latex/vinyl gloves, use a shield in front of the
balance, etc.).

Of course, while the dilute stain solution poses no radiation hazard, it
should be treated as heavy metal waste for disposal purposes. These are my
own suggestions and not necessarily that of USDA.

Bruce F. Ingber, Biologist
USDA-ARS, SRRC
1100 Robert E. Lee Blvd.
New Orleans, LA 70124
(504) 286-4270 phone
(504) 286-4419 fax
bingber-at-commserver.srrc.usda.gov

-----Original Message-----
} From: jim [mailto:jim-at-proscitech.com.au]
Sent: Wednesday, July 12, 2000 8:21 AM
To: microscopy-at-sparc5.microscopy.com


Hello Microscopist & Lab Managers,
well it's old news to a lot of you but the time has come for us to try
to support service contracts with user fees. I guess we should be
grateful to have deferred this expense to our users for this long.
If we pick a rate based historical usage, all is well? I don't think
so. The old cost vs demand has to come into play. My questions to those
of you that have lived this experience:

1. What was the impact of significant increases in user fees with
respect to hours logged?
2. Did use come back to historical levels after the sticker shock wore
off?
3. Has anyone on the university level actually been able to support
their contracts & related expenses with user fees.

Your experiences & insights would be greatly appreciated.
Also I recall that there was an meeting being organized on this &
similar subjects at M&M 2000. Would the the organizers please contact
me.

thanks,
Bruce Brinson
Rice U.





From daemon Mon Jul 17 10:51:53 2000



From: E. J. McKenzie :      elizm-at-pdx.edu
Date: Mon, 17 Jul 2000 08:37:56 -0700
Subject: thanks for formaldehyde tips

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi everyone,

just to say thanks for all your formaldehyde recipes, they solved my
polymerisation problem just in time so we had plenty of formaldehyde to take
on our field-collection trip.

cheers
Elizabeth McKenzie
--------------------------------------------------
Geomicrobiology and Electron Microscopy Laboratory
Room S9 Cramer Hall
1721 SW Broadway
Portland State University
Portland
OR97201

ph:503 725 3362
fax:503 725 3025




From daemon Mon Jul 17 11:08:48 2000



From: rgriffin-at-eng.uab.edu
Date: Mon, 17 Jul 2000 10:57:06 -0500
Subject: Subsurface crack shapes and light basics

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Help!

I have a professor here that wants to determine the subsurface crack shape
in a ceramic by placing a drop of fluorescent dye-penetrant onto
indentations. The cracks are forced open after the dye is allowed to
penetrate and the pre-existing crack area will be covered with dye
penetrant. Our reference says to use external ultraviolet illumination in
an optical microscope to distinguish the pre-cracked area.

It is my understanding that we will need a mercury vapor light and a
fluorescent dye penetrant that is made for weld crack detection. Why do I
need a mercury vapor light? Where would I find out about the basics of this
technique? Will I also need some sort of filter for the microscope?

Has anyone out there done something similar? What supplies and microscope
attachments did they use?

Thanks,

Robin D. Griffin
UAB



From daemon Mon Jul 17 12:02:50 2000



From: David P. Bazett-Jones, Ph.D. :      bazett-at-ucalgary.ca
Date: Mon, 17 Jul 2000 10:55:07 -0600
Subject: Post-Doc Position

Contents Retrieved from Microscopy Listserver Archives
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Post-Doctoral Research Position
Department of Cell Biology and Anatomy
University of Calgary
Calgary, Alberta, Canada

A post-doctoral fellowship position is available to investigate
structure-function
relationships of the mammalian cell nucleus. Specifically, the goal of
the research is to determine
at high resolution the spatial relationships of transcriptionally active
genes with sub-nuclear
domains, and how particular gene families are recruited to these
domains. The research will
involve dynamic fluorescence microscopy, digital confocal microscopy and
energy filtered
transmission electron microscopy, together with traditional biochemical
and molecular biological
approaches. Interested applicants should refer to the following recent
publications: Kruhlak et al
(2000) J. Cell Biol. 150, 41-51; Boisvert et al (2000) J. Cell Biol.
148, 283-292; Hendzel et al
(1998) Mol. Biol. Cell 9, 2491-2507.
The position includes salary, benefits and the opportunity to work
in a well-equipped and
dynamic environment in the Cancer Biology Research Group.
Send C.V. and the names of three references to:
Dr. David P. Bazett-Jones
Department of Cell Biology and Anatomy
3330 Hospital Dr
Calgary, AB T2N 4N1 Canada
TEL: (403) 220-3025, FAX (403) 270-0737
email bazett-at-ucalgary.ca




From daemon Mon Jul 17 12:25:17 2000



From: Bruce Brinson :      brinson-at-cnst.rice.edu
Date: Mon, 17 Jul 2000 12:19:25 -0500
Subject: Re: CvsD,Fees

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Lou,
Slow down... Trying to look good? Obviously something has been lost in the
translation & someone has given you the shaft in the past. I am looking for a
solution to things beyond my control. I Personally have no control over the
rates unless I can produce a solution (like yourself?). I view my self as a
(under paid) scientist & I am very pissed off at our university's attitude
toward supporting research facilities. I live eat & breath research.
We drag in mega bucks for tools & nothing for staff or support. I am hoping
to confirm by the voices of experience that this is not a solution. Ya know a
defense without data doesn't go far.
FYI our past (93-2000) fees were $5/hour on state of the art TEM, SEM, AFM ...
come on whine about that! Mean while if our locals will get their bowl in a up
roar like you seem prone to do, we may get a solution.
I appreciate the sincerity of your support even though you missed the
target.

Bruce



Lou Solebello wrote:

} Do you imply keeping fees the same as in past contracts? Forget it
} guy.....that mentality is putting scientists out of work. Nothing goes down
} in prices, and the COL has increased. USA scientists are already under paid
} and over worked because of people like you who want to look good at the
} expense of others.
} ----- Original Message -----
} From: Bruce Brinson {brinson-at-cnst.rice.edu}
} To: MSA Listserver {microscopy-at-sparc5.microscopy.com}
} Sent: Monday, July 17, 2000 7:59 AM
} Subject: CvsD,Fees
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Hello Microscopist & Lab Managers,
} } well it's old news to a lot of you but the time has come for us to try
} } to support service contracts with user fees. I guess we should be
} } grateful to have deferred this expense to our users for this long.
} } If we pick a rate based historical usage, all is well? I don't think
} } so. The old cost vs demand has to come into play. My questions to those
} } of you that have lived this experience:
} }
} } 1. What was the impact of significant increases in user fees with
} } respect to hours logged?
} } 2. Did use come back to historical levels after the sticker shock wore
} } off?
} } 3. Has anyone on the university level actually been able to support
} } their contracts & related expenses with user fees.
} }
} } Your experiences & insights would be greatly appreciated.
} } Also I recall that there was an meeting being organized on this &
} } similar subjects at M&M 2000. Would the the organizers please contact
} } me.
} }
} } thanks,
} } Bruce Brinson
} } Rice U.
} }
} }
} }




From daemon Mon Jul 17 13:28:42 2000



From: Purdy, Sam :      SPurdy-at-nationalsteel.com
Date: Mon, 17 Jul 2000 18:26:54 -0500
Subject: Re: Subsurface crack shapes and light basics

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


High pressure mercury vapour lamps are used in most fluorescence
microscopes because they are very intense sources producing
strong emissions only at UV, blue and green wavelengths. A great
many fluorescent dyes are excited by wavelengths in the visible
spectrum, and various lamps can be used to excite them if the
intensity is great enough. Fluorescein, for example can be excited
by blue light, and rhodamine by green. For many applications, e.g.
using rhodamine isothiocyanate, all you need for illumination is a
powerful source of green light, such as a tungsten halogen lamp,
and a filter which transmits green efficiently but cuts all other
wavelengths. An interference filter would be the best choice. For
viewing or photography you will need a filter which efficiently blocks
green light, but efficiently transmits orange/red. Again an
interference filter is best, but you will get a result with a red or
orange photographic filter.

For fluorescence applications at low magnification, using a macro
setup or a low power binocular microscope, and where the dye
concentrations are fairly high, black UV tubes, such as those
battery-powered hand lamps sold to read security marks can
provide enough intensity to be useful for excitation of a wide range
of fluorescent dyes. If you use these to excite rhodamine, keep the
incident light off the orange barrier filter - red, orange and yellow
dyed glass photographic filters are usually fluorescent themselves.

Chris

} From: "rgriffin-at-eng.uab.edu"-at-sparc5.microscopy.com
To: microscopy-at-sparc5.microscopy.com


Denton's web site is up and running at www.dentonvacuum.com. You can call
or fax at:
Phone: (856) 439-9100 Fax: (856) 439-9111
Thermocouple gauges are called that because they measure the conductive
heat loss from a heated filament with a thermocouple attached to the
filament. Your TEM tech is probably more familiar with Pirani gauge, which
measures the conductive heat loss by measuring the resistance of a heated
filament. The Pirani gauge is commonly used on European and Asia
instruments, where American instruments are more likely to use
thermocouples.



Allen R. Sampson, Owner
Advanced Research Systems, St. Charles, Illinois 60174
voice 630.513.7093 fax 630.513.7092

On Friday, July 14, 2000 12:48 PM,
"rlvaughn-at-unmc.edu"-at-sparc5.microscopy.com
[SMTP:"rlvaughn-at-unmc.edu"-at-sparc5.microscopy.com] wrote:
} Does any one know the current phone number and or web site of Denton
} Vacuum?
} The ones I am using : 888-336-8661, www.dentonvacuum.com are not
} responding.
} I need some advice on one of their old evaporators and possibly puchase a
} couple of thermocouples (vacuum gage). Why do they call it a
thermocouple?
} The TEM service person that was here didn't think that was a proper name.
} Thanks.
}
} Rick Vaughn
} rlvaughn-at-unmc.edu
}
}
}


From root Mon Jul 17 14:16:29 2000
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Our experience was that in 1986, when charges were first imposed
on our users, numbers of users crashed and stayed low, even when
rates of charge were insufficient to cover the bills. When charges
were raised towards realistic rates usership fell even further. Even in
today's environment, where people know that charges are a fact of
life, and that they should be raising them in grant funding, users are
rarely prepared to include funding for em in their proposals. They
want em free at the point of use, or they leave em out of the
equation, or get some of it done as a favour by someone in another
institution who doesn't apply charges.


Date sent: Mon, 17 Jul 2000 09:59:08 -0500
} From: Bruce Brinson {brinson-at-cnst.rice.edu}
Send reply to: brinson-at-rice.edu
Organization: Rice University
To: MSA Listserver {microscopy-at-sparc5.microscopy.com}


Hi!
I don't know much about the specificsof your test but you will need
a source of UV light, such as mercury lamp, to excite the fluorescing
material. Go to the Bio Dep't and talk to them about fluorescence
microscopy.
I would be very cautious about interpreting the results. Crack
penetrating fluid have very low viscosities and surface tensions in order to
penetrate the crack. Once you have broken open the specimen, I would expect
the fluid to creep over the newly generated surfaces.
A better way, I would think, would be to obtain a low viscosity
epoxy resin ( back to the Bio Dep't), add some fluorescent dye, and vacuum
impregnate the specimen. After curing the epoxy, break open the specimen.
The cracked surfaces will be covered with a solid fluorescing compound that
will not creep. A complication will be that the epoxy may cement together
the cracked surfaces and fracture will occur elsewhere.
Another approach woud be to use image analysis techniques. See Jon
Russ' book "Image Processing Handbook" or "The Image Processing Tool Kit"
from Reindeer Games, PO Box 37188, Raleigh, NC 27627.

I have no financial intersest in the company.

Sam Purdy
Technical Center,
National Steel Corp
1745 Fritz Dr, Trenton,MI 48183
spurdy-at-nationalsteel.com





From daemon Mon Jul 17 18:58:22 2000



From: sbrockett-at-tqs.com (by way of Nestor J. Zaluzec)
Date: Mon, 17 Jul 2000 18:25:30 -0500
Subject: Microtome TEM X-sections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Several years ago, we had some precision X-sections done using a microtome.
This was done to create TEM samples of gold metalization in a low-k
dielectric. Since that time, we've been unable to find the service offered
in an outside lab. Does anyone know a lab offering this type of
X-sectioning? Any info would be appreciated.


Steve Brockett
Customer Returns and Failure Analysis Manager
TriQuint Semiconductor
2300 NE Brookwood Parkway
Hillsboro, OR 97124
503-615-9303
sbrockett-at-tqs.com





From daemon Mon Jul 17 19:01:03 2000



From: Rozeveld, Steve (SJ) :      SJROZEVELD-at-dow.com
Date: Mon, 17 Jul 2000 17:07:05 -0400
Subject: used SEM, FE-SEM, TEM available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html





} Leica S 360 SEM, $35,000
} Purchased 1989, LaB6 filament.
} Cathode luminescence, Robinson BSE, Solid state BSE,
} Oxford 3 position EDS detector (BE, thin window, windowless), 4PI system for EDS & digital image acquisition.
} Buyer pays for the move. All systems are operational and available for demo.
}
} Hitachi H-600 TEM, $20,000 (without CCD camera)- $50,000 (with CCD camera)
} Purchased 1988,
} 100 keV, W filament,
} Gatan Model 791 digital camera YAG with Digital Micrograph ( 2 years old).
} Buyer pays for move. All systems are operational and available for demo.
}
} Topcon DS 130 F, $40,000
} Purchased 1989
} Schottky field emission gun SEM,
} Dual stage system
} Resolution (top stage- 10 Angstroms at 30keV, 40 Angstroms at 1keV) (bottom stage-20 Angstroms at 30keV, 100 Angstroms at 1keV), Robinson BSE on lower stage, Oxford Be window EDS detector on lower stage, 4PI system for EDS and digital image acquisition, IP30 frame over imaging system.
} Buyer pays for move. All systems are operational and available for demo.
}
}
} contact:
}
} John Blackson
} The Dow Chemical Company
} Midland, MI 48667
} 517-636-6316 office
} 517-638-6443 fax
} jhblackson-at-dow.com
}
or

} Steve Rozeveld
} The Dow Chemical Company
} Analytical Sciences Laboratory
} 1897 Bldg., Door E43
} Midland, MI 48667
} 517-636-5167 office
} 517-638-6443 fax
} sjrozeveld-at-dow.com
}



From daemon Mon Jul 17 20:25:37 2000



From: donald j marshall :      dmrelion-at-world.std.com
Date: Mon, 17 Jul 2000 21:11:25 -0400 (EDT)
Subject: new york microscopical society

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Anyone interested in the Yearbooks of the New York Microscopical Society for
1955, 1956, 1957, 1958,1961, and 1968? All or nothing. I'll even pay the
postage for domestic mailings.

Don Marshall

Donald J. Marshall
Relion Industries
P.O. Box 12
Bedford, MA 01730
Ph: 781-275-4695
FAX: 781-271-0252
email dmrelion-at-world.std.com

Cathodoluminescence, mass spectroscopy, electron beam technology


"A weed is a flower out of place."

(Please note: Do not send email with attachments to this address. Instead, send it to donbarlen-at-aol.com. Thank you.)



From daemon Mon Jul 17 21:30:16 2000



From: Smartech :      smartech-at-javanet.com
Date: Mon, 17 Jul 2000 22:27:43 -0400
Subject: Amray 1920

Contents Retrieved from Microscopy Listserver Archives
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If someone says they have an Amray 1920, what sort of SEM do they have?
Approximate year?
Fully Digital?
PC controlled?
Resolution?

Thanks
Ric






From daemon Mon Jul 17 22:01:17 2000



From: Koh YinHsian-CYK006 :      Yinhsian.Koh-at-Motorola.com
Date: Tue, 18 Jul 2000 10:52:36 +0800
Subject: Re: Subsurface crack shapes and light basics

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

Wondering if you can recommend a good penetrant and epoxy to use and where
to get it. I am in the middle to source for the penetrant to perform solder
joint crack (BGA etc), molded parts crack and plating crack. Perhpas you can
advice if this method will be the simplest method to identify the
crack/craze line.

My understand from our local supplier is they have an external accessory
that can attach to the scope. The cost of the external features is about
USD5000 (Olympus). The bulb is mercury lamp that can last only 200 hours.


Regards,
YH Koh
(MQE Engineer)
Motorola CGISS Penang,
Bayan Lepas FIZ, Phase 3,
11900 Penang, Malaysia.
Tel : 60-4-8504089
Fax: 60-4-6124903




-----Original Message-----
} From: Purdy, Sam [mailto:SPurdy-at-nationalsteel.com]
Sent: Tuesday, July 18, 2000 7:27 AM
To: Microscopy-at-sparc5.microscopy.com


Hi!
I don't know much about the specificsof your test but you will need
a source of UV light, such as mercury lamp, to excite the fluorescing
material. Go to the Bio Dep't and talk to them about fluorescence
microscopy.
I would be very cautious about interpreting the results. Crack
penetrating fluid have very low viscosities and surface tensions in order to
penetrate the crack. Once you have broken open the specimen, I would expect
the fluid to creep over the newly generated surfaces.
A better way, I would think, would be to obtain a low viscosity
epoxy resin ( back to the Bio Dep't), add some fluorescent dye, and vacuum
impregnate the specimen. After curing the epoxy, break open the specimen.
The cracked surfaces will be covered with a solid fluorescing compound that
will not creep. A complication will be that the epoxy may cement together
the cracked surfaces and fracture will occur elsewhere.
Another approach woud be to use image analysis techniques. See Jon
Russ' book "Image Processing Handbook" or "The Image Processing Tool Kit"
from Reindeer Games, PO Box 37188, Raleigh, NC 27627.

I have no financial intersest in the company.

Sam Purdy
Technical Center,
National Steel Corp
1745 Fritz Dr, Trenton,MI 48183
spurdy-at-nationalsteel.com





From daemon Tue Jul 18 02:44:35 2000



From: Rosemary White :      Rosemary.White-at-pi.csiro.au
Date: Tue, 18 Jul 2000 17:30:45 +1000
Subject: Re: CvsD,Fees

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I'll add my 2c worth...

In the past, EM/microscopy facilities I've been part of and used in
Australia have not charged fees, and most still don't charge users within
their institutions, or have a very minimal charge, e.g. $5/hour for
confocal just to put something into laser replacement, and $500-$1000 per
year for use of all EM facilities, including costs to prepare tissue, etc.
To external users, government facilities are supposed to charge commercial
rates, so they don't compete unfairly with fully commercial facilities. I
believe it's similar in the USA.

But "full cost recovery" is coming, even for users of facilities within
their own institutions. It has come to a few facilities, and the result is
a dramatic fall-off in users. Even when fairly minimal fees are imposed,
this breaks the bank for some people, especially for undergrad student
projects.

To be more specific:
1. What was the impact of significant increases in user fees with
respect to hours logged?
Hours logged for TEM dropped to 30% and continued to decline. Hours on SEM
dropped 50% and stayed low.

2. Did use come back to historical levels after the sticker shock wore
off?
No, though there was a little recovery.

3. Has anyone on the university level actually been able to support
their contracts & related expenses with user fees.
In my experience in 5 institutions, no, even when high fees were charged.

I don't know how to fight this, you need strong support from a large user
base. Even then, you can be beaten by the bean counters if they are in
ascendency.


Rosemary White
Microscopy Centre
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia

phone 61-2-6246 5475
fax 61-2-6246 5000
email r.white-at-pi.csiro.au





From daemon Tue Jul 18 05:08:14 2000



From: Kristiane Gans :      Kristiane.Gans-at-medizin.uni-magdeburg.de
Date: Tue, 18 Jul 2000 11:54:57 +0200
Subject: used microscopes in the Net

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

are ther any internetpages where I can buy/sell microscopes and
fittings?

Thanks, Kristiane




From daemon Tue Jul 18 05:42:22 2000



From: donald j marshall :      dmrelion-at-world.std.com
Date: Tue, 18 Jul 2000 06:30:26 -0400 (EDT)
Subject: Re: new york microscopical society

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ed, You were the first to reply and they are yours for the museum. Should
go out today.

Don Marshall





} From COURYHOUSE-at-aol.com Tue Jul 18 01:10:29 2000
}
} From: COURYHOUSE-at-aol.com
} Received: from COURYHOUSE-at-aol.com
}
} Date: Tue, 18 Jul 2000 01:06:41 EDT
} Subject: Re: new york microscopical society
} To: dmrelion-at-WORLD.STD.COM
}
}
} we would like to have these for the museums collection.
} many will get use of them.
} thanks Ed Sharpe archivist for SMECC
}
} Coury house / SMECC
} 5802 w Palmaire Ave
} Glendale AZ 85301
}
} { { Subj: new York microscopical society
} Date: 7/17/00 9:16:22 PM US Mountain Standard Time
} From: dmrelion-at-world.std.com (donald j marshall)
} To: Microscopy-at-sparc5.microscopy.com
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Anyone interested in the Yearbooks of the New York Microscopical Society
} 1955, 1956, 1957, 1958,1961, and 1968? All or nothing. I'll even pay the
} postage for domestic mailings.
}
} Don Marsha } }
}

Donald J. Marshall
Relion Industries
P.O. Box 12
Bedford, MA 01730
Ph: 781-275-4695
FAX: 781-271-0252
email dmrelion-at-world.std.com

Cathodoluminescence, mass spectroscopy, electron beam technology


"A weed is a flower out of place."

(Please note: Do not send email with attachments to this address. Instead, send it to donbarlen-at-aol.com. Thank you.)



From daemon Tue Jul 18 08:06:40 2000



From: Chris Edwards :      fishon-at-umich.edu
Date: Tue, 18 Jul 2000 08:55:12 -0400
Subject: Static Problems with Cryo

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


One of our researchers is having trouble with cryo-thin sections jumping
back onto the face of the block. Apparently this is a static problem.
Does anyone use an anti-static device for this? If so, what is available.
Also any thoughts for this cure would be appreciated. Thanks.





From daemon Tue Jul 18 08:11:24 2000



From: Susan Carbyn :      CarbynS-at-em.agr.ca
Date: Tue, 18 Jul 2000 09:02:39 -0400
Subject: Scale bar

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am wondering if someone could help me out with trying to use John Russ's Image toolkit for putting on scale bars for digital plates. I have light micrographs that were taken at 20 and 40x magnification, and also 20 and 40x magnification of calibration grid slides of 25 and 250 micrometers from MicrobrightField Inc. These calibration images I tried to set up a txt file using calibrate by marking out the points from one grid bar to the next, and specifying it as either 25 or 250 micrometers depending on which calibration grid I used. I saved this file and imported it on a light micrograph of similar magnification. When I do this importing, and then use the magnification bar option, the bar does not reflect the calibration scale. Also, if I just use the magnification bar on the original calibration grid, it comes out to be 7micrometers for each division rather than the 25 or 250 that I have specified. If someone could suggest where I am going wrong, I would very much appreciate it. I hope I haven't completely confused people!

Thanks,
Susan

Susan Carbyn
Electron Microscopy Technician
Atlantic Food and Horticulture Research Centre
Agriculture and Agri-Food Canada
32 Main Street, Kentville, Nova Scotia B4N 1J5
Canada

Phone: (902) 679-5566
Fax: (902) 679-2311

E-mail: carbyns-at-em.agr.ca




From daemon Tue Jul 18 08:36:37 2000



From: Brian McIntyre :      mcintyre-at-optics.rochester.edu
Date: Tue, 18 Jul 2000 09:24:00 -0400
Subject: Re: CvsD,Fees

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


hi listers-

i've been in a "cover costs or die" situation for the past 6 years. at
first usage stayed about the same...then dropped off as my user base found
cheaper and better alternatives on the equipment side of the equation (a
happier and more accomodating microscopist they'll never find!), at other
less "cost recovery" oriented universities. i was then funded by the NSF
to update some of the lab (a new FESEM in particular). with this came a new
influx of users. i'm now able to cover nearly all of the lab expenses
(contracts, supplies, upgrades, and salary). i still have a slush fund to
cover unsponsored research and classroom activities, but have imposed a
user fee for specialized training courses.

hope this helps...
b-
**************************

} Date: Mon, 17 Jul 2000 12:19:25 -0500
} From: Bruce Brinson {brinson-at-cnst.rice.edu}
} Reply-To: brinson-at-rice.edu
} Organization: Rice University
} X-Mailer: Mozilla 4.06 [en] (Win95; U)
} To: Lou Solebello {microls1297-at-mindspring.com} ,
} MSA Listserver {microscopy-at-sparc5.microscopy.com}
} Subject: Re: CvsD,Fees
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



From daemon Tue Jul 18 09:25:03 2000



From: DrJohnRuss-at-aol.com
Date: Tue, 18 Jul 2000 10:12:22 EDT
Subject: Re: Scale bar

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Susan

Are you sure that the scale is wrong? There could be two different kinds of
problems here. One is that the tool kit draws a line whose length is
determined by the size of the image, and labels it with the length in the
calibrated measurement units. This might not (would not, in general) be the
same length as the distance that you originally marked for establishing the
calibration. The other problem might be that the calibration is in fact
wrong, possibly something wrong with the file, etc., but the first
explanation is more likely. If you want to create markers that are exactly a
certain length then the best way to do it is to draw the line in a blank
image, label it as you wish, crop to just the region around the label and
line, and save it as a file. Then copy and paste this onto your images. The
calibration routine in the tool kit is primarily aimed at making measurements
come out in the right units, and the scale marker was more-or-less an
afterthought. Hope this helps and doesn't add to the confusion.

John C. Russ



From daemon Tue Jul 18 09:32:12 2000



From: jim :      jim-at-proscitech.com.au
Date: Wed, 19 Jul 2000 00:24:02 +1000
Subject: RE: safety criteria for using Uranyl Acetate

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I agree, its certainly not innocuous, but neither are many common household
goods.
The questions really are: is it more dangerous than other substances, which are
not subject to permits and would regulations and permits improve safety in any
significant way? Regulations should be relative to risks. I think its absurd
that we as a reseller are required to have permits and many other regulations
under the new Australian system.
A reseller is not opening the jar, or even the outer metal container. A 25g
glass jar of UA is enclosed in heavy Al foil (to produce low energy Al X-rays)
and then enclosed in a tin can, which shields most of the remaining radiation.
What about the end-user? Should they need a permit? To see that in perspective,
just imagine running a lab that requires a couple dozen permits for things like
OsO4, the aldehydes, cacodylic acid and many more substances including liquid
nitrogen and I suggest boiling water. You'd better hire a secretary and obtain
more funds to pay for those permits.
I have a full discussion of the topic -at-

www.proscitech.com/uranyl.htm

The other point you are making about it being a heavy metal was rebutted
recently and nicely in this forum. Gold, platinum and iridium are very heavy
metals. In fact you cannot get heavier than Iridium! You can eat these heavy
elements in great quantity if you can afford it and suffer no ill effect.
"Heavy metal" has become a slur, but its meaningless.
Uranyl acetate is fully water soluble and is not cumulative in organisms. So,
however toxic as a chemical poison (and less so radiologically), its not a
problem in the environment, unless you are emitting very high doses. Some
locations will have laws dis- allowing the discharge of tiny quantities of a 1%
UA solution; so people accumulate the material and create a problem. Much
smarter to discharge UA down the sink with plenty of water.
Please read up on our site if you are interested in the topic.
Regarding your safety precautions: I think that the people handling 200 litre
drums of uranium oxide (yellow-cake) or underground uranium miners would find
your precautions highly entertaining. Yet its these people who need to be
concerned, not laboratory staff who weigh out 0.25grams of UA once a month.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Tuesday, July 18, 2000 12:41 AM, Ingber, Bruce F.
[SMTP:bingber-at-commserver.srrc.usda.gov] wrote:
}
}
} I disagree that uranyl acetate is fairly innocuous. Using the appropriate
} dosimeters, we have measured increases of alpha, beta, and gamma radiation
} over background levels with the lid of the reagent jar open. Increased
} counts were still measured with the closed jar behind an alpha shield at
} some distance. We ask our scientific and support staff to take some
} necessary precautions for weighing uranyl acetate and other uranium salts
} (wear dust mask and latex/vinyl gloves, use a shield in front of the
} balance, etc.).
}
} Of course, while the dilute stain solution poses no radiation hazard, it
} should be treated as heavy metal waste for disposal purposes. These are my
} own suggestions and not necessarily that of USDA.
}
} Bruce F. Ingber, Biologist
} USDA-ARS, SRRC
} 1100 Robert E. Lee Blvd.
} New Orleans, LA 70124
} (504) 286-4270 phone
} (504) 286-4419 fax
} bingber-at-commserver.srrc.usda.gov
}
} -----Original Message-----
} } From: jim [mailto:jim-at-proscitech.com.au]
} Sent: Wednesday, July 12, 2000 8:21 AM
} To: microscopy-at-sparc5.microscopy.com
} Subject: want to know national permit requirements for Uranyl
} Acetate
}
} ------------------------------------------------------------------------
} The Microscopy ListServer-Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe-Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} I would also like to know of any significant incident involving UA anywhere.
} I should point out that radiation damage would not show for years and one
} would never know the original cause. The radiation though is low, so unless
} a person would take to sleeping with a jar of UA there is no real danger.
} The real danger of UA is as a non-cumulative (happily), but severe kidney
} poison, but this is of course no issue with the Radiation people.
} Cheers
} Jim Darley
}
} ProSciTech Microscopy PLUS
} PO Box 111, Thuringowa QLD 4817 Australia
} Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
} Great microscopy catalogue, 500 Links, MSDS, User Notes
} ABN: 99 724 136 560 www.proscitech.com
}




From daemon Tue Jul 18 11:05:21 2000



From: William Tivol :      tivol-at-wadsworth.org
Date: Tue, 18 Jul 2000 11:45:05 -0400
Subject: Re: Specimen and chamber contamination

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Walck, Scott D. wrote:

} 1. . . . If you want a great
} material to coat the inside of your vacuum system with that has very good
} properties, try gold. I once had a UHV system that was gold plated on the
} inside. It was also very pretty.

Dear Scott,
Maybe it's my high-voltage upbringing, but I'd be careful about
using
high-Z materials (except for apertures). The scattering and brehmsstrahlung
production cross-sections are much higher for high-Z, so stray electrons and
x-rays will be higher. This leads to high background for microanalysis and
(for sufficiently high beam energies) x-rays emerging from the column.
Yours,
Bill Tivol




From daemon Tue Jul 18 12:50:43 2000



From: Dohnalkova, Alice :      Alice.Dohnalkova-at-pnl.gov
Date: Tue, 18 Jul 2000 10:40:22 -0700
Subject: Static Problems with Cryo

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have a good experience and can recommend DiATOME Static line (this particular
one is Static line II), the ionizer with variable voltage. If you're sectioning
with a diamond cryo knife, it should really help. Let me know if you need more
info. Alice.

Alice Dohnalkova
Environmental Microbiology
Battelle, PNNL
MS P7-50
Richland, WA 99352
tel. (509) 372-0692
fax (509) 376-1321


-----Original Message-----
} From: Chris Edwards [SMTP:fishon-at-umich.edu]
Sent: Tuesday, July 18, 2000 5:55 AM
To: Microscopy-at-sparc5.microscopy.com


One of our researchers is having trouble with cryo-thin sections jumping
back onto the face of the block. Apparently this is a static problem.
Does anyone use an anti-static device for this? If so, what is available.
Also any thoughts for this cure would be appreciated. Thanks.





From daemon Tue Jul 18 15:34:32 2000



From: Carlos Kazuo Inoki :      kazuo-at-csc.albany.edu
Date: Tue, 18 Jul 2000 16:22:03 -0400 (EDT)
Subject: TEM: tripod polishing of MgO

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

Seems I am stucked with another sample for XTEM. :) I was
preparing a sample, metal thin film on MgO (100) substrate. I am having a
hard time to get it thin, monitoring the thickness with a Si piece on top.
The problem is that the MgO has a bad habit of chipping even before the Si
get thin enough to be light transparent. I observed a piece of the
substrate after ion milling in TEM and there was a network of
dislocations. Probably introduced by the polishing on diamond films. Does
someone else tried to do tripod polishing with MgO to make a wedge shaped
sample? Thanks.

Carlos

o-------------------------------------------------------o
| Carlos Kazuo Inoki |
| Department of Physics - University at Albany |
| 1400 Washington Ave.- Albany - NY - 12222 |
o-------------------------------------------------------o




From daemon Tue Jul 18 15:49:18 2000



From: Kim Pierson :      piersokw-at-uwec.edu
Date: Tue, 18 Jul 2000 15:39:28 -0500
Subject: TEM: Useful tripod polishing trick

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


A trick which I have found useful during tripod polishing is to use a 1/4"
thick mirror instead of a clear glass on the polishing wheel. The mirror
allows you to easily see if there are any contamination particles on the
surface before placing the polishing disk on the glass. I got mine made at
the local glass company for $6.50. I specifically asked them to insure that
there weren't any scratches on the surface.

Please forgive if this is common knowledge...





**************************************************************
Kim W. Pierson, Ph.D.
Dept of Physics & Astronmoy
University of Wisconsin-Eau Claire
(715) 836-5009
FAX 836-3955




From daemon Tue Jul 18 16:02:57 2000



From: JPMcC54-at-aol.com
Date: Tue, 18 Jul 2000 16:54:27 EDT
Subject: EM Virus Images Needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greetings.

Our small educational TV production company is working on a half hour video
project on VIRUSES. It is intended for classroom and library use and is for
middle school age kids. We are looking for electron microscope photographs of
various viruses including TMV, HIV, Herpes, Hepatitis B, and Rabies. We are
also interested in images showing the invasion of a bacterium by a
bacteriophage. More generic images of viruses could also be used in certain
portions of the program. Our preferred format for delivery is 35mm slides,
but we are flexible on that point. Reasonable image clarity is important,
but images can be either B&W or colorized. We can cover all expenses, and
offer a modest fee for image use. We will need a signed non-exclusive
release from the owner (broad in rights, but limited to this series), and we
can credit the appropriate person in the credits of the program. Images can
be scanned and e-mailed to us for review, or we can log on to your site to
view them. Please e-mail me at Stnehouse-at-aol.com if you would like to
discuss this further.

John McCally
Stone House Productions



From daemon Tue Jul 18 17:02:04 2000



From: Wil Bigelow :      bigelow-at-engin.umich.edu
Date: Mon, 17 Jul 2000 17:50:02 -0400
Subject: RE: Book on Vacuum

Contents Retrieved from Microscopy Listserver Archives
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TO: Chris Jeffree (and others):

I believe that my book "Vacuum Methods in Electron Microscopy" (Portland
Press, London, 1994. ISBN I85578-052-6) provides a discussion of viscous
(p. 29) and molecular (p.32) flow, and of the significance of these two
modes of flow in the overall evacuation process (Ch. 2) that can be
understood by anyone, who has had basic training in any field of science.
Dr. Audrey M. Glauert was the Editor who monitored the writing and
publication of this book, and since she is a biologist you can be sure it
can be understood by biologists.

The use of specimen anticontamination devices is also discussed (pp. 12),
as well as procedures for warming them up (p.400).

Reviewers' comments and a general description of this bok can be found at
http://pup.princeton.edu, and http://www.2spi.com/catalog/books/book48.html


Wilbur C. Bigelow, Prof. Emeritus
Materials Sci. & Engr., University of Michigan
Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu;
Fx:734-763-4788; Ph:734-662-5237





From daemon Tue Jul 18 17:46:31 2000



From: John Foust :      jfoust-at-threedee.com
Date: Tue, 18 Jul 2000 17:37:43 -0500
Subject: Zeiss OpMi-1 available

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I have a Zeiss OpMi-1 binocular tilt surgical microscope for
sale on eBay at:

http://cgi.ebay.com/aw-cgi/eBayISAPI.dll?ViewItem&item=385867802

Maddeningly, my e-mail program destroyed my microscopy mailbox
earlier today, so I lost all correspondence from several people
who were interested in this microscope.

I started the bidding at $1,500, which I thought was a reasonable
mid-point given the range of offers ($20 .. $600 .. $1,200 .. $2,000)
I'd received on it in e-mail.

- John




From daemon Tue Jul 18 17:54:54 2000



From: Joseph Passero :      jp-at-spacelab.net
Date: Tue, 18 Jul 2000 18:46:35 -0400
Subject: Available a Reichart EPI Fluorescence microscope.....

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I have available a Reichart Microstar IV EPI Fluorescence microscope in excellent condition. From
a hematology lab. Also has transmitted light

If you are interested send me a reasonable offer.

Thank You

Best Regrads

Joseph Passero

mailto:jp-at-spacelab.net



From daemon Tue Jul 18 20:21:40 2000



From: Pbgrover-at-aol.com
Date: Tue, 18 Jul 2000 21:07:22 EDT
Subject: Re: used microscopes in the Net

Contents Retrieved from Microscopy Listserver Archives
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Kristiane,

I think LabX.com is your best bet. Cheers!

Paul Grover



From daemon Tue Jul 18 21:51:08 2000



From: Gary Gaugler :      gary-at-gaugler.com
Date: Tue, 18 Jul 2000 19:38:29 -0700
Subject: Olympus PlanAPO 100X objective

Contents Retrieved from Microscopy Listserver Archives
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I have a new Olympus PlanAPO 100X/1.4 oil objective
in the container.

Will consider offers for sale; or trades for Zeiss Axioskop
Epiplan, Neo and other Axioskop and Axiotron
systems and accessories.

Cheers,
Gary Gaugler, Ph.D.

gary-at-gaugler dot com




From daemon Tue Jul 18 22:20:29 2000



From: COURYHOUSE-at-aol.com
Date: Tue, 18 Jul 2000 23:11:43 EDT
Subject: Re: new york microscopical society

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thanks much!
the collection grows
ed sharpe archivist for smecc

{ { Subj: Re: new york microscopical society
Date: 7/18/00 5:56:08 AM US Mountain Standard Time
From: dmrelion-at-world.std.com (donald j marshall)
To: COURYHOUSE-at-aol.com
CC: Microscopy-at-sparc5.microscopy.com

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America
To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
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Ed, You were the first to reply and they are yours for the museum. Should
go out today.

Don Marshall
} }



From daemon Wed Jul 19 03:09:15 2000



From: PHIL MUTCH :      Philip.Mutch-at-nottingham.ac.uk
Date: Wed, 19 Jul 2000 09:33:21 GMT0BST
Subject: Histo-technik resin

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Date sent: Mon, 17 Jul 2000 17:50:02 -0400
To: Microscopy Listserver {microscopy-at-sparc5.microscopy.com}
} From: Wil Bigelow {bigelow-at-engin.umich.edu}


Hi,

I have been asked to section undecalcified bone that has been
embedded in histo-technik resin at a section thickness up to 20 um.
Has any one any experience or know of any references, in cutting with
this medium that they would like to share eg type of
knife/microtome, optimum thickness, mounting sections onto slides and
staining.
any info would be greatly appreciated
thanks in advance

Phil
Mr Philip Mutch,
School of Biomedical Science,
E Floor Medical School,
University of Nottingham,
Nottingham,
NG7 2UH. UK.
E-mail Philip.Mutch-at-nottingham.ac.uk



From daemon Wed Jul 19 07:06:05 2000



From: Nuria Cortadellas :      nuriac-at-giga.sct.ub.es
Date: Wed, 19 Jul 2000 13:53:51 +0200 (CEST)
Subject: antibody

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Hi,
does anybody knows where I could purchase a specific cytokinin antibody?
thanks in advanced
Nuria

------------------------------------------------------------------------------
Nuria Cortadellas
Unitat de Microscopia de Transmissio
Serveis Cientifico-Tecnics
Universitat de Barcelona
C/ Sole i Sabaris, 1-3 08028 Barcelona
Tel: +34 3 402 13 52
Fax: +34 3 402 13 98
E-mail: nuriac-at-giga.sct.ub.es




From daemon Wed Jul 19 08:35:58 2000



From: hard-at-acsu.buffalo.edu
Date: Wed, 19 Jul 2000 09:25:34 -0500
Subject: Final Course Announcement

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Course Announcement

Title: Optical Microscopy and Imaging in the Biomedical Sciences

When: October 11 - October 19, 2000

Where: Marine Biology Laboratory, Woods Hole, MA, USA

Tuition: $2150 (Includes room and board, text, handouts, supplies)

Application Deadline: August 1, 2000

Admission application and information:
Carol Hamel, Admissions Coordinator
Marine Biological Laboratory
7 MBL Street
Woods Hole, MA 02543-1015
(508) 289-7401
Internet: admissions-at-mbl.edu
WWW: http://www.mbl.edu (Application forms available via Adobe Acrobat)

Course Director: Colin S. Izzard, State University of New York -at- Albany
Phone: [518] 442 - 4367
EMail: csizzard-at-csc.albany.edu

Course Description:

For Whom:
Designed primarily for research scientists, physicians, postdoctoral
trainees and advanced graduate students in animal, plant, medical and
material sciences. Non-biologists seeking a comprehensive introduction to
microscopy and video-imaging will benefit greatly from this course as well.
There are no specific prerequisites, but an understanding of the basic
principles of optics is desirable. Limited to 24 students.

The eight day course consists of lectures, laboratory demonstrations,
exercises and discussions that will enable the participant to obtain and
interpret microscope images of high quality, to perform quantitative optical

measurements, and to produce photographic and video records for
documentation
and analysis.

Topics to be covered include:
principles of microscope design and image formation
bright field, dark field, phase contrast, polarized light,
differential
interference contrast, interference reflection, and fluorescence
microscopy
confocal scanning microscopy, multiphoton excitation fluorescence
microscopy and image deconvolution
digital image restoration and 3-D reconstruction
video imaging, recording, enhancement, and intensification
analog and digital image processing and analysis
fluorescent probes and ratiometric-imaging
laser tweezers and laser scissors

Applications to live cells will be emphasized; other specimens will be
covered as well.

Students will have direct hands-on experience with state-of-the-art
microscopes, video cameras, recorders and image processing equipment
provided
by major optical and electronics companies. Instruction will be provided by
experienced staff from universities and industry.

Students are encouraged to bring their own biological (primary
cultures, cell lines, etc.) and material specimens and to discuss individual

research problems with the faculty.




From daemon Wed Jul 19 09:30:47 2000



From: William Tivol :      tivol-at-wadsworth.org
Date: Wed, 19 Jul 2000 10:15:42 -0400
Subject: Re: Formvar & 1,2 dichloroethane

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Jon Krupp wrote:

Dear Jon,

} I was just visited by one of our EH&S folks who wanted to know why I had
} 1,2 dichloroethane.
}
} Seems they track purchases and I bought some last year to make formvar films.
}
} 1,2 dichloroethane is on their bad list as a carcinogen. He was actually
} here to figure out how much might be going up the fume hood so he could
} make a report to the local air quality agency. But as we talked, it seemed
} like it would be better to not have the stuff around.

Yes, 1,2 dichloroethane is a carcinogen; it's also a hepatotoxin.
However, so is chloroform, and I don't know that there is a significant
difference in the toxicities of these two compounds. Neither is as toxic
as carbon tetrachloride.

}
} Anyone have experience with formvar in chloroform? I read it works but have
} never tried it. According to our EH&S guys, chloroform would be better than
} 1,2, dichloroethane.
}

I have used formvar in chloroform and in 1:1 chloroform-acetone;
either will work.
Yours,
Bill Tivol




From daemon Wed Jul 19 09:44:12 2000



From: William Tivol :      tivol-at-wadsworth.org
Date: Wed, 19 Jul 2000 10:28:29 -0400
Subject: Re: EDS analysis of Co in WC-Co

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Leroux christine wrote:

} I am working on powders of mixed WC and Co and I have some problems with EDS.
} EDS analysis on grains whose diffractions patterns are unambiguously
} indexed in the Co structure give a majority of W, and EDS analysis on
} grains whose diffractions patterns are unambiguously indexed in the WC
} structure (without distortion and superstructures) always indicate the
} presence of Co (with a majority of W). So I was wondering if there could be
} any problem with EDS on Cobalt (because of magnetism????)
}

Dear Christine,
The magnetic properties of Co should not affect EDS. The incoming
electrons have sufficient energies that it takes fields on the order of
kilogauss
to deflect them (typical EM lens fields). If you can get an undistorted image,
the fields in your sample will not affect the electrons' path. Because
electrons
scatter strongly, the volume of the specimen where x-rays are generated may
be much larger than you would predict from the size of the beam. Also,
scattering within the specimen area may result in parts of the specimen being
illuminated be stray electrons and producing x-rays. You do not mention
such relevant parameters as the beam and grain sizes, which lines you're
looking at, or the incident electron energy. I might be able to say more if I
knew these things.
Yours,
Bill Tivol




From daemon Wed Jul 19 09:53:08 2000



From: William Tivol :      tivol-at-wadsworth.org
Date: Wed, 19 Jul 2000 10:38:41 -0400
Subject: Re: Refrigerator osmium

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Chris Jeffree wrote:

} This doesn't seem to cover it. Gold and platinum are heavy metals.
} So are we to treat them as we treat osmium tetroxide?
} Where does light end and heavy begin anyway? There is a real
} need here for a proper definition of the risk.
}
} }
} } The problem is that any form of osmium is a heavy metal, and therefore
} } should be treated as one would cadmium or arsenic. It is not innocuous.
} }

Dear Chris, Don, etc.,
It's not the atomic mass, but the chemistry that's important.
Many
heavy elements mimic the effects of lighter--and essential--elements, so
Cd and Hg can mimic Zn, Ra can mimic Ca, and Tl can mimic K, etc. In
all these cases, the heavier element has sufficiently different properties
that it interferes with the function of something that the lighter element
is involved with. As was pointed out, OsO4 is very toxic, but Os metal
is not. In fact, Os metal has properties very much like Pt. (Again to ill-
ustrate the importance of chemistry, Pt metal is harmless, but cis-platin
is a toxic chemotherapy agent).
Yours,
Bill Tivol




From daemon Wed Jul 19 11:15:42 2000



From: Beavers, Roy :      rbeavers-at-post.cis.smu.edu
Date: Wed, 19 Jul 2000 10:49:19 -0500
Subject: Images of Zeolites

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List,

I have a customer from the chemistry department that wants to obtain some
SEM images of zeolite crystals in a flyash material (fine grain powder). My
question is how to prep this material to get it into the probe? Any ideas or
suggestions would be appreciated.

Thanks

Roy Beavers
Southern Methodist University
Dept. of Geological Sciences
Electron Microprobe Lab
P.O. Box 750395
Dallas, Tx 75275
voice: 214-768-2756
fax: 214-768-2701
E-mail: rbeavers-at-mail.smu.edu




From daemon Wed Jul 19 11:57:06 2000



From: mark.deal-at-eva-tone.com
Date: Wed, 19 Jul 2000 11:44:28 -0500
Subject: AFM - measuring pit structure of CD and DVD

Contents Retrieved from Microscopy Listserver Archives
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Hello all,

I am in need of an AFM for the purpose of measuring the pit structure of
compact disc and DVD
stampers and replicas. I am considering a Q-Scope 350 from Quesant. I am
wondering if anyone
has any experience, good or bad, with this system or anything else from this
company. Also, I am
open to other suggestions if anyone has any.

Thank you,

Mark Deal
Mastering Engineering
Eva-Tone, Inc






From daemon Wed Jul 19 12:55:33 2000



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Wed, 19 Jul 2000 12:45:58 -0500
Subject: Images of Zeolites

Contents Retrieved from Microscopy Listserver Archives
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When working with fly ash in the past, I would usually use a double-sticky
carbon tab on an SEM stub and press it down into the ash, then gently blow
off the loose particles with compressed air. Double-sided carbon tape would
probably work, although the tabs seem less likely to engulf very small
particles with excess adhesive.

Another alternative might be a product called "Mikrostik", a clear liquid
you paint onto a stub and which dries to a tacky finish. It's good for fine
particulates. It's available from Ted Pella (cat.no. 16033) and possibly
other places, as well. (No financial interest in Ted Pella here.)

A problem with fly ash is that it is difficult to avoid charging, since it's
basically a pile of spheres and irregularly shaped chunks in
not-very-continuous contact with each other. A low vacuum SEM makes this
much easier, but at the expense of high resolution. Try to blow off as many
particles as possible, so the ones left are in tight contact with the
adhesive. If that's not an option, you will have to coat your stub from
different angles, preferably on a tilting, rotating stage in the sputter
coater/vacuum evaporator.

Good luck,
Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/





-----Original Message-----
} From: Beavers, Roy [mailto:rbeavers-at-post.cis.smu.edu]
Sent: Wednesday, July 19, 2000 10:49 AM
To: 'Microscopy Listserver'; Microprobe List (E-mail)


List,

I have a customer from the chemistry department that wants to obtain some
SEM images of zeolite crystals in a flyash material (fine grain powder). My
question is how to prep this material to get it into the probe? Any ideas or
suggestions would be appreciated.

Thanks

Roy Beavers
Southern Methodist University
Dept. of Geological Sciences
Electron Microprobe Lab
P.O. Box 750395
Dallas, Tx 75275
voice: 214-768-2756
fax: 214-768-2701
E-mail: rbeavers-at-mail.smu.edu




From daemon Wed Jul 19 14:34:43 2000



From: Alfonso Goldschmidt :      agoldsch-at-cec.uchile.cl
Date: Wed, 19 Jul 2000 15:23:59 -0400
Subject: Images of Zeolites

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http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


When working with fly ash in the past, I would usually use a double-sticky
carbon tab on an SEM stub and press it down into the ash, then gently blow
off the loose particles with compressed air. Double-sided carbon tape would
probably work, although the tabs seem less likely to engulf very small
particles with excess adhesive.

Another alternative might be a product called "Mikrostik", a clear liquid
you paint onto a stub and which dries to a tacky finish. It's good for fine
particulates. It's available from Ted Pella (cat.no. 16033) and possibly
other places, as well. (No financial interest in Ted Pella here.)

A problem with fly ash is that it is difficult to avoid charging, since it's
basically a pile of spheres and irregularly shaped chunks in
not-very-continuous contact with each other. A low vacuum SEM makes this
much easier, but at the expense of high resolution. Try to blow off as many
particles as possible, so the ones left are in tight contact with the
adhesive. If that's not an option, you will have to coat your stub from
different angles, preferably on a tilting, rotating stage in the sputter
coater/vacuum evaporator.

Good luck,
Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/





-----Original Message-----
} From: Beavers, Roy [mailto:rbeavers-at-post.cis.smu.edu]
Sent: Wednesday, July 19, 2000 10:49 AM
To: 'Microscopy Listserver'; Microprobe List (E-mail)


List,

I have a customer from the chemistry department that wants to obtain some
SEM images of zeolite crystals in a flyash material (fine grain powder). My
question is how to prep this material to get it into the probe? Any ideas or
suggestions would be appreciated.

Thanks

Roy Beavers
Southern Methodist University
Dept. of Geological Sciences
Electron Microprobe Lab
P.O. Box 750395
Dallas, Tx 75275
voice: 214-768-2756
fax: 214-768-2701
E-mail: rbeavers-at-mail.smu.edu







From daemon Wed Jul 19 15:14:51 2000



From: Douglas Keene :      DRK-at-shcc.org
Date: Wed, 19 Jul 2000 12:59:36 -0700 (Pacific Daylight Time)
Subject: Coating of TEM viewing screens

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Does anyone have a positive experience getting a TEM
viewing screen re-coated? I've got a small focusing screen
for a Philips 410 , viewed with binoculars, that needs
re-coating with minimal grain size. If you have used a
vendor successfully, would you please forward their contact
information to me?

Thank you in advance,

Doug
----------------------
Douglas R. Keene
Associate Investigator
Shriners Hospital Research Facilities
3101 S.W. Sam Jackson Park Road
Portland, Oregon 97201
phone: 503-221-3434
FAX: 503-412-6894 (9-5 PST)
e-mail: DRK-at-shcc.org





From daemon Wed Jul 19 15:14:51 2000



From: Joyce Craig :      j-craig-at-csu.edu
Date: Wed, 19 Jul 2000 14:59:39 -0500
Subject: boats for 6mm knives

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I used to buy boats from LKB for 6 mm knives. Now I can't locate LKB
and the other companies seem to sell only for larger knives. Does
anyone know where I can get boats for 6 mm knives? I really don't want
to go back to the tape method.
Thanks.
Joyce Craig
Chicago State University




From daemon Wed Jul 19 15:59:26 2000



From: diane.a.ciaburri-at-gdds.com
Date: Wed, 19 Jul 2000 16:43:40 -0400
Subject: Summary - IC Deencapsulation (long)

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Here's the summary (long) for all those interested in deencapsulating plastic
encapsulated ICs. I have no prefferences as I haven't tried any yet, but
thought the fuming sulfuric acid might be 'fun'. Thanks again!

_______________________________________________________________________
********************************************************************************
********************

-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Hi Diane,

The way this is generally done is to mill the plastic down on a grinding
wheel to the point where only a fairly thin layer of plastic remains. Then,
using a plasma etcher, and a mixture of oxygen to CF4 (for example, 30%
oxygen/70% CF4), whereby the oxygen etches away the plastic and the CF4
etches away the glass frit that is usually found in the plastic, you can
remove the remaining plastic (package) without damaging the device itself.
Different people like to use different gas ratios, of course, and that is
probably a function, at least to some degree, of the concentration of glass
frit in their particular plastic.

The SPI Plasma Prep II unit, as shown on URL
http://www.2spi.com/catalog/instruments/etchers1.html in the world, is
probably the most widely used unit for doing this type of operation. It is
inexpensive and highly reliable, and requires virtually no maintenance.

Chuck
____________________________________________________________________________
********************************************************************************
***************************
The ion beam approach works well. I have not used it recently on finer pitch
ICs. With as-built feature sizes of 2-4u, it is fine. It will stop at the
passivation and leave the Al bond wires intact. The resulting package looks
like it has a V-shaped pit in it (which it does). The extent of the pit depends
on the size of the die and if you want to blast
down to the lead frame or substrate.

I have not done this on finer pitch devices. I would be a bit skeptical about
these mostly because of the smaller bond pads. The etching would still stop at
the passivation.

There are numerous places in Silicon Valley that do this on an outsource basis.
Typical costs are about $75 per IC. I can get some contacts for you if you'd
like.

gary g.
___________________________________________________________________________
********************************************************************************
*************************

Diane:  attached is a text document outlining the procedure my FA lab uses. 
Yellow fuming nitric is usually the acid of choice.   If you can get a few extra
parts to practice on, that would be best.  And you're right, plasma takes
FOREVER.    If I can be of any more help, please let me know.

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Becky Holdford  (r-holdford-at-ti.com)
972-598-1291 (pager)
KFAB Physical Analysis Labs--SEM/FIB/FA
Kilby Center West
Texas Instruments, Inc.
Dallas, TX
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~


TOOLS, EQUIPMENT, & SUPPLIES
1. Milling machine and appropriate end mills
2. Stabilo SuperFine OH pen or equivalent
3. Fume hood properly equipped for exhausting acid fumes and solvent vapors (see
reference 3.4)
4. Explosion proof hot plate (see reference 3.4)
5. Ultrasonic cleaning apparatus
6. An optical microscope capable of 100X to 500X magnification, equipped with a
lighting system.
7. Chemical resistant latex gloves
8. Chemical resistant laboratory coat
9. Chemical resistant safety glasses (see reference 3.5)
10. Hand tools (tweezers, scalpels and etc.)
11. Plastic micro-pipette
12. Fuming red nitric acid
13. Yellow nitric acid
14. Methyl alcohol
15. Acetone


1. RECORDING OF PACKAGE MARKINGS
Record all of the device markings that are on the top and bottom sides of the
devices prior to starting any of the decap operations.
2. CAVITY MILLING
Determine the exact location of the chip within the package and mark the top of
the device package showing the chip perimeter, using a Stabilo SuperFine OH pen
and a straight edge. A SAM plot or X-ray image may
be used to help determine the exact location of the chip and also to determine
the thickness of the mold compound covering the chip.
Note: This should be done on devices having large chips. Devices with small
chips (less than 0.125 inches in their longest dimension) do not require this
step.
Mill a cavity out of the plastic package that is centered over the chip. The
size of the milled cavity should typically be .050 to .100 inches larger than
the length and width dimensions of the chip. The depth of the
milled cavity depends on the thickness of the mold compound and the location and
loop height of the bond wires. During the milling operation use a vacuum line to
pick up the loose plastic particles generated.
Caution: do not mill into the bond wires or the chip. Mill counter bores on
devices with chip dimensions greater than 0.400 inches on a side. These counter
bores should be made on one or more levels within the bond pad perimeter and at
the outermost corners of the cavity (this is necessary to facilitate etching of
the mold compound at the corners of the chip before the sides are exposed and
subsequent damage to the leadframe). Care must be taken during the milling
operation to avoid excessive pressure on the mill resulting in filler induced
damage to the chip P.O. The end mill should not bind, bend, or "smoke" during
the milling operation.
3. PACKAGE ETCHING
All etching must be performed in a chemical hood that meets the requirements
defined in references #.3 and
4.Heating of acid or device prior to application of acid must be done using an
explosion proof hot plate that meets the requirements of reference #4.
Obtain the appropriate acid for use on the mold compound being removed.
Following are the acids that have been identified for the removal of the various
mold compounds.
Mold Compound Acid/Temperature
Shinetsu Red fuming nitric acid at 140-150 degrees Celsius
Plascon & Sumitomo Red fuming or yellow nitric acid 140-150 degrees Celsius
Note: Fuming sulfuric acid reacts with exposed aluminum bond pad metallization
and may result in ball bond discontinuity thus hampering further analysis.

· When using red fuming nitric acid it may be helpful to start the etching
process using a mixture of red and yellow nitric acids in order to slow down the
etch process until a "residue crust" is formed over the cavity and then switch
to the red nitric acid.
· Apply the acid in drops using a plastic micro-pipette. The drops should be
placed in the center and at the corners of the cavity in approximately a 1:1
ratio.
· Allow the acid to react with the mold compound and form a crust of dissolved
compound. Caution: Do not allow the crust to dry out completely before adding
additional drops of acid.
· Remove the dissolved material using cotton swabs or by rinsing with acetone
when the dissolved materials threaten to spill over the cavity. Caution: Rinse
the device immediately with acetone if acid
spills onto the package pins.
· Soak the device in acetone for a minimum of 10 minutes, followed by a spray of
methanol to remove loose residue and to clean the residue from the cavity rim.
· Perform a thorough microscopic inspection to determine whether all necessary
areas of the chip are exposed.
· If dried mold compound residue persists on the chip surface, use the following
in the order shown to attempt removal:
· Solvent bath (such as methyl alcohol) in ultrasonic cleaner
· Several drops of room temperature fuming sulfuric acid applied to the chip
(with the chip at room temperature) for several seconds then rinse the device in
DI water.
· Several drops of fuming sulfuric acid applied to the chip with the chip on a
100 degree Celsius hot plate. Note: Fuming sulfuric acid will attack aluminum
bond pads and is therefore the method of last resort.

_____________________________________________________________________
*********************************************************************
Can't comment on sulfuric, but I have used red fuming nitric at near boiling
temperature. Apply acid, let react. Flush witn more acid, let react, etc.

Woody White

_______________________________________________________________________
***********************************************************************

Diane,
Yes, hot fuming sulfuric and/or hot fuming nitric are used as standard
procedures for removing plastic from IC's. The process is not quite that
simple. For example, water rinses will almost certainly etch the bond pads
on the IC and thus removing connection to the outside world. Additionally,
the plastic contains fire retardants which some regions don't like being
washed down the drain. There is more detailed help through EDFAS.org (one
of ASM's branches). B&G International sells a very safe, effective etcher
which performs decapsulation automatically in minutes.

I have no association with B&G International.

David Saxon
Analytical Microscope Services
11826 Reservoir Rd. E.
Puyallup, WA 98374
253-848-7701 voice & fax
email: info-at-analyticalmicroscope.com
website: www.analyticalmicroscope.com

_________________________________________________________________________
*************************************************************************
Diane,

I used to do failure analysis on semi conductor memories which were
starting to be made of plastic/epoxy with glass rods about 20 years ago.

I have some technics and possible help but its too much to write.
Basically you drill a small hole about 0.1" deep then heat the IC on a hot
plate and then you drop your acid to remove the plastic. I dont know
chemistry, I'm and Electronics Technician. I did this work with a meterial
sicentist, my mentor.
We used fumming sulfuric acid and fuimg nitric acid, also some type of
organic pink and blue solutions to stop some of the acids etching effect.

The company back then was Burroughs Corp. today is Unysis.

I presently work for the U S Department of Energy in New York City. My
phone number is 212 6203650, I'll be happy to walk through some ideas and
things I learned.

Regards
Peter Roiz
______________________________________________________________________
**********************************************************************

Perhaps it's time to comment on this thread.

Dichloromethane and dimethylformamide are relatively effective disrupters of
most epoxies but their action is accompanied by great swelling because the
polymer becomes engorged with the liquid before any significant solvation takes
place. This will destroy wire bonds on an IC.

Fuming (essentially anhydrous) sulfuric acid acts by the completely different
process of sulfonating reactive groups that remain on the polymer. The
depolymerized and sulfonated byproducts are quite soluble not only in the acid
but usually in water as well. The worst thing that you could do in this
relatively straightforward process is to wash with water at intervals because
this would initiate almost instantaneous corrosion. It would be advisable for a
chemist, as someone trained in the handling
of reactive materials, to carry this out or at least to establish procedures and
train others with less experience. The action of sulfuric acid in this regard
is quite different than that of nitric. Nearly anhydrous nitric acid
(completely anhydrous is extremely difficult to prepare) is a very powerful
oxidizer and could lead to unstable, dangerous byproducts whereas the sulfonates
resulting from the sulfuric acid reaction are relatively stable. Water must, of
course, be prevented from splashing into any concentrated acid, especially
sulfuric.

A very strong acid such as sulfuric behaves completely differently in the
absence of water. Since most acids are highly hygroscopic and are sold as water
solutions, most people do not observe this other side of their behavior.
Without water to create an ionized electrolyte, corrosion of metals will not
take place. I have de-encapsulated ICs for failure analysis in 200 degree
sulfuric acid and been able to operate the IC
without replacing the .001" aluminum wirebonds that it came with. I recall one
instance where our company built prototype hybrid microelectronic circuits out
of such de-encapsulated ICs when their supplier was late getting a new design on
the market and the only ones available were already encapsulated.

The key is to realize that water must be excluded until the sulfonating acid has
been completely rinsed away by a non-aqueous liquid. As Mr. Saxon said, there
are simple and safe devices available for doing this operation. However, with
proper care and protective gear it can be done in a beaker on a hot plate in a
fume hood. A few ml.s of sulfuric acid are heated to drive off water until
heavy vapors are observed over the liquid (which may darken during heating due
to trace impurities). The IC is carefully lowered into the hot acid and a
vigorous reaction ensues with the epoxy
almost instantly washing into the solution. After a few seconds the IC is then
quickly lifted out and held over a receiving vessel and flooded with a stream of
ethanol. Only after this is a final rinse in deionized water carried out,
followed by fresh electronic grade ethanol and forced drying in warm air.

The ready made devices which carry out the operation are typically a small bowl
with a hinged lid from which air is withdrawn by a gentle vacuum. An inert
metal feeder tube leads from a heated reservoir for the sulfuric acid and passes
through the wall of the bowl to a position where the encapsulated device is
secured. When the lid is closed and the slight vacuum applied, the hot acid is
pulled into the bowl over the device. It is somewhat self-limiting in that, if
the lid is opened, there is no driving force to bring more acid into the
container. Naturally, the vacuum source needs to be protected by a trap and all
waste products properly handled no matter how the procedure is carried out.

John Twilley
Conservation Scientist
(formerly, Manager of the Reliability Analysis Center,
Teledyne Microelectronics)





From daemon Wed Jul 19 16:44:55 2000



From: jmkrupp-at-cats.ucsc.edu (Jon Krupp)
Date: Wed, 19 Jul 2000 14:35:46 -0700
Subject: Prelim. Announc.: EM Tech

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi:

I have been asked to pass the word on a possible upcoming EM Tech. position
at UC Santa Cruz.

The position has not yet been formalized but will involve doing biological
TEM in the lab of Yishi Jin, a recently funded Howard Hughs investigator.
Jin does serial sectioning of C. elegans and needs someone who is familiar
with the trials and tribulations of biological TEM and would be willing to
see this position as a longer term commitment.

The exact job description and salary etc. have not been set. It will
probably be a Hughs title with some EM experience required, probably not
more than a BA needed, experience and trainability might count more than
degrees.

Be aware that the cost of living in Santa Cruz is significantly higher than
most places in the US.

If you would like more information regarding this position when it finally
materializes, please send me your contact information and I will see that
you get the announcement.

Jonathan Krupp
Microscopy & Imaging Lab
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu





From daemon Wed Jul 19 18:23:14 2000



From: Christoph Wittig :      Christoph.Wittig-at-iw.uni-halle.de
Date: Thu, 20 Jul 2000 1:15:23 METDST
Subject: Re: AFM - measuring pit structure

Contents Retrieved from Microscopy Listserver Archives
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Dear Mark,

I have imaged replicas from compact discs an CD-R without any
problems using Multimode and D3000 (both in contact mode)
from di, for Measuring the dimensions of the pits (height, width...)
this technique is also very useful. Other AFM should work in
the same manner.

Hope this helps,

Christoph




From daemon Wed Jul 19 19:43:59 2000



From: John F. Mansfield :      jfmjfm-at-engin.umich.edu
Date: Wed, 19 Jul 2000 20:33:10 -0400
Subject: Hotel Rooms in Philly

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Those of you who are still waiting for accommodation in Philadelphia
and who are appalled at the room rates might like to know about the
Windsor Hotel (A Big Ten Discounted Hotel, which is how Iknow about
it). http://www.windsorhotel.com/
Rooms are $119 per night and they have availability for the week we
are at the M&M conference. Just a little info. to save a little
money!

It is 5 blocks from the convention center. Check out the map at:
http://maps.yahoo.com/py/maps.py?BFCat=&Pyt=Tmap&newFL=Use+Address+Below&addr=1700+Benjamin+Franklin+Parkway&csz=Philadelphia%2C+PA%2C+19103&country=us&Get%07Map=Get+Map
This is no worse that the Wyndham which is about 6 blocks away.

Hope this is useful to someone.

Cheers Jfm.
--

Dr. John Mansfield CPhys MInstP
North Campus Electron Microbeam Analysis Laboratory
417 SRB, University of Michigan
2455 Hayward, Ann Arbor MI 48109-2143
Phone: (734) 936-3352 FAX (734) 763-2282
Cellular Phone: (734) 358-7555
(Leaving a phone message at 936-3352 is preferable to 358-7555)
Email: jfmjfm-at-engin.umich.edu
URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html
Location: Lat. 42° 16' 48" Long. 83° 43' 48"



From daemon Thu Jul 20 04:35:02 2000



From: Robert H. Olley :      r.h.olley-at-reading.ac.uk
Date: Thu, 20 Jul 2000 10:18:04 +0100 (BST)
Subject: Explosive chloroform-acetone as alt. to dichloroethane

Contents Retrieved from Microscopy Listserver Archives
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In looking for a substitute for dichlorethane, there is another hazard.
Chloroform-acetone mixtures can be explosive, especially where even traces
of base (in the glass of the container, even) might generate
dichlorocarbene, leading to a runaway reaction leading to an explosion.
Storage in particular is out of the question, and we always make sure that
these two do not share the same waste bottle.

I asked our Head of Chemical Safety, and he tells me that the use of this
mixture was specifically discontinued round about 1975, when he was doing
his exams. I also remember reading about this in "Chemistry in Britain"
long ago, but this was certainly before 1981, which is as far back as our
literature database goes.

+------------------------------------------------------------------------+
| Robert H.Olley Phone: |
| J.J.Thomson Physical Laboratory {direct line +44 (0) 118 9318572 |
| University of Reading {University internal extension 7867 |
| Whiteknights Fax +44 (0) 118 9750203 |
| Reading RG6 6AF Email: R.H.Olley-at-reading.ac.uk |
| England URL: http://www.reading.ac.uk/~spsolley |
+------------------------------------------------------------------------+






From daemon Thu Jul 20 05:37:18 2000



From: Loukie Adlem :      ladlem-at-csir.co.za
Date: Thu, 20 Jul 2000 12:25:24 +0200
Subject: SEM/EDS: performance monitoring

Contents Retrieved from Microscopy Listserver Archives
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Hi Collegues

For our quality system, I need to get a process going whereby I can monitor the performance of our SEM/EDS system over a period of time. Things that I am doing are e.g. resolution tests, checking the EDS calibration and so on.

I would really appreciate input from you and methods/checks you have found that work.

If this has been discussed before, maybe give me a pointer to when, so I can search the archives more effectively.

Regards
Loukie






Loukie Adlem
CSIR-NML
Tel: +27 012 841 4270
Fax: +27 012 841 2646
ladlem-at-csir.co.za
http://www.nml.csir.co.za





From daemon Thu Jul 20 06:49:16 2000



From: Jeannie Weakliem :      jeannie-at-pgt.com
Date: Fri, 21 Jul 2000 08:23:44 -0400
Subject: RE: Hotel Rooms in Philly

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


LKB was eaten by Leica. But the Trufs are still available through
your Leica dealer. 3 sizes, I believe.

See also Pelco GKB for 6.4 mm glass from Ted Pella's web site
http://www.tedpella.com/glass_html/glassacc.htm

PELCO® Glass Knife Boat GKB
123-3 pkg/100 usd14.00
123-3 pkg/300 usd36.12

Date sent: Wed, 19 Jul 2000 14:59:39 -0500
} From: Joyce Craig {j-craig-at-csu.edu}
Send reply to: j-craig-at-csu.edu
Organization: Chicago State University
To: microscopy listserver {microscopy-at-sparc5.microscopy.com}


Another option for hotels in Philly is the Hilton Garden Center located at
the convention center. I was able to get rates of $104/night through
travelocity.



The Microscopy Society of America


Those of you who are still waiting for accommodation in Philadelphia
and who are appalled at the room rates might like to know about the
Windsor Hotel (A Big Ten Discounted Hotel, which is how Iknow about
it). http://www.windsorhotel.com/
Rooms are $119 per night and they have availability for the week we
are at the M&M conference. Just a little info. to save a little
money!

It is 5 blocks from the convention center. Check out the map at:
http://maps.yahoo.com/py/maps.py?BFCat=&Pyt=Tmap&newFL=Use+Address+Below
&addr=1700+Benjamin+Franklin+Parkway&csz=Philadelphia%2C+PA%2C+19103&cou
ntry=us&Get%07Map=Get+Map
This is no worse that the Wyndham which is about 6 blocks away.

Hope this is useful to someone.

Cheers Jfm.
--

Dr. John Mansfield CPhys MInstP
North Campus Electron Microbeam Analysis Laboratory
417 SRB, University of Michigan
2455 Hayward, Ann Arbor MI 48109-2143
Phone: (734) 936-3352 FAX (734) 763-2282
Cellular Phone: (734) 358-7555
(Leaving a phone message at 936-3352 is preferable to 358-7555)
Email: jfmjfm-at-engin.umich.edu
URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html
Location: Lat. 42? 16' 48" Long. 83? 43' 48"




From daemon Thu Jul 20 08:06:44 2000



From: Gokula Kannan :      gkanna01-at-bcuc.ac.uk
Date: Thu, 20 Jul 2000 07:52:28 -0500
Subject: listserver enquiry

Contents Retrieved from Microscopy Listserver Archives
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Dear All,
I am a first year Ph.D student in Entomology at FPRC, Buckinghamshire
college, UK. My project is got to compare the gut structure of several
Cerambycid woodborers and their relevance in taxonomy.
I have given some of the wood borers which I am gonna work with. Can any
one tell me of which fixative( with its chemical constituents) will be
the best for me to use on for each beetles in order to get best
results?????

Hylecoetus dermestoides
Lymexylon navale
(The above two species belongs to Lymexylidae but are closely related
to the
family cleridae).
Nacerdes melanura (oedemeridae), Rhagonycha fulva (oedemeridae?),
Phymatodes testaceus
Hylotrupes bajulus , Stromatium barbatum ( both species belongs to
cerambycidae-subfamily: cerambycinae).

Thanks,

Yours truly,
Gokula kannan.





From daemon Thu Jul 20 08:07:25 2000



From: Mark Sanders :      msanders-at-biosci.cbs.umn.edu
Date: Thu, 20 Jul 2000 07:54:34 -0500
Subject: alginate stain

Contents Retrieved from Microscopy Listserver Archives
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Does anyone know of a fluorescent or light microscope stain for alginate?
Thanks in advance.
MS






From daemon Thu Jul 20 08:26:41 2000



From: Karen Rethoret :      rethoret-at-yorku.ca
Date: Thu, 20 Jul 2000 09:16:37 -0400 (EDT)
Subject: Carbon evaporator req'd Toronto Area

Contents Retrieved from Microscopy Listserver Archives
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We urgently require a carbon evaporator capable of shadowing. If anyone in
this area has surplus equipment they would like to sell/unload, please
contact me off-line.

Thanks,

Karen Rethoret
Biology Department
Microscopy Laboratory
York University, Toronto
416-736-2100 x33289





From daemon Thu Jul 20 09:30:59 2000



From: diane.a.ciaburri-at-gdds.com
Date: 7/17/00 10:57 AM
Subject: Subsurface crack shapes and light basics

Contents Retrieved from Microscopy Listserver Archives
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Robin,

I've done this before and it works very well. It is important to make sure that
the dye is completely dry before opening the crack. Otherwise, the dye will
creep across your surface of interest (yes, I've done that).

The materials we've used are from Magnaflux's Zyglo line: www.magnaflux.com

They can probably help with selection of materials and drying requirements.

These chemicals are typically used for liquid penetrant testing of materials as
a nondestructive method of finding cracks and surface discontinuities. The
general procedure includes 1)surface cleaning, 2)application of the dye for a
prescribed period of time, 3)cleaning the dye from the surface (but not out of
cracks), 4)applying a 'developer' to the surface to draw the dye out of the
cracks, and 5)examination under a black light.

Since you want to look at the opened cracks, the developer step probably won't
be appropriate.

The dye has a low viscosity and low surface tension so it will creep into cracks
well, but to improve on this, you might want to pressurize a submersed specimen
to about 2 atm. Or possibly drawing a vacuum on a submersed specimen would pull
out bubbles trapped in the cracks.

The light used for fluorescent liquid penetrant testing is typically a black
light consisting of a current regulating transformer, a mercury arc bulb and a
deep red-purple filter (for safety and to pass appropriate wavelengths). This
is a fairly common type of industrial black light. The light is supposed to
produce an intensity of at least 800 microwatts per square cm at the test
surface. I use one of these black lights with my stereomicroscope (not attached
to the microscope - just next to it).

Hope this helps.

Diane Ciaburri
General Dynamics
Pittsfield MA 01210
diane.a.ciaburri-at-gdds.com
(413)494-2847


____________________Reply Separator____________________

Help!

I have a professor here that wants to determine the subsurface crack shape
in a ceramic by placing a drop of fluorescent dye-penetrant onto
indentations. The cracks are forced open after the dye is allowed to
penetrate and the pre-existing crack area will be covered with dye
penetrant. Our reference says to use external ultraviolet illumination in
an optical microscope to distinguish the pre-cracked area.

It is my understanding that we will need a mercury vapor light and a
fluorescent dye penetrant that is made for weld crack detection. Why do I
need a mercury vapor light? Where would I find out about the basics of this
technique? Will I also need some sort of filter for the microscope?

Has anyone out there done something similar? What supplies and microscope
attachments did they use?

Thanks,

Robin D. Griffin
UAB




From daemon Thu Jul 20 10:29:02 2000



From: William Tivol :      tivol-at-wadsworth.org
Date: Thu, 20 Jul 2000 11:08:57 -0400
Subject: Re: Formvar & 1,2 dichloroethane

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Sender: tivol-at-wadsworth.org
Message-ID: {39771609.BC8579A5-at-wadsworth.org}


Warren E Straszheim wrote:

} On the side, I had a chemist tell me some years back that the reason that
} chloroform got a bad rap was because of the minute amounts of carbon
} tetrachloride that were almost inevitably present. He said chloroform was
} not so bad in its purest form. Any comments?
}

Dear Warren,
I looked up the three compounds--CCl4, CHCl3, and (CH2Cl)2--
in the Merck Index (11th Edition). CCl4 is the worst: "Poisoning by
inhalation, ingestion or skin absorption", followed by a list of nasty
symptoms; CHCl3 is not nearly as bad; however, "Inhalation of large
doses may cause hypotension, respiratory and myocardial depression
and death. Banned by FDA from use in drug, cosmetic and food pack-
aging products in 1976.", although it was in use in cough syrups and
throat lozenges before that; (CH2Cl)2 does not have a section on Human
Toxicity, but under Caution, "Vapors may produce irritation of respira-
tory tract and conjunctiva, corneal clouding, CNS depression, liver and
kidney impairment." All three "may reasonably be anticipated to be
carcinogenic." The 12th edition lists fewer symptoms for (CH2Cl)2
and CHCl3 and does not have a Human Toxicity section for CCl4,
although the same quotation and nasty symptoms are listed in the
Caution section.
I'm sure from the above that CCl4 impurities in CHCl3 would
make it much more toxic, but CHCl3 is not innocuous. Of course, all
these compounds should be used in a hood.
Yours,
Bill Tivol





From daemon Thu Jul 20 11:06:26 2000



From: William Tivol :      tivol-at-wadsworth.org
Date: Thu, 20 Jul 2000 11:45:51 -0400
Subject: Re: SEM/EDS: performance monitoring

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Loukie Adlem wrote:

} For our quality system, I need to get a process going whereby I can monitor the performance of our SEM/EDS system over a period of time. Things that I am doing are e.g. resolution tests, checking the EDS calibration and so on.
}
} I would really appreciate input from you and methods/checks you have found that work.
}

Dear Loukie,
Resolution tests for Si(Li) detectors--the usual ~145 eV resolution
EDS detector--are done with the Mn K-alpha line, so evaporating Mn on
a formvar grid, placing it in the scope, and measuring the FWHM of the
peak will give you the resolution of your system. Using a 55Fe source with
the detector not mounted in the scope will test the resolution of the crystal
apart from noise introduced by the scope, e-beam, etc. If these two mea-
surements differ, and the resolution from the latter measurement is OK,
then the detector is OK, but something in the probe system is lowering the
resolution. I use Al evaporated onto a formvar-coated Cu grid for calibra-
tion. I set up the conditions such that I get roughly equal count rates from
Al and Cu K-alpha lines by putting the beam near a grid bar and tweaking
the appropriate adjustments on the analyser according to the manual.
The software for all these measurements was included in the
analyser we bought nearly 20 years ago, and, I'm sure, is included in every
system now on the market. These checks take ~10 min each at most.
Yours,
Bill Tivol




From daemon Thu Jul 20 11:40:36 2000



From: Winton Cornell :      winton-cornell-at-utulsa.edu
Date: Thu, 20 Jul 2000 11:20:01 -0500
Subject: distinguishing polymer beads and "attached" microorganisms - HOW?

Contents Retrieved from Microscopy Listserver Archives
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Microscopists:

A post-doc in our Chemical Engineering department came to me with
this need - to quote:

"Is there a way to distinguish between white polymer "buds" and
microorganisms in a black-and-white SEM picture at a magnification of
8000X ? Both the cultured "bugs" and "buds" have the same size,
shape, and appearance in/on spherical carbon/polymer beads that are
sliced in half, fixed, mounted, and then viewed under the SEM".

As you can see, it would appear that the polymer beads have surface
textures/features ("buds") that are of comparable "shape" and "size"
to the microorganisms cultured on the surface of the beads. I have
yet to see the photos, so I can not comment on just what he is, or is
not, seeing. The sample prep, as I understand it (never having done
it!):

1. fixation in 1% glutaraldehyde with 0.1 molar cacodylate (excuse
any misspellings)
2. post-fix of 1% osmium tetroxide in 0.1 molar cacodylate
3. dehydration in ethanol
4. critical point drying
5. sputter coating

(whew!.....a lot more complicated than powder on a carbon sticky tab!!)

So, based on this information, can any of you suggest a/other
(pre-SEM) processing technique(s) (stains?) that he and our
microscopist (in the Biology department) might utilize to help
"differentiate" these "buds" from the cultured microorganisms?

Winton

PS: you can direct your feedback to me or the listserv - in either
case I will compile the responses and have them available should any
of you out there have need for them

PS #2: I have an SEM too, with a Be-windowed EDX detector (= no
light-element detection)


--

Winton C. Cornell, Ph.D.
Department of Geosciences
The University of Tulsa
Tulsa, OK 74104
phone: 918-631-3248
fax: 918-631-2091

e-mail: winton-cornell-at-utulsa.edu




From daemon Thu Jul 20 11:43:29 2000



From: wft03-at-health.state.ny.us
Date: Thu, 20 Jul 2000 12:28:01 -0400
Subject: Re: Coating of TEM viewing screens

Contents Retrieved from Microscopy Listserver Archives
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Does anyone have a positive experience getting a TEM
viewing screen re-coated? I've got a small focusing screen
for a Philips 410 , viewed with binoculars, that needs
re-coating with minimal grain size. If you have used a
vendor successfully, would you please forward their contact
information to me?


Dear Doug,

Some years ago we had a very good experience with Grant

Scientific. We talked to Dana Dunkelberger; his phone # then was

(803) 892-2841--I'd make it better than even that the area code

has been changed. We sent him blanks for both standard high-

brightness and high-resolution focussing screens and have been

using them ever since. The quality is excellent and the prices were

OK at several hundred dollars each screen. I also have a procedure

we have used to coat screens ourselves. Other than as a satisfied

customer, I have no relationship with Grant Scientific.

Yours,

Bill Tivol





From daemon Thu Jul 20 11:59:09 2000



From: John F. Mansfield :      jfmjfm-at-engin.umich.edu
Date: Thu, 20 Jul 2000 12:54:09 -0400
Subject: Ion Miller Service

Contents Retrieved from Microscopy Listserver Archives
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} From: "Tindall, Randy D." {TindallR-at-missouri.edu}
To: "'Beavers, Roy'" {rbeavers-at-post.cis.smu.edu}
Copies to: "'microscopy-at-sparc5.microscopy.com'"
{microscopy-at-sparc5.microscopy.com}


Please reply to: kimwin-at-eecs.umich.edu

A colleague of mine (Kim Winnick) has a ion miller that needs service
and I was wondering if anyone knows of any service organization that
could help him out.

Ion Mill System Description:
Manufactured by CVC
(Commonwealth Scientific Corp):
Millatron IV G Ion Beam Source
with a Millatron 400 Power Supply,
an ID-3500 Beam Controller and
a Single Wafer Load Lock Assembly.

ADVThanksANCE

Cheers
Jfm.

--

Dr. John Mansfield CPhys MInstP
North Campus Electron Microbeam Analysis Laboratory
417 SRB, University of Michigan
2455 Hayward, Ann Arbor MI 48109-2143
Phone: (734) 936-3352 FAX (734) 763-2282
Cellular Phone: (734) 358-7555
(Leaving a phone message at 936-3352 is preferable to 358-7555)
Email: jfmjfm-at-engin.umich.edu
URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html
Location: Lat. 42° 16' 48" Long. 83° 43' 48"



From daemon Thu Jul 20 13:01:52 2000



From: Steven Slap :      SSlap-at-ebsciences.com
Date: Thu, 20 Jul 2000 13:49:27 -0400
Subject: boats for 6mm knives

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Joyce and fellow microscopists,

LKB is now part of Leica and the 6mm (actually, they're 6.4mm) boats or
"Trufs" are available from Leica distributors like ourselves in cases of
500, part #16840042.

Best regards,
Steven Slap

******************************
Energy Beam Sciences, Inc.
The Laboratory Microwave Company
http://www.ebsciences.com
Adding Brilliance to Your Vision
******************************

-----Original Message-----
} From: Joyce Craig [SMTP:j-craig-at-csu.edu]
Sent: Wednesday, July 19, 2000 4:00 PM
To: microscopy listserver


I used to buy boats from LKB for 6 mm knives. Now I can't locate LKB
and the other companies seem to sell only for larger knives. Does
anyone know where I can get boats for 6 mm knives? I really don't want
to go back to the tape method.
Thanks.
Joyce Craig
Chicago State University





From daemon Thu Jul 20 13:45:26 2000



From: Thomas, Tami/SEA :      tthomas-at-ch2m.com
Date: Thu, 20 Jul 2000 12:33:08 -0600
Subject: SEM/EDX Mapping - What is accuracy and why does area% not equal 1

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am a student with a great deal of SEM-EDX mapping data that was obtained
on an Amray 1830 SEM with a Noran Pioneer Series II x-ray analyzer with
micro-Z detector. I am trying to use this data to look at the differences
between uranium precipitates obtained in a batch study with apatite
(Ca5(PO4)3(F,OH)) and potassium. I'm getting interesting trends based on
the area% association of U-Ca-K-P and pH. However, there is a huge
difference between the sum of all of the area%s reported for a given sample
(which includes a "no element" area%). The total summed area % for these
sample/scans ranges from 20% to 87%. The elemental analyses of these
precipitates indicated that most (97 to 99.9 wt%) of any given sample was
made up of U, K, Ca, and P.
Can anyone explain to me what it means when the total area% of all of the
possible associations mapped sums to much less than 100%? What are the
possible differences between samples or scans that sum to near 100% and
those that sum to 20%?
Tami Thomas - tthomas-at-ch2m.com -



From daemon Thu Jul 20 15:38:12 2000



From: Caroline Schooley :      schooley-at-mcn.org
Date: Wed, 20 Sep 2000 13:26:08 -0700
Subject: Micrographs needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Please reply directly;Somayyah is not on this listserver.

} Fabian-Baber is a small production and communication company in Primos,
} Pennsylvania that creates educational materials. We are currently producing
} a ten-part video series for middle school children on the human body, which
} includes segments such as The Brain & Nervous System, Cells, Genes &
} Heredity, The Immune System, etc. We have just begun an arduous search for
} a broad variety of interesting, creative, and dynamic images and animations
} of the inside of the human body.
} One of the things we are looking for are images using a variety of
} microscopy techniques, both still and moving, of any aspect of the human
} body. As we are a small company which produces only educational material,
} our budget is fairly modest, however we still strive to produce high quality
} material. Thus we are looking for images donated by researchers and
} research institutions. Donors of course will be acknowledged in the
} credits. If necessary, we do have some funds allotted for obtaining images
} and footage for this series.
} If you are interested in helping this project or learning more about it,
} please get in touch with me, Somayyah, at the contact listed below. I would
} be most appreciative of any assistance you could offer. Thank you in
} advance for your time and attention.
}
} Somayyah Siddiqi
} Fabian-Baber Communications, Inc.
} 525 Mildred Ave.
} Primos, PA 19018
} e-mail: sam-at-fabian-baber.com
} phone: 610 623 7812
} fax: 610 623 8970

Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html





From daemon Thu Jul 20 18:29:54 2000



From: richard black :      m02jmy00-at-cwcom.net
Date: Fri, 21 Jul 2000 00:12:50 +0100
Subject: Film Scanners

Contents Retrieved from Microscopy Listserver Archives
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Hello,
Can anyone out there help me. I am not sure if this is the right forum
for this question.
I am still looking for a film scanner for 4X5 film. The work is mainly
technical macro work in the magnification range 1X to 50X, the subject
matter being mainly aero engine parts. We have decided that we do not
need more than about 1000dpi as most of our scans will be at about
300dpi as the prints are for reports and are rarely enlarged. So far I
have seen good reports on the Microtek and the Agfa Duoscan. Any
comments and also any comments on others such as the Umax Powerlook 3 or
the Epson would be welcome.
Thanks in advance for any help.
Cheers Richard Black




From daemon Thu Jul 20 18:30:27 2000



From: cresponc-at-moffitt.usf.edu ()
Date: Thu, 20 Jul 2000 18:17:34 -0500
Subject: fluorescence microscopy course?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Email: cresponc-at-moffitt.usf.edu
Name: Nichole Crespo
School: University of South Florida

Question: I have a little experience with fluorescence microscopy and am
looking for a good course (a week or so) on fluorescent microscopy
techniques (fixation, confocal microscopy, etc.) Know of any?

Thanks!

---------------------------------------------------------------------------





From daemon Thu Jul 20 19:35:28 2000



From: Kristen Lennon :      kalen-at-citrus.ucr.edu
Date: Thu, 20 Jul 2000 17:25:45 -0700
Subject: fluorescence mounting medium

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Knowledgeable Ones,
A few weeks ago I sent in a question regarding problems a colleague of
mine had been having with Vectashield and autofluorescence on tissue that
had been labeled with a secondary antibody conjugated to FITC, and received
many emails of support and suggestions. For anyone curious, the theme was
two part. The first thing to try was a new mounting medium. Secondly a
different fluorophore (especially favoured was Alexa 488). Thanks to all of
you who responded.
Here's the question. We decided to try a different mounting medium,
specifically Citifluor. Moments before I sat down to write this, there was
a small scream of exasperation from the microscope area as my colleague
noticed that what she had just used was Citifluor non-fluorescent immersion
oil. We quickly looked up its specifications in the catalog, and its
usefulness in this application is still nebulous. Does anyone have any
thoughts/advice?
Thanks again,
Kristen
Kristen A. Lennon
Cell, Molecular & Developmental Biology Group
Department of Botany & Plant Sciences
University of California
Riverside, CA 92521
kalen-at-citrus.ucr.edu




From daemon Thu Jul 20 21:28:18 2000



From: Djemge-at-aol.com
Date: Thu, 20 Jul 2000 22:15:01 EDT
Subject: subscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html






From daemon Thu Jul 20 21:53:43 2000



From: jim :      jim-at-proscitech.com.au
Date: Fri, 21 Jul 2000 11:39:32 +1000
Subject: RE: SEM/EDX Mapping - What is accuracy and why does area% not equal 1 00?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


There is a complex interaction between the beam and the specimen. Usually
standardisation is performed in spot mode or by simply selecting the highest
magnification buttons. If you repeat the standardisation on a homogeneous
standard using a lower magnification your results will be different to a spot
analyses. There are many reasons including: the spatial resolution for your
atomic number specimen (using a spot) will be several micrometers in area, so
the area analysed is not the actual. Furthermore, the beam deflection system
does not necessarily deliver the same number of electrons at various
magnifications, there will for instances be different losses at the apertures.
To do area analyses you would at least need to also standardise using the same
area.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Friday, July 21, 2000 4:33 AM, Thomas, Tami/SEA [SMTP:tthomas-at-ch2m.com]
wrote:
}
}
} I am a student with a great deal of SEM-EDX mapping data that was obtained
} on an Amray 1830 SEM with a Noran Pioneer Series II x-ray analyzer with
} micro-Z detector. I am trying to use this data to look at the differences
} between uranium precipitates obtained in a batch study with apatite
} (Ca5(PO4)3(F,OH)) and potassium. I'm getting interesting trends based on
} the area% association of U-Ca-K-P and pH. However, there is a huge
} difference between the sum of all of the area%s reported for a given sample
} (which includes a "no element" area%). The total summed area % for these
} sample/scans ranges from 20% to 87%. The elemental analyses of these
} precipitates indicated that most (97 to 99.9 wt%) of any given sample was
} made up of U, K, Ca, and P.
} Can anyone explain to me what it means when the total area% of all of the
} possible associations mapped sums to much less than 100%? What are the
} possible differences between samples or scans that sum to near 100% and
} those that sum to 20%?
} Tami Thomas - tthomas-at-ch2m.com -




From daemon Fri Jul 21 05:33:25 2000



From: Gordon Couger :      gcouger-at-couger.com
Date: Fri, 21 Jul 2000 04:04:41 -0500
Subject: Ramam Spectrometer

Contents Retrieved from Microscopy Listserver Archives
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This equipment in almost new condition. If some one needs this it is in
vary good shape.

I was helping Bob Taig sort out a Raman Spectrometer. It uses a 4 or 5
watt laser(tunable I think but I am not sure) and a frequency doubler
through a 1 meter long 4 reflector monocromater with a cooled detector and
cooled photomultliplier. It uses an Olympus BH2 microscope with
transparent and opaque lighting and 100 X.95, 50x.80 and 5X.13 Plan
objectives. The microscope has 4 stages and a flip top 1.3 condenser.

It was removed from a working lab when the entire lab was dismantled. It
is in almost new condition. The plastic covering on the microscope stage
is still attached and almost perfect. This is normally discarded at
installation. It was billed in February of 1988 for a little over a
quarter million dollars.

It is marked Yobin Yvon and Mole systems.

It appears to work by sending the laser into the monocromater and into the
microscope and the light is reflected off the sample back out the same
path as it came in and is split off in the monocromater to the detector.

It a appears all MSDOS computers, software and documentation is intact.
The only thing we could find missing was the video camera that mounted on
the microscope and the table that held the monocromater and microscope
above the laser and the mirrors and lenses that manipulated the light from
what came out of the laser to what the moncromater needed.. The Microscope
is set up to feed directly into a video camera. A binocular head could be
used if some one wanted to set it manually insted of remotely.

There is a lot of very nice equipment here if someone has a use for it. It
has some very nice hardware for a optical bench.

If you are interested contact Bob Taig : 580-765-9727
ustrade-at-fullnet.net The equipment is located at Ponca City Oklahoma.

I have some pictures I will have up tomorrow at
http://www.couger.com/gcouger/raman/

Gordon
Gordon Couger gcouger-at-couger.com
Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00







From daemon Fri Jul 21 08:19:06 2000



From: Huggins, Bradley J :      HUGGINBJ-at-bp.com
Date: Fri, 21 Jul 2000 08:06:28 -0500
Subject: RE: Images of Zeolites

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Randy and Kent
I also use the double sticky (conductive) tape procedure to mount zeolites
and fly ash specimens. The only additional caution I would add, is to avoid
blowing the specimen across the stub. Because of the significant difference
in shape/size of the zeolite crystals and the fly ash agglomerates, you may
change the distribution of the particles in the specimen that you examine.
For example, if bigger less dense flocs of fly ash exist, they may readily
blow away. Rather than blowing onto the stub, I try to get several
representative micro samplings and disperse those across the tape surface,
and then blow away the excess to achieve a "mono-layer" of particles.
Good Luck,
Brad

} ----------
} From: Kent Hampton[SMTP:khampton-at-oz.oznet.ksu.edu]
} Sent: Thursday, July 20, 2000 11:44 AM
} To: Tindall, Randy D.
} Cc: 'microscopy-at-sparc5.microscopy.com'
} Subject: RE: Images of Zeolites
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} } From: "Tindall, Randy D." {TindallR-at-missouri.edu}
} To: "'Beavers, Roy'" {rbeavers-at-post.cis.smu.edu}
} Copies to: "'microscopy-at-sparc5.microscopy.com'"
} {microscopy-at-sparc5.microscopy.com}
} Subject: RE: Images of Zeolites
} Date sent: Wed, 19 Jul 2000 12:45:58 -0500
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} Here, we routinely work with starches and do so in a similar
} manner. Regular double sided tapes works fine for us. To help get
} some area with a "mono-layer" of particles we use a spatula to
} place a small pile of the powder on one edge on the stub and then
} blow across the stub spreading the powder.
} }
} } When working with fly ash in the past, I would usually use a
} double-sticky
} } carbon tab on an SEM stub and press it down into the ash, then gently
} blow
} } off the loose particles with compressed air. Double-sided carbon tape
} would
} } probably work, although the tabs seem less likely to engulf very small
} } particles with excess adhesive.
} }
} } Another alternative might be a product called "Mikrostik", a clear
} liquid
} } you paint onto a stub and which dries to a tacky finish. It's good for
} fine
} } particulates. It's available from Ted Pella (cat.no. 16033) and
} possibly
} } other places, as well. (No financial interest in Ted Pella here.)
} }
} } A problem with fly ash is that it is difficult to avoid charging, since
} it's
} } basically a pile of spheres and irregularly shaped chunks in
} } not-very-continuous contact with each other. A low vacuum SEM makes
} this
} } much easier, but at the expense of high resolution. Try to blow off as
} many
} } particles as possible, so the ones left are in tight contact with the
} } adhesive. If that's not an option, you will have to coat your stub from
} } different angles, preferably on a tilting, rotating stage in the sputter
} } coater/vacuum evaporator.
} }
} } Good luck,
} } Randy
} }
} } Randy Tindall
} } EM Specialist
} } Electron Microscopy Core Facility
} } W122 Veterinary Medicine
} } University of Missouri
} } Columbia, MO 65211
} } Tel: (573) 882-8304
} } Fax: (573) 884-5414
} } Email: tindallr-at-missouri.edu
} } Web: http://www.biotech.missouri.edu/emc/
} }
} }
} }
} }
} }
} } -----Original Message-----
} } } From: Beavers, Roy [mailto:rbeavers-at-post.cis.smu.edu]
} } Sent: Wednesday, July 19, 2000 10:49 AM
} } To: 'Microscopy Listserver'; Microprobe List (E-mail)
} } Subject: Images of Zeolites
} }
} }
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } List,
} }
} } I have a customer from the chemistry department that wants to obtain
} some
} } SEM images of zeolite crystals in a flyash material (fine grain powder).
} My
} } question is how to prep this material to get it into the probe? Any
} ideas or
} } suggestions would be appreciated.
} }
} } Thanks
} }
} } Roy Beavers
} } Southern Methodist University
} } Dept. of Geological Sciences
} } Electron Microprobe Lab
} } P.O. Box 750395
} } Dallas, Tx 75275
} } voice: 214-768-2756
} } fax: 214-768-2701
} } E-mail: rbeavers-at-mail.smu.edu
} }
} }
} }
}
}
} Kent Hampton
}
}
} Department of Entomology
} khampton-at-oz.oznet.ksu.edu
} Phone: 785 532-4746
}



From daemon Fri Jul 21 08:19:07 2000



From: Nazlia Samodien :      ctssamodien-at-samiot.uct.ac.za
Date: Fri, 21 Jul 2000 08:03:00 -0500
Subject: Re: Coating of TEM viewing screens

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We purchased a new 15cm diam. screen a year ago from the Electron Optics
division of Phillips S.A for about R12000. They had quoted an amount of
around R13500 for having our phospherous screen resurfaced. This amount
probably included the shipping of the screen to and from the Phillips
agents. Obviously, from South Africa everything appears to escalate, the
further across the ocean we go, due to our exchange rates.
However, since then, an American based company (Sorry I don't have the
name) has taken over Phillips and Zeiss has been given the contract for the
service of the equipment.
Just something to consider before you make your decision.
Nazlia


_____________________________________________________

Nazlia Samodien

Cardiovascular Research Unit
Dept. of Cardiothoracic Surgery
University of Cape Town Medical School
Anzio Rd, Observatory, 7925, South Africa

Tel. +27 21 406 6398 (office)
+27 21 406 6476 (Secretary)
Fax + 27 21 448 5935


Email: ctssamodien-at-samiot.uct.ac.za





From daemon Fri Jul 21 08:23:46 2000



From: William H Roberts :      William.H.Roberts-at-usa.dupont.com
Date: Fri, 21 Jul 2000 08:11:02 -0500
Subject: Plasma Etcher Repair

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I have a March Plasmod (Plasma Etcher) which no longer produces the rf
needed to generate the plasma. Does anyone have a surplus/broken/obsolete
unit that could possibly still have operable electronics that they want to
get rid of? I'd like to try to repair mine before declaring it junk. (if
it can't be repaired economically, perhaps someone will need the parts on
it that are still good.)





From daemon Fri Jul 21 08:26:43 2000



From: ay813-at-chebucto.ns.ca ()
Date: Fri, 21 Jul 2000 08:14:13 -0500
Subject: books on LM

Contents Retrieved from Microscopy Listserver Archives
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Email: ay813-at-chebucto.ns.ca
Name: Robert Harnum

Question: 1. Which books do you recommend that a person new to light
microscopes read regarding the use and care of light microscopes? I cannot
find very much about the care of light microscopes. I want to look at
bacteria.

---------------------------------------------------------------------------





From daemon Fri Jul 21 09:11:03 2000



From: Nazlia Samodien :      ctssamodien-at-samiot.uct.ac.za
Date: Fri, 21 Jul 2000 15:51:18 +0200
Subject: Coating of TEM viewing screens

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We purchased a new 15cm diam. screen a year ago from the Electron Optics
division of Phillips S.A for about R12000. They had quoted an amount of
around R13500 for having our phospherous screen resurfaced. This amount
probably included the shipping of the screen to and from the Phillips
agents. Obviously, from South Africa everything appears to escalate, the
further across the ocean we go, due to our exchange rates.
However, since then, an American based company (Sorry I don't have the
name) has taken over Phillips and Zeiss has been given the contract for the
service of the equipment.
Just something to consider before you make your decision.
Nazlia


_____________________________________________________

Nazlia Samodien

Cardiovascular Research Unit
Dept. of Cardiothoracic Surgery
University of Cape Town Medical School
Anzio Rd, Observatory, 7925, South Africa

Tel. +27 21 406 6398 (office)
+27 21 406 6476 (Secretary)
Fax + 27 21 448 5935


Email: ctssamodien-at-samiot.uct.ac.za











From daemon Fri Jul 21 09:51:14 2000



From: michael shaffer :      mshaf-at-darkwing.uoregon.edu
Date: Fri, 21 Jul 2000 07:47:26 -0700
Subject: RE: SEM/EDX Mapping - What is accuracy and why does area% not equal 100?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Date sent: Fri, 21 Jul 2000 08:14:13 -0500
To: Microscopy-at-sparc5.microscopy.com
} From: ay813-at-chebucto.ns.ca ()



Tami writes ...

} ...
} ... I am trying to use this data to look at
} the differences between uranium precipitates
} ...
} ... However, there is a huge difference between the sum
} of all of the area%s reported for a given sample
} (which includes a "no element" area%). The total summed
} area % for these sample/scans ranges from 20% to 87%.
} ...
} Can anyone explain to me what it means when the total area%
} of all of the possible associations mapped sums to
} much less than 100%?
} What are the
}

The results of this type of spacial elemental analysis should have
resulted in two other calculated percentages ... (1) undefined pixels
and (2) multiply defined pixels. Depending on the software, the first
is generally included in the total you report, but not the second. It
is even possible neither have been included in the total. Do you have
access to these numbers, or the pixel maps???

cheerios, =shAf= :o)

{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {}
Michael Shaffer, R.A. - mshaf-at-darkwing.uoregon.edu
Geological Science's Electron Probe Facility - University of Oregon
http://epmalab.uoregon.edu/





From daemon Fri Jul 21 09:59:41 2000



From: michael shaffer :      mshaf-at-darkwing.uoregon.edu
Date: Fri, 21 Jul 2000 08:05:50 -0700
Subject: RE: Film Scanners

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The following tip was passed to me by a user in the Scottish
Agricultural College for spreading a monolayer of starch granules:

Dip a polished acrylic plastic rod into the powder and roll it gently
on the surface of a double-sided carbon tab. The method has two
advantages - grains are electrostatically attracted to the surface of
the rod forming a monolayer, and the rod applies enough pressure
to ensure that the particles are stuck down across a broad base,
improving continuity of the metal coating on the grain with that of
the tab surface and stub, minimizing charging.

It works for other particulates too, but can only be recommended
for hard particulates that will withstand light compressive forces
without damage.

Chris

} From: "Huggins, Bradley J" {HUGGINBJ-at-bp.com}
To: "Tindall, Randy D." {TindallR-at-missouri.edu} ,
"'Kent Hampton'"
{khampton-at-oz.oznet.ksu.edu}
Copies to: "'microscopy-at-sparc5.microscopy.com'"
{microscopy-at-sparc5.microscopy.com}



richard writes ...

} ...
} I am still looking for a film scanner for 4X5 film.
} ...

Flatbed scanners will not adapt very well to the optical density of
films. At best, they may not achieve 3.0. If yours are TEM films
then you probably want a scanner which is capable of near OD=4. The
only scanner I'm aware of, rated at OD=3.8, also reasonably priced is
the Polaroid 4x5 Ultra (~$4500). I realize this is much more
expensive than the scanners you are asking about, although they should
be adequate for SEM films.

cheerios, =shAf= :o)

{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {}
Michael Shaffer, R.A. - mshaf-at-darkwing.uoregon.edu
Geological Science's Electron Probe Facility - University of Oregon
http://epmalab.uoregon.edu/





From daemon Fri Jul 21 10:30:07 2000



From: prenitzer,brenda s :      prenitzer-at-lucent.com
Date: Fri, 21 Jul 2000 11:20:31 -0400
Subject: Conductive Epoxy?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Greetings!

Does anyone have any recommendations for a conductive epoxy (other the Ag
paint) that can be used to mount specimens to a stud that will be used for
both polishing and subsequent analysis? (i.e., mechanically durable, water
resistant and stable under ion bombardment)

Any info would be appreciated.

Brenda I. Prenitzer, Ph.D.
Member of Technical Staff
Cirent Semiconductor (Lucent Technologies)
9333 S. John Young Parkway
6D-Lab
Orlando, FL 32819-8612

Phone: 407 371 7108
Fax: 407 371 6999

prenitzer-at-lucent.com
bsp101-at-worldnet.att.net




From daemon Fri Jul 21 10:42:48 2000



From: MICHAEL DELANNOY :      delannoy-at-mail.jhmi.edu
Date: Fri, 21 Jul 2000 11:19:07 -0400 (EDT)
Subject: Confocal sale

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



For Sale:
Noran Oz Confocal Laser Scanning Microscope System with a new
Krypton-Argon laser, red HeNe, SGI O2 (acquisition), SGI Indy R5000
(analysis) and an Olympus IX 50 inverted microscope with 20x, 60x and 100x
Plan Apo lens'. This system is only 4 years old and fully operational.
Some highlighted features are:

AOD laser scanning, allows for very fast acquisition
rates (up to 480 frames/sec), for live localization, and
slow scan for fixed material.

Triple label capability (488, 568 and 633nm excitation)
with broad and narrow band emission filter sets.

Two and Three dimensional analysis software by Intervision,
Tiff and RGB formats (amoung others).

Deconvolution software allows for thinnest possible z-sectioning
for light microscopy.

Primary dichroic filter wheel upgrade for precise fluorophore
registration.

We are offering this system for $180,000, buyer to pay shipping.
I will personally give free on-site setup and training. Please contact
Michael Delannoy for further information (410) 955-1365.


Michael Delannoy
Assistant Director
JHMI Microscopy Facility
Johns Hopkins School of Medicine
Baltimore, Maryland




From daemon Fri Jul 21 10:52:23 2000



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Fri, 21 Jul 2000 08:43:19 -0700
Subject: Re: distinguishing polymer beads and "attached" microorganisms

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Winton,
With my experience of looking at organic materials, there is usually a
sulfur peak from the protein. If your specimen prep doesn't wash it away and
your sputter coat is thin, you might use the EDX to distinguish bacteria
from polymer. BTW, you should be able to look at both the polymer and
bacteria with no fixing or dehydrating, ad this should simplify identification.
At 11:20 AM 7/20/00 -0500, you wrote:

} Microscopists:
}
} A post-doc in our Chemical Engineering department came to me with
} this need - to quote:
}
} "Is there a way to distinguish between white polymer "buds" and
} microorganisms in a black-and-white SEM picture at a magnification of
} 8000X ? Both the cultured "bugs" and "buds" have the same size,
} shape, and appearance in/on spherical carbon/polymer beads that are
} sliced in half, fixed, mounted, and then viewed under the SEM".
}
} As you can see, it would appear that the polymer beads have surface
} textures/features ("buds") that are of comparable "shape" and "size"
} to the microorganisms cultured on the surface of the beads. I have
} yet to see the photos, so I can not comment on just what he is, or is
} not, seeing. The sample prep, as I understand it (never having done
} it!):
}
} 1. fixation in 1% glutaraldehyde with 0.1 molar cacodylate (excuse
} any misspellings)
} 2. post-fix of 1% osmium tetroxide in 0.1 molar cacodylate
} 3. dehydration in ethanol
} 4. critical point drying
} 5. sputter coating
}
} (whew!.....a lot more complicated than powder on a carbon sticky tab!!)
}
} So, based on this information, can any of you suggest a/other
} (pre-SEM) processing technique(s) (stains?) that he and our
} microscopist (in the Biology department) might utilize to help
} "differentiate" these "buds" from the cultured microorganisms?
}
} Winton
}
} PS: you can direct your feedback to me or the listserv - in either
} case I will compile the responses and have them available should any
} of you out there have need for them
}
} PS #2: I have an SEM too, with a Be-windowed EDX detector (= no
} light-element detection)
}
}
} --
}
} Winton C. Cornell, Ph.D.

Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca




From daemon Fri Jul 21 10:54:32 2000



From: Sinkler, Wharton :      WSinkler-at-uop.com
Date: Fri, 21 Jul 2000 10:45:48 -0500
Subject: RE: Film Scanners

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



We are also pricing scanners now. If anyone out there has experiences with
the Umax Powerlook 3000, particularly used to scan TEM negatives, I'd be
grateful for your insights.

Thanks,
Wharton Sinkler
UOP LLC
Des Plaines, IL

} -----Original Message-----
} From: michael shaffer [SMTP:mshaf-at-darkwing.uoregon.edu]
} Sent: Friday, July 21, 2000 10:06 AM
} To: richard black; Microscopy news group
} Subject: RE: Film Scanners
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} richard writes ...
}
} } ...
} } I am still looking for a film scanner for 4X5 film.
} } ...
}
} Flatbed scanners will not adapt very well to the optical density of
} films. At best, they may not achieve 3.0. If yours are TEM films
} then you probably want a scanner which is capable of near OD=4. The
} only scanner I'm aware of, rated at OD=3.8, also reasonably priced is
} the Polaroid 4x5 Ultra (~$4500). I realize this is much more
} expensive than the scanners you are asking about, although they should
} be adequate for SEM films.
}
} cheerios, =shAf= :o)
}
} {} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {}
} Michael Shaffer, R.A. - mshaf-at-darkwing.uoregon.edu
} Geological Science's Electron Probe Facility - University of Oregon
} http://epmalab.uoregon.edu/
}
}



From daemon Fri Jul 21 11:19:28 2000



From: BNguyen260-at-aol.com
Date: Fri, 21 Jul 2000 12:10:30 EDT
Subject: Re: Conductive Epoxy?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Brenda,
We, Electron Mictroscopy Sciences, did offering this TWO PART CONDUCTIVE
SILVER PAINT, product number 12642-14. Please contact us at 1-800-523-5874.
Meatime, we believe some other EM supply houses also carrying this product.
Regards,
Bang Nguyen



From daemon Fri Jul 21 11:36:48 2000



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Fri, 21 Jul 2000 11:22:53 -0500
Subject: Re: SEM/EDX Mapping - What is accuracy and why does area% not

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I would strongly guess that the answer will lie with how the area fractions
are determined as Michael Sshaffer (shAf) has suggested. I am not familiar
with the Noran system so I can only offer general comments.

Depending on the collection conditions, it is possible that you have a lot
of pixels with no x-ray counts, or counts that are indistinguishable from
background. What are you conditions and counts per pixel?

I would certainly think that with a proper map that you would approach 100%
coverage. Perhaps you can put some images up on a web site so those of us
interested might take a look at them. Or you might e-mail a copy _just to
those of us interested_. Do not send a copy to the whole list!

Warren S.

At 12:33 PM 7/20/2000 -0600, you wrote:
} I am a student with a great deal of SEM-EDX mapping data that was obtained
} on an Amray 1830 SEM with a Noran Pioneer Series II x-ray analyzer with
} micro-Z detector. I am trying to use this data to look at the differences
} between uranium precipitates obtained in a batch study with apatite
} (Ca5(PO4)3(F,OH)) and potassium. I'm getting interesting trends based on
} the area% association of U-Ca-K-P and pH. However, there is a huge
} difference between the sum of all of the area%s reported for a given sample
} (which includes a "no element" area%). The total summed area % for these
} sample/scans ranges from 20% to 87%. The elemental analyses of these
} precipitates indicated that most (97 to 99.9 wt%) of any given sample was
} made up of U, K, Ca, and P.
} Can anyone explain to me what it means when the total area% of all of the
} possible associations mapped sums to much less than 100%? What are the
} possible differences between samples or scans that sum to near 100% and
} those that sum to 20%?
} Tami Thomas - tthomas-at-ch2m.com -

----------------------
Warren E. Straszheim
Materials Analysis and Research Lab
Iowa State University
23 Town Engineering
Ames IA, 50011-3232

Ph: 515-294-8187
FAX: 515-294-4563

E-Mail: wesaia-at-iastate.edu
Web: www.marl.iastate.edu

Scanning electron microscopy, x-ray analysis, and image analysis of materials
Computer applications and networking



From daemon Fri Jul 21 11:41:18 2000



From: Nathan Haese :      nathan_haese-at-CompuServe.COM
Date: Fri, 21 Jul 2000 12:31:57 -0400
Subject: Conductive Epoxy?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Brenda,

Try Epoxy Technology, Inc. (14 Fortune Crive, Billerica, MA 01821).

I purchased silver-filled epoxy from them for mounting ultrasonic
transducers. It worked well. I am not sure about microscopy application.
Ask them.

Good luck,

Nathan Haese
Lafayette, CA



From daemon Fri Jul 21 12:56:08 2000



From: default :      byron.johnson-at-medtronic.com
Date: Fri, 21 Jul 2000 12:46:21 -0500
Subject: image database software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am tasked with setting up an electronic library of SEM images, and I'm
looking for good database software applications for cataloging and
viewing images. Multi-user access and strong software technical support
are primary requirements. What are my options?




From daemon Fri Jul 21 13:40:49 2000



From: Geoff McAuliffe :      mcauliff-at-UMDNJ.EDU
Date: Fri, 21 Jul 2000 14:31:50 -0400
Subject: Re: Conductive Epoxy?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


"prenitzer,brenda s" wrote:

} Greetings!
}
} Does anyone have any recommendations for a conductive epoxy (other the Ag
} paint) that can be used to mount specimens to a stud that will be used for
} both polishing and subsequent analysis? (i.e., mechanically durable, water
} resistant and stable under ion bombardment)
}
} Any info would be appreciated.
}
} Brenda I. Prenitzer, Ph.D.
} Member of Technical Staff
} Cirent Semiconductor (Lucent Technologies)
} 9333 S. John Young Parkway
} 6D-Lab
} Orlando, FL 32819-8612
}
} Phone: 407 371 7108
} Fax: 407 371 6999
}
} prenitzer-at-lucent.com
} bsp101-at-worldnet.att.net

You might contact Ablestick Adhesive in Gardena, CA. I worked for them on summer
many (and I do mean many) years ago, they made silver epoxy and copper epoxy
that might fill the bill.

Geoff
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************





From daemon Fri Jul 21 14:46:12 2000



From: Winton Cornell :      winton-cornell-at-utulsa.edu
Date: Fri, 21 Jul 2000 14:31:04 -0500
Subject: nuclear track etching

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Microscopists:

Sorry to be fielding another question so soon after my "bugs and
polymer beads" question; this time, however, I am on a very different
track.

In a paper on nanowire arrays (nano-scale nickel wires) I note a
technique entitled "nuclear track etching" by which porous templates
were constructed in single-crystal muscovites. Apparently a
collimated alpha-particle beam was used to generate the porous
templates. (in which the Ni was later electrochemically deposited)

Do any of you know what sort of instrumentation this requires? We
would like to produce sub-micron-scale holes in polymers for
specialized battery development, and right now we're searching for a
technique to do this. (nuclear etching?)

Winton

PS: the paper on nanowires does not mention the instrumentation

.respond directly to me or to the listserv


--

Winton C. Cornell, Ph.D.
Department of Geosciences
The University of Tulsa
Tulsa, OK 74104
phone: 918-631-3248
fax: 918-631-2091

e-mail: winton-cornell-at-utulsa.edu




From daemon Fri Jul 21 15:55:28 2000



From: Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 21 Jul 2000 13:44:11 -0700
Subject: RE: Film Scanners

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 08:45 AM 7/21/00, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Try the Powerlook III. It does a nice job with chromes and negs.
Since the TEM neg does not have a color cast, best scanning is
by setting the scanner to transparent positive and reverse the
image in Photoshop.

Depending on what you intend to do with the image file, this
scanner should do it all. They run about $1100 with SCSI interface
or a bit more with USB.

gary g.


~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Modern surfers use PC boards. You can too at
http://photoweb.net
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~




From daemon Fri Jul 21 16:05:45 2000



From: Walck, Scott D. :      walck-at-ppg.com
Date: Fri, 21 Jul 2000 16:54:45 -0400
Subject: RE: Conductive Epoxy?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The silver epoxy that we use for the small angle cleavage technique and for
mounting samples on SEM stubs is H-22 epoxy from Epoxy Technology in
Billerica, Mass. This epoxy is viscous, has a long working time, and needs
to be heat cured.


-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)



} -----Original Message-----
} From: prenitzer,brenda s [mailto:prenitzer-at-lucent.com]
} Sent: Friday, July 21, 2000 11:21 AM
} To: 'List Server'
} Subject: Conductive Epoxy?
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListse} rver/FAQ.html
}
}
} --------------------------------------------------------------
} ---------.
}
}
}
} Greetings!
}
} Does anyone have any recommendations for a conductive epoxy
} (other the Ag
} paint) that can be used to mount specimens to a stud that
} will be used for
} both polishing and subsequent analysis? (i.e., mechanically
} durable, water
} resistant and stable under ion bombardment)
}
} Any info would be appreciated.
}
} Brenda I. Prenitzer, Ph.D.
} Member of Technical Staff
} Cirent Semiconductor (Lucent Technologies)
} 9333 S. John Young Parkway
} 6D-Lab
} Orlando, FL 32819-8612
}
} Phone: 407 371 7108
} Fax: 407 371 6999
}
} prenitzer-at-lucent.com
} bsp101-at-worldnet.att.net
}
}



From daemon Fri Jul 21 16:17:33 2000



From: Walck, Scott D. :      walck-at-ppg.com
Date: Fri, 21 Jul 2000 17:06:24 -0400
Subject: RE: image database software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Try Thumbsplus at www.cerious.com
This is a great program. It is shareware and relatively inexpensive.


-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)



} -----Original Message-----
} From: default [mailto:byron.johnson-at-medtronic.com]
} Sent: Friday, July 21, 2000 1:46 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: image database software
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListse} rver/FAQ.html
}
}
} --------------------------------------------------------------
} ---------.
}
}
} I am tasked with setting up an electronic library of SEM
} images, and I'm
} looking for good database software applications for cataloging and
} viewing images. Multi-user access and strong software
} technical support
} are primary requirements. What are my options?
}
}



From daemon Fri Jul 21 17:10:45 2000



From: Zuo :      jianzuo-at-ux1.cso.uiuc.edu
Date: Fri, 21 Jul 2000 16:58:51 -0500
Subject: Post-doc position in UHV TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi :

I have a open post-doc position and I am looking for a "hands-on"
microscopist who can do excellent science. The position is in the materials
science department, university of illinois, urbana-Champaign. I'd appreciate
your help in passing this along to potential applicants.

Here is the job ad:
****************************************************************************

Postdoctoral Research Associate in In-Situ Thin Film Growth and Electron
Microscopy

Applications are invited for a postdoctoral associate position at the Dept.
of Materials Science and Engineering of University of Illinois at Urbana and
Champaign to begin as soon as possible. The position will involve in-situ
thin film growth in a UHV transmission electron microscope and study of
interface structures using advanced electron microscopy at the
Frederick-Seitz Materials Research Laboratory. A vita and three letters of
recommendation should be sent to Prof. J.M. Zuo, Dept. of Materials Science
and Engineering, University of Illinois at Urbana and Champaign, 1304 W
Green Street, Urbana, IL 61801; 217-333-2736 (fax); (217) 244-6504 (phone);
jianzuo-at-uiuc.edu (email). The successful candidate should have experience
with UHV instruments and thin-film deposition. Experience with RHEED and
transmission electron microscopy is strongly desired. A Ph. D. in Physics
or Materials Science is required. The appointment is initially for one year
and may be renewed for second year.


To be advertised in Physics today
****************************************************************************

Sincerely

JM Zuo






From daemon Fri Jul 21 17:18:41 2000



From: wft03-at-health.state.ny.us
Date: Fri, 21 Jul 2000 18:07:31 -0400
Subject: Re: nuclear track etching

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html





Sorry to be fielding another question so soon after my "bugs and
polymer beads" question; this time, however, I am on a very different
track.

In a paper on nanowire arrays (nano-scale nickel wires) I note a
technique entitled "nuclear track etching" by which porous templates
were constructed in single-crystal muscovites. Apparently a
collimated alpha-particle beam was used to generate the porous
templates. (in which the Ni was later electrochemically deposited)

Do any of you know what sort of instrumentation this requires? We
would like to produce sub-micron-scale holes in polymers for
specialized battery development, and right now we're searching for a
technique to do this. (nuclear etching?)

Winton

PS: the paper on nanowires does not mention the instrumentation

.respond directly to me or to the listserv
Dear Winton,
In order to etch tracks with alpha particles, you need to give them
kinetic energies of at least a few MeV--nuclides which undergo alpha decay
typically produce ~5 MeV alphas. Thus, either a suitable radionuclide
source
or a cyclotron would be required. However, even with this kind of source
there may be problems. The text for my radiation sciences course, Nuclear
and Radiochemistry, by G. Friedlander, et al., has a section on nuclear
track
detectors in which it is stated, "For each material there is a critical
value of
specific ionization, (dE/dx)c, below which tracks are not registered. For
example, (dE/dx)c is ~ 13 MeV mg^-1 cm^2 for mica and ~4 MeV mg^-1
cm^2 for Lexan, two of the most widely used detectors. As a result mica
does not register ions with A {~30, Lexan is insensitive to ions with
A {~12."
Alphas have A = 4. Now this text is old (1981) and not too good, so the
technique for producing appropriate tracks may have been improved or
Friedlander may be incorrect for the kind of tracks you need. Also, the
tracks are produced when the incident particles ionize and/or displace
atoms
in the target, so the tracks may not be as small as you would like. Bear
in
mind that electrons incident on a specimen generate x-rays throughout a
teardrop-shaped volume about 1 micron across. Although alphas do not
have as great a spread as electrons, much of the ionization produced is
from secondary particles, and these will be electrons. If the paper you
refer
to tells you how to generate suitable tracks given a source of alphas, then
you are probably OK. Good luck.
Yours,
Bill Tivol





From daemon Fri Jul 21 21:32:51 2000



From: Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 21 Jul 2000 19:21:32 -0700
Subject: Re: image database software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 10:46 AM 7/21/00, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Consider ImageAXS. It is set up to manage your image database
and also to allow you to set up CDs with thumbnails and full
resolution files.

The other option is ThumbsPlus--but Cerious Software is rather
dysfunctional based on my experience with them.

Other options are Canto Version 5 Cumulus, Kudo Image Browser
{http://www.imspace.com} ,
Extensis/CreativePro Portfolio {http://creativepro.com} . See
{http://riecks.com/redbarns.html} or {http://riecks.com/crash/} . I believe
the Kudo software is still $49 for the web
download version. You can download a demo version for free and give it a spin.
Then there is Gallerymaker (www.rjhsoftware.com) which produces thumbnails
and webpages, all of which are customizable. Cheap as I remember too. I just
use it to batch process thumbs from whole directories.

There are other options too.

gg





From daemon Sat Jul 22 03:47:16 2000



From: Chris Jeffree :      cjeffree-at-srv0.bio.ed.ac.uk
Date: Sat, 22 Jul 2000 09:31:12 +0100
Subject: Re: nuclear track etching

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


*snip*
Winton
A brand of commercially-available polycarbonate filters is made
using this method. Nuclepore have pores mostly with perfectly
circular cross sections sizes from about 50nm to 12µm.
I am writing from home so unable to check, but I think they are
marketed by Whatman. Your usual supplier of filter media should be
able to source.

Chris

} We
} would like to produce sub-micron-scale holes in polymers for
} specialized battery development, and right now we're searching for a
} technique to do this. (nuclear etching?)
}
} Winton
}
} PS: the paper on nanowires does not mention the instrumentation
}
} .respond directly to me or to the listserv
} Dear Winton,
} In order to etch tracks with alpha particles, you need to give them
} kinetic energies of at least a few MeV--nuclides which undergo alpha decay
} typically produce ~5 MeV alphas. Thus, either a suitable radionuclide
} source
} or a cyclotron would be required. However, even with this kind of source
} there may be problems. The text for my radiation sciences course, Nuclear
} and Radiochemistry, by G. Friedlander, et al., has a section on nuclear
} track
} detectors in which it is stated, "For each material there is a critical
} value of
} specific ionization, (dE/dx)c, below which tracks are not registered. For
} example, (dE/dx)c is ~ 13 MeV mg^-1 cm^2 for mica and ~4 MeV mg^-1
} cm^2 for Lexan, two of the most widely used detectors. As a result mica
} does not register ions with A {~30, Lexan is insensitive to ions with
} A {~12."
} Alphas have A = 4. Now this text is old (1981) and not too good, so the
} technique for producing appropriate tracks may have been improved or
} Friedlander may be incorrect for the kind of tracks you need. Also, the
} tracks are produced when the incident particles ionize and/or displace
} atoms
} in the target, so the tracks may not be as small as you would like. Bear
} in
} mind that electrons incident on a specimen generate x-rays throughout a
} teardrop-shaped volume about 1 micron across. Although alphas do not
} have as great a spread as electrons, much of the ionization produced is
} from secondary particles, and these will be electrons. If the paper you
} refer
} to tells you how to generate suitable tracks given a source of alphas, then
} you are probably OK. Good luck.
} Yours,
} Bill Tivol
}
}
}


~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Dr Chris Jeffree
University of Edinburgh
Biological Sciences EM Facility
Daniel Rutherford Building
King's Buildings EDINBURGH EH9 3JH
Tel: +44 (0) 131 650 5345
FAX: +44 (0) 131 650 6563

Inveresk Cottage, 26 Carberry Road,
Inveresk, Musselburgh, Midlothian EH21 8PR, UK
Tel: +44 (0) 131 665 6062 / Mobile 07710 585 401
FAX: +44 (0) 131 653 6248
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~



From daemon Sat Jul 22 05:29:37 2000



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Sat, 22 Jul 2000 06:16:45 -0500
Subject: Conductive metallographic mounting medium

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Brenda I. Prenitzer wrote:
================================================================
Does anyone have any recommendations for a conductive epoxy (other the Ag
paint) that can be used to mount specimens to a stud that will be used for
both polishing and subsequent analysis? (i.e., mechanically durable, water
resistant and stable under ion bombardment)
=================================================================
The softness of silver generally makes it less than desired as a conductive
filler in this kind of application because of silver smearing, although
silver filled epoxies are available from SPI and others such as described on
URL
http://www.2spi.com/catalog/spec_prep/cond_adhes2.html

Can you consider using a conductive copper-filled diallyl phthalate such as
is available from both SPI and others. This is a thermoset resin system (it
requires a mounting press) and you can get an excellent metallographically
polished surface. See URL
http://www.2spi.com/catalog/spec_prep/metall2a.html#12 It is
certainly mechanically durable, water resistant and so far as it is possible
for any polymer, it is also stable under ion bombardment.

You would then literally glue the polished mount to the SEM mount of your
choice, which you would then prepare using your standard procedures.

This "solution" permits you to have the best of both worlds, first, a
mounting medium that was specifically designed for this kind of application
plus a final SEM mount ready to put into your SEM.

Disclaimer: SPI offers both silver filled epoxies as well as conductive
metallographic mounting media. But similar materials are available from
some of the other major firms supplying the microscopy and microanalysis
market as well.

Chuck
============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.spi.cc
########################
============================================





From daemon Sat Jul 22 07:16:22 2000



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Sat, 22 Jul 2000 08:03:00 -0500
Subject: Plasmod power electronics

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

William H. Roberts wrote:
======================================================
I have a March Plasmod (Plasma Etcher) which no longer produces the rf
needed to generate the plasma. Does anyone have a surplus/broken/obsolete
unit that could possibly still have operable electronics that they want to
get rid of? I'd like to try to repair mine before declaring it junk. (if
it can't be repaired economically, perhaps someone will need the parts on it
that are still good.)
======================================================

If what you are asking about is the pair of power tubes, then new ones can
be purchased from SPI Supplies, our part number 11022-AC for the pair of
power tubes. Details can be found on the SPI website given below. This was
a well built unit and there is not reason why it can't continue to give you
good service for years into the future.

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.spi.cc
########################
============================================





From daemon Sat Jul 22 07:51:10 2000



From: Mark Sanders :      msanders-at-biosci.cbs.umn.edu
Date: Sat, 22 Jul 2000 07:41:08 -0500
Subject: Re: image database software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} I am tasked with setting up an electronic library of SEM images, and I'm
} looking for good database software applications for cataloging and
} viewing images. Multi-user access and strong software technical support
} are primary requirements. What are my options?

Another very powerful database management option is Ugather®.
UGather® is a free multimedia database manager that can catalog images,
QuickTime movies, and audio files and store information related to each
file. The databases can be imported easliy into UPresent®. UPresent® is a
free multimedia presentation application designed for flexible, interactive
control of presentations. It is available for Windows and Mac OS.
You can download it from http://upresent.umn.edu/.
I have no commercial interests, just a satisfied user.
Cheers,
Mark
****************************************************
Mark A. Sanders University of Minnesota
Program Director Twin Cities Campus
Imaging Center St. Paul, MN 55108
23-35 Snyder Hall ph: 612-624-3454
1475 Gortner Ave. fax: 612-624-1799
http://biosci.umn.edu/imagingcenter





From daemon Sat Jul 22 11:12:09 2000



From: Edward Hirsch :      edhirsch-at-att.net
Date: Sat, 22 Jul 2000 11:58:20 -0500
Subject: image database software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Byron,

Allied High Tech has just introduced an image acquisition, measurement,
DATABASE, archiving and report generation software package (Micro-Vision and
Micro-Vision Explorer). The software is modular so the database portion
(Micro-Vision Explorer) can be purchased separately.

Micro-Vision Explorer has thumbnails for each image and search features for
the data you enter into the customizable fields. All fields are set up by
an administrator to meet your needs. Unique fields can be created for text
input or numeric input. The fields can have drop down menus if you choose.
Each field can have specific attributes such as: flagged for required input,
read only, archive, clear last input plus more. The search features
include: file name, date, specific field search, text contained any where in
the properties, fuzzy searches plus much more. It supports an unlimited
number of images. It has built in archiving capability with volume control.
Micro-Vision Explorer has an easy to use Windows interface and will run on
Windows 95, 98, NT and 2000 computers. All of the popular image formats are
supported. The software can be networked and password protection can be
implemented.

In addition to images, Micro-Vision Explorer is also capable of indexing,
searching and achieving plain text, Lotus AMI Pro, HTML, Microsoft Word,
Excel, Works, RTF, PowerPoint 97, Adobe Acrobat PDF, Word Perfect versions
5.0 thru 7, and Word Star versions 4 to 6 and 2000.

If you require more information please let me know off-line.

I work for Allied High Tech and have a financial interest in this and all
products Allied High Tech sells.

Sincerely,

Ed Hirsch



*************************************************
Edward A. Hirsch
Product Application Specialist
Allied High Tech Products
2376 East Pacifica Place
Rancho Dominguez, CA 90220
ph: (919) 846-9628
vm:(800)675-1118 x245
fx: (310)762-6808
http://www.alliedhightech.com

Equipment and Consumables for Metallurgical Sample Preparation
*************************************************

-----Original Message-----
} From: default [mailto:byron.johnson-at-medtronic.com]
Sent: Friday, July 21, 2000 12:46 PM
To: Microscopy-at-sparc5.microscopy.com


I am tasked with setting up an electronic library of SEM images, and I'm
looking for good database software applications for cataloging and
viewing images. Multi-user access and strong software technical support
are primary requirements. What are my options?





From daemon Sun Jul 23 12:13:32 2000



From: Rick Savoy :      rjsavoy-at-ibm.net
Date: Sun, 23 Jul 2000 09:51:13 -0700
Subject: Analytical Engineer Position Available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Job description for analytical engineer, Defect and Thin Film
Characterization Laboratory (DTCL), Applied Materials, Inc. Santa Clara, CA

Job Summary
Active participation in product division contamination control and defect
reduction programs. Prioritized by value to the product divisions, conduct
internal and external defect characterization focused on defect root cause
analysis. Interact with external experts and DTCL members to leverage value
to product divisions. Document and communicate root cause solutions and
strategic information to internal customers and strategic personnel in
product divisions and functions.

Minimum Requirements:
MS in Material Science/ Physics with 3 years hands-on experience in
analytical instrumentation applied for defect/ thin film analysis. Must
demonstrate:
(1) Good hands-on capabilities as well as sound theoretical understanding of
SEM, EDX, WDX, and FIB applications for particle defect/ thin film analysis.
In addition, practical experience with Auger is a plus.
(2) Experience in wafer surface defect detection using light scattering
technique, e.g. Applied Materials WF736, Excite, Tencor 62XX and SP1-TBI.
(3) Experience in TEM sample preparation and bulk samples cross-sectioning.
(4) Ability to work independently with minimum of supervision. Must have
good technical documentation/computer skills and is a team player.


Please send resume via e-mail to:
richard_savoy-at-amat.com




From daemon Sun Jul 23 12:49:16 2000



From: Djemge-at-aol.com
Date: Sun, 23 Jul 2000 13:33:47 EDT
Subject: TEM Biological Transmission Electron Employment

Contents Retrieved from Microscopy Listserver Archives
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I am seeking a Transmission Electron Micoscopy Position in the Chicago, IL or
Los Angeles, CA area. I have 34 years of experience as a Biological
Transmission Electron Microscopist in research and clinical areas, have held
MSA certification for 21 years, and have a Bachelors Degree. If you know of
positions in the above areas or know of resources for job postings in
Electron Microscopy, please contact me via the following:

Elaine R. Sundin
6033 N. Sheridan Rd. #38L
Chicago, IL 60660
Phone 773-728-8083
e-mail djemge-at-aol.com



From daemon Mon Jul 24 10:32:54 2000



From: Tim E. Harper :      tim-at-cmp-cientifica.com
Date: Mon, 24 Jul 2000 17:18:56 +0200
Subject: Announcement: TNT (Trends In Nanotechnology) 2000

Contents Retrieved from Microscopy Listserver Archives
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Dear Colleagues,

TNT (Trends In Nanotechnology) 2000 will take place this year in the UNESCO
World heritage city of Toledo. The aim of this conference will be to focus
on the applications of Nanotechnology and to bring together in a Forum many
of the major European groups working in this field. www.cientifica.com
{http://www.cientifica.com}
TNT 2000 will be held in the Monastery 'San Pedro Mártir' in the historic
city of Toledo, Spain from the 16th - 20th October 2000.
The aim of this conference will be to focus on the applications of
Nanotechnology and to bring together in a Forum various groups working in
this field. In addition to the normal program of keynote speakers, oral
presentations and poster sessions, it is also planned to hold workshops on
topics such as "overcoming obstacles to industrial production".
The first announcement is available here as a MS Word or Acrobat pdf
document
In addition to the normal program of keynote speakers, oral presentations
and poster sessions, it is also planned to hold workshops on topics such as
"overcoming obstacles to industrial production". Currently 2 workgroups,
"Nanofabrication: printing" and "Nanofabrication: Self-Assembly" from the
5th MEL/ARI-NID program (European Commission) have decided to actively
participate at this conference. They will hold their NID (Nanotechnology
Information Devices) meeting during TNT2000.
Scientific Program
The scientific program will cover a wide range spectrum of Nanotechnology
research and related areas including keynote lectures, oral presentations,
posters and a product/instrument exhibition. A crucial issue during a
conference is time allowed to discussions between participants in order to
exchange ideas and therefore possibly define strategies in order to solve
real problems. During TNT'2000, following each session, large breaks will be
set-up in order to allow these discussions. This allows a high degree of
interactivity both between research groups, and between researchers and
exhibitors.
The TNT conference will allow either Academic institutions or Industrials
(such as IBM, Lucent, Philips, Thomson, Sony, etc.) to present their
research and foster cooperation between both.
Key topics
* Nanotubes
* Local Electromagnetic Phenomena
* Quantum + DNA Computing
* Devices and Machines
* Nanoscale Integration
* Nanobiology
* Nanotechnology for space Applications
* Molecular Nanotechnology
* Nanomedicine
* Nanostructure Behaviour Modelling
* Nanomaterials
* Nanofabrication Tools and Techniques
* Ultimate Limits of Measurement


More information about the Workshop (Keynote Speakers, Accommodation,
Committees, schedule, social events, etc.) is available at:
http://www.cientifica.com/ {http://www.cientifica.com/}

If you need more information, please do not hesitate to contact me.
Regards
Tim E. Harper


*****************************************************************
Tim E. Harper Managing Director
CMP Cientifica s.l.
Space & NanoTechnology Division
Phone +34 91 640 71 85 Fax +34 91 640 71 86
http://www.cmp-cientifica.com/ {http://www.cmp-cientifica.com/}




From daemon Mon Jul 24 10:48:22 2000



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Mon, 24 Jul 2000 08:35:40 -0700
Subject: Re: image database software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Byron,
You will find that the Quartz Imaging Corp. (www.quartzimaging.com) image
databasing software is very complete, easy to use and access and fully
supported. It has several options for intranet or web-based access and is
the fastest I've ever seen.
At 12:46 PM 7/21/00 -0500, you wrote:
}
} I am tasked with setting up an electronic library of SEM images, and I'm
} looking for good database software applications for cataloging and
} viewing images. Multi-user access and strong software technical support
} are primary requirements. What are my options?
}
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca




From daemon Mon Jul 24 10:54:34 2000



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Mon, 24 Jul 2000 08:46:09 -0700
Subject: Conductive Epoxy?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Brenda,
I use the Cu-filled Kulzer Technovit 5000 cold-curing resin to mount and
polish metal samples that I do not want to carbon-coat. I believe it is made
in Germany, but imported by Energy Beam Sciences.}
} Greetings!
}
} Does anyone have any recommendations for a conductive epoxy (other the Ag
} paint) that can be used to mount specimens to a stud that will be used for
} both polishing and subsequent analysis? (i.e., mechanically durable, water
} resistant and stable under ion bombardment)
}
} Any info would be appreciated.
}
} Brenda I. Prenitzer, Ph.D.

Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca




From daemon Mon Jul 24 11:41:24 2000



From: John J. Bozzola :      bozzola-at-siu.edu
Date: Mon, 24 Jul 2000 11:23:54 -0500
Subject: SEM:donated SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} A colleaque needs to know the value of an ISI Super IIIA that was
} dontated to their institution (probably for tax credit purposes to
} the donor).
}
} If someone is able to give a reasonable figure to this individual,
} please send the figure to the following address:
}
} DWARD-at-flemingc.on.ca
}
} Thank you on his behalf.
--
####################################################################
John J. Bozzola, Ph.D., Director
Micro-Imaging and Analysis Center
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
####################################################################



From daemon Mon Jul 24 15:24:52 2000



From: MICHAEL DELANNOY :      delannoy-at-mail.jhmi.edu
Date: Mon, 24 Jul 2000 15:58:37 -0400 (EDT)
Subject: Re: image database software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Gary,
Let me know where I can get an O2 for only $750, also where I can
get a confocal system (not pcm) for under 200K. The point is we are not
dumping the system, it is heavily used, check out
www.med.jhu.edu/micfac/reserve.cgi, for availability, we simply need a
Uv laser and rather than retro-fitting we decided to buy a new one. I
would put any of our deconvoluted confocal images up agaisnt any system
out there and feel confident that they are as good if not better.

Mike D.

On Fri, 21 Jul 2000, Gary Gaugler wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} At 10:46 AM 7/21/00, you wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } I am tasked with setting up an electronic library of SEM images, and I'm
} } looking for good database software applications for cataloging and
} } viewing images. Multi-user access and strong software technical support
} } are primary requirements. What are my options?
}
}
} Consider ImageAXS. It is set up to manage your image database
} and also to allow you to set up CDs with thumbnails and full
} resolution files.
}
} The other option is ThumbsPlus--but Cerious Software is rather
} dysfunctional based on my experience with them.
}
} Other options are Canto Version 5 Cumulus, Kudo Image Browser
} {http://www.imspace.com} ,
} Extensis/CreativePro Portfolio {http://creativepro.com} . See
} {http://riecks.com/redbarns.html} or {http://riecks.com/crash/} . I believe
} the Kudo software is still $49 for the web
} download version. You can download a demo version for free and give it a spin.
} Then there is Gallerymaker (www.rjhsoftware.com) which produces thumbnails
} and webpages, all of which are customizable. Cheap as I remember too. I just
} use it to batch process thumbs from whole directories.
}
} There are other options too.
}
} gg
}
}
}




From daemon Mon Jul 24 15:57:01 2000



From: :      bv-ltd-at-lycos.com
Date: Mon, 24 Jul 2000 15:51:12 -0500 (CDT)
Subject: BRAND NEW CARS FROM 23 POUNDS PW

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


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From daemon Mon Jul 24 18:27:18 2000



From: Klein,Roy :      RKLEIN-at-cmiinternational.com (by way of Nestor J.
Date: Mon, 24 Jul 2000 18:11:30 -0500
Subject: Position Open: Physicist R&D In Using X-ray Fluorescense For

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Colleagues....

I'm passing this along if anyone is interested. Reply to CMI..

Nestor

--------------------------------


Opportunity For Employment


We are CMI International, one of the leading manufacturers of Coating
Measurement Instruments in the world. Our products utilize x-ray, Beta
Backscatter, eddy current, inductance, conductance, and other leading
technologies. We believe that people want a career opportunity that allows
them to be creative, productive, and challenged. If you desire to work in
an environment that supports these qualities, we are currently seeking an
individual to join our Research/Engineering team.

Position Available: Physicist

Principle Involvements: X-ray Fluorescence Instrument Research and
Customer Applications For Coating and Composition Measurements.

Description of Potential Involvements: Spectral processing including use
of linear fitting, Gausian fitting, peak deconvolution as well as other
techniques. Fundamental parameter utilization and enhancements are also
included. Also there will be involvement with detectors, x-ray tubes,
optics, and electronics hardware to optimize the overall system
performance. State of the art techniques will be used in most areas of
involvement.

Requirements: Ph.D or M.S. in Physics. Prefer at least some specialization
in nuclear or x-ray area. Ability to use advanced mathematical techniques
for spectral processing and fundamental parameter utilization and
enhancements is desireable. Also capability to utilize Visual C++ to test
out and impliment ideas is very desireable.


If you are interested in pursuing a career with us please email your resume
to rklein-at-cmiinternational.com or fax your resume to R. Klein -at- (847)
439-4425. If you would like to discuss potential opportunities at the
Denver X-ray Conference please leave a note at the front desk of the
Denver Marriot Tech Center Hotel. Look us up at our web site which is:
cmiinternational.com.







From daemon Mon Jul 24 21:09:43 2000



From: Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 24 Jul 2000 18:58:04 -0700
Subject: Fwd: Re: Confocal sale

Contents Retrieved from Microscopy Listserver Archives
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} Date: Mon, 24 Jul 2000 17:04:44 -0700
} To: MICHAEL DELANNOY {delannoy-at-mail.jhmi.edu}
} From: Gary Gaugler {gary-at-gaugler.com}
} Subject: Re: Confocal sale
}
} I just sold an Indigo 2 Extreme complete system for $900. There
} are numerous O2 boxes around for between $600-$1200, depending
} on whether it is a 5K or 10K CPU and how much memory it has.
} The O2 is a nice toaster for sure. But it is rather dated.
}
} Any inverted scope is not going to have the image quality and
} resolution of a vertical unit. My worst experience was with an
} Olympus IMT-2. I dumped it for $6K and was glad to be rid of it.
}
} If your system is working OK for you, all the better. I was just
} trying to point out the impact of technology advancement on
} after-the-purchase value.
}
} gary g
}
}
} At 12:15 PM 7/24/00, you wrote:
}
} } Gary,
} } I don't know where you estimates come from, but let me know where
} } I can get an O2 for $750. Anyway we aren't dumping this system,
} } this system is still making money for our facility (check out our reservation
} } scheduler www.med.jhu.edu/micfac/reserve.cgi), the Noran Oz is always booked.
} } The reason for the sale is to get a confocal system with a Uv laser, we don't
} } want to retro fit this one, so it seemed simplier to sell it. I would put
} } any of our deconvoluted confocal images up against any other system
} } available,
} } and feel confident that the imaging is as good if not better.
} }
} }
} } On Fri, 21 Jul 2000, Gary Gaugler wrote:
} }
} } } How many Mercedes come with this? The O2 is worth about
} } } $750. The Indigo 5000 is not worth more than $750. And the
} } } inverted Olympus is barely worth a mention.
} } }
} } } No wonder you want to dump it.
} } }
} } } gg
} } }
} } }
} } }
} } } At 08:19 AM 7/21/00, you wrote:
} } } } ------------------------------------------------------------------------
} } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } } -----------------------------------------------------------------------.
} } } }
} } } }
} } } }
} } } } For Sale:
} } } } Noran Oz Confocal Laser Scanning Microscope System with a new
} } } } Krypton-Argon laser, red HeNe, SGI O2 (acquisition), SGI Indy R5000
} } } } (analysis) and an Olympus IX 50 inverted microscope with 20x, 60x and
} } 100x
} } } } Plan Apo lens'. This system is only 4 years old and fully operational.
} } } } Some highlighted features are:
} } } }
} } } } AOD laser scanning, allows for very fast acquisition
} } } } rates (up to 480 frames/sec), for live localization, and
} } } } slow scan for fixed material.
} } } }
} } } } Triple label capability (488, 568 and 633nm excitation)
} } } } with broad and narrow band emission filter sets.
} } } }
} } } } Two and Three dimensional analysis software by Intervision,
} } } } Tiff and RGB formats (amoung others).
} } } }
} } } } Deconvolution software allows for thinnest possible z-sectioning
} } } } for light microscopy.
} } } }
} } } } Primary dichroic filter wheel upgrade for precise fluorophore
} } } } registration.
} } } }
} } } } We are offering this system for $180,000, buyer to pay shipping.
} } } } I will personally give free on-site setup and training. Please contact
} } } } Michael Delannoy for further information (410) 955-1365.
} } } }
} } } }
} } } } Michael Delannoy
} } } } Assistant Director
} } } } JHMI Microscopy Facility
} } } } Johns Hopkins School of Medicine
} } } } Baltimore, Maryland
} } }




From daemon Mon Jul 24 21:09:44 2000



From: Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 24 Jul 2000 18:57:40 -0700
Subject: Fwd: Re: Confocal sale

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} Date: Mon, 24 Jul 2000 17:07:20 -0700
} To: MICHAEL DELANNOY {delannoy-at-mail.jhmi.edu}
} From: Gary Gaugler {gary-at-gaugler.com}
} Subject: Re: Confocal sale
}
} Check out this link for an almost complete O2/10K system.
} It is in the range I quoted.
}
} http://cgi.ebay.com/aw-cgi/eBayISAPI.dll?ViewItem&item=388534054
}
} gg
}
}
} At 12:15 PM 7/24/00, you wrote:
}
} } Gary,
} } I don't know where you estimates come from, but let me know where
} } I can get an O2 for $750. Anyway we aren't dumping this system,
} } this system is still making money for our facility (check out our reservation
} } scheduler www.med.jhu.edu/micfac/reserve.cgi), the Noran Oz is always booked.
} } The reason for the sale is to get a confocal system with a Uv laser, we don't
} } want to retro fit this one, so it seemed simplier to sell it. I would put
} } any of our deconvoluted confocal images up against any other system
} } available,
} } and feel confident that the imaging is as good if not better.
} }
} }
} } On Fri, 21 Jul 2000, Gary Gaugler wrote:
} }
} } } How many Mercedes come with this? The O2 is worth about
} } } $750. The Indigo 5000 is not worth more than $750. And the
} } } inverted Olympus is barely worth a mention.
} } }
} } } No wonder you want to dump it.
} } }
} } } gg
} } }
} } }
} } }
} } } At 08:19 AM 7/21/00, you wrote:
} } } } ------------------------------------------------------------------------
} } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } } -----------------------------------------------------------------------.
} } } }
} } } }
} } } }
} } } } For Sale:
} } } } Noran Oz Confocal Laser Scanning Microscope System with a new
} } } } Krypton-Argon laser, red HeNe, SGI O2 (acquisition), SGI Indy R5000
} } } } (analysis) and an Olympus IX 50 inverted microscope with 20x, 60x and
} } 100x
} } } } Plan Apo lens'. This system is only 4 years old and fully operational.
} } } } Some highlighted features are:
} } } }
} } } } AOD laser scanning, allows for very fast acquisition
} } } } rates (up to 480 frames/sec), for live localization, and
} } } } slow scan for fixed material.
} } } }
} } } } Triple label capability (488, 568 and 633nm excitation)
} } } } with broad and narrow band emission filter sets.
} } } }
} } } } Two and Three dimensional analysis software by Intervision,
} } } } Tiff and RGB formats (amoung others).
} } } }
} } } } Deconvolution software allows for thinnest possible z-sectioning
} } } } for light microscopy.
} } } }
} } } } Primary dichroic filter wheel upgrade for precise fluorophore
} } } } registration.
} } } }
} } } } We are offering this system for $180,000, buyer to pay shipping.
} } } } I will personally give free on-site setup and training. Please contact
} } } } Michael Delannoy for further information (410) 955-1365.
} } } }
} } } }
} } } } Michael Delannoy
} } } } Assistant Director
} } } } JHMI Microscopy Facility
} } } } Johns Hopkins School of Medicine
} } } } Baltimore, Maryland
} } }




From daemon Mon Jul 24 22:32:17 2000



From: Richard Easingwood :      richard.easingwood-at-stonebow.otago.ac.nz
Date: Tue, 25 Jul 2000 15:24:54 +1200
Subject: Nitrogen developer agitation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear All,
Just wondering if anyone out there uses a nitrogen bubble system to agitate
their film processing solutions in tanks. We are considering such a system
but I would like a clearer idea about the set-up before going ahead and
asking our workshop to make the system. At present we use Kodak 4489 film
and ordinary tanks with Kodak D19 developer and Ilford Hypam, this works
fine except occasionally when people forget to agitate the film.

If anyone has such a system which is being used for sheet negs I would
appreciate a rough description how it works. ie

1) what the layout of tubes on the bottom of the tanks is like

2) what kind of control system is used to set the bubbling intervals
(presumably something like a solenoid on a timer connected through a
regulator on to a N2-filled cylinder?)

Are there any problems or drawbacks to the systems which are in use? Are
they worth the trouble of setting up and maintaining?


Best regards,

Richard


Richard Easingwood
South Campus Electron Microscope Unit
School of Medical Sciences, University of Otago
PO Box 913, Dunedin
NEW ZEALAND

Telephone: 64-03-479 7301 Facsimile: 64-03-479 7254
mailto:richard.easingwood-at-stonebow.otago.ac.nz
http://anatomy.otago.ac.nz:800/Department/EMUnit.html
http://www.otago.ac.nz/anatomy/emunit/







From daemon Tue Jul 25 01:34:22 2000



From: Ron Doole :      ron.doole-at-materials.oxford.ac.uk
Date: Tue, 25 Jul 2000 09:00:51 +0100 (BST)
Subject: Re: Nitrogen developer agitation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Proscitech offers very pure, very fine silver powder in 20 g tubes. This can be
mixed with any suitable compound, which has several advantages.
I believe that using silver powder for mixing in with epoxy and similar
applications has several advantages. Obviously ProSciTech has an interest in
the use of this product.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

-----Original Message-----
} From: Mary Mager [SMTP:mager-at-interchange.ubc.ca]
Sent: Tuesday, July 25, 2000 1:46 AM
To: Microscopy-at-sparc5.microscopy.com


On Tue, 25 Jul 2000, Richard Easingwood wrote:

Hi Richard,

We have been using N2 burst in our developing tanks since long before I
came here over 20 years ago and I think that it is a great facility.
We are a large multi user facility that can be processing between 200 and
2000 sheets a week in two different processing lines. The only problem we
have is that occasionally users do not check that there is N2 in the gas
bottle before processing and hence it does not work. The importance of
correct agitation is obvious and lack of agitation is easily spotted when
a users has got it wrong (very rare).

We use systems bought from Kodak 30(ish) years ago. There are 6 tubes with
small holes in the bottom of our deep tanks, a solenoid valve controlled
by a timer and a N2 bottle with regulator. The timer can be set for off,
continous and timed (with variable burst times and off intervals). We
generally use 1 second burst every 10 seconds. The gas pressure is
adjusted to ensure we have the minimum flow with a good agitation
(around 10 psi).

An added advantage is that I can reload the films during the developing
time instead of hanging around, occasionally shaking the films. Maybe it
does not save that much time but it seems like it late at night.

Ron

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear All,
} Just wondering if anyone out there uses a nitrogen bubble system to agitate
} their film processing solutions in tanks. We are considering such a system
} but I would like a clearer idea about the set-up before going ahead and
} asking our workshop to make the system. At present we use Kodak 4489 film
} and ordinary tanks with Kodak D19 developer and Ilford Hypam, this works
} fine except occasionally when people forget to agitate the film.
}
} If anyone has such a system which is being used for sheet negs I would
} appreciate a rough description how it works. ie
}
} 1) what the layout of tubes on the bottom of the tanks is like
}
} 2) what kind of control system is used to set the bubbling intervals
} (presumably something like a solenoid on a timer connected through a
} regulator on to a N2-filled cylinder?)
}
} Are there any problems or drawbacks to the systems which are in use? Are
} they worth the trouble of setting up and maintaining?
}
}
} Best regards,
}
} Richard
}
}
} Richard Easingwood
} South Campus Electron Microscope Unit
} School of Medical Sciences, University of Otago
} PO Box 913, Dunedin
} NEW ZEALAND
}
} Telephone: 64-03-479 7301 Facsimile: 64-03-479 7254
} mailto:richard.easingwood-at-stonebow.otago.ac.nz
} http://anatomy.otago.ac.nz:800/Department/EMUnit.html
} http://www.otago.ac.nz/anatomy/emunit/
}
}
}
}
}
}

===========================================================================
Mr. Ron Doole e-mail ron.doole-at-materials.ox.ac.uk
Department of Materials, phone +44 (0) 1865 273701
University of Oxford, fax +44 (0) 1865 283333
Parks Road.
Oxford. OX1 3PH. UK.
============================================================================




From daemon Tue Jul 25 06:57:41 2000



From: Roger Moretz :      rcmoretz-at-excite.com
Date: Tue, 25 Jul 2000 04:45:37 -0700 (PDT)
Subject: Re: image database software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Just an additional comment on this. Late '99 we evaluated several database
packages: ImageCentral from Advanced Imaging Concepts (Princeton, NJ),
ElectroImage (ElectroImage, Great Neck, NY) and Archive4Images (Long Island
Instruments, East Meadow, NY). Each system has its pros and cons. The
ElectroImage database package has since been upgraded with Java and Web
enabling; ImageCentral is a network-enabled package; Archive4Images includes
some processing tools. ElectroImage is an outstanding product, but has a
limited number of fields for searching, whereas ImageCentral has a much
larger number of fields--the single feature that the Experimental Pathology
group found to be most important. None of these packages is
inexpensive--expect $1500 to $4K for cost of the database alone. If cost is
not a factor, check out these systems. Of course, you can always develop
your own using Paradox or Access. On the non-scientific side, check out the
current version of ULead's ImagePals--I have been using this at home since
v.2 came out a very long time ago.

Roger Moretz, Ph.D.
Dept of Toxicology
BI Pharmaceuticals, Inc.

I have no interest in any of the products, vendors or suppliers other than
to have evaluated or used the product(s) somewhere in my life.
On Fri, 21 Jul 2000 12:46:21 -0500, default wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I am tasked with setting up an electronic library of SEM images, and I'm
} looking for good database software applications for cataloging and
} viewing images. Multi-user access and strong software technical support
} are primary requirements. What are my options?
}
}


Roger C Moretz, Ph.D.





_______________________________________________________
Say Bye to Slow Internet!
http://www.home.com/xinbox/signup.html




From daemon Tue Jul 25 08:59:56 2000



From: Neelima Shah :      shahn-at-mail.med.upenn.edu
Date: Tue, 25 Jul 2000 09:50:00 -0400
Subject: antibody

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Anyone out there know of any antibody that would be specific for mouse
endothelial cells?? We are trying to identify endothelial cells in tumors
as well as cultured endothelial cells. I know a lot of them work for LM
after various treatments. I seem to have had no luck so far staining at EM
level. I would appreciate all the input.Thanks in advance.....
Neelima Shah..............
Biomedical Imaging Core Facility
Uni of Pennsylvania
Philadelphia, Pa.




http://www.MED.upenn.edu/morphlab/




From daemon Tue Jul 25 09:05:01 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Tue, 25 Jul 2000 08:51:58 -0500
Subject: Administrivia: Reminder Listserver rules specify No Selling....

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Colleagues

There has been a creeping effect in postings over time where
individuals are listing items "for sale" on the listserver.

Let me once again remind you all of our rules (from
the FAQ sheet you ALL received) that this is not permitted and it
applies to everyone (including Universities) not just Commerical
organizations.

The general public does not know but every posting that I see (I do miss some)
that breaks the Listserver rules generates a PRIVATE warning from me.
If the transgressions continue it will culminate with exclusion (ie.
filtering/banning)
of an individual's privilege of posting to the Listserver.
For the record, nearly a dozen individuals have been barred from posting
(but they may still receive mail) for extended periods.

Returning to the subject of providing a forum for listing/offering used
equipment
it is obvious that the community needs an electronic forum for this function.

To this end, I will create a WWW site which will allow this and will
announce it as soon as it is operational.

It will be seperate from the Listserver and will be purely WWW based.
The model for this is the Vendor news site which I created over a year ago
and continues to operate successfully.

(http://www.msa.microscopy.com/News/NewsListings.html)


Cheers...

Nestor
Your Friendly Neighborhood SysOp

----------------------------------------------------


Exerpt from the Microscopy Listserver Rules
----------------------------------------------------
Can I post an Advertisement / Commerical / Marketing Survey Message(s)?
---------------------------------------------------

No, that does not fit within the bounds of this discussion forum.

This listserver is not intended to be a Sales/Marketing/Survey mechanism
for organizations, but rather it is an open discussion area about
microscopy and microanalysis problems and solutions.

If you are an organization and have equipment you wish to donate, or sell,
for nominal cost (i.e. no profit) then this is generally an acceptable
posting. If you are not sure then send a copy of the announcement in
question to Zaluzec-at-MSA.Microscopy.Com and I will give you my opinion. An
example
of this type would be an old decommissioned instrument which someone is
trying to give away for removal/shipping costs, that would fit within the
bounds of the purposes of this list.

If you are interested in using the Internet for Commerical Advertising of
Microscopy/Microanalysis Related Products/Services, you may wish to contact
MSA at it's WWW site (http://www.msa.microscopy.com) or the MSA Business
Office (MSABusinessOffice-at-MSA.Microscopy.Com). These alternative Internet
services, are provided independently of the Listserver Operation, which MSA
provides as a FREE service to the WorldWide
Microscopy and Microanalysis Community. Any funds derived from the above
are used to defray the costs of running MSA's Internet site.

If you wish to purchase a copy of the MSA Membership directory/Mailing list
please contact the MSA Business Office .

There is a mechanism to post Commerical NEWS to the Microscopy Community.
If you have something of a purely Commerical Nature and wish to make it
known to the community you may FREELY use the following WWW site (just
follow the on-line instructions)

http://www.msa.microscopy.com/News/NewsListings.html





From daemon Tue Jul 25 09:13:22 2000



From: Marie E. Cantino :      cantino-at-uconnvm.uconn.edu
Date: Tue, 25 Jul 2000 10:06:58 -0400
Subject: Re: Nitrogen developer agitation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Richard,

We have a nitrogen burst system that we use for the developing tank. It
consists of an open metal rack with five metal tubes at the bottom, each
with a series of tiny holes spaced about 1-1.5 cm apart and facing the
bottom of the tank. Our plexiglass negative rack fits inside the gas rack,
and both fit in our 9x5.5 inch developing tank. The rack is connected to
a nitrogen tank via a burst regulator (Arkay Corp.) with variable burst
frequency and duration.

One caution: about a year ago we noticed that our developed negatives were
coming out uneven, even when they were exposed to an absolutely uniform
electron beam. On examination, some of the holes were found to be
encrusted with metal salts. Once cleaned out, the negatives became uniform
again. This was only after many (15+) years of operation, so it isn't a
big problem if you watch out for it.

Marie

Dr. Marie E. Cantino
Dept. of Physiology and Neurobiology, U-2131
University of Connecticut
Storrs, CT 06269-2131
Phone: 860-486-3588
Fax: 860-486-1936





From daemon Tue Jul 25 10:01:01 2000



From: Giles, Bill :      William.Giles-at-TIMET.com
Date: Tue, 25 Jul 2000 08:49:04 -0600
Subject: Polaroid Film Choices

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Howdy List,

I need to stop using Polaroid T-55 p/n sheet film in my lab(good bye old
friend). The enviormental guys got to us.

The question is, what to replace it with? T-52, T-53 or T-54.

We will be using it primarily in our old Cambridge 250, inverted
metallograph, and some macro stand usage.

Its use will be limited as our new JEOL SEM and inverted metallograph are
going digital.

Any suggestions?


William T. Giles
Sr. Electron Microscopist
Met. Lab. Coordinator
Henderson Technical Laboratory
TIMET
PO Box 2128 Henderson NV 89009
Ph: (702)566-4436
Fax: (702)564-9038
E-mail: Bill.Giles-at-timet.com




From daemon Tue Jul 25 11:01:30 2000



From: Marie E. Cantino :      cantino-at-uconnvm.uconn.edu
Date: Tue, 25 Jul 2000 11:53:02 -0400
Subject: Re: Polaroid Film Choices

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Since we (and probably others) use this film, I'm curious what is the
hazardous/toxic part. Is it the Na sulfite solution, the goop on the film,
or the coater?

Marie


Dr. Marie E. Cantino
Dept. of Physiology and Neurobiology, U-2131
University of Connecticut
Storrs, CT 06269-2131
Phone: 860-486-3588
Fax: 860-486-1936





From daemon Tue Jul 25 17:36:11 2000



From: Sarah Peters :      petersar-at-ohsu.edu
Date: Tue, 25 Jul 2000 15:20:20 -0700
Subject: Tips on flat embedding with LR White

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I would appreciate any hints, tips, or procedures available on flat embedding using LR White.
Thanks!
S. Peters

Sarah Peters NRC04
Oregon Hearing Research Center
3181 SW Sam Jackson Pk Rd
Portland, Or. 97202
Ph:(503) 494-2942
Fax: (503) 494-5656





From daemon Tue Jul 25 18:44:03 2000



From: Ekstrom, Harry :      harry.ekstrom-at-honeywell.com
Date: Tue, 25 Jul 2000 16:32:25 -0700
Subject: RE: Polaroid Film Choices

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I think both. I know on the pieces of film, there was some kind of warning
about getting the goop (Na sulfite) on your skin. Before we went to the
coaterless film (type 53) way back when, we had a special hazmat can for the
coaters.

Now we're happily digital.

Harry Ekstrom
Materials Laboratory

(602) 231-2744
e-mail: harry.ekstrom-at-honeywell.com








From daemon Tue Jul 25 18:57:52 2000



From: Marek Malecki :      mmm-at-ucsd.edu
Date: Tue, 25 Jul 2000 16:52:37 -0700
Subject: photoconversion of DAB

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Would anyone have a wavelength sensitivity curve for photoconversion of
diamonobenzidine?



M. Malecki, M.D., Ph.D.
Director and Principal Investigator

Molecular Imaging Laboratories
University of California at San Diego
address: 1500 Suite Bonner Hall, 9500 Gilman Drive, La Jolla, CA 92093-0368
phone: 8588223373
fax: 8588223715
pager: 8586161420
e.mail: mmm-at-ucsd.edu
e.pager: 1620024619-at-alphapage.airtouch.com
www site: http://mil.ucsd.edu/
ftp site: mil1.ucsd.edu






From daemon Tue Jul 25 22:03:14 2000



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Tue, 25 Jul 2000 23:00:28 -0500
Subject: Polaroid film and skin sensitivities

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Harry Ekstrom wrote:
============================================================
I think both. I know on the pieces of film, there was some kind of warning
about getting the goop (Na sulfite) on your skin. Before we went to the
coaterless film (type 53) way back when, we had a special hazmat can for the
coaters.
============================================================
Over the thirty plus years we have used the Polaroid P/N film , the type
that took the sodium sulfite clearing solution (but we hardly use any today)
, it was my recollection that we had cases of what seemed like contact
dermatitis from more than several employees, with some being sensitive to
the chemicals in the coaters. The sodium sulfite solution seemed to be a
mild irritant to others. I don't recall anyone being affected by the
chemicals in the composite film pack, but then again, the strong warning
might have given everyone a special "heads up". But I would avoid having
any of these kinds of chemicals come in contact with your skin.

Another thing I found out is that one can develop a sensitivity to at least
the coater chemicals, that is one might not be initially reactive to them,
but with time, one could develop a sensitivity to them.

Disclaimer: Digital microscopy has put an almost complete stop to the sales
of these kinds of film products.

Chuck
============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.spi.cc
########################
============================================





From daemon Wed Jul 26 04:41:47 2000



From: Michael Reiner :      Elektronenmikroskopie-at-web.de
Date: Wed, 26 Jul 2000 11:31:48 +0200
Subject: Re: antibody

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Neelima Shah!

Did you try PECAM-1/CD31 (rat) from Pharmingen? It must work well at least using a pre-embedding protocol without permeabilization.

Greetings,
Michael Reiner

University of Cologne, Germany
Department of Anatomy I



} Anyone out there know of any antibody that would be specific for mouse
} endothelial cells?? We are trying to identify endothelial cells in tumors
} as well as cultured endothelial cells. I know a lot of them work for LM
} after various treatments. I seem to have had no luck so far staining at EM
} level. I would appreciate all the input.Thanks in advance.....
} Neelima Shah..............
} Biomedical Imaging Core Facility
} Uni of Pennsylvania
} Philadelphia, Pa.
}
}
}
}
} http://www.MED.upenn.edu/morphlab/
}
} è


_______________________________________________________________________
1.000.000 DM gewinnen - kostenlos tippen - http://millionenklick.web.de
IhrName-at-web.de, 8MB Speicher, Verschluesselung - http://freemail.web.de




From daemon Wed Jul 26 06:55:10 2000



From: User on lab1 :      lab1-at-ccnet.up.ac.za
Date: Wed, 26 Jul 2000 13:44:25 +0200
Subject: Thermophile bacteria

Contents Retrieved from Microscopy Listserver Archives
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Hello Microscopists
I have a client who wants to image (SEM) thermophile bacteria in situ in
a bioleaching environment. Any one have any experience in this regard?
We have tried the conventional prep procedure: .2u filter GA-----CPD,
but with no luck.
Any pointers will be appreciated

Thank you

Alan




From daemon Wed Jul 26 09:12:35 2000



From: Tamara Howard :      howard-at-cshl.org
Date: Wed, 26 Jul 2000 09:41:48 -0400 (EDT)
Subject: Re: Tips on flat embedding with LR White

Contents Retrieved from Microscopy Listserver Archives
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How large are your samples? Are you going to do UV cure or heat? I have
procedures for embedding cells on coverslips as well as "normal" samples
that need flat-embedding....let me know which samples you have!

Tamara Howard
CSHL


On Tue, 25 Jul 2000, Sarah Peters wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I would appreciate any hints, tips, or procedures available on flat embedding using LR White.
} Thanks!
} S. Peters
}
} Sarah Peters NRC04
} Oregon Hearing Research Center
} 3181 SW Sam Jackson Pk Rd
} Portland, Or. 97202
} Ph:(503) 494-2942
} Fax: (503) 494-5656
}
}
}
}




From daemon Wed Jul 26 09:36:13 2000



From: Debby Sherman :      sherman-at-btny.purdue.edu
Date: 26 Jul 2000 09:24:45 -0500
Subject: M&M Facility Management

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------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


To all you struggling managers of multi-user or service facilities:

This is a reminder that there will be a special "EXPERTS" session to discuss topics of interest in this area. The session will be help from 9:00 to 12:00 on Wednesday, August 16 in Room 106.

Format of the session will be as follows:
1) 3 topics recommended by you will be initially presented with each introduced by an expert on that topic.
2) The order of the discussion will be announced in advance so those of you with conflicts will have an idea of when to attend this session.
3) The initial introduction of a topic will be short (5-10min) so as to leave maximum time for questions and discussion from attendees.
4) We will try to limit open discussion on any one topic to no more than 30min so that all 3 topics can be discussed in approximately a 2 hr. time period. The remaining time can be used to revisit one of the topics or introduce new topics from the floor for discussion.

In an earlier E-mail, I requested suggestions for topics be sent to me. Of the ones received, those listed below seemed to be the most popular.

1. Maintenance of equipment: service contracts ; service contracts vs. third-party vs insurance company or self-insure; timely service

2. Justification of costs/ cost recovery….electronic bookkeeping and billing

3. login systems for instruments/lab security/training

You will notice that they are quite broad and may have to be narrowed down by the "expert" introducing them in order to have a meaningful discussion. I am now looking for the "experts" to introduce topics 1 and 2. Keith Darlington from Elf ATOCHEM North America, Inc has gratiously consented to introduce topic #3. Please E-mail me if you would like to introduce topics 1 or 2 or can recommend someone to do so.

Also, if any of you have information relating to the above topics that can be made available to those attending, please bring handouts or send the info to me and I will have it copied for distribution.

There has been requests to record the session so that those not in attendance can benefit from the discussions. I will try to arrange this if appropriate recording equipment is available.

One final note...I will not arrive at the meetings until Monday so would appreciate a volunteer to put up notices about the session throughout the convention hall on Sunday. Please contact me if you are willing to do this and I will get the notices to you.

Many thanks and PLEASE send me names of individuals I can ask to introduce topics. Also, if you have suggestions about the format of the session, please contact me...there is still plenty of time to modify it.

Debby

Debby Sherman, Manager Phone: 765-494-6666
Microscopy Center in Agriculture FAX: 765-494-5896
Dept. of Botany & Plant Pathology E-mail: sherman-at-btny.purdue.edu
Purdue University
1057 Whistler Building
West Lafayette, IN 47907-1057




From daemon Wed Jul 26 09:37:39 2000



From: Giles, Bill :      William.Giles-at-TIMET.com
Date: Wed, 26 Jul 2000 08:26:38 -0600
Subject: RE: Polaroid Film Choices

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id {NJ3XTDDN} ; Wed, 26 Jul 2000 08:31:53 -0600
Message-ID: {90F2CBD1ADD9D3119A3A00A0C9A94BA302E770-at-henexc1.timet.com}
{Microscopy-at-sparc5.microscopy.com}


We must obtain and pay for a permit for any item put into our process waste
stream. Any item not permitted, is considered a "hazardous" waste, and must
be disposed of at great cost.

Also, all waste water must either be ponded or reclaimed, this is now
becoming a large expense for our facility.

Our enviormental department examined each departments waste stream and in
cooperation with the individual departments (thank goodness) made some
determinations as to where we could save not only permit costs but water
usuage.

The processing of the p/n film uses a rather significant amount of water for
my department, not to mention the occassional print production.

Also, you can check Polaroid's site for it's MSDS sheets for all it's
products.http://www.polaroid.com/service/msds/index.html

One positive outcome of this, my request for a new metallograph with a high
end digital camera has been approved!

Anyway, that's the basic scenario. No Federales came in and spanked us,
rather it was a cost-saving(??) measure implemented by our enviormental
group.

Back to my original question: What type of Polaroid do you all use?

William T. Giles
Sr. Electron Microscopist
Met. Lab. Coordinator
Henderson Technical Laboratory
TIMET
PO Box 2128 Henderson NV 89009
Ph: (702)566-4436
Fax: (702)564-9038
E-mail: Bill.Giles-at-timet.com

} -----Original Message-----
} From: Marie E. Cantino [SMTP:cantino-at-uconnvm.uconn.edu]
} Sent: Tuesday, July 25, 2000 8:53 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Re: Polaroid Film Choices
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Since we (and probably others) use this film, I'm curious what is the
} hazardous/toxic part. Is it the Na sulfite solution, the goop on the
} film,
} or the coater?
}
} Marie
}



From daemon Wed Jul 26 10:09:02 2000



From: sherwood-at-helix.mgh.harvard.edu (Peggy Sherwood)
Date: Wed, 26 Jul 2000 10:58:32 -0400
Subject: Nitrogen Burst System

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Dear Richard, et al:

We have been using an old model Leedal Sink with nitrogen burst system for
about 12-14 years. Other than having the temperature gauge (which
regulates the temperature of the developer) replaced, the system has been
great! It has metal tanks that have metal rods with holes on the bottom
(where the nitrogen comes through). You regulate the duration of the burst
and the interval between the bursts with a timer built into the sink.
Hoses connect the tanks to the nitrogen tank. The developing and fixing
tanks float in water that is temperature-regulated. This system gives
good, even distribution of the chemicals over the negatives.

(The best part is I got this refurbished sink, racks, darkroom lights, etc.
for $750.00! Can't complain.)

Peggy Sherwood
MGH-Wellman Labs of Photomedicine (W224)
55 Fruit Street
Boston, MA 02114

617-724-4839 (voice mail)
617-726-6983 (Photopathology Lab)
616-726-3192 (fax)






From daemon Wed Jul 26 10:23:30 2000



From: Arun Kumar Subramanian :      ksu973-at-merle.acns.nwu.edu
Date: Wed, 26 Jul 2000 10:11:53 -0500 (CDT)
Subject: TEM : Film

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Dear listers,
We have been using AGFA scientia 23D56 (3.25x4 ") film for years now until
AGFA discontinued that product. I would greatly appreciate any help in
finding a suitable replacement for this film

thanks
--arun

___________________________________
Arun Kumar Subramanian
Graduate Student
Dept of Materials Science & Engg




From daemon Wed Jul 26 13:12:37 2000



From: S.C.Hogg :      S.C.Hogg-at-sheffield.ac.uk
Date: Wed, 26 Jul 2000 18:58:25 +0100
Subject: TEM dislocation density measurment in Al

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,

I need to measure the dislocation density in an Al-30Si alloy.

I've been told there is a condition that will give the optimum
dislocation contrast which will give some proportion of the total
dislocations.

Does anyone know what these conditions are and the proportion
that this gives (and a reference if possible?)

Also any comments on other methods that don't require one to
measure the thickness of the sample e.g. simply counting the
intersections of dislocations with the surface and dividing by the
area?

Thank-you,

Simon Hogg


University of Sheffield
Department of Engineering Materials
Sir Robert Hadfield Building
Mappin Street
Sheffield
S1 3JD
Tel. 0114 222 5934
Fax. 0114 222 5943



From daemon Wed Jul 26 14:28:52 2000



From: Jen Delille :      jen-at-sl3d.com
Date: Wed, 26 Jul 2000 13:20:11 -0600
Subject: Flourescent stains- Microscope slides

Contents Retrieved from Microscopy Listserver Archives
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Where can I find prepared microscope slides that use flourescence? Or, where
can I find information about how to prepare my own slides with flourescent
stains? I would like such samples for research on 3D microscopy.

Sincerely,

Jennifer Delille

jen-at-sl3d.com




From daemon Wed Jul 26 16:49:37 2000



From: michael shaffer :      mshaf-at-darkwing.uoregon.edu
Date: Wed, 26 Jul 2000 14:36:01 -0700
Subject: RE: Polaroid Film Choices

Contents Retrieved from Microscopy Listserver Archives
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William T. Giles writes ...

} Back to my original question: What type of Polaroid do you all use?

Oh yea ... the question.

Type 52 is what we use when we aren't grabbing digital ... but even
then, printing four 1024x800s to the dye-sub proves more cost
effective ... needless-to-say, my type 52 is all past its use-by date.

Type 52 is also ASA400, so you will have to re-calibrate your
exposure. It also doesn't require the smelly "goop".

shAf :o)




From daemon Wed Jul 26 19:33:46 2000



From: Gary Gaugler :      gary-at-gaugler.com
Date: Wed, 26 Jul 2000 17:22:31 -0700
Subject: SEM spot size & x-ray analysis

Contents Retrieved from Microscopy Listserver Archives
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I've studied Goldstein's book to try and accurately compute
the true spot size of a SEM beam based on the myriad of
factors which influence it. At issue is how to determine the
actual spot size of a beam, in microns, at the specimen.

SEMs from various makers use relative numbers for spot
size. These indicate the relative size of the beam from
"really small" to "largest." "Really small" means low
specimen current but high resolution and the converse
for the large spot size. If one is doing x-ray analysis,
the spot size may or may not be an issue.

If the area being analyzed is large, the spot size is
most likely not all that important. But for the analysis
of small feature size and sub-micron semiconductor devices,
true spot size is an issue. If the beam covers more than
the area of interest, the x-ray data will not accurately
reflect the composition of the intended material.

Therefore, is there some basic limitation of the applicability
of quantitative SEM x-ray analysis based on the size of
the specimen? If so, what is this limit? Also, is there
some way to accurately define the physical size of a
beam at the specimen? If so, what is this method?

Any thoughts on this?

gary g.




From daemon Wed Jul 26 22:15:10 2000



From: Gina Sosinsky :      gsosinsky-at-ucsd.edu
Date: Wed, 26 Jul 2000 20:05:24 -0700
Subject: Tutorial Sessions for MSA meeting 2000

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Dear Colleagues,

For those of you planning on attending the Microscopy Society of America in
Philadelphia August 13-17 (coming up in a few weeks), I wanted to alert you
to the following program setup.

***** Please note: due to a scheduling snafu, David DeRosier's talk will be
in the morning whereas the other two talks are scheduled for the afternoon
session *****

There are 3 biological tutorials being presented:

David DeRosier: DeRosier's Guide to Three-Dimensional Reconstruction of
Helical Structures
Tuesday 11:00 AM Room 103

Liz Wilson-Kubalek: Structural Analysis of Proteins on Lipid Substrates
Tuesday 3:00 PM Room 103

Steve Ludtke: Single Particle Analysis of Macromolecules and Complexes: How
to Get Started
Tuesday 4:00 PM Room 103

Sincerely,
Gina Sosinsky
Session Chair for Biological Sciences Tutorial

P.S. For those of you who yearn to go beyond electrons, there is also a
joint biological/physical sciences tutorial being given by Dave Piston from
Vanderbilt University on Multi-Photon Excitation Microscopy: An Old Idea
in Quantum Theory Applied to Modern Scientific Problems.
Monday 3:00 PM Room 103
*****************************************
* Gina Sosinsky, PhD. *
* University of California at San Diego *
* 365 San Diego Supercomputer Center *
* 9500 Gilman Drive *
* La Jolla, CA 92093-0505 *
* *
* Note new area codes: *
* 858-534-6264 (office phone & *
* voice mail) *
* 858-534-4583 (lab phone) *
* 858-822-0861 (fax) *
* gsosinsky-at-ucsd.edu (email) *
*****************************************



From daemon Thu Jul 27 01:16:56 2000



From: Michael Reiner :      Elektronenmikroskopie-at-web.de
Date: Thu, 27 Jul 2000 08:03:49 +0200
Subject: Re: TEM : Film

Contents Retrieved from Microscopy Listserver Archives
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Hello Arun Kumar Subramanian,

You can replace the Agfa Scientia Film with Kodak´s SO-163 EM Film without any Problems.
Greetings,
Michael


Michael Reiner
University of Cologne, Germany
Dept. of Anatomy I
_______________________________________________________________________
1.000.000 DM gewinnen - kostenlos tippen - http://millionenklick.web.de
IhrName-at-web.de, 8MB Speicher, Verschluesselung - http://freemail.web.de




From daemon Thu Jul 27 01:52:39 2000



From: Gordon Couger :      gcouger-at-couger.com
Date: Thu, 27 Jul 2000 01:41:55 -0500
Subject: Re: SEM spot size & x-ray analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} From: "Gary Gaugler" {gary-at-gaugler.com}
}
} I've studied Goldstein's book to try and accurately compute
} the true spot size of a SEM beam based on the myriad of
} factors which influence it. At issue is how to determine the
} actual spot size of a beam, in microns, at the specimen.
}
} SEMs from various makers use relative numbers for spot
} size. These indicate the relative size of the beam from
} "really small" to "largest." "Really small" means low
} specimen current but high resolution and the converse
} for the large spot size. If one is doing x-ray analysis,
} the spot size may or may not be an issue.
}
} If the area being analyzed is large, the spot size is
} most likely not all that important. But for the analysis
} of small feature size and sub-micron semiconductor devices,
} true spot size is an issue. If the beam covers more than
} the area of interest, the x-ray data will not accurately
} reflect the composition of the intended material.
}
} Therefore, is there some basic limitation of the applicability
} of quantitative SEM x-ray analysis based on the size of
} the specimen? If so, what is this limit? Also, is there
} some way to accurately define the physical size of a
} beam at the specimen? If so, what is this method?
}

Gary,

It was a good deal different set up but the idea should be the same. We
were working with a scanning gamma ray device and the resolution was
measured in hundredths of an inch but we built test samples that we knew
the
size of the metal slug and we knew how far it was between them. We would
do a scan and see if we could resolve them.

It would be a lot tougher to build the model for what you want to do than
for us. We used drill bits in Plexiglas. But if you coat various spacing
grids with something that shows up in your X-ray scans and then polish the
peaks off you should be able to get islands of various sizes and spacing
to do your calibration.

We were primarily using gamma rays for image reconstruction using a Raydon

transform but we did some work with fluoresced x-rays. The gamma beam was
about 3/32 of an inch. The customer did some direct imaging of Plutonium
intrusion into various rocks with it as well. Better him than me.

The device was a gamma source and a detector with the subject on a rotary
table that would move in the X and Z axis.

Gordon
Gordon Couger gcouger-at-couger.com
Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00











From daemon Thu Jul 27 01:54:46 2000



From: Witold Zielinski :      WIZIEL-at-inmat.pw.edu.pl
Date: Thu, 27 Jul 2000 08:50:25 +0000
Subject: Re: TEM dislocation density measurment in Al

Contents Retrieved from Microscopy Listserver Archives
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*From: "S.C.Hogg" {S.C.Hogg-at-sheffield.ac.uk}
*To: Microscopy-at-sparc5.microscopy.com
*Date sent: Wed, 26 Jul 2000 18:58:25 +0100
*Subject: TEM dislocation density measurment in Al
*Priority: normal

*------------------------------------------------------------------------
*The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Hello,

It seems to me that the proportion of the total dislocation
density which are people talking about is very rough estimate.
But for sure two beam condition is the proper one to visualise
dislocations. So, if you want to be acurate try to obtain an image of
dislocation structure in the same area using two perpendicular g
vectors, for example in the [001] zone axies.
Your accuracy in dislocation density estimate depends also on
deformation mode like slip systems involved, cross slip etc. so
using more g vectors you'll be obtaing higher accuracy.

Good luck,

Witold Z.

*
*Hello,
*
*I need to measure the dislocation density in an Al-30Si alloy.
*
*I've been told there is a condition that will give the optimum
*dislocation contrast which will give some proportion of the total
*dislocations.
*
*Does anyone know what these conditions are and the proportion
*that this gives (and a reference if possible?)
*
*Also any comments on other methods that don't require one to
*measure the thickness of the sample e.g. simply counting the
*intersections of dislocations with the surface and dividing by the
*area?
*
*Thank-you,
*
*Simon Hogg
*
*
*University of Sheffield
*Department of Engineering Materials
*Sir Robert Hadfield Building
*Mappin Street
*Sheffield
*S1 3JD
*Tel. 0114 222 5934
*Fax. 0114 222 5943
*
*
%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
Witold Zielinski, Ph.D.
Warsaw University of Technology
Department of Materials Science and Engineering
02-507 Warszawa, Woloska 141
POLAND

phone #: /48 22/ 660 84 41
fax #: /48 22/ 848 48 75



From daemon Thu Jul 27 03:21:26 2000



From: rlvaughn-at-unmc.edu
Date: Wed, 26 Jul 2000 16:46:41 -0500
Subject: Job Posting TEM

Contents Retrieved from Microscopy Listserver Archives
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I was asked before I leave to post this job opening. It's been great
reading about all our trials and tribulations. I have probably ran into
some of you at the meetings which were always fun. Dr. Rodriguez-Sierra is
attempting to go to the meetings to place an advertisement at the Forums'
booth. Thanks for all the help in the past.

Rick Vaughn

The Electron Microscopy Core Research Facility at the University of
Nebraska Medical Center (UNMC) in Omaha, NE has an opening for a Biomedical
EM Technologist to direct the laboratory and support research projects that
involve transmission electron microscopy. The laboratory is well equipped
and has a history of excellent productivity and adequate funding.

The successful candidate for this position will possess at least a
Bachelor's degree in Biology with experience in electron microscopy.
Additional courses or experience in immunocytochemistry, Cell Biology, and
digital imaging is desirable. Operating knowledge of transmission electron
microscopes is preferred. The applicant must have excellent communicative
skills and the ability to work well with a variety of personalities.

The EM technologist interacts with all laboratory users in order to
accomplish specific research goals with respect to preparation for a
variety of biomedical samples for TEM, operation of TEM, darkroom
developing and printing, digital image capture and reporting. The
technologist will also assist the faculty in the laboratory section of EM
graduate courses.

UNMC offers a competitive salary and benefits package. UNMC is a equal
opportunity employer.


If interested, please submit a cover letter and resume to:
Dr. Jorge F. Rodriguez-Sierra
Department of Cell Biology and Anatomy
University of Nebraska Medical Center, Omaha, NE 68198-6395
Fax: 402-559-7328
Phone 402-559-6259
e-mail: jrodrigu-at-unmc.edu




From daemon Thu Jul 27 03:23:17 2000



From: emitech :      em-at-emitech.demon.co.uk
Date: Thu, 27 Jul 2000 09:16:37 +0100
Subject: Re: TEM : Film

Contents Retrieved from Microscopy Listserver Archives
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Dear Arun,

We have been recommended Kodak SO-163 3 1/4" x 4" Film to replace, it
has a similar speed, transfer to this type should be accomplished with
minimal changes to exposure and development. Alternatively the Kodak
4489 which is slower film, which has been widely used for biological
applications where high contrast is desirable. we can supply if required
Boxes of 100, please contact off line.

Kind Regards,


Christine Fitzgerald

In message {Pine.HPP.3.93.1000726100957.15489F-
100000-at-merle.acns.nwu.edu} , Arun Kumar Subramanian
{ksu973-at-merle.acns.nwu.edu} writes
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

--
emitech



From daemon Thu Jul 27 06:40:39 2000



From: Joergen Bilde-Soerensen 5802 :      j.bilde-at-risoe.dk
Date: Thu, 27 Jul 2000 13:29:09 +0200
Subject: Re: TEM dislocation density measurment in Al

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Simon Hogg wrote:
} I need to measure the dislocation density in an Al-30Si alloy.
}
} I've been told there is a condition that will give the optimum
} dislocation contrast which will give some proportion of the total
} dislocations.
}
} Does anyone know what these conditions are and the proportion
} that this gives (and a reference if possible?)
}
} Also any comments on other methods that don't require one to
} measure the thickness of the sample e.g. simply counting the
} intersections of dislocations with the surface and dividing by the
} area?

Hi Simon,

There are a number of problems to consider if you want to measure
dislocation density by TEM. (i) The dislocations may rearrange during
preparation of the thin foil because of image forces. (ii) Some of the
dislocations may be out of contrast under the diffraction conditions
used. (iii) There may be a preponderance of certain Burgers vectors. (iv)
The line vectors may not be evenly distributed in space.

I think most dislocation density measurements have been made under
some simplifying assumptions that make them - as Witold Zielinski wrote
- very rough estimates. Images are taken with a given two-beam
condition. If, for instance, the material is fcc and the reflection is 200, it is
then assumed that 1/3 of the dislocations will be out of contrast for this
reflection. It is further assumed that the stereological equation (which
assumes a random line vector distribution) is valid. A result obtained
under such assumptions is not necessarily very exact.

As Witold wrote, you can try to image all dislocations by taking several
images under different diffraction conditions. (You can also do this in a
single image with a hollow-cone technique). This still leaves you with
points (i) and (iv).

With respect to (i) you will e.g. in pure Al find that the dislocation
density varies with foil thickness because relatively more dislocations
are lost from the thinner parts of the foil. In other words use as thick a
foil as possible.

With respect to (iv): the method that requires measurement of the foil
thickness gives you the advantage that you can count intersections with
lines of various orientations (or with a circle). If you count intersections
with the surface, you have to assume that the line vectors are evenly
distributed in space (consider the extreme case that all dislocation lines
are parallel to the surface).

Instead of counting individual dislocations in a TEM it is also possible
to measure line broadening in selected area channelling patterns in a
scanning microscope or in X-ray diffractograms.

Best regards,
Jorgen



*****************************
J. B. Bilde-Soerensen
Senior Research Scientist, Ph. D.
Materials Research Department
Risoe National Laboratory
DK-4000 Roskilde
Denmark

e-mail: j.bilde-at-risoe.dk
phone: +45 46 77 58 02 (direct)
phone: +45 46 77 46 77 (switchboard)
fax: +45 46 77 57 58
website: http://www.risoe.dk/afm/Personal/jqbi/jqbi.htm



From daemon Thu Jul 27 07:25:58 2000



From: White, Woody N :      Woody.N.White-at-mcdermott.com
Date: Thu, 27 Jul 2000 07:59:00 -0500
Subject: Re:SEM spot size & x-ray analysis

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Gary,

The beam size plays an important role in determining the size of a particle
or
feature one can analyze. Obviously the beam diameter must be smaller than
the
location to be analyzed and typically larger beam diameters indicate hagher
current and count rates. The real determinant of what size can be analyzed
without influence from surounding material is what "analysis volume" size is
generated. The diameter of the analysis volume will never be smaller than
the
beam. It is usually much larger due to scattering within the specimen.
Parameters which influence the analysis volume size and shape include the
composition of the material being analyzed, the surface condition of the
specimen, the angle of the incident beam, and the potential of the beam
(kV).
Hope I didn't forget one... If I recall correctly, Goldstein, et al, does
cover this effect in the book.

Don Chernoff has available (http://www.small-world.net/efs.htm) a program
which
models the analysis volume. A similar but less flexable program came with
my
(former) Kevex EDS in the mid 80s. I have not tried the EFS software, nor
have
I any ties to small-world.

Woody White
McDermott Technology, Inc.



From daemon Thu Jul 27 07:51:27 2000



From: George Laing :      scisales-at-ngscorp.com
Date: Thu, 27 Jul 2000 08:43:21 -0700
Subject: RE: Nitrogen developer agitation

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Arkay makes an affordable gas burst controller as well as water jacket/
tank systems for sheet film developing. Leedal also produces excellent
jacket/tank systems. Both are available with gas burst plenums as
required.
Neither Leedal or Regal/Arkay has an active website that I could find.
I will forward information to anyone who wishes to contact me directly.

George Laing
National Graphic Supply
scisales-at-ngscorp.com

} Dear All,
} Just wondering if anyone out there uses a nitrogen bubble system to
agitate
} their film processing solutions in tanks. We are considering such a
system
} but I would like a clearer idea about the set-up before going ahead and
} asking our workshop to make the system.
}
} If anyone has such a system which is being used for sheet negs I would
} appreciate a rough description how it works. ie
}
} 1) what the layout of tubes on the bottom of the tanks is like
}
} 2) what kind of control system is used to set the bubbling intervals
} (presumably something like a solenoid on a timer connected through a
} regulator on to a N2-filled cylinder?)
}
} Are there any problems or drawbacks to the systems which are in use? Are
} they worth the trouble of setting up and maintaining?
}
}
} Best regards,
}
} Richard
}
}
} Richard Easingwood
} South Campus Electron Microscope Unit
} School of Medical Sciences, University of Otago
} PO Box 913, Dunedin
} NEW ZEALAND
}
} Telephone: 64-03-479 7301 Facsimile: 64-03-479 7254
} mailto:richard.easingwood-at-stonebow.otago.ac.nz
} http://anatomy.otago.ac.nz:800/Department/EMUnit.html
} http://www.otago.ac.nz/anatomy/emunit/
}
}
}
}
}
}

===========================================================================
Mr. Ron Doole e-mail ron.doole-at-materials.ox.ac.uk
Department of Materials, phone +44 (0) 1865 273701
University of Oxford, fax +44 (0) 1865 283333
Parks Road.
Oxford. OX1 3PH. UK.
============================================================================






From daemon Thu Jul 27 08:49:36 2000



From: Jonathan Barnard :      Jonathan.Barnard-at-angstrom.uu.se
Date: Thu, 27 Jul 2000 15:22:28 +0200
Subject: TEM: dislocations in Al-30Si

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Simon,
My advice would be to do a series of two-beam dark field images with the
Bragg reflection (g) chosen to optimise the g.b condition (b = Burgers
vector). First find out the dominant dislocation types in Al & Si alone (or
any other similar alloy) as a guide for the Burgers vectors you could
possibly expect. If you can, try a reflection which makes g.b non zero for
all dislocation types (giving contrast for all dislocations), and then
subsequently select reflections that give g.b=0 for each type of
dislocation (or g.b non-zero for one dislocation type).
This procedure will allow you to deduce population densities for each
dislocation type. If the dislocation density is too high i.e. you cannot
resolve individual dislocations, then try a weak beam dark field imaging
instead e.g g-3g (3g set to Bragg, g imaged). Contrast in these images come
from the more highly strained crystal regions close to the dislocation core
and one can narrow the contrast down to the order of nanometers.

A note on your definition of dislocation densities. You mention dividing by
an area. Does this mean you are calculating the areal density or a volume
density? The former is usually attributed to growing thin films, and most
of the dislocation emanate from the initial interface. In that case you if
you are looking at a cross-section, you will need to know the thickness,
whereas a plan-view sample doesn't. For volume densities again you will
need the thickness too. Areal densities are pretty meaningless in 3D.

Dislocation analysis is a fantastic area with a huge history, and I would
advise you take a look at two books. Firstly Hirsch, Howie, Nicholson,
Whelan & Pashley (Electron Microscopy of Thin Crystals), described as the
Bible of transmission electron microscopy, has several chapters related to
imperfect crystals, and the black and white photos are quite beautiful,
even just to look at. Any time invested with this book pays off big.
A more modern book is Williams & Carter (Transmission Electron Microscopy)
and is extremely helpful. Again a superb selection of pictures and diagrams
will help you with the theory and practice.
********************************************************
Dr Jonathan Barnard

Analytical Materials Physics
The Angstrom Laboratory, Uppsala University
P O Box 534, SE-751 21 Uppsala, Sweden
Phone: +46-(0)18-4716838 Fax: +46-(0)18-500131
Phone: Microscope room +46 18 471 6365
http://www.angstrom.uu.se/analytical/home.html
********************************************************




From daemon Thu Jul 27 09:14:34 2000



From: Philip Oshel :      peoshel-at-facstaff.wisc.edu
Date: Thu, 27 Jul 2000 09:07:22 -0500
Subject: Re: Polaroid Film Choices

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Whatever for? What would the environmental folks disagree about with
the 55 processing? The sulphite? That's sprayed on fruit at the
grocery store. The coater? I hate that myself, but it's no worse than
regular darkroom chemicals.

T 52 won't replace T 55, as it has no negative. I don't think T 53 or
T 54 do either, but I'm not sure. The T 55 negative is an *excellent*
negative (in spite of some folks' comments otherwise) with about 3X
the resolution of the T55 positive and 2 more zones of contrast. No
positive-only film or digital camera can equal it. If you have to get
rid of T 55, you're better off changing camera backs and using 4X5
negative sheet film. Good photo stores should have this, or the big
mail-order companies like Helix or Shutan (in the back of photography
mags).

Phil

} Howdy List,
}
} I need to stop using Polaroid T-55 p/n sheet film in my lab(good bye old
} friend). The enviormental guys got to us.
}
} The question is, what to replace it with? T-52, T-53 or T-54.
}
} We will be using it primarily in our old Cambridge 250, inverted
} metallograph, and some macro stand usage.
}
} Its use will be limited as our new JEOL SEM and inverted metallograph are
} going digital.
}
} Any suggestions?
}
}
} William T. Giles
} Sr. Electron Microscopist
} Met. Lab. Coordinator
} Henderson Technical Laboratory
} TIMET
} PO Box 2128 Henderson NV 89009
} Ph: (702)566-4436
} Fax: (702)564-9038
} E-mail: Bill.Giles-at-timet.com

--
}}}}}}}}}}}}}}}}}{{{{{{{{{{{{{{{{{
Philip Oshel
Supervisor, AMFSC and BBPIC
Dept. of Animal Health and Biomedical Sciences
University of Wisconsin
1656 Linden Drive
Madison, WI 53706-1581
voice: (608) 263-4162
fax: (608) 262-7420 (dept. fax)



From daemon Thu Jul 27 09:14:34 2000



From: Leona Cohen-Gould :      lcgould-at-mail.med.cornell.edu
Date: Thu, 27 Jul 2000 09:57:57 -0400
Subject: Re: Flourescent stains- Microscope slides

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}
}
} Where can I find prepared microscope slides that use flourescence? Or, where
} can I find information about how to prepare my own slides with flourescent
} stains? I would like such samples for research on 3D microscopy.
}
} Sincerely,
}
} Jennifer Delille
}
} jen-at-sl3d.com
**********************
Jennifer,
Its unclear what you wnat to do....Are you planning to work with biological
samples that have been labelled with fluorescent markers, or is this a
materials-related project?
For biological samples, "normal" glass slides are fine. You usually do
need to treat the slides in some way to get your samples (sections of
tissue) to stick well enough to survive the multiple steps involved in the
labelling process. The traditional method is to sub (coat) the slides with
a gelatin-chrom alum solution. You can also use slides that have been
treated by the manufacturer (silanized or the mysterious but useful "plus"
slides).

The fluorescent labelling protocols depend on what you wih to look at.
some are quite simple, such as the use of propidium iodide to label nuclei,
others are multi-step, antibody protocls. You can find descriptions of
these in the many good texts about immuncytochemistry.

If you have some materials-related project in mind, someone else will have
to address that, I'm atrictly a biologicals person.

Lee

Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175





From daemon Thu Jul 27 09:48:15 2000



From: jim :      jim-at-proscitech.com.au
Date: Fri, 28 Jul 2000 00:17:56 +1000
Subject: RE: SEM spot size & x-ray analysis

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Its some time since I've looked at Goldstein's book, but I am sure you have
missed a chapter. First, its important to note that in microanalyses the area
irradiated is always smaller than the area you are collecting X-rays from. Its
all related to the penetrating envelope, Monte Carlo patterns, kV used and
average atomic number of the area under the beam. Typically, using a small
spot, you may at 15kV collect X-rays from a 20 um radius and depths if your
specimen is biological and only from 3um if its moderately heavy in atomic
numbers.
A good analyst would standardize all parameters, but probe current is more
important than spot size. A dedicated microprobe is equipped with a
lightmicroscope which has two main functions: Adjusting the specimen height to
the correct position (taking advantage of the lightmicroscope's poor depths of
view (or field) and visualising the beam spot so this can be centered, so the
spot is were we think it is. The beam is visualised through the lightmicroscope
by using a fluorescent mineral (Willemite). Centering using deflection coils
and adjusting the beam (or spot) size is easy with that scope. Without an
attached lightmicroscope, a small intense beam could be measured later by the
burnmark left on a sensitive specimen. As an aside, geologists new to
microanalysis are generally appalled by the poor quality of the lighmicroscope.
They are rarely fond of learning to use atomic number contrast and to do their
lightmicroscopy before a probe session.
However, I wonder about the usefulness of the proposed exercise, because the
relative beam size is usually less important because X-ray resolution is much
less than beam size anyway. All of the above applies of course to SEMs/Probes
since in a TEM (thin sections) the penetrating envelope is chopped off and the
beam diameter plus a bit becomes the area analysed.
Don't rush off and buy a TEM with analytical facilities, they have other
disadvantages in microanalyses.
Hope this clarified something . .
I should also disclaim: ProSciTech supplies WDS/EDS standards.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Thursday, July 27, 2000 10:23 AM, Gary Gaugler [SMTP:gary-at-gaugler.com]
wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I've studied Goldstein's book to try and accurately compute
} the true spot size of a SEM beam based on the myriad of
} factors which influence it. At issue is how to determine the
} actual spot size of a beam, in microns, at the specimen.
}
} SEMs from various makers use relative numbers for spot
} size. These indicate the relative size of the beam from
} "really small" to "largest." "Really small" means low
} specimen current but high resolution and the converse
} for the large spot size. If one is doing x-ray analysis,
} the spot size may or may not be an issue.
}
} If the area being analyzed is large, the spot size is
} most likely not all that important. But for the analysis
} of small feature size and sub-micron semiconductor devices,
} true spot size is an issue. If the beam covers more than
} the area of interest, the x-ray data will not accurately
} reflect the composition of the intended material.
}
} Therefore, is there some basic limitation of the applicability
} of quantitative SEM x-ray analysis based on the size of
} the specimen? If so, what is this limit? Also, is there
} some way to accurately define the physical size of a
} beam at the specimen? If so, what is this method?
}
} Any thoughts on this?
}
} gary g.
}




From daemon Thu Jul 27 10:10:23 2000



From: michael shaffer :      mshaf-at-darkwing.uoregon.edu
Date: Thu, 27 Jul 2000 07:59:02 -0700
Subject: RE: SEM spot size & x-ray analysis

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Gary writes ...

} Therefore, is there some basic limitation of the applicability
} of quantitative SEM x-ray analysis based on the size of
} the specimen? If so, what is this limit? Also, is there
} some way to accurately define the physical size of a
} beam at the specimen? If so, what is this method?

The applicability for the spot size regarding x-ray analysis would
have as much to do with the energy of the beam, i.e., the interaction
volume for x-ray generation (not to mention the possibility of x-ray
fluorescence within the surrounding material.

The best method for determining the spot size for any beam parameter,
is to examine the y-modulated intensity for a "knife edge". For
example, a perfect orthoganal slope would indicate infinitely small
.. for anything else you would determine the 'x' distance at 80% and
20% of the 'y' intensity (although these numbers could be debated).

cheerios, =shAf= :o)

{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {}
Michael Shaffer, R.A. - mshaf-at-darkwing.uoregon.edu
Geological Science's Electron Probe Facility - University of Oregon
http://epmalab.uoregon.edu/





From daemon Thu Jul 27 10:18:03 2000



From: chris smith :      chris.smith-at-bbsrc.ac.uk
Date: Thu, 27 Jul 2000 16:06:01 +0100
Subject: TEM film

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Hi Arun & Listers,
Kodak SO-163 has a similar speed rating to AFGA Scientia 23D56 & is
available in the same size.
Chris Smith, IACR-Rothamsted,UK.



From daemon Thu Jul 27 10:48:18 2000



From: William Tivol :      tivol-at-wadsworth.org
Date: Thu, 27 Jul 2000 11:30:11 -0400
Subject: Re: SEM spot size & x-ray analysis

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Gary Gaugler wrote:

Dear Gary,


} I've studied Goldstein's book to try and accurately compute
} the true spot size of a SEM beam based on the myriad of
} factors which influence it. At issue is how to determine the
} actual spot size of a beam, in microns, at the specimen.
}

As others have already said, the relevant size is that
of the volume from which x-rays are generated, and that
is larger than the beam due to scattering within the specimen.


}
} SEMs from various makers use relative numbers for spot
} size. These indicate the relative size of the beam from
} "really small" to "largest." "Really small" means low
} specimen current but high resolution and the converse
} for the large spot size. If one is doing x-ray analysis,
} the spot size may or may not be an issue.
}

If the spot size is small compared to the distances
that the electrons will typically be scattered, then the x-ray
emission volume will be nearly independent of spot size,
but if the relevant scattering distance is small, then the spot
size will affect the spatial resolution of the analysis. Fur-
thermore, the emission volume will be smaller if the over-
voltage for the line of interest is low. This is because scat-
tering through large angles--which leads to large trans-
verse momentum and large effective spot size--results in
large transfer of kinetic energy, thus lower (or zero) cross
section for the production of the line of interest. So if you
use the k-lines and a relatively low voltage, the x-rays
will be emitted from a smaller volume than if you use l-
lines with the same voltage or k-lines with a higher vol-
tage.



} If the area being analyzed is large, the spot size is
} most likely not all that important. But for the analysis
} of small feature size and sub-micron semiconductor devices,
} true spot size is an issue. If the beam covers more than
} the area of interest, the x-ray data will not accurately
} reflect the composition of the intended material.
}

Yes, although knowing the characteristics of the
emission volume could allow you to deconvolute that
effect and get a better idea of the compositions of the
small areas of interest. That is, if you know the compo-
sition of one area and the apparent composition of an
adjacent area, you could use a Monte Carlo simulation to
compute the part of the apparent composition due to x-rays
generated within the first area. There are many such simu-
lation programs out there, and you want one which will
calculate not only the paths of the electrons, but also the
x-ray production. I do not know which of the simulation
programs will do this, but perhaps their authors will tell
you.


}
} Therefore, is there some basic limitation of the applicability
} of quantitative SEM x-ray analysis based on the size of
} the specimen? If so, what is this limit? Also, is there
} some way to accurately define the physical size of a
} beam at the specimen? If so, what is this method?
}

Yes. It depends on specimen composition and what is
of interest. Yes. Either simulation or doing an experiment
with a specimen that has well characterized features of known
size and composition. This specimen should be as similar to
what you're trying to measure as possible to assure that both
features of interest and matrix effects are well modelled. In
the case of semiconductor devices, perhaps the Ge stripes in
Si matrix of a MAGICAL test specimen could be used. (I
have no connection with MAGICAL except as a user.)
Yours,
Bill Tivol




From daemon Thu Jul 27 11:24:57 2000



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Thu, 27 Jul 2000 09:11:29 -0700
Subject: Re: SEM spot size & x-ray analysis (longish)

Contents Retrieved from Microscopy Listserver Archives
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Dear Gary,
Yes, there certainly is a size limit to a specimen or phase or particle in
the specimen to get accurate SEM x-ray analysis. I use the Small World's
Electron Flight Simulator to get a good estimate of the size of the electron
interaction volume in a given material. These are all Monte Carlo
mathematical simulations, since the SEM spot is not a spot, but a fuzzy disk
and the volume is a randomly generated approximation based on the factors
influencing the interaction of the electrons and material of the solid. What
is seldom mentioned is that the the x-rays generated by the primary
electrons can also spread laterally in the solid and these x-rays can spread
much further than the initial excitation volume and excite secondary x-rays
several microns away. These secondary x-ray effects vaary widely, dependant
on which elements are being excited and fluoresced.
There are two ways to test these effects. The first is to carefully scan the
SEM beam across a sharp interface between two materials, such as Ni plated
on polished Cu, and record the shape of the Ni and Cu line scans. The length
of the curve dropping between 100% Cu and 0% Cu will tell you the size of
the beam interaction volume in the solid (which is not the spot size, but
the size of the x-ray generation volume). The second is to drill a small
hole in a copper planchet and stick a tungsten aperture over the hole.
Slowly scan the beam over the Cu, W and Faraday cup and see where you can
see Cu and W x-rays. This gives you some idea of the beam spread and x-ray
scatter.
At 05:22 PM 7/26/00 -0700, you wrote:

} I've studied Goldstein's book to try and accurately compute
} the true spot size of a SEM beam based on the myriad of
} factors which influence it. At issue is how to determine the
} actual spot size of a beam, in microns, at the specimen.
}
} SEMs from various makers use relative numbers for spot
} size. These indicate the relative size of the beam from
} "really small" to "largest." "Really small" means low
} specimen current but high resolution and the converse
} for the large spot size. If one is doing x-ray analysis,
} the spot size may or may not be an issue.
}
} If the area being analyzed is large, the spot size is
} most likely not all that important. But for the analysis
} of small feature size and sub-micron semiconductor devices,
} true spot size is an issue. If the beam covers more than
} the area of interest, the x-ray data will not accurately
} reflect the composition of the intended material.
}
} Therefore, is there some basic limitation of the applicability
} of quantitative SEM x-ray analysis based on the size of
} the specimen? If so, what is this limit? Also, is there
} some way to accurately define the physical size of a
} beam at the specimen? If so, what is this method?
}
} Any thoughts on this?
}
} gary g.

Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca




From daemon Thu Jul 27 11:44:34 2000



From: Belluso elena :      belluso-at-dsmp.unito.it
Date: Thu, 27 Jul 2000 18:33:03 +0200 (MDT)
Subject: rotation angle between SAED and image in TEM

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Hello everyone,
I have a TEM Philips CM12 and one problem of this type of microscope is to
know all the rotation angles between the SAED pattern and the image in every
magnification. Surely it is possible to use a test specimen to determine
this angles, but may be there is someone had done this and he have this list
and he can help me.

Thank you everyone,
Elena Belluso



----------------------------------------------------
Elena BELLUSO
Dipartimento di Scienze Mineralogiche e Petrologiche
Via Valperga Caluso, 35
I-10125 TORINO - ITALIA
tel:(39) 011 670 7135 - fax: (39) 011 670 7128
e-mail: belluso-at-dsmp.unito.it
http://www.dsmp.unito.it
----------------------------------------------------




From daemon Thu Jul 27 11:50:09 2000



From: michael shaffer :      mshaf-at-darkwing.uoregon.edu
Date: Thu, 27 Jul 2000 09:39:16 -0700
Subject: SE: JEOL 840/63xx noisy roughing pump

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Our roughing pump which service our JSM6300 has began making a
rumbling noise which I might believe is bearings ... altho it could be
the pump's bearings or the motor's. Has anyone serviced this problem?

tia and cheerios, =shAf= :o)

{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {}
Michael Shaffer, R.A. - mshaf-at-darkwing.uoregon.edu
Geological Science's Electron Probe Facility - University of Oregon
http://epmalab.uoregon.edu/





From daemon Thu Jul 27 12:51:19 2000



From: Adriana Pinheiro Martinelli Rodriguez :      adriana-at-cena.usp.br
Date: Thu, 27 Jul 2000 14:41:49 -300
Subject: LM: in situ hybridization

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Dear list member:

Would someone direct me to websites and/or texts about in situ hybridization techniques? I'll be working with gene expression in shoot meristems from plant material (in vivo and in vitro).
For regular LM I use glutaraldehyde+paraformaldehyde as a fixative and Historesin (Leica) for embedding, but I don't think this method would be appropriate.
I'd appreciate some input.
Thank you very much in advance

Adriana



From daemon Thu Jul 27 13:34:47 2000



From: Rahbari, Ramin :      RAMIN.RAHBARI-at-WL.com
Date: Thu, 27 Jul 2000 14:23:09 -0400
Subject: Re: antibody

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Are you sure the endothelial cells you're after express PECAM1/CD31 in
appreciable amounts. I have found a great deal of disparity in CD31
staining in mouse tissue. I would suggest vWF or factor VIII or AP2 as
potential substitutes.

Ramin Rahbari
Pfizer Global Research & Development
Drug Safety Evaluation
2800 Plymouth Road
Ann Arbor, MI 48105
Voice (734) 622-3383
Fax (734) 622-5001
Ramin.Rahbari-at-WL.COM



-----Original Message-----
} From: Michael Reiner [mailto:Elektronenmikroskopie-at-web.de]
Sent: Wednesday, July 26, 2000 5:32 AM
To: Neelima Shah; Microscopy-at-sparc5.microscopy.com


Hello Neelima Shah!

Did you try PECAM-1/CD31 (rat) from Pharmingen? It must work well at least
using a pre-embedding protocol without permeabilization.

Greetings,
Michael Reiner

University of Cologne, Germany
Department of Anatomy I



} Anyone out there know of any antibody that would be specific for mouse
} endothelial cells?? We are trying to identify endothelial cells in tumors
} as well as cultured endothelial cells. I know a lot of them work for LM
} after various treatments. I seem to have had no luck so far staining at EM

} level. I would appreciate all the input.Thanks in advance.....
} Neelima Shah..............
} Biomedical Imaging Core Facility
} Uni of Pennsylvania
} Philadelphia, Pa.
}
}
}
}
} http://www.MED.upenn.edu/morphlab/
}
} è


_______________________________________________________________________
1.000.000 DM gewinnen - kostenlos tippen - http://millionenklick.web.de
IhrName-at-web.de, 8MB Speicher, Verschluesselung - http://freemail.web.de




From daemon Thu Jul 27 13:52:09 2000



From: Tamara Howard :      howard-at-cshl.org
Date: Thu, 27 Jul 2000 14:35:51 -0400 (EDT)
Subject: Re: LM: in situ hybridization

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have 2 review refs for mRNA in situ in plants:

Jackson, D.P. 1991. In situ hybridization in plants. In: Molecular Plant
Pathology - A Practical Approach. (sorry, I don't have the page numbers)

McFadden, G.I. 1995. In situ hybridization. Meth. Cell Biol. 49:165-183

Good luck!

Tamara Howard
CSHL

On Thu, 27 Jul 2000, Adriana Pinheiro Martinelli Rodriguez wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear list member:
}
} Would someone direct me to websites and/or texts about in situ hybridization techniques? I'll be working with gene expression in shoot meristems from plant material (in vivo and in vitro).
} For regular LM I use glutaraldehyde+paraformaldehyde as a fixative and Historesin (Leica) for embedding, but I don't think this method would be appropriate.
} I'd appreciate some input.
} Thank you very much in advance
}
} Adriana
}
}




From daemon Thu Jul 27 13:59:59 2000



From: Warren Davis :      warren.davis-at-path.utah.edu
Date: 27 Jul 2000 12:46:27 -0600
Subject: LM-Medical Systems Corp.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


I have recently inherited a great deal of equipment (stage warmers,
micro-incubators, perfusion chambers, etc) distributed by Medical Systems Corp. of Greenvale, NY. I've been told that they're still productive, yet I've been unable to locate any current contact information. Does anyone know whether they still exist and how I might be able to contact them?

Warren Davis
Cell Imaging Facility
School of Medicine
University of Utah
40 N. 2030 E.
Bldg. 585, Rm. 55
Salt Lake City, UT 84112
Ph: (801) 587-7964
email: warren.davis-at-path.utah.edu





From daemon Thu Jul 27 14:50:13 2000



From: ldm microscopy :      ldm2-at-risc4.numis.nwu.edu
Date: Thu, 27 Jul 2000 14:53:25 -0500 (CDT)
Subject: Postdoctoral Opening

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html
by ultra5.microscopy.com (8.9.1b+Sun/8.9.1) id OAA12567
for dist-Microscopy; Thu, 27 Jul 2000 14:44:55 -0500 (CDT)
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for {Microscopy-at-sparc5.microscopy.com} ; Thu, 27 Jul 2000 14:53:25 -0500 (CDT)


A postdoctoral position is open immediatesly for work on
Environmental Catalysts using UHV-TEM. The position will involve
looking at the the surface structure of a number of different oxides
as a function of gas treatments using our UHV instrument (see
http://www.numis.nwu.edu .) Experience in catalysis, UHV systems and
a strong background in TEM (not just a few courses) preferred. Send a
CV and letters of recommendation (or referees names) to

Laurence Marks
Department of Materials Science and Engineering
Northwestern University
ldm2-at-risc4.numis.nwu.edu

Email preferred over snail mail.







From daemon Thu Jul 27 15:14:28 2000



From: Evgenia Pekarskaya :      evgenia-at-caltech.edu
Date: Thu, 27 Jul 2000 13:01:28 -0700
Subject: Re: TEM dislocation density measurment in Al

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Simon,

What is frequently measured in TEM is scalar dislocation density (the
sign of dislocations
is not taken into account), even though this is not the only thing
that it is possible to measure (e.g. excessive dislocation density).

Concerning the scalar dislocation density (which is simply called
dislocation density), it is better to use such diffraction conditions
when there are several strong reflections present, then most of the
dislocations should be visible. I would recommend to use this
condition. However, as Witold Zielinski wrote, you can use two-beam
conditions but then you should take several images at different
conditions and analyze what is visible and what is not.
Alternatively, using two beam conditions a correction factor can be
introduced (see ref. 1 below).

It is also very important to know the thickness of the analyzed
area, you will need it to calculate dislocation density in your
material.

Another important consideration is rearrangement of dislocations due
to the stress relaxation after removal of the load and during
thinning due to the image force. Therefore, it is better to analyze
thicker area of the sample. You can calculate this force (ref 3) and
estimate the thickness of the sample where you should perform your
measurement for a particular material.

Total error of your measurements, which you can estimate (e.g. ref
10), will be around 20%.

I would recommend to look through this references. If you won't find
everything you need, you can contact me directly and I will fax you
some additional information.

1. Hirsch et al. 1965
Electron Microscopy of thin crystals

2. D.G. Brandon, Y. Komem
Metallography, 1970, v.3, 111

3. A. Seeger,
Handbuch der Physik, 1955, v. VII/1, 560.

4. J.W. Steeds, Roc. Roy. Soc., 1966, A292, p.343

5. J.E. Bailey, Phil. Mag. 1963, v. 8, p.223.

6. J.R. Hancock, Phil. Mag., 1968, 18, p. 1235

7. Baker T.N., ed. 1983
Yield, flow and fracture of polycrystals
London, Applied Science, 1983

8. Ecob R.C.,
J. Microscopy, 137, 1985, p. 3

9. Ivanov A.I.Y., Mezhennyi O., Ostrov A.E., Fomicheva E.I.
Zavodskaya laboratoriya, 53 (2), 1987, p.43 (this is in Russian, but
there is a copy of this paper in English).

10. Staker M.R. and Holt D.L
Acta metall. 20, 1972, p. 569

11. Finally, there is an excellent book in Russian, may be you can
find somebody to translate to you several pages from there.
Utevskii L.M. "Diffraction elecron microscopy in metallurgy", Moscow,
Metallrgiya, 1973.

Good luck,

Evgenia



} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

******************************************
Evgenia Pekarskaya
Materials Science
Mail Stop 138-78
California Institute of Technology
Pasadena, CA, 91125, USA
tel: 1-626-3953571
fax: 1-626-7956132



From daemon Thu Jul 27 15:21:42 2000



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Thu, 27 Jul 2000 10:10:40 -1000 (HST)
Subject: M&M2000 Exhibitor Demonstrations

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


For those of you attending Microscopy & Microanalysis 2000 in Philadelphia
next month-

Once again, MSA's Education Committee is organizing mini-seminars or
tutorial demonstrations known as "Exhibitor Demonstrations". These
sessions are conducted by Exhibitors in their booths on Tuesday evening
from 5:00 to 6:00 pm (or some fraction thereof). To attend one of these
demonstrations you must sign up at MSA's Education Booth, now part of the
MSA "Mega-Booth", before Tuesday noon. You will be issued a ticket that
will allow you back into the Exhibit Hall after it is closed to regular
attendees that evening. A list of titles and a short description of each
demonstration will be available at the Education Booth. While you are
there, see what else the Education Committee has to offer! Below is a
current list of participating Exhibitors:

Advanced Microscopy Techniques
Digital Instruments/Veeco Metrology Group
E.A. Fischione Instruments
EDAX
Electron Microscopy Sciences
Energy Beam Sciences
Evex Analytical
FEI Company
Illumea Corp.
KS Electron Technologies
LEO Electron Microscopes
Microbiology International/Syncroscopy
Micro Photonics
Microscopy/Marketing & Education
Motic
NORAN Instruments
Photon Technology International
SELA USA
South Bay Technology
Ted Pella
TSL

See you in Philly-

Tina

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************





From daemon Thu Jul 27 16:26:50 2000



From: Gary Gaugler :      gary-at-gaugler.com
Date: Thu, 27 Jul 2000 14:01:59 -0700
Subject: Re:SEM spot size & x-ray analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 05:59 AM 7/27/00, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Yes, it is covered but does not seem to lead one to a finite conclusion.
The big variable is electron optics. My current FESEM has a flat pole
piece. My LaB6 SEM has a 60 degree conical lens. Other units
have different angles. The lens design must play some major portion
of the whole operation of getting from the emitter to the specimen. It
is a tight system.


} Don Chernoff has available (http://www.small-world.net/efs.htm) a program
} which
} models the analysis volume. A similar but less flexable program came with
} my
} (former) Kevex EDS in the mid 80s. I have not tried the EFS software, nor
} have
} I any ties to small-world.
}
} Woody White
} McDermott Technology, Inc.

Thanks for the URL. I will check this link out.

gary g.




From daemon Thu Jul 27 17:17:19 2000



From: White, Woody N :      Woody.N.White-at-mcdermott.com
Date: Thu, 27 Jul 2000 17:50:00 -0500
Subject: Re[2]:SEM spot size & x-ray analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Since so many variables come into play, it is not surprising that details
are a bit vague in the book. Haven't referred to it in quite a while and
I forget the exact wording...
Taming the variables is where the statistical software is handy.

I can't imagine differing lens construction making a difference in the
analysis volume other than a conical lens permitting higher tilt angles
for certain specimens which will cause the volume to skew along the
surface (the incident beam angle variable). Have I missed something?

Since the beam diameter is typically MUCH smaller than the analysis
volume, variations in its size will not significantly affect the
x-ray analysis resolution. One exception to this is WDS analysis.
Sometimes HUGE beam currents are desired. This can lead to a(fat)beam
that will limit x-ray resolution. Generally speaking, if you can see a
crisp SE image at the desired magnification, the lens configuration
and beam diameter are not part of the analysis volume equation.
At this point you may or may not have enough beam current to achieve
the desired count rate.
Rule of thumb: Only if you must increase the beam current/spot size
(to raise the count rate) until the SE image is no longer well resolved
is it time consider beam size affecting the outcome.

Woody
-------------------------------------------------------------
At 05:59 AM 7/27/00, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Yes, it is covered but does not seem to lead one to a finite conclusion.
The big variable is electron optics. My current FESEM has a flat pole
piece. My LaB6 SEM has a 60 degree conical lens. Other units
have different angles. The lens design must play some major portion
of the whole operation of getting from the emitter to the specimen. It
is a tight system.


} Don Chernoff has available (http://www.small-world.net/efs.htm) a program
} which
} models the analysis volume. A similar but less flexable program came with
} my
} (former) Kevex EDS in the mid 80s. I have not tried the EFS software, nor
} have
} I any ties to small-world.
}
} Woody White
} McDermott Technology, Inc.

Thanks for the URL. I will check this link out.

gary g.



From daemon Thu Jul 27 17:36:45 2000



From: waltew82-at-eng.uab.edu
Date: Thu, 27 Jul 2000 17:21:49 -0500
Subject: double image of TEM filament

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello I am observing a double image from my TEM filament. The microscope has
been aligned over and over. If anyone has seen this please help
Thanks
Wilbur



From daemon Fri Jul 28 19:40:30 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Fri, 28 Jul 2000 08:02:32 -0500
Subject: Administrivia: Surplus Equipment Forum

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Colleagues....

A few days ago I reminded everyone about the abuses that
were starting to creep into the Listserver Re: Selling of Items.

At that time I promised to create something which would address
that issue. Well I've bitten the bullet and got the prototype completed
and running.

There now exists a WWW site where any individual can post
an electronic advertisement to dispose of SURPLUS equipment (ie not
new instrument sales). This site is open to all individuals,
organizations and vendors. The URL is:

http://www.msa.microscopy.com/SurplusEquipment/SurplusLisings.html

This is not intended to take the place of the Vendor News
Forum which has been running for over a year at:

http://www.msa.microscopy.com/News/NewsListings.html


All postings to the Surplus Equipment Forum are reviewed (by guess who)
to make
sure they are not junk/spam/bogus postings but not reviewed for accuracy.

There are no charges to use this Forum, however, I am suggesting
an honor system. If you use the Forum to dispose of excess equipment
and you receive more than shipping costs, then you should consider
voluntarily donating an appropriate amount to the Society to help
defray operating costs of this site. I will not monitor this aspect
as I have neither the time nor the inclination to do so.

Remember, if you have items that you are giving away for at most nominal
shipping costs, then by all means continue to use the Microscopy
Listserver. It was created with that spirit in mind and I wish
that tradition to continue.

This is clearly an experiment. There will of course be some problems
and growing pains and if it doesn't work then I've only lost a few hours
of time. However, I expect that it could be reasonably successful
and fill a need in our community. So have at it.

Cheers....

Nestor
Your Friendly Neighborhood SysOp..

.. as he heads off to the sun set to get dinner and a beer....







From daemon Fri Jul 28 19:44:00 2000



From: Ann R Soleman :      wellness4all-at-juno.com
Date: Fri, 28 Jul 2000 09:38:55 -0400
Subject: Soft Touch II grey tips #00593, Cover slides

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Colleagues

Does some one know of a source for:
Soft Touch II grey tips Catalog Number #00593, manufactured
by the Boehringer Mannheim Company of Indianapolis, IN
(for use with Soft Touch II Lancet Device) in or near the
Washington, D. C. -- Northern Virginia -- Maryland area?

They are still being manufactured, as per our conversation with the
manufacturer (last month - June) even though it is an older item..
However the manufacturer only ships to wholesalers. Many suppliers
no longer carries them. . .

OR perhaps someone in the DC area has switched to a newer Lancet
device and might still have some grey tips in stock..

Also need a local source for frosted slides 25x75 mm x 1mm thick.

OR would there be a source of help near Atlanta, GA? I'll be in Atlanta
in
conference (July 30-August 4). The need is immediate -- I am traveling
with
an Olympus CH30 phase contrast microscope, performing live blood cell
demonstrations and these are no longer available from our own supplier.
Any information would be greatly appreciated. Thank you in advance...

Gratefully,
Ann Soleman, BA, BS, ThM.
Certified Nutritional Microscopist

Wellness-4-All
Post Office Box 250
Oakton, VA 22124 USA
www.wellness4all-at-eyionline.com + Visit My Web Site + passcode:
wellness4all
E-mail: wellness4all-at-juno.com
E-mail: RASoleman-at-aol.com
E-mail: anns-at-eyionline.com
Voice Mail: 1-877-881-2593
Electronic Fax: 1-877-881-2593
Phone 1-703-591-1232
________________________________________________________________
YOU'RE PAYING TOO MUCH FOR THE INTERNET!
Juno now offers FREE Internet Access!
Try it today - there's no risk! For your FREE software, visit:
http://dl.www.juno.com/get/tagj.



From daemon Fri Jul 28 19:44:01 2000



From: E. J. McKenzie :      elizm-at-pdx.edu
Date: Fri, 28 Jul 2000 09:30:59 -0700
Subject: EDS resolution

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi everyone,

we are having serious problems with the resolution of our EDS detector - the
peak height-width ratio is terrible, around 450 counts. Consequently, our
peaks look more like rolling hills and one peak blurs into another. I have
callibrated the machine using an Al-Cu standard, checked that the detector
is kept cool, sample is tilted at 30 degrees, etc etc. I also got another
microscopist to come and have a look to check my methods and he also
concluded there was a serious problem.

Is there anything else I could try before I phone the Noran technicians and
pay them a lot of $$$ to fix/replace the detector?

regards
Liz McKenzie
--------------------------------------------------
Geomicrobiology and Electron Microscopy Laboratory
Room S9 Cramer Hall
1721 SW Broadway
Portland State University
Portland
OR97201

ph:503 725 3362
fax:503 725 3025




From daemon Fri Jul 28 19:45:02 2000



From: Gary Gaugler :      gary-at-gaugler.com
Date: Thu, 27 Jul 2000 20:29:01 -0700
Subject: Re[2]:SEM spot size & x-ray analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 03:50 PM 7/27/00, you wrote:
} Since so many variables come into play, it is not surprising that details
} are a bit vague in the book. Haven't referred to it in quite a while and
} I forget the exact wording...
} Taming the variables is where the statistical software is handy.
}
} I can't imagine differing lens construction making a difference in the
} analysis volume other than a conical lens permitting higher tilt angles
} for certain specimens which will cause the volume to skew along the
} surface (the incident beam angle variable). Have I missed something?
}
} Since the beam diameter is typically MUCH smaller than the analysis
} volume, variations in its size will not significantly affect the
} x-ray analysis resolution. One exception to this is WDS analysis.
} Sometimes HUGE beam currents are desired. This can lead to a(fat)beam
} that will limit x-ray resolution. Generally speaking, if you can see a
} crisp SE image at the desired magnification, the lens configuration
} and beam diameter are not part of the analysis volume equation.
} At this point you may or may not have enough beam current to achieve
} the desired count rate.
} Rule of thumb: Only if you must increase the beam current/spot size
} (to raise the count rate) until the SE image is no longer well resolved
} is it time consider beam size affecting the outcome.
}
} Woody

The link below shows what I am trying to analyze.

http://photoweb.net/ICxsect.jpg

I know that PSG, Al and poly si are correctly marked. The poly
gate is what is confusing me. Its contrast is like the surrounding
oxide while its interior is like the poly layer....or it could be metal
over poly.

Based on these rather small feature sizes, will quantitative x-ray
analysis produce viable results? If I probe the small central
area of the gate, will I get the results of that area or the results
of it and the surrounding material?

gary g.




From daemon Fri Jul 28 19:51:21 2000



From: Gary Gaugler :      gary-at-gaugler.com
Date: Thu, 27 Jul 2000 16:30:31 -0700
Subject: Re: SE: JEOL 840/63xx noisy roughing pump

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



when the pumps start to go bad, it seems to be preceded by
leaking at the motor-pump interface. The unit goes downhill
from there.

If you don't have any leaking oil, does the pump sound like
knocking? I've heard this and found that it was due to an
air leak. If the exhaust close off control on the pump is
closed, and the knocking persists, it means there is an air
leak somewhere. You should see this in poorer vacuum readings.

Have you ever changed the oil?

gary g.

At 09:39 AM 7/27/00, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From daemon Fri Jul 28 20:07:47 2000



From: Steven Slap :      SSlap-at-ebsciences.com
Date: Fri, 28 Jul 2000 09:51:17 -0400
Subject: Nitrogen developer agitation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear fellow microscopists,

We recommend nitrogen-burst agitation for one second out of every three
seconds. This timing is built into our Autoprocessor. We will have a
small model system in our booth at M & M in Philadelphia.

Best regards,
Steven Slap

******************************
Energy Beam Sciences, Inc.
The Laboratory Microwave Company
http://www.ebsciences.com
Adding Brilliance to Your Vision
******************************




From daemon Fri Jul 28 20:09:36 2000



From: Belluso elena :      belluso-at-dsmp.unito.it
Date: Fri, 28 Jul 2000 20:00:03 -0500
Subject: Need mineralogical samples for TEM studies

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thank you very much for mineralogical samples I have received from Martin J.
Roe.

Regards,
Elena



----------------------------------------------------
Elena BELLUSO
Dipartimento di Scienze Mineralogiche e Petrologiche
Via Valperga Caluso, 35
I-10125 TORINO - ITALIA
tel:(39) 011 670 7135 - fax: (39) 011 670 7128
e-mail: belluso-at-dsmp.unito.it
http://www.dsmp.unito.it
----------------------------------------------------





From daemon Fri Jul 28 20:23:55 2000



From: Angela Klaus :      avklaus-at-amnh.org
Date: Fri, 28 Jul 2000 18:01:51 -0400
Subject: P-T Laboratory Assitant Position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Part-time Laboratory Assistant Position - 20 hours/week

The American Museum of Natural History is seeking a Part-Time Laboratory
Assistant for its state-of-the-art core imaging and image processing
facility. The Interdepartmental Laboratory houses an Hitachi Cold Field
Emission Scanning Electron Microscope/PGT EDS, a Zeiss Laser Scanning
Confocal Microscope, and image processing/high quality output resources.

Major responsibilities include assisting scientific staff with imaging and
x-ray microanalysis projects, and training users in independent operation
of microscopes and computers. Other laboratory duties include minor
equipment maintenance, occasional specimen preparation, and other general
lab-related tasks.

A Bachelors degree in biology is preferred, along with some knowledge of or
interest in other scientific disciplines such as geology or materials
science. Candidates who are currently enrolled in a scientific
undergraduate or graduate program will also be considered. The position
requires some knowledge of SEM and strong computer skills. Other
qualifications include willingness to learn, flexibility, and excellent
organizational and interpersonal skills.

Hourly rate is commensurate with experience. Interested candidates can
contact me directly.









---------------------------------------------
Angela V. Klaus

Director - Interdepartmental Laboratories
American Museum of Natural History
Central Park West at 79th Street
New York, NY 10024-5192 USA

Email: avklaus-at-amnh.org
Tel: 212-769-5977
Fax: 212-496-3480
---------------------------------------------



From daemon Fri Jul 28 20:58:16 2000



From: Connolly, Brett :      brett_connolly-at-merck.com
Date: Fri, 28 Jul 2000 11:11:33 -0400
Subject: Monitor for imaging

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I'm seeking recommendations for 20-21 color monitors to use for histological
image analysis. Important parameters are high resolution and color
reproduction, refresh rates of at least 85Hz and , of course, reliability.
I've heard some good things about Viewsonics and the Sony GDM-500PS. Are
there any I should avoid?

Thanks,
Brett


Brett M. Connolly, Ph.D.
Merck Research Laboratories
Department of Pharmacology
WP26A-3000
PO Box 4
West Point, PA 19486
Ph. 215-652-2501
FAX 215-652-2075
e-mail: brett_connolly-at-merck.com




From daemon Fri Jul 28 21:14:32 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Fri, 28 Jul 2000 21:04:54 -0500
Subject: Administrivia: Nestor Can't Spell & Router Outage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Oops... I can't spell correctly. The Surplus Equipment URL is:

http://www.msa.microscopy.com/SurplusEquipment/SurplusListings.html

(I left off the "t" in Listings)...

Also one of the main routers in Chicago went down on Thursday Evening
so there was no connectivity to the Server for the last 24 hours..

Oh well....

Nestor
Your Friendly Neighborhood SysOp ... or is is OpSys????





From daemon Fri Jul 28 21:43:29 2000



From: Gregory Mulhollan :      gregmulh-at-swbell.net
Date: Fri, 28 Jul 2000 21:39:59 -0500
Subject: ISI 100B Manual needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Microsope Folks,
I am going to have the fun of doing a minor rebuild on an old ISI 100B SEM.
Alas, the manual seems to not have arrived with this old beast and I am in
need of pointers to where I can obtain a copy. Thanks in advance!

Greg Mulhollan

Gregory Mulhollan
saxeT software & surface science
1808 B Cinnamon Path
Austin, TX 78704



From daemon Fri Jul 28 22:36:02 2000



From: Fauzi Mohd Som :      fauzi-at-lgm.gov.my
Date: Sat, 29 Jul 2000 11:24:35 +0800
Subject: Help on EDS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I just got this Oxford EDS Link QX2000 attached to JEOL JSM 5300. I wonder
if someone can enlighten me on way the Gross Integral and Net Integral are
calculated from the EDS Link QX2000 and also what is that STRB?

TQ.





From daemon Fri Jul 28 22:58:29 2000



From: Milo Kral :      m.kral-at-mech.canterbury.ac.nz
Date: Sat, 29 Jul 2000 15:47:54 +1200
Subject: Re: rotation angle between SAED and image in TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,
The rotation calibration is described in Edington, using MoO3
samples. If you have any kind of sample for which a crystallographic
direction is unambiguously known with respect to some linear feature
of the sample then you can use that too. It is not hard to do this
calibration. Just make double exposures (SAED and BF image) at each
magnification for each camera length you are interested in. I think
you can use a short cut: once the entire rotation calibration is done
for a single camera length, then you need only do one magnification
at any other camera length to get the whole thing - on the Philips
microscopes I've used in the past, diffraction patterns do not rotate
with change in camera length.

Milo


} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From daemon Sat Jul 29 09:58:39 2000



From: Harrison :      tuttle-at-home.com
Date: Sat, 29 Jul 2000 07:45:06 -0700
Subject: Re: double image of TEM filament

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi, It's likely caused by EMI, but could also be caused by a ground loop, or a
noisy power supply.

Regards, Dave Harrison



On 27 Jul 00, at 17:21, waltew82-at-eng.uab.edu wrote:


} Hello I am observing a double image from my TEM filament. The microscope has
} been aligned over and over. If anyone has seen this please help Thanks Wilbur
}





From daemon Sat Jul 29 15:33:20 2000



From: Gary Gaugler :      gary-at-gaugler.com
Date: Sat, 29 Jul 2000 13:23:35 -0700
Subject: Hummer VII coating thickness

Contents Retrieved from Microscopy Listserver Archives
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By chance, has anyone run any calibration coatings
with the Hummer VII using an Au/Pd target? The
coating thickness is dependent on the thickness
setting but also on the nm/minute rate. While the
system is not meant to be absolute, it does provide
a general idea of thickness. The question is
whether there is one or more rate settings which
have corresponding tables or relationships to end-point
thickness settings? I am running at 80mTorr.

thanks,
gary g.




From daemon Sat Jul 29 22:47:14 2000



From: Bruce Brinson :      brinson-at-cnst.rice.edu
Date: Sat, 29 Jul 2000 22:35:15 -0500
Subject: Re: Monitor for imaging

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Brett,
I personally have a PS 790 & it's predecessor (both 19" View Sonic). I am
quite happy with them. Max. refresh rates are 90hz at 1280x1024 & 76Hz at
1600x1200.... a no $ interest disclaimer applies here.
The price of large monitors has dropped significantly which puts good
monitors in the price range of not so good units.
When looking at large monitors one thing to be weary of is a spatial non
linearity across the screen (distortion). Unless your trying to make
measurements on the screen with a hand ruler this is generally not noticeable.
The larger the monitor, the more difficult this is to control. It is not
uncommon to find small magnets glued to the CRT. This is obviously not an
adjustable parameter. An easy way to look for/qualify the problem is to look to
see if a round object in the middle is round or oval by the edge. This can vary
with individual monitors. I have an expensive 19" (not VS) that has this problem
to a greater extent than my 2 VS monitors in which it exist just a bit. Actually
I hadn't even checked them 'til just now.
The other thing I have run it to (and not recently) is color or brightness
non-uniformity across the screen. Again this is often not noticed in normal
computer applications. Try putting up a white screen & working with the
brightness & contrast to check for this problem.

good luck,
Bruce Brinson
Rice U.


"Connolly, Brett" wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} I'm seeking recommendations for 20-21 color monitors to use for histological
} image analysis. Important parameters are high resolution and color
} reproduction, refresh rates of at least 85Hz and , of course, reliability.
} I've heard some good things about Viewsonics and the Sony GDM-500PS. Are
} there any I should avoid?
}
} Thanks,
} Brett
}
} Brett M. Connolly, Ph.D.
} Merck Research Laboratories
} Department of Pharmacology
} WP26A-3000
} PO Box 4
} West Point, PA 19486
} Ph. 215-652-2501
} FAX 215-652-2075
} e-mail: brett_connolly-at-merck.com
}




From daemon Sun Jul 30 01:36:13 2000



From: Peter Jordan :      emsi-at-pe.net
Date: Sat, 29 Jul 2000 23:17:02 -0700
Subject: Re: double image of TEM filament

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Have you tried to change the filament? That might take care of your problem.
Peter Jordan

"waltew82-at-eng.uab.edu"-at-sparc5.microscopy.com wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hello I am observing a double image from my TEM filament. The microscope has
} been aligned over and over. If anyone has seen this please help
} Thanks
} Wilbur




From daemon Sun Jul 30 15:36:03 2000



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Mon, 31 Jul 2000 08:21:54 GMT+1200
Subject: Re: Administrivia: Surplus Equipment Forum

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Nestor


}
} There now exists a WWW site where any individual can post
} an electronic advertisement to dispose of SURPLUS equipment (ie not
} new instrument sales). This site is open to all individuals,
} organizations and vendors. The URL is:
}

}
} This is clearly an experiment. There will of course be some problems
} and growing pains and if it doesn't work then I've only lost a few hours
} of time. However, I expect that it could be reasonably successful
} and fill a need in our community. So have at it.
}

This is a wonderful thing, I'm sure it will work and will be much
appreciated.

Will it be appropriate to post queries from those seeking items also,
such as me in my ceaseless quest for 840 accessories?

cheers (at least three)

rtch


Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand



From daemon Sun Jul 30 16:44:05 2000



From: wft03-at-health.state.ny.us
Date: Sun, 30 Jul 2000 17:34:47 -0400
Subject: Re: EDS resolution

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html





Dear Liz,

we are having serious problems with the resolution of our EDS detector -
the
peak height-width ratio is terrible, around 450 counts. Consequently, our
peaks look more like rolling hills and one peak blurs into another. I have
callibrated the machine using an Al-Cu standard,

You do not say what your total count rate is or whether it is the same
as it used to be for
the standard you are using. I assume that both count rate and dead time
are what they should be.
I also assume that the FWHM of the Mn k-alpha peak is } 150 eV (regardless
of height).

checked that the detector
is kept cool, sample is tilted at 30 degrees, etc etc. I also got another
microscopist to come and have a look to check my methods and he also
concluded there was a serious problem.

When this happened to us, it turned out that the vacuum had
deteriorated. After having sent
the detector back for reconditioning, we designed and installed a valve
which could be attached to
the EM column pumping system. This enabled us to warm up and pump out the
detector ourselves.
After about 15 years, however, this no longer worked, and we had to send
the detector for a pro-
fessional cleaning.

Is there anything else I could try before I phone the Noran technicians and
pay them a lot of $$$ to fix/replace the detector?

Get estimates for the various repair options. We decided to have Doug
Connors of TNAS
clean and overhaul our unit, since his price for that service was the
cheapest. After the bakeout and
pumpdown he performed, the resolution had returned to 147 eV (from ~200 eV
when we sent it in).
His charges for other services--which we hoped would not be needed--were
comparable to Noran's
and others'. We have no affiliation with Doug or TNAS except as customers.
Good luck.
Yours,
Bill Tivol





From daemon Sun Jul 30 16:44:05 2000



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Mon, 31 Jul 2000 09:33:06 GMT+1200
Subject: Re: SE: JEOL 840/63xx noisy roughing pump

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


}
}
} Our roughing pump which service our JSM6300 has began making a
} rumbling noise which I might believe is bearings ... altho it could be
} the pump's bearings or the motor's. Has anyone serviced this problem?
}

I guess that this isn't your problem, but a couple of times I've had
alarming sharp knocking noises from my belt-drive JEOL pumps, which
have been completely cured with a few squirts of drive-belt dressing
compound from an aerosol can.
Seems to be some sort of sticky tacky lubricant, works well for me.

cheers

rtch


Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand



From daemon Sun Jul 30 17:01:24 2000



From: wft03-at-health.state.ny.us
Date: Sun, 30 Jul 2000 17:52:52 -0400
Subject: Re: Re[2]:SEM spot size & x-ray analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html





Dear Gary,

The link below shows what I am trying to analyze.

http://photoweb.net/ICxsect.jpg

From looking at the image, I'd estimate that the features you need to
analyse
are about 1/2 micrometer for the body of the gate, and 100 nm for the
surrounding
material. For sufficiently low voltage and a sufficiently small beam you
might have
an emission volume small enough to obtain an analysis from the body. It's
less likely
that a meaningful analysis can be obtained just from the surrounding layer.
However,
you might be able to measure apparent elemental concentrations as a
function of po-
sition and calculate the concentrations in the layer with the assumption
that both body
and layer have uniform compositions--this is not a trivial assumption.

I know that PSG, Al and poly si are correctly marked. The poly
gate is what is confusing me. Its contrast is like the surrounding
oxide while its interior is like the poly layer....or it could be metal
over poly.

Here may be a problem. In order to reduce the size of the emission
volume, you
need to lower the voltage; however, if the voltage is too small, there
might not be a enough
overvoltage to obtain sufficient yield of a suitable x-ray line from the
elements in the layer.

Based on these rather small feature sizes, will quantitative x-ray
analysis produce viable results? If I probe the small central
area of the gate, will I get the results of that area or the results
of it and the surrounding material?

Could be. Probably just from the body with the right exsperimental
parameters.
Good luck.
Yours,
Bill Tivol





From daemon Sun Jul 30 23:06:11 2000



From: Rosemary White :      Rosemary.White-at-pi.csiro.au
Date: Sun, 30 Jul 2000 22:49:21 -0500
Subject: Re: LM: in situ hybridization

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Adriana,

You can do in situ hybridisation on plant material embedded in wax,
LRWhite, LRGold, BMM (butyl methyl methacrylate) and probably historesin.
I haven't looked for references on in situs in non-embedded material, but
there is no reason why you couldn't do this in the same way people do
antibody labelling of plant material for immunofluorescence. I've seen
bacteria processed for in situs without resin embedding.

Good review article: McFadden (1989) Cell Biol Int Rep 13:3-21 covers use
of wax and resin for in situs.

Quite a few people still use FAA as a fixative, which is OK for tissue work
but makes my cell biologist hair stand on end! However, you usually need
to do protease digestion if you fix with aldehydes as the probes have a
hard time getting in through a highly cross-linked protein matrix.

If you fix in 4% paraformaldehyde and embed in BMM, you can do both
antibody labelling and in situs on the same material. See Kronenberger et
al. (1993) Cell Biology International 17:1013-1021 for BMM embedding
protocol for in situs.

cheers,
Rosemary

}
} Dear list member:
}
} Would someone direct me to websites and/or texts about in situ
} hybridization techniques? I'll be working with gene expression in shoot
} meristems from plant material (in vivo and in vitro).
} For regular LM I use glutaraldehyde+paraformaldehyde as a fixative and
} Historesin (Leica) for embedding, but I don't think this method would be
} appropriate.
} I'd appreciate some input.
} Thank you very much in advance
}
} Adriana


Rosemary White
Microscopy Centre
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia

phone 61-2-6246 5475
fax 61-2-6246 5000
email r.white-at-pi.csiro.au





From daemon Mon Jul 31 02:26:54 2000



From: Andrew Chuvilin :      dusha-at-catalysis.nsk.su
Date: Mon, 31 Jul 2000 14:13:09 +0600
Subject: Vendors for CCD camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We continue the attempts to get a CCD camera for our JEM 2010.
Could I ask vendors to contact me off-list?

TIA

Dr. A.Chuvilin
Institute of Catalysis
av. Lavrentieva 5
Novosibirsk
Russia



From daemon Mon Jul 31 02:41:46 2000



From: Ron Doole :      ron.doole-at-materials.oxford.ac.uk
Date: Mon, 31 Jul 2000 08:33:29 +0100 (BST)
Subject: Re: Help on EDS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Dear Fauzi Mohd Som,

The Gross Integral and NetIntegral values on Oxford Instruments (ex
Link) EDS systems refer to the total number of counts in a set energy
window (Gross Integrals) and the number of counts in the energy window
after subtracting the background (Net Integrals). The background is
calculated by drawing a line between the top of the first window channel
and the top of the last window channel, all the area under that line is
assumed to be the background.

STRB is the zero energy strobe.

Have fun with your QX2000,

Ron

On Sat, 29 Jul 2000, Fauzi Mohd Som wrote:

} I just got this Oxford EDS Link QX2000 attached to JEOL JSM 5300. I wonder
} if someone can enlighten me on way the Gross Integral and Net Integral are
} calculated from the EDS Link QX2000 and also what is that STRB?
}
} TQ.
}
}
}
}

===========================================================================
Mr. Ron Doole e-mail ron.doole-at-materials.ox.ac.uk
Department of Materials, phone +44 (0) 1865 273701
University of Oxford, fax +44 (0) 1865 283333
Parks Road.
Oxford. OX1 3PH. UK.
============================================================================




From daemon Mon Jul 31 05:50:38 2000



From: Tim E. Harper :      tim-at-cmp-cientifica.com
Date: Mon, 31 Jul 2000 12:32:45 +0200
Subject: TEM analysis in Europe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

We are looking for TEM capability to run a few thin film cross section
samples. The films are ~100nm thick, & we need full crystallographic
analysis, as well as imaging of the top surface, interface with substrate
etc.

If anyone has these capabilities, preferably in Europe, can you contact me
off list?

Thanks

Tim

*****************************************************************
Tim E. Harper Managing Director
CMP Cientifica s.l.
Space & NanoTechnology Division
Phone +34 91 640 71 85 Fax +34 91 640 71 86
http://www.cmp-cientifica.com/





From daemon Mon Jul 31 07:20:01 2000



From: jim :      jim-at-proscitech.com.au
Date: Mon, 31 Jul 2000 22:09:48 +1000
Subject: FW: Re[2]:SEM spot size & x-ray analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Bill - you are an optimist. Very much doubt that anything close to 2 um
diameter or less can be truly quantitative (in SEM type instrument; within +/-
1% of reality). If you lower the kV you run out of oomph, if the specimen is
light (not needing much oomph, then the volume is even greater.
I like optistist because they persevere.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Monday, July 31, 2000 7:53 AM,
"wft03-at-health.state.ny.us"-at-sparc5.microscopy.com
[SMTP:"wft03-at-health.state.ny.us"-at-sparc5.microscopy.com] wrote:
}
}
} Dear Gary,
}
} The link below shows what I am trying to analyze.
}
} http://photoweb.net/ICxsect.jpg
}
} From looking at the image, I'd estimate that the features you need to
} analyse
} are about 1/2 micrometer for the body of the gate, and 100 nm for the
} surrounding
} material. For sufficiently low voltage and a sufficiently small beam you
} might have
} an emission volume small enough to obtain an analysis from the body. It's
} less likely
} that a meaningful analysis can be obtained just from the surrounding layer.
} However,
} you might be able to measure apparent elemental concentrations as a
} function of po-
} sition and calculate the concentrations in the layer with the assumption
} that both body
} and layer have uniform compositions--this is not a trivial assumption.
}
} I know that PSG, Al and poly si are correctly marked. The poly
} gate is what is confusing me. Its contrast is like the surrounding
} oxide while its interior is like the poly layer....or it could be metal
} over poly.
}
} Here may be a problem. In order to reduce the size of the emission
} volume, you
} need to lower the voltage; however, if the voltage is too small, there
} might not be a enough
} overvoltage to obtain sufficient yield of a suitable x-ray line from the
} elements in the layer.
}
} Based on these rather small feature sizes, will quantitative x-ray
} analysis produce viable results? If I probe the small central
} area of the gate, will I get the results of that area or the results
} of it and the surrounding material?
}
} Could be. Probably just from the body with the right exsperimental
} parameters.
} Good luck.
} Yours,
} Bill Tivol
}
}




From daemon Mon Jul 31 08:11:37 2000



From: Barr, Dennis B :      dennbarr-at-eastman.com
Date: Mon, 31 Jul 2000 08:58:47 -0400
Subject: RE: EDS resolution

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Broadened peaks can be caused by electromagnetic interference. We
experienced this problem several years ago when we decided to "tidy-up" the
maze of cables lying around on the floor. Without thinking, we neatly
coiled up the detector signal cable and hung it on a clip attached to the
back of the EDS monitor. Soon after, we began realizing that our peak width
had increased significantly. It was only after much pain and grief that we
realized what we had done. The intense electromagnetic field generated by
the flyback coil in the monitor was the culprit. As soon as we un-coiled
the cable the peak width returned to normal.

Look for sources of fields such as monitors, fluorescent lights, etc.
Moving them as little as a foot or so can make a big difference.

Dennis B. Barr (dennbarr-at-eastman.com)
Physical Chemistry Research Laboratory
Physical & Analytical Chemistry Research Division
Eastman Chemical Company
Kingsport, TN 37662-5150

B-150B, R-132E, (423) 229-2188


} -----Original Message-----
} From: E. J. McKenzie [SMTP:elizm-at-pdx.edu]
} Sent: Friday, July 28, 2000 12:31 PM
} To: microscopy-at-sparc5.microscopy.com
} Subject: EDS resolution
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi everyone,
}
} we are having serious problems with the resolution of our EDS detector -
} the
} peak height-width ratio is terrible, around 450 counts. Consequently, our
} peaks look more like rolling hills and one peak blurs into another. I have
} callibrated the machine using an Al-Cu standard, checked that the detector
} is kept cool, sample is tilted at 30 degrees, etc etc. I also got another
} microscopist to come and have a look to check my methods and he also
} concluded there was a serious problem.
}
} Is there anything else I could try before I phone the Noran technicians
} and
} pay them a lot of $$$ to fix/replace the detector?
}
} regards
} Liz McKenzie
} --------------------------------------------------
} Geomicrobiology and Electron Microscopy Laboratory
} Room S9 Cramer Hall
} 1721 SW Broadway
} Portland State University
} Portland
} OR97201
}
} ph:503 725 3362
} fax:503 725 3025
}



From daemon Mon Jul 31 08:52:03 2000



From: JHumenansky-at-phi.com
Date: Mon, 31 Jul 2000 08:38:35 -0500
Subject: Re: SE: JEOL 840/63xx noisy roughing pump

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Here are some possibilities for your noisy pump.

Shut down the instrument and remove the drive belt from the mechanical
pump/Motor. Restart the instrument to power the vacuum pump motor to
isolate which is faulty. Chances are that it is the vacuum pump and you
will hear the motor running quietly.
If it is the motor, replacement of the motor is required. If the noise is
coming from the vacuum pump you could change the oil, and that is worth a
try. If one or more of the bearings are defective they are easily replaced
but you will need a gasket kit.

If you are under a service contract that would be the best place to start.
Hope this helps

John Humenansky/Staff Scientist
Physical Electronics, Inc. (PHI)
6509 Flying Cloud Drive
Eden Prairie, MN 55344
952-828-6387




From daemon Mon Jul 31 08:54:36 2000



From: Jolanta Mesjasz-Przybylowicz :      mesjasz-at-nac.ac.za
Date: Mon, 31 Jul 2000 15:26:27 +0000
Subject: Elemental microanalysis in botany

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Colleagues,

I have been invited to present an overview talk on elemental microanalysis
in botany during the 7th International Conference on Nuclear Microprobe
Technology and Applications (ICNMTA'2000), Bordeaux 10-15 September 2000.
I would like to cover recent applications in research areas such as plant
physiology, agriculture and environmental pollution. Special emphasis will
be made on an update of nuclear microprobe applications, following the
research summarised during the Santa Fe conference in 1996 and published in
Nucl. Instr. Meth. B130 (1997) 335.
I also intend to report on problems solved using other microanalytical
techniques such as EDX, SIMS, LMMS, SXRFM and EELS.
I will greatly appreciate your cooperation in that matter as I do not want
to miss any related work in the overview. Please send me any information of
relevance, a reprint, fax copy or just an e-mail with the list of
publications.
Due to time restrictions I would be grateful for your fast reply.

Best regards

Jolanta Mesjasz-Przybylowicz

Dr Jolanta Mesjasz-Przybylowicz
Materials Research Group
National Accelerator Centre
PO Box 72
Faure 7131 South Africa
E-mail: mesjasz-at-srvnac3.nac.ac.za
Fax: +27-21-8433543
Phone: +27-21-8433820





From daemon Mon Jul 31 09:44:33 2000



From: Dunlap, Jonathan C. :      Jonathan.Dunlap-at-sylvania.com
Date: Mon, 31 Jul 2000 10:34:52 -0400
Subject: Nikon Coolpix 990

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Good Morning all,

We recently purchased a Nikon Coolpix 990 for documentation use, but
I am absolutely loving the resolution and capability of this camera. Does
anybody know where I can find an adapter to use it on my various scopes
throughout the lab? I know that they make some lenses for this camera, but
does anybody know of any third party adapters or methods besides some duct
tape and a steady hand holding to an eyepiece (just kidding...)?

Thanks for your help,
~Jonathan Dunlap




Jonathan Dunlap
Electronic Components and Materials
Analytical Laboratory Manager
Osram Sylvania Inc.
816 Lexington Avenue
Warren, PA 16365
Ph: 814-726-6991
Fax: 814-726-6956






From daemon Mon Jul 31 10:53:01 2000



From: William Tivol :      tivol-at-wadsworth.org
Date: Mon, 31 Jul 2000 11:34:30 -0400
Subject: Re: FW: Re[2]:SEM spot size & x-ray analysis

Contents Retrieved from Microscopy Listserver Archives
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jim wrote:

Dear Jim,

} Bill - you are an optimist. Very much doubt that anything close to 2 um
} diameter or less can be truly quantitative (in SEM type instrument; within +/-
} 1% of reality). If you lower the kV you run out of oomph, if the specimen is
} light (not needing much oomph, then the volume is even greater.
} I like optistist because they persevere.
}

Guilty. On the HVEM--much different, but with relatively large
beam size (~0.5 mu-m)--I was able to distinguish a feature ~0.25 mu-m
across. You're correct about quantitation.


} } I know that PSG, Al and poly si are correctly marked. The poly
} } gate is what is confusing me. Its contrast is like the surrounding
} } oxide while its interior is like the poly layer....or it could be metal
} } over poly.

To distinguish between oxide and a metal surrounding the poly,
all you would need is a qualitative analysis. That's what I am optimistic
about.
You're right about the greater volume for less dense materials.
Since the range of electrons is roughly the same in units of mg/cm^2
for all materials with the same number of protons as neutrons in the
nucleus, scattered electrons will travel further. The hope is that there
is enough oomph and small enough volume at a particular energy.
This could happen if there is a good low-energy (m or n) line for a
heavier element which is not either on top of a k or l line for a lighter
element and which is not buried in noise or too low a cross section.
Yours,
Bill Tivol




From daemon Mon Jul 31 10:58:39 2000



From: William Tivol :      tivol-at-wadsworth.org
Date: Mon, 31 Jul 2000 11:41:31 -0400 (EDT)
Subject: Re: rotation angle between SAED and image in TEM

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Belluso elena:

} I have a TEM Philips CM12 and one problem of this type of microscope is to
} know all the rotation angles between the SAED pattern and the image in every
} magnification. Surely it is possible to use a test specimen to determine
} this angles, but may be there is someone had done this and he have this list
} and he can help me.
}
Dear Elena,
The Mag-i-cal specimen has instructions for doing this. I am not
affiliated with this specimen or its vendor(s) except as a user.
Yours,
Bill Tivol



From daemon Mon Jul 31 11:02:36 2000



From: ERIC :      biology-at-ucla.edu
Date: Mon, 31 Jul 2000 08:52:42 -0700
Subject: CCD Digiatl Cameas for TEM

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To the wealth of knowledge on the Microscopy Listerver,


We are looking into purchasing a digital camera for our TEM... We have a
Philips 208S and do mostly diagnostic work here in the EM lab related to
kidney biopsies...

I have looked at two cameras.. The AMT 1K and the Gatan Dual view 1K.. They
both deliver acceptable images.. I was just wondering what kind of problems
have occurred with both cameras after that have been set up and in
operation? i.e. customer service? problems? breakdowns?

Thanks in Advance

Eric A. Rosen
UCLA Medical Center






From daemon Mon Jul 31 11:37:26 2000



From: Walck, Scott D. :      walck-at-ppg.com
Date: Mon, 31 Jul 2000 12:25:54 -0400
Subject: RE: rotation angle between SAED and image in TEM

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As was mentioned, the Mag-i-cal sample is really good for determining the
roation calibration. You can either buy or make a sample of MoO3 by heating
wire or foil in air until you get white smoke and waving a coated grid
around in the smoke.

You do have a 180 degree ambuguity in the diffraction pattern. To eliminate
that, go to a convergent beam mode and underfocus the pattern (decrease the
condenser lens strength) until you see the BF image in the disk. That will
correlate with both the image and the diffraction pattern.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)



} -----Original Message-----
} From: Belluso elena [mailto:belluso-at-dsmp.unito.it]
} Sent: Thursday, July 27, 2000 12:33 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: rotation angle between SAED and image in TEM
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListse} rver/FAQ.html
}
}
} --------------------------------------------------------------
} ---------.
}
}
} Hello everyone,
} I have a TEM Philips CM12 and one problem of this type of
} microscope is to
} know all the rotation angles between the SAED pattern and the
} image in every
} magnification. Surely it is possible to use a test specimen
} to determine
} this angles, but may be there is someone had done this and he
} have this list
} and he can help me.
}
} Thank you everyone,
} Elena Belluso
}
}
}
} ----------------------------------------------------
} Elena BELLUSO
} Dipartimento di Scienze Mineralogiche e Petrologiche
} Via Valperga Caluso, 35
} I-10125 TORINO - ITALIA
} tel:(39) 011 670 7135 - fax: (39) 011 670 7128
} e-mail: belluso-at-dsmp.unito.it
} http://www.dsmp.unito.it
} ----------------------------------------------------
}
}



From daemon Mon Jul 31 11:44:53 2000



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Mon, 31 Jul 2000 09:34:02 -0700
Subject: Re: Re[2]:SEM spot size & x-ray analysis

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Dear Gary,
I had a look at your image and I recently had a similar problem with the
analysis of fine layers in a GaAs/Al device. I was able to get good analyses
by lowering the acc. voltage to 3 to 5 kV. In that case I used the L lines
of Ga and As and the K line of Al to probe each layer in turn. My Monte
Carlo program tells me that the x-ray generation volume for carbon, aluminum
and silicon is 0.2 microns in depth and diameter at 3 kV, giving you the
resolution you need to analyse the edge and centre of the gate, assuming
that C, O, Al and Si are all the elements present. The 3 kV is almost a
doubling of the highest energy line analysed (1.74 keV Si Ka line) and
should excite all the lines you need.
At 08:29 PM 7/27/00 -0700, you wrote:

} At 03:50 PM 7/27/00, you wrote:
} } Since so many variables come into play, it is not surprising that details
} } are a bit vague in the book. Haven't referred to it in quite a while and
} } I forget the exact wording...
} } Taming the variables is where the statistical software is handy.
} }
} } I can't imagine differing lens construction making a difference in the
} } analysis volume other than a conical lens permitting higher tilt angles
} } for certain specimens which will cause the volume to skew along the
} } surface (the incident beam angle variable). Have I missed something?
} }
} } Since the beam diameter is typically MUCH smaller than the analysis
} } volume, variations in its size will not significantly affect the
} } x-ray analysis resolution. One exception to this is WDS analysis.
} } Sometimes HUGE beam currents are desired. This can lead to a(fat)beam
} } that will limit x-ray resolution. Generally speaking, if you can see a
} } crisp SE image at the desired magnification, the lens configuration
} } and beam diameter are not part of the analysis volume equation.
} } At this point you may or may not have enough beam current to achieve
} } the desired count rate.
} } Rule of thumb: Only if you must increase the beam current/spot size
} } (to raise the count rate) until the SE image is no longer well resolved
} } is it time consider beam size affecting the outcome.
} }
} } Woody
}
} The link below shows what I am trying to analyze.
}
} http://photoweb.net/ICxsect.jpg
}
} I know that PSG, Al and poly si are correctly marked. The poly
} gate is what is confusing me. Its contrast is like the surrounding
} oxide while its interior is like the poly layer....or it could be metal
} over poly.
}
} Based on these rather small feature sizes, will quantitative x-ray
} analysis produce viable results? If I probe the small central
} area of the gate, will I get the results of that area or the results
} of it and the surrounding material?
}
} gary g.
}
Regards,
Mary
Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca




From daemon Mon Jul 31 11:45:14 2000



From: Rick Harris :      raharris-at-ucdavis.edu
Date: Mon, 31 Jul 2000 09:33:29 -0700
Subject: Re: Monitor for imaging

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} I'm seeking recommendations for 20-21 color monitors to use for histological
} image analysis. Important parameters are high resolution and color
} reproduction, refresh rates of at least 85Hz and , of course, reliability.
} I've heard some good things about Viewsonics and the Sony GDM-500PS. Are
} there any I should avoid?
}

I use Viewsonic P185 monitors. I have 7 of them. I understand there is an
updated model (perhaps the P817?). I much prefer them to Sony. Sony uses
an aperture grill supported by wires that create two very fine lines
visible on the screen. We use the monitors at our graphics workstations,
on our Hitachi S3500n SEM and on our Gatan CCD camera on the Philips TEM.



Rick A. Harris, Director
Microscopy and Imaging Facility
Section of Molecular and Cellular Biology
1241 Life Sciences Addition
University of California
Davis, CA
530 752 2914
530 754 7536 fax
http://katie.ucdavis.edu
raharris-at-ucdavis.edu



From daemon Mon Jul 31 12:36:54 2000



From: beth-at-dogwood.botany.uga.edu (Beth Richardson)
Date: Mon, 31 Jul 2000 13:30:01 -0500
Subject: Nikon CoolPix 990

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Just wanted to add my 2 cents worth...I agree with Jonathan Dunlap - the
Nikon CoolPix 990 is a great digital camera. Ours was purchased for
departmental use so it's used out in the field, on the copy stand, etc.
Everyone has been very pleased with the images.
best regards,
Beth

**************************************
Beth Richardson
EM Lab Coordinator
Botany Department
University of Georgia
Athens, GA 30602

Phone - (706) 542-1790
FAX - (706) 542-1805
Email - beth-at-dogwood.botany.uga.edu
**************************************





From daemon Mon Jul 31 12:36:54 2000



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Mon, 31 Jul 2000 11:15:46 -0600
Subject: Re: Monitor for imaging

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I just saw that Bruce mentioned measurements with a ruler on the computer
screen:

I don't think, that is a viable method of measuring something. The glass on
the monitors is quite thick to withstand the enormous forces at the center
of the screen (at 1kg/cm2 the screen glass has to be thick!!). Since the
image is created on the inside, while the ruler is on the outside, the angle
at which you look at the ruler plays a significant role. Just place a ruler
on the screen and move your head. The measurements will change by much more
than the distortion of the screen (unless it's really bad).

For accurate measurements, one should always (in my opinion) use software to
do the measurements. That makes the measurement completely independent of
display devices.

Michael

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Bruce Brinson [mailto:brinson-at-cnst.rice.edu]
Sent: Saturday, July 29, 2000 9:35 PM
To: Connolly, Brett
Cc: 'MSAMicroscopy'


Hello Brett,
I personally have a PS 790 & it's predecessor (both 19" View Sonic). I
am
quite happy with them. Max. refresh rates are 90hz at 1280x1024 & 76Hz at
1600x1200.... a no $ interest disclaimer applies here.
The price of large monitors has dropped significantly which puts good
monitors in the price range of not so good units.
When looking at large monitors one thing to be weary of is a spatial non
linearity across the screen (distortion). Unless your trying to make
measurements on the screen with a hand ruler this is generally not
noticeable.
The larger the monitor, the more difficult this is to control. It is not
uncommon to find small magnets glued to the CRT. This is obviously not an
adjustable parameter. An easy way to look for/qualify the problem is to look
to
see if a round object in the middle is round or oval by the edge. This can
vary
with individual monitors. I have an expensive 19" (not VS) that has this
problem
to a greater extent than my 2 VS monitors in which it exist just a bit.
Actually
I hadn't even checked them 'til just now.
The other thing I have run it to (and not recently) is color or
brightness
non-uniformity across the screen. Again this is often not noticed in normal
computer applications. Try putting up a white screen & working with the
brightness & contrast to check for this problem.

good luck,
Bruce Brinson
Rice U.


"Connolly, Brett" wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} I'm seeking recommendations for 20-21 color monitors to use for
histological
} image analysis. Important parameters are high resolution and color
} reproduction, refresh rates of at least 85Hz and , of course, reliability.
} I've heard some good things about Viewsonics and the Sony GDM-500PS. Are
} there any I should avoid?
}
} Thanks,
} Brett
}
} Brett M. Connolly, Ph.D.
} Merck Research Laboratories
} Department of Pharmacology
} WP26A-3000
} PO Box 4
} West Point, PA 19486
} Ph. 215-652-2501
} FAX 215-652-2075
} e-mail: brett_connolly-at-merck.com
}




From daemon Mon Jul 31 17:39:36 2000



From: Wolfgang Ziegler :      ziegler-at-princeton.wireline.slb.com
Date: Mon, 31 Jul 2000 18:27:24 -0400
Subject: Re: Nikon Coolpix 990

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Jonathan,

we have several CoolPix here with adapters for different scopes - the folks at Diagnostic Instruments are very helpful in discussing and selecting components. You can then purchase through one of their distributors. Be aware that depending on your microscope you'll need some relatively expensive components to couple to the photo port because the CP has a non removeable lens. For others it is as simple as an adapter tube.
Make sure you also get the Nikon shutter release bracket (unless the 990 has a remote shutter release - the 800/950 doesn't)

regards,
Wolfgang Ziegler



At 10:34 AM 7/31/00 -0400, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From daemon Mon Jul 31 18:42:15 2000



From: Maria Luiza :      luiza-at-ppgem.faenquil.br
Date: Mon, 31 Jul 2000 18:31:03 -0500
Subject: Grain size.......

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Hi. I am a studant from Brazil and I´m trying to measure the grain size of
my samples (They are Niobium 9%Tantalun).
I know there are a lot of softwares available on the internet, but I don´t
know where and if there is a specific program to analise the grain size of
metals.
Please, remember that I work at Brazil, so I don´t have any money buy a
software. We allways try to do the best we can.

Thank you very much..... João Paulo Barros Machado.....

Please answer this email for: jpbm-at-easygold.com.br





From daemon Mon Jul 31 18:45:30 2000



From: Walck, Scott D. :      walck-at-ppg.com
Date: Mon, 31 Jul 2000 19:36:55 -0400
Subject: Emispec users meeting -Interest is needed and it will fly. Please

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A few weeks ago, I asked if there was interest in having an Emispec Users
Group meeting at M&M. Emispec is willing to have one if there is interest.
So far, I have not received very many replies. I will have to let Emispec
know what the interest level is and if there isn't much, it will not fly.

If you have an Emispec system and you have an interest in having this
meeting at M&M, please let me know.

Please pass this message on to Emispec users and have them respond to me.

If you already responded to me, it wouldn't hurt to do so again.

Thanks,


-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)





From daemon Mon Jul 31 19:55:31 2000



From: Steve Beck :      becks-at-sunynassau.edu
Date: Mon, 31 Jul 2000 20:48:23 -0400
Subject: Fall 2000 - TEM Course Announcement

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FALL 2000 COURSE ANNOUNCEMENT - Transmission Electron Microscopy (BIO. 221-E2)

NASSAU COMMUNITY COLLEGE (Long Island, NY)

A fourteen week, Fall 2000 semester, course in Biological Transmission
Electron Microscopy is being offered by the Biology Department of Nassau
Community College. This is a 4 credit course offered ONE EVENING PER WEEK,
Thursdays, starting at 5:30pm. Classes will begin on Sept. 7 and end on
Dec. 14, 2000.

This is a "hands-on" course emphasizing biological specimen preparation,
ultra-thin sectioning involving block trimming, glass knifemaking and
operation of the ultramicrotomes (Sorvall MT-2B and LKB Ultrotome III),
thick and ultra-thin section mounting and contrast staining (UA and Pb
citrate), grid support films (formvar, carbon), student operation of the
TEM (Hitachi HS-8, Philips EM 300) and production of electron micrographs
through the process of black & white photography, and electron micrograph
analysis. Students will work on a chosen sample(s) with the goal of
producing a portfolio of high quality TEM photomicrographs of that
sample(s).

The course is widely transferrable and the cost per credit is reasonable at
$86 per credit.

More information about the Bio-Imaging Center at NCC, course descriptions
and syllabi, and the beginnings of a student gallery of EM photomicrographs
is available at our web site. The URL is
{http://www.sunynassau.edu/webpages/biology/becks.htm} .

For those without www access, the catalog description is specified below.
If you have further questions, you should e-mail me directly at the address
below.

Interested individuals should register early (by Aug. 10) since the course
is limited to a total enrollment of ten (10) students.

Questions regarding the actual registration process can be directed to our
registrar at (516) 572-7355.
________________________________________________________________________________


CATALOG DESCRIPTION
BIO 221: Transmission Electron Microscopy -- 4 credits
Prerequisites: BIO 109-110 (Intro. Bio.) or equivalent, CHE 151-152
(Inorganic Chem.)
An introduction to the basic principles of transmission electron
microscopy including tissue preparation, microscope (TEM) operation, black
& white photography, and micrograph interpretation. The entire laboratory
is devoted to the development of skills and preparative techniques involved
with the operation of an actual transmission electron microscope.
(3 lecture, 3 laboratory hours). Laboratory fee applies.
________________________________________________________________________________







Stephen J. Beck
Associate Professor
Bio-Imaging Center/Electron Microscopy
Department of Biology
Nassau Community College
Garden City, NY 11530
Voice Mail: (516) 572-7829
Email: {becks-at-sunynassau.edu}
URL: {http://www.sunynassau.edu/webpages/biology/becks.htm}





From daemon Mon Jul 31 21:24:43 2000



From: Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 31 Jul 2000 19:07:20 -0700
Subject: Re: Re[2]:SEM spot size & x-ray analysis

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Thanks very much to all who have responded to this initial
posting. It seems to me that the data I have received confuses
me for than before. But the ideas presented are great to
consider. Proving them out is quite another matter.

At 09:14 AM 7/30/00, you wrote:
} Gary,
}
} It is common for IC manufacturers to use metals in direct contact with poly
} gates thus reducing the sheet resistance of the poly especially for
} interconnects such as in your image. It could be that the similar contrast
} means a titanium-silicide or something like that. Therefore, to excite the
} higher x-ray lines will require higher beam voltage and you are correct in
} wondering about the volume which is generating the detected x-rays.
}
} I have had much better success analyzing semiconductor cross sections with
} Auger than EDS. Auger's built-in ion gun sputtering provides a good means
} of cleaning the face of the cross section, the resolution (for imaging and
} analyzing) is certainly adequate for this size structure and once you have
} identified the elements present over an area, a line scan nails the elements
} present in the gate.
}
} I have some question about the labels on the photo. Are you really sure
} about those?


I offer a second image at

http://photoweb.net/icxsect2.jpg

which is a different and augmenting image of the IC structure.

I am not sure of the identification of each layer but I am
pretty sure of them. However, I can be dissuaded by alternate
opinions. The main point here is I think to keep in mind
that this is a fifteen year plus construction...it is not modern.
Therefore, what would be vogue or state-of-the-art back then?

The ability of x-ray analysis to quantify the various layers
seems to be easy or impossible, based on the feedback I
have received. On first blush, it does seem difficult based
on the small geometric area available for probing. I do not
yet have an x-ray capability but will be receiving one soon.
It is a 133eV light element dewar system. It will be interesting
to see how it performs in this application.

gary





From daemon Mon Jul 31 21:37:33 2000



From: Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 31 Jul 2000 19:21:36 -0700
Subject: IC structure

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I forgot to add the URL to a second image. Here it is:

http://www.photoweb.net/icxsect2.jpg

This and the previous image are BSE. The Z contrast
in these images is telling, I think. The problem is that
they are not conclusive. The particular problem is
the area at the substrate surface. I think that this is
a gate area, surrounded by field oxide. But I am
not sure. What is really troubling is the central
portion of the structure in the center of the image.
It has the same contrast as the poly Si....but how
could such a structure be made?

A puzzle.

gary g.



From daemon Tue Aug 1 06:34:49 2000



From: dqnez-at-Chat.ru
Date: Tue, 01 Aug 2000 07:21:56 -0400
Subject: Serious About Retiring in 2 to 5 Years? 13130

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Tired of the 40 X 40 X 40 Plan? You know:










From daemon Tue Aug 1 07:42:39 2000



From: Paciej,Richard :      Richard.Paciej-at-us.gases.boc.com
Date: Tue, 1 Aug 2000 07:32:07 -0500
Subject: Job For Electron Microscopist/Materials Engineer

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BOC Gases, has a unique opportunity for an Electron Microscopist/Materials
Engineer located at it's state of the
art Technical Center in Murray Hill, NJ.

BOC Gases, a division of The BOC Group Inc., was founded in 1888 and is a
global leader in the manufacturing
and distribution of industrial, medical, and specialty gases and related
equipment. Poised for new growth
opportunities, BOC currently operates in more than 50 countries, providing
customers with gases and technology
which are used to support life, freeze food, forge steel, shape glass,
refine petroleum, create computer chips, and
clean wastewater and smokestack emissions, in addition to hundreds of
other applications.

Electron Microscopist/Materials Engineer

Responsibilities

The successful candidate will support the materials characterization needs
of the Gases Technology R&D
organization using and maintaining analytical equipment, such as, Scanning
Electron Microscopy, X-ray Diffraction.
Metallography. Characterization includes metallic surfaces,
adsorption/catalysis materials as well as other materials
related to gases delivery equipment and vessels. This position is within
the analytical services part of the Materials
Technology Group. More specifically, the person will have the
responsibility to:

Provide scanning electron microscope (SEM), environmental scanning
electron microscope (ESEM), and
metallography services.

Maintain the SEM and ESEM equipment in good operating condition.

Develop new techniques to support researchers needs.

Perform failure analysis and provide technical assistance for material
selection projects.

Experience/Education

A B.S. or M.S. in the field of materials science and engineering with
practical working experience with
scanning electronic microscope techniques. Experience in x-ray diffraction
a plus.

A knowledge of a wide variety of materials. Experience with mechanical
testing, failure mechanisms,
corrosion, and materials, especially metals and polymers.
5-10 years work experience in a materials laboratory.

Work experience in the chemical processing industry a plus.

Ability to perform failure analysis.

Ability to multitask is essential.

Send application to: BOC Gases, Attn: Recruiting - SEM-NM, 575 Mountain
Avenue, Murray Hill, NJ
07974. E-mail: jobs-at-us.gases.boc.com


fax: 908-771-1702. Along with opportunities for professional growth
we offer competitive compensation and comprehensive benefits.




BOC Gases is an equal opportunity employer.


*********************************************************************
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the presence of known computer viruses by the MessageLabs Virus
Control Centre. However, it is still recommended that you use
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From daemon Tue Aug 1 09:25:43 2000



From: Matt Ervin :      mervin-at-arl.army.mil
Date: Tue, 1 Aug 2000 10:13:23 -0400
Subject: EDS resolution

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



When I had resolution problems recently (which turned out to be due to a
"leaky" detector) one of the first things my service engineer asked me to
check was to make sure that the detector wasn't touching the pole piece
(which can cause a ground loop?) Try backing the detector out a little to
see if the peak width changes. I doubt it will help, but it is easy to
try.
Matt




"E. J. McKenzie" {elizm-at-pdx.edu} on 07/28/2000 12:30:59 PM





To: microscopy-at-sparc5.microscopy.com
cc:


Hi everyone,

we are having serious problems with the resolution of our EDS detector -
the
peak height-width ratio is terrible, around 450 counts. Consequently, our
peaks look more like rolling hills and one peak blurs into another. I have
callibrated the machine using an Al-Cu standard, checked that the detector
is kept cool, sample is tilted at 30 degrees, etc etc. I also got another
microscopist to come and have a look to check my methods and he also
concluded there was a serious problem.

Is there anything else I could try before I phone the Noran technicians and
pay them a lot of $$$ to fix/replace the detector?

regards
Liz McKenzie
--------------------------------------------------
Geomicrobiology and Electron Microscopy Laboratory
Room S9 Cramer Hall
1721 SW Broadway
Portland State University
Portland
OR97201

ph:503 725 3362
fax:503 725 3025








From daemon Tue Aug 1 09:32:54 2000



From: Andy Horsewell :      horsewell-at-ipt.dtu.dk
Date: Tue, 01 Aug 2000 16:21:57 +0200
Subject: subscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html







From daemon Tue Aug 1 10:57:55 2000



From: Simon C. Watkins :      swatkins+-at-pitt.edu
Date: Tue, 01 Aug 2000 11:41:02 -0400
Subject: microm service manual

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Folks does anyone know where I could obtain a service manual (not a user
manual) for a Microm 505 cryostat
Tx
Simon


----------------------------------------------
Simon C. Watkins Ph.D. MRC Path
Associate Professor Cell Biology and Physiology
Director Center for Biologic Imaging
University of Pittsburgh
BSTS 225
3500 Terrace ST
Pittsburgh PA 15261
Tel:412-648-3051
Fax:412-648-8330
URL: http://sbic6.sbic.pitt.edu
--------------------------------------------




From daemon Tue Aug 1 11:39:58 2000



From: Windland, Mark J (MN14) :      Mark.Windland-at-honeywell.com
Date: Tue, 1 Aug 2000 11:38:56 -0500
Subject: RE: Re[2]:SEM spot size & x-ray analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We manufacture semiconductors. I work in the Analytical Services
cross-sectioning these daily. These images you sent are of a bulk silicon
process. To best describe the layers, I will start at the silicon and work
my way up. On the second picture the field oxide comes to a point in two
locations allowing the metal above to make contact to the silicon. The first
picture shows this layer as the full thickness along the silicon. The next
layer is the 1st metal(not poly silicon) that is making a contact in the
second image and just a first metal line on the first image. The metal has
a undoped oxide over it that gives the different z contrast. Then there is
an oxide over that, could be PSG. Then there is a second metal
layer(labeled Poly Si on the first picture). On top of that is a layer of
oxide(which is labeled Al on the first picture). Then on top of everything
is either a nitride or polyimide capping the chip.
The metals are easy to identify because of the grain structure.
There are not any gates shown on these two photos.
Gary if you would like to give me a call I can explain the layers in
more detail. I can also give you information on how they are processed and
what to look for.

Mark Windland
Honeywell
Solid State Electronics Center
Plymouth, Minnesota
612-954-2845
-------------------
} Thanks very much to all who have responded to this initial
} posting. It seems to me that the data I have received confuses
} me for than before. But the ideas presented are great to
} consider. Proving them out is quite another matter.
}
} At 09:14 AM 7/30/00, you wrote:
} } Gary,
} }
} } It is common for IC manufacturers to use metals in direct contact with
} poly
} } gates thus reducing the sheet resistance of the poly especially for
} } interconnects such as in your image. It could be that the similar
} contrast
} } means a titanium-silicide or something like that. Therefore, to excite
} the
} } higher x-ray lines will require higher beam voltage and you are correct
} in
} } wondering about the volume which is generating the detected x-rays.
} }
} } I have had much better success analyzing semiconductor cross sections
} with
} } Auger than EDS. Auger's built-in ion gun sputtering provides a good
} means
} } of cleaning the face of the cross section, the resolution (for imaging
} and
} } analyzing) is certainly adequate for this size structure and once you
} have
} } identified the elements present over an area, a line scan nails the
} elements
} } present in the gate.
} }
} } I have some question about the labels on the photo. Are you really sure
} } about those?
}
}
} I offer a second image at
}
} http://photoweb.net/icxsect2.jpg
}
} which is a different and augmenting image of the IC structure.
}
} I am not sure of the identification of each layer but I am
} pretty sure of them. However, I can be dissuaded by alternate
} opinions. The main point here is I think to keep in mind
} that this is a fifteen year plus construction...it is not modern.
} Therefore, what would be vogue or state-of-the-art back then?
}
} The ability of x-ray analysis to quantify the various layers
} seems to be easy or impossible, based on the feedback I
} have received. On first blush, it does seem difficult based
} on the small geometric area available for probing. I do not
} yet have an x-ray capability but will be receiving one soon.
} It is a 133eV light element dewar system. It will be interesting
} to see how it performs in this application.
}
} gary
}
}



From daemon Tue Aug 1 11:42:21 2000



From: E. J. McKenzie :      elizm-at-pdx.edu
Date: Tue, 01 Aug 2000 09:29:20 -0700
Subject: Kevex EDX

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello everyone

Does anyone out there have a Kevex-ray EDX detector, 2003 model? We don't
have a manual (or any literature, for that matter) and I just wondered if I
could obtain a photocopy or acrobat file from someone. I've also contacted
Noran.

Thanks for all the hints on fixing the peak problems - it seems to be
working fine now (210eV FWHM for Mn peak), I just have to figure out what
went right!

regards
Elizabeth McKenzie
--------------------------------------------------
Geomicrobiology and Electron Microscopy Laboratory
Room S9 Cramer Hall
1721 SW Broadway
Portland State University
Portland
OR97201

ph:503 725 3362
fax:503 725 3025




From daemon Tue Aug 1 12:24:07 2000



From: milesd-at-us.ibm.com
Date: Tue, 1 Aug 2000 13:12:39 -0400
Subject: Re: IC structure

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Gary,

I did not comment on your last image, as I needed more info. before making
a statement. The second image is easy to comment on. The gate structure
is the line which is the same contrast as the substrate. It starts towards
the
left of the image, over "field oxide", drops down over the "birds beak" and
continues across the gate oxide. Then it rises over the other "birds
beak",
and terminates over the "field oxide". The darker thin line at the surface
of
the substrate (connecting the two "bird beaks") is the gate oxide. The
gate
appears to be polysilicon, but I would need more information to say for
sure.

Darrell




From daemon Tue Aug 1 12:49:04 2000



From: john david whitaker :      jwhitake-at-u.washington.edu
Date: Tue, 1 Aug 2000 10:39:52 -0700 (PDT)
Subject: Insoluble residue after polishing

Contents Retrieved from Microscopy Listserver Archives
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Dear Listservers,

I am having problems removing a white, spotty, crystalline-
like residue from my XTEM sample. Has anyone out there
experienced similar problems? Any suggestions would be
appreciated. I summarize my prep procedure below, as
well as the methods I used to try to remove the residue. (It
should be noted that my sample is a sandwich sample
prepared using Gatan G-1 epoxy.)

1. Mount sample on pyrex polishing stub with superglue.
2. Polish with alumina lapping films.
3. Final polish with polycrystalline diamond suspension
on a final polishing cloth.
4. Soak in acetone to dissolve superglue and release
sample from stub.

After the above procedure there was the aforementioned
residue. I attempted removing it by the following methods:
1. Rinsed/soaked in ethanol.
2. Rinsed/soaked in water.
3. Rinsed/soaked in acetone overnight.
4. Rinsed/soaked in hexane.
5. Sonicate in acetone.
6. Sonicate in water.

Again, none of these methods seemed to work.

Any suggestions?

Thanks,

John




From daemon Tue Aug 1 15:43:31 2000



From: Winnie Westbrook :      ewwestbr-at-hsc.vcu.edu
Date: Tue, 01 Aug 2000 16:32:44 -0400
Subject: subscribe

Contents Retrieved from Microscopy Listserver Archives
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From daemon Tue Aug 1 15:43:32 2000



From: anderron-at-us.ibm.com
Date: Tue, 1 Aug 2000 16:34:12 -0400
Subject: Phys Sample Prep Super Glues

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} From time to time we have recommended cyanoacrylate super glues for use
with tripod polishing and related methods. The trouble with using consumer
products is that they sometime change without warning. We have experienced
poor results with glues we have recommended in the past. No point in
naming names. After performing an evaluation on replacement glues, we now
recommend:


1). RP30 CYANOACRYLATE ADHESIVE - $6.75/1 oz. bottle

by AMERICAN CHEMICAL CO.
2630 South Irving Avenue
Minneapolis, MN 55408-1047
(612)-374-1767
Contact: Bill Reker
FAX: (612)-377-3590
Web: www.glueit.com
E-mail: info-at-glueit.com


2). 310035 INSTANT SUPERGLUE ETHYL CYANOACRYLATE -
$5.98/1 oz. bottle

by ND INDUSTRIES
750 Wylly Avenue, Suite 3
Sanford, Fl. 32773
(800)-521-2663
Contact: Laurie Russell

Sold to us via: Moreau Marketing & Sales
501 Shepherd Street, Suite #4
Winston Salem, NC 27103
Contact: Robert Moreau
(336)-659-6674
FAX: (336)-659-6683

For further details, please contact Mark Hudson at (845)-892-2441
(currently
using RP30) or Stan Klepeis at (845)-892-2192 (currently using 310035).



Ron Anderson, IBM, Hopewell Jct., New York, USA. anderron-at-us.ibm.com

IBM Analytical Services; http://www.chips.ibm.com/services/asg




From daemon Tue Aug 1 16:20:34 2000



From: Doug Cromey :      doug-cromey-at-ns.arizona.edu
Date: Tue, 01 Aug 2000 14:24:52 -0700
Subject: silver staining problems with comet assay

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Mr. Whitaker,

It seems as if you may be contaminating your sample with the superglue after
using the acetone to clean the sample. However, what you describe sounds
much like colloidal silica particles.

Try using IPA or methanol after acetone. Acetone leaves residue on samples
and does not come clean easily.

If for some reason you neglected to mention the use of colloidal silica, I
am 98 percent sure that is what you see. If that is the case, MicroOrganic
Soap works well to remove colloidal silica from samples. You may also wish
to switch the type of colloidal silica you are using.

I hope this works for you.

Sincerely,

Gary Liechty
Manager, Technical Products

Allied High Tech Products, Inc.
2376 E. Pacifica Place
Rancho Dominguez, CA 90220

310-635-2466
310-762-6808 Fax
800-675-1118 Toll Free, US and Canada

www.alliedhightech.com

Equipment and Consumables for Metallurgical Sample Preparation
----- Original Message -----
} From: "john david whitaker" {jwhitake-at-u.washington.edu}
To: "MSA listserver" {Microscopy-at-sparc5.microscopy.com}
Sent: Tuesday, August 01, 2000 10:39 AM


Dear Microscopy Listserv,

I have a colleague that is doing a light microscopic comet assay
(single cell gel electrophoresis) on glass slides using
respiratory cells from sputum. She has been using silver
staining (w/ a kit from Trevigen) and is having difficulty
getting clean preps (ie without precipitates or extraneous
staining). Does anyone have any tips or suggestions? She's
tried doing the assay with fluorescent preps on our confocal
microscope, but the specimen fades too quickly to acquire useful
data.

Yours,
Doug
....................................................................
: Douglas W. Cromey, M.S. Dept. of Cell Biology & Anatomy :
: Research Specialist, Principal University of Arizona :
: (office: AHSC 4212A) P.O. Box 245044 :
: (voice: 520-626-2824) Tucson, AZ 85724-5044 USA :
: (FAX: 520-626-2097) (email: doug-cromey-at-ns.arizona.edu):
:...................................................................:
http://swehsc.pharmacy.arizona.edu/exppath/
Home of: "Microscopy and Imaging Resources on the WWW"




From daemon Tue Aug 1 16:48:16 2000



From: John C. Wheatley :      John.Wheatley-at-asu.edu
Date: Tue, 01 Aug 2000 14:45:30 -0700
Subject: Job Available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Arizona State University
Center for High Resolution Electron Microscopy
Research Specialist

The Center for High Resolution Electron Microscopy at Arizona State
University has an open position for a Research Specialist. The Center
provides electron microscopy resources for the university and external
community, including a range of transmission and scanning electron
microscopes, as well as specimen preparation facilities. The successful
applicant will be expected to assist with the day-to-day operation and
maintenance of microscopes within the Center. Primary instrument
responsibility will include but not be limited to the JEOL 2000FX
transmission electron microscope (TEM), the JEOL 840 Scanning EM, and the
soon-to-be acquired JEOL 6300 SEM with liquid-helium CL system. Other tasks
will include training and supervision of researchers in proper use of the
instruments, including observance of safety procedures, and authorization
of users. Some limited opportunity to assist and advise researchers in
designing and carrying out experiments, including active participation and
collaboration in experiments with data collection and analysis as required.

Minimum qualifications: Bachelors or Masters degree or equivalent training
in Physical or Materials' Sciences or closely related discipline, with at
least three but preferably five years' additional experience with operation
and maintenance of scanning and/or transmission electron microscopes.
Experience with design, construction and testing of electronic circuitry,
and knowledge of vacuum systems would be desirable.

Application deadline: August 15, 2000, or until position filled.

Arizona State University is an equal opportunity employer. Women and
minorities are encouraged to apply.

Send resume and the names and addresses of three references to John Wheatley.
Please contact John via one of the following ways--preferably e-mail:

John C. Wheatley, Lab Manager
Arizona State University
Center for Solid State Science
Tempe, AZ 85287-1704

Phone: (480) 965-3831
FAX: (480) 965-9004
John.Wheatley-at-ASU.Edu


John C. Wheatley
Lab Manager
Arizona State University
Center for Solid State Science
PSA-213
BOX 871704
Tempe, AZ 85287-1704


Phone: (480) 965-3831
FAX: (480) 965-9004
John.Wheatley-at-ASU.Edu





From daemon Tue Aug 1 18:18:54 2000



From: Gen Pei :      gp35-at-cornell.edu
Date: Tue, 1 Aug 2000 19:09:28 -0400
Subject: how to unsubscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thanks. Gen
******************************************************************
Gen Pei
Department of Materials Science and Engineering
Cornell University
328 Thurston Hall
tele: (607)255-5177
fax:(607)255-2365
gp35-at-cornell.edu

******************************************************************



From daemon Tue Aug 1 23:30:49 2000



From: Nelson Conti :      NelsonC51-at-excite.com
Date: Tue, 1 Aug 2000 21:18:45 -0700 (PDT)
Subject: Re: how to unsubscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



On Tue, 1 Aug 2000 19:09:28 -0400, Gen Pei wrote:

| ------------------------------------------------------------------------
| The Microscopy ListServer -- Sponsor: The Microscopy Society of America
| To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
| On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
| -----------------------------------------------------------------------.
|
|
| Thanks. Gen
| ******************************************************************
| Gen Pei
| Department of Materials Science and Engineering
| Cornell University
| 328 Thurston Hall
| tele: (607)255-5177
| fax:(607)255-2365
| gp35-at-cornell.edu
|
| ******************************************************************
|


Nelson Conti
164 Ferne Court
Palo Alto, CA 94306

Gen Pei-
I've unsubscribed myself one time. I believe that you need to send an
e-mail to the MSA Listserver (address = ? ), and just indicate "unsubscribe"
in the subject heading. To find the address, go to the MSA Listserver Web
address, and it should be possible to find the e-mail address for the MSA
Listserver. Sorry, I don't recall it off-hand.
Good luck with unsubscribing.
Nelson Conti
P.S. When in doubt - you can also re-send your message, but direct it to
Nestor Zaluzec. I'm sure that he can tell you precisely how to unsubscribe.





_______________________________________________________
Say Bye to Slow Internet!
http://www.home.com/xinbox/signup.html




From daemon Wed Aug 2 07:39:35 2000



From: Mick Thomas :      mgt3-at-ccmr.cornell.edu
Date: Wed, 02 Aug 2000 08:24:49 -0700
Subject: wireless lan interference

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Fellow microscopists,

Have any of you had any experience with the installation of a wireless lan
affecting the operation of your microscopes in any way? I would very much
appreciate your advice in this regard.

Sincerely,

Mick Thomas
-------------------------------------------------
Mick Thomas
UHV-STEM Laboratory
E-1 Clark Hall
Cornell University
Ithaca, NY 14853

Phone: 607-255-0650
Fax: 607-255-7658
e-mail: mgt3-at-ccmr.cornell.edu



From daemon Wed Aug 2 07:48:56 2000



From: Alwyn Eades :      jae5-at-lehigh.edu
Date: Wed, 02 Aug 2000 08:40:14 -0400
Subject: Astromed CCD camera

Contents Retrieved from Microscopy Listserver Archives
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From daemon Wed Aug 2 08:38:50 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Wed, 2 Aug 2000 08:30:36 -0500
Subject: Re: wireless lan interference

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have not yet seen any effects, with the Apple Airport system installed
for my computers as well as a GigaHertz wireless broadcasting systems which
are
carrying video signals from one room to another.

The Computer monitors however are a different story.

Nestor



} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America






From daemon Wed Aug 2 09:57:09 2000



From: Alwyn Eades :      jae5-at-lehigh.edu
Date: Wed, 02 Aug 2000 10:46:13 -0400
Subject: Astromed CCD camera

Contents Retrieved from Microscopy Listserver Archives
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Sorry for the previous blank message. Nestor thinks it was just a fluke.

We have an Astromed cooled CCD camera, which we would like to use.
However, we have no manuals for it. If you have manuals or other
information that would allow us to put this camera into operation please
let me know "off line".

Thank you,
Alwyn Eades
.

The camera has two labels:
Model number TE2W (serial 117)
Model number P86121 (serial 7451/8/14)

There are two boxes of electronics labelled:
Box 1
Model TE2/PSU (serial 102)
Box 2
Model DDE/2200 (serial 116)
Alwyn Eades
Department of Materials Science and Engineering
Lehigh University
5 East Packer Avenue
Bethlehem
Pennsylvannia 18015-3195
Phone 610 758 4231
Fax 610 758 4244
jae5-at-lehigh.edu




From daemon Wed Aug 2 10:27:57 2000



From: Shea Miller :      millers-at-em.agr.ca
Date: Wed, 02 Aug 2000 11:20:54 -0400
Subject: GFP

Contents Retrieved from Microscopy Listserver Archives
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Hello everyone;
I have just started looking at a GFP-transformed fungus growing on plant surfaces, using regular fluorescence microscopy. I can clearly see the fungus using two filter sets: a UV set (ex/em 365/} 420) and a blue filter set (ex/em 470/} 515). While the fluorescence in blue light is much brighter, stuff that is blue/blue-white with UV illumination is green in the blue light. is the "extra" green in the blue light likely to be where the GFP is not as concentrated, and hence doesn't show up in the UV, or is it more likely to be some other compound that has similar excitation/emission characteristics? I have done a fair bit of reading on the subject, but am still feeling a bit vague, and would really appreciate hearing from those of you who have some first hand experience with GFP.

thanks in advance
shea



Dr. S.Shea Miller
Agriculture & AgriFood Canada
Eastern Cereal and Oilseed Research Centre
Rm. 2068 Neatby Building
Central Experimental Farm
Ottawa, Ontario
Canada K1A 0C6
Phone: (613) 759-1760
Fax: (613) 759-1701
E-mail: millers-at-em.agr.ca




From daemon Wed Aug 2 11:39:23 2000



From: Rafael Pena/MONT3/MFG/KEMET/US :      RafaelPena-at-kemet.com
Date: Wed, 2 Aug 2000 11:20:28 -0500
Subject: SEM/EDS COURSES.

Contents Retrieved from Microscopy Listserver Archives
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Hi , i wanted to know if anyone knew of any SEM/EDS courses in Mexico or
Texas.
Thanks.

Rafael Peña.




From daemon Wed Aug 2 13:24:08 2000



From: anderron-at-us.ibm.com
Date: Wed, 2 Aug 2000 14:12:05 -0400
Subject: Phys Sample Prep Super Glues

Contents Retrieved from Microscopy Listserver Archives
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With regard to the posting I made on this subject, Chuck Garber sent me the
following note wherein he raises a number of important points. Chuck is
correct in all regards. The product we were using was not DURO. We have
no experience with Duro. We have made specific, brand name,
recommendations in the past and my note was to share our recent experiences
regarding these products. The products we were using were not "garage
level" products.

So, IF, and only IF, you are using a product solely because of a
recommendation from our lab, we now make new recommendations.

Ron

-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Hi Ron,

I guess I should give some kind of disclaimer statement first, that in no
way am I suggesting that what you are saying it not correct. If this was
your perceived experience, then I am sure it is correct and I am not
questioning it.

And it is a fact that there are some garage level operations where people
take from 5 gallon pales and "fill" the 1 oz plastic bottles and put on a
label and put it into distribution, without any real testing of the
integrity of their packaging or any of the other things you and I might
think one should be doing to insure the integrity of their product.

But I can assure you, without hesitation, the cyanoacrylate that is made by
Duro, which is indeed a consumer products company, but it is also "top in
class" when it comes to this type of product, has been offered by us (e.g.
SPI) for years and we have never ever had anyone every voice even the
slight
complaint about it. We also decided a long time ago that once opened, the
shelf life of these products can be very short, and consequently, we
decided
to offer the product in the smaller 3g package.

I go through the trouble to point this out to you because I see things from
a slightly different perspective possibly than you do:

1] For one thing, someone even at IBM, having to place an order with
either
of the two mentioned companies, given the cost of placing an order period
of
any size, what is the overhead going to be? But if someone was ordering
the
Duro product from someone like SPI, then it just gets added in with a bunch
of other things and the real cost is almost nothing.

2] For someone in Mexico, the situation is far worse. You have a lot of
influence on people because of your reputation. Your posting could make
someone in Mexico City think that in order to get acceptable results, they
have to place a direct order in the USA for one of these "special"
cyanoacrylates and in situations with limited research budgets, it just
seems to me like they have better ways to be spending their money.


So while the Duro product might not be any better than the two mentioned,
the point is that because of the distribution and transportation costs, if
people are led to believe that they really do need something that can not
be
procured from their traditional sources of purchasing, they end up wasting
a
lot of money unnecessarily.

Of course if you had any hard evidence that the Duro product was in some
way
inferior, that would surely be another story. But chemically, these are
all
the same identical materials, they all have the same identical MSDS
information and the all perform identically. When ever I have heard of
someone having problems it has been because they were using product from a
previously opened package.

Feel free to use this information however you feel might be approriate,
either in whole or in part. But I did want to share my views with you on
your posting, which I hope you won't mind my having done.......

Chuck



Ron Anderson, IBM, Hopewell Jct., New York, USA. anderron-at-us.ibm.com

IBM Analytical Services; http://www.chips.ibm.com/services/asg




From daemon Wed Aug 2 13:56:05 2000



From: Debby Sherman :      sherman-at-btny.purdue.edu
Date: 02 Aug 2000 13:47:24 -0500
Subject: CM-10 computer log-in

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Hi all,
We would like to add an individualized computer password system to our FEI/Philips CM-10 TEM. We would like a user to be able to type in their password which would then trigger a switch tied into the high tension and turn the HT on. The system would ideally then have a timer to record "on" time and a function to record this time with the user ID.
Connecting a timer to the high tension is not a problem...the computer password part is where we need help.
Please contact me if you have a system such as this on a FEI/Philips CM-10 or even on another make TEM which might work on a CM-10.
Thanks,
Debby

Debby Sherman, Manager Phone: 765-494-6666
Microscopy Center in Agriculture FAX: 765-494-5896
Dept. of Botany & Plant Pathology E-mail: sherman-at-btny.purdue.edu
Purdue University
1057 Whistler Building
West Lafayette, IN 47907-1057




From daemon Wed Aug 2 15:56:11 2000



From: milesd-at-us.ibm.com
Date: Wed, 2 Aug 2000 17:55:26 -0400
Subject: Re: IC structure

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Date sent: Wed, 02 Aug 2000 11:20:54 -0400
} From: "Shea Miller" {millers-at-em.agr.ca}
To: confocal-at-listserv.acsu.buffalo.edu, microscopy-at-sparc5.microscopy.com


Well, I guess Mark and I would have to see a higher
magnification image, detailing the polysilicon, the
"birds beak" area, and the substrate. This way we
could tell whether this is a gate, with gate oxide, or
a diffusion contact. I am assuming that your cross
section is well into the middle area of the polysilicon
line, and not just at the edge.

Darrell




From daemon Wed Aug 2 22:12:46 2000



From: Gary Gaugler :      gary-at-gaugler.com
Date: Wed, 02 Aug 2000 19:49:48 -0700
Subject: IC structure

Contents Retrieved from Microscopy Listserver Archives
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OK...I think that I have this figured out. I got some good
input from several folks who were right about some portions
and some who were perhaps wrong. So was I. But I think
that I have it sorted out now.

I really can't say that this is a double poly structure quite
yet until my x-ray system is available (Rontec 133eV SHV).
Even then, it may not be able to discern the constituents
in the small gate area. We'll see.

In the meantime, I will put an annotated pix on my site
which shows what I think is the real identification of each
layer. It still may not be 100% right....but I think that it
will be quite close.

gary g.




From daemon Thu Aug 3 04:43:01 2000



From: Philippe-Andre Buffat :      philippe.buffat-at-epfl.ch
Date: Thu, 3 Aug 2000 11:23:00 +0200
Subject: size effect on SAED patterns for platelets

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Hello,

I am looking for a programme (or a collaboration) to compute powder
XRD or SAED taking in account the size effect due to the platelet
shape of small crystals in a polycristalline (non textured?) powder
(hexagonal Bravais lattice).

In a first, simple, approach we can consider only the beam broadening
of the bulk reflections (‰+/size in that particular direction). A
more accurate computation would take in account that the smallest
crystals are no longer a sum of complete unit cells but a supercell.

Simulation of powder patterns with CrystalMaker or Carine show that
it exist a strong overlapping between reflections for isotropic
crystal some nanometers in size.

Do somebody know if such a programme is available or where somebody
could perform a few simulations for me?

Best regards

Philippe Buffat

__________________________________________________________________
Philippe Buffat
Ecole Polytechnique Federale de Lausanne (EPFL)
Centre Interdepartemental de Microscopie Electronique
Address: EPFL-CIME, Batiment MX-C, CH-1015 Lausanne, Switzerland
Phone: +41(21)693 29 83 Fax: +41(21)693 44 01 (Central European Time)
E-mail: philippe.buffat-at-epfl.ch, WWW URL http://cimewww.epfl.ch/
______________________________ Eudora F2.1 ___________________________



From daemon Thu Aug 3 06:41:19 2000



From: SGKCCK-at-aol.com
Date: Thu, 3 Aug 2000 07:28:45 EDT
Subject: M&M 2000-Special Events

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As we approach the upcoming meeting I would like to take this opportunity as
the LAC Chair to let you know that this meeting promises to be one of the
best yet. With a sell out of Exhibit space and pre registration growing
Philadelphia is the place to be this August. To all of our surprise, we have
sold out on most social events. We do have left just 75 Spirit of 76
Wednesday Evening Dinner Cruise tickets left which we would like to open up
to those of you whom have not purchased any. Joining this trip will afford
you the relaxing experience of floating down the Delaware River on an elegant
ship filled with wonderful food, and lively music. Sail by Penn's Landing,
Old Christ's Church, the Ben Franklin Bridge, the Flagship Olympia, and much
much more. The cruise includes a five entry buffet with all of the
condiments, salad, desserts and coffee and tea. There are 2 dance bands as
well as entertainment on board.
Transportation will be from all of the local hotels and convention center.
All of this on a usually boring Wednesday night for only $55 for everything.
Please E-mail me directly or call if you are interested so we may make all of
the arrangements.
I look forward to hearing from you.

Sincerely,
Stacie Kirsch, LAC Chair
215-646-1566



From daemon Thu Aug 3 08:22:30 2000



From: MGanger-at-aol.com
Date: Thu, 3 Aug 2000 09:01:54 EDT
Subject: AFM Work

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Greetings all,

Our university is looking to do some imaging of DNA and DNA/protein
interactions. We do not have a AFM and are looking to contact anyone in the
NJ, NY, CT, or PA area so that we could visit a lab and possibly contract out
some work or work in collaboration. If you are aware of any
company/university that has AFM, could you please contact myself or Jack
Gaynor at these email addresses: mganger-at-aol.com or
jack.gaynor-at-montclair.edu.

Thanks in advance,

Mike Ganger
Montclair State University
mganger-at-aol.com



From daemon Thu Aug 3 08:22:30 2000



From: Lance A Rodenkirch :      Larodenk-at-facstaff.wisc.edu
Date: Thu, 3 Aug 2000 08:08:04 -0500
Subject: Nikon parts

Contents Retrieved from Microscopy Listserver Archives
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Dear Sir/Madam:
Do you know of any vendors who sell older or discontinued Nikon parts?
I'm looking for a dic nosepiece for a diaphot 200? Any suggestions.
TIA

Lance Rodenkirch
Keck Imaging
UW-Madison





From daemon Thu Aug 3 09:55:19 2000



From: Jane LaGoy :      jlagoy-at-bodycote-imt.com
Date: Thu, 3 Aug 2000 10:47:11 -0400
Subject: Re: Polaroid Type 55 P/N film

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This message is in reply to Philip Oshel at U. of Wisconsin:

I've always been told that the brown goop that is easy to get on your hands
while pulling apart the photo & negative of the Polaroid T55 film is a known
carcinogen and to wash your hands immediately upon contact.

Jane L. LaGoy
Development Engineer
Bodycote IMT, Inc.
155 River Street
Andover, MA 01810
978-470-1620
jlagoy-at-bodycote-imt.com




From daemon Thu Aug 3 11:21:23 2000



From: Grizzi Fabio ICH :      fabio.grizzi-at-humanitas.it
Date: Thu, 3 Aug 2000 18:02:43 +0200
Subject: Fast Green

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Dear all

I would like to ask you how to stain non-collagenous fibres in
paraffin-embedded human tissues (i. e. liver), by using Fast Green stain.
Have someone the method?

I read some paper about this stain, but these reported a pre-incubation with
0.01% Fast green in saturated picric acid solution for 15 min, and then
after some washes a new incubation with Fast green at the concentration of
0.04% in saturated picric acid solution for 30 min.

Why is important the pre-incubation?

Many thanks,
Fabio Grizzi

Dr. Fabio Grizzi
Direzione Scientifica, Istituto Clinico Humanitas
Via Manzoni 56
20089 Rozzano, Milan, Italy

Phone: +390282244548
Fax +390282244590
e-mail: fabio.grizzi-at-humanitas.it {mailto:fabio.grizzi-at-humanitas.it}





From daemon Thu Aug 3 12:57:48 2000



From: Philip Oshel :      peoshel-at-facstaff.wisc.edu
Date: Thu, 3 Aug 2000 12:45:23 -0500
Subject: Re: Polaroid Type 55 P/N film

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Curious. I've never heard this, but I won't disagree. It's
photodeveloper chemicals, same basic sort of stuff used in
photography darkrooms. I haven't seen any warnings about these
chemicals in the packaging materials/information sheets. It is common
sense to keep it off your hands, and to wash your hands if you get it
on them, but I haven't had any problems not getting the goop on my
hands. And I'd have several trophies for clumsiness if I hadn't
dropped and broken them all ...

Phil

} This message is in reply to Philip Oshel at U. of Wisconsin:
}
} I've always been told that the brown goop that is easy to get on your hands
} while pulling apart the photo & negative of the Polaroid T55 film is a known
} carcinogen and to wash your hands immediately upon contact.
}
} Jane L. LaGoy
} Development Engineer
} Bodycote IMT, Inc.
} 155 River Street
} Andover, MA 01810
} 978-470-1620
} jlagoy-at-bodycote-imt.com

--
}}}}}}}}}}}}}}}}}{{{{{{{{{{{{{{{{{
Philip Oshel
Supervisor, AMFSC and BBPIC
Dept. of Animal Health and Biomedical Sciences
University of Wisconsin
1656 Linden Drive
Madison, WI 53706-1581
voice: (608) 263-4162
fax: (608) 262-7420 (dept. fax)



From daemon Thu Aug 3 15:45:49 2000



From: David Bentley :      dlb-at-email.arizona.edu
Date: Thu, 03 Aug 2000 12:15:42 -0700
Subject: Re: Polaroid Type 55 P/N film

Contents Retrieved from Microscopy Listserver Archives
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The MSDS on the 55 developer states that the developer does not contain
any listed carcinogens by OSHA, IARC or NTP. The major danger seems to be
the corrosiveness of the alkali from Potassium and Lithium hydroxide.
Ingestion obviously is a danger due to these and other components.
Polaroid has it's MSDS's up on it's website at:

http://www.Polaroid.com/service/msds/index.html

Gloves and/or handwashing are very much in order.

} I've always been told that the brown goop that is easy to get on your hands
} while pulling apart the photo & negative of the Polaroid T55 film is a known
} carcinogen and to wash your hands immediately upon contact.




From daemon Thu Aug 3 16:49:08 2000



From: Djstrecker-at-ra.rockwell.com
Date: Thu, 3 Aug 2000 17:31:27 -0400
Subject: wireless lan interference

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Date sent: Wed, 02 Aug 2000 11:20:54 -0400
} From: "Shea Miller" {millers-at-em.agr.ca}
To: confocal-at-listserv.acsu.buffalo.edu,
microscopy-at-sparc5.microscopy.com


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Fellow microscopists,

Have any of you had any experience with the installation of a wireless lan
affecting the operation of your microscopes in any way? I would very much
appreciate your advice in this regard.

Sincerely,

Mick Thomas
-------------------------------------------------
Mick Thomas
UHV-STEM Laboratory
E-1 Clark Hall
Cornell University
Ithaca, NY 14853

Phone: 607-255-0650
Fax: 607-255-7658
e-mail: mgt3-at-ccmr.cornell.edu




From daemon Thu Aug 3 16:49:16 2000



From: Djstrecker-at-ra.rockwell.com
Date: Thu, 3 Aug 2000 17:31:28 -0400
Subject: Re: wireless lan interference

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I have not yet seen any effects, with the Apple Airport system installed
for my computers as well as a GigaHertz wireless broadcasting systems which
are
carrying video signals from one room to another.

The Computer monitors however are a different story.

Nestor



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} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From daemon Thu Aug 3 16:49:10 2000



From: Djstrecker-at-ra.rockwell.com
Date: Thu, 3 Aug 2000 17:31:23 -0400
Subject: Astromed CCD camera

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From daemon Thu Aug 3 16:49:20 2000



From: Djstrecker-at-ra.rockwell.com
Date: Thu, 3 Aug 2000 17:31:29 -0400
Subject: SEM/EDS COURSES.

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Hi , i wanted to know if anyone knew of any SEM/EDS courses in Mexico or
Texas.
Thanks.

Rafael Peña.




From daemon Thu Aug 3 16:49:21 2000



From: Djstrecker-at-ra.rockwell.com
Date: Thu, 3 Aug 2000 17:31:28 -0400
Subject: wireless lan interference

Contents Retrieved from Microscopy Listserver Archives
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Fellow microscopists,

Have any of you had any experience with the installation of a wireless lan
affecting the operation of your microscopes in any way? I would very much
appreciate your advice in this regard.

Sincerely,

Mick Thomas
-------------------------------------------------
Mick Thomas
UHV-STEM Laboratory
E-1 Clark Hall
Cornell University
Ithaca, NY 14853

Phone: 607-255-0650
Fax: 607-255-7658
e-mail: mgt3-at-ccmr.cornell.edu




From daemon Thu Aug 3 16:49:23 2000



From: Djstrecker-at-ra.rockwell.com
Date: Thu, 3 Aug 2000 17:31:22 -0400
Subject: Re: IC structure

Contents Retrieved from Microscopy Listserver Archives
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Well, I guess Mark and I would have to see a higher
magnification image, detailing the polysilicon, the
"birds beak" area, and the substrate. This way we
could tell whether this is a gate, with gate oxide, or
a diffusion contact. I am assuming that your cross
section is well into the middle area of the polysilicon
line, and not just at the edge.

Darrell




From daemon Thu Aug 3 16:49:24 2000



From: Djstrecker-at-ra.rockwell.com
Date: Thu, 3 Aug 2000 17:31:22 -0400
Subject: Astromed CCD camera

Contents Retrieved from Microscopy Listserver Archives
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From daemon Thu Aug 3 16:49:24 2000



From: Djstrecker-at-ra.rockwell.com
Date: Thu, 3 Aug 2000 17:31:29 -0400
Subject: SEM/EDS COURSES.

Contents Retrieved from Microscopy Listserver Archives
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Date sent: Wed, 02 Aug 2000 11:20:54 -0400
} From: "Shea Miller" {millers-at-em.agr.ca}
To: confocal-at-listserv.acsu.buffalo.edu,
microscopy-at-sparc5.microscopy.com


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Hi , i wanted to know if anyone knew of any SEM/EDS courses in Mexico or
Texas.
Thanks.

Rafael Peña.




From daemon Thu Aug 3 16:49:27 2000



From: Djstrecker-at-ra.rockwell.com
Date: Thu, 3 Aug 2000 17:31:28 -0400
Subject: Re: how to unsubscribe

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On Tue, 1 Aug 2000 19:09:28 -0400, Gen Pei wrote:

| ------------------------------------------------------------------------
| The Microscopy ListServer -- Sponsor: The Microscopy Society of America
| To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
| On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
| -----------------------------------------------------------------------.
|
|
| Thanks. Gen
| ******************************************************************
| Gen Pei
| Department of Materials Science and Engineering
| Cornell University
| 328 Thurston Hall
| tele: (607)255-5177
| fax:(607)255-2365
| gp35-at-cornell.edu
|
| ******************************************************************
|


Nelson Conti
164 Ferne Court
Palo Alto, CA 94306

Gen Pei-
I've unsubscribed myself one time. I believe that you need to send an
e-mail to the MSA Listserver (address = ? ), and just indicate
"unsubscribe"
in the subject heading. To find the address, go to the MSA Listserver Web
address, and it should be possible to find the e-mail address for the MSA
Listserver. Sorry, I don't recall it off-hand.
Good luck with unsubscribing.
Nelson Conti
P.S. When in doubt - you can also re-send your message, but
direct it to
Nestor Zaluzec. I'm sure that he can tell you precisely how to unsubscribe.






_______________________________________________________
Say Bye to Slow Internet!
http://www.home.com/xinbox/signup.html




From daemon Thu Aug 3 16:49:27 2000



From: Djstrecker-at-ra.rockwell.com
Date: Thu, 3 Aug 2000 17:31:29 -0400
Subject: GFP

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Hello everyone;
I have just started looking at a GFP-transformed fungus growing on plant
surfaces, using regular fluorescence microscopy. I can clearly see the
fungus using two filter sets: a UV set (ex/em 365/} 420) and a blue filter
set (ex/em 470/} 515). While the fluorescence in blue light is much
brighter, stuff that is blue/blue-white with UV illumination is green in
the blue light. is the "extra" green in the blue light likely to be where
the GFP is not as concentrated, and hence doesn't show up in the UV, or is
it more likely to be some other compound that has similar
excitation/emission characteristics? I have done a fair bit of reading on
the subject, but am still feeling a bit vague, and would really appreciate
hearing from those of you who have some first hand experience with GFP.

thanks in advance
shea



Dr. S.Shea Miller
Agriculture & AgriFood Canada
Eastern Cereal and Oilseed Research Centre
Rm. 2068 Neatby Building
Central Experimental Farm
Ottawa, Ontario
Canada K1A 0C6
Phone: (613) 759-1760
Fax: (613) 759-1701
E-mail: millers-at-em.agr.ca




From daemon Thu Aug 3 16:49:28 2000



From: Djstrecker-at-ra.rockwell.com
Date: Thu, 3 Aug 2000 17:31:29 -0400
Subject: GFP

Contents Retrieved from Microscopy Listserver Archives
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Hello everyone;
I have just started looking at a GFP-transformed fungus growing on plant
surfaces, using regular fluorescence microscopy. I can clearly see the
fungus using two filter sets: a UV set (ex/em 365/} 420) and a blue filter
set (ex/em 470/} 515). While the fluorescence in blue light is much
brighter, stuff that is blue/blue-white with UV illumination is green in
the blue light. is the "extra" green in the blue light likely to be where
the GFP is not as concentrated, and hence doesn't show up in the UV, or is
it more likely to be some other compound that has similar
excitation/emission characteristics? I have done a fair bit of reading on
the subject, but am still feeling a bit vague, and would really appreciate
hearing from those of you who have some first hand experience with GFP.

thanks in advance
shea



Dr. S.Shea Miller
Agriculture & AgriFood Canada
Eastern Cereal and Oilseed Research Centre
Rm. 2068 Neatby Building
Central Experimental Farm
Ottawa, Ontario
Canada K1A 0C6
Phone: (613) 759-1760
Fax: (613) 759-1701
E-mail: millers-at-em.agr.ca




From daemon Thu Aug 3 16:49:28 2000



From: Djstrecker-at-ra.rockwell.com
Date: Thu, 3 Aug 2000 17:31:23 -0400
Subject: CM-10 computer log-in

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Hi all,
We would like to add an individualized computer password system to our
FEI/Philips CM-10 TEM. We would like a user to be able to type in their
password which would then trigger a switch tied into the high tension and
turn the HT on. The system would ideally then have a timer to record "on"
time and a function to record this time with the user ID.
Connecting a timer to the high tension is not a problem...the computer
password part is where we need help.
Please contact me if you have a system such as this on a FEI/Philips
CM-10 or even on another make TEM which might work on a CM-10.
Thanks,
Debby

Debby Sherman, Manager Phone: 765-494-6666
Microscopy Center in Agriculture FAX: 765-494-5896
Dept. of Botany & Plant Pathology E-mail: sherman-at-btny.purdue.edu
Purdue University
1057 Whistler Building
West Lafayette, IN 47907-1057




From daemon Thu Aug 3 16:49:29 2000



From: Djstrecker-at-ra.rockwell.com
Date: Thu, 3 Aug 2000 17:31:28 -0400
Subject: Re: how to unsubscribe

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On Tue, 1 Aug 2000 19:09:28 -0400, Gen Pei wrote:

| ------------------------------------------------------------------------
| The Microscopy ListServer -- Sponsor: The Microscopy Society of America
| To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
| On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
| -----------------------------------------------------------------------.
|
|
| Thanks. Gen
| ******************************************************************
| Gen Pei
| Department of Materials Science and Engineering
| Cornell University
| 328 Thurston Hall
| tele: (607)255-5177
| fax:(607)255-2365
| gp35-at-cornell.edu
|
| ******************************************************************
|


Nelson Conti
164 Ferne Court
Palo Alto, CA 94306

Gen Pei-
I've unsubscribed myself one time. I believe that you need to send an
e-mail to the MSA Listserver (address = ? ), and just indicate
"unsubscribe"
in the subject heading. To find the address, go to the MSA Listserver Web
address, and it should be possible to find the e-mail address for the MSA
Listserver. Sorry, I don't recall it off-hand.
Good luck with unsubscribing.
Nelson Conti
P.S. When in doubt - you can also re-send your message, but
direct it to
Nestor Zaluzec. I'm sure that he can tell you precisely how to unsubscribe.






_______________________________________________________
Say Bye to Slow Internet!
http://www.home.com/xinbox/signup.html




From daemon Thu Aug 3 16:49:27 2000



From: Djstrecker-at-ra.rockwell.com
Date: Thu, 3 Aug 2000 17:31:28 -0400
Subject: Re: wireless lan interference

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I have not yet seen any effects, with the Apple Airport system installed
for my computers as well as a GigaHertz wireless broadcasting systems which
are
carrying video signals from one room to another.

The Computer monitors however are a different story.

Nestor



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From daemon Thu Aug 3 16:49:27 2000



From: Djstrecker-at-ra.rockwell.com
Date: Thu, 3 Aug 2000 17:31:29 -0400
Subject: Job For Electron Microscopist/Materials Engineer

Contents Retrieved from Microscopy Listserver Archives
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BOC Gases, has a unique opportunity for an Electron Microscopist/Materials
Engineer located at it's state of the
art Technical Center in Murray Hill, NJ.

BOC Gases, a division of The BOC Group Inc., was founded in 1888 and is a
global leader in the manufacturing
and distribution of industrial, medical, and specialty gases and related
equipment. Poised for new growth
opportunities, BOC currently operates in more than 50 countries, providing
customers with gases and technology
which are used to support life, freeze food, forge steel, shape glass,
refine petroleum, create computer chips, and
clean wastewater and smokestack emissions, in addition to hundreds of
other applications.

Electron Microscopist/Materials Engineer

Responsibilities

The successful candidate will support the materials characterization needs
of the Gases Technology R&D
organization using and maintaining analytical equipment, such as, Scanning
Electron Microscopy, X-ray Diffraction.
Metallography. Characterization includes metallic surfaces,
adsorption/catalysis materials as well as other materials
related to gases delivery equipment and vessels. This position is within
the analytical services part of the Materials
Technology Group. More specifically, the person will have the
responsibility to:

Provide scanning electron microscope (SEM), environmental scanning
electron microscope (ESEM), and
metallography services.

Maintain the SEM and ESEM equipment in good operating condition.

Develop new techniques to support researchers needs.

Perform failure analysis and provide technical assistance for material
selection projects.

Experience/Education

A B.S. or M.S. in the field of materials science and engineering with
practical working experience with
scanning electronic microscope techniques. Experience in x-ray diffraction
a plus.

A knowledge of a wide variety of materials. Experience with mechanical
testing, failure mechanisms,
corrosion, and materials, especially metals and polymers.
5-10 years work experience in a materials laboratory.

Work experience in the chemical processing industry a plus.

Ability to perform failure analysis.

Ability to multitask is essential.

Send application to: BOC Gases, Attn: Recruiting - SEM-NM, 575 Mountain
Avenue, Murray Hill, NJ
07974. E-mail: jobs-at-us.gases.boc.com


fax: 908-771-1702. Along with opportunities for professional growth
we offer competitive compensation and comprehensive benefits.




BOC Gases is an equal opportunity employer.


*********************************************************************
This footnote confirms that this e-mail message has been scanned for
the presence of known computer viruses by the MessageLabs Virus
Control Centre. However, it is still recommended that you use
local virus scanning software to monitor for the presence of viruses.
*********************************************************************




From daemon Thu Aug 3 16:49:28 2000



From: Djstrecker-at-ra.rockwell.com
Date: Thu, 3 Aug 2000 17:31:23 -0400
Subject: Astromed CCD camera

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Sorry for the previous blank message. Nestor thinks it was just a fluke.

We have an Astromed cooled CCD camera, which we would like to use.
However, we have no manuals for it. If you have manuals or other
information that would allow us to put this camera into operation please
let me know "off line".

Thank you,
Alwyn Eades



From daemon Thu Aug 3 16:49:28 2000



From: Djstrecker-at-ra.rockwell.com
Date: Thu, 3 Aug 2000 17:31:23 -0400
Subject: CM-10 computer log-in

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Hi all,
We would like to add an individualized computer password system to our
FEI/Philips CM-10 TEM. We would like a user to be able to type in their
password which would then trigger a switch tied into the high tension and
turn the HT on. The system would ideally then have a timer to record "on"
time and a function to record this time with the user ID.
Connecting a timer to the high tension is not a problem...the computer
password part is where we need help.
Please contact me if you have a system such as this on a FEI/Philips
CM-10 or even on another make TEM which might work on a CM-10.
Thanks,
Debby

Debby Sherman, Manager Phone: 765-494-6666
Microscopy Center in Agriculture FAX: 765-494-5896
Dept. of Botany & Plant Pathology E-mail: sherman-at-btny.purdue.edu
Purdue University
1057 Whistler Building
West Lafayette, IN 47907-1057




From daemon Thu Aug 3 16:49:29 2000



From: Djstrecker-at-ra.rockwell.com
Date: Thu, 3 Aug 2000 17:31:23 -0400
Subject: Astromed CCD camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


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Sorry for the previous blank message. Nestor thinks it was just a fluke.

We have an Astromed cooled CCD camera, which we would like to use.
However, we have no manuals for it. If you have manuals or other
information that would allow us to put this camera into operation please
let me know "off line".

Thank you,
Alwyn Eades



From daemon Thu Aug 3 16:49:29 2000



From: Djstrecker-at-ra.rockwell.com
Date: Thu, 3 Aug 2000 17:31:22 -0400
Subject: Re: IC structure

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Well, I guess Mark and I would have to see a higher
magnification image, detailing the polysilicon, the
"birds beak" area, and the substrate. This way we
could tell whether this is a gate, with gate oxide, or
a diffusion contact. I am assuming that your cross
section is well into the middle area of the polysilicon
line, and not just at the edge.

Darrell




From daemon Thu Aug 3 16:49:29 2000



From: Djstrecker-at-ra.rockwell.com
Date: Thu, 3 Aug 2000 17:31:27 -0400
Subject: wireless lan interference

Contents Retrieved from Microscopy Listserver Archives
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When I had resolution problems recently (which turned out to be due to a
"leaky" detector) one of the first things my service engineer asked me to
check was to make sure that the detector wasn't touching the pole piece
(which can cause a ground loop?) Try backing the detector out a little to
see if the peak width changes. I doubt it will help, but it is easy to
try.
Matt




"E. J. McKenzie" {elizm-at-pdx.edu} on 07/28/2000 12:30:59 PM





To: microscopy-at-sparc5.microscopy.com
cc:


Hi everyone,

we are having serious problems with the resolution of our EDS detector -
the
peak height-width ratio is terrible, around 450 counts. Consequently, our
peaks look more like rolling hills and one peak blurs into another. I have
callibrated the machine using an Al-Cu standard, checked that the detector
is kept cool, sample is tilted at 30 degrees, etc etc. I also got another
microscopist to come and have a look to check my methods and he also
concluded there was a serious problem.

Is there anything else I could try before I phone the Noran technicians and
pay them a lot of $$$ to fix/replace the detector?

regards
Liz McKenzie
--------------------------------------------------
Geomicrobiology and Electron Microscopy Laboratory
Room S9 Cramer Hall
1721 SW Broadway
Portland State University
Portland
OR97201

ph:503 725 3362
fax:503 725 3025



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I'm trying to find references for vital staining with fluorobora stains (or
fluorabora). There don't seem to be any, so I don't know if this is because they
don't work, or because they just didn't catch on. Has anyone any experience of
using them, and could anyone give me any references? Thanks.

Lesley Weston.





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Hi , i wanted to know if anyone knew of any SEM/EDS courses in Mexico or
Texas.
Thanks.

Rafael Peña.



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Well, I guess Mark and I would have to see a higher
magnification image, detailing the polysilicon, the
"birds beak" area, and the substrate. This way we
could tell whether this is a gate, with gate oxide, or
a diffusion contact. I am assuming that your cross
section is well into the middle area of the polysilicon
line, and not just at the edge.

Darrell



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Fellow microscopists,

Have any of you had any experience with the installation of a wireless lan
affecting the operation of your microscopes in any way? I would very much
appreciate your advice in this regard.

Sincerely,

Mick Thomas
-------------------------------------------------
Mick Thomas
UHV-STEM Laboratory
E-1 Clark Hall
Cornell University
Ithaca, NY 14853

Phone: 607-255-0650
Fax: 607-255-7658
e-mail: mgt3-at-ccmr.cornell.edu




From daemon Thu Aug 3 18:29:23 2000



From: Purdy, Sam :      SPurdy-at-nationalsteel.com
Date: Thu, 3 Aug 2000 18:19:51 -0500
Subject: Nikon Coolpix 900

Contents Retrieved from Microscopy Listserver Archives
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Jonathon:

Contact Diagnostic Instruments, www.diaginc.com or info-at-diaginc.com.
They manufacture couplings to connect cameras and microscopes.

Sam Purdy
Staff Specialist
National Steel Corp.





From daemon Thu Aug 3 18:38:26 2000



From: Lesley Weston :      lesley-at-interchange.ubc.ca
Date: Thu, 3 Aug 2000 14:33:53 -0700 (PDT)
Subject: Fluorobora

Contents Retrieved from Microscopy Listserver Archives
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I am currently being tasked to bring my Amray 3200C SEM into compliance with
ISO9000. A major issue with my supervision is the accuracy of the micron
bar given with every picture - and what proceedure do I use for
callibration. If you have any insight or suggestions, I would appreciate
it.
Thanks
Gary Weaver
Gary.Weaver-at-MW.Boeing.com




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Hi all,
We would like to add an individualized computer password system to our
FEI/Philips CM-10 TEM. We would like a user to be able to type in their
password which would then trigger a switch tied into the high tension and
turn the HT on. The system would ideally then have a timer to record "on"
time and a function to record this time with the user ID.
Connecting a timer to the high tension is not a problem...the computer
password part is where we need help.
Please contact me if you have a system such as this on a FEI/Philips
CM-10 or even on another make TEM which might work on a CM-10.
Thanks,
Debby

Debby Sherman, Manager Phone: 765-494-6666
Microscopy Center in Agriculture FAX: 765-494-5896
Dept. of Botany & Plant Pathology E-mail: sherman-at-btny.purdue.edu
Purdue University
1057 Whistler Building
West Lafayette, IN 47907-1057



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Hello everyone;
I have just started looking at a GFP-transformed fungus growing on plant
surfaces, using regular fluorescence microscopy. I can clearly see the
fungus using two filter sets: a UV set (ex/em 365/} 420) and a blue filter
set (ex/em 470/} 515). While the fluorescence in blue light is much
brighter, stuff that is blue/blue-white with UV illumination is green in
the blue light. is the "extra" green in the blue light likely to be where
the GFP is not as concentrated, and hence doesn't show up in the UV, or is
it more likely to be some other compound that has similar
excitation/emission characteristics? I have done a fair bit of reading on
the subject, but am still feeling a bit vague, and would really appreciate
hearing from those of you who have some first hand experience with GFP.

thanks in advance
shea



Dr. S.Shea Miller
Agriculture & AgriFood Canada
Eastern Cereal and Oilseed Research Centre
Rm. 2068 Neatby Building
Central Experimental Farm
Ottawa, Ontario
Canada K1A 0C6
Phone: (613) 759-1760
Fax: (613) 759-1701
E-mail: millers-at-em.agr.ca



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Date sent: Wed, 02 Aug 2000 11:20:54 -0400
} From: "Shea Miller" {millers-at-em.agr.ca}
To: confocal-at-listserv.acsu.buffalo.edu,
microscopy-at-sparc5.microscopy.com


I'm trying to find references for vital staining with fluorobora stains (or
fluorabora). There don't seem to be any, so I don't know if this is because they
don't work, or because they just didn't catch on. Has anyone any experience of
using them, and could anyone give me any references? Thanks.

Lesley Weston.






From daemon Thu Aug 3 19:35:20 2000



From: Djstrecker-at-ra.rockwell.com
Date: Thu, 3 Aug 2000 17:31:22 -0400
Subject: Phys Sample Prep Super Glues

Contents Retrieved from Microscopy Listserver Archives
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You will still need Nikon Part# 82014 (Optical Coupler 28mm to C-Mount)
available only from your local Nikon Microscope Dealer.

Regards,

Lawrence Kordon
Nikon, Inc.
nikon-at-jagunet.com

"Purdy, Sam" wrote:

} ------------------------------------------------------------------------
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} -----------------------------------------------------------------------.
}
} Jonathon:
}
} Contact Diagnostic Instruments, www.diaginc.com or info-at-diaginc.com.
} They manufacture couplings to connect cameras and microscopes.
}
} Sam Purdy
} Staff Specialist
} National Steel Corp.



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With regard to the posting I made on this subject, Chuck Garber sent me the
following note wherein he raises a number of important points. Chuck is
correct in all regards. The product we were using was not DURO. We have
no experience with Duro. We have made specific, brand name,
recommendations in the past and my note was to share our recent experiences
regarding these products. The products we were using were not "garage
level" products.

So, IF, and only IF, you are using a product solely because of a
recommendation from our lab, we now make new recommendations.

Ron

-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Hi Ron,

I guess I should give some kind of disclaimer statement first, that in no
way am I suggesting that what you are saying it not correct. If this was
your perceived experience, then I am sure it is correct and I am not
questioning it.

And it is a fact that there are some garage level operations where people
take from 5 gallon pales and "fill" the 1 oz plastic bottles and put on a
label and put it into distribution, without any real testing of the
integrity of their packaging or any of the other things you and I might
think one should be doing to insure the integrity of their product.

But I can assure you, without hesitation, the cyanoacrylate that is made by
Duro, which is indeed a consumer products company, but it is also "top in
class" when it comes to this type of product, has been offered by us (e.g.
SPI) for years and we have never ever had anyone every voice even the
slight
complaint about it. We also decided a long time ago that once opened, the
shelf life of these products can be very short, and consequently, we
decided
to offer the product in the smaller 3g package.

I go through the trouble to point this out to you because I see things from
a slightly different perspective possibly than you do:

1] For one thing, someone even at IBM, having to place an order with
either
of the two mentioned companies, given the cost of placing an order period
of
any size, what is the overhead going to be? But if someone was ordering
the
Duro product from someone like SPI, then it just gets added in with a bunch
of other things and the real cost is almost nothing.

2] For someone in Mexico, the situation is far worse. You have a lot of
influence on people because of your reputation. Your posting could make
someone in Mexico City think that in order to get acceptable results, they
have to place a direct order in the USA for one of these "special"
cyanoacrylates and in situations with limited research budgets, it just
seems to me like they have better ways to be spending their money.


So while the Duro product might not be any better than the two mentioned,
the point is that because of the distribution and transportation costs, if
people are led to believe that they really do need something that can not
be
procured from their traditional sources of purchasing, they end up wasting
a
lot of money unnecessarily.

Of course if you had any hard evidence that the Duro product was in some
way
inferior, that would surely be another story. But chemically, these are
all
the same identical materials, they all have the same identical MSDS
information and the all perform identically. When ever I have heard of
someone having problems it has been because they were using product from a
previously opened package.

Feel free to use this information however you feel might be approriate,
either in whole or in part. But I did want to share my views with you on
your posting, which I hope you won't mind my having done.......

Chuck



Ron Anderson, IBM, Hopewell Jct., New York, USA. anderron-at-us.ibm.com

IBM Analytical Services; http://www.chips.ibm.co



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With regard to the posting I made on this subject, Chuck Garber sent me the
following note wherein he raises a number of important points. Chuck is
correct in all regards. The product we were using was not DURO. We have
no experience with Duro. We have made specific, brand name,
recommendations in the past and my note was to share our recent experiences
regarding these products. The products we were using were not "garage
level" products.

So, IF, and only IF, you are using a product solely because of a
recommendation from our lab, we now make new recommendations.

Ron

-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Hi Ron,

I guess I should give some kind of disclaimer statement first, that in no
way am I suggesting that what you are saying it not correct. If this was
your perceived experience, then I am sure it is correct and I am not
questioning it.

And it is a fact that there are some garage level operations where people
take from 5 gallon pales and "fill" the 1 oz plastic bottles and put on a
label and put it into distribution, without any real testing of the
integrity of their packaging or any of the other things you and I might
think one should be doing to insure the integrity of their product.

But I can assure you, without hesitation, the cyanoacrylate that is made by
Duro, which is indeed a consumer products company, but it is also "top in
class" when it comes to this type of product, has been offered by us (e.g.
SPI) for years and we have never ever had anyone every voice even the
slight
complaint about it. We also decided a long time ago that once opened, the
shelf life of these products can be very short, and consequently, we
decided
to offer the product in the smaller 3g package.

I go through the trouble to point this out to you because I see things from
a slightly different perspective possibly than you do:

1] For one thing, someone even at IBM, having to place an order with
either
of the two mentioned companies, given the cost of placing an order period
of
any size, what is the overhead going to be? But if someone was ordering
the
Duro product from someone like SPI, then it just gets added in with a bunch
of other things and the real cost is almost nothing.

2] For someone in Mexico, the situation is far worse. You have a lot of
influence on people because of your reputation. Your posting could make
someone in Mexico City think that in order to get acceptable results, they
have to place a direct order in the USA for one of these "special"
cyanoacrylates and in situations with limited research budgets, it just
seems to me like they have better ways to be spending their money.


So while the Duro product might not be any better than the two mentioned,
the point is that because of the distribution and transportation costs, if
people are led to believe that they really do need something that can not
be
procured from their traditional sources of purchasing, they end up wasting
a
lot of money unnecessarily.

Of course if you had any hard evidence that the Duro product was in some
way
inferior, that would surely be another story. But chemically, these are
all
the same identical materials, they all have the same identical MSDS
information and the all perform identically. When ever I have heard of
someone having problems it has been because they were using product from a
previously opened package.

Feel free to use this information however you feel might be approriate,
either in whole or in part. But I did want to share my views with you on
your posting, which I hope you won't mind my having done.......

Chuck



Ron Anderson, IBM, Hopewell Jct., New York, USA. anderron-at-us.ibm.com

IBM Analytical Services; http://www.chips.ibm.co




From daemon Fri Aug 4 00:35:00 2000



From: ralelo96-at-latino.net.co (Larry)
Date: Fri, 4 Aug 2000 00:31:28 -0500 (CDT)
Subject: No one is turned down

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From daemon Fri Aug 4 08:38:10 2000



From: ABM Siddique :      siddiqueabm-at-hotmail.com
Date: Fri, 4 Aug 2000 08:21:33 -0500
Subject: TEM of starch granules

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Dear All:

I have been trying to study the ultra-structure (TEM) of strach granules
(in wheat and maize seeds) that contains amorphus and crystaline layers.
Unfortunately, I am unable to look at these layers under TEM even after
keeping the tissues longer time in fixative (over night in
3%gluteraldehyde) and embedding medium (LR white/Spurs for 2 days).

I also tried the the above procedure with isolated starch granules in low
melted agar but the result is the same - poor penetration of embedding
resin as a result I am getting broken starch granules without any
ultrastructural details.

I would appreciate it if anybody could suggest me how to obtaine a
reasonably good ultra-structural (TEM) details of starch granules.

With thanks,

Siddique


Get Your Private, Free E-mail from MSN Hotmail at
{http://www.hotmail.com/} http://www.hotmail.com






From daemon Fri Aug 4 09:41:09 2000



From: Debby Sherman :      sherman-at-btny.purdue.edu
Date: 04 Aug 2000 09:28:58 -0500
Subject: Facility Management session

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Below is the program for the Facility Management session at M&M 2000. I am trying to record the sessions so we can summarize as much of the discussion as possible and make it available through the list for those of you who cannot attend. For those of you that can attend, please bring any informationin the form of handouts that you feel helpful in discussion of the topics. I would also appreciate this information sent to me as an attachment so that it can be added to the final summation of the session.
Hope to see many of you in Philadelphia.
Debby Sherman

Debby Sherman, Manager Phone: 765-494-6666
Microscopy Center in Agriculture FAX: 765-494-5896
Dept. of Botany & Plant Pathology E-mail: sherman-at-btny.purdue.edu
Purdue University
1057 Whistler Building
West Lafayette, IN 47907-1057

--------

Experts Session on Facility Management

Wednesday, Aug. 16
9:00AM to Noon
Room 106

The following topics will each be briefly introduced by a presenter followed by an open discussion period.

*9:00am: Multi-user Facilities…Managing Users
Keith Darling, Ph.D.
Manager, Analytical Research Dept, Atofina Chemicals, Inc.

*9:45am: Justification of costs/cost recovery….Electronic bookkeeping and billing.
Gregory W. Erdos, Ph.D.
Ass’t Director, U. of Florida Biotechnology Program
Scientific Director, Electron Microscopy Core Lab

Break

*10:40am: Equipment Maintenance: Vender service contracts vs. Independent providers vs. Insurance company/self-insure options
Paul J. Wirtz
President, Expense Reduction Analysts International

*Times approximate…Additional discussion starting at 11:30am.





From daemon Fri Aug 4 11:04:30 2000



From: Kriho, Virginia :      Vkriho-at-psych.uic.edu
Date: Fri, 4 Aug 2000 10:53:54 -0500
Subject: FW: Birdcage

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Message-ID: {B98B03BD7E44D411ACE300D0B773E8CA442456-at-mail.psych.uic.edu}
"'Dick Burry'" {burry.1-at-osu.edu} ,
"'Don Theyken'" {dtheyken-at-mindspring.com} ,
"'Dr. A. J. Tousimis'" {trc-at-tousimis.com} ,
"'Dr. Ed Daniels'"
{ewdaniels-at-earthlink.net} ,
"'Dr. Hector Caruncho'" {cmhector-at-usc.es} ,
"'Dr. Hsi-Yuan Yang'" {hyhy-at-ccms.ntu.edu.tw} ,
"'Dr. Jaci Sagen'"
{jsagen-at-miamiproject.med.miami.edu} ,
"'Dr. Marietta Issidorides'"
{mrissid-at-compulink.gr} ,
"'Dr. P. Chien'" {p-chien-at-nwu.edu} ,
"'Dr. Sookja Chung'" {skchung-at-hkucc.hku.hk} ,
"'John Kriho'"
{jk-at-meadowsinfo.com} ,
"'John Robinson'" {robinson.21-at-osu.edu} ,
"'Kathy Wolken'" {wolken.1-at-osu.edu} , "'Laura Kriho'" {lkriho-at-ucar.edu} ,
"'Lily Yang'" {lsyang77-at-cm1.hinet.net} ,
"'Lisa Adler'"
{horizonco-at-mindspring.com} ,
"'List Server'"
{microscopy-at-sparc5.microscopy.com} ,
"'Murat Elcin'"
{elcin-at-science.ankara.edu.tr} ,
"'Nicholas Kriho'" {idklaus1-at-uic.edu} ,
"'Ralph J. Kriho'" {rjkriho-at-hotmail.com}




} ----------
} From: Hasemeier, Cynthia
} Sent: Friday, August 04, 2000 10:39 AM
} To: beth bourland; Fran Layne; Ginny; Harry; JDB; Judy Sobiesk; Kathleen
} McMullan; marksr; mikedeb; Pauline; Ron; Ted; Fitzgibbon, Genevieve;
} Madison, Sybil; Manning, George; Montgomery, John; Kriho, Virginia; Mason,
} Sally; Peterson, Jim; Rodriguez, Walter
} Subject: Birdcage
}
} Subject: THE BIRDCAGE
}
} There once was a man named George Thomas, a pastor in a small New England
} town. One Easter Sunday morning he came to the Church carrying a rusty,
} bent, old birdcage, and set it by the pulpit. Several eyebrows were raised
} and, as if in response, Pastor Thomas began to speak. "I was walking
} through town yesterday when I saw a young boy coming toward me swinging
} this bird cage. On the bottom of the cage were three little wild birds,
} shivering with cold and fright.
}
} I stopped the lad and asked, 'What you got there son?'
}
} "Just some old birds," came the reply.
}
} "What are you gonna do with them?" I asked.
}
} "Take 'em home and have fun with 'em," he answered. I'm gonna tease 'em
} and pull out their feathers to make 'em fight. I'm gonna have a real good
} time."
}
} "But you'll get tired of those birds sooner or later. What will you do?"
}
} "Oh,! I got some cats," said the little boy. "They like birds. I'll take
} 'em to them."
}
} The pastor was silent for a moment. "How much do you want for those birds,
} son?"
}
} "Huh?!!! Why, you don't want them birds, mister. They're just plain old
} field birds. They don't sing - they ain't even pretty!"
}
} "How much?" the pastor asked again.
}
} The boy sized up the pastor as if he were crazy and said, "$10?" The
} pastor reached in his pocket and took out a ten-dollar bill. He placed it
} in the boy's hand. In a flash, the boy was gone.
}
} The pastor picked up the cage and gently carried it to the end of the
} alley where there was a tree and a grassy spot. Setting the cage down, he
} opened the door, and by softly tapping the bars persuaded the birds out,
} setting them free. Well, that explained the empty birdcage on the pulpit,
} and then the pastor began to tell this story*
}
} One day Satan and Jesus were having a conversation. Satan had just come
} from the Garden of Eden, and he was gloating and boasting. "Yes, sir, I
} just caught the world full of people down there. Set me a trap, used bait
} I knew they couldn't resist. Got 'em all!"
}
} "What are you going to do with them?" Jesus asked.
}
} Satan replied, "Oh, I'm gonna have fun! I'm gonna teach them how to marry
} and divorce each other, how to hate and abuse each other, how to drink and
} smoke and curse. I'm gonna teach them how to invent guns and bombs and
} kill each other. I'm really gonna have fun!"
}
} "And what will you do when you get done with them?" Jesus asked.
}
} "Oh, I'll kill 'em," Satan glared proudly.
}
} "How much do you want for them?" Jesus asked.
}
} "Oh, you don't want those people. They ain't no good. Why, you'll take
} them and they'll just hate you. They'll spit on you, curse you and kill
} you! You don't want those people!!"
}
} "How much?" He asked again.
}
} Satan looked at Jesus and sneered, "All your tears, and all your blood."
}
} Jesus said, "DONE!" Then He paid the price.
}
} The pastor picked up the cage he opened the door and he walked from the
} pulpit. Isn't it funny how simple it is for people to trash God and then
} wonder why the world's going to hell. Isn't it funny how someone can say
} "I believe in God" but still follow Satan (who, by the way, also
} "believes" in God).
}
} Isn't it funny how you can send a thousand jokes through e-mail and they
} spread like wildfire, but when you start sending messages regarding the
} Lord, people think twice about sharing. Isn't it funny how when you go to
} forward this message, you will not send it to many on your
} address list because you're not sure what they believe, or what they will
} think of you for sending it to them. Isn't it funny how I can be more
} worried about what other people think of me than what God thinks of me?
}
} I pray, for everyone who sends this to their entire address book, they
} will be blessed by God in a way special for them.
}
}
}
}
}
}
} ------------------------------------------------------------
} Cindy Hasemeier, M.A.
} IRB Coordinator, Department of Psychiatry
} University of Illinois at Chicago
} PI 328W, M/C 912
} 312.413.4563
} 312.413.4544 (fax)
}
}



From daemon Fri Aug 4 11:12:32 2000



From: Helvey, Marc :      Marc.Helvey-at-vlsistd.com
Date: Fri, 4 Aug 2000 09:00:32 -0700
Subject: ISO9000 requirements

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Hi Gary -

I know Nestor wants to keep the gratuitous solicitations to a minimum, but
VLSI Standards might have a valid, NIST Traceable, ISO compliant solution
for you. If need be, I also have some contacts at Amray (KLA-Tencor) that
might be able to assist you. Please feel free to contact me directly
offline.

Regards -

Marc Helvey
VLSI Standards, Inc.
3087 North First Street
San Jose, CA 95134-2006
Phone: (408) 428-1800, ext. 108
FAX: (408) 428-9555
Mobile: (408) 307-3833
E-Mail: marc.helvey-at-vlsistd.com {mailto:marc.helvey-at-vlsistd.com}
Internet: http://www.vlsistd.com
 
 


-----Original Message-----
} From: Weaver, Gary E [mailto:Gary.Weaver-at-MW.Boeing.com]
Sent: Thursday, August 03, 2000 4:29 PM
To: Microscopy-at-sparc5.microscopy.com


I am currently being tasked to bring my Amray 3200C SEM into compliance with
ISO9000. A major issue with my supervision is the accuracy of the micron
bar given with every picture - and what proceedure do I use for
callibration. If you have any insight or suggestions, I would appreciate
it.
Thanks
Gary Weaver
Gary.Weaver-at-MW.Boeing.com





From daemon Fri Aug 4 13:17:01 2000



From: Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 04 Aug 2000 10:56:11 -0700
Subject: IC Structure

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Again, thanks to all who have contributed ideas about
this IC cross section. I think that I have it sorted out.
Maybe 95% right (??). The following URL shows an
annotated cross section image. The poly gate is
in the central portion of the image above the
substrate. I was not sure if lettering would fit or show up.

http://photoweb.net/icxsect3.htm

I have not been able to confirm or deny that the gate
truly is poly. My new x-ray system won't be available
for several months. It should be a Rontec UHV 133eV
system. Some responses indicated that x-ray would
work in analyzing the cross sectioned gate. Other
responses said it would not. I guess that the proof
is in the trying. I suppose that there is some limit
in how small the gate is where x-ray will not work.
This would be due to either confusion of the
results due to x-ray dispersion in a small volume
and/or limitations of spot size.

Again, thanks for the great responses. Stay tuned
as more info is gained. Further work is getting
under way for 0.6u EEPROM analysis. This
construction uses TiW plugs.

gary g.




From daemon Fri Aug 4 13:55:28 2000



From: Kriho, Virginia :      Vkriho-at-psych.uic.edu (by way of Nestor J.
Date: Fri, 4 Aug 2000 13:45:09 -0500
Subject: Apology.... Bird Cage

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Dear Listserver members,
Sending the Bird Cage to the listserver was a mistake. I thought I
was sending to a different listserver. I apologize to all. I will never
do this again, but I still cannot believe the furor. Once again I
apologize
to all. VirginiaKriho





From daemon Fri Aug 4 15:31:06 2000



From: John Foust :      jfoust-at-threedee.com
Date: Fri, 04 Aug 2000 14:45:41 -0500
Subject: Help identify old Zeiss scope

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I picked up a big old Zeiss microscope. Care to help me
understand what I have? Please send hints in private e-mail
and I'll summarize later. My description is at:

Is it a fluoro-phase scope?

- John




From daemon Fri Aug 4 16:13:01 2000



From: John Foust :      jfoust-at-threedee.com
Date: Fri, 04 Aug 2000 16:01:28 -0500
Subject: Help identify old Zeiss scope

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I picked up a big old Zeiss microscope. Care to help me
understand what I have? Please send hints in private e-mail
and I'll summarize later. My description is at:

http://www.threedee.com/zeiss/

Is it a fluoro-phase scope?

- John




From daemon Fri Aug 4 17:02:35 2000



From: milesd-at-us.ibm.com
Date: Fri, 4 Aug 2000 17:50:02 -0400
Subject: Re: IC Structure

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Gary,

Your suggested process flow is a little different from all I am used to.
First, what etches has this cross section seen? I would venture a
guess that the gate is polysilicon, the layer that forms around the poly
gate is a CVD (chemical vapor [or is that vapour] deposition) oxide.
The next layer is a softer glass deposited and then re-flowed (or
spun on). The next layer (marked poly Si), I would guess to be
aluminum. The next layer (marked Al), I would guess to be CVD
oxide, and the layer on top of that, (marked PSG) might be oxide or
silicon nitride.

Mind you, these are all just guesses. In my small world, I have never
seen aluminum deposited directly on polysilicon. (That does not
mean that it is never done) Aluminum usually shows some texture in
a decorated cross section. It is not usually a smooth surface. That
level has the appearance I am accustomed to seeing on oxide
layers (both gray tones and surface). Most processes define well
regions, grow the field oxide, grow the gate oxide, put on the gates,
implant source/drains (self aligned to the gates), and then build up
from there. The interlevel dielectrics must be deposited, they cannot
be grown, like the gate oxide and the field oxide.

I looked through my books, and found a couple with basic process
descriptions:

Physical Design of CMOS Integrated Circuits
using L-EDIT
by John Uymura
ISBN 0-534-94326-8
PWS Publishing Co.
20 Park Plaza
Boston, MA 02116-4324

Microelectronic Processing
An Introduction to the Manufacture of Integrated
Circuits
by W. Scot Ruska
ISBN 0-07-054280-5
McGraw-Hill Book Company

[No financial connections with these books. I have taken
several continuing education classes with John, and we
have become friends from this long association]

Darrell




From daemon Fri Aug 4 18:29:56 2000



From: Giles, Bill :      William.Giles-at-TIMET.com
Date: Fri, 4 Aug 2000 18:46:15 -0500
Subject: T55 Developer "NOT" carcinogenic according to Polaroid

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This was a very good parable. Bill Armor, Laverne, OK
----- Original Message -----
} From: Kriho, Virginia {Vkriho-at-psych.uic.edu}
To: 'Charles Meshul' {meshulc-at-ohsu.edu} ; 'Dave Yeomans' {yeomans-at-uic.edu} ;
'Dick Burry' {burry.1-at-osu.edu} ; 'Don Theyken' {dtheyken-at-mindspring.com} ;
'Dr. A. J. Tousimis' {trc-at-tousimis.com} ; 'Dr. Ed Daniels'
{ewdaniels-at-earthlink.net} ; 'Dr. Hector Caruncho' {cmhector-at-usc.es} ; 'Dr.
Hsi-Yuan Yang' {hyhy-at-ccms.ntu.edu.tw} ; 'Dr. Jaci Sagen'
{jsagen-at-miamiproject.med.miami.edu} ; 'Dr. Marietta Issidorides'
{mrissid-at-compulink.gr} ; 'Dr. P. Chien' {p-chien-at-nwu.edu} ; 'Dr. Sookja Chung'
{skchung-at-hkucc.hku.hk} ; 'John Kriho' {jk-at-meadowsinfo.com} ; 'John Robinson'
{robinson.21-at-osu.edu} ; 'Kathy Wolken' {wolken.1-at-osu.edu} ; 'Laura Kriho'
{lkriho-at-ucar.edu} ; 'Lily Yang' {lsyang77-at-cm1.hinet.net} ; 'Lisa Adler'
{horizonco-at-mindspring.com} ; 'List Server'
{microscopy-at-sparc5.microscopy.com} ; 'Murat Elcin'
{elcin-at-science.ankara.edu.tr} ; 'Nicholas Kriho' {idklaus1-at-uic.edu} ; 'Ralph
J. Kriho' {rjkriho-at-hotmail.
Sent: Friday, August 04, 2000 10:53 AM


Suprised some one from Polaroid or one of their distributors has not
responded to this.

} I've always been told that the brown goop that is easy to get on your hands
} while pulling apart the photo & negative of the Polaroid T55 film is a
known
} carcinogen and to wash your hands immediately upon contact.

According to Polaroid's MSDS
(http://www.polaroid.com/service/msds/index.html) the developer in the pods
of T55 is an "alkali".

If you look on page 4 of the MSDS you will see the following:

"Does not contain any chemicals listed as carcinogens by OSHA, IARC or NTP"

Guess you'll have to believe them or not.

I've gone to T53, will see how it compares.

The opinions expressed above are my own, blah blah blah yackity smackity.

Bill





From daemon Fri Aug 4 19:49:30 2000



From: Ken Converse :      qualityimages-at-netrax.net
Date: Fri, 04 Aug 2000 19:42:18 -0700
Subject: Re: SE: JEOL 840/63xx noisy roughing pump

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michael shaffer wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Our roughing pump which service our JSM6300 has began making a
} rumbling noise which I might believe is bearings ... altho it could be
} the pump's bearings or the motor's. Has anyone serviced this problem?
}
} tia and cheerios, =shAf= :o)
}
} {} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {}
} Michael Shaffer, R.A. - mshaf-at-darkwing.uoregon.edu
} Geological Science's Electron Probe Facility - University of Oregon
} http://epmalab.uoregon.edu/
shAf,
I once got a Welsh 1402 for free that was making a terrible knocking
noise (also belt drive). When I rebuilt it , I found a alightly swollen
phenolic vane that was sticking. A little sanding and a gasket kit and
it was as good as new. An off-the-wall problem, but a possibility if
the JEOL pump has other than metal vanes.

Ken Converse
Quality Images
third party SEM service
Delta, PA





From daemon Fri Aug 4 21:40:35 2000



From: Terje Dokland :      dokland-at-ima.org.sg
Date: Sat, 5 Aug 2000 10:27:46 +0800
Subject: EM technician position at IMA, Singapore

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ELECTRON MICROSCOPIST
Laboratory of Structural Biology
Institute of Molecular Agrobiology, Singapore

A position is available for an experienced EM technician at the
Institute of Molecular Agrobiology (IMA), Singapore, in the group of Dr.
Terje Dokland, Laboratory of Structural Biology. The successful candidate
will be involved in several research projects, in two broad areas: (1)
Structural characterization of viruses by cryo-EM and 3D reconstruction;
(2) Study of virus assembly processes in vivo through freeze-substitution,
thin sectioning and immuno-cytochemistry. Experience with one or more of
these techniques would be advantageous.
IMA is a well funded and rapidly expanding research institute which
was established in 1995 to conduct basic and applied research in
agrobiology. It is located in a modern, spacious and well-equipped building
at the National University of Singapore campus. The EM lab presently has a
100kV TEM equipped with a Gatan cryo-stage and a cryo-SEM and a new 200kV
LaB6 TEM dedicated to structural microscopy will be purchased shortly.
Further information about the institute can be found at
http://www.ima.org.sg. Singapore is a cosmopolitan and multicultural
society offering all the conveniences of a modern city, and is centrally
located in Southeast Asia with easy access to other countries in the
region.
The position will be filled at the Research Officer or Assistant
Research Officer level, depending on experience, and the contract will be
initially offered for two years. IMA offers attractive salaries with bonus
packages and benefits.
Informal enquiries are welcome and can be directed to Terje Dokland
at dokland-at-ima.org.sg. Applications, including a CV and names of two
referees, should be sent to
Terje Dokland
Institute of Molecular Agrobiology
1 Research Link
The National University of Singapore
Singapore 117604

------------------------------------
Terje Dokland
Senior Scientist
Institute of Molecular Agrobiology
1 Research Link
The National University of Singapore
Singapore 117604
Phone: 65-872 7405 Fax: 65-872 7007
E-mail: dokland-at-ima.org.sg

"Convictions are greater enemies of truth than lies"
F. Nietzsche





From daemon Sat Aug 5 08:43:45 2000



From: Mansour A. Al-Shafei :      shafeima-at-aramco.com.sa
Date: Sat, 5 Aug 2000 08:30:52 -0500
Subject: M&M2000: looking to visit labs and research in Oil industry

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I will be attending M&M2000 and I am looking to visit labs and research
centers that are utilizing SEM, ESEM and AFM in their studies in the fields
of oil industry such as oil well cement, catalyst, corrosion and concrete.
Any names, addresses will be highly appreciated.
Thanks
Mansour Al-Shafei
Senior Lab Sci.
Saudi Aramco Oil Company
Lab Research & Development Center
Analytical Sciences Division
Advanced Instruments Unit
Voice: 966-3-876-4360
E-mail: shafeima-at-aramco.com.sa





From daemon Sat Aug 5 23:33:08 2000



From: Damian :      dneuberger-at-mindspring.com
Date: Sat, 05 Aug 2000 23:09:27 -0500
Subject: LM plastic microtome sections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Good evening,

I have been asked to help a colleague with a problem he is having cutting
good sections for light microscopy. He has a Reichert-Jung 2030 microtome
and a new tungsten carbide microtome knife (I do not know the exact type of
knife). He is trying to cut ABS with a R scale value of 105 and
polycarbonate with a M scale value of 70 (R-value of ~120 according to him).

Every section he has made exhibits numerous scratch marks that are also
evident in the block face. I briefly tried to face the block and also got
scratch marks. I cleaned the knife edge, tried different cutting angles
and speeds (manual only). I have cut this material to make good sections
and block face but I used an ultramicrotome and diamond knife. But to do
this for all his work is not an option.

Any suggestions as to how the sections and the block face can be improved?

Thanks so much in advance,


Damian Neuberger, Ph.D.
Baxter Healthcare, Inc.
WG3-2S
P.O. Box 490
Round Lake, IL 60073
e-mail: damian_neuberger-at-baxter.co,
Tel: 847-270-5888
Fax: 847-270-5897




From daemon Mon Aug 7 07:55:14 2000



From: Margaret Brannigan :      brannign-at-asrr.arsusda.gov
Date: Mon, 7 Aug 2000 08:32:32 -0400
Subject: TEM: yellow PbCitrate

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

Does anyone know why freshly made lead citrate sometimes comes out
yellow, and what adverse effects, if any, result? In going over
past threads on the subject, it seems most people have a relatively
colorless product as the end result, but a few report the yellow
color as normal. After years of making up "colorless" lead citrate,
mine is now yellow. I've toyed with NaOH sources and pH; I use
ultra pure water, I've cleaned glasswear 'till it squeaks...no
change....it's still yellow.

Sections seem to stain okay, although the stain goes bad very
quickly, sometimes even on the grid itself. Since this can be a
problem with colorless lead citrate, I'm not sure the yellowing is
the cause. I'd sure appreciate any feedback!

Thanks,
Margaret
--
Margaret Dienelt
Plant Pathologist

Electron Microscopy Lab
FNPRU, National Arboretum
ARS, USDA

B. 010A R. 238 BARC-W
10300 Baltimore Avenue
Beltsville, MD. 20705

Telephone: (301) 504-6097
Fax: (301) 504-5096



From daemon Mon Aug 7 08:04:32 2000



From: Windland, Mark J (MN14) :      Mark.Windland-at-honeywell.com
Date: Mon, 7 Aug 2000 08:03:53 -0500
Subject: RE: IC Structure

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Gary,

I agree with Darrell 100%. He has identified all the layers as I see them.
The aluminum always show some grain boundaries after staining the section
with a BOE as mentioned that you have used on these sections. Also, the
aluminum would not be deposited on top of the polysilicon.

Mark

} ----------
} From: "milesd-at-us.ibm.com"-at-sparc5.microscopy.com
} Sent: Friday, August 4, 2000 3:50 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Re: IC Structure
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Gary,
}
} Your suggested process flow is a little different from all I am used to.
} First, what etches has this cross section seen? I would venture a
} guess that the gate is polysilicon, the layer that forms around the poly
} gate is a CVD (chemical vapor [or is that vapour] deposition) oxide.
} The next layer is a softer glass deposited and then re-flowed (or
} spun on). The next layer (marked poly Si), I would guess to be
} aluminum. The next layer (marked Al), I would guess to be CVD
} oxide, and the layer on top of that, (marked PSG) might be oxide or
} silicon nitride.
}
} Mind you, these are all just guesses. In my small world, I have never
} seen aluminum deposited directly on polysilicon. (That does not
} mean that it is never done) Aluminum usually shows some texture in
} a decorated cross section. It is not usually a smooth surface. That
} level has the appearance I am accustomed to seeing on oxide
} layers (both gray tones and surface). Most processes define well
} regions, grow the field oxide, grow the gate oxide, put on the gates,
} implant source/drains (self aligned to the gates), and then build up
} from there. The interlevel dielectrics must be deposited, they cannot
} be grown, like the gate oxide and the field oxide.
}
} I looked through my books, and found a couple with basic process
} descriptions:
}
} Physical Design of CMOS Integrated Circuits
} using L-EDIT
} by John Uymura
} ISBN 0-534-94326-8
} PWS Publishing Co.
} 20 Park Plaza
} Boston, MA 02116-4324
}
} Microelectronic Processing
} An Introduction to the Manufacture of Integrated
} Circuits
} by W. Scot Ruska
} ISBN 0-07-054280-5
} McGraw-Hill Book Company
}
} [No financial connections with these books. I have taken
} several continuing education classes with John, and we
} have become friends from this long association]
}
} Darrell
}



From daemon Mon Aug 7 08:13:53 2000



From: Ken Tiekotter :      tiekotte-at-up.edu
Date: Mon, 7 Aug 2000 08:05:14 -0500
Subject: donation of a Zeiss EM9s2

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Anyone out there interested in obtaining a working 9s2 in whole or in part
should let me know ASAP. It will otherwise go to the recycle dump.
-Ken

----------
Ken Tiekotter, Adjunct Professor
The University of Protland
Dept. of Biology
5000 N. Willamette Blvd.
Portland, OR 97203

Tel. contact number: (503) 413-5391





From daemon Mon Aug 7 09:16:12 2000



From: Melissa Troost :      m-troost-at-northwestern.edu
Date: Mon, 7 Aug 2000 09:04:49 -0500
Subject: Job Opening

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Specimen Preparation Engineer
Northwestern University

The Electron Probe Instrumentation Center (EPIC) at Northwestern University
has an immediate opening for a specimen preparation expert. EPIC is a part
of the world renowned Materials Research Center (MRC) and the Department of
Materials Science & Engineering at Northwestern.
The EPIC facility serves over 120 users in all aspects of Scanning and
Transmission Electron Microscopy. The role of the specimen preparation
engineer is to assist users with their specimen preparation needs, including
instruction in TEM and SEM sample preparation using IBT, FIB, PIPS,
electropolishing, ultramicrotomy, cutting/grinding/polishing, vacuum
evaporation etc.
All microscopes in EPIC are under full service contract. Thus, the duties
include training students/users, development of specialized techniques and
applications, minor maintenance, record keeping and billing.
A BS or technical degree in physical/biological sciences is required. The
candidate must have hands-on experience in all aspects of specimen
preparation as well as considerable familiarity with digital acquisition,
processing and computer assisted techniques. All levels of experience will
be considered. Compensation will be commensurate with experience and
qualifications.
Send cover letter, resume and three references to:
Prof. Vinayak P. Dravid, Director EPIC
Materials Science & Engineering
Northwestern University, 3013A MLSB
Evanston, IL 60208
E-mail: v-dravid-at-northwestern.edu
Fax: (847) 491-7820
http://epic.ms.nwu.edu/epic/index.htm

Northwestern University is an Affirmative Action/Equal Opportunity Employer.
Hiring is contingent upon eligibility to work in the United States.





From daemon Mon Aug 7 09:52:06 2000



From: Debby Sherman :      sherman-at-btny.purdue.edu
Date: 07 Aug 2000 10:06:51 -0500
Subject: computer log-in system

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I thank Jan for supplying the following information.


-----Original Message-----
} From: Ringnalda, Jan [mailto:JRingnalda-at-FEICO.com]
Sent: Monday, August 07, 2000 9:12 AM
To: 'Ingber, Bruce F.'; Long, Jo; Piscopo, Irene
Cc: Schaub, Daniel; Ringnalda, Jan; Rice, Trisha (CS); Booth, Steve;
'sherman-at-btny.purdue.edu'

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Folks,
I requested information on a computer log-in system a week ago and received a number of replys. I am pursuing some of them including the program written by Tom Ickes which is not available at the moment but may become available soon. Also, I did install the freeware: CM Lock from FEI and it does work great as a means to control access to the TEM. However, it does not record users and use time for later billing. I am still investigating how we can record the log-ins for this purpose.
What I did receive is below:

------------------------
FEI Co. (www.feico.com) has on their web site number of useful freeware
software for Philips CM microscopes. One of them is "CM lock". The program
runs on Win box and does what you asked for. Give them a try. I use is on my
CM300 to define different levels of privileges for my users and disable
microscope controls for off time.

Jerzy Gazda, Ph.D.

---------------

We have a rather good system here that does what you want and quite a lot
more. It will handle several machines. It keeps track of account numbers and
summaries billing information. The system was developed by Tom Ickes who was
an employee here at the time. He has since left Lehigh but may be interested
in setting up a system for you (for a fee, I imagine). The system is well
designed in that it uses off the shelf parts, is controlled by a PC and has
software that has been very well constructed.

His e-mail is:
tomickes-at-cswebmail.com

Good luck,
Alwyn Eades

---------------------

Debby,

I might suggest that you add a digital I/O card that will supply analog
outputs from the computer used to run the TEM. These outputs could then be
used for a type of interlock for turning on the HT. I would not suggest
switching the HT itself. Once this was in place, with software like Visual
Basic or National Instruments LabWindows or LabView you can write a routine
to control the HT, mark time, and write user info to a spread sheet. I might
suggest you contact National Instruments Http://www.natinst.com as they can
supply all you need for this project. On another thought, if your
controlling computer is a PC and can run Windows NT the password issue is
much easier since it has controlled log on features built in. Not a specific
answer but hope this is useful to you.

Roy Beavers

--------------

Dear Debby,

I have recently contacted Richard Benassi at BEI in Maryland. He makes the
exact control mechanisms/software you are talking about. I think he's a bit
pricey ($1,000 for 2 control boxes w/software), but that's just my opinion...

I too am looking to control & monitor microscopes, so if you come across
something else would you be so kind as to pass the information on to me?

Thanks and good luck,

Jim

--------

If I come up with additional information, I'll send it on.
Debby

Debby Sherman, Manager Phone: 765-494-6666
Microscopy Center in Agriculture FAX: 765-494-5896
Dept. of Botany & Plant Pathology E-mail: sherman-at-btny.purdue.edu
Purdue University
1057 Whistler Building
West Lafayette, IN 47907-1057




From daemon Mon Aug 7 11:48:45 2000



From: Melissa Troost :      m-troost-at-northwestern.edu
Date: Mon, 7 Aug 2000 11:36:06 -0500
Subject: job opening

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html
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id xma000203; Mon, 7 Aug 00 11:36:49 -0500
Message-ID: {007701c0008d$95991440$e6256981-at-ms.northwestern.edu}



Specimen Preparation Engineer
Northwestern University

The Electron Probe Instrumentation Center (EPIC) at Northwestern University
has an immediate opening for a specimen preparation expert. EPIC is a part
of the world renowned Materials Research Center (MRC) and the Department of
Materials Science & Engineering at Northwestern.
The EPIC facility serves over 120 users in all aspects of Scanning and
Transmission Electron Microscopy. The role of the specimen preparation
engineer is to assist users with their specimen preparation needs, including
instruction in TEM and SEM sample preparation using IBT, FIB, PIPS,
electropolishing, ultramicrotomy, cutting/grinding/polishing, vacuum
evaporation etc.
All microscopes in EPIC are under full service contract. Thus, the duties
include training students/users, development of specialized techniques and
applications, minor maintenance, record keeping and billing.
A BS or technical degree in physical/biological sciences is required. The
candidate must have hands-on experience in all aspects of specimen
preparation as well as considerable familiarity with digital acquisition,
processing and computer assisted techniques. All levels of experience will
be considered. Compensation will be commensurate with experience and
qualifications.
Send cover letter, resume and three references to:
Prof. Vinayak P. Dravid, Director EPIC
Materials Science & Engineering
Northwestern University, 3013A MLSB
Evanston, IL 60208
E-mail: v-dravid-at-northwestern.edu
Fax: (847) 491-7820
http://epic.ms.nwu.edu/epic/index.htm

Northwestern University is an Affirmative Action/Equal Opportunity Employer.
Hiring is contingent upon eligibility to work in the United States.





From daemon Mon Aug 7 11:51:59 2000



From: Olga Shaffer :      ols0-at-lehigh.edu
Date: Mon, 07 Aug 2000 12:42:28 -0400
Subject: web page on latex microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Folks:

I have begun a web page titled Microscopy of Latex and Latex Films. It's
not quite done, still working on it but have a look.

The address is www.lehigh.edu/~ols0/microscopy.html.


Olga Shaffer
Emulsion Polymers Institute
Lehigh University
Bethlehem, PA




From daemon Mon Aug 7 14:03:18 2000



From: Geoff Williams :      willi1gl-at-cmich.edu
Date: Mon, 07 Aug 2000 14:52:45 -0400
Subject: RMC Contact?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I was just informed by our Purchasing department that their RMC contact
#s no longer work.

Is there anyone out there that can point me to the direction of the
company that might or might not still be RMC that can provide parts for
our MT2B?

Please excuse me if this answer is common knowledge, I just can't seem
to find the information.

TIA,
Geoff Williams

Electron Microscope Facility Supervisor
Biology Department
Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859

Geoffrey.Lloyd.Williams-at-cmich.edu
517 774-3576
517 774-3462 (fax)





From daemon Mon Aug 7 14:31:38 2000



From: RCHIOVETTI-at-aol.com
Date: Mon, 7 Aug 2000 15:22:44 EDT
Subject: Re: RMC Contact?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In a message dated 08/07/2000 12:10:58 PM US Mountain Standard Time,
willi1gl-at-cmich.edu writes:

{ { I was just informed by our Purchasing department that their RMC contact
#s no longer work.

Is there anyone out there that can point me to the direction of the
company that might or might not still be RMC that can provide parts for
our MT2B?

Please excuse me if this answer is common knowledge, I just can't seem
to find the information.

TIA,
Geoff Williams
} }

Geoff,

RMC was acquired by Ventana Medical Systems. You can reach them by calling
1-800-227-2155, Fax no. is 520-887-2558.

I believe that Greg Becker, the former RMC EM Product Manager, is now with
Ventana.

Good luck!

Bob Chiovetti



From daemon Mon Aug 7 15:02:07 2000



From: Geoff Williams :      willi1gl-at-cmich.edu
Date: Mon, 07 Aug 2000 15:52:54 -0400
Subject: RMC Contact - Thanks - Got it

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


thank you to the folks that provided contact info so quickly


Geoff Williams

Electron Microscope Facility Supervisor
Biology Department
Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859

Geoffrey.Lloyd.Williams-at-cmich.edu
517 774-3576
517 774-3462 (fax)





From daemon Mon Aug 7 22:38:54 2000



From: Steve Buckingham :      sbucks-at-charter.net
Date: Mon, 7 Aug 2000 23:13:38 -0400
Subject: pumping oxygen

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All,
I was wondering if anyone had experience or comments on how much
oxygen can safely be pumped before one has to opt for fomblin type oil in
rotary pumps. Does the same limit apply to turbo pump oil, and do the pumps
need to be completely cleaned for the changeover? I believe I've heard that
it is unwise to try to clean a pump and reuse it, with the change of oil?
Also, are there any less expensive sources for oils that can be safely used
when pumping oxygen.
Any comments, suggestions, or hints would be welcome.

Many Thanks,

Steve Buckingham
Excellatron Solid State
(770) 438 2201
All the Best,

Steve.
Kratos Analytical Inc.
(770) 251 6490
"Fill your bowl to the brim, and it will spill.
Keep sharpening your knife, and it will blunt.
Chase after money and security, and your heart will never unclench.
Care about people's approval, and you will be their prisoner.
Do your work, then step back... The only path to serenity."
Lao-tze. Tao te Ching






From daemon Mon Aug 7 22:59:28 2000



From: Jean Howard :      jmhowrd-at-hotmail.com
Date: Tue, 08 Aug 2000 03:45:52 GMT
Subject: TEM- Soil samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Listers:

Does anyone use TEM electron diffraction patterns for identifying silica in
soil samples? I know that X-ray diffraction is a suitable technique, but I
have been asked to find an alternative method such as TEM. If this is
possible what would the sample prep entail?

Thanks in advance

Jean Howard

________________________________________________________________________
Get Your Private, Free E-mail from MSN Hotmail at http://www.hotmail.com




From daemon Tue Aug 8 04:36:27 2000



From: Ken Tiekotter :      tiekotte-at-up.edu
Date: Tue, 8 Aug 2000 02:22:39 -0700 (PDT)
Subject: Re: RMC Contact?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


RMC was purchased by Ventana Medical Systems, 1-800-356-3452 or
1-800-277-4613ext3023. Greg Carter is the ultramicrotome service
manager. I had him visit my lab to service our MT2B in December,
1999. Let me know if this does not work.

Sincerely,
Ken

On Mon, 7 Aug 2000, Geoff Williams wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I was just informed by our Purchasing department that their RMC contact
} #s no longer work.
}
} Is there anyone out there that can point me to the direction of the
} company that might or might not still be RMC that can provide parts for
} our MT2B?
}
} Please excuse me if this answer is common knowledge, I just can't seem
} to find the information.
}
} TIA,
} Geoff Williams
}
} Electron Microscope Facility Supervisor
} Biology Department
} Brooks Hall
} Central Michigan University
} Mt. Pleasant, MI 48859
}
} Geoffrey.Lloyd.Williams-at-cmich.edu
} 517 774-3576
} 517 774-3462 (fax)
}
}
}
}




From daemon Tue Aug 8 05:47:40 2000



From: Dick Briggs :      rbriggs-at-Science.Smith.edu
Date: Tue, 8 Aug 2000 06:35:15 -0400
Subject: Re: RMC Contact?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Geoff,

You might also try getting your MT2B (I thought I was the only one
still using one!) from Bill McGee at Microtome Services Company -
315-451-1404. He is a great guy to deal with, very reasonably
priced, and has provided me with excellent service for a long time.

Hope this helps.

Dick Briggs
Biology Department
Smith College



From daemon Tue Aug 8 06:50:37 2000



From: Phillip Rutledge :      prutledge-at-ars.usda.gov
Date: Tue, 08 Aug 2000 05:33:55 -0600
Subject: Re: TEM: yellow PbCitrate

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} } } Margaret Brannigan {brannign-at-asrr.arsusda.gov} 08/07/00 14:59 PM } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Hi,

Does anyone know why freshly made lead citrate sometimes comes out
yellow, and what adverse effects, if any, result? In going over
past threads on the subject, it seems most people have a relatively
colorless product as the end result, but a few report the yellow
color as normal. After years of making up "colorless" lead citrate,
mine is now yellow. I've toyed with NaOH sources and pH; I use
ultra pure water, I've cleaned glasswear 'till it squeaks...no
change....it's still yellow.

Sections seem to stain okay, although the stain goes bad very
quickly, sometimes even on the grid itself. Since this can be a
problem with colorless lead citrate, I'm not sure the yellowing is
the cause. I'd sure appreciate any feedback!

Thanks,
Margaret
--
Margaret Dienelt
Plant Pathologist

Electron Microscopy Lab
FNPRU, National Arboretum
ARS, USDA

B. 010A R. 238 BARC-W
10300 Baltimore Avenue
Beltsville, MD. 20705

Telephone: (301) 504-6097
Fax: (301) 504-5096


Margaret,

Lead citrate turning yellow is a new one on me. I've never heard of that peoblem. When I make lead citrate, I use a 100ml bottle that previously was used for HBSS ( I find they work better than a volumetric flask, easier to use), put the bottle on a magnetic sirrer, add lead citrate, close the bottle, let it stir for a while then had concentrated NaOH ( Fisher catalog # ss277) let it stir until solution clears, put it in refrigerator overnight (keeping it stored in refrig. at all times until use). Never turned yellow on me. I've made up my stain this way for almost 30 years and it never turned yellow and I've been able to use the stain for several months. If you have any questions, call me.

Phil Rutledge
USDA/ARS
voice: 410 788-4136 or 2120
e-mail:prutledge-at-ars.usda.gov
fax: 410 788-4399





From daemon Tue Aug 8 07:32:57 2000



From: khalid-at-eucmax.sim.ucm.es
Date: Tue, 08 Aug 2000 14:27:47 +0200
Subject: unscribe

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From daemon Tue Aug 8 08:25:44 2000



From: Ping Liu :      ping.liu-at-sandvik.com
Date: Tue, 8 Aug 2000 14:50:00 +0200
Subject: Electron diffraction of silica in soil samples

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Yes, Although we do no do with soil sample we do identify particles
from different kinds of oxides on the metal surface. With support of
holly carbon film electron diffraction can be obtained from silica if
the size of silica particles is around, say 100 nm or less. We have
obtained electron pattern from extracted silica.

yours Sincerelly

Ping Liu




Listers:

Does anyone use TEM electron diffraction patterns for identifying silica in
soil samples? I know that X-ray diffraction is a suitable technique, but I
have been asked to find an alternative method such as TEM. If this is
possible what would the sample prep entail?

Thanks in advance

Jean Howard





From daemon Tue Aug 8 08:26:54 2000



From: Anthony Greco :      tgreco-at-seas.marine.usf.edu
Date: Tue, 08 Aug 2000 09:11:40 -0400
Subject: TEM/Wrinkled Spurr Sections

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I have a constant problem of regularly spaced wrinkles on my Spurr
sections on the TEM. I stain with uranyl acetate in 50% ethanol for
about 30 minutes followed by stable lead for 15 minutes.(Wrinkling is
even worse with methanolic UA). I am certain the problem has something
to do with staining since few wrinkles appear when I observe unstained
sections. I also rarely encounter wrinkled sections with Embed 812
sections when using aqueous UA stains. Any suggestions?

Tony Greco




From daemon Tue Aug 8 09:51:02 2000



From: William Tivol :      tivol-at-wadsworth.org
Date: Tue, 08 Aug 2000 10:28:03 -0400
Subject: Re: TEM- Soil samples

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Jean Howard wrote:

} Does anyone use TEM electron diffraction patterns for identifying silica in
} soil samples? I know that X-ray diffraction is a suitable technique, but I
} have been asked to find an alternative method such as TEM. If this is
} possible what would the sample prep entail?
}

Dear Jean,
I have taken selected-area ED (SAED) patterns of minerals in soil
samples, but I have not yet attempted to get a structure. Of course, identi-
fying a silicate of known structure from one or more zone-axis patterns
is a simpler problem. The sample preparation is very easy--I just suspend
the soil in water, dilute to the appropriate degree so that the soil particles

are well dispersed, put a few microliters on a carbon-formvar grid, and
air-dry. When you find a particle of interest, you may have to orient the
grid to get a zone axis, so you need a double-tilt or tilt-rotation stage.
Once you have the proper orientation, you can ascertain the crystal form
and measure the unit cell parameters and intensities. If you have an image
simulation program, you can calculate the predicted intensities from a
trial structure (which matches the unit cell parameters) and see what kind
of agreement you get. This should work even if there is some dynamical
scattering. You could try an ab initio structure determination if the cry-
stal is thin enough and the voltage high enough so that dynamical scat-
tering is not significant, but this is much more difficult and requires
much more data. See Doug Dorset's book, Structural Electron Crystal-
lography for a good overview. Good luck.
Yours,
Bill Tivol




From daemon Tue Aug 8 10:06:57 2000



From: Olga Shaffer :      ols0-at-lehigh.edu
Date: Tue, 08 Aug 2000 10:48:31 -0400
Subject: Re: Web Page: "Microscopy of Latex and Latex Films"

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Hi Paul:

Thanks for the positive feed-back and the chance for discussion.

1. If you notice the figure is labeled seed. This seed is a co-polymer of BA-B.
The reason for the difference in shadow length even after cross linking with
OsO4 is the heterogeneous composition. I think this is due to the polymerization
rates of the two monomers. Butadiene polymerizes faster than BA so it will form
particles first and the BA follows. This was then to be the seed in a core-shell
latex. If you look at the third figure on the web page you will see the final
latex.

2. You are right, at just the right orientation the core should look centered,
but the chance of that happening frequently is small. However, if you look at
the group of three particle around 11:00 you will see the middle particle having
a fairly centered core.

If you have any other questions please e-mail me. Discussion is good.

Olga Shaffer

"Gerroir, Paul J" wrote:

} Hi Olga,
} An impressive start to your web page! The polymer chemists/engineers here
} will find these images very exciting.
} I have two comments/questions.
} 1. In your first example of TEM - Staining you have illustrated that a "very
} short shadow indicating very little PB" It is not clear in this example of
} shadowing and staining whether the system, PB/PBA is a copolymer or as I
} suspect the seed referred to is PBA and the shadowing gives an indication of
} the amount of shell, i.e. PB associated with the seed or core.
} 2. In your second example of TEM - Staining you have pointed out the
} off-center nature of the PS core. If we think of these images in two
} dimensions and the latex spheres being randomly oriented why don't we see
} any particles where the core appears to be more centered? If you were to
} view these two-dimensional spheres from the left or right in your images
} wouldn't a picture of a more centralized core result? Make a simple
} three-dimensional model and try this experiment, then perhaps you can offer
} me an explanation. Do you think it a little puzzling that these particles
} all orient similarly?
}
} Regards,
} Paul
}
} Paul J. Gerroir
} Microscopy
} Materials Characterization
} Xerox Research Centre of Canada
} 2660 Speakman Drive
} Mississauga, Ontario L5K 2L1
}
} Phone: (905) 823-7091, ext. 216
} FAX: (905) 822-7022
} e-mail: paul.gerroir-at-crt.xerox.com




From daemon Tue Aug 8 10:28:10 2000



From: jekman-at-uwm.edu
Date: Tue, 8 Aug 2000 10:16:44 -0500
Subject: Re: Electron diffraction of silica in soil samples

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Yes TEM electron diffraction patterns for identifying silica in soil
samples can be done. One quick way is to sonicate some of the
sample in water maybe with some detergent added (do different
dilutions because what looks like a little bit can be a lot in the
TEM.) Then take an aliquot .5-1ul and drop it onto a carbon
film/holey grid. (we used to collapse our own MCE filters after
lightly coating them with carbon) Allow the grid to dry, then place in
scope. We used to start with the most dilute prep first and would
get results off it.

good luck




} Listers:

} Does anyone use TEM electron diffraction patterns for identifying
} silica in soil samples? I know that X-ray diffraction is a suitable
} technique, but I have been asked to find an alternative method
} such as TEM. If this is possible what would the sample prep
} entail?

} Thanks in advance

} Jean Howard
Jon Ekman
Associate Research Specialist
University of Wisconsin Milwaukee
414-229-6471




From daemon Tue Aug 8 11:01:16 2000



From: Dr Eric Lachowski :      che136-at-abdn.ac.uk
Date: Tue, 8 Aug 2000 16:44:55 +0100 (BST)
Subject: electron diffraction of silica in soils

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Dear Jean,
I've done ED studies of soils from time to time. I
have not done the sample preparation myself, but it's not
too difficult. You need to disperse the soil in a liquid
such as water or 2-propanol and let a drop of the
suspension dry on a normal TEM grid. An ultrasonic bath is
ideal for this. If the silica grains are substantially
bigger than the clays, there may be fractionation,
resulting in a reduced yield of silica as it can fall to
the bottom of the suspension. Care is therefore needed in
sampling. If the silica grains are big enough (one micron
or more) you can use a double-tilt or rotation-tilt holder
to align the crystallites to get a sensible zone pattern
which will make interpretation easier. If the grains are
v. small and you need to use a micro-diffraction technique
you may find the tilting process drives you crazy as it's
v. difficult to keep the grain in the beam during tilting.

Thr camera length of the microscope varies with specimen
height, and specimen height varies with tilting in
duoble-tilt holders. Generally, external calibration (e.g.
camera length vs objective current) is not wholly
satisfactory but in your case may be adequate. The
projector astigmatism needs to be very well corrected if
accurate d-spacings are to be obtained. A sure-fire way
round this is to use an internal standard such as a poly-
crystalline Al or Au film evaporated onto the carbon
support film. This produces a ring pattern
superimposed on the spot pattern, allowing the
actual camera length in any direction to be measured
directly. (Modern sputter coaters produce Au films where
the grain size is too small to give sharp rings, so it has
to be evaporation from a hot W wire.)

Not all silica in soils is crystalline, though, and my
preferred method is to do TEM-EDX, which can resolve very
small areas as the limiting factor is the minimum probe
size that still gives a reasonable number of counts. It's
also a lot quicker and easier.

Regards,
Eric

----------------------
Dr Eric Lachowski
Department of Chemistry
University of Aberdeen
Meston Walk
Old Aberdeen AB24 3UE
Scotland
+44 (0)1224 272934 fax 272921
e.lachowski-at-abdn.ac.uk






From daemon Tue Aug 8 12:24:14 2000



From: Dohnalkova, Alice :      Alice.Dohnalkova-at-pnl.gov
Date: Tue, 08 Aug 2000 10:06:15 -0700
Subject: TEM- Soil samples

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A sophisticated method of soil sample analysis by high resolution TEM is thin
sectioning. That requires complete sample dehydration in 100 % EtOH, gradual
infiltration with LR White, (representative) suspension sampling followed by
embedding in gel capsules, curing, and thin sectioning on a microtome. I
recommend to collect sections on Cu / carbon lacey grids. No need to osmicate or
post-stain unless you're interested in soil organisms.

Alice Dohnalkova
Environmental Microbiology
Battelle, PNNL
MS P7-50
Richland, WA 99352
tel. (509) 372-0692
fax (509) 376-1321


-----Original Message-----
} From: Jean Howard [SMTP:jmhowrd-at-hotmail.com]
Sent: Monday, August 07, 2000 8:46 PM
To: Microscopy-at-sparc5.microscopy.com


Listers:

Does anyone use TEM electron diffraction patterns for identifying silica in
soil samples? I know that X-ray diffraction is a suitable technique, but I
have been asked to find an alternative method such as TEM. If this is
possible what would the sample prep entail?

Thanks in advance

Jean Howard

________________________________________________________________________
Get Your Private, Free E-mail from MSN Hotmail at http://www.hotmail.com




From daemon Tue Aug 8 13:01:17 2000



From: Andrew Ochalski :      aochalsk-at-science.uottawa.ca
Date: Tue, 8 Aug 2000 14:51:19 -0500
Subject: Anti-mitochondrial and anti-vimentin antibodies?

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Excuse me folks, but isnt everyone putting the carriage before the horse?
Dont you think that as scientists you need to be a bit less eager and a bit
more conscientious to tell Jean Howard yes when there are more important
questions to be answered?

Jean; why do you have to find an alternative method? Do you not have a
diffractometer and a competent XRD person on staff? If not, why bother
getting into a can of worms? Are you to analyze the soils for the regulatory
crystalline silica content of 0.1% by weight for hazmat, product labelling?
Why analyze it by a method that is not accepted? XRD is the accepted
method for analyzing air samples for the respirable fraction by NIOSH.
Almost all competent scientists that I know of analyze bulk materials with
several modifications depending on the sample.

If you go and do it by TEM, you could be putting yourself in a dangerous
liability situation causing question of your capabilities as a scientist.
Doing it by TEM is frought with problems, and how do you propose to
accurately and precisely quantify it at the 0.1% by weight level?.......you
cant do it, at least not reproducibly.

The XRD method is the only way to do it if you want as close to real numbers
as you can get. There are plenty of labs that will analyze it wrong for $50
a pop by XRD. I know.....I have been doing good analyses for 10 yrs, and it
takes a hell of a lot more than $50 worth of work. You need to find out what
the sample is composed of first, then remove components gravimetrically,
with acid dissolutions or thermal treatments to render phyllosilicates
amorphous. Most micas and clays have interfering peaks at the primary,
secondary and tertiary quartz peaks. How do you know you arent analyzing
beta or quasi metastable polymorphs of tridymite and cristobalite? You dont.

I can recommend some top notch people to verify what I have said, and who
can do it by XRD better than most. Let me know. I am disappointed at how
quickly so many people are responding to say it can be done by TEM without
really thinking about the problems that need to be addressed first. Good way
to chop your reputation up.

Lou Solebello


----- Original Message -----
} From: Ping Liu {ping.liu-at-sandvik.com}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Tuesday, August 08, 2000 5:50 AM




Fellow Microscopists,

I am looking for 2 reliable, "clean" polyclonal (preferred) or monoclonal antibodies for use with
aldehyde/detergent-treated or ethanol-treated cultured cells (HeLa). One antibody would be against
vimentin, and the other against a protein that allows clear visualization of mitochondria. Any
suggestions? Thank you in advance.
Regards,

Andrew Ochalski,
Microscopy Technician,
Dept. of Biology,
University of Ottawa
Room 108, Gendron Bldg.
130 Louis Pasteur,
Ottawa, Ontario
CANADA
K1N 6N5
613-562-5800 x6343
FAX: 613-562 5486



From daemon Tue Aug 8 14:45:33 2000



From: Tamara Howard :      howard-at-cshl.org
Date: Tue, 8 Aug 2000 15:29:40 -0400 (EDT)
Subject: Re: TEM/Wrinkled Spurr Sections

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Have you tried aqueous stains for the Spurr's? I've used aq. UA/Pb and had
nice staining. If you have some stain-resistant plant material, you can
use KMnO4 instead of UA - it stains well.

Tamara Howard
CSHL


On Tue, 8 Aug 2000, Anthony Greco wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I have a constant problem of regularly spaced wrinkles on my Spurr
} sections on the TEM. I stain with uranyl acetate in 50% ethanol for
} about 30 minutes followed by stable lead for 15 minutes.(Wrinkling is
} even worse with methanolic UA). I am certain the problem has something
} to do with staining since few wrinkles appear when I observe unstained
} sections. I also rarely encounter wrinkled sections with Embed 812
} sections when using aqueous UA stains. Any suggestions?
}
} Tony Greco
}
}
}




From daemon Tue Aug 8 17:27:36 2000



From: Ypaulwang-at-aol.com
Date: Tue, 8 Aug 2000 18:14:45 EDT
Subject: Re: unscribe

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To Subscribe/Unsubscribe:
} From: ypaulwang-at-aol.com

unscribe please

Thank you.

Paul Wang



From daemon Tue Aug 8 17:34:48 2000



From: Dunlap, Jonathan C. :      Jonathan.Dunlap-at-sylvania.com
Date: Tue, 8 Aug 2000 18:25:19 -0400
Subject: Amray 1800 50 kV Power Supply

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Hello all,
Does anybody have any experience in getting 50 kV Power Supplies for
Amray 1800 series electron microscopes fixed. The power supply was
manufactured by CPS, a company now out of Oregon. Approximately two weeks
ago we fedex'd them our supply for repair (which cost an arm and a leg), and
after all this time, they're still can't even give me an approximate date
when it will be completed. They haven't even diagnosed it yet. Obviously
we're dead in the water with out it, and work is piling up. Does anybody
know of any other options. Possibly other shops that can repair these
supplies, or possibly another source for a different supply. I'm so
frustrated at the fact that they didn't look at it for a week, and the fact
that they have no clue when it will be done. I of all people can understand
being busy, but I can't live another two weeks with the e-scope down for the
count. If anybody has any ideas that would help to restore my sanity, or
get my supply fixed, I would be forever indebted.


Thanks in advance,
~Jonathan Dunlap




Jonathan Dunlap
Analytical Laboratory Manager
Osram Sylvania Inc.
816 Lexington Avenue
Warren, PA 16365
Ph: 814-726-6991
Fax: 814-726-6956






From daemon Tue Aug 8 18:35:41 2000



From: MELISSA ANN LEWIS :      lewisma-at-medicine.ufl.edu
Date: Tue, 8 Aug 2000 18:23:00 -0500
Subject: vibratome

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I am looking for a used vibratome to purchase. If anyone knows of one
please contact me. No special needs just a regular vibratome. Thanks
Melissa





From daemon Tue Aug 8 20:18:26 2000



From: Dave Phelan :      emudp-at-mail.newcastle.edu.au
Date: Wed, 09 Aug 2000 11:07:31 +1000
Subject: New heater element needed for aging Critical Point Dryer

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G'day

I have an aging (~15years old) Balzers Critical Point Dryer that has
a burnt out heater. The dryer is type 11120 B no. 337. The heater
is a plastic strip 20 cm long and 3.5cm wide which seems to have
carbon or graphite laminated into the strip and connecting wires
are soldered onto each end. This wraps around the chamber of the
unit and was held there by heat shrink wrap. Does anyone know if
you can get replacements for this heater ( in Australia would
help!)? I am waiting to hear from Balzers but need to get it fixed
quickly.

Regards
Dave



Dave Phelan
EM/X-Ray Unit
University of Newcastle
NSW 2308
AUSTRALIA
Ph 02 4921 5667
Fax 02 4921 7019
emudp-at-mail.newcastle.edu.au




From daemon Tue Aug 8 21:49:18 2000



From: Gary Gaugler :      gary-at-gaugler.com
Date: Tue, 08 Aug 2000 19:30:10 -0700
Subject: Re: Amray 1800 50 kV Power Supply

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I have worked on their 30KV units in my Amray scopes. The
problem is either the driver transistor or the HV generator
block.

I have the schematics for the 30KV CPS unit. The HV block was
about $250. The transistor was $7 from Radio Shack. I
zapped the unit when the stub made hard contact with the
pole piece in my 1600T. The CPS units in my 1830 and 1910
have never failed.

I found CPS to be quite helpful. I guess that this may have changed.

gary g.


At 03:25 PM 8/8/00, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From daemon Tue Aug 8 22:35:53 2000



From: Jean Howard :      jmhowrd-at-hotmail.com
Date: Wed, 09 Aug 2000 03:24:11 GMT
Subject: TEM Soil samples

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Dear Lou Soebello and other Listers:

First of all, let me clarify what is being requested. The lab is interested
in the regulatory crystalline silica content in soils. Currently the
samples are being contracted out for XRD because there is no diffractomer or
XRD scientist in house. Their results are reliable and NIOSH acceptable.

The question was whether the TEM (which is in lab) could be used for this
analysis instead of purchasing a diffractometer. Let me say that I have no
experience with minerals by TEM. My work has been primarily with polished
metal samples. I had some doubts about using TEM for this type of work
since I was not successful in finding literature on such.

I appreciate all the responses and apologize for not being more specific.
No one's competency is in question here. I have received numerous
suggestions but it is clear to me that the most reliable method is XRD.
Should I need to speak to someone further I will not hesitate to contact you
Lou.

Thank you all

Jean Howard
________________________________________________________________________
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From daemon Wed Aug 9 03:27:30 2000



From: Baggethun, Paul :      Paul.Baggethun-at-alcoa.com
Date: Wed, 9 Aug 2000 04:11:37 -0400
Subject: RE: Grain size.......

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Provided you can obtain a binary or thresholded grain boundary image, there
are a number of
general image analysis programs available - free of charge - that will
automatically extract the grain size. Try:
* "ImageJ" http://rsb.info.nih.gov/ij/index.html
* "Scion Image" http://www.scioncorp.com/frames/fr_scion_products.htm
* "Image Tool" http://macorb.uthscsa.edu/dig/itdesc.html

Cheers,
Paul Baggethun


} ----------
} From: Maria Luiza[SMTP:luiza-at-ppgem.faenquil.br]
} Sent: Monday, July 31, 2000 7:31 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Grain size.......
}
} Hi. I am a studant from Brazil and I´m trying to measure the grain size of
} my samples (They are Niobium 9%Tantalun).
} I know there are a lot of softwares available on the internet, but I don´t
} know where and if there is a specific program to analise the grain size of
} metals.
} Please, remember that I work at Brazil, so I don´t have any money buy a
} software. We allways try to do the best we can.
}
} Thank you very much..... João Paulo Barros Machado.....
}
} Please answer this email for: jpbm-at-easygold.com.br
}
}
}



From daemon Wed Aug 9 07:29:47 2000



From: Gary Coulton :      g.coulton-at-ic.ac.uk
Date: Wed, 09 Aug 2000 13:14:03 +0100
Subject: ICHC 2000

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Hi Everyone,

If you are making a last minute decision as to which conferences to attend
this summer/fall then take a look at ICHC 2000 "Cell Biology and Imaging
Tools for the New Century". 3-8th Sept. 2000 York, UK

Take look at the fantastic Final Programme at
http://www.med.ic.ac.uk/external/ichc_2000

Over 100 leading speakers in 35 symposia

York is a most beautiful medieval walled city, a great place for a
vacation. You can register for the week or if close to York then come for
the day!

Gary Coulton
Organiser ICHC 2000



From daemon Wed Aug 9 09:48:39 2000



From: Shea Miller :      millers-at-em.agr.ca
Date: Wed, 09 Aug 2000 10:25:49 -0400
Subject: enzyme histochemistry

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Hello everyone;
now that the corn has finally started to do something in this miserable excuse for a summer we are having, we have a grad student who would like to do some peroxidase histochemistry on a rather daunting number of samples and varieties.
is there any fixation he can use to preserve both his samples and his enzyme activity? he will not be able to look at everything in a reasonable length of time.
thanks, as always, in advance
shea



Dr. S.Shea Miller
Agriculture & AgriFood Canada
Eastern Cereal and Oilseed Research Centre
Rm. 2068 Neatby Building
Central Experimental Farm
Ottawa, Ontario
Canada K1A 0C6
Phone: (613) 759-1760
Fax: (613) 759-1701
E-mail: millers-at-em.agr.ca




From daemon Wed Aug 9 10:30:20 2000



From: rgriffin-at-eng.uab.edu
Date: Wed, 9 Aug 2000 10:09:05 -0500
Subject: TEM of diamond films

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I have a student that would like to do TEM of diamond films that are
prepared by microwave plasma CVD. The thickness of the films can be
adjusted to make it electron beam transparent. The student believes that he
can flake off pieces of the film.

I'm thinking maybe we could just lay the diamond film on a grid and pop it
into the TEM. Is this a crazy idea? Does anyone have any suggestions?

Thanks,

Robin Griffin



From daemon Wed Aug 9 10:49:37 2000



From: William Tivol :      tivol-at-wadsworth.org
Date: Wed, 09 Aug 2000 11:23:56 -0400
Subject: Re: Electron diffraction of silica in soil samples

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Lou Solebello wrote:

Dear Lou,


} Excuse me folks, but isnt everyone putting the carriage before the horse?
} Dont you think that as scientists you need to be a bit less eager and a bit
} more conscientious to tell Jean Howard yes when there are more important
} questions to be answered?
}

I guess that those of us who answered thought of the question from
an academic/technical viewpoint--true, at least, in my case. Yes, if there
are legal questions about the methods, that is a consideration I did not think
about.


}
} Jean; why do you have to find an alternative method? Do you not have a
} diffractometer and a competent XRD person on staff? If not, why bother
} getting into a can of worms?

To characterize tens to hundreds of individual soil particles that
are
in the micrometer size range can be done in a few (long) sessions on the TEM.
This includes EDS, ED, and imaging. I don't think that the same info can be
obtained in as short a time by XRD.


} Are you to analyze the soils for the regulatory
} crystalline silica content of 0.1% by weight for hazmat, product labelling?

I hadn't considered this aspect.

}
} Why analyze it by a method that is not accepted? XRD is the accepted
} method for analyzing air samples for the respirable fraction by NIOSH.
} Almost all competent scientists that I know of analyze bulk materials with
} several modifications depending on the sample.

However, to analyse many individual particles and distinguish
between, e.g., sodium aluminum silicate and potassium aluminum silicate
is not best done by XRD.

}
}
} If you go and do it by TEM, you could be putting yourself in a dangerous
} liability situation causing question of your capabilities as a scientist.

As a scientist, or as a lawyer?


}
} Doing it by TEM is frought with problems, and how do you propose to
} accurately and precisely quantify it at the 0.1% by weight level?.......you
} cant do it, at least not reproducibly.

To get the distribution of various silicates to a precision of 0.1%

would, indeed, require analysis of many thousand (million?) particles. Of
course, one could, nonetheless, get such precision given enough time.

}
}
} The XRD method is the only way to do it if you want as close to real numbers
} as you can get. There are plenty of labs that will analyze it wrong for $50
} a pop by XRD. I know.....I have been doing good analyses for 10 yrs, and it
} takes a hell of a lot more than $50 worth of work. You need to find out what
} the sample is composed of first, then remove components gravimetrically,
} with acid dissolutions or thermal treatments to render phyllosilicates
} amorphous. Most micas and clays have interfering peaks at the primary,
} secondary and tertiary quartz peaks. How do you know you arent analyzing
} beta or quasi metastable polymorphs of tridymite and cristobalite? You dont.

Here is where the simplicity of the TEM preparation, ability to
take
images, and elemental analyses of individual particles can answer these
questions more quickly than just using XRD. If there is a mixture of two
isomorphous silicates--say one with sodium and one with potassium but
otherwise the same--I would not be optimistic about quantitating them to
the 0.1% level from the ring pattern intensities, but EDS should do the job
easily in a few minutes.

}
} I can recommend some top notch people to verify what I have said, and who
} can do it by XRD better than most. Let me know. I am disappointed at how
} quickly so many people are responding to say it can be done by TEM without
} really thinking about the problems that need to be addressed first. Good way
} to chop your reputation up.

I look to this list as a forum for the discussion of technical
points. As
you said, there are often other considerations. I am happy to give technical
advice where I am knowledgable and leave the decision of whether the other
criteria will be met to someone else.
Yours,
Bill Tivol




From daemon Wed Aug 9 11:17:39 2000



From: Wright, John D. :      jwright-at-dugway-emh3.army.mil
Date: Wed, 9 Aug 2000 11:18:26 -0600
Subject: Maleate buffer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Peroxidase is fairly resistant to glutaraldehyde or Karnovsky's
fixatives, so good morphology should be possible. see JL Hall and
R Sexton (1972) Cytochemical localization of peroxidase activity in
root cells. Planta 108, 103-120.

Date sent: Wed, 09 Aug 2000 10:25:49 -0400
} From: "Shea Miller" {millers-at-em.agr.ca}
To: microscopy-at-sparc5.microscopy.com


Off-and-on since the 1970s I've been preparing uranyl acetate for en bloc
staining using maleate buffer at pH 5.2 adjusted with NaOH. However, the
pKa I for maleic acid is 2.0 and the general rule for buffer selection is
that the the desired pH should be within +/- one pH unit of the pKa value
(p. 46 in Buffer Solutions,The Basics by Beynon and Easterby; IRL Press,
1996). Therefore, maleate buffer appears not to have a good buffering
capacity at pH 5.2. Does anyone understand why maleate was chosen as
bufffer for this procedure, and what I may be missing in my thinking on this
subject? A current reference for this en bloc staining technique is found
in Biomedical Electron Microscopy by Maunsbach and Afzelius, Academic Press,
1999.

/John/

John D. Wright, Ph.D.
West Desert Test Center
Dugway, UT





From daemon Wed Aug 9 12:58:47 2000



From: Al Coritz :      acoritz-at-ventanamed.com
Date: Wed, 9 Aug 2000 10:45:27 -0700
Subject: Re: RMC Contact?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


RMC is still part of Ventana Medical systems Inc. Feel free to contact me
directly for Sales, Applications support & Service issues.

Drop by & see us at M & M


Best,

Al Coritz
North American Sales Manager
Ventana-RMC
Microscopy Products Group
1-800-227-2155 x 2773
Direct: 520-690-2773
Fax: 520-690-2759
Cell: 520-906-3268


-----Original Message-----
} From: Ken Tiekotter [mailto:tiekotte-at-up.edu]
Sent: Tuesday, August 08, 2000 2:23 AM
To: Geoff Williams
Cc: Microscopy Listserver


RMC was purchased by Ventana Medical Systems, 1-800-356-3452 or
1-800-277-4613ext3023. Greg Carter is the ultramicrotome service
manager. I had him visit my lab to service our MT2B in December,
1999. Let me know if this does not work.

Sincerely,
Ken

On Mon, 7 Aug 2000, Geoff Williams wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I was just informed by our Purchasing department that their RMC contact
} #s no longer work.
}
} Is there anyone out there that can point me to the direction of the
} company that might or might not still be RMC that can provide parts for
} our MT2B?
}
} Please excuse me if this answer is common knowledge, I just can't seem
} to find the information.
}
} TIA,
} Geoff Williams
}
} Electron Microscope Facility Supervisor
} Biology Department
} Brooks Hall
} Central Michigan University
} Mt. Pleasant, MI 48859
}
} Geoffrey.Lloyd.Williams-at-cmich.edu
} 517 774-3576
} 517 774-3462 (fax)
}
}
}
}




From daemon Wed Aug 9 14:44:58 2000



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Wed, 9 Aug 2000 09:29:34 -1000 (HST)
Subject: M&M2000 Exhibitor Demonstrations

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


To all of those attending M&M2000:

The MSA Education Committee is once again organizing the FREE Exhibitor
Demonstrations on Tuesday, Aug. 15, 2000 at 5:00 pm. Below is a list of
participants and the titles of their tutorial workshops. If you wish to
attend one of these, please come to the MSA Education booth (now part of
the MSA Mega-Booth) on the Exhibit Hall floor to sign up and receive a
ticket allowing you to attend.

This list may not be exhaustive, so come by the booth to see what has been
added!


Advanced Microscopy Techniques Corp. - Advantage HR Digital Camera
System.

Allied High Tech Products, Inc. - Precision Sample Preparation for
TEM, SEM and PreFIB (Multiple Sample) Thinning Using the Multiprep and
MicroVision Measurement and Archiving Software.

Digital Instruments, Veeco Metrology Group - Surface Metrology
Instrumentation: An Overview of Atomic Force Microscopes, Optical
Interference Microscopes and Stylus Profilers.

E.A. Fischione Instruments, Inc. - Contamination-Free Specimen
Preparation.

EDAX, Inc. - Quantitative Analysis of EDS Data from the Low-Vacuum
SEM.

Electron Microscopy Sciences - Developments in Ultra Small Immunogold
Silver Detection Systems.

Evex Analytical - Basic and Intermediate Features of the Evex
Microanalysis System.

FEI Company - Precautions for Collecting EDS Data in a Low-Vac SEM.

KS Electron Technologies - Up Close with the Worlds Most Affordable
TEM.

LEO Electron Microscopy, Inc. - EFTEM in Practice.

Micro Photonics, Inc. - Non-Destructive 3D Microscopy Using X-Ray
Microtomography.

Microbiology/Syncroscopy - Bring Your World Into Focus with
Syncroscopy.

Motic Incorporation, Ltd. - All-In-One Digital Microscope with
Powerful Software.

NORAN Instruments, Inc. - Phase Identification and Orientation Mapping
by EBSD.

Photon Technology International, Inc. - NovaLight: the NEW Power in
Microscope Illumination.

SouthBay Technology, Inc. - Electropolishing, Tripod Polishing and
Dimpling; Benefits and Limitations.

Ted Pella, Inc. - New Microwave Techniques, Accessories and Equipment.

TSL - #1 Advances in Phase Identification in the SEM.
#2 Orientation Mapping in the TEM.




****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************




From daemon Wed Aug 9 14:45:09 2000



From: rgriffin-at-eng.uab.edu
Date: Wed, 9 Aug 2000 14:25:02 -0500
Subject: TEM of clay in polystyrene

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have a student who would like to do TEM on samples of clay in polystyrene.
The particles are very small (around 1 nm in thickness) and are in
suspension are in suspension. He believes that we could scoop up some
sample onto a carbon film and examine them in an SEM. He has a chemistry
paper where someone did just that. I have a TEM that has a voltage range of
100-200kV. I have never worked on polymers or a clay.

Questions:
Will clay or polystyrene contaminate the TEM? What voltage range is safe?
Any tricks?

By the way I do have a stage that can be liquid nitrogen cooled to minimize
radiation damage.

Thanks for any input.

Robin at UAB



From daemon Wed Aug 9 15:08:15 2000



From: sghoshro-at-nmsu.edu
Date: Wed, 9 Aug 2000 13:54:10 -0600 (MDT)
Subject: Re: enzyme histochemistry

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Shea,

The plant samples can be fixed in 4% paraformaldehyde and 0.5% GTA in
phosphate buffer pH 7.2. I embed in paraffin and make 5-6 micron sections.
I normally use the Vector Lab kit (no financial interest), peroxidase or
alkaline phosphatase to do my enzyme histochemistry and it works
beautifully. You can also use any fluorescent probe to label your protein
of interest.

Good luck,

Soumitra
*************************************************************
Soumitra Ghoshroy Ph.D.
Electron Microscopy Lab
Box 3EML
New Mexico State University
Las Cruces, NM 88003
Tel: 505-646-3600
Fax: 505-646-5665
e-mail:sghoshro-at-nmsu.edu
http://confocal.nmsu.edu/eml

On Wed, 9 Aug 2000, Shea Miller wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hello everyone;
} now that the corn has finally started to do something in this miserable excuse for a summer we are having, we have a grad student who would like to do some peroxidase histochemistry on a rather daunting number of samples and varieties.
} is there any fixation he can use to preserve both his samples and his enzyme activity? he will not be able to look at everything in a reasonable length of time.
} thanks, as always, in advance
} shea
}
}
}
} Dr. S.Shea Miller
} Agriculture & AgriFood Canada
} Eastern Cereal and Oilseed Research Centre
} Rm. 2068 Neatby Building
} Central Experimental Farm
} Ottawa, Ontario
} Canada K1A 0C6
} Phone: (613) 759-1760
} Fax: (613) 759-1701
} E-mail: millers-at-em.agr.ca
}
}
}




From daemon Wed Aug 9 15:35:47 2000



From: Leona Cohen-Gould :      lcgould-at-mail.med.cornell.edu
Date: Wed, 9 Aug 2000 16:20:46 -0400
Subject: Re: Maleate buffer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 11:18 AM -0600 8/9/00, Wright, John D. wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

******************
Simply put, UA does not ppt in maleate buffer. Phosphates cause hideous
problems, and I've been told that cacodylate is also problematic.
I side-step the whole issue and do my en bloc UA during dehydration (3%UA
in 50% EtOh for 1 hr following an initial short step in 50% EtOh to remove
the buffer. It works for me. Does it raise anyone's hackles? If yes, why?

Lee

Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175





From daemon Wed Aug 9 15:36:41 2000



From: Walck, Scott D. :      walck-at-ppg.com
Date: Wed, 9 Aug 2000 16:22:40 -0400
Subject: RE: TEM of diamond films

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Robin,
If you put it on NaCl, you can float them off. You might be able to deposit
them right onto freshly cleaved mica and float them off. You might also be
able to put them onto either SiO or carbon support films directly.

If the films are too thin, they may not pop off your substrate. I had DLC
films that were about 0.8 um pop off after a few days because of the stress
in them. You might have to cut them down a little to help.

Carbon resists ion milling without some help in the gas. If you are
interested in cross section TEM, then you will need to do low angle ion
milling. I have a paper in MRS TEM book and the effects of gas compositions
on ion milling. You might want to use a combination of Ne and O2.

The small angle cleavage technique worked beautifully on my DLC samples on
Si. The DLC was too thin at the edge for EELS work. I would definitely try
it with this if you put it on Si, GaAs, sapphire, SiC, or glass.


-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)



} -----Original Message-----
} From: "rgriffin-at-eng.uab.edu"-at-sparc5.microscopy.com
} [mailto:"rgriffin-at-eng.uab.edu"-at-sparc5.microscopy.com]
} Sent: Wednesday, August 09, 2000 11:09 AM
} To: microscopy-at-sparc5.microscopy.com
} Subject: TEM of diamond films
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListse} rver/FAQ.html
}
}
} --------------------------------------------------------------
} ---------.
}
}
} I have a student that would like to do TEM of diamond films that are
} prepared by microwave plasma CVD. The thickness of the films can be
} adjusted to make it electron beam transparent. The student
} believes that he
} can flake off pieces of the film.
}
} I'm thinking maybe we could just lay the diamond film on a
} grid and pop it
} into the TEM. Is this a crazy idea? Does anyone have any
} suggestions?
}
} Thanks,
}
} Robin Griffin
}



From daemon Wed Aug 9 16:29:12 2000



From: Mike Dalbey :      dalbey-at-biology.ucsc.edu
Date: Wed, 09 Aug 2000 14:12:41 -0700
Subject: Wild M8 lit wanted

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I would like to have copies of literature on the Wild M8
stereomicroscope and its accessories. Sales literature, price list,
users manual, parts diagrams, service manuals,etc. are all welcome.
Please contact me first to avoid duplication of your generous efforts. I
will glady reimburse copying and mailing costs.


Mike Dalbey
Biology Dept.
University of California
Santa Cruz, CA 95064

831-459-3674



From daemon Wed Aug 9 16:29:13 2000



From: Ahmed B Faik :      abfaik-at-uncc.edu
Date: Wed, 9 Aug 2000 18:06:07 -0400 (EDT)
Subject: Re: TEM of diamond films

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I suspect it would pop straight off if it became charged, i.e almost
immediately you put the beam onto it!

One approach is to make a sticky grid, and put the film onto that.
Three possible methods - 1) dip the grid into a thin solution of epoxy
resin in PPO, allow all solvent to evaporate, place diamond film and
cure epoxy at 60oC
2) Dissolve a pressure-sensitive adhesive from your favourite brand
of sticky tape or carbon tabs. Dip grid, allow to dry, place film.
3) Coat grid with a thermoplastic solution (e.g. polystyrene) and dry.
place diamond film. Bond by melting plastic on a hot-plate.

For a non-adhesive-based method you could try sandwiching the
diamond film between two grids stuck together at one edge with
epoxy, or use a proprietary sandwich grid (obtainable from SPI, Ted
Pella, Polysciences, Agar Scientific etc.)
Chris

} From: "rgriffin-at-eng.uab.edu"-at-sparc5.microscopy.com
To: microscopy-at-sparc5.microscopy.com



somehow you need to glue the diamond film onto the grid, otherwise you
might lose the diamond film inside the TEM.


On Wed, 9 Aug 2000 rgriffin-at-eng.uab.edu-at-sparc5.microscopy.com wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I have a student that would like to do TEM of diamond films that are
} prepared by microwave plasma CVD. The thickness of the films can be
} adjusted to make it electron beam transparent. The student believes that he
} can flake off pieces of the film.
}
} I'm thinking maybe we could just lay the diamond film on a grid and pop it
} into the TEM. Is this a crazy idea? Does anyone have any suggestions?
}
} Thanks,
}
} Robin Griffin
}




From daemon Wed Aug 9 21:47:33 2000



From: =?iso-8859-1?Q?=C1=A4=BF=B5=BF=EC?= :      jyw-at-lgcit.com
Date: Thu, 10 Aug 2000 11:33:10 +0900
Subject: Pore Density Calculation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Microscopist !

I'm interested in calculating pore density using SEM image.
I used several free softwares but couldn't get satisfactory results.
If someone knows software or methods about calculating pore density
using
SEM image, please let me know.

Thanks in advance.

Cheers




From daemon Thu Aug 10 07:12:15 2000



From: Marti, Jordi :      jordi.marti-at-honeywell.com
Date: Thu, 10 Aug 2000 04:55:30 -0700
Subject: TEM of clay in polystyrene

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Robin:

It is not clear from your posting if your sample is a powder or a bulk
specimen. The 1 nm you mention, I assume is this the clay thickness ?

We have looked at different clay materials in a polymer matrix . We normally
tended to section these in a microtome (actually a cryo microtome to
minimize deformation of the matrix.). We tend to use 200KeV for most of our
work, but 100KeV should be OK also. The key here is to get nice thin
sections because the contrast of the very thin clay particles will be better
if the sections are thin. Also , could you share with me the paper
(reference )you mentioned ? . We don't use a cooling holder for our
samples, and contamination has not been a problem for us but then we were
not doing diffraction on individual particles, we were only looking at the
dispersion of the clay.

There is another possibility also which I would like to try when I get a
chance. This would involve etching (plasma?) the surface of the sample to
expose some of the clay and then looking at the sample either by AFM or by
TEM (replicas of the etched surface). This should work on film or bulk
specimens (with the clay in suspension), but I am not sure it would work OK
on powder samples.

I hope this helps,

Jordi Marti
Honeywell

-----Original Message-----
} From: "rgriffin-at-eng.uab.edu"-at-sparc5.microscopy.com
[mailto:"rgriffin-at-eng.uab.edu"-at-sparc5.microscopy.com]
Sent: Wednesday, August 09, 2000 3:25 PM
To: microscopy-at-sparc5.microscopy.com


I have a student who would like to do TEM on samples of clay in polystyrene.
The particles are very small (around 1 nm in thickness) and are in
suspension are in suspension. He believes that we could scoop up some
sample onto a carbon film and examine them in an SEM. He has a chemistry
paper where someone did just that. I have a TEM that has a voltage range of
100-200kV. I have never worked on polymers or a clay.

Questions:
Will clay or polystyrene contaminate the TEM? What voltage range is safe?
Any tricks?

By the way I do have a stage that can be liquid nitrogen cooled to minimize
radiation damage.

Thanks for any input.

Robin at UAB



From daemon Thu Aug 10 07:43:09 2000



From: Andrea Weisberg :      AWeisberg-at-niaid.nih.gov
Date: Thu, 10 Aug 2000 08:29:45 -0400
Subject: M&M Meeting in Phila

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


HI All,

There will be a special symposium which is dedicated to Dr. Lee
Peachey, a well respected scientist and microscopist. The Symposium is
number 16A on Tuesday, August 15th in Room 108.
The Other Motor city:
Muscle and Non-muscle Motility
A Dedication to Dr. Lee Peachey

Dr. Lee Peachey uses confocal microscopy and High Voltage EM to
study 3-D structural information on cell structures.
The line up for the session is terrific. Dr. Saul Winegrad will
chair the morning session and Dr. Clara Frazini-Armstrong will chair the
afternoon session.
All of the presenters are the tops in there fields. One highlight
is
Sir Andrew Huxley from Trinity College, Cambridge will give a talk on
Microscopy of Muscle in the 19th and 20th Centuries. Dr. Huxley received the
Nobel Prize in Medicine in 1963 for discoveries concerning the ionic
mechanisms involved in excitation and inhibition in the peripheral and
contrail portions of the nerve cell membrane.

Don't miss this session,
Regards, Andy


Andrea S. Weisberg
NIH/NIAID/LVD
Bldg. 4/Room 210
4 Center Drive
Bethesda, MD 20892-0445
phone: (301) 435-1977
fax: (301) 480-1147
e-mail: aweisberg-at-nih.gov



From daemon Thu Aug 10 08:35:51 2000



From: Garry Burgess :      GBurgess-at-exchange.hsc.mb.ca
Date: Thu, 10 Aug 2000 08:23:04 -0500
Subject: FW: TEM/Wrinkled Spurr Sections

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I wondered if you tried baking the grids and sections in a drying oven at 70
degrees C for 10 minutes or so? This will ensure that the sections will not
come off the grids in any way, if that is what is happening here. (since you
report that you don't seem to have this problem on unstained sections, it
seems to me that your sections might be lifting a bit from the grid, causing
wrinkling) If you leave the sections in the oven longer, or even overnight,
I've noticed that it doesn't seem to harm them in any way. Once the
sections are baked onto the grids in this manner, even a firehose directed
at full spray cannot remove the sections. I might also add that heating the
sections in this manner does not affect them adversely in staining. It
might be the quick fix that you are looking for.

Garry


} ----------
} From: Tamara Howard[SMTP:howard-at-cshl.org]
} Sent: Tuesday, August 08, 2000 2:29 PM
} To: Anthony Greco
} Cc: Microscopy-at-sparc5.microscopy.com
} Subject: Re: TEM/Wrinkled Spurr Sections
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Have you tried aqueous stains for the Spurr's? I've used aq. UA/Pb and had
} nice staining. If you have some stain-resistant plant material, you can
} use KMnO4 instead of UA - it stains well.
}
} Tamara Howard
} CSHL
}
}
} On Tue, 8 Aug 2000, Anthony Greco wrote:
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } I have a constant problem of regularly spaced wrinkles on my Spurr
} } sections on the TEM. I stain with uranyl acetate in 50% ethanol for
} } about 30 minutes followed by stable lead for 15 minutes.(Wrinkling is
} } even worse with methanolic UA). I am certain the problem has something
} } to do with staining since few wrinkles appear when I observe unstained
} } sections. I also rarely encounter wrinkled sections with Embed 812
} } sections when using aqueous UA stains. Any suggestions?
} }
} } Tony Greco
} }
} }
} }
}
}




From daemon Thu Aug 10 14:13:44 2000



From: Donald O'Leary :      donoleary-at-worldnet.att.net
Date: Thu, 10 Aug 2000 14:50:54 -0400
Subject: LM Workshop "Use of the Microscope"

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


New York Microscopical Society
1244 McBride Avenue
West Paterson, NJ 07424


Bernard Friedman Memorial Workshop


Use of the Microscope
September 16, 23, 30, October 7, 2000

A basic course on light microscopy which will cover the following topics:
Theory of microscopy
Kohler Illumination
Diffraction Theory
Contrast Methods
Polarized light
Phase Contrast
Interference
. . . . Hoffman contrast, Rheinberg, Dark-field & oblique Illumination,
etc.

The workshop will consist of four consecutive Saturdays of lectures and
hands on labs to cover the theoretical and practical aspects of microscopy.
The course instructors include Jan Hinsch of Leica, Inc., Dennis O’Leary of
Micro-Optical Methods, Mary McCann of McCann Imaging, John Reffner of SensIR
Technologies, Inc. and N.Y.M.S. Instructor Don O'Leary.

WHEN: September 16, 23,30, October 7, 2000 from 10 A.M. to 4 P.M.

WHERE: 1244 McBride Avenue, West Paterson, NJ. Phone (973) 812-8377 (Free
parking, accessible by public transportation, Information on car pools and
transportation will be provided.)

COST: $275 for N.Y.M.S. members, $295 for non-members (includes membership)
Lunch and course materials are included. Checks made out to N.Y.M.S.

WHO: Beginners and experienced users who wish to learn more about the
proper use of a microscope.

HOW: Register using the form below. Limited to the first 12 registrants.
Send form to Don O'Leary, 6 Chittenden Road, Fair Lawn, NJ 07410.

FURTHER INFORMATION: Call D. O'Leary (201) 797 -8849
E-mail donoleary-at-worldnet.att.net Fax (425) 988-1415

PLEASE POST
----------------------------------------------------------------------------
-------------------
Registration Form
Use of the Microscope

N.Y.M.S. Member_________________ ($275) Non-Member__________($295)

Name______________________________________________________________________
Address____________________________________________________________________
Phone (W)_______________________(H)___________________________




From daemon Thu Aug 10 15:02:21 2000



From: Geoff McAuliffe :      mcauliff-at-UMDNJ.EDU
Date: Thu, 10 Aug 2000 15:47:35 -0400
Subject: Re: Maleate buffer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Leona Cohen-Gould wrote:

} At 11:18 AM -0600 8/9/00, Wright, John D. wrote:
} } }
} } Off-and-on since the 1970s I've been preparing uranyl acetate for en bloc
} } staining using maleate buffer at pH 5.2 adjusted with NaOH. However, the
} } pKa I for maleic acid is 2.0 and the general rule for buffer selection is
} } that the the desired pH should be within +/- one pH unit of the pKa value
} } (p. 46 in Buffer Solutions,The Basics by Beynon and Easterby; IRL Press,
} } 1996). Therefore, maleate buffer appears not to have a good buffering
} } capacity at pH 5.2. Does anyone understand why maleate was chosen as
} } bufffer for this procedure, and what I may be missing in my thinking on this
} } subject? A current reference for this en bloc staining technique is found
} } in Biomedical Electron Microscopy by Maunsbach and Afzelius, Academic Press,
} } 1999.
} }
} } /John/
} }
} } John D. Wright, Ph.D.
} } West Desert Test Center
} } Dugway, UT
}
} ******************
} Simply put, UA does not ppt in maleate buffer. Phosphates cause hideous
} problems, and I've been told that cacodylate is also problematic.
} I side-step the whole issue and do my en bloc UA during dehydration (3%UA
} in 50% EtOh for 1 hr following an initial short step in 50% EtOh to remove
} the buffer. It works for me. Does it raise anyone's hackles? If yes, why?
}
} Lee
}
} Leona Cohen-Gould, M.S.
} Sr. Staff Associate
} Director, Electron Microscopy Core Facility
} Manager, Optical Microscopy Core Facility
} Joan & Sanford I. Weill Medical College
} of Cornell University
} voice (212)746-6146
} fax (212)746-8175

Well, my hackels are not up but I have read that long (more than a few hours)
exposure to ethanol will extract cytoplasmic material even after glut. and
osmium. I do agree that UA will give terrible ppt. with phosphate and I think
cacodylate as well. Many rinses are needed to get the salts out. I have read that
UA in distilled water works well for en bloc staining. Maleate buffer always
seemed like a pain to me.

Geoff
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************





From daemon Thu Aug 10 16:02:06 2000



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Thu, 10 Aug 2000 10:45:27 -1000 (HST)
Subject: Re: TEM: yellow PbCitrate

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On Mon, 7 Aug 2000, Margaret Brannigan wrote:

} Does anyone know why freshly made lead citrate sometimes comes out
} yellow, and what adverse effects, if any, result? In going over
} past threads on the subject, it seems most people have a relatively
} colorless product as the end result, but a few report the yellow
} color as normal. After years of making up "colorless" lead citrate,
} mine is now yellow. I've toyed with NaOH sources and pH; I use
} ultra pure water, I've cleaned glasswear 'till it squeaks...no
} change....it's still yellow.
}
} Sections seem to stain okay, although the stain goes bad very
} quickly, sometimes even on the grid itself. Since this can be a
} problem with colorless lead citrate, I'm not sure the yellowing is
} the cause. I'd sure appreciate any feedback!
}
This happened to me a year ago, and I was equally puzzled. First I
thought it was a reaction with the rubber on the plunger of the 10 ml
syringe in which I store my stain (with a filter on the end it makes a
great drop dispenser). I had opened a new box of *old* syringes. So I
changed back to my old *new* syringes, but the newly made stain was still
yellow and short lived. Same water, temperture, glassware, balance,
etc. as my usual stuff. I suspected the distllled water source.

The next day a woman who has been doing TEM for ca. 35 years came in to
ask if I'd ever seen yellow lead citrate. She had just goten this for
the first time. She works in a different lab in a diffrent building with
different water and, in fact, there should be absolutely no correlation at
all. Except for phase of the moon, which theory we briefly entertained.

A few days later I successfully made up clear lead citrate. The main
change I had made was to make up fresh 10N NaOH. However, the phase of
the moon had changed as well.

Good luck!

Tina

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************




From daemon Thu Aug 10 18:13:32 2000



From: Mark Riggs :      riggsm-at-svg.com
Date: Thu, 10 Aug 2000 17:54:42 -0500
Subject: aluminum oxide slurry analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



vendor-supplied slurry is used in lens polishing. recent polishings have
resulted in scratches on lens. i have been asked to provide data with my
xl40feg regarding particle sizes. am i correct in assuming that particle
size distribution (in the slurry environment) is as important a criteria as
simple particle diameters? can i evaluate simple particle size by "boiling
off" the suspension, and sprinkling conductive tape with the remaining
powder? can i "fire" the slurry into a ceramic brick, and polish the
result for slurry particle distribution analysis? what would the procedure
entail? thanks.

mark riggs
svgl
wilton, ct





From daemon Thu Aug 10 18:20:16 2000



From: PersHlth :      pershlth-at-ci.amarillo.tx.us
Date: Thu, 10 Aug 2000 18:04:58 -0500
Subject: storage of vaccine and blood

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Can any one assist me in locating any resources related to the storage
of vaccine and blood/or urine in freezers (together). Terry Arnold





From daemon Thu Aug 10 19:33:32 2000



From: Nathan Haese :      nathan_haese-at-CompuServe.COM
Date: Thu, 10 Aug 2000 20:11:17 -0400
Subject: storage of vaccine and blood

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Terry,

You could try the website for the American Association of Blood Banks,
www.aabb.org. It is a trade organization. They may have technical leads
and links.

Nathan Haese



From daemon Fri Aug 11 07:10:35 2000



From: =?euc-kr?B?uq/BpLz2?= :      jsbyun-at-snumfc.snu.ac.kr
Date: Mon, 12 Jun 2000 20:54:31 +0900
Subject: about wedge polisher(Tripod)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

I'm researching about the microstructure of steel,
and have been usually using zet polishing and ion milling.
Now I'm considering wedge polisher (Tripod) as a TEM sample preparation method,
but rarely know about this.

I'd like to know whether this method can be used generally ,compared with zet polishing
and ion milling. And I'd like to know advantages and disadvantages.
Also, would you let me know the companies that produce wedge polisher (Tripod)?

Thanks in advace.

JungSoo.

Jung-Soo Byun
School of Materials Science & Engineering
College of Enineering, Seoul National University
Seoul 151-742, Korea
Tel : +82-2-880-7100, Fax : +82-2-885-9671
H.P. : 016-226-4358



From daemon Fri Aug 11 07:17:10 2000



From: White, Woody N :      Woody.N.White-at-mcdermott.com
Date: Fri, 11 Aug 2000 07:51:00 -0500
Subject: Re:aluminum oxide slurry analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Mark,

You might consider filtering the slurry, rinsing the filtrate well.
Choice of filter media is important. For you application, I would
suggest a membrane filter rather than a fiberous one.
..Then dry, coat, and examine on filter -or apply filtrate to carbon tape.

Many slurrys contain more than just water and abrasive.
Lubricants, suspention agents , etc. can be present which may coat
the particulate if evaporative techniques are used alone.

As for firing - no. Under the correct (wrong) conditions, you may not just
sinter the powder, but grain size and aglomerations may well be affected.

Woody White
McDermott Technology Inc.

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America



vendor-supplied slurry is used in lens polishing. recent polishings have
resulted in scratches on lens. i have been asked to provide data with my
xl40feg regarding particle sizes. am i correct in assuming that particle
size distribution (in the slurry environment) is as important a criteria as
simple particle diameters? can i evaluate simple particle size by "boiling
off" the suspension, and sprinkling conductive tape with the remaining
powder? can i "fire" the slurry into a ceramic brick, and polish the
result for slurry particle distribution analysis? what would the procedure
entail? thanks.

mark riggs
svgl
wilton, ct



From daemon Fri Aug 11 07:46:10 2000



From: Ni, Chao-Ying :      CYNi-at-rodel.com
Date: Fri, 11 Aug 2000 08:33:38 -0400
Subject: aluminum oxide slurry analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Mark,

I dont understand what is meant by x140feg. Is that an SEM? We do slurry
analysis on daily basis using a TEM. Sample preparation includes to dilute
slurry to about 1%vol and to follow a standard "drop grid" procedure. Please
let me know if you have any further questions.

Have a nice day!

Chao Ni, PhD
Rodel Inc.
451 Bellevue Road
Newark, DE 19713
(302) 366-0500 ext 2812


-----Original Message-----
} From: Mark Riggs [mailto:riggsm-at-svg.com]
Sent: Thursday, August 10, 2000 6:55 PM
To: Microscopy-at-sparc5.microscopy.com



vendor-supplied slurry is used in lens polishing. recent polishings have
resulted in scratches on lens. i have been asked to provide data with my
xl40feg regarding particle sizes. am i correct in assuming that particle
size distribution (in the slurry environment) is as important a criteria as
simple particle diameters? can i evaluate simple particle size by "boiling
off" the suspension, and sprinkling conductive tape with the remaining
powder? can i "fire" the slurry into a ceramic brick, and polish the
result for slurry particle distribution analysis? what would the procedure
entail? thanks.

mark riggs
svgl
wilton, ct





From daemon Fri Aug 11 08:39:20 2000



From: Ron_Kneisler-at-albemarle.com
Date: Fri, 11 Aug 2000 08:25:35 -0500
Subject: Looking for Carbon Coater for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Our old coater is on it's last legs so I'm looking to purchase a new carbon
coater for our FESEM for EDS work. Since I inherited the old coater 'm looking
for any advice (and good or bad experiences) with carbon coaters from the
various manufacturers. The unit should be a bench top unit, user friendly, and
as automatic as possible since it will be operated by several users.

Thanks in advance,
Ron Kneisler
Albemarle Corporation
ron_kneisler-at-albemarle.com
******************************************************************************************
IMPORTANT NOTICE: This email message and any files transmitted with it may be
confidential, may be legally privileged, and are for the intended recipient
only. If obtained in error, please delete this message and confirm the deletion
in an email to the sender.
******************************************************************************************





From daemon Fri Aug 11 09:30:25 2000



From: Shea Miller :      millers-at-em.agr.ca
Date: Fri, 11 Aug 2000 10:21:53 -0400
Subject: in situ hybridization

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello everyone;
after lurking for months, I have my second question in a week. Thanks to everyone who replied to my question re: fixation for enzyme histochemistry... we have decided to go with a short fixation in 1.75% glutaraldedhyde in buffer, and are keeping our fingers crossed (Kristin... I will reply in more detail to you).

Another grad student is wanting to do some in situ hybridizations on soybean and soybean seed coat. She would like to know if you can use lyophilized material.... I have only used stuff that was harvested fresh, fixed and processed through paraplast using a normal solvent dehydration, so I cannot advise her here. Any advice would be most helpful.

thanks in advance
shea


Dr. S.Shea Miller
Agriculture & AgriFood Canada
Eastern Cereal and Oilseed Research Centre
Rm. 2068 Neatby Building
Central Experimental Farm
Ottawa, Ontario
Canada K1A 0C6
Phone: (613) 759-1760
Fax: (613) 759-1701
E-mail: millers-at-em.agr.ca




From daemon Fri Aug 11 09:58:31 2000



From: adriana-at-cena.usp.br
Date: Fri, 11 Aug 2000 11:42:03 -0300
Subject: website of SBMM?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Would anyone have the web site of the "Sociedade Brasileira de Microscopia
e Microanalise - SBMM"?
Thanks in advance,

Adriana

Adriana Pinheiro Martinelli Rodriguez, PhD
Laboratorio de Biotecnologia Vegetal
CENA, Universidade de Sao Paulo
Av. Centenário 303, Cx. Postal 96
13400-970, Piracicaba, SP, Brasil
phone: +55-19- 429-4694
fax: +55-19- 429-4610
adriana-at-cena.usp.br
http://www.cena.usp.br/labs/labbiotecveg.htm







From daemon Fri Aug 11 10:05:54 2000



From: Julie Gross :      jgross-at-neuron.uchc.edu
Date: Fri, 11 Aug 2000 10:55:00 -0400
Subject: Microscopes for High School

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am trying to help a Hartford, CT high school biology teacher find
microscopes for her classes. Any leads, ideas, advice for locating
microscopes? I'm in Connecticut but welcome input from anywhere.

Julie Gross
Dept. of Neuroscience
University of CT Health Center
Farmington Ave.
Farmington, CT 06030
jgross-at-neuron.uchc.edu




From daemon Fri Aug 11 10:20:05 2000



From: Nestor J. Zaluzec :      zaluzec-at-aaem.amc.anl.gov
Date: Fri, 11 Aug 2000 10:08:48 -0500
Subject: Re: Microscopes for High School

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Julie

You should look at the ProjectMicro Pages
on the MSA WWW Site. There is a lot of information
contained therein.

http://www.msa.microscopy.com/ProjectMicro

Caroline Schooley is the best contact for additional
details/help. Her contact info is on the above page.

Nestor
Your Friendly Neighborhood SysOp




} X-Sender: jgross-at-neuron.uchc.edu
} Date: Fri, 11 Aug 2000 10:55:00 -0400
} To: MSA Listserver {Microscopy-at-sparc5.microscopy.com}
} From: Julie Gross {jgross-at-neuron.uchc.edu}
} Subject: Microscopes for High School
} Mime-Version: 1.0
} Status:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

==================================================================
Nestor J. Zaluzec
Materials Science Division
Building 212
Argonne National Lab
9700 S. Cass Ave
Argonne, Illinois 60439 USA
Tel: 630-252-7901, Fax: 630-252-4798
Email: Zaluzec-at-aaem.amc.anl.gov
==================================================================
TPMLab: http://tpm.amc.anl.gov
MMSite: http://www.amc.anl.gov
==================================================================

The box said "This program requires Win 95/98/NT or better..." so I bought
a G3 Mac

==================================================================





From daemon Fri Aug 11 10:36:08 2000



From: Mandayam V. Parthasarathy :      mvp2-at-cornell.edu
Date: Fri, 11 Aug 2000 11:31:55 -0400
Subject: Re: Looking for Carbon Coater for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


More useful information.

Bruce F. Ingber, Biologist
USDA-ARS, SRRC
1100 Robert E. Lee Blvd.
New Orleans, LA 70124
(504) 286-4270 phone
(504) 286-4419 fax
bingber-at-commserver.srrc.usda.gov

-----Original Message-----
} From: Ringnalda, Jan [mailto:JRingnalda-at-FEICO.com]
Sent: Friday, August 11, 2000 9:47 AM
To: 'Ingber, Bruce F.'; Long, Jo; Piscopo, Irene
Cc: Schaub, Daniel; Rice, Trisha (CS); Booth, Steve;
'sherman-at-btny.purdue.edu'


Ron,

We have a Bal-Tec SCD 050 Coater (6+ yrs. old) with two separate heads for
carbon sputtering and gold/palladium sputtering respectively. We use
carbon threads for sputtering. It has been very satisfactory for our
FESEM work. The Bal-Tec unit is quite user-friendly.

------------------------------------------------------------
} Our old coater is on it's last legs so I'm looking to purchase a new carbon
} coater for our FESEM for EDS work. Since I inherited the old coater 'm looking
} for any advice (and good or bad experiences) with carbon coaters from the
} various manufacturers. The unit should be a bench top unit, user friendly, and
} as automatic as possible since it will be operated by several users.
}
} Thanks in advance,
} Ron Kneisler
} Albemarle Corporation
} ron_kneisler-at-albemarle.com

*******************************************************************

Dr. M.V. Parthasarathy
Prof. of Plant Biology, Adjunct Prof. of Biomedical Sciences (Vet), &
Director, Cornell Integrated Microscopy Center (CIMC)
Department of Plant Biology
228 Plant Science Building
Cornell University, Ithaca, NY 14853
E-Mail: mvp2-at-cornell.edu
Plant Biology Office: 268 Emerson; Telephone: 607-255-1734
Plant Biology Fax: 607-255-5407
CIMC Office: C1 054 Vet. Med. Center; Telephone: 607-253-3803
CIMC Office Fax: 607-253-3803
CIMC web site: http://www.cimc.cornell.edu





From daemon Fri Aug 11 11:18:51 2000



From: David Henriks :      Henriks-at-CompuServe.COM
Date: Fri, 11 Aug 2000 12:05:44 -0400
Subject: Looking for Carbon Coater for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ron:

As you are doing FESEM work, have you considered a high resolution
deposition system? Our IBS/e Ion Beam Sputter Deposition and Etching
System can act as a carbon "coater", but it can also deposit chromium,
iridium etc. Using ion beam deposition allows you to controllably deposit
very thin, extremely uniform films without heating the sample. Just a
thought. Let me know if you would like additional information.

DISCLAIMER: South Bay Technology produces equipment and supplies as
described above and, therefore, has a vested interest in promoting their
use.

Best regards-

David
Writing at 7:25:03 AM on 08/11/2000

***************************************************************************
************************

David Henriks
Vice President TEL: 800-728-2233
(toll free in the USA)
South Bay Technology, Inc. +1-949-492-2600
1120 Via Callejon FAX: +1-949-492-1499
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

***************************************************************************
************************

} } } } } Please visit us at http://www.southbaytech.com { { { { {

Manufacturers of precision sample preparation equipment and supplies for
metallography, crystallography and electron microscopy.

Message text written by
INTERNET:"Ron_Kneisler-at-albemarle.com"-at-sparc5.microscopy.com
}
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America




Our old coater is on it's last legs so I'm looking to purchase a new carbon
coater for our FESEM for EDS work. Since I inherited the old coater 'm
looking
for any advice (and good or bad experiences) with carbon coaters from the
various manufacturers. The unit should be a bench top unit, user friendly,
and
as automatic as possible since it will be operated by several users.

Thanks in advance,
Ron Kneisler
Albemarle Corporation
ron_kneisler-at-albemarle.com {




From daemon Fri Aug 11 11:18:51 2000



From: David Henriks :      Henriks-at-CompuServe.COM
Date: Fri, 11 Aug 2000 12:05:56 -0400
Subject: about wedge polisher(Tripod)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Jung-Soo:

South Bay Technology, Inc. is the manufacturer of the Tripod Polisher which
is based on original work performed at the IBM East Fishkill facility
(Anderson, Klepeis, Benedict et al). The Tripod Polisher, using the wedge
polishing technique, can be used for a variety of materials. It has been
used successfully for thinning semiconductors from silicon to indium
phosphide, metals, ceramics, geological samples, hard disk media,
composites etc. When used properly, some materials can be mechanically
polished to electron transparency without the need for ion milling.
Typically, a short ion milling step is required.

Jet polishing, in general, is a great method for preparing homogeneous
materials if you do not have a specific area of interest. Jet polishing is
a quick, effective and damage free method of preparing specimens. Of
course, with a single jet polisher, you can control the area within the
sample that you want to reach, but even at that, cannot very easily pick a
specific spot. The Tripod Polisher provides a means to thin to a very
specific area - in the case of semiconductors even to a specific sub-micron
device. The main advantage of the Tripod Polisher is control. Tripod
Polishing is an iterative process of polishing and visually inspecting your
sample. Certain parts of the process can be automated - such as the first
side polish (essentially an SEM cross section) by simply placing the Tripod
on the yoke of a polishing machine. This is a nice way to do it as you can
easily lift the polisher off for visual inspection and replace it within
seconds. If alignment is done properly prior to 1st side polishing, first
side visual inspection is minimal. The second side polish is more critical
as that is where you are trying to locate your specific area of interest
and progress the wedge in such a way as to maximize the thin area. Ongoing
visual inspection is critical on the 2nd side or "wedge" polishing step.
2nd side polishing is generally done by hand ( although Allied High Tech
sells a system that they say automates the whole process) as this allows
you the most control and is the quickest way to change between the
polishing and visual inspection.

The polishing is done using diamond abrasive films in a series of steps.
The amount of time that you spend on any one polishing step is very short
so it is important that you can quickly remove the Tripod Polisher and the
diamond film to move on to the next step. This is important for a few
reasons, but one of the most important is that the material you are
removing at these final steps is so small, that you can over polish very
quickly if you are not careful.

We have been doing Tripod Polishing here for over 10 years and have a huge
base of customers as well as an extensive library of technical papers and
application notes. Also, we do conduct ongoing courses in TEM specimen
preparation including a specialized course in Tripod Polishing. I would
be pleased to send you more detailed information if you have an interest.
I hope this helps.

DISCLAIMER: South Bay Technology produces equipment and supplies as
described above and, therefore, has a vested interest in promoting their
use.

Best regards-

David
Writing at 8:14:26 AM on 08/11/2000

***************************************************************************
************************

David Henriks
Vice President TEL: 800-728-2233
(toll free in the USA)
South Bay Technology, Inc. +1-949-492-2600
1120 Via Callejon FAX: +1-949-492-1499
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

***************************************************************************
************************

} } } } } Please visit us at http://www.southbaytech.com { { { { {

Manufacturers of precision sample preparation equipment and supplies for
metallography, crystallography and electron microscopy.

Message text written by "=?euc-kr?B?uq/BpLz2?="
}
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Hi,

I'm researching about the microstructure of steel,
and have been usually using zet polishing and ion milling.
Now I'm considering wedge polisher (Tripod) as a TEM sample preparation
method,
but rarely know about this.

I'd like to know whether this method can be used generally ,compared with
zet polishing
and ion milling. And I'd like to know advantages and disadvantages.
Also, would you let me know the companies that produce wedge polisher
(Tripod)?

Thanks in advace.

JungSoo.

Jung-Soo Byun
School of Materials Science & Engineering
College of Enineering, Seoul National University
Seoul 151-742, Korea
Tel : +82-2-880-7100, Fax : +82-2-885-9671
H.P. : 016-226-4358

{




From daemon Fri Aug 11 11:23:54 2000



From: HILDEGARD CROWLEY :      hcrowley-at-du.edu
Date: Fri, 11 Aug 2000 10:12:26 -0600 (MDT)
Subject: LM stain for DAB epoxy sections??

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Dear friends,

We need a nuclear LM stain for tissue sections (3-4 microns thickness)
which have been processed with DAB label and embedded in epoxy. Does
anyone have such a method? We would much appreciate any ideas.

Thanks,

Hildegard H. Crowley
University of Denver






From daemon Fri Aug 11 11:30:38 2000



From: Ladd Research :      ladres-at-worldnet.att.net
Date: Fri, 11 Aug 2000 12:19:53 -0400
Subject: M&M 2000 Symposium

Contents Retrieved from Microscopy Listserver Archives
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We have received many questions concerning the use of Mercox for
corrosion casting recently, but to provide first hand experience to
those people and any others who may have an interest, we are once again
sponsering the Vascular Corrosion Casting symposium at the M&M meeting
next week in Philadelhia.
This symposium will once again be chaired by Dr. Hossler, Dr.
Aharinejad, and Dr. Lametschwandtner. It will be held Wed., Aug 16 at
8:30 am in room C122. I would urge all those who are interested to
attend.

John Arnott
Chairman
--

LADD RESEARCH
131 Dorset Lane
Williston, VT 05495 USA

TEL 1-800-451-3406 (US) or 1-802-878-6711 (anywhere)
FAX 1-802-878-8074
e-mail ladres-at-worldnet.att.net

ALL NEW WEB SITE UP NOW AT OUR NEW URL:

http://www.laddresearch.com



From daemon Fri Aug 11 12:24:34 2000



From: Christoph Bauer :      cbauer-at-midway.uchicago.edu
Date: Fri, 11 Aug 2000 12:08:22 -0700
Subject: Re: Maleate buffer

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uranyl acetate results in giving precipitates in our hands with either
PBS or
sodium cacodylate. For cell cultures we do the following: After osmium
we do
several washes with water and do the en bloc staining with 1% UA in
water for 1h
in the dark. We usally make a 4% UA solution and check the pH with paper
before
use. It should be between 4 and 5. If not we discard the solution.

Christoph Bauer
University of Chicago



"Wright, John D." wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Off-and-on since the 1970s I've been preparing uranyl acetate for en bloc
} staining using maleate buffer at pH 5.2 adjusted with NaOH. However, the
} pKa I for maleic acid is 2.0 and the general rule for buffer selection is
} that the the desired pH should be within +/- one pH unit of the pKa value
} (p. 46 in Buffer Solutions,The Basics by Beynon and Easterby; IRL Press,
} 1996). Therefore, maleate buffer appears not to have a good buffering
} capacity at pH 5.2. Does anyone understand why maleate was chosen as
} bufffer for this procedure, and what I may be missing in my thinking on this
} subject? A current reference for this en bloc staining technique is found
} in Biomedical Electron Microscopy by Maunsbach and Afzelius, Academic Press,
} 1999.
}
} /John/
}
} John D. Wright, Ph.D.
} West Desert Test Center
} Dugway, UT

--
MZ



From daemon Fri Aug 11 13:31:56 2000



From: Philippe-Andre Buffat :      philippe.buffat-at-epfl.ch
Date: Fri, 11 Aug 2000 10:28:15 +0200
Subject: Re: TEM of diamond films

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If your substrate is brittle, you can also cleave it to make a small
slab that you glue on a TEM grid. If the substrate is too thick, you
may need to pre-thin it by mechanical polishing.

Then look with the TEM along the cleaved edge at about 45° tilt.

It's fast, it's clean, it does not require any complex tools, but of
course the the transparent area is quite small!

Best regards

Philippe Buffat

}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } I have a student that would like to do TEM of diamond films that are
} } prepared by microwave plasma CVD. The thickness of the films can be
} } adjusted to make it electron beam transparent. The student
} believes that he
} } can flake off pieces of the film.
} }
} } I'm thinking maybe we could just lay the diamond film on a grid and pop it
} } into the TEM. Is this a crazy idea? Does anyone have any suggestions?
} }
} } Thanks,
} }
} } Robin Griffin
} }
}
}
} ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
} Dr Chris Jeffree
} University of Edinburgh
} Biological Sciences EM Facility
} Daniel Rutherford Building
} King's Buildings EDINBURGH EH9 3JH
} Tel: +44 (0) 131 650 5345
} FAX: +44 (0) 131 650 6563
}
} Inveresk Cottage, 26 Carberry Road,
} Inveresk, Musselburgh, Midlothian EH21 8PR, UK
} Tel: +44 (0) 131 665 6062 / Mobile 07710 585 401
} FAX: +44 (0) 131 653 6248
} ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

__________________________________________________________________
Philippe Buffat
Ecole Polytechnique Federale de Lausanne (EPFL)
Centre Interdepartemental de Microscopie Electronique
Address: EPFL-CIME, Batiment MX-C, CH-1015 Lausanne, Switzerland
Phone: +41(21)693 29 83 Fax: +41(21)693 44 01 (Central European Time)
E-mail: philippe.buffat-at-epfl.ch, WWW URL http://cimewww.epfl.ch/
______________________________ Eudora F2.1 ___________________________



From daemon Fri Aug 11 13:38:03 2000



From: prenitzer,brenda s :      prenitzer-at-lucent.com
Date: Fri, 11 Aug 2000 14:23:57 -0400
Subject: unsubscribe please

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From daemon Fri Aug 11 13:38:28 2000



From: William Tivol :      tivol-at-wadsworth.org
Date: Fri, 11 Aug 2000 14:20:01 -0400
Subject: Re: TEM: yellow PbCitrate

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Tina Carvalho wrote:

}
} On Mon, 7 Aug 2000, Margaret Brannigan wrote:
}
} } Does anyone know why freshly made lead citrate sometimes comes out
} } yellow, and what adverse effects, if any, result?
} }
} } Sections seem to stain okay, although the stain goes bad very
} } quickly, sometimes even on the grid itself. Since this can be a
} } problem with colorless lead citrate, I'm not sure the yellowing is
} } the cause. I'd sure appreciate any feedback!
} }

[skip]

}
} A few days later I successfully made up clear lead citrate. The main
} change I had made was to make up fresh 10N NaOH. However, the phase of
} the moon had changed as well.

Dear Margaret and Tina,
Is it possible that CO2 reacted with the old NaOH? Is PbCO3 yellow?
That would explain why fresh NaOH would work.
Yours,
Bill Tivol




From daemon Fri Aug 11 14:33:20 2000



From: Greg Strout :      gstrout-at-ou.edu
Date: Fri, 11 Aug 2000 14:12:35 -0500
Subject: Re: Microscopes for High School

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Julie,
You may want to look into the possibility that a local university or
business has some older scopes that they would like to donate for your
efforts.

Our local microscopy society OMS (Oklahoma Microscopy Society) was
graciously granted 128 microscopes that were surplus for the University of
Oklahoma. We sent out notices to the public schools in the state including
an application form for the microscopes. We have since placed all 128
microscopes in a variety of elementary and secondary schools all over
Oklahoma. The microscopes were old, although they were in good shape and
many were fine binocular scopes with 4 objectives and good sub stage
condensers. The lower elementary school grades (k-4) received scopes with 2
objectives and sub stage apertures. These scopes are perfect for the lower
grades because of their simplicity, although they produce nice images and
are very sturdy.

We are always on the lookout for more scopes as the demand from the schools
exceed the supply.
Greg

Julie Gross wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} I am trying to help a Hartford, CT high school biology teacher find
} microscopes for her classes. Any leads, ideas, advice for locating
} microscopes? I'm in Connecticut but welcome input from anywhere.
}
} Julie Gross
} Dept. of Neuroscience
} University of CT Health Center
} Farmington Ave.
} Farmington, CT 06030
} jgross-at-neuron.uchc.edu

--
==================================================================
Greg Strout
Electron Microscopist, University of Oklahoma
WWW Virtual Library for Microscopy:
http://www.ou.edu/research/electron/www-vl/
e-mail: gstrout-at-ou.edu
Opinions expressed herein are mine and not necessarily those of
the University of Oklahoma
==================================================================





From daemon Fri Aug 11 14:54:27 2000



From: Michelle Peiffer :      mlk101-at-psu.edu
Date: Fri, 11 Aug 2000 15:39:11 -0400
Subject: immunolabelling microsomes

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Hi listers,

We have a student working with a protein which is inside microsomes. He
would like us to immunolabel the protein with a polyclonal antibody to
prove that it is inside the microsome and not a contaminant from the
cytoplasm. I would appreciate any suggestions from all who have attempted
this or something similar.

Thanks,


Michelle Peiffer
*************************************************************
Electron Microscope Facility for the Life Sciences
Penn State University Biotechnology Institute
001 South Frear Lab
University Park PA 16802

phone: 814-865-0212
email: mlk101-at-psu.edu
**************************************************************




From daemon Fri Aug 11 16:24:07 2000



From: Gib Ahlstrand :      giba-at-puccini.cdl.umn.edu
Date: Fri, 11 Aug 2000 16:18:50 -0500 (CDT)
Subject: Re: website of SBMM?

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Adriana,

In the Microscopy & Microanalysis journal of Microscopy Society of America,
affiliated with Brazilian Society, there are e-mail contacts for SBMM. I suggest
you start with these: sbmm.ceme-at-epm.br OR: microcel-at-biomed.icb2.usp.br

Good luck,

Gib Ahlstrand

P.S. I was just in your area a few weeks ago and enjoyed the nice cool weather!
Its hotter than blazes in Minnesota right now.
--------------------------------------------
Responding to the message of {4.1.20000811114014.009a4820-at-mail.cena.usp.br}
from "adriana-at-cena.usp.br"-at-sparc5.microscopy.com:

} Would anyone have the web site of the "Sociedade Brasileira de Microscopia
} e Microanalise - SBMM"?
} Thanks in advance,
}
} Adriana
}
} Adriana Pinheiro Martinelli Rodriguez, PhD
} Laboratorio de Biotecnologia Vegetal
} CENA, Universidade de Sao Paulo
} Av. Centenário 303, Cx. Postal 96
} 13400-970, Piracicaba, SP, Brasil
} phone: +55-19- 429-4694
} fax: +55-19- 429-4610
} adriana-at-cena.usp.br
} http://www.cena.usp.br/labs/labbiotecveg.htm
}
}
}
}
}
}
} .


Gib Ahlstrand
Electron Optical Facility, University of Minnesota, Dept. Plant Pathology
495 Borlaug Hall, St. Paul, MN. USA. 55108 (612)625-8249
612-625-9728 FAX, giba-at-puccini.cdl.umn.edu
http://biosci.umn.edu/MIC/consortium.html




From daemon Fri Aug 11 16:26:26 2000



From: Alliedhtp-at-aol.com
Date: Fri, 11 Aug 2000 17:13:54 EDT
Subject: Re: about wedge polisher(Tripod)

Contents Retrieved from Microscopy Listserver Archives
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Dear Jung-Soo,

Allied High Tech Products, Inc. has two different devices, the MultiPrep
Precision Polishing System (Part No. 15-2000) and the TEM Wedge Polisher
(Part No. 69-41000). The MultiPrep is an automated tool that is capable of
thinning to samples 1 micron, as well as creating angles to 13 degrees. The
TEM Wedge Polisher is a manual fixturing device that can be ste to remove
material in mmicron incriments.

If you would like more information on this machine you can visit our website
at www.alliedhightech.com or send me an e-mail directly.

I hope this answers your question.

Best Regards,

Armando Verdugo
Laboratory Supervisor
Allied High Tech Products, Inc.
2376 E. Pacifica Place
Rancho Dominguez, CA 90220
(800)675-1118 US and Canada
(310)635-2466 worldwide
(310)762-6808 fax
www.alliedhightech.com



From daemon Fri Aug 11 17:45:45 2000



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Fri, 11 Aug 2000 18:41:00 -0500
Subject: Sociedade Brasileira de Microscopia e Microanálise

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-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

The website address for the Sociedade Brasileira de Microscopia e Microan
lise is at URL
http://venus.rdc.puc-rio.br/sbme2/sbmeport.htm

It still seems to be mostly "under construction" but there is some
information there.

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.spi.cc
########################
============================================





From daemon Sun Aug 13 02:09:26 2000



From: jim :      jim-at-proscitech.com.au
Date: Sun, 13 Aug 2000 16:22:25 +1000
Subject: RE: TEM of starch granules

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It seems the northern hemisphere has collectively gone on leave - or to
Philadelphia. Lets see if we can help, though its decades since I worked on
starch, but those grains in seeds have not changed - I think.

Trying to fix dry seeds is near impossible and you should look at alternative
methods, such as freeze etching or high resolution scanning, perhaps freeze
substitution.
If you can imbibe the tissue for a day or two, its still difficult. It was my
notion that the GA just does not penetrate, regardless of fixation time. You
may have some success by using GA at room temperature or warmer, but I doubt
that.
Using OsO4 you can after part processing actually see under a dissecting scope
how far it has penetrated. Of course OsO4 is little use if you wish to do
cytochemistry.
Suggest that you use the OsO4 also at room and higher temperatures. Plant
enzymes causing autolysis are fairly active in the cold and since in ice
fixation times are perhaps 4x longer than at 20 degrees and maybe 8 times
longer than at 37 degrees (impossible to truly quantify, but this gives the
idea), shorter fixation times at higher temperatures are preferable.
Do a fixation trial, at room temperature in OsO4 for say half, one and two
hours and see what looks best. If the cytoplasm is dark (worse still membranes
turn white) the tissue is overfixed. The granules will always tend to fall out
during sectioning, but if the sections just simply fall apart, its most likely
that the tissue was not fully fixed, infiltration is the less likely cause.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Friday, August 04, 2000 11:22 PM, ABM Siddique
[SMTP:siddiqueabm-at-hotmail.com] wrote:
}
} Dear All:
}
} I have been trying to study the ultra-structure (TEM) of strach granules
} (in wheat and maize seeds) that contains amorphus and crystaline layers.
} Unfortunately, I am unable to look at these layers under TEM even after
} keeping the tissues longer time in fixative (over night in
} 3%gluteraldehyde) and embedding medium (LR white/Spurs for 2 days).
}
} I also tried the the above procedure with isolated starch granules in low
} melted agar but the result is the same - poor penetration of embedding
} resin as a result I am getting broken starch granules without any
} ultrastructural details.
}
} I would appreciate it if anybody could suggest me how to obtaine a
} reasonably good ultra-structural (TEM) details of starch granules.
}
} With thanks,
}
} Siddique
}
}
} Get Your Private, Free E-mail from MSN Hotmail at
} {http://www.hotmail.com/} http://www.hotmail.com
}
}
}




From daemon Sun Aug 13 06:32:00 2000



From: rimachanda :      rimachanda-at-netscapeonline.co.uk
Date: Sun, 13 Aug 2000 12:16:30 +0100
Subject: re: x1 mag or a 1.25 mag objective for CH-2 olympus microscope

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Hi

I am a postgraduate at the department of Engineering at the University
of Sheffield. My samples are transparent glass melts 4cm3 with
inclusions in them. I am using both transmitting light and reflecting
light microscopy.

I would like to obtain a x1 mag or a 1.25 mag, objective for the Olympus
CH-2 microscope, with the following features:
1. The objective lens should have a short barrel i.e. the length of the
objective, from the top [ where it
screws into the objective holder] to the bottom of the objective
[ DT] = {30mm,
-the features of my sample are fairly large so I need an objective
with a short barrel, giving me a
maximum depth of field. This factor is particularly important.
2. At the top where it screws into the objective holder, diameter is
20.11mm
3. with an acromat plan lens corrected for flatness of field
4. the objective will be used without a cover glass and without an
immersion fluid
5. preferably the objective will be infinity corrected. I am not sure
whether a standardized adjustment
length would be necessary in order to allow max depth of focus,
whilst using transmitting
microscopy

At present the objective used on the above microscope is a x 2.5 mag, x5
mag and a x10 mag. They have the following notations
MSPlan x 2.5 mag / 0.07, infinity/ - f = 180, Olympus 100621
MSPlan x 5mag / 0.13, infinity/ - f = 180 , [ this has a short barrel
= 30mm ]

I would welcome any help or advice regarding obtaining the above
objective lens. .


Miss R Chanda
email: R_S_Chanda-at-yahoo.co.uk





From daemon Sun Aug 13 10:51:17 2000



From: Chris Jeffree :      cjeffree-at-srv0.bio.ed.ac.uk
Date: Sun, 13 Aug 2000 16:27:42 +0100
Subject: Fixation and embedding of starch grains

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Biological electron microscopists often fall into the trap of
automatically fixing everything, even when it is inappropriate to do
so. I was once asked what the best fix would be for diatom
frustules, to which the answer is probably to see a doctor!. But
seriously, I am not quite sure why it should be thought necessary to
fix starch grains either with glutaraldehyde or with osmium, or what
bearing such "fixation" would have on starch grains falling out of
epoxy resin sections. Let's look at some fundamentals. Starch
grains are mainly crystalline or paracrystalline assemblages of
glucan. The molecules are therefore in a minimum-energy
configuration, which will be extremely difficult for most other
molecules to enter. Unless this crystalline order is severly disrupted
no significant quantity of epoxy resin will enter the grains -
infiltration is essentially impossible. Glutaraldehyde and osmium will
not react with starch, so it is pointless attempting to fix them unless
there are traces of proteins or lipids within the grain structure that
must be stabilised. I really don't know how much protein or lipid is
present within the crystalline starch - I am sure you'll tell me - but the
main issue here is that the embedding resin is externalized by the
crystalline structure of the grains, and does not bond too well with
the surface either, so that the grain readily separates from the
section. I think the possibility of promoting covalent bonding of the
starch grain surface to the epoxy could be a more promising avenue
to pursue than experimenting with different permutations of fixatives.
There was some discussion in this forum some time ago about
silanes manufactured by Dow Corning that can be used to promote
adhesion of various materials to epoxies and other resins.

Chris Jeffree
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Dr Chris Jeffree
University of Edinburgh
Biological Sciences EM Facility
Daniel Rutherford Building
King's Buildings EDINBURGH EH9 3JH
Tel: +44 (0) 131 650 5345
FAX: +44 (0) 131 650 6563

Inveresk Cottage, 26 Carberry Road,
Inveresk, Musselburgh, Midlothian EH21 8PR, UK
Tel: +44 (0) 131 665 6062 / Mobile 07710 585 401
FAX: +44 (0) 131 653 6248
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~



From daemon Sun Aug 13 14:25:29 2000



From: valerie :      vjouan-at-club-internet.fr
Date: Sun, 13 Aug 2000 21:06:45 +0200
Subject: Arteries fixation

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Dear all,
In my laboratory, we study vascular endothelium with electron microscopy
(Guinea pig). We tried perfusion to fix tissue but endothelium was removed.
We fix them with 4% paraformaldehyde + 0,1 % Gluta + 0,1 % picric ac in
phosphate buffer pH 7,4 overnight. Artery are then embedded on unicryl for
immunocytochemistry. Tissue was stain with uranyl acetate and Reynolds.
With this procedure cell structure isnot very evident, membrane is well
defined but not very contrasted. Ac locate on all the section, not very
locate in a particular place of cells.
We tried other first and second antibodies but it was the same thing.
What can we do?
Do you have any suggestion?
Thanks




From daemon Sun Aug 13 22:59:32 2000



From: Sally Stowe :      STOWE-at-rsbs.anu.edu.au
Date: Mon, 14 Aug 2000 13:41:05 +1000
Subject: SEM Course in Canberra, October

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SEM MASTERCLASS
THE
SEM APPLICATIONS COURSE
3rd to 5th October 2000
THREE DAYS $600 AUS
(Not including meals and accommodation)

An opportunity for a hands-on introduction or a more advanced session,
depending on your requirements, on just about every one of the major
applications of modern scanning electron microscopy:

Conventional SEM - FESEM - cold stage - EDXA- cathodoluminescence -
variable pressure SEM- low kV - coating techniques - film and digital
recording - tricks for difficult specimens.

When? Tues-Thurs 3rd, 4th, 5th October 2000

Where? The Australian National University Electron Microscopy Unit,
Canberra, Australia

Who by? Steve Chapman (Protrain, UK) and ANUEMU staff

Who for? Anyone who feels they could get more out of their time on the
SEM if they had a bit more background and knew more of the possibilities.
Can your machine deliver what you need with a little coaxing? Or would
different equipment make a big difference to your work? For biologists or
materials scientists. Participants are encouraged to bring their own
samples.

What with? FOUR SEMs will be used for the course: a Hitachi 4500 FESEM,
JEOL 6400 SEM, Cambridge 360,
Hitachi 2250-N SEM. Accessories include three EDXA detectors, several cold
stages, and a number of different SE, BSE and CL signal detectors. For
equipment details, see our website, http:/www.anu.edu.au/EMU

Queries: email stowe-at-rsbs.anu.edu.au . Course Registration form also on
website http://online.anu.edu.au/EMU/00chapman.htm


Dr Sally Stowe
Facility Coordinator, ANU EMU
Box 475 Canberra ACT 2601
AUSTRALIA
stowe-at-rsbs.anu.edu.au fax 61 (0)2 6249 3218 or 6279 8525



From daemon Mon Aug 14 03:48:57 2000



From: Robert H. Olley :      r.h.olley-at-reading.ac.uk
Date: Mon, 14 Aug 2000 09:21:28 +0100 (BST)
Subject: TEM / SEM negatives: Epson and Minolta for scanning?

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Does anyone have any experience of either/both of the Epson Expression
1600 Pro or the Minolta Dimage Multi scanners? One thing we would like to
do is to scan negatives at oblique angles, rather than parallel to the
film strip. My understanding is the even medium format scanners constrain
the negative in a holder, whereas using a flat bed with a transmission
attachment (such as the Epson) would allow rotation.

+------------------------------------------------------------------------+
| Robert H.Olley Phone: |
| J.J.Thomson Physical Laboratory {direct line +44 (0) 118 9318572 |
| University of Reading {University internal extension 7867 |
| Whiteknights Fax +44 (0) 118 9750203 |
| Reading RG6 6AF Email: R.H.Olley-at-reading.ac.uk |
| England URL: http://www.reading.ac.uk/~spsolley |
+------------------------------------------------------------------------+





From daemon Mon Aug 14 04:53:25 2000



From: Michael Reiner :      Elektronenmikroskopie-at-web.de
Date: Mon, 14 Aug 2000 11:34:53 +0200
Subject: Re: TEM/Wrinkled Spurr Sections

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Hello Tony!

Here are my two suggestions:

I. Why don´t you try it with 1% aqueous UAc. Either for twenty minutes a room temperature or at 40°C for 7-10 minutes (results in more intense contrast...)

II. For staining with alcoholic UAc we apply the following procedure:
1. 20 min. 1% UAc in 70% alc.
2. wash: 1-2 drops 70% alc.
3. 1-2 drops 50% alc.
4. 3-4 drops aqua dest.
5. dry on filter paper.

You wrinkling problem might be due to a harsh change from alcohol to water, which causes damage to the sections. And, are your sections supported on the grids by a formvar or carbon film?

For Spurr´s resin I would prefer the warm, aquaeous method.
Hope this works.


Greetings,
Michael

}
} I have a constant problem of regularly spaced wrinkles on my Spurr
} sections on the TEM. I stain with uranyl acetate in 50% ethanol for
} about 30 minutes followed by stable lead for 15 minutes.(Wrinkling is
} even worse with methanolic UA). I am certain the problem has something
} to do with staining since few wrinkles appear when I observe unstained
} sections. I also rarely encounter wrinkled sections with Embed 812
} sections when using aqueous UA stains. Any suggestions?
}
} Tony Greco
}
}


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From daemon Mon Aug 14 05:14:38 2000



From: Chris Jeffree :      cjeffree-at-srv0.bio.ed.ac.uk
Date: Mon, 14 Aug 2000 10:57:06 +0000
Subject: scanning prints on lustre paper

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I apologise if this is a little out-of-gamut for this list, but I have been
trying to make digital copies of archival material which only exists
as small prints on lustre paper. On a flat bed scanner, the textured
surface causes objectionable specular reflections which degrade
image quality. Is there a good way to avoid this?

Chris
==========================================
Dr. Chris Jeffree
University of Edinburgh
BIOSEM - Biological Sciences Electron Microscope Facility
Institute of Cell and Molecular Biology
Daniel Rutherford Building
King's Buildings, Mayfield Road
EDINBURGH, EH9 3JH, Scotland, UK
Tel. #44 (0) 131 650 5345
FAX. #44 (0) 131 650 6563
Mobile 07710 585 401
email c.jeffree-at-ed.ac.uk
=========================================



From daemon Mon Aug 14 06:52:39 2000



From: Phillip Rutledge :      prutledge-at-ars.usda.gov
Date: Mon, 14 Aug 2000 05:17:17 -0600
Subject: subscription

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Are there problems with the system or did I somehow get deleted from the system? I haven't received any mail since last Wednesday (8/9/00). If I got deleted
please re-subscribe me. Thank you.

prutledge-at-ars.usda.gov

Phil Rutledge
USDA/ARS
151 Dixon Drive
Chestertown, MD 21620
voice: 410.778.4136 or 2120
fax: 410.778.4399



From daemon Mon Aug 14 08:28:41 2000



From: William Tivol :      tivol-at-wadsworth.org
Date: Mon, 14 Aug 2000 09:07:21 -0400
Subject: Re: scanning prints on lustre paper

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Chris Jeffree wrote:

} I apologise if this is a little out-of-gamut for this list, but I have been
} trying to make digital copies of archival material which only exists
} as small prints on lustre paper. On a flat bed scanner, the textured
} surface causes objectionable specular reflections which degrade
} image quality. Is there a good way to avoid this?

Dear Chris,
I have had excellent luck with old photos by re-photographing
them. The lighting has to be arranged so that there are no spurious
reflections, but this is not too difficult. Using a digital camera will
make scanning the photos unnecessary, and using film will give you
a high-resolution negative. You will probably want to add a scale
bar to the original to account for changes in magnification. You
also need to take care that both the center and the edges of the print
are in focus, since they can be at significantly different distances
from the lens. Good luck.
Yours,
Bill Tivol




From daemon Mon Aug 14 09:17:07 2000



From: marian miller :      millermn-at-email.uc.edu
Date: Mon, 14 Aug 2000 10:02:53 -0400
Subject: coiled bodies, transcriptosomes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


is there a detailed and comprehensive reference (with em pictures) to
coiled bodies, pol II transcriptosomes (and pol I and pol III (?)),
interchromatin granules, perichromatin fibrils, peripheral condensed
chromatin, and of perinucleolar chromatin, fibillar centers, dense
fibrillar component, granular component .....and other that someone
knows of?????i would be eternally grateful for a lead to such a
paper(s) Thanks you, marian miller




From daemon Mon Aug 14 10:03:58 2000



From: Chris Baker :      chris-at-collegewafer.com
Date: Mon, 14 Aug 2000 10:48:36 -0800
Subject: Si MEMS, GaAs, InP, GaN, PtSi, SOI & More

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Visit http://www.collegewafer.com for your free quote, or call (800) 713-9375
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From daemon Mon Aug 14 10:40:01 2000



From: Karen Pawlowski :      karen.pawlowski-at-worldnet.att.net
Date: Mon, 14 Aug 2000 10:24:39 -0500
Subject: subscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


subscribe




From daemon Mon Aug 14 11:45:10 2000



From: Tamara Howard :      howard-at-cshl.org
Date: Mon, 14 Aug 2000 12:24:19 -0400 (EDT)
Subject: Re: coiled bodies, transcriptosomes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The only EM-type ref I know of is from 1990; Raska et al. in Electron
Microsc. Rec., 3:301-353. I can make a photocopy of my photocopy if you
are interested, but the images are mush. it is called :
"Immunocytochemistry of the Cell Nucleus".

The other refs I know of are mostly text and LM/FM, not TEM. You may have
to just lookup the individual authors who work on these stuctures...I
don't know if there are any sectioned images of transcriptosomes, but the
others are all out there. See papers from the labs of David Spector,
Robert Ochs, Marc Thiry, Daneholt, Aebi....There is a review from 1993 by
Spector that talks about the regions and has refs to EM papers:
"Macromolecular Domains within the Cell Nucleus" Annu. Rev. Cell Biol.,
1993 9:265-315.

Hope these help! If anyone sends you the ref for a good EM review, could
you please post it to the server?

Tamara Howard
CSHL (IGC-land)

On Mon, 14 Aug 2000, marian
miller wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} is there a detailed and comprehensive reference (with em pictures) to
} coiled bodies, pol II transcriptosomes (and pol I and pol III (?)),
} interchromatin granules, perichromatin fibrils, peripheral condensed
} chromatin, and of perinucleolar chromatin, fibillar centers, dense
} fibrillar component, granular component .....and other that someone
} knows of?????i would be eternally grateful for a lead to such a
} paper(s) Thanks you, marian miller
}
}
}




From daemon Mon Aug 14 14:42:27 2000



From: W. K. Beagley :      beagley-at-alma.edu
Date: Mon, 14 Aug 2000 07:29:08 -0400
Subject: scanning prints on lustre paper

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


With just one picture, you can usually retouch in Photoshop to restore
the image. If you have many, then it is probably worth re-photographing
them in flat light and through a polarizing filter. This should remove
the reflections, but it can also will increase contrast, causing loss of
detail in the very light and very dark areas of the image



From daemon Mon Aug 14 15:25:03 2000



From: Cynthia J. Zeissler :      cynthia.zeissler-at-nist.gov
Date: Mon, 14 Aug 2000 16:11:52 -0400
Subject: prep: large samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am posting this question for a colleague who is having trouble with the
large size of his samples.

"I am trying to find a way to fix and embed calcified carotid
endarterectomy specimens such that I will be able to obtain serial cross
sections. The problem that I am encountering thus far is that I am unable
to embed the 5cm specimens in a large paraffin block to allow sectioning
because such
large blocks either

a) do not fit on standard microtomes and therefore must be cut with
sliding microtomes resulting in more distortion of the specimen

b) fall off of the blocks after 1 or 2 sections are obtained because
the force of the blade against the mounted specimen shears the embedded
specimen from the cassette or resin embedding block.

How can I fix and embed large specimens (~5cm long) such that I can obtain
serial cross sections of the entire specimen for reconstructions without
having to chop the specimen into small labeled pieces first and embed each
of these pieces
individually?"

Thank you
********
Cynthia J. Zeissler
Physical Scientist
National Institute of Standards and Technology
cynthia.zeissler-at-nist.gov
Tel. 301-975-3910
FAX. 301-417-1321



From daemon Mon Aug 14 18:46:41 2000



From: Robert Mixon :      mixonr-at-ohsu.edu
Date: Mon, 14 Aug 2000 16:33:09 -0700
Subject: RE: Prep large samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Cynthia: We routinely cut 3.5 to 4.0 cm paraffin blocks and cut some that exceed 5 cm in length and width on a rotary microtome. The limitation is the length of the up and down travel on your microtome. You can use a vise -type holder to hold these blocks. You can always have an adapter made if you can't purchase one that will hold large paraffin blocks. You might not want to use cassettes for this purpose but simply make a large stiff paper mold in the shape of a cube to hold the specimen like all the old original paraffin specimens were done. As far as cutting calcified blocks this may be impossible in paraffin under some circumstances if the blocks have not been decalcified first...depends on the amount of calcium. Serial sections will be a real challenge on specimens of this size and character and do not permit ready starting and stopping once commenced.




From daemon Tue Aug 15 02:44:42 2000



From: Steve Chapman :      PROTRAIN-at-CompuServe.COM
Date: Tue, 15 Aug 2000 03:29:32 -0400
Subject: ISO9000 requirements

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Gary

I know this may be a bit delayed (holidays you know) but maybe I can add a
few points.

The micron marker is powered by the same electronics that produce
magnification i.e. an error in magnification equals an error in micron
marker too!

I do not know how old your instrument is or what make but this does make a
considerable difference in the accuracy of magnification. It is "normal"
to hope to get within 10% of the readout with an X to Y difference of less
than 5%. If the following rules are not followed errors of up to 25% are
quite easily obtained.

1. The sample must be a fairly flat surface

2. The sample must not be tilted

3. Turn OFF all electronic magnification and focus devices - dynamic
focus, scan rotation, tilt correction

Use a 2160 lines/mm metal or TEM standard (a carbon grating replica, much
cheaper and they work very well [see Spi]) to calibrate the machine but be
aware that the spot size setting will have an influence upon the
magnification accuracy!

The most accurate method for sample to sample consistency is to use the
focus control as little as possible and use the Z control to obtain focus.
In older instruments there was no zoom magnification linked to focus, so
change the focus and you change the magnification. On modern instruments
zoom magnification linked to focus is used in an attempt to hold the
magnification correct.


After many years of testing instruments of all types, with clients on our
courses there is no doubt that modern instruments are very good in relation
to magnification accuracy, but some of the older machines were real dogs!

Good luck

Steve Chapman

Senior Consultant Protrain
Tel & Fax - 44 (0)1280 814774
E-mail- protrain-at-emcourses.com
Web Site - www.emcourses.com
Protrain for SEM, TEM & EDX Training World Wide with our own full time
Professional staff
Courses in Australia, Canada, Europe, New Zealand, South Africa, Taiwan,
UK and USA



From daemon Tue Aug 15 06:54:56 2000



From: Alan Bright :      bright-at-dial.pipex.com
Date: Tue, 15 Aug 2000 12:20:40 +0100
Subject: prep: large samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Cynthia,

Would you please forward this to your colleague:-

You did not state the thickness of sections you are try to cut, this
information could be important.

} From what you state, the only thing you have not tried is using a cryostat,
this would give you a stable specimen without being as hard as using resin,
our cryostats are routinely sectioning undecalcified bone, the 5cms. size
would not pose a problem either.

Best Regards

Alan Bright

Bright Instrument Co.Ltd.
St Margaret's Way
Huntingdon
PE18 6EB
England

Tel No:+44 (0)1480 454528
Fax No:+44 (0)1480 456031
Email: AlanBright-at-brightinstruments.com
Web Site: www.brightinstruments.com



-----Original Message-----
} From: Cynthia J. Zeissler [mailto:cynthia.zeissler-at-nist.gov]
Sent: 14 August 2000 21:12
To: Microscopy-at-sparc5.microscopy.com


I am posting this question for a colleague who is having trouble with the
large size of his samples.

"I am trying to find a way to fix and embed calcified carotid
endarterectomy specimens such that I will be able to obtain serial cross
sections. The problem that I am encountering thus far is that I am unable
to embed the 5cm specimens in a large paraffin block to allow sectioning
because such
large blocks either

a) do not fit on standard microtomes and therefore must be cut with
sliding microtomes resulting in more distortion of the specimen

b) fall off of the blocks after 1 or 2 sections are obtained because
the force of the blade against the mounted specimen shears the embedded
specimen from the cassette or resin embedding block.

How can I fix and embed large specimens (~5cm long) such that I can obtain
serial cross sections of the entire specimen for reconstructions without
having to chop the specimen into small labeled pieces first and embed each
of these pieces
individually?"

Thank you
********
Cynthia J. Zeissler
Physical Scientist
National Institute of Standards and Technology
cynthia.zeissler-at-nist.gov
Tel. 301-975-3910
FAX. 301-417-1321





From daemon Tue Aug 15 07:45:20 2000



From: Wright, Cheryl W :      CWright-at-Sikorsky.com
Date: Tue, 15 Aug 2000 07:34:19 -0500
Subject: Zip Shot Program

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi everyone,

Has anyone used the program called "Zip Shot"? I have a 1830 Amray SEM with
a Noran Sigma EDS System. I would like to be able to print out my results
using this program. I am not familiar with Zip Shot, it has been
recommended to me. Thanks!

Cheryl Wright
Laboratory Operations
Sikorsky Aircraft
(203) 386-5746 (cwright-at-Sikorsky.com)





From daemon Tue Aug 15 09:05:57 2000



From: Won Sik Kim :      kimwonsi-at-pilot.msu.edu
Date: Tue, 15 Aug 2000 09:50:15 -0400 (EDT)
Subject: AISI 4340 steel in TEM (fwd)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


}
} We have a AISI4340 steel sample. This sample contains iron, carbon and
} nickel. We ion milled the sample. When we put the sample into our TEM,
} we cannot align or focus the microscope. we are operating a JEOL100CX
} microscope.
} Any suggestions for imaging this sample?
}
Thank you
Won-Sik Kim

kimwonsi-at-msu.edu




From daemon Tue Aug 15 09:17:22 2000



From: adriana-at-cena.usp.br
Date: Tue, 15 Aug 2000 10:43:16 -0300
Subject: website of SBMM (thanks to all for info)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear colleagues:

thanks to everyone who sent me information about SBMM (Brazilian Society of
Microscopy and Microanalysis)

Their current website and e-mail are:

http://www.dema.ufscar.br/sbmm/

sbmm-at-power.ufscar.br.

The website of the SBMM - Regional Chapter Rio de Janeiro is:

http://venus.rdc.puc-rio.br/sbme2/sbmeport.htm (currently under construction)

Adriana

Adriana Pinheiro Martinelli Rodriguez, PhD
Laboratorio de Biotecnologia Vegetal
CENA, Universidade de Sao Paulo
Av. Centenário 303, Cx. Postal 96
13400-970, Piracicaba, SP, Brasil
phone: +55-19- 429-4694
fax: +55-19- 429-4610
adriana-at-cena.usp.br
http://www.cena.usp.br/labs/labbiotecveg.htm







From daemon Tue Aug 15 10:07:32 2000



From: White, Woody N :      Woody.N.White-at-mcdermott.com
Date: Tue, 15 Aug 2000 10:48:00 -0500
Subject: Re:Zip Shot Program

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Cheryl,

Have not heard of ZipShot. Have used HyperSnap for windows as a screen
capture.
HS seems to be flexible and works nicely.
OTOH...
I have forgotten if the Sigma is running W95/8... If so have you tried
ALT-PrintScreen? This will capture the displayed image to the clipboard
(at the current monitor resolution). You can then paste into an image
editor like Photoshop...

Woody White
Mcdermott Technology Inc.



The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Hi everyone,

Has anyone used the program called "Zip Shot"? I have a 1830 Amray SEM with
a Noran Sigma EDS System. I would like to be able to print out my results
using this program. I am not familiar with Zip Shot, it has been
recommended to me. Thanks!

Cheryl Wright
Laboratory Operations
Sikorsky Aircraft
(203) 386-5746 (cwright-at-Sikorsky.com)



From daemon Tue Aug 15 12:45:20 2000



From: Vetrano, John S :      john.vetrano-at-pnl.gov
Date: Tue, 15 Aug 2000 10:31:00 -0700
Subject: AISI 4340 steel in TEM (fwd)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Since this material is magnetic you will need to reduce the amount of material
that you put in the microscope. The first cut is to just make the 3 mm disk
thinner at the start, perhaps 100 µm or less. At our lab we also use undersized
(1mm or 2.3 mm) specimens and occasionally embed them in another material to
make up the 3mm before we thin them. Probably the best way is to use the
"window" polishing technique (see Hirsch et al.) which minimizes the amount of
material while maximizing thin area. However I find it difficult to do
correctly.

If you contact me offline I can put you in touch with the person at our lab who
does the most of this type of work. By the way, we also order our microscopes
with "extra strength" objective stigmators for working with magnetic materials.

Cheers, John Vetrano
john.vetrano-at-pnl.gov

----------
} From: Won Sik Kim
Sent: Tuesday, August 15, 2000 6:50 AM
To: microscopy-at-sparc5.microscopy.com


}
} We have a AISI4340 steel sample. This sample contains iron, carbon and
} nickel. We ion milled the sample. When we put the sample into our TEM,
} we cannot align or focus the microscope. we are operating a JEOL100CX
} microscope.
} Any suggestions for imaging this sample?
}
Thank you
Won-Sik Kim

kimwonsi-at-msu.edu






From daemon Tue Aug 15 14:24:42 2000



From: Garrison, Becky :      becky.garrison-at-jax.ufl.edu
Date: Tue, 15 Aug 2000 15:11:08 -0400
Subject: LM Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does anyone have a light microscope for sale appropriate for a first year
medical student?
Student lives in Jacksonville FL
Will attend med school in Los Angeles.
Please respond off line:

Becky Garrison
Pathology Supervisor
ph 904-549-6237
fx 904-549-4290



From daemon Tue Aug 15 15:43:26 2000



From: Tom Phillips :      PhillipsT-at-missouri.edu
Date: Tue, 15 Aug 2000 15:28:24 -0500
Subject: Nordic antibody company????

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am trying to find a web or e-mail address for "Nordic" an antibody
supplier in Tilburg Netherlands. My web searches haven't worked.
Anyone out there deal with this supplier of immunocytochemical
antibodies, etc. Thanks, tom

--
Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)



From daemon Tue Aug 15 15:53:56 2000



From: jfb :      jfb-at-uidaho.edu
Date: Tue, 15 Aug 2000 13:42:13 -0700
Subject: Re: AISI 4340 steel in TEM (fwd)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Won-Sik Kim,

It sounds as if your sample may have become magnetized slightly. That
is exactly what happened with my JEOL 1200EX-2 when I tried to examine
iron particles which were slightly magnetic. I took the specimen grid
and placed it into the coil I use to demagnetize my forceps with, and
that solved the problem.

Franklin Bailey
University of Idaho
Electron Microscopy Center
Moscow, ID 83844-2204
jfb-at-uidaho.edu




From daemon Tue Aug 15 19:34:27 2000



From: Thomas, Larry (PNNL) :      Larry.Thomas-at-pnl.gov
Date: Tue, 15 Aug 2000 17:17:27 -0700
Subject: RE: AISI 4340 steel in TEM (fwd)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Reducing the size of your magnetic sample as John Vetrano has suggested is the
best start. Here are some other things to consider and to try with the JEOL
100CX. (This question was last discussed on the listserver a year or so ago as
I recall).

1. Be sure the sample is firmly held in the side-entry sample holder so it
doesn't get pulled out by the magnetic field of the objective lens in the
microscope. Sample holders with a spring-clip clamping mechanism are especially
prone to this unpleasantness.

2. If you are using a double-tilt holder, check the mechanism for looseness to
avoid having the sample spontaneously tilt to a position where the holder
cannot be withdrawn from the microscope without scraping the polepiece or
aperture holder.

3. Insert and withdraw the sample holder with the microscope in the
low-magnification mode (objective lens off) to help avoid problems #1 and 2.

4. Pre-align the microscope in darkfield mode with a normal (nonmagnetic)
sample before inserting the magnetic one. The available range of beam tilt
correction in the 100CX microscope is much larger in the darkfield image control
mode than it is in brightfield. A strongly magnetic sample will affect the beam
tilts and image stigmation, but not the image focus.

5. Correct the alignments as follows: (a) Focus the image with the mechanical
Z-axis translation rather than with the objective lens focus. (b) Align the
beam tilts by voltage centering the image (darkfield mode to get enough tilting
range). (c) Stigmate the image near its center --preferably with an unused
stigmator channel so the normal one is not messed up for other users.

6. Repeat the alignment (#5) each time you tilt the sample or translate it a
long way. The alignment corrections will increase a lot when the sample is
tilted beyond ~5 degrees off horizontal, but should be manageable at most tilts
unless the sample is too thick.

Larry Thomas
larry.thomas-at-pnl.gov



From: Vetrano, John S
Sent: Tuesday, August 15, 2000 10:31 AM
To: 'Microscopy Listserver'
Cc: 'kimwonsi-at-msu.edu'
Subject: FW: AISI 4340 steel in TEM (fwd)

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America
To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
-----------------------------------------------------------------------.


Since this material is magnetic you will need to reduce the amount of
material
that you put in the microscope. The first cut is to just make the 3 mm
disk
thinner at the start, perhaps 100 µm or less. At our lab we also use
undersized
(1mm or 2.3 mm) specimens and occasionally embed them in another
material to
make up the 3mm before we thin them. Probably the best way is to use
the
"window" polishing technique (see Hirsch et al.) which minimizes the
amount of
material while maximizing thin area. However I find it difficult to do
correctly.

If you contact me offline I can put you in touch with the person at our
lab who
does the most of this type of work. By the way, we also order our
microscopes
with "extra strength" objective stigmators for working with magnetic
materials.

Cheers, John Vetrano
john.vetrano-at-pnl.gov

----------
} From: Won Sik Kim
Sent: Tuesday, August 15, 2000 6:50 AM
To: microscopy-at-sparc5.microscopy.com
Subject: AISI 4340 steel in TEM (fwd)

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America
To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
-----------------------------------------------------------------------.


}
} We have a AISI4340 steel sample. This sample contains iron, carbon
and
} nickel. We ion milled the sample. When we put the sample into our TEM,
} we cannot align or focus the microscope. we are operating a
JEOL100CX
} microscope.
} Any suggestions for imaging this sample?
}
Thank you
Won-Sik Kim

kimwonsi-at-msu.edu








From daemon Wed Aug 16 01:37:53 2000



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Wed, 16 Aug 2000 18:23:14 GMT+1200
Subject: Regeneration of molecular seive

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi

Can someone advise me the appropriate temperature for regeneration of
molecular seive material used in a foreline trap in a diff
pump/rotary pump system on an SEM?

tia

rtch


Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand



From daemon Wed Aug 16 09:02:00 2000



From: Fred.Shaapur-at-SEMATECH.Org
Date: Wed, 16 Aug 2000 08:48:25 -0500
Subject: Job Opening: FA Engineer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} The following announcement is posted for:
}
} Roger Reyes, Group Leader
} Failure Analysis Lab
} International SEMATECH
} 2706 Montopolis Drive
} Austin, TX 78741
} (512) 356-3842
} roger.reyes-at-sematech.org
}
} To apply, please contact Roger directly.
} **********************************************
} JOB TITLE: FA Engineer
}
} JOB SUMMARY: To provide critical support to the Advanced Tool Development
} Facility (ATDF) and International SEMATECH's projects.
}
} JOB RESPONSIBILITIES:
} * Continuously improve quality and accuracy of lab analysis and general
} lab cycle time.
} * Engineer would work on jobs that are beyond the technicians level,
} providing in-depth analysis and advice on complex problems.
} * Engineer would be working closely with technicians so that learning and
} development occurs.
} * Engineer would attend technical staff meetings.
} * Some report writing and technical presentations.
} * Engineer will keep pace with the leading edge technology.
}
} QUALIFICATIONS:
} * Engineering degree.
} * Experience in the semiconductor industry is preferred.
} * Knowledge with databases.
} * Knowledge on wet chemistries to delineate CMOS layers.
} * Knowledge of Failure Analysis tool set. ( such as SEM, FIB,CVD, RIE
} etc...)
} * Good people skills.
}
}
}
}



From daemon Wed Aug 16 10:49:36 2000



From: Rahbari, Ramin :      RAMIN.RAHBARI-at-pfizer.com
Date: Wed, 16 Aug 2000 10:25:44 -0400
Subject: Nordic antibody company????

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Nordic Immunological Laboratories, Drawer 2517 , Capistrano Beach, CA,
92624-0517, Tele: 714.498.4467, Toll Free: 800.554.6655, FAX: 714.498.0138,

Nordic Immunological Laboratories NV, De Merodelei 24/5 P.O. Box 62,
Turnhout, , B-2300, Tele: 014.42.62.80, Toll Free: , FAX: ,

Nordic Immunological Labs Ltd., Dairy House Moneyrow Green, Holyport,
Maidenhead, Berks, , Tele: 0628.24978, Toll Free: , FAX: 0628.24978,

Serono Nordic AB, P.O. Box 103 , Sollentuna, , S-191 22, Tele: 08.613.52.00,
Toll Free: , FAX: 08.623.52.10,

Nordic Labs, Drawer 2517 , Capistrano Beach, CA, 92624-0512, Tele: , Toll
Free: , FAX: ,

Nordic Bio - Nordic Biosite AB, Propellervägen 6A , TÄBY, , S-183 62 , Tele:
46 (0)8 630 02 32, Toll Free: , FAX: 46 (0)8 756 94 90, www.biosite.se

Nordic Biosite AB, Propellervägen 6A , TÄBY, , S-183 62 , Tele: 46 (0)8 630
02 32, Toll Free: , FAX: 46 (0)8 756 94 90, www.biosite.se

Nordic Immunological Laboratories BV, Langestraat 55-61 P.O. Box 22,
Tilburg, , NL-5000 AA, Tele: 013.42.11.70, Toll Free: , FAX: 013.13364589,

Nordic Immunological Laboratories BV, Langestraat 55-61 P.O. Box 22,
Tilburg, , NL-5000 AA, Tele: 013.42.11.70, Toll Free: , FAX: 013.13364589



Ramin Rahbari
Pfizer Global Research & Development
Drug Safety Evaluation
2800 Plymouth Road
Ann Arbor, MI 48105
Voice (734) 622-3383
Fax (734) 622-5001
Ramin.Rahbari-at-Pfizer.com


-----Original Message-----
} From: Tom Phillips [mailto:PhillipsT-at-missouri.edu]
Sent: Tuesday, August 15, 2000 4:28 PM
To: Microscopy-at-sparc5.microscopy.com


I am trying to find a web or e-mail address for "Nordic" an antibody
supplier in Tilburg Netherlands. My web searches haven't worked.
Anyone out there deal with this supplier of immunocytochemical
antibodies, etc. Thanks, tom

--
Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)



From daemon Wed Aug 16 12:03:36 2000



From: Chris Jeffree :      cjeffree-at-srv0.bio.ed.ac.uk
Date: Wed, 16 Aug 2000 17:45:55 +0100
Subject: JEOL 6700

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear all

I would be grateful for feedback offline from any users of the
new(ish) JEOL 6700 FESEM, which appears still to be rather rare
in UK.

Many thanks
Chris
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Dr Chris Jeffree
University of Edinburgh
Biological Sciences EM Facility
Daniel Rutherford Building
King's Buildings EDINBURGH EH9 3JH
Tel: +44 (0) 131 650 5345
FAX: +44 (0) 131 650 6563

Inveresk Cottage, 26 Carberry Road,
Inveresk, Musselburgh, Midlothian EH21 8PR, UK
Tel: +44 (0) 131 665 6062 / Mobile 07710 585 401
FAX: +44 (0) 131 653 6248
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~



From daemon Wed Aug 16 18:05:28 2000



From: Theresa Fassel :      tfassel-at-scripps.edu
Date: Wed, 16 Aug 2000 15:52:10 -0500
Subject: TEM-source of gelatin for cryo immunogold

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello
I am looking for a good source for gelatin for cryo-immunogold experiments.
In the many papers on the subject I cannot find where the authors ordered
the gelatin or any purity information. Any information would be appreciated.
Thank you
Theresa
Theresa A. Fassel
Core EM Unit, Mail Box MB-32
The Scripps Research Institute
10,550 N. Torrey Pines Rd.
LaJolla, CA 92037-1027
tel: (858)-784-8182
fax: (858)-784-8193
tfassel-at-scripps.edu



From daemon Wed Aug 16 18:36:43 2000



From: Mary C. Pfauth :      mpfauth-at-teleport.com
Date: Wed, 16 Aug 2000 16:28:09 -0700 (PDT)
Subject: Zeiss eyepiece needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am looking for one each Zeiss KPl-W 10X eyepiece, catalog no. 46 40 42.
Have just checked eBay with no success. does anyone have one for sale or
can anyone suggest a source? Thanks, Mary Pfauth

John P.B. & Mary
mpfauth-at-teleport.com




From daemon Wed Aug 16 20:40:55 2000



From: Kalman Rubinson :      kr4-at-is2.nyu.edu
Date: Wed, 16 Aug 2000 21:21:39 -0400 (EDT)
Subject: Re: Zeiss eyepiece needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On Wed, 16 Aug 2000, Mary C. Pfauth wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I am looking for one each Zeiss KPl-W 10X eyepiece, catalog no. 46 40 42.
} Have just checked eBay with no success. does anyone have one for sale or
} can anyone suggest a source? Thanks, Mary Pfauth


I may have a spare in the lab. I'll check tomorrow.

Kal






From daemon Thu Aug 17 06:52:45 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Thu, 17 Aug 2000 06:40:25 -0500
Subject: Re: Ask-A-Microscopist: Buying Optical Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Janet

Please look at the Project Micro Pages on How to buy microscopes.
There is alot of valuable advice there.

http://www.msa.microscopy.com/ProjectMicro


I will also be forwarding your Email to Caroline Schooley (schooley-at-mcn.org)
as well as the Microscopy Listserver. Caroline
will have the absolute latest information from the National Microscopy
meeting which a large fraction of us from the US Microscopy Community
are at this week. The Listserver members will be able to advise you
on the rest of your questions, I have never used this microscope so
cannot give you good advise on it's purchase.

Nestor


}
} Email: jfallos-at-hotmail.com
} Name: Janet Jacobson-Fallos
} School: Bliss Discovery School
}
} Question: Hello!
}
} First, I need to tell you that I'm the TEACHER! :)
} I haven't done anything with a microscope since college Biology 101. I am
} hoping you might be able -- and willing -- to help me...
}
} I have been looking for quite some time to purchase a simple, yet high
} quality microscope for use by highly-motivated middle school-aged
} students. To find something of investment quality -- yet on a teacher's
} out-of-pocket budget, I decided to look for a used Zeiss, Leica, Nikon or
} Olympus. I had hoped to obtain a microscope with a 10x eyepiece and 4
} objectives -- incl. a 100x oil immersion. I've had a difficult time
} finding anything under $250.00
}
} I now have the opportunity to purchase an Olympus Model K ; circa 1970's?;
} w/ a 10x eyepiece and 10x, 40x and 100x objectives; X,Y slide mechanism,
} course and fine adjustments with locks. It has no illuminator, so I would
} need to purchase one separately. The price is $140. I have seen used
} illumination kits for anywhere from $40. -$200. I don't know if the 100x
} objective on the microscope is designed for oil-immersion or not. (I am
} currently awaiting the seller's answer to that question...) The microscope
} doesn't come with any accesories -- no dust cover, case, slides, manuals,
} books, etc. :(
}
} If you are familiar with Olympus microscopes, and hopefully the K model in
} particular, I would appreciate any information/comments you might provide -
}
} ---------------------------------------------------------------------------






From daemon Thu Aug 17 08:00:51 2000



From: Roberto Garcia :      rgarcia-at-unity.ncsu.edu
Date: Thu, 17 Aug 2000 08:47:19 -0400
Subject: Tensile Test Holder for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I was wondering if anyone could provide some information on tensile test
fixtures for SEM's. Particularly if you have one and if you like or dislike
it. I also need some manufacturers. The holder will be for a Hitachi 3200N
microscope. Thanks.
______________________________________________
Roberto Garcia
Senior Analyst, Metallography
NC State University / Analytical Instrumentation Facility
Campus Box 7531 Room 318 EGRC
1010 Main Campus Dr.
Raleigh, NC 27695-7531
(919) 515-8628
(919) 515-6965 Fax
rgarcia-at-unity.ncsu.edu
http://spm.aif.ncsu.edu/aif
http://spm.aif.ncsu.edu/asm/asm_home.htm
____________________________________________




From daemon Thu Aug 17 11:10:44 2000



From: Karen Kelley :      klv-at-biotech.ufl.edu
Date: Wed, 16 Aug 2000 23:58:44 -0400
Subject: Looking for reference

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am looking for a copy of: Lewis J, Nelson J, Elder H: Radioimmunoassay of
an antibiotic: gentamicin. Nature [New Biol] 239:1128, 1969
If anyone has this old paper sitting around please respond by email.

Thank you
Karen Kelley
Senior Electron Microscopist
UF Biotechnology Electron Microscopy Core Lab
Box 118525 Gainesville Florida 32611
lab:352-392-1184 fax: 352-846-0251
email: klv-at-biotech.ufl.edu
http://www.biotech.ufl.edu/~emcl/staff/karenpage.html



From daemon Thu Aug 17 12:57:13 2000



From: Michael Reiner :      Elektronenmikroskopie-at-web.de
Date: Thu, 17 Aug 2000 19:43:05 +0200
Subject: Re: Arteries fixation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Valerie,
per se your protocol looks pretty good. I have three suggestions. Fix it like that, try again perfusion fixation because it would preserve endothelium at best. Maybe you can even omit the Glut.
Second, try tannic acid, this enhances contrast while preserving antigenicity.
Third, try either LR-White resin or LR-Gold. Don´t cure it in the oven, use the accelerator in the cold (-20°C).
I am dealing with immunoelectronmicroscopy on endothelia for over two and a half years now and we got now the breakthrough in finding a balance between structure preservation and antigenicity.

If you would like to discuss this in more detail, don´t hesitate to contact me.

michael.reiner-at-uni-koeln.de
Michael Reiner
University of Cologne, Germany
Dept. of Anatomy I

} Valerie wrote:
} Dear all,
} In my laboratory, we study vascular endothelium with electron microscopy
} (Guinea pig). We tried perfusion to fix tissue but endothelium was removed.
} We fix them with 4% paraformaldehyde + 0,1 % Gluta + 0,1 % picric ac in
} phosphate buffer pH 7,4 overnight. Artery are then embedded on unicryl for
} immunocytochemistry. Tissue was stain with uranyl acetate and Reynolds.
} With this procedure cell structure isnot very evident, membrane is well
} defined but not very contrasted. Ac locate on all the section, not very
} locate in a particular place of cells.
} We tried other first and second antibodies but it was the same thing.
} What can we do?
} Do you have any suggestion?
} Thanks
}
}

Michael Reiner
University of Cologne, Germany
Dept. of Anatomy I
_______________________________________________________________________
1.000.000 DM gewinnen - kostenlos tippen - http://millionenklick.web.de
IhrName-at-web.de, 8MB Speicher, Verschluesselung - http://freemail.web.de




From daemon Thu Aug 17 22:01:16 2000



From: Pbgrover-at-aol.com
Date: Thu, 17 Aug 2000 22:34:09 EDT
Subject: roughing pump tipped over

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


A well-meaning, non-microscopist friend to whom I have rented out a room in
my lab decided to spiffy up the place while I was out of town. He tipped the
roughing pump to a JEOL 35 up on end and stood it in the corner. When I got
back from my trip, there was, of course, oil all over the floor.

The microscope was not in service at the time, but I want to place it in
service as a back-up SEM some time in the future. My question:

What do I need to do (other than replace the lost oil) before starting up the
pump?

Paul Grover



From daemon Thu Aug 17 23:10:21 2000



From: MELISSA ANN LEWIS :      lewisma-at-medicine.ufl.edu
Date: Thu, 17 Aug 2000 22:51:42 -0500
Subject: gelatin for cryo-immunogold

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In our lab we use a 275 bloom gelatin from fisher. Catalog number
G8-500. We use a 2% solution. We went through many gelatins and finally
was recommended to use the 275 bloom. Good luck:)





From daemon Fri Aug 18 03:20:34 2000



From: Simon Dumbill :      simon.dumbill-at-aeat.co.uk
Date: Fri, 18 Aug 2000 09:03:42 +0100
Subject: Re: tensile test fixtures for SEMs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Roberto,
We have a tensile stage with 500 degC heating capability which was built for us by Oxford Instruments (that division may now be part of Gatan?). The stage is for a LEICA S440 though I believe they could adapt to it for other microscopes. The system is good and has worked well.
Good luck,
Simon

{} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {}
Dr Simon Dumbill
Team Leader, Microstructural Characterisation
AEA Technology Nuclear Science
220, Harwell
Didcot
Oxon OX11 0RA
UK

Tel: +44 (0)1235 434245
Fax: +44 (0)1235 435941
Email: Simon.Dumbill-at-aeat.co.uk

} } } "Roberto Garcia" {rgarcia-at-unity.ncsu.edu} 17 August 2000 13:47:19 } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


I was wondering if anyone could provide some information on tensile test
fixtures for SEM's. Particularly if you have one and if you like or dislike
it. I also need some manufacturers. The holder will be for a Hitachi 3200N
microscope. Thanks.
______________________________________________
Roberto Garcia
Senior Analyst, Metallography
NC State University / Analytical Instrumentation Facility
Campus Box 7531 Room 318 EGRC
1010 Main Campus Dr.
Raleigh, NC 27695-7531
(919) 515-8628
(919) 515-6965 Fax
rgarcia-at-unity.ncsu.edu
http://spm.aif.ncsu.edu/aif
http://spm.aif.ncsu.edu/asm/asm_home.htm
____________________________________________





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Registered in England and Wales, number 3095862.



From daemon Fri Aug 18 03:51:37 2000



From: Rosemary White :      Rosemary.White-at-pi.csiro.au
Date: Fri, 18 Aug 2000 18:37:58 +1000
Subject: postdoc position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




POST DOCTORAL FELLOW - ROOT/RHIZOSPHERE BIOLOGIST
$46K - $52K PLUS SUPERANNUATION

CSIRO PLANT INDUSTRY

Canberra Australia



We are seeking a motivated Postdoctoral Fellow to join a team of
researchers in a project with the overall aim of developing a conceptual
framework for understanding the role of root/rhizosphere biology in crop
productivity. Specifically you would be investigating the impacts of
different crop management practices (e.g. conservation cropping) on root
architecture and structure, and on the in situ localisation and composition
of rhizosphere microbes. The work will involve the use of modern methods
of microscopy and bacterial identification and will also involve
collaboration with other soil biologists, agronomists and farmers.


The successful applicant will have a PhD and experience with root and soil
biology, preferably with field-grown plants, and technical expertise and/or
interest in the practical application of modern microscopy and
microbiological methods to the research project.

This position is for a term of 3 years, with possible extension to 5 years.

This post-doctoral fellowship is sponsored by the Grains Research
Development Corporation as a component of a major new initiative in Soil
Biology Research. The position is within the CSIRO Division of Plant
Industry. The Division has a staff of 700 with 60% located at its
headquarters in Canberra and other groups located at seven sites across
Australia.

For further information contact Professor Margaret McCully (email mccully
-at-pi.csiro.au ; phone 61 2 6246 5343) or Dr John Kirkegaard (email
j.kirkegaard-at-pi.csiro.au ; phone 61 2 6246 5080) An Information Package
and Selection Documentation can be obtained by contacting the answering
service on 61 2 6246 5434 or fax 61 2 6246 5000 or email
recruit-at-pi.csiro.au This information is also available via our web site
http://www.pi.csiro.au/

Applicants must obtain and respond to the Selection Criteria and Duty
Statement. Address your application for the above position quoting
Reference No. PG:00082 and include details of your experience, skills and
qualifications together with the names of 3 references. Please mark
'Confidential' and forward to: Trish Borger, Human Resources, CSIRO Plant
Industry, GPO Box 1600, Canberra ACT 2601 by Sepy. 15/ 2000 (notional).







From daemon Fri Aug 18 05:01:52 2000



From: David Vowles :      djv23-at-msm.cam.ac.uk
Date: Fri, 18 Aug 2000 10:47:35 +0100 (BST)
Subject: Re: Tensile Test Holder for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Roberto,

Deben Research manufacture an excellent range of micro-testing equipment
for SEM chamber operation. Their details are :

Deben Research
11-15 High St
Stowmarket
Suffolk IP14 6QL UK
Tel: +44 1728 861320
Fax: +44 1728 861350
Email: info-at-deben.co.uk
WWW: http://www.deben.co.uk

Cheers,

David Vowles
Electron Microscopy Unit
Dept of Materials Science and Metallurgy
University of Cambridge
Pembroke St Cambridge
UK CB2 3QZ
Tel: +44 1223 334325
Fax: +44 1223 334567
Email: djv23-at-msm.cam.ac.uk

On Thu, 17 Aug 2000, Roberto Garcia wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I was wondering if anyone could provide some information on tensile test
} fixtures for SEM's. Particularly if you have one and if you like or dislike
} it. I also need some manufacturers. The holder will be for a Hitachi 3200N
} microscope. Thanks.
} ______________________________________________
} Roberto Garcia
} Senior Analyst, Metallography
} NC State University / Analytical Instrumentation Facility
} Campus Box 7531 Room 318 EGRC
} 1010 Main Campus Dr.
} Raleigh, NC 27695-7531
} (919) 515-8628
} (919) 515-6965 Fax
} rgarcia-at-unity.ncsu.edu
} http://spm.aif.ncsu.edu/aif
} http://spm.aif.ncsu.edu/asm/asm_home.htm
} ____________________________________________
}
}
}




From daemon Fri Aug 18 10:06:35 2000



From: Su, Phy-Huynh :      psu-at-shctampa.usf.edu
Date: Fri, 18 Aug 2000 10:53:16 -0400
Subject: New microscopes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear MSA subscribers,

My research department is putting in a grant to ask money to buy a new set
of microscopes which should work well with both bright and dark field, and
coupled with color digital camera, and image analyzer. So far, we have got
a quote from ZEISS for around $77K.
My director wants to have the best, easy to use, easy to upgrade with great
service for years to come.

Some has told us that ZEISS has the best lens quality in the world, and
others said NIKON lenses are compatible to ZEISS, and its cameras are
superior. Again others would recommend LEICA because it also makes
excellent instruments for less money, with great service in Tampa Bay area.


Please help with your experience and knowledge in this field. Any satisfied
customers? We don't have to buy the whole set from one company. We can get
microscopes from one, digital camera from others.

We work most with Bone and Cartilage, paraffin, frozen, tissue culture, and
plastic, if this information will have any weight on choosing which company
we should go for.

Thank you very much for all your feedback!





From daemon Fri Aug 18 12:19:47 2000



From: Rogerio Lucio de Almeida :      ralmeida-at-cedro.fisica.ufmg.br
Date: Fri, 18 Aug 2000 14:57:29 -0300 (EST)
Subject: Re: postdoc position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




******************************************
* Rogerio Lucio de Almeida *
* Molecular Beam Epitaxy Laboratory *
* Phys. Department UFMG-Brazil *
* ralmeida-at-fisica.ufmg.br *
* Phone 31 499-5622 fax +55 31 499-5600 *
******************************************



On Fri, 18 Aug 2000, Rosemary White wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
}
} POST DOCTORAL FELLOW - ROOT/RHIZOSPHERE BIOLOGIST
} $46K - $52K PLUS SUPERANNUATION
}
} CSIRO PLANT INDUSTRY
}
} Canberra Australia
}
}
}
} We are seeking a motivated Postdoctoral Fellow to join a team of
} researchers in a project with the overall aim of developing a conceptual
} framework for understanding the role of root/rhizosphere biology in crop
} productivity. Specifically you would be investigating the impacts of
} different crop management practices (e.g. conservation cropping) on root
} architecture and structure, and on the in situ localisation and composition
} of rhizosphere microbes. The work will involve the use of modern methods
} of microscopy and bacterial identification and will also involve
} collaboration with other soil biologists, agronomists and farmers.
}
}
} The successful applicant will have a PhD and experience with root and soil
} biology, preferably with field-grown plants, and technical expertise and/or
} interest in the practical application of modern microscopy and
} microbiological methods to the research project.
}
} This position is for a term of 3 years, with possible extension to 5 years.
}
} This post-doctoral fellowship is sponsored by the Grains Research
} Development Corporation as a component of a major new initiative in Soil
} Biology Research. The position is within the CSIRO Division of Plant
} Industry. The Division has a staff of 700 with 60% located at its
} headquarters in Canberra and other groups located at seven sites across
} Australia.
}
} For further information contact Professor Margaret McCully (email mccully
} -at-pi.csiro.au ; phone 61 2 6246 5343) or Dr John Kirkegaard (email
} j.kirkegaard-at-pi.csiro.au ; phone 61 2 6246 5080) An Information Package
} and Selection Documentation can be obtained by contacting the answering
} service on 61 2 6246 5434 or fax 61 2 6246 5000 or email
} recruit-at-pi.csiro.au This information is also available via our web site
} http://www.pi.csiro.au/
}
} Applicants must obtain and respond to the Selection Criteria and Duty
} Statement. Address your application for the above position quoting
} Reference No. PG:00082 and include details of your experience, skills and
} qualifications together with the names of 3 references. Please mark
} 'Confidential' and forward to: Trish Borger, Human Resources, CSIRO Plant
} Industry, GPO Box 1600, Canberra ACT 2601 by Sepy. 15/ 2000 (notional).
}
}
}
}
}




From daemon Fri Aug 18 12:39:32 2000



From: DrJohnRuss-at-aol.com
Date: Fri, 18 Aug 2000 13:27:48 EDT
Subject: topographic measurement paper from Phila. meeting

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Several people requested copies of the full paper I presented at the
Philadelphia meeting on the characterization of surface topography using AFM
and SEM. I have uploaded a 720 kB "pdf" (acrobat) file containing a summary
of my verbal presentation with images of the slides, for anyone interested.
You can download it at {http://personal.rdu.bellsouth.net/~jcr5/MSA_paper.pdf}

John C. Russ



From daemon Fri Aug 18 15:46:32 2000



From: Tom Phillips :      PhillipsT-at-missouri.edu
Date: Fri, 18 Aug 2000 15:30:43 -0500
Subject: Re: New microscopes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I own a Zeiss, a Nikon and 2 Olympus scopes. Which is best? Depends
on the application. Brand X may have the greatest optics but if the
price prevents you from buying all the types of objectives needed for
different approaches, then you will end up making do with the "wrong"
objective and lose any real gain. The 3 manufacturers are all very
similar. There are subtle advantages for each type (e.g., Nikon has
the best working distance objectives). First, decide what features
on objectives you need (e.g., water immersion, high NA, working
distance, high UV thruput) and then buy the one you can afford.

Service is a function of your local rep and the company has very
little to do with it in my experience. Zeiss owns their reps these
days but it is still the local guys who you deal with.

The digital camera can come from any vendor - the best ones are
probably not made my the microscope companies.


That's my prejudiced two cents worth!


} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

--
Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)



From daemon Fri Aug 18 19:43:02 2000



From: Ken Converse :      qualityimages-at-netrax.net
Date: Fri, 18 Aug 2000 20:20:34 -0700
Subject: Re: roughing pump tipped over

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Pbgrover-at-aol.com-at-sparc5.microscopy.com wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} A well-meaning, non-microscopist friend to whom I have rented out a room in
} my lab decided to spiffy up the place while I was out of town. He tipped the
} roughing pump to a JEOL 35 up on end and stood it in the corner. When I got
} back from my trip, there was, of course, oil all over the floor.
}
} The microscope was not in service at the time, but I want to place it in
} service as a back-up SEM some time in the future. My question:
}
} What do I need to do (other than replace the lost oil) before starting up the
} pump?
}
} Paul Grover
Paul,
After you finish cleaning up the oil, turn the pump by hand a couple of
turns just to be sure all is OK. It should be fine.

Ken Converse
owner
Quality Images
third party SEM service
Delta, PA



From daemon Fri Aug 18 20:28:37 2000



From: Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 18 Aug 2000 18:13:35 -0700
Subject: Re: New microscopes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Good points. I am using Zeiss Axioskops despite near zero help
from Zeiss. I used Olympus for years. But the newer BX series
did not produce the images I need. Olympus was/is user friendly.
Zeiss is user hostile as I see it. But their equipment is very good.

It takes a while to get the systems to work when there is little
or no help from the maker. This is a result of their cancellation
of dealers. Some Zeiss items can be found at great prices from
abandoned Zeiss dealers.

gary g.


At 01:30 PM 8/18/00, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From daemon Sat Aug 19 00:51:03 2000



From: Lola Norton :      lola-at-utep.edu
Date: Fri, 18 Aug 2000 23:30:39 -0600
Subject: Re: roughing pump tipped over

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


You might also want to check your exhaust manifold and change the filter -
just in case it was contaminated by some of the oil.

--
Lola Norton
Research Engineer
ESEM Facility
EDU-114
(915) 747-8049

University of Texas at El Paso
Chemistry Department Mail Code:00513
El Paso, TX 79968

} From: Ken Converse {qualityimages-at-netrax.net}
} Organization: Quality Images
} Reply-To: qualityimages-at-netrax.net
} Date: Fri, 18 Aug 2000 20:20:34 -0700
} To: "Pbgrover-at-aol.com"-at-sparc5.microscopy.com
} Cc: Microscopy-at-sparc5.microscopy.com
} Subject: Re: roughing pump tipped over
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Pbgrover-at-aol.com-at-sparc5.microscopy.com wrote:
} }
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} } A well-meaning, non-microscopist friend to whom I have rented out a room in
} } my lab decided to spiffy up the place while I was out of town. He tipped the
} } roughing pump to a JEOL 35 up on end and stood it in the corner. When I got
} } back from my trip, there was, of course, oil all over the floor.
} }
} } The microscope was not in service at the time, but I want to place it in
} } service as a back-up SEM some time in the future. My question:
} }
} } What do I need to do (other than replace the lost oil) before starting up the
} } pump?
} }
} } Paul Grover
} Paul,
} After you finish cleaning up the oil, turn the pump by hand a couple of
} turns just to be sure all is OK. It should be fine.
}
} Ken Converse
} owner
} Quality Images
} third party SEM service
} Delta, PA
}
}
}




From daemon Sat Aug 19 04:52:01 2000



From: Lucille A. Giannuzzi :      lag-at-mail.ucf.edu
Date: Sat, 19 Aug 2000 17:30:08 -0400
Subject: 1 day TEM short course

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Several folks have made good points on this, so I am throwing in my two
cents as well. There are many considerations for defining a "good"
microscope. Personal preference (ergonmetrics, habits), price, and most
importantly the task/procedures it is used for. You dont use a student
microscope for R&D or vice versa.

When I consider a microscope purchase, I look only at the universals....we
are scientists and use them accordingly, even in production settings. If I
was a teacher, I would chose differently. My personal choice over the last 7
years is to go with the Oly BMAX line. I never cared for Oly until they got
their act back together with the introduction of that line. It is a
modification of Zeiss lineage. Zeiss still has the best objectives, but it
is moot. Most of that difference you cannot resolve with your eyes.....just
arent capable of doing so. So, I choose Oly objectives or used Zeiss
objectives.

One last note. The russian scopes are incredibly enticing at their price,
and they are pretty good in quality. My only problem with them is they are
still built like their tanks.

Lou Solebello
----- Original Message -----
} From: Gary Gaugler {gary-at-gaugler.com}
To: Tom Phillips {PhillipsT-at-missouri.edu}
Cc: MSA listserver {Microscopy-at-sparc5.microscopy.com}
Sent: Friday, August 18, 2000 6:13 PM


A one day TEM short course will be offered through the American Vacuum
Society in Boston on Oct. 2, 2000.

For details please see: www.vacuum.org

*******************************************************************
Lucille A. Giannuzzi, Ph.D.

Associate Professor, Mechanical, Materials, and Aerospace Engineering
Director, UCF/Cirent Materials Characterization Facility
University of Central Florida
12443 Research Parkway, Suite 305
Orlando, FL 32826 email lag-at-mail.ucf.edu phone (407) 275-4354,5,6
fax (407) 275-4321
--------------------------------------------------------------------
"Good judgement comes from experience.

Experience comes from making bad judgement."

Mark Twain
********************************************************************





From daemon Sat Aug 19 16:51:16 2000



From: Gary Ward :      gward-at-zoo.uvm.edu
Date: Sat, 19 Aug 2000 17:40:24 -0600
Subject: Montage FR2 film recorder

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have inherited a Montage FR2 film recorder which looks to be in great
shape except that I have been unable to figure out how to drive it and
technical support for this product appears to be non-existent. Does anyone
know how to interface the FR2 with a Mac, to generate slides from
PowerPoint or Canvas?

Thanks

Gary Ward

Department of Microbiology and Molecular Genetics
214 Stafford Hall
University of Vermont
Burlington VT 05405 USA

email gward-at-zoo.uvm.edu









From daemon Sun Aug 20 17:36:54 2000



From: richard black :      m02jmy00-at-cwcom.net
Date: Sun, 20 Aug 2000 23:09:51 +0100
Subject: Re: New microscopes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have looked at microscopes from most of the main suppliers and I have
yet to find one that compares to the Leica range for quality (both
optical and build) and, as far as I am concerned, ease of use and
ergonomics. I use at the moment an Olympus AX which is excellent
optically but is a nightamare to use. Nothing is easy to get to and
every time I move my hand from the focussing or lens changer, I catch
the stage even after two years of using the thing. The Zeiss microscope
I had on demo was not much better. The Nikons seem quite good ( I have
three of these in use)but I have not had a research one with really good
optics but might go with one of them under certain circumstances i.e.
financial constraints. The Leica DMR which I use for image analysis is
a pleasure to use in every way and the optics are unbeatable.
Richard Black





From daemon Mon Aug 21 08:29:01 2000



From: Markus Drechsler :      Markus.Drechsler-at-rz.uni-jena.de
Date: Mon, 21 Aug 2000 15:18:11 +0200
Subject: Re: Montage FR2 film recorder

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Gary,

the only adress I know in the USA is

Presentation Technologies
Martin Wieczorkowski
779 Palomar Avenue
Sunnyvale, Ca 94086,USA

It is 6 years ago that I tried to contact the company, so I don't know if
it exists any more.
I purchased the Montage in 1993 from a German Company

Raebel EDV
Kolbermoorer Str. 8a
D-83043 Bad Aibling
Tel.: ++49-806-4015 or 4016
Fax: ++49-8061-35714
email:info-at-raebel.de
WWW: http://www.raebel.de

According to their homepage they sell the Montage FR2 up to now.

Cheers,
Markus




At 17:40 19/08/00 -0600, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


*******************************************
Dr. Markus Drechsler
Friedrich-Schiller-Universitaet Jena
Institut fuer Pharmazie
Lehrstuhl fuer Pharmazeutische Technologie
Lessingstr.8
D-07743 Jena

Tel: +49 (0) 36 41 / 9-49903 Buero
+49 (0) 36 41 / 9-49914 Labor
+49 (0) 36 41 / 9-49915 TEM
e-mail:Markus.Drechsler-at-uni-jena.de
http://www.uni-jena.de/~b7drma

Sekretariat
Tel: +49 (0) 36 41 / 9-49900
Fax: +49 (0) 36 41 / 9-49912

*******************************************



From daemon Mon Aug 21 08:56:01 2000



From: Philip Oshel :      peoshel-at-facstaff.wisc.edu
Date: Mon, 21 Aug 2000 08:52:21 -0500
Subject: Re: scanning prints on lustre paper

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Chris,

Two thoughts:
1) Photograph the images with very fine grain film like Tech Pan or
better. You'll lose a little from the copying, but probably less than
with digitizing. The negatives can then be digitized and also
archived.
2) See if someone at UE has a drum scanner -- a university print
shop, or some local pre-press business. Perhaps even the Art
department, if they do computer art (or not).

Phil

} I apologise if this is a little out-of-gamut for this list, but I have been
} trying to make digital copies of archival material which only exists
} as small prints on lustre paper. On a flat bed scanner, the textured
} surface causes objectionable specular reflections which degrade
} image quality. Is there a good way to avoid this?
}
} Chris
} ==========================================
} Dr. Chris Jeffree
} University of Edinburgh
} BIOSEM - Biological Sciences Electron Microscope Facility
} Institute of Cell and Molecular Biology
} Daniel Rutherford Building
} King's Buildings, Mayfield Road
} EDINBURGH, EH9 3JH, Scotland, UK
} Tel. #44 (0) 131 650 5345
} FAX. #44 (0) 131 650 6563
} Mobile 07710 585 401
} email c.jeffree-at-ed.ac.uk
} =========================================

--
}}}}}}}}}}}}}}}}}{{{{{{{{{{{{{{{{{
Philip Oshel
Supervisor, AMFSC and BBPIC
Dept. of Animal Health and Biomedical Sciences
University of Wisconsin
1656 Linden Drive
Madison, WI 53706-1581
voice: (608) 263-4162
fax: (608) 262-7420 (dept. fax)



From daemon Mon Aug 21 10:50:05 2000



From: Leona Cohen-Gould :      lcgould-at-mail.med.cornell.edu
Date: Mon, 21 Aug 2000 11:40:06 -0400
Subject: Re: M&M Meeting in Phila

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All,
Forgive me for sounding like a cheerleader, but I want to thank the LAC
gang in Philadelphia for a job very well done! The meeting was a pleasure
to attend. I hope everyone is able to take a well desreved breather this
week.

Next year in Long Beach.

Lee

Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175





From daemon Mon Aug 21 11:16:08 2000



From: Stuart McKernan :      mckernan-at-cems.umn.edu
Date: Mon, 21 Aug 2000 11:01:57 -0500 (CDT)
Subject: Re: M&M Meeting in Phila

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Responding to the message of {v03100003b5c6b7d13bc9-at-[140.251.145.24]}
from Leona Cohen-Gould {lcgould-at-mail.med.cornell.edu} :
} Hi All,
} Forgive me for sounding like a cheerleader, but I want to thank the LAC
} gang in Philadelphia for a job very well done! The meeting was a pleasure
} to attend. I hope everyone is able to take a well desreved breather this
} week.
}
} Next year in Long Beach.
}

A sentiment shared by the whole program committee for the Microscopy and
Microanalysis 2000 meeting!

Plan on Long Beach for MandM 2001.



__________________
Stuart McKernan stuartm-at-tc.umn.edu
Office:(612) 626-7594
IT Characterization Facility, University of Minnesota Desk:(612) 624-6009
100 Union Street S. E., Minneapolis, MN 55455 NEW-} Fax:(612) 625-5368




From daemon Mon Aug 21 15:12:03 2000



From: Greg Erdos :      gwe-at-biotech.ufl.edu
Date: Mon, 21 Aug 2000 15:59:18 -0400
Subject: Fwd: Teaching

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html





} Although I do not do confocal microscopy, I have been asked to, describe
} it to new graduate students as part of a general lecture on microscopy. I
} would like to present a Z-series in color along with a reconstructed
} image. Can anyone point me to a source (preferably electronic) where I
} might find such a thing.
}
} TIA
}
} Greg Erdos

Gregory W. Erdos, Ph.D.
Assistant Director, Biotechnology Program
PO Box 110580
University of Florida
Gainesville, FL 32611

Ph. 352-392-1295 Fax: 352-846-0251



From daemon Mon Aug 21 15:14:56 2000



From: PESTOEM-at-aol.com
Date: Mon, 21 Aug 2000 16:06:02 EDT
Subject: Philips EM 200

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello All, we have a client with an old Philips EM200. The client had a large
number of Mercury Batteries stored in his Refrigerator and now the supply is
used-up.
Does anyone have an alternate or a supplier ? The last 2 Batteries exploded.
Thank you. Peter A. Stolzenberg, PESTO Inc., Philadelphia , PA
215-699-6160 FAX 215-699-5275




From daemon Mon Aug 21 17:15:12 2000



From: Awbrey, Donald :      DonaldAwbrey-at-hmhs.com
Date: Mon, 21 Aug 2000 17:52:45 -0500
Subject: TEM questions about filter syringes.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Microscopy listserv readers:
Hildy has asked that I relay this message to the microscopy listserv,
because she no longer has access to the account through which she
subscribed.
--John
=================


} From: Hildegard H Crowley
{hcrowley-at-du.edu}





}
} Dear EM Netters:
}
} I'm interested in purchasing sterile syringe filters to be used
} to rinse my grids. I have found some that has a pore size
} of 0.1 microns. They have a typical fluid retention of {= 50
} lambda. The membrane is made of hydrophilic polyethersulfone.
} Has anyone used these or the filter membrane? If so, do
} they require priming with preboiled deionized water?
}
}


Donald G. Awbrey, HT(ASCP) QIHC
Electron Microscopy / Image Analysis
Donald Awbrey-at-hmhs.com
donaldawbrey-at-hotmail.com
(817)-878-5647




From daemon Mon Aug 21 18:49:24 2000



From: Ekstrom, Harry :      harry.ekstrom-at-honeywell.com
Date: Mon, 21 Aug 2000 16:37:00 -0700
Subject: SEM Position Available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



SEM Position Available

Honeywell in Phoenix, Arizona is looking for an experienced SEM/EDS/WDS
operator to assist in the analysis of aerospace materials. Experience also
desired in metallography of aerospace materials. For more information
contact Juan Trillo at 602-231-2321 or reply to juan.trillo-at-honeywell.com



Harry Ekstrom
Materials Laboratory
(602) 231-2744
e-mail: harry.ekstrom-at-honeywell.com




From daemon Tue Aug 22 01:45:13 2000



From: Erasmus, Willem (WJ) :      willem.erasmus-at-sasol.com
Date: Tue, 22 Aug 2000 08:30:36 +0200
Subject: Topographic measurement paper

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Microscopists

Has anybody tried to download the summary of the verbal presentation at the
Philadelphia meeting on the characterization of surface topography using AFM

and SEM By Dr John Russ ? For some reason all I get is a blank page. I'm not
sure if it has something to do with my internet connection.

Regards
W. Erasmus

Willem Erasmus
Snr. Scientist, Basic Catalysis Research
Sasol Technology
Tel : +27 +16 9603954
Fax : +27 +16 9602826
E-mail : willem.erasmus-at-sasol.com




From daemon Tue Aug 22 04:25:43 2000



From: Dr Malcolm Roberts :      m.roberts-at-ru.ac.za
Date: Tue, 22 Aug 2000 11:13:17 +0200
Subject: Standards for Th and Pb

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear All,
We're in the process of setting up for U-Th-Pb chemical dating of
monazite, and are in need of standards for Th and Pb. A particularly
useful reference in this regard is that of Scherrer et al 2000 (Schweiz.
Mineral. Petrol. Mitt), which summarises all kinds of stuff. And I've a
couple of questions.
1) Does anyone know how to synthesise ThP2O7. Can it be done in the
same way as REE orthophosphates?
2) Does anyone have any ideas on the stability under the beam of
crocoite and it's suitability as a reference standard e.g., homogeneity,
zoning etc. etc.
Handy hints always helpful,
Malc.

--
Dr MP Roberts Phone: [+27](0)46 603 8313
Dept of Geology Fax: [+27](0)46 622 9715
Rhodes University Cell: 083 4060 262 (usually off)
6140 Grahamstown e-mail: m.roberts-at-ru.ac.za
SOUTH AFRICA





From daemon Tue Aug 22 07:07:02 2000



From: DrJohnRuss-at-aol.com
Date: Tue, 22 Aug 2000 07:55:40 EDT
Subject: Re: Topographic measurement paper

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



In a message dated 8/22/00 2:52:57 AM, willem.erasmus-at-sasol.com writes:

} Has anybody tried to download the summary of the verbal presentation at
} the Philadelphia meeting on the characterization of surface topography using
} AFM and SEM By Dr John Russ ? For some reason all I get is a blank page. I'm
} not sure if it has something to do with my internet connection.

The paper does seem to download OK. If you are having difficulty because your
browser doesn't like the ftp:// designation, try going to
{http://personal.rdu.bellsouth.net/~jcr5/index.html/} and selecting the paper
from there.



From daemon Tue Aug 22 07:13:10 2000



From: David_Bell-at-millipore.com
Date: Tue, 22 Aug 2000 08:04:03 -0400
Subject: Re: Topographic measurement paper

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hello,

I was able to successfully access the website referenced by Dr. Russ. I
did notice that immediately upon arriving at the site, it opened up Adobe
Acrobat. Perhaps your browser does not have that plug-in.

Hope this helps,



David A. Bell
Scientist
Electron Microscopy Lab
Millipore Corporation
80 Ashby Road
Bedford, MA 01730
(781) 533-2108




From daemon Tue Aug 22 07:26:44 2000



From: Rosemary Walsh :      rw9-at-psu.edu
Date: Tue, 22 Aug 2000 08:21:34 -0400
Subject: Re: Forwarded message from Hildy Crowley

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thanks Hildy! We will miss your thoughtful contributions to the list and
your wit!
Rosemary



From daemon Tue Aug 22 07:32:12 2000



From: Owen P. Mills :      opmills-at-mtu.edu
Date: Tue, 22 Aug 2000 08:22:53 -0400
Subject: Philips EM301 parts available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We are discarding a EM-301 within the next few days. There are many new
spare parts (electronic components, mechanical parts, etc.). Please contact
me directly using the email address below if you need anything.

Owen

=============================
Owen P. Mills
Electron Optics Facility Engineer
Michigan Technological University
Metallurgical & Materials Engineering
Rm 512 M&M Building
Houghton, MI 49931
906-487-2002
906-487-2934 (FAX)
opmills-at-mtu.edu




From daemon Tue Aug 22 07:32:36 2000



From: Joergen Bilde-Soerensen 5802 :      j.bilde-at-risoe.dk
Date: Tue, 22 Aug 2000 13:37:13 +0200
Subject: Re: Standards for Th and Pb

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Malcolm Roberts wrote:
} 2) Does anyone have any ideas on the stability under the beam of
} crocoite and it's suitability as a reference standard e.g., homogeneity,
} zoning etc. etc.

Dear Malcolm,

SPI uses crocoite and galena as Pb-standards in their minerals
standards for X-ray microanalysis. I take this to indicate that
crocoite is a suitable choice.

Best regards,
Jorgen Bilde-Sorensen

*****************************
J. B. Bilde-Soerensen
Senior Research Scientist, Ph. D.
Materials Research Department
Risoe National Laboratory
DK-4000 Roskilde
Denmark

e-mail: j.bilde-at-risoe.dk
phone: +45 46 77 58 02 (direct)
phone: +45 46 77 46 77 (switchboard)
fax: +45 46 77 57 58
website: http://www.risoe.dk/afm/Personal/jqbi/jqbi.htm



From daemon Tue Aug 22 07:52:26 2000



From: Chuck Butterick :      cbutte-at-ameripol.com
Date: 8/22/00 8:30 AM
Subject: Topographic measurement paper

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I successfully downloaded the pdf file but had to play with IP
address. Using

http://personal.rdu.bellsouth.net/~jcr5

I was able to get to a page where I used a right click on "summary"
and saved the file.

Thanks for the download, John.

Chuck Butterick
Engineered Carbons, Inc.



______________________________ Reply Separator _________________________________


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Dear Microscopists

Has anybody tried to download the summary of the verbal presentation at the
Philadelphia meeting on the characterization of surface topography using AFM

and SEM By Dr John Russ ? For some reason all I get is a blank page. I'm not
sure if it has something to do with my internet connection.

Regards
W. Erasmus

Willem Erasmus
Snr. Scientist, Basic Catalysis Research
Sasol Technology
Tel : +27 +16 9603954
Fax : +27 +16 9602826
E-mail : willem.erasmus-at-sasol.com







From daemon Tue Aug 22 09:46:59 2000



From: Facinelli, John :      john.facinelli-at-honeywell.com
Date: Tue, 22 Aug 2000 07:27:54 -0700
Subject: Microtome (used) wanted for purchase

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I am looking for a new or used microtome which will work well in sectioning
0.5-5 mil polymer films and also polymer pellets. A used manual microtome
would be fine. Also looking for a good bargain. Thanks!

John V. Facinelli Ph.D.
Senior Staff Scientist
Honeywell - Performance Polymers
* Tel: (804) 520-3519
* Fax: (804) 524-4099
* E-mail: John.Facinelli-at-honeywell.com




From daemon Tue Aug 22 11:24:58 2000



From: bradley-at-socrates.Berkeley.EDU
Date: Tue, 22 Aug 2000 09:09:28 -0700 (PDT)
Subject: a question about perfusion & gluteraldehyde

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Greetings all. I am a grad student in the psych dept at UC Berkeley. I am
attempting to do HRP histochemistry on rat brain tissue. I have
encountered several problems along the way. I hope someone on this list
will be gracious enough to either make a suggestion or point me towards a
resource which is helpful.

Briefly, my problems are these:

When I perfuse the animal via the left ventricle, frequently the lungs
fill up and the brain is poorly fixed, regardless of which
fixative I use. What's perplexing about this is that it seems to occur
only when the fixative is added; typically the blood flush goes perfectly
well. I usually don't move the 26 ga. needle when the solutions are
changed. Should I be putting the needle in the aorta, as opposed to the
L. ventricle? How can I do that without damaging the vessel?

I perfused a couple animals with 1% paraform/1.25% gluteraldehyde and
found the brains to be mushy-- virtually unsectionable. Despite the
problems with the lungs filling up & my worries about fixation, the
liver appeared to be hardened, so I assumed something had happened. The
glut was opened } 1 year ago, but I made the solution 48 hours
before the procedure. What might have caused the brain to be so
poorly fixed? Should I assume that the perfusion itself was poor, or, does
the age & contact with air matter when using gluteraldehyde?

Thanks. I hope this is the appropriate list for these kinds of
questions.


Sincerely,

bradley cooke
UC Berkeley




From daemon Tue Aug 22 12:04:13 2000



From: Maggy Piranian :      maggy-at-sparky2.esd.mun.ca
Date: Tue, 22 Aug 2000 14:24:01 -0200
Subject: spare xrd, microprobe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Probers, Diffractionists,
We have two old (antique?) instruments:

1. An approximately 25 year old Philips XRD that still worked when it was
set aside 10 years ago.

2. A JEOL JXA 50A (1976) microprobe that still works except for needing a
valve replaced. We have the rplacement valve.

These are free to someone who can pay for crating and shipping. Could you
circulate this message to appropriate individuals and institutions?

Thanks,

Maggy Piranian
Earth Sciences
Memorial University of Newfoundland
St. John's, Newfoundland
Canada
(709) 737 8244
maggy-at-sparky2.esd.mun.ca



From daemon Tue Aug 22 12:40:18 2000



From: Geoff McAuliffe :      mcauliff-at-UMDNJ.EDU
Date: Tue, 22 Aug 2000 13:29:36 -0400
Subject: Re: a question about perfusion & gluteraldehyde

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


"bradley-at-socrates.Berkeley.EDU"-at-sparc5.microscopy.com wrote:

} Greetings all. I am a grad student in the psych dept at UC Berkeley. I am
} attempting to do HRP histochemistry on rat brain tissue. I have
} encountered several problems along the way. I hope someone on this list
} will be gracious enough to either make a suggestion or point me towards a
} resource which is helpful.
}
} Briefly, my problems are these:
}
} When I perfuse the animal via the left ventricle, frequently the lungs
} fill up and the brain is poorly fixed, regardless of which
} fixative I use. What's perplexing about this is that it seems to occur
} only when the fixative is added; typically the blood flush goes perfectly
} well. I usually don't move the 26 ga. needle when the solutions are
} changed. Should I be putting the needle in the aorta, as opposed to the
} L. ventricle? How can I do that without damaging the vessel?
}
} I perfused a couple animals with 1% paraform/1.25% gluteraldehyde and
} found the brains to be mushy-- virtually unsectionable. Despite the
} problems with the lungs filling up & my worries about fixation, the
} liver appeared to be hardened, so I assumed something had happened. The
} glut was opened } 1 year ago, but I made the solution 48 hours
} before the procedure. What might have caused the brain to be so
} poorly fixed? Should I assume that the perfusion itself was poor, or, does
} the age & contact with air matter when using gluteraldehyde?
}
} Thanks. I hope this is the appropriate list for these kinds of
} questions.
}
} Sincerely,
}
} bradley cooke
} UC Berkeley

Bradley:

First, a 26 gauge needle is too small to deliver an adequate volume of
fixative unless you are perfusing an embryo or a very young animal.
However, the real problem is that you are going through the
interventricular septum with the tip of the needle and perfusing the pulmonary
circulation, not the systemic circulation. Thus firm lungs and mushy brain.
Get a small piece of cork sheet or thin tubing. Poke the perfusion needle
(20 or 18 ga.) through it so that only 2-3 mm protrudes. When entering the
left vent. use the cork as a guide to prevent penetrating too far AND stay a
bit to the right (your right, not the animal's) of the apex of the heart. The
angle of "attack" should be about like this backslash mark. \ Also, make
sure the cork does not slide on the shaft of the needle, otherwise your guide
will become useless.
Also, see if you can find an experienced perfuser to help you. Practice.

Geoff
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************





From daemon Tue Aug 22 13:25:53 2000



From: Leona Cohen-Gould :      lcgould-at-mail.med.cornell.edu
Date: Tue, 22 Aug 2000 14:14:12 -0400
Subject: Re: a question about perfusion & gluteraldehyde

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Bradley,
I haven't done whole animal perfusions in years, so I'll leave comments
about that to others, but will say something about your fix solution.
Your glutaraldehyde is OLD. It oxidizes over time and becomes pretty
worthless as a fixative. My advice is to buy a fresh supply, preferably in
small volumes. Many of the EM suppliers sell vials of 10 ml each of a
variety of concentrations, sealed under nitrogen or other inert gas, so
that you can open a fresh vial and have an easy dilution each time you need
to make fix.

You probably need to leave the brain immersed in fix for a period of time
after you've perfused it, but that will depend on how robust your antigen
is....how much fixation will it tolerate?

I'm sure if you talk to the people in UC Berkely's animal facility, someone
there should be able to help you get the perfusion set up properly. Its
their job to make sure that animals are handled within guidelines, and they
should be familiar with certain procedures.

Good luck, get fesh chemicals!
Lee

Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175





From daemon Tue Aug 22 13:33:40 2000



From: lcgould-at-mail.med.cornell.edu
Date: Tue, 22 Aug 2000 14:22:40 -0400
Subject: Re: a question about perfusion & gluteraldehyde

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hi Bradley,
I haven't done whole animal perfusions in years, so I'll leave comments
about that to others, but will say something about your fix solution.
Your glutaraldehyde is OLD. It oxidizes over time and becomes pretty
worthless as a fixative. My advice is to buy a fresh supply, preferably in
small volumes. Many of the EM suppliers sell vials of 10 ml each of a
variety of concentrations, sealed under nitrogen or other inert gas, so
that you can open a fresh vial and have an easy dilution each time you need
to make fix.

You probably need to leave the brain immersed in fix for a period of time
after you've perfused it, but that will depend on how robust your antigen
is....how much fixation will it tolerate?

I'm sure if you talk to the people in UC Berkely's animal facility, someone
there should be able to help you get the perfusion set up properly. Its
their job to make sure that animals are handled within guidelines, and they
should be familiar with certain procedures.

Good luck, get fesh chemicals!
Lee






From daemon Tue Aug 22 17:07:45 2000



From: Sandra Perkins :      skperkin-at-vt.edu
Date: Tue, 22 Aug 2000 18:08:12 -0500
Subject: Re: a question about perfusion & gluteraldehyde

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Bradley-

I agree with Geoff's suggestions. We actually use a 16 gauge gavage needle
and clamp it with a hemostat. This gives us good perfusion of the nervous
system. I'd like to suggest a great paper that may help you:

Andrew Fix and Robert Garman, Toxicologic Pathology, vol.28, no.1, pp.
122-131, 2000. "Practical Aspects of Neuropathology: A Technical Guide
for Working with the Nervous System."


Good luck,
Sandy Perkins



Laboratory for Neurotoxicity Studies
VA-MD Regional College of Vet Med
Virginia Tech





From daemon Tue Aug 22 18:35:32 2000



From: Glen MacDonald :      glenmac-at-u.washington.edu
Date: Tue, 22 Aug 2000 16:22:04 +0100
Subject: Re: a question about perfusion & gluteraldehyde

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Once you find a useful gauge of needle, use an emery stone to reduce the
bevel of the tip, or get a short stub adapter of that guage and give it
a slight bevel. This will prevent accidentally slicing the heart, or
your finger.

A permanent method to create an insertion stop is to mix dental acrylic
and build up a bead on the needle while rotating it. Continue rotating
until it hardens.

Glen
--

Glen MacDonald
Virginia Merrill Bloedel Hearing Research Center
Box 357923
University of Washington
Seattle, WA 98195-7923
glenmac-at-u.washington.edu
(206) 616-4156
(206) 616-1828 fax
************************************************************************
C:} The box said "Requires Windows95 or better". So I bought a Macintosh.
************************************************************************



From daemon Tue Aug 22 19:31:06 2000



From: Sara Miller :      saram-at-duke.edu
Date: Tue, 22 Aug 2000 20:18:02 -0400 (EDT)
Subject: Confocal Technical Position Open

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Experienced Confocal Microscopy/Electron Microscopy Technologist to work
in Research Laboratory of Department of Pathology


The person we seek will be responsible for organization of a new facility
that includes a confocal microscope and an electron microscope. The
position includes overall management of the microscopy facility, user
training, and user supervision. Requirements for the position include
experience with light and transmission electron microscopy. This
individual will oversee all aspects of specimen accession and processing,
operation of the microscopes, photography, record keeping, and
supervision of a technician. Knowledge of EM, biology, and pathology, as
well as photographic procedures is required. The individual will work
with only minimum supervision.

Position Requirements

Experience with confocal and digital imaging techniques, microinjection,
visualization of living cells containing fluorescent probes,
photobleaching, and fluorescence in situ hybridization.

Minimum of five years experience working in an electron microscopy
laboratory with hands-on experience in tissue processing, dark room
photography, and operation and maintenance of electron microscope.

Two years experience working on electron microscopy of human tissue
specimens with knowledge of histology and pathology.

Excellent interpersonal and organizational skills are essential.

BachelorUs degree in science desirable.

Desirable Experience

Expertise in training in the operation of confocal microscope systems is
a distinct advantage.

Familiarity with light microscopy methods, immunofluorescent staining,
use of fluorescent probes for physiologic measurements and the general
principles of cell biological research are critical. Significant facility
with computers is desired.

Responsibilities

Serve as the technical manager of the facility and be responsible for the
operation and maintenance of the confocal and EM microscope facility.

Perform routine transmission EM, including tissue processing,
ultramicrotomy, and examination; do preventative maintenance on the
equipment; maintain the lab, order supplies, schedule instruments, and
oversee billing.

Image analysis at the light, confocal and electron microscopic levels and
preparation of micrographs for publication.


Send resume to:
Suzanne Mirra, M.D., Chair
Department of Pathology
Box 25
SUNY-Brooklyn
450 Clarkson Av.
Brooklyn, NY 11203-2098

-----------------
Submitted by
Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265




From daemon Tue Aug 22 19:35:51 2000



From: Sara Miller :      saram-at-duke.edu
Date: Tue, 22 Aug 2000 20:21:22 -0400 (EDT)
Subject: Histotechnologist Position Open

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Experienced Histotechnologist to work in Neuropathology Research
Laboratory of Department of Pathology

The person we seek will be responsible for maintaining a neuropathology
research laboratory in the Department of Pathology at SUNY Downstate
Medical Center. Knowledge of immunohistochemistry, special stains, and
histopathology is required. Previous experience in handling central
nervous system tissue preferred. The individual will work with only
minimum supervision.

Position Requirements

Minimum of five years experience working in a laboratory with hands-on
experience in tissue processing, histochemistry, immunohistochemistry,
and operation of a light microscope. Experience with technical aspects of
neuropathology and performing special stains including silver
(Bielschowsky) and myelin stains.

Two years experience working on immunohistochemistry of CNS tissue
specimens with knowledge of immunoreagents and experimental protocols.

BachelorUs degree in science desirable.

Laboratory skills including communications, ability to comply with safety
and laboratory regulations, maintenance of laboratory equipment and
resources, operation of computers and office equipment.

Advanced computer skills (word processing and database management) essential.

Desirable Experience

Confocal microscopy desirable.

Salary commensurate with experience


Responsibilities

Purchasing supplies and equipment, budget reports, laboratory maintenance
and brain banking.

Will operate all microscopic, photographic and computer equipment, and
keep accurate records of all laboratory experiments and procedures. Light
and fluorescent microscopy; tissue processing for paraffin embedding,
sectioning and slide stainer for immunohistochemical procedures; computer
imaging (PhotoShop); general photography; and library and web searches

Familiarity with computer software for keeping records, report
preparation and table/figure construction using Microsoft Office software
(Word, Excel, PowerPoint) as well as Endnote and Adobe PhotoShop.

Send resume to:

Suzanne Mirra, M.D., Chair
Department of Pathology
Box 25
SUNY-Brooklyn
450 Clarkson Av.
Brooklyn, NY 11203-2098

-------------------
Submitted by
Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265




From daemon Tue Aug 22 19:38:37 2000



From: Sara Miller :      saram-at-duke.edu
Date: Tue, 22 Aug 2000 20:24:34 -0400 (EDT)
Subject: EM Tech Position Open

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Experienced Electron Microscopy Technologist to work in Research
Laboratory of Department of Pathology


The person we seek will be responsible for the overall operation of the
EM laboratory in the Department of Pathology at SUNY Downstate Medical
Center. This individual will oversee all aspects of specimen accession
and processing, operation of the microscope, photography, record keeping,
and supervision of a technician. Knowledge of EM, biology, and pathology,
as well as photographic procedures is required. The individual will work
with only minimum supervision.

Position Requirements

Minimum of five years experience working in an electron microscopy
laboratory with hands-on experience in tissue processing, dark room
photography, and operation and maintenance of electron microscope.

Two years experience working on electron microscopy of human tissue
specimens with knowledge of histology and pathology.

BachelorUs degree in science desirable.

Laboratory management skills including effective written/verbal
communication skills to interact with a diverse group, ability to comply
with safety and laboratory regulations, maintenance of laboratory
equipment and resources, and operation of computers and office equipment.

Desirable Experience

Previous experience in confocal microscopy highly desirable.

Previous experience in performing immunocytochemical staining and
advanced computer skills usage (e.g. image analysis) is also desirable.

Salary commensurate with experience

Responsibilities

Maintain electron microscope in operating condition. Process clinical and
research tissues for "thick" and "thin" sectioning. Darkroom management
of photographic printing.


Send resume to:
Suzanne Mirra, M.D., Chair
Department of Pathology
Box 25
SUNY-Brooklyn
450 Clarkson Av.
Brooklyn, NY 11203-2098

-----------------
Submitted by:
Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265




From daemon Tue Aug 22 20:53:05 2000



From: Pitzenberger, Marcia H. :      marcia_pitzenberger-at-merck.com
Date: Tue, 22 Aug 2000 11:14:28 -0400
Subject: LM - need help with processing dog lens in plastic

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Listserver!

Does anyone have experience with processing lens from a dog eye in
plastic? The lens was fixed in 10% NBF. I'm trying glycol methacrylate,
using the JB-4 Plus kit. I trimmed the lens to 2-3 mm thick and infiltrated
overnight. I'm just sectioning the first block. Areas of the lens are
fractured. I'm thinking I need to try longer infiltration. But is there a
better way? Thanks for any advice.


Marcia Pitzenberger
Research Associate
Merck & Co.
WP45-251
West Point, PA 19438
(215) 652-9767 voice
marcia_pitzenberger-at-merck.com



From daemon Wed Aug 23 07:25:42 2000



From: jim :      jim-at-proscitech.com.au
Date: Wed, 23 Aug 2000 22:04:32 +1000
Subject: RE: a question about perfusion & gluteraldehyde

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Just to make the "GA is old" point a little clearer:
GA polymerises with time and higher temperatures; a nitrogen head does not
change much. Frozen, GA will last an awfully long time and if stored
refrigerated most people would still be happy with the product after six months
and more. While in the lab storage should not be a problem, we can be sure that
inappropriate storage is common.
The problem is in transit, when during the summer months the GA could see some
rather high temperatures during a truck or even an air journey. We have always
taken some care with GA, but being a long way from the manufacturer we have
adopted special precautions such as shipping with ice and mostly by air and
incoming shipments are also packed in ice and refrigerated whenever not in the
air. Its costly, but this assures a better product.
The high purity Formaldehyde 16% does not require refrigeration, keeps for
years and is an excellent fixative. I don't know how well this works in
perfusion fixation, but it may well be a good alternative.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Wednesday, August 23, 2000 4:23 AM,
"lcgould-at-mail.med.cornell.edu"-at-sparc5.microscopy.com
[SMTP:"lcgould-at-mail.med.cornell.edu"-at-sparc5.microscopy.com] wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} Hi Bradley,
} I haven't done whole animal perfusions in years, so I'll leave comments
} about that to others, but will say something about your fix solution.
} Your glutaraldehyde is OLD. It oxidizes over time and becomes pretty
} worthless as a fixative. My advice is to buy a fresh supply, preferably in
} small volumes. Many of the EM suppliers sell vials of 10 ml each of a
} variety of concentrations, sealed under nitrogen or other inert gas, so
} that you can open a fresh vial and have an easy dilution each time you need
} to make fix.
}
} You probably need to leave the brain immersed in fix for a period of time
} after you've perfused it, but that will depend on how robust your antigen
} is....how much fixation will it tolerate?
}
} I'm sure if you talk to the people in UC Berkely's animal facility, someone
} there should be able to help you get the perfusion set up properly. Its
} their job to make sure that animals are handled within guidelines, and they
} should be familiar with certain procedures.
}
} Good luck, get fesh chemicals!
} Lee
}
}
}




From daemon Wed Aug 23 07:58:01 2000



From: Missy Josephson :      josepem-at-vetmed.auburn.edu
Date: Wed, 23 Aug 2000 07:47:35 -0500
Subject: Re: perfusion and fixation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Bradley,
Perhaps an easier solution to the danger of penetrating the wrong heart
chamber is to use an over-the-needle Teflon I.V. catheter. Your animal care
personnel can procure them for you, or if you have a hospital handy, their
supply warehouse will have them. You can use a 20-23 gauge catheter in a
rat. The sharp needle protrudes through the lumen of the catheter; you
introduce the needle, bearing the catheter, into the left ventricle, just
enough to penetrate the muscle wall, then slide the Teflon catheter part
over the needle, deeper into the heart. With practice, I have been able to
guide the catheter into the ascending aorta. The needle is then slid
backwards out of the catheter and set aside. The catheter has a female hub
end that will accept IV tubing, syringe hubs or injection caps, whatever
your preference. Since this is a terminal procedure, you can clean the
catheter and needle and reuse them.
Good luck!
Missy

Eleanor M. Josephson, D.V.M., Ph.D.
Assistant Professor
Department of Anatomy, Physiology and Pharmacology
College of Veterinary Medicine
111 Greene Hall
Auburn University, AL 36849-5518
Ph. (334) 844-5423
FAX (334) 844-4542
josepem-at-vetmed.auburn.edu




From daemon Wed Aug 23 08:58:23 2000



From: Greg Erdos :      gwe-at-biotech.ufl.edu
Date: Wed, 23 Aug 2000 09:45:44 -0400
Subject: Sign-up calendar

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


During the Facilities Management session at MM2000, we discussed online
sign-up for instruments. At that time I could not tell anyone the
specifics of the one we use.
`It is free-ware and can be found at this site:

http://www.webprog.com/appoint/freetest.html

you can see it running at this site:

http://scope.biotech.ufl.edu/index.html
Gregory W. Erdos, Ph.D.
Assistant Director, Biotechnology Program
PO Box 110580
University of Florida
Gainesville, FL 32611

Ph. 352-392-1295 Fax: 352-846-0251



From daemon Wed Aug 23 09:44:13 2000



From: Nigel Browning :      browning-at-uic.edu
Date: Wed, 23 Aug 2000 08:48:28 -0600
Subject: postdoctoral position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


POSTDOCTORAL FELLOW IN INTERFACE PHYSICS


DEPARTMENT OF PHYSICS, UNIVERSITY OF ILLINOIS AT CHICAGO



{smaller} A postdoctoral fellow position is available at the University
of Illinois at Chicago for the development and application of novel
STEM/TEM techniques to the analysis of metal-oxide interfaces and grain
boundaries in metals. Research in the Interface Physics Group focuses
on the use of atomic resolution imaging and analytical techniques in
electron microscopy, coupled with theoretical simulations, to determine
the structure-property relationships at internal interfaces on the
fundamental atomic scale. Other related research programs within the
Interface Physics Group include ceramics, ionic conductors, high-Tc
superconductors and semiconductor materials/devices, and it is expected
that the successful applicant will work closely with the existing group
members in these areas. =20


The experimental facilities to perform this research are comprehensive:
a JEOL 2010 Field-Emission STEM/TEM featuring a 1.4=C5 probe size, "drift
free" stage, high-angle annular dark-field detector (Z-contrast), Gatan
Imaging Filter, Noran EDS, Gatan liquid nitrogen cooling stage, and
Gatan heating stage; a VG HB601 Field-Emission dedicated STEM featuring
a 2.2=C5 probe size and EDS and EELS capabilities; a JEOL 3010
conventional TEM with digital imaging capabilities and EDS; a JEOL 6320
=46ield-Emission SEM with EDS; and a JEOL JXA733 microprobe. In addition
to the electron microscopes, specimen preparation facilities include a
Gatan Duo-mill, Fischione precision ion-mill, SouthBay plasma cleaner
and Leica Ultramicrotome. The Interface Physics Group has a Silicon
Graphics R10000 Power Indigo workstation with the Molecular
Simulations' Cerius 2 package incorporating the CASTEP pseudopotential
code. The physics department has additional workstations and access to
the UIC Convex Exemplar Supercomputer and the National Center for
Supercomputing Applications at UIUC. =20


Successful candidates will be recent Ph.D. graduates in physics,
metallurgy, or materials science with a sound background in the
relevant materials issues and an ambition to be part of a developing
program pushing at the frontiers of interface physics. Please send a
resume and publication list to Professor Nigel D. Browning at the
address below. Prior experience in STEM or TEM is essential. =20
However, consideration will be based on the candidates overall
potential for success in the field and applicants with prior experience
in related fields are encouraged to apply. Positions are for one year
initially, normally renewed for a second year with possibilities
existing for further years. Salary is commensurate with experience.=20
UIC is an equal opportunity employer.

=20


Nigel D. Browning, PhD

{italic} Associate Professor

{/italic} University of Illinois at Chicago

Department of Physics (M/C 273)

845 West Taylor Street

Chicago. IL 60607-7059. USA


Tel: 312-413-8164

=46ax: 312-996-9016

e-mail: Browning-at-uic.edu


http://interface.phy.uic.edu


{/smaller}





___________________________________________________________________________


Nigel D. Browning, PhD

{italic} Associate Professor {/italic}

University of Illinois at Chicago

Department of Physics (M/C 273)

845 West Taylor Street

Chicago. IL 60607-7059. USA


Tel: 312-413-8164

=46ax: 312-996-9016

e-mail: Browning-at-uic.edu


http://interface.phy.uic.edu


___________________________________________________________________________





From daemon Wed Aug 23 10:55:40 2000



From: David L. Spector :      spector-at-cshl.org
Date: Wed, 23 Aug 2000 11:41:26 -0400
Subject: Position Available/Microscopy Core Technician

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Microscopy Core Technician.

Cold Spring Harbor Laboratory is seeking an experienced and responsible
microscopy technician for the laboratories core microscopy facility.
The individual should have practical expertise in transmission electron
microscopy, confocal and widefield fluorescence microscopy, digital
imaging, and microinjection. The successful candidate will be involved
in designing and carrying out experimental protocols for users, training
individuals in the use of various microscopes, and aligning microscopes
and keeping the facility operating at an efficient and high level of
productivity. Interested individuals should send their resume,
including a description of their expertise and the names and addresses
of 3 references to: Dr. David L. Spector, Cold Spring Harbor Laboratory,
One Bungtown Road, Cold Spring Harbor, New York 11724, email:
spector-at-cshl.org





From daemon Wed Aug 23 11:50:12 2000



From: Rick Harris :      raharris-at-ucdavis.edu
Date: Wed, 23 Aug 2000 09:39:07 -0700
Subject: Re: Sign-up calendar

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 09:45 AM 8/23/00 -0400, Greg Erdos wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


I would like to second the opinion of Greg Erdos. We have been using the
same calendar program and have been quite happy with it. You can see it
running at our site:

http://katie.ucdavis.edu/

then click on Schedule Time.





Rick A. Harris, Director
Microscopy and Imaging Facility
Section of Molecular and Cellular Biology
1241 Life Sciences Addition
University of California
Davis, CA
530 752 2914
530 754 7536 fax
http://katie.ucdavis.edu
raharris-at-ucdavis.edu



From daemon Wed Aug 23 12:21:44 2000



From: Bob Wise :      wise-at-vaxa.cis.uwosh.edu
Date: Wed, 23 Aug 2000 12:09:52 -0500
Subject: Poor man's cold stage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I would like to try to visualize ice in frozen plant leaves in a Hitachi
2460 variable pressure SEM and do not have access to a cold stage. I have
heard that a block of aluminum or copper at LN temperature may make a
usable substitute. Has anyone had any experience with this technique? Any
and all tips would be appreciated.

TIA

Bob
Dr. Robert R. Wise
Associate Professor of Plant Physiology
Department of Biology
University of Wisconsin Oshkosh
Oshkosh, WI 54901
(920) 424-3404
wise-at-uwosh.edu
http://www.uwosh.edu/departments/biology/wise/wise.html



From daemon Wed Aug 23 15:28:37 2000



From: Beverly_E_Maleeff-at-sbphrd.com
Date: Wed, 23 Aug 2000 16:14:36 -0400
Subject: Digital cameras for TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Colleagues:

We're considering putting a digital camera on our JEOL 1200EX STEM. Three
possibles are from Gatan, AMT, and SIS. Does anyone care to comment (positively
or negatively) about retrofitting an older instrument with a camera system, and
recommend one system over the other?

Regards,
Bev Maleeff
SmithKline Beecham Pharmaceuticals





From daemon Wed Aug 23 16:38:52 2000



From: Walck, Scott D. :      walck-at-ppg.com
Date: Wed, 23 Aug 2000 17:28:53 -0400
Subject: Digital cameras for TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have seen the results of a Gatan Megaview on an older Philips 420 which
was rather impressive. I have an older AMT on a 2000FX that works quite
well. I saw the SIS system at M&M. I think that you will be pleased with
whatever system that you buy. You may still need to use film occasionly,
but the bulk of your work will use the camera.
-Scott


-----Original Message-----
} From: "Beverly_E_Maleeff-at-sbphrd.com"-at-sparc5.microscopy.com
[mailto:"Beverly_E_Maleeff-at-sbphrd.com"-at-sparc5.microscopy.com]
Sent: Wednesday, August 23, 2000 4:15 PM
To: Microscopy-at-sparc5.microscopy.com


Colleagues:

We're considering putting a digital camera on our JEOL 1200EX STEM. Three
possibles are from Gatan, AMT, and SIS. Does anyone care to comment
(positively
or negatively) about retrofitting an older instrument with a camera system,
and
recommend one system over the other?

Regards,
Bev Maleeff
SmithKline Beecham Pharmaceuticals





From daemon Wed Aug 23 16:52:27 2000



From: Debby Sherman :      sherman-at-btny.purdue.edu
Date: 23 Aug 2000 16:43:57 -0500
Subject: Re: Poor Man's coldstage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


I did see this done by an application engineer when he was demonstrating a VP SEM. We really wanted to look at hydrated tissue and the only way was to freeze a metal specimen mount in liquid nitrogen and then put the sample on it as quiackly as possible. Then put it in the scope and, with the pressure as high as possible, let the vacuum "freeze-dry" the sample. If you were just wanting a quick look at hydrated material, this works fairly well. If you really want to do reproducible work with the frozen plant material, you really need a true cold stage. We do have a cryo stage and have used it for similar projects as well as for a whole host of other samples. It really is a very valuable accessory to our SEM.

Debby

Debby Sherman, Manager Phone: 765-494-6666
Microscopy Center in Agriculture FAX: 765-494-5896
Dept. of Botany & Plant Pathology E-mail: sherman-at-btny.purdue.edu
Purdue University
1057 Whistler Building
West Lafayette, IN 47907-1057


On Wednesday, August 23, 2000, Bob Wise {wise-at-vaxa.cis.uwosh.edu} wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From daemon Wed Aug 23 18:10:38 2000



From: Sally Stowe :      stowe-at-rsbs.anu.edu.au
Date: Thu, 24 Aug 2000 09:33:53 +1000
Subject: Re: Poor man's cold stage

Contents Retrieved from Microscopy Listserver Archives
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by ultra5.microscopy.com (8.9.1b+Sun/8.9.1) id SAA18205
for dist-Microscopy; Wed, 23 Aug 2000 18:06:30 -0500 (CDT)
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Reply-To: {tgc-at-gatan.com}




-----Original Message-----
} From: tgc [mailto:tgc-at-gatan.com]
Sent: Wednesday, August 23, 2000 3:43 PM
To: microscopy-at-msa.microscopy.com


Hi Bob,
We use the frozen block technique with a Hitachi 2250N (earlier
model than yours, also fitted with Peltier and ln2-cooled stages) quite
often for quick observations. It works well, but you only have a limited
time (few minutes) before the ice sublimes, followed by 15-30 minutes of
good stable viewing of the freeze-drying sample - if you want to examine ice
distribution in situ with any accuracy at all, I dont think there is any
alternative to a controllable ln2-cooled stage. Working nearer room temp
with a Peltier stage at higher chamber water pressures probably wouldnt help
very much either, since you would be balancing condensation/sublimation...

good luck!

Sally Stowe


Dr Sally Stowe
Facility Coordinator, ANU EMU
Box 475 Canberra ACT 2601
AUSTRALIA
stowe-at-rsbs.anu.edu.au fax 61 (0)2 6249 3218 or 6279 8525

} } } Bob Wise {wise-at-vaxa.cis.uwosh.edu} 08/24/00 03:09am } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


I would like to try to visualize ice in frozen plant leaves in a Hitachi
2460 variable pressure SEM and do not have access to a cold stage. I have
heard that a block of aluminum or copper at LN temperature may make a
usable substitute. Has anyone had any experience with this technique?
Any
and all tips would be appreciated.

TIA

Bob
Dr. Robert R. Wise
Associate Professor of Plant Physiology
Department of Biology
University of Wisconsin Oshkosh
Oshkosh, WI 54901
(920) 424-3404
wise-at-uwosh.edu
http://www.uwosh.edu/departments/biology/wise/wise.html




From daemon Thu Aug 24 10:27:16 2000



From: David L. Spector :      spector-at-cshl.org
Date: Wed, 23 Aug 2000 11:41:26 -0400
Subject: Position Available/Microscopy Core Technician

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Microscopy Core Technician.

Cold Spring Harbor Laboratory is seeking an experienced and responsible
microscopy technician for the laboratories core microscopy facility.
The individual should have practical expertise in transmission electron
microscopy, confocal and widefield fluorescence microscopy, digital
imaging, and microinjection. The successful candidate will be involved
in designing and carrying out experimental protocols for users, training
individuals in the use of various microscopes, and aligning microscopes
and keeping the facility operating at an efficient and high level of
productivity. Interested individuals should send their resume,
including a description of their expertise and the names and addresses
of 3 references to: Dr. David L. Spector, Cold Spring Harbor Laboratory,
One Bungtown Road, Cold Spring Harbor, New York 11724, email:
spector-at-cshl.org






From daemon Thu Aug 24 14:47:03 2000



From: Timothy Schneider :      Timothy.Schneider-at-Mail.TJU.EDU
Date: Thu, 24 Aug 2000 14:44:23 -0400
Subject: MORE CAMERAS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello:

I to was very impressed with the AMT, SIS, and Gatan cameras at the M&M
2000. I have a 1986 Hitachi 7000 STEM and would like to know if any one out
there has any digital experience with this make and model. Thanks, Tim

Timothy G. Schneider
Director of Electron Microscopy
Department of Pathology
Room 229 Jefferson Hall
Thomas Jefferson University
1020 Locust St.
Philadelphia Pa. 19107
215-503-4798 work
610-613-8170 cellular
timothy.schneider-at-mail.tju.edu




From daemon Thu Aug 24 15:24:40 2000



From: Erik Wilson :      wilsone-at-cae.wisc.edu
Date: Thu, 24 Aug 2000 15:24:48 -0500
Subject: Focused Ion Beam

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,

I am looking for a introductory technical reference for the Focused Ion
Beam (FIB) technique.
A journal article or textbook section would be useful. Can you suggest a
comprehensive
source?

Thank you,

Erik Wilson




From daemon Thu Aug 24 15:41:36 2000



From: Ladd Research :      ladres-at-worldnet.att.net
Date: Thu, 24 Aug 2000 16:32:04 -0400
Subject: Re: GA

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


After consulting with Dr. Garber, we are in general agreement on his
position concerning shipping and storing of GA. We believe adding cold
shipping requirements are overkill, but at the same token if any of our
customers feel it is necessary we are glad to oblige.
We have a long history of producing highly purified GA and in forty some
years have never had a problem with our guarantee of one year at RT.
There are conditions that could merit special packaging and shipping and
we would be glad to conform to any requests, but webelieve it to be an
unnecessary expense for most people.

John Arnott
Chairman

--

LADD RESEARCH
131 Dorset Lane
Williston, VT 05495 USA

TEL 1-800-451-3406 (US) or 1-802-878-6711 (anywhere)
FAX 1-802-878-8074
e-mail ladres-at-worldnet.att.net

ALL NEW WEB SITE UP NOW AT OUR NEW URL:

http://www.laddresearch.com

Microscope Supplies Since 1955


} } Jim Darley wrote:
} } =============================================================
} } Just to make the "GA is old" point a little clearer:
} } GA polymerises with time and higher temperatures; a nitrogen head does
not
} } change much. Frozen, GA will last an awfully long time and if stored
} } refrigerated most people would still be happy with the product after six
} } months
} } and more. While in the lab storage should not be a problem, we can be
sure
} } that
} } inappropriate storage is common.
} } The problem is in transit, when during the summer months the GA could
see
} } some
} } rather high temperatures during a truck or even an air journey. We have
} } always
} } taken some care with GA, but being a long way from the manufacturer we
have
} } adopted special precautions such as shipping with ice and mostly by air
and
} } incoming shipments are also packed in ice and refrigerated whenever not
in
} } the
} } air. Its costly, but this assures a better product.
} } (snip)
} } ==============================================================



From daemon Thu Aug 24 17:10:37 2000



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Thu, 24 Aug 2000 18:07:53 -0500
Subject: Glutaraldehyde: Shipment/Storage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Jim Darley wrote:
=============================================================
Just to make the "GA is old" point a little clearer:
GA polymerises with time and higher temperatures; a nitrogen head does not
change much. Frozen, GA will last an awfully long time and if stored
refrigerated most people would still be happy with the product after six
months
and more. While in the lab storage should not be a problem, we can be sure
that
inappropriate storage is common.
The problem is in transit, when during the summer months the GA could see
some
rather high temperatures during a truck or even an air journey. We have
always
taken some care with GA, but being a long way from the manufacturer we have
adopted special precautions such as shipping with ice and mostly by air and
incoming shipments are also packed in ice and refrigerated whenever not in
the
air. Its costly, but this assures a better product.
(snip)
==============================================================
Sometimes good technical people have honest differences of opinion. I am
sure this is one of those instances.

I have always been led to believe that the mechanism of ageing of GA is
related to the formation first of dimers and trimers of the monomer (it is
an autocatalytic reaction), and that the way to ensure longest shelf life is
to bring the starting purity down to levels (actually lower than I believe
most EM users would need) such that one can really delay (but not
indefinitely) the onset of the dimer/trimer reaction from starting. By
removing the existing dimers and trimers, you remove that which starts the
aging process.

When GA is purified to these levels, one can delay the onset of the aging
process to such a degree, that some producers of EM glutaraldehyde guarantee
their product to be good for one year at room temperature storage. SPI is
one of those and as of today, I reconfirmed that the Ladd glutaraldehyde is
in this category as well. Quite possibly, if not probably, there are other
vendors making EM glut at that same level of purity.

But with glutaraldehyde being purified to these levels, and with these
guarantees from the manufacturer for one year at room temperature, to
require cold shipment in ice, or even the use of frozen gel packs (which
usually are cheaper) in our opinion, is normally not required. Naturally,
upon arrival, we do recommend refrigerated storage, just out of general
principles, which might be more the result of "dogma" than real reasons.
But we ship regularly the ampouled GA to tropical environments,
unrefrigerated, and don't have any particular problems. We would
discourage shipment to tropical environments via air freight, since it could
end up sitting in customs for days on end but use of the courier services
seems to result in no difficulties.

Now for GA being sold in screw top bottles, this would be another story. If
the starting purity is good as indicated above, then refrigerated shipment
should not, in general be necessary. However, we would strongly recommend
the refrigerated storage upon arrival, indeed we think this should be a
requirement. The guarantees for room temperature storage for one year do
not extend to the product sold in the screw top bottles, only in the sealed
glass ampoules. We don't know about frozen storage, but usually most users
want their product in ready-to-use form and don't want to wait for something
to thaw out before using.

Again, different people have different opinions about these matters, and the
purpose of this posting is only to point out that there are these
differences, and that we do not believe one needs to regularly incur the
extra costs that would otherwise be necessary for the refrigerated shipment
of GA.

Chuck

Disclaimer: SPI Supplies offers EM grade glutaraldehyde to EM laboratories
worldwide and we do not refrigerate our shipments of GA and to my knowledge,
we have never had a customer receive GA that has gone bad in shipment.

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.spi.cc
########################
============================================









From daemon Thu Aug 24 22:24:02 2000



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Thu, 24 Aug 2000 17:06:17 -1000 (HST)
Subject: Clear embedding resin for display

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Colleagues-

Someone has asked what resin to use that would be clear and suitable for
embedding mouse embryos. This would be for display, not for sectioning.

Mahalo!
Tina

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************




From daemon Thu Aug 24 23:21:38 2000



From: jim :      jim-at-proscitech.com.au
Date: Fri, 25 Aug 2000 14:12:21 +1000
Subject: RE: Glutaraldehyde: Shipment/Storage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have never heard of those dimmers and trimmers, just seems a change from
various polymers and co-polymers. More importantly, I do not believe that
Chuck's supplier has something better than vacuum distillation available to
achieve highest purity. Not that I would want to get into the game of "mine is
purer than yours", since I have no doubt that at production either of the three
(to my knowledge) North American "refiners" produce the very best GA possible
and they are comparable.
The main points of this message are that:
1 For infusion and I understand cyto or immunocytochemistry the purest possible
GA should be used. I have read the suggestion that a small amount of polymer
may be good during conventional fixation, as this would impede osmotic shock.
2 I certainly do not agree with Chucks suggestions that GA may be stored at
room temperature for a year and then used for EM fixation - regardless of
initial purity. GA is not in this regard to be confused with the high purity
Formaldehyde (16%), which does not require refrigeration, keeps for years and
is an excellent fixative. That item come after Chuck's "snip". Hmmm.
3 The point of cold shipping that I had made was particularly pertinent to our
situation were the material needs to travel a long distance. But especially
during our summer months (and the USA can be just as hot) I believe that
shipping GA with some ice is worth the extra cost.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Friday, August 25, 2000 9:08 AM, Garber, Charles A. [SMTP:cgarber-at-2spi.com]
wrote:
}
} -- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --
}
} Jim Darley wrote:
} =============================================================
} Just to make the "GA is old" point a little clearer:
} GA polymerises with time and higher temperatures; a nitrogen head does not
} change much. Frozen, GA will last an awfully long time and if stored
} refrigerated most people would still be happy with the product after six
} months
} and more. While in the lab storage should not be a problem, we can be sure
} that
} inappropriate storage is common.
} The problem is in transit, when during the summer months the GA could see
} some
} rather high temperatures during a truck or even an air journey. We have
} always
} taken some care with GA, but being a long way from the manufacturer we have
} adopted special precautions such as shipping with ice and mostly by air and
} incoming shipments are also packed in ice and refrigerated whenever not in
} the
} air. Its costly, but this assures a better product.
} (snip)
} ==============================================================
} Sometimes good technical people have honest differences of opinion. I am
} sure this is one of those instances.
}
} I have always been led to believe that the mechanism of ageing of GA is
} related to the formation first of dimers and trimers of the monomer (it is
} an autocatalytic reaction), and that the way to ensure longest shelf life is
} to bring the starting purity down to levels (actually lower than I believe
} most EM users would need) such that one can really delay (but not
} indefinitely) the onset of the dimer/trimer reaction from starting. By
} removing the existing dimers and trimers, you remove that which starts the
} aging process.
}
} When GA is purified to these levels, one can delay the onset of the aging
} process to such a degree, that some producers of EM glutaraldehyde guarantee
} their product to be good for one year at room temperature storage. SPI is
} one of those and as of today, I reconfirmed that the Ladd glutaraldehyde is
} in this category as well. Quite possibly, if not probably, there are other
} vendors making EM glut at that same level of purity.
}
} But with glutaraldehyde being purified to these levels, and with these
} guarantees from the manufacturer for one year at room temperature, to
} require cold shipment in ice, or even the use of frozen gel packs (which
} usually are cheaper) in our opinion, is normally not required. Naturally,
} upon arrival, we do recommend refrigerated storage, just out of general
} principles, which might be more the result of "dogma" than real reasons.
} But we ship regularly the ampouled GA to tropical environments,
} unrefrigerated, and don't have any particular problems. We would
} discourage shipment to tropical environments via air freight, since it could
} end up sitting in customs for days on end but use of the courier services
} seems to result in no difficulties.
}
} Now for GA being sold in screw top bottles, this would be another story. If
} the starting purity is good as indicated above, then refrigerated shipment
} should not, in general be necessary. However, we would strongly recommend
} the refrigerated storage upon arrival, indeed we think this should be a
} requirement. The guarantees for room temperature storage for one year do
} not extend to the product sold in the screw top bottles, only in the sealed
} glass ampoules. We don't know about frozen storage, but usually most users
} want their product in ready-to-use form and don't want to wait for something
} to thaw out before using.
}
} Again, different people have different opinions about these matters, and the
} purpose of this posting is only to point out that there are these
} differences, and that we do not believe one needs to regularly incur the
} extra costs that would otherwise be necessary for the refrigerated shipment
} of GA.
}
} Chuck
}
} Disclaimer: SPI Supplies offers EM grade glutaraldehyde to EM laboratories
} worldwide and we do not refrigerate our shipments of GA and to my knowledge,
} we have never had a customer receive GA that has gone bad in shipment.
}
} ============================================
}
} Charles A. Garber, Ph. D. Ph: 1-610-436-5400
} President 1-800-2424-SPI
} SPI SUPPLIES FAX: 1-610-436-5755
} PO BOX 656 e-mail:cgarber-at-2spi.com
} West Chester, PA 19381-0656 USA
} Cust.Service: spi2spi-at-2spi.com
}
} Look for us!
} ########################
} WWW: http://www.spi.cc
} ########################
} ============================================
}
}
}
}
}
}
}




From daemon Fri Aug 25 01:16:31 2000



From: Erasmus, Willem (WJ) :      willem.erasmus-at-sasol.com
Date: Fri, 25 Aug 2000 08:06:27 +0200
Subject: File download problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Microscopists

Thanks to everyone who answered my query about the Topographic Measurement
paper by Dr John Russ and for all the helpful suggestions. The problem seems
to have fixed itself. This morning, after about 10 more attempts, I was
suddenly able to download the file. I have no idea what the problem was, and
neither does our IT department. It certainly wasn't the Adobe Acrobat Reader
or Internet Explorer. I have learned long ago that computers are strange
things, and this just proves it again !!!

Thanks
Willem Erasmus

Willem Erasmus
Snr. Scientist, Basic Catalysis Research
Sasol Technology
Tel : +27 +16 9603954
Fax : +27 +16 9602826
E-mail : willem.erasmus-at-sasol.com




From daemon Fri Aug 25 03:13:17 2000



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Fri, 25 Aug 2000 09:04:15 +0100 (GMT Daylight Time)
Subject: Re: Glutaraldehyde: Shipment/Storage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We store 25% glutaraldehyde in ampoules in the freezer
compartment of a domestic fridge . It does not freeze at
this temperature and is therefore ready for use very
quickly.

Dave


On Thu, 24 Aug 2000 18:07:53 -0500 "Garber, Charles A."
{cgarber-at-2spi.com} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} -- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --
}
} Jim Darley wrote:
} =============================================================
} Just to make the "GA is old" point a little clearer:
} GA polymerises with time and higher temperatures; a nitrogen head does not
} change much. Frozen, GA will last an awfully long time and if stored
} refrigerated most people would still be happy with the product after six
} months
} and more. While in the lab storage should not be a problem, we can be sure
} that
} inappropriate storage is common.
} The problem is in transit, when during the summer months the GA could see
} some
} rather high temperatures during a truck or even an air journey. We have
} always
} taken some care with GA, but being a long way from the manufacturer we have
} adopted special precautions such as shipping with ice and mostly by air and
} incoming shipments are also packed in ice and refrigerated whenever not in
} the
} air. Its costly, but this assures a better product.
} (snip)
} ==============================================================
} Sometimes good technical people have honest differences of opinion. I am
} sure this is one of those instances.
}
} I have always been led to believe that the mechanism of ageing of GA is
} related to the formation first of dimers and trimers of the monomer (it is
} an autocatalytic reaction), and that the way to ensure longest shelf life is
} to bring the starting purity down to levels (actually lower than I believe
} most EM users would need) such that one can really delay (but not
} indefinitely) the onset of the dimer/trimer reaction from starting. By
} removing the existing dimers and trimers, you remove that which starts the
} aging process.
}
} When GA is purified to these levels, one can delay the onset of the aging
} process to such a degree, that some producers of EM glutaraldehyde guarantee
} their product to be good for one year at room temperature storage. SPI is
} one of those and as of today, I reconfirmed that the Ladd glutaraldehyde is
} in this category as well. Quite possibly, if not probably, there are other
} vendors making EM glut at that same level of purity.
}
} But with glutaraldehyde being purified to these levels, and with these
} guarantees from the manufacturer for one year at room temperature, to
} require cold shipment in ice, or even the use of frozen gel packs (which
} usually are cheaper) in our opinion, is normally not required. Naturally,
} upon arrival, we do recommend refrigerated storage, just out of general
} principles, which might be more the result of "dogma" than real reasons.
} But we ship regularly the ampouled GA to tropical environments,
} unrefrigerated, and don't have any particular problems. We would
} discourage shipment to tropical environments via air freight, since it could
} end up sitting in customs for days on end but use of the courier services
} seems to result in no difficulties.
}
} Now for GA being sold in screw top bottles, this would be another story. If
} the starting purity is good as indicated above, then refrigerated shipment
} should not, in general be necessary. However, we would strongly recommend
} the refrigerated storage upon arrival, indeed we think this should be a
} requirement. The guarantees for room temperature storage for one year do
} not extend to the product sold in the screw top bottles, only in the sealed
} glass ampoules. We don't know about frozen storage, but usually most users
} want their product in ready-to-use form and don't want to wait for something
} to thaw out before using.
}
} Again, different people have different opinions about these matters, and the
} purpose of this posting is only to point out that there are these
} differences, and that we do not believe one needs to regularly incur the
} extra costs that would otherwise be necessary for the refrigerated shipment
} of GA.
}
} Chuck
}
} Disclaimer: SPI Supplies offers EM grade glutaraldehyde to EM laboratories
} worldwide and we do not refrigerate our shipments of GA and to my knowledge,
} we have never had a customer receive GA that has gone bad in shipment.
}
} ============================================
}
} Charles A. Garber, Ph. D. Ph: 1-610-436-5400
} President 1-800-2424-SPI
} SPI SUPPLIES FAX: 1-610-436-5755
} PO BOX 656 e-mail:cgarber-at-2spi.com
} West Chester, PA 19381-0656 USA
} Cust.Service: spi2spi-at-2spi.com
}
} Look for us!
} ########################
} WWW: http://www.spi.cc
} ########################
} ============================================
}
}
}
}
}
}
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"




From daemon Fri Aug 25 06:10:50 2000



From: J.Bruyntjes-at-voeding.tno.nl
Date: Fri, 25 Aug 2000 13:00:40 +0200
Subject: Silver deposits

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi there

Is anyone familiar with the staining of silver-deposits in rat-tissues.
Organs which may be involved are liver and kidneys. Is a silver-deposit
within tissue possible?


Joost Bruijntjes
TNO Nutrition and Food Research
Zeist
Holland




From daemon Fri Aug 25 08:07:50 2000



From: Manoj Misra :      Manoj.Misra-at-unilever.com
Date: Fri, 25 Aug 2000 08:59:11 -0400
Subject: TEM Position Available in Industry

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Exciting Opportunity for an Experienced Trasmission Electron Microscopist
in Advanced Imaging and Measurement, Unilever Research, Edgewater, NJ

Position Requirements:

A full time position is immediately available for a person experienced in
transmission electron microscopy of biological samples. This person will
be responsible for all aspects of tissue processing, sectioning, TEM
image acquisition as well as general laboratory maintenance. At least
a bachelors degree in biology is required with five years of experience in
bio-TEM. Previous experience in immuno-labeling, digital data acquisition
and computer image processing is desirable.

Opportunity exists for the person to expand skills and capabilities
in cryo-aplications (high pressure freezeing, cryo-ultramicrotomy,
freze substitution/fracture etc) and environmental scanning electron
microscopy.

Unilever is a leading consumer products company with world wide sales exceeding
$40 billion. To support our global business, Unilever has six international
research center
employing over 4000 scientists. Unilever's U.S. laboratory employs over 300
scientists
and is the designated headquarters for leading personal wash and skin care
research.
We are located on the Hudson River in Edgewater, New Jersey, within 20 minutes
of
midtown Manhattan with its diverse entertainment, arts and cultural resources.
We offer a
competitive salary, flexible benefits and excellent opportunities for both
personal and
professional growth. For more information about Unilever Research and
Unilever
visit our Web Site at http://www.unilever.com

If interested, please send resume to:

Manoj Misra Ph.D.
Group Head
Advanced Imaging and Measurement
Unilever Research
Edgewater, NJ 07020
E Mail: manoj.misra-at-unilever.com

(201) 840-2702 (voice)
(201) 840-8269 (Fax)



From daemon Fri Aug 25 08:45:29 2000



From: Philip Oshel :      peoshel-at-facstaff.wisc.edu
Date: Fri, 25 Aug 2000 08:42:59 -0500
Subject: Re: Glutaraldehyde: Shipment/Storage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


What influence does the concentration of glutaraldehyde have on its
shelf life? In the 'frig or at room temperature.

I've always thought that the higher % glut -- especially 70% -- was
more likely to polymerize, and that 25% or lower had the longest
shelf life. True or false?

I am assuming glutaraldehyde stored under nitrogen in sealed ampoules.

Phil

} -- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --
}
} Jim Darley wrote:
} =============================================================
} Just to make the "GA is old" point a little clearer:
} GA polymerises with time and higher temperatures; a nitrogen head does not
} change much. Frozen, GA will last an awfully long time and if stored
} refrigerated most people would still be happy with the product after six
} months
} and more. While in the lab storage should not be a problem, we can be sure
} that
} inappropriate storage is common.
} The problem is in transit, when during the summer months the GA could see
} some
} rather high temperatures during a truck or even an air journey. We have
} always
} taken some care with GA, but being a long way from the manufacturer we have
} adopted special precautions such as shipping with ice and mostly by air and
} incoming shipments are also packed in ice and refrigerated whenever not in
} the
} air. Its costly, but this assures a better product.
} (snip)
} ==============================================================
} Sometimes good technical people have honest differences of opinion. I am
} sure this is one of those instances.
}
} I have always been led to believe that the mechanism of ageing of GA is
} related to the formation first of dimers and trimers of the monomer (it is
} an autocatalytic reaction), and that the way to ensure longest shelf life is
} to bring the starting purity down to levels (actually lower than I believe
} most EM users would need) such that one can really delay (but not
} indefinitely) the onset of the dimer/trimer reaction from starting. By
} removing the existing dimers and trimers, you remove that which starts the
} aging process.
}
} When GA is purified to these levels, one can delay the onset of the aging
} process to such a degree, that some producers of EM glutaraldehyde guarantee
} their product to be good for one year at room temperature storage. SPI is
} one of those and as of today, I reconfirmed that the Ladd glutaraldehyde is
} in this category as well. Quite possibly, if not probably, there are other
} vendors making EM glut at that same level of purity.
}
} But with glutaraldehyde being purified to these levels, and with these
} guarantees from the manufacturer for one year at room temperature, to
} require cold shipment in ice, or even the use of frozen gel packs (which
} usually are cheaper) in our opinion, is normally not required. Naturally,
} upon arrival, we do recommend refrigerated storage, just out of general
} principles, which might be more the result of "dogma" than real reasons.
} But we ship regularly the ampouled GA to tropical environments,
} unrefrigerated, and don't have any particular problems. We would
} discourage shipment to tropical environments via air freight, since it could
} end up sitting in customs for days on end but use of the courier services
} seems to result in no difficulties.
}
} Now for GA being sold in screw top bottles, this would be another story. If
} the starting purity is good as indicated above, then refrigerated shipment
} should not, in general be necessary. However, we would strongly recommend
} the refrigerated storage upon arrival, indeed we think this should be a
} requirement. The guarantees for room temperature storage for one year do
} not extend to the product sold in the screw top bottles, only in the sealed
} glass ampoules. We don't know about frozen storage, but usually most users
} want their product in ready-to-use form and don't want to wait for something
} to thaw out before using.
}
} Again, different people have different opinions about these matters, and the
} purpose of this posting is only to point out that there are these
} differences, and that we do not believe one needs to regularly incur the
} extra costs that would otherwise be necessary for the refrigerated shipment
} of GA.
}
} Chuck
}
} Disclaimer: SPI Supplies offers EM grade glutaraldehyde to EM laboratories
} worldwide and we do not refrigerate our shipments of GA and to my knowledge,
} we have never had a customer receive GA that has gone bad in shipment.
}
} ============================================
}
} Charles A. Garber, Ph. D. Ph: 1-610-436-5400
} President 1-800-2424-SPI
} SPI SUPPLIES FAX: 1-610-436-5755
} PO BOX 656 e-mail:cgarber-at-2spi.com
} West Chester, PA 19381-0656 USA
} Cust.Service: spi2spi-at-2spi.com
}
} Look for us!
} ########################
} WWW: http://www.spi.cc
} ########################
} ============================================

--
}}}}}}}}}}}}}}}}}{{{{{{{{{{{{{{{{{
Philip Oshel
Supervisor, AMFSC and BBPIC
Dept. of Animal Health and Biomedical Sciences
University of Wisconsin
1656 Linden Drive
Madison, WI 53706-1581
voice: (608) 263-4162
fax: (608) 262-7420 (dept. fax)



From daemon Fri Aug 25 10:51:45 2000



From: SGKCCK-at-aol.com
Date: Fri, 25 Aug 2000 11:40:56 EDT
Subject: Glut.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello to all,

Another 2 cents worth. I have sat back and read with enjoyment all of the
bantering regarding the facts and fiction on Glut and cannot believe that
some people are actually arguing about issues which are so well cited in the
literature and this argument is going to lead to the confusion of many
researchers with no basis at all.
The truth on Glut is short and simple and I do not care who is making it the
facts remain the same no matter. Please see the facts below. Anyone whom
would like to question these results I remain at your disposal to open my lab
notebooks and results of all of the Glutaraldehyde in the industry today and
its corresponding results. There are only 3 manufacturers of the final
distilled product in the US and each and every one of them shows the same
tendency towards temp vs time.

GLUTARALDEHYDE STORAGE ISSUES

A key detail in storage analysis for this product is that EM grade GA is
usually supplied as "aqueous solution" of various concentrations and not as
"pure", and the "pure iron won't rust" principle simply does not apply.
This aqueous chemical system is well known to be extremely active,
especially at temperatures exceeding +40 C (because of common chemical
knowledge reasons, pertaining to water molecules intrinsic association
tendency, on one hand, and to the particular GA_dimer polarisation of
hydrating aggregates, on the other hand).
As many EM users already know, GA polimerizes under the influence of
traces of impurities, the process belonging to singlet-triplet activation
mechanism (i.e., temperature, pH, concentration highly sensitive), and by no
means is it an "autocatalytic" one. Traces of water (along with acrolein,
ethanol, methanol, pyranic compounds), and a slight azeotropic tendency, lead
ultimately to the formation of glutaric acid and consequently to the drastic
decrease of pH, making GA improper for EM usage, as in: Hayat, M.A.,
"Principles And Techniques of Electron Microscopy", 2000. In those new
created conditions, GA quickly reversibly turns to several polymeric species,
some of them obviously detectable, some not. Alkaline artificially buffered
solutions cannot prevent polymerisation either, and the process starts with a
GA_trimer, as in: Fahimi, HD., et.al., "Essais de standardisation de la
fixation en glutaralgehyde", J. Microsc. (Paris, France), 4: 725-736, 1965
(!!!)
In time, several important kinetic stability studies were performed upon
EM grade GA. For general information, and, for most of readers, as a kind
reminder, we had selected conclusions made public in Histochemie, 30, 1972
(!!!), by Gillet, R., et.al., from Queen Elizabeth College, London, England:
"if the absorbance"..."is plotted against storage temperature, a
direct
relationship is obtained between storage temperature (above 00 C)
and the appearance of"... spectral ... "absorbing material".

(Please see graph attached.)


The authors wrote: "the most important storage criterion was
definitely temperature. Those samples stored at -200 C showed
virtually no change in"..."absorbance characteristics, even after
eight
months storage"
..."all samples stored at +200 C, however, showed considerable
absorbance peaks"..."the effect of storage at +370 C"..."was even
more pronounced."
It appears very speculative and pragmatic to neglect such important
facts, or categorize them as "dogmas". Certain manufacturers, who did not
receive any complaints (yet) when delivering room temperature stored GA,
should consider themselves extremely lucky, and should scrupulously revise
their knowledge about EMS grade GA, regardless if sold in ampoules or screw
cap bottles.

I look forward to hearing from anyone whom does not agree.

All of the very best
Stacie Kirsch
Electron Microscopy Sciences
215-646-1566



From daemon Fri Aug 25 14:26:08 2000



From: Dmitri Lapotko :      ld-at-hmti.ac.by
Date: Fri, 25 Aug 2000 22:18:05 +0300
Subject: LM: microscope survey

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hi Group,

Please advice - I desperately need a source of info for comparative
analysis of main existing microscope types - standard transmittance
microscope,phase-contrast, interference, fluorescent, confocal, laser
scanning ...in terms of their baic parameters:
spatial resolution, sensitivity to light absorption, temporal resolution.
Please advice a web-source or some other on-line esource if possible
- in case of book/article I will not have time for ordering.

Thanks

Dmitri Lapotko,

Luikov Heat and Mass Transfer Institute
Minsk
Ld-at-hmti.ac.by




From daemon Fri Aug 25 14:37:00 2000



From: Beavers, Roy :      rbeavers-at-post.cis.smu.edu
Date: Fri, 25 Aug 2000 14:14:50 -0500
Subject: Cutting Bone

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Not sure if this is the right forum to help me with this issue but thought
I would give it a try. I have a student who wants to cut bone (non fossil)
samples. She is not necessarily making thin sections but wants a cut better
than can be done with a coping saw. I offered my Buehler Isomet low speed
saw with a diamond cut off blade but that seem to be a bit too slow and
awkward with the larger bones. Looking for ideas.

Thanks

Roy Beavers
Southern Methodist University
Dept. of Geological Sciences
Electron Microprobe Lab
P.O. Box 750395
Dallas, Tx 75275
voice: 214-768-2756
fax: 214-768-2701
E-mail: rbeavers-at-mail.smu.edu




From daemon Fri Aug 25 14:47:26 2000



From: L R MELSEN :      lmelsen-at-emory.edu
Date: Fri, 25 Aug 2000 15:35:12 -0400
Subject: glutaraldehyde

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Being an old timer, I have been around since before the advent of vials
of GA. In the 70's the Merck index described a method for purifying GA.
using activated charcoal.

Basically GA begins to degrade to "multimers" when stored at room
temperature. The best fixation is achieved with the monomeric form,
therefore the purification protocol was designed to remove the more
complex forms.

Storage under nitrogen in a sealed vial slows the degradation. Lower
concentration degrade more slowly. Storing at 4C is advised.

Method:
Mix the concentrated liquid glutaraldehyde (25%) with 1g of activated
charcoal per 100 ml of liquid.
Allow to stand for 30 minutes, then vacuum filter through a Whatman #1
filter.
Repeat three times.
Store at 4C
Repeat once a year.

I have used this method for more than 30 years and consistently obtained
very satisfactory fixation. It is messy, but worth the effort.




From daemon Fri Aug 25 15:10:26 2000



From: Geoff McAuliffe :      mcauliff-at-UMDNJ.EDU
Date: Fri, 25 Aug 2000 16:03:30 -0400
Subject: Drosophila sectioning

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear List:

I am helping, or trying to help, a colleague with sectioning of
Drosophila legs. They are embedded in "Immunobed" (a methycrylate) or
Epon (ok, Epon substitute). I don't seem to be getting a good bond
between the chitin and the plastic, the tissue falls out. Any
suggestions are welcome.

Geoff
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************





From daemon Fri Aug 25 15:40:22 2000



From: Paul Webster :      pwebster-at-mailhouse.hei.org
Date: 25 Aug 00 13:38:43 -0700
Subject: EM:LKB grid stainer

Contents Retrieved from Microscopy Listserver Archives
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------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Hello,

We inherited an old LKB grid stainer that has worked well for nearly two years. Now it has developed a leak.

Is there anyone who has one of these machines that they keep for spares (or to hold the door open)? I need the spindle unit for one of the pump/valve assemblies. The housing looks fine so it should be easy enough to plug in a spare unit.

For anyone who has not seen inside this machine, the above description must seem very strange. My apologies for this. My hope is that someone who has seen inside can understand my description and has the part I need.

I have already contacted Leica to see if they are still supporting this machine, so there is no need to recommend I contact them.

Thanks in advance for any help, suggestions, comments or any other e-mail you may send my way.

Paul Webster.

Paul Webster, Ph.D
House Ear Institute
2100 West Third Street
Los Angeles, CA 90057
phone:213 273 8026
fax: 213 413 6739
e-mail: pwebster-at-hei.org
http://www.hei.org/htm/aemi.htm




From daemon Fri Aug 25 15:56:07 2000



From: Philip Oshel :      peoshel-at-facstaff.wisc.edu
Date: Fri, 25 Aug 2000 15:53:52 -0500
Subject: Re: Cutting Bone

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Roy,

If you don't get the answer from the microscopy group, you might try Histonet:
Histonet-at-Pathology.swmed.edu
to subscribe, put "subscribe" no quotes in the subject line, leave
the message body blank
unsubscribe the same way.
Spell these correctly! They use one address for everything, so
"suscribe" or "subscribe" with quotes gets sent to the list as a
message, not read as a command by the serverbot.
They have a bunch of folks who specialize in cutting bone, calcified
and decalcified. Their archives can be searched at:
http://www.histosearch.com/histonet.html

Otherwise, does someone at SMU have a diamond wire saw? Those work
well for slicing intact bones. There's also a double-bladed hand bone
saw that makes nice slices. Someone at the SMU med school or hospital
ought to have one.

Phil

} Not sure if this is the right forum to help me with this issue but thought
} I would give it a try. I have a student who wants to cut bone (non fossil)
} samples. She is not necessarily making thin sections but wants a cut better
} than can be done with a coping saw. I offered my Buehler Isomet low speed
} saw with a diamond cut off blade but that seem to be a bit too slow and
} awkward with the larger bones. Looking for ideas.
}
} Thanks
}
} Roy Beavers
} Southern Methodist University
} Dept. of Geological Sciences
} Electron Microprobe Lab
} P.O. Box 750395
} Dallas, Tx 75275
} voice: 214-768-2756
} fax: 214-768-2701
} E-mail: rbeavers-at-mail.smu.edu

--
}}}}}}}}}}}}}}}}}{{{{{{{{{{{{{{{{{
Philip Oshel
Supervisor, AMFSC and BBPIC
Dept. of Animal Health and Biomedical Sciences
University of Wisconsin
1656 Linden Drive
Madison, WI 53706-1581
voice: (608) 263-4162
fax: (608) 262-7420 (dept. fax)



From daemon Fri Aug 25 18:50:58 2000



From: Gordon Couger :      gcouger-at-couger.com
Date: Fri, 25 Aug 2000 17:37:28 -0500
Subject: Re: Cutting Bone

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


If you have a machine shop cut the bone in to thin sections on a band saw
sand one side smooth and super glue the bone to a disk you can mount in a
lathe or a plate you can mount on a mill or grinder. Then use thin the
material as thin as you want with a grinder.

Acetone will turn them loose from the plate. You can do a bunch of
sections at a time on a big plate.

Embeding them before you grind them will help preserve fine structures.

Exactly how the machine shop does the work will depend on the tools they
have available.


Gordon
Gordon Couger gcouger-at-couger.com
Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00
-
} Not sure if this is the right forum to help me with this issue but
thought
} I would give it a try. I have a student who wants to cut bone (non
fossil)
} samples. She is not necessarily making thin sections but wants a cut
better
} than can be done with a coping saw. I offered my Buehler Isomet low
speed
} saw with a diamond cut off blade but that seem to be a bit too slow and
} awkward with the larger bones. Looking for ideas.

}
}





From daemon Sat Aug 26 08:19:34 2000



From: jim :      jim-at-proscitech.com.au
Date: Sat, 26 Aug 2000 20:36:14 +1000
Subject: RE: glutaraldehyde

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I too purified GA by charcoal filtering and even vacuum distillation; its over
25 years ago. As things were published I also played around with making thin
film apertures and I know that other people have made their own filaments and
even the making of a LaB6 is published in the literature, for the intrepid.
Don't think that I worry about loosing some business either, that too is not
one of my traits.
I like to make the following observations on purifying GA, we keep cathodes for
another day:
Charcoal filtered GA is not as good the commercial vacuum distilled product.
You should store it in a freezer (even a domestic fridge's') if its for more
then a few weeks.
The triple charcoal filtered product is good enough for EM fixation, if stored
well.
It's dubious that its good enough for cytochemistry.
Charcoal is a recognized carcinogen (funny that, it used to be prescribed
against diarrhea) its also expensive and a DG to ship.
Several ml of GA are lost in every filtration.
Any employer would cost an employee at at least 2x the hourly cost; in research
its easy to forget the real world, were time is money.
"Home filtered" GA is very expensive.

Different parameters may apply elsewhere, so in countries were the hourly wage
is 50 cents or less, and a drum of GA fell of a truck at a leather tanning or
paper towel factory (its used to soften leather and paper) and somebody is
making coconut charcoal down the road . . .
I would be into that and make my own purified GA too. But not in America!
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Saturday, August 26, 2000 5:35 AM, L R MELSEN [SMTP:lmelsen-at-emory.edu]
wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Being an old timer, I have been around since before the advent of vials
} of GA. In the 70's the Merck index described a method for purifying GA.
} using activated charcoal.
}
} Basically GA begins to degrade to "multimers" when stored at room
} temperature. The best fixation is achieved with the monomeric form,
} therefore the purification protocol was designed to remove the more
} complex forms.
}
} Storage under nitrogen in a sealed vial slows the degradation. Lower
} concentration degrade more slowly. Storing at 4C is advised.
}
} Method:
} Mix the concentrated liquid glutaraldehyde (25%) with 1g of activated
} charcoal per 100 ml of liquid.
} Allow to stand for 30 minutes, then vacuum filter through a Whatman #1
} filter.
} Repeat three times.
} Store at 4C
} Repeat once a year.
}
} I have used this method for more than 30 years and consistently obtained
} very satisfactory fixation. It is messy, but worth the effort.
}




From daemon Sat Aug 26 13:42:54 2000



From: Marek Malecki :      mmm-at-ucsd.edu
Date: Sat, 26 Aug 2000 13:29:59 -0500
Subject: Philips EM 300 available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


..We have decided as a department to close down our transmission EM
} } operation. We have a Philips EM 300 ... which we are willing to donate
to someone for parts, provided they pay for disassembly and shipping to
their site...


For details please contact:
} } } David Kerk, Ph.D.
} } } Professor and Chair
} } } Department of Biology
} } } Point Loma Nazarene University
} } } 3900 Lomaland Dr
} } } San Diego, CA 92106
} } } Ph: 619-849-2398
} } } FAX: 619-849-2598
} } } email: dkerk-at-ptloma.edu


M. Malecki, M.D., Ph.D.
Director and Principal Investigator

Molecular Imaging Laboratories
University of California at San Diego
address: 1500 Suite Bonner Hall, 9500 Gilman Drive, La Jolla, CA 92093-0368
phone: 8588223373
fax: 8588223715
pager: 8586161420
e.mail: mmm-at-ucsd.edu
e.pager: 1620024619-at-alphapage.airtouch.com
www site: http://mil.ucsd.edu/
ftp site: mil1.ucsd.edu





From daemon Sat Aug 26 13:48:17 2000



From: Marek Malecki :      mmm-at-ucsd.edu
Date: Sat, 26 Aug 2000 11:43:06 -0700
Subject: Fwd: glutaraldehyde - distillation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


As an alternative to charcoal purification of GA described by Dr. MELSEN, I
run distillation, collect the fractions, and then select the monomeric form
on the spec.



} Date: Fri, 25 Aug 2000 15:35:12 -0400
} From: L R MELSEN {lmelsen-at-emory.edu}
} Reply-To: lmelsen-at-emory.edu
} Organization: Emory University
} X-Mailer: Mozilla 4.73 (Macintosh; I; PPC)
} X-Accept-Language: en
} To: MICROSCOPY {Microscopy-at-sparc5.microscopy.com}
} Subject: glutaraldehyde
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


M. Malecki, M.D., Ph.D.
Director and Principal Investigator

Molecular Imaging Laboratories
University of California at San Diego
address: 1500 Suite Bonner Hall, 9500 Gilman Drive, La Jolla, CA 92093-0368
phone: 8588223373
fax: 8588223715
pager: 8586161420
e.mail: mmm-at-ucsd.edu
e.pager: 1620024619-at-alphapage.airtouch.com
www site: http://mil.ucsd.edu/
ftp site: mil1.ucsd.edu








From daemon Mon Aug 28 11:12:19 2000



From: David Henriks :      Henriks-at-CompuServe.COM
Date: Mon, 28 Aug 2000 13:27:55 -0400
Subject: Clear embedding resin for display

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Tina Carvalho wrote:

} Someone has asked what resin to use that would be clear and suitable for
} embedding mouse embryos. This would be for display, not for sectioning.
}

Dear Tina,
How about lucite? I have no idea where to get it, but many years ago I
bought a lucite cube in which a dandilion had been embedded. You could
see it very clearly, and there is obviously a technique for preserving such
a fragile specimen. Note that this is more along the lines of an existance
theorem than a procedure. Good luck.
Yours,
Bill Tivol




From root Mon Aug 28 13:15:45 2000
Return-Path: {Microscopy-request-at-sparc5.microscopy.com}
Received: (from daemon-at-localhost)
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Mon, 28 Aug 2000 13:28:12 -0400 (EDT)


Tina:

The best material to use in display type applications is a polyester
material. We have a material called PolyMet which is ideal. It takes a
while to cure - typically overnight - but it provides a crystal clear
mount. I hope this helps.

Best regards-

David
Writing at 10:07:06 AM on 08/28/2000

***************************************************************************
************************

David Henriks
Vice President TEL: 800-728-2233
(toll free in the USA)
South Bay Technology, Inc. +1-949-492-2600
1120 Via Callejon FAX: +1-949-492-1499
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

***************************************************************************
************************

} } } } } Please visit us at http://www.southbaytech.com { { { { {

Manufacturers of precision sample preparation equipment and supplies for
metallography, crystallography and electron microscopy.

Message text written by Tina Carvalho
}
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Colleagues-

Someone has asked what resin to use that would be clear and suitable for
embedding mouse embryos. This would be for display, not for sectioning.

Mahalo!
Tina

***************************************************************************
*
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu
*
* Biological Electron Microscope Facility * (808) 956-6251
*
* University of Hawaii at Manoa *
http://www.pbrc.hawaii.edu/bemf*
***************************************************************************
*

{



From daemon Mon Aug 28 18:26:49 2000



From: Ladd Research :      ladres-at-worldnet.att.net
Date: Mon, 28 Aug 2000 17:24:29 -0400
Subject: Glut

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Based on recent communications it appears there is a difference of
opinion on the shipping of refined Glut among the three major U.S.
refiners.

Ladd and a second refiner who has not been heard from do not add ice.
The third refiner does. When adding extras there is a higher cost and,
though we don't always acknowledge it, these costs do get passed on to
the consumer.

All three refiners know their specific product and their techniques and
make their decisions on that basis. If the refiner who adds ice feels
their product requires it I have great respect for that decision. They
know their product and know the best way to get it to their customer.

All data and history indicates Ladd glut does not require ice. If
special circumstances arise we would be glad to add ice.

We all act in good faith. If the refiner who adds ice is right and the
others are wrong I will acknowledge and applaud that decision. We do
add cold packaging for other chemicals like Mercox and LR White when
required.

John Arnott
--

LADD RESEARCH
131 Dorset Lane
Williston, VT 05495 USA

TEL 1-800-451-3406 (US) or 1-802-878-6711 (anywhere)
FAX 1-802-878-8074
e-mail ladres-at-worldnet.att.net

ALL NEW WEB SITE UP NOW AT OUR NEW URL:

http://www.laddresearch.com

Microscope Supplies Since 1955



From daemon Tue Aug 29 07:09:16 2000



From: Shafei, Mansour A :      shafeima-at-aramco.com.sa
Date: Tue, 29 Aug 2000 06:53:26 -0500
Subject: microwave oven

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,
One day someone said that microwave oven is very dangerous to be operated in
a level with the operator. Any advise, article in this matter?
Thanks
Mansour Al-Shafei
Senior Lab Sci.
Saudi Aramco Oil Company
Lab Research & Development Center
Analytical Sciences Division
Advanced Instruments Unit
Voice: 966-3-876-4360
E-mail: shafeima-at-aramco.com.sa





From daemon Tue Aug 29 08:19:19 2000



From: George_Munzing-at-ENGELHARD.COM
Date: Tue, 29 Aug 2000 09:04:28 -0400
Subject: Re: Clear embedding resin for display

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Tina Carvalho wrote:

} Someone has asked what resin to use that would be clear and suitable for
} embedding mouse embryos. This would be for display, not for sectioning.
}


Dear Tina,

There is also a product called Cast N' Craft casting resin available at
craft stores (Pearl, etc.) which I have had some success. You can not cast
under vacuum however you get a very clear, bubble free final product. For
spiders and smaller insects its been O.K but you may have difficulty with
any large objects. It's only a few bucks and may be worth a shot [provided
you have a few embryos to spare :)]. Good Luck.

Regards,

George R. Munzing Jr.






William Tivol {tivol-at-wadsworth.org} -at-wadsworth.org on 08/28/2000 11:42:03 AM

Sent by: tivol-at-wadsworth.org


To: microscopy-at-sparc5.microscopy.com
cc:


Tina Carvalho wrote:

} Someone has asked what resin to use that would be clear and suitable for
} embedding mouse embryos. This would be for display, not for sectioning.
}

Dear Tina,
How about lucite? I have no idea where to get it, but many years ago I
bought a lucite cube in which a dandilion had been embedded. You could
see it very clearly, and there is obviously a technique for preserving such
a fragile specimen. Note that this is more along the lines of an existance
theorem than a procedure. Good luck.
Yours,
Bill Tivol








From daemon Tue Aug 29 09:50:40 2000



From: Robert Hull :      rh5c-at-virginia.edu
Date: Tue, 29 Aug 2000 10:33:43 -0400
Subject: Postdoctoral position in in-situ electron microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


{html}
{font face="Times New Roman, Times"} A postdoctoral scientist is sought
for a joint research program between the University of Virginia and the
IBM Thomas J. Watson Research Center, centered upon real-time electron
microscope studies of the evolution of epitaxial semiconductor {br}
nanostructures. This position will be funded by an anticipated National
Science Foundation award to establish a Materials Research Science and
Engineering Center (MRSEC) at the University of Virginia. The successful
applicant will be an employee of the Department of Materials Science and
Engineering at the University of Virginia, but will spend the bulk of his
/ her time based at IBM, using the unique in-situ imaging and {br}
deposition tools in the Nanoscale Materials Analysis Department in the
Physical Sciences Division at the Thomas J. Watson Research Center. {br}
{br}
Applicants will require a PhD (or equivalent) in Materials Science,
Physics or a related discipline. The successful candidate will be able to
demonstrate strong background in electron microscopy, and will preferably
have expertise in ultra-high vacuum techniques. A research background in
electronic materials will also be a strong asset. {br}
{br}
Review of applications will begin on Sep 15, and will continue until the
position is filled.  The starting date will be in the October 
November 2000 timeframe.  The initial appointment will be for one
year, and will be extendible annually for an additional two years,
depending upon continuing funding. Applications should be addressed to:
Professor Robert Hull, Department of Materials Science and Engineering,
University of Virginia, 116 Engineers Way, Charlottesville, Virginia
22904. Please provide a curriculum vitae, copies of up to five recent
relevant publications, and contact information for three referees
familiar with your work and accomplishments. {br}
{br}
Additional information on the Department of Materials Science and
Engineering may be found at
{a href="http://www.mse.virginia.edu/" eudora="autourl"} http://www.mse.virginia.edu {/a} ,
and on the IBM Thomas J. Watson Research Center at
{a href="http://www.research.ibm.com/" eudora="autourl"} www.research.ibm.com {/a} .
The University of Virginia and IBM are Equal Opportunity / Affirmative
Action employers.  Applicants must be able to lawfully accept
employment in the United States. {br}
{br}
{/font}
{BR}
{br}
{div} Robert Hull {/div}
{div} Department of Materials Science and Engineering {/div}
{div} University of Virginia {/div}
{div} 116 Engineers Way {/div}
{div} Charlottesville, VA 22904-4745 {/div}
{br}
{div} Tel: 804-982-5658 {/div}
{div} FAX: 804-982-5660 {/div}
email:hull-at-virginia.edu
{/html}



From daemon Tue Aug 29 09:56:20 2000



From: jim :      jim-at-proscitech.com.au
Date: Wed, 30 Aug 2000 00:36:00 +1000
Subject: RE: microwave oven

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Modern microwave ovens are so well shielded that you cannot get a reading with
the sensitive meters that we supply. The only way you can show that the meter
is working is to place it within the microwave - for a few seconds.
I'd rather worry about gamma rays from outer space.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Tuesday, August 29, 2000 9:53 PM, Shafei, Mansour A
[SMTP:shafeima-at-aramco.com.sa] wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hello,
} One day someone said that microwave oven is very dangerous to be operated in
} a level with the operator. Any advise, article in this matter?
} Thanks
} Mansour Al-Shafei
} Senior Lab Sci.
} Saudi Aramco Oil Company
} Lab Research & Development Center
} Analytical Sciences Division
} Advanced Instruments Unit
} Voice: 966-3-876-4360
} E-mail: shafeima-at-aramco.com.sa
}
}




From daemon Tue Aug 29 10:07:25 2000



From: Robert Palmer :      rjpalmer-at-dir.nidcr.nih.gov
Date: Tue, 29 Aug 2000 10:57:00 -0500
Subject: freeze-fracture

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am seeking advice on freeze-fracture EM. I am particularly interested in
bacterial membranes and cell walls, and those who do immunolocalization on
these kinds of preparations.

Please contact me OFF LIST at: rjpalmer-at-dir.nidcr.nih.gov
Robert J. Palmer Jr., Ph.D.
Natl Inst Dental Craniofacial Res - Natl Insts Health
Oral Infection and Immunity Branch
Bldg 30, Room 308
30 Convent Drive
Bethesda MD 20892
ph 301-594-0025
fax 301-402-0396



From daemon Tue Aug 29 12:17:49 2000



From: Larry D. Ackerman :      mishot-at-itsa.ucsf.edu
Date: Tue, 29 Aug 2000 09:37:40 -0700
Subject: Clear embedding resin for display

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I recall using a resin called "BioPlastic" or something similar. I believe
it came from Wards and was designed for exactly the purpose you state.

Wards Scientific

http://www.wardsci.com/

1-800-962-2660
Fax: 1-800-635-8439
In Canada: 1-800-387-7822
International Customers: 1-716-359-2502
Fax: 1-716-334-6174


Larry D. Ackerman
Lily & Yuh Nung Jan Laboratories
Howard Hughes Medical Institute
UCSF, Box 0725, Rm U226
533 Parnassus Ave.
San Francisco, CA 94143

(415) 476-8751 FAX (415) 476-5774
mishot-at-itsa.ucsf.edu



From daemon Tue Aug 29 12:35:14 2000



From: David R Hull :      David.R.Hull-at-grc.nasa.gov
Date: Tue, 29 Aug 2000 13:21:37 -0400
Subject: TEM: Melting and Flow of Nanoparticles During TEM Observation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have been looking at the use of various catalysts for production
carbon nanotubes. Recently we had a sample that behaved in a very
unusual way. Upon focusing the electron beam on a cluster of
nanotubes, the catalyst particles became fluid and ran through the
tubes at about 50-100 nm/sec. I was a little surprised by the
movement. The catalyst particles would stop moving within 2-5
seconds.

I have found one reference, Buffat and Borel, Phys. Rev A13,
2287(1976), that showed the melting temperature of gold nanoparticles
could be reduced by 600C! for nanoparticles less than 5 nm.

In our case we have cobalt nanoparticles less than 10 nm.

My question is how high a temperature might we expect to heat the
sample in the electron beam with the following parameters;

200 kV, LaB6, 20-30 microamp emmission current, spot size 1, 150
micron condenser aperture.

Have others seen this phenomenon?
David R. Hull
NASA Glenn Research Center at Lewis Field
Advanced Metallics Branch
Mail Stop 49-1
21000 Brookpark Road
Cleveland, OH 44135

(216) 433-3281
fax (216)977- 7132
david.r.hull-at-grc.nasa.gov



From daemon Tue Aug 29 12:40:04 2000



From: jmkrupp-at-cats.ucsc.edu (Jon Krupp)
Date: Tue, 29 Aug 2000 10:27:35 -0700
Subject: EM Tech Position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am posting this for a colleague searching for an experienced EM tech.

----------------------------------------------------------------------------
-----


Electron Microscopist

A full time position for an experienced electron microscopist is available
in the laboratory of Yishi Jin at the University of California, Santa Cruz.
Dr. Jin studies the cytoskeletal architecture at synapses in the nervous
system of the nematode C. elegans. For more detailed information about the
work being done in the lab see
http://www.biology.ucsc.edu/faculty/jin.html, or consult these recent
publications; Zhen et al.2000.Neuron 26:331-343 or Zhen and Jin.1999.
Nature.41:371-375.

The person we seek must have prior experience in sample preparation for
transmission electron microscopy of biological materials such as fixation,
embedding, sectioning, staining, and photography. Proficiency in serial
sectioning would be an advantage, but is not essential at the time of
employment, nor is experience with C. elegans. The person selected must be
highly motivated, able to work independently, and be willing to acquire new
skills and techniques. A Bachelors's degree or equivalent in a biological
science is desirable.

This position is funded by the Howard Hughes Medical Institute. Salary will
be commensurate with experience within the range and criteria determined by
HHMI.

Interested individuals should send a current CV, names, addresses, and
email contact information of 3 references, and examples of previous EM work
to: Dr. Yishi Jin, Department of Biology, 328 Sinsheimer Laboratories,
University of California, Santa Cruz, CA 95064 by November 1, 2000. Dr.
Jin's email address is jin-at-biology.ucsc.edu.

-------------------------------------------------------------------

Jonathan Krupp
Microscopy & Imaging Lab
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu





From daemon Tue Aug 29 15:14:28 2000



From: Mick Thomas :      mgt3-at-ccmr.cornell.edu
Date: Tue, 29 Aug 2000 16:01:14 -0700
Subject: Link AN10000 problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Fellow microscopists,

We are still using a 15 year old Link AN10000 x-ray detector. The widths
of the peaks have suddenly become much wider. The zero beam or strobe used
to be around 80 eV FWHM, and now is around 360 eV, with no beam in the
column. To the best of my knowledge there is no light in the column, and
the dewar has plenty of LN2 in it, so I am assuming the crystal is
cold. Also, if I disconnect the Lemo connector (carrying the signal from
the detector) the FWHM drops to about 20 eV FWHM. If anyone has any
suggestions I would be very appreciative.

Thanks,

Mick Thomas
-------------------------------------------------
Mick Thomas
UHV-STEM Laboratory
E-1 Clark Hall
Cornell University
Ithaca, NY 14853

Phone: 607-255-0650
Fax: 607-255-7658
e-mail: mgt3-at-ccmr.cornell.edu



From daemon Tue Aug 29 15:26:38 2000



From: John J. Bozzola :      bozzola-at-siu.edu
Date: Tue, 29 Aug 2000 14:53:16 -0500
Subject: Olympus Confocal BF Question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


A colleague is trying to use an Olympus Fluoview CLSM System on a
BX50WI upright (not inverted) microscope. She is unable to obtain a
decent brightfield image. Question: Has anyone developed their own
set of operating directions that they would be willing to share?
Unfortunately, I have no experience with CLSM and can offer no
assistance to her.

Thank you.

JB
--
####################################################################
John J. Bozzola, Ph.D., Director
Micro-Imaging and Analysis Center
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
####################################################################



From daemon Tue Aug 29 18:32:34 2000



From: Purdy, Sam :      SPurdy-at-nationalsteel.com (by way of Nestor J. Zaluzec)
Date: Tue, 29 Aug 2000 18:17:30 -0500
Subject: RE: Clear embedding resin for display

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have had great successs with Castoliteto make very clear, water white
mounts of various natural materials. I have seen some with dandelion heads
clearly defined.

Castolite can be obtained from Buehler, Ltd. 41 Waukeegan Rd. PO box
1, Lake Bluff, IL, 60044-7979, 800-283-4537




} Tina Carvalho wrote:
}
} } Someone has asked what resin to use that would be clear and suitable for
} } embedding mouse embryos. This would be for display, not for sectioning.
} }
}
} Dear Tina,
} How about lucite? I have no idea where to get it, but many years ago
} I
} bought a lucite cube in which a dandilion had been embedded. You could
} see it very clearly, and there is obviously a technique for preserving
} such
} a fragile specimen. Note that this is more along the lines of an
} existance
} theorem than a procedure. Good luck.
} Yours,
} Bill Tivol
}
}
}
}
}
}





From daemon Tue Aug 29 18:52:11 2000



From: Jane Zhu :      jzhu-at-nmsu.edu
Date: Tue, 29 Aug 2000 17:42:38 -0600
Subject: Double tilt holder for Hitachi H7000

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


If you, or people you know, have a double tilt holder that can be used for
Hitachi H7000 to sale, please contact me or Dr. Soumitra Ghoshroy
(505-646-3600, sghoshro-at-nmsu.edu).
TIA.
----------------------------------------------------------------------------
-------------------
Prof. Jane G. Zhu
Department of Physics Phone: 505-646-1933
New Mexico State University Fax: 505-646-1934
MSC 3D, P.O. Box 30001 E-mail: jzhu-at-nmsu.edu
Las Cruces, NM 88003
----------------------------------------------------------------------------
-------------------




From daemon Tue Aug 29 20:01:36 2000



From: Richard R. Vanfleet :      vanfleet-at-physics.ucf.edu
Date: Tue, 29 Aug 2000 20:53:24 -0400
Subject: Hitachi HD-2000 comments

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At the recent Microscopy and Microanalysis meeting in Philadelphia, I gave
a talk entitled "The Hitachi HD-2000 In Semiconductor Manufacturing Support
And Research". Based on the feed back and comments I have received, I
would like to restate the goals of my talk and my opinions for all that are
interested.

The HD-2000 was designed as a tool for failure mode analysis and
manufacturing support. It does a wonderful job in this regard. It
combines traditional SEM imaging with basic STEM operation giving SEM and
TEM type capabilities in the same package. Holders are compatible with the
Hitachi FIB and the rapid exchange between these tools increases the ease
and success rate for manufacturing support. Use is very straightforward
and requires very little training for successful and productive use. The
Cirent Semiconductor/Lucent technologies primary users are extremely happy
with their microscope and it has increased their capabilities in support of
the semiconductor fabrication lines.

Because of its dedicated STEM design and cold field emission gun, it has
potential to be a successful research level microscope for imaging and
spectroscopy. In my opinion, the microscope as shipped and installed at
the Lucent manufacturing site in Orlando has some key shortcomings that
will inhibit its implementation at this level. However, Hitachi is
currently developing improved diffraction capabilities and EELS options
that should address my concerns. I look forward to seeing and evaluating
their improvements.

I would be glad to discuss my opinions and impressions with anyone interested.

Richard Vanfleet
vanfleet-at-physics.ucf.edu
407-823-2600

Disclaimer: I am not employed by or affiliated in any way with Hitachi.




From daemon Tue Aug 29 23:17:36 2000



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Wed, 30 Aug 2000 16:11:25 GMT+1200
Subject: Re: Link AN10000 problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi, Mick

Have you changed anything around the eds setup?
Plugged a new desk lamp into a mains outlet nearby?
Changed the layout of the cabling?
What you describe is a pretty big jump, but it may pay to check the
simple things before calling in the man.

I really messed up my PC-based system a while back by connecting up
an external CD writer whose mains lead went to a socket that was on a
different supply circuit, thereby producing a nice big
noise-capturing earth loop.

cheers

rtch





} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Fellow microscopists,
}
} We are still using a 15 year old Link AN10000 x-ray detector. The widths
} of the peaks have suddenly become much wider. The zero beam or strobe used
} to be around 80 eV FWHM, and now is around 360 eV, with no beam in the
} column. To the best of my knowledge there is no light in the column, and
} the dewar has plenty of LN2 in it, so I am assuming the crystal is
} cold. Also, if I disconnect the Lemo connector (carrying the signal from
} the detector) the FWHM drops to about 20 eV FWHM. If anyone has any
} suggestions I would be very appreciative.
}
} Thanks,
}
} Mick Thomas
} -------------------------------------------------
} Mick Thomas
} UHV-STEM Laboratory
} E-1 Clark Hall
} Cornell University
} Ithaca, NY 14853
}
} Phone: 607-255-0650
} Fax: 607-255-7658
} e-mail: mgt3-at-ccmr.cornell.edu
}
}
Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand



From daemon Wed Aug 30 06:01:26 2000



From: Keith Ryan :      kpr-at-ccms.ac.uk
Date: Wed, 30 Aug 2000 11:50:24 +0100
Subject: SEM - mucus removal from specimens

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greetings from Plymouth UK

How can we remove mucus from fresh / fixed tissue specimens -
specifically fish / squid? A student has been recommended to try
s-carboxy-methyl-L-cysteine but can find no information as to how to
do this, e.g. concentration, time, temperature. Any information would
be welcome about this or any other methods.

Thanks - Keith Ryan


_______________________
Keith Ryan (Dr)
Marine Biological Association
Citadel Hill
Plymouth
Devon PL1 2PB
England

Tel. 0044 (0)1752 633279 (with answer machine)
also 0044 (0)1752 633249
Fax. 0044 (0)1752 633102

e-mail: kpr-at-wpo.nerc.ac.uk



From daemon Wed Aug 30 09:42:44 2000



From: LAB. DE MICROSCOPIA ELECTRONICA - FI - UNER :      microsc-at-fi.uner.edu.ar
Date: Wed, 30 Aug 2000 11:31:22 +0000
Subject: Looking for accesories to Philips EM201

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I wish to obtain the accesories for a TEM Philips EM201 listed bellow

Plate numbering device PW 6543/00
Automated exposure system PW 6542..

the parts can be used...

any help is welcome...

thanks in advance

Fernando
===================================================
Fernando D. Balducci
Laboratorio de Microscopia Electrónica
Facultad de Ingeniería - Bioingenieria
Universidad Nacional de Entre Rios
Argentina
e-mail: microsc-at-fi.uner.edu.ar
tel: 54 43 975100
fax: 54 43 975077


===================================================



From daemon Wed Aug 30 10:20:28 2000



From: Sara Miller :      saram-at-duke.edu
Date: Wed, 30 Aug 2000 11:03:57 -0400 (EDT)
Subject: Re: SEM - mucus removal from specimens

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On Wed, 30 Aug 2000, Keith Ryan wrote:

} Date: Wed, 30 Aug 2000 11:50:24 +0100
} From: Keith Ryan {kpr-at-ccms.ac.uk}
} To: Microscopy-at-sparc5.microscopy.com
} Subject: SEM - mucus removal from specimens
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Greetings from Plymouth UK
}
} How can we remove mucus from fresh / fixed tissue specimens -
} specifically fish / squid? A student has been recommended to try
} s-carboxy-methyl-L-cysteine but can find no information as to how to
} do this, e.g. concentration, time, temperature. Any information would
} be welcome about this or any other methods.
}
} Thanks - Keith Ryan
}
}
} _______________________
} Keith Ryan (Dr)
} Marine Biological Association
} Citadel Hill
} Plymouth
} Devon PL1 2PB
} England
}
} Tel. 0044 (0)1752 633279 (with answer machine)
} also 0044 (0)1752 633249
} Fax. 0044 (0)1752 633102
}
} e-mail: kpr-at-wpo.nerc.ac.uk

We use Sputolysin to dissolve mucus in human lung washes. You can make
it yourself with 0.1 g dithiothreitol in 100 ml water. Keep this as a
stock. When ready for use, dilute 1:10 with water, and use this dilution
1:1 with sample. Let sit at room temp with periodic swirling for 30-60
min. Add more and repeat if necessary. For our procedure, it thins the
mucus so that we can prepare the sample for negative staining to look
for viruses.

NOW, I have no idea what is in fish slime or whether this concoction will
work, but it's easy enough to try. I don't know about the cysteine and
will be interested to hear what you find works.

Good luck,
Sara

}
}

Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265




From daemon Wed Aug 30 10:36:50 2000



From: Bob Munn :      rjmunn-at-ucdavis.edu
Date: Wed, 30 Aug 2000 08:34:48 -0700
Subject: Removing mucus

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Keith Ryan - I can relay a little tip about this but I have not studied the
chemistry in detail. A group here was doing SEM on lung and airway
tissues. They were prepping the tissue for SEM by fixation, dehydration in
ethanol, then using hexamethyldisilazane to dry the samples. They
discovered that mucus was covering the surface of the airways. They simply
added a ten minute step in xylene between the last alcohol and the
HMDS. The mucus disappeared. It might be worth a try. Good luck.
Sincerely,

Robert J. Munn
Electron Microscopy Laboratory
Department of Medical Pathology
University of California, Davis
School of Medicine
Davis, CA 95616

Phone (530) 752-3165
FAX (530) 754-8220
EMail rjmunn-at-ucdavis.edu




From daemon Wed Aug 30 10:47:21 2000



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Wed, 30 Aug 2000 08:37:27 -0700
Subject: Re: Link AN10000 problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Mick,
When I had an increase in peak width in my Kevex last year, I warmed up the
detector to remove any water ice that had accumulated in the bottom of the
dewar or on the crystal. Turn off the bias, short out the bias plug, pour
out the liquid nitrogen, then blow hot air into the dewar until everything
is warm. Unfortunately, although the resolution improved 10 eV per channel,
the dewar now had poor holding time, so I had to have it re-pumped. Now,
everything is great. The other problem can be related to poor grounding in
the pre-amp, but that caused excessive dead-time.
At 04:01 PM 8/29/00 -0700, you wrote:
}
} Fellow microscopists,
}
} We are still using a 15 year old Link AN10000 x-ray detector. The widths
} of the peaks have suddenly become much wider. The zero beam or strobe used
} to be around 80 eV FWHM, and now is around 360 eV, with no beam in the
} column. To the best of my knowledge there is no light in the column, and
} the dewar has plenty of LN2 in it, so I am assuming the crystal is
} cold. Also, if I disconnect the Lemo connector (carrying the signal from
} the detector) the FWHM drops to about 20 eV FWHM. If anyone has any
} suggestions I would be very appreciative.
}
} Thanks,
}
} Mick Thomas
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca




From daemon Wed Aug 30 11:13:14 2000



From: simon watkins :      swatkins+-at-pitt.edu
Date: Wed, 30 Aug 2000 11:55:59 -0400
Subject: removing mucus

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We remove mucus using a product used to clear mucus from airway and bowel in
the clinical environment, it is Acetylcysteine, used at 1% concentration I
believe. The only problem is that if there are other goblet cells in the
tissue they will release immediately leading to yet more mucus.....
It works though
Simon


---------------------------
Simon C. Watkins Ph.D. MRC Path
Associate Professor, Cell Biology and Physiology
Director: Center for Biologic Imaging
BSTS 225
University of Pittsburgh
Pittsburgh PA 15261
Tel:412-648-3051
Fax:412-648-8330
http://sbic6.sbic.pitt.edu
-----------------------------------




From daemon Wed Aug 30 13:19:24 2000



From: David Bell :      dcb-at-x32-135.cie.umn.edu
Date: Wed, 30 Aug 2000 13:07:30 -0500
Subject: Help Needed: Looking at DNA strands with TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Currently we are looking at stands of DNA that have been stained
with Uranyl Acetate and gold labeled, and then deposited on to
Collodion film, which the strands stick to, nicely.

However, at high KeV or focused spot the film breaks easily,
what I would like to do is use a carbon film, which is more robust
but my problem is the DNA will not stick to this.

Perhaps someone who is more expert at DNA imaging could suggest
a way to produce a more robust sample?

Many Thanks in advance

David


--
Dr. David C. Bell (612)-624-1677
AEM Manager, IT Chafac dcb-at-umn.edu
16 Shepherd Labs, University of Minnesota
http://resolution.umn.edu
100 Union St S.E. Minneapolis, MN USA 55455



From daemon Wed Aug 30 14:26:53 2000



From: Tamara Howard :      howard-at-cshl.org
Date: Wed, 30 Aug 2000 15:11:32 -0400 (EDT)
Subject: Re: Help Needed: Looking at DNA strands with TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Have you tried collodion on carbon? Then you'd have the best of both (put
the carbon on the collodion, then use the "back" of the grid). Or try some
substrate other than collodion that is more stable in the beam - Butvar,
for example.

However, doesn't the DNA "community" usually use carbon films? I've done
DNA on carbon - just glow-disharge the carbon film within a few hours
before you put the DNA on. It is more annoying than using straight plastic
films, but more stable once you get to the 'scope.

Tamara Howard
CSHL

On Wed, 30 Aug 2000, David Bell wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} Currently we are looking at stands of DNA that have been stained
} with Uranyl Acetate and gold labeled, and then deposited on to
} Collodion film, which the strands stick to, nicely.
}
} However, at high KeV or focused spot the film breaks easily,
} what I would like to do is use a carbon film, which is more robust
} but my problem is the DNA will not stick to this.
}
} Perhaps someone who is more expert at DNA imaging could suggest
} a way to produce a more robust sample?
}
} Many Thanks in advance
}
} David
}
}
} --
} Dr. David C. Bell (612)-624-1677
} AEM Manager, IT Chafac dcb-at-umn.edu
} 16 Shepherd Labs, University of Minnesota
} http://resolution.umn.edu
} 100 Union St S.E. Minneapolis, MN USA 55455
}
}




From daemon Wed Aug 30 15:13:47 2000



From: tellis2-at-hallmark.com
Date: Wed, 30 Aug 2000 15:04:23 -0500
Subject: mineralogy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have been asked to identify a figurine said to be "chalkware" has anyone
seen or know a definition of that term? Is there a legal definition of
chalkware? Would there be a problem calling a plastic filled with 20-30%
calcium carbonate - chalkware?
The sample was made of gypsum (calcium sulfate) core with a calcium
carbonate (whitewash) then painted which makes it what is called
carnival-ware.
Thanks for any help.
Terry Ellis




From daemon Wed Aug 30 16:33:52 2000



From: Sara Miller :      saram-at-duke.edu
Date: Wed, 30 Aug 2000 17:16:55 -0400 (EDT)
Subject: Re: Help Needed: Looking at DNA strands with TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


You could try to evaporate a light coat of carbon over the whole grid
after the DNA is stuck and stained. You could also try Formvar which is
a little sturdier than collodion.

On Wed, 30 Aug 2000, David Bell wrote:

} Date: Wed, 30 Aug 2000 13:07:30 -0500
} From: David Bell {dcb-at-x32-135.cie.umn.edu}
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Help Needed: Looking at DNA strands with TEM
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} Currently we are looking at stands of DNA that have been stained
} with Uranyl Acetate and gold labeled, and then deposited on to
} Collodion film, which the strands stick to, nicely.
}
} However, at high KeV or focused spot the film breaks easily,
} what I would like to do is use a carbon film, which is more robust
} but my problem is the DNA will not stick to this.
}
} Perhaps someone who is more expert at DNA imaging could suggest
} a way to produce a more robust sample?
}
} Many Thanks in advance
}
} David
}
}
} --
} Dr. David C. Bell (612)-624-1677
} AEM Manager, IT Chafac dcb-at-umn.edu
} 16 Shepherd Labs, University of Minnesota
} http://resolution.umn.edu
} 100 Union St S.E. Minneapolis, MN USA 55455
}
}

Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265




From daemon Wed Aug 30 16:47:23 2000



From: Milo Kral :      m.kral-at-mech.canterbury.ac.nz
Date: Thu, 31 Aug 2000 09:36:15 +1200
Subject: Help with TEM of Mg alloy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am having serious problems preparing thin foils of a Mg-2.5Ag-2Nd
alloy using a Fischione twin jet. I have tried two electrolytes: 5%
perchloric in ethanol; 5.3g lithium chloride, 11g magnesium
perchlorate, 500ml methanol and 100ml 2-butoxy-ethanol. The main
problem I have seems to occur on removal from the Fischione fixture,
the specimen continues to react the even the slightest amount of
electrolyte-no matter what efforts I take to dilute it. Then we are
left with no thin area and lots of corrosion product on the remaining
thick foil.

Any advice will be greatly appreciated!

Thanks
Milo



From daemon Wed Aug 30 18:08:22 2000



From: David Henriks :      Henriks-at-CompuServe.COM
Date: Wed, 30 Aug 2000 18:57:25 -0400
Subject: Help with TEM of Mg alloy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Milo:

While I haven't used the Fischione polisher, we do manufacture our own
electropolisher so I have some experience to share. As our Model 550 is
also capable of being used for straight chemical etching (as with HF and
Bromine methanol solutions for semiconductor applications) we developed a
rinser that will rinse the sample automatically immediately after the
perforation is detected. The problem you have is that once a perforation
is detected, even the slightest additional etching will quickly etch away
the thin area surrounding the hole. If you are doing a purely electrolytic
etch, then etching should stop as soon as the power is shut off. Perhaps
you are also achieving some chemical etch which will continue until all of
the etchant is washed away. A few things you may want to try are:

1) See if there is anyway you can rig up an automatic rinse system to the
polisher. This would be ideal, but I do not know how practical it would
be.

2) Try to simplify the sample holder so that electrolyte does not get
trapped within the holder. If you are experiencing etching after the power
is shut off, then any chemicals trapped within the holder will leak out and
cause the sample to etch further. Our single jet system places the
specimen on a pedestal where the electrolyte cannot become trapped. The
sample is not submerged in the electrolyte so the electrolyte quickly
washes away. This is also what makes our rinsing attachment easy to
implement.

3) If etching still continues after removing the fixture, then you may want
to try to adjust the distance between the jets and the sample. With our
Model 550 you can change the shape of the dimple in the sample by moving
the jet nozzle up or down. Placing the nozzle closer to the sample will
provide a larger thin are and will give you additional time to stop the
chemical etching before all of the thin area is etched away.

4) With our system you can easily change the LED used in the detection
circuit to select a wavelength that is compatible with your material. We
have been able to automatically shut off the system in some cases just
prior to perforation. If you are able to do this with your system, perhaps
the additional etching that occurs as you remove the sample holder will
produce a sample with the appropriate thin area.

Of course, my first suggestion would be to buy one of my jet polishers! 8-)

I hope this helps to some degree. If I can be of any additional
assistance, please feel free to contact me.

DISCLAIMER: South Bay Technology produces equipment and supplies as
described above and, therefore, has a vested interest in promoting their
use.

Best regards-

David
Writing at 3:33:38 PM on 08/30/2000

***************************************************************************
************************

David Henriks
Vice President TEL: 800-728-2233
(toll free in the USA)
South Bay Technology, Inc. +1-949-492-2600
1120 Via Callejon FAX: +1-949-492-1499
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

***************************************************************************
************************

} } } } } Please visit us at http://www.southbaytech.com { { { { {

Manufacturers of precision sample preparation equipment and supplies for
metallography, crystallography and electron microscopy.
Message text written by Milo Kral
}
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


I am having serious problems preparing thin foils of a Mg-2.5Ag-2Nd
alloy using a Fischione twin jet. I have tried two electrolytes: 5%
perchloric in ethanol; 5.3g lithium chloride, 11g magnesium
perchlorate, 500ml methanol and 100ml 2-butoxy-ethanol. The main
problem I have seems to occur on removal from the Fischione fixture,
the specimen continues to react the even the slightest amount of
electrolyte-no matter what efforts I take to dilute it. Then we are
left with no thin area and lots of corrosion product on the remaining
thick foil.

Any advice will be greatly appreciated!

Thanks
Milo


{



From daemon Wed Aug 30 22:22:40 2000



From: Marek Malecki :      mmm-at-ucsd.edu
Date: Wed, 30 Aug 2000 20:08:57 -0700
Subject: Re: Help Needed: Looking at DNA strands with TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


David,
You can stabilize your collodion films with DNA on them through thermal or
eb evaporation of carbon layer prior to TEM.
Alternatively, you can modify carbon films through alkylamine treatment
prior to spreading.
Marek.


}
} }
} } Currently we are looking at stands of DNA that have been stained
} } with Uranyl Acetate and gold labeled, and then deposited on to
} } Collodion film, which the strands stick to, nicely.
} }
} } However, at high KeV or focused spot the film breaks easily,
} } what I would like to do is use a carbon film, which is more robust
} } but my problem is the DNA will not stick to this.
} }
} } Perhaps someone who is more expert at DNA imaging could suggest
} } a way to produce a more robust sample?
} }
} } Many Thanks in advance
} }
} } David
} }
} }
} } --
} } Dr. David C. Bell (612)-624-1677
} } AEM Manager, IT Chafac dcb-at-umn.edu
} } 16 Shepherd Labs, University of Minnesota
} } http://resolution.umn.edu
} } 100 Union St S.E. Minneapolis, MN USA 55455
} }
} }
}


Marek Malecki, M.D., Ph.D.
Director and Principal Investigator

Molecular Imaging Laboratories
University of California at San Diego
address: 1500 Suite Bonner Hall, 9500 Gilman Drive, La Jolla, CA 92093-0368
phone: 8588223373
fax: 8588223715
pager: 8586161420
e.mail: mmm-at-ucsd.edu
e.pager: 1620024619-at-alphapage.airtouch.com
www site: http://mil.ucsd.edu/
ftp site: mil1.ucsd.edu








From daemon Thu Aug 31 01:09:05 2000



From: Colin Reid :      creid-at-tcd.ie
Date: Thu, 31 Aug 2000 07:07:29 +0100
Subject: Re: Link AN10000 problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hi,

I am not sure if warming will cure the problem, but is certainly worth
trying. As I recall a lot of the AN10,000's had heater circuits built into
the detector, with a button on the pulse processor. This may have only
been the turret detectors. If present it will be less destructive than hot
water.

Best wishes,

Colin

Colin Reid,
Electron Microscope Unit,
Trinity College Dublin,
Dublin 2.

Tel: 353-1-6081820
Fax: 353-1-6770438
Email: creid-at-tcd.ie
Home Page: http://www.tcd.ie/Electron_Microscope/emu/home.htm


-----Original Message-----
} From: Mary Mager [mailto:mager-at-interchange.ubc.ca]
Sent: Wednesday, August 30, 2000 4:37 PM
To: Mick Thomas
Cc: Microscopy-at-sparc5.microscopy.com


Dear Mick,
When I had an increase in peak width in my Kevex last year, I warmed up the
detector to remove any water ice that had accumulated in the bottom of the
dewar or on the crystal. Turn off the bias, short out the bias plug, pour
out the liquid nitrogen, then blow hot air into the dewar until everything
is warm. Unfortunately, although the resolution improved 10 eV per channel,
the dewar now had poor holding time, so I had to have it re-pumped. Now,
everything is great. The other problem can be related to poor grounding in
the pre-amp, but that caused excessive dead-time.
At 04:01 PM 8/29/00 -0700, you wrote:
}
} Fellow microscopists,
}
} We are still using a 15 year old Link AN10000 x-ray detector. The widths
} of the peaks have suddenly become much wider. The zero beam or strobe used
} to be around 80 eV FWHM, and now is around 360 eV, with no beam in the
} column. To the best of my knowledge there is no light in the column, and
} the dewar has plenty of LN2 in it, so I am assuming the crystal is
} cold. Also, if I disconnect the Lemo connector (carrying the signal from
} the detector) the FWHM drops to about 20 eV FWHM. If anyone has any
} suggestions I would be very appreciative.
}
} Thanks,
}
} Mick Thomas
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca





From daemon Thu Aug 31 03:04:18 2000



From: PETER HEIMANN :      peter.heimann-at-biologie.uni-bielefeld.de
Date: Thu, 31 Aug 2000 09:49:50 +0200
Subject: Re: Help Needed: Looking at DNA strands with TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


probably the method with the most consistent results (and easy to
perform; students do it in our EM-course) is the method by Lang
and Mitani (1969). in short, put your cytochrome C stabilized DNA
on the collodion film (without carbon) fix with uranylacetate/METOH
and perform low angle (approx. 5 °) platinum rotation shadowing (2-
3 nanometer) followed by stationary, vertical (90°) carbon
shadowing. specimens are virtually undestroyable and last "forever".

good luck!
peter

**************************************************
please reply to:

peter.heimann-at-biologie.uni-bielefeld.de ("E-MAIL")

Dr. Peter Heimann
Developmental Biology; W7-107
University of Bielefeld
D 33501 Bielefeld / Germany
phone: (0521) 106 - 5628 / 5627
FAX : (0521) 106 - 5654
WEB-Site: http://www.uni-bielefeld.de/biologie/Entwicklungsbiologie
WEB-Site: http://www.uni-bielefeld.de/SFB549
******************************************************



From daemon Thu Aug 31 07:57:30 2000



From: Tom Phillips :      PhillipsT-at-missouri.edu
Date: Thu, 31 Aug 2000 07:44:19 -0500
Subject: Re: removing mucus

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I knew acetylcysteine has been used for airway and stomach mucus but
we never had much luck with it with intestinal mucus (rat intestines
or human goblet cells cultures). Simon says "bowel" so maybe he
means stomach or perhaps he has better luck with intestinal mucus
also. I am not an expert with acetylcysteine so I will defer to him
on that point. But I can easily believe him when he says that it
causes increased mucus secretion on fresh tissue. We have found that
most, if not all, of the treatments to remove or "thin" mucus are
good only for biochemical preps or fixed tissues since it is
especially easy to trigger a goblet cell. Even vigorous washing is
enough to set them off.




} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

--
Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)





From daemon Thu Aug 31 08:13:17 2000



From: Ni, Chao-Ying :      CYNi-at-rodel.com
Date: Thu, 31 Aug 2000 09:01:56 -0400
Subject: TEM: Melting and Flow of Nanoparticles During TEM Observation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


David,

The temperature increase in your sample is not only related to the keV and
e-dose you use, but also related to the thermal conductivity of your sample,
it thickness, surface condition, and its surrounding materials. So I would
think it is almost impossible in your case to measure experimentally or to
estimate theoretically the temperature increase.

The reason for melting point decrease of any kind of nona-particles is
obvious because of the tremendous surface to volume ratio. The surface Gibbs
free energy should be much higher in nano-systems, and hence such systems
are relatively unstable. That's why most nano-particles have to be kept in
aqueous suspension and with additives.

To avoid the problem, try the following:

1. Spot size: 2 or 3 (When I drive TEM, I always first try relatively
smaller spot size, usually at 2. Actually I hardly have the need to use spot
size 1 on a 200kV scope. The materials I checked range from metals,
intermetallic compounds, ceramics, semiconductors and polymers, either thin
film or bulk or nano particles.)
2. Different keV, either higher or lower keV could be beneficial to
decreasing the damage to a sample depending on which specific heating
mechanism dominates.
3. Use a smaller condenser aperture.
4. Coat a very thin C film on your grids before observation.

Have a nice day!

Chao-Ying Ni
Rodel Inc.
451 Bellevue Road
Newark, DE 19713
(302) 366-0500 ext 2812


-----Original Message-----
} From: David R Hull [mailto:David.R.Hull-at-grc.nasa.gov]
Sent: Tuesday, August 29, 2000 1:22 PM
To: 'Microscopy Listserver'


We have been looking at the use of various catalysts for production
carbon nanotubes. Recently we had a sample that behaved in a very
unusual way. Upon focusing the electron beam on a cluster of
nanotubes, the catalyst particles became fluid and ran through the
tubes at about 50-100 nm/sec. I was a little surprised by the
movement. The catalyst particles would stop moving within 2-5
seconds.

I have found one reference, Buffat and Borel, Phys. Rev A13,
2287(1976), that showed the melting temperature of gold nanoparticles
could be reduced by 600C! for nanoparticles less than 5 nm.

In our case we have cobalt nanoparticles less than 10 nm.

My question is how high a temperature might we expect to heat the
sample in the electron beam with the following parameters;

200 kV, LaB6, 20-30 microamp emmission current, spot size 1, 150
micron condenser aperture.

Have others seen this phenomenon?
David R. Hull
NASA Glenn Research Center at Lewis Field
Advanced Metallics Branch
Mail Stop 49-1
21000 Brookpark Road
Cleveland, OH 44135

(216) 433-3281
fax (216)977- 7132
david.r.hull-at-grc.nasa.gov



From daemon Thu Aug 31 09:12:46 2000



From: Richard R. Vanfleet :      vanfleet-at-physics.ucf.edu
Date: Thu, 31 Aug 2000 09:58:16 -0400
Subject: Re: TEM: Melting and Flow of Nanoparticles During TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


David,

The short answer is, that if conditions are right you can get
significant local heating under the beam. I (and others before me) have
used this technique to "melt" metal chloride crystallites and form pure
metal nanoparticles inside the microscope. The key issues are how much
energy you are depositing in the specimen (thickness and composition of
specimen are important here) and how fast the specimen can carry the heat
away. An area of the specimen that does not have good thermal contact to
the grid can become very hot. I would suggest looking at Reimer
"Transmission Electron Microscopy" starting about page 431 with a section
called Specimen Heating.

I have tried some of these calculations before so if you run into problems
I might be able to help.

Richard



At 01:21 PM 8/29/00 -0400, David R Hull wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Richard Vanfleet
Assistant Professor
Advanced Materials Processing and Analysis Center (AMPAC)
and Department of Physics

Mail:
Department of Physics
University of Central Florida
4000 Central Florida Blvd.
Orlando, FL 32816-2385

Office: 305B MAP
Phone: (407)823-2600
FAX: (407)823-5112
vanfleet-at-physics.ucf.edu




From daemon Thu Aug 31 09:12:53 2000



From: Philippe-Andre Buffat :      philippe.buffat-at-epfl.ch
Date: Thu, 31 Aug 2000 16:02:54 +0200
Subject: Fwd: TEM: Melting and Flow of Nanoparticles During TEM Observation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,

David R. Hull reported that catalyst particles became fluid in
nanotubes under TEM examinationŠ and asked for temperature rise.

I guess that there is some lowering of the melting temperature for
10nm cobalt particles, although I do not have right now the data to
compute it (in particular the interfacial energy Co-nanotube).

But about temperature rise, you have a very nice situation where
inelastic interaction in the nanotubes cluster may lead to a
relatively strong energy absorption, while the thermal dissipation by
conductivity is low. There is only thermal radiation left for energy
dissipation at the surface of the cluster and this may lead to
temperature rise of several hundreds of C degrees.

We did a nice experiment (unpublished) 20 years ago with clusters a
few microns in diameter containing Ag nanoparticles lying on a carbon
film/copper grid. When the electron beam was focused on a cluster
(about same diameter, current for "normal observation"), the cluster
was instantaneously vaporized and we can follow nucleation, growth
and sintering of Ag particles from nm to 50nm running on the film in
the same or the next grid opening.

Considering the silver vapour pressure with temperature, this proves
that the temperature was by far above 1000 K, more probably toward
1500 K. But again this strange reaction is due to

i) the size of the cluster (significant energy/electron absorption)
ii) the low thermal dissipation by conductivity in the film compared
to energy absorption
iii) the low surface to volume ratio (= low energy dissipation by
radiation / energy absorption) in these "large" clusters

It should be pointed out that for isolated nanocrystals, the
situation is opposite, the surface to volume is much higher, as the
radiation dissipation to energy absorption. In addition the thermal
conductivity of the film becomes relatively more important. The
(phonon) temperature rise is now only of a few C or tens of C even
under strong irradiation.

Yours

Philippe Buffat



} Delivered-To: philippe.buffat-at-epfl.ch
} X-Sender: mshull-at-popserve.grc.nasa.gov
} Date: Tue, 29 Aug 2000 13:21:37 -0400
} To: "'Microscopy Listserver'" {Microscopy-at-sparc5.microscopy.com}
} From: David R Hull {David.R.Hull-at-grc.nasa.gov}
} Subject: TEM: Melting and Flow of Nanoparticles During TEM Observation
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} We have been looking at the use of various catalysts for production
} carbon nanotubes. Recently we had a sample that behaved in a very
} unusual way. Upon focusing the electron beam on a cluster of
} nanotubes, the catalyst particles became fluid and ran through the
} tubes at about 50-100 nm/sec. I was a little surprised by the
} movement. The catalyst particles would stop moving within 2-5
} seconds.
}
} I have found one reference, Buffat and Borel, Phys. Rev A13,
} 2287(1976), that showed the melting temperature of gold
} nanoparticles could be reduced by 600C! for nanoparticles less than
} 5 nm.
}
} In our case we have cobalt nanoparticles less than 10 nm.
}
} My question is how high a temperature might we expect to heat the
} sample in the electron beam with the following parameters;
}
} 200 kV, LaB6, 20-30 microamp emmission current, spot size 1, 150
} micron condenser aperture.
}
} Have others seen this phenomenon?
} David R. Hull
} NASA Glenn Research Center at Lewis Field
} Advanced Metallics Branch
} Mail Stop 49-1
} 21000 Brookpark Road
} Cleveland, OH 44135
}
} (216) 433-3281
} fax (216)977- 7132
} david.r.hull-at-grc.nasa.gov

__________________________________________________________________
Philippe Buffat
Ecole Polytechnique Federale de Lausanne (EPFL)
Centre Interdepartemental de Microscopie Electronique
Address: EPFL-CIME, Batiment MX-C, CH-1015 Lausanne, Switzerland
Phone: +41(21)693 29 83 Fax: +41(21)693 44 01 (Central European Time)
E-mail: philippe.buffat-at-epfl.ch, WWW URL http://cimewww.epfl.ch/
______________________________ Eudora F2.1 ___________________________



From daemon Thu Aug 31 09:33:47 2000



From: Karen Kelley :      klv-at-biotech.ufl.edu
Date: Wed, 30 Aug 2000 22:24:38 -0400
Subject: albumin microspheres

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have a sample of albumin microspheres that need to be embedded and
sectioned for TEM. They range from 2-30µm in size. Does anyone have
experience embedding such a sample? If I embed them straight into a resin
then they may pull out of the resin during sectioning. Any ideas?
Karen Kelley
Senior Electron Microscopist
UF Biotechnology Electron Microscopy Core Lab
Box 118525 Gainesville Florida 32611
lab:352-392-1184 fax: 352-846-0251
email: klv-at-biotech.ufl.edu
http://www.biotech.ufl.edu/~emcl/staff/karenpage.html



From daemon Thu Aug 31 09:49:05 2000



From: Michael B. Ferrari :      ferrari-at-uark.edu
Date: Thu, 31 Aug 2000 09:40:05 +0800
Subject: fixation/probes for yolk

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All,

We're going to be fixing and staining Xenopus cells to examine their
yolk platelets, and I was hoping someone has had good experience with lipid
fixation and suitable fluorescent lipophilic probes for this system.
Specifically, we don't want any of our yolk platelets to float away during
fixation and membrane permeabilization. I'm also curious as to which Di"X"
analogs and/or Bodipy's have worked best for vitellogenin and phosvitin
containing yolk. Thanks in advance.

Mike



Michael B. Ferrari

Department of Biological Sciences
University of Arkansas
Fayetteville, Arkansas 72701
Ph: 501-575-6372
Fax: 501-575-5349
email: ferrari-at-uark.edu
http://biology.uark.edu/ferrari





From daemon Thu Aug 31 10:45:55 2000



From: Marie E. Cantino :      cantino-at-uconnvm.uconn.edu
Date: Thu, 31 Aug 2000 11:35:46 -0400
Subject: Re: albumin microspheres

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Karen,

A student working in my lab made ultrathin sections of polymer coated
albumin microspheres either lyophilized and embedded in acrylic resin, or
dehydrated from an aqueous solution and embedded in epoxy resin. I don't
remember any problem with pullout, and she got reasonable looking
micrographs in both preps.

Marie

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Dr. Marie E. Cantino
Dept. of Physiology and Neurobiology, U-2131
University of Connecticut
Storrs, CT 06269-2131
Phone: 860-486-3588
Fax: 860-486-1936





From daemon Thu Aug 31 11:08:37 2000



From: werner-at-rosharon.wireline.slb.com (Andrew Werner)
Date: Thu, 31 Aug 2000 10:57:20 -0500
Subject: LM - lens cleaning

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


A Leitz Metallux II microscope, which had been stored without air
conditioning for some time, recently came into my care. The objectives and
other internal lenses are actually obscured by corrosion product. Before
anyone gives me a hard time for letting the instrument get into this
condition, let me make it quite clear that I am not the one who put it in
storage, nor did I know about it until recently. I am looking for advice
on how to salvage it.

I removed the objectives from the turret and disassembled one of them as
far as I could - just removed the ring holding the lens in the
housing. The lens is still in place but the dirt / dried corrosion is
plainly exposed. I am reluctant to physically touch the glass for fear of
scratching it. Flushing it with a solvent - water? - seems an appropriate
first step, but I do not know what liquid to try.

Please excuse me if this is the wrong forum in which to post this
query. Any suggestions are welcome. If this instrument appeared on the
capital equipment manifest of any department, I could get a P.O. to get it
repaired - but it does not. I'm the one who has to fix it if it is going
to get fixed. Thanks in advance.

Regards,
Andrew T. Werner
Shaped Charge Research - Metallurgy Laboratory
Schlumberger Reservoir Completions Technology Center
14910 Airline Road, Rosharon, TX 77583-1590
Voice (281) 285-5272 Fax (281) 285-5273




From daemon Thu Aug 31 12:50:44 2000



From: L. D. Marks :      ldm-at-risc4.numis.nwu.edu
Date: Thu, 31 Aug 2000 12:49:54 -0500 (CDT)
Subject: RE: Melting and Flow of Nanoparticles During TEM Observation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I think one important point needs to be made - are you sure
the particles are molten? When you do a lot of TEM, you tend
to forget the size and relevant time scales. A typical phonon
period is about 10-10 seconds, which is similar in magnitude
to the typical attempt time for single-atom diffusion. Hence
it is sometimes surprising that samples sit still for long
enough for one to take an image! In many cases, particularly
at a free surface, what one is observing is a time average of
thousands to millions of single-atom diffusion events.

Consequently what appears to be liquid-like behavior may very
well be reasonably rapid solid-state diffusion. You do not
need to invoke high temperatures in order to explain apparently
rapid (in the microscope) motion of nanoparticles. There may
be some heating (although direct displacement via radiolytic
or knockon processes will be more efficient than heat), but
one does not need more than a modest rise to explain the
observation in most cases.

++++++++++++++++++++++++++++++++++++++++++++++++
Laurence Marks
Department of Materials Science and Engineering
Northwestern University
Tel: (847) 491-3996 Fax: (847) 491-7820
mailto:ldm-at-risc4.numis.nwu.edu
http://www.numis.nwu.edu
++++++++++++++++++++++++++++++++++++++++++++++++





From daemon Thu Aug 31 12:56:41 2000



From: Buckingham, Steve :      sbuckingham-at-excellatron.com
Date: Thu, 31 Aug 2000 13:47:16 -0400
Subject: Microscopist position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all,
I'm trying to put together a proposal to hire someone to run our JSM
840, but I'm not really sure of the salary scale for this kind of position.
I would appreciate it if anyone could suggest a range or offer advice on the
subject preferably to my personal e-mail at sbucks-at-charter.net Any
suggestions would be appreciated.
Many Thanks,

Steve Buckingham
770 438 2201




From daemon Thu Aug 31 13:48:51 2000



From: Chow, David :      David.Chow-at-nrc.ca
Date: Thu, 31 Aug 2000 14:38:18 -0400
Subject: RE: Microscopist position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Steve:

You should have no problems finding someone for a starting salary of
approximately $60,000.

David Chow
E-mail: david.chow-at-nrc.ca {mailto:david.chow-at-nrc.ca}


-----Original Message-----
From: Buckingham, Steve
[mailto:sbuckingham-at-excellatron.com]
Sent: Thursday, August 31, 2000 1:47 PM
To: 'Microscopy-at-MSA.Microscopy.Com'
Subject: Microscopist position


------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society
of America
To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html

-----------------------------------------------------------------------.


Hi all,
I'm trying to put together a proposal to hire
someone to run our JSM
840, but I'm not really sure of the salary scale for this
kind of position.
I would appreciate it if anyone could suggest a range or
offer advice on the
subject preferably to my personal e-mail at
sbucks-at-charter.net Any
suggestions would be appreciated.
Many Thanks,

Steve Buckingham
770 438 2201




From daemon Thu Aug 31 14:06:28 2000



From: Philip Oshel :      peoshel-at-facstaff.wisc.edu
Date: Thu, 31 Aug 2000 14:01:19 -0500
Subject: Linux

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Is anyone writing imaging programs for the Linux OS? Or a port of
NIH-Image for Linux?

Phil
--
}}}}}}}}}}}}}}}}}{{{{{{{{{{{{{{{{{
Philip Oshel
Supervisor, AMFSC and BBPIC
Dept. of Animal Health and Biomedical Sciences
University of Wisconsin
1656 Linden Drive
Madison, WI 53706-1581
voice: (608) 263-4162
fax: (608) 262-7420 (dept. fax)



From daemon Thu Aug 31 14:47:42 2000



From: simon watkins :      swatkins+-at-pitt.edu
Date: Thu, 31 Aug 2000 14:24:42 -0400
Subject: Re: removing mucus

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



The tissue we were using were duodenal segments mounted in an ussing
chamber, which were washed and then treated with various strains of bugs to
look at mechanisms of translocation.
It did get rid of the mucus, but I agree with Tom its tough to stop it
coming back!
Simon

-----Original Message-----
} From: Tom Phillips [mailto:PhillipsT-at-missouri.edu]
Sent: Thursday, August 31, 2000 8:44 AM
To: Microscopy-at-sparc5.microscopy.com


I knew acetylcysteine has been used for airway and stomach mucus but
we never had much luck with it with intestinal mucus (rat intestines
or human goblet cells cultures). Simon says "bowel" so maybe he
means stomach or perhaps he has better luck with intestinal mucus
also. I am not an expert with acetylcysteine so I will defer to him
on that point. But I can easily believe him when he says that it
causes increased mucus secretion on fresh tissue. We have found that
most, if not all, of the treatments to remove or "thin" mucus are
good only for biochemical preps or fixed tissues since it is
especially easy to trigger a goblet cell. Even vigorous washing is
enough to set them off.




} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

--
Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)







From daemon Thu Aug 31 15:10:55 2000



From: Kirk Czymmek :      kirk-at-udel.edu
Date: Thu, 31 Aug 2000 15:59:59 -0400
Subject: EM job opening

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Electron Microscopy Laboratory Coordinator


The Department of Material Science and Engineering at the University of
Delaware has an immediate opening for a lab coordinator/instrument scientist
to administer and help further develop the expanding electron microscopy lab
in the college of engineering. The successful candidate should have an
MS/PhD in MSE or related field and extensive EM laboratory experience. The
primary responsibilities of the position include general maintenance of
transmission and scanning microscopes (JEOL 2010FEG and 2000 FX TEM, JEOL
840 SEM) and their ancillary sample preparation equipment, user training,
facility scheduling, and input on future facility development and instrument
acquisition. In addition, it is anticipated that the candidate may pursue
collaborative research with faculty in the college and university. The
salary for the position will be commensurate with successful candidate
qualifications. Applicants should send a cover letter, resume/CV, and list
of 3 references to Professor Darrin J. Pochan, 301 Spencer Lab, Materials
Science and Engineering, University of Delaware, Newark, DE 19716.
Applications will be accepted till September 30, 2000 or until the position
is filled.




From daemon Thu Aug 31 18:01:09 2000



From: David Knecht :      knecht-at-uconn.edu
Date: Thu, 31 Aug 2000 18:42:11 -0400
Subject: Re: LM - lens cleaning

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I often use water first as a cleaner because often it is stuff
soluble in aqueous media that is dripped on the lens and often this
stuff is not soluble in organic solvents. Then I do an ethanol wash
to dry and remove organic soluble stuff.

If I can add to the original question, I have had a great deal of
trouble cleaning lenses and especially CCD imagers due to residual
dust. I find that cotton swabs (at least those I have) leave an
enormous amount of residue behind that cannot be blown off easily.
Does anyone have a source of a more dust free equivalent to the
cotton swab? Dave
--

************************************************************
"Home of the 2000 NCAA Women's Basketball Champions"

Dr. David Knecht
Department of Molecular and Cell Biology
University of Connecticut
75 N. Eagleville Rd. U-125
Storrs, CT 06269-3125
Knecht-at-uconnvm.uconn.edu
860-486-2200 860-486-4331 (fax)
home page: http://www.sp.uconn.edu/~mcbstaff/knecht/knecht.html
************************************************************



From daemon Thu Aug 31 18:41:07 2000



From: William Oliver :      oliver-at-cpt.afip.org
Date: Thu, 31 Aug 2000 18:36:34 -0400 (EDT)
Subject: Re: Linux

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I don't know about NIH-Image, but there are a large number of
imaging programs for Linux. These include:

AVS (see www.avs.com)

Gimp (A Photoshop workalike)

VTK (The Visualization Toolkit, see www.kitware.com)

Image/J is Jave-based and runs on Linux
(see http://rsb.info.nih.gov/ij/)

ImageMagick

ImgStar

There is a Mac Emulator called Executor that
will allow PC users with Linux to run NIH Image.
See www.cs.ubc.ca/spider/ladic/softunix.html

The site above lists a number of IP tools
for UNIX, many of which will run on Linux
boxes.

billo


On Thu, 31 Aug 2000, Philip Oshel wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Is anyone writing imaging programs for the Linux OS? Or a port of
} NIH-Image for Linux?
}
} Phil
} --
} }}}}}}}}}}}}}}}}}{{{{{{{{{{{{{{{{{
} Philip Oshel
} Supervisor, AMFSC and BBPIC
} Dept. of Animal Health and Biomedical Sciences
} University of Wisconsin
} 1656 Linden Drive
} Madison, WI 53706-1581
} voice: (608) 263-4162
} fax: (608) 262-7420 (dept. fax)
}



From daemon Fri Sep 1 03:03:50 2000



From: Gordon Couger :      gcouger-at-couger.com
Date: Fri, 1 Sep 2000 03:30:04 -0500
Subject: Re: LM - lens cleaning

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Phil,

There are some very good packages out there both commercial and GNU. I
will run them down for you.

Gordon
Gordon Couger gcouger-at-couger.com
Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00
----- Original Message -----
} From: "Philip Oshel" {peoshel-at-facstaff.wisc.edu}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Thursday, August 31, 2000 2:01 PM


I have a product from Berkshire 800 242 7000 or 413 528 2602 or 2802 model
LT00e166-R I got from a VCR tech. The are a Camious like swab that don't
seem to leave lent. They do require more pressure on the glass but I have
not had a scratch yet.

I used it to remove a very stubborn water stain on a prism that I couldn't
touch with cotton and anything I had for a solvent. Isopropyl and this
swab made it look easy.

Cotton has a considerable amount of oil on it as grown. Much of it is
removed in processing but it still has some slight oil content that plus
the fact it can pick up a static charge to a point that has to be seen to
be believed make it problematic. If I was trying to use cotton so it
wouldn't stick I would treat it with and anti static solution wash it in a
couple of changes of hexane and use it in a high humidity environment.

A piece of lens tissue works well if you just twist it on a rough stick.\

Gordon
Gordon Couger gcouger-at-couger.com
Stillwater, OK www.couger.com/gcouger

} From: "David Knecht" {knecht-at-uconn.edu}
}
} I often use water first as a cleaner because often it is stuff
} soluble in aqueous media that is dripped on the lens and often this
} stuff is not soluble in organic solvents. Then I do an ethanol wash
} to dry and remove organic soluble stuff.
}
} If I can add to the original question, I have had a great deal of
} trouble cleaning lenses and especially CCD imagers due to residual
} dust. I find that cotton swabs (at least those I have) leave an
} enormous amount of residue behind that cannot be blown off easily.
} Does anyone have a source of a more dust free equivalent to the
} cotton swab? Dave
} --
}

}





From daemon Fri Sep 1 05:02:48 2000



From: Michael Reiner :      Elektronenmikroskopie-at-web.de
Date: Fri, 1 Sep 2000 11:54:22 +0200
Subject: EM: Artifacts in myelin sheaths

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello dear microscopists!

I have a question regarding artifacts in the myelin sheath of a peripheral nerve:
Samples were immersionfixed in Karnovsky´s half-strength fixative(2% Glut, 2% PFA in 0.1M Cacodylate)en bloc stained with osmiumtetroxide and UAc and embedded in Araldite.
The myelin sheaths show the following appearance:
1. The fine lammellar structure is focally disturbed, the layers appear weavy, wobbly ...
2. The outer rings are stained more intense than the inner rings (to the axon).
3. The myelin sheaths are de-rounded, but this seems to be not so grave and I noted this often in other pictures published. Maybe this is "normal"?

I would appreaciate any suggestions from you all to overcome this problem.
Thanks in advance!
Greetings,
Michael


Michael Reiner
University of Cologne, Germany
Dept. of Anatomy I
_______________________________________________________________________
1.000.000 DM gewinnen - kostenlos tippen - http://millionenklick.web.de
IhrName-at-web.de, 8MB Speicher, Verschluesselung - http://freemail.web.de




From daemon Fri Sep 1 07:42:34 2000



From: John Minter :      john.minter-at-kodak.com
Date: Fri, 1 Sep 2000 08:30:03 -0400
Subject: LM: CCD Coverglass cleaning

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


David Knecht asked:
} If I can add to the original question, I have had a great deal of
} trouble cleaning lenses and especially CCD imagers due to residual
} dust. I find that cotton swabs (at least those I have) leave an
} enormous amount of residue behind that cannot be blown off easily.
} Does anyone have a source of a more dust free equivalent to the
} cotton swab?

Kodak has several application notes covering various aspects
of CCD sensors available on our web site:

http://www.kodak.com/US/en/digital/ccd/appNotes.shtml

Specifically, application note DS 00-009 has the recommended procedure for
cleaning the coverglass on our sensors. Of course, we do not speak for
other manufacturers. It is always safest to check with the manufacturer
of your particular sensor for the recommended cleaning procedure.

DS 00-009 is available, in Adobe Acrobat format at:
http://www.kodak.com/US/en/digital/pdf/ccdCoverGlass.pdf


The engineer who cleans our light microscopes tells me that CCD coverglasses
tend to attract dust because of static charges. Good housekeeping practices
and the use of dust covers minimizes the need for cleaning.


Best Regards,

John Minter
Eastman Kodak Company
Analytical Technology Division
Rochester, NY 14650-2152
Phone: (716) 722-3407
FAX: (716) 477-7781
email: john.minter-at-kodak.com




From daemon Fri Sep 1 07:42:35 2000



From: Marie E. Cantino :      cantino-at-uconnvm.uconn.edu
Date: Fri, 1 Sep 2000 08:37:03 -0400
Subject: Re: EM: Artifacts in myelin sheaths

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Michael,

We often see some disturbance of the myelin sheaths. According to a review
chapter by Friedrich and Mugnaini: "The preservation of myelin sheaths
presents a special problem. The membrane lamellae often separate from one
another, even in otherwise well-preserved material. On the other hand, we
have also encountered specimens whose myelin sheaths were reasonably well
preserved, but in which the neuronal elements were poorly fixed.
Apparently the myelin sheaths differ from other tissue components in their
reaction to fixation and they may require special conditions for optimal
preservation. Improvements in the technology of fixation may eventually
eliminate this disparity, but for the present it seems sensible to discount
the condition of myelin sheaths in evaluating fixed material unless the
myelin sheath itself is the main target of the investigation." He also
lists a "Procedure for Myelin"..

This is from a book chapter (unfortunately, the copy I have is missing the
source reference) which may be a number of years old. Perhaps someone else
has some new information on this.

Marie

Dr. Marie E. Cantino
Dept. of Physiology and Neurobiology, U-2131
University of Connecticut
Storrs, CT 06269-2131
Phone: 860-486-3588
Fax: 860-486-1936





From daemon Fri Sep 1 08:13:50 2000



From: Paul Anderson :      paanders-at-lynx.dac.neu.edu
Date: Fri, 1 Sep 2000 09:05:00 -0400 (EDT)
Subject: RE: Melting and Flow of Nanoparticles

Contents Retrieved from Microscopy Listserver Archives
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agreed. in addition, one must be aware of the difference between the
melting point and the temperature of mobility (tamman temperature). the
tamman temp is about 0.52 bulk melting point of a metal in degrees K.
liquid-like behavior of metal particles can occur at this point. the
picture is further complicated by any support interactions. in the case
of nanotubes, you are dealing with a basal plane ({0001} face) of graphite
on which the metal particle is nucleated. all transition metals are
very sensitive to such interfacial phenomenon, which will directly
impact their nucleating/wetting/spreading behavior (see some of
R. T. K. Baker's controlled atmosphere electron microscopy studies,
j. catal.). for instance, evaporated gold will tend to nucleate on
edges of graphite, but not on the basal plane. such behavior was and
still is taken advantage of using gold decoration techniques (pioneered
by g. r. hennig in the 60's). cool stuff!

paul

----------------
Paul E. Anderson
Catalytic and Nanostructured Materials Laboratory
Department of Chemistry
102 Hurtig Hall
Northeastern University
Boston, MA 02115
617 373 5909
FAX 617 373 8795
paanders-at-lynx.neu.edu



From daemon Fri Sep 1 08:36:31 2000



From: George Laing :      scisales-at-ngscorp.com
Date: Fri, 1 Sep 2000 09:31:44 -0700
Subject: RE: LM - lens cleaning

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




} If I can add to the original question, I have had a great deal of
} trouble cleaning lenses and especially CCD imagers due to residual
} dust. I find that cotton swabs (at least those I have) leave an
} enormous amount of residue behind that cannot be blown off easily.
} Does anyone have a source of a more dust free equivalent to the
} cotton swab? Dave


Photographic Solutions makes a product called "Sensor Swab"
http://www.photosol.com/swab.html for use with professional digital
SLR cameras to clean CCD's. They are approved by Kodak for use with
their DCS Camera line. They also make a high quality optics cleaner
called Eclipse.

George Laing
National Graphic Supply







From daemon Fri Sep 1 09:26:42 2000



From: Zhiping LUO :      luo-at-msd.anl.gov
Date: 01 Sep 00 09:14:56 -0700
Subject: Re: Help with TEM of Mg alloy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

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The Microscopy ListServer -- Sponsor: The Microscopy Society of America


I would like to suggest all those people who are working on the
metallurgy of Mg alloys to refer that original paper published in English. I
apologize that I don't have any reprints of that paper and I don't remember the
formula either, since I've left there for a long time.
Regards,
Zhiping

} From: Milo Kral {m.kral-at-mech.canterbury.ac.nz}
} To: Zhiping Luo {zhiping_luo-at-hotmail.com}
} Subject: Re: Help with TEM of Mg alloy
} Date: Fri, 01 Sep 2000 07:50:04 +1200
}
} Just one more thing - could you send the formula for the electrolyte
} and the polishing conditions to me via e-mail?
}
} Thanks
} Milo
}
} } Dear Milo,
} }
} } To prepare TEM thin foil samples and also the samples for optical
} } metallography, please refer a paper from our group (S.Q. Zhang,
} } Acta
} } Metall. Sinica, 1990, Vol. 3A, p.110), which contains details for
} } the electrolytes. Those electrolytes worked well for all Mg alloys we
} } studied. However, they should NOT be deposited after the experiment
} } since they are explosive after some time especially at higher
} } temperatures.
} }
} } Another way is just to use the conventional ion milling. I didn't
} } detect any clear beam damage by the ion milling even at room
} } temperature.
} }
} } In Mg-based alloys there are many complex intermetallics (Luo et
} } al., Scripta Metall. Mater., 1993, Vol. 28, p. 1513) and I observed
} } a number of new microstructures including the new Mg-Zn-rare earth
} } quasicrystals by TEM.
} }
} } Good luck in your work.
} }
} } Zhiping Luo
} }

} } } From: Milo Kral {m.kral-at-mech.canterbury.ac.nz}
} } } To: Microscopy-at-sparc5.microscopy.com
} } } Subject: Help with TEM of Mg alloy
} } } Date: Thu, 31 Aug 2000 09:36:15 +1200
} } }
} } } -----------------------------------------------------------------------
-
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} } } America



From daemon Fri Sep 1 09:52:57 2000



From: Debby Sherman :      sherman-at-btny.purdue.edu
Date: 01 Sep 2000 09:44:44 -0500
Subject: Quant. Fluorescent microscope

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Hi All,
I have had a request to explore obtaining a fluorescent microscope with appropriate add-ons for doing quantitation of fluorescence. I know nothing about this and would appreciate advise on where to start looking. Would anyone who has such a system please contact me with general information about the system as well as as idea of cost. An investigator is writing a grant and would like to include such a system in the grant.
Thanks,
Debby

Debby Sherman, Manager Phone: 765-494-6666
Microscopy Center in Agriculture FAX: 765-494-5896
Dept. of Botany & Plant Pathology E-mail: sherman-at-btny.purdue.edu
Purdue University
1057 Whistler Building
West Lafayette, IN 47907-1057




From daemon Fri Sep 1 09:55:48 2000



From: emxray-at-uwindsor.ca
Date: Fri, 1 Sep 2000 10:47:30 -0400
Subject: SEMCO Nanolab SEM

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J. W. Robinson
Mechanical and Materials Engineering
University of Windsor
Windsor, Ontario
N9B 3P4

Phone : 519-253-4232, ext 2598

Does someone have an address or phone number for Al Bingham of
Semoptics or someone else who does service on the Nanolab SEM's? Any help
would be appreciated.




From daemon Fri Sep 1 12:38:47 2000



From: Larry D. Ackerman :      mishot-at-itsa.ucsf.edu
Date: Fri, 01 Sep 2000 09:59:35 -0700
Subject: Linux Imaging

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


It is not clear to me what you mean exactly by imaging programs. Do you
mean programs for acquiring images or programs for manipulating existing
images. For the later purpose, the Corel Graphics Suite (includes Draw and
Photo Paint) is very powerful and I use it often rather than the Adobe
Photoshop and Illustrator programs. Caveat: I have not used these programs
on the Linus platfrom myself.


Larry D. Ackerman
Lily & Yuh Nung Jan Laboratories
Howard Hughes Medical Institute
UCSF, Box 0725, Rm U226
533 Parnassus Ave.
San Francisco, CA 94143

(415) 476-8751 FAX (415) 476-5774
mishot-at-itsa.ucsf.edu



From daemon Fri Sep 1 14:39:20 2000



From: Gregory M Fahy :      gregoryfahy-at-juno.com
Date: Fri, 1 Sep 2000 04:27:11 -0700
Subject: FF-FE SEM: Need help with Freeze Etching

Contents Retrieved from Microscopy Listserver Archives
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Is there anyone in Southern California who does
freeze fracture/freeze etch SEM? I would like to
find a collaborator or even better a service lab that
I can send samples to for FF/FE work.

Gregory M. Fahy
21st Century Medicine
________________________________________________________________
YOU'RE PAYING TOO MUCH FOR THE INTERNET!
Juno now offers FREE Internet Access!
Try it today - there's no risk! For your FREE software, visit:
http://dl.www.juno.com/get/tagj.



From daemon Fri Sep 1 15:52:49 2000



From: Gang Ning :      gning-at-mcw.edu
Date: Fri, 01 Sep 2000 15:47:32 -0500
Subject: Testis and kidney ultrastructure

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi -

I have a project which needs to see the preservation of testis and
kidney morphology of rats. I want to set up a couple of ultrastructural
criteria for this. Can any of you provide me some info about good
classical reference books or literature. Any experience or suggestions
are greatly appreciated.

Regards

Gang Ning

EM Facility
Medical College of Wisconsin
Milwaukee, WI 53045
414-456-8141
414-456-6517 (Fax)




From daemon Fri Sep 1 16:27:26 2000



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Fri, 01 Sep 2000 14:17:02 -0700
Subject: Re: SEMCO Nanolab SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Dr. Robinson,
There is a web site for Semoptics at: http://www.magma.ca/~semoptx/ and the
phone and fax number listed there is the same as before: (613) 727-1698.
At 10:47 AM 9/1/00 -0400, you wrote:
} Phone : 519-253-4232, ext 2598
}
} Does someone have an address or phone number for Al Bingham of
} Semoptics or someone else who does service on the Nanolab SEM's? Any help
} would be appreciated.
}
}
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca




From daemon Fri Sep 1 17:58:51 2000



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Fri, 01 Sep 2000 15:49:35 -0700
Subject: Re: SEMCO Nanolab SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Dr. Robinson,
There is a web site for Semoptics at: http://www.magma.ca/~semoptx/ and the
phone and fax number listed there is the same as before: (613) 727-1698.
At 10:47 AM 9/1/00 -0400, you wrote:
} Phone : 519-253-4232, ext 2598
}
} Does someone have an address or phone number for Al Bingham of
} Semoptics or someone else who does service on the Nanolab SEM's? Any } help
would be appreciated.
}

Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca




From daemon Fri Sep 1 17:58:53 2000



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Fri, 01 Sep 2000 15:47:50 -0700
Subject: Re: SEMCO Nanolab SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Dr. Robinson,
There is a web site for Semoptics at: http://www.magma.ca/~semoptx/ and the
phone and fax number listed there is the same as before: (613) 727-1698.
At 10:47 AM 9/1/00 -0400, you wrote:
} Phone : 519-253-4232, ext 2598
}
} Does someone have an address or phone number for Al Bingham of
} Semoptics or someone else who does service on the Nanolab SEM's? Any help
} would be appreciated.
}

Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca




From daemon Fri Sep 1 19:43:22 2000



From: Ken Converse :      qualityimages-at-netrax.net
Date: Fri, 01 Sep 2000 20:21:42 -0700
Subject: Re: SEMCO Nanolab SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


emxray-at-uwindsor.ca-at-sparc5.microscopy.com wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} J. W. Robinson
} Mechanical and Materials Engineering
} University of Windsor
} Windsor, Ontario
} N9B 3P4
}
} Phone : 519-253-4232, ext 2598
}
} Does someone have an address or phone number for Al Bingham of
} Semoptics or someone else who does service on the Nanolab SEM's? Any help
} would be appreciated.


J. W.,
Give Derek Saunders a call. He's in Nepean, Ontario at Electron Optics
Service, Inc. e-mail eos-at-magma.ca. I believe he bought all the
remaining NanoLab stock and is very familiar with their equipment

Ken Converse
owner
Quality Images
Delta, PA 17314

717-456-5491
717-456-7996 fax



From daemon Mon Sep 4 03:10:01 2000



From: Labar Janos :      labar-at-mfa.kfki.hu
Date: Mon, 4 Sep 2000 10:29:00 +0200
Subject: Elliptical distortion in SAED + Help

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Fellow Microscopists,

Elliptical distortion in SAED ring patterns is successfully taken into
account in the newest version of the free ProcessDiffraction program.
Calibration of the eccentricity and direction of axes is performed and
integration goes along ellipses (as contrasted to circles). The program
converts your SAED ring pattern (measured in a TEM) into and XRD-like
distribution of intensity as a function of scattering vector (proportional
to the radius of the ring). d-values and intensities are measured and
listed. Markers show positions of known phases. And more ...

Previous problems with missing Help were also corrected for. (There was a
mismatch between the name of the installed Help-file and the name the
program looked for as Help.) Now the Help works fine and the Help system
explains with illustrations what is the program good for and how to use it.

The program can be downloaded FREE from
http://www.mfa.kfki.hu/~labar/ProcDif.htm .
Steps of installation are also explained there. I suggest to uninstall
previous versions and install the newest (V1.1.0) version.

I hope you will find this program useful and easy to use. Any comments,
suggestions are welcome.

János

Dr. Janos L. Labar
Research Institute for Technical Physics and Materials Science
H-1121 Budapest, Konkoly-Thege u. 29-33
Postal address: H-1525 Budapest-114, Po Box 49
Tel: (36)(1) 392-26-92
Fax: (36)(1) 275-49-96
Fax/phone: (36)(1) 395-92-32
home page: www.mfa.kfki.hu/~labar





From daemon Mon Sep 4 08:07:40 2000



From: Sidorov, Max :      msv-at-nl.feico.com
Date: Mon, 4 Sep 2000 14:52:44 +0200
Subject: this is only a test, sorry

Contents Retrieved from Microscopy Listserver Archives
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Sorry just checking...
Tried to post something the other day but it didn't work.

----------------------------
Dr. Maxim V. Sidorov
TEM Applications Specialist
Philips Electron Optics, Applications Laboratory
Building AAE, Achtseweg Noord 5
5600 MD Eindhoven, the Netherlands

e-mail: msv-at-nl.feico.com
Phone: +31-40/2766101
Fax: +31-40/2766102




From daemon Mon Sep 4 17:01:55 2000



From: Michael Reiner :      Elektronenmikroskopie-at-web.de
Date: Mon, 4 Sep 2000 23:48:31 +0200
Subject: Fwd: EM: Artifacts in myelin sheaths

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} Hello dear microscopists!
}
} I have a question regarding artifacts in the myelin sheath of a peripheral nerve:
} Samples were immersionfixed in Karnovsky´s half-strength fixative(2% Glut, 2% PFA in 0.1M Cacodylate)en bloc stained with osmiumtetroxide and UAc and embedded in Araldite.
} The myelin sheaths show the following appearance:
} 1. The fine lammellar structure is focally disturbed, the layers appear weavy, wobbly ...
} 2. The outer rings are stained more intense than the inner rings (to the axon).
} 3. The myelin sheaths are de-rounded, but this seems to be not so grave and I noted this often in other pictures published. Maybe this is "normal"?
}
} I would appreaciate any suggestions from you all to overcome this problem.
} Thanks in advance!
} Greetings,
} Michael
}
}
} Michael Reiner
} University of Cologne, Germany
} Dept. of Anatomy I

Michael Reiner
University of Cologne, Germany
Dept. of Anatomy I
_______________________________________________________________________
1.000.000 DM gewinnen - kostenlos tippen - http://millionenklick.web.de
IhrName-at-web.de, 8MB Speicher, Verschluesselung - http://freemail.web.de




From daemon Tue Sep 5 05:29:33 2000



From: Sidorov, Max :      msv-at-nl.feico.com
Date: Tue, 5 Sep 2000 12:02:39 +0200
Subject: TEM - Contrast Transfer Function Freeware (ctfExplorer)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear All:
I wrote a piece of software which I believe would be of interest to the
microscopy community.
It's a highly interactive program which calculates/displays the contrast
transfer function of TEMs. I know that there are similar programs floating
around but ctfExplorer does not only 1d but also 2d calculations/display
with 2-fold and 3-fold astigmatism imposed. There are other unique features
to it. All parameters (defocus, voltage, Cs, etc) can be changed
interactively.

DISCLAIMER: I do work for FEI/Philips Electron Optics. However, this
software is not an official product of FEI and FEI is not responsible for
its distribution/support. This software is freeware.

To the best of my knowledge, the software does not have any nasty bugs. It
is tested under Windows 95/98 and Windows NT4.

Please give it a try. Please direct your suggestions and comments to
maxsidorov-at-bigfoot.com
I hope you'll find the software useful.

Here is the link: http://clik.to/ctfexplorer

Enjoy,

Max Sidorov
---
TEM Applications Specialist
FEI/Philips Electron Optics
Eindhoven, The Netherlands
e-mail: maxsidorov-at-bigfoot.com

(Sorry again for my previous "test" post. Apparently my e-mail program was
not set up correctly.)




From daemon Tue Sep 5 10:56:27 2000



From: Peter Sterling :      peter.l.sterling-at-boeing.com
Date: Tue, 05 Sep 2000 08:45:11 -0700
Subject: Linds to Info on Low Vacuum Electron Microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have just recently received and had installed a JEOL 5900LV sem and
although training for this instrument is only a week away, I would like
to do some basic study on the low vacuum aspect of electron microscopy.
I am very familiar with high vac operation of sem's in general but lack
knowledge of the techniques and uses of low vac. Any pointers to web
sites that have a basic to advanced discussion of this subject would be
greatly appreciated.

Peter Sterling
SEM Lab, Structural Materials Labs
Materials and Processes
Rocketdyne Division, The Boeing Company
(818) 586-1434




From daemon Tue Sep 5 11:04:20 2000



From: Weiming Yu :      w-yu-at-northwestern.edu
Date: Tue, 05 Sep 2000 10:52:06 -0500
Subject: Open Microscopist Position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Open Position for an Electron Microscopist
at Northwestern University Medical School

We have an opening for an Electron Microscopy Technologist in the Cell
Imaging Facility of Northwestern University Medical School, hosted
within the Department of Cell and Molecular Biology. This is a
multi-user facility equipped with modern microscopy instruments and
available for researchers of the whole university. The responsibilities
of this position include: EM technical service; Supervising facility's
daily activities; training users (EM, confocal and deconvolution
microscopy, microinjection, and digital image analysis); maintenance of
equipment; helping with developing new microscopy techniques; ordering
supplies, and repairs; instrument scheduling and billing, updating web
page and helping with reports. The preferred qualifications include:
bachelor's degree in life sciences or the equivalent combination of
education, training and experience from which comparable skills can be
acquired; The candidates need to have electron microscopy and
ultramicrotomy experience. Experiences with fluorescence, confocal and
deconvolution microscopy, microinjection, image analysis and computer
skills are very desirable. Salary is commensurate with experience and
education. Please send applications to Dr. Weiming Yu, Department of
Cell and Molecular Biology, Northwestern University Medical School, 303
E. Chicago Ave., Chicago, IL 60611.
Detailed information about the Cell Imaging Facility can be found at
http://www.basic.nwu.edu/corefacilities/cellimaging.html and
http://www.cmb.nwu.edu/cif.html
This is a full time position attached with full benefit package, which
can be found at http://www.northwestern.edu/hr/benefits/


Weiming Yu, PhD
Director, Cell Imaging Facility
Northwestern University
Medical School
303 E. Chicago Ave. W7-159
312/503-2841 fax:312/503-7912



From daemon Tue Sep 5 11:44:41 2000



From: Susan Carbyn :      CarbynS-at-em.agr.ca
Date: Tue, 05 Sep 2000 12:35:15 -0400
Subject: Prices

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello!

Our admin people have decided to bar code all the equipment here, and need to have costs associated with the coding for security reasons. The following is a list of equipment that was either given to us, or we lost the paper trail and I'm having a hard time finding out prices for them. Prices and approximate dates of purchase, not estimated costs of present day value. If anyone has records of the same equipment and could give me a price and approximate purchase date that I could pass onto them, I would greatly appreciate it.

Edwards Coating System E306A Purchased in 1983
Reichert-Jung Ultracut E Microtome
Sonicleaner Dawe Type 6443AE (rough price of a sonicator)
LKB 2178 Knifemaker II
Fisher slide warmer model 77 cat 12-594
Phillips EM-410 TEM

Thanks,
Susan

Susan Carbyn
Electron Microscopy Technician
Atlantic Food and Horticulture Research Centre
Agriculture and Agri-Food Canada
32 Main Street, Kentville, Nova Scotia B4N 1J5
Canada

Phone: (902) 679-5566
Fax: (902) 679-2311

E-mail: carbyns-at-em.agr.ca




From daemon Tue Sep 5 15:34:54 2000



From: MICHAEL DELANNOY :      delannoy-at-mail.jhmi.edu
Date: Tue, 5 Sep 2000 16:21:30 -0400 (EDT)
Subject: equipment

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Does anyone know of a place where one can cell used equipment?

thanks
Mike D




From daemon Tue Sep 5 16:31:46 2000



From: Rosemary Walsh :      rw9-at-psu.edu
Date: Tue, 5 Sep 2000 17:52:18 -0400
Subject: References on bacteria isolated from bog sediments

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers,
I'll be preparing samples of bacteria culture anaerobically
and/or microanaerobically from sediments obtained at different bog sites
in central Pa. I am looking for reference material for use with a class of
graduate students. I expect to use conventional sample prep and an SEM
to image Arthrobacter and Streptomyces. I am also looking for current
literature on Aquaspirillum(magnetotactic bacteria).
Rosemary

Rosemary Walsh, Manager
The Electron Microscope Facility for the Life Sciences,
A Shared Technology Facility, The Life Sciences Consortium
1 South FrearLab
Penn State University
University Park, PA 16802
(814) 865-0212
rw9-at-psu.edu
http://www.lsc.psu.edu/stf/em/home.html





From daemon Wed Sep 6 02:31:50 2000



From: Joergen Bilde-Soerensen 5802 :      j.bilde-at-risoe.dk
Date: Wed, 06 Sep 2000 09:18:19 +0200
Subject: Re: Links to Info on Low Vacuum Electron Microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Peter Sterling wrote:
} I am very familiar with high vac operation of sem's in general but lack
} knowledge of the techniques and uses of low vac. Any pointers to web
} sites that have a basic to advanced discussion of this subject would be
} greatly appreciated.

Hi Peter,

There is a "poor vacuum sem" users site at:

http://www.geocities.com/CapeCanaveral/3429/PVSEM.html

I have a few pages about EDS in LVSEM and ESEM at the
address:

http://www.risoe.dk/afm/news1new.htm

Best regards,
Jørgen

*****************************
J. B. Bilde-Soerensen
Senior Research Scientist, Ph. D.
Materials Research Department
Risoe National Laboratory
DK-4000 Roskilde
Denmark

e-mail: j.bilde-at-risoe.dk
phone: +45 46 77 58 02 (direct)
phone: +45 46 77 46 77 (switchboard)
fax: +45 46 77 57 58
website: http://www.risoe.dk/afm/Personal/jqbi/jqbi.htm



From daemon Wed Sep 6 03:16:43 2000



From: =?iso-8859-1?q?Ian=20MacLaren?= :      maclariz-at-yahoo.co.uk
Date: Wed, 6 Sep 2000 09:04:58 +0100 (BST)
Subject: Modern ion beam thinners

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear all,
Out of interest (i.e. I am not in a position to buy one right now), I am
interested in people's opinion on ion beam thinners on the market today.

I have used a Gatan PIPS in the past and have been very satisfied with this
including many of the features including the low angle milling and the ability
to mount a TV camera to watch the milling in real time (great for hole
detection on transparent ceramics that defeat auto-termination systems).

I know, however, that Gatan are not the only manufacturers of ion-beam thinning
equipment and I am sure that their competitors must also have some decent
machines. So, please tell me what else there is on the market (since I already
know plenty about the Gatan PIPS), what specifications these machines have, and
about your experiences with them. Also, comparisons between different
makes/models of ion beam thinner would be interesting.

Looking forward to hearing from you.

Best wishes

=====
Ian MacLaren
Beijing Laboratory of Electron Microscopy
Chinese Academy of Sciences, P.O. Box 2724
100080 Beijing, China
Email: ian.maclaren-at-physics.org / ianmaclaren-at-hotmail.com
Home page: http://members.tripod.co.uk/IanMacLaren/

____________________________________________________________
Do You Yahoo!?
Get your free -at-yahoo.co.uk address at http://mail.yahoo.co.uk
or your free -at-yahoo.ie address at http://mail.yahoo.ie



From daemon Wed Sep 6 04:46:39 2000



From: Gary Dietrich Chinga :      garyc-at-stud.ntnu.no
Date: Wed, 6 Sep 2000 11:33:28 +0200 (MET DST)
Subject: Osmium and Paper staining

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hi!

I am using Osmium to stain the binders in the coating layer. As you know,
coated paper is composed of fibres (mechanical andchemical) and the
coating
layer (pigment minerals and binders). In order to try to predict how
printing inks will interact with the paper surface during printing it is
very important to characterise the coating layer. The quantification of
the binders (styrene/butadiene latex) is thus important. How to do
that?? I use to embedd paper samples in epoxy resin, take
cross-section images in the SEM,BEI mode and use image analysis
to quantify the binder distribution. However, it is very difficult to see
any
contrast between the binders in the coating layer and the epoxy resin. So,
I stain the paper samples in Osmium before embedding in epoxy. The paper
samples are placed in a staining chamber with a solution of 100 mg OsO4 in
10 ml water. The samples are not in contact with the solution in order to
avoid
structural changes in the paper cross-section. The samples are then vapour
stained for 48 hrs. I get very nice results improving the contrast
between
the latex and the epoxy and also the mechanical (lighter) and chemical
fibres. However, I have experienced a certain roughening of the paper
surface due possibly to the water vapour inside the chamber. Now my
question, how can I avoid this effect, Is it possible to dissolve OsO4 in
another
solvent?. I also tried to stain the samples with crystalline Osmium
(without water), but the results are not so satisfactory.

Any help will be appreciated.

Gary.




From daemon Wed Sep 6 07:09:09 2000



From: didier goux :      didier.goux-at-unicaen.fr
Date: Wed, 6 Sep 2000 13:52:06 -0700
Subject: Help Unicryl

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Microscopist

We persist to use Unicryl for cryo embedding (UV) while many problems.

For in situ hybridization, the section of 3 mycron are put on polylysine
covered glasses

this sections go away (floating) in SDS and SSC bath at 55 degres

how could we "fixe" the sections on glasses?

thank you for reply

Didier


} } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } }
Didier Goux
CENTRE DE MICROSCOPIE ELECTRONIQUE
Université de CAEN Tel : (33) 02.31.56.58.13
Campus I Fax : (33) 02.31.56.56.00
Esplanade de la Paix 14032 CAEN CEDEX
mailto:didier.goux-at-unicaen.fr








From daemon Wed Sep 6 07:09:09 2000



From: donald j marshall :      dmrelion-at-world.std.com
Date: Wed, 6 Sep 2000 07:50:31 -0400 (EDT)
Subject: glass thicknesses

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi, Some time in the middle of the summer I put out a post seeking
information on safe values for vacuum viewing port glass thicknesses, i.e.,
data on thickness of glass versus unsupported diameter for various glasses
with atmospheric pressure on one side and vacuum on the other. Particular
interest is in leaded glass and quartz and in small diameters in the range
of 1 to 3 cm. I didn't get any responses but my guess is that a lot of list
members were not monitoring the microscopy list as frequently as they
normally would. So, with your indulgence, the question is reposted.

Thanks.

Don Marshall

Donald J. Marshall
Relion Industries
P.O. Box 12
Bedford, MA 01730
Ph: 781-275-4695
FAX: 781-271-0252
email dmrelion-at-world.std.com

Cathodoluminescence, mass spectroscopy, electron beam technology


"A weed is a flower out of place."

(Please note: Do not send email with attachments to this address. Instead, send it to donbarlen-at-aol.com. Thank you.)



From daemon Wed Sep 6 08:27:48 2000



From: A.Walker :      Alan.Walker-at-sheffield.ac.uk
Date: Wed, 6 Sep 2000 14:15:25 +0100
Subject: Modern ion beam thinners

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Ian,

I have not used a Gatan PIPS but here at Sheffield University SERC
FEGTEM Facility (http://www.shef.ac.uk/uni/academic/D-H/eee/) we have
both the Leica EMRES 100 and the Technoorg Linda IV3 with an
additional low energy gun.
The Leica is good for general fast milling - it is completely
computer controlled, with video camera viewing and end point
determination, on-board timer and, in our case, Peltier cooling of
the sample holder. You can just set the thing going and leave it for
an hour or two.
The Technoorg Linda, though not computer controlled, is more
versatile, having both the facility to use a low energy ion gun and
liquid nitrogen cooling to the sample holder - topping up is the
problem here.
Neither company has a factory in the UK (Leica's instrument is made
by Balzers in Lichtenstein and Technoorg Linda are Hungarian) but
both are very helpful when it comes to problem solving. Leica have a
UK based rep. - not very useful in China I don't suppose! My point
is that they are willing to give every possible assistance by email or
phone or, if necessary, a site visit.

Hope this helps.

Kind regards,

Alan Walker

*********************************************
Alan Walker
Dept of Electronic and Electrical Engineering
University of Sheffield
Mappin Street
Sheffield S1 3JD
United Kingdom

Tel:+44-(0)114-2225365
Fax:+44-(0)114-2726391
alan.walker-at-shef.ac.uk
http://borg.shef.ac.uk/fegtem/index.htm
*********************************************



From daemon Wed Sep 6 08:38:03 2000



From: A.Walker :      Alan.Walker-at-sheffield.ac.uk
Date: Wed, 6 Sep 2000 14:15:25 +0100
Subject: Modern ion beam thinners

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Gary Dietrich Chinga wrote:

} Hi!
}
} I am using Osmium to stain the binders in the coating layer. As you know,
} coated paper is composed of fibres (mechanical andchemical) and the
} coating
} layer (pigment minerals and binders). In order to try to predict how
} printing inks will interact with the paper surface during printing it is
} very important to characterise the coating layer. The quantification of
} the binders (styrene/butadiene latex) is thus important. How to do
} that?? I use to embedd paper samples in epoxy resin, take
} cross-section images in the SEM,BEI mode and use image analysis
} to quantify the binder distribution. However, it is very difficult to see
} any
} contrast between the binders in the coating layer and the epoxy resin. So,
} I stain the paper samples in Osmium before embedding in epoxy. The paper
} samples are placed in a staining chamber with a solution of 100 mg OsO4 in
} 10 ml water. The samples are not in contact with the solution in order to
} avoid
} structural changes in the paper cross-section. The samples are then vapour
} stained for 48 hrs. I get very nice results improving the contrast
} between
} the latex and the epoxy and also the mechanical (lighter) and chemical
} fibres. However, I have experienced a certain roughening of the paper
} surface due possibly to the water vapour inside the chamber. Now my
} question, how can I avoid this effect, Is it possible to dissolve OsO4 in
} another
} solvent?. I also tried to stain the samples with crystalline Osmium
} (without water), but the results are not so satisfactory.
}
} Any help will be appreciated.
}
} Gary.

Osmium will dissolve in several organic solvents, carbon tetrachloride comes
to mind.

Why dissolve it at all? I suspect that osmium crystals in a sealed container
would produce enough vapor to do the job. Gentle warming would speed things
up.


Geoff
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************




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Hi Ian,

I have not used a Gatan PIPS but here at Sheffield University SERC
FEGTEM Facility (http://www.shef.ac.uk/uni/academic/D-H/eee/) we have
both the Leica EMRES 100 and the Technoorg Linda IV3 with an
additional low energy gun.
The Leica is good for general fast milling - it is completely
computer controlled, with video camera viewing and end point
determination, on-board timer and, in our case, Peltier cooling of
the sample holder. You can just set the thing going and leave it for
an hour or two.
The Technoorg Linda, though not computer controlled, is more
versatile, having both the facility to use a low energy ion gun and
liquid nitrogen cooling to the sample holder - topping up is the
problem here.
Neither company has a factory in the UK (Leica's instrument is made
by Balzers in Lichtenstein and Technoorg Linda are Hungarian) but
both are very helpful when it comes to problem solving. Leica have a
UK based rep. - not very useful in China I don't suppose! My point
is that they are willing to give every possible assistance by email or
phone or, if necessary, a site visit.

Hope this helps.

Kind regards,

Alan Walker

*********************************************
Alan Walker
Dept of Electronic and Electrical Engineering
University of Sheffield
Mappin Street
Sheffield S1 3JD
United Kingdom

Tel:+44-(0)114-2225365
Fax:+44-(0)114-2726391
alan.walker-at-shef.ac.uk
http://borg.shef.ac.uk/fegtem/index.htm
*********************************************



From daemon Wed Sep 6 08:56:02 2000



From: Tamara Howard :      howard-at-cshl.org
Date: Wed, 6 Sep 2000 09:42:37 -0400 (EDT)
Subject: Re: Help Unicryl

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Have you tried using some other coating on your glass slides? Or putting
the section on "Plus" slides (I use Fisher Scientific's, but I'm sure
other companies have similar slides). I'm not sure the PLL is the best
choice for this - I'd go with Plus slides or some "old-fashioned" type of
subbing. I can send protocols if you are interested.

Good Luck!

Tamara Howard
CSHL

On Wed, 6 Sep 2000, didier goux wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear Microscopist
}
} We persist to use Unicryl for cryo embedding (UV) while many problems.
}
} For in situ hybridization, the section of 3 mycron are put on polylysine
} covered glasses
}
} this sections go away (floating) in SDS and SSC bath at 55 degres
}
} how could we "fixe" the sections on glasses?
}
} thank you for reply
}
} Didier
}
}
} } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } }
} Didier Goux
} CENTRE DE MICROSCOPIE ELECTRONIQUE
} Université de CAEN Tel : (33) 02.31.56.58.13
} Campus I Fax : (33) 02.31.56.56.00
} Esplanade de la Paix 14032 CAEN CEDEX
} mailto:didier.goux-at-unicaen.fr
}
}
}
}
}
}
}





From daemon Wed Sep 6 08:56:45 2000



From: Greg Erdos :      gwe-at-biotech.ufl.edu
Date: Wed, 06 Sep 2000 09:47:14 -0400
Subject: M&M Proceedings

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does anyone know if the M&M 2000 proceedings have been mailed yet.??
Greg
Gregory W. Erdos, Ph.D.
Assistant Director, Biotechnology Program
PO Box 110580
University of Florida
Gainesville, FL 32611

Ph. 352-392-1295 Fax: 352-846-0251



From daemon Wed Sep 6 09:01:47 2000



From: anderron-at-us.ibm.com
Date: Wed, 6 Sep 2000 12:17:48 -0400
Subject: Re: M&M Proceedings

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Don,

You can probably go pretty thin with sapphire, given your small diameters
and resulting pressures of less than 50 pounds. Most materials should
work.

Call Melles Griot to ask about burst pressure and strength modulus for
their products. That will help. Their contact information is:

E-mail: optics-at-irvine.mellesgriot.com

Call 1-800-835-2626 or 949-261-5600 and ask for the Optics Specialist

Fax 1-949-261-7790, attention Optics Specialist

Good luck,

Nathan Haese
Lafayette, CA



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Message-ID: {002501c01806$67f42da0$c0a017c3-at-anatomip}


I think that there is old work (From Afzelius?) in which osmium tetroxide
was used in some organic solvent (carbon tetrachloride or chloroform?) to
fix difficult specimens (eggs ?).
You could check in old texts on TEM - like Pearse's "Histological Techniques
for Electron Microscopy"

Dr. A.P. Alves de Matos
Anatomo-Pathology Department
Curry Cabral Hospital
Lisbon

----- Original Message -----
} From: Gary Dietrich Chinga {garyc-at-stud.ntnu.no}
To: ERIC {biology-at-ucla.edu}
Cc: {microscopy-at-sparc5.microscopy.com}
Sent: Wednesday, September 06, 2000 10:33 AM



They were mailed before the mtg. Contact Bill Bailey or the Springer guy:
Herb Neimerow -at- 212-460-1686



Ron Anderson, IBM, Hopewell Jct., New York, USA. anderron-at-us.ibm.com

IBM Analytical Services; http://www.chips.ibm.com/services/asg




From daemon Wed Sep 6 19:08:09 2000



From: Marek Malecki :      mmm-at-ucsd.edu
Date: Wed, 06 Sep 2000 15:31:01 -0700
Subject: Re: Help Unicryl

Contents Retrieved from Microscopy Listserver Archives
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Didier,
You can use treat glass with 1% silane in acetone to make it sticky.
Marek.



At 9/6/00 Wednesday01:52 PM-0700, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Marek Malecki, M.D., Ph.D.
Director and Principal Investigator

Molecular Imaging Laboratories
University of California at San Diego
address: 1500 Suite Bonner Hall, 9500 Gilman Drive, La Jolla, CA 92093-0368
phone: 8588223373
fax: 8588223715
pager: 8586161420
e.mail: mmm-at-ucsd.edu
e.pager: 1620024619-at-alphapage.airtouch.com
www site: http://mil.ucsd.edu/
ftp site: mil1.ucsd.edu








From daemon Wed Sep 6 19:08:10 2000



From: David Henriks :      Henriks-at-CompuServe.COM
Date: Wed, 6 Sep 2000 18:50:00 -0400
Subject: TEM Specimen Preparation Workshop

Contents Retrieved from Microscopy Listserver Archives
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Workshop Objective
This course will cover all aspects of pre-thinning and focus on final
thinning via Tripod Polishing. Due to the limited class size and the
extensive hands-on opportunities, this course is well suited to novices as
well as advanced specimen preparation technicians. This course has been
updated and expanded from past workshops to include pre-FIB preparation
techniques, post-FIB Plasma Trimming as well as the latest techniques
available in low energy ion milling, plasma cleaning and ion beam sputter
deposition and etching for TEM and SEM samples.

The workshop will be held in San Clemente, CA on October 27-28, 2000.
Please contact me directly for registration information.

Best regards-

David
Writing at 3:28:34 PM on 09/06/2000

***************************************************************************
************************

David Henriks
Vice President TEL: 800-728-2233
(toll free in the USA)
South Bay Technology, Inc. +1-949-492-2600
1120 Via Callejon FAX: +1-949-492-1499
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

***************************************************************************
************************

} } } } } Please visit us at http://www.southbaytech.com { { { { {

Manufacturers of precision sample preparation equipment and supplies for
metallography, crystallography and electron microscopy.



From daemon Wed Sep 6 19:08:10 2000



From: Marek Malecki :      mmm-at-ucsd.edu
Date: Wed, 06 Sep 2000 16:00:35 -0700
Subject: lens control system for JEOL's 1200EX

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Would anyone have for sale or trade-in a lens control system for JEOL's 1200EX?


Marek Malecki, M.D., Ph.D.
Director and Principal Investigator

Molecular Imaging Laboratories
University of California at San Diego
address: 1500 Suite Bonner Hall, 9500 Gilman Drive, La Jolla, CA 92093-0368
phone: 8588223373
fax: 8588223715
pager: 8586161420
e.mail: mmm-at-ucsd.edu
e.pager: 1620024619-at-alphapage.airtouch.com
www site: http://mil.ucsd.edu/
ftp site: mil1.ucsd.edu








From daemon Wed Sep 6 19:08:10 2000



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Wed, 06 Sep 2000 14:02:06 -0700
Subject: Re: Modern ion beam thinners

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Ian,
I have had a VCR Group Ion Mill for several years now and have been very
happy with it. It very seldom needs service, is easy for me to service if
needed, does low-angle and atom thinning, has a liquid N2-cooled stage and
an automatic terminator based on an ion gun, which works fine for
transparent samples. My model is old now and the newer ones are computer
controlled. The VCR Group is now part of South Bay Technologies.
(www.southbay.com)
At 09:04 AM 9/6/00 +0100, you wrote:
}
} Dear all,
} Out of interest (i.e. I am not in a position to buy one right now), I am
} interested in people's opinion on ion beam thinners on the market today.
}
} I have used a Gatan PIPS in the past and have been very satisfied with this
} including many of the features including the low angle milling and the ability
} to mount a TV camera to watch the milling in real time (great for hole
} detection on transparent ceramics that defeat auto-termination systems).
}
} I know, however, that Gatan are not the only manufacturers of ion-beam thinning
} equipment and I am sure that their competitors must also have some decent
} machines. So, please tell me what else there is on the market (since I already
} know plenty about the Gatan PIPS), what specifications these machines have, and
} about your experiences with them. Also, comparisons between different
} makes/models of ion beam thinner would be interesting.
}
} Looking forward to hearing from you.
}
} Best wishes
}
} =====
} Ian MacLaren
} Beijing Laboratory of Electron Microscopy
} Chinese Academy of Sciences, P.O. Box 2724
} 100080 Beijing, China
} Email: ian.maclaren-at-physics.org / ianmaclaren-at-hotmail.com
} Home page: http://members.tripod.co.uk/IanMacLaren/
}
} ____________________________________________________________
} Do You Yahoo!?
} Get your free -at-yahoo.co.uk address at http://mail.yahoo.co.uk
} or your free -at-yahoo.ie address at http://mail.yahoo.ie
}
}
Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca




From daemon Wed Sep 6 19:13:43 2000



From: Rick Harris :      raharris-at-ucdavis.edu
Date: Wed, 06 Sep 2000 16:59:09 -0700
Subject: Siemens Elmiskop II

Contents Retrieved from Microscopy Listserver Archives
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There is a Siemens Elmiskop II TEM that has been mothballed and placed on
display in the lobby of a lecture hall on the University of California,
Davis, campus. Several years ago we taught a course and it was used as the
student scope. Once decommissioned, the power supply and ht cable were
given away to an EM service company and the console was placed in the lobby
as an artifact of the early days of EM on the UC campus. The lecture hall
now belongs to a department that has no interest in keeping the old scope
on display and would like to dispose of it. Is there any interest out
there in this device either for parts or for a display item? If not, it
will be buried at the landfill.

Contact me off-list if you have any interest or suggestions for this scope.


Rick A. Harris, Director
Microscopy and Imaging Facility
Section of Molecular and Cellular Biology
1241 Life Sciences Addition
University of California
Davis, CA
530 752 2914
530 754 7536 fax
http://katie.ucdavis.edu
raharris-at-ucdavis.edu




From daemon Wed Sep 6 22:34:18 2000



From: Vitaly Feingold :      vitalylazar-at-worldnet.att.net
Date: Wednesday, September 06, 2000 10:06 AM
Subject: glass thicknesses

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Donald,

Try the following company to get your answers: Larson Electronic Glass
(Redwood City, CA) Tel. (650)369-6734. E-mail gls2mtl-at-juno.com . I am not
aware of their web site.

Vitaly Feingold
Scientific Instruments and Applications
2773 Heath Lane, Duluth GA 30096
(770)232-7785 ph.
(770)232-1791 fax
-----Original Message-----
} From: donald j marshall {dmrelion-at-world.std.com}
To: Microscopy-at-sparc5.microscopy.com {Microscopy-at-sparc5.microscopy.com}




From daemon Thu Sep 7 06:39:06 2000



From: Michelle.Taurino-at-aventis.com
Date: Thu, 7 Sep 2000 06:27:52 -0500
Subject: M&M Proceedings

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I was wondering the same thing. I haven't received my copy as of 6-Sept-00.

Hope they are mailed shortly.
Michelle Taurino
Aventis Pharmaceuticals
Senior Scientist
Bioimaging and Molecular Histology
Tel-908-231-3357
Fax-908-231-3962
e-mail: Michelle.Taurino-at-aventis.com


-----Original Message-----
} From: Greg Erdos [mailto:gwe-at-biotech.ufl.edu]
Sent: Wednesday, September 06, 2000 9:47 AM
To: Microscopy-at-sparc5.microscopy.com


Does anyone know if the M&M 2000 proceedings have been mailed yet.??
Greg
Gregory W. Erdos, Ph.D.
Assistant Director, Biotechnology Program
PO Box 110580
University of Florida
Gainesville, FL 32611

Ph. 352-392-1295 Fax: 352-846-0251



From daemon Thu Sep 7 08:10:45 2000



From: Larry Allard :      l2a-at-ornl.gov
Date: Thu, 07 Sep 2000 08:59:10 -0400
Subject: RE: M&M Proceedings

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


No one in my group has received theirs either...has *anyone* received
their Proceedings yet?

Larry






} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Dr. Lawrence F. Allard
Senior Research Staff Member
High Temperature Materials Laboratory
Oak Ridge National Laboratory
1 Bethel Valley Road
Bldg. 4515, MS 6064
PO Box 2008
Oak Ridge, TN 37831-6064

865-574-4981
865-576-5413 Fax
allardlfjr-at-ornl.gov



From daemon Thu Sep 7 08:41:38 2000



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Thu, 7 Sep 2000 08:32:00 -0500
Subject: RE: M&M Proceedings

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I got mine right after returning from Philadelphia.

Randy

-----Original Message-----
} From: Larry Allard [mailto:l2a-at-ornl.gov]
Sent: Thursday, September 07, 2000 7:59 AM
To: Microscopy-at-sparc5.microscopy.com


No one in my group has received theirs either...has *anyone* received
their Proceedings yet?

Larry






} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Dr. Lawrence F. Allard
Senior Research Staff Member
High Temperature Materials Laboratory
Oak Ridge National Laboratory
1 Bethel Valley Road
Bldg. 4515, MS 6064
PO Box 2008
Oak Ridge, TN 37831-6064

865-574-4981
865-576-5413 Fax
allardlfjr-at-ornl.gov



From daemon Thu Sep 7 08:46:26 2000



From: Stuart McKernan :      mckernan-at-cems.umn.edu
Date: Thu, 7 Sep 2000 08:32:17 -0500 (CDT)
Subject: Re: M&M Proceedings

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Responding to the message of {v04210101b5dd412fa34d-at-[128.219.47.130]}
from Larry Allard {l2a-at-ornl.gov} :
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} No one in my group has received theirs either...has *anyone* received
} their Proceedings yet?
}
} Larry
}

I received mine very shortly after I returned, but it is possible that I was a
special case :)

Thank you all for attending, and I know next year's program commitee will look
forward to seeing you next year in Long Beach CA.



__________________
Stuart McKernan stuartm-at-tc.umn.edu
Office:(612) 626-7594
IT Characterization Facility, University of Minnesota Desk:(612) 624-6009
100 Union Street S. E., Minneapolis, MN 55455 NEW-} Fax:(612) 625-5368




From daemon Thu Sep 7 09:46:11 2000



From: Sally Burns :      burnssal-at-pilot.msu.edu
Date: Thu, 07 Sep 2000 10:39:58 -0400
Subject: turf grass fixation for TEM

Contents Retrieved from Microscopy Listserver Archives
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I am preparing turf grass samples for TEM. We will be examining the
crowns and lead blades for infectious agents.
Any tidbits on fixation and embedding of this tissue would be appreciated!

My starting point is this protocol, which has been used for wood
samples. If it is overkill, please let me know.

Fix: 2% Glutaraldehyde in 50 mM NaCacodylate pH 7.2, with light vacuum
for 24 hours
Wash: 3X for 1 H each RT in buffer with mild agitation
PostFix: 1% OsO4 in water Overnight at 4C
Wash: 3X for 1H each at RT in water with mild agitation
en bloc stain: saturated UA for 30 min with mild agitation
Dehydration series: 25%, 50%, 75%, 100% Acetone, each 30 min with mild agitation
100% acetone + 1% DMP for 30 min with mild agitation
-- 100% acetone + 1% DMP overnight with mild agitation
100% acetone + 1% DMP overnight with mild agitation
Infiltration:
33% Spurr's in Acetone + 1% DMP for 2 H min with mild agitation
66% Spurr's in Acetone + 1% DMP for 2 H min with mild agitation
100 % Spurr's for 2H with mild agitation
100 % Spurr's Overnight with mild agitation
100 % Spurr's for 8-12 H with mild agitation
100 % Spurr's for 8-12 H with mild agitation
Cast in fresh resin


Thanks, Sally

Sally Burns
Center for Advanced Microscopy
B5 Center for Integrated Plant Studies
Michigan State University
East Lansing, MI 48823
(517) 355-5004
burnssal-at-msu.edu



From daemon Thu Sep 7 10:36:57 2000



From: anderron-at-us.ibm.com
Date: Thu, 7 Sep 2000 11:28:14 -0400
Subject: RE: M&M Proceedings

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I checked with Springer on this. They received a list of 227 names to ship
Proceedings to at the end of June. Shipment occurred about August 1. It
is safe to assume that not all of them have reached their destinations yet.
The form you filled out in the registration bulletin said it would take 4-6
weeks

HERE'S THE IMPORTANT PART: If you signed up as a full meeting registrant
AFTER June 15th (or so) and elected to have the Proceedings shipped,
Springer didn't get the labels yet. I called the Meeting Manager's office
and, after a short chat, got a commitment that they would ship the
additional labels overnight to Springer. Herb, at Springer, committed to
getting the additional proceedings shipped early next week.

Ron Anderson
MSA Pres-Elect


Ron Anderson, IBM, Hopewell Jct., New York, USA. anderron-at-us.ibm.com

IBM Analytical Services; http://www.chips.ibm.com/services/asg




From daemon Thu Sep 7 10:38:21 2000



From: John C. Wheatley :      John.Wheatley-at-asu.edu
Date: Thu, 07 Sep 2000 08:36:19 -0700
Subject: Employment

Contents Retrieved from Microscopy Listserver Archives
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Post-Doctoral Associate

Available with the Center for High Resolution Electron Microscopy at
Arizona State University. Position is sponsored by major chemical company
and primary focus of the research will be the development and application
of environmental electron microscopy to industrially relevant catalyst
systems. Areas of particular interest include the study of phase
transformations under reaction environments, in situ polymerization,
mobility and dynamic microstructural changes. Candidate will have a Ph.D.
in material science, material physics, solid state chemistry or chemical
engineering, with extensive experience in catalyst characterization by
transmission electron microscopy. Experience in the areas of catalyst
synthesis, testing and characterization is preferred. Please submit your
resume together and the names of 3 referees to: Dr. Peter A. Crozier,
Industrial Associates Program, Center for Solid State Science, Arizona
State University, Tempe, AZ 85287-1704, Fax (480) 965-9004, email
crozier-at-asu.edu.


John C. Wheatley
Lab Manager
Arizona State University
Center for Solid State Science
PSA-213
BOX 871704
Tempe, AZ 85287-1704


Phone: (480) 965-3831
FAX: (480) 965-9004
John.Wheatley-at-ASU.Edu





From daemon Thu Sep 7 10:44:03 2000



From: Geoff McAuliffe :      mcauliff-at-UMDNJ.EDU
Date: Thu, 07 Sep 2000 11:38:00 -0400
Subject: Re: Testis and kidney ultrastructure

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Gang Ning wrote:

} Hi -
}
} I have a project which needs to see the preservation of testis and
} kidney morphology of rats. I want to set up a couple of ultrastructural
} criteria for this. Can any of you provide me some info about good
} classical reference books or literature. Any experience or suggestions
} are greatly appreciated.
}
} Regards
}
} Gang Ning
}
} EM Facility
} Medical College of Wisconsin
} Milwaukee, WI 53045
} 414-456-8141
} 414-456-6517 (Fax)

See "The ultrastructure of the rat renal medulla as observed after
improved fixation methods" by S-O. Bohman. J. Ultrastruc. Res 47:329-360,
1974. Beautiful EMs. This paper deals with the medulla for the most part,
renal cortex is not too difficult to fix well. Any edition of "The Kidney"
by Brenner and Rector (see vol. 1) should provide good references.
As far as testis goes, Martin Dym was a major researcher in that field
long ago (20 years or so). A lit search for his name might be productive. Or
look at the list of references at the end of the chapter on male
reproductive system in any good histology text (Bloom and Fawcett, Ham [when
Ham was still writing it]).

Geoff
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************





From daemon Thu Sep 7 11:31:02 2000



From: Debby Sherman :      sherman-at-btny.purdue.edu
Date: Thursday, September 7, 2000
Subject: Fwd: M&M Proceedings

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Please excuse me if you've already received this message - I sent it
yesterday to both servers, but received it from neither, so I think our
system ate it on its way out :)

Tamara

---------- Forwarded message ----------

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


I received my proceedings over 1 week ago....They were no doubt sent 3rd class so may take a number of weeks for all to be delivered depending on where you are in the country.
Debby

--------------------------------------


No one in my group has received theirs either...has *anyone* received
their Proceedings yet?

Larry






} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Dr. Lawrence F. Allard
Senior Research Staff Member
High Temperature Materials Laboratory
Oak Ridge National Laboratory
1 Bethel Valley Road
Bldg. 4515, MS 6064
PO Box 2008
Oak Ridge, TN 37831-6064

865-574-4981
865-576-5413 Fax
allardlfjr-at-ornl.gov






From daemon Thu Sep 7 13:15:26 2000



From: Shalvoy, Richard B **CHES :      RBShalvoy-at-archchemicals.com
Date: Thu, 7 Sep 2000 13:08:39 -0500
Subject: RE: M&M Proceedings

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Mine were here when I returned from the Conference.

Richard Shalvoy
Arch Chemicals
Cheshire, CT

-----Original Message-----
} From: Larry Allard [mailto:l2a-at-ornl.gov]
Sent: Thursday, September 07, 2000 8:59 AM
To: Microscopy-at-sparc5.microscopy.com


No one in my group has received theirs either...has *anyone* received
their Proceedings yet?

Larry






} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Dr. Lawrence F. Allard
Senior Research Staff Member
High Temperature Materials Laboratory
Oak Ridge National Laboratory
1 Bethel Valley Road
Bldg. 4515, MS 6064
PO Box 2008
Oak Ridge, TN 37831-6064

865-574-4981
865-576-5413 Fax
allardlfjr-at-ornl.gov




From daemon Thu Sep 7 16:27:06 2000



From: Gib Ahlstrand :      giba-at-puccini.cdl.umn.edu
Date: Thu, 7 Sep 2000 16:21:33 -0500 (CDT)
Subject: Formvar film stability

Contents Retrieved from Microscopy Listserver Archives
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Dear Microlisters,

I'm having a bit of trouble with Formvar film stability under the TEM beam at 60
kV. The films, attached to copper or nickel grids (cleaned with dilute HCl +
acetone in sonicator same day films picked up) develop holes which grow over a
few minutes time until eventually the entire film more or less disintegrates,
collapses. I also noticed that initially, the films are often pulled away from
the rim of the grid by about one square "hole" away.

I'm using new Formvar 15/95 resin powder and new bottle of ethylene dichloride
solvent, mixing to 0.3% weight to volume. I use "Rite-On" glass slides, rinsed
with ethanol to clean of dust and particulates and air dried, to cast the films.
The films float off easily, and look very clean under the TEM. They were dried
overnight prior to inspection. I take pains to use very clean glassware for
mixing the Formvar solution.

Of course, I could caarbon coat them to provide stability, but that's extra work
and I don't seem to have had this kind of problem before.

I barely recall some discussion here some time ago about how Formvar has
"changed" due to different process of manufacturing, or whatever.

Any clues?

Thanks in advance.

Gib

Gib Ahlstrand
Electron Optical Facility, University of Minnesota, Dept. Plant Pathology
495 Borlaug Hall, St. Paul, MN. USA. 55108 (612)625-8249
612-625-9728 FAX, giba-at-puccini.cdl.umn.edu
http://biosci.umn.edu/MIC/consortium.html




From daemon Thu Sep 7 18:00:18 2000



From: David Henriks :      Henriks-at-CompuServe.COM
Date: Thu, 7 Sep 2000 18:45:58 -0400
Subject: Re: Modern ion beam thinners

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear All:

First, a thank you to Mary for the kind words about our ion milling system.
Just a quick correction, our website is actually at www.southbaytech.com.
Hopefully this will slow down the number of calls we have received asking
us why we are now selling DSL service!

Unfortunately, we do not have any information yet on the website about the
XLA2000 ion mill so you will need to contact me directly on that. There is
some information on the Technorg-Linda Low Energy Mill.

Best regards-

David
Writing at 2:58:23 PM on 09/07/2000

***************************************************************************
************************

David Henriks
Vice President TEL: 800-728-2233
(toll free in the USA)
South Bay Technology, Inc. +1-949-492-2600
1120 Via Callejon FAX: +1-949-492-1499
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

***************************************************************************
************************

} } } } } Please visit us at http://www.southbaytech.com { { { { {

Manufacturers of precision sample preparation equipment and supplies for
metallography, crystallography and electron microscopy.

Message text written by Mary Mager
} ------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Dear Ian,
I have had a VCR Group Ion Mill for several years now and have been very
happy with it. It very seldom needs service, is easy for me to service if
needed, does low-angle and atom thinning, has a liquid N2-cooled stage and
an automatic terminator based on an ion gun, which works fine for
transparent samples. My model is old now and the newer ones are computer
controlled. The VCR Group is now part of South Bay Technologies.
(www.southbay.com) {




From daemon Fri Sep 8 03:20:58 2000



From: PETER HEIMANN :      peter.heimann-at-biologie.uni-bielefeld.de
Date: Fri, 08 Sep 2000 10:04:32 +0200
Subject: Re: Formvar film stability

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


we routinely use formvar at a concentration of 0.5 - 0.7 %. however,
you shurely are aware that the thickness of the film critically
depends on the lowering speed of the fluid (slow = thin; fast =
thick).
if you can manage, coat with carbon afterwards, there are no
disadvantages by doing so, only advantages.
good luck
peter

**************************************************
please reply to: peter.heimann-at-biologie.uni-bielefeld.de ("E-MAIL")

Dr. Peter Heimann
Developmental Biology & Molecular Pathology; W7-107
University of Bielefeld
D 33501 Bielefeld / Germany
phone: xx49(0)521-106-5628 / 5627
FAX : xx49(0)521-106-5654
WEB-Site: http://www.uni-bielefeld.de/biologie/Entwicklungsbiologie
WEB-Site: http://www.uni-bielefeld.de/SFB549
******************************************************



From daemon Fri Sep 8 03:25:35 2000



From: PETER HEIMANN :      peter.heimann-at-biologie.uni-bielefeld.de
Date: Fri, 08 Sep 2000 10:16:28 +0200
Subject: Re: Testis and kidney ultrastructure

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


one of the best techniques to perfuse (testis, but brain and other
organs as well) is published by:
Forssmann, WG et al; 1977; Anat. Rec. 188; (3); 307-
314

for excellent testis-EM look for instance after author
Holstein, A.F..
I for myself use the "forssmann"-technique on mouse
testis with excellent results.

good luck

peter heimann
**************************************************
please reply to: peter.heimann-at-biologie.uni-bielefeld.de ("E-MAIL")

Dr. Peter Heimann
Developmental Biology & Molecular Pathology; W7-107
University of Bielefeld
D 33501 Bielefeld / Germany
phone: xx49(0)521-106-5628 / 5627
FAX : xx49(0)521-106-5654
WEB-Site: http://www.uni-bielefeld.de/biologie/Entwicklungsbiologie
WEB-Site: http://www.uni-bielefeld.de/SFB549
******************************************************



From daemon Fri Sep 8 08:16:05 2000



From: Marie E. Cantino :      cantino-at-uconnvm.uconn.edu
Date: Fri, 8 Sep 2000 09:08:33 -0400
Subject: Re: Formvar film stability

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Gil,

Here in Connecticut it is often difficult to make good formvar films in the
summer, due to high humidity. The films are covered with holes of varying
sizes, even when made in a dry bottles and with freshly opened solvent. I
think this problem is caused by condensation of water on the glass surface,
which is cooled significantly during evaporation of the solvent. It
doesn't take much cooling to get condensation when the ambient humidity is
90%! Perhaps you have tiny pinholes in your films that are enlarging to
become visible under the beam.

Marie

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Dr. Marie E. Cantino
Dept. of Physiology and Neurobiology, U-2131
University of Connecticut
Storrs, CT 06269-2131
Phone: 860-486-3588
Fax: 860-486-1936





From daemon Fri Sep 8 08:52:37 2000



From: phil russell :      prussell-at-ncsu.edu
Date: Fri, 08 Sep 2000 09:48:28 -0400
Subject: SEM position at NCSU

Contents Retrieved from Microscopy Listserver Archives
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Position Open -- Immediately
Microscopy and Microanalysis Research Assistant

An immediate position is open for an SEM microscopist at the North
Carolina State University Analytical Instrumentation Facility (AIF).
Duties and responsibilities include: Operation and maintenance of SEM
instrumentation and sample preparation and analysis equipment;
scheduling of access to and oversight of the above instrumentation; user

training and assistance; assistance with the teaching of electron
microscopy laboratory classes, and analysis of a wide variety of
samples. Qualifications must include a BS or MS or equivalent
experience in a materials related discipline along with one or more
years hands on experience with SEM or related techniques. Required
skills include: extensive hands on experience with SEM and related
techniques and accessories (e.g. specimen preparation and associated
analytical tools). Preferred qualifications include: teaching and user
training; familiarity with modern electronics; and experience with
vacuum systems.
Please send resume and three letters of reference to: Phil Russell,
Director; Analytical Instrumentation Facility; North Carolina State
University; Box 7531, Room 318A EGRC; 1010 Main Campus Drive; Raleigh,
NC 27695-7531 or email PRUSSELL-at-NCSU.EDU.

North Carolina State University is an Equal Opportunity and Affirmative
Action Employer. ADA Accommodations: Phil Russell, prussell-at-ncsu.edu,
919-515-7501. In its commitment to diversity and equity, North Carolina

State University seeks applications from women, minorities, and persons
with disabilities

Phillip E. Russell
Professor, Materials Science and Engineering
Director, Analytical Instrumentation Facility
Box 7531, Room 318 EGRC
1010 Main Campus Drive
North Carolina State University
Raleigh, NC 27695-7531

phone 919 515 7501
fax 919 515 6965
prussell-at-ncsu.edu
http://spm.aif.ncsu.edu/aif





From daemon Fri Sep 8 10:30:03 2000



From: jim :      jim-at-proscitech.com.au
Date: Sat, 9 Sep 2000 01:14:06 +1000
Subject: RE: Formvar film stability

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


You can slightly improve stability under the beam by increasing kV, using a
thinner film (and finer mesh grids), using less beam current and better still
irradiating the grid at very low powers (200x) for a couple of minutes. The
brightness appears great at low powers but actual beam intensity is much
greater at higher powers and the lower power irradiation stabilises the film.
Ultimately you can carbon coat.

BUT WHY are you and most people who really want to use plain plastic films use
Formvar???
Butvar is the strongest and most stable of plastic films and is much more
stable under the beam than Formvar without carbon.
EM techniques (for some obvious reasons) die hard. Butvar has been around for
over twenty years but people keep on going back to the old Formvar references.
Disclaimer: ProSciTech does not care if you buy Butvar or Formvar.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Friday, September 08, 2000 7:22 AM, Gib Ahlstrand
[SMTP:giba-at-puccini.cdl.umn.edu] wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear Microlisters,
}
} I'm having a bit of trouble with Formvar film stability under the TEM beam at
} 60
} kV. The films, attached to copper or nickel grids (cleaned with dilute HCl +
} acetone in sonicator same day films picked up) develop holes which grow over
a
}
} few minutes time until eventually the entire film more or less disintegrates,
}
} collapses. I also noticed that initially, the films are often pulled away
from
}
} the rim of the grid by about one square "hole" away.
}
} I'm using new Formvar 15/95 resin powder and new bottle of ethylene
dichloride
}
} solvent, mixing to 0.3% weight to volume. I use "Rite-On" glass slides,
rinsed
}
} with ethanol to clean of dust and particulates and air dried, to cast the
} films.
} The films float off easily, and look very clean under the TEM. They were
dried
}
} overnight prior to inspection. I take pains to use very clean glassware for
} mixing the Formvar solution.
}
} Of course, I could caarbon coat them to provide stability, but that's extra
} work
} and I don't seem to have had this kind of problem before.
}
} I barely recall some discussion here some time ago about how Formvar has
} "changed" due to different process of manufacturing, or whatever.
}
} Any clues?
}
} Thanks in advance.
}
} Gib
}
} Gib Ahlstrand
} Electron Optical Facility, University of Minnesota, Dept. Plant Pathology
} 495 Borlaug Hall, St. Paul, MN. USA. 55108 (612)625-8249
} 612-625-9728 FAX, giba-at-puccini.cdl.umn.edu
} http://biosci.umn.edu/MIC/consortium.html
}




From daemon Fri Sep 8 10:34:40 2000



From: jfb :      jfb-at-uidaho.edu
Date: Fri, 08 Sep 2000 08:26:27 -0700
Subject: Re: Formvar film stability

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Is coating with carbon more work than remounting a specimen after you
have destroyed it with your beam. Carbon coating is part of the
procedure in my lab, and students know not to stick a grid into the
scope without it. We observe many samples weekly at 120kV without
destroying any. Think of all the contamination you are introducing into
the specimen chamber when you "burn" your film and sample.

Franklin Bailey




From daemon Fri Sep 8 12:57:29 2000



From: Walck, Scott D. :      walck-at-ppg.com
Date: Fri, 8 Sep 2000 13:45:21 -0400
Subject: RE: Modern ion beam thinners

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ian,

I have a BalTec RES 100 and I am extremely pleased with it. There are quite
a few options available with it. Not only have I have prepared very good
TEM samples with it, but I have used it for ion polishing and ion etching of
SEM samples for our High Resolution FESEM. BalTec has a number of holders
for SEM applications. In addition, I can use it to sputter deposit films
onto samples. The newest guns that I just had put on ours our very stable
and settle down very quickly. Whatever changes they made in the software
and hardware dramatically improved the operation over the first generation
guns. I really like saving recipes available that I can reuse in the
programming mode. Although I don't have the opportunity to use it much on
glass samples, the image processing for termination is probably the most
sensitive method that I have used. There are a lot of options that the user
can adjust for optimizing the optical termination through image processing.
Alignment of the guns is extremely easy and accurate. Further, they stay
aligned. If you get the ion deflection system, they have an automatic
alignment system. I bought ours because of the Faraday cup termination that
they have available. However, I hardly use it. With the camera system, it
is easy to watch the screen to determine when it is finished. I have the
remote control/monitoring capability but have not put the system on our LAN
yet. I plan to do that very soon.

If you have a FESEM in the same lab as your TEM, you should consider what
this mill could do for you.

PS Let me tell you that I like the PIPS also. It is very simple to use.
It has one mode that is unique to it, the ability to mill on both top and
bottom of the sample from one direction coming in from the perpendicular
direction to the interface of a cross section. Reza Alani of Gatan has
shown many examples of beautiful samples prepared this way. But, if I were
to make the decision again, I would still go with the BalTec system. It is
versatile and can be used in a high tech way as well as a routine way with
technicians pumping through samples. BalTec has responded to my needs and
have considered things that I have said in terms of their software updates.
I have experimented with some sample preparation techniques for SEM that
look very promising using this mill.

Although I do not have direct experience with the other ion mills on the
market, I have talked extensively with their manufacturers and they all have
some nice features. The low energy guns in the Technorg-Linda system
designed by Barna should be carefully considered. My advice to you is to
consider what you want the mill to do in your lab with your samples and go
from there. You need to weigh what features are available on each and how
they would apply to your samples. You also have to weigh experience with
your users to the level of competency required for each of the mills out
there.

I hope this helps.


-Scott


Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)


"The opinions expressed are those of Scott D. Walck and not of PPG
Industries, Inc. nor of any PPG-associated companies."
--




} -----Original Message-----
} From: Ian MacLaren [mailto:maclariz-at-yahoo.co.uk]
} Sent: Wednesday, September 06, 2000 4:05 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Modern ion beam thinners
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListse} rver/FAQ.html
}
}
} --------------------------------------------------------------
} ---------.
}
}
} Dear all,
} Out of interest (i.e. I am not in a position to buy one right
} now), I am
} interested in people's opinion on ion beam thinners on the
} market today.
}
} I have used a Gatan PIPS in the past and have been very
} satisfied with this
} including many of the features including the low angle
} milling and the ability
} to mount a TV camera to watch the milling in real time (great for hole
} detection on transparent ceramics that defeat
} auto-termination systems).
}
} I know, however, that Gatan are not the only manufacturers of
} ion-beam thinning
} equipment and I am sure that their competitors must also have
} some decent
} machines. So, please tell me what else there is on the
} market (since I already
} know plenty about the Gatan PIPS), what specifications these
} machines have, and
} about your experiences with them. Also, comparisons between different
} makes/models of ion beam thinner would be interesting.
}
} Looking forward to hearing from you.
}
} Best wishes
}
} =====
} Ian MacLaren
} Beijing Laboratory of Electron Microscopy
} Chinese Academy of Sciences, P.O. Box 2724
} 100080 Beijing, China
} Email: ian.maclaren-at-physics.org / ianmaclaren-at-hotmail.com
} Home page: http://members.tripod.co.uk/IanMacLaren/
}
} ____________________________________________________________
} Do You Yahoo!?
} Get your free -at-yahoo.co.uk address at http://mail.yahoo.co.uk
} or your free -at-yahoo.ie address at http://mail.yahoo.ie
}



From daemon Fri Sep 8 17:05:20 2000



From: jmkrupp-at-cats.ucsc.edu (Jon Krupp)
Date: Fri, 8 Sep 2000 14:50:42 -0700
Subject: 8" floppy disks

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi:

Anyone need some 8" floppies and/or storage boxes? Yours for free otherwise
- out they go.

Jonathan Krupp
Microscopy & Imaging Lab
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu





From daemon Fri Sep 8 18:04:34 2000



From: Ellen Lee :      lnlee-at-uclink4.berkeley.edu
Date: Fri, 08 Sep 2000 16:00:30 -0700
Subject: subscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html






From daemon Fri Sep 8 18:16:40 2000



From: Robbin Newlin, :      rnewlin-at-burnham-inst.org
Date: Fri, 08 Sep 2000 16:02:13 -0700
Subject: subscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html







From daemon Fri Sep 8 19:24:14 2000



From: Noreen S. Gilman :      n4xiu-at-gate.net
Date: Fri, 8 Sep 2000 20:10:17 -0400
Subject: subscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html







From daemon Sat Sep 9 08:46:14 2000



From: ay813-at-chebucto.ns.ca () (by way of Nestor J. Zaluzec)
Date: Sat, 9 Sep 2000 08:23:04 -0500
Subject: best lens cleaner?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Email: ay813-at-chebucto.ns.ca
Name: Robert Harnum

State: Nova Scotia

Question: 1. What is the best lens cleaner for cleaning oil immersion
lenses on light microscopes?

2. Where can I obtain a lens cleaner which does not contain any alcohol?






From daemon Sat Sep 9 09:06:54 2000



From: Becky Holdford :      r-holdford-at-ti.com
Date: Sat, 09 Sep 2000 10:44:13 -0500
Subject: North Texas carbon coater help

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



----- Original Message -----
} From: {keg-at-alpha3.csd.uwm.edu}
To: {keg-at-csd.uwm.edu}
Sent: Friday, September 08, 2000 9:23 AM


Hello Tamara and All,
An unfortunate, although interesting predicament. There are three variables
that come to mind which may affect the shipping strategy: temperature
change, pressure change and time. I worry that leaving the samples in
methanol/acetic acid and raising the temperature would lead to denaturation
of protein structure due to overfixation. If the samples could be kept
acceptably cool during transport this might help, but I'm not sure of the
technical difficulties (and expense) associated with overseas transport on
dry ice-I believe Fed Ex has pressurized cargo (which would slow the
sublimation of the dry ice). If these samples could be sent via overnight
express this would probably work.
Otherwise, placing the samples in a buffer solution similar to that
proposed for your incubation protocol(s) for shipping could be a viable
approach. Adding some sodium azide to the buffer for transport to prevent
bacterial contamination would be a good idea in my opinion.
I haven't tried any of these approaches but perhaps someone with more
experience than I will agree/disagree and you will be able to find a
quasi-reliable approach to your problem. Good Luck!
Cheers,
Karl Garsha
----- Original Message -----
} From: Tamara Howard {howard-at-cshl.org}
To: Microscopy Listserver {Microscopy-at-sparc5.microscopy.com} ; Histology
listserver {histonet-at-pathology.swmed.edu}
Sent: Thursday, September 07, 2000 11:18 AM


Hello,
Used equipment can be auctioned off/bidded on at www.biobid.com. It seems
like a nifty idea.
Regards,
Karl Garsha
----- Original Message -----
} From: MICHAEL DELANNOY {delannoy-at-mail.jhmi.edu}
To: {microscopy-at-sparc5.microscopy.com}
Sent: Tuesday, September 05, 2000 3:21 PM


All: does anyone in the North Texas area have a carbon coater capable
of handling an 8-inch wafer? We need to get a wafer coated prior to
some FIB edit and ours has not returned from the vendor.
Please reply to me so we don't clutter up the listserver.

--
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Becky Holdford (r-holdford-at-ti.com)
972-598-1291 (pager)
KFAB Physical Analysis Lab--SEM/FIB/FA
Texas Instruments, Inc.
Dallas, TX
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~





From daemon Sat Sep 9 14:25:22 2000



From: Tom Phillips :      PhillipsT-at-missouri.edu
Date: Sat, 9 Sep 2000 14:00:58 -0500
Subject: Re: best lens cleaner?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have used Sparkle Window cleaner (its the purple one!) with great
success but beware that there are big differences in window cleaner
brands. good luck. tom



} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

--
Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)



From daemon Sat Sep 9 15:07:04 2000



From: Earl Weltmer :      earlw-at-pacbell.net
Date: Sat, 09 Sep 2000 12:48:29 -0700
Subject: Service in Scotland

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All,

I have an Amray 2030 SEM that needs packing for shipment to the United
States.
Will any qualified third party maintenance personnel please contact me.

Thank You,

Earl Weltmer
Scanservice Corp.
(714) 573-9158




From daemon Sun Sep 10 09:10:46 2000



From: ldm postdocs :      ldm3-at-risc4.numis.nwu.edu
Date: Sun, 10 Sep 2000 09:03:32 -0500 (CDT)
Subject: Postdoctoral Position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


A postdoctoral position is available immediately in the area of
Environmental Catalysis. The position will be part of the Institute
for Environmental Catalysis (http://www.iec.nwu.edu) and will involve
TEM, both HREM as well as diffraction (Direct Methods) on oxide
surfaces as a function of gas treatment in collaboration with other
faculty at Northwestern as well as Industry, using the unique UHV-HREM
at Northwestern University (see http://www.numis.nwu.edu). A strong
background in TEM is required, and expertise in UHV and associated
techniques (e.g. XPS) is desirable.

Send applications (by email) to:
Professor L. D. Marks
Department of Materials Science and Engineering
mailto:ldm3-at-apollo.numis.nwu.edu

Include a CV and the names of at least two referees.





From daemon Sun Sep 10 11:30:18 2000



From: Robb Mierzwa :      mierzwa-at-jeol.com (by way of Nestor J. Zaluzec)
Date: Sun, 10 Sep 2000 11:15:59 -0500
Subject: Midwest Microscopy & Microanalysis Society Meeting - Sept. 29 a

Contents Retrieved from Microscopy Listserver Archives
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Friday, September 29, 2000
University of Wisconsin
Memorial Union South
227 North Randall Avenue
Madison, WI 53715 (608) 263-2600

Admission is free with MMMS Membership.
MMMS Membership is $10.00 - new members will be accepted at registration

Meeting Schedule

MORNING COMBINED SESSION, Room, Union South
9:45AM - 10:30AM Registration - Coffee, Juice, Pastries will be provided.

10:30 AM Opening remarks

10:35 AM Dr. Sue Welch, UW-Madison Department of Geology and Geophysics
"The Biogeochemical Cycling in the Tennyson Mine"
Co-authors: Matthias Labrenz, Anne Skatvold, Gregory K. Druschel, David A.
Fowle, Tamara Thomsen-Ebert and Jill Banfield

11:35 AM Dr. Will Bigelow, Professor Emeritus of Materials Engineering,
University of Michigan
"The current state of vacuum science in microscopy, or: why it still takes
so long to pump down to an operating vacuum"

AFTERNOON BIOEMPHASIS SESSION: Room , Union South

1:30 PM Hans Ris, Professor Emeritus, UW Madison.
"3D Imaging of cell structures by high resolution , low voltage , field
emission SEM"

2:00 PM Daryl Meyer, UW Madison
"Multiple labeling for EM: Synthesis and use of colloidal nano-particles of
different metal composition and shape."

2:30 PM Judith Croxdale, UW Madison
"Leaf Surfaces, Microscopy and Thievery useful in Analysis".

3:00 PM Jayne Squirrell, UW Madison
"Application of multiphoton microscopy to the study of embryo development".

AFTERNOON PHYSICAL SCIENCE EMPHASIS SESSION: Room , Union South

1:30 PM Carl Loper, UW-Madison Dept of Material Science & Engineering
"Interaction of Pb and Ca and Its Effect on Graphite Morphology in Cast Irons"
co-authors: Junyoung Park and John Fournelle

2:00 PM Reid Cooper, UW-Madison Dept of Material Science & Engineering
"Redox Reactions in Silicate Melts and Glasses"

2:30 PM John H. Perepezko, UW-Madison Dept of Material Science & Engineering
" Synthesis and Initial Crystallization of Amorphous Al Alloys"
coauthors: R.I. Wu, R. Hebert and Z. Dong, University of Wisconsin-Madison

3:00 PM Thomas F. Kelly, UW-Madison Dept of Materials Sceince &
Engineering, and Imago Scientific Instruments Corporation, Madison, WI
"Local Electrode Atom Probes: Background and Application to Microelectronic
Materials"

3:45 PM CHEESE, CHEESE, & ICE CREAM (remember this is Wisconsin) RECEPTION
-- UNION SOUTH

4:45 PM MEETING ADJOURNS Have a SAFE ride home


Support of this meeting has been provided by: University of Wisconsin
Department of Geology & Geophysics, The Biological & Biomaterials
Preparation, Imaging, & Characterization Laboratory, The Advanced
Microscopy Facility, and "Microscopy Today"


Getting to Madison:

Madison is located in south central Wisconsin and is accessible via several
major highways. Madison is:

- 1 1/2 hour drive from Milwaukee (via Interstate 94)
- 2 1/2 hour drive from Chicago (via Interstate 90)
- 4 1/2 hour drive from Minneapolis/St. Paul (via Interstate 94)
- 2 hour drive from Dubuque (via US 151)

Getting to UW-Madison:

- Take I-90 or US 151 to Hwy 12/18
- Follow Hwy 12/18 exit at Park Street (Exit 261 B)
- Proceed north on Park Street about four miles to Regent Street and turn left.
- Proceed on Regent to Randall St. and Turn Right. (See detail map.)







From daemon Sun Sep 10 12:19:06 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Sun, 10 Sep 2000 12:04:45 -0500
Subject: MMMS Fall Meeting Details on the WWW

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Additional Details (Maps, Speaker Info.. etc...) for the MMMS Fall meeting
are available on the MMMS WWW Site

http://www.msa.microscopy.com/MSALAS/MMMS/MMMSSept2000.html


Nestor
Your Friendly Neighborhood SysOp





From daemon Sun Sep 10 12:24:26 2000



From: Robb Mierzwa :      mierzwa-at-jeol.com
Date: Sun, 10 Sep 2000 12:11:44 -0500
Subject: MMMS Sept. 29 Meeting Details on WWW

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Additional Details (Maps, etc...) for the MMMS Fall meeting
are available on the MMMS WWW Site

http://www.msa.microscopy.com/MSALAS/MMMS/MMMSSept2000.html







From daemon Sun Sep 10 12:32:21 2000



From: Paul Webster :      pwebster-at-hei.org
Date: Sun, 10 Sep 2000 12:19:15 -0500
Subject: Hildy Crowley - In memory...

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Nestor,

Below is a message from the family of Hildy Crowley. I thought it would be
of interest to the many people who subscribe to this list why she signed
off recently, and so abruptly. I asked her son if it would be OK to put it
here. He thought it was a good idea and that it would be what she wanted
as she loved EM so much. If you think it is appropriate I will let you
post it. I know that even though I never met her, your listserver brought
us close and that I will miss her.

Paul Webster



HILDY CROWLEY
29 December 1935 - 7 September 2000

After a long and difficult illness Hildegard Heinrich Crowley died quietly
at home on September 7. Earlier in the week she had given her grandsons,
ages 2 and 4, Batman slippers. The boys showed their Oma (German for
Grandma) how much higher the slippers let them jump. A few days before
that she had received the Morton D. Masur Distinguished Service Award from
the Microscopy Society of America because, through the years her advice
also had allowed many electron microscopists to jump a little higher. With
Hildy's help anyone could jump a little higher.

Hildy was born in Stuttgart, Germany. Her mother, also Hildegard, died
that day. She was raised for several years by aunts and a Grandmother in
North German Pomerania, now a part of Poland. Her father then remarried
and she joined him again. Throughout her life she had frightening wartime
dreams from those days – bombings, straffings, government repression,
malnutrition, and Allied tanks arriving in her town of Eslingen.

Hildy's father, a distinguished aeronautical engineer, was one of the
German scientists brought to the US after the war. Only after many months
could the family join him. Years later she spoke with joy of those early
teen years in America, with plenty of food, nice teachers, and wonderful
friends.

A superb student, Hildy also won prizes at the County Fair for cooking and
sewing, and she was an attendant to the college Homecoming Queen; beauty
contests still were "in" then. After college she taught junior high school
science.

In 1962 Hildy married Tom Crowley, a young medical student. In 1963 she
began working as an electron-microscopy technologist at the University of
Minnesota. She was a perfectionist, and EM is a perfectionist's dream and
nightmare. "I love solving problems", she said, and each EM problem solved
exposed ten new ones. The couple's sons, Christopher and Devin, arrived in
1965 and 1967.

After several years of raising youngsters, Hildy returned to EM at the
University of Colorado Health Sciences Center, then at the National Jewish
Hospital, and for the last decade, at Denver University. Armed only with a
Bachelor's degree, superb intellect, and dogged determination, Hildy became
internationally recognized for solving technical problems in EM, focussing
in recent years on immunocytochemistry. She was especially proud of the
lab's display of technical expertise in two Comparative Neurology articles
(July and September 2000), on which she was a prominent co-author.

Hildy and Tom had moved to Denver for its skiing. Again the technical
perfectionist, she became expert in equipment, waxes, and edge care. Her
skiing continued improving well into her 60's, when she still did the
easier expert runs some 35 days each year.

If Hildy was great at EM, she was even greater at teaching it. Brilliant
undergraduate students came to the lab for Honors Theses. Hildy taught
them biology and EM, of course, but she also supported them through
romantic crises, sometimes helped them find physicians or therapists for
their physical or personal problems, and advised them about their parents'
illnesses or malfeasances. She also fed them delicious home-made cakes at
lab meetings. She at first was miffed when someone called her the "Lab
Mom", until a student said plaintively, "I want you to be our Mom. You're
so much better than my own Mom". She gave students level-headed,
practical, grandmotherly, every-day advice; like her ancestors from the
foggy, cold Baltic, she distrusted "that touchy-feely stuff". The students
went off to fine medical or dental or graduate schools, but when they
visited Denver they often took her to lunch. They invited her to attend,
or to be in, their weddings; one came back to ski with her. After knowing
her, they all jumped a little higher.

Hildy's greatest commitment and contribution, of course, was to her family.
Their loss is too large to speak of here.

Hildy learned in June 1999 that her illness would be terminal, but she
wanted that kept secret from most of her friends, who, at the Portland
Microscopy Society meeting, thought her gift of a limo ride to a fancy
restaurant was just a madcap moment. It was a farewell.

Being Hildy, she worked with Tom to design her own simple headstone, which
was erected a couple of months ago. It says, "You shall live in what you
love." She loved so much, and so many, and so well.
Hildy, with love, farewell. Farewell Oma, Mom, Wife, Sister, Lab Mom,
Scientist, Teacher, Mentor, Friend, Colleague. Our Batman slippers fit
just right. You helped us all jump higher.

-------Tom

Hildy's funeral will be at the First Universalist Church, 4101 East Hampden
Avenue, Denver, at 10 AM Tuesday, September 12. A graveside interment
ceremony at Fairmount Cemetery will follow.

Paul Webster, Ph.D.
Associate Scientist & Director
Ahmanson Advanced Electron Microscopy & Imaging Center
House Ear Institute
2100 West Third St.
Los Angeles, CA 90057

Phone: (213) 273-8026
Fax: (213) 413-6739
e-mail: pwebster-at-hei.org
http://www.hei.org/htm/aemi.htm





From daemon Sun Sep 10 19:15:38 2000



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Mon, 11 Sep 2000 12:06:19 GMT+1200
Subject: Re: Hildy Crowley - In memory...

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Thanks for that, Paul

I had no idea who she was, but I always liked reading her postings,
and her last one was just as gracious as ever.

My sympathy to her family.

rtch




Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand



From daemon Mon Sep 11 03:11:38 2000



From: Geoff Carlson :      geof-at-srv0.dns.ed.ac.uk
Date: Mon, 11 Sep 2000 08:57:32 +0000
Subject: subscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html






From daemon Mon Sep 11 08:55:14 2000



From: Tanya Lemire :      Tanya_Lemire-at-sdstate.edu
Date: Mon, 11 Sep 2000 08:37:23 -0500
Subject: subscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html









Tanya D. Lemire, DVM, Diplomate ACVP

Assistant Professor

Animal Disease Research and Diagnostic Laboratory

Veterinary Science Department

South Dakota State University

Box 2175, North Campus Drive

Brookings, South Dakota 57007-1396

(605) 688-5643 (office)

(605) 688-6003 (fax)

Tanya_Lemire-at-sdstate.edu






From daemon Mon Sep 11 12:11:23 2000



From: Dorota Wadowska :      wadowska-at-upei.ca
Date: Mon, 11 Sep 2000 13:49:18 -0300 (ADT)
Subject: TEM safety

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Everybody!
We have two Hitachii TEM's (H600 and H7000). We use liquid nitrogen
to improve the vacuum (back trap) and to decrease contamination of
the column (front trap). Recently, I have been told by the university
safety officer that I can not use LN in the back trap (I and the
users have to climb on a step ladder and lift LN container to fill
the trap) because of the safety reasons. One of our users strained
her back while filling the back trap and she has been on a sick
leave for some time. They suggested to build a platform in the back
of a microscope so it is more sturdy and safer. Have you ever had
problems of that sort? The lab has been opened for 15 years and
we have never had any accident with LN. Is the platform really safer?
Will it interfere with the performance of the microscope, if it is
metal one? Will performance of the scope be affected if I do not use
LN in the back trap?
Thanks for answers
Dorota



From daemon Mon Sep 11 13:03:07 2000



From: Gerald Harrison :      jerry-at-biochem.dental.upenn.edu
Date: Mon, 11 Sep 2000 13:55:21 -0400
Subject: Alternatives for Freon 113?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello form the Univ. of Penn.,

I apologize in advance for what may well be a very sophomoric question.

I haven't paid attention to what may have been discussed regarding
alternatives to Freon 113 as a final drying agent because I have
scrupulously rationed my supply, ml by ml, for several years. Now I'm into
my final 300 ml and must begin to search for a substitute since it has been
my understanding that Freon 113 is no longer available. I only use very
small volumes at a time and 4x500 ml bottles has lasted me nearly 10
years. Most of my specimens are cell layers or other small (less than 1
cubic mm) tissue samples for SEM.

Any suggestions and information about what is currently used as an
alternative to critical point drying out of Freon 113 exchanged with liquid
CO2 would be greatly appreciated.

Thanks,

Gerald Harrison
Electron Microscope Facility
Dental School Research
University of Pennsylvania



From daemon Mon Sep 11 13:25:18 2000



From: Karen Rethoret :      rethoret-at-yorku.ca
Date: Mon, 11 Sep 2000 14:14:15 -0400 (EDT)
Subject: Low Light Camera Required

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hello,

We are looking for camera suggestions that will handle Low Light, Real
time imaging(both for flurorescence and DIC) in the fluorescence
microscope. Something in the $20K range or less for the system.

High resolution is required also. These images can be extremely dark. The
user prefers also to be able to output the realtime imaging of cell growth
to videotape as that has proved to be the most convenient in the past but
is also interested in the "firewire" option and it's capabilities.

We realize that a compromise in some areas (likely the frame rate)will be
required but high resolution during low light imaging is very important.

We would be particularly interested in hearing from others that have used
a camera that would suit our application. We have commercial info for
several models but would really like some user feedback.

Thanks,

Karen Rethoret
York University
Biology Department
Toronto, Ontario
416-736-2100 x33289






From daemon Mon Sep 11 13:36:24 2000



From: Michael Reiner :      Elektronenmikroskopie-at-web.de
Date: Mon, 11 Sep 2000 20:27:28 +0200
Subject: In memoriam Hildy Crowley

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Listservers,

I am very sad about the message that reached me today.

Unfortunatly, I knew Hildy only as a member of the listserver and by private emails. She was a source of wisdom in this community.
I told her about technical problems and she repeated promptly. One day a letter from Denver reached me here in Germany with her protocol and I was so proud to receive it. Someone more or less anynomous cared for my problems, that impressed me much.
I feel indeed that she was a very gentle person and I envy everybody who knew her personally.

My deeply sympathy to her family and her friends.

Michael


Michael Reiner
University of Cologne, Germany
Dept. of Anatomy I
_______________________________________________________________________
1.000.000 DM gewinnen - kostenlos tippen - http://millionenklick.web.de
IhrName-at-web.de, 8MB Speicher, Verschluesselung - http://freemail.web.de





From daemon Mon Sep 11 15:11:00 2000



From: Anthony Garratt-Reed :      tonygr-at-mit.edu
Date: Mon, 11 Sep 2000 16:00:48 -0400
Subject: Re: TEM safety (Liq. N2)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have had concerns about this for some while. The difficulty isn't just
with lifting something heavy (which is, most certainly, a major problem),
but also that the heavy thing is inherently hazardous. If you are pouring
liquid up above you, and you spill it, some will almost certainly splash on
you. This is not good if the liquid is nitrogen (or any other cryogen,
amongst other hazardous materials).

Using a step ladder is also not a very safe prodecure, and if you are
leaning to one side to pour from a heavy dewar, it makes it all the more
unstable.

I have built a wooden platform (well, I had the carpenters build it!) for
the worst of our instruments. I used wood because it was much easier than
designing and ordering a custom metal one, but there is no issue of
interfering with the microscope. The arrangement works well. It is very
simple - 2x4's and plywood, painted with floor paint. The carpenters
designed it as they went along.

Another way of dealing with nitrogen filling in a safe way would be to use
a pressurized tank, and fill the trap, x-ray detector or whatever through a
pipe. The arrangement could be made portable. I have had in mind
researching the cost of a system like this.

I wouldn't recommend an automatic fill system, though. The valves tend to
stick in the open position, draining your liquid nitrogen supply and
freezing (and possibly damaging) the items onto which the liquid spills.
I'm talking from experience here!

Good luck!

Tony.


} Hi Everybody!
} We have two Hitachii TEM's (H600 and H7000). We use liquid nitrogen
} to improve the vacuum (back trap) and to decrease contamination of
} the column (front trap). Recently, I have been told by the university
} safety officer that I can not use LN in the back trap (I and the
} users have to climb on a step ladder and lift LN container to fill
} the trap) because of the safety reasons. One of our users strained
} her back while filling the back trap and she has been on a sick
} leave for some time. They suggested to build a platform in the back
} of a microscope so it is more sturdy and safer. Have you ever had
} problems of that sort? The lab has been opened for 15 years and
} we have never had any accident with LN. Is the platform really safer?
} Will it interfere with the performance of the microscope, if it is
} metal one? Will performance of the scope be affected if I do not use
} LN in the back trap?
} Thanks for answers
} Dorota
}

** Anthony J. Garratt-Reed
** MIT Room # 13-1027
** 77 Massachusetts Avenue
** Cambridge, MA 02139-4307
** USA
**
** Phone: (+) 1-617-253-4622
** Fax: (+) 1-617-258-6479
**





From daemon Mon Sep 11 15:22:58 2000



From: Steve Barlow :      sbarlow-at-sunstroke.sdsu.edu
Date: Mon, 11 Sep 2000 13:20:29 -0700
Subject: Philips 300 TEM for donation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


This is a second posting.

Dr. David Kirk has a Philips 300 TEM available for donation. The
recipient will pay all moving charges. If interested, please contact him
directly

David Kerk, Ph.D.
Professor and Chair
Department of Biology
Point Loma Nazarene University
3900 Lomaland Dr
San Diego, CA 92106
Ph: 619-849-2398
FAX: 619-849-2598
email: dkerk-at-ptloma.edu





From daemon Mon Sep 11 15:50:29 2000



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Tue, 12 Sep 2000 08:48:40 GMT+1200
Subject: PCD for JEOL

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



This is the last posting, I promise, seeking a used (of course no
objection to new) Probe Current Detector (ie Faraday Cup device) for
a JEOL 840 or 6400.

Anyone got one pining away in a cupboard?

cheers

rtch

Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand



From daemon Mon Sep 11 16:59:58 2000



From: Gerald Harrison :      jerry-at-biochem.dental.upenn.edu
Date: Mon, 11 Sep 2000 17:48:10 -0400
Subject: Re: Alternatives for Freon 113?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thanks to all for the very helpful advice,

It seems my fears were unfounded as it relates to small volume laboratory
use. It is still possible to obtain Freon 113, in modest amounts, for
laboratory work.

Gerald Harrison
Electron Microscope Facility
Dental School Research
University of Pennsylvania
=================================





From daemon Mon Sep 11 17:58:54 2000



From: Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 11 Sep 2000 15:47:54 -0700
Subject: Re: Alternatives for Freon 113?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I buy F113 fro Re-Cycle & Re-Use Industries, Mansfield, TX

Try John Metz
817.477.5207

I have about 2 gallons in stock and use maybe a quart or so a year.

gary


At 10:55 AM 9/11/00, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From daemon Mon Sep 11 18:40:12 2000



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Mon, 11 Sep 2000 16:26:48 -0700
Subject: Re: TEM safety

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Dorota,
I sympathize, since I have the H-800, which is the taller, 200 kV version of
the H-600 and I have found that short people do have some problems filling
the back trap. You are lucky you don't have the EDS, which is even higher up.
My solution is to have a self-pressurized dispenser on the 50L liquid
nitrogen dewar attached to a hose that can reach the back trap. Position the
hose, turn on the liquid N2 slowly, close the tap when the trap is full and
wait for the hose to soften before moving it.
There is no reason a platform wouldn't work, so long as it is made of
non-magnetic materials, such as plastic, aluminum or wood.
At 01:49 PM 9/11/00 -0300, you wrote:
}
} Hi Everybody!
} We have two Hitachii TEM's (H600 and H7000). We use liquid nitrogen
} to improve the vacuum (back trap) and to decrease contamination of
} the column (front trap). Recently, I have been told by the university
} safety officer that I can not use LN in the back trap (I and the
} users have to climb on a step ladder and lift LN container to fill
} the trap) because of the safety reasons. One of our users strained
} her back while filling the back trap and she has been on a sick
} leave for some time. They suggested to build a platform in the back
} of a microscope so it is more sturdy and safer. Have you ever had
} problems of that sort? The lab has been opened for 15 years and
} we have never had any accident with LN. Is the platform really safer?
} Will it interfere with the performance of the microscope, if it is
} metal one? Will performance of the scope be affected if I do not use
} LN in the back trap?
} Thanks for answers
} Dorota
}
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca




From daemon Mon Sep 11 22:59:22 2000



From: Gordon Couger :      gcouger-at-couger.com
Date: Mon, 11 Sep 2000 22:44:27 -0500
Subject: Re: TEM safety (Liq. N2)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



----- Original Message ----- }
} I have had concerns about this for some while. The difficulty isn't
just
} with lifting something heavy (which is, most certainly, a major
problem),
} but also that the heavy thing is inherently hazardous. If you are
pouring
} liquid up above you, and you spill it, some will almost certainly splash
on
} you. This is not good if the liquid is nitrogen (or any other cryogen,
} amongst other hazardous materials).
}
} Using a step ladder is also not a very safe prodecure, and if you are
} leaning to one side to pour from a heavy dewar, it makes it all the more
} unstable.
}
} I have built a wooden platform (well, I had the carpenters build it!)
for
} the worst of our instruments. I used wood because it was much easier
than
} designing and ordering a custom metal one, but there is no issue of
} interfering with the microscope. The arrangement works well. It is
very
} simple - 2x4's and plywood, painted with floor paint. The carpenters
} designed it as they went along.
}
} Another way of dealing with nitrogen filling in a safe way would be to
use
} a pressurized tank, and fill the trap, x-ray detector or whatever
through a
} pipe. The arrangement could be made portable. I have had in mind
} researching the cost of a system like this.

Building a portable LN pump would be pretty simple. You need a lid for the
LN container, a wet line to the bottom of the container, a very small
heating element and a vapor vent to stop the transfer.

To use the pump LN fill the tank and put the lid on with the vapor valve
open. The output of the wet line is connected and the vapor valve closed
and the heater turned on. A 500 Ohm resistor and 6 volts should make a
good enough heater. Keep the resistor very small so it will cool rapidly.
To stop the transfer open the vapor valve and turn off the heater. Once
the vapor valve is open the liquid should drain back down the wet line and
everything should be ready to disconnect in a minute or so. The only
problem is if you get into a siphon situation. If you do that you need a
way to relieve the suction.

On vapor releif and suction releif valves I would not totaly rely on
something that could freeze up but I would add a breakable vapor reief
valve that a sharp rap with a stick would beak and shut the system down.

I have rigged a lot of pumps that work this way using about 4 psi. The
original pump I built was for pumping diesel wiht propane and if your
pressure was over 4 PSI you desolved propane in the diesel and vapor
locked your injectors. I have used in in a lot of situations for moving
liquids. I built one that went in bung of a 55 gallon barrel and was
completly sealed except for the pressure releif valve that should be open
at all time when the pump is not in use.

The head on this type pump should not be very great or the transfer rate
is slow, you waste a lot of vapor when you vent and the pressure can get a
higher than is safe. For low head pumping 4 psi will pump 50 gallons of
deisel through a 1.5 inc hose 10 or 15 minutes at a 18 inch head. or 5 or
10 minutes with a -10 inch head.





From daemon Tue Sep 12 00:25:03 2000



From: Gordon Vrololjak :      gvrdolja-at-nature.Berkeley.EDU
Date: Mon, 11 Sep 2000 22:14:57 -0700 (PDT)
Subject: Re: Alternatives for Freon 113?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,
Isn't there a better alternative to Freon 113? We use ethanol for
critical point drying in our lab with fine results. Freon is bad for the
ozone layer. I've copied below, some information from the US
Environmental Protection Agency.

C. Abiotic Effects

Freon 113 moves slowly through the lower atmosphere into the
stratosphere. Photodegradation of freon 113 in the upper atmosphere
releases chlorine atoms which react with ozone. Stratospheric
depletion of ozone increases the amount of ultraviolet-B radiation
that reaches the earth's surface (U.S. EPA 1983). Increased,
surface UV radiation can adversely affect human health and the
environment.

\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
Gordon Ante Vrdoljak Electron Microscope Lab
ICQ 23243541 http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
gvrdolja-at-nature.berkeley.edu UC Berkeley
phone (510) 642-2085 Berkeley CA 94720-3330
fax (510) 643-6207 cell (510) 290-6793

On Mon, 11 Sep 2000, Gary Gaugler wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I buy F113 fro Re-Cycle & Re-Use Industries, Mansfield, TX
}
} Try John Metz
} 817.477.5207
}
} I have about 2 gallons in stock and use maybe a quart or so a year.
}
} gary
}
}
} At 10:55 AM 9/11/00, you wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Hello form the Univ. of Penn.,
} }
} } I apologize in advance for what may well be a very sophomoric
} } question.
} }
} } I haven't paid attention to what may have been discussed
} } regarding alternatives to Freon 113 as a final drying agent because I
} } have scrupulously rationed my supply, ml by ml, for several years. Now
} } I'm into my final 300 ml and must begin to search for a substitute since
} } it has been my understanding that Freon 113 is no longer available. I
} } only use very small volumes at a time and 4x500 ml bottles has lasted me
} } nearly 10 years. Most of my specimens are cell layers or other small
} } (less than 1 cubic mm) tissue samples for SEM.
} }
} } Any suggestions and information about what is currently used as
} } an alternative to critical point drying out of Freon 113 exchanged with
} } liquid CO2 would be greatly appreciated.
} }
} } Thanks,
} }
} } Gerald Harrison
} } Electron Microscope Facility
} } Dental School Research
} } University of Pennsylvania
}
}
}




From daemon Tue Sep 12 02:19:09 2000



From: Cameron Hind :      hindc-at-baxter.com
Date: Tue, 12 Sep 2000 08:53:03 -0500
Subject: Alternatives to Critcal point drying

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hi Listservers,

I have a memory lapse-can you remind me of the reagent used as an
alternative to CPD for SEM;or do you know of/recommend any safe (not too
toxic) alternative method to CPD?-any reply would be appreciated

Cameron

Mr. Cameron Hind
Research Scientist
Advanced Technologies group
Baxter R & D Europe S.C.R.L.
Rue du Progrès 7
1400 Nivelles
Belgium

Tel:+32 67 882 511
Fax:+32 67 217 191
e-mail: hindc-at-baxter.com






















From daemon Tue Sep 12 02:29:30 2000



From: Keith Ryan :      kpr-at-pml.ac.uk
Date: Tue, 12 Sep 2000 08:22:00 +0100
Subject: Re: Hildy Crowley - In memory...

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hildy must indeed have been quite a remarkable person. I have
benefited several times over recent years from her replies and advice.
I note that I currently have fifteen of her messages saved. Three of
those were in connection with my own recent enquiry about folds in
semi-thin resin sections. She replied so quickly to additional
mailings when asked about an additional point. Her knowledge of
electron microscopy and specimen processing was very wide-ranging.
She will be missed by this List.

Keith Ryan
Marine Biological Association
Plymouth UK




From daemon Tue Sep 12 03:50:02 2000



From: John OLoughlin :      John.OLoughlin-at-amnch.ie
Date: Tue, 12 Sep 2000 09:41:40 +0100
Subject: Subscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



--




From daemon Tue Sep 12 04:16:23 2000



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Tue, 12 Sep 2000 10:07:55 +0100 (GMT Daylight Time)
Subject: Re: TEM safety

Contents Retrieved from Microscopy Listserver Archives
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We fill our LN dewar on an SEM from a pressurised dewar
fitted with a long metal hosepipe.

Dave


On Mon, 11 Sep 2000 13:49:18 -0300 (ADT) Dorota Wadowska
{wadowska-at-upei.ca} wrote:

} ------------------------------------------------------------------------
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}
} Hi Everybody!
} We have two Hitachii TEM's (H600 and H7000). We use liquid nitrogen
} to improve the vacuum (back trap) and to decrease contamination of
} the column (front trap). Recently, I have been told by the university
} safety officer that I can not use LN in the back trap (I and the
} users have to climb on a step ladder and lift LN container to fill
} the trap) because of the safety reasons. One of our users strained
} her back while filling the back trap and she has been on a sick
} leave for some time. They suggested to build a platform in the back
} of a microscope so it is more sturdy and safer. Have you ever had
} problems of that sort? The lab has been opened for 15 years and
} we have never had any accident with LN. Is the platform really safer?
} Will it interfere with the performance of the microscope, if it is
} metal one? Will performance of the scope be affected if I do not use
} LN in the back trap?
} Thanks for answers
} Dorota
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"




From daemon Tue Sep 12 07:04:43 2000



From: David_Bell-at-millipore.com
Date: Tue, 12 Sep 2000 07:53:52 -0400
Subject: Re: TEM safety (Liq. N2)

Contents Retrieved from Microscopy Listserver Archives
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I would add to Tony's discussion on a wooden platform, that a metal
platform is not advisable in such proximity to an instrument that has both
high voltage and running water. Should wire insulation crack over time due
to spillage of LN2, and you get a water leak while standing on a metal
platform, you won't be in a very good position! In fact, one lab in which
I worked banned the use of all metal step stools in favor of wooden or
plastic ones for such reasons.

On an aside, how large is the trap? Perhaps a smaller dewar for filling
that particular one is in order. You could easily use a thermos sized
dewar (1L) to fill that trap, especially if you are keeping it full on a
regular basis.

HTH,



David Bell
Scientist
Electron Microscopy Lab
Millipore Corporation
80 Ashby Road
Bedford, MA 01730
(781) 533-2108




From daemon Tue Sep 12 08:08:21 2000



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Tue, 12 Sep 2000 13:57:09 +0100 (GMT Daylight Time)
Subject: Re: Alternatives to Critcal point drying

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We use HMDS (Hexamethyldisilazane) from Sigma.

Dave


On Tue, 12 Sep 2000 08:53:03 -0500 Cameron Hind
{hindc-at-baxter.com} wrote:

} ------------------------------------------------------------------------
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} -----------------------------------------------------------------------.
}
}
}
} Hi Listservers,
}
} I have a memory lapse-can you remind me of the reagent used as an
} alternative to CPD for SEM;or do you know of/recommend any safe (not too
} toxic) alternative method to CPD?-any reply would be appreciated
}
} Cameron
}
} Mr. Cameron Hind
} Research Scientist
} Advanced Technologies group
} Baxter R & D Europe S.C.R.L.
} Rue du Progrès 7
} 1400 Nivelles
} Belgium
}
} Tel:+32 67 882 511
} Fax:+32 67 217 191
} e-mail: hindc-at-baxter.com
}
}
}
}
}
}
}
}
}
}
}
}
}
}
}
}
}
}
}
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"




From daemon Tue Sep 12 08:21:42 2000



From: Michelle Peiffer :      mlk101-at-psu.edu
Date: Tue, 12 Sep 2000 09:10:06 -0400
Subject: microsome labelling

Contents Retrieved from Microscopy Listserver Archives
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Hello.

I gather there are not alot of people out there trying to immunolabel
microsomes because I only received one response last time (Thank you Jan),
but I'll post the latest problem in hopes that someone may have some
insight. I am attempting to immunolabel a protein inside sheep liver
microsomes. Jan Leunissen suggested I combine the microsome suspension
with geletin and coat the inside of an eppendorf with the geletin/microsome
film then preembedd label this film.

When I finished, I had very nice geletin interspersed with large areas of
indistinct matter. I think these areas are where the microsomes were,
before the labelling protocol disrupted them. I processed a small amount of
the geletin/microsome from the same tubes before labelling and it looked
very normal: geletin interspersed with large area of microsomes. Does
anyone have any ideas on how to stabilize the microsomes, or where in the
world they are going?

Thank you,

Michelle Peiffer
*************************************************************
Electron Microscope Facility for the Life Sciences
Penn State University Biotechnology Institute
001 South Frear Lab
University Park PA 16802

phone: 814-865-0212
email: mlk101-at-psu.edu
**************************************************************




From daemon Tue Sep 12 08:21:44 2000



From: jim :      jim-at-proscitech.com.au
Date: Tue, 12 Sep 2000 23:09:38 +1000
Subject: RE: Alternatives to Critical point drying

Contents Retrieved from Microscopy Listserver Archives
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I guess that you are thinking of Ted Pella's Peldri. This has not been
available for some years.
You could try to use a solvent drying method, which for some specimens works
surprisingly well.

Place a double layer of filter paper in a glass Petrie dish.
Add sufficient chloroform to well saturate the filter paper.
Move fixed and dehydrated specimen from ethanol onto a microscope slide placed
onto the filter paper.
Close Petrie dish and store in refrigerator for two days until all solvent has
evaporator.
Don't open Petrie dish until the dish has warmed to at least to room
temperature.
Mount and coat specimen etc.

Disclaimer: we do not sell glass Petrie dishes, filter papers, chloroform or
refrigerators. We do sell CPD and Freeze driers!
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Tuesday, September 12, 2000 11:53 PM, Cameron Hind [SMTP:hindc-at-baxter.com]
wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} Hi Listservers,
}
} I have a memory lapse-can you remind me of the reagent used as an
} alternative to CPD for SEM;or do you know of/recommend any safe (not too
} toxic) alternative method to CPD?-any reply would be appreciated
}
} Cameron
}
} Mr. Cameron Hind
} Research Scientist
} Advanced Technologies group
} Baxter R & D Europe S.C.R.L.
} Rue du Progres 7
} 1400 Nivelles
} Belgium
}
} Tel:+32 67 882 511
} Fax:+32 67 217 191
} e-mail: hindc-at-baxter.com
}
}
}
}
}
}
}
}
}
}
}
}
}
}
}
}
}
}
}




From daemon Tue Sep 12 08:39:47 2000



From: Leona Cohen-Gould :      lcgould-at-mail.med.cornell.edu
Date: Tue, 12 Sep 2000 09:31:09 -0400
Subject: Re: Alternatives to Critcal point drying

Contents Retrieved from Microscopy Listserver Archives
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At 8:53 AM -0500 9/12/00, Cameron Hind wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Lee

Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175





From daemon Tue Sep 12 09:07:50 2000



From: greg :      greg-at-umic.sunysb.edu
Date: Tue, 12 Sep 2000 09:58:47 -0400
Subject: Re: Alternatives to Critcal point drying

Contents Retrieved from Microscopy Listserver Archives
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Hi:
The chemical you are looking for is called
Hexamethyldisilazane (HMDS). You can find it in
mist EM catalogues}

Cameron Hind wrote:

} Hi Listservers,
}
} I have a memory lapse-can you remind me of the reagent used as an
} alternative to CPD for SEM;or do you know of/recommend any safe (not too
} toxic) alternative method to CPD?-any reply would be appreciated
}
} Cameron
}
} Mr. Cameron Hind
} Research Scientist
} Advanced Technologies group
} Baxter R & D Europe S.C.R.L.
} Rue du Progrès 7
} 1400 Nivelles
} Belgium
}
} Tel:+32 67 882 511
} Fax:+32 67 217 191
} e-mail: hindc-at-baxter.com

--
Regards,
Gregory Rudomen
Technical Specialist Electron Microscopy
State University of New York at Stony Brook
University Microscopy Imaging Center
Stony Brook, NY 11794-8088
631-444-7372 Greg-at-umic.sunysb.edu
*************************************************
Standard disclaimer: The opinions expressed
in this communication are my own and do
not necessarily reflect those of the University
Microscopy Imaging Center.
*************************************************



From daemon Tue Sep 12 09:10:11 2000



From: Connolly, Brett :      brett_connolly-at-merck.com
Date: Tue, 12 Sep 2000 09:55:54 -0400
Subject: RE: Low Light Camera Required

Contents Retrieved from Microscopy Listserver Archives
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Karen,
You may want to check out the Magnafire "firewire" system from Optronics.
(www.optronics.com)
Brett


Brett M. Connolly, Ph.D.
Merck Research Laboratories
Department of Pharmacology
WP26A-3000
PO Box 4
West Point, PA 19486
Ph. 215-652-2501
FAX 215-652-2075
e-mail: brett_connolly-at-merck.com


} ----------
} From: Karen Rethoret[SMTP:rethoret-at-yorku.ca]
} Sent: Monday, September 11, 2000 2:14 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Low Light Camera Required
}
} ------------------------------------------------------------------------
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} -----------------------------------------------------------------------.
}
}
}
} Hello,
}
} We are looking for camera suggestions that will handle Low Light, Real
} time imaging(both for flurorescence and DIC) in the fluorescence
} microscope. Something in the $20K range or less for the system.
}
} High resolution is required also. These images can be extremely dark. The
} user prefers also to be able to output the realtime imaging of cell growth
}
} to videotape as that has proved to be the most convenient in the past but
} is also interested in the "firewire" option and it's capabilities.
}
} We realize that a compromise in some areas (likely the frame rate)will be
} required but high resolution during low light imaging is very important.
}
} We would be particularly interested in hearing from others that have used
} a camera that would suit our application. We have commercial info for
} several models but would really like some user feedback.
}
} Thanks,
}
} Karen Rethoret
} York University
} Biology Department
} Toronto, Ontario
} 416-736-2100 x33289
}
}
}
}



From daemon Tue Sep 12 09:28:26 2000



From: Tobias Baskin :      BaskinT-at-missouri.edu
Date: Tue, 12 Sep 2000 09:21:02 -0500
Subject: Re: Alternatives to Critcal point drying

Contents Retrieved from Microscopy Listserver Archives
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Greetings,
Several replies have mentioned this mysterious solvent for
CPD, and that it works on some type of specimens. Could someone
outline how this is used (infiltrate and then evap to air, or what?)
and if there is any rhyme or reason about what kinds of specimens
that work???

THanks,
Tobias

}
}
} We use HMDS (Hexamethyldisilazane) from Sigma.
}
} Dave
}
}
} On Tue, 12 Sep 2000 08:53:03 -0500 Cameron Hind
} {hindc-at-baxter.com} wrote:
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} }
} } Hi Listservers,
} }
} } I have a memory lapse-can you remind me of the reagent used as an
} } alternative to CPD for SEM;or do you know of/recommend any safe (not too
} } toxic) alternative method to CPD?-any reply would be appreciated
} }
} } Cameron
} }
} } Mr. Cameron Hind
} } Research Scientist
} } Advanced Technologies group
} } Baxter R & D Europe S.C.R.L.
} } Rue du ProgrËs 7
} } 1400 Nivelles
} } Belgium
} }
} } Tel:+32 67 882 511
} } Fax:+32 67 217 191
} } e-mail: hindc-at-baxter.com
} }
} }
} }
} }
} }
} }
} }
} }
} }
} }
} }
} }
} }
} }
} }
} }
} }
} }
} }
} }
}
} ----------------------------------------
} Patton, David
} Email: David.Patton-at-uwe.ac.uk
} "University of the West of England"

--
_ ____ __ ____ Tobias I. Baskin
/ \ / / \ / \ \ University of Missouri
/ | / / \ \ \ Biological Sciences
/_ / __ /__ \ \ \__ 109 Tucker Hall
/ / / \ \ \
Columbia, MO 65211-7400 USA
/ / / \ \ \ voice:
573-882-0173
/ /____ / \ \__/ \____ fax: 573-882-0123



From daemon Tue Sep 12 09:29:52 2000



From: Petr Schauer :      Petr-at-isibrno.cz
Date: Tue, 12 Sep 2000 16:22:53 +0200
Subject: EUREM12 Proceedings

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Dear Microscopists,

Let me inform you, that the Proceedings of the "12th EUROPEAN
CONGRESS ON ELECTRON MICROSCOPY" (EUREM12), Brno, Czech Republic,
July 9 – 14, are available.

See http://www.eurem2000.isibrno.cz/frmvol4.html .

The EUREM12 Proceedings consist of three printed volumes, one CD-ROM
containing these three volumes (all available now), and the fourth
volume (the Supplement), which will be distributed in October, 2000.
The complete EUREM12 programme, accessible at the
http://www.eurem2000.isibrno.cz/progra.html, brings full details
about the contents of the Proceedings. The Supplement will contain
the post-deadline posters, lectures of the Ernst Ruska Prize winners
and the list of the participants.

The prices are as follows:
Volume I (Biological Sciences)................50 USD
Volume II (Physical Sciences).................50 USD
Volume III (Instrumentation and Methodology)..50 USD
CD-ROM (Volumes I to III).....................10 USD
Volume IV (Supplement)........................15 USD
Packing and postage,CD-ROM(s)..................5 USD
up to 4 books ................................10 USD
more than 4 books ............................10 USD per 4 books

The payment has to be made in advance to the beneficiary:

Czechoslovak Society for EM, Kralovopolska 147, CZ-61264 Brno, Czech
Republic, either via bank transfer as a net payment to Cs. Obchodni
Banka, a.s., Sumavska 33, CZ-61140 Brno, Czech Republic, SWIFT code
CEKO CZ PP BRN, account no. 08867030 or by means of a personal or
company cheque issued to t

The best way for ordering the Proceedings, is using our Web Site at
the http://www.eurem2000.isibrno.cz/frmvol4.html . The e-mail
(eurem2000-at-isibrno.cz) or the fax (++420 5 41514337) orders are also
acceptable. This offer is valid as long as the stock lasts!

With best regards,

Petr Schauer
+---------------------------------------------------------------------+
| Dr. Petr Schauer, Vicechairman of the Or- | tel.: (+420 5) 41514313 |
| ganization Committee of the 12th EUROPEAN | fax : (+420 5) 41514337 |
| CONGRESS ON ELECTRON MICROSCOPY | (+420 5) 41514404 |
| (Brno, Czech Republic, July 9 - 14, 2000) | e-mail: petr-at-isibrno.cz |
| Kralovopolska 147, CZ-612 64 Brno | eurem2000-at-isibrno.cz |
| Czech Republic |www.eurem2000.isibrno.cz |
+---------------------------------------------------------------------+



From daemon Tue Sep 12 09:54:19 2000



From: Gary Gaugler :      gary-at-gaugler.com
Date: Tue, 12 Sep 2000 07:45:44 -0700
Subject: Re: Alternatives for Freon 113?

Contents Retrieved from Microscopy Listserver Archives
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"Better" is rather tough to define--at least in my application
and use. First off, I use about F-113 perhaps six times a
year and use about 20cc of it initially. I dehydrate in
a sequential immersion in methanol, acetone, F-113.
Each immersion is accomplished in a sealed vessel. When
the F-113 has too much gunk on the surface, I skim it off,
put it in a salvage container and continue using the F-113 until
it is too low in volume to be useful. Eventually, I have a pretty
full salvage container. This is sent back to the recycle place
where they clean it and re-sell the F-113. Very little is lost.

An alternative is CPD. However, considering that F-113 costs
about $100 per pound and a CPD setup is perhaps $15K plus
a lot of hassle, CPD is not cost effective or time effective. If I
did dehydration on a regular basis, I would certainly do CPD.

Recycling and containment makes F-113 a low cost and
effective approach to dehydration. And I think the method I
use is environmentally friendly. There are of course ways
to abuse this material. I don't think that I am doing that.

gary g.


At 10:14 PM 9/11/00, you wrote:

} Hello,
} Isn't there a better alternative to Freon 113? We use ethanol for
} critical point drying in our lab with fine results. Freon is bad for the
} ozone layer. I've copied below, some information from the US
} Environmental Protection Agency.
}
} C. Abiotic Effects
}
} Freon 113 moves slowly through the lower atmosphere into the
} stratosphere. Photodegradation of freon 113 in the upper atmosphere
} releases chlorine atoms which react with ozone. Stratospheric
} depletion of ozone increases the amount of ultraviolet-B radiation
} that reaches the earth's surface (U.S. EPA 1983). Increased,
} surface UV radiation can adversely affect human health and the
} environment.
}
} \/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
} Gordon Ante Vrdoljak Electron Microscope Lab
} ICQ 23243541 http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
} gvrdolja-at-nature.berkeley.edu UC Berkeley
} phone (510) 642-2085 Berkeley CA 94720-3330
} fax (510) 643-6207 cell (510) 290-6793
}
} On Mon, 11 Sep 2000, Gary Gaugler wrote:
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } I buy F113 fro Re-Cycle & Re-Use Industries, Mansfield, TX
} }
} } Try John Metz
} } 817.477.5207
} }
} } I have about 2 gallons in stock and use maybe a quart or so a year.
} }
} } gary
} }
} }
} } At 10:55 AM 9/11/00, you wrote:
} } } ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } -----------------------------------------------------------------------.
} } }
} } }
} } } Hello form the Univ. of Penn.,
} } }
} } } I apologize in advance for what may well be a very sophomoric
} } } question.
} } }
} } } I haven't paid attention to what may have been discussed
} } } regarding alternatives to Freon 113 as a final drying agent because I
} } } have scrupulously rationed my supply, ml by ml, for several years. Now
} } } I'm into my final 300 ml and must begin to search for a substitute since
} } } it has been my understanding that Freon 113 is no longer available. I
} } } only use very small volumes at a time and 4x500 ml bottles has lasted me
} } } nearly 10 years. Most of my specimens are cell layers or other small
} } } (less than 1 cubic mm) tissue samples for SEM.
} } }
} } } Any suggestions and information about what is currently used as
} } } an alternative to critical point drying out of Freon 113 exchanged with
} } } liquid CO2 would be greatly appreciated.
} } }
} } } Thanks,
} } }
} } } Gerald Harrison
} } } Electron Microscope Facility
} } } Dental School Research
} } } University of Pennsylvania
} }
} }
} }




From daemon Tue Sep 12 09:57:35 2000



From: Timothy Schneider :      Timothy.Schneider-at-Mail.TJU.EDU
Date: Tue, 12 Sep 2000 10:46:36 -0400
Subject: LIQUID NITROGEN

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Hello:

I also have a Hitachi TEM (7000) and put liquid nitrogen in both the turbo
molecular pump and the anticontamination trap. What I know for sure is that
my Varian 880 vacuum ionization gauge usually reads about one torr better
with the liquid nitrogen then with out it. As a generalization, I have very
little astigmatism and would like to think that the anticontamination trap
is working, but I really don't know that.

Tim

Timothy G. Schneider
Director of Electron Microscopy
Department of Pathology
Room 229 Jefferson Hall
Thomas Jefferson University
1020 Locust St.
Philadelphia Pa. 19107
215-503-4798 work
610-613-8170 cellular
timothy.schneider-at-mail.tju.edu




From daemon Tue Sep 12 10:07:45 2000



From: Robin Cross :      r.cross-at-ru.ac.za
Date: Tue, 12 Sep 2000 16:59:40 +0200
Subject: Re: Alternatives to Critcal point drying

Contents Retrieved from Microscopy Listserver Archives
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Cameron

} I have a memory lapse-can you remind me of the reagent used as an
} alternative to CPD for SEM

It is hexamethyldisilazane (HMDS).

Rob



Robin H Cross
Director : EM Unit, Rhodes University, Grahamstown, South Africa
R.Cross-at-ru.ac.za
tel: +27 46 603 8168 - fax: +27 46 622 4377
http://www.ru.ac.za/emu/index.htm

** remember that ICEM-15 in 2002 is in Durban, South Africa **



From daemon Tue Sep 12 10:45:34 2000



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Tue, 12 Sep 2000 11:38:19 -0500
Subject: Reagents for CPD

Contents Retrieved from Microscopy Listserver Archives
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-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Mr. Cameron Hind wrote:
=============================================================
} I have a memory lapse-can you remind me of the reagent used as an
} alternative to CPD for SEM;or do you know of/recommend any safe (not too
} toxic) alternative method to CPD?-any reply would be appreciated
=============================================================
The current material that is presently available, to my knowledge the only
such material, is HMDS, or Hexamethyldisilazane, otherwise known as 1,1,1,3,
3,3 hexamethyldisilazane
[CAS # 999-97-3].

More information about this material, which is offered by SPI Supplies and
some of the other leading suppliers of chemicals and consumables to the EM
world, can be found on URL
http://www.2spi.com/catalog/chem/chem2a2.html

An opinion: Nothing has ever been developed in the way of a technique of
sample preparation for SEM that is as good as critical point drying. Some
years ago, Ted Pella offered a product called Pel-Dry, it was good, but not
quite as good as actually doing it via CPD. HMDS, again, in my opinion,
falls a bit short of what Pel-Dry used to do. But for someone without a CPD
unit, wanting to prepare wet samples, the HMDS technique is lightyears
better than air drying. I should also say that there are many people who
use HMDS regularly, saying that for their samples, it is "good enough"
either because of the lack of fine structure, or the very low magnifications
they are using.

Disclaimer: SPI has offered for many years our own SPI-Chem HMDS and also a
CPD unit.

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
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Cust.Service: spi2spi-at-2spi.com

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WWW: http://www.spi.cc
########################
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From daemon Tue Sep 12 12:51:12 2000



From: Huggins, Bradley J :      HUGGINBJ-at-bp.com
Date: Tue, 12 Sep 2000 12:34:21 -0500
Subject: RE: turf grass fixation for TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I've been out of touch with EM of membranes and tissues for many many years,
but as I recall acetone as a dehydration agent is rather harsh. After your
fixation and cross-linking steps it may be that a more gently dehydration
would help further preserve your plant tissue. I am definitely not up to
speed on these preps and I am sure some others will respond, but I think I
would try ethanol or another slightly less polar solvent for the early
dehydration steps. I only say this from my (ancient) experience with
acetone and its aggressive extraction behavior to plant tissue and
photosynthetic membranes. Otherwise, I'd stick to your recipe.

Good Luck
Brad Huggins
BP Amoco

} ----------
} From: Sally Burns[SMTP:burnssal-at-pilot.msu.edu]
} Sent: Thursday, September 07, 2000 9:39 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: turf grass fixation for TEM
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I am preparing turf grass samples for TEM. We will be examining the
} crowns and lead blades for infectious agents.
} Any tidbits on fixation and embedding of this tissue would be
} appreciated!
}
} My starting point is this protocol, which has been used for wood
} samples. If it is overkill, please let me know.
}
} Fix: 2% Glutaraldehyde in 50 mM NaCacodylate pH 7.2, with light vacuum
} for 24 hours
} Wash: 3X for 1 H each RT in buffer with mild agitation
} PostFix: 1% OsO4 in water Overnight at 4C
} Wash: 3X for 1H each at RT in water with mild agitation
} en bloc stain: saturated UA for 30 min with mild agitation
} Dehydration series: 25%, 50%, 75%, 100% Acetone, each 30 min with mild
} agitation
} 100% acetone + 1% DMP for 30 min with mild agitation
} -- 100% acetone + 1% DMP overnight with mild agitation
} 100% acetone + 1% DMP overnight with mild agitation
} Infiltration:
} 33% Spurr's in Acetone + 1% DMP for 2 H min with mild agitation
} 66% Spurr's in Acetone + 1% DMP for 2 H min with mild agitation
} 100 % Spurr's for 2H with mild agitation
} 100 % Spurr's Overnight with mild agitation
} 100 % Spurr's for 8-12 H with mild agitation
} 100 % Spurr's for 8-12 H with mild agitation
} Cast in fresh resin
}
}
} Thanks, Sally
}
} Sally Burns
} Center for Advanced Microscopy
} B5 Center for Integrated Plant Studies
} Michigan State University
} East Lansing, MI 48823
} (517) 355-5004
} burnssal-at-msu.edu
}



From daemon Tue Sep 12 14:34:49 2000



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Wed, 13 Sep 2000 07:31:32 GMT+1200
Subject: Anybody else want a PCD?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Further to my posting yesterday seeking a PCD for a JEOL 840, which,
I am informed, is the same as that for a 6400, I am currently
negotiating with a company to make me one.

Does anyone else out there want one?

It would probably ease my negotiations if there were to be more
orders than just mine.

Please contact me direct.

rtch


Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand



From daemon Tue Sep 12 14:37:52 2000



From: Bachman, Georgia (NIDDK) :      gbachman-at-phx.niddk.nih.gov
Date: Tue, 12 Sep 2000 15:30:32 -0400
Subject: help on staining w/ORO

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello, Maybe you can help? I culture human preadipocytes in 35mm dishes
} and then differentiate them to form lipid droplets. I have been staining
} them with ORO for the past year, but have recently not been able to get
the
} ORO to penetrate the cells. I had been fixing the cells with formaldehyde
} and calcium chloride (4g calcium chloride + 10ml of 37%formaldehyde into
} ~90ml water to fix the cells for 1 hour. Then rinse with Hanks buffered
} saline 2x and then stain with ORO for 15min. This has been working for 1
} year, then one day the ORO stain would not penetrate the cells any longer
-
} the lipid is clear, not red. What could be the problem? The formaldehyde
} solution? Have you ever experienced something like this? Thank you for
any
} suggestions.
Also, I have ordered all new fixative, CaCl2, ORO stain powder and
isopropanol. The
} lipid in my cells are not picking up the stain. Oh why, oh why???? I even
} tried the stain on live cells without fixing and they won't stain either.
Thanks for any word.
}
} Just for your information: I use .6g ORO in 100ml 100% isopropanol.

Georgia

Georgia Bachman
NIH - Technical IRTA
Gene Expression Unit
4212 N. 16th St.
Phoenix, AZ 85016
Phone: 602-200-5310 or 5303.




From daemon Tue Sep 12 18:24:47 2000



From: Mark West :      mwest-at-ifcsun1.ifisiol.unam.mx
Date: Tue, 12 Sep 2000 18:14:08 -0600 (=?ISO-8859-1?Q?Hora_est=E1ndar_de_M=E9xico?=)
Subject: serial sections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

I'm having (or trying to have) a go at doing serial ultrathin sections of
Epon-embedded material. I'd like to see a long unbroken ribbon coming off
the diamond knife, but after battling for a couple of hours nothing seemed
to work - the sections floated off happily on their own little journeys
and I wasn't going to chase them around. I tried cutting the pyramid sides
with a new Weckprep blade to get them as smooth as possible, making them
as parallel as railway tracks, lowering and raising the water level in the
trough, and turning off vibrating things in the lab (unfortunately we're
on the third floor), but nothing seemed to make any difference. I'm going
home now (smiling) but would like to hear of any tips and experiences
you've had that got you beautiful ribbons of sections (and any other tips
for serial sectioning).

Looking forward to reading my mail tomorrow.

Mark






********************************************
Mark West,
Unidad de Microscopia Electronica,
(Electron Microscopy Unit)
Instituto de Fisiologia Celular,
Universidad Nacional Autonoma de Mexico,
04510 Mexico D.F.

tel (unidad/lab) *(525) 622 5610*
(casa/home) (525) 619 3020
Fax (525) 616 2282
********************************************




From daemon Tue Sep 12 18:24:47 2000



From: Steve Barlow :      sbarlow-at-sunstroke.sdsu.edu
Date: Tue, 12 Sep 2000 16:16:58 -0700
Subject: need part for Reichert OMU2

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Listers

I am looking for a knifeblock for a Reichert OMU2 to be able to bring one
of these vintage machines back on-line. Should you have this part
currently gathering dust or holding open a door in your lab, please contact
me.

thanks in advance

Steve

Dr. Steven Barlow
EM Facility/Biology Dept.
San Diego State University
5500 Campanile Drive
San Diego CA 92182-4614
phone: (619) 594-4523
fax: (619) 594-5676
email: sbarlow-at-sunstroke.sdsu.edu
http://www.sci.sdsu.edu/emfacility





From daemon Tue Sep 12 21:12:23 2000



From: Sara Miller :      saram-at-duke.edu
Date: Tue, 12 Sep 2000 21:55:07 -0400 (EDT)
Subject: Re: serial sections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On Tue, 12 Sep 2000, Mark West wrote:

} Date: Tue, 12 Sep 2000 18:14:08 -0600 (=?ISO-8859-1?Q?Hora_est=E1ndar_de_M=E9xico?=)
} From: Mark West {mwest-at-ifcsun1.ifisiol.unam.mx}
} To: Microscopy-at-sparc5.microscopy.com
} Subject: serial sections
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi,
}
} I'm having (or trying to have) a go at doing serial ultrathin sections of
} Epon-embedded material. I'd like to see a long unbroken ribbon coming off
} the diamond knife, but after battling for a couple of hours nothing seemed
} to work - the sections floated off happily on their own little journeys
} and I wasn't going to chase them around. I tried cutting the pyramid sides
} with a new Weckprep blade to get them as smooth as possible, making them
} as parallel as railway tracks, lowering and raising the water level in the
} trough, and turning off vibrating things in the lab (unfortunately we're
} on the third floor), but nothing seemed to make any difference. I'm going
} home now (smiling) but would like to hear of any tips and experiences
} you've had that got you beautiful ribbons of sections (and any other tips
} for serial sectioning).
}
} Looking forward to reading my mail tomorrow.
}
} Mark
}
You should trim the top and bottom of your trapezoid **on the microtome
with a glass knife.**

This means that the minute scores caused on the side facet by rough trimming
run parallel to the top and bottom of your block face, not perpendicular
as they do when you trim by hand pushing the razor blade down from the face.

Also, as you said, the top edge and bottom edge should be parallel. But
the biggest thing is not to have any scores caused by a razor blade.

If you don't know how to rough-trim with the microtome, contact me, and
I'll be happy to describe it.


} } } } }
} ********************************************
} Mark West,
} Unidad de Microscopia Electronica,
} (Electron Microscopy Unit)
} Instituto de Fisiologia Celular,
} Universidad Nacional Autonoma de Mexico,
} 04510 Mexico D.F.
}
} tel (unidad/lab) *(525) 622 5610*
} (casa/home) (525) 619 3020
} Fax (525) 616 2282
} ********************************************
}
}
}

Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265




From daemon Wed Sep 13 00:14:38 2000



From: jim :      jim-at-proscitech.com.au
Date: Wed, 13 Sep 2000 13:40:15 +1000
Subject: RE: Alternatives to Critical point drying

Contents Retrieved from Microscopy Listserver Archives
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In my previous response I gave the method, though I've used Chloroform. HMDS
may have a theoretical advantage, but I expect that the results would be
identical.
HMDS was introduced for rapid processing, particularly fast and complete
dehydration. It chemically interacts with water and forms acetone. When at
least the last dehydration is in HMDS, the user can be sure that dehydration is
excellent even with doubtful ethanol or acetone.

During the air drying process residual water and solvent molecules exerts huge
pressures onto membranes and these cause shrinkage and shriveling of any
specimen.
The drying mechanism:
Using Peldri, drying only occurred at the Peldri/ air interface, but the
specimen is held rigid in the solid Peldri material. As the Peldri slowly
sublimes, more of the specimen is dried.
During solvent drying the specimen is in a solvent saturated atmosphere and
drying is very slow - over 48 hours. In this method there is little pressure
exerted by molecules trying to pass through membranes and consequently
shrinkage is minimized.
Its a good alternative method and sometimes works very well. Given a choice
I'll try CPD first.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Wednesday, September 13, 2000 12:21 AM, Tobias Baskin
[SMTP:BaskinT-at-missouri.edu] wrote:
}
}
} Greetings,
} Several replies have mentioned this mysterious solvent for
} CPD, and that it works on some type of specimens. Could someone
} outline how this is used (infiltrate and then evap to air, or what?)
} and if there is any rhyme or reason about what kinds of specimens
} that work???
}
} THanks,
} Tobias
}
} }
} }
} } We use HMDS (Hexamethyldisilazane) from Sigma.
} }
} } Dave
} }
} }
} } On Tue, 12 Sep 2000 08:53:03 -0500 Cameron Hind
} } {hindc-at-baxter.com} wrote:
} }
} } } ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } -----------------------------------------------------------------------.
} } }
} } }
} } }
} } } Hi Listservers,
} } }
} } } I have a memory lapse-can you remind me of the reagent used as an
} } } alternative to CPD for SEM;or do you know of/recommend any safe (not too
} } } toxic) alternative method to CPD?-any reply would be appreciated
} } }
} } } Cameron
} } }
} } } Mr. Cameron Hind
} } } Research Scientist
} } } Advanced Technologies group
} } } Baxter R & D Europe S.C.R.L.
} } } Rue du ProgrEs 7
} } } 1400 Nivelles
} } } Belgium
} } }
} } } Tel:+32 67 882 511
} } } Fax:+32 67 217 191
} } } e-mail: hindc-at-baxter.com
} } }
} } }
} } }
} } }
} } }
} } }
} } }
} } }
} } }
} } }
} } }
} } }
} } }
} } }
} } }
} } }
} } }
} } }
} } }
} } }
} }
} } ----------------------------------------
} } Patton, David
} } Email: David.Patton-at-uwe.ac.uk
} } "University of the West of England"
}
} --
} _ ____ __ ____ Tobias I. Baskin
} / \ / / \ / \ \ University of Missouri
} / | / / \ \ \ Biological
} Sciences
} /_ / __ /__ \ \ \__ 109 Tucker Hall
} / / / \ \ \
} Columbia, MO 65211-7400 USA
} / / / \ \ \ voice:
} 573-882-0173
} / /____ / \ \__/ \____ fax: 573-882-0123
}
}




From daemon Wed Sep 13 01:01:44 2000



From: Sorin Lazar :      sorin-at-ibd.dbio.ro
Date: Wed, 13 Sep 2000 08:51:02 +0300
Subject: Looking for Hitachi S 500 Operating Manual

Contents Retrieved from Microscopy Listserver Archives
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Dear Microscopists,

My name is Sorin Lazar and I am scientist in Bucharest, Romania.
I have received like donation a Hitachi S 500 #01-03 scanning electron
microscope. Unfortunately I don't have the operating manual.
I should be grateful if someone can help me to find an Operating Manual or
a copy.

Thanks in advance,
Sorin





From daemon Wed Sep 13 02:51:00 2000



From: Alexander Mironov :      amironov-at-nki.nl
Date: Wed, 13 Sep 2000 10:39:26 +0200
Subject: Re: serial sections

Contents Retrieved from Microscopy Listserver Archives
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Hi,
Try to trim the upper and the lower edges with a glass knife. Trimming with
razor blade often results in non-perfectly parallel edges.
Turn the block 90 degree (right or left) trim left and right sides with
perfect glass knife. Use both sides of the glass knife. I used the angle of
the knife towards the block around 60 degree. When you trim your block from
both sides just turn it back 90 degree - you will get perfectly parallel
edges.

Your problem could be caused also by irregular air flow around microtome or
some grease on the blade you have used.

Hope it will help.

Sincerely,
--
Alexander Mironov Jr.
Division of Tumor Biology, H4
The Netherlands Cancer Institute
Plesmanlaan 121
1066 CX Amsterdam

Tel. +31-(0)20-512-2017
Fax +31-(0)20-512-2029
E-mail: amironov-at-nki.nl





From daemon Wed Sep 13 03:48:11 2000



From: Steve Chapman :      PROTRAIN-at-CompuServe.COM
Date: Wed, 13 Sep 2000 04:34:23 -0400
Subject: LN2 and Contamination

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi

I have read the offerings with interest and would like to make a few
comments.

Firstly - when a manufacturer designs an instrument they do not fit cold
traps for fun, they believe that to obtain the best performance from their
instrument you need the facilities that they fit. I have worked with
design teams at two manufacturers and this is the attitude that they both
took!

Secondly - I have in the past, in countries where LN2 was not available,
used dry ice (Solid CO2) and acetone in LN2 traps. I believe this brings
the temperature down to -80deg C and it works quite well much better than
no cooling at all.

Thirdly there are imaging techniques that allow us to measure the
contamination rate. Place a holey film in the TEM and without LN2 in use
and take a high resolution picture of a hole. Leave the beam on the
specimen and repeat the picture on the same hole 20 minutes later (that is
if you still have a hole). Measure the two hole widths, take the smaller
from the larger and divide by 2 (2 contaminated edges) divide by the time
between the two pictures and divide by the magnification. You now have
your contamination rate in nm/minute. Then try this with the LN2 systems
in action and you will see just how well the LN2 systems work.

The same system can be used with a SEM but use a well coated latex sphere
in place of the hole. In this case the sphere will grow with time as the
contamination builds round the sphere.

Many years ago (1968) Hitachi produced a paper on LN2 systems. They gist
was that if you used LN2 systems for a period of time and then stopped, it
took an equal period of time before the vacuum level and contamination rate
fell back to the original level. Of course you could not count the time if
you let the column to air.

A few more things to think about and a way of proving just how clean your
microscope is.

Steve Chapman
Senior Consultant Protrain
For consultancy and training by professional World Wide
Tel +44 1280 814774 Fax +44 1280 814007
www.emcourses.com



From daemon Wed Sep 13 07:50:13 2000



From: Geoff Williams :      willi1gl-at-cmich.edu
Date: Wed, 13 Sep 2000 08:38:34 -0400
Subject: Re: turf grass fixation for TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


"Huggins, Bradley J" wrote:

} I've been out of touch with EM of membranes and tissues for many many years,
} but as I recall acetone as a dehydration agent is rather harsh. After your
} fixation and cross-linking steps it may be that a more gently dehydration
} would help further preserve your plant tissue. I am definitely not up to
} speed on these preps and I am sure some others will respond, but I think I
} would try ethanol or another slightly less polar solvent for the early
} dehydration steps. I only say this from my (ancient) experience with
} acetone and its aggressive extraction behavior to plant tissue and
} photosynthetic membranes. Otherwise, I'd stick to your recipe.
}
} Good Luck
} Brad Huggins
} BP Amoco

Brad, (and all)

My experience with Ethanol is that it makes the cell walls extremely brittle and
hard to section, esp in concetrations above 70% ETOH.

Time is always the problem with Plant tissue. The cell walls really like to
hold on to what ever it was in last. So dehydration must proceed at a snails
pace. Grass leaf blades by being very thin make the process easier but I woud
imagine that the crowns would be problematic if carried too fast through
dehydration.

Sincerely,
Geoff Williams

Electron Microscope Facility Supervisor
Biology Department
Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859

Geoffrey.Lloyd.Williams-at-cmich.edu
517 774-3576
517 774-3462 (fax)





From daemon Wed Sep 13 08:01:10 2000



From: Dorothy Roak Sorenson :      dsoren-at-umich.edu
Date: Wed, 13 Sep 2000 08:51:41 -0400 (EDT)
Subject: Re: serial sections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Mark,

To glue serial sections together, make a gentle adhesive by dissolving the
adhesive off of a piece of Scotch tape in chloroform. Paint the upper and
lower block faces with your new adhesive, and, as you cut, the sections
should stick together tenaciously.

Good luck!

Dotty Sorenson
Microscopy and Image Analysis Laboratory
Department of Cell and Developmental Biology
University if Michigan Medical School
Ann Arbor, Michigan
dsoren-at-umich.edu

On Tue, 12 Sep 2000, Mark West wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi,
}
} I'm having (or trying to have) a go at doing serial ultrathin sections of
} Epon-embedded material. I'd like to see a long unbroken ribbon coming off
} the diamond knife, but after battling for a couple of hours nothing seemed
} to work - the sections floated off happily on their own little journeys
} and I wasn't going to chase them around. I tried cutting the pyramid sides
} with a new Weckprep blade to get them as smooth as possible, making them
} as parallel as railway tracks, lowering and raising the water level in the
} trough, and turning off vibrating things in the lab (unfortunately we're
} on the third floor), but nothing seemed to make any difference. I'm going
} home now (smiling) but would like to hear of any tips and experiences
} you've had that got you beautiful ribbons of sections (and any other tips
} for serial sectioning).
}
} Looking forward to reading my mail tomorrow.
}
} Mark
}
}
}
}
}
}
} ********************************************
} Mark West,
} Unidad de Microscopia Electronica,
} (Electron Microscopy Unit)
} Instituto de Fisiologia Celular,
} Universidad Nacional Autonoma de Mexico,
} 04510 Mexico D.F.
}
} tel (unidad/lab) *(525) 622 5610*
} (casa/home) (525) 619 3020
} Fax (525) 616 2282
} ********************************************
}
}




From daemon Wed Sep 13 09:20:14 2000



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Wed, 13 Sep 2000 15:08:06 +0100 (GMT Daylight Time)
Subject: HMDS Shedule

Contents Retrieved from Microscopy Listserver Archives
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For animal tissue:
10min. in 20%,30%,50%,70%,90% and 100% ethanol (or whatever
times etc you use for CPD).
2x10min. in 100% ethanl
10min. in 1:1 ethanol HMDS (or 1:2, 1:1, and 2:1)
3x10min in HMDS
I put tissue on filter paper in a petri dish with lid ajar
and let it evaporate in fume cupboard.

Most papers say CPD is better eg for fine hairs on leaves.
I believe the first report was on very satifactory use on
insects. We use it mostly on bacteria which are pretty
tough anyway. I have found it disappointing on cultured
cells and am planning to dust down the CPD (cryoSEM and ESEM
having relegated it to an historical artifact (sic!) for
most purposes in this lab).

Dave



On Tue, 12 Sep 2000 09:21:02 -0500 Tobias Baskin
{BaskinT-at-missouri.edu} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Greetings,
} Several replies have mentioned this mysterious solvent for
} CPD, and that it works on some type of specimens. Could someone
} outline how this is used (infiltrate and then evap to air, or what?)
} and if there is any rhyme or reason about what kinds of specimens
} that work???
}
} THanks,
} Tobias
}
} }
} }
} } We use HMDS (Hexamethyldisilazane) from Sigma.
} }
} } Dave
} }
} }
} } On Tue, 12 Sep 2000 08:53:03 -0500 Cameron Hind
} } {hindc-at-baxter.com} wrote:
} }
} } } ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } -----------------------------------------------------------------------.
} } }
} } }
} } }
} } } Hi Listservers,
} } }
} } } I have a memory lapse-can you remind me of the reagent used as an
} } } alternative to CPD for SEM;or do you know of/recommend any safe (not too
} } } toxic) alternative method to CPD?-any reply would be appreciated
} } }
} } } Cameron
} } }
} } } Mr. Cameron Hind
} } } Research Scientist
} } } Advanced Technologies group
} } } Baxter R & D Europe S.C.R.L.
} } } Rue du ProgrËs 7
} } } 1400 Nivelles
} } } Belgium
} } }
} } } Tel:+32 67 882 511
} } } Fax:+32 67 217 191
} } } e-mail: hindc-at-baxter.com
} } }
} } }
} } }
} } }
} } }
} } }
} } }
} } }
} } }
} } }
} } }
} } }
} } }
} } }
} } }
} } }
} } }
} } }
} } }
} } }
} }
} } ----------------------------------------
} } Patton, David
} } Email: David.Patton-at-uwe.ac.uk
} } "University of the West of England"
}
} --
} _ ____ __ ____ Tobias I. Baskin
} / \ / / \ / \ \ University of Missouri
} / | / / \ \ \ Biological Sciences
} /_ / __ /__ \ \ \__ 109 Tucker Hall
} / / / \ \ \
} Columbia, MO 65211-7400 USA
} / / / \ \ \ voice:
} 573-882-0173
} / /____ / \ \__/ \____ fax: 573-882-0123
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"




From daemon Wed Sep 13 09:28:18 2000



From: MICHAEL DELANNOY :      delannoy-at-mail.jhmi.edu
Date: Wed, 13 Sep 2000 10:08:02 -0400 (EDT)
Subject: Re: serial sections

Contents Retrieved from Microscopy Listserver Archives
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On Tue, 12 Sep 2000, Mark West wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
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Hello,
You should trim the top and bottom sides of you face with
a glass knife and try coating those sides with grid glue (soak double
stick tape in chloroform, then remove the tape, coat with a filter paper
wedge careful not to wet your face) Good Luck

Mike D.

}
}
} Hi,
}
} I'm having (or trying to have) a go at doing serial ultrathin sections of
} Epon-embedded material. I'd like to see a long unbroken ribbon coming off
} the diamond knife, but after battling for a couple of hours nothing seemed
} to work - the sections floated off happily on their own little journeys
} and I wasn't going to chase them around. I tried cutting the pyramid sides
} with a new Weckprep blade to get them as smooth as possible, making them
} as parallel as railway tracks, lowering and raising the water level in the
} trough, and turning off vibrating things in the lab (unfortunately we're
} on the third floor), but nothing seemed to make any difference. I'm going
} home now (smiling) but would like to hear of any tips and experiences
} you've had that got you beautiful ribbons of sections (and any other tips
} for serial sectioning).
}
} Looking forward to reading my mail tomorrow.
}
} Mark
}
}
}
}
}
}
} ********************************************
} Mark West,
} Unidad de Microscopia Electronica,
} (Electron Microscopy Unit)
} Instituto de Fisiologia Celular,
} Universidad Nacional Autonoma de Mexico,
} 04510 Mexico D.F.
}
} tel (unidad/lab) *(525) 622 5610*
} (casa/home) (525) 619 3020
} Fax (525) 616 2282
} ********************************************
}
}




From daemon Wed Sep 13 10:17:02 2000



From: MICHAEL DELANNOY :      delannoy-at-mail.jhmi.edu
Date: Wed, 13 Sep 2000 10:40:35 -0400 (EDT)
Subject: Re: Formvar film stability

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On Thu, 7 Sep 2000, Gib Ahlstrand wrote:

} ------------------------------------------------------------------------
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} -----------------------------------------------------------------------.
Hello,
Fomvar thickness will effect stability, if you could make them
slightly thicker than gray you should have no problem. If you need them
really thin other than carbom coating you could try minimizing illumination/
intensity on the EM side, smaller spot size, smaller condenser aperture
or lower beam current. good luck.

Mike D
}
}
} Dear Microlisters,
}
} I'm having a bit of trouble with Formvar film stability under the TEM beam at 60
} kV. The films, attached to copper or nickel grids (cleaned with dilute HCl +
} acetone in sonicator same day films picked up) develop holes which grow over a
} few minutes time until eventually the entire film more or less disintegrates,
} collapses. I also noticed that initially, the films are often pulled away from
} the rim of the grid by about one square "hole" away.
}
} I'm using new Formvar 15/95 resin powder and new bottle of ethylene dichloride
} solvent, mixing to 0.3% weight to volume. I use "Rite-On" glass slides, rinsed
} with ethanol to clean of dust and particulates and air dried, to cast the films.
} The films float off easily, and look very clean under the TEM. They were dried
} overnight prior to inspection. I take pains to use very clean glassware for
} mixing the Formvar solution.
}
} Of course, I could caarbon coat them to provide stability, but that's extra work
} and I don't seem to have had this kind of problem before.
}
} I barely recall some discussion here some time ago about how Formvar has
} "changed" due to different process of manufacturing, or whatever.
}
} Any clues?
}
} Thanks in advance.
}
} Gib
}
} Gib Ahlstrand
} Electron Optical Facility, University of Minnesota, Dept. Plant Pathology
} 495 Borlaug Hall, St. Paul, MN. USA. 55108 (612)625-8249
} 612-625-9728 FAX, giba-at-puccini.cdl.umn.edu
} http://biosci.umn.edu/MIC/consortium.html
}
}




From daemon Wed Sep 13 11:11:10 2000



From: John Minter :      john.minter-at-kodak.com
Date: Wed, 13 Sep 2000 12:19:07 -0400
Subject: IMAGE PROCESSING: Warping images to correct barrel distortion

Contents Retrieved from Microscopy Listserver Archives
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Rough trim the excess plastic from the specimen face with a razor blade,
keeping all the tissue edges. Put the specimen chuck into the microtome
arm, and put in a dry glass knife. Shave off enough plastic from the
front (face) in very small increments (start with 1 um slices, decrease
to 0.5 um, then 0.1 um until you get a mirror smooth face). If you must
keep all the sample, then start with a very thin shaving just to get the
face smooth. You should be able to tell when you are into the tissue by
looking at the thin shavings. Mark the top edgeQthe one that will become
the top of your trapezoid--with a magic marker on the chuck. Be sure to
keep track of the plane of your sample because the clearance angle of the
knife will necessitate that you return the specimen to the original
position in which you trimmed the face. Rotate the chuck/specimen 90
degrees (e.g., clockwise). The edges that will become the bottom and top
of your trapezoid are now vertical instead of parallel to the earth.
Turn the knife holder about 30 degrees away from center (e.g., right) and
approach the block carefully. Trim the left side of the block which will
become the bottom of your trapezoid; this will make a smooth facet that
will start with the face and go toward the microtome slanting outward at
about 30 degrees. Leave the specimen in this position and turn the knife
to the other side (e.g., left). Trim this side like the first; it will
become the facet over the top of your trapezoid. The top and bottom
should now be parallel. If they are not, you can make minute adjustments
by slightly rotating the block. The purpose of this exercise it to make
the scratches caused by rough trimming be parallel, not perpendicular as
happens when trimming with a razor blade, to the top and bottom edges of
the face.

If the face is too wide, you can trim it, either on the microtome or with
a razor blade. I usually just chop the sides with a razor blade to save
time; I make a trapezoid with the top edge slightly shorter than the
bottom edge. However, folks with unsteady hands can make the same
trapezoid by turning the block back to its original position with the
magic marker at the top, then slightly rotating it right or left about 2
or 3 degrees. Trim this side with the knife still at 30 degrees away
from center. Then rotate the block 2 or 3 degrees in the other direction
and turn the knife 30 degrees to the opposite side. Trim the other side.

Before sectioning, turn the block back 2-3 degrees to be in the original
position it was when you faced it with the mark at the top. Use a fresh
knife, or even better if you have one, use a diamond. When aligning, be
sure to have both the bottom edge of your block parallel to the edge of
your knife as well as the plane of your block face parallel to the plane
of the knife edge. To do the former, you may have to rotate your
specimen very slightly. To do the latter, you may have to change the
angle of your specimen in the microtome arm. These tiny adjustments may
be necessary because the diamond may not be mounted in its holder exactly
like the glass one you used for trimming.

Pick up the sections on slotted grids with a support film.

Good luck. Let me know if this doesn't make sense.


Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265

---------- Forwarded message ----------


Summary:

I am looking for a test image with control points
with known positions in the warped and unwarped state
and the known coefficients for a polynomial warp
function to test a program I am writing. Can someone on
the list help?

Details:

I am trying to write a program to correct center of
gravity data for barrel distortion in the image. I am trying
to implement the approach described by W. K. Pratt, Digital
Image Processing, 2nd edition, pp.430-433 (1991). I imaged
a rectangular lattice of squares [50 micron squares in a
100 micron square lattice] with a 5X objective. I fit the
measured center of gravity coordinates to a square lattice
using the points nearest the center. The difference between
observed and computed values for each point shows the
barrel distortion in the lens. I used the calculated
value for each point as coordinate for the (unwarped) desired
control point (x,y) and the observed coordinates as the coordinate
for the warped control points (u,v). I'm trying to fit to the
following orthogonal polynomials:

u = a0 + a1x + a2y + a3x**2 + a4xy + a5y**2
v = b0 + b1x + b2y + b3x**2 + b4xy + b5y**2

To do so, I generated the matrix
A,

1 u1 v1 u1**2 u1v1 v1**2
1 u2 v2 u2**2 u2v2 v2**2
A = 1 u3 v3 u3**2 u3v2 v3**2
.....
1 um vm um**2 umv2 vm**2

and the vectors u and v

u(transpose) = [u1 u2 u3 ... um]
v(transpose) = [v1 u2 v3 ... vm]

I used Mathematica to compute the generalized inverse
(Pseudoinverse[]) and generate the vector of coefficients,
a and b by

a = PseudoInverse[A] u
b = PseudoInverse[A] v

The non-unitary coefficients of a and b were much smaller than
I expected and when I tried to use them to correct the center of gravity
data, they did not remove the barrel distortion. I'd like a test
image with known control points that produce known polynomial
coefficients to test the code. Hoping that someone on the list
can help....

Best Regards,

John Minter
Eastman Kodak Company
Analytical Technology Division
Rochester, NY 14650-2152
Phone: (716) 722-3407
FAX: (716) 477-7781
email: john.minter-at-kodak.com



From daemon Wed Sep 13 12:16:37 2000



From: Buckingham, Steve :      sbuckingham-at-excellatron.com
Date: Wed, 13 Sep 2000 13:10:29 -0400
Subject: Microscopy Position

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Hi all,
Thank you all for your input regarding the typical salaries for
Microscopists, it was very useful.
I would now like to request that anyone who might be interested in
an SEM operators position with a start-up company in the Atlanta, GA area of
the USA please send me a copy of your resume and previous salary history.
Although the type of position has not yet been finalized it will probably be
at the entry level. Thank you to those of you who have already sent me their
information, I have it on file for consideration.

Many Thanks,

Steve Buckingham




From daemon Wed Sep 13 12:40:01 2000



From: George McMurtry :      qsales-at-quesant.com
Date: Wed, 13 Sep 2000 11:40:34 -0500
Subject: Customer support position in fastest growing SPM company

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Quesant Instrument Corp is looking for a customer support person whose
primary duties will be:
1. Utilizing Quesant's QScopes (scanning probe microscopes) to scan samples
and report back to sales prospects and customers.
2. Installation and taining of new customers.
3. Attending trade shows and demonstrating QScopes in the booth.
4. Work with marketing and R&D in testing and implementing new features.

Requirements: Advanced degree in Chemistry, Physics or Materials Science
a plus but not mandatory. Must have good interpersonal skills and a profession
al appearance. Must be willing to travel.

Contact Quesant for more information at 29397 Agoura Road, Suite 104
Agoura Hills, CA 91301
tel: 818-597-0311
fax: 818-991-5490
e-mail: qsales-at-quesant.com





From daemon Wed Sep 13 12:40:01 2000



From: David Kerk :      dkerk-at-ptloma.edu
Date: Wed, 13 Sep 2000 11:42:40 -0500
Subject: Reichert Ultracut ultramicrotome available

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We are closing down our EM facility. We have a Reichert Ultracut
ultramicrotome available. Please make an offer, and be prepared to assume
the cost of shipping to your facility.

Thanks,
David Kerk

-------------
David Kerk, Ph.D.
Professor and Chair
Department of Biology
Point Loma Nazarene University
3900 Lomaland Dr
San Diego, CA 92106
Ph: 619-849-2398
FAX: 619-849-2598
email: dkerk-at-ptloma.edu





From daemon Wed Sep 13 13:14:06 2000



From: Debby Sherman :      sherman-at-btny.purdue.edu
Date: 13 Sep 2000 13:00:11 -0500
Subject: Re: LN2 and Contamination

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Steve,
Thanks for this post. I try to get this through to our users and we faithfully use and require use of N2 on all scopes. However, there are other facilities on campus who do not use N2 or use it intermittantly. When this topic comes up again, I intend to hand them your post and ask them to determine contamination rates on their instruments to convince them of the necessity of using N2.
One question that I have is that our main nitrogen trap on the SEM is above the diffusion pump to minimize backstreaming from the pump into the specimen chamber. (We also have a decontaminator close to the specimen that is cooled when we do cryo-SEM.) Now this is no doubt more of a problem with SEM than TEM due to the location of the diffusion pump. However, I wonder why most TEM manufacturers do not also use a nitrogen trap in this location, at least in the higher end scopes, in addition to the decontaminator in the specimen area. It would seem that, since contamination rate in the TEM is likely to be more critical to high resolution, it would also be used to help maintain the best possible vacuum in the column.
I am aware that the majority of the contamination is produced in the TEM by interaction of the beam with the specimen thus the need for decontaminator close to the specimen. However, my understanding is that the better the vacuum, the fewer the interactions of the beam electrons with molecules in the column and the more coherent the beam....i.e. better potential resolution.
Debby

Debby Sherman Phone: 765-494-6666
Microscopy Center in Agriculture FAX: 765-494-5896
Dept. of Botany & Plant Pathology E-mail: sherman-at-btny.purdue.edu
Purdue University
1057 Whistler Building
West Lafayette, IN 47907-1057

On Wednesday, September 13, 2000, Steve Chapman {PROTRAIN-at-CompuServe.COM} wrote:
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From daemon Wed Sep 13 14:01:33 2000



From: Malis, Tom :      malis-at-nrcan.gc.ca
Date: Wed, 13 Sep 2000 14:52:18 -0400
Subject: RE: serial sections

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"'Dorothy Roak Sorenson'"
{dsoren-at-umich.edu}
Cc: Microscopy-at-sparc5.microscopy.com


An elegant solution indeed, Dorothy (and others suggesting similar). Just
keep in mind the type of TEM for which the sections are headed, however.
For high-end TEM's in the physical sciences, where FEG sources, nanoprobes
and EELS analysis are quite common, the TEM specialists running the
instrument can be/should be very reluctant to allow specimens which might
outgas even a bit and gunk up the specimen chamber area. Your glue 'rim'
around the sections would do just that, I suspect.

Should anyone be doubtful of such effects, some years ago, Don Steele (Alcan
Aluminum in Kingston, Canada) did some neat serial sectioning of an Al-SiC
composite. (Really! Check the MSA '91 proceedings). I seem to recall that
Don had to collect the sections on formvar-coated girds (maybe even a
homemade formula?). In any event, when we tried to collect X-ray maps, the
film breakdown and redeposition piled up the contamination so fast as to
render mapping the Al and Si distribution very difficult. Any focused beam
analysis resulted in 'cones' on the section surface, rendering good EDX
analysis impossible. (People used to worry about column-borne contamination
in the early days of AEM. With the recent generations of instruments,
specimen-borne contamination is the issue). At least our case was of a very
localized phenomenon, the glue rim would result in much more widespread
'fallout'.

Finally, I always remind workshop attendees contemplating 'materials
science' ultramicrotomy, to be careful regarding C-analysis on embedded
materials, especially particulate. The e-beam (at least at the 120 keV of
our older scope) appears to instantly result in roughly a 20-30% mass loss
from any embedding resin, even at low beam intensities. This breakdown
produces a thin (~10 nm) film of C on the embedded bits which does not
interfere with imaging, but can be clearly seen in an EEL spectrum.

Tom Malis
Group Leader - Characterization
Materials Technology Laboratory
Natural Resources Canada (Govt. of Canada)
568 Booth St., Ottawa, Canada
ph. 613-992-2310
FAX 613-992-8735
email: malis-at-nrcan.gc.ca

----------
From: Dorothy Roak Sorenson [SMTP:dsoren-at-umich.edu]
Sent: Wednesday, September 13, 2000 8:52 AM
To: Mark West
Cc: Microscopy-at-sparc5.microscopy.com
Subject: Re: serial sections


------------------------------------------------------------------------
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America
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-----------------------------------------------------------------------.


Mark,

To glue serial sections together, make a gentle adhesive by
dissolving the
adhesive off of a piece of Scotch tape in chloroform. Paint the
upper and
lower block faces with your new adhesive, and, as you cut, the
sections
should stick together tenaciously.

Good luck!

Dotty Sorenson
Microscopy and Image Analysis Laboratory
Department of Cell and Developmental Biology
University if Michigan Medical School
Ann Arbor, Michigan
dsoren-at-umich.edu

On Tue, 12 Sep 2000, Mark West wrote:

}
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} The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
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ListServer-at-MSA.Microscopy.Com
} On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
}
-----------------------------------------------------------------------.
}
}
} Hi,
}
} I'm having (or trying to have) a go at doing serial ultrathin
sections of
} Epon-embedded material. I'd like to see a long unbroken ribbon
coming off
} the diamond knife, but after battling for a couple of hours
nothing seemed
} to work - the sections floated off happily on their own little
journeys
} and I wasn't going to chase them around. I tried cutting the
pyramid sides
} with a new Weckprep blade to get them as smooth as possible,
making them
} as parallel as railway tracks, lowering and raising the water
level in the
} trough, and turning off vibrating things in the lab (unfortunately
we're
} on the third floor), but nothing seemed to make any difference.
I'm going
} home now (smiling) but would like to hear of any tips and
experiences
} you've had that got you beautiful ribbons of sections (and any
other tips
} for serial sectioning).
}
} Looking forward to reading my mail tomorrow.
}
} Mark
}
}
}
}
}
}
} ********************************************
} Mark West,
} Unidad de Microscopia Electronica,
} (Electron Microscopy Unit)
} Instituto de Fisiologia Celular,
} Universidad Nacional Autonoma de Mexico,
} 04510 Mexico D.F.
}
} tel (unidad/lab) *(525) 622 5610*
} (casa/home) (525) 619 3020
} Fax (525) 616 2282
} ********************************************
}
}




From daemon Wed Sep 13 14:33:18 2000



From: Ranan Gulhan Aktas :      ranaoz-at-usa.net
Date: 13 Sep 00 13:23:21 MDT
Subject: Hildy Crowley, In memory....

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I just wanted to say I am so sorry to hear that. Her e-mails were always
excellent quides for me. She doesn't know me. However, I know her very well.
During the M&M meeting in Portland last year, I saw her for a minute. I was
willing to meet her. I just wanted to thank her and let her know that she was
also a wonderful teacher for me and for my friends, young electron
microscopists in Turkey. However, I could not get a chance to do that.
I was so surprised when I heard that she had retired a few months ago. Now, I
learn the reason.

Thank you very much all the way from Turkey, Hildy. I will really miss you.

Gulhan

Ranan Gulhan Aktas, M.D.
Trakya University
Faculty of Medicine
Pathology Department
Edirne 22030
TURKEY

Tel: +90 284 235 44 68
Fax: +90 284 235 76 52
e-mail: ranaoz-at-usa.net


____________________________________________________________________
Get free email and a permanent address at http://www.netaddress.com/?N=1



From daemon Wed Sep 13 14:52:50 2000



From: Robert Blystone :      rblyston-at-trinity.edu
Date: Wed, 13 Sep 2000 14:47:38 -0500
Subject: Preparation of serial sections for TEM

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To the List:

I ran across this in an obscure book on electron microscopy technique.

"To obtain quality serial sections for electron microscopy the following
procedure must be followed for consistent results.
1) Obtain one male goat.
2) Wait for new moon after a rain storm.
3) Obtain black robes which have been treated with a Z-stat gun.
4) Sacrifice goat in the light of the new moon.
5) Collect blood.
6) Spin down blood in centrifuge and collect goat plasma and store at 4 C
7) Take piece of plastic from sample block to be cut. Pulverize into
dust.
8) Mix plastic dust with goat plasma.
9) Inject plastic/goat into rabbit.
10) Wait one pay period.
11) Wait for full moon.
12) Place black robes on which have been retreated with a Z-stat gun.
13) Sacrifice rabbit which has been injected with plastic/goat antigen
under the full moon.
14) Collect antiplastic/goat antibody from rabbit.
15) Place one drop of antiplastic/goat antibody in the trough of diamond
knife.
16) Cut serial sections.
17) The antiplastic antisera should cause all the plastic sections to
arrange themselves in the proper order.
17) This should all make anti-sense."

Bob Blystone


--------------------------------
Robert V. Blystone, Ph.D.
rblyston-at-trinity.edu

Department of Biology
Trinity University
715 Stadium Drive
San Antonio, Texas 78212
210.999-7243 FAX 210/999-7229




From daemon Wed Sep 13 15:13:27 2000



From: Tamara Howard :      howard-at-cshl.org
Date: Wed, 13 Sep 2000 16:02:43 -0400 (EDT)
Subject: Re: Preparation of serial sections for TEM

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Wonderful! We have a similar protocol for FISHing particularly
recalcitrant mRNAs, but we use a chicken instead of a goat (not much space
here so we had to scale back to a smaller organism), and the blood goes in
the hybridization buffer.......

Tamara Howard
CSHL
(full moon tonight!)






From daemon Wed Sep 13 16:00:53 2000



From: Mark West :      mwest-at-ifcsun1.ifisiol.unam.mx
Date: Wed, 13 Sep 2000 15:53:16 -0600 (=?ISO-8859-1?Q?Hora_est=E1ndar_de_M=E9xico?=)
Subject: serial sections

Contents Retrieved from Microscopy Listserver Archives
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Hi,

Well, thanks for the flood of suggestions about getting a ribbon. I
trimmed the block sides with a glass knife (and enjoyed Mike Delannoy's
suggestion of 'trimming the top and bottom sides of your face with a glass
knife' - didn't know microscopy was such a painful job) and quite a decent
ribbon was the result. I tried the chloroform-glue idea and that seemed to
make the sections stick together more, but sometimes they gummed up a bit
on the knife. The next battle is picking up the ribbon on several slot
grids - I suspect that it's more practice than anything else. The method I
was shown was to go underneath the ribbon at an angle with the grid, raise
it until the waterline is on the top of the exposed formvar of the slot,
push the ribbon onto the waterline and draw the grid up at the same time,
and touch the ribbon with the hair at the point where the slot finishes to
break the ribbon, and remove the slot - all easier said than done! Any
ideas would be much appreciated.

Thanks,

Mark






********************************************
Mark West,
Unidad de Microscopia Electronica,
(Electron Microscopy Unit)
Instituto de Fisiologia Celular,
Universidad Nacional Autonoma de Mexico,
04510 Mexico D.F.

tel (unidad/lab) *(525) 622 5610*
(casa/home) (525) 619 3020
Fax (525) 616 2282
********************************************







From daemon Wed Sep 13 16:58:33 2000



From: Edward J. King :      king-at-biology.utah.edu
Date: Wed, 13 Sep 2000 15:50:14 -0600
Subject: SEM. Preparation of plant intervascular pit membranes.

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A user of our facility needs to prepare specimens to determine if
sub-micrometer pores are present in plant intervascular pit membranes.
The user is very concerned that critical point drying or, especially,
the dehydration steps prior to CPD, may introduce 'pores' that are
really artifacts. He is similarly concerned that freezing or
freeze-drying his specimens might also introduce 'pores' due to ice
crystal formation. Work he has already done suggests that these are
valid concerns.

Has anyone sufficient experience preparing pit membranes for SEM to
suggest a procedure we might follow to minimize the possibility of
creating small 'pores' where none may exist?

Thank you,

Ed King



From daemon Wed Sep 13 18:00:57 2000



From: Abdulrahman Zahrani :      abdulrahman_zahrani-at-yahoo.com
Date: Wed, 13 Sep 2000 14:11:00 -0700 (PDT)
Subject: Fluorescence Microscope

Contents Retrieved from Microscopy Listserver Archives
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Hi,

I am interested in knowing some details the
Flourescence Microscopes, their principles
and applications. Please e-mail me whatever details
you have available on the subject or the internet
address(s) of site(s) where I can obtain such
information.

Thanks,
Abdulrahman

__________________________________________________
Do You Yahoo!?
Yahoo! Mail - Free email you can access from anywhere!
http://mail.yahoo.com/



From daemon Wed Sep 13 18:42:01 2000



From: William A. Muller :      wamuller-at-med.cornell.edu
Date: Wed, 13 Sep 2000 19:31:41 -0400
Subject: position available

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Dear Colleagues:
I have made great contacts through this listserver in the past and hope
that I can again this time. I am in search of a postdoctoral fellow or
very independent technician to continue a program of research using modern
optical imaging methods to follow membrane trafficking in vascular
endothelial cells. It is a very exciting project that cuts across the
fields of cell biology, immunology, and pathology, and uses techniques from
standard transmission EM to tracking GFP-labeled proteins. If you have the
right background and are interested, or know of a colleague who fits these
criteria, please get in touch with me.
The following is the text of an ad that appeared in the August 10 issue of
Nature and the September issue of Nature Cell Biology:

Postdoctoral position in Membrane Trafficking
Postdoctoral position open to study membrane trafficking in
endothelial cells. The ideal candidate would have experience studying
regulated membrane trafficking at the cellular and molecular levels using
biochemical, microscopic, and molecular biology approaches. Experience with
modern imaging systems is critical. Experience with endothelial cells is a
definite "plus", but not necessary. The candidate should demonstrate
excellent communication skills in English, be able to work independently,
as well as to interact with a lively group of productive investigators
studying leukocyte-endothelial cell interactions. We are based at the
Weill Medical College of Cornell University in the nicest and safest area
of New York City. We interact extensively with investigators Weill as well
as at The Rockefeller University and Memorial Sloan-Kettering Cancer
Center, which are both just across the street. The position is
fully-funded and available immediately.

Submit CV and names (and contact numbers) of three references to:

William A. Muller, MD, PhD
Department of Pathology and Program in Immunology
Box 69
Weill Medical College of Cornell University
1300 York Avenue
New York, NY 10021
e-mail: wamuller-at-med.cornell.edu


Thank you very much for your help.

Sincerely,
Bill Muller


William A. Muller, MD, PhD
Associate Professor of Pathology
Weill Medical College of Cornell University
1300 York Avenue
New York, NY 10021

Phone: (212) 746-6487
Fax: (212) 746-6991
e-mail: wamuller-at-med.cornell.edu



From daemon Wed Sep 13 20:13:12 2000



From: Ekstrom, Harry :      harry.ekstrom-at-honeywell.com
Date: Wed, 13 Sep 2000 15:51:26 -0700
Subject: Carbon Evaporation Units

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We are attempting to put a ccd camera (high resolution, large area,
sensitivity = SO163) to replace films on 1200EX JEOL at a cost below $100k.
Would anyone be willing to share comments on recently acquired ccd camera
for TEM? Manufacturers welcome. Used ccds considered.


Marek Malecki, M.D., Ph.D.
Director and Principal Investigator

Molecular Imaging Laboratories
University of California at San Diego
address: 1500 Suite Bonner Hall, 9500 Gilman Drive, La Jolla, CA 92093-0368
phone - office: 8588223373
phone - lab: 8585342484
fax: 8588223715
pager: 8586161420
e.mail: mmm-at-ucsd.edu
e.pager: 1620024619-at-alphapage.airtouch.com
www site: http://mil.ucsd.edu/
ftp site: mil1.ucsd.edu







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At 11:42 AM 9/13/00 -0500, David Kerk wrote:
} We are closing down our EM facility. We have a Reichert Ultracut
} ultramicrotome available. Please make an offer, and be prepared to assume
} the cost of shipping to your facility.

I've got a AO / Reichert Ultracut microtome, too.
It's a model 701701. It's missing the 651102 control
panel, though, as well as the knife holder and object
holder. It looks just like the one at:
http://www.labx.com/v2/adsearch/Detail3.CFM?adnumb=54745

If I were to sell what I have, what price might it fetch?

- John



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We currently have a Bench Top Turbo carbon evaporation unit made by Denton.
We are very displeased with it. We consistently get non uniform coatings
that peel off of our metallographic mounts. Any suggestions on a
evaporation unit that works well or any information on 'sputtering" carbon?
Any other experiences out there?


Harry Ekstrom
Materials Laboratory

(602) 231-2744
e-mail: harry.ekstrom-at-honeywell.com




From daemon Thu Sep 14 01:58:19 2000



From: Marilyn Henderson :      marilyn.henderson-at-adelaide.edu.au
Date: Thu, 14 Sep 2000 16:16:53 +0930
Subject: Serial Section pick-up

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Dear Mark,
With coated slot grids it's easier to come down on top of your
ribbon
of sections. Break up your ribbon into strips which will fit over your
slot first. Then hold your coated slot grid over the sections (you can
see the ribbon of sections through your coating and then slowly descend
onto your stip of sections. The sections adhere immediately with the
first touch. This method avoids any surface tension problems between the
grid and water surface which can send the grids flying away from your
grid.

Good Luck.
Marilyn

Mark West wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hi,
}
} Well, thanks for the flood of suggestions about getting a ribbon. I
} trimmed the block sides with a glass knife (and enjoyed Mike Delannoy's
} suggestion of 'trimming the top and bottom sides of your face with a glass
} knife' - didn't know microscopy was such a painful job) and quite a decent
} ribbon was the result. I tried the chloroform-glue idea and that seemed to
} make the sections stick together more, but sometimes they gummed up a bit
} on the knife. The next battle is picking up the ribbon on several slot
} grids - I suspect that it's more practice than anything else. The method I
} was shown was to go underneath the ribbon at an angle with the grid, raise
} it until the waterline is on the top of the exposed formvar of the slot,
} push the ribbon onto the waterline and draw the grid up at the same time,
} and touch the ribbon with the hair at the point where the slot finishes to
} break the ribbon, and remove the slot - all easier said than done! Any
} ideas would be much appreciated.
}
} Thanks,
}
} Mark
}
} ********************************************
} Mark West,
} Unidad de Microscopia Electronica,
} (Electron Microscopy Unit)
} Instituto de Fisiologia Celular,
} Universidad Nacional Autonoma de Mexico,
} 04510 Mexico D.F.
}
} tel (unidad/lab) *(525) 622 5610*
} (casa/home) (525) 619 3020
} Fax (525) 616 2282
} ********************************************



From daemon Thu Sep 14 02:33:04 2000



From: Alexander Mironov :      amironov-at-nki.nl
Date: Thu, 14 Sep 2000 10:27:07 +0200
Subject: Re: serial sections

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Mark West wrote:

} The next battle is picking up the ribbon on several slot
} grids - I suspect that it's more practice than anything else.

Mark,

I got rather consistent results with Perfect Loop from EMS. They even describe
how to pick up sections (http://www.emsdiasum.com/ems/grids/accessories.html).
Nevertheless I used it in slightly different way. After step 3 I put the loop
with sections in the holder (stand) in a way that I can see it looking in
microtome oculars. Then I put the slot grid coated with formvar just above the
loop in desired orientation and slowly lower it down until section attach to
the formvar film. Then I moved the grid with attached sections to side (not
up!) so that practically all water remains in the loop. Result: I got serial
sections on the grid with desired orientation and without necessity to remove
water (a lot of dust can go from filter papaer to the grid when you try to dry
it).

I have no any interest in EMS.

Sincerely,
--
Alexander Mironov Jr.
Division of Tumor Biology, H4
The Netherlands Cancer Institute
Plesmanlaan 121
1066 CX Amsterdam

Tel. +31-(0)20-512-2017
Fax +31-(0)20-512-2029
E-mail: amironov-at-nki.nl





From daemon Thu Sep 14 02:46:44 2000



From: Ron Doole :      ron.doole-at-materials.oxford.ac.uk
Date: Thu, 14 Sep 2000 08:43:31 +0100 (GMT Daylight Time)
Subject: Re: LN2 and Contamination

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Hi Debby,

Most modern TEMs are ion pumped (or turbo pumped) to
prevent the diffision pump oil backstreaming problem. The
diffusion pump is used when initially evacuating the column
or gun, pumping the camera and viewing chamber and when
warming up the specimen cold trap at the end of sessions.
Whereas it would be good to have a N2 trap on the diffusion
pump when it does pump the column the water vapour from the
camera and viewing chamber (due to the films) would stall
the backing pump when the N2 trap warmed up.
Prior to ion pumped columns we did use a N2 trap on the TEM
column diffusion pump (circa 1980).
Ion pumped or turbo molecular pumped SEMs are also
available.

Regards,
Ron


On 13 Sep 2000 13:00:11 -0500 Debby Sherman
{sherman-at-btny.purdue.edu} wrote:

}
------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy
Society of America } To Subscribe/Unsubscribe -- Send
Email to ListServer-at-MSA.Microscopy.Com } On-Line Help
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}
-----------------------------------------------------------------------.
}
} } Steve,
} Thanks for this post. I try to get this through to
our users and we faithfully use and require use of N2 on
all scopes. However, there are other facilities on campus
who do not use N2 or use it intermittantly. When this topic
comes up again, I intend to hand them your post and ask
them to determine contamination rates on their instruments
to convince them of the necessity of using N2. } One
question that I have is that our main nitrogen trap on the
SEM is above the diffusion pump to minimize backstreaming
from the pump into the specimen chamber. (We also have a
decontaminator close to the specimen that is cooled when we
do cryo-SEM.) Now this is no doubt more of a problem with
SEM than TEM due to the location of the diffusion pump.
However, I wonder why most TEM manufacturers do not also
use a nitrogen trap in this location, at least in the
higher end scopes, in addition to the decontaminator in the
specimen area. It would seem that, since contamination
rate in the TEM is likely to be more critical to high
resolution, it would also be used to help maintain the best
possible vacuum in the column. } I am aware that the
majority of the contamination is produced in the TEM by
interaction of the beam with the specimen thus the need for
decontaminator close to the specimen. However, my
understanding is that the better the vacuum, the fewer the
interactions of the beam electrons with molecules in the
column and the more coherent the beam....i.e. better
potential resolution. } Debby
} } Debby Sherman Phone: 765-494-6666
} Microscopy Center in Agriculture FAX: 765-494-5896 }
Dept. of Botany & Plant Pathology E-mail:
sherman-at-btny.purdue.edu } Purdue University

} 1057 Whistler Building
} West Lafayette, IN 47907-1057 }
} On Wednesday, September 13, 2000, Steve Chapman
{PROTRAIN-at-CompuServe.COM} wrote: }
} ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy
Society of America } } To Subscribe/Unsubscribe -- Send
Email to ListServer-at-MSA.Microscopy.Com } } On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
}
} -----------------------------------------------------------------------.
} }
} } } } Hi
} } } } I have read the offerings with interest and would
like to make a few } } comments.
} } } } Firstly - when a manufacturer designs an instrument
they do not fit cold } } traps for fun, they believe that to
obtain the best performance from their } } instrument you
need the facilities that they fit. I have worked with }
} design teams at two manufacturers and this is the attitude
that they both } } took!
} } } } Secondly - I have in the past, in countries where
LN2 was not available, } } used dry ice (Solid CO2) and
acetone in LN2 traps. I believe this brings } } the
temperature down to -80deg C and it works quite well much
better than } } no cooling at all.
} } } } Thirdly there are imaging techniques that allow us
to measure the } } contamination rate. Place a holey film
in the TEM and without LN2 in use } } and take a high
resolution picture of a hole. Leave the beam on the }
} specimen and repeat the picture on the same hole 20
minutes later (that is } } if you still have a hole).
Measure the two hole widths, take the smaller } } from the
larger and divide by 2 (2 contaminated edges) divide by the
time } } between the two pictures and divide by the
magnification. You now have } } your contamination rate in
nm/minute. Then try this with the LN2 systems } } in action
and you will see just how well the LN2 systems work. } }
} } The same system can be used with a SEM but use a well
coated latex sphere } } in place of the hole. In this case
the sphere will grow with time as the } } contamination
builds round the sphere. } }
} } Many years ago (1968) Hitachi produced a paper on LN2
systems. They gist } } was that if you used LN2 systems for
a period of time and then stopped, it } } took an equal
period of time before the vacuum level and contamination
rate } } fell back to the original level. Of course you
could not count the time if } } you let the column to air.
} } } } A few more things to think about and a way of
proving just how clean your } } microscope is.
} } } } Steve Chapman
} } Senior Consultant Protrain } } For consultancy and
training by professional World Wide } } Tel +44 1280 814774
Fax +44 1280 814007 } } www.emcourses.com
} } } }
} }

----------------------
Mr. R.C. Doole Department of Materials,
University of Oxford.
Parks Road, Oxford. OX1 3PH. UK.
Phone +44 (0) 1865 273701
Fax +44 (0) 1865 283333
ron.doole-at-materials.ox.ac.uk




From daemon Thu Sep 14 02:46:54 2000



From: Witold Zielinski :      WIZIEL-at-inmat.pw.edu.pl
Date: Thu, 14 Sep 2000 09:43:50 +0000
Subject: Re: LN2 and Contamination

Contents Retrieved from Microscopy Listserver Archives
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*Date sent: Wed, 13 Sep 2000 04:34:23 -0400
*From: Steve Chapman {PROTRAIN-at-CompuServe.COM}
*Subject: LN2 and Contamination
*To: American Soc {Microscopy-at-sparc5.microscopy.com}

*------------------------------------------------------------------------
*The Microscopy ListServer -- Sponsor: The Microscopy Society of America

I understand the procedure regarding the TEM, however could you
explain to what I should compare the contamination rate in the SEM.

Best regards,

Witold


*
*Hi
*
*I have read the offerings with interest and would like to make a few
*comments.
*
*Firstly - when a manufacturer designs an instrument they do not fit cold
*traps for fun, they believe that to obtain the best performance from their
*instrument you need the facilities that they fit. I have worked with
*design teams at two manufacturers and this is the attitude that they both
*took!
*
*Secondly - I have in the past, in countries where LN2 was not available,
*used dry ice (Solid CO2) and acetone in LN2 traps. I believe this brings
*the temperature down to -80deg C and it works quite well much better than
*no cooling at all.
*
*Thirdly there are imaging techniques that allow us to measure the
*contamination rate. Place a holey film in the TEM and without LN2 in use
*and take a high resolution picture of a hole. Leave the beam on the
*specimen and repeat the picture on the same hole 20 minutes later (that is
*if you still have a hole). Measure the two hole widths, take the smaller
*from the larger and divide by 2 (2 contaminated edges) divide by the time
*between the two pictures and divide by the magnification. You now have
*your contamination rate in nm/minute. Then try this with the LN2 systems
*in action and you will see just how well the LN2 systems work.
*
*The same system can be used with a SEM but use a well coated latex sphere
*in place of the hole. In this case the sphere will grow with time as the
*contamination builds round the sphere.
*
*Many years ago (1968) Hitachi produced a paper on LN2 systems. They gist
*was that if you used LN2 systems for a period of time and then stopped, it
*took an equal period of time before the vacuum level and contamination rate
*fell back to the original level. Of course you could not count the time if
*you let the column to air.
*
*A few more things to think about and a way of proving just how clean your
*microscope is.
*
*Steve Chapman
*Senior Consultant Protrain
*For consultancy and training by professional World Wide
*Tel +44 1280 814774 Fax +44 1280 814007
*www.emcourses.com
*
*
%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
Witold Zielinski, Ph.D.
Warsaw University of Technology
Department of Materials Science and Engineering
02-507 Warszawa, Woloska 141
POLAND

phone #: /48 22/ 660 84 41
fax #: /48 22/ 848 48 75



From daemon Thu Sep 14 02:53:45 2000



From: Keith Ryan :      kpr-at-pml.ac.uk
Date: Thu, 14 Sep 2000 08:45:02 +0100
Subject: Re: serial sections - collection of...

Contents Retrieved from Microscopy Listserver Archives
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This may help? I have used a (no doubt frowned on) practice for many
years for easy collection of sections:

Make up 0.2% sodium lauryl sulphate (a detergent) in distilled water.
Grids are dipped in this just prior to collecting sections, then
drained on filter paper before introducing under the water in the
knife boat and bringing up under the sections. The sections can be
manipulated along filmed slots with an eyelash before the residual
fillm of water dries. You can play this game under the binocular for
some minutes while watching the water evaporate. Its a bit like
watching paint dry!

Sometimes, raising the water meniscus (i.e. to convex) is useful when
trying to position sections prior to collection.

Too much carry-over of detergent can cause the sections to run
together and bunch up (a surface tension effect of the detergent).

The main problem with this practice is that you can only do this
after cutting a batch of sections. If you wish to continue cutting
then it is adviseable to flush the boat with fresh water, otherwise
the block face will probably "wet" if you resume cutting. I normally
pipette out the water and replace it three times - that works.

Keith Ryan
Marine Biological Association
Plymouth, UK



From daemon Thu Sep 14 03:15:07 2000



From: jim :      jim-at-proscitech.com.au
Date: Thu, 14 Sep 2000 16:01:53 +1000
Subject: RE: Alternatives to Critical point drying

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Dear Ed,

Unfortunately our CPD is out of service and my bottle of PELDRI
nearly empty, therefore I am forever looking for new, fast,
inexpensive ways to prepare my samples.

Although I do not work with plant material I was very interested in a
publication that describes the use of glycerol or triethylene glycol
to infiltrate biological specimens (mostly plant material) so that
they may be observed in the SEM.

("Liquid substitution: a versatile procedure for SEM specimen
preparation of biological materials without drying or coating" Journal
of Microscopy, Vol. 172, 3. 1993 pp195-203. Ensikat H.j. &
Barthlott, W.)

.."The main objectives of these preparation methods are to
stabilize the specimen, to prevent shrinkage and other artefacts
during dehydration, and to render the sample electrically
conductive....specimen are not dried, but their water is instead
substituted for a liquid..."

If the researcher tries the method I would be very interested to hear
how it worked.

Regards

Claudia





On 13 Sep 2000, at 15:50, Edward J. King wrote:

Date sent: Wed, 13 Sep 2000 15:50:14 -0600
} From: "Edward J. King" {king-at-biology.utah.edu}
To: Microscopy Listserv {Microscopy-at-sparc5.microscopy.com}


I have no experience of looking at pits. Another
possiblility to reduce artifacts would be to use an ESEM.
I think it might be difficult to get the resolution on
unfixed material with a tungsten instrument. A field
emission ESEM would have the resolution at lower voltages
but may still require a comparison of fixed and unfixed
material. Unfixed material has to be handled carefully to
avoid beam damage artifacts.

Dave



On Wed, 13 Sep 2000 15:50:14 -0600 "Edward J. King"
{king-at-biology.utah.edu} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} A user of our facility needs to prepare specimens to determine if
} sub-micrometer pores are present in plant intervascular pit membranes.
} The user is very concerned that critical point drying or, especially,
} the dehydration steps prior to CPD, may introduce 'pores' that are
} really artifacts. He is similarly concerned that freezing or
} freeze-drying his specimens might also introduce 'pores' due to ice
} crystal formation. Work he has already done suggests that these are
} valid concerns.
}
} Has anyone sufficient experience preparing pit membranes for SEM to
} suggest a procedure we might follow to minimize the possibility of
} creating small 'pores' where none may exist?
}
} Thank you,
}
} Ed King
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"



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Very satisfactory results have been reported for pollen (see ref.
below) but I have found it worthless on plant cells in tissue culture,
for which cryo-SEM must be the way to go. HMDS may work
better on densely cytoplasmic material than on very vacuolate plant
tissue.

I would be interested to know what the principles of operation of
HMDS are thought to be. Does anyone have specific information
about this?

Chris

Chissoe,W.F.; Vezey,E.L.; Skvarla,J.J. (1994)
Hexamethyldisilazane as a drying agent for pollen scanning
electron- microscopy. Biotechnic & Histochemistry69, 192-198
Abstract:
Use of hexamethyldisilazane (HMDS) as a final dehydrating
solution provides robust, undistorted secondary electron images of
a variety of angiosperm and gymnosperm pollen grains, including
those considered to be susceptible to collapse in the scanning
electron microscope. Ease of handling, low cost, lack of
specialized equipment, minimal expenditure of time, and high rate
of success are factors that favor HMDS over other drying agents for
preparing pollen grains for scanning electron microscopy


} From: "Patton, David" {David.Patton-at-uwe.ac.uk}
To: Tobias Baskin {BaskinT-at-missouri.edu}
Copies to: Microscopy-at-sparc5.microscopy.com


I confused myself with acronyms and broadcast the result! The HMDS may be used
for drying specimens for SEM as noted. I also ventured that it can be used for
dehydration - wrong. For rapid, complete dehydration dimethoxypropane (DMP) is
used; it converts residual water to acetone.
Thanks to Tobias for spotting that. No doubt a thousand others noted the error
but were too polite (?) to write.
I think DMP works only well to remove residual water after prior dehydration.
Cheers
Jim Darley
ProSciTech

On Wednesday, September 13, 2000 11:43 PM, Tobias Baskin
[SMTP:BaskinT-at-missouri.edu] wrote:
} Jim,
} Thanks for the info. How does HMDS compare with
} dimethoxypropane as a water scavenger? I use a few % of latter in
} freeze substitution brews as a "cemical sieve". I have heard to folks
} using DMP as one shot dehydration before embedding. I even tried it.
} Worthless for our stuff. Just curious about the HMDS.
}
} Tobias
}
} } In my previous response I gave the method, though I've used Chloroform. HMDS
} } may have a theoretical advantage, but I expect that the results would be
} } identical.
} } HMDS was introduced for rapid processing, particularly fast and complete
} } dehydration. It chemically interacts with water and forms acetone. When at
} } least the last dehydration is in HMDS, the user can be sure that
} } dehydration is
} } excellent even with doubtful ethanol or acetone.
} }
} } During the air drying process residual water and solvent molecules exerts
} } huge
} } pressures onto membranes and these cause shrinkage and shriveling of any
} } specimen.
} } The drying mechanism:
} } Using Peldri, drying only occurred at the Peldri/ air interface, but the
} } specimen is held rigid in the solid Peldri material. As the Peldri slowly
} } sublimes, more of the specimen is dried.
} } During solvent drying the specimen is in a solvent saturated atmosphere and
} } drying is very slow - over 48 hours. In this method there is little pressure
} } exerted by molecules trying to pass through membranes and consequently
} } shrinkage is minimized.
} } Its a good alternative method and sometimes works very well. Given a choice
} } I'll try CPD first.
} } Cheers
} } Jim Darley
} } ProSciTech Microscopy PLUS
} } PO Box 111, Thuringowa QLD 4817 Australia
} } Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
} } Great microscopy catalogue, 500 Links, MSDS, User Notes
} } ABN: 99 724 136 560 www.proscitech.com
} }
} } On Wednesday, September 13, 2000 12:21 AM, Tobias Baskin
} } [SMTP:BaskinT-at-missouri.edu] wrote:
} } }
} } }
} } } Greetings,
} } } Several replies have mentioned this mysterious solvent for
} } } CPD, and that it works on some type of specimens. Could someone
} } } outline how this is used (infiltrate and then evap to air, or what?)
} } } and if there is any rhyme or reason about what kinds of specimens
} } } that work???
} } }
} } } THanks,
} } } Tobias
} } }
} } } }
} } } }
} } } } We use HMDS (Hexamethyldisilazane) from Sigma.
} } } }
} } } } Dave
} } } }
} } } }
} } } } On Tue, 12 Sep 2000 08:53:03 -0500 Cameron Hind
} } } } {hindc-at-baxter.com} wrote:
} } } }
} } } } }
} } } } }
------------------------------------------------------------------------
} } } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} } } } } America
} } } } } To Subscribe/Unsubscribe -- Send Email to
} } } } } ListServer-at-MSA.Microscopy.Com
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} } } } }
} } } } }
-----------------------------------------------------------------------.
} } } } }
} } } } }
} } } } }
} } } } } Hi Listservers,
} } } } }
} } } } } I have a memory lapse-can you remind me of the reagent used as an
} } } } } alternative to CPD for SEM;or do you know of/recommend any
} } } safe (not too
} } } } } toxic) alternative method to CPD?-any reply would be appreciated
} } } } }
} } } } } Cameron
} } } } }
} } } } } Mr. Cameron Hind
} } } } } Research Scientist
} } } } } Advanced Technologies group
} } } } } Baxter R & D Europe S.C.R.L.
} } } } } Rue du ProgrEs 7
} } } } } 1400 Nivelles
} } } } } Belgium
} } } } }
} } } } } Tel:+32 67 882 511
} } } } } Fax:+32 67 217 191
} } } } } e-mail: hindc-at-baxter.com
} } } } }
} } } } }
} } } } }
} } } } }
} } } } }
} } } } }
} } } } }
} } } } }
} } } } }
} } } } }
} } } } }
} } } } }
} } } } }
} } } } }
} } } } }
} } } } }
} } } } }
} } } } }
} } } } }
} } } } }
} } } }
} } } } ----------------------------------------
} } } } Patton, David
} } } } Email: David.Patton-at-uwe.ac.uk
} } } } "University of the West of England"
} } }
} } } --
} } } _ ____ __ ____ Tobias I. Baskin
} } } / \ / / \ / \ \ University
} } } of Missouri
} } } / | / / \ \ \ Biological
} } } Sciences
} } } /_ / __ /__ \ \ \__ 109 Tucker Hall
} } } / / / \ \ \
} } } Columbia, MO 65211-7400 USA
} } } / / / \ \ \ voice:
} } } 573-882-0173
} } } / /____ / \ \__/ \____ fax: 573-882-0123
} } }
} } }
}
} --
} _ ____ __ ____ Tobias I. Baskin
} / \ / / \ / \ \ University of Missouri
} / | / / \ \ \ Biological
} Sciences
} /_ / __ /__ \ \ \__ 109 Tucker Hall
} / / / \ \ \
} Columbia, MO 65211-7400 USA
} / / / \ \ \ voice:
} 573-882-0173
} / /____ / \ \__/ \____ fax: 573-882-0123



From daemon Thu Sep 14 16:35:04 2000



From: jfb :      jfb-at-uidaho.edu
Date: Thu, 14 Sep 2000 13:23:29 -0700
Subject: Re: Alternatives to Critical point drying

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Jim,
I'm not sure if it is the same, but I have used 2,2-dimethoxypropene as
an intermediate agent between 100% ethanol and air drying of the
sample. It works very well and there is little distortion of the
surface morphology.

Franklin Bailey
Electron Microscopy Center
University of Idaho
Moscow, ID 83844-2204




From daemon Thu Sep 14 16:35:04 2000



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Fri, 15 Sep 2000 08:55:15 GMT+1200
Subject: Re: Carbon Evaporation Units

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



}
} We currently have a Bench Top Turbo carbon evaporation unit made by Denton.
} We are very displeased with it. We consistently get non uniform coatings
} that peel off of our metallographic mounts. Any suggestions on a
} evaporation unit that works well or any information on 'sputtering" carbon?
} Any other experiences out there?
}

I would look very carefully at the cleanliness of the samples before
coating.
I use methanol then Freon then a dry Kimwipe, and the carbon
coating is tenacious on brass.

cheers

rtch


Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand



From daemon Thu Sep 14 16:35:04 2000



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Fri, 15 Sep 2000 08:51:01 GMT+1200
Subject: Re: LN2 and Contamination

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hi, Ron and all

Your first sentence is interesting, if I may stray a bit from the
actual topic.
I had formed the idea that the back-streamed oil is not diff pump
fluid, but rotary pump oil.
But if the phenomenon disappears when a turbo or ion pump replaces
the diff pump, then that kindof exonerates the rotary pump, doesn't
it?
Or is it just that the turbo or ion pump is better than the diff pump
at preventing rotary pump vapour from diffusing backwards through it?
Anybody know the definitive answer to this?




}
} Most modern TEMs are ion pumped (or turbo pumped) to
} prevent the diffision pump oil backstreaming problem. The
} diffusion pump is used when initially evacuating the column
} or gun, pumping the camera and viewing chamber and when
} warming up the specimen cold trap at the end of sessions.
} Whereas it would be good to have a N2 trap on the diffusion
} pump when it does pump the column the water vapour from the
} camera and viewing chamber (due to the films) would stall
} the backing pump when the N2 trap warmed up.
} Prior to ion pumped columns we did use a N2 trap on the TEM
} column diffusion pump (circa 1980).
} Ion pumped or turbo molecular pumped SEMs are also
} available.
}
} Regards,
} Ron
}


cheers

rtch

Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand



From daemon Thu Sep 14 18:44:37 2000



From: Rosemary White :      Rosemary.White-at-pi.csiro.au
Date: Fri, 15 Sep 2000 10:36:14 +1000
Subject: SEM. Preparation of plant intervascular pit membranes.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Ed,

There's a recent paper in Annals of Botany on exactly this topic, by Shane,
McCully and Canny - earlier this year, April?

cheers,
Rosemary


Rosemary White
Microscopy Centre
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia

phone 61-2-6246 5475
fax 61-2-6246 5000
email r.white-at-pi.csiro.au





From daemon Thu Sep 14 20:17:36 2000



From: Gregory M Fahy :      gregoryfahy-at-juno.com
Date: Thu, 14 Sep 2000 09:18:19 -0700
Subject: Shipping cells at -20oC

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In response to Tamara Howard's predicament, here is
an idea to consider. One can purchase bags of phase
change material from Polar Tech Industries, Inc.,
in Genoa, IL (60135) that can be placed into a -20oC
freezer and allowed to freeze. These can be placed into
a styrofoam dry ice shipping box (commercially available
from VWR with an external cardboard box for shipping)
and should hold your samples between -20 and 0oC during
shipment. This would be your ideal temperature range
for shipment, and the temperature will hold for the duration
of the air flight if your box size is reasonable. The foam
refrigerant material stays in the bags, so there is no mess
or hazard.

For more details, contact Polar Tech at 1-800-ICE-BRIX,
or 815-784-9000, or at www.polar-tech.com.

I'd like to know how this works, if you haven't already opted
for another approach.

Greg Fahy, Ph.D.
21st Century Medicine

________________________________________________________________
YOU'RE PAYING TOO MUCH FOR THE INTERNET!
Juno now offers FREE Internet Access!
Try it today - there's no risk! For your FREE software, visit:
http://dl.www.juno.com/get/tagj.



From daemon Thu Sep 14 20:23:50 2000



From: Martin Izquierdo :      MartinI-at-oztek.com
Date: Thu, 14 Sep 2000 18:13:30 -0700
Subject: Fourier Transform Infrared Spectroscopy (FTIR)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does anyone have any information on Fourier Transform Infrared (FTIR)
Spectroscopy used for organic contamination analysis? Specifically
manufacturers and the appx. price for the equipment .
Any information would be helpful.

Martin A. Izquierdo
Analytical Laboratory
Cerprobe, Inc.




From daemon Thu Sep 14 21:04:58 2000



From: jim :      jim-at-proscitech.com.au
Date: Fri, 15 Sep 2000 11:52:57 +1000
Subject: RE: Carbon Evaporation Units

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Retch is right, but you could also have a poor cooling system on that pump, or
just a badly designed system. In either case backstreaming of oil vapour would
lead to poorly adhearing films.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Friday, September 15, 2000 6:55 PM, Retch Sims [SMTP:r.sims-at-auckland.ac.nz]
wrote:
}
}
}
} }
} } We currently have a Bench Top Turbo carbon evaporation unit made by Denton.
} } We are very displeased with it. We consistently get non uniform coatings
} } that peel off of our metallographic mounts. Any suggestions on a
} } evaporation unit that works well or any information on 'sputtering" carbon?
} } Any other experiences out there?
} }
}
} I would look very carefully at the cleanliness of the samples before
} coating.
} I use methanol then Freon then a dry Kimwipe, and the carbon
} coating is tenacious on brass.
}
} cheers
}
} rtch
}
}
} Ritchie Sims Phone : 64 9 3737599 ext 7713
} Department of Geology Fax : 64 9 3737435
} The University of Auckland email : r.sims-at-auckland.ac.nz
} Private Bag 92019
} Auckland
} New Zealand




From daemon Thu Sep 14 22:02:41 2000



From: =?ks_c_5601-1987?B?Sm9uZG8gWXVuIMCxwbi1tQ==?= :      jdyun-at-kyungnam.ac.kr
Date: Fri, 15 Sep 2000 11:53:34 +0900
Subject: Dye Sublimation Printer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear netters:

I plan to buy CCD camera for TEM and want to remove wet dark room.
So I need good printer. I think dye sublimation printer is the best choice. But I do not know what model is the best.
Is there anybody who uses dye-sub printer in EM lab? Can you suggest what model is the best? My budget is about 5000 US$ or a little more.
Thank you in advance.

Best regards,

Jondo Yun
Kyungnam University
Division of Advanced Materials
Electron Microscopy Laboratory
449 Weolyeong-dong
Masan, 631-701, Korea

(tel) 82-55-249-2697 (office)
82-55-249-2564 (EM lab)
82-55-249-2719 (Lab)
(fax)82-55-248-5033 (div. office)
(email) jdyun-at-hanma.kyungnam.ac.kr





From daemon Fri Sep 15 03:35:36 2000



From: A.Walker :      Alan.Walker-at-sheffield.ac.uk
Date: Fri, 15 Sep 2000 10:16:12 +0100
Subject: printers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Jondo
Depends what the end-use of your prints is going to be. The most
recent 6-colour + black 1440 dpi Epson Stylus printers produce
absolutely stunning A4 size prints for a lot less capital and
consumables cost. At arm's length you cannot really tell the
difference between the Epson inkjet prints and a dye-sub print.
With your budget you could buy a couple of these printers and still
have enough left to buy a top-spec laptop! Epson also produce
professional spec. inkjet printers, such as the Pro 5000 which are
much more expensive, but may just be within your budget.
see http://www.tssphoto.com/sp/dg/news/dot_comp.html for more
details and links.

However, if you are determined to buy a dye-sub type printer,
check out the Fuji Pictrography printers which combine laser
exposed silver halide technology with dye transfer. The results are
amazing.
Chris

Send reply to: "Jondo Yun À±Á¸µµ" {jdyun-at-kyungnam.ac.kr}
} From: "Jondo Yun À±Á¸µµ" {jdyun-at-kyungnam.ac.kr}
To: "MicroscopyListserver" {Microscopy-at-sparc5.microscopy.com}


Hi Jondo,

I have no experience of dye sublimation printers but feel I must
tell you how good our Epson Stylus Photo 1270 is. Using, admittedly
expensive, photo quality paper we find it beats our very expensive
colour laser printer for image quality. Unless you want dozens of
copies done very quickly, I would certainly give the Epson a trial -
and it costs a fraction of your budget, the remainder of
which could buy a lot of consumables.
BTW, I do not work for Epson

Kind regards,

Alan

*********************************************
Alan Walker
Dept of Electronic and Electrical Engineering
University of Sheffield
Mappin Street
Sheffield S1 3JD
United Kingdom

Tel:+44-(0)114-2225365
Fax:+44-(0)114-2726391
alan.walker-at-shef.ac.uk
http://borg.shef.ac.uk/fegtem/index.htm
*********************************************



From daemon Fri Sep 15 04:38:42 2000



From: Steve Chapman :      PROTRAIN-at-CompuServe.COM
Date: Fri, 15 Sep 2000 05:28:39 -0400
Subject: LN2 and Contamination

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Debby,

Glad I was able to help. I now number the "we do not use LN2" excuses 1 to
10 and simply ask which a client wishes to use as there is not such a thing
as a "good excuse", I have heard them all!

Ron has given you a good explanation of DP traps, uses and problems but we
should discuss pumping speed and TEM contamination a little further.

A vacuum in a TEM has fast and slow pumping areas. The electron gun is a
fast pumping area but small pipes and pole piece apertures restrict gas
flow and produce slow pumping areas, the specimen area in a TEM for
example! No matter how good the vacuum may be at the DP it is only by
improving the vacuum right by the TEM specimen that we see dramatic
improvements, cold traps and cold fingers right by the specimen are the
best route to lowering contamination.

Contamination in the TEM deposits on the top and the bottom of the specimen
thickening the material and softening the image as the structures are
gradually masked. Thicker specimens mean poorer resolution, often related
as resolution is equal to one tenth of the specimen thickness. Improve
the vacuum and you improve the contamination rate and an area that people
do not relate to, you improve the gun vacuum and high voltage stability!
So a better vacuum gives us a better contamination rate and better high
voltage stability these two together will improve resolution.

I note you use a cold finger in the SEM when carrying out cryo experiments,
I try to use such a device as much as possible if any high resolution study
is taking place. Contamination rate tests would probably indicate as much
as a five fold improvement when a trap is within inches of the specimen. I
have designed several circular traps to fit round the pole piece of FEG
SEMs, here contamination becomes the biggest barrier to very high
resolution.

Good luck with those who have not taken to the LN2 gospel, keep at it!

Steve Chapman
Senior Consultant Protrain
For professional training and consultancy in EM world wide
Tel +44 1280 814774 Fax +44 1280 814007
www.emcourses.com



From daemon Fri Sep 15 07:03:32 2000



From: Alan Stone :      as-at-astonmet.com
Date: Fri, 15 Sep 2000 06:51:53 -0500
Subject: Need ISI SX-30 help for high school

Contents Retrieved from Microscopy Listserver Archives
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Dear Group,

I am trying to help a local high school set up an ISI SX-30 Super III SEM.
It had been sitting for several years before being moved to the school. I
am unfamiliar with this scope and we are trying to do some troubleshooting.
We would greatly appreciate some help.

When the [Operate] button is depressed, it should pump down the
chamber/column. However, it seems as though a valve is opened to atmosphere
and loads the rotary pump. We hear hissing below the chamber which seems to
be coming from a nozzle attached to a solenoid. We are wondering if there
should be a hose attached to the nozzle and where it might go. Or is this
an electrical problem?

Also, there is a loose snap wire connector in the back above the diffusion
pump. It is marked JKA6 and contains a red and a yellow wire. I could not
see the wires went to.

If anyone is familiar with this instrument, we would greatly appreciate
some help in getting us started. The instrument was donated to the school
and there are limited funds for bringing in service engineers.

Thanks,







Alan Stone
ASTON Metallurgical Services



From daemon Fri Sep 15 07:47:33 2000



From: David_Bell-at-millipore.com
Date: Fri, 15 Sep 2000 08:38:55 -0400
Subject: Re: Fourier Transform Infrared Spectroscopy (FTIR)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hello Martin,

Our FTIR expert here likes the complete system by Nicolet, but also said
Perkin-Elmer and Biorad are a couple of other companies that make nice
systems. I'm sure I'm leaving some out, as I'm not an FTIR expert, so
forgive me if your company isn't on the list! As far as pricing, he
mentioned a complete system goes for about $120,00US with only one lens.
You can expect to add about $15,000US for each attachment, such as ATR and
micro-ATR.

Again, I'm sure some vendors can give you more pertinent information, but
this should put you in the ball park.

Hope this helps,



David Bell
Scientist
Electron Microscopy Lab
Millipore Corporation
80 Ashby Road
Bedford, MA 01730
(781) 533-2108





Martin
Izquierdo To: "'Microscopy-at-MSA.Microscopy.Com'"
{MartinI-at-ozte {Microscopy-at-sparc5.microscopy.com}
k.com} cc:
Subject: Fourier Transform Infrared Spectroscopy (FTIR)
09/14/00
09:13 PM






------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Does anyone have any information on Fourier Transform Infrared (FTIR)
Spectroscopy used for organic contamination analysis? Specifically
manufacturers and the appx. price for the equipment .
Any information would be helpful.

Martin A. Izquierdo
Analytical Laboratory
Cerprobe, Inc.








From daemon Fri Sep 15 08:10:59 2000



From: Ni, Chao-Ying :      CYNi-at-rodel.com
Date: Fri, 15 Sep 2000 08:55:40 -0400
Subject: RE: Carbon Evaporation Units

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Harry,

To the best of my knowledge, there is no such thing as "sputtering carbon".
But I would suggest to look into your carbon source. Try different vendors.
Also, make sure your vacuum is good.

Have a nice day!

Chao-Ying Ni
Rodel Inc.
451 Bellevue Road
Newark, DE 19713
(302) 366-0500 ext 2812


-----Original Message-----
} From: jim [mailto:jim-at-proscitech.com.au]
Sent: Thursday, September 14, 2000 9:53 PM
To: 'Ritchie Sims'; Ekstrom, Harry; Microscopy-at-sparc5.microscopy.com


Retch is right, but you could also have a poor cooling system on that pump,
or
just a badly designed system. In either case backstreaming of oil vapour
would
lead to poorly adhearing films.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Friday, September 15, 2000 6:55 PM, Retch Sims
[SMTP:r.sims-at-auckland.ac.nz]
wrote:
}
}
}
} }
} } We currently have a Bench Top Turbo carbon evaporation unit made by
Denton.
} } We are very displeased with it. We consistently get non uniform
coatings
} } that peel off of our metallographic mounts. Any suggestions on a
} } evaporation unit that works well or any information on 'sputtering"
carbon?
} } Any other experiences out there?
} }
}
} I would look very carefully at the cleanliness of the samples before
} coating.
} I use methanol then Freon then a dry Kimwipe, and the carbon
} coating is tenacious on brass.
}
} cheers
}
} rtch
}
}
} Ritchie Sims Phone : 64 9 3737599 ext 7713
} Department of Geology Fax : 64 9 3737435
} The University of Auckland email : r.sims-at-auckland.ac.nz
} Private Bag 92019
} Auckland
} New Zealand




From daemon Fri Sep 15 08:11:00 2000



From: donald j marshall :      dmrelion-at-world.std.com
Date: Fri, 15 Sep 2000 08:56:12 -0400 (EDT)
Subject: full address

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

Much as I dislike cluttering up the list with complaints, I would really
like to see everyone following Nestor's recommended guidelines which include
posting the sender's full address, including geographical. Sometimes,
obviously, we can tell from the email address but all too often we can't;
and sometimes we cannot even tell the country of origin, much less the city
and state. This information is especially when posting persons are seeking
help with instrument problems.

I realize that full address is an option on many email programs, and I
sometimes forget to choose it myself, but I urge everyone to give it more
thought.

Thank you.

Don Marshall

Donald J. Marshall
Relion Industries
P.O. Box 12
Bedford, MA 01730
Ph: 781-275-4695
FAX: 781-271-0252
email dmrelion-at-world.std.com

Cathodoluminescence, mass spectroscopy, electron beam technology


"A weed is a flower out of place."

(Please note: Do not send email with attachments to this address. Instead, send it to dmrelion-at-aol.com. Thank you.)



From daemon Fri Sep 15 08:13:49 2000



From: Anthony Garratt-Reed :      tonygr-at-mit.edu
Date: Fri, 15 Sep 2000 09:02:26 -0400
Subject: Re: LN2 and Contamination

Contents Retrieved from Microscopy Listserver Archives
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The turbo pump is simply better than the diffusion pump at minimizing back
streaming. Will Bigelow in his book on Vacuum Methods in Electron
Microscopy talks about this and describes the care you should take in
designing a pump-down protocol to minimize the backstreaming.

Tony.

}
} Hi, Ron and all
}
} Your first sentence is interesting, if I may stray a bit from the
} actual topic.
} I had formed the idea that the back-streamed oil is not diff pump
} fluid, but rotary pump oil.
} But if the phenomenon disappears when a turbo or ion pump replaces
} the diff pump, then that kindof exonerates the rotary pump, doesn't
} it?
} Or is it just that the turbo or ion pump is better than the diff pump
} at preventing rotary pump vapour from diffusing backwards through it?
} Anybody know the definitive answer to this?
}
}


** Anthony J. Garratt-Reed
** MIT Room # 13-1027
** 77 Massachusetts Avenue
** Cambridge, MA 02139-4307
** USA
**
** Phone: (+) 1-617-253-4622
** Fax: (+) 1-617-258-6479
**





From daemon Fri Sep 15 08:15:27 2000



From: tellis2-at-hallmark.com
Date: Fri, 15 Sep 2000 08:05:16 -0500
Subject: dye sub printer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I use a Sony Mavigraph digital printer model UP-D1500 to print images
from our SEM, its been reliable for the 4 years we have had it. I don't
publish (industrial use- trade secrets, industrial hygiene, quality type
stuff) so the resolution limit is OK for our use. The only problem that I
have had is the print paper tends to curl sometimes and won't feed unless
you straighten them.
I also use a Sony Mavigraph color video printer model UP-3000 to
print images from our light microscopes through a video camera and
analog-digital converter and its been reliable and no problems at all. They
both cost about $3000 when I bought them.
Terry Ellis
Of course I have no interests in that company, wish I did.




From daemon Fri Sep 15 09:06:17 2000



From: Lauran Oomen :      Oomen-at-nki.nl
Date: Fri, 15 Sep 2000 15:56:33 +0200
Subject: LM - Need Optical Density Standard

Contents Retrieved from Microscopy Listserver Archives
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Dear all,

Can someone provide me with information on how to obtain microscope
slides which can serve as standards for measuring optical density in
transmission light microscopy? We would like to use these as a staining
independent standard for the imaging and image analysis of
histochemically stained control and experimental slides. Are there any
commercially available ones? Alternative: can someone give me "recipes"
for home made ones? We were thinking of eventually using small pieces of
light exposed and developed film or neutral gray filters. Has anyone
tried this already?

Thanks in advance.

****************************************************
Lauran C.J.M. Oomen oomen-at-nki.nl
The Netherlands Cancer Institute tel. +31 20 5121889
Digital Microscopy Facility fax. +31 20 5121893
Plesmanlaan 121
1066CX Amsterdam
The Netherlands
****************************************************





From daemon Fri Sep 15 09:22:47 2000



From: NPGSlithography-at-aol.com
Date: Fri, 15 Sep 2000 10:14:30 EDT
Subject: Re: Anybody else want a PCD?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} Further to my posting yesterday seeking a PCD for a JEOL 840, which,
} I am informed, is the same as that for a 6400, I am currently
} negotiating with a company to make me one.
}
} Does anyone else out there want one?

While I am not looking for a PCD myself, I have customers who may be
interested. If you can let me know the company you are dealing with I would
appreciate it.

Joe
_________________________________________
Joe Nabity, Ph.D.
JC Nabity Lithography Systems
E-Beam Lithography using Commercial SEMs & STEMs
PO Box 5354, Bozeman, MT 59717 USA
Voice: (406) 587-0848
FAX: (406) 586-9514
E-mail: info-at-jcnabity.com
Web: www.jcnabity.com



From daemon Fri Sep 15 10:09:58 2000



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Fri, 15 Sep 2000 10:01:19 -0500
Subject: Introducing water vapor to a VP-SEM

Contents Retrieved from Microscopy Listserver Archives
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I have just had a user approach me about looking at a water-saturated
sample in our Hitachi S-2460N. While we have used VP-SEM for several years
to look at insulating samples, this is the first time we have been asked to
look at a moist sample. I have two questions as I plunge into this.

1-What instrumental concerns are there related to using water vapor as an
atmosphere? I have heard that it may sorb onto the chamber surfaces and may
be slow to desorb, so our ultimate vacuum may be poor for a while
afterwards. There was also a concern over possible effects of the vapor on
the precision leak valve. Are there any other components that might be
affected, e.g., EDS window, BSE detector, etc.? Since the water will be
present as vapor at less than 2 torr and will only be used for about an
hour I don't envision problems, but I would rather not be surprised.

2-What are effective ways for introducing water vapor into the SEM? I am
considering partially filling a vacuum desiccator with water and hooking
our vent line to it and allowing the SEM vacuum to vaporize the water.
Someone suggested a moistened rag in the vent line. What other means work
well?

Thank you in advance.
----------------------
Warren E. Straszheim
Materials Analysis and Research Lab
Iowa State University
23 Town Engineering
Ames IA, 50011-3232

Ph: 515-294-8187
FAX: 515-294-4563

E-Mail: wesaia-at-iastate.edu
Web: www.marl.iastate.edu

Scanning electron microscopy, x-ray analysis, and image analysis of materials
Computer applications and networking



From daemon Fri Sep 15 10:32:19 2000



From: attz-at-pacbell.net
Date: Fri, 15 Sep 2000 08:19:12 -0700
Subject: FIB: Need help with Cu grain imaging

Contents Retrieved from Microscopy Listserver Archives
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I am a graduate student in the Materials Engineering Department at San
Jose State University in California, and need some help with my thesis
research. I was wondering if anyone out there has any experience with
FIB imaging of recrystallized Cu films for the purposes of grain sizing.
My research requires both plan view (at various tilt angles) and
cross-sectional views of blanket films. I plan to work with an outside
lab that is very experienced with SEM imaging, but relatively
inexperienced with FIB imaging. They just got their first system online
a couple of months ago. My questions are related to sample preparation
from an 8" Si wafers and suggested FIB operating parameters, tips, and
tricks to get quality images with good grain-to-grain contrast. If
anyone has such experience or can point me in the right direction, it
would be greatly appreciated. I am also seeking a good reference book
or review article that covers FIB imaging theory (especially Ga ion
channeling contrast). You can respond to the listserver, or contact me
directly at attz-at-pacbell.net. Thanks in advance for your time and
help.

Andrew Tzanavaras
San Jose State University






From daemon Fri Sep 15 11:01:44 2000



From: Helvey, Marc :      Marc.Helvey-at-vlsistd.com
Date: Fri, 15 Sep 2000 09:00:20 -0700
Subject: Fourier Transform Infrared Spectroscopy (FTIR)

Contents Retrieved from Microscopy Listserver Archives
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LEE FILTERS
Central Way
Walworth industrial Estate
Andover
Hampshire
SP10 5AN

http://www.leefilters.co.uk/

Lee make optical density filters in polyester squares 75mmx75mm
which can be cut up into small squares or circles (paper punch)
and mounted under a coverslip on a microsope slide. If you want to
avoid contributions to the density from glass, mountant, etc drill an
array of holes in a microscope slide sized piece of aluminium
sheet, and mount samples of the filters over the apertures

Kodak also make ND Wratten filters in gelatin
Chris


Date sent: Fri, 15 Sep 2000 15:56:33 +0200
} From: Lauran Oomen {Oomen-at-nki.nl}
To: Microscopy-at-sparc5.microscopy.com



Good theory page:
http://sis.bris.ac.uk/~sd9319/spec/IR.htm


Instrument Manufacturers:

Block Engineering
http://www.blockeng.com/index2.htm

Nicolet
http://www.nicolet.com/

Bio-Rad
http://www.bio-rad.com

Bruker Optics
http://www.bruker.com/optics/

Bomen
http://www.bomem.com/

Good Luck -

Marc Helvey
Sales Accounts Manager
VLSI Standards, Inc.
3087 North First Street
San Jose, CA 95134-2006
Phone: (408) 428-1800, ext. 108
FAX: (408) 428-9555
Mobile: (408) 307-3833
E-Mail: marc.helvey-at-vlsistd.com {mailto:marc.helvey-at-vlsistd.com}
Internet: http://www.vlsistd.com
 
 


-----Original Message-----
} From: Martin Izquierdo [mailto:MartinI-at-oztek.com]
Sent: Thursday, September 14, 2000 6:14 PM
To: 'Microscopy-at-MSA.Microscopy.Com'


Does anyone have any information on Fourier Transform Infrared (FTIR)
Spectroscopy used for organic contamination analysis? Specifically
manufacturers and the appx. price for the equipment .
Any information would be helpful.

Martin A. Izquierdo
Analytical Laboratory
Cerprobe, Inc.




From daemon Fri Sep 15 11:12:50 2000



From: Pauline C. Yu :      splene-at-pw.usda.gov
Date: Fri, 15 Sep 2000 09:06:16 -0700 (PDT)
Subject: Re: Dye Sublimation Printer vs. Epson

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Richie and Listers

The answer is that rotary pump oil is able to pass through diffusion pumps
easier than turbo pumps. Michael Postek of NIST wrote on article on this.
"An Approach to the Reduction of Hydrocarbon Comtamination in the Scanning
Electron Microscope" Scanning Vol. 18, 269-274 (1996). One of the techniques
he used to reduce oil comtamination was to create a leak in the foreline of
the Diff. Pump to create viscous flow conditions that stopped the
backstreaming of rotary pump oil into the Diff. pump. This stopped
condensation of pump oil on his EDS detector window.

I have a box of reprints of this article in my office, since it also
discussed nitrogen purging which was used in my original SEM-CLEAN product.
So if any one wants one, e-mail me back and ask for the "Postek" article.
Please include your mailing address.

My web site SEMCLEAN.COM has full information on XEI's anti-contamination
systems. No LN or turbopump required.

Ronald Vane
XEI Scientific
3124 Wessex Way
Redwood City, CA 94061
(650)369-0133

-----Original Message-----
} From: Ritchie Sims {r.sims-at-auckland.ac.nz}
To: message to: MSA list {microscopy-at-sparc5.microscopy.com}


I'm putting in my 2 cents on this thread because we have both in our lab.
This is Tektronics Phaser 450 vs. the Epson 1270. The 1270 has cheaper
supplies, more variety of papers, larger format and much much higher
stability in it's favor. And the 450? I have to say I still prefer the
dye-subs grey tones for images over the "grittiness" of the inkjet. The
dye sub print still looks more like an RC print to me.
When I requested materials from Epson demonstrating the capabilities of
the 1270 and 870, we got this amazing test print from them where the
gradation of tones really was spectacular. None of the prints from our
own photos look nearly that good on the Epson, and in side by side
comparisons with the dye sub, I think the dye sub prints always look
better.

I guess it boils down to price considerations, is that extra $1.50 per
print + the difference in cost between printers worth it?

Pauline Yu
Microscopist Technician
USDA-ARS-WRRC
pyu-at-pw.usda.gov




From daemon Fri Sep 15 11:47:10 2000



From: Debby Sherman :      sherman-at-btny.purdue.edu
Date: 15 Sep 2000 11:39:31 -0500
Subject: Re: serial section pick-up

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------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America
Mark,
I sent the method below to the list some time ago. It works great.
Debby Sherman

A Fool-proof Method for Mounting Serial Sections on Single Hole Grids

I did serial sectioning for years on large single hole grids using a very simple technique that made the potential problems of film thickness, wrinkles and section loss very minor. I was not the original developer of the method and do not remember who originally gave it to me. It goes as follows:

1) Have your machine shop cut some thin pieces of Plexiglas into the size of glass slides. At one end, drill about a dozen holes, roughly 5mm in diameter, in an area about the size of a formvar film cast on glass slides. These slides will serve as your template for holding your films.

2) Cast the formvar films onto glass slides using your normal method. Usually a good silver film, not gray, will work fine. I routinely used 0.2% formvar in dichloroethane when casting by immersing the slide into the solution in a small jar, etc. We now use a film caster that lets us hold the slide in the dichloroethane vapors after lowering the formvar solution level. This method tends to give you thinner films consistently so the correct solution percentage and timing would have to be redetermined.

3) Float the film off the glass slide and pick it up with the Plexiglas slide so the film covers the holes. Then draw the water out of the holes by pressing the plastic slide down onto filter paper, or using small pieces of filter paper and capillary action to draw the water out of individual holes. The films should hold nicely over the holes in the slide. Store slides until needed.

4) Next, cut your sections using a block diameter that is fairly similar to the size of the slit in the grid. Pick up the sections on UNCOATED grids by gently lowering the grid to the surface of the knife boat. I put the dull side down on the premise that the rough surface would grab the film better during step 6. The surface tension of the water will hold the sections in the grid opening. Transfer the grid to a droplet of water until you have finished sectioning. Do not invert the grid. It is important that the top of the grid (shiny side) stay dry so that the grid will float on all subsequent solutions.

5) Transfer the grid + sections + water droplet to a drop of stain. A small amount of water will be transferred but this will not interfere with staining. If you are concerned about the dilution effect, increase your staining time slightly. Allow the section to stain, then wash by transferring through a series of droplets of clean water. Continue to post-stain if desired and wash the same way. Never let the grid dry. There is minimum problem with stain precipitation if you use very clean water and transfer the grid through a sufficient number of water droplets (6-12 recommended).

6) The final step is to transfer the grid to a film suspended over the hole in a Plexiglas slide and let it dry down. The sections will now be stuck to the film with NO wrinkles and minimum breakage. When ready to view, just punch out around the grid with the tip of your forceps, grab the grid and insert into the microscope.

Believe me....the sections will still be there at the end!

I found that as long as the sections cover a substantial portion of the open area of the grid, carbon coating was not essential. I used to do 50-100 grids worth of serial sections without loosing any. The films on the plastic slides would hold for months so I could make a lot and store until needed. The method really works...do give it a try.


Debby Sherman
Microscopy Center in Agriculture
Dept. of Botany & Plant Pathology
Purdue University
West Lafayette, IN 47907


Published:
Sherman, D.M. (1998) A Full-proof Method for Mounting Serial Sections on Single Hole Grids. MSA Technologist’s Forum Newsletter 16:2




} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hi,
}
} Well, thanks for the flood of suggestions about getting a ribbon. I
} trimmed the block sides with a glass knife (and enjoyed Mike Delannoy's
} suggestion of 'trimming the top and bottom sides of your face with a glass
} knife' - didn't know microscopy was such a painful job) and quite a decent
} ribbon was the result. I tried the chloroform-glue idea and that seemed to
} make the sections stick together more, but sometimes they gummed up a bit
} on the knife. The next battle is picking up the ribbon on several slot
} grids - I suspect that it's more practice than anything else. The method I
} was shown was to go underneath the ribbon at an angle with the grid, raise
} it until the waterline is on the top of the exposed formvar of the slot,
} push the ribbon onto the waterline and draw the grid up at the same time,
} and touch the ribbon with the hair at the point where the slot finishes to
} break the ribbon, and remove the slot - all easier said than done! Any
} ideas would be much appreciated.
}
} Thanks,
}
} Mark
}
} ********************************************
} Mark West,
} Unidad de Microscopia Electronica,
} (Electron Microscopy Unit)
} Instituto de Fisiologia Celular,
} Universidad Nacional Autonoma de Mexico,
} 04510 Mexico D.F.
}
} tel (unidad/lab) *(525) 622 5610*
} (casa/home) (525) 619 3020
} Fax (525) 616 2282
} ********************************************






From daemon Fri Sep 15 12:25:54 2000



From: Shalvoy, Richard B **CHES :      RBShalvoy-at-archchemicals.com
Date: Fri, 15 Sep 2000 12:17:21 -0500
Subject: Dye Sub Printer Speeds

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Message-ID: {7531557A3D91D311B0320008C7E6708611A415-at-arch-exc-05.archchemicals.com}


I have a Kodak 8670 dye sub printer hooked up to my SEM, but it seems to me
that it takes a long time to get it to print and also while it is in the
printing process my computer (and hence my SEM) is locked up. Here are the
details.

I have the printer (1 year old) hooked to a PC with a 333 Pentium II
processor (128 MB RAM) using a parallel cable. I do get the same
performance when printing from other computers so I doubt that it is
anything peculiar to this one computer.

Now when I call for a printout it takes 5 minutes to get the 1MB file size
print done, and almost all of the time is spent sending data to the printer
- the actual printing takes a short time (30 sec). I have discussed this
with Kodak Tech Support and they claim my performance is not unusual.
HOWEVER, I also experienced much quicker printing times (I'm sure - I have
notes, but it was a while ago) when I was first using the printer (1 minute
/ 1 MB of file size in the image) and this is confusing me.

My question is: how fast should this sort of printer print when using the
parallel cable to feed data to it? Is 5 minutes / 1MB unusually slow? I
get frustrated particularly as my computer is locked up while the data is
being sent out and I cannot continue imaging until the data dump is done. I
have the Win 95 spooler running of course and I don't have this problem with
my HP 970 inkjet which I use normally.

I will welcome any good ideas or cold realities that apply.

Richard Shalvoy
Arch Chemicals
350 Knotter Drive
Cheshire, CT 06410
203-271-4394



From daemon Fri Sep 15 13:19:42 2000



From: Wil Bigelow :      bigelow-at-engin.umich.edu
Date: Fri, 15 Sep 2000 13:14:29 -0400
Subject: RE: Oil backstreaming & TMPs

Contents Retrieved from Microscopy Listserver Archives
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The reason that you can back up a turbomolecular pump with an oil-sealed
rotary vane pump and still have a system that is virtually free of oil
contamination is that the compression ratio of turbo pumps varies
exponentially with the square root of the molecular mass of the molecules
being pumped. Thus, for nitrogen, which has M = 28, this value is about
200, whereas for a hydrocarbon molecule such as octane with M = 66 it is
greater than 3000, and for a molecule of pump oil with M greater than 100
it is greater than a million. These values are for a single compression
stage (rotor disk) of a turbo pump. Actual turbo pumps have from 15 to 20
such stages which act in series, so the overall value for nitrogen ends up
being around 10^9, while for hydrocarbons it becomes astronimical (10^40 is
often cited). Thus, if a turbo pump that is backed by a rotary vane pump is
managed so that the rotor never drops below about 75% of full operating
speed while the pressure in it is in the molecular flow range (below about
1 Torr is a safe figure), it is virtually impossible for oil molecules to
backstream through it. These matters are all discussed in detail on pages
229 to 234 of my book 'Vacuum Methods in Electron Microscopy' (see
http:/www.2spi.com/catalog/books/book48.html, and
http:/pup.princeton.edu/titles/6484.html for a description) and
appropriate operating protocols are are described on pp. 249 to 253 .

Wilbur C. Bigelow, Prof. Emeritus
Materials Sci. & Engr., University of Michigan
Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu;
Fx:734-763-4788; Ph:734-662-5237





From daemon Fri Sep 15 13:37:01 2000



From: David Henriks :      Henriks-at-CompuServe.COM
Date: Fri, 15 Sep 2000 14:28:09 -0400
Subject: Carbon Evaporation Units/Sputtering

Contents Retrieved from Microscopy Listserver Archives
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Dear Chao-Ying:

Actually, any target material, including carbon and other high purity
materials, that can be used in a vacuum evaporator or DC diode coater can
be utilized in an Ion Beam Sputter Deposition System.

As for carbon, TEM electron diffraction images of ion beam sputtered carbon
films are amorphous. The structure of a carbon grid before the carbon film
was deposited cannot be distinguished from the carbon film!

TYPICAL SPUTTER RATES

Target Å/min

Cr 8-12
W 8-10
Ta 8-10
Pt 10-12
Ir 10-12
C 2-6

DISCLAIMER: South Bay Technology produces the IBS/e Ion Beam Sputter
Deposition and Etching System for high resolution microscopy applications
as described above and, therefore, has a vested interest in promoting its
use.

Best regards-

David
Writing at 11:12:20 AM on 09/15/2000

***************************************************************************
************************

David Henriks
Vice President TEL: 800-728-2233
(toll free in the USA)
South Bay Technology, Inc. +1-949-492-2600
1120 Via Callejon FAX: +1-949-492-1499
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

***************************************************************************
************************

} } } } } Please visit us at http://www.southbaytech.com { { { { {

Manufacturers of precision sample preparation equipment and supplies for
metallography, crystallography and electron microscopy.


Message text written by "Ni, Chao-Ying"
}
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Harry,

To the best of my knowledge, there is no such thing as "sputtering carbon".
But I would suggest to look into your carbon source. Try different vendors.
Also, make sure your vacuum is good.

Have a nice day!

Chao-Ying Ni
Rodel Inc.
451 Bellevue Road
Newark, DE 19713
(302) 366-0500 ext 2812
{




From daemon Fri Sep 15 14:29:00 2000



From: Lucille A. Giannuzzi :      lag-at-mail.ucf.edu
Date: Fri, 15 Sep 2000 15:18:30 -0400
Subject: salaries for staff

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Facility Directors,

We currently use staff engineers to service instruments, assist in teaching
classes, train users on operating equipment, and performing some minor
administrative tasks (primarily in the physical sciences area). We have 12
pieces of analytical equipment and only 4 instruments on service contracts,
so we rely heavily on our staff to keep our instruments running. I have
performed some surveys from industry and service engineer pay scales vary
approximately in the following manner:

$30's-40's: for starting engineers with little to no experience that
require training
mid $40's to mid $50's: for an experienced engineer
mid $50's to mid $70's: for a very skilled engineer who can trouble shoot
to the component level independently without any back-up help.

It would be of great help to me and my staff if you could take a few
minutes and enlighten me as to your approximate salary ranges for your
staff compared to their experience level, and compared to the numbers I
have shown above.

Thanks very much for your time and help.

Regards,
Lucille Giannuzzi

*******************************************************************
Lucille A. Giannuzzi, Ph.D.

Associate Professor, Mechanical, Materials, and Aerospace Engineering
Director, UCF/Cirent Materials Characterization Facility
University of Central Florida
12443 Research Parkway, Suite 305
Orlando, FL 32826 email lag-at-mail.ucf.edu phone (407) 275-4354,5,6
fax (407) 275-4321
--------------------------------------------------------------------
"Good judgement comes from experience.

Experience comes from making bad judgement."

Mark Twain
********************************************************************





From daemon Fri Sep 15 16:28:05 2000



From: John J. Bozzola :      bozzola-at-siu.edu
Date: Fri, 15 Sep 2000 16:15:36 -0500
Subject: Epson Archival Inks

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


A colleague wishes to print "archival" quality grey scale images
(micrographs) on an Epson 750 Photo printer. They have purchased CMY
archival quality inks from a company called Lysonic. They are
apparently unable to obtain a gray scale image but instead obtain one
that is "off color". According to my colleague, the printer will not
generate an image using the Black ink cartridge but only when CMY
inks are used. Has anyone solved this problem (of color balance)?

Thank you,


####################################################################
John J. Bozzola, Ph.D., Director
Micro-Imaging and Analysis Center
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
####################################################################



From daemon Fri Sep 15 16:29:17 2000



From: Chris Jeffree :      cjeffree-at-srv0.bio.ed.ac.uk
Date: Sat, 16 Sep 2000 00:16:24 +0100
Subject: Re: LM - Need Optical Density Standard

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


http://www.kodak.com/cgi-bin/searchKodak.cgi?searchText=neutral+density+fi
lters+
will get you on a good start. I tried to send it direct and couldn't
resolve your address.

Good luck
Gordon
Gordon Couger gcouger-at-couger.com
Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00

----- Original Message -----
} From: "Lauran Oomen" {Oomen-at-nki.nl}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Friday, September 15, 2000 8:56 AM


Edmund Scientific sell stepped neutral density filters with 11 discrete
density steps in intervals of 0.1 OD which might be useful here,
though the OD range may not be wide enough for all applications.

These filters are coated on glass 2" x 1" x 0.062" thick, cover the OD
range 0.04 (92%) to 1.0 (10% transmittance). They are spectrally
flat in the range 400 to 700nm. Tolerances are not stated, but they
are recommended as "Ideal for applications requiring variable
densities and known density values". £119 in UK, which probably
translates into $119 in US.


} } Dear all,
} }
} } Can someone provide me with information on how to obtain microscope
} } slides which can serve as standards for measuring optical density in
} } transmission light microscopy? We would like to use these as a staining
} } independent standard for the imaging and image analysis of
} } histochemically stained control and experimental slides. Are there any
} } commercially available ones? Alternative: can someone give me "recipes"
} } for home made ones? We were thinking of eventually using small pieces of
} } light exposed and developed film or neutral gray filters. Has anyone
} } tried this already?
} }
} } Thanks in advance.
} }
} } ****************************************************
} } Lauran C.J.M. Oomen oomen-at-nki.nl
} } The Netherlands Cancer Institute tel. +31 20 5121889
} } Digital Microscopy Facility fax. +31 20 5121893
} } Plesmanlaan 121
} } 1066CX Amsterdam
} } The Netherlands
} } ****************************************************
} }
} }
} }
}
}
}
}
}


~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Dr Chris Jeffree
University of Edinburgh
Biological Sciences EM Facility
Daniel Rutherford Building
King's Buildings EDINBURGH EH9 3JH
Tel: +44 (0) 131 650 5345
FAX: +44 (0) 131 650 6563

Inveresk Cottage, 26 Carberry Road,
Inveresk, Musselburgh, Midlothian EH21 8PR, UK
Tel: +44 (0) 131 665 6062 / Mobile 07710 585 401
FAX: +44 (0) 131 653 6248
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~



From daemon Fri Sep 15 21:25:30 2000



From: COURYHOUSE-at-aol.com
Date: Fri, 15 Sep 2000 22:08:00 EDT
Subject: Re: Epson Archival Inks

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


When in doubt always buy a hp printer.
I would look to see what solution hp is providing these days.

Ed Sharpe archivist for SMECC



From daemon Fri Sep 15 23:15:59 2000



From: John Mansfield :      jfmjfm-at-umich.edu
Date: Sat, 16 Sep 2000 00:01:27 -0400
Subject: Epson Printers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Dr. John Mansfield CPhys MInstP
North Campus Electron Microbeam Analysis Laboratory
417 SRB, University of Michigan
2455 Hayward, Ann Arbor MI 48109-2143
Phone: (734) 936-3352 FAX (734) 763-2282
Cellular Phone: (734) 358-7555
(Leaving a phone message at 936-3352 is preferable to 358-7555)
Email: jfmjfm-at-engin.umich.edu
URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html
Location: Lat. 42° 16' 48" Long. 83° 43' 48"



From daemon Fri Sep 15 23:18:50 2000



From: Sally Stowe :      STOWE-at-rsbs.anu.edu.au
Date: Sat, 16 Sep 2000 15:06:16 +1100
Subject: Re: Introducing water vapor to a VP-SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Warren -

We use a Hitachi 2250-N with a variety of gases. Attaching the inlet line
to a desiccator or heavy conical flask of water works fine, but the vacuum
gauge calibration may need checking if you need to know pressure
accurately.

If you usually run with air or nitrogen in VP conditions, you will
probably find the water gives you a resolution advantage (Helium is even
better).

However your user should be aware that one or two torr of water does not
do a lot to keep a specimen moist unless you also use a cold stage.

good luck,

Sally

Sally Stowe
EM Unit
Australian National University
Canberra








} } } Warren E Straszheim {wesaia-at-iastate.edu} 09/16/00 02:01am } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


I have just had a user approach me about looking at a water-saturated
sample in our Hitachi S-2460N. While we have used VP-SEM for several years

to look at insulating samples, this is the first time we have been asked to

look at a moist sample. I have two questions as I plunge into this.

1-What instrumental concerns are there related to using water vapor as an
atmosphere? I have heard that it may sorb onto the chamber surfaces and may

be slow to desorb, so our ultimate vacuum may be poor for a while
afterwards. There was also a concern over possible effects of the vapor on

the precision leak valve. Are there any other components that might be
affected, e.g., EDS window, BSE detector, etc.? Since the water will be
present as vapor at less than 2 torr and will only be used for about an
hour I don't envision problems, but I would rather not be surprised.

2-What are effective ways for introducing water vapor into the SEM? I am
considering partially filling a vacuum desiccator with water and hooking
our vent line to it and allowing the SEM vacuum to vaporize the water.
Someone suggested a moistened rag in the vent line. What other means work
well?

Thank you in advance.
----------------------
Warren E. Straszheim
Materials Analysis and Research Lab
Iowa State University
23 Town Engineering
Ames IA, 50011-3232

Ph: 515-294-8187
FAX: 515-294-4563

E-Mail: wesaia-at-iastate.edu
Web: www.marl.iastate.edu

Scanning electron microscopy, x-ray analysis, and image analysis of
materials
Computer applications and networking




From daemon Fri Sep 15 23:25:04 2000



From: donald j marshall :      dmrelion-at-world.std.com
Date: Sat, 16 Sep 2000 05:03:46 -0400 (EDT)
Subject: looking for something POS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Martin -

FTIR is a very useful tool for analysis of organic contaminants. All FTIR
systems consist of a spectrometer, or bench. The bench can be fitted with
various accessories -- e.g., a beam condenser, in-compartment ATR,
microscope, etc. -- that permit analysis of different states and amounts of
materials. Analysis of contamination likely will require use of one or more
of these sampling accessories. Manufacturers such as Nicolet, SensIR,
Perkin-Elmer, Biorad, and other third-party vendors can provide
specifications and application notes for each. The total system cost will
depend largely on the sampling accessory and other add-ons such as cameras
and databases. A standard bench and beam condenser or in-compartment ATR
might cost $25K to $30K. Some benches and in-compartment accessories are
sufficiently portable for field use (SensIR makes a portable diamond ATR
system with integrated video that is quite nice). If your samples are
routinely less than 100 microns in diameter or consist of multiple phases or
layers, a microscope accessory likely will be required. A standard bench
and microscope might cost $65K to $85K or more; add multiple detectors,
objectives, and automated operation for QC work and the cost can exceed
$125K.

I use a portable Nicolet bench coupled to a high-end Nicolet/Spectra-Tech
microscope. The bench can be taken on-site for analysis of larger
homogenous samples. The microscope is used in the lab for analysis of
microscopic samples, including individual fibers or layer in composites.
Dual detectors in the microscope cover a spectral range of 4000-450
wavenumbers for organic and many inorganic analyses, and imaging is enhanced
by infinity-corrected optics, fluorescence and visible polarized
illumination, and a digital/video imaging system.

Whatever you decide best suits your needs, I suggest you visit a
manufacturer to test drive their equipment using your own samples. Most are
only too pleased to assist.

Hope this helps, and good luck.

James Martin
Principal/Research Scientist
Orion Analytical, LLC
PO Box 550
Williamstown, MA 01267
www.orionanalytical.com

----- Original Message -----
} From: Martin Izquierdo {MartinI-at-oztek.com}
To: 'Microscopy-at-MSA.Microscopy.Com' {Microscopy-at-sparc5.microscopy.com}
Sent: Thursday, September 14, 2000 9:13 PM


Hi,

I am interested in obtaining one or more Olympus POS (vertical tue monocular
with rotating stage) microscopes in good condition. Anyone have one that is
no longer being used?

Thanks.

Don Marshall


Donald J. Marshall
Relion Industries
P.O. Box 12
Bedford, MA 01730
Ph: 781-275-4695
FAX: 781-271-0252
email dmrelion-at-world.std.com

Cathodoluminescence, mass spectroscopy, electron beam technology


"A weed is a flower out of place."

(Please note: Do not send email with attachments to this address. Instead, send it to dmrelion-at-aol.com. Thank you.)



From daemon Sat Sep 16 13:18:03 2000



From: Harrison :      tuttle-at-home.com
Date: Sat, 16 Sep 2000 13:01:58 -0500
Subject: Re: Epson Archival Inks

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Speaking of such, check out this story at
http://www.cnn.com/2000/TECH/computing/09/14/epson.image.fading.idg/ind
ex.html

Ozone fades some Epson photos

Contrary to ads promising fade-resistant, long-lasting photos, color prints
made with several Epson Stylus Photo ink jets turn orange in locations with
heavy concentrations of ozone in the air, Epson officials confirm. The
company offers to buy your printer if you're not satisfied with several
recommended fixes, including using new types of paper Epson will introduce
this fall. The problem came to light when some owners of Epson's Stylus
Photo 870, 875DC, and 1270 reported photos they printed were turning
orange.

Dave Harrison





From daemon Sat Sep 16 19:34:18 2000



From: Larry Allard :      l2a-at-ornl.gov
Date: Sat, 16 Sep 2000 20:10:21 -0400
Subject: Re: Epson Printers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} } John writes: } } When in doubt NEVER buy a hp printer.
The HP1600 what a piece of the proverbial garbage! { {

John...you've gotta stop sugar-coating everything...just tell it like it is!

Larry ;-)


}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Dr. Lawrence F. Allard
Senior Research Staff Member
High Temperature Materials Laboratory
Oak Ridge National Laboratory
1 Bethel Valley Road
Bldg. 4515, MS 6064
PO Box 2008
Oak Ridge, TN 37831-6064

865-574-4981
865-576-5413 Fax
allardlfjr-at-ornl.gov



From daemon Sun Sep 17 17:28:22 2000



From: Andrew McNaughton :      andrew.mcnaughton-at-stonebow.otago.ac.nz
Date: Mon, 18 Sep 2000 10:07:44 +1200
Subject: Online booking sheets.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello

As part of a gradulal upgrading of facilites, we're considering
putting our booking sheets online to allow users to book themselves
on the TEM, SEM, confocal or LM from the comfort of their own office.

Can anyone recommend Macintosh software capable of doing this?
Ideally something which can be accessed by anyone with a web browser
to avoid having to install new software on everyones computer.

Any feedback as to whether this sort of system really is better than
a piece of paper nailed to the wall with a pen on a string would be
welcomed too.

Thank you.

Andrew McNaughton
--
______________________________________________________________________________
Andrew McNaughton
South Campus Electron Microscope Unit
C/-Department of Anatomy and Structural Biology
School of Medical Sciences
University of Otago
PO Box 913, Dunedin
NEW ZEALAND

Telephone: +64 3 479 7308
Facsimile: +64 3 479 7254

e-mail: andrew.mcnaughton-at-stonebow.otago.ac.nz
______________________________________________________________________________




From daemon Sun Sep 17 19:11:28 2000



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Mon, 18 Sep 2000 11:59:37 GMT+1200
Subject: RE: Oil backstreaming & TMPs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thank you Wil

Is there a corresponding picture for a diff pump ie why do they let
RP oil through?
That's assuming, of course, that the oil that builds up on eds
windows IS RP oil. It is, isn't it?

cheers

rtch


}
} The reason that you can back up a turbomolecular pump with an oil-sealed
} rotary vane pump and still have a system that is virtually free of oil
} contamination is that the compression ratio of turbo pumps varies
} exponentially with the square root of the molecular mass of the molecules
} being pumped. Thus, for nitrogen, which has M = 28, this value is about
} 200, whereas for a hydrocarbon molecule such as octane with M = 66 it is
} greater than 3000, and for a molecule of pump oil with M greater than 100
} it is greater than a million. These values are for a single compression
} stage (rotor disk) of a turbo pump. Actual turbo pumps have from 15 to 20
} such stages which act in series, so the overall value for nitrogen ends up
} being around 10^9, while for hydrocarbons it becomes astronimical (10^40 is
} often cited). Thus, if a turbo pump that is backed by a rotary vane pump is
} managed so that the rotor never drops below about 75% of full operating
} speed while the pressure in it is in the molecular flow range (below about
} 1 Torr is a safe figure), it is virtually impossible for oil molecules to
} backstream through it. These matters are all discussed in detail on pages
} 229 to 234 of my book 'Vacuum Methods in Electron Microscopy' (see
} http:/www.2spi.com/catalog/books/book48.html, and
} http:/pup.princeton.edu/titles/6484.html for a description) and
} appropriate operating protocols are are described on pp. 249 to 253 .
}
} Wilbur C. Bigelow, Prof. Emeritus
} Materials Sci. & Engr., University of Michigan
} Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu;
} Fx:734-763-4788; Ph:734-662-5237
}
}
}
}
Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand



From daemon Mon Sep 18 01:18:31 2000



From: Gordon Couger :      gcouger-at-couger.com
Date: Mon, 18 Sep 2000 00:55:01 -0500
Subject: Re: Online booking sheets.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} From: "Andrew McNaughton" {andrew.mcnaughton-at-stonebow.otago.ac.nz}

} Hello
}
} As part of a gradual upgrading of facilities, we're considering
} putting our booking sheets online to allow users to book themselves
} on the TEM, SEM, confocal or LM from the comfort of their own office.
}
} Can anyone recommend Macintosh software capable of doing this?
} Ideally something which can be accessed by anyone with a web browser
} to avoid having to install new software on everyones computer.
}
} Any feedback as to whether this sort of system really is better than
} a piece of paper nailed to the wall with a pen on a string would be
} welcomed too.
}
Andrew,

A web page that emulated the sheet nailed to the wall should work fine and
the use won't have to walk to the machine to sign up. I think it is a
pretty simple job to write there should be dozens of kids around that can
do it. Just don't let them try to improve on the sheet of paper on the
wall concept or the users will get upset.

Gordon
Gordon Couger gcouger-at-couger.com
Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00







From daemon Mon Sep 18 06:05:23 2000



From: Carole Elleman :      c.j.elleman-at-reading.ac.uk
Date: Mon, 18 Sep 2000 11:44:12 +0100
Subject: TEM tissue culture melinex film

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear All has anyone any experience with Agar's melinex film for growing
cells on before fixation and embedding for the TEM? I would like to find out
why it is necessary to fix the film with warm fixative - doesn't this
introduce some artefacts due to membrane mobility at 37 degrees? I am
looking at membrane antigens!
Carole Elleman




From daemon Mon Sep 18 08:54:10 2000



From: R. Howard Berg :      rhberg-at-danforthcenter.org
Date: Mon, 18 Sep 2000 08:39:52 -0500
Subject: Re: Epson Archival Inks

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I recently came across this site, a source of archival gray-scale inks and
methods to employ them on an Epson...

http://www.inksupply.com/index.cfm?source=html/quadtone.html

} A colleague wishes to print "archival" quality grey scale images
} (micrographs) on an Epson 750 Photo printer. They have purchased CMY
} archival quality inks from a company called Lysonic. They are
} apparently unable to obtain a gray scale image but instead obtain one
} that is "off color". According to my colleague, the printer will not
} generate an image using the Black ink cartridge but only when CMY
} inks are used. Has anyone solved this problem (of color balance)?
}



R. Howard Berg, Ph.D.
Director, Integrated Microscopy Facility,
Associate Member
Donald Danforth Plant Science Center/Nidus Center
893 North Warson
St. Louis, MO 63141

phone: 314-812-8076
fax: 314-812-8127
cell phone: 314-378-2409

http://www.danforthcenter.org






From daemon Mon Sep 18 09:11:12 2000



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Mon, 18 Sep 2000 08:35:55 -0500
Subject: Re: Dye Sub Printer Speeds

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


1 MB/minute is just under 20K/second which is what we typically see with
our printers. It doesn't seem to matter what printers or computers we use.
We have most of our printers hooked up via ethernet connections, but even
those do not seem to be much faster than those using the parallel cable.
That surprises me since ethernet (10 Mbit/sec) should be able to handle
almost 1000K/second (if we had the whole cable to ourselves).

It seems that there must be something about Windows that restricts
throughput to 20K//sec. Perhaps someone out there knows how to speed things
up. Maybe there is some Windows setting to tweak.

The fact that your speed has dropped to about 4K/second indicates that
something must have changed - I don't know what. I am also surprised that
your computer is tied up while printing. You might want to check out your
printer properties. On the second tab (details) there is a button for spool
settings. I am used to the top selection being clicked which is "Spool
print jobs so program finishes printing faster", rather than the alternate
choice of "print directly to printer". Our programs are ready for action in
just a couple of seconds (which depends on the computer and the printing
task), and other programs continue to run even while the job is spooled up.
If the other box is checked, the computer will be tied up until the job is
fully transmitted.

I hope some of this help.

Warren

At 12:17 PM 9/15/2000 -0500, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From daemon Mon Sep 18 10:24:01 2000



From: Dusevich, Vladimir :      DusevichV-at-umkc.edu
Date: Mon, 18 Sep 2000 10:06:18 -0500
Subject: Antivibrition platforms

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All,
I beg your pardon for bringing up this issue again, but
when I did not need it, I did not pay attention to postings...

Now I have to include in a construction grant a price for
antivibration table for FEG-ESEM XL30 and, possibly,
for STEM CM12. In XL30 I have problems with vibrations
at magnifications 100-200k (and sometimes starting at 50k).

Who got good results with antivibrition platforms? What
type? Should only a column be isolated or a whole instrument?
What is a probable price range (including installation)?

Thanks,

Vladimir


Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
3127 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy

} -----Original Message-----
} From: Ni, Chao-Ying [mailto:CYNi-at-rodel.com]
} Sent: Friday, September 15, 2000 7:56 AM
} To: Ekstrom, Harry; Microscopy-at-sparc5.microscopy.com
} Cc: 'Ritchie Sims'; 'jim-at-proscitech.com.au'
} Subject: RE: Carbon Evaporation Units
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} ---------.
}
}
} Harry,
}
} To the best of my knowledge, there is no such thing as
} "sputtering carbon".
} But I would suggest to look into your carbon source. Try
} different vendors.
} Also, make sure your vacuum is good.
}
} Have a nice day!
}
} Chao-Ying Ni
} Rodel Inc.
} 451 Bellevue Road
} Newark, DE 19713
} (302) 366-0500 ext 2812
}
}
} -----Original Message-----
} } From: jim [mailto:jim-at-proscitech.com.au]
} Sent: Thursday, September 14, 2000 9:53 PM
} To: 'Ritchie Sims'; Ekstrom, Harry; Microscopy-at-sparc5.microscopy.com
} Subject: RE: Carbon Evaporation Units
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
}
} --------------------------------------------------------------
} ---------.
}
}
} Retch is right, but you could also have a poor cooling system
} on that pump,
} or
} just a badly designed system. In either case backstreaming of
} oil vapour
} would
} lead to poorly adhearing films.
} Cheers
} Jim Darley
} ProSciTech Microscopy PLUS
} PO Box 111, Thuringowa QLD 4817 Australia
} Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
} Great microscopy catalogue, 500 Links, MSDS, User Notes
} ABN: 99 724 136 560 www.proscitech.com
}
} On Friday, September 15, 2000 6:55 PM, Retch Sims
} [SMTP:r.sims-at-auckland.ac.nz]
} wrote:
} }
} }
} }
} } }
} } } We currently have a Bench Top Turbo carbon evaporation
} unit made by
} Denton.
} } } We are very displeased with it. We consistently get non uniform
} coatings
} } } that peel off of our metallographic mounts. Any suggestions on a
} } } evaporation unit that works well or any information on
} 'sputtering"
} carbon?
} } } Any other experiences out there?
} } }
} }
} } I would look very carefully at the cleanliness of the samples before
} } coating.
} } I use methanol then Freon then a dry Kimwipe, and the carbon
} } coating is tenacious on brass.
} }
} } cheers
} }
} } rtch
} }
} }
} } Ritchie Sims Phone : 64 9 3737599 ext 7713
} } Department of Geology Fax : 64 9 3737435
} } The University of Auckland email : r.sims-at-auckland.ac.nz
} } Private Bag 92019
} } Auckland
} } New Zealand
}
}



From daemon Mon Sep 18 10:24:02 2000



From: =?iso-8859-1?q?Jeremy=20Sanderson?= :      jb_sanderson-at-yahoo.com
Date: Mon, 18 Sep 2000 08:08:35 -0700 (PDT)
Subject: LM condenser wanted

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear listserver,

I am trying to locate a Zeiss
(Oberkochen)phase-contrast condenser for their 160mm
tubelength classic grey series of microscopes.

The phase-contrast condenser needs to have
achromatic-aplanatic top lenses, for the phase ring
inserts that I have. I am prepared to buy any
condenser that i can locate. Can anyone out there help
me?

(I hope that this is the right place to post this
request, Nestor)

Please reply privately to me on jb_sanderson-at-yahoo.com

Regards,
Jeremy Sanderson



__________________________________________________
Do You Yahoo!?
Yahoo! Mail - Free email you can access from anywhere!
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From daemon Mon Sep 18 11:16:09 2000



From: Ronald Vane :      RVaneXEI-at-concentric.net
Date: Sunday, September 17, 2000 6:29 PM
Subject: RE: Oil backstreaming & TMPs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Ritchie and List

Basically a distillation process allows roughing pump oil to leak through
the diffusion pump. The rough pump oil boils up through the top jet of the
diff. pump where as a lighter weight component it may collide with another
molecule and bounce up into the chamber and backstream. The heavier
diffusion pump oil is less likely to lose its downward and forward momentum
during a collision.

Ron Vane
XEI Scientific
Redwood City, CA 94061
650-369-0133


-----Original Message-----
} From: Ritchie Sims {r.sims-at-auckland.ac.nz}
To: Wil Bigelow {bigelow-at-engin.umich.edu} ; microscopy-at-sparc5.microscopy.com
{microscopy-at-sparc5.microscopy.com}


Thank you Wil

Is there a corresponding picture for a diff pump ie why do they let
RP oil through?
That's assuming, of course, that the oil that builds up on eds
windows IS RP oil. It is, isn't it?

cheers

rtch


}
} The reason that you can back up a turbomolecular pump with an oil-sealed
} rotary vane pump and still have a system that is virtually free of oil
} contamination is that the compression ratio of turbo pumps varies
} exponentially with the square root of the molecular mass of the molecules
} being pumped. Thus, for nitrogen, which has M = 28, this value is about
} 200, whereas for a hydrocarbon molecule such as octane with M = 66 it is
} greater than 3000, and for a molecule of pump oil with M greater than 100
} it is greater than a million. These values are for a single compression
} stage (rotor disk) of a turbo pump. Actual turbo pumps have from 15 to 20
} such stages which act in series, so the overall value for nitrogen ends up
} being around 10^9, while for hydrocarbons it becomes astronimical (10^40
is
} often cited). Thus, if a turbo pump that is backed by a rotary vane pump
is
} managed so that the rotor never drops below about 75% of full operating
} speed while the pressure in it is in the molecular flow range (below about
} 1 Torr is a safe figure), it is virtually impossible for oil molecules to
} backstream through it. These matters are all discussed in detail on pages
} 229 to 234 of my book 'Vacuum Methods in Electron Microscopy' (see
} http:/www.2spi.com/catalog/books/book48.html, and
} http:/pup.princeton.edu/titles/6484.html for a description) and
} appropriate operating protocols are are described on pp. 249 to 253 .
}
} Wilbur C. Bigelow, Prof. Emeritus
} Materials Sci. & Engr., University of Michigan
} Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu;
} Fx:734-763-4788; Ph:734-662-5237
}
}
}
}
Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand






From daemon Mon Sep 18 12:38:35 2000



From: Richard Duerst :      richard.w.duerst-at-abbott.com
Date: Mon, 18 Sep 2000 12:21:12 -0500
Subject: FT-IR

Contents Retrieved from Microscopy Listserver Archives
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Martin,

The Nicolet phone number is 9-1-800-642-6538. If they can't help, please
call me at 847-937-7130.

Richard



From daemon Mon Sep 18 13:10:03 2000



From: Rick Harris :      raharris-at-ucdavis.edu
Date: Mon, 18 Sep 2000 10:52:03 -0700
Subject: Re: Dye Sub Printer Speeds

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



}
} My question is: how fast should this sort of printer print when using the
} parallel cable to feed data to it? Is 5 minutes / 1MB unusually slow? I
} get frustrated particularly as my computer is locked up while the data is
} being sent out and I cannot continue imaging until the data dump is done. I
} have the Win 95 spooler running of course and I don't have this problem with
} my HP 970 inkjet which I use normally.

Richard,

I have the immediate predecessor to the 8670, the 8650. We have 64 meg ram
in the printer. We have the ethercard connector because we were told it
printed faster. There is a user sitting at the printer workstation right
now printing a 14 meg image. It is taking about 35 seconds for Photoshop
to process the file and export to our print server. The print server sends
the image to the printer and it is taking 90 seconds to print. The NT
Workstation with Photoshop is a 3 year old dual Pentium Pro with 512 meg
ram. The Photoshop preferences are set to 3 scratch disks on an unused 9
gig HDD on the same machine. The NT print server is a Pentium II 400 with
256K ram. Postscript takes longer. Very large files take longer (sizes 30
meg and up). The delay occurs in Photoshop processing and between color
panels once printing has started and for very large files the time can be
as long as several minutes. B&W 1 meg files will print in 70 seconds.





Rick A. Harris, Director
Microscopy and Imaging Facility
Section of Molecular and Cellular Biology
1241 Life Sciences Addition
University of California
Davis, CA
530 752 2914
530 754 7536 fax
http://katie.ucdavis.edu
raharris-at-ucdavis.edu




From daemon Mon Sep 18 14:22:20 2000



From: Tom Phillips :      PhillipsT-at-missouri.edu
Date: Mon, 18 Sep 2000 13:46:34 -0500
Subject: % of antibodies that work in immuno

Contents Retrieved from Microscopy Listserver Archives
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Lots of antibodies don't work at LM immunocytochemistry and even more
don't work at the EM level. I would like to make an informal survey.
If you respond to me or the list, I will post a summary to the list.

Here are the questions:

Have you generated antibodies that work in some method (ELISA,
Westerns, immunoprecipitation) but not LM immunocytochemistry?

Were they monoclonals or polyclonals?

What percentage of the antibodies you have made work at the LM level
but not for EM immunocytochemistry?

If you have made antibodies against synthetic peptides, what
percentage work in either LM or EM?

Thanks, Tom

--
Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)



From daemon Mon Sep 18 16:24:53 2000



From: Tamara Howard :      howard-at-cshl.org
Date: Mon, 18 Sep 2000 17:03:40 -0400 (EDT)
Subject: Re: % of antibodies that work in immuno

Contents Retrieved from Microscopy Listserver Archives
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This is going to be a *very* depressing survey.......Maybe you should
include a salary survey and get all the glum stuff out of the way at one
time?

Tamara Howard
CSHL

On Mon, 18 Sep 2000, Tom Phillips wrote:

} Lots of antibodies don't work at LM immunocytochemistry and even more
} don't work at the EM level. I would like to make an informal survey.
} If you respond to me or the list, I will post a summary to the list.
}
} Here are the questions:
}
} Have you generated antibodies that work in some method (ELISA,
} Westerns, immunoprecipitation) but not LM immunocytochemistry?
}
} Were they monoclonals or polyclonals?
}
} What percentage of the antibodies you have made work at the LM level
} but not for EM immunocytochemistry?
}
} If you have made antibodies against synthetic peptides, what
} percentage work in either LM or EM?
}
} Thanks, Tom
}
} --
} Thomas E. Phillips, Ph.D.
} Associate Professor of Biological Sciences
} Director, Molecular Cytology Core Facility
}
} 3 Tucker Hall
} Division of Biological Sciences
} University of Missouri
} Columbia, MO 65211-7400
} (573)-882-4712 (voice)
} (573)-882-0123 (fax)
}






From daemon Mon Sep 18 17:19:06 2000



From: Ronald Vane :      RVaneXEI-at-concentric.net
Date: Mon, 18 Sep 2000 14:56:21 -0700
Subject: OIl on Detector Windows

Contents Retrieved from Microscopy Listserver Archives
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One more note on the oil found on EDS detector windows. It isn't always
roughing pump oil. Rick Felten found through IR analysis that the
plasticizer from the vacuum hose connecting the roughing pump to the diff
pump was at fault. Black vacuum hose from Japan is a bad actor in this way.
The Red Vacuum Hose made in the USA has less problems.

} Ron Vane
} XEI Scientific
} www.SEMCLEAN.COM





From daemon Mon Sep 18 17:34:53 2000



From: Ronald Vane :      RVaneXEI-at-concentric.net
Date: Mon, 18 Sep 2000 17:14:51 -0500
Subject: OIl on Detector Windows

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


One more note on the oil found on EDS detector windows. It isn't always
roughing pump oil. Rick Felten found through IR analysis that the
plasticizer from the vacuum hose connecting the roughing pump to the diff
pump was at fault. Black vacuum hose from Japan is a bad actor in this way.
The Red Vacuum Hose made in the USA has less problems.

Ron Vane
XEI Scientific
www.SEMCLEAN.COM





From daemon Mon Sep 18 19:42:26 2000



From: Oscar Jinsin :      bknp-at-nandomail.com
Date: Mon, 18 Sep 2000 18:18:43 -0500
Subject: Your Chance #15E1

Contents Retrieved from Microscopy Listserver Archives
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From daemon Mon Sep 18 23:45:06 2000



From: Paul Webster :      pwebster-at-hei.org
Date: 18 Sep 00 21:28:44 -0700
Subject: RE: % of antibodies that work in immuno

Contents Retrieved from Microscopy Listserver Archives
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Reply to: RE: % of antibodies that work in immuno
Dear Tom,

Why bother? It is obvous that some antibodies will only work with a specific technique. Why this happens is still open to speculation, but it is most likely due to masking of antigenic sites. If an antibody is made that recognises a protein only when it is configured in its natural state then the antibody will not work by Western blot. It may work really well after the sample has been fixed in high aldehyde concentration (or after osmium fixation!) as long as it has been fixed in its "natural" configuration. I wonder how many really good antibodies were discarded because they did not label denatured protein on a Western?

If the fixation process is so efficient that other proteins are retained around the antigenic site, then the antibody will not work either.
Maybe the antibody has been diluted in inappropriate blocking agent and does not work for EM when it worked for LM. One example I know of occurred when the researcher was using rabbit serum as a blocking agent. Everything worked well by LM but at the EM level, when he used protein A-gold, there was no signal. He diluted the gold probe in the rabbit serum which bound very efficiently to the gold and stopped it from binding to the antibody on the section.

Monoclonal antibodies are more specific than polyclonals, and anti-peptide antibodies are usually even more specific than many monoclonals. If the antigenic sequence is contained inside a molecule then is may become inaccessible after good fixation. If the antigen is a small peptide chain that can be easily dislodged, then it may be washed away duing the fixation or labeling process. Labeling success will depend on the preparation protocols used and each will be usiue for each antibody-antigen system.

All of these problems have solutions available to fix them. Saying an antibody works for one method but not another only serves to re-enforce the "black magic" aspect of our VERY sceintific discipline. All these statements really mean is that we either do not fully understand our preparation and labeling protocols, or that we have no idea what our antibodies are binding to.
Regards,

Paul Webster

Paul Webster, Ph.D.
Scientist II & Director
Ahmanson Advanced Electron Microscopy & Imaging Center
House Ear Institute
2100 West Third St.
Los Angeles, CA 90057

Phone: (213) 273-8026
Fax: (213) 413-6739
e-mail: pwebster-at-hei.org
http://www.hei.org/htm/aemi.htm




From daemon Tue Sep 19 03:07:37 2000



From: Lauran Oomen :      Oomen-at-nki.nl
Date: Tue, 19 Sep 2000 09:45:48 +0200
Subject: LM - Need Optical Density Standard: Summary

Contents Retrieved from Microscopy Listserver Archives
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First of all I would like to thank all of you who responded to my
question on standards for measuring optical density in transmission
light microscopy.
As a service to the "list" (at least I hope it is) I post a summary of
the responses I have got. Most of them were 'off line'.
Different types of ND filters can be obtained from:
1. Edmund Industrial optics
(http://www.edmundoptics.com/IOD/DisplayProduct.cfm?Productid=1945)
A range of mounted or unmounted glass filters (ND 0.15-2.5) and a
stepped one with 11 discrete density steps (0.1 steps from ND 0.04 to
1.0)
2. Lee Filters (http://www.leefilters.co.uk/)
A range of unmounted filters (ND 0.1-0.9) and as a set of three (ND 0.3,
0.6 and 0.9) (material not specified, but might be available as resin or
gelatin filters, according to the representative in the Netherlands)
3. Kodak
(http://www.kodak.com/country/US/en/motion/support/h1/filtration.shtml#camfilm)

A range of unmounted filters (gelatin WRATTEN; ND 0.1-4.0)
4. Cargill Industries in NJ
Not been able to find info (yet)
5. Densichron
Not been able to find info (yet)


--
****************************************************
Lauran C.J.M. Oomen oomen-at-nki.nl
The Netherlands Cancer Institute tel. +31 20 5121889
Digital Microscopy Facility fax. +31 20 5121893
Plesmanlaan 121
1066CX Amsterdam
The Netherlands
****************************************************





From daemon Tue Sep 19 06:46:25 2000



From: Frank Thomas :      thomasf-at-AGC.BIO.NS.CA
Date: Tue, 19 Sep 2000 08:25:32 -0300
Subject: ESEM beam problem

Contents Retrieved from Microscopy Listserver Archives
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Listers-

Our ESEM has developed a problem with its beam. I don't know if many other
instruments have this, but there is a "source" mode button which , when
hit, provides a sort of view "back up the column towards the gun". It's
used for saturating the filament - when properly saturated, the beam should
look like a nice round to oval bright spot. Our instrument has a LaB6
source, which, because of the shape of the tip of the crystal, may produce
four weaker "satellite" beams around the "main" beam - which is OK, you
just want to be sure you're using the main beam.
Just yesterday, and quite suddenly, this view changed somewhat - we no
longer seem to have a nice oval spot, but instead a sort of cross shape,
with one side smeared out into a separate, smaller beam. Signal has been
dramatically reduced - I have to open up the condensor 'way more than I
should, to get any kind of reasonable signal, and then the resolution is
pretty degraded.
I'm thinking the LaB6 has failed, perhaps with some small bit falling off
one side. Previously, when one of these has died on us, it's pretty sudden
and dramatic - suddenly no signal, period. Our gun vacuum is generally
pretty good, but we have at least a thousand hours on this filament, so I
suppose it may be on its last legs.
Assuming I'm right in blaming it on the LaB6 - I can't think of any other
reason for this problem - has anyone else had a filament fail in this way?
I'd just like to hear of any other possible causes before I open up the
gun. I suppose it's possible some little bit of crud found its way
partially up the column and is causing severe astigmatism, but that sounds
like a major disassembly effort, too.

F.C. Thomas
MicroAnalysis Facility
Bedford Institute of Oceanography
Dartmouth, Nova Scotia
Canada
Geological Survey of Canada (Atlantic)
Bedford Institute of Oceanography
Dartmouth, Nova Scotia
Canada B2Y 4A2



From daemon Tue Sep 19 08:09:42 2000



From: Shalvoy, Richard B **CHES :      RBShalvoy-at-archchemicals.com
Date: Tue, 19 Sep 2000 07:50:28 -0500
Subject: Dye Sub printer speeds- followup

Contents Retrieved from Microscopy Listserver Archives
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Thanks to all who responded with my question about slow printing from my
Kodak dye sub printer. I have tried some things and have tracked the
problem down some.

I found I can print via the parallel port to the printer just fine from a
second computer (Dell 450) in the lab, using freshly reinstalled postscript
drivers. A 1.2MB file prints in about 1 minute and the transfer to the
printer (Progress in the Systray Printer icon box) goes in a few seconds.
In turn the same file, same cable, same software, both win 95, but
different computer (Compaq 333 attached to my Hitachi SEM) takes 4.5 minutes
to get over the printer, before printing starts, transferring in blocks of
about 60kB, 1 every 8-10 seconds. I am not logged onto the network, and
have disabled my Norton anti-virus software.

This experiment rules out a lot of issues and now I'm focusing on the setup
of my SEM computer to see why the Progress goes so slowly on it while it
goes so much faster on the Dell. If anyone has ideas about THIS issue I'll
gladly try them out. Maybe Hitachi's computer guy has some ideas.

Richard Shalvoy
---------------------
original note

I have a Kodak 8670 dye sub printer hooked up to my SEM, but it seems to me
that it takes a long time to get it to print and also while it is in the
printing process my computer (and hence my SEM) is locked up. Here are the
details.

I have the printer (1 year old) hooked to a PC with a 333 Pentium II
processor (128 MB RAM) using a parallel cable. I do get the same
performance when printing from other computers so I doubt that it is
anything peculiar to this one computer.

Now when I call for a printout it takes 5 minutes to get the 1MB file size
print done, and almost all of the time is spent sending data to the printer
- the actual printing takes a short time (30 sec). I have discussed this
with Kodak Tech Support and they claim my performance is not unusual.
HOWEVER, I also experienced much quicker printing times (I'm sure - I have
notes, but it was a while ago) when I was first using the printer (1 minute
/ 1 MB of file size in the image) and this is confusing me.

My question is: how fast should this sort of printer print when using the
parallel cable to feed data to it? Is 5 minutes / 1MB unusually slow? I
get frustrated particularly as my computer is locked up while the data is
being sent out and I cannot continue imaging until the data dump is done. I
have the Win 95 spooler running of course and I don't have this problem with
my HP 970 inkjet which I use normally.

I will welcome any good ideas or cold realities that apply.

Richard Shalvoy
Arch Chemicals
350 Knotter Drive
Cheshire, CT 06410
203-271-4394



From daemon Tue Sep 19 08:21:53 2000



From: Anthony Garratt-Reed :      tonygr-at-mit.edu
Date: Tue, 19 Sep 2000 09:05:25 -0400
Subject: Re: Antivibrition platforms

Contents Retrieved from Microscopy Listserver Archives
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Vladimir-

The XL30 FEG-ESEM is already mounted on a built-in air-supported
anti-vibration system. Before you go ahead and specify (or buy) an air
table, I would suggest you look very carefully at the resonances, and how
they would be affected by the additional isolation. Things don't always
get better - in fact, an air table could make your situation worse if the
resonances are not carefully thought out.

At the very least, have a professional vibration analysis performed to show
what vibrations are present at your site and how the built-in system is
responding.

I don't know about the CM12 and how it is supported.

Tony Garratt-Reed.


}
} Hi All,
} I beg your pardon for bringing up this issue again, but
} when I did not need it, I did not pay attention to postings...
}
} Now I have to include in a construction grant a price for
} antivibration table for FEG-ESEM XL30 and, possibly,
} for STEM CM12. In XL30 I have problems with vibrations
} at magnifications 100-200k (and sometimes starting at 50k).
}
} Who got good results with antivibrition platforms? What
} type? Should only a column be isolated or a whole instrument?
} What is a probable price range (including installation)?
}
} Thanks,
}
} Vladimir
}


** Anthony J. Garratt-Reed
** MIT Room # 13-1027
** 77 Massachusetts Avenue
** Cambridge, MA 02139-4307
** USA
**
** Phone: (+) 1-617-253-4622
** Fax: (+) 1-617-258-6479
**





From daemon Tue Sep 19 09:05:36 2000



From: Roger Moretz :      rcmoretz-at-excite.com
Date: Tue, 19 Sep 2000 06:49:03 -0700 (PDT)
Subject: Re: TEM tissue culture melinex film

Contents Retrieved from Microscopy Listserver Archives
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Carole:

My use of Alar was a veerrryyy looong time ago, but, my recall is that the
use of the warm fixative was more to fix the cells at the same temperature
as the culture medium. This is due to the fact that many of the cells
contract/round up/do nasty things when exposed to cold fixative. If you are
concerned about membrane mobility artifacts, I would have thought that using
a fixative that is the same temperature as the culture medium would be most
appropriate. However, it might be useful to compare cells fixed at 37 and 4
C for your experiment. I know that this adds another layer of complexity
onto the experimental design, but if this is a major issue, then I think it
should be done. Let us know how it turns out.

Roger Moretz, Ph.D.
Dept of Toxicology
Boehringer Ingelheim Pharmaceuticals
On Mon, 18 Sep 2000 11:44:12 +0100, Carole Elleman wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear All has anyone any experience with Agar's melinex film for growing
} cells on before fixation and embedding for the TEM? I would like to find
out
} why it is necessary to fix the film with warm fixative - doesn't this
} introduce some artefacts due to membrane mobility at 37 degrees? I am
} looking at membrane antigens!
} Carole Elleman
}
}





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From daemon Tue Sep 19 10:41:30 2000



From: Cleaver, Gordon :      GCleaver-at-schafercorp.com
Date: Tue, 19 Sep 2000 11:11:32 -0400
Subject: Salaries

Contents Retrieved from Microscopy Listserver Archives
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Dear Lucille:

As a coincidence, one of my technologists pulled your request for survey
response off the web to tell me that I was under paying him. I presume that
your classification of "engineer" refers to technical degree level people. I
manage a group of 6 technical people with about ten state of the art level
SEM's, TEM, EMP's and XRD equipment for a very long term AFTAC contract.
AFTAC HQ is at Patrick AFB. Because we reside near Silicon Valley (just
West of LLNL) we must reach deeper for starting rates here where entry
level housing is about $300K. We cannot compete with the high tech firms
that offer stock options, signing bonuses, etc. I just lost a materials
scientist who about doubled her salary at a firm in Fremont. Obviously, our
starting rates are a little higher than you indicate for
engineers/scientists as well as technicians with little difference for
degree status. I don't think that we have started anyone in our
organization lower than the mid $40's in the past couple of years.
Otherwise, we work with a similar salary range for non senior level
professionals without significant supervisory responsibility.

Good luck,

Gordon





From daemon Tue Sep 19 12:23:04 2000



From: Larry D. Ackerman :      mishot-at-itsa.ucsf.edu
Date: Tue, 19 Sep 2000 09:35:50 -0700
Subject: Drosophila sectioning

Contents Retrieved from Microscopy Listserver Archives
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Since no one has responded to this question in the past month I will
provide my perspective. I have not seen any epoxy that will adhere to
chitinous structures during sectioning. If someone else has please post the
information. What I typically do when sectioning the eyes is to cut ten two
micrometer sections on a dry glass knife then pick up the tissue (discard
the piece of surrounding epoxy) with a fine needle probe and transfer it to
a drop of water on a glass microscope slide. When I have enough sections
the slide is moved to a hot plate for drying and later staining with
toluidine blue. This works for the eye because epoxy infiltrates the
underlying tissue from the backside. The proboscis is cut away and
sometimes the head is cut in half during fixation. For fixing and
infiltrating legs I would expect that this technique would work if they are
cut in order to provide an opening at each end for fluid transfer. Best wishes.


Larry D. Ackerman
Lily & Yuh Nung Jan Laboratories
Howard Hughes Medical Institute
UCSF, Box 0725, Rm U226
533 Parnassus Ave.
San Francisco, CA 94143

(415) 476-8751 FAX (415) 476-5774
mishot-at-itsa.ucsf.edu



From daemon Tue Sep 19 13:15:04 2000



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Wed, 20 Sep 2000 06:05:33 GMT+1200
Subject: Re: Dye Sub printer speeds- followup

Contents Retrieved from Microscopy Listserver Archives
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When you solve this problem, I'd be really interested to hear what it
was.
Printers drive me nuts, too.

rtch



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}
}
} Thanks to all who responded with my question about slow printing from my
} Kodak dye sub printer. I have tried some things and have tracked the
} problem down some.
}
} I found I can print via the parallel port to the printer just fine from a
} second computer (Dell 450) in the lab, using freshly reinstalled postscript
} drivers. A 1.2MB file prints in about 1 minute and the transfer to the
} printer (Progress in the Systray Printer icon box) goes in a few seconds.
} In turn the same file, same cable, same software, both win 95, but
} different computer (Compaq 333 attached to my Hitachi SEM) takes 4.5 minutes
} to get over the printer, before printing starts, transferring in blocks of
} about 60kB, 1 every 8-10 seconds. I am not logged onto the network, and
} have disabled my Norton anti-virus software.
}
} This experiment rules out a lot of issues and now I'm focusing on the setup
} of my SEM computer to see why the Progress goes so slowly on it while it
} goes so much faster on the Dell. If anyone has ideas about THIS issue I'll
} gladly try them out. Maybe Hitachi's computer guy has some ideas.
}
} Richard Shalvoy
} ---------------------
} original note
}
} I have a Kodak 8670 dye sub printer hooked up to my SEM, but it seems to me
} that it takes a long time to get it to print and also while it is in the
} printing process my computer (and hence my SEM) is locked up. Here are the
} details.
}
} I have the printer (1 year old) hooked to a PC with a 333 Pentium II
} processor (128 MB RAM) using a parallel cable. I do get the same
} performance when printing from other computers so I doubt that it is
} anything peculiar to this one computer.
}
} Now when I call for a printout it takes 5 minutes to get the 1MB file size
} print done, and almost all of the time is spent sending data to the printer
} - the actual printing takes a short time (30 sec). I have discussed this
} with Kodak Tech Support and they claim my performance is not unusual.
} HOWEVER, I also experienced much quicker printing times (I'm sure - I have
} notes, but it was a while ago) when I was first using the printer (1 minute
} / 1 MB of file size in the image) and this is confusing me.
}
} My question is: how fast should this sort of printer print when using the
} parallel cable to feed data to it? Is 5 minutes / 1MB unusually slow? I
} get frustrated particularly as my computer is locked up while the data is
} being sent out and I cannot continue imaging until the data dump is done. I
} have the Win 95 spooler running of course and I don't have this problem with
} my HP 970 inkjet which I use normally.
}
} I will welcome any good ideas or cold realities that apply.
}
} Richard Shalvoy
} Arch Chemicals
} 350 Knotter Drive
} Cheshire, CT 06410
} 203-271-4394
}
}
Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand



From daemon Tue Sep 19 14:37:17 2000



From: Timothy Schneider :      Timothy.Schneider-at-Mail.TJU.EDU
Date: Tue, 19 Sep 2000 15:16:26 -0400
Subject: Re: Immuno TEM

Contents Retrieved from Microscopy Listserver Archives
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Hello:

I work in a lab that has great success with immuno labeling on a fluoresce
laser confocual level, but taking the same antibodies to TEM thin sections
is generally a lost cause. My success rate is probably around 5 to 10% (and
that's a lot higher then my annual raises). Tim

Timothy G. Schneider
Director of Electron Microscopy
Department of Pathology
Room 229 Jefferson Hall
Thomas Jefferson University
1020 Locust St.
Philadelphia Pa. 19107
215-503-4798 work
610-613-8170 cellular
timothy.schneider-at-mail.tju.edu




From daemon Tue Sep 19 14:42:58 2000



From: Alan Burns :      aburns-at-bcm.tmc.edu
Date: Tue, 19 Sep 2000 14:16:25 -0500
Subject: SEM need help on endothelial monolayers

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I am having trouble obtaining good SEM preparations of cultured endothelial
cell monolayers. The monolayers show evidence
of artefactual cracking along cell borders. The cracks appear whether the
cells are grown on glass coverslips or transwell filters. I have tried
various
fixatives (buffered glutaraldehyde alone, glutaraldehyde followed by osmium
and uranyl acetate), different dehydrating agents (ethanol or acetone),
followed by critical point drying (CPD). I have even tried
tetramethylsilane (Ted Pella) in place of CPD. The only time I get good
images (i.e.,
no cracks) is when a small portion of the monolayer has inadvertently
detached from its substrate during tissue processing. The cells on this
small flap look marvelous (no cracks). I believe, in the absence of
substrate adhesion, the cells on the flap shrink uniformly during dehydration
when surface tension forces exert their effects. Does anyone know how to
prepare cell monolayers for SEM that leaves them intact and free
from artefactual cracking?

Thanks.

Alan Burns, Ph.D.
Assistant Professor
Cardiovascular Sciences
Department of Medicine
Baylor College of Medicine



From daemon Tue Sep 19 14:45:14 2000



From: Rick Harris :      raharris-at-ucdavis.edu
Date: Tue, 19 Sep 2000 12:30:29 -0700
Subject: Re: Dye Sub printer speeds-a suggestion

Contents Retrieved from Microscopy Listserver Archives
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This relates to the question of printing from the computer that runs the SEM.

It is my policy in our facility that computers that operate microscopes
such as our Hitachi S3500N and our confocals are not used for any other
purpose. Images acquired are not saved on the computer that operates the
device but rather are saved to a network drive (on a server) that is mapped
to the individual user. All images from all devices go to the same
server. The function of the server is to distribute these images, via
direct connection, http, ftp to the individual users. The computers that
run the devices are thus never clogged with images and are free of any
programs other than those needed to run the device. The computers run
faster and have fewer failures. They are setup the same as the day they
were installed except for the network connection. I have often seen
computer support personnel make the mistake of assuming that they can
troubleshoot a computer than runs an SEM or confocal or other high end
device only to discover that these computers are integral parts of the
imaging device and should only be repaired by qualified technicians
familiar with the device, not the computer.

Whenever possible, I suggest isolating the computer that drives your
microscope from any other function.


Rick A. Harris, Director
Microscopy and Imaging Facility
Section of Molecular and Cellular Biology
1241 Life Sciences Addition
University of California
Davis, CA
530 752 2914
530 754 7536 fax
http://katie.ucdavis.edu
raharris-at-ucdavis.edu




From daemon Tue Sep 19 16:41:15 2000



From: Spaulding, Robert F :      SpauldinRF-at-corning.com
Date: Tue, 19 Sep 2000 17:31:45 -0400
Subject: SEM - submicron width measurements

Contents Retrieved from Microscopy Listserver Archives
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I am looking for resources describing the accurate measurement of micron and submicron sized features in the SEM.

Thanks,

spauldinrf-at-corning.com



From daemon Tue Sep 19 16:41:17 2000



From: Earl Weltmer :      earlw-at-pacbell.net
Date: Tue, 19 Sep 2000 14:22:50 -0700
Subject: Re: ESEM beam problem

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Yes, the LaB6 has failed.

Earl

Frank Thomas wrote:

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} -----------------------------------------------------------------------.
}
} Listers-
}
} Our ESEM has developed a problem with its beam. I don't know if many other
} instruments have this, but there is a "source" mode button which , when
} hit, provides a sort of view "back up the column towards the gun". It's
} used for saturating the filament - when properly saturated, the beam should
} look like a nice round to oval bright spot. Our instrument has a LaB6
} source, which, because of the shape of the tip of the crystal, may produce
} four weaker "satellite" beams around the "main" beam - which is OK, you
} just want to be sure you're using the main beam.
} Just yesterday, and quite suddenly, this view changed somewhat - we no
} longer seem to have a nice oval spot, but instead a sort of cross shape,
} with one side smeared out into a separate, smaller beam. Signal has been
} dramatically reduced - I have to open up the condensor 'way more than I
} should, to get any kind of reasonable signal, and then the resolution is
} pretty degraded.
} I'm thinking the LaB6 has failed, perhaps with some small bit falling off
} one side. Previously, when one of these has died on us, it's pretty sudden
} and dramatic - suddenly no signal, period. Our gun vacuum is generally
} pretty good, but we have at least a thousand hours on this filament, so I
} suppose it may be on its last legs.
} Assuming I'm right in blaming it on the LaB6 - I can't think of any other
} reason for this problem - has anyone else had a filament fail in this way?
} I'd just like to hear of any other possible causes before I open up the
} gun. I suppose it's possible some little bit of crud found its way
} partially up the column and is causing severe astigmatism, but that sounds
} like a major disassembly effort, too.
}
} F.C. Thomas
} MicroAnalysis Facility
} Bedford Institute of Oceanography
} Dartmouth, Nova Scotia
} Canada
} Geological Survey of Canada (Atlantic)
} Bedford Institute of Oceanography
} Dartmouth, Nova Scotia
} Canada B2Y 4A2




From daemon Tue Sep 19 16:45:03 2000



From: Mark Riggs :      riggsm-at-svg.com
Date: Tue, 19 Sep 2000 17:16:59 -0400
Subject: thermal paper needed

Contents Retrieved from Microscopy Listserver Archives
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standard source for thermal paper for seikosha and sony thermal printers has been vital image technology. are there other vendors out there?

mark riggs
sem metrology
svg lithography
wilton, ct




From daemon Tue Sep 19 18:39:40 2000



From: Lisa Cook :      llcook-at-julian.uwo.ca
Date: Tue, 19 Sep 2000 18:29:59 -0500
Subject: Adherence of Lyophilized Protein to Slides

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Subscribe Microscopy: LLCOOK-at-julian.uwo.ca

I am trying to create a positive control for a immunohistochemistry
protocal. I have lyophilized protein which I have reconstituted in PBS.
Does anyone have any ideas how I can adhere this protein to a slide so that
it can be stained with the normal histo protocal.

I have tried drying the solution in the oven at 37C for different lengths
of time. Should I use a gelatin solution.

Lisa Cook

LLCOOK-at-julian.uwo.ca





From daemon Tue Sep 19 19:04:00 2000



From: Elaine Humphrey :      ech-at-unixg.ubc.ca
Date: Tue, 19 Sep 2000 05:24:35 -0700
Subject: Re: SEM need help on endothelial monolayers

Contents Retrieved from Microscopy Listserver Archives
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Frank,
Sounds like on of two things - either it failed that way because of the
amount of hours(as you suggested), or, the tip was brought up to
"saturation" way to fast.

Gary M. Easton
Scanners Corporation
Third Party EM Service
----- Original Message -----
} From: Frank Thomas {thomasf-at-AGC.BIO.NS.CA}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Tuesday, September 19, 2000 7:25 AM


Hi Alan
We also had this problem but solved it by using tannic acid and
making sure the cells were never fully uncovered.

There are several ways of using tannic acid.

Fix: 2.5% glutaraldehyde in 0.1M cacodylate buffer pH 7.3-7.4 30 minutes

I use two plastic pipettes - as I remove the liquid from the bottom
of the culture dish, I add the new liquid to the top always making
sure there is a thin layer of liquid on the cells. Three fillings of
the chambers at each change ensures a good exchange.

Wash: 0.1M cacodylate buffer 3x5minutes

Postfix: 1%osmium tetroxide in 0.1M cacodylate buffer 30 minutes
1% tannic acid in 0.1M cacodylate buffer 30-60 minutes
1%osmium tetroxide in 0.1M cacodylate buffer 30 minutes

Rinse: ddw

Dehydrate: 5 minutes each in 30%, 50%, 70%, 85%, 95%, 100%, 100%, 100%

CPD.

If you remove all the liquid at a change of solutions, there can be
damage to cellular processes such as filopodia. The tannic acid acts
as a mordant and makes a real difference to the surface of the cells.
I think this method is called the OTO method (Osmium, Tannic Acid,
Osmium).

I have also used Tannic Acid in the buffer wash and then just used
Osmium as normal. That worked too and was less expensive on Osmium.
If you have empty wells when you use the OTO method, you can take off
the first osmium and put it into an adjacent well. Then after the
tannic acid, put the same osmium back.

Elaine



}
} I am having trouble obtaining good SEM preparations of cultured endothelial
} cell monolayers. The monolayers show evidence
} of artefactual cracking along cell borders. The cracks appear whether the
} cells are grown on glass coverslips or transwell filters. I have tried
} various
} fixatives (buffered glutaraldehyde alone, glutaraldehyde followed by osmium
} and uranyl acetate), different dehydrating agents (ethanol or acetone),
} followed by critical point drying (CPD). I have even tried
} tetramethylsilane (Ted Pella) in place of CPD. The only time I get good
} images (i.e.,
} no cracks) is when a small portion of the monolayer has inadvertently
} detached from its substrate during tissue processing. The cells on this
} small flap look marvelous (no cracks). I believe, in the absence of
} substrate adhesion, the cells on the flap shrink uniformly during dehydration
} when surface tension forces exert their effects. Does anyone know how to
} prepare cell monolayers for SEM that leaves them intact and free
} from artefactual cracking?
}
} Thanks.
}
} Alan Burns, Ph.D.
} Assistant Professor
} Cardiovascular Sciences
} Department of Medicine
} Baylor College of Medicine

--
Dr. Elaine Humphrey
Biosciences Electron Microscopy Facility
University of British Columbia
6270 University Blvd, mail-stop Botany
Vancouver, BC
CANADA, V6T 1Z4
Phone: 604-822-3354
FAX: 604-822-6089
e-mail: ech-at-unixg.ubc.ca



From daemon Tue Sep 19 20:05:27 2000



From: Gary Gaugler :      gary-at-gaugler.com
Date: Tue, 19 Sep 2000 20:11:05 -0700
Subject: Re: Dye Sub printer speeds-a suggestion

Contents Retrieved from Microscopy Listserver Archives
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It could be that the printer port on the "slow computer" could be set up
in bios "wrong", i.e. as a standard printer port, with no bi-directional
capability. When booting up the Compaq, hit the appropriate key to enter
bios setup. Look for an "integrated peripherals" section. Then, check your
printer port selection. It should be set at least to the EPP mode or the
EPP+ECP(this mode gives the port DMA capabilities which further enhance
high speed transfers) mode. Save, and re-boot. Win95 should automatically
recognize the change and load the appropriate drivers. If that doesn't
work, upgrade the Compaq's memory by 64Mb's. Win95 spools print jobs to
memory(if available - if not, the hard drive). Also, check the printers
settings. Make sure it is set to "Spool" rather than "Print directly to
printer". Good luck!

Gary M. Easton
Scanners Corporation
Third Party SEM Service
----- Original Message -----
} From: Shalvoy, Richard B **CHES {RBShalvoy-at-archchemicals.com}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Tuesday, September 19, 2000 8:50 AM


How is this really different from what others are talking about? If the
computer has to talk to a printer, or in your case, to a network,
the path is still external. A pristine example is when the computer
saves to a local disk. Then, the disk is physically carried to
some other computer and accessed at that point. A network
interface requires system resources just as any other interface
does. Arguably, the network can take more resources than
other interfaces. It is not benign.

The method which SEM makers utilize a controlling computer
is a big variable. Some use programmed data transfers while
others use IRQs. The days of PIO are waning as PC OSs
move to NT-type systems. PIO is not supported. Furthermore,
this type of interface eats CPU bandwidth...in stark contrast
to IRQ approaches. A big problem with Wintel systems is the
lack of interrupts. It is an old and still aging architecture.
Watch for Win to change in many ways. A DOS-less
system is one of the key changes.

System management is a core function. This applies very
well to computers and computer systems. A single computer
shackled to a single unit is not a very useful application of
resources. If the computer is so inextricably tied to the
controlled system, I'd be very shy of such a system and
architecture.

If a computer is "clogged" in any respect, then I would submit
that these systems are poorly designed. The sore sticking
point is whether these systems will be or are supported in the
future. Too many computer-based products are here today,
gone tomorrow. How many folks are suffering with Win3.1 or
Win3.11 systems? Not much fun.


At 12:30 PM 9/19/00, you wrote:
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From daemon Wed Sep 20 01:56:57 2000



From: Paul Webster :      pwebster-at-hei.org
Date: 19 Sep 00 23:48:07 -0700
Subject: Re: Immuno TEM

Contents Retrieved from Microscopy Listserver Archives
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Reply to: Re: Immuno TEM
Tim Schneider writes:
I work in a lab that has great success with immuno labeling on a fluoresce
laser confocual level, but taking the same antibodies to TEM thin sections
is generally a lost cause. My success rate is probably around 5 to 10% (and
that's a lot higher then my annual raises). Tim

Hi Tim,

I look forward to seeing more details about your preparation protocols before commenting on your specific case. However, in all instances it is important that general statements regarding antibody reactivity be accompanied by specific examples of protocols. Only then can we compare antibody performance.

Here is a more general reply that I hope stimulates some thought:

As an addendum to my last posting, I will make the very bold statment that if an immuno-signal is seen by light microscopy the signal will be seen by EM.
How so? First look at the preparation protocol used for the LM preparation. Was it on unfixed, air dried, methanol-fixed cryostat sections? Maybe. It is a routine protocol for LM immunocytochemistry. Now look at the typical EM protocol. Good fixation to preserve morphology (maybe 4% formaldehyde and 0.1% gutaraldehyde), rapid dehydration because we do not want to wash away our antigens, finally embedding in LR White or maybe even freeze substitution into Lowicryl after rapid freezing and no chemical fixative. The morphology in the sections looks good, but where is the signal?

One possibility is that the signal cannot be generated because the sample has been so well fixed and embedded that the antigen, or the gold probe, or both cannot gain access. I bet if the same protocol used for LM was also used for the EM preparation, the signal would be there. If it could not, I would start looking for the smelly rat. Maybe the morphology would suffer a little, but as this isn't a problem for LM, why should this trouble us at the EM level? We only require the morphology to enable us to identify labeled structures.

Another possibility is that the signal really is there, but because it is in such low amount we discard it as background labeling. However, this may be the total amount of signal we will ever get on thin sections. A cryostat section is at least 10 microns thick. Dry this down to a thin smudge on the slide, make it totally accessible to antibodies by exposure to methanol and you have an 2-D sample with antigen concentrated down and totally accessible to antibody. Apply antibody, add a secondary fluorescent antibody (and maybe a bridging antibody to amplify the signal) and a very obvious result is obtained.

Take this same antibody and apply it to thin resin sections (the thinner the better to get good resolution) and there will be much less antigen present in the section (60nm v 10,000nm). Also the antibody or gold markers usually do not penetrate the section. This means that the only antigen available for binding will be the small fraction that is exposed on the surface of the section. If the antigen is present in small numbers anyway, then the specific signal will be low. If protein A-gold is used, this usually binds with antibody at a 1:1 ratio so it is possible that the specific signal for any section may be one gold particle over one structure on the whole section. This might be the result for this antibody.
This is easy to check. First find out how many antigens are present in the sample and work out how many should be exposed on the section surface. Labeling efficiency (the relationship between antigen number to observed signal) is usually between 5-20% (ie. if 100 antigens are available for binding only between 5 and 20 will label). From this it is possible to get an estimate of how many antibodies will bind to the section. It is often surprising that signal is detected at all.

In conclusion: I repeat - if an antibody doesn't work, then we probably do not understand enough about the antigen to design a good labeling protocol.

Also: If the antibody works for LM it WILL work for EM. Keep preparation and labeling protocols the same for both and you will enjoy much success. I don't think that any one of my collection of 100's of antibodies works only by LM. They all also work by EM. Some do not work on resin sections and some do not work if I fix cells with glutaraldehyde, but they all label at the EM level. Some only give me one gold particle for every 20 vesicles they are supposed to label, but the particle is always over one of these vesicles.
I look forward to a good discussion about this (I just finished the official writing!), but don't ask me about salaries.

Regards,

Paul Webster, Ph.D.
Scientist II & Director
Ahmanson Advanced Electron Microscopy & Imaging Center
House Ear Institute
2100 West Third St.
Los Angeles, CA 90057

Phone: (213) 273-8026
Fax: (213) 413-6739
e-mail: pwebster-at-hei.org
http://www.hei.org/htm/aemi.htm






From daemon Wed Sep 20 02:25:05 2000



From: Per =?iso-8859-1?Q?H=F6rstedt?= :      per.horstedt-at-pathol.umu.se
Date: Tue, 19 Sep 2000 09:16:52 +0200
Subject: Re: SEM need help on endothelial monolayers

Contents Retrieved from Microscopy Listserver Archives
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Alan,
problems with cells cracking when cultured as monolayers are mostly
depending on artifacts induced when dehydrating or drying the samples. The
most probable reason for your problem is shrinkage of the cells, which
gives severe tensions between your rigid support and the fragile cells.
It's very typical that you get good preservation with no shrinkage
artifacts when the monolayer has lost contact with the support, as they are
free to alter their volume without any hindrance from the support.
In my experience, fixation with 2.5 % buffered glutar aldehyde is normally
sufficient as a fixing agent.
Acetone should be avoided as the rate of dehydrating is too rapid and will
in may tissues induce uncontrolled shrinkage.
As I don't know your exact protocol for dehydration and CP-drying, I can
only suggest some hints, so what I would try is dehydrating with ethanol
followed by drying from liquid CO2. I would start dehydration in a
medium-low percentage, 60 - 65 % ethanol in water, and work through a
series of steps with 5% increase in concentration up to 100% ethanol. Time
in every step would be at least 10 - 15 minutes.
Substituting the ethanol with liquid CO2 in the CPD is critical, so if you
can flush the chamber with liquid gas with the chamber closed, give it at
least 10 minutes before closing the unit completely. Wait at least 2 hours
before another 10 minutes flush before starting the drying procedure. The
temperature has to be very slowly increased, normally it takes us 20
minutes to reach the state when the pressure can be released, and this last
step has also to be done very slowly, at least in terms of 30 minutes to
reach normal pressure.

This would be the way I'd go for any type of a cultured monolayer, and
normally works OK.
Good luck!
Yours sincerely

Per Hörstedt
Department of Medical Biosciences
Pathology
Unit for Electron Microscopy
University of Umeå
S-90187 Umeå
Sweden

phone int-46-90-7851541
fax int-46-90-7851215




From daemon Wed Sep 20 05:50:06 2000



From: White, Woody N :      Woody.N.White-at-mcdermott.com
Date: Tue, 19 Sep 2000 16:36:00 -0500
Subject: Re:Dye Sub printer speeds- followup

Contents Retrieved from Microscopy Listserver Archives
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Late comer to this thread, but... A few things to check:
Do the 2 computers (other than the Dell's slightly faster CPU) have similar
resources?
That includes system memory & bus speed, open hard drive space for virtual
memory, similar setup size constraints on swap file size (if any), similar
background programs running, similar hard drive access speeds... Humm,
can't
think of others to check presently.

Also, are both parallel ports set to the same mode in the system BIOS?

If the Hitachi system is like mine, it is using a SCSI I/O to the SEM, but I
don't believe that will seriously affect resources unless in use (not
positive).


Given a choice I would dump Dell and/or Compaq and go for a custom, generic
clone PC. I (we) had no choice on the Hitachi (Compaq), but I managed to do
so
for my IXRF EDS because of special circumstances - and have been very happy
with
the performance. I am somewhat biased (could you tell) about the proprietary
stuff the bigger manufacturers add to their PCs.
good luck... BTW, I am using an Epson 900, parallel I/O.

Woody White, McDermott Technology, Inc.
Me: http://home.att.net/~woody.white
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Thanks to all who responded with my question about slow printing from my
Kodak dye sub printer. I have tried some things and have tracked the
problem down some.

I found I can print via the parallel port to the printer just fine from a
second computer (Dell 450) in the lab, using freshly reinstalled postscript
drivers. A 1.2MB file prints in about 1 minute and the transfer to the
printer (Progress in the Systray Printer icon box) goes in a few seconds.
In turn the same file, same cable, same software, both win 95, but
different computer (Compaq 333 attached to my Hitachi SEM) takes 4.5 minutes
to get over the printer, before printing starts, transferring in blocks of
about 60kB, 1 every 8-10 seconds. I am not logged onto the network, and
have disabled my Norton anti-virus software.

This experiment rules out a lot of issues and now I'm focusing on the setup
of my SEM computer to see why the Progress goes so slowly on it while it
goes so much faster on the Dell. If anyone has ideas about THIS issue I'll
gladly try them out. Maybe Hitachi's computer guy has some ideas.

Richard Shalvoy
---------------------
original note

I have a Kodak 8670 dye sub printer hooked up to my SEM, but it seems to me
that it takes a long time to get it to print and also while it is in the
printing process my computer (and hence my SEM) is locked up. Here are the
details.

I have the printer (1 year old) hooked to a PC with a 333 Pentium II
processor (128 MB RAM) using a parallel cable. I do get the same
performance when printing from other computers so I doubt that it is
anything peculiar to this one computer.

Now when I call for a printout it takes 5 minutes to get the 1MB file size
print done, and almost all of the time is spent sending data to the printer
- the actual printing takes a short time (30 sec). I have discussed this
with Kodak Tech Support and they claim my performance is not unusual.
HOWEVER, I also experienced much quicker printing times (I'm sure - I have
notes, but it was a while ago) when I was first using the printer (1 minute
/ 1 MB of file size in the image) and this is confusing me.

My question is: how fast should this sort of printer print when using the
parallel cable to feed data to it? Is 5 minutes / 1MB unusually slow? I
get frustrated particularly as my computer is locked up while the data is
being sent out and I cannot continue imaging until the data dump is done. I
have the Win 95 spooler running of course and I don't have this problem with
my HP 970 inkjet which I use normally.

I will welcome any good ideas or cold realities that apply.

Richard Shalvoy
Arch Chemicals
350 Knotter Drive
Cheshire, CT 06410
203-271-4394



From daemon Wed Sep 20 08:44:12 2000



From: Tamara Howard :      howard-at-cshl.org
Date: Wed, 20 Sep 2000 09:29:20 -0400 (EDT)
Subject: Re: Immuno TEM

Contents Retrieved from Microscopy Listserver Archives
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Interesting post, but I have totake issue with one statement - I've read
it over and I hopeI'm not taking this out of context; please excuse me if
I have:

On 19 Sep 2000, Paul Webster wrote:

Lots of snipping.....} -----

} Maybe the morphology would suffer a little, but as this isn't a
} problem for LM, why should this trouble us at the EM level? We only
} require the morphology to enable us to identify labeled structures.
}
I depend on decent morphology to tell me that I haven't cause
redistribution of the antigen/mRNA/gene at some time during the
processing. If the sample looks like mush but has nice label, how am I to
know that the label is where it was in vivo? I realize this may be
extreme, to assume that everything is going to move if not properly fixed,
but I think that it critical to keep this possibility in mind when
localizing anything, at any level of detection.

My $0.02 this morning;hope I haven't ticked anyone off!

Tamara Howard
CSHL
(NY, USA for those of you who wonder!)




From daemon Wed Sep 20 09:00:16 2000



From: Margaret Brannigan :      brannign-at-asrr.arsusda.gov
Date: Wed, 20 Sep 2000 09:51:24 -0400
Subject: need source for LKB III repair in Washington DC

Contents Retrieved from Microscopy Listserver Archives
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Hi,

We have an old LKB III microtome that needs service. I called Leica
but have not heard back -- and maybe never will-- they said they
weren't sure they had anyone in the Washington DC area who could work
on them.

Does anyone know of someone in this area who might be able to help?
THere used to be a service engineer named Patricia Cappagrossi (I
think) but I can't find any numbers for her...

TIA

Margaret
--
Margaret Dienelt
Plant Pathologist

Electron Microscopy Lab
FNPRU, National Arboretum
ARS, USDA

B. 010A R. 238 BARC-W
10300 Baltimore Avenue
Beltsville, MD. 20705

Telephone: (301) 504-6097
Fax: (301) 504-5096



From daemon Wed Sep 20 09:23:00 2000



From: Tom Phillips :      PhillipsT-at-missouri.edu
Date: Wed, 20 Sep 2000 08:56:22 -0500
Subject: Re: Immuno TEM

Contents Retrieved from Microscopy Listserver Archives
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Paul Webster said "I don't think that any one of my collection of
100's of antibodies works only by LM. They all also work by EM. "

Wow, that is amazing. I have never heard anyone having any where
near that level of success. What type of epitopes are you looking
at? What tissues are they located in? I could understand it if you
were routinely using polyclonal antisera against carbohydrate
epitopes - my own work with mucin molecules has shown me that
carbohydrates are relatively easy to localize. How many of your
antisera were made against synthetic peptides?

Can you tell me what you do when you get an IgM monoclonal to make it
work at the EM level? I have never succeeded with one of them at the
EM level.

You don't give your success rate for antibodies at the LM level?
Have you succeeded with every antibody you have tried at the LM
level? I have tried numerous ones that work for westerns or
immunoppt that don't work at all at the light level (chemical
fixation, precipitant fixes, quick-freeze) on cryo, paraffin, resin
embedded protocols with or without a host of antigen retrival. Can
you give me some references to your publications that outline your EM
protocols for the "hard ones" or post your protocols on the list?

I routinely use 2% paraformaldehyde as a fix but vary this when I run
into failure. I routinely screen in LR White, LR Gold, and Lowicryl
K4M but in some (admittedly only a small percentage) have tried other
plastics and heroic procedures. I have used ultra-thin cryo sections
but, that it not really practical for some tissues (e.g., seed
tissues with their hard coat and softer insides). Likewise, not
every tissue is ammenable to nanogold permeabilization pre-embedding
approaches (e.g., seed tissue again) . What do you do when you have
tried 2% paraformaldehyde fixed tissues in LR White, LR Gold and
Lowicryl and it still doesn't work? I routinely use labeled
secondary antibodies to avoid the limitations with protein A. In
most of my cases, the antigen level should be sufficient to see if
the antibody worked.

How long does it take you to determine a successful protocol for each antibody?

You say "Keep preparation and labeling protocols the same for both
and you will enjoy much success." I am not sure how to translate a
positive result at the LM level with an acetone fixed whole mount of
tissue culture cells grown on coverslips to the EM level. Do you use
acetone or other precipitant fixes at the EM level? I have actually
tried that but, not surprisingly, the quality of tissue preservation
was not good enough for publication?

I know this is a lot of questions but your success rate is so much
higher than mine, I am quite interested in hearing the details of
your protocols.

Thanks, Tom




} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

--
Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)



From daemon Wed Sep 20 09:55:30 2000



From: Mark West :      mwest-at-ifcsun1.ifisiol.unam.mx
Date: Wed, 20 Sep 2000 10:13:03 -0600 (=?ISO-8859-1?Q?Hora_est=E1ndar_de_M=E9xico?=)
Subject: mu-metal

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Date sent: Tue, 19 Sep 2000 17:31:45 -0400
} From: "Spaulding, Robert F" {SpauldinRF-at-corning.com}


Hi,

A question about SEM interference. We have high electromagnetic field
levels (about 5 times the Jeol recommended limits) and our SEM isn't much
use above 10 000x mag (a Jeol 5410 low vacuum) - the focus is fuzzy and
astigmatism hard to correct. I've had water and air pipes removed from the
room, and we've been everywhere measuring fields but the fields and the
poor performance don't change. Changing the room is not an option (to the
people in charge of the institute!). Jeol recommended, ruling out a room
change, that we fit a mu-metal shield around the column. I have contacted
the company that makes the mu-metal and the thing would cost about
US$6000.00 and a hassle to ship to Mexico, so I don't want to go ahead and
find that we're in the same place with our performance.

Does anyone have experience with fitting a mu-metal shield to their
microscope (especially a scanning EM) and resolving (or not resolving!)
interference problems. I'd be very interested in hearing about your
experiences with this stuff.

Thanks,

Mark

PS - the serial sections are coming along nicely (not perfect,but heading
that way!), but I've still got a lot of ideas to try yet. Many thanks.






********************************************
Mark West,
Unidad de Microscopia Electronica,
(Electron Microscopy Unit)
Instituto de Fisiologia Celular,
Universidad Nacional Autonoma de Mexico,
04510 Mexico D.F.

tel (unidad/lab) *(525) 622 5610*
(casa/home) (525) 619 3020
Fax (525) 616 2282
********************************************





From daemon Wed Sep 20 11:52:55 2000



From: Rick Harris :      raharris-at-ucdavis.edu
Date: Wed, 20 Sep 2000 09:38:01 -0700
Subject: Re: Dye Sub printer speeds-a suggestion

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At 08:11 PM 9/19/00 -0700, Gary Gaugler wrote:
} How is this really different from what others are talking about? If the
} computer has to talk to a printer, or in your case, to a network,
} the path is still external. A pristine example is when the computer
} saves to a local disk. Then, the disk is physically carried to
} some other computer and accessed at that point. A network
} interface requires system resources just as any other interface
} does. Arguably, the network can take more resources than
} other interfaces. It is not benign.

I disagree. Very few system chores are as demanding as printing while
networking is inconsequential. Writing to a mapped drive on a 10/100 mbit
card is virtually transparent. If you have a local switch then it would be
entirely transparent. However, printing a 1 meg file, especially to a
postscript printer, can bring your system to it's knees (as it does in this
case). PS translation can easily increase a 1 meg file to many times it's
original size with a corresponding hit on performance while the information
is generated then spooled then sent to the printer. But that is not really
the point I was trying to make. My point is that it is well to consider
isolating your dedicated computers from everyday tasks such as printing and
allow them to manage the device for which they were designed.

Also, we do not use removable media to transfer images from devices and
prefer to save from the image acquisition device to a RAID 5
server. Removable media have too many failure points and generally require
more system resources. If you start the Task Manager on your NT
workstation and watch the performance hit while writing to a ZIP you can
see it is considerable. Writing to a HDD or a NIC are about equivalent and
much less than the ZIP. Printing locally takes a big chunk of CPU time and
sending the print to a print server takes the least of all. You should
also consider the printer setup. If you are sending 600 dpi files to a 300
dpi printer you are wasting system resources and gaining nothing in return
so it is well to check the printer setup on the two machines our original
letter writer was referring to. In my opinion, I think you should let the
server do the grunt work and let the dedicated computer manage the device.




Rick A. Harris, Director
Microscopy and Imaging Facility
Section of Molecular and Cellular Biology
1241 Life Sciences Addition
University of California
Davis, CA
530 752 2914
530 754 7536 fax
http://katie.ucdavis.edu
raharris-at-ucdavis.edu




From daemon Wed Sep 20 12:05:05 2000



From: Huggins, Bradley J :      HUGGINBJ-at-bp.com
Date: Wed, 20 Sep 2000 11:07:47 -0500
Subject: RE: ESEM beam problem

Contents Retrieved from Microscopy Listserver Archives
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Frank,
The other thing that can happen with a LaB6 is a rather severe accumulation
of "crud" on and around the Wehnelt aperture. With the very long lifetime
of the LaB6 crystal, you can accumulate significant deposit in this area. I
have seen similar instances where the crystal is still OK, but the
contaminants on the Wehnelt get very thick and begin to pull away from the
wall of the aperture and create distortions in the emission through the
Wehnelt assembly. In order avoid this I try to clean the Wehnelt cap
approximately every six months.
Good Luck,
Brad Huggins
BP Amoco, Naperville, IL

} ----------
} From: Frank Thomas[SMTP:thomasf-at-AGC.BIO.NS.CA]
} Sent: Tuesday, September 19, 2000 6:25 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: ESEM beam problem
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Listers-
}
} Our ESEM has developed a problem with its beam. I don't know if many
} other
} instruments have this, but there is a "source" mode button which , when
} hit, provides a sort of view "back up the column towards the gun". It's
} used for saturating the filament - when properly saturated, the beam
} should
} look like a nice round to oval bright spot. Our instrument has a LaB6
} source, which, because of the shape of the tip of the crystal, may produce
}
} four weaker "satellite" beams around the "main" beam - which is OK, you
} just want to be sure you're using the main beam.
} Just yesterday, and quite suddenly, this view changed somewhat - we
} no
} longer seem to have a nice oval spot, but instead a sort of cross shape,
} with one side smeared out into a separate, smaller beam. Signal has been
} dramatically reduced - I have to open up the condensor 'way more than I
} should, to get any kind of reasonable signal, and then the resolution is
} pretty degraded.
} I'm thinking the LaB6 has failed, perhaps with some small bit
} falling off
} one side. Previously, when one of these has died on us, it's pretty sudden
} and dramatic - suddenly no signal, period. Our gun vacuum is generally
} pretty good, but we have at least a thousand hours on this filament, so I
} suppose it may be on its last legs.
} Assuming I'm right in blaming it on the LaB6 - I can't think of any
} other
} reason for this problem - has anyone else had a filament fail in this way?
} I'd just like to hear of any other possible causes before I open up the
} gun. I suppose it's possible some little bit of crud found its way
} partially up the column and is causing severe astigmatism, but that sounds
} like a major disassembly effort, too.
}
} F.C. Thomas
} MicroAnalysis Facility
} Bedford Institute of Oceanography
} Dartmouth, Nova Scotia
} Canada
} Geological Survey of Canada (Atlantic)
} Bedford Institute of Oceanography
} Dartmouth, Nova Scotia
} Canada B2Y 4A2
}



From daemon Wed Sep 20 12:14:19 2000



From: White, Woody N :      Woody.N.White-at-mcdermott.com
Date: Wed, 20 Sep 2000 12:51:00 -0500
Subject: Re:mu-metal

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I am awaiting delivery of an "engineering kit" of muMetal from
Advance (about $100, online order). We have some EMI from the SEM
CRT which doesn't sound as bad as your situation, but does limit
resolution. Since it is not practical to move the monitor far
enough to mitigate the situation, I decided to try shielding.
I am not yet sure how I will fit in/around the chamber , but am
going to see if it will make a difference. Will let you know if
it successfully eliminates the "vibes".

Woody White
McDermott Technology, Inc.

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The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Hi,

A question about SEM interference. We have high electromagnetic field
levels (about 5 times the Jeol recommended limits) and our SEM isn't much
use above 10 000x mag
{SNIP}
********************************************
Mark West,
Unidad de Microscopia Electronica,
(Electron Microscopy Unit)
Instituto de Fisiologia Celular,
Universidad Nacional Autonoma de Mexico,
04510 Mexico D.F.

tel (unidad/lab) *(525) 622 5610*
(casa/home) (525) 619 3020
Fax (525) 616 2282
********************************************



From daemon Wed Sep 20 13:16:31 2000



From: Paul Webster :      pwebster-at-mailhouse.hei.org
Date: 20 Sep 00 11:07:48 -0700
Subject: Re: Immuno TEM

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From: Paul Webster :      pwebster-at-mailhouse.hei.org
Date: 20 Sep 00 11:07:48 -0700
Subject: Re: Immuno TEM

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Reply to: Re: Immuno TEM
Hi Tom,

Thanks for joining in. My posting was a good excuse to get people thinking about stuff they usually take for granted. I am surprized when people tell me about their lack of success with EM immunocytochemistry when often the problem can be solved by minor protocol modifications.

My antibodies really do all work by EM. I guess I have never really thought about why this is so, but one reason may be that I get to know the people who make them, find out as much as I can about what the antibodies label, and try to understand as much as I can about the biology of the system I work with. Some antibodies will only work on tissue culture cells that have been cultured for more than 15 days. Before this time, the antigen has not been produced in sufficent number and has not been inserted into it's functional site. I can go on with examples, but this really does not help you.

As for making IgM's work by EM, how do you store your antibodies? Do you make sure that the IgM's are not subject to freeze-thaw conditions? One round of this will break them up sufficiently to make them unuseable. Sometimes one freezing is enough if there is no cryoprotectant in the solution. Ask whoever made the antibodies if they had been frozen at least once.
The surprizing thing is that for LM, even low affinity antibodies can be detected. Imagine that only about 10% of the IgM is in good shape and all the rest is totally fragmented. This will have two effects. First, the antibody dilution will have to be increased for EM detection. Second, the fragments may still bind to antigens on your section, but because they are fragments, the secondary antibody will not recognise them (know your reagents). They have competed for the specific binding sites, have won, but cannot be detected by your visualization antibody. At the EM level, this is a disaster. For LM, where there will be many more antigens present, and where amplification techniques can be used, the signal will still be detected. Know how the IgM fragments, and know what the secondary antibody recognises, and you may be on the way to understanding why the antibody does not work.

Have you ever purchased streptavidin-gold to localize biotin at the EM level? This is another tricky reagent to work with because it always seems to detect biotin at the LM level using silver enhancer. At the EM level there is never a signal. Usually, the first question I ask is what blocking agent is being used. If serum is used, then the gold probe will bind to biotin-like molecules in the serum, thus eliminating the specific label. More importantly, it seems that this probe is very unstable with the gold seperating from the protein after only short storage times. The presence of free streptavidin in the solution is not sufficient to inhibit labeling at the LM level, but it does remove almost all the EM label.

ANother example I have concerns an antibody that worked by LM but not by EM. This was an antibody that was made to a protein synthesized by endothelial cells. The solution took me a week to work out. My colleagues could see immunofluorescence labeling if they followed the protocols in published papers using the same antibody, and could even repeat the published result. Whenever they tried the antibody by EM, there was literally no signal. Now usually, it is possible to see one or two gold aprticiles on a grid that has been exposed to gold probe.
After lots of thinking, and a few experiments, we found out that the specific antibody was being removed from our labeling solution. The blocking agent initially used to dilute the antibody was 10% serum. As the protein being studied was also a serum protein, the antibody was reacting to this molecule and we were getting a very good negative result (adsorption control). The authors of the published papers had never bothered to do obvious controls and were happy when they saw signal at the LM level. What were they looking at? It turns out that the cells they (and us) were using contained lots of autofluorescent organelles that were being interpreted as specific signal.

As for antibodies not working by LM. If I do not get a signal by LM, I give up the antibody as being no use to me. There is no way I would expect a signal by EM if I couldn't see it first by LM. I will usually screen antibodies first on the system I expect to use for EM labeling. Thin sections of Lowicryl-embedded material make really good LM preparations. It is possible to also test the EM immunoreagents by LM too. The silver enhancers are great for visualizing protein A-gold.

Limitations of protein A-gold, what are they? I think there are more disadvantages to using gold conjugated secondary antibodies. First, the resolution is not as good as with PAG. Second, the antibodies will deteriorate when stored. Antibody without gold will compete for binding sites and reduce the label. With PAG, one reagent, used by everyone in the lab will be less expensive and can also be easily monitored for quality. If everyone is using it successfully and one researcher has a problem, it is easy to identify where the problem is. If someone is using a secondary antibody that has not been used for 6 months, the troubleshooting procedure will be boring and time consuming.
My work is mostly with mammalian cells and pathogens and I do not have many antibodies to carbohydrates. Mostly they are to membrane proteins or to other cellular components. I think I have about 6 anti-peptide antibodies that work for me.

The mention of pathogens and antibodies brings up another point. Many antibodies are made using Freund's adjuvant, a common protocol for antibody preparation. This is a homogenate of ground-up bacterial proteins. If the study involves looking at bacteria it is inevitable that some background labeling on bacteria will be observed. If we know how the antibody was prepared, then we will not be surprized by this result. If we do not know the preparation protocols, then we will spend lots of time trying out different fixation and blocking solutions, eventually giving up saying that the antibody is no good.

I hope these posts help answer some of your questions. Contact me again if there are issues I didn't address.
Regards,

Paul.

Paul Webster, Ph.D
House Ear Institute
2100 West Third Street
Los Angeles, CA 90057
phone:213 273 8026
fax: 213 413 6739
e-mail: pwebster-at-hei.org
http://www.hei.org/htm/aemi.htm




From daemon Wed Sep 20 13:33:05 2000



From: Anthony Garratt-Reed :      tonygr-at-mit.edu
Date: Wed, 20 Sep 2000 14:18:40 -0400
Subject: Re: mu-metal

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Mark-

Are you sure that your problem is external EM interference? If it were
60Hz, then I would expect to see (at some scan rates and magnifications,
anyway) a "jagginess" in the images, rather than "fuzziness". Also, if it
is possible to lock the scan to the local power frequency, you could get
some other recognizable effects in the image. If it is not 60Hz, but a
much higher frequency, it may be getting into the 'scope circuitry and
affecting the scan drives or other parts of the optics, rather than the
magnetic field directly affecting the beam. A mumetal enclosure would not
help this. Even if it is 60Hz, it can get in via ground loops (often
accidentally set up) and would not be affected by mumetal.

Another totally different possibility is that you are blaming the wrong
thing, and in fact you have a problem elsewhere, but because of the known
high fields the service people have a "get out".

You say that your fields are 5 times the recommended, and that you cant use
the scope above 10,000. This would imply that with the recommended fields
the scope could be used up to 50,000. Is this realistic? (I don't know the
specs of the 5410). If not, the implication is that there is another fault.

These are just thoughts, I know, not solutions, but hope they help.

Tony G-R

}
} Hi,
}
} A question about SEM interference. We have high electromagnetic field
} levels (about 5 times the Jeol recommended limits) and our SEM isn't much
} use above 10 000x mag (a Jeol 5410 low vacuum) - the focus is fuzzy and
} astigmatism hard to correct. I've had water and air pipes removed from the
} room, and we've been everywhere measuring fields but the fields and the
} poor performance don't change. Changing the room is not an option (to the
} people in charge of the institute!). Jeol recommended, ruling out a room
} change, that we fit a mu-metal shield around the column. I have contacted
} the company that makes the mu-metal and the thing would cost about
} US$6000.00 and a hassle to ship to Mexico, so I don't want to go ahead and
} find that we're in the same place with our performance.
}
} Does anyone have experience with fitting a mu-metal shield to their
} microscope (especially a scanning EM) and resolving (or not resolving!)
} interference problems. I'd be very interested in hearing about your
} experiences with this stuff.
}
} Thanks,


** Anthony J. Garratt-Reed
** MIT Room # 13-1027
** 77 Massachusetts Avenue
** Cambridge, MA 02139-4307
** USA
**
** Phone: (+) 1-617-253-4622
** Fax: (+) 1-617-258-6478
**





From daemon Wed Sep 20 13:47:52 2000



From: David Su :      davids-at-accurel.com
Date: Wed, 20 Sep 2000 11:27:27 +0100
Subject: Position Open: Dual Beam FIB Engineer

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Dual Beam FIB Engineer

Accurel Systems International Corp. has an immediate opening for a Dual Beam
FIB Engineer.

Responsibilities include operating an FEI 820 and/or 835 Dual Beam FIB
system to prepare SEM and TEM cross-sections for external customers on a
commercial basis.

Qualified candidates must possess a B.S. or M.S. degree in the physical
sciences or engineering or equivalent expertise. Although prior experience
with FIB is not required, it is advantageous. Familiarity with IC
processing and magnetic materials would also be advantageous.

Accurel Systems is an independent commercial laboratory located in the heart
of Silicon Valley, 40 miles south of San Francisco. The laboratory is also
well equipped for SIMS, Auger, ESCA, TEM, SEM AFM, FIB, XRD, TXRF, Failure Analysis Services etc.

For confidential consideration, please send your resume to:

Amy Hunt, Accurel Systems International Corp., 785 Lucerne Drive, Sunnyvale,
CA 94085.
Email: amyh-at-accurel.com
Fax: (408)737-1608



David Su, Ph.D.
Director of FIB/TEM Technology Development
Accurel Systems International Corp.
785 Lucerne Drive
Sunnyvale, CA 94086
Tel.: (408)737-3892 x102
FAX :(408)737-3916
email: davids-at-accurel.com or davidsu-at-aol.com




From daemon Wed Sep 20 13:50:29 2000



From: Philip Oshel :      peoshel-at-facstaff.wisc.edu
Date: Wed, 20 Sep 2000 13:41:52 -0500
Subject: Old Edward vacuum evaporator parts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,

Does anyone have an old Edwards E306A (manual) vacuum evaporator
mouldering in their lab? I need either a rototilt 3 electrical
feedthrough or a blank plug for a size "B" hole. The same parts for
the *A*306 (automatic) won't work, as Edwards changed the hole size
when they changed models.

Thanks!

Phil
--
}}}}}}}}}}}}}}}}}{{{{{{{{{{{{{{{{{
Philip Oshel
AMFSC and BBPIC
Dept. of Animal Health and Biomedical Sciences
University of Wisconsin
1656 Linden Drive
Madison, WI 53706-1581
voice: (608) 263-4162
fax: (608) 262-7420 (dept. fax)



From daemon Wed Sep 20 14:17:43 2000



From: Greg Erdos :      gwe-at-biotech.ufl.edu
Date: Wed, 20 Sep 2000 15:04:23 -0400
Subject: Immuno TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Mark,
Are you sure you have a em field problem? They usually show up as a
large(assuming the frequency is low, 50-60hz) sawtooth on the displayed
image. This sawtooth will change in appearance with different sem scan
rates, i.e., at TV rate, the images appears to "shake" back & forth, and at
the slowest visual rate, will exhibit a very well defined sawtooth. If you
do have a photo CRT(probably not), the photo scan is usually synchronized to
the AC line frequency, hence negating the effect of any EM interference,
again, assuming your "external fields" are the same frequency. Did you shut
down the SEM completely, and disconnect it from its power source when making
your field checks? This is very important.
If the symptoms are a fuzzy image and high astigmatism, I would check my
SEM parameters to ensure that the controls are properly set for high
resolution - WD, spot size, aperture size, tilt, etc.. If these are okay,
look for dirt somewhere in the column, column mis-alignment, poor vacuum
levels. Also make sure that the beam crossover pole pieces are inserted
correctly and that the column liner tube apertures are in the correct spot.
Has this problem always been there since the instrument was initially
installed, or did it develop afterwards. I really don't think mu-metal will
fix your problem. Good Luck!

Gary M. Easton
Scanners Corporation
Third Party SEM Service

----- Original Message -----
} From: "Mark West" {mwest-at-ifcsun1.ifisiol.unam.mx}
To: {microscopy-at-sparc5.microscopy.com}
Sent: Wednesday, September 20, 2000 12:13 PM


We have a very low success rate as well, but I am convinced that it is
because we are a fee for service lab and the PI's do not want to take the
time or spend the money on the numerous trials that might be required to
get acceptable results. I think, that if given the opportunity, we could
have a much better success rate. But we are treated as a commodity and not
a research endeavor.
Greg Erdos
Gregory W. Erdos, Ph.D.
Assistant Director, Biotechnology Program
PO Box 110580
University of Florida
Gainesville, FL 32611

Ph. 352-392-1295 Fax: 352-846-0251



From daemon Wed Sep 20 15:36:32 2000



From: Gary Lovell :      gllovel-at-ppco.com
Date: Wed, 20 Sep 2000 15:22:52 -0500
Subject: JEOL vs CAMECA

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I would be interested in heaing from anyone who has done a market survey on
JEOL 8200 versus CAMECA SX100 microprobes. Which ( in the responders
opinion ) has the better system for geological quantitative WDS analysis,
or performs better in the SEM mode. Please give the reasons behind your
preference, if at all possible. PLEASE, NO VENDOR RESPONSE.

Gary L. Lovell
Phillips Petroleum Company
245a GB
Bartlesville, Ok. 74004
Phone: (918)661-9691
Fax : (918)662-2047
Email: gllovel-at-ppco.com




From daemon Wed Sep 20 15:37:05 2000



From: Ronald LHerault :      lherault-at-bu.edu
Date: Wed, 20 Sep 2000 16:27:02 -0400 (EDT)
Subject: Citrated Saline

Contents Retrieved from Microscopy Listserver Archives
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One of our students needs a receipe for citrated saline and cannot find it
in the very limited references we have available here (a materials lab
where one group is doing some tissue culture). She has seen it mentioned
in articles but it must be so basic (or maybe acidic) that no one tells
how it is made. I hope someone can enlighten us.

Thanks.

Ron
lherault-at-bu.edu





From daemon Wed Sep 20 16:25:47 2000



From: Glen MacDonald :      glenmac-at-u.washington.edu
Date: Wed, 20 Sep 2000 14:19:23 +0100
Subject: Microscopy and Imaging workshop

Contents Retrieved from Microscopy Listserver Archives
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Microscopy and Digital Imaging: Advances and Applications

University of Washington
Seattle, WA

October 5, 2000
Room CD150, CHDD Early Education Unit
8:30 AM to 4:30 PM

A day of presentations, demonstrations and refreshments. The
first in a series of opportunities to learn and share knowledge about
all aspects of microscopy and digital imaging for biomedical research.


Confocal Microscope Demonstrations October 4-6th:
Bio-Rad - Radiance laser scanning confocal
Solamere Technologies - Spinning disk confocal



Sponsored by:
The UW Center on Human Development and Disability
The W.M. Keck Center for Advanced Studies in Neural Signaling
The Virginia Merrill Bloedel Hearing Research Center
Supported by the Bio-Rad Corporation.


Please RSVP to Glen MacDonald, glenmac-at-u.washington.edu,
616-4156, to register for attendance to the workshop, confocal
demonstrations or to meet with Dr. Johnson and Dr. Danilchik.
Lunch will be provided for all who pre-register by October 2nd.



Schedule:
8:30 Welcome
8:45 Glen MacDonald, UW CHDD/VMBHRC - “What is in a Digital Image, What
Happens When You Enhance It?”
9:45 Coffee and snacks
10:00 Iain Johnson, Molecular Probes - “Introduction to Fluorescence and
Fluorescent Probes”
11:00 Mike Danilchik, University of Oregon Health Sciences Center -
“Using Confocal Microscopy to Study Early Events in Xenopus laevi Development”
12:00 Lunch
1:00 Peter Rabinovitch, UW Dept. Pathology - “ Telomere Length Measurements
in Tissue Sections by Quantitative FISH Confocal Microscopy”
2:00 John Jordan, Bio-Rad - “Recent Developments in Confocal Microscopy
at Bio-Rad”
2:40 George Peeters, Solamere Technology - “Introducing the Spinning
Disk Confocal Microscope and its Applications ”
3:00 Coffee and snacks
3:15 Iain Johnson - “A Demonstration of Web-based Resources for
Fluorescent Microscopy, Where Does the Molecular Probes’ Technical
Support Staff Get Their Information?”





--

Glen MacDonald
Virginia Merrill Bloedel Hearing Research Center
Box 357923
University of Washington
Seattle, WA 98195-7923
glenmac-at-u.washington.edu
(206) 616-4156
(206) 616-1828 fax
************************************************************************
C:} The box said "Requires Windows95 or better". So I bought a Macintosh.
************************************************************************



From daemon Wed Sep 20 16:35:14 2000



From: L. D. Marks :      ldm-at-risc4.numis.nwu.edu
Date: Wed, 20 Sep 2000 16:39:39 -0500 (CDT)
Subject: Re: mu-metal

Contents Retrieved from Microscopy Listserver Archives
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We have not done this for a SEM, but have mu-metal
around our TEM, and it works very well. Before you
go to the expense, you may want to try some soft
iron as an experimental shield. It is not the best
(and certainly not pretty) but it works and will
tell you if the problem really is fields and not
something else.

++++++++++++++++++++++++++++++++++++++++++++++++
Laurence Marks
Department of Materials Science and Engineering
Northwestern University
Tel: (847) 491-3996 Fax: (847) 491-7820
mailto:ldm-at-risc4.numis.nwu.edu
http://www.numis.nwu.edu
++++++++++++++++++++++++++++++++++++++++++++++++




From daemon Wed Sep 20 16:42:23 2000



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Thu, 21 Sep 2000 10:07:19 GMT+1200
Subject: wds spectros on 840

Contents Retrieved from Microscopy Listserver Archives
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Are you running the scope in high vacuum mode when you have these problems?
What are the operating conditions and sample characteristics when you have
this trouble?

I have trouble on our Hitachi 2460N getting decent pictures above 5kx using
40Pa of He. I also re-proved the point that gold coatings are beneficial. I
was looking at some silicon samples in our JEOL 840A at 10kx. I could get
images without charging, but the quality was poor. The pictures looked much
better with gold since the secondary signal was stronger and generally
coming from an area closer to the beam.

At 10:13 AM 9/20/2000 -0600, you wrote:
} Hi,
}
} A question about SEM interference. We have high electromagnetic field
} levels (about 5 times the Jeol recommended limits) and our SEM isn't much
} use above 10 000x mag (a Jeol 5410 low vacuum) - the focus is fuzzy and
} astigmatism hard to correct. I've had water and air pipes removed from the
} room, and we've been everywhere measuring fields but the fields and the
} poor performance don't change. Changing the room is not an option (to the
} people in charge of the institute!). Jeol recommended, ruling out a room
} change, that we fit a mu-metal shield around the column. I have contacted
} the company that makes the mu-metal and the thing would cost about
} US$6000.00 and a hassle to ship to Mexico, so I don't want to go ahead and
} find that we're in the same place with our performance.
}
} Does anyone have experience with fitting a mu-metal shield to their
} microscope (especially a scanning EM) and resolving (or not resolving!)
} interference problems. I'd be very interested in hearing about your
} experiences with this stuff.
}
} Thanks,
}
} Mark
}
} PS - the serial sections are coming along nicely (not perfect,but heading
} that way!), but I've still got a lot of ideas to try yet. Many thanks.

----------------------
Warren E. Straszheim
Materials Analysis and Research Lab
Iowa State University
23 Town Engineering
Ames IA, 50011-3232

Ph: 515-294-8187
FAX: 515-294-4563

E-Mail: wesaia-at-iastate.edu
Web: www.marl.iastate.edu

Scanning electron microscopy, x-ray analysis, and image analysis of materials
Computer applications and networking


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Hi

Any one still awake who can tell me which ports to mount 3 x wds
spectros on an 840 which has the OM?

The three spectros are

STE/RAP
PET/LIF
PET/LIF

The three ports on the head of the OM aren't the same, which leads me
to suspect that the positioning may not be arbitrary.

Please express your answer numbering the ports clockwise from the
operator, bird's-eye view, 1, 2, 3.

rtch


Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand



From daemon Wed Sep 20 20:05:01 2000



From: Karen Pawlowski :      karen.pawlowski-at-worldnet.att.net
Date: Wed, 20 Sep 2000 19:51:40 -0500
Subject: EM facilities in the Dallas/Ft. Worth area

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All,

Does anyone out there know of a SEM/TEM facility in the DFW area that is
willing
to rent time on their machine? .

I'm posting this for a colleague who has been using both SEM and TEM
facilities
on a pay for use basis for forever. Unfortunately those facilities are
under new
management who are reluctant to give outside users access to their
equipment.
So he is looking for alternatives. Thanks in advance for your input.

Karen Pawlowski, Ph.D.







From daemon Wed Sep 20 20:47:48 2000



From: laniven-at-julian.uwo.ca ()
Date: Wed, 20 Sep 2000 20:26:39 -0500
Subject: What is GFP?

Contents Retrieved from Microscopy Listserver Archives
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Email: laniven-at-julian.uwo.ca
Name: Leigh Ann
School: UWO
Question: What is GFP? How do you convert a microscope for birefringence
and what type of microscopy is this?

---------------------------------------------------------------------------





From daemon Wed Sep 20 20:47:48 2000



From: Gary M. Easton :      gary.easton-at-scannerscorp.com
Date: Wed, 20 Sep 2000 20:15:50 -0500
Subject: Carbon tracking

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Listers, Anyone out there know of a good(hopefully easy!) way to
remove carbon tracking deposits on a ceramic electon gun? Thanks in
advance. Gary M. Easton Scanners Corporation





From daemon Wed Sep 20 21:04:08 2000



From: ard-at-ansto.gov.au (Arthur Day)
Date: Thu, 21 Sep 2000 14:50:18 +1000
Subject: Re: Dye Sub printer speeds- followup

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Date sent: Wed, 20 Sep 2000 10:13:03 -0600 (Hora estándar de México)
} From: Mark West {mwest-at-ifcsun1.ifisiol.unam.mx}
To: microscopy-at-sparc5.microscopy.com


} I found I can print via the parallel port to the printer just fine from a
} second computer (Dell 450) in the lab, using freshly reinstalled postscript
} drivers. A 1.2MB file prints in about 1 minute and the transfer to the
} printer (Progress in the Systray Printer icon box) goes in a few seconds.
} In turn the same file, same cable, same software, both win 95, but
} different computer (Compaq 333 attached to my Hitachi SEM) takes 4.5 minutes
} to get over the printer, before printing starts, transferring in blocks of
} about 60kB, 1 every 8-10 seconds. I am not logged onto the network, and
} have disabled my Norton anti-virus software.
}
} This experiment rules out a lot of issues and now I'm focusing on the setup
} of my SEM computer to see why the Progress goes so slowly on it while it
} goes so much faster on the Dell. If anyone has ideas about THIS issue I'll
} gladly try them out. Maybe Hitachi's computer guy has some ideas.

Basically, you are using the wrong kind of OS and hardware for trying to do
two things at once. "Go unix :-("

It seems that the key feature here is that the computer is also trying to
control the SEM at the same time. Presumambly there is a resources
bottlenck because the computer is desperately trying to do too many things
in too many little chunks too often? If you've checked out the printing
side and that's OK then maybe take a look at how the computer is talking to
the microscope. At what rate does the computer normally try to "converse"
with the microscope for example. If you used to be able to run the
microscope and simultaneously print in reasonable time when the system was
first installed, then perhaps this microscope polling rate (or whatever)
has been subsequently increased excessively. Too fast a rate could cause an
excessive slowdown when you try to get the computer to do something else
intensive such as printing. Will your microscope software allow you to find
out how often the computer talks to the microscope and allow you to change
it? You might only need to slow down the rate of conversation with the
microscope slightly to get a big improvement in both operations. Some
experimentation might allow you to find an acceptable balance between
printing time and microscope responsiveness.

Just a thought.

}
} Richard Shalvoy
} ---------------------
} original note
}
} I have a Kodak 8670 dye sub printer hooked up to my SEM, but it seems to me
} that it takes a long time to get it to print and also while it is in the
} printing process my computer (and hence my SEM) is locked up. Here are the
} details.
}
} I have the printer (1 year old) hooked to a PC with a 333 Pentium II
} processor (128 MB RAM) using a parallel cable. I do get the same
} performance when printing from other computers so I doubt that it is
} anything peculiar to this one computer.
}
} Now when I call for a printout it takes 5 minutes to get the 1MB file size
} print done, and almost all of the time is spent sending data to the printer
} - the actual printing takes a short time (30 sec). I have discussed this
} with Kodak Tech Support and they claim my performance is not unusual.
} HOWEVER, I also experienced much quicker printing times (I'm sure - I have
} notes, but it was a while ago) when I was first using the printer (1 minute
} / 1 MB of file size in the image) and this is confusing me.
}
} My question is: how fast should this sort of printer print when using the
} parallel cable to feed data to it? Is 5 minutes / 1MB unusually slow? I
} get frustrated particularly as my computer is locked up while the data is
} being sent out and I cannot continue imaging until the data dump is done. I
} have the Win 95 spooler running of course and I don't have this problem with
} my HP 970 inkjet which I use normally.
}
} I will welcome any good ideas or cold realities that apply.
}
} Richard Shalvoy
} Arch Chemicals
} 350 Knotter Drive
} Cheshire, CT 06410
} 203-271-4394

Arthur Day, Electron Microscope Unit Phone: 61-2-9717-3457
Ansto Materials Division Fax: 61-2-9543-7179
PMB 1, Menai (Sydney), NSW, 2234 Email: ard-at-ansto.gov.au
Australia www: http://www.ansto.gov.au/





From daemon Thu Sep 21 00:14:14 2000



From: bobrob-at-uswest.net
Date: Wed, 20 Sep 2000 22:10:41 -0700
Subject: SEM: mu-metal/Stray Field Problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Mark,

Before heading down an expensive path with the mu-metal, check out
a few basic things first. The power source and step-down transformer
would be easy to verify if they are correct.

Make sure the power is wired with a ground that runs all the way back
to the distribution panel and is not grounded to the local disconnect
box
which would be a "conduit" ground. This type of ground is a good source

for noise to the microscope. Also, have a look at the step-down
transformer.
This could be incorrectly wired too.

I would imagine with a 5410 you could roll it down the hall, plug it in
and
see if it changes anything.

Bob Roberts
EM Lab Services, Inc.
2409 S. Rural Rd Suite C
Tempe, Arizona 85282
(480) 967-3946




From daemon Thu Sep 21 01:28:27 2000



From: Garry Rosewarne :      g.rosewarne-at-pi.csiro.au
Date: Thu, 21 Sep 2000 17:03:37 +1000
Subject: LM doncaster dish

Contents Retrieved from Microscopy Listserver Archives
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I am after a supplier (preferably in Australia) for some doncaster dishes.
These are glass petri dishes / watchglass dishes containing raised,
concentric circles.
Thanks
Garry


Dr. Garry Rosewarne
Plant Industry
CSIRO, Black Mountain

GPO Box 1600
Canberra, ACT 2601

Phone: (02) 6246 4947
Fax: (02) 6246 5000
email: garry.rosewarne-at-pi.csiro.au



From daemon Thu Sep 21 02:04:09 2000



From: Nelson Conti :      NelsonC51-at-excite.com
Date: Wed, 20 Sep 2000 23:49:34 -0700 (PDT)
Subject: Re: What is GFP?

Contents Retrieved from Microscopy Listserver Archives
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On Wed, 20 Sep 2000 20:26:39 -0500, laniven-at-julian.uwo.ca wrote:

| ------------------------------------------------------------------------
| The Microscopy ListServer -- Sponsor: The Microscopy Society of America
| To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
| On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
| -----------------------------------------------------------------------.

|
| Email: laniven-at-julian.uwo.ca
| Name: Leigh Ann
| School: UWO
| Question: What is GFP? How do you convert a microscope for birefringence
| and what type of microscopy is this?
|


Hi Leigh Ann -
I can answer the first part of your question (to some extent), but I'm not
very sure about the second part.
GFP = Green Fluorescent Protein ; it's a protein that naturally
fluoresces, and I believe that it was first discovered as a natural product
from some marine animal (name ?). What I know of GFP is that it's gaining
usage as a fluorescent marker. I believe it's been used as a marker for
determining the location of X compound in a cell when viewed by fluorescent
microscopy, but I'm sure that other usages have been cited in other research
papers.
As for the second part ... someone in this list may correct me, but I
believe that birefringence is correlated with polarized light. You'll
definitely need to consult with someone else in this list to get more
information.
Good luck getting more answers.
Nelson Conti





_______________________________________________________
Say Bye to Slow Internet!
http://www.home.com/xinbox/signup.html




From daemon Thu Sep 21 02:48:19 2000



From: Ron Doole :      ron.doole-at-materials.oxford.ac.uk
Date: Thu, 21 Sep 2000 08:38:54 +0100 (GMT Daylight Time)
Subject: Re: Carbon tracking

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Gary,

I assume that the tracks are on a ceramic insulator which
is not glazed, I am not sure what might happen if a glazed
surface is abraded.

Alumina powder made up to a paste in Alcohol is good for
polishing out tracks on high voltage insulators (guns and
accelerators). If it is really bad then grit blasting with
Alumina powder may be needed. Make sure you don't damage
seals or other surfaces and that you wash thoroughly to
remove all traces of the powder. (That's wash the gun of
course, you should not get it all over yourself.) The
insulator should be gently baked out in a (clean) vacuum
oven to remove the final traces of solvent, but you may get
away with baking out gently in air and pumping out for a
long time before applying the voltage.

Beware of the limitations imposed by other materials used
in the constuction of the component, epoxy resins etc.

Good luck,
Ron

On Wed, 20 Sep 2000 20:15:50 -0500 "Gary M. Easton"
{gary.easton-at-scannerscorp.com} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Listers, Anyone out there know of a good(hopefully easy!) way to
} remove carbon tracking deposits on a ceramic electon gun? Thanks in
} advance. Gary M. Easton Scanners Corporation
}
}
}

----------------------
Mr. R.C. Doole
Department of Materials,
University of Oxford.
Parks Road, Oxford. OX1 3PH. UK.
Phone +44 (0) 1865 273701
Fax +44 (0) 1865 283333
ron.doole-at-materials.ox.ac.uk




From daemon Thu Sep 21 07:10:49 2000



From: Per =?iso-8859-1?Q?H=F6rstedt?= :      per.horstedt-at-pathol.umu.se
Date: Wed, 20 Sep 2000 13:53:07 +0200
Subject: Re:Re: SEM need help on endothelial monolayers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Rosemary,

the dryers we're using are a couple of Polaron E-3000 CPD's, and they are
working perfectly. The main advantage is that you manually control inlet-
outlet- and evacuation valves, which means you can totally control the
substitution of dehydration agent when the chamber is closed. Temperature
is controlled with ordinary tap water, controlled by an ordinary
thermostat-mixer. They also have a large viewing window that makes it very
easy to visualize everything that goes on inside the chamber, i.e. for
example the level of liquid CO2 which is very important when flushing the
chamber.
The chamber has a large volume that can contain many and/or large samples.
I've had a workshop making some sets of specimen carriers for different
types of specimen, so for example when working with biopsies, we can
prepare 24 at the same time.
We've used them for many years now and I find them highly recommendable.
Yours sincerely

Per Hörstedt
Department of Medical Biosciences
Pathology
Unit for Electron Microscopy
University of Umeå
S-90187 Umeå
Sweden

phone int-46-90-7851541
fax int-46-90-7851215




From daemon Thu Sep 21 07:15:29 2000



From: Earl Weltmer :      earlw-at-pacbell.net
Date: Thu, 21 Sep 2000 05:20:10 -0700
Subject: Re: Carbon tracking

Contents Retrieved from Microscopy Listserver Archives
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Building test shields from 0.30 cent per pound iron is a good test for how
well MU metal will work.. If you have an Ag Engineering department you
might be able to borrow it from them at no cost except on favor to be
announced at a later date. .30 cent iron my be less expensive.

Gordon
Gordon Couger gcouger-at-couger.com
Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00
----- Original Message -----
} From: {"bobrob-at-uswest.net"-at-sparc5.microscopy.com}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Thursday, September 21, 2000 12:10 AM


HCL works great as long as you protect the other (metal) elements.

Earl

"Gary M. Easton" wrote:

} ------------------------------------------------------------------------
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} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
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}
} Listers, Anyone out there know of a good(hopefully easy!) way to
} remove carbon tracking deposits on a ceramic electon gun? Thanks in
} advance. Gary M. Easton Scanners Corporation




From daemon Thu Sep 21 07:42:40 2000



From: werner.wittke-at-leica-microsystems.com
Date: Thu, 21 Sep 2000 07:58:23 -0500
Subject: GFP

Contents Retrieved from Microscopy Listserver Archives
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-----Original Message-----
} From: Granville
Sent: Thursday, September 21, 2000 1:51 PM
To: Luc




Hi Ann,
GFP is the Green Fluorescence Protein. Jellyfish Aequorea produces
bioluminescence from two proteins, aequorin, which is a Ca++ binding
protein that produces blue light. GFP emits green light by absorption of
the aequorin. GFP was discovered in1962 by O. Shimomura. In 1992 D.Prasher
and colleagues sequenced it. Now there are variants of GFP. GFP is a 238
amino acid protein. Absorption at 395 nm and 475 nm, emission at 508 nm. It
can expressed in a cell/tissue /animal with along another protein of
interest. It is, therefore, a reporter or label. It can be used to report
gene expression or gene transfer, for a highly specific label for protein
localization, quantification or dynamics.
greetings
Werner





From daemon Thu Sep 21 08:15:44 2000



From: Sadhukhan, Pat :      SadhukhanPat-at-BFUSA.com
Date: Thu, 21 Sep 2000 08:01:42 -0500
Subject: LKB III Repair

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Margaret,
You may call Norman J. Woodside, an ex-LKB employee, and he may help you.
His address and phone and fax numbers:
NJW Supplies
7 Tanglewood Rd.
Catonsville, MD 21228
Phone: 410-744-9574; Fax 410-744-1580
Thanks!
Pat





From daemon Thu Sep 21 08:28:29 2000



From: Robert H. Olley :      r.h.olley-at-reading.ac.uk
Date: Thu, 21 Sep 2000 14:14:13 +0100 (GMT Daylight Time)
Subject: Re: What is GFP?

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Leigh Ann,

Birefringence is observed under a light microscope by putting a polarizer
before the specimen (and generally before the condenser) and a similar
device called the analyser between the specimen and the eyepiece: the
analyser is usually set at 90^ to the polarizer. If the crystal (or even
a piece of stretched plastic or a blob of liquid crystal) has different
refractive indices in different directions, the polarized light passing
through the specimen gets divided into two out-of-phase components which
are recombined in the analyzer to give pretty interference colours, from
which one can also make quantitative deductions. The people that use this
technique most are geologists and mineralogists looking at thin polished
rock sections, but we plastics people also look at polymers in this way.
I'm not sure if it's used much in bio/med applications.

A good book to explain the physics and applications of the technique is:

Wood, Elizabeth Armstrong
Crystals and light.

while there are many books which concentrate more on the setup of the
microscope. Here's a selection out of our library catalogue:

Hartshorne, N.H.
Crystals and the polarising microscope.

Polarized light microscopy by Walter C. McCrone, Lucy B.McCrone
and John Gustav Delly

The polarizing microscope by A. F. Hallimond
Hallimond A.F.

+------------------------------------------------------------------------+
| Robert H.Olley Phone: |
| J.J.Thomson Physical Laboratory {direct line +44 (0) 118 9318572 |
| University of Reading {University internal extension 7867 |
| Whiteknights Fax +44 (0) 118 9750203 |
| Reading RG6 6AF Email: R.H.Olley-at-reading.ac.uk |
| England URL: http://www.reading.ac.uk/~spsolley |
+------------------------------------------------------------------------+

} Email: laniven-at-julian.uwo.ca
} Name: Leigh Ann
} School: UWO
} Question: What is GFP? How do you convert a microscope for birefringence
} and what type of microscopy is this?




From daemon Thu Sep 21 08:38:12 2000



From: Paul Webster :      pwebster-at-mailhouse.hei.org
Date: Thu, 21 Sep 2000 08:24:07 -0500
Subject: Re: Immuno TEM

Contents Retrieved from Microscopy Listserver Archives
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Tamara Howard writes:
} Maybe the morphology would suffer a little, but as this isn't a
} problem for LM, why should this trouble us at the EM level? We only
} require the morphology to enable us to identify labeled structures.
} I depend on decent morphology to tell me that I haven't cause
redistribution of the antigen/mRNA/gene at some time during the
processing. If the sample looks like mush but has nice label, how am I to
know that the label is where it was in vivo? I realize this may be
extreme, to assume that everything is going to move if not properly fixed,
but I think that it critical to keep this possibility in mind when
localizing anything, at any level of detection.


I seem to have caught someone's attention with my very controvertial post.
First let me address Tamara's question, which also covers part of an issue
brought up by Tom Phillips.
You take exception that I should advocate the use of poorly fixed material
for EM localization. Yet, at the LM level this same poor fixation is
considered a routine part of the protocol. How many people who perform LM
immunolocalizations routinely freeze the tissue without aldehyde fixation
or cryoprotection and then fix the sections with acetone or methanol,
air-drying them before or after this treatment? Who would conside that
they are working with a) badly fixed material?, b) material where the
target antigens have either been removed or relocated? My guess is that
very few people have even given this a thought. [If you think that
performing LM immunochtochemistry using aldehyde fixed material followed by
detergent permiabilization (without drying the sample) may I direct you to
Hannah, Weiss & Huttner 1998 Differential extraction of proteins from
paraformaldehyde-fixed cells: Lessons from synaptophysin and other
membrane proteins Methods (a companion to Meth. Enzymol.) vol16 pp170-181)].

These issues about morphology are never taken into consideration at the LM
level. Why then should we decide it is important to have good morphology
at the EM level? One reason is that we are attempting perfection and feel
that good morphology is aesthetically pleasing. More importantly we need
to have sufficient contrast information within the sections to obtain a
colocalization with recognizable subcellular structures (and hopefully see
the antigen location at sites where it exists in vivo).

If we are able to perform multiple labeling experiments, where we can use
one antibody to identify specific organelles, then absolute morphology is
not important. We can disrupt our cells so that they become bags of
loosely packed organelles and our antibodies have total accessibility to
antigens. We can even partially purify these organelles and work with them
as a pellet or adsorbed onto specimen grids. We will loose spatial
information if we do this, but we will get a signal.

Similarly, if the only way we can get a signal by LM is by air drying and
acetone fixing, then use the same protocol for EM. The protocol will be
easy (pre-embedding label) but the morphology will be awful. Remember,
this is what light microscopists accept as good morphology! Once the label
system has been established to work by EM (ie all the reagents work to give
a result), then it will be possible to start modifying the protocol to
improve morphology. Perhaps fixing the sample in 2% formaldehyde for 30
seconds, followed by a short homogenation (improve membrane preservation
and control cellular disruption) is the answer. Maybe a fixation in 5%
glutaraldehyde in a low osmolality buffer, so that the cells swell, is the
answer.
The exact recipe will depend on what is known about the antigen. If it is
a membrane protein, is the epitope deeply embedded inside the membrane? If
so, will this require membrane disruption (freeze-thaw, detergent?) or will
it be sufficient to just remove the cytoplasm? Each system has to be
approached as a completely new project and each will have an answer.
Knowing as much as we can about the antigen is our first step to success,
allowing ourselves to work with samples that do not have "text-book"
quality morphology is the next step. Being totally open about how we are
willing to prepare samples and look at our results, completes the process.
My protocols will not help many people with their own particular
immunolabeling problems but my experience might. So will the experience of
all the other researchers who have faced similar problems. Many different
immunolabeling protocols are published but, not surprizingly, are not
broadcast in technical journals. The best protocols are hidden in
scientific papers where the approach was unique and led to an interesting
discovery about a biological system. As I said, the methods are unique to
each system. There is little point in publishing lists for someone to
follow without thinking.

We have to face the fact that whatever method we use to prepare our sample,
each will have limitations. Our role is to try to make these limitations
work in our favor. Knowing what the limitations are for each preparation
protocol makes our job a little easier.

Do you want my replies to Tom Phillips posted here, or should I reply to
him off-line? I am sure there are many people already bored by this
discussion.

Regards,

Paul Webster.

Paul Webster, Ph.D
House Ear Institute
2100 West Third Street
Los Angeles, CA 90057
phone:213 273 8026
fax: 213 413 6739
e-mail: pwebster-at-hei.org
http://www.hei.org/htm/aemi.htm






From daemon Thu Sep 21 09:20:22 2000



From: Anne C Lewin :      anne.lewin-at-bms.com
Date: Thu, 21 Sep 2000 10:09:13 -0400
Subject: subscribe

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subscribe




From daemon Thu Sep 21 09:46:03 2000



From: Leona Cohen-Gould :      lcgould-at-mail.med.cornell.edu
Date: Thu, 21 Sep 2000 10:37:02 -0400
Subject: Re: Immuno TEM

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At 3:04 PM -0400 9/20/00, Greg Erdos wrote:
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Lee

Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175





From daemon Thu Sep 21 09:52:21 2000



From: Leona Cohen-Gould :      lcgould-at-mail.med.cornell.edu
Date: Thu, 21 Sep 2000 10:43:47 -0400
Subject: Re: What is GFP?

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At 8:26 PM -0500 9/20/00, laniven-at-julian.uwo.ca () wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Dear Leigh
GFP stand for green fluorescent protein. It is a naturally fluoescent
protein, originall isolated from a jelly fish and nowavailable as a
construct that you can insert into your protein of choice using routine
molecular biology techniques. It obviates the need for immuno-labelling.
In order to see it, you need a UV light source that emits at/around 488nm
and the appropriate fluorescence filter sets. The ones designed for FITC
work just fine. YOu'd need to add a fair number of components to your
'scope...if you can convert it. Your local microscope sales/service person
will be able to gie you the details.

Lee

Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175





From daemon Thu Sep 21 10:31:17 2000



From: anderron-at-us.ibm.com
Date: Thu, 21 Sep 2000 11:21:01 -0400
Subject: JEOL vs CAMECA

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I'm sorry, but I think questions like yours aren't fair. You may get a
number of very public gripes from people who may have a good point OR are
getting bad results because of their poor skills. Respectable vendors are
defenceless against these things because if they rebutted all complaints it
could look like they were petty or argumentative or had something to hide.
Consider the Golden Rule.

I can remember seeing a respected vendor raked over the coals by a TEM
operator who claimed that the TEM in question was unstable. It turns out
that he liked bright screens (easier to focus) and recorded all of his
pictures of biological tissue specimens with the largest beam size and the
beam at crossover.

It's best that you conduct your own comparisons, using your own
bread-and-butter specimens. If you are just at the info gathering stage,
it would have been MUCH better, in my opinion, to REQUIRE private responses
to you instead of having them all go the listserver. You've already
required vendors to remain silent!

Ron



Ron Anderson, IBM, Hopewell Jct., New York, USA. anderron-at-us.ibm.com

IBM Analytical Services; http://www.chips.ibm.com/services/asg



"Gary Lovell" {gllovel-at-ppco.com} on 09/20/2000 04:22:52 PM

To: Microscopy-at-sparc5.microscopy.com
cc:


I would be interested in heaing from anyone who has done a market survey on
JEOL 8200 versus CAMECA SX100 microprobes. Which ( in the responders
opinion ) has the better system for geological quantitative WDS analysis,
or performs better in the SEM mode. Please give the reasons behind your
preference, if at all possible. PLEASE, NO VENDOR RESPONSE.

Gary L. Lovell
Phillips Petroleum Company
245a GB
Bartlesville, Ok. 74004
Phone: (918)661-9691
Fax : (918)662-2047
Email: gllovel-at-ppco.com








From daemon Thu Sep 21 11:01:12 2000



From: Tobias Baskin :      BaskinT-at-missouri.edu
Date: Thu, 21 Sep 2000 10:52:37 -0500
Subject: Re: Immuno TEM

Contents Retrieved from Microscopy Listserver Archives
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Greetings,
Paul Webster asks:
Why then should we decide it is important to have good morphology
at the EM level?

One answer is that the questions at light and electron levels
are distinct. At the light level, we might interested in finding out
what tissue expresses a particular antigen, whereas at the electron
level, we might be wondering whether it is in the outer or the inner
membrane of the mitochondrion. In the latter case, if we can't
distinguish outer from inner membrane, let alone a mitochondrion from
a lysosome, we are in trouble.

Tobias
--
_ ____ __ ____ Tobias I. Baskin
/ \ / / \ / \ \ University of Missouri
/ | / / \ \ \ Biological Sciences
/_ / __ /__ \ \ \__ 109 Tucker Hall
/ / / \ \ \
Columbia, MO 65211-7400 USA
/ / / \ \ \ voice:
573-882-0173
/ /____ / \ \__/ \____ fax: 573-882-0123



From daemon Thu Sep 21 11:08:47 2000



From: Tobias Baskin :      BaskinT-at-missouri.edu
Date: Thu, 21 Sep 2000 11:00:03 -0500
Subject: Re: Immuno TEM

Contents Retrieved from Microscopy Listserver Archives
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Greetings,
Paul Webster asks: Why then should we decide it is
important to have good morphology
at the EM level? snip snip

One answer is that the questions at light and electron levels
are distinct. At the light level, we might interested in finding out
what tissue expresses a particular antigen, whereas at the electron
level, we might be wondering whether it is in the outer or the inner
membrane of the mitochondrion. In the latter case, if we can't
distinguish outer from inner membrane, let alone a mitochondrion from
a lysosome, we are in trouble.

Tobias
--
_ ____ __ ____ Tobias I. Baskin
/ \ / / \ / \ \ University of Missouri
/ | / / \ \ \ Biological Sciences
/_ / __ /__ \ \ \__ 109 Tucker Hall
/ / / \ \ \
Columbia, MO 65211-7400 USA
/ / / \ \ \ voice:
573-882-0173
/ /____ / \ \__/ \____ fax: 573-882-0123



From daemon Thu Sep 21 12:20:23 2000



From: NPGSlithography-at-aol.com
Date: Thu, 21 Sep 2000 13:11:44 EDT
Subject: Re: mu-metal

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Dear Mark,

Years ago while doing lithography using a JEOL 840A, we observed definite*
line frequency interference in some of the patterns. The source of the noise
was traced to a power bus running in the ceiling to another lab, which could
not easily be changed.

JEOL provided a mu-metal shield on a trial basis that was specifically made
for the 840. As I recall, their price (~10 years ago) was $6k US. We could
quantitatively measure the change in the patterns and found that the
interference was reduced by about a factor of 3. Note that this shield
surrounded just the lower part of the column, i.e., it extended just above
the sample chamber.

With that said, I will now add that I agree with the other responses that
suggest the external noise may not be your primary problem. In my
experience, I have not seen that line frequency interference causes "fuzzy"
focus and astigmatism problems. Since the SEM scan is (or should be) in sync
with the line frequency, line frequency interference problems can show up as
localized distortions in the image position, depending on the scan frequency.

There are so many possible reasons for fuzzy focus and astigmatism problems
(as other responses have pointed out), that it is hard to suggest where you
should start. I think you could get better suggestions if you could describe
the recent history of the SEM related to its performance. For example, has
the SEM always been in its present location and has it always had this
problem? If it was moved to the room with the interference, did it work
properly before this? What is the best performance that has been seen on
your 5410? Have you contacted other 5410 users to check on their
experiences? (JEOL should have a user list. If not, I have two customers
with 5400's that may be able to offer some guidance.) Do you have an
experienced SEM operator who can work with your SEM and diagnose the
problems? (I know you have JEOL working on it, but while many SEM
technicians - from all SEM vendors - are very good, there are always those
who are just learning or just don't really care. Also, as pointed out
previously, since your room is out of spec, they have an easy excuse and may
not really be trying to solve the problem.)

Joe
_________________________________________
Joe Nabity, Ph.D.
JC Nabity Lithography Systems
E-Beam Lithography using Commercial SEMs & STEMs
PO Box 5354, Bozeman, MT 59717 USA
Voice: (406) 587-0848
FAX: (406) 586-9514
E-mail: info-at-jcnabity.com
Web: www.jcnabity.com

(*When doing lithography, you can often measure the frequency of the
interference in the patterns. Not that it really helps you, but if you are
interested, example lithography patterns with line frequency noise problems
can be seen at "www.jcnabity.com/pictures.htm" under the Diagnostic Images
heading.)



From daemon Thu Sep 21 12:26:38 2000



From: Lesley Weston :      lesley-at-interchange.ubc.ca
Date: Thu, 21 Sep 2000 10:19:28 -0700 (PDT)
Subject: Re: Citrated Saline

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The way I use it for Trypsin:

Trisodium citrate (Na3C6H5O7.2H2O), 4.4gm
Potassium chloride 10.0gm

To 1 litre with deionised water, pH to 7.8 with hydrochloric acid.

Hope this helps.

Lesley Weston.



On Wed, 20 Sep 2000, Ronald LHerault wrote:

} ------------------------------------------------------------------------
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} -----------------------------------------------------------------------.
}
}
} One of our students needs a receipe for citrated saline and cannot find it
} in the very limited references we have available here (a materials lab
} where one group is doing some tissue culture). She has seen it mentioned
} in articles but it must be so basic (or maybe acidic) that no one tells
} how it is made. I hope someone can enlighten us.
}
} Thanks.
}
} Ron
} lherault-at-bu.edu
}
}
}
}




From daemon Thu Sep 21 12:26:42 2000



From: Aviles, Phil :      paviles-at-dps.state.nm.us
Date: Thu, 21 Sep 2000 11:19:37 -0600
Subject: RE: What is GFP?

Contents Retrieved from Microscopy Listserver Archives
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I must have missed the original question, but Birefringence is the
difference between the two
refractive indices displayed by an anisotropic substance under polarized
light observation.

Phil Aviles
Forensic Microanalyst

} -----Original Message-----
} From: Nelson Conti [SMTP:NelsonC51-at-excite.com]
} Sent: Thursday, September 21, 2000 12:50 AM
} To: Leigh Ann
} Cc: Microscopy-at-sparc5.microscopy.com
} Subject: Re: What is GFP?
}
} ------------------------------------------------------------------------
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}
}
}
} On Wed, 20 Sep 2000 20:26:39 -0500, laniven-at-julian.uwo.ca wrote:
}
} |
} ------------------------------------------------------------------------
} | The Microscopy ListServer -- Sponsor: The Microscopy Society of America
}
} | To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} | On-Line Help
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} |
} -----------------------------------------------------------------------.
}
} |
} | Email: laniven-at-julian.uwo.ca
} | Name: Leigh Ann
} | School: UWO
} | Question: What is GFP? How do you convert a microscope for
} birefringence
} | and what type of microscopy is this?
} |
}
}
} Hi Leigh Ann -
} I can answer the first part of your question (to some
} extent), but I'm not
} very sure about the second part.
} GFP = Green Fluorescent Protein ; it's a protein that
} naturally
} fluoresces, and I believe that it was first discovered as a natural
} product
} from some marine animal (name ?). What I know of GFP is that it's gaining
} usage as a fluorescent marker. I believe it's been used as a marker for
} determining the location of X compound in a cell when viewed by
} fluorescent
} microscopy, but I'm sure that other usages have been cited in other
} research
} papers.
} As for the second part ... someone in this list may correct
} me, but I
} believe that birefringence is correlated with polarized light. You'll
} definitely need to consult with someone else in this list to get more
} information.
} Good luck getting more answers.
} Nelson Conti
}
}
}
}
}
} _______________________________________________________
} Say Bye to Slow Internet!
} http://www.home.com/xinbox/signup.html
}



From daemon Thu Sep 21 12:34:44 2000



From: Rosemary Walsh :      rw9-at-psu.edu
Date: Thu, 21 Sep 2000 13:57:54 -0400
Subject: Assessment of automated and semi-automated CPD's

Contents Retrieved from Microscopy Listserver Archives
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Dear Listers,
I am seeking input from those of you who use an
automated critical point dryer. The chamber on all of those
I've considered is much smaller than that of the Polaron E3000
which I have used for many years. What are the advantages
of automated / semi-automated critical point dryers?
Rosemary

Rosemary Walsh, Manager
The Electron Microscope Facility for the Life Sciences,
A Shared Technology Facility, The Life Sciences Consortium
1 South FrearLab
Penn State University
University Park, PA 16802
(814) 865-0212
rw9-at-psu.edu
http://www.lsc.psu.edu/stf/em/home.html





From daemon Thu Sep 21 13:28:58 2000



From: MICHAEL DELANNOY :      delannoy-at-mail.jhmi.edu
Date: Thu, 21 Sep 2000 14:21:50 -0400 (EDT)
Subject: Mitsubishi papaer

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Hello,
I am looking for a supplier of Mitsubishi thermal dye-sub paper
(with ribbon) catalog CK 200sa for printer. I was supplied by Kipp and
Sons, apparently now out of business. Preferable that supplier be
close to DC Baltimore region. Thank You.

Mike D.




From daemon Thu Sep 21 14:18:04 2000



From: Kristen A. Lennon :      kalen-at-iastate.edu
Date: Thu, 21 Sep 2000 14:09:34 CST6DST
Subject: formaldehyde nomenclature

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Hi All,
I have a picky question that I think would be good for all of us to be clear on,
so I'll ask. Can someone define the difference between formaldehyde and
formalin? My impression has always been that formalin is to formaldehyde as
Kleenex is to tissues (aka a type of brand). I have just come across a protocol
that specifically says "formalin (not formaldehyde)".
Thanks for your help,
Kristen


Kristen A. Lennon
Department of Plant Pathology
351 Bessey Hall
Iowa State University
Ames, IA 50011
email:
kalen-at-iastate.edu




From daemon Thu Sep 21 16:13:22 2000



From: Miser, Don E. :      Donald.E.Miser-at-pmusa.com
Date: Thu, 21 Sep 2000 17:03:18 -0400
Subject: RE: What is GFP?

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Leigh Ann,

Observing birefringence is a very useful means for identifying and locating
optically anisotropic materials, i.e. crystalline and polymeric materials.
For example, oxalates in tissues would display obvious birefringence,
whereas the rest of the tissue would not and would appear black with the
polarizer and analyzer inserted (termed "crossed nicols"). See the response
below from Olley for setup, but I would recommend the purchase of a
polarizing (transmitted light) microscope rather than converting something.
The "pretty interference colors" are termed birefringence and should be
viewed without the use of the condenser lens. Conoscopic light can be used,
however, for such things as the determination of the orientations of the
maximum and minimum refractive indices, the magnitude of the anisotropy, and
the orientation of the optic axes. These are best determined by observing
the interference figure that forms above the objective lens with the nicols
crossed and a Bertrand lens. A retardation plate (gypsum, mica, or quartz
wedge) is often inserted between the sample and the analyzer. If you don't
have a Bertrand lens, simply remove the ocular to view the figure (be sure
that the condenser inserted).

All this seems totally unrelated to GFP. You can put a UV source on a
polarizing light microscope if necessary.

Donald Miser

} -----Original Message-----
} From: Robert H. Olley [SMTP:r.h.olley-at-reading.ac.uk]
} Sent: Thursday, September 21, 2000 9:14 AM
} To: laniven-at-julian.uwo.ca
} Cc: Microscopy-at-sparc5.microscopy.com
} Subject: Re: What is GFP?
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} Leigh Ann,
}
} Birefringence is observed under a light microscope by putting a polarizer
} before the specimen (and generally before the condenser) and a similar
} device called the analyser between the specimen and the eyepiece: the
} analyser is usually set at 90^ to the polarizer. If the crystal (or even
} a piece of stretched plastic or a blob of liquid crystal) has different
} refractive indices in different directions, the polarized light passing
} through the specimen gets divided into two out-of-phase components which
} are recombined in the analyzer to give pretty interference colours, from
} which one can also make quantitative deductions. The people that use this
} technique most are geologists and mineralogists looking at thin polished
} rock sections, but we plastics people also look at polymers in this way.
} I'm not sure if it's used much in bio/med applications.
}
} A good book to explain the physics and applications of the technique is:
}
} Wood, Elizabeth Armstrong
} Crystals and light.
}
} while there are many books which concentrate more on the setup of the
} microscope. Here's a selection out of our library catalogue:
}
} Hartshorne, N.H.
} Crystals and the polarising microscope.
}
} Polarized light microscopy by Walter C. McCrone, Lucy B.McCrone
} and John Gustav Delly
}
} The polarizing microscope by A. F. Hallimond
} Hallimond A.F.
}
}
} +------------------------------------------------------------------------+
} | Robert H.Olley Phone:
} |
} | J.J.Thomson Physical Laboratory {direct line +44 (0) 118 9318572
} |
} | University of Reading {University internal extension 7867
} |
} | Whiteknights Fax +44 (0) 118 9750203
} |
} | Reading RG6 6AF Email: R.H.Olley-at-reading.ac.uk
} |
} | England URL: http://www.reading.ac.uk/~spsolley
} |
}
} +------------------------------------------------------------------------+
}
} } Email: laniven-at-julian.uwo.ca
} } Name: Leigh Ann
} } School: UWO
} } Question: What is GFP? How do you convert a microscope for birefringence
} } and what type of microscopy is this?



From daemon Thu Sep 21 16:29:00 2000



From: Michael Reiner :      Elektronenmikroskopie-at-web.de
Date: Thu, 21 Sep 2000 23:21:25 +0200
Subject: Re: Immuno TEM

Contents Retrieved from Microscopy Listserver Archives
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Hello everyone on the list who is doing Immuno-EM!
Dear Greg, Paul, Tom and Tim

I have read your contributions with great interest and smiled everytime.
Ok, since my bible in Immunoelectronmicroscopy is the outstanding book of G.Griffiths ("Fine structure immunocytochemistry", 1993), and as I am trying to do good IEM for over three years, I do agree with all of you.

First, let me state that it is particulary difficult to enable monoclonal antibodies for EM immunoprocedures. In fact, I was not able to run a good EM-Immuno with a monoclonal one (but the polyclonals do work.). And I agree with you, most of the (monoclonal) antibodies provided today are very well suited for Western blotting and sometimes even for LM because they were fitted for degenerative conditions applied in these techniques. I am always aware of that approximately 90% of the epitopes are gone, due to embedding and sectioning.

Second, many collegues who do have quite satisfactorily results at LM ask me why, the signal at EM is not worth looking at, to poor. Many of them are not aware of the dimensions we are talking about, a few milimetres in square and a section thickness of 50-70 nms is little, and fixation and post-processing leads to a loss of antigenicity.

Third, if you do have an antibody running well in IEM, use it. There are, in fact too few AB suitable for IEM. I am wondering why there isn´t any company already which is offering AB´s for IEM which recognize their epitope after aldehyde degeneration/fixation and acrylic plastic embedding.

At least, counting your signal should help. Sometimes you don´t recognize specific signals because you only see almost nothing at a magnification of x20,000 or more. If you start counting more fields, you are often surprised by the high specifity, althoug it´s very weak.

So, my two cents to this topic are: Try different antibodies from different Labs made against the same epitope! If it works, you should always use it as reference marker. And don´t forget: IEM does take so much more time and spirit as LM, this business is more tricky.

The best wishes for the IEM´s on this list.

Michael Reiner

PS: In memoriam Hildy Crowley, I already do miss her reply to this topic.
_______________________________________________________________________
1.000.000 DM gewinnen - kostenlos tippen - http://millionenklick.web.de
IhrName-at-web.de, 8MB Speicher, Verschluesselung - http://freemail.web.de





From daemon Thu Sep 21 16:29:43 2000



From: John J. Bozzola :      bozzola-at-siu.edu
Date: Thu, 21 Sep 2000 16:22:41 -0500
Subject: Re: formaldehyde nomenclature

Contents Retrieved from Microscopy Listserver Archives
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Formaldehyde is a gas which may be dissolved in water.

A 40% solution of formaldehyde dissolved in water is called Formalin.
Normally, various additives are put into the 40% solution of
formaldehyde in order to stabilize it (I believe that traces of
methanol may be added). Because of impurities, Formalin is usually
considered unsuitable for electron microscopy studies. Confusion
often arises when people refer to percentages of these solutions. For
example, a 10% solution of formaldehyde is quite different than a 10%
solution of Formalin ((10% versus 4%, respectively).

Hope this answers your question.


} I have a picky question that I think would be good for all of us to
} be clear on,
} so I'll ask. Can someone define the difference between formaldehyde and
} formalin? My impression has always been that formalin is to formaldehyde as
} Kleenex is to tissues (aka a type of brand). I have just come across
} a protocol
} that specifically says "formalin (not formaldehyde)".

####################################################################
John J. Bozzola, Ph.D., Director
Micro-Imaging and Analysis Center
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
####################################################################



From daemon Thu Sep 21 17:01:48 2000



From: Geoff McAuliffe :      mcauliff-at-UMDNJ.EDU
Date: Thu, 21 Sep 2000 17:55:44 -0400
Subject: Re: formaldehyde nomenclature

Contents Retrieved from Microscopy Listserver Archives
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"Kristen A. Lennon" wrote:

} Hi All,
} I have a picky question that I think would be good for all of us to be clear on,
} so I'll ask. Can someone define the difference between formaldehyde and
} formalin? My impression has always been that formalin is to formaldehyde as
} Kleenex is to tissues (aka a type of brand). I have just come across a protocol
} that specifically says "formalin (not formaldehyde)".
} Thanks for your help,
} Kristen
}
} Kristen A. Lennon
} Department of Plant Pathology
} 351 Bessey Hall
} Iowa State University
} Ames, IA 50011
} email:
} kalen-at-iastate.edu

Hi Kristen:

Formaldehyde is a gas, when bubbled through water the solution becomes saturated
to about 37-40%. Methanol is usually added as a stabilizer. Formalin is the name
often given to a 1:9 dilution of the "formaldehyde" solution, it is about 3.7-4%
formaldehyde. The nomenclature is not exact, to put it mildly. To know exactly the
person who wrote the protocol means, you will probably have to ask him/her. I don't
know how you could have formalin without formaldehyde!

Geoff
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************





From daemon Thu Sep 21 17:06:55 2000



From: Tom Phillips :      PhillipsT-at-missouri.edu
Date: Thu, 21 Sep 2000 16:57:26 -0500
Subject: Re: formaldehyde nomenclature

Contents Retrieved from Microscopy Listserver Archives
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I concur with everything John said and point out that one also
commonly sees 3.7% formalin in protocols. This is the "same" as 4%
usually since it depends on whether you mean % w/w or % w/v (i can't
remember which is which).

Also, besides the impurities, formalin is often bought in gallon or
barrel sizes so it can sit around a long time and form polymers not
present in freshly depolymerized paraformaldehyde.

So the words should never be taken as synonomous.

Tom




}
}
} Formaldehyde is a gas which may be dissolved in water.
}
} A 40% solution of formaldehyde dissolved in water is called
} Formalin. Normally, various additives are put into the 40% solution
} of formaldehyde in order to stabilize it (I believe that traces of
} methanol may be added). Because of impurities, Formalin is usually
} considered unsuitable for electron microscopy studies. Confusion
} often arises when people refer to percentages of these solutions.
} For example, a 10% solution of formaldehyde is quite different than
} a 10% solution of Formalin ((10% versus 4%, respectively).
}
} Hope this answers your question.
}
} } I have a picky question that I think would be good for all of us to
} } be clear on,
} } so I'll ask. Can someone define the difference between formaldehyde and
} } formalin? My impression has always been that formalin is to formaldehyde as
} } Kleenex is to tissues (aka a type of brand). I have just come
} } across a protocol
} } that specifically says "formalin (not formaldehyde)".
}
} ####################################################################
} John J. Bozzola, Ph.D., Director
} Micro-Imaging and Analysis Center
} 750 Communications Drive - MC 4402
} Southern Illinois University
} Carbondale, IL 62901 U.S.A.
} Phone: 618-453-3730
} Fax: 618-453-2665
} Email: bozzola-at-siu.edu
} Web: http://www.siu.edu/departments/shops/cem.html
} ####################################################################

--
Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)



From daemon Thu Sep 21 17:20:59 2000



From: Donald Lovett :      lovett-at-tcnj.edu
Date: Thu, 21 Sep 2000 18:12:02 -0400
Subject: Re: formaldehyde nomenclature

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Formaldehyde is a gas. An aqueous solution saturated with the gas is
called formalin. This saturated solution is called 100% formalin, which
contains approximately 37% formaldehyde. Thus, a solution of 10%
formalin is very different in strength from a solution that is 10%
formaldehyde. Thus, an instruction may say to use "10% formalin (not
formaldehyde)" to make sure that you indeed are using the correct
strength.

DL

Kristen A. Lennon wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hi All,
} I have a picky question that I think would be good for all of us to be clear on,
} so I'll ask. Can someone define the difference between formaldehyde and
} formalin? My impression has always been that formalin is to formaldehyde as
} Kleenex is to tissues (aka a type of brand). I have just come across a protocol
} that specifically says "formalin (not formaldehyde)".
} Thanks for your help,
} Kristen
}
} Kristen A. Lennon
} Department of Plant Pathology
} 351 Bessey Hall
} Iowa State University
} Ames, IA 50011
} email:
} kalen-at-iastate.edu

--
____________________________________________________________________
Donald L. Lovett voice: 609-771-2876
Assoc. Professor fax: 609-637-5118
Dept. of Biology e-mail: lovett -at- tcnj.edu
The College of New Jersey
P.O. Box 7718 www.tcnj.edu/~scholars/lovett.html
Ewing, NJ 08628-0718



From daemon Thu Sep 21 17:27:18 2000



From: Robert Mixon :      mixonr-at-ohsu.edu
Date: Thu, 21 Sep 2000 15:20:10 -0700
Subject: Formalin

Contents Retrieved from Microscopy Listserver Archives
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Kristen: Formalin is commonly used to describe a mixture of37% to 38% formaldehyde in water (this reagent grade chemical is also called formaldehyde solution). This solution of dissolved formaldehyde gas generally contains some small amount of methanol in it also. Now for the good part! Traditionally this reagent grade mixture of the 37% or so formaldehyde in water is considered a 100%formalin concentration (the maximum concentration range you can buy commercially). A traditional 10% formalin solution would then contain about 3.7% to 3.8% aldehyde concentration. Many E-M folks prefer to make their formaldehyde solutions from the polymer paraformaldehyde--in part I think believing that there is less methanol in the final product yet folks have had success using both kinds in the right application. I hope this helps to clarify the common usage of these terms.




From daemon Thu Sep 21 18:11:39 2000



From: Michael Plociniak :      plocinia-at-aecom.yu.edu
Date: Thu, 21 Sep 2000 18:58:42 -0400
Subject: TEM in-situ hybridization

Contents Retrieved from Microscopy Listserver Archives
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Dear Listers,
For preembedment EM-ISH, is a two-step detection method more sensitive than
a single-step method? Specifically, I am planning to detect
digoxigenin-labeled RNA probes with mouse anti-dig antibody, followed by
anti-mouse gold-conjugated secondary antibody (then enhancing ultrasmall
gold particles). Or would direct detection of the dig probes with an
anti-dig gold-conjugated antibody lend any advantage? One review
attributes higher sensitivity for two-steps, but I think this might be
restricted to postembedment methods only (J. Histochem. Cytochem.
45:481-92,1997).

If a two-step method is used, would gold-conjugated antibodies (against
biotin probes) have advantages over streptavidin-gold? As recently
discussed on this list, unconjugated streptavidin will compete with the
gold-labeled streptavidin, thus reducing signal strength. Nanoprobes makes
covalently bound gold antibodies, but I have seen a higher background with
these than with noncovalently bound antibodies, at least in cultured rat
neurons.

Finally, will glutaraldehyde levels greater than 0.05% tend to inhibit
hybridization? Rat glioma monolayers have been labeled with EM-ISH by
Punnonen, et al., using 4% paraformaldehyde and 0.05% glut for 30 min. (J.
Histochem. Cytochem. 47:99-112, 1999). However, this protocol included
0.1% saponin permeabilization for 30 min. Their probes were up to 850 bp
and "partially hydrolyzed" while my probes are only 50 bp long. I have
immunolabeled neurons fixed in 2% glut with no permeabilization; so if the
antibodies can make it into the cells, then the smaller RNA probes should
too, right?

I know that target abundance is an issue here. I plan to include positive
controls for abundant RNAs (e.g., beta-actin), and I will consider Tyrimide
Signal Amplification, if necessary.

Thank you for your helpful comments on any of these questions. I will be
happy to elaborate if this inquiry is too brief.


Michael Plociniak
Research Technician
Albert Einstein College of Medicine
Neuroscience Dept.
Rose Kennedy Center, 529
Bronx, NY 10461
(718) 430-3509
plocinia-at-aecom.yu.edu

transmission and scanning electron microscopy




From daemon Thu Sep 21 18:41:31 2000



From: JoAnn Buchanan :      redhair-at-leland.Stanford.EDU
Date: Thu, 21 Sep 2000 18:33:46 -0500
Subject: immuno TEM

Contents Retrieved from Microscopy Listserver Archives
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Dear all,


Haven't heard this approach mentioned yet.


Win95 has a utility named system monitor (Start | Programs | Accessories |
System Tools |). I don't believe it's installed by default. You may have
to add it through the custom install section. It could help you rule out
the physical disk, CPU, and RAM as bottlenecks. You might compare the
charts using your different printers. It's a "watch it in real time"
affair though you might be able to use {Alt-Print Screen} to get a snapshot
for comparison or baselining (it does work, I tried it : ) ).


It contains counters to monitor your CPU, physical disk, virtual memory,
and network connections. A knowledgeable person in your computer
department should be able to help you sort which counters might be useful
and what they mean. Or you could play with different counters.
Unfortunately the help portion of the utility is the stuff of legends.


I'd probably start with Kernel Processor Usage %, File System Bytes
Written/Second & Dirty Data (This sounds like the amount of queued data),
and Memory Manager Disk Cache Size & Page Faults.


Hope it helps,


Chuck
-------------------------------------
Name: Charles Gilbert VOC: (704) 355-5261
Carolinas Medical Center FAX: (704) 355-8424
Dept of Pediatric Research digPager: (704) 355-4088 : 2058
PO Box 32861
Charlotte, NC 28232-2861




Please keep the discussion coming! I have found it extremely informative
and helpful, as I am attempting pre-embedding anti-GFP immunolabelling with
nanogold and silver enhancement. ImmunoEm is so important now that many
people have localized their GFP transfected proteins by confocal. They want
to know what are the fluorescent blobs they cannot quite resolve. Before I
attempt any immunoEm with anyone, they must do a fixation series of
formaldehyde and glutaraldehyde dilutions to see where they lose their
fluorescent signal. We attempt to get the best fixation possible, yet
penetration of the ab is an issue too. It is always a compromise. There are
many ways to skin a cat, and only time and effort will hopefully give you
an answer.
JoAnn Buchanan
Stanford University School of Medicine
Stanford, CA





From daemon Thu Sep 21 19:14:17 2000



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Fri, 22 Sep 2000 12:12:27 GMT+1200
Subject: Re: formaldehyde nomenclature

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} I have a picky question that I think would be good for all of us to be clear on,
} so I'll ask. Can someone define the difference between formaldehyde and
} formalin? My impression has always been that formalin is to formaldehyde as
} Kleenex is to tissues (aka a type of brand). I have just come across a protocol
} that specifically says "formalin (not formaldehyde)".
} Thanks for your help,
} Kristen


As I have understood it, formalin is the (fairly concentrated)
aqueous solution, formaldehyde is the actual pure compound HCHO.

Not that I've ever used, the stuff, though.

cheers

rtch


Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand



From daemon Thu Sep 21 19:58:35 2000



From: Paul Webster :      pwebster-at-mailhouse.hei.org
Date: 21 Sep 00 17:56:55 -0700
Subject: Re: Immuno TEM

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------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Reply to: Re: Immuno TEM
Tobias Baskin writes: One answer is that the questions at light and electron levels are distinct. At the light level, we might interested in finding out what tissue expresses a particular antigen, whereas at the electron level, we might be wondering whether it is in the outer or the inner membrane of the mitochondrion. In the latter case, if we can't distinguish outer from inner membrane, let alone a mitochondrion from a lysosome, we are in trouble.


Hi Tobias,

I agree totally. The level of acceptance for poor morphology will depend on the result expected. Again, each system will have its own protocol for getting that particular result. I am not encouraging everyone to go out and prepare samples with such poor morphology that any result they get will be meaningless. This would be irresponsible for me to advocate and a waste of time for anyone who was foolish enough to follow this advice.

Rather, I suggest that if it is possible to label purified (or semi-purified) sub-cellular particles, then do that. If you are looking for antigens on the outside of mitochondria, perform a pre-embedding labeling on purified fractions. As the mitochondria are so recognizable, it is not even necessary to get very pure fractions. If the antigen is inside the mitochondria, then sectioning is probably the best way. But even then, if the profile that is labeled is so obviously a mitichondion, then it doesn't matter if the cristae are not perfectly preserved (unless there is extraction or redistribution of antigen). If specific membrane markers are available, structural morpholgy is even less important. Co-localizing the marker with the test antibody on a membrane or organelle fragment is often very acceptable.
Sometimes semi-purified fractions can be obtained just by breaking open cells to let out the antigens we are not interested in (unless the antigens are cytoplasmic of course). Gently lysed cells have wonderful contrast and it is possible to recognize a great number of the intracellular organelles if they have been fixed correctly. The added accessibility obtained by removing the cytoplasm makes it possible to reach antigens that are usually covered. I know an investigator who labeled the lumen of the RER with a very specific antibody. He couldn't do this on well fixed, heathly cells as the antibody could not gain access to the luminal antigens. He solved his problem by noticing that some of the cells in the pellet had obviously died before being fixed, and had really poor morphology. However, the RER was easily recognizable and had lots of gold particles in the swollen lumen. Is this unacceptable because the cells were not perfectly preserved? The journal reveiwers didn't think so.

I think that immunocytochemistry is a very special branch of EM that is almost impossible to provide as a service. I know that this is what is being asked of EM labs all over the world and I know it will be impossible to change this. However, it is our responsibility to educate our colleagues and to allow them to become involved in the discovery process of how their antibodies work. I have no good solution to this, but do know that if someone is involved in their own specimen preparation and data collection, their work will progress much faster. If new EM users are taught why particular approaches should be applied instead of being given one protocol to apply exactly as written, pretty neat ideas originate from their work.

We must stop being the "black box" of science.
Regards,

Paul Webster.

Reccommended reading: G. Griffiths 1993 Fine Structure Immunocytochemistry. Springer Verlag, Heidelberg & Berlin
{http://www.amazon.com/exec/obidos/ASIN/038754805X/qid%3D969583271/102-2138449-1518557}

Discalimer: I have no financial interest in Amazon or Springer. I have co-authored papers with Griffiths and we teach immunocytochemical methods together.


Paul Webster, Ph.D
House Ear Institute
2100 West Third Street
Los Angeles, CA 90057
phone:213 273 8026
fax: 213 413 6739
e-mail: pwebster-at-hei.org
http://www.hei.org/htm/aemi.htm

Tobias Baskin wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Greetings,
} Paul Webster asks: Why then should we decide it is } important to have good morphology
} at the EM level? snip snip
}
} One answer is that the questions at light and electron levels } are distinct. At the light level, we might interested in finding out } what tissue expresses a particular antigen, whereas at the electron } level, we might be wondering whether it is in the outer or the inner } membrane of the mitochondrion. In the latter case, if we can't } distinguish outer from inner membrane, let alone a mitochondrion from } a lysosome, we are in trouble.
}
} Tobias
} -- } _ ____ __ ____ Tobias I. Baskin
} / \ / / \ / \ \ University of Missouri
} / | / / \ \ \ Biological Sciences
} /_ / __ /__ \ \ \__ 109 Tucker Hall
} / / / \ \ \ } Columbia, MO 65211-7400 USA
} / / / \ \ \ voice: } 573-882-0173
} / /____ / \ \__/ \____ fax: 573-882-0123
}
}
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From daemon Thu Sep 21 22:51:38 2000



From: Rosemary White :      Rosemary.White-at-pi.csiro.au
Date: Fri, 22 Sep 2000 14:18:25 +1000
Subject: Re: JEOL vs CAMECA

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Another thing that can be critical is service from the local reps in your
area. This varies enormously around the globe and can also vary between
different regions of a country. Ask others in your area what the service
has been like over the last few years. If you've got a complex piece of
machinery you'll appreciate good service, especially as it ages.

Rosemary


Rosemary White
Microscopy Centre
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia

phone 61-2-6246 5475
fax 61-2-6246 5000
email r.white-at-pi.csiro.au





From daemon Fri Sep 22 06:26:31 2000



From: didier goux :      didier.goux-at-unicaen.fr
Date: Fri, 22 Sep 2000 13:13:57 -0700
Subject: Re: Dye Sub printer speeds- followup

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Hello


laser printer have memory that could be enhanced

I don't know if it is valuable for dye sub printer but it could accelerate
the printing process

Bye

Didier


} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America



} } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } }
Didier Goux
CENTRE DE MICROSCOPIE ELECTRONIQUE
Université de CAEN Tel : (33) 02.31.56.58.13
Campus I Fax : (33) 02.31.56.56.00
Esplanade de la Paix 14032 CAEN CEDEX FRANCE
mailto:didier.goux-at-unicaen.fr








From daemon Fri Sep 22 07:15:13 2000



From: dgambaro-at-memc.it
Date: Fri, 22 Sep 2000 14:03:33 +0200
Subject: Help for LaB6 filament

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Dear all,
we have received a SEM Topcon SM510 and we would like to upgrade this
system with LaB6 filament.
It is clear that the system is almost ready for this upgrade but we have a
lot of problems to obtain information from Topcon in Europe (our SEM is in
Italy).
We need help to know the ion pump necessary to upgrade our system, how to
install it and the procedures to convert the SEM for LaB6 filament use.
We need also one complete copy of Topcon SM510 manual in order to clearly
understand how to correctly work with LaB6 filament.

Thanks

Daniela




From daemon Fri Sep 22 09:09:04 2000



From: Kristen A. Lennon :      kalen-at-iastate.edu
Date: Fri, 22 Sep 2000 08:58:46 CST6DST
Subject: formaldehyde/formalin

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Hi All,
Thanks to all of you who responded to my query. It was a question that had been
sitting in the back of my mind for several years (since the time a mentor of
mine told me that formalin was a "safer" alternative to formaldehyde as my
fellow students and I proceeded to be pretty much covered with it while doing
some mass field fixes). When that protocol hit, it was a good excuse to settle
it once and for all!
Thanks,
Kristen


Kristen A. Lennon
Department of Plant Pathology
351 Bessey Hall
Iowa State University
Ames, IA 50011
email:
kalen-at-iastate.edu




From daemon Fri Sep 22 10:04:27 2000



From: Timothy Schneider :      Timothy.Schneider-at-Mail.TJU.EDU
Date: Fri, 22 Sep 2000 10:50:57 -0400
Subject: MORE IEM

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Hi Folks:

I am writing in response to Paul Webster's recent description of gold
labeling the lumen of RER by way of lyseing cells and isolating the
resulting membranes and vesicles in a fraction. The basic question I have
is, at what point was the fraction exposed to the primary antibody and the
secondary antibodie that would have been conjugated to colloidal gold? Did
these steps occur before any fixation? Was the fraction fixed, dehydrated,
embedded, thin sectioned and then exposed to the primary and secondary
antibodies? Or was a entirely different procedure used.

For the past 9 years I have been working with Plasmodium falciparum during
its 48 hour asexual life cycle in human erythrocytes. I have been able to
isolate various membranous structures which we believe contain proteins that
are of interest to us. We have had only marginal success in labeling said
structures in lightly fixed, L. R. White embedded sections. I might be able
to adept your mentioned RER fractionation technique to meet my needs.
Thanks in advance for any help, Tim

Timothy G. Schneider
Director of Electron Microscopy
Department of Pathology
Room 229 Jefferson Hall
Thomas Jefferson University
1020 Locust St.
Philadelphia Pa. 19107
215-503-4798 work
610-613-8170 cellular
timothy.schneider-at-mail.tju.edu




From daemon Fri Sep 22 12:42:30 2000



From: Paul Webster :      pwebster-at-mailhouse.hei.org
Date: 22 Sep 00 10:22:05 -0700
Subject: RE: TEM in-situ hybridization

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From: Paul Webster :      pwebster-at-mailhouse.hei.org
Date: 22 Sep 00 10:22:05 -0700
Subject: RE: TEM in-situ hybridization

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The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Reply to: RE: TEM in-situ hybridization



Michael Plociniak wrote:
} } Dear Listers,
} For preembedment EM-ISH, is a two-step detection method more sensitive than
} a single-step method? Specifically, I am planning to detect
} digoxigenin-labeled RNA probes with mouse anti-dig antibody, followed by
} anti-mouse gold-conjugated secondary antibody (then enhancing ultrasmall
} gold particles). Or would direct detection of the dig probes with an
} anti-dig gold-conjugated antibody lend any advantage? One review
} attributes higher sensitivity for two-steps, but I think this might be
} restricted to postembedment methods only (J. Histochem. Cytochem.
} 45:481-92,1997).

Hi Michael,

As I have been getting lots of off-line e-mail encouraging me to continue writing, I will attempt to offer my thoughts on your question for all to see. As you know, in situ hybridization is just another form of affinity localization labeling protocol. In this instance the probe is a nucleic acid sequence instead of an antibody. Much the same rules apply. I certainly don't know all the answers but can share some of what I have learned the hard way. I have embedded my comments in your text. :

Two step, sequential detection methods (primary label, followed by secondary with attached visualization probe) are usually more sensitive. This was first observed by Coons in the 1950's, and he is generally considered to be the father of immunoctyochemistry. This sequential labeling can be applied to any labeling system you want to use. You must be careful to differentiate between signal amplification and sensitivity. What you (and all of use) are looking for is to maximize the amount of target that reacts with affinity marker (increasing sensitivity). Signal amplification refers to the process of making the reacted agents more visible. }
} If a two-step method is used, would gold-conjugated antibodies (against
} biotin probes) have advantages over streptavidin-gold? As recently
} discussed on this list, unconjugated streptavidin will compete with the
} gold-labeled streptavidin, thus reducing signal strength. Nanoprobes makes
} covalently bound gold antibodies, but I have seen a higher background with
} these than with noncovalently bound antibodies, at least in cultured rat
} neurons.

If you are using antibodies to digoxigenin, then a secondary antibody-gold probe should be sufficient. In your system this would be mouse anti-digoxigenin followed by anti-mouse-gold. Remeber that if you choose to use nanoprobes, it might be difficult to later perform multiple labeling experiments. If you want to look for biotin labeled nucleic acid probes try using anti-biotin antibodies and cold water fish skin gelatin as a blocking agent - not serum.
}
} Finally, will glutaraldehyde levels greater than 0.05% tend to inhibit
} hybridization? Rat glioma monolayers have been labeled with EM-ISH by
} Punnonen, et al., using 4% paraformaldehyde and 0.05% glut for 30 min. (J.
} Histochem. Cytochem. 47:99-112, 1999). However, this protocol included
} 0.1% saponin permeabilization for 30 min. Their probes were up to 850 bp
} and "partially hydrolyzed" while my probes are only 50 bp long. I have
} immunolabeled neurons fixed in 2% glut with no permeabilization; so if the
} antibodies can make it into the cells, then the smaller RNA probes should
} too, right? }
The only answer to this question is what you will tell us when you have performed the experiment. Each system has its own unique qualitites and sensitivity to fixatives will be one of these variables. If it bothers you to include glutaraldehyde then leave it out. Fix with a stong glutaraldehyde solution after you have performed your hybridization and silver enhancing steps.

Watch out for the mistake that many people make in assuming that immunoreagents are inert objects and that their size is the only property to be taken into account. As someone who mailed me said: immunoreagents are biological molecles and not chemicals. They are charged molecules and have many complex, variable interactive and binding properties that we cannot begin to know. Try out your protocol and see if it works. If is does then the question about your RNA probe is answered.

} I know that target abundance is an issue here. I plan to include positive
} controls for abundant RNAs (e.g., beta-actin), and I will consider Tyrimide
} Signal Amplification, if necessary.

One possible step you could put in is to first try out your whole preparation and immunolabeling protocol at the LM level. Use the same reagents and same preparation protocols as you plan to use for EM and expose to silver enhancment for a longer time so that you can see the signal by LM. If you see a signal then you will be confident that you will see a signal when you go to examine the EM experiment. You might even try to find a way of even preparing the LM sample that incorporates the resin embedding so that you can control for possible digestion of the silver signal by osmium tetroxide.

Regards,

Paul Webster

}
} Thank you for your helpful comments on any of these questions. I will be
} happy to elaborate if this inquiry is too brief.
}
}
} Michael Plociniak
} Research Technician
} Albert Einstein College of Medicine
} Neuroscience Dept.
} Rose Kennedy Center, 529
} Bronx, NY 10461
} (718) 430-3509
} plocinia-at-aecom.yu.edu
}
} transmission and scanning electron microscopy
}
Paul Webster, Ph.D
House Ear Institute
2100 West Third Street
Los Angeles, CA 90057
phone:213 273 8026
fax: 213 413 6739
e-mail: pwebster-at-hei.org
http://www.hei.org/htm/aemi.htm
}
}
}
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From daemon Fri Sep 22 13:20:59 2000



From: tellis2-at-hallmark.com
Date: Fri, 22 Sep 2000 13:09:55 -0500
Subject: TOPCON 500/510

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Daniela:
I have a TOPCON 500/510 wet SEM and I have the manual although I don't
know how to get a copy to you. I use the tungsten filament, don't have
theLaB6 filament. You might ask the people I have repair our SEM, they all
used to work for TOPCON and they have parts for them.

Chuck Humphreys
Image Control Inc.
P.O. Box 720596
Orlando, FL 32876-0596
U.S.A.
email : cchumph-at-ibm.net

Terry Ellis
Hallmark Cards Inc.
2501 McGee
Kansas City, MO 64141
U.S.A.
email: tellis2-at-hallmark.com




From daemon Fri Sep 22 14:50:19 2000



From: p.ingram-at-cellbio.duke.edu (peter ingram)
Date: Fri, 22 Sep 2000 15:36:19 -0400
Subject: Annual NC Microscopy Symp.

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THE
NORTH CAROLINA SOCIETY FOR MICROSCOPY
AND MICROBEAM ANALYSIS

present the

NINETEENTH ANNUAL
SYMPOSIUM ON
ADVANCES IN MICROSCOPY

"Molecules, Cells & Microchips"

Coastline Convention Center, Wilmington, North Carolina

October 13 - 15, 2000

FINAL ANNOUNCEMENT!

The Nineteenth Annual Symposium, sponsored by the North Carolina
Society for Microscopy and Microbeam Analysis (NCSMMA), has been planned
with a theme of "Molecules, Cells & Microchips" Continuing with the
tradition of the symposium, the guest lecturers are composed of both
nationally and internationally distinguished scientists.

Speakers who have agreed to participate (so far) this year include:

* Ian Anderson (ORNL, Oak Ridge, TN)Spectrum Imaging: Microanalysis for a
New Millennium
* Ken Downing (University of California, Berkeley, CA) High Resolution
Protein Structure
* Jan Ho (Johns Hopkins University, Baltimore, MD) Scanning Probe
Microscopy in Biology
* Stefan Jeglinski (4pi Analysis Inc.) Open Source software as a Business
Model for a Microscopy Company?
* Dan Kiehart (Duke University Medical Center, Durham NC) Flies 'R Us
* Richard Palmer (Duke Univ. NC) Recent Developments in Infrared Imaging
and Microscopy
* Peter So (MIT Cambridge, MA) New Microscopy Instrumentation for
Biomedical Research
* Rich Superfine (UNC, Chapel Hill, NC)Remote Nanomanipulation: from
Viruses to Nanotubes


* The meeting has several purposes, not the least of which is to
draw attention of the scientific community to emerging developments in the
practical and basic research aspects of exciting new fields, and to bring
people together from diverse disciplines to discuss how innovative
techniques will be relevant to the future direction of microscopy and
microprobe analysis. In particular, this year, special emphasis will be
placed on how recent advances in electron, optical and probe microscopies
have resulted in new knowledge that has benefited microscopy in general and
are having a significant impact in the biological and physical sciences.
The symposium also offers an opportunity for interested participants
including students to submit abstracts of related studies for poster
display.

* 3 special WORKSHOPS/TUTORIALS will be offered at NO ADDITIONAL
CHARGE to participants in the Symposium: (a) Immuno/Microwave EM
Techniques, (Cindy Hastings, VA Little Rock AR) (b) Atomic Force Microscopy
(Jan Ho) (c) Digital Imaging Methods (John Mackenzie, North Carolina State
Univ). These are practical, introductory sessions and no previous
experience or knowledge is necessary.

Registration Fees, Hotel rates

The $90 ($100 on site) per person and $50 for students ($60 on site).
Registration fee includes: symposium attendance and materials, Saturday
lunch, breaks, and Friday and Saturday evening meals. Additional Friday
evening tickets are available for Adults - $20; Children 10 years of age
and under - $10. Additional Saturday evening tickets are available for
Adults - $20; Children 10 years of age and under - $10. There is a $15
fee for all cancellations.

COAST LINE INN special rates $80/room/night (single or double). Tel: 1-800
617-7732

For questions or further information on Registration, please telephone
Betty Gooch, Duke University Medical Center: (919) 286-0411 x 6508 or
email: b.gooch-at-cellbio.duke edu

Website: http://152.3.167.174/NCAnnSymp2000.html

Or call Peter Ingram/Ann LeFurgey (919) 660-2671
email: p.ingram-at-cellbio.duke.edu







From daemon Fri Sep 22 15:12:26 2000



From: McLean, Dorrance :      dmclea-at-sandia.gov
Date: Fri, 22 Sep 2000 14:03:24 -0600
Subject: MAT: X-section of thin film on PMMA

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Dear All,

Today's question is, how will PMMA react in the PIPS? I have a thin film of
Ti/Cu/Ti grown on a substrate of (1 1/2um thick) PMMA . My plan is to do a
regular film to film x-section, dimple down to about 10um and finish it in
the PIPS...I'm just not sure how PMMA mills?

If anyone has done this sort of sampIe I would love to hear about it. As
always any suggestions are gladly received.

Thanks in advance for all your help.

Sincerely,
Dorrance




From daemon Fri Sep 22 17:01:00 2000



From: Carolyn.Gondran-at-SEMATECH.Org
Date: Fri, 22 Sep 2000 16:49:58 -0500
Subject: TEM sample prep position available - semiconductor materials

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Looking for a TEM sample Prep technician at SEMATECH in Austin TX

SEMATECH is a research and development consortium working on
advanced materials and processes for semiconductor manufacturing.

JOB RESPONSIBILITIES:
Prepare samples, both cross sectional and plan view,
of semiconductor material and devices for TEM analysis.
Using flat-polishing, wedge-polishing, mechanical dimpling,
ion milling, Focused Ion Beam milling and additional techniques as needed.
Maintain lab equipment in working order, maintain detailed records,
lab safety, housekeeping, order supplier and perform additional tasks as needed.
QUALIFICATIONS:
Two year technical degree, Superior fine motor skills and near vision,
Ability to work with limited supervision and direction,
Ability to multitask effectively, Strong organizational skills
Ability to work with limited supervision and direction,
Experience with sample polishing and the semiconductor industry is preferred
Experience with FIB and TEM prep greatly preferred

Interested parties should send resumes to:

Carolyn Gondran
2706 Montopolis Dr.
Austin TX 78741

Carolyn.Gondran-at-SEMATECH.org







From daemon Fri Sep 22 17:40:10 2000



From: michael shaffer :      epmalab-at-darkwing.uoregon.edu
Date: Fri, 22 Sep 2000 15:31:20 -0700
Subject: RE: Help for LaB6 filament

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Daniela writes ...

} we have received a SEM Topcon SM510 and we would like to
} upgrade this system with LaB6 filament.
} It is clear that the system is almost ready for this
} upgrade but we have a lot of problems to obtain
} information from Topcon in Europe (our SEM is in Italy).
} We need help to know the ion pump necessary to upgrade our
} system, ...

I believe an ion pump is definitely necessary ... LaB6 emitters are
extremely susceptable and unstable with respect to contamination.
For adding an ion pump, you will need (1) the ion pump itself and its
power supply ... (2) and you'll need add a port near the gun for the
ion pump, and an isolation valve for isolating the gun from the rest
of the vacuum system while the ion pump is working.
For LaB6 emitters, (1) you'll likely need modify your power supply
for the voltage/current requirements for heating the emitter, and (2)
you'll most likely need a different Wehnelt. (...hmmmmm? ... have I
forgot anything? ....)

cheerios, =shAf= :o)

{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {}
Michael Shaffer, R.A. - mshaf-at-darkwing.uoregon.edu
Geological Science's Electron Probe Facility - University of Oregon
http://epmalab.uoregon.edu/





From daemon Fri Sep 22 18:40:46 2000



From: BBDDHodges-at-aol.com
Date: Fri, 22 Sep 2000 19:31:00 EDT
Subject: ugly bug contest

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I am interested in finding out more about the Oklahoma City, OK Ugly Bug
contest which is sponsored by the Microscopy Society there. Thanks. Barbara
Hodges bbddhodges-at-aol.com



From daemon Sat Sep 23 00:24:36 2000



From: qoepe76-at-urumadelvi.co.jp (P.R.T.C.)
Date: Sat, 23 Sep 2000 13:12:19 +0800
Subject: Investigate Anyone

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From daemon Sat Sep 23 02:13:42 2000



From: =?ks_c_5601-1987?B?Sm9uZG8gWXVuIMCxwbi1tQ==?= :      jdyun-at-kyungnam.ac.kr
Date: Sat, 23 Sep 2000 16:02:17 +0900
Subject: Thanks for the dye-sub printer help

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Dear netters

With regard to my question on the model of dye sub printer for the EM photos, eighteen people have sent me replys. Most of them suggested to use Epson printer, 870, 900 or 1270 not only due to the price, but also due to the image quality. Dr. John Mackenzie also kindly recommended to use both of Epson 900 for the resolution and 870 for the archiving because of fading problem of 900. But some of them wrote that dye sub is better than ink jet printer. They were using Codonics, Fuji Pictography, Tektronics, or Kodak 8670 dye sub printers.

I am thinking to buy Epson 900 and small Kodak dye sub printer 4270. 4270 is not so expensive, and prints only 4 inch wide photos, good for EM photo only. Many of its users are for passport size personal photographs. I will see how good is resolution of 4270.

Epson 900 sprays 3 picoliters of color dots, smallest one that I've ever known, but 10 picoliters of grey-scale dots, somewhat large one. Epson 870 sprays 4 picoliters of color dots, but unknown for grey scale. I do not know how much difference 3 and 4 picoliters dots would make. Epson 870 is photo version and has various capability of photography. Epson 900 is for office color printing. If the difference is insignificant, 870 would be much better. Any comments?

Thank all of you who have kindly posted suggestions and comments.

Best regards,

Jondo Yun
Kyungnam University
Division of Advanced Materials
Electron Microscopy Laboratory
449 Weolyeong-dong
Masan, 631-701, Korea

(tel) 82-55-249-2697 (office)
82-55-249-2564 (EM lab)
82-55-249-2719 (Lab)
(fax)82-55-248-5033 (div. office)
(email) jdyun-at-hanma.kyungnam.ac.kr







From daemon Sat Sep 23 10:38:47 2000



From: Ken Converse :      qualityimages-at-netrax.net
Date: Sat, 23 Sep 2000 11:12:05 -0700
Subject: Re: Carbon tracking

Contents Retrieved from Microscopy Listserver Archives
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Gary M. Easton wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Listers, Anyone out there know of a good(hopefully easy!) way to
} remove carbon tracking deposits on a ceramic electon gun? Thanks in
} advance. Gary M. Easton Scanners Corporation
Gary,
The concentrated HCl is great but the metal parts become a real
problem. Try Bon Ami if the surface is glazed. It shouldn't scatch.
If it's not glazed then be more aggressive with Comet or Ajax.

Bon Ami also works wonders on a yellowed specimen chamber if you can
work with just the chamber in a sink. It beats acetone or alcohol
hands-down.

Ken Converse
Quality Images
Delta, PA




From daemon Sat Sep 23 12:01:10 2000



From: Caroline Schooley :      schooley-at-mcn.org
Date: Sat, 23 Sep 2000 09:48:45 -0700
Subject: Re: ugly bug contest

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}
} I am interested in finding out more about the Oklahoma City, OK Ugly Bug
} contest which is sponsored by the Microscopy Society there. Thanks. Barbara
} Hodges bbddhodges-at-aol.com

Barbara -

The Oklahoma Microscopy Society website is
http://www.ou.edu/research/electron/oms/, and the popular Ugly Bug contest
has its own (very entertaining) site:
http://www.ou.edu/research/electron/oms/uglybug/ Paige Johnson
{paige.l.johnson-at-iolok.com} chairs the OSM outreach
program.



Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html





From daemon Sun Sep 24 09:51:13 2000



From: Joseph Passero :      jp-at-spacelab.net
Date: Sun, 24 Sep 2000 10:33:17 -0400
Subject: LM Question about AO (American Optical) Eyepiecs

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Did AO (American Optical) ever make eyepieces with retical holder?

Thank You

Joseph Passero

mailto:jp-at-spacelab.net



________________________________________________________
1stUp.com - Free the Web
Get your free Internet access at http://www.1stUp.com



From daemon Sun Sep 24 15:55:48 2000



From: Guoda Lian :      glian0-at-engr.uky.edu
Date: Sun, 24 Sep 2000 16:41:20 -0400 (EDT)
Subject: Postdoctoral positions on Materials science

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Postdoctoral Positions in Materials Science
University of Kentucky


Two post-doctoral researcher positions are available at the
University of Kentucky, as detailed below. The research programs
will make extensive use of the university's Electron Microscopy
Facility, which houses two SEMs, two TEMs and one AFM. Most of the
research will be conducted on a JEOL 2010F Field Emission TEM
outfitted with an Oxford energy dispersive x-ray spectrometer (EDS),
Gatan electron energy loss imaging filter and acquisition hardware
and software for spectrum imaging.

1. The first position will be in the area of carbon-based materials
under the auspices of the NSF-sponsored Center for Advanced Carbon
Materials. The structure and chemistry of chemically-modified carbon
nanotubes and metal-doped diamond-like carbon films will be
investigated and correlated with physical property measurements.
Most experimentation will involve the use of high-resolution electron
microscopy (HREM) and electron energy loss spectroscopy (EELS).

2. The second position will be in the area of nanocrystalline oxide
materials for sensor applications. In collaboration with the
Chemistry Department at the University of Kentucky, we are studying
the crystallization kinetics of metal-oxide precursors which have
been designed at the molecular level to facilitate low-temperature
synthesis routes. Phase and particle analysis of various oxide
materials will be assessed quantitatively by TEM and XRD. Dopant
segregation and the effects on dielectric behavior will also be
studied.

Qualified candidates will have Ph.D. in Materials Science, Physics or
any related field and practical experience in analytical electron
microscopy. The salary will be commensurate with qualifications and
experience. Please send applications, including two references to:

Professor Elizabeth Dickey
Department of Chemical and Materials Engineering
University of Kentucky
A254 ASTeCC Bldg.
Lexington, KY 40506-0286
ecdickey-at-engr.uky.edu

The University of Kentucky is an equal opportunity employer.




From daemon Mon Sep 25 07:04:18 2000



From: STYLER-at-dnr.state.md.us
Date: Mon, 25 Sep 2000 07:51:36 -0400
Subject: LM PAS STAINING WITH AGAROSE

Contents Retrieved from Microscopy Listserver Archives
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Could you tell me if agarose is PAS positive? I stained a dinoflagellate
using agarose embedding technique and it was negative, however, others in my
field tell me it should have stained positive. Can you clarify?

Sue Tyler, Biologist
MD Dept. of Natural Resources
904 S. Morris Street
Oxford, MD 21654
410-226-5193






From daemon Mon Sep 25 08:13:41 2000



From: kepinski-at-int.pan.wroc.pl
Date: Mon, 25 Sep 2000 08:03:30 -0500
Subject: Edwards Auto 306 evaporator

Contents Retrieved from Microscopy Listserver Archives
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Hello,

Does anybody knows an efficient and quick way to get spare parts for
Edwards Auto 306 coater?.
It seems that EPROM in the control unit is dead and the instruments is out
of control (after switching on the display diodes light randomly). An
official Edwards' representative in Poland told us that it would take months
for them to get the needed parts from the Edwards headquarters.

Leszek Kepinski

Institute of Low Temperature and Structure Research,
Polish Academy of Sciences,
P.O.Box 1410,
50-950 Wroclaw, Poland

e.mail : kepinski-at-int.pan.wroc.pl,
http://www.int.pan.wroc.pl





From daemon Mon Sep 25 08:32:44 2000



From: =?iso-8859-2?B?TGVzemVrIEvqcGnxc2tp?= :      kepinski-at-int.pan.wroc.pl
Date: Mon, 25 Sep 2000 15:28:14 +0200
Subject: Edwards Auto 306 - help

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,

Does anybody know an efficient and quick way to get spare parts for
Edwards Auto 306 coater?.
It seems that EPROM in the control unit is dead and the instruments is out
of control (after switching on the display diodes light randomly). An
official Edwards' representative in Poland told us that it would take months
for them to get the needed parts from the Edwards headquarters.

Leszek Kepinski

Institute of Low Temperature and Structure Research,
Polish Academy of Sciences,
P.O.Box 1410,
50-950 Wroclaw, Poland

e.mail : kepinski-at-int.pan.wroc.pl,
http://www.int.pan.wroc.pl




From daemon Mon Sep 25 08:38:41 2000



From: Tobias Baskin :      BaskinT-at-missouri.edu
Date: Mon, 25 Sep 2000 08:30:14 -0500
Subject: Re: LM PAS STAINING WITH AGAROSE

Contents Retrieved from Microscopy Listserver Archives
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Greetings,
I have PAS stained samples embedded in agarose and then in
plastic and there was never an agarose reaction.

However, this info is simply anecdotal. I have no idea is PAS
is "supposed" to stain agarose, and I don't do PAS "properly" (just
perioidic acid and then Schiff's). Did you have a positive control?
Sometmes the Schiff's reagent goes bad. I recall placing a drop on a
piece of white tissue paper and if it went pink in a moment or two
then the reagent was still ok.

Hope this helps even a little,
Tobias

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

--
_ ____ __ ____ Tobias I. Baskin
/ \ / / \ / \ \ University of Missouri
/ | / / \ \ \ Biological Sciences
/_ / __ /__ \ \ \__ 109 Tucker Hall
/ / / \ \ \
Columbia, MO 65211-7400 USA
/ / / \ \ \ voice:
573-882-0173
/ /____ / \ \__/ \____ fax: 573-882-0123



From daemon Mon Sep 25 09:11:51 2000



From: Chuck Butterick :      cbutte-at-ameripol.com
Date: Mon, 25 Sep 2000 08:52:01 -0500
Subject: LN2 Safety Alert

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...passed on for the microscopy community


On Wednesday, September 20th, there was an accident at the New
York-Presbyterian Hospital that might be of some interest to members
of the list. It was to me since my wife works just outside the
accident area.

For those that get the New York Times, it was on page B12; it was on
page 2 of the New York Daily News.

In summary, a supervisor was killed by asphyxiation while working
alone in a poorly ventilated trailer located outside the hospital. He
was part of a team installing a new MRI at the hospital. For reasons
not clear, one of the liquid nitrogen tanks connected by a valve to
the room started leaking, filling the trailer space and causing the
asphyxiation. Six others were treated for dizziness and other minor
injuries. Two of the six injuries resulted from attempting to rescue
the supervisor as they went into the room before it had been
ventilated. At that time, it was not known that the room had been
filled with nitrogen and that the oxygen had been displaced.

I cannot determine if the trailer was a temporary facility or part of
the new installation that was being done. The nitrogen tanks were
outside the trailer and the trailer where the supervisor was working
was on the order of 15 x 40. It is not clear from the reports why the
supervisor was working alone or how long he had been working in the
trailer. Also, there is no indication of whether the trailer was part
of the installation or a temporary facility.

Just thought you all would be interested.

Tony Mitchell, Ph. D.
7 East Willow Street
Beacon, NY 12508




From daemon Mon Sep 25 10:39:38 2000



From: Roger Moretz :      rcmoretz-at-excite.com
Date: Mon, 25 Sep 2000 08:20:25 -0700 (PDT)
Subject: Re: SEM need help on endothelial monolayers

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Alan:

I have watched the replies to your question, and have noted that for the
most part they reflect my experience. Perhaps you could try to begin your
dehydration at very low %, e.g. 30% or even 10% EtOH, and do a more gradual
(10,20,30,40,50,60,70,80,90,90,100,100,100) series. This has worked for
some of the cultured cells I have prepared for SEM over the years. Other
little nuances have included a very slow bleed in for the initial CO2 step
in the CPD, and then watch the flow during the process. A final issue is
watching the temperature the CPD cycles at for final drying. Sometimes the
CPD tends to run a little hot, and that can also affect the final results.
Hope one or more of these are of use.

Roger Moretz, Ph.D.
Dept of Toxicology
Boehringer Ingelheim Pharmaceuticals
On Tue, 19 Sep 2000 14:16:25 -0500, Alan Burns wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
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} -----------------------------------------------------------------------.
}
}
} I am having trouble obtaining good SEM preparations of cultured
endothelial
} cell monolayers. The monolayers show evidence
} of artefactual cracking along cell borders. The cracks appear whether
the
} cells are grown on glass coverslips or transwell filters. I have tried
} various
} fixatives (buffered glutaraldehyde alone, glutaraldehyde followed by
osmium
} and uranyl acetate), different dehydrating agents (ethanol or acetone),
} followed by critical point drying (CPD). I have even tried
} tetramethylsilane (Ted Pella) in place of CPD. The only time I get good
} images (i.e.,
} no cracks) is when a small portion of the monolayer has inadvertently
} detached from its substrate during tissue processing. The cells on this
} small flap look marvelous (no cracks). I believe, in the absence of
} substrate adhesion, the cells on the flap shrink uniformly during
dehydration
} when surface tension forces exert their effects. Does anyone know how to
} prepare cell monolayers for SEM that leaves them intact and free
} from artefactual cracking?
}
} Thanks.
}
} Alan Burns, Ph.D.
} Assistant Professor
} Cardiovascular Sciences
} Department of Medicine
} Baylor College of Medicine
}





_______________________________________________________
Say Bye to Slow Internet!
http://www.home.com/xinbox/signup.html




From daemon Mon Sep 25 10:48:26 2000



From: Jill.Webb-at-rssl.co.uk
Date: Mon, 25 Sep 2000 17:15:24 +0100
Subject: Vacancy in RSSL's Microscopy Laboratory

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To: Dgambaro-at-eur.memc.com
cc:


RSSL's Microscopy Laboratory has an extremely varied and busy working
environment and its members of staff are committed to providing a first
class microscopy service. A major proportion of work concerns Foreign Body
Analysis, largely for the pharmaceutical and food sectors, for which the
Laboratory is a market leader and enjoys a good reputation amongst its
customers. Light and scanning electron microscopy imaging and Research and
Development accounts for most of the remainder of the work. In house
techniques available include LM (using different contrasting methods), SEM,
cryo-SEM, X-ray microanalysis, FT-IR microspectroscopy, image analysis and
freeze fracture/etching; TEM is undertaken off site.

Due to expansion and subsequent staff development, an opportunity has
arisen in to assist in the Foreign Body Identification service that runs
from the Microscopy Laboratory. The position also supports ongoing
research projects, particularly in both chocolate and sugar confectionery
project areas. Other tasks include provision of support in maintenance of
laboratory equipment including the electron and light microscopes, digital
imaging and dark room facilities. On occasions, work into the evenings and
at the weekends might be required and this post carries a requirement to
share in a rota to undertake out of hours analyses as part of RSSL's
comprehensive Emergency Response Service.

We are looking for a candidate who is educated to degree level or
equivalent with a minimum of 4 years microscopy experience in food science,
biology or materials science. Although training will be given, experience
in X-ray microanalysis of bulk samples and/or of FT-IR microspectroscopy is
highly desirable. Experience or knowledge of general equipment maintenance
and a willingness to become involved in this; be an excellent team player
with the ability to work independently; be customer focussed and able to
work under pressure, as well as capable of providing written reports of
long and short term projects.

If you are a self starter, looking for an interesting and challenging role
and believe you can make a positive contribution to our already successful
team, we would be interested to hear from you.

Please send your CV with a covering letter including your salary
expectations to Jane Bienkowski, Human Resources Officer, Reading
Scientific Services Lld, Lord Zuckerman Research Centre, PO Box 234,
Whiteknights, Reading, RG6 6LA or e:mail jane.a.bienkowski-at-rssl.co.uk or
fax to 0118 986 8932.




From daemon Mon Sep 25 11:57:20 2000



From: chris smith :      chris.smith-at-bbsrc.ac.uk
Date: Mon, 25 Sep 2000 17:44:31 +0100
Subject: Edwards E306A

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Hi Phil,
We have odd bitz for the above. Let me know the dimensions of the
feed thro and I'll have a rummage in the box. Chris Smith, IACR-Rothamsted,
UK.
chris.smith-at-bbsrc.ac.uk



From daemon Mon Sep 25 12:16:04 2000



From: Lena Falk :      lklfalk-at-fy.chalmers.se
Date: Mon, 25 Sep 2000 19:07:09 +0200
Subject: Course on SEM, Chalmers, Sweden

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Dear fellow microscopist,

Take the chance to learn about the developments in scanning electro
microscopy! A course on "Scanning Electron Microscopy - Imaging and
Microanalysis", SEM 2000, will be given at Chalmers University of
Technology, Gothenburg, Sweden, October 17 - 19, 2000.

This course will be given in collaboration with four microscope
manufacturers (Hitachi, Jeol, Leo, Philips/Fei) and suppliers of
equipment for EDX, WDX, EBSP and CL.

The aim of this 3-day intensive course is to give a theoretical
background in the morning sessions and experimental insights in the
afternoons. The lectures and the demonstrations will be given by
application specialists from the different companies representad at the
course, and also by researchers working with different applications in
SEM. The demonstrations and lab classes will be carried out on
equipment brought to Chalmers specifically for this course.

Detailed information about SEM 2000, including course programme and
registration form, is posted on: http://fy.chalmers.se/microscopy This
web-site will be continuously updated until the course starts.

Looking forward to seeing you at SEM 2000!

Birgitta Castedal, Lena Falk and Mats Halvarsson
Course Organisers

____________________________

Associate Professor Lena Falk
Department of Experimental Physics,
Chalmers University of Technology,
SE-412 96 Göteborg, SWEDEN

tel: +46 31 772 3321
fax: +46 31 772 3224
e-mail: lklfalk-at-fy.chalmers.se



From daemon Mon Sep 25 13:27:44 2000



From: Vitaly Feingold :      vitalylazar-at-worldnet.att.net
Date: Wednesday, September 20, 2000 1:34 PM
Subject: mu-metal

Contents Retrieved from Microscopy Listserver Archives
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Mark,

Your description does not fit the classic EM fields interference pattern, as
other responses had pointed out. The high level of the stray fields in the
room is bad, yet it will not necessary cause a problem. Your problem could
be resulted from a combination of conditions. JEOL, however, has a reason
for recommending the mu-metal shield.
You can perform a quick and easy test to see whether external
electromagnetic fields are responsible for the problem. Assuming that the
SEM column is clean (especially the final aperture!), and focusing circuit
operates properly.
1. Obtain an image in continuous LIVE (no integration!) mode on the monitor
screen with contrast enough vertical features, so distortion of the vertical
lines is clearly visible. Magnification may vary, but you will need at least
several 1000s. This distortion (if present), will be a sawtooth pattern,
with the period smaller at the slowest beam scan speed, larger at higher
speeds, and becoming a single "wave period" at the fastest speed which is a
TV rate. The later will be an image "floating" back and forth horizontally
on the screen. If the described pattern is not obtainable, the problem is
unlikely to be caused by stray AC fields.
2. Change accelerating voltage at least 50%. Focus the image. Make sure that
you have exactly the same sample feature in the image. Do not change any
other settings. If the described distortion is caused by external
electromagnetic fields, the amplitude of the sawtooth pattern (slow scan) or
the amplitude of "floating" (TV mode) will change in approximately reverse
proportion to the accelerating voltage value, i.e. at 10 kV will be twice of
what it was at 20 kV, and so forth.
3. If the distortion will stay the same, then the source of your problem
could be, for example, the mechanical vibrations. Those are caused by AC
electric motors (vacuum pumps, air compressors, elevators, etc.) most of the
time, vibration frequencies being harmonics of the AC line frequency. So the
distortion will look very similar, except its amplitude will not be a
function of accelerating voltage. Also, interference affecting the
electronic circuits (ground loops, for instance), may look the same and will
not be a function of the accelerating voltage.
4. Ground loops are the most frequent problem in my experience. Their
influence could be inconsistent, which makes the problem difficult to
identify. For example: the AC line conduit was used for the ground.
Everything was fine until big air-conditioning unit was installed behind the
wall. Now, a strong 60Hz AC field is measured every time when aircond.
motor kicks in. Then expensive mu-metal shield was installed, and... made
no difference.
The reason: new unit was connected to the same power line. Existing ground
loop was OK before due to low power consumption of the SEM. It is greatly
increased now, and ground loop badly affects electronics of the SEM. The SEM
column itself, at the same time, was sufficiently shielding external fields.
5. In any case, try self-made soft Iron shield before spending big $$ on it.

Good luck.

Vitaly Feingold
Scientific Instruments and Applications
2773 Heath Lane, Duluth GA 30096
(770)232-7785 ph.
(770)232-1791 fax
-----Original Message-----
} From: Mark West {mwest-at-ifcsun1.ifisiol.unam.mx}
To: microscopy-at-sparc5.microscopy.com {microscopy-at-sparc5.microscopy.com}




From daemon Mon Sep 25 15:17:18 2000



From: Kristen Lennon :      kalen-at-iastate.edu
Date: Mon, 25 Sep 2000 15:07:26 -0500
Subject: follow up to formaldehyde/formalin: safety

Contents Retrieved from Microscopy Listserver Archives
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Hi Again,
I received many replies to my question of late regarding the composition of
formalin vs. formaldehyde. All of them were pretty consistent except for
the most recent which touched on a very important, related issue, safety.
As I think that it is particularly important, I'm forwarding to the whole
list (with the permission of the author - who's name I've withheld). Be
careful out there.
Kristen

"Kristen,

I didn't follow the thread on this topic and what has been said. However,
the comment about being "pretty much covered with it while doing some mass
field fixes" caught my eye. In case no one else mentioned it, exposure to
formaldehyde is thought by some pulmonology specialists, especially those
researching Idiopathic Pulmonary Fibrosis (IPF), suspect that formaldehyde
exposure may trigger the disease. IPF is fatal and has no treatment except
lung transplant. I pass this on as one who has experienced the type of
exposure you describe, the diagnosis of IPF, and the double lung transplant
that is something that very, very few people are fortunate enough to
get. And even with a lung transplant, the five year life expectancy is
only 48%. PLEASE, please be very careful with lab chemicals and teach
those you work with to do likewise.

} My exposure to formalin/formaldehyde occurred during my undergraduate and
} graduate years, not only during standard class preparations but also in
} the field when we went collecting algae at freshwater and more so marine
} habitats. It was there when we were exposed to a lot of it; I remember my
} fingers getting "fixed", my eyes watering and stinging, you know the
} feeling. The fact is that the disease occurred about 20 years later. Yes
} the story has a "happy" ending, so far, but for reasons other than getting
} a rare double lung transplant and doing fine after 3 years. But that is
} another story! Yes, many people who handle chemicals in the lab don't get
} IPF, but don't take the attitude that nothing can happen to you. There
} are many other ways that chemical exposure can damage your
} health. Remember, take care of your body as it is the only one you get
} and damn few people get spare parts! :-)
}
} You may pass this along if you want, if it helps one person it's worth it."




From daemon Mon Sep 25 15:43:36 2000



From: mhtre123-at-yahoo.com
Date: Mon, 25 Sep 2000 15:07:26 -0500
Subject: follow up to formaldehyde/formalin: safety

Contents Retrieved from Microscopy Listserver Archives
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Thank you





From daemon Mon Sep 25 17:50:42 2000



From: John J. Bozzola :      bozzola-at-siu.edu
Date: Mon, 25 Sep 2000 17:40:30 -0500
Subject: GATAN DM2.1 problem

Contents Retrieved from Microscopy Listserver Archives
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I have a problem with Digital Micrograph 2.1 related to image file sizes.

Sometimes DM saves an image at 1.2MB while at other times (in fact,
the very next image) may be saved at 1MB or even less. Unfortunately,
when we do image analysis on these images the measurements will be
off since the number of pixels varies. I have tried saving in various
formats and nothing seems to change the events.

What gives here? Why does the program save in such differing file
sizes and so randomly? I can not figure out any logic to this.......
It is driving me crazzzzzzzzzzzzzzzyyyyyyy.

Thank you,

JB
####################################################################
John J. Bozzola, Ph.D., Director
Micro-Imaging and Analysis Center
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
####################################################################



From daemon Mon Sep 25 18:50:08 2000



From: Michael Plociniak :      plocinia-at-aecom.yu.edu
Date: Mon, 25 Sep 2000 19:37:49 -0400
Subject: Can LM predict immunogold TEM?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


A response directed to Paul Webster, c/o this invaluable and open forum:

I would like to continue with the topic of immunogold labeling for TEM,
relating to your suggestion:

"One possible step you could put in is to first try out your whole
preparation and immunolabeling protocol at the LM level. Use the same
reagents and same preparation protocols as you plan to use for EM and
expose to silver enhancment for a longer time so that you can see the
signal by LM. If you see a signal then you will be confident that you will
see a signal when you go to examine the EM experiment."

How reliable is LM for predicting TEM immunogold labeling results?

I have tried this approach before, with mixed results. It sounds like a
great idea, but in attempting to "modify" the EM protocol into a LM pilot
experiment, it becomes a completely different beast. During primary
fixation, EM requires glutaraldehyde to preserve ultrastructure. You
suggested that glut not be used until AFTER immunolabeling, but won't the
damage to the fine structure already be done? I was under the impression
that glut "postfixation" is intended primarily to crosslink gold antibodies
in place after they have bound to primary antibody.

Also, silver and gold enhancement reagents come in LM and EM formulae;
usually, EM versions are less acidic. Does a longer LM enhancement
accurately predict the action of a brief EM enhancement? Perhaps EM
formulae can be used to directly monitor enhancement with the light
microscope. However, because optimal EM enhancement is invisible by LM,
one will need to empirically determine EM enhancement times for each cell
system. Doesn't this argue against spending too much time with LM
experiments, when EM also includes osmium, uranyl acetate, dehydration, and
resin infiltration?

I still use pilot LM experiments, but more for their complimentary benefit,
rather than for their predictive value.

Routinely, I see a discouraging signal to noise ratio at the LM level.
However, at the EM level noise is restricted to the first couple of resin
sections of a monolayer culture, and subsequent sections contain beautiful
results. I interpret this as nonspecific binding of antibodies to the
poly-L-lysine and lamin-coated glass substrate. In this example, routine
LM (DIC optics without optical sectioning) does not accurately predict EM
performance.

Sincerely,

Michael Plociniak
Research Technician
Albert Einstein College of Medicine
Neuroscience Dept.
Rose Kennedy Center, 529
Bronx, NY 10461
(718) 430-3509
plocinia-at-aecom.yu.edu

transmission and scanning electron microscopy




From daemon Mon Sep 25 19:33:32 2000



From: Karen Pawlowski :      Karen.Pawlowski-at-worldnet.att.net
Date: Mon, 25 Sep 2000 19:25:16 -0500
Subject: Thankyou DFW

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thanks everyone who responded to my request for labs in the DFW area
that would
consider "renting" space on their scopes. I've sent the information on
to the researcher
who is looking for space and he and I were impressed by the many places
that were
gracious enough to consider letting outsiders use their equipment.

Thanks again,

Karen Pawlowski, PhD






From daemon Tue Sep 26 08:02:11 2000



From: Gareth Morgan :      Gareth.Morgan-at-impi.ki.se
Date: Tue, 26 Sep 2000 08:20:26 +0200
Subject: Re: LM PAS STAINING WITH AGAROSE

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Tobias

Whenever I use Schiffs reagents for student lab classes I test it first by
adding just a couple of drops of neutral buffered formalin (the diluted
form = 3.8% formaldehyde) to about 10ml in a clear tube and they can then
watch the colour develop over a few seconds. If was also 'water white' to
begin with and with no hint of pink we use it. It hasn't failed me so far
and the students get to see a dramatic colour change before they do the
method.

Hope this helps?



At 08:30 2000-09-25 -0500, Tobias Baskin wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Med vänliga hälsningar/With best regards

Gareth

"A wise person is certain of few things - a fool of everything"

"The foolish reject what they see, not what they think;
the wise reject what they think, not what they see." Huang Po

Gareth Morgan MPhil MSc FIBMS,
Institutionen för Mikrobiologi, Patologi och Immunologi(IMPI), H5,
Karolinska Institutet,
Avdelningar för biomedicinsk
laboratorievetenskap och
biomedicinska ämnen,
Box 12 773,
S 11 296, Stockholm
Sverige

NB/Obs! Visiting address =

Lindhagensgatan 92
Kungsholmen

e-mail Gareth.Morgan-at-impi.ki.se
Tel +46 8 728 3734
Fax +46 8 728 3688



From daemon Tue Sep 26 08:21:07 2000



From: MHHulbert-at-aol.com
Date: Mon, 25 Sep 2000 22:34:44 EDT
Subject: TEM Symposium Announcement & Call for Papers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



NRL Symposium, “TEM at the Frontierâ€

In celebration of the acquisition of a new JEOL 3010 transmission
electron microscope with environmental cell capabilities, the Naval
Research Laboratory Stennis Space Center will be conducting a day long
symposium on November 2, 2000. Outstanding microscopists from around
the country will join local experts to discuss marine environmental and
materials science applications of TEM. On Friday morning, November 3,
participants are invited to visit informally with NRL researchers in
the new Microscopy Facility.

In addition to the opportunities for exploring and sharing insights
during the period of formal presentations, transportation will be
provided Thursday evening to the French Quarter in New Orleans. Here
participants may enjoy interacting with each other in a setting famous
for its music, its food, and its stimulating atmosphere.

We are also strongly soliciting contributed papers. Although a primary
area of emphasis is expected to be environmental cell and in-situ
microscopy, submissions of a wide variety of ‘frontier’ microscopy
results are welcome!


Invited speakers include:
Larry Allard - Oak Ridge National Laboratory -- “Aberration
Correction for High Resolution Imaging and Advanced Analytical
Electron Microscopyâ€
Frances Ross - IBM Watson Research Center
Renu Sharma - Arizona State University
Judith Yang - University of Pittsburgh

Other speakers planning to participate include:
Tyrone Daulton – NRL -- “Application of High-Resolution and
In-Situ TEM to Solving Problems in Diverse Fields of Studyâ€
Yoko Furukawa – NRL -- “Ultrastructure of Aquatic Sediments and its
Effect on Early Diagenesisâ€
Dawn Lavoie – NRL -- “Preparation Techniques and Quantitative
Characterization of Clay Sediments using TEMâ€
Brenda Little – NRL -- “Environmental Electron Microscopy
Investigations of Microbiologically Influenced Corrosionâ€
Eric Stach – NCEM -- “In-situ TEM of Dislocations in Thin Filmsâ€

This symposium is co-sponsored by the Southeastern Microscopy Society.

We anticipate there will be approximately 16 presentations at the
Symposium. Arrangements are underway to develop a special section of
Microscopy and Microanalysis devoted to the papers presented. Finished
manuscripts are due October 15. The total number of participants will
be limited to a maximum of 60.

REGISTRATION
Please register early by completing and returning the form
below; the registration deadline is October 13.


Sincerely, your organizers:
Bhakta Rath, Co-Chair - Naval Research Laboratory
Eric Stach, Co-Chair - National Center for Electron Microscopy,
Lawrence Berkeley National Laboratory
Matthew Hulbert, Coordinator - Research Dynamics


REGISTRATION INFORMATION

Name: ______________________________________________________

Organization: ______________________________________________

Address: ___________________________________________________

___________________________________________________________

e-mail Address: ____________________________________________

______I wish transportation to the French Quarter Thursday evening.

______I do not wish transportation to the French Quarter.


If you wish to give a presentation, please provide its title below.



Return registration information to Matthew Hulbert.
E-mail is preferred -- matthew_hulbert-at-hotmail.com
If you choose to use US mail, the address is
1332 Pembroke Drive, West Chester, PA 19380.



From daemon Tue Sep 26 08:36:29 2000



From: Pam Marcum :      pmarcum-at-polysciences.com
Date: Tue, 26 Sep 2000 09:25:55 -0400
Subject: Re: LM PAS STAINING WITH AGAROSE

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Most standard histology books will give a procedure for digestion of a
sample as negative control. If you need the procedure please let me know
and I will e-mail it to you.

Pamela A Marcum
Histology/Microscopy
Product Development Manager
Polysciences, Inc.

-----Original Message-----
} From: Tobias Baskin [mailto:BaskinT-at-missouri.edu]
Sent: Monday, September 25, 2000 9:30 AM
To: Microscopy-at-sparc5.microscopy.com
Cc: STYLER-at-dnr.state.md.us


Greetings,
I have PAS stained samples embedded in agarose and then in
plastic and there was never an agarose reaction.

However, this info is simply anecdotal. I have no idea is PAS
is "supposed" to stain agarose, and I don't do PAS "properly" (just
perioidic acid and then Schiff's). Did you have a positive control?
Sometmes the Schiff's reagent goes bad. I recall placing a drop on a
piece of white tissue paper and if it went pink in a moment or two
then the reagent was still ok.

Hope this helps even a little,
Tobias

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

--
_ ____ __ ____ Tobias I. Baskin
/ \ / / \ / \ \ University of
Missouri
/ | / / \ \ \ Biological
Sciences
/_ / __ /__ \ \ \__ 109 Tucker Hall
/ / / \ \ \
Columbia, MO 65211-7400 USA
/ / / \ \ \ voice:
573-882-0173
/ /____ / \ \__/ \____ fax: 573-882-0123




From daemon Tue Sep 26 08:46:59 2000



From: Matthew Hulbert :      matthew_hulbert-at-hotmail.com
Date: Tue, 26 Sep 2000 08:36:49 -0500
Subject: NRL Symposium, TEM at the Frontier

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



NRL Symposium, ìTEM at the Frontierî

In celebration of the acquisition of a new JEOL 3010 transmission
electron microscope with environmental cell capabilities, the Naval
Research Laboratory Stennis Space Center will be conducting a day long
symposium on November 2, 2000. Outstanding microscopists from around
the country will join local experts to discuss marine environmental and
materials science applications of TEM. On Friday morning, November 3,
participants are invited to visit informally with NRL researchers in
the new Microscopy Facility.

In addition to the opportunities for exploring and sharing insights
during the period of formal presentations, transportation will be
provided Thursday evening to the French Quarter in New Orleans. Here
participants may enjoy interacting with each other in a setting famous
for its music, its food, and its stimulating atmosphere.

We are also strongly soliciting contributed papers. Although a primary
area of emphasis is expected to be environmental cell and in-situ
microscopy, submissions of a wide variety of ëfrontierí microscopy
results are welcome!

EARLY REGISTRATION WOULD BE GREATLY APPRECIATED!!!

Invited speakers include:
Larry Allard - Oak Ridge National Laboratory -- ìAberration
Correction for High Resolution Imaging and Advanced Analytical
Electron Microscopyî Frances Ross - IBM Watson Research Center
Renu Sharma - Arizona State University
Judith Yang - University of Pittsburgh
Other speakers planning to participate include:
Tyrone Daulton ñ NRL -- ìApplication of High-Resolution and
In-Situ TEM to Solving Problems in Diverse Fields of Studyî
Yoko Furukawa ñ NRL -- ìUltrastructure of Aquatic Sediments and its
Effect on Early Diagenesisî
Dawn Lavoie ñ NRL -- ìPreparation Techniques and Quantitative
Characterization of Clay Sediments using TEMî
Brenda Little ñ NRL -- ìEnvironmental Electron Microscopy
Investigations of Microbiologically Influenced Corrosionî
Eric Stach ñ NCEM -- ìIn-situ TEM of Dislocations in Thin Filmsî

This symposium is co-sponsored by the Southeastern Microscopy Society.

We anticipate there will be approximately 16 presentations at the
Symposium. Arrangements are underway to develop a special section of
Microscopy and Microanalysis devoted to the papers presented. Finished
manuscripts are due October 15. The total number of participants will
be limited to a maximum of 60.

REGISTRATION
Please register early by completing and returning the form
below; the registration deadline is October 13.

Sincerely, your organizers:
Bhakta Rath, Co-Chair - Naval Research Laboratory
Eric Stach, Co-Chair - National Center for Electron Microscopy,
Lawrence Berkeley National Laboratory
Matthew Hulbert, Coordinator - Research Dynamics

REGISTRATION INFORMATION
Name: ______________________________________________________
Organization: ______________________________________________
Address: ___________________________________________________

___________________________________________________________
e-mail Address: ____________________________________________
__I wish transportation to the French Quarter Thursday evening.
__I do not wish transportation to the French Quarter.
If you wish to give a presentation, please provide its title below.
Return registration information to Matthew Hulbert.
E-mail is preferred -- matthew_hulbert-at-hotmail.com
If you choose to use US mail, the address is
1332 Pembroke Drive, West Chester, PA 19380.
_________________________________________________________________________
Get Your Private, Free E-mail from MSN Hotmail at http://www.hotmail.com.

Share information about yourself, create your own public profile at
http://profiles.msn.com.





From daemon Tue Sep 26 09:18:47 2000



From: Alan W. Nicholls :      nicholls-at-uic.edu
Date: Tue, 26 Sep 2000 09:03:51 -0500
Subject: TEM CCD camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers

Have any other microscopists had problems with Peltier coolers failing on
water cooled CCD cameras?

In my facility I have three such cameras all plumbed into the microscope
water system and connected, by the camera manufacturer, into wall sockets.
On one of the three cameras I have had the Peltier cooler fail four times
in two years. After the latest failure the manufacturer asked if the
camera had been run without water. I told him not intentionally but when
we have power cuts and the power is restored, the camera comes back on but
the microscope (and water) does not. The camera can therefore run for some
time without water cooling. Power failures are not uncommon at this
university!

Many e-mails to the manufacturer have resulted in no response to my
questions about reliability of the Peltier coolers. The manufacturer is
treating the failure as an out of warranty issue but I believe there has
been something wrong with this camera since it was installed.

Any experiences of listservers with Peltier cooler failures would be
appreciated. Please e-mail me directly.

Regards

Alan W Nicholls, PhD
Electron Microscopy Service Director
Research Resources Center - East (M/C 337)
Room 100 Science and Engineering South Building
The University of Illinois at Chicago
845 West Taylor St
Chicago, IL 60607-7058

Tel: 312 996 1227
Fax: 312 996 8091
Office: Room 110

Web site www.rrc.uic.edu


Alan W Nicholls, PhD
Electron Microscopy Service Director
Research Resources Center - East (M/C 337)
Room 100 Science and Engineering South Building
The University of Illinois at Chicago
845 West Taylor St
Chicago, IL 60607-7058

Tel: 312 996 1227
Fax: 312 996 8091
Office: Room 110

Web site www.rrc.uic.edu



From daemon Tue Sep 26 09:59:33 2000



From: Larry Allard :      l2a-at-ornl.gov
Date: Sun, 26 Sep 2004 22:52:38 -0400
Subject: Re: TEM CCD camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Alan:

Just this morning we were discussing the same problem with our Gatan
CCD cameras on our HD-2000, one of which is retractable and used for
TEM imaging, and the other of which is at the end of the GIF for
energy-filtered imaging. These cameras are connected in series for
the water flow, and have very tiny water lines which can easily clog,
even with recirculated water. There is no provision by the
manufacturer to provide for a safety cut-off of the Peltier cooler in
the event of loss of water flow. We have recently experienced a
burn-out of the TEM camera due to a clog in the line, which resulted
in 2 weeks of downtime for repair (nicely done for free by Gatan),
but still no response to queries about some sort of protection. So
we have decided to find an appropriate water flow sensor, and to
install it in an appropriate place in the water line, and to connect
it to the camera controllers to provide a power cut-off when the
water flow diminishes. I'll post the details of the solution when it
is completed.

Larry





} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Dr. Lawrence F. Allard
Senior Research Staff Member
High Temperature Materials Laboratory
Oak Ridge National Laboratory
1 Bethel Valley Road
Bldg. 4515, MS 6064
PO Box 2008
Oak Ridge, TN 37831-6064

865-574-4981
865-576-5413 Fax
allardlfjr-at-ornl.gov



From daemon Tue Sep 26 10:34:36 2000



From: sghoshro-at-nmsu.edu
Date: Tue, 26 Sep 2000 09:21:58 -0600 (MDT)
Subject: TEM/SEM bacteria protocol

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I am having trouble processing bacteria in liquid culture for TEM/SEM.
Too much of sample is being lost because I only have a small table top
microfuge. The culture won't spin down into a hard enough pellet. Does
anyone have a different protocol for processing bacteria in liquid
culture? Please reply to capri-at-zianet.com
Kristine Fambrough







From daemon Tue Sep 26 12:14:55 2000



From: William F. Tivol :      wft03-at-health.state.ny.us
Date: Tue, 26 Sep 2000 13:00:26 -0400
Subject: Re: TEM CCD camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Dear Alan,
My experience with Peltiers does not involve a CCD camera, but it may be
instructive nonetheless. When we wanted to provide a constant temperature for
the chemicals in the darkroom--which required sometimes heating the water and at
other times cooling it--we decided that Peltiers were the best solution. We
bought a commercial thermoregulating unit which sent current of the appropriate
polarity through the Peltiers to adjust the temperature to the desired setpoint.
The Peltiers failed at an expensive rate, and we found that the thermoregulator
was such that it provided a specific current, which it turned on and off in a
duty cycle (the greater the temperature difference, the greater fraction of time
the current was on). The sudden changes in current were the cause of the
Peltiers' demise. Our shop designed and built a regulator which operated in
such a way that the current was not turned on and off, but was proportional to
the temperature difference, and the Peltiers worked properly for many years. We
were also warned that moisture in the Peltiers could lead to an electrochemical
process which caused corrosion and subsequent failure. Check that the
controller does not vary the current rapidly and that the Peltiers are protected
from condensation in your CCD setup.

Bill Tivol
Wadsworth Center
Albany NY
(518) 473-7399 WFT02-at-health.state.ny.us





From daemon Tue Sep 26 12:14:55 2000



From: Voith, Adam L :      avoith-at-fhcrc.org
Date: Tue, 26 Sep 2000 10:04:19 -0700
Subject: Seattle Announcement - Electron Micorscopy Specialist Search - Fr

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



PLEASE DON'T RESPOND TO SENDERS ADDRESS. RESPOND TO: jobresponses-at-fhcrc.org




FRED HUTCHINSON CANCER RESEARCH CENTER
ELECTRON MICROSCOPY SPECIALIST - (#SD-10483)

Perform all technical operations necessary to produce specimens for viewing
in the scanning and transmission electron microscopes. Will work
independently and report to the EM Manager in performing daily
responsibilities according to established procedures. Focus of the job is on
the application of skills and techniques to assist users with the extremely
varied research projects. Duties include preparation of specimens for TEM
and SEM, autoradiography, immunoelectronmicroscopy, serial sectioning,
shadowing, negative stating, particle counting and SEM. Produce prints and
publication ready layout. Periodic instrument maintenance, billing
operations, maintain logs, supply inventories, purchase records. Attend
workshops and maintain current knowledge of topics related to EM and Center
experimentation.

QUALIFICATIONS: BS/BA in biological science required. (MS a plus), minimum
of five years experience in cutting thin sections form a variety of tissues
required, speed, dexterity and expertise in cutting are critical to success
in this high production laboratory, ability to work accurately and
independently, strong computer skills, strong interpersonal and the ability
to deal with a variety of people required, ability to provide customer
service for a support facility required, must be a team player, strong
attention to detail and an eye for presentation required.

CLOSING DATE: Until filled

MAIL / EMAIL / FAX RESUME AND COVER LETTER OR SUBMIT IN PERSON TO: Human
Resources Office # 10483 / Fred Hutchinson Cancer Research Center / 1300
Valley Street / Seattle, WA 98109 / Fax: (206) 667-4051 / Email:
jobresponses-at-fhcrc.org / TTY: (206) 667-6861 - for deaf and hard of hearing
callers. An Equal Opportunity Employer Committed to Work Force Diversity.






From daemon Tue Sep 26 12:26:43 2000



From: Dohnalkova, Alice :      Alice.Dohnalkova-at-pnl.gov
Date: Tue, 26 Sep 2000 10:18:40 -0700
Subject: GATAN DM2.1 problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


John,
for all high resolution work and image analysis, I always collect images as a
".gat" (with a Digital Micrograph 3.3.1 on our system, I get 2.1 MB file). I
have similar problem with exporting files to users not having DM (not able to
open gatan files); either tiff or jpeg files are only about half the original
size. I get better resolution if I go through the "File/Export" option rather
than just "File/Save As/". Does anyone have a more elegant way? Thanks, Alice.

Alice Dohnalkova
Environmental Microbiology
Battelle, PNNL
MS P7-50
Richland, WA 99352
(509) 372-0692








} From: John J. Bozzola [mailto:bozzola-at-siu.edu]
Sent: Monday, September 25, 2000 3:40 PM
To: microscopy-at-sparc5.microscopy.com


I have a problem with Digital Micrograph 2.1 related to image file sizes.

Sometimes DM saves an image at 1.2MB while at other times (in fact,
the very next image) may be saved at 1MB or even less. Unfortunately,
when we do image analysis on these images the measurements will be
off since the number of pixels varies. I have tried saving in various
formats and nothing seems to change the events.

What gives here? Why does the program save in such differing file
sizes and so randomly? I can not figure out any logic to this.......
It is driving me crazzzzzzzzzzzzzzzyyyyyyy.

Thank you,

JB
####################################################################
John J. Bozzola, Ph.D., Director
Micro-Imaging and Analysis Center
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
####################################################################



From daemon Tue Sep 26 12:38:40 2000



From: Jan Leunissen :      leunissen-at-aurion.nl
Date: Tue, 26 Sep 2000 19:42:30 +0200
Subject: Immuno LM/EM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Kristine
There was a thread on this a few months ago, the concensus of
which was that for TEM most people advocated solidifying a spun-
down bacterial suspension using agar or agarose before getting too
far into the process. You might want to consider whether this should
be done pre-fix or post-fix. I would advocate the latter. Anyway, this
produces a chunk of material that can be handled through teh
solution changes more conveniently than a cell suspension.
However, many people found that a pellet solidifed in this way would
infiltrate rather poorly if left in the bottom of the microfuge tube /
eppendorff, etc. The best solution is probably to detach it and
perhaps chop it up a little to minimize specimen size, providing
reagents and solvents free access to all surfaces.
Best wishes
Chris Jeffree

} From: "sghoshro-at-nmsu.edu"-at-sparc5.microscopy.com
Date sent: Tue, 26 Sep 2000 09:21:58 -0600 (MDT)
To: microscopy-at-sparc5.microscopy.com


The original question by Tom Phillips has stirred up a great discussion
on this list. Even though we're getting a little away from the original
subject, I wanted to throw in my views with respect to the issue of
antibodies working in LM and not in EM. I'm afraid it will get kind of
lengthy, but I hope it is useful.

When immunodetection at the LM level works, but not at the EM level, I
prefer to follow a logical procedure to find where the bottle necks are.
Basically there may be two differences in the approaches:
1. the reagents used,
2. the specimen preparation method.

To make things simple, let us assume the labeling at the LM level works
reliably. Then we are left with a situation where we need to know
whether the antigens (epitopes) are still recognizable and available in
the EM specimen and whether the reagents for EM detection will do the
job they are supposed to do. How do we get an answer to these questions?

The first thing one should do is to check the reactivity of the reagents
for EM with the primary antibody of interest. This does not necessarily
have to be checked on the specimen. It is even better not to do that,
because there are too many other parameters different from the LM
approach. A simple dot-spot test can do the trick and give a conclusive
answer in about two hours. In practice: a dilution series of primary
antibody (monoclonal or affinity purified) is applied to a strip of
nitro-cellulose. The strip is blocked and incubated with the immunogold
reagent. If applicable (with small particle conjugates) the strip can be
silver enhanced.
If antigen is available, the dot-spot test can even be used to get a
rough idea about the influence of fixatives. A dilution series of
antigen (or an enriched cell lysate) is applied to the nitro-cellulose
strip, the strip can be treated with fixative (exactly the same
concentrations, time and temperature as intended to be used for specimen
fixation), then the strip is washed with glycine, blocked, incubated
with primary antibody, gold conjugate and silver enhanced. A comparison
of different strips (fixed with several different fixatives and unfixed)
gives somewhat of an indication whether a particular fixative is
worthwhile to try. It is not an absolute test, as the environment of the
antigen may play a role in the effect of fixation as well
(cross-linking, densely packed structures). Should anyone need
information how to do this in practice, please get in touch with us.
If antigen is not available, you could use cryostat sections or even
paraffin sections that worked at the LM level and run the same test.
Make sure that the gold and silver enhancement reagents being tested
here are the same reagents that will be used for EM labeling.

What about the influence of specimen preparation? To some extent the
issue of fixation has been addressed above. If reactivity of the
immunogold and silver enhancement reagents had been tested fine and EM
specimens had been prepared the same way as for LM (only thinner of
course, at least in the end), the approach should work. Unfortunately,
the specimen preparation for EM often differs a lot from the one for LM.
So let us start with the EM sample preparations that are similar to
those for LM, because logically those should be the most suited, at
least from the point of view of antigen preservation and presentation.
In ultrathin cryosections the influence of the embedding material is
avoided, so antigens will not be as masked as they are in resin or
acrylate embedded specimens. I don't know if there still is the same
consensus about the validity of this technique as there was some years
ago, but we always found that cryo ultra microtomy was the approach to
most likely give a positive result.

Another approach, that has been addressed in a few postings recently, is
to use pre-embedding labeling. In the time period before ultra small
gold conjugates became available, pre-embedding immunolabeling with the
classic gold conjugates was only feasible after harsh detergent
treatments which were often applied during chemical fixation. As a
result much of the cytoplasm and organelles were either destroyed or
washed out.
The state of ultrastructural preservation was far from ideal and this
has imprinted the pre-embedding approach with a negative image for a
long time. However, with the ultra small gold conjugates becoming
available, harsh detergent treatments of the sample as a trade off for
immunoreagents to penetrate were no longer necessary. Even only a
pre-treatment with sodium borohydride, which was applied to quench
aldehyde groups, was sufficient to make some types of single cells
accessible for ultra small gold reagents.

I don't want to state that these two approaches would be the only ones I
would consider useful. But they are good steps to start with. A great
advantage of the combination of ultrathin cryosections and ultra small
gold reagents is that these reagents penetrate with greater ease into
the section’s interior, compared to larger gold conjugates, detecting
not just antigens exposed on the surface.

For me cryosectioning also worked with plant material like soy beans,
but then again they were not the dormant dry seeds, these were sprouts.
I am sure they are a lot easier to cut than the dry ones. Teaching
workshops at botany departments I have learned how hard it is to get
even an acceptable level of ultrastructural preservation and good
immunolabeling, although I got the impression that high pressure
freezing can be a very good starting point.

To be able to use ultra thin cryosections you need a cryo ultra
microtome. For pre-embedding you don't need a lot of fancy equipment.
The results some of us are getting with pre-embedding immunolabeling are
really very encouraging, especially with Fab2 and single Fab
fragment-ultra small gold reagents and efficient and homogeneous silver
enhancement reagents being available. I don’t think I should be the one
doing a lot of talking here since it is not my work. But I hope that
researchers who have done this or are experienced with pre-embedding
will jump into the discussion. Hong, why don't you tell us?
And yes, I realize, useless for internal antigens in plant tissue. But
for cultured cells and animal tissue it can be great.

I wholeheartedly agree with Paul Webster that it is a must for an
investigator to try to understand the ins and outs of a method and that
‘we must stop being the "black box" of science’. That is the only way to
understand the results and to make progress.
When I was still associated with the University in Utrecht I experienced
very often that non-EM people have a tendency to look upon EM as a way
to make the picture to fit the story. As a sort of confirmation of the
biochemical or molecular data they already have fixed in their minds.
You're lucky (!) if you can confirm their beliefs, and they're happy to
have a nice demonstration supporting their ideas. IEM is a scientific
method that not just adds up to the results that are there, but my own
experiences convinced me that it may provide results that have been
overlooked with other, biochemical or whatever analysis methods. I
learned something from my PhD professor that I still value very much:
"don't try to get the results that you or somebody else wants you to
get, but try to understand the results you're actually getting".

In spite of the fact that most of you will have fallen asleep by now as
a result of this lengthy posting, I would like to make a few more
comments.

Don't compare apples with pears! Use the same reagents for LM and EM.
Protocols for LM and EM should be comparable as much as possible,
including the use of buffers, blocking reagents, dilutions.

I am reluctant about using cell fractionation in some instances: you
might only get to see what you want to see. This may degrade the
labeling to a confirmation of a mental picture, and prevent you to find
labeling in places you didn't expect before and which may not be present
in your fraction-specimen anymore. And also, if you already know where
the antigen is, why would you bother to do immuno EM? But often this
so-called knowing is just an idea based on data acquired up to that
point in time. Immuno EM may reveal additional data that shed a new
light on the question.

Secondary antibody conjugates vs. protein A: theoretically secondary
antibodies give less resolution, true. Is there anyone who actually made
the comparison and found one to be different from the other in that
respect? I mean not in molecular labeling: molecules or viruses attached
to a film on grid and labeled for specific epitopes, but labeling in or
on a section?

To end this message: unfortunately, sometimes it happens that even if
you've checked carefully and think it should work, sometimes it doesn't.
Those cases are rare and although they are most annoying they are also
the most interesting cases from a technological/methodological point of
view, because they can teach us something we didn't understand before.


Jan





From daemon Tue Sep 26 12:53:42 2000



From: Norm Olson :      nho-at-bilbo.bio.purdue.edu
Date: Tue, 26 Sep 00 12:46:27 -0500
Subject: GATAN DM2.1 problem

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} From: John J. Bozzola, bozzola-at-siu.edu

{I have a problem with Digital Micrograph 2.1 related to image file sizes.

{Sometimes DM saves an image at 1.2MB while at other times (in fact,
{the very next image) may be saved at 1MB or even less. Unfortunately,
{when we do image analysis on these images the measurements will be
{off since the number of pixels varies. I have tried saving in various
{formats and nothing seems to change the events.

{What gives here? Why does the program save in such differing file
{sizes and so randomly? I can not figure out any logic to this.......

Fun with Digital Micrograph :-(

Actually in DM2.X and lower when you "Save As.." you are in effect saving
a screen resolution image and the size of the image you save is dependent
on how much you have zoomed the image on the screen. So if you zoomed
out once and the image was 512X512 on screen (with a 1024 camera) then
your image would get saved as a 512X512 image and you lost half of your
resolution. What I always did was to save the image in DM format first
without touching anything in the image (contrast, brightness, zoom, etc).
That way I knew I had a pristine image that had not been messed up in
anyway and I could go back to that when needed. In order to save a tiff
or whatever, I would make sure that my zoom was 1:1 and then I would save
it in the format needed.

In DM3.X they modified that slightly. If you hit "Save as.." and save it
in DM format it is OK, if you "Save as.." in any other format it saves
just as I mentioned above. However, they now have an export menu item
that saves the image in original resolution in whatever file type you
want. I always tell my students to ignore the "Save as.." feature and
just use export. Gatan has also removed some of the bugs in the auto
save feature. If you have the extra $$$$$$$$$ it is probably worth
upgrading to DM3.X just note that they changed their DM image format
somewhat but new DM can read old DM images but not the other way around.

Norm Olson

*******************************************
Norm Olson
Senior Research Electron Microscopist
Department of Biological Sciences
Lilly Hall of Life Sciences
Purdue University
West Lafayette, IN 47907

Phone: 765-494-5643
FAX: 765-496-1189
email: nho-at-bragg.bio.purdue.edu
http://bilbo.bio.purdue.edu/~nho/index.htm
http://bilbo.bio.purdue.edu/~baker/

*******************************************




From daemon Tue Sep 26 15:16:19 2000



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Wed, 27 Sep 2000 08:11:57 GMT+1200
Subject: Power failures

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Hi, Alan


} we have power cuts and the power is restored, the camera comes back on but
} the microscope (and water) does not. The camera can therefore run for some
} time without water cooling. Power failures are not uncommon at this
} university!
}

I think that all equipment like this should be set up so that when
the power goes off, the equipment stays off when the power comes on
again.

Devices to acheive this are common in the world of electricians, they
call them "zero-voltage switches" (at least down here they do).

Get your electrician to install one for the mains socket from which
the camera derives its power.

Mine have saved me from complications many times.

cheers

rtch


Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand



From daemon Tue Sep 26 15:23:18 2000



From: Paul Webster :      pwebster-at-mailhouse.hei.org
Date: 26 Sep 00 13:22:41 -0700
Subject: RE: Can LM predict immunogold TEM?

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From: Paul Webster :      pwebster-at-mailhouse.hei.org
Date: 26 Sep 00 13:22:41 -0700
Subject: RE: Can LM predict immunogold TEM?

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Reply to: RE: Can LM predict immunogold TEM?
Hi Michael,

I read your comments with some dismay. I may have misunderstood, but the impression I get is that you feel there will be such large differences between LM labeling and EM labeling that you don't think it worthwhile to check your antibodies by LM first. If there were such differences then the experiment should not be performed in the first place. The LM result should be the same as the one you see by EM. I would not expect anything less.

One point of my previous posting was to suggest that immunolabeling experiments can be carefully designed so that LM screening is possible before moving onto the EM level. I think that the reason my antibodies work for EM is that I first tested them by LM to make sure I would get a result.

I do not think that glutaraldehyde is a prerequisite for good morphology, or that samples have to be treated with osmium tetroxide, uranyl acetate either. What I was suggesting is that it should be possible to screen samples by LM before moving onto the EM level. This will confirm that immunoreagents will react with the sample, will give an idea of the best dilutions to use, and will give information on the relative number and ditribution of antigens. All this should be possible using the same samples for LM and EM.

As an example, suppose I wanted to look for a mast cell antigen in nasal mucosa. I know from my biology lessons that there will be few mast cells in this tissue so I know I will have to search for them before I can label them. Now I make a choice between pre- and post-embedding labeling. I know that the antigen I want is going to be inside the granules of the mast cells, which are inside the tissue. For this reason, I decide that pre-embedding labeling may not be the best approach. I choose to label sections. Now, I have to decide whether I want to cryosection or to embed in resin. As I am lucky enough to have a cryoultramicrotome in my lab, and as I know from experience that I usually get higher sensitivity on cryosections, I choose to prepare the samples this way.
If I want to perform immunolabeling on the samples, I will choose not to fix in glutaradehyde, which gives the tissues an autofluorescent glow (and which can also be a useful counterstain, when used properly). I therefore choose to fix with formaldehyde (which, btw, is a gas and which when dissolved in water becomes formalin, and is actually converted to methylene glycol CH2O + H2O = HO CH2 OH). I also know that at concentrations above 2% the methylene glycol begins to polymerize to form polyoxymethylene glycol, and that these polymers are what react with and crosslink proteins in biological material. My choice of fixative might then be an initial immersion fix in 4% buffered formaldehyde for perhaps 1 hr, followed by a second immersion fix in 8% buffered formaldehyde for 24 hr. I would make sure not to use phosphate buffered saline, but something with a better buffering capacity (such as 100mM phosphate buffer).

Once fixed, I would cut off a small portion of the tissue (carefully so as not to crush the sample), infiltrate with sucrose and prepare cryosections, mount them onto glass coverslips and label them with my antibody and a fluorescent secondary. I could also visualize the bound antibody by reacting with protein A gold and then silver enhancing the bound gold. I have no concern about whether the silver enhancer will work at the EM level because I know I will be able to easily detect the 5nm protein A-gold at the magnifications I use.

Once I had found a part of the tissue containing the mast cells, I would then trim down the block, cool the ultramicrotome and cut thin sections for EM. These I would label with antibody and protein A-gold, confident that the sections contained mast cells and that the immunoreagents would be working.

If I found that the antibody gave unusual results, suggesting either a migration or extraction of antigen, I would take part of the tissue still in aldehyde and embed it in Lowicryl. For this I have the choice of PLT (progressive lowering of temperature) or freeze substitution. As I know that freeze substitution generally gives increased sensitivity, I would probably use that. I would cryoprotect small pieces of sample in sucrose (above 1.6M) and freeze by immersion in liquid nitrogen (only because I do not have a high pressure freezer), and put the frozen blocks in cold methanol (to increase contrast I may add 1% osmium tetroxide or uranyl acetate to the substitution medium). While still cold, I would gradually replace the methanol with unpolymerized Lowicryl until I was in 100% resin and could polymerize it by exposure to UV light, still at low temperature.

The sectioning step would be the same as above. I would first prepare sections for LM to screen the tissue for mast cells and for immunolabeling. Once I had both, I would thin section and look at the sample by EM. If this routine is kept standard then the level of success will increase as the level of frustration drops.

As for your comment that you see background in the first few sections of your EM labeling, I presume that this is outside the cells. It is possible that this background is also there in your LM preparations but because it is only in a thin layer, the LM may not have the sensitivity to register it. If you are getting a high signal to noise ration at the LM level but not by EM, it would be a good idea to figure out why this is. The result may affect the way you interpret your EM pictures.
I notice that many antibodies when tested by LM appear to be very specific with no background, but when used at the EM level, are unusable because of the large amount of background they produce. If the LM is carefully performed and the results examined before they have been exposed to the process I have called "Photoshop purification of antibodies", the background can be picked up there too. Only with very few antibodies have I noticed that no background is detected by LM or by Western blotting, but that it comes up in the EM. Usually this is a result of the high concentrations I use the antibodies at, compared to what is used for other detection emthods. The offending signal, which is still specific, has been diluted out. This background can be eliminated by careful preparation and purification of antibodies.

By all means expose the labeled sections to a fixation step after the labeling protocol is complete. It is such a small part of the process as to be negligible. I would be interested, however, in any quantitative results that actually show this step to retain gold particles when compared with labeling protocols that leave it out.

Regards,

Paul Webster.

Disclaimer: Other than a satisfied user, I have no affiliation with or interest in Lowicryl resins or Adobe Photoshop. All opinions are my own and are freely given. How they are used is not my responsibility.


Paul Webster, Ph.D
House Ear Institute
2100 West Third Street
Los Angeles, CA 90057
phone:213 273 8026
fax: 213 413 6739
e-mail: pwebster-at-hei.org
http://www.hei.org/htm/aemi.htm


Michael Plociniak wrote:
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}
} A response directed to Paul Webster, c/o this invaluable and open forum:
}
} I would like to continue with the topic of immunogold labeling for TEM,
} relating to your suggestion:
}
} "One possible step you could put in is to first try out your whole
} preparation and immunolabeling protocol at the LM level. Use the same
} reagents and same preparation protocols as you plan to use for EM and
} expose to silver enhancment for a longer time so that you can see the
} signal by LM. If you see a signal then you will be confident that you will
} see a signal when you go to examine the EM experiment."
}
} How reliable is LM for predicting TEM immunogold labeling results?
}
} I have tried this approach before, with mixed results. It sounds like a
} great idea, but in attempting to "modify" the EM protocol into a LM pilot
} experiment, it becomes a completely different beast. During primary
} fixation, EM requires glutaraldehyde to preserve ultrastructure. You
} suggested that glut not be used until AFTER immunolabeling, but won't the
} damage to the fine structure already be done? I was under the impression
} that glut "postfixation" is intended primarily to crosslink gold antibodies
} in place after they have bound to primary antibody. }
} Also, silver and gold enhancement reagents come in LM and EM formulae;
} usually, EM versions are less acidic. Does a longer LM enhancement
} accurately predict the action of a brief EM enhancement? Perhaps EM
} formulae can be used to directly monitor enhancement with the light
} microscope. However, because optimal EM enhancement is invisible by LM,
} one will need to empirically determine EM enhancement times for each cell
} system. Doesn't this argue against spending too much time with LM
} experiments, when EM also includes osmium, uranyl acetate, dehydration, and
} resin infiltration?
}
} I still use pilot LM experiments, but more for their complimentary benefit,
} rather than for their predictive value. }
} Routinely, I see a discouraging signal to noise ratio at the LM level.
} However, at the EM level noise is restricted to the first couple of resin
} sections of a monolayer culture, and subsequent sections contain beautiful
} results. I interpret this as nonspecific binding of antibodies to the
} poly-L-lysine and lamin-coated glass substrate. In this example, routine
} LM (DIC optics without optical sectioning) does not accurately predict EM
} performance.
}
} Sincerely,
}
} Michael Plociniak
} Research Technician
} Albert Einstein College of Medicine
} Neuroscience Dept.
} Rose Kennedy Center, 529
} Bronx, NY 10461
} (718) 430-3509
} plocinia-at-aecom.yu.edu
}
} transmission and scanning electron microscopy
}
}
}
}
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Paul Webster, Ph.D
House Ear Institute
2100 West Third Street
Los Angeles, CA 90057
phone:213 273 8026
fax: 213 413 6739
e-mail: pwebster-at-hei.org
http://www.hei.org/htm/aemi.htm




From daemon Tue Sep 26 18:08:58 2000



From: Peter Earl :      petere-at-pathcom.com
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Subject: Unsubscribe

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From daemon Tue Sep 26 18:09:13 2000



From: Chuck Buiocchi :      buiocchi-at-astro.ocis.temple.edu
Date: Tue, 26 Sep 2000 17:56:55 -0500
Subject: Philips 420 TEM Main Transformer wiring hook-up

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I need some help.
If anyone out there has a Philips 420 TEM, I would appreciate some info on
hooking up the main transformer (i.e. from the installation manual or
anything else that I might use), our electrian cann't figure out the wiring
for hooking it up.
Any help would be greatly appreciated.
Thank You,
Chuck





From daemon Tue Sep 26 18:09:13 2000



From: Leah Wajdyk :      leah.wajdyk-at-onsemi.com
Date: Tue, 26 Sep 2000 17:55:59 -0500
Subject: Argon and PIPS contamination

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Hello,
I have just changed the Ar tank that is used on my PIPS. I now have a hugh
amount of contamination from the grid on my TEM samples. The only thing
that has changed is the tank. I made sure the guns are aligned and the flow
adjusted properly.

Has anyone else had this happen after changing the tank and is there some
advice for me?

Leah Wajdyk
On Semiconductor
CSAL TEM







From daemon Tue Sep 26 18:34:41 2000



From: jmkrupp-at-cats.ucsc.edu (Jon Krupp)
Date: Tue, 26 Sep 2000 16:24:47 -0700
Subject: LM-Microslide storage boxes for anyone

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Hi:

I have a bunch of microslide storage boxes, the plastic kind that hold 100
glass slides. They have been used, but are in good condition . I don't need
them any more, let me know if you would like some or away they go.

Jonathan Krupp
Microscopy & Imaging Lab
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu





From daemon Tue Sep 26 20:59:07 2000



From: Becky Holdford :      r-holdford-at-ti.com
Date: Tue, 26 Sep 2000 20:59:40 -0500
Subject: Re: mu-metal

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Mark: I can't comment on a mu-metal shield, but have used field-cancelling
systems on SEMs and FIBs to great effect. I can't remember what we paid for
ours, so I don't know if such a system would cheaper than the shield. But if
you're curious try this URL: http://www.spicerconsulting.com/. This company
is in the UK, but I think they have people in the Americas.

Mark West wrote:

} ------------------------------------------------------------------------
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} Hi,
}
} A question about SEM interference. We have high electromagnetic field
} levels (about 5 times the Jeol recommended limits) and our SEM isn't much
} use above 10 000x mag (a Jeol 5410 low vacuum) - the focus is fuzzy and
} astigmatism hard to correct. I've had water and air pipes removed from the
} room, and we've been everywhere measuring fields but the fields and the
} poor performance don't change. Changing the room is not an option (to the
} people in charge of the institute!). Jeol recommended, ruling out a room
} change, that we fit a mu-metal shield around the column. I have contacted
} the company that makes the mu-metal and the thing would cost about
} US$6000.00 and a hassle to ship to Mexico, so I don't want to go ahead and
} find that we're in the same place with our performance.
}
} Does anyone have experience with fitting a mu-metal shield to their
} microscope (especially a scanning EM) and resolving (or not resolving!)
} interference problems. I'd be very interested in hearing about your
} experiences with this stuff.
}
} Thanks,
}
} Mark
}
} PS - the serial sections are coming along nicely (not perfect,but heading
} that way!), but I've still got a lot of ideas to try yet. Many thanks.
}
} ********************************************
} Mark West,
} Unidad de Microscopia Electronica,
} (Electron Microscopy Unit)
} Instituto de Fisiologia Celular,
} Universidad Nacional Autonoma de Mexico,
} 04510 Mexico D.F.
}
} tel (unidad/lab) *(525) 622 5610*
} (casa/home) (525) 619 3020
} Fax (525) 616 2282
} ********************************************

--
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Becky Holdford (r-holdford-at-ti.com)
972-598-1291 (pager)
KFAB Physical Analysis Lab--SEM/FIB/FA
Texas Instruments, Inc.
Dallas, TX
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~





From daemon Tue Sep 26 22:54:38 2000



From: Hong Yi :      hyi-at-emory.edu
Date: Tue, 26 Sep 2000 23:43:18 -0400 (EDT)
Subject: Re: Immuno TEM

Contents Retrieved from Microscopy Listserver Archives
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In one of his recent postings, Dr. Webster suggested using cell
fractionation for immuno-localization. This pointed to an alternative
path to the usual route I take for immunolabeling. I of course
understand that in some circumstances, this approach might be the only way
to acquire valid data. However, on the other end of the spectrum, there
are also situations, perhaps a majority, in which the localization is only
relevant when the cellular and tissue organization is intact. I work a
lot with brain tissue, in which the membrane integrity is essential for
identifying different neuronal elements, and the labeling technique is
often intended to help figure out the relationship among those elements.
In this case, cell fractionation is certainly not suitable, and even in
intact tissue, the morphology can only be compromised to a certain point.
Inconveniently, this point may well be beyond the threshold that allows
antigen survival or antibody access.

Fortunately, technology has advanced so much thanks to the relentless
efforts of many. With the availability of ultrasmall gold conjugates and
silver enhancement reagents for EM, the detectability of antigens in
fixed, wet tissue with excellent ultrastructure is no longer unreachable.
I routinely use 4% paraformaldehyde plus 0.2% glutaraldehyde to perfuse
brain and then post fix it with the same fixative for 1 hour. The
ultrastructure quality obtained with this fixation is such that there is
no severe dilation of the ER and Golgi, very little swelling of
mitochondia, and most membranes are still intact. Yet, with this
morphology, I have labeled epitopes in the cytoplasm, nuclei, both inside
and outside of Golgi and ER, and on both sides of the cell membranes. Too
bad one is not allowed to post images on this listserver.

There are two stages involved in the final preservation of ultrastructure
during pre-embedding immuno-labeling. The initial fixation determines the
starting point of ultrastructure quality and is limited by the tolerance
level of the antigens. Subsequently, the immunolabeling procedure itself
is responsible for preventing deterioration of the ultrastructure. The
latter is closely related with reagents to be used, and has been the focus
of reagent improvement for the last few years. Using a single Fab
conjugate allows less harsh permeablization of the tissue. A near neutral
pH silver enhancement reagent reduces the harm of pre-osmium enhancement
on morphology.

Will it work at the EM level if it does at the LM level? Pre-embedding
labeling with ultrasmall gold conjugates and silver enhancement has
definitely made it easier to carry LM success to the EM level.

Thank you.

Hong

=============
Hong Yi
Emory Neurology Microscopy Core Laboratory
Emory University School of Medicine
6215 Woodruff Memorial Research Building
1639 Pierce Drive
Atlanta, GA 30322
Tel: (404) 727-8692
Fax: (404) 727-3157
Email: hyi-at-emory.edu




From daemon Wed Sep 27 01:10:13 2000



From: Gunnar Glasser :      glasser-at-mpip-mainz.mpg.de
Date: Wed, 27 Sep 2000 07:58:45 +0200
Subject: .... again: Digital Micrograph???

Contents Retrieved from Microscopy Listserver Archives
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Hi,

.. I am just curious: What's the good thing about DM? "Everbody" seems to
be complaining about this "image format"(?). - What's the advantage of DM
compared to TIFF?


Dipl.Ing.(FH) G.Glasser
Elektronenmikroskopie
Max Planck Institut fuer Polymerforschung
Ackermannweg 10
55128 Mainz, GERMANY
phone: ++49 +6131 379195
fax : ++49 +6131 379100
web: http://www.mpip-mainz.mpg.de/~glasser

Disclaimer: The above statement is mine alone and does not implicitly represent the position of the MPI-P or the MPG!



From daemon Wed Sep 27 01:12:15 2000



From: Darrel Westin :      skacm-at-ragingbull.com
Date: Wed, 27 Sep 2000 00:19:24 -0500
Subject: Your Site #3B53

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From daemon Wed Sep 27 02:52:17 2000



From: Claudia Hayward-Costa :      LS_S562-at-crystal.kingston.ac.uk
Date: Wed, 27 Sep 2000 08:39:47 +0100
Subject: TEM/SEM bacteria protocol

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Dear Kristine

Have you thought about filtering your bacteria on polycarbonate
filters? This method would obviously only work for the SEM.

There was a thread a while ago and I think I copied it from the
archives where I searched for the the keyword "filters". Quite a few
people were discussing how to prepare bacteria for SEM and
nearly all suggested filters.

A typical protocol was: filtering suspension of bacteria through 0.2
um polycarboante filter, remove filter from housing and place into
2.5% glutaraldehyde in buffer, follow with buffer washes, optional
post fixation with OsO4, gradient alcohol series (3 x 100 % EtOH)
and then either 3 washes in HMDS (hexamethyldisilizane) followed
by air drying or critical point drying.

I am working with leukemia cell cultures and pellet the cells for
TEM (my cells are easier to pellet) and filter them on polycarbonate
filters for SEM.

Regards

Claudia
Dr. C. Hayward-Costa
School of Life Sciences
Kingston University
Penrhyn Road, Kingston upon Thames
Surrey KT1 2EE, UK
44(0)208 547 2000 x 2240
Email: c.hayward-at-kingston.ac.uk



From daemon Wed Sep 27 03:54:40 2000



From: Chris Jeffree :      cjeffree-at-srv0.bio.ed.ac.uk
Date: Wed, 27 Sep 2000 09:36:06 +0100
Subject: Testing SEM resolution

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I am looking at Field Emission SEMs with a view to a lasting and
meaningful relationship, and am reminded of the problem of
verifying manufacturer's claims about resolution. I dimly recall
having been here before, but it is such a long time since I had the
luxury of considering SEM purchase that it has become buried in
my deep subconscious, alongside other strongly repressed issues
like full costs recovery, the Oedipus complex, resentment of
academic payscales and Bo Derek.

The more I reflect on resolution the more it looks like a can of
worms. For example, it has recently become apparent that some
FESEMs can "resolve" 1nm gold on a featureless carbon film under
conditions where the manufacturer is only claiming a lesser
resolution. This situation must be analogous to the situation where
15nm gold can be imaged by reflection light microscopy, or stars
are imaged by eye or telescope. In other words the individual point
sources of light (or secondaries) are seen, but are represented by
the imaging system as objects larger than their true size.
Most manufacturers seem to use gold particles on carbon or mag
tape as resolution test objects. I have reservations about this, partly
because of the effect described above, which will result from the
massive contrast between gold and carbon, but also because it is
not a realistic test of performance in the context of low-kV operation
and light-element materials and biological samples.

I would therefore be grateful for your experience and advice on
some of the following issues:

How should resolution be defined?

Is there a rigorous way of measuring the resolution achieved in a
given SEM image?

Is there a rigorous way of establishing the fundamental resolving
power of the SEM imaging system?
(I think these two questions are different)

What would you recommend as a test object for testing the practical
resolution of the sytem in imaging of light-element samples?

Consider the following conditions:

Field width on specimen = 1µm
Magnification = 200k at display width of 200mm (8")
At a digital image size of 1024x768 pixels (typical basic FESEM
display resolution) the pixel size is therefore about 1nm
Presumably at this magnification even if the SEM is resolving 1nm
(let's assume it is) the display system is inadequate to demonstrate
the fact.

Question: How many pixels would be required to image and resolve
the 1nm data in this image? The answer to this question is
presumably influenced by the criteria employed for establishing the
resolution (point to point? peak width at half height? etc) and
presumably dictates either the minimum magnification or the
minimum digital resolution necessary to test resolution at the
approximately 1nm current limit of FEGSEM performance.

I would be grateful for your comments
Chris

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Dr Chris Jeffree
University of Edinburgh
Biological Sciences EM Facility
Daniel Rutherford Building
King's Buildings EDINBURGH EH9 3JH
Tel: +44 (0) 131 650 5345
FAX: +44 (0) 131 650 6563

Inveresk Cottage, 26 Carberry Road,
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Tel: +44 (0) 131 665 6062 / Mobile 07710 585 401
FAX: +44 (0) 131 653 6248
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From daemon Wed Sep 27 07:20:43 2000



From: Shalvoy, Richard B **CHES :      RBShalvoy-at-archchemicals.com
Date: Wed, 27 Sep 2000 07:08:28 -0500
Subject: Dye Sub Printer Speeds - Wrapup

Contents Retrieved from Microscopy Listserver Archives
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My thanks to everyone for their suggestions about how best to troubleshoot
my slow dye sub printer. I can't say that I have gotten a conclusion yet,
but I have learned enough to see that taking the printer off the SEM's
computer is my best bet. It prints fine from every computer I have tried
EXCEPT the SEM one and after some problems I've had with that one, it seems
wise to separate this function from that system. That done, all is fine.

Thanks again to all here who were so generous of their time and ideas.

Richard Shalvoy
Arch Chemicals
Cheshire, CT



From daemon Wed Sep 27 07:30:22 2000



From: Jackie Awane :      skacm-at-ragingbull.com
Date: Wed, 27 Sep 2000 07:15:16 -0500
Subject: Your Status #3C12

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From daemon Wed Sep 27 08:26:35 2000



From: anderron-at-us.ibm.com
Date: Wed, 27 Sep 2000 09:14:03 -0400
Subject: Re: Testing SEM resolution

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A very basic point that is almost always overlooked when discussing SEM
"resolution," is the difference between "resolution" and "resolving power."

An SEM might have a resolving power of 1nm or so based on the observation
of gaps between gold particles on a featureless carbon film, as you point
out. Fine--if your job is to look at gold on C.

For the actual 3-D samples most everyone looks at, EVERY micrograph has a
DIFFERENT resolution, closer to 10nm, because of secondaries generated by
back scattered electrons, edge effects, ... etc. If you want resolutions
closer to the instrument's resolving power you have to look at thin TEM
specimens without sharp edges and angles.

In our semiconductor lab we have several FESEMs advertised to have
resolutions of 1nm that can't resolve 4 and 5 nm layers in a film stack.
There is no question that these instruments are superior to non-FESEMs and
that today's SEMs are feature-filled and wonderfully reliable--just don't
expect 1nm resolution in real images and remember the difference between
resolution and resolving power.

So if every instrument has one "resolving power" and a different
"resolution" for each picture, to answer your question: the only test of
"resolution" for you is to take your bread-and-butter samples to each
manufacturer and compare the resulting images. There isn't any theoretical
formula that will provide a more meaningful answer.

Ron


Ron Anderson, IBM, Hopewell Jct., New York, USA. anderron-at-us.ibm.com

IBM Analytical Services; http://www.chips.ibm.com/services/asg






From daemon Wed Sep 27 08:49:53 2000



From: Augusto.A.Morrone-at-seagate.com
Date: Wed, 27 Sep 2000 08:37:44 -0500
Subject: Argon and PIPS contamination

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Leah:

We haven't changed the Ar tank yet, so I have not observed the problem you
describe. However, we do get Cu contamination periodically in the PIPS due
to buildup of contamination on the bottom surface of the shutter (facing
the sample). This is easily fixed by removing the shutter and cleaning it
with very rough, 320 grit or coarser, grinding paper. The timing may be
coincidental with the change of Ar tank, but just in case check that the
presure is set correctly in the tank regulator (8 psi) and the gas control
valves; a higher pressure may have increased the Ar in the beam and the
sputtering from the shutter to the sample. The most common source of
contamination from the grid is the grid itself: grid thickness varies, and
when it is larger than nominal the grid "shadows" the sample at low milling
angles sputtering grid material onto the sample. I hope this helps.

Augusto Morrone
Seagate Technology
Augusto_A_Morrone-at-notes.seagate.com





"Leah Wajdyk" {leah.wajdyk-at-onsemi.com} on 09/26/2000 05:55:59 PM

To: Microscopy-at-sparc5.microscopy.com
cc:



Hello,
I have just changed the Ar tank that is used on my PIPS. I now have a hugh
amount of contamination from the grid on my TEM samples. The only thing
that has changed is the tank. I made sure the guns are aligned and the
flow
adjusted properly.

Has anyone else had this happen after changing the tank and is there some
advice for me?

Leah Wajdyk
On Semiconductor
CSAL TEM












From daemon Wed Sep 27 09:15:00 2000



From: Andrew Ochalski :      aochalsk-at-science.uottawa.ca
Date: Wed, 27 Sep 2000 10:04:02 -0500
Subject: Sticking vesicles to glass coverslips

Contents Retrieved from Microscopy Listserver Archives
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Dear Listmembers,

There is a group here that would like to use digital microscopy to characterize sizes of
vesicles obtained by fractionation of myocytes. The problem is to get them to stick to
glass coverslips in a non-selective way. Because we had it on hand, we tried poly-L-lysine and
letting the vesicles settle overnight at 4C. The proportion of material that remained
on the coverslips was very small. I have a few questions:
1) Is it possible that different populations (with different membrane proteins etc.) would adhere
differentially to the positively-charged surface?
2) Does anyone have experience with different substrates (e.g. Cel-Tak, silane, albumin) that would
be more appropriate.
3) Should we abandon this approach and use the more reliable (?) negative stain?EM route.(I worry
about shrinkage artifacts due to air-drying and heavy-metal stains)
Any advice will be gratefully considered and references appreciated. Thanks in advance.
Regards,

Andrew Ochalski,
Microscopy Technician,
Dept. of Biology,
University of Ottawa
Room 108, Gendron Bldg.
130 Louis Pasteur,
Ottawa, Ontario
CANADA
K1N 6N5
613-562-5800 x6343
FAX: 613-562 5486



From daemon Wed Sep 27 09:16:43 2000



From: flaitz-at-us.ibm.com
Date: Wed, 27 Sep 2000 10:06:41 -0400
Subject: Re: Argon and PIPS contamination

Contents Retrieved from Microscopy Listserver Archives
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An additional consideration for any low angle ion milling is to change to
Mo grids. They have a much lower sputter rate. They are also thinner,
allowing a much lower milling angle on the grid side of the specimen.
Using 1 mm diameter grids, and with the feature of interest cnetered in the
hole, we routinely mill as low as 3 degrees into the grid hole. They are
particularly suitable for specimens which require extended low angle
milling, on the order of 2-3 hours.

Phil

Philip L. Flaitz
IBM Analytical Services, Hopewell Junction, NY
http://www.chips.ibm.com/services/asg
Ph.......(914) 892-3094, FAX -2003
flaitz-at-us.ibm.com




From daemon Wed Sep 27 09:37:18 2000



From: Tyrone L. Daulton :      tdaulton-at-nrlssc.navy.mil
Date: Wed, 27 Sep 2000 09:24:27 -0500
Subject: DM File format

Contents Retrieved from Microscopy Listserver Archives
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The Digital Micrograph (DM) format is not a image file format for say in the way that TIFF, PICT, JPEG, GIF, etc. are
file formats (some of which use lossy compression). Digital Micrograph, in addition to saving the image (with no
reduction in data), saves a lot of experimental header information, for instance calibrations, images size, keywords,
magnification, electron energy, TEM operator name, image annotations etc. Many of these can be entered in the Object
Info dialog. Also, DM can save multiple images in a single DM file.

Tyrone Daulton


Gunnar Glasser wrote:

} Hi,
}
} .. I am just curious: What's the good thing about DM? "Everbody" seems to
} be complaining about this "image format"(?). - What's the advantage of DM
} compared to TIFF?
}
} Dipl.Ing.(FH) G.Glasser
} Elektronenmikroskopie
} Max Planck Institut fuer Polymerforschung
} Ackermannweg 10
} 55128 Mainz, GERMANY
} phone: ++49 +6131 379195
} fax : ++49 +6131 379100
} web: http://www.mpip-mainz.mpg.de/~glasser
}
} Disclaimer: The above statement is mine alone and does not implicitly represent the position of the MPI-P or the MPG!

--
_____________________________________________________
Tyrone L. Daulton
Director: Marine Geosciences - Electron Microscopy Center
Physicist and Senior Electron Microscopist

Naval Research Laboratory
Marine Geosciences Division (Code 7400)
Stennis Space Center, MS 39529-5004

Voice (228)-688-4877
Fax (228)-688-4093
email tdaulton-at-nrlssc.navy.mil
tld-at-howdy.wustl.edu





From daemon Wed Sep 27 09:38:38 2000



From: John Henry J. Scott :      johnhenry.scott-at-nist.gov
Date: Wed, 27 Sep 2000 10:27:28 -0400
Subject: TEM,uProbe: NIST/MAMAS Meeting Oct 19, 2000

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Meeting Announcement

NIST/MAMAS Meeting

The Mid-Atlantic Microbeam Analysis Society and the NIST Surface and
Microanalysis Science Division will be meeting at the National Institute
of Standards and Technology Gaithersburg, MD on

Thursday, October 19, 2000
10:15 am to 3:00 pm
NIST Administration Building
Lecture Room D

10:30 am
Energy-Filtered TEM
Jim Bentley
Oak Ridge National Laboratory
2000-2001 MAS Tour Speaker

1:00 pm
Electron Microprobe Dating of Minerals
John Hanchar
Department of Earth and Environmental Sciences
The George Washington University

For more information, contact
John Henry Scott 301-975-4981, johnhenry.scott-at-nist.gov, or
Ryna Marinenko 301-975-3901, ryna.marinenko-at-nist.gov
Don't forget to bring your MAMAS dues ($5.00)

Hope to see you there,
-- John Henry

John Henry J. Scott Bldg 222/Rm A113
NIST Microanalysis Research Group 100 Bureau Drive Stop 8371
(301) 975-4981; (301) 417-1321 FAX Gaithersburg, MD 20899-8371



From daemon Wed Sep 27 09:46:18 2000



From: Norm Olson :      nho-at-bilbo.bio.purdue.edu
Date: Wed, 27 Sep 00 09:35:01 -0500
Subject: Fwd: .... again: Digital Micrograph???

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------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


{Subject: .... again: Digital Micrograph???
{From: Gunnar Glasser, glasser-at-mpip-mainz.mpg.de
{.. I am just curious: What's the good thing about DM? "Everbody" seems
to
{be complaining about this "image format"(?). - What's the advantage of
DM
{compared to TIFF?

It is a proprietary format, no other common program can use it. In an
earlier post I say that I save everything in Digital Micrograph format
and that is my archival image. If you have properly calibrated the mag
on your camera and you spend time putting in keywords, etc, these become
a permanent part of the image and you can always go back and get that
image. I always try to be as verbose as I can with information. For
example, my image name may be:
000927rhinovirus.1 which has today's date and a short sample name. This
also allows me to sort files by a name that can be chronologically based.
In the tags and keywords I have the pixel size (based on mag), sample
name, collaborator's name, sample prep conditions, etc., etc. You can
also measure items automatically without having to calculate in your mind
that x pixels in the image equals y nm in the sample. You cannot do that
with a TIFF or whatever. After I have saved my image in DM format, then
I will resave it again, if I want to, in any other format but I always
have the untouched original to fall back to.

Norm Olson

*******************************************
Norm Olson
Senior Research Electron Microscopist
Department of Biological Sciences
Lilly Hall of Life Sciences
Purdue University
West Lafayette, IN 47907

Phone: 765-494-5643
FAX: 765-496-1189
email: nho-at-bragg.bio.purdue.edu
http://bilbo.bio.purdue.edu/~nho/index.htm
http://bilbo.bio.purdue.edu/~baker/

*******************************************




From daemon Wed Sep 27 09:57:13 2000



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Wed, 27 Sep 2000 15:46:27 +0100 (GMT Daylight Time)
Subject: Re: TEM/SEM bacteria protocol

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Claudia, do you notice any difference in your cell cultures
between HMDS and CPD?

Dave


On Wed, 27 Sep 2000 08:39:47 +0100 Claudia Hayward-Costa
{LS_S562-at-crystal.kingston.ac.uk} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear Kristine
}
} Have you thought about filtering your bacteria on polycarbonate
} filters? This method would obviously only work for the SEM.
}
} There was a thread a while ago and I think I copied it from the
} archives where I searched for the the keyword "filters". Quite a few
} people were discussing how to prepare bacteria for SEM and
} nearly all suggested filters.
}
} A typical protocol was: filtering suspension of bacteria through 0.2
} um polycarboante filter, remove filter from housing and place into
} 2.5% glutaraldehyde in buffer, follow with buffer washes, optional
} post fixation with OsO4, gradient alcohol series (3 x 100 % EtOH)
} and then either 3 washes in HMDS (hexamethyldisilizane) followed
} by air drying or critical point drying.
}
} I am working with leukemia cell cultures and pellet the cells for
} TEM (my cells are easier to pellet) and filter them on polycarbonate
} filters for SEM.
}
} Regards
}
} Claudia
} Dr. C. Hayward-Costa
} School of Life Sciences
} Kingston University
} Penrhyn Road, Kingston upon Thames
} Surrey KT1 2EE, UK
} 44(0)208 547 2000 x 2240
} Email: c.hayward-at-kingston.ac.uk
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"




From daemon Wed Sep 27 10:16:20 2000



From: craig_wall-at-goodyear.com
Date: Wed, 27 Sep 2000 10:53:11 -0400
Subject: Re: DM File format

Contents Retrieved from Microscopy Listserver Archives
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The encoding of the image information in DM is the TIFF format, with
the extra header info listed below. It is possible on files containing
single images to strip the header info and open them with Photoshop.

Craig Wall

*%*#*%*#*%*#*%*#*%*#*%*#*%*#
Disclaimer: The above statements are mine alone and do not implicitly
represent the position of the Goodyear Tire & Rubber Co.
Craig G. Wall, Ph.D.
The Goodyear Tire & Rubber Company
142 Goodyear Boulevard
Akron, Ohio 44305
(330)796-2335
(330)796-3304 FAX









The Digital Micrograph (DM) format is not a image file format for say in
the way that TIFF, PICT, JPEG, GIF, etc. are
file formats (some of which use lossy compression). Digital Micrograph, in
addition to saving the image (with no
reduction in data), saves a lot of experimental header information, for
instance calibrations, images size, keywords,
magnification, electron energy, TEM operator name, image annotations etc.
Many of these can be entered in the Object
Info dialog. Also, DM can save multiple images in a single DM file.

Tyrone Daulton


Gunnar Glasser wrote:

} Hi,
}
} .. I am just curious: What's the good thing about DM? "Everbody" seems to
} be complaining about this "image format"(?). - What's the advantage of DM
} compared to TIFF?
}
} Dipl.Ing.(FH) G.Glasser
} Elektronenmikroskopie
} Max Planck Institut fuer Polymerforschung
} Ackermannweg 10
} 55128 Mainz, GERMANY
} phone: ++49 +6131 379195
} fax : ++49 +6131 379100
} web: http://www.mpip-mainz.mpg.de/~glasser
}
} Disclaimer: The above statement is mine alone and does not implicitly
represent the position of the MPI-P or the MPG!

--
_____________________________________________________
Tyrone L. Daulton
Director: Marine Geosciences - Electron Microscopy Center
Physicist and Senior Electron Microscopist

Naval Research Laboratory
Marine Geosciences Division (Code 7400)
Stennis Space Center, MS 39529-5004

Voice (228)-688-4877
Fax (228)-688-4093
email tdaulton-at-nrlssc.navy.mil
tld-at-howdy.wustl.edu










From daemon Wed Sep 27 10:18:27 2000



From: Hao Li :      haoli-at-eng.umd.edu
Date: Wed, 27 Sep 2000 11:09:13 -0400
Subject: Cross section cleavage of MgO

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Hi, anybody has experiences on small angle(or 90 degree) cleavage method of
making cross section TEM samples with MgO substrates? Any information would
be appreciated.

Thanks a lot

Hao Li




From daemon Wed Sep 27 12:23:37 2000



From: Frank Thomas :      thomasf-at-AGC.BIO.NS.CA
Date: Wed, 27 Sep 2000 14:07:16 -0300
Subject: Re: .... again: Digital Micrograph???

Contents Retrieved from Microscopy Listserver Archives
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----------
} From: Norm Olson {nho-at-bilbo.bio.purdue.edu}
} To: MSA {Microscopy-at-sparc5.microscopy.com}
} Subject: Fwd: .... again: Digital Micrograph???
} Date: September 27, 2000 11:35 AM
}

}
}
} {Subject: .... again: Digital Micrograph???
} {From: Gunnar Glasser, glasser-at-mpip-mainz.mpg.de
} {.. I am just curious: What's the good thing about DM? "Everbody" seems
} to
} {be complaining about this "image format"(?). - What's the advantage of
} DM
} {compared to TIFF?
}
} It is a proprietary format, no other common program can use it. In an
} earlier post I say that I save everything in Digital Micrograph format
} and that is my archival image. If you have properly calibrated the mag
} on your camera and you spend time putting in keywords, etc, these become
} a permanent part of the image and you can always go back and get that
} image. I always try to be as verbose as I can with information. For
} example, my image name may be:
} 000927rhinovirus.1 which has today's date and a short sample name.
................After I have saved my image in DM format, then
} I will resave it again, if I want to, in any other format but I always
} have the untouched original to fall back to.
}
} Norm Olson
}

For what it's worth, our NORAN Voyager system has its own proprietary
image format called a .grey (theoretically readable only by Voyager
systems), which also incorporates important information such as pixel size,
magnification, date, and whatever other notes you wish to include. I'm told
by one of my colleagues that with a little creative backstripping of
headers, and a foreknowledge of the size of the file, you can open these
things in Photoshop, or CorelDraw or whatever. So far I haven't really had
to do anything like that - our images all get stored both as .greys, and as
.tiffs. With the Voyager software, you can always make a new .tiff from a
.grey, but you can't make a .grey from a .tiff. These proprietary image
formats can be a bit of a pain sometimes, but at least they do allow the
inclusion of all that extraneous information.
In our lab, when a job is finished, the client gets a CD containing all
their images in both formats and I make a duplicate for myself, as an
archive. Theoretically, the client can't look at the .greys. The client's
CD then becomes an off-site archive copy for me, should mine turn out to be
a dud (hasn't happened yet, but I remain pessimistic). If the client is
from "outside", there may be ownership/confidentiality issues involved, so
I always check to see if they mind me keeping an archive for them. So far,
they've all been happy to have me keep a copy, just in case. But then, I'm
not doing any work for military or spy agencies, or even big corporations,
or anybody like that.

F.C. Thomas
Geological Survey of Canada (Atlantic)
Bedford Institute of Oceanography
Dartmouth, Nova Scotia
Canada
B2Y 4A2



From daemon Wed Sep 27 12:23:37 2000



From: Kristian Ukkonen :      kukkonen-at-cc.hut.fi
Date: Wed, 27 Sep 2000 19:51:53 +0300
Subject: Re: TEM CCD camera

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"William F. Tivol" wrote:
} My experience with Peltiers does not involve a CCD camera, but it may be
} instructive nonetheless. When we wanted to provide a constant temperature for
} the chemicals in the darkroom--which required sometimes heating the water and at
} other times cooling it--we decided that Peltiers were the best solution. We
} bought a commercial thermoregulating unit which sent current of the appropriate
} polarity through the Peltiers to adjust the temperature to the desired setpoint.
} The Peltiers failed at an expensive rate, and we found that the thermoregulator
} was such that it provided a specific current, which it turned on and off in a
} duty cycle (the greater the temperature difference, the greater fraction of time
} the current was on). The sudden changes in current were the cause of the
} Peltiers' demise.

Sorry, but that seems not to be the case.

http://www.tellurex.com/resource/txfaq.htm

"Can I use pulse-width modulation to control my Peltier device if I
keep the voltage at VMax or below? Yes, and this is one of the most
electrically-efficient ways to control voltage to your device—although
you must observe some precautions."

And they suggest 2kHz PWM frequency.

I think your temperature regulator supplied too much voltage
or current to the peltier, causing overheating and destruction.
Just a guess.

} We were also warned that moisture in the Peltiers could lead to an
} electrochemical process which caused corrosion and subsequent failure.

This is valid. Water inside peltier will cause both mechanical
trouble (freezing inside peltier), and corrosion problems etc..

To the original poster:
I _really_ think you should think of some way to make certain
that there either is water flow _always_ to the peltier, or
that power to peltier (and camera?) is switched off at power/flow
failure. The latter (switch off) should be easier. The peltier
_WILL_ die from overheating if there is not sufficient cooling.
It might kill your camera by overheating it as well.

If you use just water (not glycol or something) as coolant in
recirculators, perhaps you could add redundancy by adding a
couple of magnetic valves to the peltier cooling lines so
that while there is mains current, the valves lets recirculator
water to flow through peltier, and otherwise let water from water
mains to flow through peltier and to sink. Something like this:


water mains } ----------- valve1 -----o
|
o--------} peltier in
|
recirculator out } ------ valve2 -----o

recirculator in {------- valve3 -----o
|
o-------- { peltier out
|
sink {------------------ valve4 -----o


Where:
valve1: normally open (NO = conducts when coil NOT powered)
valve2: normally closed (NC = conducts when coil IS powered)
valve3: normally closed
valve4: normally open

Just connect all valve coils in parallel and to circuit below.

mains phase ----o--NObutton----o---relaycoil------o----main neutral
| | |
o--NOcontact1--o |
| |
o-(to valve coils) |
|
(from valve coils)---NO contact2---o

The idea is to switch on the system (ie. recirculator flow) with the
button. With relay switched on, contacts 1 and 2 are closed and power
goes to valves. Next, when mains power goes off, relay is nolonger
switched on, and no power goes to valve coils (thus mains water
cooling).
User has to press button again to put relay on - return of mains power
won't do this.

Total cost for valves etc. about 100usd or less.

Of course, if you are using glycol or some other chemical, it might
well be that regulations forbid you from mixing piping of water mains
with your glycol piping, because of danger of contaminating water mains.

STILL, I really think it would be electrically trivial to make
certain that the camera does NOT switch on when there is no coolant
flow (or simpler yet, no mains - just use circuit above with
camera connected instead of valve coils). A simple pressure or flow
switch at coolant line, and power to camera via the pressure/flow
switch will be easy.. This should be pretty fool-proof protection for
the camera in _any_ case of coolant flow failure. I think sooner or
later you _will_ have coolant failure without mains failure
(recirculator
pump failure or something), and this should be anticipated in your
design. Of course, you could as well add flow/pressure switch NOcontact
in series with relaycoil in circuit above.

Just some ideas,

Kristian Ukkonen.



From daemon Wed Sep 27 13:52:43 2000



From: Sara Miller :      saram-at-duke.edu
Date: Wed, 27 Sep 2000 14:31:56 -0400 (EDT)
Subject: Re: Sticking vesicles to glass coverslips

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On Wed, 27 Sep 2000, Andrew Ochalski wrote:

} Date: Wed, 27 Sep 2000 10:04:02 -0500
} From: Andrew Ochalski {aochalsk-at-science.uottawa.ca}
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Sticking vesicles to glass coverslips
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} Dear Listmembers,
}
} There is a group here that would like to use digital microscopy to characterize sizes of
} vesicles obtained by fractionation of myocytes. The problem is to get them to stick to
} glass coverslips in a non-selective way. Because we had it on hand, we tried poly-L-lysine and
} letting the vesicles settle overnight at 4C. The proportion of material that remained
} on the coverslips was very small. I have a few questions:
} 1) Is it possible that different populations (with different membrane proteins etc.) would adhere
} differentially to the positively-charged surface?
} 2) Does anyone have experience with different substrates (e.g. Cel-Tak, silane, albumin) that would
} be more appropriate.
} 3) Should we abandon this approach and use the more reliable (?) negative stain?EM route.(I worry
} about shrinkage artifacts due to air-drying and heavy-metal stains)
} Any advice will be gratefully considered and references appreciated. Thanks in advance.
} Regards,
}
} Andrew Ochalski,
} Microscopy Technician,
} Dept. of Biology,
} University of Ottawa
} Room 108, Gendron Bldg.
} 130 Louis Pasteur,
} Ottawa, Ontario
} CANADA
} K1N 6N5
} 613-562-5800 x6343
} FAX: 613-562 5486
}
Not sure if this will help, but it's worth a try. Many years ago, we did
SEM of red blood cells and needed to have them stuck to a substrate. We
treated glass coverslips with collagen, added the RBCs, and glued them
down with glutaraldehyde. They stuck very well.

Coverslips
Chip one edge of a cover slip (we used 9 x 9 mm) in a specific corner so
that you will be able to know which side is up if it gets flipped over.
_______
| |
| |
| c
|______|

You can do this by holding the cover slip between thumb and forefinger of
one hand and gently dragging the tips of an old pair of EM forceps across
the edge at a downward angle. We had about 70% sucess without breaking.

Acetone clean cover slips and let air dry. Put them into a moist chamber
(Petri dish with wet filter paper) and add a large drop of 1% aqueous
collagen. Note the orientation of your chip mark on the cover slip. Don't
let the drop flow over the sides, but just sit there and rounded up.

Incubate 30 min.

Remove drop of collagen with Pasteur pipette for future use.

Wash coverslips well with dH2O. Can use right away or store dried for
future use.

Add membrane prep, cells, etc., without letting drop run over the sides.
(Surface tension will allow you to cover the glass with a rounded-up
drop.) Do not add too much sample; leave enough room to add more fluid
later. Let sit 30 min.

Then add to the suspension a drop of concentrated glutaraldehyde. We
added 50 ul of 25% glut in PBS to the cell suspension without letting the
drop of suspension flow over the coverslip edge. Do this very slowly so
as not to disturb the stuck material. (The glut is a di-aldehyde that
links things together; in your case, hopefully, the material to the cover
slip. Immunologists use this method to link antigens or antibodies to
solid substrates.)

Let sit another 30 min, then GENTLY wash off the suspension. (We then
critical point dried for SEM, but you can do your routine prep from here.)


Collagen
This stuff comes as a flaky powder. Weigh out 0.1 gram and add to 10 ml
dH-at-O. Stir overnight in the cold (cold to minimize bacterial growth).
Filter through Whatman or any kind of filter paper to remove undissolved
chunks. Keep refrigerated. Can autoclave if long term storage desired.


Good luck.


Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265




From daemon Wed Sep 27 14:35:44 2000



From: Vickie Frohlich :      frohlich-at-uthscsa.edu
Date: Wed, 27 Sep 2000 14:31:26 -0500
Subject: Faculty Positions Available

Contents Retrieved from Microscopy Listserver Archives
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Department of Cellular & Structural Biology
The University of Texas Health Science Center at San Antonio


The Department of Cellular and Structural Biology is recruiting faculty for
tenure track positions at the Assistant, Associate and Full Professor
level. The Department is particularly interested in recruiting qualified
applicants with interests in cell and molecular biology, aging, cancer,
immunology or neurobiology, and who utilize optical imaging approaches as a
main research tool. The ability/willingness to teach in one of the
Department's professional and/or graduate courses is also required.

Interested individuals should submit their curriculum vitae, a statement of
research and teaching interests and experience, and three letters of
recommendation to:

Dr. Ellen Kraig
Chair, Cellular & Structural Biology Faculty Search Committee
The University of Texas Health Science Center
7703 Floyd Curl Drive
Mail Code 7762
San Antonio, Texas 78229-3900

Applications received prior to December 1, 2000 will be considered.

Further information about the Department and its optical imaging facility
can be found at: http://www.uthscsa.edu/csb/csbhome.html

The University of Texas is an Equal Employment Opportunity/Affirmative
Action Employer.






From daemon Wed Sep 27 16:02:34 2000



From: =?iso-8859-1?q?iman=20moradi?= :      sinapooyesh-at-yahoo.com
Date: Wed, 27 Sep 2000 13:49:54 -0700 (PDT)
Subject: cooperation&development

Contents Retrieved from Microscopy Listserver Archives
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Dear sirs
Respectfuly,i beg to let you now that i am a 26 year
old Iranian researcher.Since 1993 to present i have
been ivolved in research activities.I am the director
of an Iranian research institute .One of the main
activities of whichis to operate departments of
electron microscopes.Also i am responsible electron
microscope department of the Isfahan university of
medical scienceis(one of the most reputed universities
of Iran).Meanwhile i am in close relations with other
electron microscope departments all through the
country.
Unfortunately hoewer, in Iran ,in spite of the
existence of a great number of electron
microscopes,the science of electron microscopy is not
very developed and most of the electron microscope
departments are
eather inactive or have little activities.Therefore i
intend to stablish the first center for development of
electron microscopy technology in Iran by the
assistance of the private sector.In this connection i
will very
much appreciate receiving assistance from your
center.I intend to pass a complementary course in the
field of electron microscopy at your center and then
developing the above mentioned center in one of the
cities or international free zones of Iran under
support and assistance of your center .The programs of
this center will include accepting electron
microscopy students from Iran or your country,
instruction of applied electron microscopy,instruction
of electron microscopy courses at different Iranian
universities,helding electron microscopy
workshops,operating electron microscopy
departments,supplying
electron microscopy equipments and consumption
electron microscopy materials,performing different
electron microscopy techniques,praviding expanded
electron microscopy services to scientific researche,
industrial and medical diagnosis departments.
With regard to the acceptance that receives the
activities of our institute in the field of electron
microscopy
it seems that in the future the activities of the
center for development of electron microscopy
technology will
be welcomed in a similar way by both public and
private sectors.
Therfore i would like to ask you to advise me in the
above mentional fields.port of my executive and
research cactivities are as follows:
1.Director of Isfahan's Sinapooyesh institute of
research .
2.Responsibility of electron microscope department of
Isfahan university of medical scienceis.
3.Responsibility of processing system of electron
microscope department at Shahid Beheshti university
of medical scienceisof Tehran.
4.Membership of the Iranian national standard
commitee.
5.Iam the first provider of nanotechnology in Iran.
6.Receiving the appreciation medallion of the twelve
th Kharazmi research festival in Iran.
7.Recieving the first prize of the national young
researchers festival in Iran.
8.Reseiving the special prize at the national congress
of the security of communications of Iran.
9.Instruction of electron microscopy courses at MSc
and phD levels in reputed universitesof Iran.
10.Participaton in several national and international
research congresses and presenting several scientific
articles and activities.
Meanwhile,from 1993 to 1995 i have studied in the
field of medical diagnosis and then from 1995 to 1998
i have learnt electron microscopy techniques at
Isfahan university of medical scienceis .since 1998 to
present
i am pursuing my studies in the field of electronic
engineering.
Thank you very much in advance for your sincer
assistance and advice in this regard.
sincerely yours
Iman moradi


__________________________________________________
Do You Yahoo!?
Send instant messages & get email alerts with Yahoo! Messenger.
http://im.yahoo.com/



From daemon Wed Sep 27 16:12:08 2000



From: Gordon Vrololjak :      gvrdolja-at-nature.Berkeley.EDU
Date: Wed, 27 Sep 2000 14:00:36 -0700 (PDT)
Subject: Re: .... again: Digital Micrograph???

Contents Retrieved from Microscopy Listserver Archives
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There is No advantage to digital micrograph over other formats. It is a
format supported by Gatan by force feeding it to users of their
software. You can jump through some hoops and get it to export images in
other formats though.

\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
Gordon Ante Vrdoljak Electron Microscope Lab
ICQ 23243541 http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
gvrdolja-at-nature.berkeley.edu UC Berkeley
phone (510) 642-2085 Berkeley CA 94720-3330
fax (510) 643-6207 cell (510) 290-6793

On Wed, 27 Sep 2000, Gunnar Glasser wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi,
}
} .. I am just curious: What's the good thing about DM? "Everbody" seems to
} be complaining about this "image format"(?). - What's the advantage of DM
} compared to TIFF?
}
}
} Dipl.Ing.(FH) G.Glasser
} Elektronenmikroskopie
} Max Planck Institut fuer Polymerforschung
} Ackermannweg 10
} 55128 Mainz, GERMANY
} phone: ++49 +6131 379195
} fax : ++49 +6131 379100
} web: http://www.mpip-mainz.mpg.de/~glasser
}
} Disclaimer: The above statement is mine alone and does not implicitly represent the position of the MPI-P or the MPG!
}
}




From daemon Wed Sep 27 17:50:18 2000



From: Ken Tiekotter :      tiekotte-at-up.edu
Date: Wed, 27 Sep 2000 17:35:56 -0500
Subject: GATAN Digiscan

Contents Retrieved from Microscopy Listserver Archives
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Dear ALL,

Do any of you have experience with the GATAN DigiScan hardware and Digital
Micrograph for converting a non-digital sem to a digital sem? We have a
JEOL T200 we are considering for conversion. Your responses are greatly
appreciated.

Sincerely,
Ken Tiekotter, Adjunct Professor
The University of Portland
Department of Biology
5000 N. Willamette Blvd.
Portland, OR 97203

email: tiekotte-at-up.edu





From daemon Wed Sep 27 18:16:01 2000



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Wed, 27 Sep 2000 17:00:00 -0600
Subject: DM File format

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


A bit of additional information:

Tyrone is correct in saying that some of the file formats in circulation
(for example JPEG) use lossy compression and one should be careful with
them. TIFF, however, is not necessarily one of them. TIFF usually allows to
select compression options, which can include lossless compression (LZW).
Also, TIFF is a flexible format where additional information can be stored
in so-called "tags" (hence the name: Tagged Image File Format). There are
public tags (like image size, etc), but each software can implement private
tags, which are just ignored by other software. That way it is possible to
incorporate additional information into the file itself without making it
proprietary. That's the strategy we have pursued. TIFF, apparently like DM,
can also store multiple pages in one file. Other formats, like BMP, only
encode the actual image data and one needs additional mechanisms like a
database to keep track of other elements like keywords.

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Tyrone L. Daulton [mailto:tdaulton-at-nrlssc.navy.mil]
Sent: Wednesday, September 27, 2000 8:24 AM
To: Microscopy-at-sparc5.microscopy.com


The Digital Micrograph (DM) format is not a image file format for say in
the way that TIFF, PICT, JPEG, GIF, etc. are
file formats (some of which use lossy compression). Digital Micrograph, in
addition to saving the image (with no
reduction in data), saves a lot of experimental header information, for
instance calibrations, images size, keywords,
magnification, electron energy, TEM operator name, image annotations etc.
Many of these can be entered in the Object
Info dialog. Also, DM can save multiple images in a single DM file.

Tyrone Daulton


Gunnar Glasser wrote:

} Hi,
}
} .. I am just curious: What's the good thing about DM? "Everbody" seems to
} be complaining about this "image format"(?). - What's the advantage of DM
} compared to TIFF?
}
} Dipl.Ing.(FH) G.Glasser
} Elektronenmikroskopie
} Max Planck Institut fuer Polymerforschung
} Ackermannweg 10
} 55128 Mainz, GERMANY
} phone: ++49 +6131 379195
} fax : ++49 +6131 379100
} web: http://www.mpip-mainz.mpg.de/~glasser
}
} Disclaimer: The above statement is mine alone and does not implicitly
represent the position of the MPI-P or the MPG!

--
_____________________________________________________
Tyrone L. Daulton
Director: Marine Geosciences - Electron Microscopy Center
Physicist and Senior Electron Microscopist

Naval Research Laboratory
Marine Geosciences Division (Code 7400)
Stennis Space Center, MS 39529-5004

Voice (228)-688-4877
Fax (228)-688-4093
email tdaulton-at-nrlssc.navy.mil
tld-at-howdy.wustl.edu





From daemon Thu Sep 28 10:19:49 2000



From: NPGSlithography-at-aol.com
Date: Thu, 28 Sep 2000 11:05:55 EDT
Subject: Re: mu-metal

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The prices I have heard for field canceling systems has ranged from ~US$15k
to $30k. A list of suppliers is available at
"www.jcnabity.com/links.htm#Magnetic Field Cancellation".

Joe
_________________________________________
Joe Nabity, Ph.D.
JC Nabity Lithography Systems
E-Beam Lithography using Commercial SEMs & STEMs
PO Box 5354, Bozeman, MT 59717 USA
Voice: (406) 587-0848
FAX: (406) 586-9514
E-mail: info-at-jcnabity.com
Web: www.jcnabity.com

In a message dated 9/27/2000 3:30:27 AM Mountain Daylight Time,
r-holdford-at-ti.com writes:

} Mark: I can't comment on a mu-metal shield, but have used field-cancelling
} systems on SEMs and FIBs to great effect. I can't remember what we paid
for
} ours, so I don't know if such a system would cheaper than the shield. But
} if
} you're curious try this URL: http://www.spicerconsulting.com/. This
} company
} is in the UK, but I think they have people in the Americas.
}





From daemon Thu Sep 28 10:19:50 2000



From: Anthony Garratt-Reed :      tonygr-at-mit.edu
Date: Thu, 28 Sep 2000 11:03:16 -0400
Subject: Microtome available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Netters:

I have surplus a LKB Ultratome V microtome in full working order. This
would be available free to an academic institution (you pay shipping) or
for a reasonable cost (offers?) to a commercial lab. It is an older
instrument but still produces excellent thin sections at room temperature.
We have replaced it with a new instrument with cryogenic capability.

Obviously, the closer you are to Cambridge, Massachusetts, the easier
shipping would be!

Contact me directly if you have interest or any questions.

Tony Garratt-Reed.



** Anthony J. Garratt-Reed
** MIT Room # 13-1027
** 77 Massachusetts Avenue
** Cambridge, MA 02139-4307
** USA
**
** Phone: (+) 1-617-253-4622
** Fax: (+) 1-617-258-6478
**





From daemon Thu Sep 28 10:26:56 2000



From: ken blight :      blight-at-icrf.icnet.uk
Date: Thu, 28 Sep 2000 16:15:38 +0100
Subject: Cell synchronisation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am interested in synchronising Hela cells and collecting samples in each
stage of Mitosis. Does anybody have a protocol and the times at which each
stage is reached after release from G1?

Ken Blight
Senior Scientific Officer
Electron Microscopy Unit
Imperial Cancer Research Fund
London
England





From daemon Thu Sep 28 11:41:30 2000



From: Augusto.A.Morrone-at-seagate.com
Date: Thu, 28 Sep 2000 11:26:39 -0500
Subject: Argon and PIPS contamination, Correction

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Leah and Listers:

In an early posting I wrongly suggested that the Ar pressure in the PIPS
should be set at 8psi:

"...The timing may be coincidental with the change of Ar tank, but just in
case check that the presure is set correctly in the tank regulator (8 psi)
and the gas control valves; a higher pressure may have increased the Ar
.."

Neal Zimmermann, who works in my lab, corrected me: the pressure should
be set to 25psi as stated in the Gatan PIPS manual. Fortunately we had it
set right, just my memory lapsed.

Augusto

Augusto Morrone
Seagate Technology
Augusto_A_Morrone-at-notes.seagate.com




From daemon Thu Sep 28 15:33:40 2000



From: Paul Webster :      pwebster-at-hei.org
Date: 28 Sep 00 13:13:35 -0700
Subject: RE: Immuno LM/EM

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From: Paul Webster :      pwebster-at-hei.org
Date: 28 Sep 00 13:13:35 -0700
Subject: RE: Immuno LM/EM

Contents Retrieved from Microscopy Listserver Archives
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------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Reply to: RE: Immuno LM/EM
Hi Jan,

Thanks for joining in. I was beginning to feel a bit lonely out here. It is interesting, however, to read the many off-line relplies I have been getting about this posting. Obviously many people are struggling with there attempts to understand the many different choices available for immunolabeling cells and tissues. I thank you for the support you have given.

I agree with your posting almost entirely and hope that we might have made our point by now. I have a few extra points to make which will probably end this thread for good (mostly from boredom, I guess).

First, I think we both agree that we should have reliable immunoreagents. I have preferences in the reagents I use, but I know there are other choices available. Use whatever works.

You express a reluctance to using cell fractions, something that someone off-line also disagreed with. I am not saying use poor morphology to get a result, I suggest using the best morphology that gives the result. In some instances the use of purified fractions of organelles has provided important results.
Perhaps the number of specialized organelles in a cell system is so low that it is impossible to find sufficient profiles of them in the thin sections we use. If we can positively identify these organelles by some other unique marker, then it is a simple task to section the fraction and apply two antibodies (one being the identifier, one being the experimental). This gives the information we look for and thus allow us to continue, hopefully to label sections of whole cells at a later date. This is just part of the data collection process for the whole project. Again, use what works.

I was being negative about secondary marker antibodies to add a little spice to my posting. I know that many people use these markers but I did want them to question their reliability. There is a tendency to condemn the primary antibody without thinking there may be other causes for signal to be absent. Maybe there is a need for careful monitoring of the gold probes during storage.

I do not know of one published work that has extensively examined the effect of storage on colloidal gold probes (same antigen system, same preparation protocols, antibody-gold compared with protein A-gold, systematic counting of gold particles etc.). If anyone knows of such a study, I would be interested to hear of it. I often use unconjugated secondary antibodies as a bridge for protein A-binding. It is clear that this extra step increases sensitivity and also adds more gold particles to the section (supported by published results from Jan Slot, I think). Although the gold is not so closely associated with the structures I expect to label, I still get results from this sort of labeling.

The problems I have with the use of pre-embedding techniques are the same I had when I first used them in the 1980's (and my morphology was pretty good in those days too). It was always difficult to quantify the result because I could never be completely sure that all the antigens had been totally accessible to the antibodies or visualization reagents. This problem may not be due to size of the particles used, or with permiabilization (which do play a role), but may also be due to other factors that cannot be controlled for, such as biological activity within whole mounts.
I am also reluctant to use pre-embedding labeling as a routine protocol because I cannot yet see a way to easily perform multiple labeling experiments. I look forward to being enlightened.

The possible lack of total accessibilty will also make it difficult to prove a negative result (how can I be sure that the vesicle lumen not labeled is because the antigen is not there, and not because the antibody or gold probe is not there?). I know these things are also problems with on-section labeling, but the methods for on section labeling are a little better understood. For this problem I would prepare thinner sections or count the gold particles. It may not improve things but I would be sure there is some possibility for labeling to occur. Limitations are easier to live with when we understand them a little more. Having siad all that, I realize that for some systems, pre-embedding is the way to go. If it works, use it.

I strongly support your advice that we should let the result drive our work and accept that what we find may not fit in with the favorite theory of the lab. The protocols, reagents and equipment available to us for the study of morphology and immunocytochemistry are the most sophisticated we have ever had. For this reason we have the ability to provide completely unambiguous quantitative results that are difficult to ignore. Learning to stand by them is our next step.

Finally, in an attempt to spice things up and to keep this thread going in some form, here are some extra comments to consider. Remember my question many years ago on this listserver when I asked if EM was a science or an art? All of the inital replies were that EM is an art. Only when I asked why we published in scientific journals did I get a few slightly more scientific replies. They didn't convince me too much though.
I still think that many EM people see themselves as artists able to perform wonderful things (i.e. miracles) with complicated machines. We are wrong to think this. We are also wrong to think that we are able to provide every researcher who walks through the door with the result they need to finish their grant/paper/report. With the tools available, we can provide unbiased results that will have biological meaning only in the context of what else is known about the system. For immunocytochemical experiments at least, we should really be active participants in the whole project. Non-microscopists need to read this stuff and think about what they expect from EM too.

Best regards,

Paul Webster.


Paul Webster, Ph.D.
Associate Scientist & Director
Ahmanson Advanced Electron Microscopy & Imaging Center
House Ear Institute
2100 West Third St.
Los Angeles, CA 90057

Phone: (213) 273-8026
Fax: (213) 413-6739
e-mail: pwebster-at-hei.org
http://www.hei.org/htm/aemi.htm




From daemon Thu Sep 28 18:38:07 2000



From: Jaclynn Lett :      jlett-at-cid.wustl.edu
Date: Thu, 28 Sep 2000 18:23:42 -0500
Subject: Conference: New Frontiers in the Amelioration of Hearing Loss

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CENTRAL INSTITUTE FOR THE DEAF, St. Louis, Missouri


announces the following:




New Frontiers in the Amelioration of Hearing Loss


A Conference in Honor of James D. Miller


CID is now accepting poster submissions and registration for this
conference in St. Louis, MO, March 22-25, 2001.


The conference will offer two expert seminar tracks, one for cellular and
molecular biologists and one for scientists studying applied technologies
and methods for the rehabilitation of children and adults with hearing
loss. The speakers include some of the most active and progressive
researchers working today.


In addition to serving as a forum for cutting-edge research, the conference
is designed to bridge the information gap between basic and applied
research related to hearing and deafness. Scientists from different tracks
will be provided with helpful information during a primer session held
before each expert seminar track. Social sessions will bring together
diverse professionals to foster new perspectives and ideas. Attendees will
have the opportunity to tour new CID campus facilities, including the Fay
and Carl Simons Center for Biology of Hearing and Deafness and CID's new
"quiet" oral school.


For poster guidelines, preliminary program and registration materials,
please contact Sarah Uffman, CID Research Department, 4560 Clayton Avenue,
St. Louis, MO 63110, or visit our web site at www.cid.wustl.edu
{ {http://www.cid.wustl.edu/} http://www.cid.wustl.edu/} .



Jaclynn M. Lett, Research Assistant


Faye and Carl Simons Center for the Biology of Hearing and Deafness
Central Institute for the Deaf
4560 Clayton Avenue
St. Louis, MO 63110





From daemon Thu Sep 28 19:58:23 2000



From: Schibler, Matthew :      MSchibler-at-mednet.ucla.edu
Date: Thu, 28 Sep 2000 17:45:02 -0700
Subject: Cell synchronisation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ken,

Mitosis is quite short, so if you get the cells reasonably synchronized
(they pass through mitosis in a Gaussian distribution), you can probably
catch most if not all stages if you time your collection right.

See our paper in Methods in Enzymology for a synchronization protocol:

Brady, R.C, M.J. Schibler and F. Cabral. 1986. Isolation of mitotic spindles
from mammalian cells. Meth. Enzymol. 134: 217-225.


Matthew J. Schibler Ph.D.
UCLA Brain Research Institute
1524A Gonda (Goldschmied) Center
for Neuroscience and Genetics
Los Angeles, CA 90095-1761

(310) 825-9783
FAX (310) 206-5855
E-mail: mschibler-at-mednet.ucla.edu


-----Original Message-----
} From: ken blight [mailto:blight-at-icrf.icnet.uk]
Sent: Thursday, September 28, 2000 8:16 AM
To: EM information site


I am interested in synchronising Hela cells and collecting samples in each
stage of Mitosis. Does anybody have a protocol and the times at which each
stage is reached after release from G1?

Ken Blight
Senior Scientific Officer
Electron Microscopy Unit
Imperial Cancer Research Fund
London
England





From daemon Fri Sep 29 02:51:03 2000



From: Claudia Hayward-Costa :      LS_S562-at-crystal.kingston.ac.uk
Date: Fri, 29 Sep 2000 08:35:10 +0100
Subject: Re: TEM/SEM bacteria protocol

Contents Retrieved from Microscopy Listserver Archives
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Dear David,

The answer I gave Kristine was purely theoretically - because I read
about it.

Unfortunately our CPD is out of service since years and so far I
have not used HMDS because I still have a few mls of PELDRI II.

Sorry, I can't give you an answer.

Regards

Claudia




Dr. C. Hayward-Costa
School of Life Sciences
Kingston University
Penrhyn Road, Kingston upon Thames
Surrey KT1 2EE, UK
44(0)208 547 2000 x 2240
Email: c.hayward-at-kingston.ac.uk



From daemon Fri Sep 29 08:30:56 2000



From: Anthony Garratt-Reed :      tonygr-at-mit.edu
Date: Fri, 29 Sep 2000 09:15:39 -0400
Subject: Microtome

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


My apologies for using network bandwidth for this, but I can't possibly
respond to all the e-mail and phone messages individually. However, the
microtome I offered yesterday has already found a good home where it will
be productively used by students. Many thanks to all of you who responded.

Tony.



** Anthony J. Garratt-Reed
** MIT Room # 13-1027
** 77 Massachusetts Avenue
** Cambridge, MA 02139-4307
** USA
**
** Phone: (+) 1-617-253-4622
** Fax: (+) 1-617-258-6478
**





From daemon Fri Sep 29 10:51:21 2000



From: Margaret Brannigan :      brannign-at-asrr.arsusda.gov
Date: Fri, 29 Sep 2000 11:31:40 -0400
Subject: Glycerol fixation/plant tissue/LM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

Some colleagues are trying to fix plant tissue for LM and possibly
confocal microscopy using ETOH and acetic acid--they asked me if I
knew what % of glycerol to include to keep the samples from being too
brittle. Does anyone know a good protocol for glycerol fixation?
They don't want to go the glutaraldehyde &/or osmium route. I
remember seeing something about glycerol fixation in an ancient book
on plant microtechnique but the book disappeared long ago...

Thanks for bailing me out again,
Margaret
--
Margaret Dienelt
Plant Pathologist

Electron Microscopy Lab
FNPRU, National Arboretum
ARS, USDA

B. 010A R. 238 BARC-W
10300 Baltimore Avenue
Beltsville, MD. 20705

Telephone: (301) 504-6097
Fax: (301) 504-5096



From daemon Fri Sep 29 10:54:15 2000



From: Lucille A. Giannuzzi :      lag-at-mail.ucf.edu
Date: Fri, 29 Sep 2000 11:37:46 -0400
Subject: Re: salaries for staff

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thanks to all who replied to my query on staff salaries.

To summarize the findings: The ranges that I reported for industry are
similar for university facilities, although the universities tend to be in
the lower end of each range. The people in higher cost of living regions
don't seem to be making that much more than those living in other regions.
The actualy salary offerings vary with duties required and experience (as
they should), so one should not use just the numbers to compare salaries.

The (sad) bottom line is: Administrators (the person(s) above the lab
director) think that staff salaries are adequate.

Thanks,
Lucille

*******************************************************************
Lucille A. Giannuzzi, Ph.D.

Associate Professor, Mechanical, Materials, and Aerospace Engineering
Director, UCF/Cirent Materials Characterization Facility
University of Central Florida
12443 Research Parkway, Suite 305
Orlando, FL 32826 email lag-at-mail.ucf.edu phone (407) 275-4354,5,6
fax (407) 275-4321
--------------------------------------------------------------------
"Good judgement comes from experience.

Experience comes from making bad judgement."

Mark Twain
********************************************************************





From daemon Fri Sep 29 13:23:05 2000



From: Betty Loraamm :      loraamm-at-chuma.cas.usf.edu
Date: Fri, 29 Sep 2000 14:03:23 -0400
Subject: LKB Huxley Part

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am looking for a replacement part for an LKB Huxley ultramicrotome Mark 2.
Does anyone know of an LKB Dealer or possibly have in their lab a spare
Suspension Strip Hinge (LKB Part No. W52271-30)? This piece is made of
polypropylene rod with 2 thin indented areas in the rod.




From daemon Fri Sep 29 13:24:53 2000



From: Hao Li :      haoli-at-glue.umd.edu
Date: Fri, 29 Sep 2000 14:17:28 -0400
Subject: cleavage of MgO

Contents Retrieved from Microscopy Listserver Archives
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Thanks a lot for all the responses. However, it seems that not everbody has
the same opinion regarding the possibility of cleaving samples with MgO
substrate for x-section TEM. Does anybody have sucessful experices on this?
Thanks a lot.

Hao Li




From daemon Fri Sep 29 13:45:56 2000



From: Robert Mixon :      mixonr-at-ohsu.edu
Date: Fri, 29 Sep 2000 11:32:40 -0700
Subject: Re: Glycerol fixation/plant tissue/LM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Margaret: 2 -3% glycerol should be just fine.

} } } Margaret Brannigan {brannign-at-asrr.arsusda.gov} 09/29 8:31 AM } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Hi,

Some colleagues are trying to fix plant tissue for LM and possibly
confocal microscopy using ETOH and acetic acid--they asked me if I
knew what % of glycerol to include to keep the samples from being too
brittle. Does anyone know a good protocol for glycerol fixation?
They don't want to go the glutaraldehyde &/or osmium route. I
remember seeing something about glycerol fixation in an ancient book
on plant microtechnique but the book disappeared long ago...

Thanks for bailing me out again,
Margaret
--
Margaret Dienelt
Plant Pathologist

Electron Microscopy Lab
FNPRU, National Arboretum
ARS, USDA

B. 010A R. 238 BARC-W
10300 Baltimore Avenue
Beltsville, MD. 20705

Telephone: (301) 504-6097
Fax: (301) 504-5096





From daemon Fri Sep 29 13:52:37 2000



From: Pauline C. Yu :      splene-at-pw.usda.gov
Date: Fri, 29 Sep 2000 11:39:34 -0700 (PDT)
Subject: Re: Glycerol fixation/plant tissue/LM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On Fri, 29 Sep 2000, Margaret Brannigan wrote:
} Hi,
}
} Some colleagues are trying to fix plant tissue for LM and possibly
} confocal microscopy using ETOH and acetic acid--they asked me if I
} knew what % of glycerol to include to keep the samples from being too
} brittle. Does anyone know a good protocol for glycerol fixation?

Steve Ruzin's Plant Microtechnique book gives a brief mention of softening
dried specimens in "10% glycerin-ethanol-water; probably 10% glycerol in
50% EtOH" and gives the reference as Peterson et al, 1989(Comparative leaf
anatomy of the annual Muhlenbergia. Nord. j. Bot. 8:575-583)

Pauline Yu
Microscopist Technician
USDA-ARS-WRRC




From daemon Fri Sep 29 15:27:56 2000



From: Lucille A. Giannuzzi :      lag-at-mail.ucf.edu
Date: Fri, 29 Sep 2000 16:09:59 -0400
Subject: staff salary responses

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Lots of people have asked me for results of the survey. My initial numbers
below were obtained from an industry (not university) inquery. These
numbers are higher than what my staff is actually receiving: (an I am
therefore trying to fix this).

$30's-40's: for starting engineers with little to no experience that
require training
mid $40's to mid $50's: for an experienced engineer
mid $50's to mid $70's: for a very skilled engineer who can trouble shoot
to the component level independently without any back-up help.

Summary of Responses: (I have removed any reference to individual names of
people/locations, etc.)

Based on what I have seen, and as reported by the ACS, your ranges are quite
acceptable.

} From what I have deduced from my commercial EM engineers, I think you're
right on the money. We don't have in house engineers that do this kind of
servicing. The in house guys get the middle range that you listed.

Your figures are pretty much on the mark. What you might
consider is how technicians (not engineers) fit into the
picture. They can be at pay scales $10K-$20K less overall yet
still do a very good job. Plus, they tend to stay on the premises
much longer than engineers, who seem to seek greater
rewards more frequently.

Your numbers look good to me, though I would add supervisory
responsibilities and lab management for someone in the upper range.

I am employed at XXX and my job is to service the electron
microscopes on campus. I repair at the component level and have not required
manufacturer help. I have many instruments
I am responsible for. I worked 11+ years for YYY in service and have
been here over 4. My salary is low 50's. Even though my job does not require
me to do so I work on other things as well. I put a new motor in a print
processor today. I often work on microtomes. I know I saved a department
about 6K a couple weeks ago by replacing a component in their power supply.
After inspection ZZZ would have sold them a new power supply. I fixed a
Horse treadmill last year. I love it here. A whole lot of fun.

These salaries seem to be in line (maybe a little higher) with what
universities pay technical persons doing these kinds of jobs. In my case, I
reluctantly transitioned from industry after 19 years and went from 70's to
low 40's. My observation is this, if you are trying to find good people with
experience then most likely you would need to offer salaries near the upper
end of these ranges. Keep in mind that technology changes, and even
experienced people need training and interaction with vendors. I believe
this investment will pay off as you are able in time to eliminate expensive
service contracts on all instruments. Salary is just part of the package
that will attract good people. I believe a good technical person will
flourish in the academic environment if they have a good salary, are free
from benefit concerns, and have the resources to do the job well.

As a coincidence, one of my technologists pulled your request for survey
response off the web to tell me that I was under paying him. I presume that
your classification of "engineer" refers to technical degree level people. I
manage a group of 6 technical people with about ten state of the art level
SEM's, TEM, EMP's and XRD equipment for a very long term contract.
Because we reside near Silicon Valley we must reach deeper for starting
rates here where entry
level housing is about $300K. We cannot compete with the high tech firms
that offer stock options, signing bonuses, etc. I just lost a materials
scientist who about doubled her salary at a firm in Fremont. Obviously, our
starting rates are a little higher than you indicate for
engineers/scientists as well as technicians with little difference for
degree status. I don't think that we have started anyone in our
organization lower than the mid $40's in the past couple of years.
Otherwise, we work with a similar salary range for non senior level
professionals without significant supervisory responsibility.

We have service contracts on all machines so much of our staff is
engaged in teaching etc.. and minor maintenance. In any case the $s sound
about the same for us as well... except the third category for which we do
not have anyone. But benefits (health, 403b, facilities etc..) are of great
interest to most which varies so widely across institutions

I get 65K, and probably should get
a hell-of-alot more. But then again,
I am such a push-over.

In our Characterization Facility most of the
user training and class teaching is done by PhD staffmembers with the title
"research associate" or "senior research associate" (both faculty rank,
non-tenure track positions). At many universities the analogous staff are
called "assistant research professor" or "associate research professor".
Some training is done by "junior" or "senior" level "scientists" with
bachelors or masters degrees; these are "civil service" staff and members of
the state workers union. Our instrument service and some administrative
tasks are done by the above people, or by our technician, altogether ranging
from bachelors to PhD degrees in various sciences (materials science,
physics, chemistry, geology). I'm not sure how this translates to your
"staff engineers". Our pay ranges have always struck me as pitifully low,
relative to what our
staff could make in analogous positions in industry. Here they are: PhD
staff: low $40k's to low $60k's including very experienced people
throughout the range. bachelors to masters staff: low $30k's to high $40k's.
All of these people are capable of independent troubleshooting. Most,
however, are not electrical or mechanical engineers. Thus much of our
service is contracted to the instrument companies. We are funded almost
entirely by our revenue, so down time is very detrimental and in many cases
it makes no sense to try to service things ourselves at the level of the
guts of electronics. Of course we do our own troubleshooting to identify the
problematic instrument component, but in most cases we do not service things
in the sense of an electrical engineer. No doubt it is difficult to make
comparisons given the diversity of
scenarios at the various universities plus industry. I applaud you for
attempting.

we have three people who work in the areas you have discussed. They train
students and faculty to use the instruments, and they maintain eleven
microscopes and sample prep equipment. They also perform administrative
tasks. They all have more than 15 years experience with microscopes. They
are in the $50K to $60K salary range. Because of their many years of
service and the high quality of their work, I consider their pay to be at
least 15% lower than it should be. If possible, I would be interested in
the results of the other responses.





From daemon Fri Sep 29 16:03:36 2000



From: Betty Loraamm :      loraamm-at-chuma.cas.usf.edu
Date: Fri, 29 Sep 2000 16:45:19 -0400
Subject: LKB Huxley Part

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am looking for a replacement part for an LKB Huxley ultramicrotome
Mark2. Does anyone know of an LKB Dealer or possibly have in their lab
a spare Suspension Strip Hinge (LKB Part No. W52271-30)? This piece is
made of polypropylene rod with 2 thin indented areas in the rod.

Betty Loraamm
Univ. of South Florida
Dept. of Biology SCA 110
4202 E. Fowler Ave
Tampa, FL 33620-5150
(813)974-2676
Fax:(813)974-3263




From daemon Fri Sep 29 17:51:29 2000



From: Ekstrom, Harry :      harry.ekstrom-at-honeywell.com
Date: Fri, 29 Sep 2000 15:33:00 -0700
Subject: Bench Top Turbo

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


My posting to this list server from a few weeks ago stating our problems
with the Denton Bench Top Turbo may have been taken out of context. Denton
is resolving our problems with the carbon coater. There customer service
has been excellent. I want to offer my apologies for any misrepresentation
I may have caused them.


Harry Ekstrom
Materials Laboratory
(602) 231-2744
e-mail: harry.ekstrom-at-honeywell.com




From daemon Fri Sep 29 18:41:44 2000



From: Norman_C_Miller-at-res.raytheon.com
Date: Fri, 29 Sep 2000 18:26:02 -0500
Subject: quantitative WDS analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


All,

I have been carrying our quantitative analysis of a lead titanate like oxide
using a Microspec WDX-2A-3PC on a scanning electron microscope. I have modified
the normal experimental procedure and the reproducability improved from 3-5% to
1%. Are there any further modifications of experimental procedure that might
further improve the reproducability by another factor of 2?

I am using a compound standard similar to all of the unknowns. All samples are
polished well. Count times are long enough to get statistics well better
than 1%
for each of the 5 elements. Data is collected from each element on standard and
then on sample, and thus beam current in the SEM is stable to better than 1%.

I am talking about reproducability of the k ratios being accurate to better
than
1%, not about the accuracy of the measured composition. I think the compound
standard will produce the best accuracy one can do.

any suggestions would be very welcome.'

N. Carl Miller





From daemon Sat Sep 30 12:27:08 2000



From: Thomas Litzinger :      thomas_c_litzinger-at-yahoo.com
Date: Sat, 30 Sep 2000 10:11:19 -0700 (PDT)
Subject: SEM protocol for Drosophila eye cross-section

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello everyone,
I am an undergraduate at Miami University, and
could use some protocol advise to best achieve the
results I am looking for.
I have already obtained a protocol for general
drosohpila SEM sample preparation, but my specific
area of interest is the eye. I was wondering what the
best method for acquiring a clean cross-section would
be. If you have done this, or could give me some
helpful hints it would be greatly appreciated.
Thanks,
Thomas Litzinger

__________________________________________________
Do You Yahoo!?
Yahoo! Photos - 35mm Quality Prints, Now Get 15 Free!
http://photos.yahoo.com/






From daemon Sun Oct 1 01:04:14 2000



From: Gary Gaugler :      gary-at-gaugler.com
Date: Sat, 30 Sep 2000 22:40:24 -0700
Subject: Coolpix 990 to Axioskop

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Has anyone tried interfacing the Zeiss Axioskop 1
to Nikon CoolPix 990 with success? How did you
do it? Were the results good?

Anyone tried the Fuji FinePix S1 Pro yet with
success?

gary g




From daemon Sun Oct 1 12:56:51 2000



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Mon, 2 Oct 2000 06:41:46 GMT+1200
Subject: Platinum Wire

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi

Can anybody suggest a supplier from whom I can buy some 0.001"
diameter Pt wire?
Please include e-mail address if possible.

thanks

rtch


Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand



From daemon Tue Oct 3 00:37:05 2000



From: Jason Verie :      dont8-at-planet-mail.com
Date: Mon, 02 Oct 2000 21:26:07 -0500
Subject: Your Cards #7DF0

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Accepting credit cards for your business
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************************************************************
If you receive this message and have never joined one of our
email lists you can be removed by replying to:
mailto:derr9-at-goplay.com?subject=remove
************************************************************





From daemon Mon Sep 4 09:08:10 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Wed, 4 Oct 2000 09:07:12 -0500
Subject: Testing 123

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html







From daemon Wed Oct 4 09:12:29 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Wed, 4 Oct 2000 08:52:59 -0500
Subject: Administrivia: Server should be back on-line

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Colleagues....

Sorry all but it looks like I screwed up again. Over the weekend
I was editing the filtering software trying to increase the
SPAM/Junk Mail rejection capabilities..... Well, I manaaged to stop
all mail to the List which means I was 100% effective
in filtering the Junk Mail!! ;-)

In any event, I think things are back on track, and working again.
If you tried to post anything between Oct 1 and today
you should consider it as being permanently lost in the Ether..

Just resubmit them...

BTW, thanks to all those people who started sending me Email
saying " I haven't gotten any mail in the last few days" it started
me looking for problems. I guess it's good to know you all miss
your Daily Microscopy Email-Fix. Almost sounds addictive.
(Hmmm... too bad I can't bottle it an make a few $$)

Cheers..

Nestor
Your Friendly Neighborhood SysOp.






From daemon Wed Oct 4 10:08:00 2000



From: s_woodland-at-madasafish.com
Date: Wed, 4 Oct 2000 10:04:39 -0500
Subject: Ask-A-Microscopist: Diatoms

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


---------------------------------------------------------------------------

Email: s_woodland-at-madasafish.com
Name: Simon Woodland
School: Kaskenmoor School

Question: Where can I get some diatoms to put under our microscope? How
should they be prepared and mounted.

---------------------------------------------------------------------------





From daemon Wed Oct 4 10:34:46 2000



From: Geoff Williams :      willi1gl-at-cmich.edu
Date: Wed, 04 Oct 2000 11:30:39 -0400
Subject: Flexible TLC prep for SEM?

Contents Retrieved from Microscopy Listserver Archives
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I have an application for separating various size particles using Thin
Layer Chromatography.
Question, has anyone prepped the polyester backing, with silica gel
coating for SEM?

I have a couple hypothetical protocols I could try but I thought I would
send this question out to the masses, to see if anyone has had
experience examining TLC plates in the SEM (regluar SEM not an ESEM or
VPSEM).

Thanks in Advance,
Geoff Williams

Electron Microscope Facility Supervisor
Biology Department
Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859

Geoffrey.Lloyd.Williams-at-cmich.edu
517 774-3576
517 774-3462 (fax)





From daemon Wed Oct 4 11:06:11 2000



From: John J. Bozzola :      bozzola-at-siu.edu
Date: Wed, 4 Oct 2000 11:02:47 -0500
Subject: SEM: ISI HV Power supply (resending)

Contents Retrieved from Microscopy Listserver Archives
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A colleague on campus is having a problem with their ISI Alpha 9 SEM.

They have diagnosed a failure of the high voltage power supply.
Specifically, the gun side of the multi-tap power supply has been
fried.

So, they are looking for the power supply for the Alpha 9 or an ISI
Mini SEM. If anyone has such a component (or even the whole SEM) for
sale or donation, please contact me.

Thank you.

John B.

####################################################################
John J. Bozzola, Ph.D., Director
IMAGE (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
####################################################################



From daemon Wed Oct 4 11:14:58 2000



From: Fortner, Jeffrey A. :      fortner-at-cmt.anl.gov
Date: Wed, 4 Oct 2000 11:11:36 -0500
Subject: TEM position open

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} The Chemical Technology Division of Argonne National Laboratory has an
} opening for a staff microscopist to perform transmission electron
} microscopy in support of the nuclear fuel cycle, waste management, and
} other programs as required. This person will also develop new processes
} and/or equipment and other technology to support the programs, and
} interface with sponsors to maintain existing support and develop new
} initiatives.
}
} This position requires a Ph.D. in chemistry, materials science, or
} related discipline and 2-5 years of experience (or equivalent).
}
} For further information or to send resume, contact David Chamberlain by
} electronic mail : chamberlain-at-cmt.anl.gov.
}
}



From daemon Wed Oct 4 12:21:49 2000



From: NVo-at-IGC.com
Date: Wed, 4 Oct 2000 13:15:58 -0400
Subject: Ge vs Si detector

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listners:

Any comments on which would make a better detector, Ge or Si ? only PGT
sells Ge detectors and I'm aware of the decrease in resolution with Ge
detector. Could someone lists or details the benefits of having a Ge
detector as oppose to a Si one.

Sincerely

N.V.Vo



N.V.Vo, Ph.D.
Intermagnetics General Corporation
450 Duane Avenue
Schenectady, New York 12304
Phone: 518 346 1414 x 3107
Fax: 518 346 6080
E-mail: nvo-at-igc.com
www.igc.com




From daemon Wed Oct 4 13:02:41 2000



From: Sharon Coe :      slc6-at-lehigh.edu
Date: Wed, 4 Oct 2000 13:10:48 -0500
Subject: Faculty recruitment at Lehigh

Contents Retrieved from Microscopy Listserver Archives
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Simon
I am not a diatom expert, but I know someone who is. Put your
question to Professor David Mann at the Royal Botanic Garden
Edinburgh. He eats, sleeps and breathes diatoms (maybe I
exaggerate a little!). His email address is
d.mann-at-rbge.org.uk

good hunting
Chris Jeffree

} From: "s_woodland-at-madasafish.com"-at-sparc5.microscopy.com
Date sent: Wed, 4 Oct 2000 10:04:39 -0500
To: Microscopy-at-sparc5.microscopy.com


Faculty Position
Department of Materials Science and Engineering
LEHIGH UNIVERSITY

Lehigh seeks to fill a tenure-track position, at the level of associate
or assistant professor, in Materials Science and Engineering. Research
interests in any area of materials will be considered but the person
selected will have a major commitment to the use of electron microscopy.
The Materials Department at Lehigh has research strengths in areas of
ceramics, metals, polymers and electronic materials. Details can be
found from the faculty pages at the departmental web site:
http://www.lehigh.edu/~inmatsci/inmatsci.html. It will be considered an
advantage if the research interests of the applicant are related to
existing research interests in such a way that collaborative work is
promoted. Lehigh runs an outstanding electron microscopy facility.
Favorable consideration will be given to candidates whose research will
involve substantial use of electron microscopy, especially when the
microscopy is innovative rather than routine. Lehigh is committed to
recruiting, retaining and tenuring women and members of minority groups.
Please submit an application by December 15, 2000, to Sharon Coe,
Department of Materials Science and Engineering, Lehigh University, 5
East Packer Avenue, Bethlehem PA 18015-3195, USA (slc6-at-lehigh.edu). To
discuss the post contact Alwyn Eades (jae5-at-lehigh.edu) at the same address.

==================================================================
Dr. Nestor J. Zaluzec
Materials Science Division
Building 212
Argonne National Lab
9700 S. Cass Ave
Argonne, Illinois 60439 USA
Tel: 630-252-7901, Fax: 630-252-4798
Email: Zaluzec-at-aaem.amc.anl.gov
==================================================================
TPMLab: http://tpm.amc.anl.gov
MMSite: http://www.amc.anl.gov
==================================================================

The box said "This program requires Win 95/98/NT or better..." so I bought
a G3 Mac

==================================================================





From daemon Wed Oct 4 13:40:06 2000



From: Stuart McKernan :      mckernan-at-cems.umn.edu
Date: Wed, 4 Oct 2000 13:28:51 -0500 (CDT)
Subject: MMS October Meeting

Contents Retrieved from Microscopy Listserver Archives
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The Minnesota Microscopy Society will be holding it's annual Fall Kick-Off event
on Thursday, October 12, 2000

This will follow the traditional format of a dinner followed by a talk, which
this year is:

"Nature's Jewels and Scientific Tools: Confessions of a Career Diatomist"

by Mark B. Edlund from the Science museum of Minnesota.


Abstract:

Diatoms are single-celled or colonial micro-organisms that possess a cell wall
of ornamented opaline silica (biologically-produced glass) and are commonly
referred to as the golden-brown algae. It is the wall ornamentation and shape
that are used to identify the nearly 25,000 species of living diatoms. Diatoms
were "discovered" within the first hundred years of microscopy; Leeuwenhoek
himself may be credited with one of the earliest illustrations (1703). Since
that time, diatoms have been the jewels, the postage stamps, and the tools of
amateur and professional diatomists. Microscopical societies burgeoned in the
latter half of the 19th century with members actively exchanging diatomaceous
material, slides, and methods and discussing publications and exsicattae.
Diatoms figured strongly in the development of light microscopy as test
organisms for resolving power; even today, many microscopists have an
Amphipleura pellucida slide in the drawer. Transmission electron microscopy
revealed much-needed knowledge on the cytology and mechanism of cell wall
formation in diatoms, but it has been the scanning electron microscope that has
truly changed the field. Ultrastructural details seen in the SEM coupled with
"rediscovery" of beautiful cytological work done in the late 1800's and early
1900's radically changed our modern understanding of diatom systematics. Lastly,
diatoms have proved to be excellent indicators of water quality and
environmental change. Whereas descriptive and biodiversity studies are still
common, applied methods now dominate the current scientific efforts.

Speaker: Mark B. Edlund, Research Scientist
Science Museum of Minnesota

Mark Edlund has a PhD in Natural Resources from the University of Michigan, and
has been specializing in diatoms for the last ten years. Besides field work in
the United States, he has done work at Lake Baikal in Siberia, and recently in
Mongolia.

Program:
5:30-6:00 PM Social hour
6:00-7:00 PM Dinner
7:00-7:15 PM Business meeting
7:15-8:15 PM Speaker

During the social hour, wine, cheese and crackers will be served.
The cost of the meal is $20 per person.

Location:
Cherrywood Room, 2nd Floor, St. Paul Students Cente,r St Paul Campus, University
of Minnesota, 2017 Buford Ave., St. Paul

Make Your Reservations Today

An accurate head count for the dinner is an absolute necessity. Contact Stuart
McKernan by Friday, October 6, to make your reservation (612-624-6009, or
stuartm-at-tc.umn.edu).




__________________
Stuart McKernan stuartm-at-tc.umn.edu
Office:(612) 626-7594
IT Characterization Facility, University of Minnesota Desk:(612) 624-6009
100 Union Street S. E., Minneapolis, MN 55455 NEW-} Fax:(612) 625-5368




From daemon Wed Oct 4 14:21:52 2000



From: White, Woody N. :      nwwhite-at-mcdermott.com
Date: Wed, 4 Oct 2000 14:17:25 -0500
Subject: Re: Ask a Microscopist - Diatomes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have not tried this source, but have heard:

You might try a swimming pool supply store. Look for Diatomaceous Earth
filter media. As to preparation... Techniques will depend on the type of
microscopy employed. If using a high vacuum electron microscope, an
electrically conductive film will need to be applied to the diatoms which
have been (typically) sprinkled onto double faced tape. The tape will not
have to be conductive (if it is - so much the better) if the whole mount is
conductively coated.

Woody White
McDermott Technology, Inc.
http://www.mtiresearch.com/

Me: http://home.att.net/~woody.white



From daemon Wed Oct 4 14:50:34 2000



From: Phillip Rutledge :      prutledge-at-ars.usda.gov
Date: Wed, 04 Oct 2000 13:43:21 -0600
Subject: messages

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Is there a problem with the server? I haven't received any messages in the last couple of days. I checked with our server and everything seems to be O.K.
Did my address get taken off by accident? In case it was my address is: prutledge-at-ars.usda.gov
Thanks,

Phillip Rutledge
USDA/ARS
Aquatic Animal Research Lab.
151 Dixon Drive
Chestertown, MD 21620
voice: 410.778.4136 or 2120
fax: 410.778.4399
e-mail: prutledge-at-ars.usda.gov



From daemon Wed Oct 4 14:50:34 2000



From: Wil Bigelow :      bigelow-at-engin.umich.edu
Date: Wed, 4 Oct 2000 14:50:43 -0400
Subject: RE: Pt wire

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I would try the two following companies as possible sources of fine
platinum wire:

GoodFellow, P.O. Box 628, Doylkestown, PA 18901-9975
FAX: 1-800-283-2020

Refining Systems Inc., P. O. Box 72446, Las Vegas, NV 89170
FAX: 702-368-0933

Both sell high purity and specialty metals in a variety of sizes and shapes.
Good luck,
WCB

Wilbur C. Bigelow, Prof. Emeritus
Materials Sci. & Engr., University of Michigan
Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu;
Fx:734-763-4788; Ph:734-662-5237





From daemon Wed Oct 4 14:51:53 2000



From: Leona Cohen-Gould :      lcgould-at-mail.med.cornell.edu
Date: Wed, 4 Oct 2000 15:50:00 -0400
Subject: Re: Ask-A-Microscopist: Diatoms

Contents Retrieved from Microscopy Listserver Archives
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At 10:04 AM -0500 10/4/00,
"s_woodland-at-madasafish.com"-at-sparc5.microscopy.com wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Have you tried Carolina Biological? They have all kinds of pre-mounted
slides for sale.

Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175





From daemon Wed Oct 4 16:35:14 2000



From: William Carmichael :      wcarmichael-at-madison.tec.wi.us
Date: Wed, 04 Oct 2000 16:31:03 -0500
Subject: powder TEM diffraction sample prep

Contents Retrieved from Microscopy Listserver Archives
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Anyone know of a easy, simple way to produce nice powder diffraction samples for illustrating the powder diffraction method for EM students? We use the International Centre for Diffraction Data For student identification of unknown samples. I have tried several methods that usually result in non-electron transparent samples (our TEM KV max is only 120). Any ideas?


_______________________________

Bill Carmichael
Electron Microscopy Faculty

Madison Area Technical College
3550 Anderson St.
Madison, WI 53704
608-243-4309

wcarmichael-at-madison.tec.wi.us
http://electron-microscopy.madison.tec.wi.us





From daemon Wed Oct 4 16:58:33 2000



From: Jeff Thole :      thole-at-macalester.edu
Date: Wed, 04 Oct 2000 16:56:27 -0500
Subject: Electronics/Electron Microscopy Position at Macalester College

Contents Retrieved from Microscopy Listserver Archives
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Macalester College (St. Paul, Minnesota) has a job opening which involves
looking for someone with a microscopy background (as well as electronics
skills). The job description is posted at:
http://www.macalester.edu/~hr/jobs/job551.html

The description is quite brief, but Macalester has both a Zeiss EM109 TEM
and a Zeiss DSM960A SEM. Maintenance and instruction for students/faculty
on these machines will be one aspect of this position.

Regards,

Jeff Thole



__________________________________________________

Jeff Thole - Geology Laboratory Supervisor and Instructor
Geology Department, Macalester College
1600 Grand Avenue, St. Paul, MN 55105
(ph. 651-696-6426, fax. x6122, email thole-at-macalester.edu)
__________________________________________________





From daemon Wed Oct 4 17:31:11 2000



From: COURYHOUSE-at-aol.com
Date: Wed, 4 Oct 2000 18:27:49 EDT
Subject: Re: Ask a Microscopist - Diatomes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Yes, indeed! You can buy a sack of the stuff there that will keep you busy
for all of eternity!

Also check with your neighbors where you live if they have pools and one of
them might spare a handful!

Ed Sharpe archivist for SMECC

{ { Subj: Re: Ask a Microscopist - Diatomes
Date: 10/4/00 3:20:58 PM US Mountain Standard Time
From: nwwhite-at-mcdermott.com (White, Woody N.)
To: woodland-at-madasafish.com ('woodland-at-madasafish.com'),
Microscopy-at-sparc5.microscopy.com ('Microscopy-at-sparc5.microscopy.com')

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America
To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
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-----------------------------------------------------------------------.


I have not tried this source, but have heard:

You might try a swimming pool supply store. Look for Diatomaceous Earth
filter media. As to preparation... Techniques will depend on the type of
microscopy employed. If using a high vacuum electron microscope, an
electrically conductive film will need to be applied to the diatoms which
have been (typically) sprinkled onto double faced tape. The tape will not
have to be conductive (if it is - so much the better) if the whole mount is
conductively coated.

Woody White
McDermott Technology, Inc.
http://www.mtiresearch.com/

Me: http://home.att.net/~woody.white

} }



From daemon Wed Oct 4 18:51:30 2000



From: Walck, Scott D. :      walck-at-ppg.com
Date: Wed, 4 Oct 2000 19:47:15 -0400
Subject: RE: powder TEM diffraction sample prep

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Take an oxide powder, grind it up with mortar and pestle and take a carbon
coated grid and swipe it across the fines. You can also use two glass
slides to grind the powder up.

You can evaporate a number of materials onto a carbon coated grid or onto a
cleaved NaCl sample and then float that off on water onto a grid.

You could smoke a Mo wire in air to provide crystals if you leave it in the
smoke long enough, you will probably have enough to cover the grid. Heat
the wire across the terminals of an evaporator or heat with a torch to
generate the white smoke. You could also burn some Mg and get MgO crystals.

If you don't have sufficient particles in a single diffraction pattern to
give you rings, take multiple exposures from different areas on the same
piece of film.

Incidentally, the MoO3 samples will provide you with a rotation calibration
sample.


-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)



} -----Original Message-----
} From: William Carmichael [mailto:wcarmichael-at-madison.tec.wi.us]
} Sent: Wednesday, October 04, 2000 5:31 PM
} To: microscopy-at-sparc5.microscopy.com
} Subject: powder TEM diffraction sample prep
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} ---------.
}
}
} Anyone know of a easy, simple way to produce nice powder
} diffraction samples for illustrating the powder diffraction
} method for EM students? We use the International Centre for
} Diffraction Data For student identification of unknown
} samples. I have tried several methods that usually result in
} non-electron transparent samples (our TEM KV max is only
} 120). Any ideas?
}
}
} _______________________________
}
} Bill Carmichael
} Electron Microscopy Faculty
}
} Madison Area Technical College
} 3550 Anderson St.
} Madison, WI 53704
} 608-243-4309
}
} wcarmichael-at-madison.tec.wi.us
} http://electron-microscopy.madison.tec.wi.us
}
}
}



From daemon Wed Oct 4 19:15:31 2000



From: Gary Gaugler :      gary-at-gaugler.com
Date: Wed, 04 Oct 2000 17:34:21 -0700
Subject: Re: Ask a Microscopist - Diatomes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Message-ID: {39DBE9C8.7597-at-netrax.net}


What is the difference between diatoms and radiolaria (Actinopods)?
They "look" the same to me.

gary g.


At 12:17 PM 10/4/00, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From daemon Wed Oct 4 19:52:33 2000



From: Earl Weltmer :      earlw-at-pacbell.net
Date: Wed, 04 Oct 2000 17:23:25 -0700
Subject: Bio Rad Rep in US

Contents Retrieved from Microscopy Listserver Archives
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Hi all,

I have a BioRad SC500 coater.
Can anyone give me the local US rep for service?

Thank You,

Earl Weltmer




From daemon Wed Oct 4 19:56:28 2000



From: Caroline Schooley :      schooley-at-mcn.org
Date: Wed, 4 Oct 2000 17:53:50 -0700
Subject: Re: Ask-A-Microscopist: Diatoms

Contents Retrieved from Microscopy Listserver Archives
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} ---------------------------------------------------------------------------
}
} Email: s_woodland-at-madasafish.com
} Name: Simon Woodland
} School: Kaskenmoor School
}
} Question: Where can I get some diatoms to put under our microscope? How
} should they be prepared and mounted.
}
} ---------------------------------------------------------------------------

Simon -

You can go to a big home supply store and ask for "diatomaceous earth";
it's used for a lot of filtering applications. You'll get a big sack, so
you can use it up by ordering the Lawrence Hall of Science/GEMS manual
"River Cutters" (see the Prtoject MICRO URL below), which uses the same
materiel to make a model stream bed. Or you can order a prepared slide
from a variety of suppliers, Or you can contact Joe Neilly
{joe.p.neilly-at-abbott.com} , keeper of Project MICRO's sandpile, and ask for
samples of foraminefera sand or the very exotic "star sand". Preparation?
Minimal. "Microscopic Explorations", MSA's GEMS manual, will tell you how
to make filecard and tape slides that will work well.

Caroline

Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html





From daemon Wed Oct 4 21:10:29 2000



From: Rosemary White :      Rosemary.White-at-pi.csiro.au
Date: Thu, 5 Oct 2000 13:07:54 +1000
Subject: Ask-A-Microscopist: Diatoms

Contents Retrieved from Microscopy Listserver Archives
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The source I have used in the past is kitty litter. It usually has a lot
of diatomaceous earth in it.

have fun,


Rosemary White
Microscopy Centre
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia

phone 61-2-6246 5475
fax 61-2-6246 5000
email r.white-at-pi.csiro.au





From daemon Wed Oct 4 21:55:54 2000



From: Ben Craft :      bcraft-at-uci.edu
Date: Wed, 4 Oct 2000 19:52:12 -0700 (PDT)
Subject: High Resolution noise

Contents Retrieved from Microscopy Listserver Archives
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I'm trying to solve a high resolution noise problem with a XL-30
Phillips FESEM. The room that it is in was recently checked and was ok,
accept for some acoustic noise. To fix the noise I put up 3 inch sound
proofing foam and quieter ducting for the air condition. The noise
showed up in an image by a streak about 5 jaggies. After
putting up the foam the noise was reduced, but it is still there. The
noise is the same for 4mm working distance or a 10mm working distance.

Any one had a similar problem or have any advice?

Thanks,
Ben




#######
#####\_O -Ben Craft-
####/\/}
#### /"
### \






From daemon Wed Oct 4 22:20:58 2000



From: Philip Oshel :      peoshel-at-facstaff.wisc.edu
Date: Wed, 4 Oct 2000 22:22:38 -0500
Subject: Re: Ask a Microscopist - Diatomes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Gary,

There's a *lot* of difference. About that between a Redwood and an
Elephant. Or more. And as distinctive.

Diatoms are about as good a plant as can be found among the
protistans. They look like more-or-less fancy silicate pillboxes with
lots of little holes.

Radiolaria are amoeboid beasts with a complicated silicate internal
"skeleton". They tend to be nested, holey spheres, usually with a
more-or-less complex profusion of spikes, needles, and the like.

Crude descriptions, but brief. The best way to see the difference is
to get a copy of Ernst Haeckel's book published by Dover -- "Arts
Forms in Nature". Beautiful drawings of many different organisms,
including Radiolaria and Diatoms.

Phil

} What is the difference between diatoms and radiolaria (Actinopods)?
} They "look" the same to me.
}
} gary g.
}
--
*** Be famous! Send a Tech Tip or article to Microscopy Today! ***
Philip Oshel
Technical Editor, Microscopy Today
P.O. Box 620068
Middleton, WI 53562-0068
Voice: days (608) 263-4162, evening (608) 833-2885
Fax (608) 836-1969 (please make sure my name is on any fax)

Address for UPS, FedEx, or other couriers:
Dept. of Animal Health and Biomedical Sciences
University of Wisconsin
1656 Linden Drive
Madison, WI 53706-1581
fax: (608) 262-7420 (dept. fax)

peoshel-at-facstaff.wisc.edu



From daemon Wed Oct 4 22:56:49 2000



From: Vitaly Feingold :      vitalylazar-at-worldnet.att.net
Date: Wed, 4 Oct 2000 23:54:09 -0400
Subject: Fw: TEM calibration standard

Contents Retrieved from Microscopy Listserver Archives
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Dear listers,

What certified (NIST traceable?) TEM mag. calibration samples are available
for 20K to over 100K range? Diffraction grating replica sample will not
provide sufficient accuracy- too few grating periods "fit" in the image at
higher magnifications.

Vitaly Feingold
Scientific Instruments and Applications
2773 Heath Lane, Duluth GA 30096
(770)232-7785 ph.
(770)232-1791 fax


This message is made of 100% recycled electrons.




From daemon Wed Oct 4 23:20:11 2000



From: Purdy, Sam :      SPurdy-at-nationalsteel.com
Date: Wed, 4 Oct 2000 23:16:09 -0500
Subject: : Ask-A-Microscopist: Diatoms

Contents Retrieved from Microscopy Listserver Archives
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Simon:

Try Wards Natural History Establishment in upstate New York. The
exact address escapes me.

Sam Purdy
TechnicalCenter
National Steel Corp
Trenton MI


} ----------
} From: "s_woodland-at-madasafish.com"-at-sparc5.microscopy.com
} Sent: 4, October 2000, 11:04 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Ask-A-Microscopist: Diatoms
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} --------------------------------------------------------------------------
} -
}
} Email: s_woodland-at-madasafish.com
} Name: Simon Woodland
} School: Kaskenmoor School
}
} Question: Where can I get some diatoms to put under our microscope? How
} should they be prepared and mounted.
}
} --------------------------------------------------------------------------
} -
}
}
}





From daemon Thu Oct 5 00:37:14 2000



From: Anton Gutakovskii :      gut-at-thermo.isp.nsc.ru
Date: Thu, 5 Oct 2000 12:35:59 +0600
Subject: help for jem-4000

Contents Retrieved from Microscopy Listserver Archives
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Dear Listers!

We have the problems with our JEM-4000EX: the ion pump SIP-1 is not OK!
The possible causes are:
1/- leak in vacuum system (after bake out of the SIP);
2/-some contamination into SIP (this SIP was go OK more 40,000 hour);
3/- ???
Does anyone know how to make testing of this situation?

How do to demount the pump, what order of actions?

I am not currently part of the list, so please mail to me directly.

--
Best regards,
Anton Gutakovskii mailto:gut-at-thermo.isp.nsc.ru





From daemon Thu Oct 5 03:25:38 2000



From: PETER HEIMANN :      peter.heimann-at-biologie.uni-bielefeld.de
Date: Thu, 05 Oct 2000 10:21:37 +0200
Subject: Re: Coolpix 990 to Axioskop (or any other photomicroscope) "re-sent"

Contents Retrieved from Microscopy Listserver Archives
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Yes,
mounting the Coolpix 990 to any photomicroscope is absolutely
simple via the usual C-mount connection. For the coolpix you have
to buy at NIKON the microscope adapter ( here in germany this
adapter is around 1000.- DM or approx. 450.- US $; adapter is not
listed in Nikon catalogue; contact the microscope section of Nikon)
fitting on one side to the thread at the front of the lens and on the
other side to your microscope-specific C-mount adapter ( c-mount
adapter for Zeiss: buy the more expensive one which has the
possibility of correction of the focus plane).
The fantastic thing is that you can focus not only via the LCD-
screen of the camera ( possible, but not so easy in my opinion) but
via an external TV-Monitor in live modus, yielding really smashing
results with a resolution satisfying publication needs.
With my Coolpix sometimes the camera "hangs up" when the Auto-
power off mode is activated (de-activating it helps). Does anybody
have this problem as well ?? ( Contact me off-line).
What is lacking for the Coolpix 990 at the moment is a cable
release, but the Nikon people here in germany tell me it will be
available at the end of the year (probably very expensive).

All in all, the Coolpix is the first digital camera under 5000 US-$ I
have used or tested which is fully satisfying me in my daily lab
routine (photomicroscope (even fluorescence), binocular, makro-photographs)
especially due to its simple and clear operation, the possibility to
adjust everything (speed, aperture, focus, etc.) manually
and its excellent results.

Peter Heimann

**************************************************
please reply to: peter.heimann-at-biologie.uni-bielefeld.de ("E-MAIL")

Dr. Peter Heimann
Developmental Biology & Molecular Pathology; W7-107
University of Bielefeld
D 33501 Bielefeld / Germany
phone: xx49(0)521-106-5628 / 5627
FAX : xx49(0)521-106-5654
WEB-Site: http://www.uni-bielefeld.de/biologie/Entwicklungsbiologie
WEB-Site: http://www.uni-bielefeld.de/SFB549
******************************************************



From daemon Thu Oct 5 03:56:15 2000



From: Kevin Mackenzie :      nhi691-at-abdn.ac.uk
Date: Thu, 5 Oct 2000 09:55:41 +0100 (BST)
Subject: Scottish Microscopy meeting

Contents Retrieved from Microscopy Listserver Archives
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28th SCOTTISH MICROSCOPY SYMPOSIUM

http://www.abdn.ac.uk/emunit/smg2000.htm

Wednesday 15 November 2000, Moredun Institute, Edinburgh.

The annual symposium is supported by all area groups in Scotland.
The programme for this meeting is comprised of the following three
invited talks together with seven contributed talks on a wide
range of microscopy with general appeal and a poster display.
As in recent years, a comprehensive trade exhibition by leading companies
will be present. The Trade Exhibition, Poster Display and Catering
will all be together in one central area of this excellent venue.

15/11/2000 Programme

Multi-photon microscopy. Professor Alison Gurney, Dept.of
Physiology and Pharmacology, University of Strathclyde.

Ultrastructure eggshell quality assessment: a way forward for
captive breeding programmes. Avril Edmond, Dept.
Veterinary Anatomy, Glasgow.

Non-invasive imaging of plant tissues using the confocal laser
scanning microscope (CLSM) Dr. Karl Oparka, Unit of Cell Biology, SCRI,
Dundee.

Functional dynamics of plasmodesmata in sink/source transition
leaves. Alison Roberts, Unit of Cell Biology, SCRI, Dundee

COSMIC - a new interdisciplinary facility for advanced
spectroscopy, micromanipulation and imaging. Professor Wilson
Poon, Dept. of Physics and Astronomy, University of Edinburgh.

Imaging intracellular location of psychoactive drugs
using fluorescence microscopy. Richard Horobin, IBLS, Glasgow.

Scanning infra-red microscopy. Alison Coats, Dept. Chemistry,
Aberdeen University.

Antigen retrieval allows improved visualisation of V-ATPase
immuno-reactivity. Susan Lindsay, Caledonian University, Glasgow.

Techniques in Immunocytochemistry. Peter Jackson, Research
Histology Unit, Dept. of Histopathology and Molecular Pathology,
Leeds General Infirmary. Talk sponsored by DACO Ltd.


See web site for abstracts from previous meetings

http://www.abdn.ac.uk/emunit/smg99.htm

Kevin Mackenzie
Electron Microscope unit
Dept Zoology
University of Aberdeen
Tillydrone avenue
Aberdeen
AB24 2TZ
-----------------
Tel 01224 272847
Fax 01224 272396
email k.s.mackenzie-at-abdn.ac.uk






From daemon Thu Oct 5 04:26:41 2000



From: patrick gunning :      patrick.gunning-at-bbsrc.ac.uk
Date: Thu, 5 Oct 2000 10:16:18 +0100
Subject: Atomic Force Microscopy for Biologists

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I hope this isn't seen as an overt advert on the list server, I just want to
let people know that this new book is available and hope it may be of
general interest. Rest assured that I and my fellow co-authors get very
little of the sales proceeds!

A.Patrick Gunning
IFR Norwich

ATOMIC FORCE MICROSCOPY FOR BIOLOGISTS

by V J Morris, A R Kirby & A P Gunning

Atomic force microscopy (AFM) is part of a range of emerging microscopic
methods for biologists which offer the magnification range of both the light
and electron microscope, but allow imaging under the 'natural' conditions
usually associated with the light microscope. To biologists AFM offers the
prospect of high resolution images of biological material, images of
molecules and their interactions even under physiological conditions, and
the study of molecular processes in living systems. This book provides a
realistic appreciation of the advantages and limitations of the technique
and the present and future potential for improving the understanding of
biological systems.

Contents:
Apparatus
Basic Principles
Macromolecules
Interfacial Systems
Ordered Macromolecules
Cells, Tissue and Biominerals
Other Probe Microscopes

Readership: Undergraduates, postgraduates and researchers in biophysics.
352pp Pub. date: Dec 1999 Imperial College Press
ISBN 1-86094-199-0 US$51 / £32



From daemon Thu Oct 5 05:34:44 2000



From: Bart Cannon :      cannonmp-at-accessone.com
Date: Thu, 05 Oct 2000 03:36:26 -0700
Subject: Re: Coolpix 990 to Axioskop (or any other photomicroscope) "re-sent"

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Peter,

I think one can use the "self timer" function of the Coolpix 990 to
expose an image without touching the camera body at the time of the
exposure.

I can think of no engineering reason why the cable release would need to
be expensive when it becomes available. I'll bet any electronics
tinkerer could make one for $30.

I'm quite happy with my Cool-Pix 990. If only I could remember 10% of
its function modes and how to access them.

Bart Cannon
Cannon Microprobe
Seattle




From daemon Thu Oct 5 07:14:30 2000



From: Jinguo Wang :      jqw11-at-psu.edu
Date: Thu, 05 Oct 2000 08:37:36 -0400
Subject: Enlarger or digital image system

Contents Retrieved from Microscopy Listserver Archives
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High-purity Germanium detectors provide *better* resolution than
Si(Li) detectors, and also have lower low-keV background. The
higher atomic number of Ge means they have greater x-ray
stopping power and therefore higher detection efficiency for the K
lines of the heavier elements, and they have therefore been thought
of as primarily useful at higher beam accelerating voltages for
heavy element analysis. However, this may only be one of their
advantages. The better resolution and lower background they offer
at low keV suggests they would also be good, and perhaps
preferable to Si for light element detection and peak discrimination
which could mean more options for low-kV excitation in Field
emission SEMs.

What are the flaws in this argument?
It would be interesting to hear comments on the above from anyone
who has direct knowledge and experience of both types of
detectors.

BTW besides PGT, both Gresham and Oxford sell Ge detectors. I
am not aware of any others
Chris



} From: "NVo-at-IGC.com"-at-sparc5.microscopy.com
To: Microscopy-at-sparc5.microscopy.com


Dear Listers:

I am trying to find an enlarger and digital image system to print the
conventional and HREM negatives. What kind of enlarger and digital image
system are better? manufacturer? prices?

Thanks a lot!

Jinguo Wang





From daemon Thu Oct 5 08:03:00 2000



From: rgriffin-at-eng.uab.edu
Date: Thu, 5 Oct 2000 07:54:39 -0500
Subject: Digital microscope camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} I'm looking to replace my two year old Polaroid Digital Microscope camera
} (SCSI interface with color or black and white, 1600x1200 pixels). The
} chip has failed and I don't want to buy another Polaroid camera. I do
} primarily black and white photography from a Zeiss optical microscope for
} materials engineering applications. I need for the camera to save in tiff
} format without changing the dimensions of the objects. I don't need low
} light applications.
}
} Any suggestions?
}
} Thanks,
}
} Robin Griffin
}



From daemon Thu Oct 5 08:04:45 2000



From: Joseph Neilly :      joe.p.neilly-at-abbott.com
Date: Thu, 5 Oct 2000 08:04:03 -0500
Subject: Re: powder TEM diffraction sample prep

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Bill,

We have found a simple method for preparing drug particles for electron
diffraction. We suspend particles in a solvent by sonication for 2-3 seconds
and then let the suspension settle for 10-20 seconds. The largest, heavy
particles will settle first leaving the thin, light ones in suspension. Then
take a drop (20 ul) from the top of the solution, place it on a carbon filmed
grid and allow it to dry. The result is a distribution of particles laying
flat accross the grid. You need a solvent in which the particles are not
soluble. We typically use hexane.

Joe Neilly
Abbott Laboratories
D-45M, AP31
200 Abbott Park Rd.
Abbott Park, IL 60064-6202
voice: (847)-938-5024
fax: (847)-938-5027
e-mail: joe.neilly-at-abbott.com



From daemon Thu Oct 5 08:16:50 2000



From: Xu, Yuhui (NHLBI) :      XuY-at-nhlbi.nih.gov
Date: Thu, 5 Oct 2000 09:10:48 -0400
Subject: TEM of DNA and Protein complex

Contents Retrieved from Microscopy Listserver Archives
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Dear colleagues:

I have two questions on TEM of DNA and protein complex:
1. which method is the best , or most suitable ,to study such samples?
Direct spray to the mica sheet and subsequent metal rotary shadowing, or
cytochrome c spreading with metal coating examined by TEM, or atomic force
microscopy?
2. Is there any computer software which is designed specifically for analyze
these type of images?

Any comments will be greatly appreciated.

Yuhui

Yuhui Xu, MD,PhD
EM Core, NHLBI, NIH



From daemon Thu Oct 5 08:23:32 2000



From: Lou Solebello :      microls1297-at-mindspring.com
Date: Thu, 5 Oct 2000 08:20:29 -0500
Subject: XRD Wanted

Contents Retrieved from Microscopy Listserver Archives
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This is a bit off the topic of microscopy, but most of us on this
server do work in facilities that have XRD units. If anyone is selling,
or knows of someone who is selling their XRD, please contact me. I am also
curious if anyone has had any experience with the new desk tops like
Rigaku has. I plan on demoing one in the future. At $50k-$60k, they are
very cost efficient compared to standard XRD units, but I am wary of their
performance as an analytical instrument. Lou Solebello





From daemon Thu Oct 5 08:49:51 2000



From: Linton Brown :      l.j.brown-at-stir.ac.uk
Date: Thu, 5 Oct 2000 14:47:42 +0100
Subject: Protocol for fish egg samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear members,
I am a PhD student and I use a subscriber to find an answer to a problem I
have.
I am trying to find a method to fix and process whole fish eggs in order to
cut sections for immunohistochemistry. Any suggestions?

Ioannis Vatsos
Institute of Aquaculture
University of Stirling
Scotland, UK



From daemon Thu Oct 5 09:13:29 2000



From: Brian McIntyre :      mcintyre-at-optics.rochester.edu
Date: Thu, 05 Oct 2000 10:04:00 -0400
Subject: saw-tooth scan distortion: jeol35

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


hi all-

i've installed a 35 and have some issues with a saw-tooth distortion in the
scan. it stays constant regardless of KV, but the number of saw-tooths
(teeth?) in a frame increases with slower scan rates. i suspected
vibration so i mounted the roughing pumps on a big foam block.....no
change. i also cycled off the chiller unit with no change, so its probably
not mechanical vibrations, unless there is another vibration causing
component i'm overlooking.

the distortion is also visible in the data field alphanumerics (they shift
and shimmy a little) with the beam turned off, so i don't suspect a HV
instability problem.

can i logically deduce that there is an electrical field leakage that is
messing up the scan? or a ground loop? what about cable placement? or a
problem with the 100V variac (its within 3' of the column).

right now the column is a bit dirty and needs cleaning....i've never seen,
for example, dirty apertures cause this type of distortion though.

any suggestions would be appreciated!

brian
----------------------------------------------
Brian McIntyre
Electron Microscopy Lab- River Campus
Univ of Rochester
Rochester, NY 14627
716-275-3058/4875



From daemon Thu Oct 5 09:13:34 2000



From: Ladd Research :      ladres-at-worldnet.att.net
Date: Thu, 05 Oct 2000 10:09:41 -0400
Subject: Re: Bio Rad Rep in US

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Earl,

We distributed the Polaron coaters for years. We can assist in spare
parts and perhaps service.

John Arnott


--

LADD RESEARCH
131 Dorset Lane
Williston, VT 05495 USA

web site http://www.laddresearch.com

TEL 1-800-451-3406 (US) or 1-802-878-6711 (anywhere)
FAX 1-802-878-8074
e-mail sales-at-laddresearch.com

Microscope Supplies Since 1955



Earl Weltmer wrote:


} Hi all,
}
} I have a BioRad SC500 coater.
} Can anyone give me the local US rep for service?
}
} Thank You,
}
} Earl Weltmer



From daemon Thu Oct 5 09:24:19 2000



From: McCaffrey, John :      John.McCaffrey-at-nrc.ca
Date: Thu, 5 Oct 2000 10:19:28 -0400
Subject: Fw: TEM calibration standard

Contents Retrieved from Microscopy Listserver Archives
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Hello Vitaly,

There currently is no NIST-traceable standard for high-magnification
TEM on the market. The next best thing is the MAG*I*CAL TEM calibration
sample, which is internally calibrated against the lattice spacing of
silicon, a fundamental constant of nature. This statement has been used
successfully in the past in fulfillment of the requirements for
certification and traceability. The MAG*I*CAL sample allows magnification
calibrations across the entire magnification range of TEMs, as well as the
camera constant calibration and the image/diffraction pattern rotation
calibration.

The sample is marketed by South Bay Technology, TEL: 800-728-2233,
1120 Via Callejon, San Clemente, CA 92673 USA, e-mail:
henriks-at-southbaytech.com It is also marketed through various microscopy
supply houses, (SPI, EMS, Pelco, etc.) if you have a favorite.

Your request again raises the concern that the microscopy community
needs a high-magnification TEM calibration sample that is NIST-certified or
NIST-traceable. NIST is currently considering investigating the need for a
high-magnification TEM calibration reference, but will not proceed unless
there is some interest shown from the user community. It would be helpful
to the community in general if you could write a note or send an email to
Ms. Nancy Trahey, stating the problem that you have encountered, and
reiterating the need for such a NIST-certified or NIST-traceable standard.
Her co-ordinates are listed below.

Ms. Nancy M. Trahey
Standard Reference Materials Program (232)
Engineering Mechanics (202), Room 113
NIST
100 Bureau Drive, Stop 2320
Gaithersburg, MD 20899-2320
Tel: (301) 975-2021
email: nancy.trahey-at-nist.gov

Disclaimer: I 'invented' the MAG*I*CAL TEM calibration sample and retain
all bragging rights; it is listed in the Guinness Book of Records as "The
World's Smallest Ruler".


John P. McCaffrey, Ph.D.
National Research Council of Canada
M-50, Montreal Rd.
Ottawa, Ontario
K1A 0R6 CANADA

tel: +613-993-7823
fax: +613-990-0202
email: john.mccaffrey-at-nrc.ca


-----Original Message-----
} From: Vitaly Feingold [mailto:vitalylazar-at-worldnet.att.net]
Sent: Wednesday, October 04, 2000 11:54 PM
To: .


Dear listers,

What certified (NIST traceable?) TEM mag. calibration samples are available
for 20K to over 100K range? Diffraction grating replica sample will not
provide sufficient accuracy- too few grating periods "fit" in the image at
higher magnifications.

Vitaly Feingold
Scientific Instruments and Applications
2773 Heath Lane, Duluth GA 30096
(770)232-7785 ph.
(770)232-1791 fax


This message is made of 100% recycled electrons.




From daemon Thu Oct 5 09:33:42 2000



From: michael shaffer :      epmalab-at-darkwing.uoregon.edu
Date: Thu, 5 Oct 2000 07:21:11 -0700
Subject: RE: Ge vs Si detector

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


N.V.Vo writes ...

} Any comments on which would make a better detector, Ge or
} Si ? only PGT sells Ge detectors and I'm aware of
} the decrease in resolution with Ge detector.
} Could someone lists or details the benefits of having a Ge
} detector as oppose to a Si one.

We are using a "Gem" detector from Oxford Instruments with
absoloutely no problems ... 115eV resolution, fast count rates, and
very little energy shift with varying time constants. Another benefit
is there are no escape peaks for x-ray lines 0~10keV. One drawback
may be if you intend to allow the detector to come up to ambient
temperatures at times. Si detectors seem to accommodate this, whereas
it was suggested at the time of purchase we absolutely not allow this
.. but I believe that was simply because the "Gem" was for the most
part untested in this respect. I have no idea of this detector's
ambient T survivability, we have always kept it at LN2 T.

cheerios, =shAf= :o)

{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {}
Michael Shaffer, R.A. - mshaf-at-darkwing.uoregon.edu
Geological Science's Electron Probe Facility - University of Oregon
http://epmalab.uoregon.edu/





From daemon Thu Oct 5 09:49:47 2000



From: carla_aiwohi-at-usgs.gov
Date: Thu, 5 Oct 2000 06:46:53 -0700
Subject: SEM Help needed on conductive adhesive

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello all,

I am attaching CP dried salmon skins (approx. 2-3 mm thick) to stubs with
Electrodag 502 for secondary electron imaging. The adhesive is a graphite
paint in MEK base (a substitute for TV Tube Koat). After ethanol
dehydration and CP drying in liquid CO2, the samples are relatively flat or
slightly curved. However, once attached to the stub the skins develop a
noticeable curl which leaves a gap between the stub surface and sample. In
some cases I can see the "trail" in the adhesive left by the skin as it was
curling! Filling the gap with more Electrodag is a waste of time because
it evaporates and shrinks back away from the sample (thankfully it doesn't
seem to produce more curling).

Could anyone suggest a better conductive adhesive? Also, is there any
difference between aqueous (eg. Aquadag colloidal graphite) and MEK-based
media as to its effect on biological samples?

As an SEM novice any advice you could share with me is greatly appreciated.
Thank you very much,

Carla Aiwohi
Western Fisheries Research Center
Seattle, WA
ph: (206) 526-6282 ext.242
fax: (206) 526-6654




From daemon Thu Oct 5 10:09:34 2000



From: William F. Tivol :      wft03-at-health.state.ny.us
Date: Thu, 5 Oct 2000 11:06:34 -0400
Subject: Re: powder TEM diffraction sample prep

Contents Retrieved from Microscopy Listserver Archives
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Anyone know of a easy, simple way to produce nice powder diffraction samples for
illustrating the powder diffraction method for EM students? We use the
International Centre for Diffraction Data For student identification of unknown
samples. I have tried several methods that usually result in non-electron
transparent samples (our TEM KV max is only 120). Any ideas?

Dear Bill,
The simplest way I know is to evaporate a layer of Au or Al onto a
formvar-covered grid. By spreading
the beam, you can get continuous rings, and by using a small selected area you
can get discrete spots at various positions around the rings, so you can
demonstrate directly that the rings consist of the summed intensities from many
crystalites of differing orientations. Good luck.
Yours,

Bill Tivol
Wadsworth Center
Albany NY
(518) 473-7399 WFT02-at-health.state.ny.us





From daemon Thu Oct 5 10:17:52 2000



From: JIM ROMANOW :      bsgphy3-at-uconnvm.uconn.edu
Date: Thu, 5 Oct 2000 11:16:34 -0400
Subject: High Resolution noise

Contents Retrieved from Microscopy Listserver Archives
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Ben,
Our Zeiss DSM982 FESEM is also sensitive to acoustic noise. "The quieter
the room the more noises we hear." There is no simple answer, just keep
trying different things. I was told one lab installed a switch to turn the
SEM PC cooling fans off for short periods of time during high resolution
work. That is a risky fix if you are over 50 years old.

We strategically padded all connections to the column and redirected the
room air flow but have not installed acoustic foam on the walls. Evenings
are quieter than days. At 300kx we can detect a backhoe 600 yards away.

Good Luck
Jim



} Date: Wed, 4 Oct 2000 19:52:12 -0700 (PDT)
} From: Ben Craft {bcraft-at-uci.edu}
} X-Sender: bcraft-at-taurus.oac.uci.edu
} To: Microscopy-at-sparc5.microscopy.com
} Subject: High Resolution noise
} MIME-Version: 1.0
} }
}
} I'm trying to solve a high resolution noise problem with a XL-30
} Phillips FESEM. The room that it is in was recently checked and was ok,
} accept for some acoustic noise. To fix the noise I put up 3 inch sound
} proofing foam and quieter ducting for the air condition. The noise
} showed up in an image by a streak about 5 jaggies. After
} putting up the foam the noise was reduced, but it is still there. The
} noise is the same for 4mm working distance or a 10mm working distance.
}
} Any one had a similar problem or have any advice?
}
} Thanks,
} Ben
}
}
}
}
} #######
} #####\_O -Ben Craft-
} ####/\/}
} #### /"
} ### \
}

James S. Romanow
The University of Connecticut
Physiology and Neurobiology Department
Electron Microscopy Facility
U-2131
Storrs, CT 06269-2131
bsgphy3-at-uconnvm.uconn.edu
860 486-2914 voice
860 486-1936 fax





From daemon Thu Oct 5 10:53:07 2000



From: Greg Strout :      gstrout-at-ou.edu
Date: Thu, 05 Oct 2000 10:49:06 -0500
Subject: Re: Ask-A-Microscopist: Diatoms

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Simon,

You could prepare your own. I find that the diatoms in diatomaceous earth are
usually broken and like to prepare my own. You will need a plankton net, some
potassium dichromate, and 30% peroxide. Once the diatoms are collected
(plankton net tows from shoreline) you can clean them using the 30% peroxide
and potassium dichromate. Place the diatoms in a large beaker (and I mean
large here) then pour in 15 to 20 mls of peroxide followed by a large pinch of
the potasium dichromate. The resultant reaction oxidizes organics leaving the
glass frustules of the diatoms unharmed. It is better to be cautious with the
amounts that you use as this is an exothermic reaction, sometimes violently
so. Not that you'll have an explosion, but I've had to clean up boil overs on
a number of occasions. Once the reaction subsides and the solution turns a
golden yellow color it is just a matter of washing the diatoms. There are a
couple of ways to do this. You could centrifuge the diatoms, then throw out
the liquid, add water, centrifuge, throw out the liquid, etc. or you could fill
the large beaker with water and let the diatoms settle to the bottom (1-2hrs),
carefully pour off the fluid, and add more water, let the diatoms settle, etc.
The diatoms will appear as a milky white residue in the bottom of your
container (you may have to concentrate the material to really see this). If
you need any more info please feel free to contact me.

Greg

"s_woodland-at-madasafish.com"-at-sparc5.microscopy.com wrote:

} ---------------------------------------------------------------------------
}
} Email: s_woodland-at-madasafish.com
} Name: Simon Woodland
} School: Kaskenmoor School
}
} Question: Where can I get some diatoms to put under our microscope? How
} should they be prepared and mounted.
}
} ---------------------------------------------------------------------------

--
==================================================================
Greg Strout
Electron Microscopist, University of Oklahoma
WWW Virtual Library for Microscopy:
http://www.ou.edu/research/electron/www-vl/
e-mail: gstrout-at-ou.edu
Opinions expressed herein are mine and not necessarily those of
the University of Oklahoma
==================================================================





From daemon Thu Oct 5 10:57:33 2000



From: jim quinn :      jquinn-at-doL1.eng.sunysb.edu
Date: Thu, 5 Oct 2000 11:51:05 -0400
Subject: Re: Coolpix 990 to Axioskop (or any other photomicroscope) "re-sent"

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Bart and Peter

Besides Nikon's own release (EU-1 wired remote control) for
the CoolPix990, you can purchase a third-party release cable
from the CKCpower.com website.

Jim


}
} Hi Peter,
}
} I think one can use the "self timer" function of the Coolpix 990 to
} expose an image without touching the camera body at the time of the
} exposure.
}
} I can think of no engineering reason why the cable release would need to
} be expensive when it becomes available. I'll bet any electronics
} tinkerer could make one for $30.
}
} I'm quite happy with my Cool-Pix 990. If only I could remember 10% of
} its function modes and how to access them.
}
} Bart Cannon
} Cannon Microprobe
} Seattle
}
}



From daemon Thu Oct 5 11:34:58 2000



From: Daniel Ross :      DKRoss-at-uh.edu
Date: Thu, 05 Oct 2000 11:35:00 -0500
Subject: Ge vs Si detectors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have a Ge detector and get considerably better resolution with it than we
get with an older SiLi detector. The Ge detector has an ultrathin window,
the Si detector has a Be window. They are both used for x-ray detection in
the 0-20 KeV range.

Kent Ross

-----------------------------------------
Kent Ross
Research Associate
Texas Center for Superconductivity
University of Houston
77204-5932
DKROSS-at-uh.edu
713-743-8284
------------------------------------------




From daemon Thu Oct 5 11:44:13 2000



From: michael shaffer :      epmalab-at-darkwing.uoregon.edu
Date: Thu, 5 Oct 2000 09:31:26 -0700
Subject: RE: Coolpix 990 to Axioskop (or any other photomicroscope) "re-sent"

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


PETER writes ...

} mounting the Coolpix 990 to any photomicroscope is absolutely
} simple via the usual C-mount connection. For the coolpix you have
} to buy at NIKON the microscope adapter ...
} The fantastic thing is that you can focus not only via the LCD-
} screen of the camera ( possible, but not so easy in my opinion) but
} via an external TV-Monitor in live modus, yielding really smashing
} results with a resolution satisfying publication needs.
} With my Coolpix sometimes the camera "hangs up" when the Auto-
} power off mode is activated (de-activating it helps). Does anybody
} have this problem as well ?? ...

The AC adapter should allow for the video output to remain on ...
leastwise, that is how my CP800 works.

} What is lacking for the Coolpix 990 at the moment is a cable
} release, ...
}
} All in all, the Coolpix is the first digital camera under
} 5000 US-$ ...

The 990 is indeed feature rich, and the cable release will be needed
for microscopy (... altho you should be able to acquire with a steady
camera if you take a picture with the time-delay feature ...).

I have realized just lately, altho the CP950 and 990 are the
feature-rich and most desireable, the CP800 will work (altho somewhat
awkwardly) ... providing the same NTSC/PAL out and lens threads. If
you find a lesser expensive 1x C-mount adapter (as available at Optem
International {www.optemintl.com} ), then you have a digital camera
capable of 1600by1200 for approximately US$800. You should figure an
additional $100 for AC adapter ($50) and several NiMH batteries and
charger (~$50).

cheerios, =shAf= :o)

{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {}
Michael Shaffer, R.A. - mshaf-at-darkwing.uoregon.edu
Geological Science's Electron Probe Facility - University of Oregon
http://epmalab.uoregon.edu/





From daemon Thu Oct 5 12:15:15 2000



From: Dee Breger :      micro-at-ldeo.columbia.edu
Date: Thu, 5 Oct 2000 13:10:21 -0400 (EDT)
Subject: Re: Ask-A-Microscopist: Diatoms

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Robin,

Have you considered or looked into the Sony 390P color video camera?? It
gives you a live image with an RGB output, C-mount and 1.3 million pixels
(effective resolution) and sells for just under $ 3,000.

If you would like any more information, please let me know.

Sincerely,

Gary

Gary Liechty
Manager, Technical Products

Allied High Tech Products, Inc.
2376 E. Pacifica Place
Rancho Dominguez, CA 90220

310-635-2466
800-675-1118 US and Canada
310-762-6808 Fax

Products for Metallurgical Sample Preparation

www.alliedhightech.com
----- Original Message -----
} From: {"rgriffin-at-eng.uab.edu"-at-sparc5.microscopy.com}
To: {microscopy-at-sparc5.microscopy.com}
Sent: Thursday, October 05, 2000 5:54 AM


Simon,

I suspect that diatomaceous earth will have mostly fragments in it. If you
let me know your address, I'll try to find an old diatom SEM preparation to
send you and I'll also ask some folks here for a fresh sample of whole
diatoms that you can prepare for viewing yourself.

Gary,

Being a microscopist, not a microbiologist/paleontologist/sedimentologist
(though I've imaged my share of microplankton for them) I can only give a
brief overview of the differences between diatoms and radiolaria, but here
it is. Rads are floating, single celled marine protozoa that live inside
ornate opaline tests (external shells) that look like little Christmas tree
ornaments or something you might play badminton with. Their shells have
lots of holes, spikes and spines and can be spherical, amorphously
latticed, rather bulbous with long spikes from one end, sub-triangular,
etc. The cell body inside sends out numerous fine cytoplasmic strands that
radiate outward to form a sticky halo that captures food. Diatoms are
single-celled plants that live wherever there's moisture - salt water,
fresh water, soil, even on whale skin. They can be planktonic
(free-floating) or benthic (attached to a substrate) or live on mud or
rocks. Some live in the dark, but most need light to photosynthesize.
Their siliceous tests are made of two valves that fit together like a pill
box and come in two basic shapes, round and pennate. (The pennate ones can
look like peanuts or canoes.) Diatom tests are also quite ornamental, with
holes, radial designs, or long, sometimes feathery, projections, and
sometimes they link together to form long chains. There's also a great
variety in size among species.

Dee


***************************************************************
Dee Breger
Mgr. SEM/EDX Facility
Lamont-Doherty Earth Observatory
61 Route 9W
Palisades, NY 10964 USA
T: 914/365-8640
F: 914/365-8155

http://www.ldeo.columbia.edu/micro
http://www.discovery.com/area/science/micro/micro1.html
http://www.lsc.org/antarctica/front.html
Journeys in Microspace (Columbia University Press, 1995)





From daemon Thu Oct 5 12:53:38 2000



From: Gary Radice :      gradice-at-richmond.edu
Date: Thu, 5 Oct 2000 13:47:32 -0400
Subject: re:CoolPix 990 to Axioscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


You might also try contacting Bunton Instruments in the US:

{http://www.buntgrp.com/}

They sell microscope adaptors for the CoolPix. The adaptors are not listed
on their web site, yet, but call or e-mail them for information. I know
they are taking orders for adaptors now and I was told that they would be
shipping "around the end of October."

I have no connection with Bunton Instruments other than as a customer.

Gary P. Radice gradice-at-richmond.edu
Associate Professor of Biology 804 289 8107 (voice)
University of Richmond 804 289 8233 (FAX)
Richmond VA 23173 http://www.science.richmond.edu/~radice





From daemon Thu Oct 5 12:54:03 2000



From: Anthony Garratt-Reed :      tonygr-at-mit.edu
Date: Thu, 05 Oct 2000 13:48:09 -0400
Subject: Re: saw-tooth scan distortion: jeol35

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Brian-

You don't mention at what magnifications you see the sawtooth. However, if
you see it in the alphanumerics, I can't see that it can be anything other
than a problem in your display unit - a 60Hz interference in the line scan
drive (but not the scan generator) or something like that. If that is the
case, the magnification wouldn't make any difference.

Good luck,

Tony Garratt-Reed

} hi all-
}
} i've installed a 35 and have some issues with a saw-tooth distortion in the
} scan. it stays constant regardless of KV, but the number of saw-tooths
} (teeth?) in a frame increases with slower scan rates. i suspected
} vibration so i mounted the roughing pumps on a big foam block.....no
} change. i also cycled off the chiller unit with no change, so its probably
} not mechanical vibrations, unless there is another vibration causing
} component i'm overlooking.
}
} the distortion is also visible in the data field alphanumerics (they shift
} and shimmy a little) with the beam turned off, so i don't suspect a HV
} instability problem.
}
} can i logically deduce that there is an electrical field leakage that is
} messing up the scan? or a ground loop? what about cable placement? or a
} problem with the 100V variac (its within 3' of the column).
}
} right now the column is a bit dirty and needs cleaning....i've never seen,
} for example, dirty apertures cause this type of distortion though.
}
} any suggestions would be appreciated!
}
} brian
} ----------------------------------------------
} Brian McIntyre
} Electron Microscopy Lab- River Campus
} Univ of Rochester
} Rochester, NY 14627
} 716-275-3058/4875
}

** Anthony J. Garratt-Reed
** MIT Room # 13-1027
** 77 Massachusetts Avenue
** Cambridge, MA 02139-4307
** USA
**
** Phone: (+) 1-617-253-4622
** Fax: (+) 1-617-258-6478
**





From daemon Thu Oct 5 13:25:18 2000



From: Becky Holdford :      r-holdford-at-ti.com
Date: Thu, 05 Oct 2000 13:30:28 -0500
Subject: Re: Ge vs Si detector

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We currently have 4 Ge EDX detectors and 1 Si(Li) detector, all from Oxford
Instruments. The Ge detectors do have a lower background than the Si(Li) and
the resolution is better. The Ge detectors range from 109 ev to 115 ev
whereas the Si(Li) is around 132 ev. We routinely use all of them for low-kV
work. The Ge detectors do a better job because of the resolution and the
lower background. I do SEM of semiconductors, so 30kV is as high as I can
go. I can't comment on the Ge detector's performance at higher than that, but
they are my favorite detector in the 0.5 kV to 30kV range.
N.B.: I have no financial interest in Oxford Instruments; I'm just a
satisfied customer.

Chris Jeffree wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} High-purity Germanium detectors provide *better* resolution than
} Si(Li) detectors, and also have lower low-keV background. The
} higher atomic number of Ge means they have greater x-ray
} stopping power and therefore higher detection efficiency for the K
} lines of the heavier elements, and they have therefore been thought
} of as primarily useful at higher beam accelerating voltages for
} heavy element analysis. However, this may only be one of their
} advantages. The better resolution and lower background they offer
} at low keV suggests they would also be good, and perhaps
} preferable to Si for light element detection and peak discrimination
} which could mean more options for low-kV excitation in Field
} emission SEMs.
}
} What are the flaws in this argument?
} It would be interesting to hear comments on the above from anyone
} who has direct knowledge and experience of both types of
} detectors.
}
} BTW besides PGT, both Gresham and Oxford sell Ge detectors. I
} am not aware of any others
} Chris
}
} } From: "NVo-at-IGC.com"-at-sparc5.microscopy.com
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Ge vs Si detector
} Date sent: Wed, 4 Oct 2000 13:15:58 -0400
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Dear Listners:
} }
} } Any comments on which would make a better detector, Ge or Si ? only PGT
} } sells Ge detectors and I'm aware of the decrease in resolution with Ge
} } detector. Could someone lists or details the benefits of having a Ge
} } detector as oppose to a Si one.
} }
} } Sincerely
} }
} } N.V.Vo
} }
} }
} }
} } N.V.Vo, Ph.D.
} } Intermagnetics General Corporation
} } 450 Duane Avenue
} } Schenectady, New York 12304
} } Phone: 518 346 1414 x 3107
} } Fax: 518 346 6080
} } E-mail: nvo-at-igc.com
} } www.igc.com
} }
} }
}
} ==========================================
} Dr. Chris Jeffree
} University of Edinburgh
} BIOSEM - Biological Sciences Electron Microscope Facility
} Institute of Cell and Molecular Biology
} Daniel Rutherford Building
} King's Buildings, Mayfield Road
} EDINBURGH, EH9 3JH, Scotland, UK
} Tel. #44 (0) 131 650 5345
} FAX. #44 (0) 131 650 6563
} Mobile 07710 585 401
} email c.jeffree-at-ed.ac.uk
} =========================================

--
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Becky Holdford (r-holdford-at-ti.com)
972-598-1291 (pager)
KFAB Physical Analysis Lab--SEM/FIB/FA
Texas Instruments, Inc.
Dallas, TX
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~





From daemon Thu Oct 5 14:11:59 2000



From: SMancuso-at-specialmetals.com
Date: Thu, 5 Oct 2000 15:08:07 -0400
Subject: Re: saw-tooth scan distortion: jeol35

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Brian,

Our 35C has several times displayed that saw-tooth interference in the
past.
Each time it was attributed to some ground loop problem. One place to look
is in the ribbon aperture. Make sure that the insulators between the
aperture
strip and holder are properly set or 'not missing'.

I agree that the distortion is not from HV instability which usually
displays some
darkness or brightness irregularity.

Good luck!
_
/ \ Sam O. Mancuso
/\ /\ Special Metals Corporation
(__n__) New Hartford, NY





Brian McIntyre
{mcintyre-at-optics.roch To: Microscopy-at-sparc5.microscopy.com
ester.edu} cc:
Subject: saw-tooth scan distortion: jeol35
10/05/2000 10:04 AM





------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


hi all-

i've installed a 35 and have some issues with a saw-tooth distortion in the
scan. it stays constant regardless of KV, but the number of saw-tooths
(teeth?) in a frame increases with slower scan rates. i suspected
vibration so i mounted the roughing pumps on a big foam block.....no
change. i also cycled off the chiller unit with no change, so its probably
not mechanical vibrations, unless there is another vibration causing
component i'm overlooking.

the distortion is also visible in the data field alphanumerics (they shift
and shimmy a little) with the beam turned off, so i don't suspect a HV
instability problem.

can i logically deduce that there is an electrical field leakage that is
messing up the scan? or a ground loop? what about cable placement? or a
problem with the 100V variac (its within 3' of the column).

right now the column is a bit dirty and needs cleaning....i've never seen,
for example, dirty apertures cause this type of distortion though.

any suggestions would be appreciated!

brian
----------------------------------------------
Brian McIntyre
Electron Microscopy Lab- River Campus
Univ of Rochester
Rochester, NY 14627
716-275-3058/4875








From daemon Thu Oct 5 15:02:09 2000



From: Schibler, Matthew :      MSchibler-at-mednet.ucla.edu
Date: Thu, 5 Oct 2000 12:54:53 -0700
Subject: Ask-A-Microscopist: Diatoms

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Carolina Biological Supply has diatoms for this purpose.
I just take them out of the jar with a dropper and make a wet mount with a
coverslip.

You can get them live or preserved.

www.carolina.com

Matthew J. Schibler Ph.D.
UCLA Brain Research Institute
1524A Gonda (Goldschmied) Center
for Neuroscience and Genetics
Los Angeles, CA 90095-1761

(310) 825-9783
FAX (310) 206-5855
E-mail: mschibler-at-mednet.ucla.edu


-----Original Message-----
} From: "s_woodland-at-madasafish.com"-at-sparc5.microscopy.com
[mailto:"s_woodland-at-madasafish.com"-at-sparc5.microscopy.com]
Sent: Wednesday, October 04, 2000 8:05 AM
To: Microscopy-at-sparc5.microscopy.com


---------------------------------------------------------------------------

Email: s_woodland-at-madasafish.com
Name: Simon Woodland
School: Kaskenmoor School

Question: Where can I get some diatoms to put under our microscope? How
should they be prepared and mounted.

---------------------------------------------------------------------------





From daemon Thu Oct 5 15:29:43 2000



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Fri, 6 Oct 2000 09:29:50 GMT+1200
Subject: Re: SEM Help needed on conductive adhesive

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



}
} I am attaching CP dried salmon skins (approx. 2-3 mm thick) to stubs with
} Electrodag 502 for secondary electron imaging. The adhesive is a graphite
} paint in MEK base (a substitute for TV Tube Koat). After ethanol
} dehydration and CP drying in liquid CO2, the samples are relatively flat or
} slightly curved. However, once attached to the stub the skins develop a
} noticeable curl which leaves a gap between the stub surface and sample. In
} some cases I can see the "trail" in the adhesive left by the skin as it was
} curling! Filling the gap with more Electrodag is a waste of time because
} it evaporates and shrinks back away from the sample (thankfully it doesn't
} seem to produce more curling).
}
} Could anyone suggest a better conductive adhesive? Also, is there any
} difference between aqueous (eg. Aquadag colloidal graphite) and MEK-based
} media as to its effect on biological samples?
}


I have found the conductive double-sided adhesive stuff available
from several EM suppliers in both disc and tape form to be pretty
good.
But maybe you can't press your samples down onto it.

cheers

rtch


Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand



From daemon Thu Oct 5 15:36:13 2000



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Fri, 6 Oct 2000 09:35:36 GMT+1200
Subject: Re: High Resolution noise

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html





} I was told one lab installed a switch to turn the
} SEM PC cooling fans off for short periods of time during high resolution
} work. That is a risky fix if you are over 50 years old.

??????????????????????????????

rtch


Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand



From daemon Thu Oct 5 16:21:30 2000



From: JIM ROMANOW :      bsgphy3-at-uconnvm.uconn.edu
Date: Thu, 5 Oct 2000 17:14:02 -0400
Subject: Re: High Resolution noise

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



}
}
} } I was told one lab installed a switch to turn the
} } SEM PC cooling fans off for short periods of time during high resolution
} } work. That is a risky fix if you are over 50 years old.
}
} ??????????????????????????????

Clarification:
I just turned 50. I if I attempted to turned these fans off manually for "a
few minutes", chances are the PC processor would shut down on overheat
before I remembered to turn them back on. It's an 'out of memory' problem.
Jim



}
} rtch
}
}
} Ritchie Sims Phone : 64 9 3737599 ext 7713
} Department of Geology Fax : 64 9 3737435
} The University of Auckland email : r.sims-at-auckland.ac.nz
} Private Bag 92019
} Auckland
} New Zealand
}

James S. Romanow
The University of Connecticut
Physiology and Neurobiology Department
Electron Microscopy Facility
U-2131
Storrs, CT 06269-2131
bsgphy3-at-uconnvm.uconn.edu
860 486-2914 voice
860 486-1936 fax





From daemon Thu Oct 5 16:47:31 2000



From: Vitaly Feingold :      vitalylazar-at-worldnet.att.net
Date: Thursday, October 05, 2000 10:20 AM
Subject: Re: Ge vs Si detector

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


EG&G ORTEC used to manufacture Germanium EDS detectors too. I do not know
whether they still do it since they had become part of Perkin-Elmer. They
have, however, a service group which does service Germanium detectors.

Vitaly Feingold
Scientific Instruments and Applications
2773 Heath Lane, Duluth GA 30096
(770)232-7785 ph.
(770)232-1791 fax

This message is made of 100% recycled electrons.
-----Original Message-----
} From: Chris Jeffree {cjeffree-at-srv0.bio.ed.ac.uk}
To: "NVo-at-IGC.com"-at-sparc5.microscopy.com
{"NVo-at-IGC.com"-at-sparc5.microscopy.com}
Cc: microscopy-at-sparc5.microscopy.com {microscopy-at-sparc5.microscopy.com}




From daemon Thu Oct 5 16:47:31 2000



From: Vitaly Feingold :      vitalylazar-at-worldnet.att.net
Date: Thursday, October 05, 2000 11:51 AM
Subject: saw-tooth scan distortion: jeol35

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Brian,

Information in your posting strongly suggests ground loop(s) and possibly EM
(electromagnetic) fields in the room too. It is possible for local EM
interference to affect signal cables without affecting the SEM column much.
Did you try EM shielding with sheets of soft Iron? If that makes no
difference (it probably wouldn't, since HT set makes no difference for the
distortion in your case), than some work needs to be done with
shielding/grounding of the various units/components of the SEM. Let us say
here, one who has the experience of eliminating 60Hz noise in audio circuits
can do the job.
Another possibility- did you check DC power supply voltages in your SEM for
60Hz noise? It must be filtered out well.

Vitaly Feingold
Scientific Instruments and Applications
2773 Heath Lane, Duluth GA 30096
(770)232-7785 ph.
(770)232-1791 fax

This message is made of 100% recycled electrons.
-----Original Message-----
} From: Brian McIntyre {mcintyre-at-optics.rochester.edu}
To: Microscopy-at-sparc5.microscopy.com {Microscopy-at-sparc5.microscopy.com}




From daemon Thu Oct 5 16:48:47 2000



From: Vitaly Feingold :      vitalylazar-at-worldnet.att.net
Date: Thu, 5 Oct 2000 17:33:12 -0400
Subject: TEM calibration standard

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thank you for all the responses - exactly what we looking for. MSA
listserver is at great help, as usual.

Vitaly Feingold
Scientific Instruments and Applications
2773 Heath Lane, Duluth GA 30096
(770)232-7785 ph.
(770)232-1791 fax

This message is made of 100% recycled electrons.




From daemon Thu Oct 5 17:55:01 2000



From: Mark West :      mwest-at-ifcsun1.ifisiol.unam.mx
Date: Thu, 5 Oct 2000 17:48:00 -0600 (=?ISO-8859-1?Q?Hora_est=E1ndar_de_M=E9xico?=)
Subject: Re: saw-tooth scan distortion: jeol35

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Brian,

your SEM interference problem sounds almost the same as ours that I not
long ago put on the listserver - the sawtooths in the scans. Sorry I
haven't got an answer to the problem, but I'd be very interested if you
manage to solve it! We are going to do a trial with mu-metal in the near
future shielding the column of our SEM (Jeol 5410-LV) so I'll let you know
how it goes. We suspect electromagnetic field interference.

Good luck,

Mark






********************************************
Mark West,
Unidad de Microscopia Electronica,
(Electron Microscopy Unit)
Instituto de Fisiologia Celular,
Universidad Nacional Autonoma de Mexico,
04510 Mexico D.F.

tel (unidad/lab) *(525) 622 5610*
(casa/home) (525) 619 3020
Fax (525) 616 2282
********************************************





From daemon Thu Oct 5 18:15:06 2000



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Thu, 05 Oct 2000 16:04:42 -0700
Subject: Re: Digital microscope camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Robin,
For your application, you may find the Pixera camera to be a cost-effective
solution. At the same resolutionoas your current camera, it costs about $1100.
At 07:54 AM 10/5/00 -0500, you wrote:

} } I'm looking to replace my two year old Polaroid Digital Microscope camera
} } (SCSI interface with color or black and white, 1600x1200 pixels). The
} } chip has failed and I don't want to buy another Polaroid camera. I do
} } primarily black and white photography from a Zeiss optical microscope for
} } materials engineering applications. I need for the camera to save in tiff
} } format without changing the dimensions of the objects. I don't need low
} } light applications.
} }
} } Any suggestions?
} }
} } Thanks,
} }
} } Robin Griffin
} }
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca




From daemon Thu Oct 5 18:27:41 2000



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Thu, 05 Oct 2000 16:17:54 -0700
Subject: Re: saw-tooth scan distortion: jeol35

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Brian,
You may have ripple on another power supply, such as lens or scan coils. I
always try to put the variac as far from the column as possible. Check for
something loose on the column or stage: I've had that effect when the gun HV
supply cable was not tied down. Make sure there is a good vibration break
(heavy weight) in the foreline hose. The service engineer at ours turned off
the rotary pump for a few seconds, to see if it changed. Another source of
vibration was a belt-drive pump in another room, but on the same floor beam.
A dirty column won't cause this. It may be building vibration.
At 10:04 AM 10/5/00 -0400, you wrote:
}
} hi all-
}
} i've installed a 35 and have some issues with a saw-tooth distortion in the
} scan. it stays constant regardless of KV, but the number of saw-tooths
} (teeth?) in a frame increases with slower scan rates. i suspected
} vibration so i mounted the roughing pumps on a big foam block.....no
} change. i also cycled off the chiller unit with no change, so its probably
} not mechanical vibrations, unless there is another vibration causing
} component i'm overlooking.
}
} the distortion is also visible in the data field alphanumerics (they shift
} and shimmy a little) with the beam turned off, so i don't suspect a HV
} instability problem.
}
} can i logically deduce that there is an electrical field leakage that is
} messing up the scan? or a ground loop? what about cable placement? or a
} problem with the 100V variac (its within 3' of the column).
}
} right now the column is a bit dirty and needs cleaning....i've never seen,
} for example, dirty apertures cause this type of distortion though.
}
} any suggestions would be appreciated!
}
} brian
Good luck!

Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca




From daemon Thu Oct 5 18:42:30 2000



From: Al Coritz :      al-at-boeckeler.com
Date: Thu, 5 Oct 2000 16:27:19 -0700
Subject: Ventana-RMC EM Products

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


October 6 , 2000


Dear Valued Customer,


We are pleased to inform you that Boeckeler Instruments, Inc., a local
Tucson high technology company, has acquired the RMC Electron Microscopy
product line from Ventana Medical Systems.

The RMC product management group including Dave Roberts, Greg Becker, Al
Coritz and Gareth Morgan will stay with the RMC product line and continue to
give you excellent support under the ownership of Boeckeler Instruments.

During the next few days we will be in the process of moving offices,
production lines and inventory so please accept our apologies if you
experience any difficulties or delays during this brief transitional period.
We will do our utmost to minimize any inconvenience to you by making this
move as “transparent” as possible to the outside world and we appreciate
your patience & continued support as we relocate the business.
Since 1942 Boeckeler has been and still is the industry leader in
manufacturing high quality and accurate micrometer and positioner heads.
Boeckeler Instruments also manufactures a complete line of video
measuring, cross hair and marking products all of which are very
complementary to the RMC ultramicrotomes and EM sample preparation
instruments.

We look forward to a long and continuing relationship with you as our highly
valued customer and hope to be of service to you in the very near future.

If you have any questions or concerns please contact:


Boeckeler Instruments, Inc.

4650 South Butterfield Drive

Tucson, AZ 85714


Tel: (520) 745-0001 Fax: (520) 745-0004

email: info-at-boeckeler.com


Sincerely


Chris Gleeson
President & Chief Executive Officer
Ventana Medical Systems, Inc.




From daemon Thu Oct 5 19:07:13 2000



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Thu, 5 Oct 2000 17:53:35 -0600
Subject: saw-tooth scan distortion: jeol35

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Brian,

what you see could be 60-Hz Noise. It follows the characteristics you are
describing (more 'teeth' with lower scan rate). Can you do a
back-of-the-envelope calculation of how many 'teeth' you get per second
(check exposure settings and line times). If it's around 60, you should try
to minimize the influence of 60 Hz Noise (ground loops, check all grounding
contacts, etc.)

Michael

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Brian McIntyre [mailto:mcintyre-at-optics.rochester.edu]
Sent: Thursday, October 05, 2000 8:04 AM
To: Microscopy-at-sparc5.microscopy.com


hi all-

i've installed a 35 and have some issues with a saw-tooth distortion in the
scan. it stays constant regardless of KV, but the number of saw-tooths
(teeth?) in a frame increases with slower scan rates. i suspected
vibration so i mounted the roughing pumps on a big foam block.....no
change. i also cycled off the chiller unit with no change, so its probably
not mechanical vibrations, unless there is another vibration causing
component i'm overlooking.

the distortion is also visible in the data field alphanumerics (they shift
and shimmy a little) with the beam turned off, so i don't suspect a HV
instability problem.

can i logically deduce that there is an electrical field leakage that is
messing up the scan? or a ground loop? what about cable placement? or a
problem with the 100V variac (its within 3' of the column).

right now the column is a bit dirty and needs cleaning....i've never seen,
for example, dirty apertures cause this type of distortion though.

any suggestions would be appreciated!

brian
----------------------------------------------
Brian McIntyre
Electron Microscopy Lab- River Campus
Univ of Rochester
Rochester, NY 14627
716-275-3058/4875



From daemon Thu Oct 5 19:35:24 2000



From: Pierre Hovington :      hovington.pierre-at-ireq.ca
Date: Thu, 5 Oct 2000 19:27:18 -0500
Subject: Annoncment for a FESEM workshop in Montreal

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




ANNOUNCEMENT
FE-SEM COURSE


It is with great pleasure that I am announcing our 2001 FE-SEM and Image
Analysis Courses

When: May 21-25, 2001

Guest Speaker: Dr. David C. Joy
Professor, Tennessee University
Marin Lagace,
Researcher, Hydro-Quebec Research Center

Where: IREQ (Hydro-Quebec Research Centre),
Varennes(Montreal), QC.,
Canada

Topics Covered: FE-SEM Components: gun, lenses, apertures, vacuum
technology...
Care & feeding of the FEG
The secrets of successful FE-Microscopy
Vacuum hygiene, cleaning and storing samples
Unwanted Beam Interactions
What is needed for High Resolution SEM
Image Content
Getting the most from your SEM
How to assess resolution
Low Voltage Work - High Resolution at low energy

LVSEM and charging
FESEM and Microanalysis
Low Energy EDS Work
plus so much more

This 3-day workshop will be devided in Lecture and
Practical Sessions

Image Analysis : Numerical filters description
Uses of mathematical morphology operators (erosion,
dilatation, etc)
Data analysis and interpretation
Recipes for common Image Analysis tasks


Costs: $1,500.00 (Can$) for FE-SEM and Image Analysis Workshops

$ 250.00 (Can$) for the Image Analysis Workshop ONLY


For more information (and/or for reservation) please contact:

Dr Pierre Hovington
tel: 450 652-8125
fax: 450 652-8905
e-mail: hovington.pierre-at-ireq.ca





From daemon Thu Oct 5 19:39:09 2000



From: Dusevich, Vladimir :      DusevichV-at-umkc.edu
Date: Thu, 5 Oct 2000 19:31:56 -0500
Subject: SEM Help needed on conductive adhesive

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


What about using Superglue? I routinely use it when
I need a good adhesion of samples. You really do not
need a conductive adhesive with a sample thickness of
about 3 mm - it's better just to draw a line with carbon
adhesive from the edge of a sample to a stud prior
conductive coating.

Vladimir Dusevich


-----Original Message-----
} From: "carla_aiwohi-at-usgs.gov"-at-sparc5.microscopy.com
To: Microscopy-at-sparc5.microscopy.com
Sent: 10/5/00 8:46 AM


Hello all,

I am attaching CP dried salmon skins (approx. 2-3 mm thick) to stubs
with
Electrodag 502 for secondary electron imaging. The adhesive is a
graphite
paint in MEK base (a substitute for TV Tube Koat). After ethanol
dehydration and CP drying in liquid CO2, the samples are relatively flat
or
slightly curved. However, once attached to the stub the skins develop a
noticeable curl which leaves a p between the stub surface and sample.
In
some cases I can see the "trail" in the adhesive left by the skin as it
was
curling! Filling the gap with more Electrodag is a waste of time
because
it evaporates and shrinks back away from the sample (thankfully it
doesn't
seem to produce more curling).

Could anyone suggest a better conductive adhesive? Also, is there any
difference between aqueous (eg. Aquadag colloidal graphite) and
MEK-based
media as to its effect on biological samples?

As an SEM novice any advice you could share with me is greatly
appreciated.
Thank you very much,

Carla Aiwohi
Western Fisheries Research Center
Seattle, WA
ph: (206) 526-6282 ext.242
fax: (206) 526-6654




From daemon Thu Oct 5 19:55:48 2000



From: andrewb-at-vsl.cua.edu
Date: Thu, 5 Oct 2000 20:48:28 -0400
Subject: Re: saw-tooth scan distortion: jeol35

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


You wrote:

} i've installed a 35 and have some issues with a saw-tooth distortion in
} the scan.

My 35C had an aperture heater which used to go into oscillation
and produce the kind of problem you describe. It has long since
been disconnected. It doesn't seem likely in your case because
you see the problem with the beam off, but I thought it was worth
mentioning just in case.


Sincerely yours,
Andy Buechele

Andrew C. Buechele, Ph.D.
Research Professor
Vitreous State Laboratory
The Catholic University of America
409 Hannan Hall
Washington, D.C. 20064
(202) 319-4995 FAX: (202) 319-4469



From daemon Thu Oct 5 20:55:35 2000



From: Nancy_Robertson :      Nancy_Robertson-at-dnr.state.ak.us
Date: Thu, 05 Oct 2000 17:51:19 -0800
Subject: Carbon Coated Grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hi,

I need good quality carbon coated grids for viewing purified plant virus
particles with TEM. I have had little success with "store bought" coated
grids. So unless someone could recommend a good cc grid source, I will
need to buy a carbon coater. Should I purchase a carbon coater using
flash evaporation or the more expensive carbon turbo evaporator?
In addition, could anyone recommend an economically good microtome for
thin sectioning plastic embedded specimens?

Thank You,

Nancy L. Robertson, Research Plant Pathologist
USDA/ARS
National Arctic Plant Germplasm Resources Unit
533 Fireweed Ave.
Palmer, Alaska 99645
phone: 907-746-9465
fax:907-746-2677
e-mail: nancy_robertson-at-dnr.state.ak.us



From daemon Fri Oct 6 01:57:22 2000



From: Chris Jeffree :      cjeffree-at-srv0.bio.ed.ac.uk
Date: Fri, 6 Oct 2000 09:10:04 +0100
Subject: Ge vs Si detectors - what are the shortcomings of Ge?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Another way to pick up noise and voltage is to wires wrapped around each
other. It is east to test just watch the screen and poke the wires with a
wood stick. If there is any problem in the wiring it will show up on the
screen. You only have to move them gently.

A wooden stick and light tapping can find a lot of problems. If you are
working in the high voltage section make sure it is a long dry stick. It
is great for finding intermittent problems. Just start tapping until the
problem appears or goes away and then narrow in on the problem.

When you check the frequency if it comes up 120 Hz florescent lights
become the number 1 suspect. You could be picking up electrical noise from
them or you have something photo
sensitive somewhere. Any silicone junction is sensitive to light. The
quick test is to turn off the lights. If they are the problem it should at
least get weaker.

Gordon
Gordon Couger gcouger-at-couger.com
Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00

----- Original Message -----
} From: "Mike Bode" {mb-at-Soft-Imaging.com}
To: "'Microscopy-at-MSA.Microscopy.Com'" {Microscopy-at-sparc5.microscopy.com}
Sent: Thursday, October 05, 2000 6:53 PM


Thank you all for your replies on this topic. Several people seem to
like Ge detectors for low keV work, and the replies have mostly been
very positive about their resolution and performance. Some authors
strongly advocate the use of Ge detectors for low kV, low probe
current work with light element samples (See, for example, Block-
van Hoek & Pinxter (1993) Chapter 1 in X-ray microanalysis in
biology edited by Sigee et al, Cambridge University Press). Their
opinion (and apparently yours) stands in contrast with the advice
given by all of the XRMA vendors I am speaking to, whose opinion is
that Si(Li) is the way to go (granted some of them don't sell Ge, so
they would obviously have to advocate Si). So my question is "what
is the Achilles heel of Ge detectors?". I am aware for example that
their potentially very favourable characteristics at low kV are spoiled
by the practical difficulty of manufacturing them without a large dead
layer. Is this a fatal flaw in practice for analysis of elements with z
{11? Are they less robust, less tolerant of thermal cycling? If
anyone can help me get to the bottom of this I would be most
grateful.

Chris

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Dr Chris Jeffree
University of Edinburgh
Biological Sciences EM Facility
Daniel Rutherford Building
King's Buildings EDINBURGH EH9 3JH
Tel: +44 (0) 131 650 5345
FAX: +44 (0) 131 650 6563

Inveresk Cottage, 26 Carberry Road,
Inveresk, Musselburgh, Midlothian EH21 8PR, UK
Tel: +44 (0) 131 665 6062 / Mobile 07710 585 401
FAX: +44 (0) 131 653 6248
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~



From daemon Fri Oct 6 03:44:22 2000



From: Alex :      tikhonov-at-mpi-halle.mpg.de
Date: Fri, 6 Oct 2000 10:36:19 MET
Subject: grinding plate

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All,

I am grinding a TEM specimen in a Multipol polishing machine, but a
grinding plate is covered with some stains of grayish color whose
origin I cannot identify. I tried to apply ethanol, atheton, and methanol
to them, but these proved in vain.

Please suggest any cleaning liquid to wipe the grinding plate clean.

Alex


Aleksandr Tikhonovsky, Ph.D.
tikhonov-at-mpi-halle.mpg.de
tel 49 345 5582 929
Max-Planck-Institut für Mikrostrukturphysik
Weinberg 2
D-06120 Halle
Germany



From daemon Fri Oct 6 03:59:49 2000



From: Allen R. Sampson :      ars-at-sem.com
Date: Fri, 6 Oct 2000 03:52:04 -0500
Subject: saw-tooth scan distortion: jeol35

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


You've had a lot of replies, but your symptoms are clear. In most cases,
this interference is caused by an electrical device mounted too close to
the SEM CRT - An EDS monitor, vacuum gauge controller, etc. If not, you
probably have a bad filter or bypass capacitor in a power supply that the
CRT uses. Since the effect is also seen in the alpha-numerics, it is
directly affecting the view CRT itself. Keeping the area around the CRT
clear of other instruments and checking for ripple in the power supplies to
the CRT should solve the problem. The effects of external instruments near
the view CRT are generally very local and movement of just a few inches can
make a huge difference.

It is not mechanical vibrations and the CRTs are not that suseptible to
electro-magnetic interference from distant sources.

If the alpha-numerics were not affected, I would suggest checking the
relative difference in the noise when using a low and high working
distance. If there is a noticably greater noise when at a larger working
distance, then electro-magnetic fields affecting the beam would be suspect.
The column of an SEM is actually well shielded from EM effects, as the
lenses are encased in large ferro-magnetic structures to provide the
focussing of the lens effects to the pole pieces. If lower working
distances are used (sample closer to the final pole piece) then the effects
of EM are reduced since they are primarily induced through the sample
chamber and affect the beam from the final pole piece to the sample
surface.

However, as I've said, if the alpha-numeric display is also affected, then
more than likely the CRT beam is being affected by something close by or by
a power supply to the CRT. The high voltage to the CRT (around 10KV) is
not a likely suspect as the record CRT is much more suseptable to HV
problems and in the JSM-35, as in most SEMs, is fed from the same supply.
You would have seen some obvious focus problems in your micrographs if the
high voltage was a problem. As the view CRT is an integral part of the
electronics console, ground loop problems are unlikely.

The record CRT and view CRT are located close together in this instrument
and as I recall they use the same power supplies. The record CRT is
probably recording a problem as well, but it may be greatly reduced. The
record cycle uses a greatly reduced scan speed, so these variations in the
view CRT are going to show up as reduced edge sharpness in your micrographs
or not at all if each record scan line is triggered by a zero crossing on
the 60Hz input (I don't recall if the JSM-35 does this).


Allen R. Sampson, Owner
Advanced Research Systems, St. Charles, Illinois 60174
voice 630.513.7093 fax 630.513.7092

-----Original Message-----
} From: Brian McIntyre [SMTP:mcintyre-at-optics.rochester.edu]
Sent: Thursday, October 05, 2000 9:04 AM
To: Microscopy-at-sparc5.microscopy.com


hi all-

i've installed a 35 and have some issues with a saw-tooth distortion in the
scan. it stays constant regardless of KV, but the number of saw-tooths
(teeth?) in a frame increases with slower scan rates. i suspected
vibration so i mounted the roughing pumps on a big foam block.....no
change. i also cycled off the chiller unit with no change, so its probably
not mechanical vibrations, unless there is another vibration causing
component i'm overlooking.

the distortion is also visible in the data field alphanumerics (they shift
and shimmy a little) with the beam turned off, so i don't suspect a HV
instability problem.

can i logically deduce that there is an electrical field leakage that is
messing up the scan? or a ground loop? what about cable placement? or a
problem with the 100V variac (its within 3' of the column).

right now the column is a bit dirty and needs cleaning....i've never seen,
for example, dirty apertures cause this type of distortion though.

any suggestions would be appreciated!

brian
----------------------------------------------
Brian McIntyre
Electron Microscopy Lab- River Campus
Univ of Rochester
Rochester, NY 14627
716-275-3058/4875





From daemon Fri Oct 6 05:03:39 2000



From: Allen R. Sampson :      ars-at-sem.com
Date: Fri, 6 Oct 2000 04:48:57 -0500
Subject: High Resolution noise

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Have an EDS system attached? The EDS dewars, being generally supported by
a cantilever structure, can be quite effective at coupling acoustic
vibrations into an SEM. The large area and thin metal construction of the
dewar as well as the large momentum arm of the cantilever provide for an
amplification of acoustics into the instrument. You might also try to
provide some form of isolated support and damping of the EDS system.

The size, shape and construction of the room can also have a considerable
effect. Certain configurations can amplify acoustic and construction
material mediated effects. Placement of the SEM within a particular room
can make a big difference. Particularly bad is the placement in an alcove
or bay of a room. Walls close to the sides of an instrument produce
complex acoustic patterns. Each wall surface represents a resonator that
can vibrate with unexpected sources. An instrument I had placed in this
situation in a clean room of a major alphabet soup named manufacturer had
incredible sensitivity to door closings and even footsteps in the adjoining
airlock and preparation areas.

Your mention of 5 jaggies begs the question - at what scan rate were these
viewed? What is the time span for each full scan? The SEM is an
incredibly sensitive seismometer. I've seen the effects of waves crashing
on the shores of an island as well as the vibrations from an M1-A1 Abrams
tank at 40 miles per hour on a nearby proving ground. It can often be
difficult to isolate the acoustic effects from the mechanically induced
effects (those coupled through solid materials). Most SEMs provide good
isolation from high frequency mechanical coupling, but low frequencies are
problematic.

Vibrational problems, unlike electro-magnetic problems, affect the beam
along it's entire path to some degree. In the column, most of these
effects are canceled by the fact that a movement that causes the beam to be
deflected outside of it's normal path will be countered by the increased
magnetic effect of the lenses as the beam goes off-axis. The greatest
effect though, is in the path from the final pole piece to the sample
surface. Your determination of the effect being the same from 4mm to 10mm
would probably not be true if you measured the difference from 4mm to 25mm.



Allen R. Sampson, Owner
Advanced Research Systems, St. Charles, Illinois 60174
voice 630.513.7093 fax 630.513.7092

-----Original Message-----
} From: Ben Craft [SMTP:bcraft-at-uci.edu]
Sent: Wednesday, October 04, 2000 9:52 PM
To: Microscopy-at-sparc5.microscopy.com


I'm trying to solve a high resolution noise problem with a XL-30
Phillips FESEM. The room that it is in was recently checked and was ok,
accept for some acoustic noise. To fix the noise I put up 3 inch sound
proofing foam and quieter ducting for the air condition. The noise
showed up in an image by a streak about 5 jaggies. After
putting up the foam the noise was reduced, but it is still there. The
noise is the same for 4mm working distance or a 10mm working distance.

Any one had a similar problem or have any advice?

Thanks,
Ben




#######
#####\_O -Ben Craft-
####/\/}
#### /"
### \








From daemon Fri Oct 6 07:00:38 2000



From: Ken Converse :      qualityimages-at-netrax.net
Date: Fri, 06 Oct 2000 07:39:49 -0700
Subject: Re: saw-tooth scan distortion: jeol35

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Brian McIntyre wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} hi all-
}
} i've installed a 35 and have some issues with a saw-tooth distortion in the
} scan. it stays constant regardless of KV, but the number of saw-tooths
} (teeth?) in a frame increases with slower scan rates. i suspected
} vibration so i mounted the roughing pumps on a big foam block.....no
} change. i also cycled off the chiller unit with no change, so its probably
} not mechanical vibrations, unless there is another vibration causing
} component i'm overlooking.
}
} the distortion is also visible in the data field alphanumerics (they shift
} and shimmy a little) with the beam turned off, so i don't suspect a HV
} instability problem.
}
} can i logically deduce that there is an electrical field leakage that is
} messing up the scan? or a ground loop? what about cable placement? or a
} problem with the 100V variac (its within 3' of the column).
}
} right now the column is a bit dirty and needs cleaning....i've never seen,
} for example, dirty apertures cause this type of distortion though.
}
} any suggestions would be appreciated!
}
} brian
} ----------------------------------------------
} Brian McIntyre
} Electron Microscopy Lab- River Campus
} Univ of Rochester
} Rochester, NY 14627
} 716-275-3058/4875
Brian,
You didn't say whether or not the sawtooth shows up on micrographs. If
the problem were 60 Hz, they would not be apparent because the record
scan speeds are sync'd to the line frequency. If you DO see them on
micrographs, start by looking at your low voltage supplies, the CRT HV
supply and the scan generator signals.

The problem has nothing to do with your column, since the characters
show the problem. CRT HV instabilities should show up more the further
from the center of the CRT you get. You should also be getting both
light and dark lines as the vertical, as well as the horizontal, will be
affected.

If you don't see the above symptoms, look at both scan signals going to
the CRT. You may see noise on the horizontal signal, but if the
sawtooth is showing up in the image, also, you probably won't see the
noise on the horizontal signal to the column, because they aren't
syncronized. If they both had the same noise, the image would look fine
even though the alphanumerics shimmied.

Because of the alphanumerics, there is some processing of the CRT scan
signals after the scan generator that does not happen to the scan signal
sent to the mag control/column. A good place to look.

If the problem only occurs on 1 CRT, look at its drivers.

Ken Converse
owner
Quality Images
third party SEM service
Delta, PA



From daemon Fri Oct 6 07:40:52 2000



From: LungoFam-at-gte.net
Date: Fri, 6 Oct 2000 07:37:13 -0500
Subject: Ask-A-Microscopist: IgG question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Email: LungoFam-at-gte.net
Name: William Lungo
School: East Los Angeles College
State: California

Question: Protocol for processing biological specimens for IgG immuno gold
using LR White embedding media, for electron Microscopy

---------------------------------------------------------------------------





From daemon Fri Oct 6 08:17:09 2000



From: Ladd Research :      ladres-at-worldnet.att.net
Date: Fri, 06 Oct 2000 09:12:05 -0400
Subject: Re: Carbon Coated Grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Nancy,

If you have not tried our carbon coated grids, we believe we make pretty
good ones. If you have tried ours, please let me know what you didn't
like and we can correct it since we make them up fresh for each order,
and can make them thinner, thicker or whatever you wish.
In a worst case scenario, we make what we consider the best evaporator
in the business.

John Arnott

--

LADD RESEARCH
131 Dorset Lane
Williston, VT 05495 USA

web site http://www.laddresearch.com

TEL 1-800-451-3406 (US) or 1-802-878-6711 (anywhere)
FAX 1-802-878-8074
e-mail sales-at-laddresearch.com

Microscope Supplies Since 1955

Nancy_Robertson wrote:
}

}
} Hi,
}
} I need good quality carbon coated grids for viewing purified plant virus
} particles with TEM. I have had little success with "store bought" coated
} grids. So unless someone could recommend a good cc grid source, I will
} need to buy a carbon coater. Should I purchase a carbon coater using
} flash evaporation or the more expensive carbon turbo evaporator?
} In addition, could anyone recommend an economically good microtome for
} thin sectioning plastic embedded specimens?
}
} Thank You,
}
} Nancy L. Robertson, Research Plant Pathologist
} USDA/ARS
} National Arctic Plant Germplasm Resources Unit
} 533 Fireweed Ave.
} Palmer, Alaska 99645
} phone: 907-746-9465
} fax:907-746-2677
} e-mail: nancy_robertson-at-dnr.state.ak.us



From daemon Fri Oct 6 08:53:42 2000



From: jshields-at-cb.uga.edu
Date: Fri, 6 Oct 2000 09:53:06 -0400
Subject: refraction index question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


------- Forwarded message follows -------
} From: Charles Keith {chkeith-at-cb.uga.edu}
To: farmer-at-emlab.cb.uga.edu
Date sent: Thu, 5 Oct 2000 09:47:00 -0400 (Eastern Daylight
Time) Priority: NORMAL

Mark -

Could you post a request to your microscopy listserv for
the index of refraction of Dulbecco-Vogt PBS? I have done
so to sci.techniques.microscopy

----------------------
Charles Keith
Associate Professor, Department of Cellular Biology
(http://www.uga.edu/~cellbio)
University of Georgia
chkeith-at-cb.uga.edu

John Shields
EM Lab
151 Barrow Hall
UGA campus
2-4080
fax: 2-4271
jshields-at-cb.uga.edu



From daemon Fri Oct 6 08:54:47 2000



From: Julie Piraino :      piraino-at-sms.si.edu
Date: Fri, 06 Oct 2000 09:52:34 -0400
Subject: re:CoolPix 990 to Axioscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Martin Microscope Company of Easley, SC, USA makes an adapter for the Coolpix 990 to fit the eyepiece tube of any microscope (both 23mm and 30mm diameter tubes) for $300. They can also get you an adapter for C-mount. Their phone number is 864-242-3424 or 864-859-2688

} } } Gary Radice {gradice-at-richmond.edu} 10/05/00 01:47PM } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


You might also try contacting Bunton Instruments in the US:

{http://www.buntgrp.com/}

They sell microscope adaptors for the CoolPix. The adaptors are not listed
on their web site, yet, but call or e-mail them for information. I know
they are taking orders for adaptors now and I was told that they would be
shipping "around the end of October."

I have no connection with Bunton Instruments other than as a customer.

Gary P. Radice gradice-at-richmond.edu
Associate Professor of Biology 804 289 8107 (voice)
University of Richmond 804 289 8233 (FAX)
Richmond VA 23173 http://www.science.richmond.edu/~radice







From daemon Fri Oct 6 10:18:53 2000



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Fri, 06 Oct 2000 09:16:29 -0500
Subject: Re: Ge vs Si detectors - what are the shortcomings of Ge?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I thought the only drawback was cost. How much of a cost differential are
you being quoted?

I thought all manufacturers had to pick through crystals to find ones
suitable for EDS detectors in regard to dead layer, impurity, etc. If good
Ge chips are harder to fine, it would mean more expensive detectors.

We haven't tried thermally cycling our detector. I have heard that voltage
applied to Si(Li) detectors while warm will lead to a loss of Li, ruining
the detector. I don't know what effect it would have on a Ge detector.
However, my manager is reluctant to find out if there would be a problem so
we just keep our system cool.

Warren

At 09:10 AM 10/6/2000 +0100, you wrote:

} Thank you all for your replies on this topic. Several people seem to
} like Ge detectors for low keV work, and the replies have mostly been
} very positive about their resolution and performance. Some authors
} strongly advocate the use of Ge detectors for low kV, low probe
} current work with light element samples (See, for example, Block-
} van Hoek & Pinxter (1993) Chapter 1 in X-ray microanalysis in
} biology edited by Sigee et al, Cambridge University Press). Their
} opinion (and apparently yours) stands in contrast with the advice
} given by all of the XRMA vendors I am speaking to, whose opinion is
} that Si(Li) is the way to go (granted some of them don't sell Ge, so
} they would obviously have to advocate Si). So my question is "what
} is the Achilles heel of Ge detectors?". I am aware for example that
} their potentially very favourable characteristics at low kV are spoiled
} by the practical difficulty of manufacturing them without a large dead
} layer. Is this a fatal flaw in practice for analysis of elements with z
} {11? Are they less robust, less tolerant of thermal cycling? If
} anyone can help me get to the bottom of this I would be most
} grateful.
}
} Chris

----------------------
Warren E. Straszheim
Materials Analysis and Research Lab
Iowa State University
23 Town Engineering
Ames IA, 50011-3232

Ph: 515-294-8187
FAX: 515-294-4563

E-Mail: wesaia-at-iastate.edu
Web: www.marl.iastate.edu

Scanning electron microscopy, x-ray analysis, and image analysis of materials
Computer applications and networking



From daemon Fri Oct 6 10:34:42 2000



From: Christine Brantner :      brantner-at-codon.nih.gov
Date: Fri, 6 Oct 2000 11:42:23 -0400
Subject: CoolPix 950 to Axioscope 2 and cable release

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Good day

We have a new Nikon adaptor from Image Systems that allows us to
attach our CoolPix 950 to our Zeiss Axioscope 2 via the C-mount or
through the eye tube if we wanted to do it that way. Image Systems
also sells something they call a cable release adaptor which is a
"fancy piece" that hooks onto the camera over the shutter button so
that a cable release can be screwed in there. This way we can take a
picture without putting pressure on the adaptor set-up or programming
the camera to do it. Image Systems in Columbia Maryland can be
reached at 410-995-0748.

Just a recent customer.

Chris
--
:):):):):):):):):):):):):):):):):):):):):):):):):):):):):):)

Christine A. Brantner, Ph.D.

Biologist/Lab Manager

National Institutes of Health
National Institute of Neurological Diseases and Stroke
Lab of Neurobiology
Section of Analytical Cell Biology
Bldg 36, room 2A21 phone 301-435-2803
36 Convent Dr fax 301-480-1485
Bethesda, MD 20892-4062



From daemon Fri Oct 6 10:43:51 2000



From: David Joswiak :      joswiak-at-orca.astro.washington.edu
Date: Fri, 6 Oct 2000 08:41:43 -0700 (PDT)
Subject: Re: Carbon Coated Grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Nancy - Try Ladd Research (www.laddresearch.com) for high quality grids.
I've been using their C-coated grids for several years now and have found
that nearly every grid has been perfect. Their grids are somewhat more
expensive than those from other manufacturers, but well worth the price if
you need good quality.

I haven't tried purchasing grids from every manufacturer so there may be
other companies that also produce fine C-coated grids.

Dave Joswiak
Dept. of Astronomy
Univ. of Washington
Seattle, WA 98195
(206)543-7702
joswiak-at-astro.washington.edu


On Thu, 5 Oct 2000, Nancy_Robertson wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} Hi,
}
} I need good quality carbon coated grids for viewing purified plant virus
} particles with TEM. I have had little success with "store bought" coated
} grids. So unless someone could recommend a good cc grid source, I will
} need to buy a carbon coater. Should I purchase a carbon coater using
} flash evaporation or the more expensive carbon turbo evaporator?
} In addition, could anyone recommend an economically good microtome for
} thin sectioning plastic embedded specimens?
}
} Thank You,
}
} Nancy L. Robertson, Research Plant Pathologist
} USDA/ARS
} National Arctic Plant Germplasm Resources Unit
} 533 Fireweed Ave.
} Palmer, Alaska 99645
} phone: 907-746-9465
} fax:907-746-2677
} e-mail: nancy_robertson-at-dnr.state.ak.us
}
}




From daemon Fri Oct 6 10:50:35 2000



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Fri, 06 Oct 2000 08:47:15 -0700
Subject: Re: SEM Help needed on conductive adhesive

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Carla,
The double-sided, conductive tabs that are sold in most EM supply catalogues
are excellent and provide a good, strong hold. I use DAG 154 from Acheson
Colloids, which is colloidal carbon in iso-propanol and ethanol, as a
conductive paint to connect the coated surface to the stub.
At 06:46 AM 10/5/00 -0700, you wrote:
}
} Hello all,
}
} I am attaching CP dried salmon skins (approx. 2-3 mm thick) to stubs with
} Electrodag 502 for secondary electron imaging. The adhesive is a graphite
} paint in MEK base (a substitute for TV Tube Koat). After ethanol
} dehydration and CP drying in liquid CO2, the samples are relatively flat or
} slightly curved. However, once attached to the stub the skins develop a
} noticeable curl which leaves a gap between the stub surface and sample. In
} some cases I can see the "trail" in the adhesive left by the skin as it was
} curling! Filling the gap with more Electrodag is a waste of time because
} it evaporates and shrinks back away from the sample (thankfully it doesn't
} seem to produce more curling).
}
} Could anyone suggest a better conductive adhesive? Also, is there any
} difference between aqueous (eg. Aquadag colloidal graphite) and MEK-based
} media as to its effect on biological samples?
}
} As an SEM novice any advice you could share with me is greatly appreciated.
} Thank you very much,
}
} Carla Aiwohi
} Western Fisheries Research Center
} Seattle, WA
} ph: (206) 526-6282 ext.242
} fax: (206) 526-6654

Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca




From daemon Fri Oct 6 10:52:25 2000



From: michael shaffer :      epmalab-at-darkwing.uoregon.edu
Date: Fri, 6 Oct 2000 08:39:04 -0700
Subject: RE: Digital microscope camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Robin writes ...

} } I'm looking to replace my two year old Polaroid Digital
} Microscope camera ...

If your camera-microscope interface is via 1x c-mount, then the Nikon
CP990 and the 1x c-mount adapter is a very good choice ... or convert
your microscope tube to c-mount (1x).

A point should be made ... the CP cameras acquire images to an
internal memory card ("Compact Flash") ... than cannot acquire direct
to a computer. The transfer is instead accomplished at some other
time ... either a direct connection, camera to a serial or USB port,
or (better/faster) by removing the memory card and using a "Compact
Flash" card reader. This might be a hassle for a workstation type
microscope, but if freedom of location is important, then probably
works better.

cheerios, =shAf= :o)

{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {}
Michael Shaffer, R.A. - mshaf-at-darkwing.uoregon.edu
Geological Science's Electron Probe Facility - University of Oregon
http://epmalab.uoregon.edu/






From daemon Fri Oct 6 11:54:30 2000



From: William F. Tivol :      wft03-at-health.state.ny.us
Date: Fri, 6 Oct 2000 12:50:58 -0400
Subject: Re: Ge vs Si detectors - what are the shortcomings of Ge?

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Dear Warren,

I thought the only drawback was cost.

Another drawback is the need to keep them at 77 K all the time (see below).

I have heard that voltage applied to Si(Li) detectors while warm will lead to a
loss of Li, ruining the detector.

Not actual loss of Li. To produce a Si(Li) detector, Li atoms are diffused
into a Si crystal, which is then subjected to a bias voltage and heated. Li
moves toward the cathode in such a way as to compensate for excess electron
acceptors, and this results in a large fraction of the detector volume being
depleted of carriers and, thus, having a very low dark current. When warmed to
room temperature in the presence of bias voltage, Li is redistributed, and the
detector no longer has the necessary large depletion region. It is possible to
get the same effect with ultra pure Si or Ge, but such detectors--called
"intrinsic"--are very expensive due to the stringent limit for impurities. It
is now known that in the absence of bias voltage Si(Li) detectors can be at room
temperature for a while without damage (although 15 years ago that was not
thought to be the case), but Ge(Li) detectors must be kept at 77 K even when no
voltage is applied.
Yours,

Bill Tivol
Wadsworth Center
Albany NY
(518) 473-7399 WFT02-at-health.state.ny.us





From daemon Fri Oct 6 13:53:55 2000



From: Alan Fox :      fox-at-nps.navy.mil
Date: Fri, 06 Oct 2000 11:47:59 -0600
Subject: Ge vs SiLi

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Chris
I also have an Oxford intrinsic Ge detector on my SEM and enjoy
all the advantages described by previous correspondents. However, as the
max voltage on my SEM is 30kV the heaviest element for which I can get K
line info (which is preferable to L-lines for quantification) with
decent S/N ratio is around Sn (Z = 50). This of course depends on the
amount of tin present in your sample.

If you have a Ge detector fitted to a TEM operating at 200 kV, then, in
principle, you should be able to detect K lines for all elements. As a
result, installation of an intrinsic Ge detector on a TEM may also be a
good thing.

Alan Fox

Alan Fox
Center for Materials Science and Engineering
Naval Postgraduate School
Monterey
CA 93943

Tel (831) 656 2142
Fax (831) 656 2238




From daemon Fri Oct 6 14:04:59 2000



From: Dr. Raj Lartius :      rlartius-at-novascan.com
Date: Fri, 06 Oct 2000 14:03:41 -0500
Subject: Where to list a late model SEM?

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Hi Everyone,

My company is interested in selling a fairly new SEM and we are having a
difficult time finding an appropriate place to list it. Can anyone give
some suggestions. Also is there any kind of depreciation formula for
estimating an appropriate valuation.

Thanks

Raj

**********************************************
Dr. Raj Lartius, CEO
NovaScan Technologies
Iowa State University Research Park
2501 North Loop Drive
Ames, Iowa USA 50010

Email: rlartius-at-novascan.com
Voice: 515-795-3164
Fax: 515-795-4414
**********************************************
"Innovative Tools to Explore the Microworld"



From daemon Fri Oct 6 14:20:09 2000



From: Norm Olson :      nho-at-bilbo.bio.purdue.edu
Date: Fri, 6 Oct 00 14:16:38 -0500
Subject: Freeze-plunge device availability

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The Microscopy ListServer -- Sponsor: The Microscopy Society of America


EM-listers: I frequently get asked for commercial sources for devices
that plunge freeze TEM grids into a cryogen such as liquid ethane. We
have our own in-house developed device so I have never kept up with what
is on the market. Available devices also seem to come and go so some of
my old sources no longer are current. Could I get vendors that have
these devices to respond to me off the list. I would appreciate a
reference to a web page if one exists and whatever ordering information
is available. I will be posting this information on our lab web page and
if there is enough interest on this list I can also post the responses.

Thanks
Norm Olson

*******************************************
Norm Olson
Senior Research Electron Microscopist
Department of Biological Sciences
Lilly Hall of Life Sciences
Purdue University
West Lafayette, IN 47907

Phone: 765-494-5643
FAX: 765-496-1189
email: nho-at-bragg.bio.purdue.edu
http://bilbo.bio.purdue.edu/~nho/index.htm
http://bilbo.bio.purdue.edu/~baker/

*******************************************




From daemon Fri Oct 6 14:37:28 2000



From: Walck, Scott D. :      walck-at-ppg.com
Date: Fri, 6 Oct 2000 15:35:15 -0400
Subject: alternate fluid for ultramicrotomy

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What other liquids can be used for floating off thin sections other than
water? I have a sample that swells in water. It swells slowly in alcohol.
Thanks in advance.

-Scott Walck




From daemon Fri Oct 6 14:44:28 2000



From: Plybon, Charles :      Charles.Plybon-at-fwc.state.fl.us
Date: Fri, 6 Oct 2000 15:37:39 -0400
Subject: Lugol's Iodine as a Fixative

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I have been asked about the mechanism of Lugol's Iodine as a fixative and I
am not sure how it works. Is it a coagulent or anitcoagulent fixative?
Does it cross-bind proteins like formaldehyde or formalin? It is used often
for fixing dinoflagellate samples and it seems to stabilize the structure of
the organisms fairly well without alcohol. Can anyone explain to me the
mechanism?

Charles T. Plybon
Florida Marine Research Institute
Research Staff
Aquatic Health Group
Tel: (727) 896-8626
e-mail: charles.plybon-at-fwc.state.fl.us




From daemon Fri Oct 6 15:26:31 2000



From: Timothy Schneider :      Timothy.Schneider-at-Mail.TJU.EDU
Date: Fri, 6 Oct 2000 16:15:54 -0400
Subject: L.R. WHITE

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List severs:

Does any one out there etch L. R. White sections? With
sodium-meta-periodate, or something else? Does it really make a difference?
Tim

Timothy G. Schneider
Director of Electron Microscopy
Department of Pathology
Room 229 Jefferson Hall
Thomas Jefferson University
1020 Locust St.
Philadelphia Pa. 19107
215-503-4798 work
610-613-8170 cellular
timothy.schneider-at-mail.tju.edu




From daemon Fri Oct 6 15:31:06 2000



From: Caroline Schooley :      schooley-at-mcn.org
Date: Fri, 6 Oct 2000 13:22:56 -0700
Subject: Video microscopy needed

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Please reply directly to Somayyah:

Fabian-Baber Communications is a multimedia educational production
company. We are currently producing a ten-part video series on the human
body to be used in middle-school classrooms. We are looking for motion
microscopy of human cells, such as blood cells coursing through arteries,
cells during mitosis, etc. If you are interested in contributing to this
project, or know of a good resource for these images, please contact ,
Somayyah Siddiqi, at sam-at-fabian-baber.com or 610 623 7812. Thanks so much!

Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html





From daemon Fri Oct 6 16:27:32 2000



From: hadden-at-wingate.edu (Lee Hadden)
Date: Fri, 06 Oct 2000 17:26:13 -0400
Subject: CPD user feedback

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To CPD users:

I would appreciate the benefit of your experience and knowledge in
answering
several questions for me as we prepare to purchase our CPD for use in
teaching
undergraduate biology courses and student/faculty research.

What is your recommendation re the following:

having / not having a viewing port: [I've heard a few recommendations
for having a
viewing port in a CPD [all accompanied by warnings re the inherent
danger]. The
only CPD I've used [years ago] had a port which I liked, especially for
teaching
purposes. I also been asked "Why would you want one?"

chamber size and orientation: vertical or horizontal:

type[s] of specimen holders:

electrically heated vs. hot water heated:

manufacturers and models that you use or have used recently:

costs of fluids and materials used in / with your CPD for a typical run:

compared to other models you might be familiar with:
Specifically, is it too much more expensive to run a Polaron jumbo
chamber vs.
their regular size chamber? [again, for biological material].

What would you look for in your next CPD?

Thanks for your input and time -- they're greatly appreciated.

Lee Hadden
Department of Biology
Wingate University
Wingate, NC 28174
hadden-at-wingate.edu
704.233.8238.






From daemon Fri Oct 6 16:30:09 2000



From: Tamara Howard :      howard-at-cshl.org
Date: Fri, 6 Oct 2000 17:24:05 -0400 (EDT)
Subject: Re: L.R. WHITE

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If you are trying to "antigen retrieve" on LR White, there is a nifty
paper - Stirling and Graff, 1995, Journal of Histochem. and Cytochem.,
43:115-123. "Antigen Unmasking for Immunoelectron Microscopy: Labeling is
Improved by Treating with Sodium Ethoxide or Sodium Metaperiodate, then
Heating on Retrieval Medium"

Tamara Howard
CSHL

On Fri, 6 Oct 2000, Timothy Schneider wrote:

} ------------------------------------------------------------------------
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} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
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} -----------------------------------------------------------------------.
}
}
} List severs:
}
} Does any one out there etch L. R. White sections? With
} sodium-meta-periodate, or something else? Does it really make a difference?
} Tim
}
} Timothy G. Schneider
} Director of Electron Microscopy
} Department of Pathology
} Room 229 Jefferson Hall
} Thomas Jefferson University
} 1020 Locust St.
} Philadelphia Pa. 19107
} 215-503-4798 work
} 610-613-8170 cellular
} timothy.schneider-at-mail.tju.edu
}
}
}




From daemon Fri Oct 6 20:12:14 2000



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Fri, 06 Oct 2000 21:12:28 -0500
Subject: carbon coated grids

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-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Nancy Robertson wrote:
===============================================================
} I need good quality carbon coated grids for viewing purified plant virus
} particles with TEM. I have had little success with "store bought" coated
grids.
} So unless someone could recommend a good cc grid source, I will need to
buy a
} carbon coater. Should I purchase a carbon coater using flash evaporation
or the
} more expensive carbon turbo evaporator? In addition, could anyone
recommend an
} economically good microtome for thin sectioning plastic embedded specimens
?
================================================================
SPI Supplies has produced, in house, with our own (in-house) experienced
staff, carbon coated grids, including holey and lacey types, for many years.
I hope your less-than-expected results from purchased grids were not our
grids, because we really are set up to make them however one does want to
make them, mainly because we have our own TEM facilities at our side to
inspect what we have made. In any case, many laboratories worldwide depend
on the SPI carbon coated grids and we are not aware of any problems that
anyone has encountered with them (lots not passing inspection are rejected
and not sent to customers). Some want them thicker, some want them thinner,
some want them with large holes, some smaller holes, but we can make them
however someone wants them to be!

With regard to your question: "Should I purchase a carbon coater using
flash evaporation", I will assume that you are asking about the "carbon
coaters" that are operated with a rotary vane mechanical pump, usually in
tandem with a sputter coater. We are not aware of anyone that has ever
gotten acceptable carbon coatings that could be used as support films by TEM
using such an equipment approach. Only a diffusion pumped vacuum system, or
better, seems to be capable of producing the kind of carbon coatings that
would meet that standard. A number of firms offer vacuum evaporators, and
we have found the vacuum evaporators made by VG Microtech and also, Denton
Vacuum to be especially good for this kind of applications. Quite possibly,
others are equally good, but our experience has been with these two
particular manufacturers. Note: We do not have any interest, financial or
otherwise, in either Denton Vacuum or VG Microtech.

We had the good fortune of learning about a paper soon to be published
comparing carbon grids from different commercial sources. The author is P.
J. F. Harris, Dept. of Chemistry, U. Reading, Whiteknights, Reading RG6 6AD
UK. The title is: Carbonaceous Contaminants on Carbon Support Films for
Transmission Electron Microscopy, Carbon 1 (2000).

If Dr. Harris is correct, then one possible solution, since they are made
using a different approach (but were not studied by Dr. Harris), might be
the Quantifoil grids (my opinion), see URL
http://www.2spi.com/catalog/grids/quantifoil-carbon-films.html

They are produced using a different kind of process and therefore would be
expected to not have present some of the ambiguous features found on the
carbon films studied in the paper mentioned above.

I don't know if this paper has been published yet or not, but I could send
to any one requesting it, the entire preprint of the paper via pdf file.
Such requests should be made off-line. Give me a day or two to respond.

Disclaimer: SPI Supplies has manufactured since 1975 custom made carbon
support films for TEM applications. Grids made by SPI were one of those
studied in the above referenced article. However, it is entirely possible
that given some of the new demands being made on carbon coated grids
generally, especially for high temperature stability, that one should
consider as an alternative, the SPI Silicon Nitride Membrane Grids, as
described on URL
http://www.2spi.com/catalog/instruments/silicon-nitride.html

Chuck

===========================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.spi.cc
########################
============================================





From daemon Fri Oct 6 20:12:14 2000



From: ard-at-ansto.gov.au (Arthur Day)
Date: Sat, 7 Oct 2000 11:06:01 +1000
Subject: RE: High Resolution noise

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Allen R. Sampson wrote:
}
} Have an EDS system attached? The EDS dewars, being generally supported by
} a cantilever structure, can be quite effective at coupling acoustic
} vibrations into an SEM. The large area and thin metal construction of the
}

EDS dewars:
In regard to EDS dewars and acoustic vibrations, if there is enough ice or
dirt in the dewar to nucleate bubbles in the LN2 then you might get
sufficient vibration as the LN2 boils off. You can often hear this simply
by putting your ear against the dewar, especially while the LN2 is at a
relatively low level in the dewar. Perhaps in really vibration sensitive
systems such as a FESEM this could be another potential problem? The sound
of the boiling can range from a continuos fizz to an occasional intense
"bump" similar to the effect that you can get just as water begins to boil
in a pot on a stove.

Stage motors:
We had a bad problem with stage motors once. If you have a motorised stage
then any AC ripple from bad power supply filtering remaining on top of the
DC holding current can transmit enough vibration via the motor coils to
cause problems at high magnification. Sometimes you can "feel" these kinds
of vibrations in the motor bodies. Since this holding current is present
even when the motors are not actually moving the stage, you have to
actually shut off the power to the stage motors to determine if this is the
cause.


Arthur Day, Electron Microscope Unit Phone: 61-2-9717-3457
Ansto Materials Division Fax: 61-2-9543-7179
PMB 1, Menai (Sydney), NSW, 2234 Email: ard-at-ansto.gov.au
Australia www: http://www.ansto.gov.au/





From daemon Sat Oct 7 07:26:55 2000



From: MESnesm-at-aol.com
Date: Sat, 7 Oct 2000 08:16:55 EDT
Subject: NESM October Meeting

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To all:

The October meeting of NESM (New England Society for Microscopy) will be held
on October 17th at FEI Company in Peabody, MA.

Pre-registration deadline is Friday, October 13th. Please contact Mary
McCann, NESM Treasurer at (617)484-7865 or by e-mail: mccanns-at-tiac.net.
Registration is $5.00 for members and $20.00 for non-members (this fee
includes a current-year membership in NESM).

The meeting will begin with a tour of FEI Company at 5:30pm. Also at this
time, Dr. Ronald Mueller of Bristol Myers Squibb will speak on the utility
of SEM within
the Pharmaceutical Industry. A buffet dinner will follow at 6:00pm.

The scientific presentations will begin at 7:00pm. The speakers are:
Dr. Ben Fabry of Harvard School of Public Health, Boston, MA-"Magnetic
Twisting
Cytometry with Sub-Micron Optical Detection"

Dr. Dan Friend of Brigham and Women's Hospital, Boston, MA-"Thin Section:
Freeze Fracture Correlates-A Visit to the Good Old Days"

Barbara Foster-President, Microscopy, Marketing and Education, Springfield,
MA-
"Bridging the Microscopy-Spectroscopy Chasm"

Peggy Sherwood
Corresponding Secretary, NESM




From daemon Sat Oct 7 11:28:15 2000



From: Gary Gaugler :      gary-at-gaugler.com
Date: Sat, 07 Oct 2000 09:19:35 -0700
Subject: Options for Coolpix 990 to Zeiss (long)

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Thanks to all who sent suggestions and options for
connecting the CP990 to Zeiss Axioskop. There are
several options. After examining each one, here is
a good approach to using the CP990 on an Axioskop.

The first accessory is the attachment of the camera
to the 'scope. This is done using an Axioskop trinoc
photo port adapter to C-mount. Then, a CP990 thread
ring to C-mount adapter is installed. These two
adapters then mate the CP990 to the photo port of
the Axioskop. Optmem makes all of the pieces needed
to make the mating between 'scope and camera.
The mate from photo port to C-mount can be done two
ways.

Diagnostic Instruments makes a 38mm ISO mount
(PN DZA, $275) which also accepts items for Leitz.
This same ISO mount onto the Zeiss will accept
a PA-41A adapter for Nikon F mount cameras.
Optem makes a coupler DC10NN ($80) which
mates the 38mm ISO port to C-mount. Then,
their 25-70-14-03 ($320) is the CP990 to C-mount
adapter. This completes the adaption for a total
cost of $275 + $80 + $320 = $675.

Optem's alternate method is the DC-10AM ($150)
which directly mounts in the trinoc photo port and
provides a C-mount. Then, this mates with the
same 25-70-14-03 CP990/C-mount adapter.
Total cost of this approach is $150 + $320 = $470.

Zeiss also makes an Axioskop photo port C-mount
adapter. This part number is 45 29 95. They
can be found used from between $125-$250.
Then, the Optem 25-70-14-13 CP990 adapter is
used to complete the mate.

When using the CP990, or any other camera for that
matter, vibration is a key issue. In this respect the
CP990 has an advantage since it does not have a
mechanical shutter as do other CCD cameras.
But one still needs to reduce vibration when actually
taking a pix. This is done via a remote cable release.
There are several aftermarket units which perform
this function. However, they are rather awkward and
large. The best approach is the Nikon EU-1 wired
remote ($195 list price). This is a small hand held
puck which plugs into the USB port on the CP990.
Pressing the large button down half way is like
doing so on the CP990 shutter release button. Pressing
all the way down takes the pix. The puck also indicates
on an LCD how many frames are left.

When doing 'scope work, I recommend the Nikon EH-31
AC adaptor ($50 list price). This avoids drain on the
camera batteries.

For storage, the CP990 uses CF type 1 media. The IBM
microdrive will not work in this camera. Since the
camera stores images in JPEG format, the file size of
each image will vary according to how the image
compresses. So far, the largest file I have taken is
just over 1MB. I am using 128MB CF media. So I can
reasonably expect to get about 100 frames per CF.
I have two of these CF media. When one is full, it
is pulled and replaced with an empty one. The full one
is emptied using a Microtech USB CF reader ($38).

Finally, image focusing has always been a problem for me.
The CP990 changes all of that. Using the CP990 real time
NTSC video output jack, one can watch the image on a
separate TV monitor (b/w or color) and adjust for perfect
focus. Then take the pix. If you want the camera's LCD
unit to stay on indefinitely while shooting, use the setup
menu and disable the power off feature.

That's about it for the CP990. Next is the Fuji Finepix S1 Pro.
One camp says it will work on a microscope. Another
camp says it won't. The camera only does automatic
modes when using a lens. When the lens is removed,
the camera becomes dumb. What is will actually do
remains to be seen. The folks at ElectroImage say
that it does work.

Contacts: Ron; Optmem NY. 716.223.2370
Mike Reyes, Diagnostic Instruments. Extension 32
Zeiss....good luck.

cheers,
gary g.






From daemon Sat Oct 7 14:35:57 2000



From: Plybon, Charles :      Charles.Plybon-at-fwc.state.fl.us
Date: Sat, 7 Oct 2000 15:26:49 -0400
Subject: Lugol's Iodine as a Fixative

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For some time, Lugol's Iodine has been used not only to stain certain cells
but also to fix and preserve samples of dinoflagellates and other
zooplankton. I am curious as to what the mechanism is for stabilizing and
fixing with Lugol's. Coagulent or anitcoagulent, does it cross-bind
proteins preserving cellular integrity? Can anyone help?

Charles T. Plybon
Florida Marine Research Institute
Research Staff
Aquatic Health Group
Tel: (727) 896-8626
e-mail: charles.plybon-at-fwc.state.fl.us




From daemon Mon Oct 9 01:00:33 2000



From: klaus neumann :      bikneu-at-krzsun.med-rz.uni-saarland.de
Date: Mon, 09 Oct 2000 08:00:03 +0200
Subject: alternate fluid for ultramicrotomy

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To Scott Walck:
Why not try a DRY (diamond) knife?
Klaus Neumann
Med. Biologie
Universität des Saarlandes
D-66421 Homburg
Germany




Dr. Klaus Neumann
3.5 Med. Biologie
Universität
D-66421 Homburg
Tel: ++49-6841-16-62 55
Fax: ++49-6841-16-62 56
http://www.med-rz.uni-sb.de/med_fak/biologie




From daemon Mon Oct 9 08:31:48 2000



From: Marek Malecki :      mmm-at-biomail.ucsd.edu
Date: Mon, 9 Oct 2000 08:27:56 -0500
Subject: Re: alternate fluid for ultramicrotomy

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Scott,
Glycerol at different concentration (87% as a starter). I use it for PVP
and PVA blocks.
Marek.


At 15:35 2000-10-06 -0400, you wrote:
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Director and Principal Investigator

Molecular Imaging Laboratories
University of California, San Diego

address: 1500 Suite Bonner Hall, 9500 Gilman Drive, La Jolla, CA92093-0368
phone - office: 8588223373
phone - lab: 8588223715
fax: 8588223715
e.mail: mmm-at-ucsd.edu
pager: 8586161420
e.mail: mmm-at-ucsd.edu
e.pager: 1620024619-at-alphapage.airtouch.com
www site: http://mil.ucsd.edu/
ftp site: mil.ucsd.edu





From daemon Mon Oct 9 09:38:40 2000



From: michael shaffer :      epmalab-at-darkwing.uoregon.edu
Date: Mon, 9 Oct 2000 07:16:21 -0700
Subject: RE: Options for Coolpix 990 to Zeiss (long)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Gary writes ...

} For storage, the CP990 uses CF type 1 media. The IBM
} microdrive will not work in this camera. Since the
} camera stores images in JPEG format, the file size of
} each image will vary according to how the image
} compresses. So far, the largest file I have taken is
} just over 1MB.

First ... thanx for compiling this info. However and regarding the
statement above ... are you sure the CP990 does not provide for saving
TIFFs? My CP800 will save a single high res TIFF to a 8Mb memory
card. Presumably one could save ~8-10 CP990 TIFFs to a 128Mb CF
card(?) Get back to us on this one ... I'm afraid "JPEG only" would
dissuade those who believe in "non-lossy" TIFFs only.

cheerios, =shAf= :o)

{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {}
Michael Shaffer, R.A. - mshaf-at-darkwing.uoregon.edu
Geological Science's Electron Probe Facility - University of Oregon
http://epmalab.uoregon.edu/





From daemon Mon Oct 9 10:12:37 2000



From: Kristen Lennon :      kalen-at-iastate.edu
Date: Mon, 09 Oct 2000 10:10:45 -0500
Subject: SeaLite info

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All,
Sorry to bother you with such a strange request. Does anyone have contact
information for SeaLite Sciences (associated with bioluminescent markers)?
All I have is a web site, and after several days of trying to get through,
I've just about given up.
Thanks for your help once again,
Kristen




From daemon Mon Oct 9 11:10:44 2000



From: John andrew :      johnpsdu-at-yahoo.com
Date: Mon, 9 Oct 2000 09:08:46 -0700 (PDT)
Subject: Sputter Coater for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I need to purchase a sputter coater for under 5K. Can
this group recomend what is a good purchase.

Thank you,
John

__________________________________________________
Do You Yahoo!?
Yahoo! Photos - 35mm Quality Prints, Now Get 15 Free!
http://photos.yahoo.com/



From daemon Mon Oct 9 11:15:16 2000



From: John andrew :      johnpsdu-at-yahoo.com
Date: Mon, 9 Oct 2000 09:12:30 -0700 (PDT)
Subject: Sputter Coater for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I need to purchase a sputter coater for under 5K. Can
this group recomend what would be a good purchase.

Thank you

__________________________________________________
Do You Yahoo!?
Yahoo! Photos - 35mm Quality Prints, Now Get 15 Free!
http://photos.yahoo.com/



From daemon Mon Oct 9 11:15:33 2000



From: Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 09 Oct 2000 08:09:40 -0700
Subject: RE: Options for Coolpix 990 to Zeiss (long)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 07:16 AM 10/9/00, you wrote:
} Gary writes ...
}
} } For storage, the CP990 uses CF type 1 media. The IBM
} } microdrive will not work in this camera. Since the
} } camera stores images in JPEG format, the file size of
} } each image will vary according to how the image
} } compresses. So far, the largest file I have taken is
} } just over 1MB.
}
} First ... thanx for compiling this info. However and regarding the
} statement above ... are you sure the CP990 does not provide for saving
} TIFFs? My CP800 will save a single high res TIFF to a 8Mb memory
} card. Presumably one could save ~8-10 CP990 TIFFs to a 128Mb CF
} card(?) Get back to us on this one ... I'm afraid "JPEG only" would
} dissuade those who believe in "non-lossy" TIFFs only.

Yes, it does have a TIFF mode. But this is only available via the
Manual mode. I figured that most folks wanted quick and easy
access to image capture. If they want/need TIFF, they will
have to attack the manual mode.

gary




From daemon Mon Oct 9 11:30:16 2000



From: Michael O'Keefe :      MAOKeefe-at-lbl.gov
Date: Mon, 09 Oct 2000 09:29:57 -0700
Subject: Re: TEM CCD camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Larry:
We would be very interested in hearing of what you come up with once you have a
solution.
Mike O'Keefe

Larry Allard wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Alan:
}
} Just this morning we were discussing the same problem with our Gatan
} CCD cameras on our HD-2000, one of which is retractable and used for
} TEM imaging, and the other of which is at the end of the GIF for
} energy-filtered imaging. These cameras are connected in series for
} the water flow, and have very tiny water lines which can easily clog,
} even with recirculated water. There is no provision by the
} manufacturer to provide for a safety cut-off of the Peltier cooler in
} the event of loss of water flow. We have recently experienced a
} burn-out of the TEM camera due to a clog in the line, which resulted
} in 2 weeks of downtime for repair (nicely done for free by Gatan),
} but still no response to queries about some sort of protection. So
} we have decided to find an appropriate water flow sensor, and to
} install it in an appropriate place in the water line, and to connect
} it to the camera controllers to provide a power cut-off when the
} water flow diminishes. I'll post the details of the solution when it
} is completed.
}
} Larry
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Dear Listers
} }
} } Have any other microscopists had problems with Peltier coolers failing on
} } water cooled CCD cameras?
} }
} } In my facility I have three such cameras all plumbed into the microscope
} } water system and connected, by the camera manufacturer, into wall sockets.
} } On one of the three cameras I have had the Peltier cooler fail four times
} } in two years. After the latest failure the manufacturer asked if the
} } camera had been run without water. I told him not intentionally but when
} } we have power cuts and the power is restored, the camera comes back on but
} } the microscope (and water) does not. The camera can therefore run for some
} } time without water cooling. Power failures are not uncommon at this
} } university!
} }
} } Many e-mails to the manufacturer have resulted in no response to my
} } questions about reliability of the Peltier coolers. The manufacturer is
} } treating the failure as an out of warranty issue but I believe there has
} } been something wrong with this camera since it was installed.
} }
} } Any experiences of listservers with Peltier cooler failures would be
} } appreciated. Please e-mail me directly.
} }
} } Regards
} }
} } Alan W Nicholls, PhD
} } Electron Microscopy Service Director
} } Research Resources Center - East (M/C 337)
} } Room 100 Science and Engineering South Building
} } The University of Illinois at Chicago
} } 845 West Taylor St
} } Chicago, IL 60607-7058
} }
} } Tel: 312 996 1227
} } Fax: 312 996 8091
} } Office: Room 110
} }
} } Web site www.rrc.uic.edu
} }
} }
} } Alan W Nicholls, PhD
} } Electron Microscopy Service Director
} } Research Resources Center - East (M/C 337)
} } Room 100 Science and Engineering South Building
} } The University of Illinois at Chicago
} } 845 West Taylor St
} } Chicago, IL 60607-7058
} }
} } Tel: 312 996 1227
} } Fax: 312 996 8091
} } Office: Room 110
} }
} } Web site www.rrc.uic.edu
}
} Dr. Lawrence F. Allard
} Senior Research Staff Member
} High Temperature Materials Laboratory
} Oak Ridge National Laboratory
} 1 Bethel Valley Road
} Bldg. 4515, MS 6064
} PO Box 2008
} Oak Ridge, TN 37831-6064
}
} 865-574-4981
} 865-576-5413 Fax
} allardlfjr-at-ornl.gov




From daemon Mon Oct 9 11:38:46 2000



From: Becky Holdford :      r-holdford-at-ti.com
Date: Mon, 09 Oct 2000 11:50:19 -0500
Subject: Texas Society for Microscopy 2000 Fall Meeting

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The 2000 Fall Meeting of the Texas Society for Microscopy will be held
on October 26, 27, and 28
at the DoubleTree Hotel in Dallas, TX. The hotel is located in
Campbell Centre at 8350 N. Central
Expressway, Dallas, TX 75206. The phone number for the hotel is
800-222-TREE.
A special workshop will be held on Thursday, Oct. 26, entitled "The
SEM Today". It will cover
the latest information concerning basic SEM, LVSEM, ESEM and
detectors.

For more information and/or to register, please contact
Pamela Neill, Program Chair TSM
Alcon Research LTD
6201 South Freeway
Forth Worth, TX 76134-2099
pamela.neill-at-alconlabs.com


--
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Becky Holdford (r-holdford-at-ti.com)
972-995-2360
KFAB Physical Analysis Lab--SEM/FIB/FA
Texas Instruments, Inc.
Dallas, TX
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~





From daemon Mon Oct 9 13:05:46 2000



From: Ladd Research :      ladres-at-worldnet.att.net
Date: Mon, 09 Oct 2000 14:03:01 -0400
Subject: Re: Sputter Coater for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear John,

We at Ladd may be able to help you. Please let us know what type of work
you want to do with it ((target, size of sample, type of sample prep,
etc) and I'll let you know what we have available.

Best Regards,

John Arnott


LADD RESEARCH
131 Dorset Lane
Williston, VT 05495 USA

web site http://www.laddresearch.com

TEL 1-800-451-3406 (US) or 1-802-878-6711 (anywhere)
FAX 1-802-878-8074
e-mail sales-at-laddresearch.com

Microscope Supplies Since 1955



John andrew wrote:
}
} ------------------------------------------------------------------------} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.}
} I need to purchase a sputter coater for under 5K. Can
} this group recomend what would be a good purchase.
}
} Thank you
}
} __________________________________________________
} Do You Yahoo!?
} Yahoo! Photos - 35mm Quality Prints, Now Get 15 Free!
} http://photos.yahoo.com/

--



From daemon Mon Oct 9 13:16:10 2000



From: Ladd Research :      ladres-at-worldnet.att.net
Date: Mon, 09 Oct 2000 14:14:06 -0400
Subject: Re: Carbon coated grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We appreciate the comments Dr. Joswaik and others concerning the quality
of Ladd coated grids.
Since there are still a number of researchers who coat their own grids,
we are pleased to share some of the things we have learned over the past
45 years.

1. Clean the evaporator at least once a day.
2. Change the diffusion pump oil at least every two weeks.
3. Inspect and clean each individual grid prior to coating.
4. Out gas the garbon prior to evaporation.
5. Use fresh chemicals with each batch of grids to be coated.

Hopefully some of this will be of assistance.
A recent posting mentioned that a diffusion pump system should be used
for carbon coating. We agree 100% with that. In fact we designed the
Ladd vacuum system with coated grids in mind by providing for "quick
changing" the oil and speedy clean-up.

John Arnott
--

LADD RESEARCH
131 Dorset Lane
Williston, VT 05495 USA

web site http://www.laddresearch.com

TEL 1-800-451-3406 (US) or 1-802-878-6711 (anywhere)
FAX 1-802-878-8074
e-mail sales-at-laddresearch.com

Microscope Supplies Since 1955



From daemon Mon Oct 9 13:18:56 2000



From: Mary Gail Engle :      mgengle-at-pop.uky.edu
Date: Mon, 09 Oct 2000 14:13:12 -0400
Subject: TEM for horse tendons

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear listers,
We received several VERY large pieces of horse tendon, half of which
was immersed in ethanol and the rest in formalin. The vet didn't know
which would be best , so that's how it arrived, via air from New
Mexico. We cut it into 1 x 3 mm pieces and processed it routinely, ie,
glut, OsO4, ethanol dehydration and PO. We infiltrated in Spurr's and PO
for 24 hrs, straight Spurr's for 2 hours and then embedded. What was left
just fell out of the blocks . Needless to say, we have a lot of tissue left
(still in EtOH and fomalin) and I was wondering if it's worth trying to
salvage and if anyone has any suggestions as to the best way to handle
this. I did ask the client to please contact me before sending any more
tissue but for now they would like us to try and get something from this.
Any ideas would really be appreciated.
Thank you,

Mary Gail Engle
Electron Microscopy & Imaging Facility
University of Kentucky



From daemon Mon Oct 9 13:43:39 2000



From: Dohnalkova, Alice :      Alice.Dohnalkova-at-pnl.gov
Date: Mon, 09 Oct 2000 11:40:49 -0700
Subject: Bacterial quantitation in soil samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




We are considering purchase of a system for quantitative analysis of bacterial
and fungal biovolume in soil samples. Could anyone provide info on equipment
and preferred method (FITC labeling?). As always, I appreciate your help,
Alice.

Alice Dohnalkova
Environmental Microbiology
Battelle, PNNL
MS P7-50
Richland, WA 99352
tel. (509) 372-0692
fax (509) 376-1321




From daemon Tue Oct 10 07:26:39 2000



From: Brian McIntyre :      mcintyre-at-optics.rochester.edu
Date: Tue, 10 Oct 2000 08:14:58 -0400
Subject: more info: saw-tooth scan distortion: jeol35

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


hi again-

before i go through the effort of separating the ground wire from the rest
of the supply system just a few more tidbits to throw out to the list:

1- the distortion in the scan seems to be about 60Hz (more cycles at
slower scan rates). in reduced area raster (about 4 frames/sec??) the
count is about 15. in "TV" (whatever rate that is on a 35) i get about 2
or 3 cycles.

2- the amplitude of the distortion increases a bit with decreasing KV
(i.e. its worse at 10KV than at 30KV...but not much worse). and it seems
to be worse at higher magnifications (at 10KX it just about covers the
screen left to right; at 500X it is just a couple of bumps).

3- i tightened all the ground lugs....no change.

4- i tightened all the console connectors...no change.

5- i moved the variac farther away from the console...no change.

6- perhaps unrelated.....the conformation of the "spot" realized when the
condenser lens current is decreased looks funny. i'm used to a realtively
uniform circular cross section. this one looks like a central uniform area
with dendritic "arms" radiating outwards...kinda like a spider web pattern.
does this mean anything to anyone?? (the final apertures are pretty
clean, and it looks funny with any of the three apertures)

thanks for all your previous comments...i'm sure to be closing in on fixing
this with your help.

brian
----------------------------------------------
Brian McIntyre
Electron Microscopy Lab- River Campus
Univ of Rochester
Rochester, NY 14627
716-275-3058/4875



From daemon Tue Oct 10 08:55:38 2000



From: Malis, Tom :      malis-at-NRCan.gc.ca
Date: Tue, 10 Oct 2000 09:51:32 -0400
Subject: RE: alternate fluid for ultramicrotomy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Scott. 'Bonne chance', as we say in Canada. A true water substitute (ie
in both knife edge wetability and surface tension) is one of those Holy
Grail things in ultramicrotomy. You could experiment with liquids like
alcohol mixtures, ethylene glycol, etc, but my suggestion is to save
yourself a lot of time and simply dry section. It's done more often than
you think; water-soluble minerals, reactive metals like Mg, to name a few.
Place a grid on the lower part of the diamond knife, section, then tease the
section onto the grid with your hair tool. An important point is to then
wet the section down onto the grid with something inert (perhaps an
alcohol), then dry under a lamp, so that it doesn't fall off the grid on
insertion into the TEM. If there is a danger of a somewhat brittle material
breaking apart upon sectioning, use a coated grid and some of the segments
should be retained.

Do let the Listserver know if someone out there suggests something that
works as well as water. You'll have a lot of friends at the next MSA
meeting!

Tom Malis
Group Leader - Characterization
Materials Technology Laboratory
Natural Resources Canada (Govt. of Canada)
568 Booth St., Ottawa, Canada
ph. 613-992-2310
FAX 613-992-8735
email: malis-at-nrcan.gc.ca



----------
From: Walck, Scott D. [SMTP:walck-at-ppg.com]
Sent: Friday, October 06, 2000 3:35 PM
To: 'Microscopy-at-MSA.Microscopy.Com'
Subject: alternate fluid for ultramicrotomy


------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html

-----------------------------------------------------------------------.


What other liquids can be used for floating off thin sections other
than
water? I have a sample that swells in water. It swells slowly in
alcohol.
Thanks in advance.

-Scott Walck




From daemon Tue Oct 10 10:43:38 2000



From: sghoshro-at-nmsu.edu
Date: Tue, 10 Oct 2000 09:39:16 -0600 (MDT)
Subject: bacteria protocol

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Just wanted to say thank you for the many replies to my TEM/SEM problem
with bacterial culture protocol. Problem solved, thanks again.
Kristine C. Fambrough
NMSU




From daemon Tue Oct 10 11:23:23 2000



From: Glen MacDonald :      glenmac-at-u.washington.edu
Date: Tue, 10 Oct 2000 09:25:01 +0100
Subject: Re: alternate fluid for ultramicrotomy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Many years ago, I was trying lipid retention embedding for TEM of
mycobacterium. GACH seemed to work but sectioning into water was a
nightmare. It attracted water from the trough during the
ultramicrotome's return stroke which then caused the block to swell.
Freon worked better, but the sample from DuPont was quite volatile and
evaporated quickly. The workaround was to section into water, but to
coat the sides of the block with the thinnest possible film of silicone
grease. The grease provided sufficient hydrophobicity to prevent water
from jumping onto the face, and if I worked quickly the sections could
be placed onto the grids. Needless to say, I only used a glass knife
for this. It was a short project, so column contamination from
volatiles in the grease wasn't a problem. Maybe a sample of the grease
could be put under a high vacuum beforehand to remove volatile
components.

Regards,
Glen

"Malis, Tom" wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hi Scott. 'Bonne chance', as we say in Canada. A true water substitute (ie
} in both knife edge wetability and surface tension) is one of those Holy
} Grail things in ultramicrotomy. You could experiment with liquids like
} alcohol mixtures, ethylene glycol, etc, but my suggestion is to save
} yourself a lot of time and simply dry section. It's done more often than
} you think; water-soluble minerals, reactive metals like Mg, to name a few.
} Place a grid on the lower part of the diamond knife, section, then tease the
} section onto the grid with your hair tool. An important point is to then
} wet the section down onto the grid with something inert (perhaps an
} alcohol), then dry under a lamp, so that it doesn't fall off the grid on
} insertion into the TEM. If there is a danger of a somewhat brittle material
} breaking apart upon sectioning, use a coated grid and some of the segments
} should be retained.
}
} Do let the Listserver know if someone out there suggests something that
} works as well as water. You'll have a lot of friends at the next MSA
} meeting!
}
} Tom Malis
} Group Leader - Characterization
} Materials Technology Laboratory
} Natural Resources Canada (Govt. of Canada)
} 568 Booth St., Ottawa, Canada
} ph. 613-992-2310
} FAX 613-992-8735
} email: malis-at-nrcan.gc.ca
}
} ----------
} From: Walck, Scott D. [SMTP:walck-at-ppg.com]
} Sent: Friday, October 06, 2000 3:35 PM
} To: 'Microscopy-at-MSA.Microscopy.Com'
} Subject: alternate fluid for ultramicrotomy
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
}
} -----------------------------------------------------------------------.
}
} What other liquids can be used for floating off thin sections other
} than
} water? I have a sample that swells in water. It swells slowly in
} alcohol.
} Thanks in advance.
}
} -Scott Walck
}

--

Glen MacDonald
Virginia Merrill Bloedel Hearing Research Center
Box 357923
University of Washington
Seattle, WA 98195-7923
glenmac-at-u.washington.edu
(206) 616-4156
(206) 616-1828 fax
************************************************************************
C:} The box said "Requires Windows95 or better". So I bought a Macintosh.
************************************************************************



From daemon Tue Oct 10 12:09:49 2000



From: O. O. Ilori :      sojilori-at-oauife.edu.ng
Date: Tue, 10 Oct 2000 18:09:12 +0100 (WAT)
Subject: SEM: Stub replacement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Guys
I am a novice user of a SEM (LEO 1450 to be precise) equipment which was
just installed in our central science lab.
Is there a way to clean stubs for reuse or are stubs disposable once used.
( This may sound naive)
soji
Mr. O. O. ILORI
DEPARTMENT OF ELECTRONIC/ELECTRICAL ENGINEERING
OBAFEMI AWOLOWO UNIVERSITY,
ILE-IFE, OSUN STATE
NIGERIA.
email: sojilori-at-oauife.edu.ng





From daemon Tue Oct 10 12:51:57 2000



From: Buckingham, Steve :      sbuckingham-at-excellatron.com
Date: Tue, 10 Oct 2000 13:48:56 -0400
Subject: JSM 840 users and Analysis contract labs in the Atlanta area

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All,
I'm tring to find users of JEOL JSM-840 (or similar) microscopes in
the Atlanta area. In addition, I need to find contract SEM analysis labs
here that might be able to run some fast turnaround samples for me (mostly
cross sections). I know several of you contacted me recently after I posted
for a microscopist, but I no longer have the information. Could you please
send mail again to sbuckingham-at-excellatron.com .
Many Thanks,
Steve

Steve Buckingham
Excellatron Solid State LLC
1460 Roswell Street, Suite J
Smyrna, GA 30080
phone 770 438 2201
fax 617 812 5920




From daemon Tue Oct 10 12:59:42 2000



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Tue, 10 Oct 2000 10:56:21 -0700
Subject: Re: more info: saw-tooth scan distortion: jeol35

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Brian,
The profile of the spot you describe sounds like an under-saturated filament.
At 08:14 AM 10/10/00 -0400, you wrote:

} hi again-
}
} before i go through the effort of separating the ground wire from the rest
} of the supply system just a few more tidbits to throw out to the list:
}
} 1- the distortion in the scan seems to be about 60Hz (more cycles at
} slower scan rates). in reduced area raster (about 4 frames/sec??) the
} count is about 15. in "TV" (whatever rate that is on a 35) i get about 2
} or 3 cycles.
}
} 2- the amplitude of the distortion increases a bit with decreasing KV
} (i.e. its worse at 10KV than at 30KV...but not much worse). and it seems
} to be worse at higher magnifications (at 10KX it just about covers the
} screen left to right; at 500X it is just a couple of bumps).
}
} 3- i tightened all the ground lugs....no change.
}
} 4- i tightened all the console connectors...no change.
}
} 5- i moved the variac farther away from the console...no change.
}
} 6- perhaps unrelated.....the conformation of the "spot" realized when the
} condenser lens current is decreased looks funny. i'm used to a realtively
} uniform circular cross section. this one looks like a central uniform area
} with dendritic "arms" radiating outwards...kinda like a spider web pattern.
} does this mean anything to anyone?? (the final apertures are pretty
} clean, and it looks funny with any of the three apertures)
}
} thanks for all your previous comments...i'm sure to be closing in on fixing
} this with your help.
}
} brian
} ----------------------------------------------
} Brian McIntyre

Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca




From daemon Tue Oct 10 15:23:33 2000



From: Tim Lyden :      lyden.11-at-osu.edu
Date: Tue, 10 Oct 2000 16:31:28 +0100
Subject: What's up...

Contents Retrieved from Microscopy Listserver Archives
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Where is everyone.... I have not gotten a posting since yesterday????




From daemon Tue Oct 10 17:11:15 2000



From: michael shaffer :      epmalab-at-darkwing.uoregon.edu
Date: Tue, 10 Oct 2000 15:09:14 -0700
Subject: RE: more info: saw-tooth scan distortion: jeol35

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Brian writes ...

} ...
}
} 1- the distortion in the scan seems to be about 60Hz

This might imply you have a ground loop problem. Possibly one of the
other devices (computer, EDX, ...) is providing a ground connection
independent of your primary ground(?)

cheerios, =shAf= :o)

{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {}
Michael Shaffer, R.A. - mshaf-at-darkwing.uoregon.edu
Geological Science's Electron Probe Facility - University of Oregon
http://epmalab.uoregon.edu/





From daemon Tue Oct 10 17:42:44 2000



From: Dee Breger :      micro-at-ldeo.columbia.edu
Date: Tue, 10 Oct 2000 18:40:31 -0400 (EDT)
Subject: digital imaging info needed

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)( / _ Jonathan Lipkin
() \ _ Assistant Professor of Multimedia Design
)( / _ Ramapo College of New Jersey
() \ _ http://arts.ramapo.edu/lipkin
)( / _ 201/ 684/ 7056/

Dear listserv:

Dee Breger was kind enough to forward this message for me. I'm writing a book
on digital photography, to be published by Harry N. Abrams next year. The book
will include a section on applied digital imaging, and I am particularly
interested in scientific imaging. I am hoping that some of you folks might
be able
to provide some information.

For the purposes of the book, I am defining digital imaging as that which
describes a scene using discrete digital information in a raster grid, or
bitmap. So, I am particularly interested in scientific imaging applications
which gather information in this way.

I would be interested to hear if there are other scientific imaging systems
(DEE: besides SEMs) which collect information in this manner. For instance,
it sounds as though atomic force microscopes work in this way: as they
drag a stylus across a surface, the position is recorded (x and y), along
with the height (z). This
is later used to re-create the image in a raster grid (the fact that the AFM
does not use light makes it another interesting foil to 'traditional' digital
photography). How then, are colors in the final output determined from the
data collected? I would also, of course, love to see any images you might have
to show.

Thanks in advance,
Jonathan Lipkin (jlipkin-at-ramapo.edu)

DEE: I'll forward to Jonathan any replies that are publically posted to the
listserver. He also needs other detailed technical information on digital
image formation which can be far more comprehensively clarified and
amplified by some of the experts among my fellow listers than by me, though
I've already given him the broad picture for analog scopes like my dear old
Cambridge 250. His SEM impressions, which I'll pass along verbatim,
include (1) "SEMs collect information, used to fill a raster grid, through
the use of a PMT", (2) the signal is sent directly to the crt in analog
form -I always had thought that the pmt sent out digital info, but it
sounds like it just sends out analog data (a waveform? though it couldn't
be a waveform if the raster grid is discrete in two dimensions)" and (3)
"as the secondary electrons come off the sample, they hit a detector which
generates a digital number (redundant) which is then placed in a pixel of a
bitmap."

Many thanks to any who can help!





***************************************************************
Dee Breger
Mgr. SEM/EDX Facility
Lamont-Doherty Earth Observatory
61 Route 9W
Palisades, NY 10964 USA
T: 914/365-8640
F: 914/365-8155

http://www.ldeo.columbia.edu/micro
http://www.discovery.com/area/science/micro/micro1.html
http://www.lsc.org/antarctica/front.html
Journeys in Microspace (Columbia University Press, 1995)





From daemon Tue Oct 10 17:55:38 2000



From: L. D. Marks :      ldm-at-risc4.numis.nwu.edu
Date: Tue, 10 Oct 2000 18:09:54 -0500 (CDT)
Subject: Ultrasonic Disc Cutter

Contents Retrieved from Microscopy Listserver Archives
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Does anyone in the Chicago area have an ultrasonic
disc cutter we can use temporarily? Alternatively,
does anyone have one they are not using and would
be willing to sell or donate?

++++++++++++++++++++++++++++++++++++++++++++++++
Laurence Marks
Department of Materials Science and Engineering
Northwestern University
Tel: (847) 491-3996 Fax: (847) 491-7820
mailto:ldm-at-risc4.numis.nwu.edu
http://www.numis.nwu.edu
++++++++++++++++++++++++++++++++++++++++++++++++




From daemon Tue Oct 10 18:09:20 2000



From: David Henriks :      Henriks-at-CompuServe.COM
Date: Tue, 10 Oct 2000 19:07:23 -0400
Subject: Ultrasonic Disc Cutter

Contents Retrieved from Microscopy Listserver Archives
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Dear Dr. Marks:

If you aren't able to find an ultrasonic cutter locally to use, please let
me know. Perhaps I can arrange to have one of our SoniCut 380 systems sent
from our applications lab for short term use or maybe we could arrange to
cut some samples for you here. Please let me know if I can be of help.

Best regards-

David
Writing at 3:46:01 PM on 10/10/2000

***************************************************************************
************************

David Henriks
Vice President TEL: 800-728-2233
(toll free in the USA)
South Bay Technology, Inc. +1-949-492-2600
1120 Via Callejon FAX: +1-949-492-1499
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

***************************************************************************
************************

} } } } } Please visit us at http://www.southbaytech.com { { { { {

Manufacturers of precision sample preparation equipment and supplies for
metallography, crystallography and electron microscopy.

Message text written by "L. D. Marks"
}
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Does anyone in the Chicago area have an ultrasonic
disc cutter we can use temporarily? Alternatively,
does anyone have one they are not using and would
be willing to sell or donate?

++++++++++++++++++++++++++++++++++++++++++++++++
Laurence Marks
Department of Materials Science and Engineering
Northwestern University
Tel: (847) 491-3996 Fax: (847) 491-7820
mailto:ldm-at-risc4.numis.nwu.edu
http://www.numis.nwu.edu
++++++++++++++++++++++++++++++++++++++++++++++++

{



From daemon Tue Oct 10 18:53:32 2000



From: Rick Harris :      raharris-at-ucdavis.edu
Date: Tue, 10 Oct 2000 16:49:43 -0700
Subject: bell jar treatment

Contents Retrieved from Microscopy Listserver Archives
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Well we've got a new vacuum evaporator and it has been so long since I've
had one I've forgotten how to treat the bell jar to reduce the effort it
takes to keep it clean. However, I do recall that there were little tricks
to cleaning and treating the glass and other surfaces. Anyone care to shed
a little light my darkened memory of these procedures?

TIA

Rick A. Harris, Director
Microscopy and Imaging Facility
Section of Molecular and Cellular Biology
1241 Life Sciences Addition
University of California
Davis, CA
530 752 2914
530 754 7536 fax
http://katie.ucdavis.edu
raharris-at-ucdavis.edu




From daemon Tue Oct 10 19:14:59 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Tue, 10 Oct 2000 19:05:05 -0500
Subject: Administrivia: Subscriber List Restored

Contents Retrieved from Microscopy Listserver Archives
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G'day All...

Another fault this weekend, this time the subscription list
became corrupted. This mean't a significant number of you
(~ 1500 ) "disappeared" for a few days and hence did not receive mail.
Iunfortunatley did not notice since the mail did go through to the rest
of the subscribers on the list. In any event , I have restored the list to
it's Oct 6th backup copy.

To those of you that unsubscribed between Oct 6th and
Oct 10th. I apologize and ask you to unsubscribe again.
I, unfortunately, do not keep records of those of you that
have left.

Sorry...

Nestor
Your Friendly Neighborhood SysOp





From daemon Tue Oct 10 21:19:14 2000



From: NGUYEN THI VAN ANH :      vasnf-at-biochem.tohoku.ac.jp
Date: Wed, 11 Oct 2000 11:15:24 +0900
Subject: Cell adhesion

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Dear all,

I am trying to get the internal(cytoplasmic) side of a prepared erythrocyte
ghost's plasma membrane attached to coverslip, so that the external side is free
in water solution. Does anyone have an experience with this or suggest the
reagent that preferably interacts with inner leaflet of plasma membrane or
cytoskeletal protein ? How should I test the external side is free ?

Any suggestions on this problem would be greatly welcomed. Thank you

Nguyen

Lab. of Appl. Microbiology
Dep. of Biochemistry
Faculty of Agriculture
Tohoku University
Sendai, Japan.







From daemon Tue Oct 10 22:02:17 2000



From: John Nailon :      mmjnailo-at-dingo.cc.uq.edu.au
Date: Wed, 11 Oct 2000 12:55:04 +1000
Subject: Re: bell jar treatment

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The easiest way to keep your bell jar clean is to wipe a thin film of
detergent over the surface of the clean glass and pump down to dry.
After each coating run simply rinse off the glass, the detergent comes
away and takes the coating with it. Recoat with detergent and dry.

Train ALL users in this technique, insist they clean the glass if they
"FORGOT" to do it after their last run, and you will always have a
clear, clean bell jar.
Regards
JVN
Rick Harris wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Well we've got a new vacuum evaporator and it has been so long since I've
} had one I've forgotten how to treat the bell jar to reduce the effort it
} takes to keep it clean. However, I do recall that there were little tricks
} to cleaning and treating the glass and other surfaces. Anyone care to shed
} a little light my darkened memory of these procedures?
}
} TIA
}
} Rick A. Harris, Director
} Microscopy and Imaging Facility
} Section of Molecular and Cellular Biology
} 1241 Life Sciences Addition
} University of California
} Davis, CA
} 530 752 2914
} 530 754 7536 fax
} http://katie.ucdavis.edu
} raharris-at-ucdavis.edu

--
****************************************************
John V Nailon
Operations Manager
Centre for Microscopy and Microanalysis
The University of Queensland
St. Lucia Queensland 4072
Phone: +61-7-3365-4214
Fax: +61-7-3365-4422
WWW: http://www.uq.edu.au/nanoworld/allstaff.html#Nailon
****************************************************



From daemon Tue Oct 10 22:06:21 2000



From: Ben Craft :      bcraft-at-uci.edu
Date: Tue, 10 Oct 2000 20:04:42 -0700 (PDT)
Subject: High Resolution SEM

Contents Retrieved from Microscopy Listserver Archives
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I'm getting ready to purchase a new sputter coater for our FESEM. I'd
like to hear how lucky others were with using a "regular" sputter coater
for high resolution SEM work. Currently, there is a noise problem with
our SEM, so the resolution is about 3-4nm. I've successfully imaged 5nm
ZrO2 close-packed particles, and 4nm pores in glass using a thin coating
of Au/Pd applied with an old Polaron. One thing I'm worried about is =
once the noise is fixed and I can resolve the published 1.5nm resolution
will I be able to resolve the grains in the coating?

Thanks,
Ben



#######
#####\_O -Ben Craft-
####/\/}
#### /"
### \






From daemon Tue Oct 10 23:06:07 2000



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Wed, 11 Oct 2000 00:06:57 -0500
Subject: Bell jar maintenance

Contents Retrieved from Microscopy Listserver Archives
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-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Rick A. Harris wrote:
===============================================================
Well we've got a new vacuum evaporator and it has been so long since I've
had one I've forgotten how to treat the bell jar to reduce the effort it
takes to keep it clean. However, I do recall that there were little tricks
to cleaning and treating the glass and other surfaces. Anyone care to shed
a little light my darkened memory of these procedures?
===============================================================
One product that will do this is called Bell Bright™. A companion product
for metal surfaces is called Metal Bright™. Both are described in detail
on the SPI website at URL
http://www.2spi.com/catalog/supp/supp3.html

The concept is to leave a thin water soluble polymer behind on the clean
glass (or metal) surface, and then when it is time to remove the evaporated
deposited, a lint free cotton wiper and water alone will wipe away most of
the material (as the originally deposited polymer film dissolves away).

Disclaimer: SPI Supplies "invented" this product in the late 1970's and has
offered it ever since. We would have a vested interest in seeing its use
increase.


Chuck


PS: Remember that we are trying to become 100% paperless and the only way
that we can manage this kind of correspondence, in a paperless environment,
is for our correspondents to always reply by way of "reply" on their
software so that the entire string of correspondence on that topic gets
returned to us, and the entire correspondence history can be kept in one
place.

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.spi.cc
########################
============================================



From daemon Wed Oct 11 00:24:27 2000



From: Chris Baker :      chris-at-collegewafer.com
Date: Wed, 11 Oct 2000 01:26:19 -0700
Subject: Si, MEMS, GaAs, SOI, InP, GaN, Ge, PtSi, SOI & Equipment..

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Visit http://www.collegewafer.com for your free quote, or call (800) 713-9375
or
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To REMOVE yourself from this list please visit
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MEMS6730





From daemon Wed Oct 11 05:51:12 2000



From: Emma Bonino :      emma.bonino-at-elezioniradicali.padova.com
Date: Wed, 11 Oct 2000 11:25:33 +0200
Subject: Toc toc. Sono Emma Bonino e la prego...

Contents Retrieved from Microscopy Listserver Archives
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Toc toc. Mi scusi, sono Emma Bonino e la prego di partecipare al gioco
online che stiamo organizzando perche' anche cittadini come lei (che non
votino affatto radicale o che lo facciano, che non votino o non intendano
piu' votare) possano partecipare alle nostre decisioni, essere presenti o
rappresentati nei nostri organi dirigenti.

In vista delle elezioni politiche occorre tentare di allearsi con il Polo o
con l'Ulivo? O combattere da soli? O organizzare il boicottaggio di elezioni
non democratiche? Quali Riforme istituzionali, politiche, del lavoro,
dell'impresa: "americane" o "tedesche", o nessuna? Quali politiche sulle
liberta' individuali e sulla scienza, oltre che sulle droghe: proibizioniste
o antiproibizioniste, come gia' sul divorzio e sull'aborto? E i partiti
devono aprirsi a tutti i cittadini attraverso Internet, o restare "chiusi"
come finora?

Dal 27 novembre al 3 dicembre si votera' online per eleggere 25 nuovi membri
del Comitato Radicale. Se, come vivamente spero, lei intende partecipare a
questo gioco, lo preannunci e registri cliccando qui:
http://www.radicali.it/p_register/

Mi scusi ancora. A presto.

Emma Bonino

PS Questa email e' stata inviata nel rispetto della legge
sulla privacy; se desidera maggiori informazioni o se intende
cancellarsi, puo' cliccare qui: http://www.radicali.it/p_privacy_com3/




From daemon Wed Oct 11 05:52:10 2000



From: Emma Bonino :      emma.bonino-at-elezioniradicali.padova.com
Date: Wed, 11 Oct 2000 11:25:33 +0200
Subject: Toc toc. Sono Emma Bonino e la prego...

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Toc toc. Mi scusi, sono Emma Bonino e la prego di partecipare al gioco
online che stiamo organizzando perche' anche cittadini come lei (che non
votino affatto radicale o che lo facciano, che non votino o non intendano
piu' votare) possano partecipare alle nostre decisioni, essere presenti o
rappresentati nei nostri organi dirigenti.

In vista delle elezioni politiche occorre tentare di allearsi con il Polo o
con l'Ulivo? O combattere da soli? O organizzare il boicottaggio di elezioni
non democratiche? Quali Riforme istituzionali, politiche, del lavoro,
dell'impresa: "americane" o "tedesche", o nessuna? Quali politiche sulle
liberta' individuali e sulla scienza, oltre che sulle droghe: proibizioniste
o antiproibizioniste, come gia' sul divorzio e sull'aborto? E i partiti
devono aprirsi a tutti i cittadini attraverso Internet, o restare "chiusi"
come finora?

Dal 27 novembre al 3 dicembre si votera' online per eleggere 25 nuovi membri
del Comitato Radicale. Se, come vivamente spero, lei intende partecipare a
questo gioco, lo preannunci e registri cliccando qui:
http://www.radicali.it/p_register/

Mi scusi ancora. A presto.

Emma Bonino

PS Questa email e' stata inviata nel rispetto della legge
sulla privacy; se desidera maggiori informazioni o se intende
cancellarsi, puo' cliccare qui: http://www.radicali.it/p_privacy_com3/




From daemon Wed Oct 11 06:21:17 2000



From: bk22k-at-fiberia.com
Date: Wed, 11 Oct 00 06:04:50 EST
Subject: opportunity knocks-are you home?

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"RECEIVE at least 20 ORDERS FOR REPORT #1 WITHIN 2 WEEKS. IF
YOU DON'T, SEND OUT MORE PROGRAMS UNTIL YOU DO!" My first
step in making $50,000 in 90 days was done. By January 30, I had received
196 orders for REPORT #2. Your goal is to "RECEIVE AT LEAST 100+ ORDERS
FOR REPORT #2 WITHIN 2 WEEKS. IF NOT, SEND OUT MORE PROGRAMS
UNTIL YOU DO. ONCE YOU HAVE 100 ORDERS,
THE REST IS EASY, RELAX, YOU WILL MAKE YOUR $50,000 GOAL." Well, I
had 196 orders for REPORT #2, 96 more than I needed. So I sat back
and relaxed. By March 1, of my e-mailing of 10,000, I received $58,000 with
more coming in every day.

I paid off ALL my debts and bought a much needed new car. Please take
time to read the attached program, IT WILL CHANGE YOUR LIFE FOREVER!!
! Remember, it won't work if you don't try it. This program does work
, but you must follow it EXACTLY! Especially the rules of not trying
to place your name in a different place. It won't work and you'll
lose out on a lot of money!
In order for this program to work, you must meet your goal of 20+
orders for REPORT #1, and 100+ orders for REPORT #2 and you will make $50,000
or more in 90 days. I AM LIVING PROOF THAT IT WORKS!!!

If you choose not to participate in this program, I am sorry. It
really is a great opportunity with little cost or risk to you. If you
choose to participate, follow the program and you will be on your way
to financial security. If you are a fellow business owner and are in
financial trouble like I was, or you want to start your own business, consider
this a sign. I DID!

Sincerely,
Johnathon Rourke

A PERSONAL NOTE FROM THE ORIGINATOR OF THIS PROGRAM:

By the time you have read the enclosed program and reports, you
should have concluded that such a program, and one that is legal,
could not have been created by an amateur.

Let me tell you a little about myself. I had a profitable business
for 10 years. Then in 1979 my business began falling off. I was doing
the same things that were previously successful for me, but it wasn't
working. Finally, I figured it out. It wasn't me, it was the economy.
Inflation and recession had replaced the stable economy that had been
with us since 1945.I don't have to tell you what happened to the
unemployment rate... because many of you know from first hand
experience. There were more failures and bankruptcies than ever before.

The middle class was vanishing. Those who knew what they were doing
invested wisely and moved up. Those who did not, including those who
never had anything to save or invest, were moving down into the ranks
of the poor. As the saying goes, "THE RICH GET RICHER AND THE POOR
GET POORER." The traditional methods of making money will never allow
you to "move up" or "get rich", inflation will see to that.

You have just received information that can give you financial
freedom for the rest of your life, with "NO RISK" and "JUST A LITTLE
BIT OF EFFORT." You can make more money in the next few months than you
have ever imagined. I should also point out that I will not see a penny of this
money, nor anyone else who has provided a testimonial for this
program. I have already made over 4 MILLION DOLLARS!I have retired
from the program after sending thousands and thousands of programs.

Follow the program EXACTLY AS INSTRUCTED. Do not change it in any way
. It works exceedingly well as it is now. Remember to e-mail a copy
of this exciting report to everyone you can think of. One of the
people you send this to may send out 50,000...and your name will be on everyone of
them!

Remember though, the more you send out the more potential customers
you will reach.

So my friend, I have given you the ideas, information, materials and
opportunity to become financially independent. IT IS UP TO YOU NOW!

"THINK ABOUT IT"

Before you delete this program from your mailbox, as I almost did,
take a little time to read it and REALLY THINK ABOUT IT. Get a pencil
and figure out what could happen when YOU participate. Figure out the
worst possible response and no matter how you calculate it, you will
still make a lot of money! You will definitely get back what you
invested. Any doubts you have will vanish when your first orders come
in. IT WORKS!

Jody Jacobs, Richmond, VA

HERE'S HOW THIS AMAZING PROGRAM WILL MAKE YOU THOUSANDS OF
DOLLAR$

INSTRUCTIONS:

This method of raising capital REALLY WORKS 100% EVERY TIME.
I am sure that you could use up to $50,000 or more in the next 90
days. Before you say "BULL... ", please read this program carefully.

This is not a chain letter, but a perfectly legal money making
opportunity. Basically, this is what you do: As with all multi-level
businesses, we build our business by recruiting new partners and
selling our products. Every state in the USA allows you to recruit
new multi-level business partners,
and we offer a product for EVERY dollar sent. YOUR ORDERS COME BY
MAIL AND ARE FILLED BY E-MAIL, so you are not involved in personal
selling. You do it privately in your own home, store or office. This
is the GREATEST Multi-Level Mail Order Marketing anywhere.

This is what you MUST do:

1. Order all 4 reports shown on the list below (you can't sell them
if youdon't order them).
-- For each report, send $5.00 CASH, the NAME & NUMBER OF THE REPORT
YOU ARE ORDERING, YOUR E-MAIL ADDRESS, and YOUR NAME & RETURN
ADDRESS (in case of a problem) to the person whose name appears on
the list next to the report. MAKE SURE YOUR RETURN ADDRESS IS ON
YOUR ENVELOPE IN CASE OF ANY MAIL PROBLEMS!
-- When you place your order, make sure you order each of the four
reports. You will need all four reports so that you can save them on
your computer and resell them.
-- Within a few days you will receive, via e-mail, each of the four
reports. Save them on your computer so they will be accessible for you to send
to the 1,000's of people who will order them from you.

2. IMPORTANT DO NOT alter the names of the people who are listed next
to each report, or their sequence on the list, in any way other than
is instructed below in steps "a" through "f" or you will lose out on
the majority of your profits. Once you understand the way this works,
you'll also see how it doesn't work if you change it. Remember, this
method has been tested,and if you alter it, it will not work.
a. Look below for the listing of available reports.
b. After you've ordered the four reports, take this advertisement and
remove the name and address under REPORT #4. This person has
made it through the cycle and is no doubt counting their $50,000!
c. Move the name and address under REPORT #3 down to REPORT #4.
d. Move the name and address under REPORT #2 down to REPORT #3.
e. Move the name and address under REPORT #1 down to REPORT #2.
f. Insert your name/address in the REPORT #1 position.

Please make sure you COPY ALL INFORMATION, every name and
address,
ACCURATELY!

3. Take this entire letter, including the modified list of names, and
save it to your computer. Make NO changes to the instruction portion
of this letter.

Your cost to participate in this is practically nothing (surely
you can afford $20). You obviously already have an Internet
connection and e-mail is FREE!



There are two primary methods of building your downline:

METHOD #1: SENDING BULK E-MAIL

Let's say that you decide to start small, just to see how it goes,
and we'll assume you and all those involved send out only 2,000
programs each. Let's also assume that the mailing receives a 0.5%
response. Using a good list the response could be much better. Also,
many people will send out hundreds of
thousands of programs instead of 2,000. But continuing with this
example, you send out only 2,000 programs. With a 0.5% response, that
is only 10 orders for REPORT #1. Those 10 people respond by sending
out 2,000 programs each for a total of 20,000. Out of those 0.5%, 100
people respond and order REPORT #2. Those 100 mail out 2,000 programs
each for a total of 200,000.
The 0.5% response to that is 1,000 orders for REPORT #3. Those 1,000
send out 2,000 programs each for a 2,000,000 total. The 0.5% response
to that is 10,000 orders for REPORT #4. That's 10,000 $5 bills for
you. CASH!!! Your total income in this example is $50 + $500 + $5,000
+ $50,000 for a total of
$55,550!!! REMEMBER FRIEND, THIS IS ASSUMING 1,990 OUT OF THE 2,000
PEOPLE YOU MAIL TO WILL DO ABSOLUTELY NOTHING AND TRASH THIS
PROGRAM! DARE TO THINK FOR A MOMENT WHAT WOULD HAPPEN IF
EVERYONE, OR HALF SENT OUT 100,000 PROGRAMS INSTEAD OF 2,000.
Believe me, many people will do justthat, and more! By the way, your cost to
participate in this is practically nothing. You obviously already have an Internet
connection and e-mail is FREE!!! REPORT #2 will show you the best
methods for bulk e-mailing, tell you where
to obtain free bulk e-mail software and where to obtain e-mail lists.



METHOD #2 - PLACING FREE ADS ON THE INTERNET

Advertising on the internet is very, very inexpensive, and there are
HUNDREDS of FREE places to advertise. Let's say you decide to start
small just to see how well it works. Assume your goal is to get ONLY
10 people to participate on your first level. (Placing a lot of FREE
ads on the Internet will EASILY get a larger response.) Also assume that
everyone else in YOUR ORGANIZATION gets ONLY 10 downline members.
Follow this example to achieve the STAGGERING results below:

1st level--your 10 members with $5.......................................$50
2nd level--10 members from those 10 ($5 x 100)..................$500
3rd level--10 members from those 100 ($5 x 1,000)...........$5,000
4th level--10 members from those 1,000 ($5 x 10,000).....$50,000
THIS TOTALS ----------} $55,550

Remember friends, this assumes that the people who participate only
recruit 10 people each. Think for a moment what would happen if they
got 20 people to participate! Most people get 100's of participants!
THINK ABOUT IT! For every $5.00 you receive, all you must do is e-mail them
the report they ordered. THAT'S IT! ALWAYS PROVIDE SAME-DAY SERVICE
ON ALL ORDERS! This will guarantee that the e-mail THEY send out with YOUR
name and address on it will be prompt because they can't advertise
until they receive the report!

AVAILABLE REPORTS

*** Order Each REPORT by NUMBER and NAME ***
Notes:
-- ALWAYS SEND $5 CASH (U.S. CURRENCY) FOR EACH REPORT. CHECKS NOT
ACCEPTED.
-- ALWAYS SEND YOUR ORDER VIA FIRST CLASS MAIL.
-- Make sure the cash is concealed by wrapping it in at least two
sheets of paper. On one of those sheets of paper, include:
(a) the number & name of the report you are ordering, (b) your
e-mail address, and (c) your name & postal address.

PLACE YOUR ORDER FOR THESE REPORTS NOW:


REPORT #1 "The Insider's Guide to Advertising for Free on the
Internet"


ORDER REPORT #1 FROM:

D. Baggett 316 gh lowery rd Chatsworth, Ga. 30705

REPORT #2 "The Insider's Guide to Sending Bulk E-mail on the Internet"

ORDER REPORT #2 FROM:

A. Picciotti 3202 The Highlands Drive Sugarland, Tx 77478

REPORT #3 "The Secrets of Multilevel Marketing on the Internet"

ORDER REPORT #3 FROM:

L. Daehlin 3208 Wellington Ct. Suite P Raliegh, N C 27615

REPORT #4 "How to Become a Millionaire Utilizing the Power of Multilevel
Marketing and the Internet"

ORDER REPORT #4 FROM:

P. Drucker 1517 A Rolling Glen Rd Boothwyn, Pa. 19061

About 50,000 new people get online every month!

******* TIPS FOR SUCCESS *******
-- TREAT THIS AS YOUR BUSINESS! Be prompt, professional, and follow
the directions accurately.
-- Send for the four reports IMMEDIATELY so you will have them when
the orders start coming in because: When you receive a $5 order, you
MUST send out the requested product/report.
-- ALWAYS PROVIDE SAME-DAY SERVICE ON THE ORDERS YOU RECEIVE.
-- Be patient and persistent with this program. If you follow the
instructions exactly, your results WILL BE SUCCESSFUL!
-- ABOVE ALL, HAVE FAITH IN YOURSELF AND KNOW YOU WILL SUCCEED!

******* YOUR SUCCESS GUIDELINES *******
Follow these guidelines to guarantee your success:

If you don't receive 20 orders for REPORT #1 within two weeks,
continue

advertising or sending e-mails until you do. Then, a couple of weeks
later you should receive at least 100 orders for REPORT#2. If you don
't, continue advertising or sending e-mails until you do. Once you
have received 100 or more orders for REPORT #2, YOU CAN RELAX,
because the system is already working for you, and the cash will
continue to roll in!

THIS IS IMPORTANT TO REMEMBER:
Every time your name is moved down on the list, you are placed in
front of a DIFFERENT report. You can KEEP TRACK of your PROGRESS by
watching which report people are ordering from you. If you want to
generate more income, send another batch of e-mails or continue
placing ads and start the whole process again! There is no limit to
the income you will generate from this business!

Before you make your decision as to whether or not you participate in
this program. Please answer one question. DO YOU WANT TO CHANGE YOUR
LIFE? If the answer is yes, please look at the following facts about
this program:

1. You are selling a product which does not Cost anything to PRODUCE,
SHIP OR ADVERTISE.
2. All of your customers pay you in CASH!
3. E-mail is without question the most powerful method of
distributing information on earth. This program combines the
distribution power of e-mail together with the revenue generating
power of multi-level marketing.
4. Your only expense--other than your initial $20 investment--is your
time!
5. Virtually all of the income you generate from this program is PURE
PROFIT!
6. This program will change your LIFE FOREVER.

ACT NOW!Take your first step toward achieving financial independence.
Orderthe reports and follow the program outlined above--SUCCESSwill
be yourreward.

Thank you for your time and consideration.

PLEASE NOTE: If you need help with starting a business, registering a
business name, learning how income tax is handled, etc., contact your
localoffice of the Small Business Administration (a Federal Agency)
1-800-827-5722 for free help and answers to questions. Also, the
InternalRevenue Service offers free help via telephone and free
seminars aboutbusiness tax requirements. Your earnings are highly
dependant on youractivities and advertising. The information
contained on this site and in the report constitutes no guarantees
stated nor implied. In the event that it is determined that this site
or report constitutes a guarantee of any kind, that guarantee is now
void. The earnings amounts listed on this site and in the report are
estimates only. If you have any questions of the legality of this
program, contact the Office of Associate Director for Marketing
Practices, Federal Trade Commission, Bureau of Consumer Protection in
Washington, DC.


/////////////////////////////////////////////////////////////////
Remove at qmkx-at-netscape.net
/////////////////////////////////////////////////////////////////










From daemon Wed Oct 11 06:29:10 2000



From: Emma Bonino :      emma.bonino-at-elezioniradicali.padova.com
Date: Wed, 11 Oct 2000 11:25:33 +0200
Subject: Toc toc. Sono Emma Bonino e la prego...

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Wed, 11 Oct 2000 11:22:01 +0200
Message-ID: {001401c03365$51216370$2c9b83d4-at-pol.it}
Reply-To: "Emma Bonino" {emma.bonino-at-elezioniradicali.padova.com}


Toc toc. Mi scusi, sono Emma Bonino e la prego di partecipare al gioco
online che stiamo organizzando perche' anche cittadini come lei (che non
votino affatto radicale o che lo facciano, che non votino o non intendano
piu' votare) possano partecipare alle nostre decisioni, essere presenti o
rappresentati nei nostri organi dirigenti.

In vista delle elezioni politiche occorre tentare di allearsi con il Polo o
con l'Ulivo? O combattere da soli? O organizzare il boicottaggio di elezioni
non democratiche? Quali Riforme istituzionali, politiche, del lavoro,
dell'impresa: "americane" o "tedesche", o nessuna? Quali politiche sulle
liberta' individuali e sulla scienza, oltre che sulle droghe: proibizioniste
o antiproibizioniste, come gia' sul divorzio e sull'aborto? E i partiti
devono aprirsi a tutti i cittadini attraverso Internet, o restare "chiusi"
come finora?

Dal 27 novembre al 3 dicembre si votera' online per eleggere 25 nuovi membri
del Comitato Radicale. Se, come vivamente spero, lei intende partecipare a
questo gioco, lo preannunci e registri cliccando qui:
http://www.radicali.it/p_register/

Mi scusi ancora. A presto.

Emma Bonino

PS Questa email e' stata inviata nel rispetto della legge
sulla privacy; se desidera maggiori informazioni o se intende
cancellarsi, puo' cliccare qui: http://www.radicali.it/p_privacy_com3/




From daemon Wed Oct 11 06:39:50 2000



From: Jim at ProSciTech :      jim-at-proscitech.com
Date: Wed, 11 Oct 2000 21:09:07 +1000
Subject: RE: Stub replacement

Contents Retrieved from Microscopy Listserver Archives
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It all depends: how busy you are, how expensive staff time in your country is
and how costly the place is you buy your stubs from. Obviously some specimens
should be stored in a desiccator for an eternity and other can be discarded
immediately.
There is nothing magical about cleaning stubs. Solvents are messy. Best to
place on some newspaper a very coarse file or a sheet of coarse (50 grit)
sandpaper and then rub the old stub over that file of sandpaper until specimen
and glue are removed.

Disclaimer: ProSciTech sells stubs, but we are happy if people recycle and this
leaves some money for useful purchases.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Wednesday, October 11, 2000 3:09 AM, O. O. Ilori
[SMTP:sojilori-at-oauife.edu.ng] wrote:
}
} Hello Guys
} I am a novice user of a SEM (LEO 1450 to be precise) equipment which was
} just installed in our central science lab.
} Is there a way to clean stubs for reuse or are stubs disposable once used.
} ( This may sound naive)
} soji
} Mr. O. O. ILORI
} DEPARTMENT OF ELECTRONIC/ELECTRICAL ENGINEERING
} OBAFEMI AWOLOWO UNIVERSITY,
} ILE-IFE, OSUN STATE
} NIGERIA.
} email: sojilori-at-oauife.edu.ng
}
}




From daemon Wed Oct 11 07:33:25 2000



From: Rosemary Walsh :      rw9-at-psu.edu
Date: Wed, 11 Oct 2000 08:57:04 -0400
Subject: Inquiry re: assessment of critical point dryers

Contents Retrieved from Microscopy Listserver Archives
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Dear Listers,
The following are responses to my inquiry about
automated critical point dryers. There was one other which
I will post as well. Thanks to all who responded.
Rosemary

I find automated CPDs easier only in the sense that they make
training new users easier. But they also can encourage not paying
sufficient attention to the drying instrument. I like the Polaron, in
particular it's large chamber. Let's see ... the only other advantage
to an automated system is if you have lots of samples to do in lots
of runs. Otherwise, I've never seen enough of an advantage to justify
the extra cost.

But! having said that, a CPD with autobleed is an excellent choice.
This is a good safety feature, and can help protect specimens.

Phil


There are safety considerations, that is one of the several reasons why
there is such a preference for the E3000 or E3100. BTW another good
thing about the Polaron design and larger chamber is the
less likelihood of turbulence, or putting it another way, you can do your
exchanges at a faster rate because you can use larger flow rates before
turbulence does set in.

Chuck (Garber)

We recently purchased a Tousimis
795 semi-automatic critical point
dryer for our multi-user facilty.
This unit replaces an old Denton
"bomb". We considered ease of use
for our customers to be an
important consideration. Our unit
has preset purge, bleed, vent and
heating features which make it
easier to use. We don't have to lug
a large beaker of hot water over to
the CPD to heat the chamber. The
flow rates are factory preset but
easily adjustable. Having these
features, I believe, allows us to
produce more consistent,
reproducible results.
The standard chamber size is rather
small but a chamber extension is
available and easy to install. We
have found that when trying to
critical point dry very large
specimens, the chamber size can be
a problem (most of our work is
Drosophila flies or mouse embryos).

Although very few people do their
own critical point drying here, our
goal is to make the facility as
user-friendly as possible, and this
seemed to be a step in that
direction.

Happy hunting.

Tom Januszewski
Senior Electron Microscopist
Molecular and Cellular Imaging
Facility

Rosemary Walsh, Manager
The Electron Microscope Facility for the Life Sciences,
A Shared Technology Facility, The Life Sciences Consortium
1 South FrearLab
Penn State University
University Park, PA 16802
(814) 865-0212
rw9-at-psu.edu
http://www.lsc.psu.edu/stf/em/home.html





From daemon Wed Oct 11 08:04:38 2000



From: Nagy Peter :      nagyp-at-chemres.hu
Date: Wed, 11 Oct 2000 08:02:17 -0500
Subject: Backscattered

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Collegues,

i'd like to purchase a backscattered detector for an old Hitachi SEM.
I am looking for vendors of such equipment, as Robinson detector, KE Si
detector and so on. Could you help me with some addresses?

Thanks,

Peter

--
Dr NAGY Peter
Chemical Research Center
Institute of Chemistry of the Hungarian Academy of Sciences
1025 Budapest Pusztaszeri ut 59-67.
Phone: 36-1-325-7548
Fax: 36-1-325-7509
E-mail: nagyp-at-chemres.hu





From daemon Wed Oct 11 08:29:02 2000



From: Chris Jeffree :      cjeffree-at-srv0.bio.ed.ac.uk
Date: Wed, 11 Oct 2000 14:23:13 +0000
Subject: RE: Stub replacement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Soji
We usually treat the standard aluminium LEO pin stubs as
disposable. Since we have a number of users each with a different
specimen type and mounting procedure it becomes difficult to
identify a single effective cleaning method, and staff time is more
costly than the stubs.

If you are a novice user you may not yet realise that LEO pin stubs
are obtainable at a fraction of LEO's price from most microscopy
supply houses.

http://www.kaker.com/mvd/list.html
is a list of microscopy vendors which you may find useful. Use this
to look up web sites of some of the following companies: Agar
Scientific, TAAB Laboratories, SPI, Electron Microscopy Science,
etc. etc.

However, cleaning stubs can be cost-effective if you routinely
produce many tens or hundreds of specimens in exactly the same
way. If you have to do this it pays to find adhesives and conductive
paints that redissolve readily in the same solvent, so things like
epoxies are out. Choose your solvents carefully, because some
like ethanol attack aluminium if it is immersed for long periods.

Another approach which avoids the need to clean stubs is to mount
up your specimens on thin squares or discs of metal (e.g. thin
aluminium sheet) which are then tacked to the stub using e.g.
graphite dag. After use the metal supports can be cracked off and
replaced with new ones. This way the stub can be re-used more
often before it needs to be properly cleaned up.
Good luck
Chris

} On Wednesday, October 11, 2000 3:09 AM, O. O. Ilori
} [SMTP:sojilori-at-oauife.edu.ng] wrote:
} }
} } Hello Guys
} } I am a novice user of a SEM (LEO 1450 to be precise) equipment which was
} } just installed in our central science lab.
} } Is there a way to clean stubs for reuse or are stubs disposable once used.
} } ( This may sound naive)
} } soji
} } Mr. O. O. ILORI
} } DEPARTMENT OF ELECTRONIC/ELECTRICAL ENGINEERING
} } OBAFEMI AWOLOWO UNIVERSITY,
} } ILE-IFE, OSUN STATE
} } NIGERIA.
} } email: sojilori-at-oauife.edu.ng
} }
} }
}
}


==========================================
Dr. Chris Jeffree
University of Edinburgh
BIOSEM - Biological Sciences Electron Microscope Facility
Institute of Cell and Molecular Biology
Daniel Rutherford Building
King's Buildings, Mayfield Road
EDINBURGH, EH9 3JH, Scotland, UK
Tel. #44 (0) 131 650 5345
FAX. #44 (0) 131 650 6563
Mobile 07710 585 401
email c.jeffree-at-ed.ac.uk
=========================================



From daemon Wed Oct 11 09:30:46 2000



From: Dohnalkova, Alice :      Alice.Dohnalkova-at-pnl.gov
Date: Wed, 11 Oct 2000 07:24:33 -0700
Subject: Bacterial quantitation in soil samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


(resubmitting message from Mon 9/10)


We are considering a purchase of a system for quantitative analysis of bacterial
and fungal biovolume in soil samples. Could anyone provide info on preferred
method (FITC labeling?) and equipment. As always, I appreciate your help,
Alice.

Alice Dohnalkova
Environmental Microbiology
Battelle, PNNL
MS P7-50
Richland, WA 99352
tel. (509) 372-0692
fax (509) 376-1321





From daemon Wed Oct 11 09:34:09 2000



From: White, Woody N. :      nwwhite-at-mcdermott.com
Date: Wed, 11 Oct 2000 09:28:26 -0500
Subject: RE: BSE Detector systems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ref: Diode detector type...
I have used GW Electronics. First on an ETEC SEM, and now on a Hitachi
S-3500. I started with a model (many years ago) which is now discontinued
and have upgraded to the current design. Both worked well for me. Check:
http://www.gwelectronics.com/

----------------------------------------
Woody White
McDermott Technology, Inc.
http://www.mtiresearch.com/

Me: http://home.att.net/~woody.white



From daemon Wed Oct 11 09:46:34 2000



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Wed, 11 Oct 2000 08:10:01 -0500
Subject: Re: bell jar treatment

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Peter
Try looking at this site.
http://www.kaker.com/mvd/list.html
It contains links to dozens of microscopy vendor sites
Chris

Date sent: Wed, 11 Oct 2000 08:02:17 -0500
To: Microscopy-at-sparc5.microscopy.com
} From: Nagy Peter {nagyp-at-chemres.hu}


The last time this subject came up, someone suggested wiping the inside of
the jar with Polaroid print coaters. They seem to provide a water soluble
layer that easily comes off. We used to have dozens of extras lying around
and now had a use for them. However, now that we have largely gone to
digital imaging we will have to watch our stock.

At 04:49 PM 10/10/2000 -0700, you wrote:

} Well we've got a new vacuum evaporator and it has been so long since I've
} had one I've forgotten how to treat the bell jar to reduce the effort it
} takes to keep it clean. However, I do recall that there were little
} tricks to cleaning and treating the glass and other surfaces. Anyone care
} to shed a little light my darkened memory of these procedures?

----------------------
Warren E. Straszheim
Materials Analysis and Research Lab
Iowa State University
23 Town Engineering
Ames IA, 50011-3232

Ph: 515-294-8187
FAX: 515-294-4563

E-Mail: wesaia-at-iastate.edu
Web: www.marl.iastate.edu

Scanning electron microscopy, x-ray analysis, and image analysis of materials
Computer applications and networking



From daemon Wed Oct 11 10:25:33 2000



From: Ladd Research :      ladres-at-worldnet.att.net
Date: Wed, 11 Oct 2000 11:22:39 -0400
Subject: Re: bell jar treatment

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We agree with John Nailon that if a user forgets to prepare the
evaporator prior to a run he should have to clean it.
Since we have to clean our evaporators several times a day because of
our substrate production, we do the following.

1. Place Victawet in a medium basket.
2. Evaporate the Victawet at 10(-5). This coats not only the bell jar,
but all the other surfaces under the bell jar as well.
3. Clean the system with windex (or water) and wipe with a lint free
cloth.
4. Re-coat with victawet prior to the next run.

John Arnott

Disclaimer: In additon to using, Ladd has been selling most of the above
mentioned products, including Victawet, Lint-free cloth, Substrates,
Evaporators and Baskets since 1955.
--

LADD RESEARCH
131 Dorset Lane
Williston, VT 05495 USA

web site http://www.laddresearch.com

TEL 1-800-451-3406 (US) or 1-802-878-6711 (anywhere)
FAX 1-802-878-8074
e-mail sales-at-laddresearch.com

Quality Since 1955


John Nailon wrote:
}
} ------------------------------------------------------------------------} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.}
} The easiest way to keep your bell jar clean is to wipe a thin film of
} detergent over the surface of the clean glass and pump down to dry.
} After each coating run simply rinse off the glass, the detergent comes
} away and takes the coating with it. Recoat with detergent and dry.
}
} Train ALL users in this technique, insist they clean the glass if they
} "FORGOT" to do it after their last run, and you will always have a
} clear, clean bell jar.
} Regards
} JVN
} Rick Harris wrote:
} }
} } ------------------------------------------------------------------------} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.} }
} } Well we've got a new vacuum evaporator and it has been so long since I've
} } had one I've forgotten how to treat the bell jar to reduce the effort it
} } takes to keep it clean. However, I do recall that there were little tricks
} } to cleaning and treating the glass and other surfaces. Anyone care to shed
} } a little light my darkened memory of these procedures?
} }
} } TIA
} }
} } Rick A. Harris, Director
} } Microscopy and Imaging Facility
} } Section of Molecular and Cellular Biology
} } 1241 Life Sciences Addition
} } University of California
} } Davis, CA
} } 530 752 2914
} } 530 754 7536 fax
} } http://katie.ucdavis.edu
} } raharris-at-ucdavis.edu
}
} --
} ****************************************************
} John V Nailon
} Operations Manager
} Centre for Microscopy and Microanalysis
} The University of Queensland
} St. Lucia Queensland 4072
} Phone: +61-7-3365-4214
} Fax: +61-7-3365-4422
} WWW: http://www.uq.edu.au/nanoworld/allstaff.html#Nailon
} ****************************************************



From daemon Wed Oct 11 10:38:06 2000



From: Joel McClintock :      jmcclin-at-pop.uky.edu
Date: Wed, 11 Oct 2000 23:36:54 -0400
Subject: Recoat Phosphor Screens

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Listers,

I need to have the phosphor screens recoated on an EM 400T we have on
campus. I have been told that Grant Scientific does a good job. I have not
been able to find a way to contact them. Can anyone help with a phone number
or info.? I would also welcome any other suggestions. Thanks.

Joel McClintock
EM Specialist
Univ. of Kentucky
Lexington, Ky
859-257-1242




From daemon Wed Oct 11 10:50:42 2000



From: Bernard Kestel :      kestel-at-anl.gov
Date: 11 Oct 00 10:48:02 -0600
Subject: Re: Bell Jar Cleaning:

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


To minimize the area which needs cleaning, I obtained a pyrex glass
cylinder large enough to fit vertically over my evaporating supports,
etc. The ends of it were coated with a vacuum rated epoxy to prevent
chipping.
Before using, it is coated with a commercial release agent made for this
"easy
cleaning" purpose. The bottom sits on a sheet of disposable aluminum foil
and
the top is also covered by aluminum foil. To facilitate pumping, a 1/4"
thick
scrap of ceramic placed under the bottom edge of the "containment
cylinder"
aids pumping at a reasonable rate and provides a path for one of the
power
leads for my evaporating basket, insulating the lead from a metal support

platform. After use, the foil can be scrapped and only a 6" diameter
cylinder
requires cleaning!

Bernie Kestel
Materials Science Division
Argonne National Laboratory
9700 South Cass Avenue
Argonne, Il., 60439

E-mail: {kestel-at-anl.gov}




From daemon Wed Oct 11 10:54:31 2000



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Wed, 11 Oct 2000 08:51:37 -0700
Subject: Re: bell jar treatment

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Dear Rick,
I used to use Victawet and it was the best, but it is not available, so I
use a bar of hand soap. After you clean the bell jar or, if it is new, after
you wet it, rub the dry bar of soap all over the inside and rub with a paper
towel to spread it evenly and make it transparent. Good luck.
At 04:49 PM 10/10/00 -0700, you wrote:
} Well we've got a new vacuum evaporator and it has been so long since I've
} had one I've forgotten how to treat the bell jar to reduce the effort it
} takes to keep it clean. However, I do recall that there were little tricks
} to cleaning and treating the glass and other surfaces. Anyone care to shed
} a little light my darkened memory of these procedures?
}
} TIA
}
} Rick A. Harris, Director
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca




From daemon Wed Oct 11 11:03:42 2000



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Wed, 11 Oct 2000 08:56:48 -0700
Subject: Re: SEM: Stub replacement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Mr. Olori,
The stubs used for SEM are reused for years, but the grids for a TEM are
disposed of. I clean my SEM stubs by rubbing on a paper towel wet with
either lab alcohol (ethanol) or acetone, depending what was on them. If I am
using the carbon double-side sticky tabs, I soak the stubs in acetone for an
hour, then rub on a paper towel to remove the tab. Congratulations on your
new SEM.
At 06:09 PM 10/10/00 +0100, you wrote:
}
} Hello Guys
} I am a novice user of a SEM (LEO 1450 to be precise) equipment which was
} just installed in our central science lab.
} Is there a way to clean stubs for reuse or are stubs disposable once used.
} ( This may sound naive)
} soji
} Mr. O. O. ILORI
} DEPARTMENT OF ELECTRONIC/ELECTRICAL ENGINEERING
} OBAFEMI AWOLOWO UNIVERSITY,
} ILE-IFE, OSUN STATE
} NIGERIA.
} email: sojilori-at-oauife.edu.ng

Good luck,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca




From daemon Wed Oct 11 11:19:14 2000



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Wed, 11 Oct 2000 12:19:51 -0500
Subject: BSE detector vendors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Peter Nagy wrote:
==========================================================
i'd like to purchase a backscattered detector for an old Hitachi SEM. I am
looking for vendors of such equipment, as Robinson detector, KE Si detector
and so on. Could you help me with some addresses?
===========================================================
Try
Microscopy Vendor's Data Base
http://207.137.96.185/mvd/products/back.html

They have them all, I think.



Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.spi.cc
########################
============================================



From daemon Wed Oct 11 11:36:05 2000



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Wed, 11 Oct 2000 11:33:08 -0500
Subject: Re: digital imaging info needed

Contents Retrieved from Microscopy Listserver Archives
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I suppose that John Russ and others have stated the matter concisely and
eloquently in their books and articles on the subject, but the concepts are
not that difficult that I will take a stab at it.

A digital image is nothing more than a two-dimensional array of numbers
where the numbers represent some characteristic of the image. Various image
sources vary in the way that the arrays are assembled and what the numbers
represent.


In an scanning electron microscope or a scanning probe microscope (which I
suppose you could say that an SEM is), a single detector element is used to
provide the signal at all points in the image. The X and Y coordinates of
the scan are used to determine the array or pixel index, and the signal is
digitized at each point.

Many systems utilize "active" control where the beam is directed to each
coordinate in succession under computer control. A digital grid is
predetermined within the computer and the coordinates are fed out through
appropriate hardware to convert the digital coordinates to the necessary
analog voltage to scan the probe to the desired point. A microscope signal
is measured at that point and digitized for storage in the image array. The
scan speed and resolution are under direct control of a computer.

Other systems are termed "passive" since the existing analog microscope
scan system is used to control the probe scan as normal. The digitizing
system monitors the x and y coordinates and records the signal level when
the beam is in positions corresponding to the pixels in the image array.

There are a variety of signals available from electron microscopes.
Detectors for secondary electrons and backscattered electrons operate as
analog devices. (One might argue that since they are counting a finite
number of electrons, then they could be setup as digital devices. However,
the count rate is high enough that digital detectors are probably not
feasible, and even if they were there remains a question of when to start
counting.) A variety of detectors and devices are used (including PMTs -
photo multiplier tubes) to measure the signal, but they are primarily, if
not all, analog devices. An analog to digital convertor is used to convert
the signal to digital for the image array. Some scopes may digitize the
signal early on so that no analog signal is readily available, but it still
exists somewhere inside the instrument.

X-ray signals in an electron microscope are probably treated as digital
from the outset. Since the x-ray photons are detected as pulses from the
x-ray detector, they are treated as discrete events and counted. Since
counting is involved, x-ray signals are only used with active control
systems (to the best of my knowledge). A counter is reset each time the
probe is moved to a new location.


There is a whole other class of source for digital images. In this class
there is a separate detector for each element of the array. The entire
image is present at once as opposed to being built up by a scanned probe.
The detectors operate in parallel as they build up and digitize the signal
simultaneously. At some point the signal is read and the detectors are
reset. CCD cameras are the most common device in this category. They find
application in light microscopes and transmission electron microscopes as
well as in consumer electronics such as video cameras and still cameras.

In these systems, the images are digital from the start. The readout is in
terms of an electron count. It usually gets scaled to another digital range
(0-255, 0-4095, etc) so that the original digital count may no longer be
significant.

The size of the image array is determined by the size of the CCD array.
Smaller image sizes can be obtained by grouping the signals from adjacent
elements in the CCD array. Larger images are sometimes manufactured by
interpolating the signals of neighboring elements, but that is not
introducing new information.


I hope this helps. Feel free to ask for further clarification.

Warren S.

At 06:40 PM 10/10/2000 -0400, you wrote:
} Dear listserv:
}
} Dee Breger was kind enough to forward this message for me. I'm writing a book
} on digital photography, to be published by Harry N. Abrams next year. The book
} will include a section on applied digital imaging, and I am particularly
} interested in scientific imaging. I am hoping that some of you folks might
} be able
} to provide some information.
}
} For the purposes of the book, I am defining digital imaging as that which
} describes a scene using discrete digital information in a raster grid, or
} bitmap. So, I am particularly interested in scientific imaging applications
} which gather information in this way.
}
} I would be interested to hear if there are other scientific imaging systems
} (DEE: besides SEMs) which collect information in this manner. For instance,
} it sounds as though atomic force microscopes work in this way: as they
} drag a stylus across a surface, the position is recorded (x and y), along
} with the height (z). This
} is later used to re-create the image in a raster grid (the fact that the AFM
} does not use light makes it another interesting foil to 'traditional' digital
} photography). How then, are colors in the final output determined from the
} data collected? I would also, of course, love to see any images you might have
} to show.
}
} Thanks in advance,
} Jonathan Lipkin (jlipkin-at-ramapo.edu)
}
} DEE: I'll forward to Jonathan any replies that are publically posted to the
} listserver. He also needs other detailed technical information on digital
} image formation which can be far more comprehensively clarified and
} amplified by some of the experts among my fellow listers than by me, though
} I've already given him the broad picture for analog scopes like my dear old
} Cambridge 250. His SEM impressions, which I'll pass along verbatim,
} include (1) "SEMs collect information, used to fill a raster grid, through
} the use of a PMT", (2) the signal is sent directly to the crt in analog
} form -I always had thought that the pmt sent out digital info, but it
} sounds like it just sends out analog data (a waveform? though it couldn't
} be a waveform if the raster grid is discrete in two dimensions)" and (3)
} "as the secondary electrons come off the sample, they hit a detector which
} generates a digital number (redundant) which is then placed in a pixel of a
} bitmap."
}
} Many thanks to any who can help!

----------------------
Warren E. Straszheim
Materials Analysis and Research Lab
Iowa State University
23 Town Engineering
Ames IA, 50011-3232

Ph: 515-294-8187
FAX: 515-294-4563

E-Mail: wesaia-at-iastate.edu
Web: www.marl.iastate.edu

Scanning electron microscopy, x-ray analysis, and image analysis of materials
Computer applications and networking



From daemon Wed Oct 11 12:11:19 2000



From: A. Greene :      ablue-at-io.com
Date: Wednesday, October 11, 2000 11:45 AM
Subject: Recoat Phosphor Screens

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Joel,

Grant does very fine work. As a mater of fact they are the source used by a
few OEMs.
Their address is 1385 Rock Island Road
Gilbert South Carolina 29054
Phone: 803/892-2841

Regards,

Alex Greene
SCIENTIFIC INSTRUMENTATION SERVICES, INC.
PMB-499, 1807 West Slaughter Lane, Number 200
Austin, Texas 78748-6200
Phone: 512/282-5507 Fax: 512/280-0702

QUALITY ELECTRON MICROSCOPE REPAIR
-----Original Message-----
} From: Joel McClintock {jmcclin-at-pop.uky.edu}
To: Microscopy-at-sparc5.microscopy.com {Microscopy-at-sparc5.microscopy.com}




From daemon Wed Oct 11 12:16:42 2000



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Wed, 11 Oct 2000 18:08:26 +0100 (GMT Daylight Time)
Subject: Re: RE: Stub replacement

Contents Retrieved from Microscopy Listserver Archives
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We slice off the specimen with a razor blade and use the
blade to scrape the stub fairly clean. As we use colloidal
graphite, which is conductive, I do not mind some residual
glue as it will get covered by glue from the next use.
This glue can be removed, if required, by sonicating in
butyl acetate.

Dave


On Wed, 11 Oct 2000 21:09:07 +1000 Jim at ProSciTech
{jim-at-proscitech.com} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} It all depends: how busy you are, how expensive staff time in your country is
} and how costly the place is you buy your stubs from. Obviously some specimens
} should be stored in a desiccator for an eternity and other can be discarded
} immediately.
} There is nothing magical about cleaning stubs. Solvents are messy. Best to
} place on some newspaper a very coarse file or a sheet of coarse (50 grit)
} sandpaper and then rub the old stub over that file of sandpaper until specimen
} and glue are removed.
}
} Disclaimer: ProSciTech sells stubs, but we are happy if people recycle and this
} leaves some money for useful purchases.
} Cheers
} Jim Darley
} ProSciTech Microscopy PLUS
} PO Box 111, Thuringowa QLD 4817 Australia
} Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
} Great microscopy catalogue, 500 Links, MSDS, User Notes
} ABN: 99 724 136 560 www.proscitech.com
}
} On Wednesday, October 11, 2000 3:09 AM, O. O. Ilori
} [SMTP:sojilori-at-oauife.edu.ng] wrote:
} }
} } Hello Guys
} } I am a novice user of a SEM (LEO 1450 to be precise) equipment which was
} } just installed in our central science lab.
} } Is there a way to clean stubs for reuse or are stubs disposable once used.
} } ( This may sound naive)
} } soji
} } Mr. O. O. ILORI
} } DEPARTMENT OF ELECTRONIC/ELECTRICAL ENGINEERING
} } OBAFEMI AWOLOWO UNIVERSITY,
} } ILE-IFE, OSUN STATE
} } NIGERIA.
} } email: sojilori-at-oauife.edu.ng
} }
} }
}
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"




From daemon Wed Oct 11 12:30:47 2000



From: William F. Tivol :      wft03-at-health.state.ny.us
Date: Wed, 11 Oct 2000 13:26:36 -0400
Subject: Re: Recoat Phosphor Screens

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html






I need to have the phosphor screens recoated on an EM 400T we have on
campus. I have been told that Grant Scientific does a good job. I have not
been able to find a way to contact them. Can anyone help with a phone number
or info.? I would also welcome any other suggestions. Thanks.
Dear Joel,
(803)892-2841. I just tried it, and it works. I also think that Grant did
a good job for us.
Yours,

Bill Tivol
Wadsworth Center
Albany NY
(518) 473-7399 WFT02-at-health.state.ny.us





From daemon Wed Oct 11 12:32:51 2000



From: Rosanne Healy :      rhealy-at-iastate.edu
Date: Wed, 11 Oct 2000 12:29:39 -0500
Subject: infiltration of leaf/jet freeze fix

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi List,
This is a question/situation a colleague of mine wanted to post-her
email for replies not on the list is rhealy-at-iastate.edu.
Of all people out there, I know you all can help!
Thanks so much!
Tracey Pepper

We are working with Cochliobolus inoculated maize leaf tissue using jet-propane
fixation, and freeze substitution using acetone (for immuno resins we switch to
ultra pure ETOH prior to infiltration), and have attempted using several
immuno-type resins such as: Lowicryl HM20 (with poly. at -20 under UV), LR
White with accel. at 4deg., and Quetol for general morphology. Our ultimate
goal is to visualize extra-cellular matrix of fungus within leaf tissues using
immunocytochem. So far, fixation is great (general morph.) However, the
immuno resins are not infiltrating adequately (under -30 deg.for Lowicryl and
4 deg. for LR White), lots of separation, and shattering. The longest we have
infiltrated has been 1-2 hr. each step for LR White. The Lowicryl had an
overnight in pure followed by 1/2 day for each of five total changes.
Any and all suggestions would be greatly appreciated!
Regards,
Rosanne Healy



Tracey Pepper
Supervisor
Bessey Microscopy Facility
Iowa State University
ph: 515-294-3872
fax: 515.294.1337




From daemon Wed Oct 11 12:43:05 2000



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Wed, 11 Oct 2000 13:39:52 -0500
Subject: Coater for use with FESEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Ben Craft wrote:
=============================================================
I'm getting ready to purchase a new sputter coater for our FESEM. I'd
like to hear how lucky others were with using a "regular" sputter coater
for high resolution SEM work. Currently, there is a noise problem with our
SEM, so the resolution is about 3-4nm. I've successfully imaged 5nm ZrO2
close-packed particles, and 4nm pores in glass using a thin coating of Au/Pd
applied with an old Polaron. One thing I'm worried about is = once the noise
is fixed and I can resolve the published 1.5nm resolution will I be able to
resolve the grains in the coating?
=============================================================
One system to consider is the Osmium Plasma Coater OPC-60 made by Nippon
Laser and Electronics, Nagoya, Japan. It is distributed by SPI Supplies.
You can find extensive information and examples on URL
http://www.2spi.com/catalog/osmi-coat.html

The end result is an amorphous coating so there is no grain size with which
one has to contend. The source of the osmium is an ampoule of osmium
tetroxide. This is not just a simple matter of sputtering from an osmium
metal cathode. And since the coating is an inert precious group metal, its
shelf life is quite long, if not infinite. Safety concerns are address on
the webpage mentioned above.

Contact me off-line should you want us to do a demo coating for you.

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.spi.cc
########################
============================================



From daemon Wed Oct 11 13:34:00 2000



From: Pauline C. Yu :      splene-at-pw.usda.gov
Date: Wed, 11 Oct 2000 11:27:52 -0700 (PDT)
Subject: questions for Pelco CPD2 users

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi listers

We just bought a Pelco CPD2 with the hopes of replacing our old Polaron
E3000, and so far, we're rather unimpressed just trying to get it through
an initial setup.
We were wondering if any other users could tell us how many runs they get
out of an average CO2 cylinder? The cooling process with the CO2 seems
somewhat inefficient at this point and we're using up a lot just trying to
cool the chamber. Also any recommendations on the soak vs. the rinse
methods of infiltrating the samples would be appreciated.
I can only assume the operations get a little easier with practise, but
right now we're wondering whether it's worth the investment in the long
run. The Polaron has been very reliable and has served us well, but we're
worried that its age is compromising its safety.

Thanks in advance,

Pauline Yu
splene-at-pw.usda.gov
Microscopist Technician
USDA-ARS-WRRC




From daemon Wed Oct 11 14:44:04 2000



From: John J. Bozzola :      bozzola-at-siu.edu
Date: Wed, 11 Oct 2000 14:38:56 -0500
Subject: Poster-sized Printers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Our microscopy facility recently merged with the scientific
photography and graphics production unit on campus.
We need to quickly evaluate the potential value of a large format
inkjet printer for producing posters for scientific meetings.

We welcome comments, suggestions and especially personal experiences
using inkjet technology for poster production. Is it worth the
expense, do researchers use it, etc... We are looking at the Epson
9000 printer in combination with the Fujifilm digital 9000 Photo
Graphix RIP. Good idea? How about software for poster production?

Also, vendors are welcome to contact me and to submit quotations. We
need all of this information by Friday (Oct 13). Sorry for the rush
but I just now got the word that funds may be available. Whew....

Many thanks....


John B.

--
####################################################################
John J. Bozzola, Ph.D., Director
IMAGE (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
####################################################################



From daemon Wed Oct 11 15:29:26 2000



From: Ladd Research :      ladres-at-worldnet.att.net
Date: Wed, 11 Oct 2000 16:26:14 -0400
Subject: Re: bell jar treatment

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Mary Mager,

Victawet is a stock item for Ladd. We sell and use it on a daily basis.
Please let me know off-line if you want a quote.

Thanks,

JD Arnott


--

LADD RESEARCH
131 Dorset Lane
Williston, VT 05495 USA

web site http://www.laddresearch.com

TEL 1-800-451-3406 (US) or 1-802-878-6711 (anywhere)
FAX 1-802-878-8074
e-mail sales-at-laddresearch.com

Quality Since 1955
Mary Mager wrote:
}
} ------------------------------------------------------------------------} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.}
} Dear Rick,
} I used to use Victawet and it was the best, but it is not available, so I
} use a bar of hand soap. After you clean the bell jar or, if it is new, after
} you wet it, rub the dry bar of soap all over the inside and rub with a paper
} towel to spread it evenly and make it transparent. Good luck.
} At 04:49 PM 10/10/00 -0700, you wrote:
} } Well we've got a new vacuum evaporator and it has been so long since I've
} } had one I've forgotten how to treat the bell jar to reduce the effort it
} } takes to keep it clean. However, I do recall that there were little tricks
} } to cleaning and treating the glass and other surfaces. Anyone care to shed
} } a little light my darkened memory of these procedures?
} }
} } TIA
} }
} } Rick A. Harris, Director
} Regards,
} Mary
}
} Mary Mager
} Electron Microscopist
} Metals and Materials Engineering
} University of British Columbia
} 6350 Stores Road
} Vancouver, B.C. V6T 1Z4
} CANADA
} tel: 604-822-5648
} e-mail: mager-at-interchg.ubc.ca



From daemon Wed Oct 11 15:55:04 2000



From: jim quinn :      jquinn-at-doL1.eng.sunysb.edu
Date: Wed, 11 Oct 2000 16:47:28 -0400
Subject: Re: Recoat Phosphor Screens

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


GRANT SCIENTIFIC CORP
1385 ROCK ISLAND ROAD
GILBERT , SC 29054
803-892-2841 PHONE
803-892-2441 FAX

} Listers,
}
} I need to have the phosphor screens recoated on an EM 400T we have on
} campus. I have been told that Grant Scientific does a good job. I have not
} been able to find a way to contact them. Can anyone help with a phone number
} or info.? I would also welcome any other suggestions. Thanks.
}
} Joel McClintock
} EM Specialist
} Univ. of Kentucky
} Lexington, Ky
} 859-257-1242



From daemon Wed Oct 11 17:44:46 2000



From: Brian W Robertson :      brobertson-at-unl.edu
Date: Wed, 11 Oct 2000 17:38:54 -0500
Subject: EM400T available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We are moving out our Philips EM400T TEM. It has suffered from vacuum
logic and lens logic problems -- problems that currently mean that it is
unusable. It has a STEM unit, a Quantum EDX detector, Kevex 7000 system,
and a variety of holders, including tilt-rotate. Anyone interested in some
or all of this equipment should get in touch ASAP, please.
Thanks,
Brian Robertson


***********************************************************
Assoc. Prof. Brian W. Robertson
Department of Mechanical Engineering
and Center for Materials Research and Analysis
University of Nebraska-Lincoln, N124 WSEC,
17th & Vine Sts., Lincoln, NE 68588-0656, USA
** Office 402 472 8308; FAX 472 1465; Labs 472 8762 and 472 5498 **



From daemon Wed Oct 11 20:04:17 2000



From: Vaughn Fierke :      Vfierke-at-snblusa.com
Date: Wed, 11 Oct 2000 19:56:06 -0500
Subject: Ultracut

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have a Reichert Jung Ultracut S/N382797, Type701701, acquired from Upjohn
Japan. The unit has no stereoscope or power unit (lost in packaging or
shipping). Any person interested in the unit to use or for parts, please
don't hesitate to contact me.





From daemon Wed Oct 11 22:54:23 2000



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Wed, 11 Oct 2000 23:54:51 -0500
Subject: Polaron CPD question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Pauline Yu wrote:
===========================================================
........................ The Polaron has been very reliable and has
served us well, but we're
worried that its age is compromising its safety.
==========================================================
Unless the unit has been dropped or otherwise abused, it is difficult for me
to see how its "age" could have anything to do with its safety. But if this
was your concern, there is a process, and we could help you arrange it, by
which the chamber is returned to the UK, and retested in the same laboratory
where it was tested originally, and essentially recertified. The testing is
done at a pressure that is far beyond where a rupture disc would blow, so
you could then use it with confidence for many years to come. The cost of
the service is far less than the cost of a new system.

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.spi.cc
########################
============================================



From daemon Thu Oct 12 08:06:18 2000



From: tellis2-at-hallmark.com
Date: Thu, 12 Oct 2000 08:00:40 -0500
Subject: stub cleaning

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I experimented with several different cleaning/mounting procedures and the
one that is the most time effective for me is the following:
I apply a conductive silicone release agent to my stubs before I
apply carbon double stick tape to them, then I use a conductive Al or Cu
tape ( I cut it in half length-wise so it doesn't take up so much space )
to insure conductivity, works for my samples which are mostly dry powders,
industrial hygiene dusts, inks and papers.
Then when I want to clean the stubs I just lift the tape off with a
knife ( not to sharp ) and rinse and rub any residue off with paper towels
using a 50/50 % toluene / acetone mix, then ethanol in a ultrasonic
cleaner if I need them really clean.

Terry Ellis
Hallmark Cards Inc.
tellis2-at-hallmark.com
USA.




From daemon Thu Oct 12 08:06:18 2000



From: Zhongwen Yao :      zong-wen.yao-at-psi.ch
Date: Thu, 12 Oct 2000 08:02:18 -0500
Subject: copper eclectrical polishing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Listers, I meet some problem how to electrical-polish 3mm pure copper
disk. Would you plese tell me the good solution and the good condition
of electrical-polishing copper on tenupol-5 machine.
Thanks

Mr.Zhongwen Yao
EPFL- CRPP
Villingen, PSI
Switzerland





From daemon Thu Oct 12 08:06:18 2000



From: Gareth Robinson :      Gareth.Robinson-at-emitech.co.uk
Date: Thu, 12 Oct 2000 13:51:49 +0100
Subject: RE: Coater for use with FESEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Ben,

You may also want to consider the option of Chromium coating which will
give grain siz of less than 0.5NM Chromium grain size with thin film
deposition typically 5NM.

We manufacture and sell the K575 Sputter Coater unit. It has the
advantage of automated cycle coating Turbomolecular drag pumping for
High Vaccum.

Please take a look at our web site for a detailed technical brief and
the instrument specification.

HTTP://www.emitech.co.uk

Kind regards

Gareth
} -----Original Message-----
} From: Garber, Charles A. [SMTP:cgarber-at-2spi.com]
} Sent: Wednesday, October 11, 2000 7:40 PM
} To: MICROSCOPY BB
} Subject: Coater for use with FESEM
}
} ----------------------------------------------------------------------
} --
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------
} -.
}
}
} -- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --
}
} Ben Craft wrote:
} =============================================================
} I'm getting ready to purchase a new sputter coater for our FESEM. I'd
} like to hear how lucky others were with using a "regular" sputter
} coater
} for high resolution SEM work. Currently, there is a noise problem
} with our
} SEM, so the resolution is about 3-4nm. I've successfully imaged 5nm
} ZrO2
} close-packed particles, and 4nm pores in glass using a thin coating of
} Au/Pd
} applied with an old Polaron. One thing I'm worried about is = once the
} noise
} is fixed and I can resolve the published 1.5nm resolution will I be
} able to
} resolve the grains in the coating?
} =============================================================
} One system to consider is the Osmium Plasma Coater OPC-60 made by
} Nippon
} Laser and Electronics, Nagoya, Japan. It is distributed by SPI
} Supplies.
} You can find extensive information and examples on URL
} http://www.2spi.com/catalog/osmi-coat.html
}
} The end result is an amorphous coating so there is no grain size with
} which
} one has to contend. The source of the osmium is an ampoule of osmium
} tetroxide. This is not just a simple matter of sputtering from an
} osmium
} metal cathode. And since the coating is an inert precious group
} metal, its
} shelf life is quite long, if not infinite. Safety concerns are
} address on
} the webpage mentioned above.
}
} Contact me off-line should you want us to do a demo coating for you.
}
} Chuck
}
} ============================================
}
} Charles A. Garber, Ph. D. Ph: 1-610-436-5400
} President 1-800-2424-SPI
} SPI SUPPLIES FAX: 1-610-436-5755
} PO BOX 656 e-mail:cgarber-at-2spi.com
} West Chester, PA 19381-0656 USA
} Cust.Service: spi2spi-at-2spi.com
}
} Look for us!
} ########################
} WWW: http://www.spi.cc
} ########################
} ============================================



From daemon Thu Oct 12 08:56:21 2000



From: Gunnar Glasser :      glasser-at-mpip-mainz.mpg.de
Date: Thu, 12 Oct 2000 15:56:51 +0200
Subject: Inspection of "very thin" Au-films[PVD] by FESEM?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

I am looking for help concerning the following problem: Someone in our
institute is trying to deposit a 10nm Au-film on a Ge single crystal by
thermal evaporation from a tungsten boat (...rate: 0.5A/s).
The main point to me is, if FESEM is an appropriate method to determine if
we have really a Au-film or only gold islands ...

I do appreciate any comments concering both points (FESEM-tricks,
because 99% of our samples are uncoated insulators) and the depostition
technique/parameters for very thin gold films ...

ThanX very much in advance!

Dipl.Ing.(FH) G.Glasser
Elektronenmikroskopie
Max Planck Institut fuer Polymerforschung
Ackermannweg 10
55128 Mainz, GERMANY
phone: ++49 +6131 379195
fax : ++49 +6131 379100
web: http://www.mpip-mainz.mpg.de/~glasser

Disclaimer: The above statement is mine alone and does not implicitly represent the position of the MPI-P or the MPG!



From daemon Thu Oct 12 08:57:06 2000



From: Gunnar Glasser :      glasser-at-mpip-mainz.mpg.de
Date: Thu, 12 Oct 2000 15:58:11 +0200
Subject: Inspection of "very thin" Au-films[PVD] by FESEM?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

I am looking for help concerning the following problem: Someone in our
institute is trying to deposit a 10nm Au-film on a Ge single crystal by
thermal evaporation from a tungsten boat (...rate: 0.5A/s).
The main point to me is, if FESEM is an appropriate method to determine if
we
have really a Au-film or only gold islands ...

I do appreciate any comments concering both points (FESEM-tricks,
because 99% of our samples are uncoated insulators) and the depostition
technique/parameters for very thin gold films ...

ThanX very much in advance!



Dipl.Ing.(FH) G.Glasser
Elektronenmikroskopie
Max Planck Institut fuer Polymerforschung
Ackermannweg 10
55128 Mainz, GERMANY
phone: ++49 +6131 379195
fax : ++49 +6131 379100
web: http://www.mpip-mainz.mpg.de/~glasser

Disclaimer: The above statement is mine alone and does not implicitly represent the position of the MPI-P or the MPG!



From daemon Thu Oct 12 09:02:26 2000



From: Tobias Baskin :      BaskinT-at-missouri.edu
Date: Thu, 12 Oct 2000 09:00:05 -0500
Subject: RE: Coater for use with FESEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greetings,
As seen below, Gareth Robinson mentions chromium as an option
for fine coats to use in high res SEM.

A word of caution: Chromium is tricky to work with. You
cannot use chromium sputter coating the same simply way you are used
to sputtering with, say, gold or platinum. You have to keep the
atmosphere in the coater squeaky clean, and even cleaner, and you
must absolutely minimize the exposure of your coated sample to oxygen.

I expect that chromium coats can be superb, but you cannot
expect to simply put a chromium target in your sputter coater and
have decent results. We found this out the hard way.

As ever,
Tobias

}
}
}
} Ben,
}
} You may also want to consider the option of Chromium coating which will
} give grain siz of less than 0.5NM Chromium grain size with thin film
} deposition typically 5NM.
}
} We manufacture and sell the K575 Sputter Coater unit. It has the
} advantage of automated cycle coating Turbomolecular drag pumping for
} High Vaccum.
}
} Please take a look at our web site for a detailed technical brief and
} the instrument specification.
}
} HTTP://www.emitech.co.uk
}
} Kind regards
}
} Gareth Gareth.Robinson-at-emitech.co.uk

--
_ ____ __ ____ Tobias I. Baskin
/ \ / / \ / \ \ 109 Tucker Hall
/ / / / \ \ \ Biological Sciences
/_ / __ /__ \ \ \__ University of Missouri
/ / / \ \ \ Columbia, MO USA
/ / / \ \ \ 65211-7400
/ / ___ / \ \__/ \ ____ voice: 573-882-0173
fax: 573-882-0123



From daemon Thu Oct 12 09:15:48 2000



From: Lucille A. Giannuzzi :      lag-at-mail.ucf.edu
Date: Thu, 12 Oct 2000 10:12:38 -0400
Subject: Re: EM400T available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 5:38 PM -0500 10/11/00, Brian W Robertson wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Brian,

I am interested in the holders.

Regards,
Lucille Giannuzzi

*******************************************************************
Lucille A. Giannuzzi, Ph.D.

Associate Professor, Mechanical, Materials, and Aerospace Engineering
Director, UCF/Cirent Materials Characterization Facility
University of Central Florida
12443 Research Parkway, Suite 305
Orlando, FL 32826 email lag-at-mail.ucf.edu phone (407) 275-4354,5,6
fax (407) 275-4321
--------------------------------------------------------------------
"Good judgement comes from experience.

Experience comes from making bad judgement."

Mark Twain
********************************************************************





From daemon Thu Oct 12 10:52:23 2000



From: Steve Barlow :      sbarlow-at-sunstroke.sdsu.edu
Date: Thu, 12 Oct 2000 08:50:11 -0700
Subject: light microscope lenses

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Netters

In upgrading our light microscopes, we are buying some new lenses for our
confocal scope and a brightfield scope. The salesforce is regaling us with
tales of improved resolution, clarity, brightness etc that comes with
spending double or triple the price for a standard oil immersion or dry
lens. Could someone please clarify, either on-line or off, what the
advantages and disadvantages of each of the following ( my texts on light
microscopy don't deal with them). Are they worth the extra dollars?

1) a water immersion lens for looking at cells suspended in buffer and
covered with a coverslip (water is used rather than oil between coverslip
and lens) These lenses typically have a lower numerical aperture than an
oil immersion lens.

2) a water dipping lens, used to examine cells in culture directly, without
a coverslip. I would expect the absence of refractive indice changes
(media/coverslip/air or oil) would explain their improved brightness.

Thanks in advance for your input

Steve Barlow
SDSU EM Facility





From daemon Thu Oct 12 11:18:57 2000



From: David Henriks :      Henriks-at-CompuServe.COM
Date: Thu, 12 Oct 2000 12:09:00 -0400
Subject: "coater" for High Resolution SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ben:

As you are so close by to our San Clemente, CA lab, I would suggest that
you come by and bring some samples to try on our IBS/e Ion Beam Sputter
Deposition and Etching System.

The IBS/e, a thin film deposition and etching system, is designed to
improve high resolution electron microscopy imaging by depositing
ultra-thin, fine grain metal and carbon films on specimens. Even low
voltage imaging is improved.

We can deposit many different metals and carbon or show you examples of
contrast enhancement on various types of specimens from our library of
micrographs. I will send to you by separate mail some AFM images comparing
evaporated coatings, sputtered coatings and ion beam deposited films.

DISCLAIMER: South Bay Technology produces equipment and supplies as
described above and, therefore, has a vested interest in promoting their
use.

Best regards-

David
Writing at 8:27:56 AM on 10/11/2000

***************************************************************************
************************

David Henriks
Vice President TEL: 800-728-2233
(toll free in the USA)
South Bay Technology, Inc. +1-949-492-2600
1120 Via Callejon FAX: +1-949-492-1499
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

***************************************************************************
************************

} } } } } Please visit us at http://www.southbaytech.com { { { { {

Manufacturers of precision sample preparation equipment and supplies for
metallography, crystallography and electron microscopy.

Message text written by Ben Craft
}
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


I'm getting ready to purchase a new sputter coater for our FESEM. I'd
like to hear how lucky others were with using a "regular" sputter coater
for high resolution SEM work. Currently, there is a noise problem with
our SEM, so the resolution is about 3-4nm. I've successfully imaged 5nm
ZrO2 close-packed particles, and 4nm pores in glass using a thin coating
of Au/Pd applied with an old Polaron. One thing I'm worried about is =
once the noise is fixed and I can resolve the published 1.5nm resolution
will I be able to resolve the grains in the coating?

Thanks,
Ben {



From daemon Thu Oct 12 11:45:45 2000



From: Becky Holdford :      r-holdford-at-ti.com
Date: Thu, 12 Oct 2000 11:56:53 -0500
Subject: 200 Fall Meeting of the Texas Society for Microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The 2000 Fall Meeting of the Texas Society for Microscopy will be held

on October 26, 27, and 28 at the DoubleTree Hotel in Dallas, TX.
The hotel is located in Campbell Centre at 8350 N. Central
Expressway, Dallas, TX 75206. The phone number for the hotel is
800-222-TREE.
A special workshop will be held on Thursday, Oct. 26, entitled "The
SEM Today". It will cover the latest information concerning basic
SEM,
LVSEM, ESEM and detectors. Dr. David Joy, University of Tennessee,
will be
speaking on Low Voltage SEM and Variable Pressure SEM.
YOU MUST BE REGISTERED FOR THE THURSDAY
SEMINAR SERIES. It is free for members and new member applicants.
If you plan to attend and have not registered, call or email Pam Neill
at
817-568-6497 or pamela.neill-at-alconlabs.com

TSM PROGRAM
SUMMARY OF DAILY ACTIVITIES

Thursday October 26, 2000
8:30-9:00 Registration at TI
9:00-5:00 Workshop coordinated by Kevin Cronyn, Hitachi and hosted by
TI
12:30-7:00 Exhibitor setup and Poster setup DoubleTree
5:30-7:00 TSM Executive council Meeting, DoubleTree
7:00-9:00 Evening Social, DoubleTree
9:30-11:00 TSM Hospitality room DoubleTree

Friday October 27, 2000
8:00-8:30 Continental Breakfast in vendor's room
8:00-11:00 Registration
8:30-8:45 Introduction and announcements.
8:45-10:00 Platform presentations
10:00-10:15 Break in exhibitors’ room.
10:15-11:30 Guest speaker Russ Pinazotto "Electron Microscopy of
Semiconductor Nanoparticles"
11:45-1:45 Lunch and business meeting with vendors at DoubleTree Hotel
Campbell Center
1:45-3:00 Guest speaker Dr. Charles Mims “Use of High Pressure
Freezing for Biological TEM".
He will also include some information on plunge freezing.
3:00-4:30 Break in exhibitors’ room. Poster authors should be at
their posts
5:30-7:00 Social in the hospitality suite. Dinner on your own. There
will be a trip planned to
the West End via DART for dinner and entertainment.

Saturday October 28, 2000
8:00-9:00 Registration
8:00-9:00 Continental Breakfast
8:00-9:15 Poster session continued
9:30-10:45 Platform presentations
10:45 adjourn


For more information and/or to register, please contact
Pamela Neill, Program Chair TSM
Alcon Research LTD
6201 South Freeway
Forth Worth, TX 76134-2099
pamela.neill-at-alconlabs.com
817-568-6497
--
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Becky Holdford (r-holdford-at-ti.com)
972-598-1291 (pager)
KFAB Physical Analysis Lab--SEM/FIB/FA
Texas Instruments, Inc.
Dallas, TX
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~





From daemon Thu Oct 12 11:49:24 2000



From: Malis, Tom :      malis-at-nrcan.gc.ca
Date: Thu, 12 Oct 2000 12:46:52 -0400
Subject: RE: copper eclectrical polishing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Here are a few Cu electropolishing solutions:

a) 33% nitric acid, 67% methanol cooled to -25C and at 1.5 V

b) 45% phosphoric acid, 55% water at { 10C (no voltage given)

c) 10% orthophosphoric, 90% water at 0C and at 8 V.

I had success with the first solution in a Tenupol more years ago than I
care to contemplate!

Tom Malis
Group Leader - Characterization
Materials Technology Laboratory
Natural Resources Canada (Govt. of Canada)
568 Booth St., Ottawa, Canada
ph. 613-992-2310
FAX 613-992-8735
email: malis-at-nrcan.gc.ca


----------
From: Zhongwen Yao [SMTP:zong-wen.yao-at-psi.ch]
Sent: Thursday, October 12, 2000 9:02 AM
To: Microscopy-at-sparc5.microscopy.com
Subject: copper eclectrical polishing


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Listers, I meet some problem how to electrical-polish 3mm pure
copper
disk. Would you plese tell me the good solution and the good
condition
of electrical-polishing copper on tenupol-5 machine.
Thanks

Mr.Zhongwen Yao
EPFL- CRPP
Villingen, PSI
Switzerland





From daemon Thu Oct 12 11:49:24 2000



From: Geoff McAuliffe :      mcauliff-at-UMDNJ.EDU
Date: Thu, 12 Oct 2000 12:49:05 -0400
Subject: Re: light microscope lenses

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Steve Barlow wrote:

} Netters
}
} In upgrading our light microscopes, we are buying some new lenses for our
} confocal scope and a brightfield scope. The salesforce is regaling us with
} tales of improved resolution, clarity, brightness etc that comes with
} spending double or triple the price for a standard oil immersion or dry
} lens. Could someone please clarify, either on-line or off, what the
} advantages and disadvantages of each of the following ( my texts on light
} microscopy don't deal with them). Are they worth the extra dollars?
}
} 1) a water immersion lens for looking at cells suspended in buffer and
} covered with a coverslip (water is used rather than oil between coverslip
} and lens) These lenses typically have a lower numerical aperture than an
} oil immersion lens.
}
} 2) a water dipping lens, used to examine cells in culture directly, without
} a coverslip. I would expect the absence of refractive indice changes
} (media/coverslip/air or oil) would explain their improved brightness.
}
} Thanks in advance for your input
}
} Steve Barlow
} SDSU EM Facility

So they are saying that all of their old lenses are junk?
Ask for a demo using your material at your site. Then you can see for yourself

Geoff

--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************





From daemon Thu Oct 12 11:56:13 2000



From: Gib Ahlstrand :      giba-at-puccini.cdl.umn.edu
Date: Thu, 12 Oct 2000 12:03:42 -0500 (CDT)
Subject: Re: questions for Pelco CPD2 users

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Responding to the message of
{Pine.GSO.4.10.10010111120030.19238-100000-at-aggie.pw.usda.gov}
from "Pauline C. Yu" {splene-at-pw.usda.gov} :
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.

} Hi listers
}
} We just bought a Pelco CPD2 with the hopes of replacing our old Polaron
} E3000, and so far, we're rather unimpressed just trying to get it through
} an initial setup.
} We were wondering if any other users could tell us how many runs they get
} out of an average CO2 cylinder? The cooling process with the CO2 seems
} somewhat inefficient at this point and we're using up a lot just trying to
} cool the chamber.

Pauline,

You should get very many runs out of an average cylinder. I'm not sure how the
Pelco is designed, but with my Ladd Company critical point dryer, also cooled by
CO2 gas flow-through, the "trick" is to set up a balance between the CO2 input
valve and the exhaust or drain valve such that you get a BIG pressure drop from
the CO2 tank pressure which is about 1000 psi - down to about 100-200 psi in the
chamber as the gas flows through. You shouldn't need to open the input valve
very much to achieve this effect. The cooling of the gas, hence the chamber,
depends on a big pressure drop, the ol' Joule-Thomposon effect, just like in a
refrigerator. When I first got my Ladd unit I had the same problem because I had
too high a pressure in the chamber during blow through of the gas.

I hope this helps. It should only take just a few minutes to cool the chamber
from room temp to about 5 C, for example, depending on size and mass of the
chamber on your unit

Good luck!

Gib.

}
} Thanks in advance,
}
} Pauline Yu
} splene-at-pw.usda.gov
} Microscopist Technician
} USDA-ARS-WRRC


Gib Ahlstrand
Electron Optical Facility, University of Minnesota, Dept. Plant Pathology
495 Borlaug Hall, St. Paul, MN. USA. 55108 (612)625-8249
612-625-9728 FAX, giba-at-puccini.cdl.umn.edu
http://biosci.umn.edu/MIC/consortium.html




From daemon Thu Oct 12 12:02:22 2000



From: David Henriks :      Henriks-at-CompuServe.COM
Date: Thu, 12 Oct 2000 12:59:43 -0400
Subject: copper electrical polishing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Mr. Yao:

I have looked in our archives and have found 2 solutions that Bernie Kestel
from Argonne National Lab has developed for copper which may be of use.
This work was done with our Model 550 single vertical jet electropolisher
so references to jet height would be unique to our system. The other
parameters may need to be adjusted slightly. Please be careful in using
any of these chemicals and especially when adjusting the recipes. I owuld
suggest that you contact Bernie Kestel directly prior to using these. You
can reach him by email at kestel-at-anl.gov.

Recipe #1
90% H3PO4
10% H2O

Temp: 20 degrees C
Jet height: 6.3mm
Pump setting: 8-10
Volts: 2
Current: 70mA

Recipe #2
150 ml HNO3
350 ml methanol
40 ml butyl cellosolve

Temp: -20 degrees C
Jet height: 3.9mm
Pump setting: 2.5
Volts: 40
Current: 75mA
NOTES: excellent polish. Lower temperature result in an uneven foil
surface.

I hope this information helps. If I can be of any additional assistance,
please feel free to contact me.

DISCLAIMER: South Bay Technology produces equipment and supplies as
described above and, therefore, has a vested interest in promoting their
use.

Best regards-

David
Writing at 9:44:06 AM on 10/12/2000

***************************************************************************
************************

David Henriks
Vice President TEL: 800-728-2233
(toll free in the USA)
South Bay Technology, Inc. +1-949-492-2600
1120 Via Callejon FAX: +1-949-492-1499
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

***************************************************************************
************************

} } } } } Please visit us at http://www.southbaytech.com { { { { {

Manufacturers of precision sample preparation equipment and supplies for
metallography, crystallography and electron microscopy.

Message text written by Zhongwen Yao
}
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Listers, I meet some problem how to electrical-polish 3mm pure copper
disk. Would you plese tell me the good solution and the good condition
of electrical-polishing copper on tenupol-5 machine.
Thanks

Mr.Zhongwen Yao
EPFL- CRPP
Villingen, PSI
Switzerland



{



From daemon Thu Oct 12 13:02:06 2000



From: Gareth Robinson :      Gareth.Robinson-at-emitech.co.uk
Date: Thu, 12 Oct 2000 18:47:30 +0100
Subject: RE: Coater for use with FESEM

Contents Retrieved from Microscopy Listserver Archives
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Very true,

The K575 uses rotary and turbomolecular drag pumping to achive the high
vaccum required.

The instrument has an automated shutter assembly to allow sputter target
cleaning (Remove the oxide layer from the chromium target material)
prior to sample sputtering.

We ahve had some very good application results witht he coater, please
drop me a line for further info.

Best regards

Gareth
} -----Original Message-----
} From: Tobias Baskin [SMTP:BaskinT-at-missouri.edu]
} Sent: Thursday, October 12, 2000 3:00 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: RE: Coater for use with FESEM
}
} ----------------------------------------------------------------------
} --
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------
} -.
}
}
} Greetings,
} As seen below, Gareth Robinson mentions chromium as an option
} for fine coats to use in high res SEM.
}
} A word of caution: Chromium is tricky to work with. You
} cannot use chromium sputter coating the same simply way you are used
} to sputtering with, say, gold or platinum. You have to keep the
} atmosphere in the coater squeaky clean, and even cleaner, and you
} must absolutely minimize the exposure of your coated sample to oxygen.
}
} I expect that chromium coats can be superb, but you cannot
} expect to simply put a chromium target in your sputter coater and
} have decent results. We found this out the hard way.
}
} As ever,
} Tobias
}
} }
} }
} }
} } Ben,
} }
} } You may also want to consider the option of Chromium coating which
} will
} } give grain siz of less than 0.5NM Chromium grain size with thin film
} } deposition typically 5NM.
} }
} } We manufacture and sell the K575 Sputter Coater unit. It has the
} } advantage of automated cycle coating Turbomolecular drag pumping for
} } High Vaccum.
} }
} } Please take a look at our web site for a detailed technical brief and
} } the instrument specification.
} }
} } HTTP://www.emitech.co.uk
} }
} } Kind regards
} }
} } Gareth Gareth.Robinson-at-emitech.co.uk
}
} --
} _ ____ __ ____ Tobias I. Baskin
} / \ / / \ / \ \ 109 Tucker Hall
} / / / / \ \ \ Biological
} Sciences
} /_ / __ /__ \ \ \__ University of
} Missouri
} / / / \ \ \ Columbia,
} MO USA
} / / / \ \ \ 65211-7400
} / / ___ / \ \__/ \ ____ voice:
} 573-882-0173
} fax: 573-882-0123



From daemon Thu Oct 12 13:02:06 2000



From: Buckingham, Steve :      sbuckingham-at-excellatron.com
Date: Thu, 12 Oct 2000 13:58:30 -0400
Subject: Concrete slab for SEM

Contents Retrieved from Microscopy Listserver Archives
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Hi All,
I have a JSM 840 that I need to relocate soon. The new location is
very near a railroad and interstate! Does anyone have information on the
optimum thickness of a concrete slab to prevent vibrations?
Thanks,

Steve Buckingham
Excellatron Solid State LLC
1460 Roswell Street, Suite J
Smyrna, GA 30080
phone 770 438 2201
fax 770 438 2118






From daemon Thu Oct 12 13:51:35 2000



From: Bruce Brinson :      brinson-at-ece.rice.edu
Date: Thu, 12 Oct 2000 13:50:24 -0500
Subject: TEM aperture Etching oops

Contents Retrieved from Microscopy Listserver Archives
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Hi E-beam buddies,
After our last excimer laser was shut down, I had the idea of
cleaning our Pt apertures with our plasma etcher using dry air.
Optically they appear cleaner but in the TEM they are worse than before
they went into the plasma. The aperture ID edge appears to be populated
with hemispherical features that are prone to charging.
I considered back streaming from the fomblin filled mech. pump but
there is no residual smell of oil or sign of it anywhere about the
chamber. Does anyone have any idea what is going on & perhaps how these
apertures might be recovered? Some are cheap some are not.

thanks,
Bruce Brinson
Rice U.




From daemon Thu Oct 12 14:20:10 2000



From: Kristen Lennon :      kalen-at-iastate.edu
Date: Thu, 12 Oct 2000 14:16:42 -0500
Subject: active oxygen species localization

Contents Retrieved from Microscopy Listserver Archives
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Hi All,
My goal is to localize active oxygen species/peroxidase activity in roots,
and am wondering if anyone out there has any advice. I have several
protocols, some using very different ways of utilizing DAB, and some using
cerium chloride. They range from using whole mount tissue to processing
tissue for TEM (and actually nothing in between). I am currently trying one
of the DAB protocols, but would greatly appreciate hearing from anyone who
has some insight.
Thanks again for your help,
Kristen
Kristen A. Lennon
Department of Plant Pathology
351 Bessey Hall
Iowa State University
Ames, IA 50011
kalen-at-iastate.edu




From daemon Thu Oct 12 14:59:44 2000



From: COURYHOUSE-at-aol.com
Date: Thu, 12 Oct 2000 15:55:46 EDT
Subject: Re: light microscope lenses

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Funny thing is, even some really old lenses (old brass ones)! When it comes
to low power still work pretty good!

I think many of the newer faster better sometimes what we will notice is
very slight, but sales forces tend to extol the fact we need the latest even
when perhaps we don't. i think anything produced today will only be just a
little bit if at all better than the stuff form the 80's

Now on electron microscopes things are a bunch diff. with age especially on
that old rust bucket of the edax computer We have on the AMR 1000... I
suppose I should gather some spares or just consider replacing it with a PC
when we finally get it set up and operational.

Ed Sharpe archivist for SMECC

{ { Subj: Re: light microscope lenses
Date: 10/12/00 12:21:14 PM US Mountain Standard Time
From: mcauliff-at-UMDNJ.EDU (Geoff McAuliffe)
To: sbarlow-at-sunstroke.sdsu.edu (Steve Barlow)
CC: CONFOCAL-at-LISTSERV.ACSU.BUFFALO.EDU, microscopy-at-sparc5.microscopy.com

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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-----------------------------------------------------------------------.


Steve Barlow wrote:

} Netters
}
} In upgrading our light microscopes, we are buying some new lenses for our
} confocal scope and a brightfield scope. The salesforce is regaling us with
} tales of improved resolution, clarity, brightness etc that comes with
} spending double or triple the price for a standard oil immersion or dry
} lens. Could someone please clarify, either on-line or off, what the
} advantages and disadvantages of each of the following ( my texts on light
} microscopy don't deal with them). Are they worth the extra dollars?
}
} 1) a water immersion lens for looking at cells suspended in buffer and
} covered with a coverslip (water is used rather than oil between coverslip
} and lens) These lenses typically have a lower numerical aperture than an
} oil immersion lens.
}
} 2) a water dipping lens, used to examine cells in culture directly, without
} a coverslip. I would expect the absence of refractive indice changes
} (media/coverslip/air or oil) would explain their improved brightness.
}
} Thanks in advance for your input
}
} Steve Barlow
} SDSU EM Facility

So they are saying that all of their old lenses are junk?
Ask for a demo using your material at your site. Then you can see for
yourself

Geoff

--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************


} }



From daemon Thu Oct 12 15:55:14 2000



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Thu, 12 Oct 2000 13:50:59 -0700
Subject: Re: copper eclectrical polishing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Mr. Yao,
The Struers Tenupol instructions I have recommend a solution for Cu
polishing consisting of:
250 ml phosphoric acid
500 ml distilled water
250 ml ethanol
2 ml Vogel's Sparbeize
5 g urea
"to be mixed successively" and used at 15 degrees C, 0.3 A current, 15 V,
flowrate 4 for 1 minute. I'm afraid I have no idea what "Vogel's Sparbeize" is.
Another solution I have used for the window technique is 33% (v/v) nitric
acid and 67% methanol at room temperature ( {25 degrees C), 8 V. You must
remove the sample quickly to prevent oxidation and wash in methanol. This is
a very flammable and reactive mixture used as jet fuel, so keep it away from
heat, sparks and flame.
50 ml At 08:02 AM 10/12/00 -0500, you wrote:
}
} Listers, I meet some problem how to electrical-polish 3mm pure copper
} disk. Would you plese tell me the good solution and the good condition
} of electrical-polishing copper on tenupol-5 machine.
} Thanks
}
} Mr.Zhongwen Yao

Good luck,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca




From daemon Thu Oct 12 16:03:24 2000



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Thu, 12 Oct 2000 14:00:19 -0700
Subject: Re: Inspection of "very thin" Au-films[PVD] by FESEM?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Gunnar,
I would recommend masking a part of the Ge by putting a bit of Al foil over
it before coating. You should be able to see the interface between the
coated and uncoated surface clearly in the FESEM due to the atomic weight
difference, and then zoom up to high magnification to see if there is any
structure in the Au film. I know in a Au sputtered film this could be seen
easily, but I haven't looked at a thermal evaporated film. It will show up
best on a polished Ge wafer. You can use higher kV (15-20) and high
resolution conditions in the FESEM, because the sample is conductive.
At 03:58 PM 10/12/00 +0200, you wrote:
} Hi,
}
} I am looking for help concerning the following problem: Someone in our
} institute is trying to deposit a 10nm Au-film on a Ge single crystal by
} thermal evaporation from a tungsten boat (...rate: 0.5A/s).
} The main point to me is, if FESEM is an appropriate method to determine if
} we
} have really a Au-film or only gold islands ...
}
} I do appreciate any comments concering both points (FESEM-tricks,
} because 99% of our samples are uncoated insulators) and the depostition
} technique/parameters for very thin gold films ...
}
} ThanX very much in advance!
}
}
}
} Dipl.Ing.(FH) G.Glasser

Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca




From daemon Thu Oct 12 17:40:58 2000



From: Gregory Mulhollan :      mulhollan-at-spec.com
Date: Thu, 12 Oct 2000 17:37:36 -0500
Subject: Data Translation board ouput pin connections

Contents Retrieved from Microscopy Listserver Archives
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MSA Listers,
This is a long shot, but if anyone of you has information on the pin output
of a DT2255 NuBus frame grabber and could forward to me off-list, I would be
very grateful. DT does not have this info on their web page and seems to be
selling support; I don't want to know how much they charge for pin output
info on obsolete boards! Thanks.

--
Gregory Mulhollan, Ph.D.
Extreme Devices Inc.
101 West 6th Street
Suite 200
Austin, TX 78701
(512)479-7740 x2231 voice
(512)479-7750 fax
mulhollan-at-extremedevices.com




From daemon Thu Oct 12 18:21:03 2000



From: Theresa Fassel :      tfassel-at-scripps.edu
Date: Thu, 12 Oct 2000 16:15:03 -0500
Subject: EM analysis of liposomes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello

I would appreciate any advice/recommendations on TEM analysis of lipsomes,
particularly vesicle size.
Thanks
Theresa


Theresa A. Fassel
Core EM Unit, Mail Box MB-32
The Scripps Research Institute
10,550 N. Torrey Pines Rd.
LaJolla, CA 92037-1027
tel: (858)-784-8182
fax: (858)-784-8193
tfassel-at-scripps.edu



From daemon Thu Oct 12 23:41:14 2000



From: Ian Cotton :      icotton-at-gatan.com
Date: Thu, 12 Oct 2000 23:33:49 -0500
Subject: Job openings at Gatan

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Product Manager,

Analytical Instruments

Gatan Inc. has an immediate opening for a person to handle GIF and PEELS
Product management. Gatan has a complete facility equiped with a new FEI
Tecnai 20,
a JEOL 2010/FasTEM and a Philips CM12.

The Individual should have either GIF or PEELS experience, a
strong TEM background in Biological or Materials science, good computer
skills, a willingness to travel and the vision and drive to help determine
the future of these products.

The position is based in Pleasanton, CA and
carries a salary comensurate with experience as well as a bonus plan and
Corporate success share plan.

Please contact Ian Cotton, Director of
Marketing at 925-224-7343 or email your resume to icotton-at-gatan.com.

*********************************
Ian Cotton
Marketing Director,
Gatan, Inc. 5933 Coronado Lane
Pleasanton, CA 94588 USA
Phone 925-224-7343
Fax 925-463-0204
E-mail icotton-at-gatan.com
Surf the Web to www.gatan.com
*********************************





From daemon Thu Oct 12 23:41:14 2000



From: Ian Cotton :      icotton-at-gatan.com
Date: Thu, 12 Oct 2000 23:34:24 -0500
Subject: Job openings at Gatan

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Applications Manager,

Gatan Inc. has an immediate opening for a person to manage our newly equiped
demonstration facility. We have recently installed a new FEI Tecnai 20 and a
JEOL 2010/FasTEM TEM. A full suite of Gatan analytical systems ( GIF and
ENFINA PEELS ) and imaging systems are installed on these two microscopes.

The Individual should have either GIF or PEELS experience, a
strong TEM background in Biological or Materials science, good computer
skills, and excellent interpersonal skills. You should be experienced in the
management of TEM instrumentation and have good organisational skills.

This is a challenging position for an individual who wants to work with
state of the art equipment and contribute to the future development of our
products.

The position is based in Pleasanton, CA and carries a salary comensurate
with experience as well as a bonus plan and Corporate success share plan.

Please contact Ian Cotton, Director of Marketing at 925-224-7343 or email
your resume to icotton-at-gatan.com.

*********************************
Ian Cotton
Marketing Director,
Gatan, Inc. 5933 Coronado Lane
Pleasanton, CA 94588 USA
Phone 925-224-7343
Fax 925-463-0204
E-mail icotton-at-gatan.com
Surf the Web to www.gatan.com
*********************************





From daemon Fri Oct 13 02:11:07 2000



From: Ruth Yamawaki :      Ryamawaki-at-cmexchange.stanford.edu
Date: Fri, 13 Oct 2000 00:02:46 -0700
Subject: EM tissue processor

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


A colleague of mine is interested in purchasing a tissue processor. She has
limited access to email so please reply directly to me and let me know what
processor you use and would recommend. I'll pass the word along.

Thank you,

Ruth

***************************************
Ruth Yamawaki
Department of Comparative Medicine
Stanford University
Stanford, CA 94305
***************************************




From daemon Fri Oct 13 03:54:25 2000



From: Witold Zielinski :      WIZIEL-at-inmat.pw.edu.pl
Date: Fri, 13 Oct 2000 10:56:41 +0000
Subject: Re: copper eclectrical polishing

Contents Retrieved from Microscopy Listserver Archives
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*Date sent: Thu, 12 Oct 2000 08:02:18 -0500
*To: Microscopy-at-sparc5.microscopy.com
*From: Zhongwen Yao {zong-wen.yao-at-psi.ch}
*Subject: copper eclectrical polishing

*------------------------------------------------------------------------
*The Microscopy ListServer -- Sponsor: The Microscopy Society of America

It seems that the best way, if you have enough money, is to buy
proper electrolyte from the Struers.
Based on our experiance, copper is difficult for TEM preparation.
We use to say that even weather has an influance on electropolishing
conditions.
Good luck,
Witold Zielinski

%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
Witold Zielinski, Ph.D.
Warsaw University of Technology
Department of Materials Science and Engineering
02-507 Warszawa, Woloska 141
POLAND

phone #: /48 22/ 660 84 41
fax #: /48 22/ 848 48 75



From daemon Fri Oct 13 05:07:20 2000



From: Zhongwen Yao :      zong-wen.yao-at-psi.ch
Date: Fri, 13 Oct 2000 12:03:32 +0400
Subject: Thanks

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Thank all of you who give me help very much!
Best Regards Mr.Z.Yao




From daemon Fri Oct 13 06:59:29 2000



From: Ken Converse :      qualityimages-at-netrax.net
Date: Fri, 13 Oct 2000 07:52:36 -0700
Subject: Re: Stub replacement

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Patton, David wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
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} -----------------------------------------------------------------------.
}
} We slice off the specimen with a razor blade and use the
} blade to scrape the stub fairly clean. As we use colloidal
} graphite, which is conductive, I do not mind some residual
} glue as it will get covered by glue from the next use.
} This glue can be removed, if required, by sonicating in
} butyl acetate.
}
} Dave
}
} On Wed, 11 Oct 2000 21:09:07 +1000 Jim at ProSciTech
} {jim-at-proscitech.com} wrote:
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
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} } -----------------------------------------------------------------------.
} }
} }
} } It all depends: how busy you are, how expensive staff time in your country is
} } and how costly the place is you buy your stubs from. Obviously some specimens
} } should be stored in a desiccator for an eternity and other can be discarded
} } immediately.
} } There is nothing magical about cleaning stubs. Solvents are messy. Best to
} } place on some newspaper a very coarse file or a sheet of coarse (50 grit)
} } sandpaper and then rub the old stub over that file of sandpaper until specimen
} } and glue are removed.
} }
} } Disclaimer: ProSciTech sells stubs, but we are happy if people recycle and this
} } leaves some money for useful purchases.
} } Cheers
} } Jim Darley
} } ProSciTech Microscopy PLUS
} } PO Box 111, Thuringowa QLD 4817 Australia
} } Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
} } Great microscopy catalogue, 500 Links, MSDS, User Notes
} } ABN: 99 724 136 560 www.proscitech.com
} }
} } On Wednesday, October 11, 2000 3:09 AM, O. O. Ilori
} } [SMTP:sojilori-at-oauife.edu.ng] wrote:
} } }
} } } Hello Guys
} } } I am a novice user of a SEM (LEO 1450 to be precise) equipment which was
} } } just installed in our central science lab.
} } } Is there a way to clean stubs for reuse or are stubs disposable once used.
} } } ( This may sound naive)
} } } soji
} } } Mr. O. O. ILORI
} } } DEPARTMENT OF ELECTRONIC/ELECTRICAL ENGINEERING
} } } OBAFEMI AWOLOWO UNIVERSITY,
} } } ILE-IFE, OSUN STATE
} } } NIGERIA.
} } } email: sojilori-at-oauife.edu.ng
} } }
} } }
} }
} }
}
} ----------------------------------------
} Patton, David
} Email: David.Patton-at-uwe.ac.uk
} "University of the West of England"
If you have access to a glass bead blaster, it does a great job as long
as you weren't looking for a mirror finish.

Ken Converse
owner
Quality Images
third party SEM service
Delta, PA



From daemon Fri Oct 13 07:17:21 2000



From: Ken Converse :      qualityimages-at-netrax.net
Date: Fri, 13 Oct 2000 08:12:37 -0700
Subject: Re: TEM aperture Etching oops

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Bruce Brinson wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hi E-beam buddies,
} After our last excimer laser was shut down, I had the idea of
} cleaning our Pt apertures with our plasma etcher using dry air.
} Optically they appear cleaner but in the TEM they are worse than before
} they went into the plasma. The aperture ID edge appears to be populated
} with hemispherical features that are prone to charging.
} I considered back streaming from the fomblin filled mech. pump but
} there is no residual smell of oil or sign of it anywhere about the
} chamber. Does anyone have any idea what is going on & perhaps how these
} apertures might be recovered? Some are cheap some are not.
}
} thanks,
} Bruce Brinson
} Rice U.
Bruce,
I've been cleaning apertures of all types for many years with 1 micron
diamond paste and a cut-knap polishing cloth, although any lint-free
cloth will work if the apertures are flat and fairly thin. Heavily
counter-sunk apertures like Siemens 2mm really benefit from the cut-knap
cloths (metallography supplies).

Finish by cleaning ultrsonically in Joy and hot water, blow dry
immediatly.

As to what is going on in your plasma system, I can't help you there.

Ken Converse
Quality Images
third party SEM service
Delta, PA



From daemon Fri Oct 13 07:42:16 2000



From: Brian McIntyre :      mcintyre-at-optics.rochester.edu
Date: Fri, 13 Oct 2000 08:32:34 -0400
Subject: Re: saw-tooth scan distortion: jeol35

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hi listers-


thanks for all your help in diagnosing my 35 problem. turns that the
aperture heater was the culprit. i'll make note of this for "next time"!

b-
----------------------------------------------
Brian McIntyre
Electron Microscopy Lab- River Campus
Univ of Rochester
Rochester, NY 14627
716-275-3058/4875



From daemon Fri Oct 13 07:52:17 2000



From: joe fu :      jofu-at-nist.gov
Date: Fri, 13 Oct 2000 08:48:56 -0400
Subject: Image analysis training course

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MSA Listers:
Is John Russ offering training course on image analysis this year? When?
Where? TIA.

Joseph Fu
National Institute of Standards & Technology
100 Bureau drive Stop 8212
Gaithersburg, MD. 20899-8212
Tel: 301-975-3495
Fax: 301-869-0822
Email: jofu-at-nist.gov





From daemon Fri Oct 13 07:53:29 2000



From: Gary Radice :      gradice-at-richmond.edu
Date: Fri, 13 Oct 2000 08:51:07 -0400
Subject: re: light microscope lenses

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I've had some experience with water immersion lenses. Their main
advantage is higher numerical aperture than dry lenses at equivalent
magnification, so better theoretical resolution. They don't offer
quite as good resolution as oil immersion lenses, but they tend to
have longer working distances, I believe, and don't have the messy
clean-up of oil immersion lenses. So, yes, they do have some distinct
advantages.

However, as others have pointed out, you may already be able to see
everything you need to see with your current lenses. And nothing
beats a test-drive.
--
Gary P. Radice gradice-at-richmond.edu
Associate Professor of Biology 804 289 8107 (voice)
University of Richmond 804 289 8233 (FAX)
Richmond VA 23173 http://www.science.richmond.edu/~radice



From daemon Fri Oct 13 08:29:54 2000



From: DrJohnRuss-at-aol.com
Date: Fri, 13 Oct 2000 09:22:11 EDT
Subject: Re: Image analysis training course

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In a message dated 10/13/00 9:01:02 AM, jofu-at-nist.gov writes:

} Is John Russ offering training course on image analysis this year? When?
} Where? TIA.

Thanks for the chance to plug the courses. There will be three sessions in
2001. Dates are May 9-11 and October 30-Nov. 1, in Raleigh (at N. C. State
Univ) and June 6-8 in Denmark (at the Danish Technological Institute). Full
info on the course contents, registration info, etc., (and a downloadable
copy of the brochure for the 2000 courses, which is correct except that it
does not have the year 2001 dates) is available on-line at
http://members.aol.com/IPCourse/

It is also possible (and cost effective for groups of more than about 6-7
people) to arrange for an on-site course. Contact Cindy Allen in the NCSU
Continuing Education department at 919 515 8171 for details.

John C. Russ
John_Russ-at-NCSU.edu



From daemon Fri Oct 13 10:11:35 2000



From: Earl Weltmer :      earlw-at-pacbell.net
Date: Fri, 13 Oct 2000 07:49:59 -0700
Subject: Re: Concrete slab for SEM

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Hi Steve,

I don't think there is an "optimum" thickness for the concrete slab.
If vibration from the railroad (or any other source) is a concern most isolate
the main slab by sawing & removing the concrete under the optics console. A
new concrete slab is laid with "pea sized agregate" & felt on the sides. This
lets the column stand free from the rest of the slab.

Regards,

Earl Weltmer

"Buckingham, Steve" wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hi All,
} I have a JSM 840 that I need to relocate soon. The new location is
} very near a railroad and interstate! Does anyone have information on the
} optimum thickness of a concrete slab to prevent vibrations?
} Thanks,
}
} Steve Buckingham
} Excellatron Solid State LLC
} 1460 Roswell Street, Suite J
} Smyrna, GA 30080
} phone 770 438 2201
} fax 770 438 2118




From daemon Fri Oct 13 11:05:22 2000



From: Wil Bigelow :      bigelow-at-engin.umich.edu
Date: Fri, 13 Oct 2000 11:01:49 -0400
Subject: RE: Cleaning Pt Apertures

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Many years ago we regularly used platinum crucibles in many analytical
chemistry procedures. According to my analytical chemistry book, platinum
can be cleaned by fusion with potassium or sodium bisulfite. I later used
this procedure with good success to clean the platinum apertures for an RCA
EML electron microscope. Get a small ceramic crucible, put a bit of the
bisulfite in it, heat it until the bisulfite melts, then drop the apertures
into the melt. After a few minutes allow the melt to solidify, dissolve
the bisulfite with hot water, and your apertures should be as bright and
shiny as new.

If this doesnn't work, try heating them in a mixture of equal parts of
concentrated hydrofluoric acid and concentrated hydrochloric acid - using
appropriate safety precautions, of course!

Good luck

Wilbur C. Bigelow, Prof. Emeritus
Materials Sci. & Engr., University of Michigan
Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu;
Fx:734-763-4788; Ph:734-662-5237





From daemon Fri Oct 13 11:32:33 2000



From: M. T. Coating :      mtcoating-at-meyertool.com
Date: Tue, 10 Oct 2000 09:48:04 -0400
Subject: microprobe

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To whom it may concern,
I am a materials engineering student at the Univ of Cincinnati (Ohio)
currently co-oping at a local aerospace company. They're wanting some
microprobe analysis done on some parts and have put me in charge of that.
I have a descent understanding of X-Ray diffraction but would like some
information on the specifics of a microprobe so that I know exactly what is
going on when I'm sitting watching the analysis take place. Is there any
electronic information you can send or any sites you can refer me to that
will give in depth information on the workings of a microprobe? Please
respond to keithj-at-meyertool.com.

Thank You
Keith Jones




From daemon Fri Oct 13 11:55:08 2000



From: Ladd Research :      ladres-at-worldnet.att.net
Date: Fri, 13 Oct 2000 12:49:21 -0400
Subject: Re: TEM aperture Etching oops

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Bruce,

Based on your information we believe there may be burrs on the ID edge
of your holes.
We could open up the holes a bit to provide burr-free apertures, or we
would allow you a trade in since as the only US Electron Microscope
aperture manufacturer they probably originated with us anyway.
If an aperture has a burr-free edge, a flamer should do for cleaning Pt
and if they are Moly you can clean them in an evaporator.
Even if your aperture is imported we would allow a trade in on a new
disc or strip.
Let me know if I can be of further help,

John Arnott
--

LADD RESEARCH
131 Dorset Lane
Williston, VT 05495 USA

web site http://www.laddresearch.com

TEL 1-800-451-3406 (US) or 1-802-878-6711 (anywhere)
FAX 1-802-878-8074
e-mail sales-at-laddresearch.com

Quality Since 1955

Bruce Brinson wrote:
}

} Hi E-beam buddies,
} After our last excimer laser was shut down, I had the idea of
} cleaning our Pt apertures with our plasma etcher using dry air.
} Optically they appear cleaner but in the TEM they are worse than before
} they went into the plasma. The aperture ID edge appears to be populated
} with hemispherical features that are prone to charging.
} I considered back streaming from the fomblin filled mech. pump but
} there is no residual smell of oil or sign of it anywhere about the
} chamber. Does anyone have any idea what is going on & perhaps how these
} apertures might be recovered? Some are cheap some are not.
}
} thanks,
} Bruce Brinson
} Rice U.



From daemon Fri Oct 13 12:34:02 2000



From: Gib Ahlstrand :      giba-at-puccini.cdl.umn.edu
Date: Fri, 13 Oct 2000 12:36:37 -0500 (CDT)
Subject: Needles

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Mark,

Any feedback on the SEM images of the needles? Should I go ahead and finish the
other 4 that way?

At only 25X mag, seems like LM would give better images, in color, for example,
and the steel surfaces seem to show up better. The SEM images look a bit gray,
as no metal coating and low kV imaging. Metal coating would show them up better,
but I suppose that would not be allowed under the experimental constraints.

What do you think?

Gib

Gib Ahlstrand
Electron Optical Facility, University of Minnesota, Dept. Plant Pathology
495 Borlaug Hall, St. Paul, MN. USA. 55108 (612)625-8249
612-625-9728 FAX, giba-at-puccini.cdl.umn.edu
http://biosci.umn.edu/MIC/consortium.html




From daemon Fri Oct 13 13:19:12 2000



From: Matthew Lynn :      mlynn-at-miami.edu
Date: Fri, 13 Oct 2000 14:14:22 -0400
Subject: RE: TEM aperture Etching oops

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Hi Bruce,
}
} I have never tried the plasma etcher but I suspect it would remove organics
} effectively, just as a flame does. After several cleanings in the flame, we
} are usually left with deposits similar to what you describe. My old Philips
} 300 manual suggests these are usually silica-- this makes sense for our
} samples, maybe for yours as well? Same manual advises "24 hrs. immersion in
} 10% solution of HF in water". I usually save the ones I can't clean until I
} have several, then turn them over to a colleague who works with HF regularly.

} Most come back as clean as new. The hemispherical features suggest to me
that
} our contaminant will melt, but not vaporize in the flame, consistent with
} silica. You would have to look into the power of your etcher. Naturally,
} these techniques are for solid Pt apertures, and HF is not for the
} accident-prone. Good luck! By the way, our much newer Philips manual (XL30
} ESEM-FEG) gives 4 methods of heating but no mention of HF.
}
} Matt
}


Matthew J. Lynn, Ph.D.
Center for Advanced Microscopy
University of Miami
(305)284-4736
mlynn-at-miami.edu




From daemon Fri Oct 13 13:56:21 2000



From: Sue Barnes :      SBarnes-at-elch.org
Date: Fri, 13 Oct 2000 14:50:38 -0400
Subject: subscribe

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From daemon Fri Oct 13 14:27:59 2000



From: David Cockayne :      david.cockayne-at-materials.oxford.ac.uk
Date: Fri, 13 Oct 2000 20:17:33 +0100
Subject: Fw: Concrete slab for SEM

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Earl

The optimum thickness of concrete is whatever thickness of concrete it
takes to stop the train. Any other answer is incorrect.


David C

}
} ----- Original Message -----
} From: Earl Weltmer {earlw-at-pacbell.net}
} To: Buckingham, Steve {sbuckingham-at-excellatron.com}
} Cc: 'Microscopy-at-MSA.Microscopy.Com' {Microscopy-at-sparc5.microscopy.com}
} Sent: Friday, October 13, 2000 3:49 PM
} Subject: Re: Concrete slab for SEM
}
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Hi Steve,
} }
} } I don't think there is an "optimum" thickness for the concrete slab.
} } If vibration from the railroad (or any other source) is a concern most
} isolate
} } the main slab by sawing & removing the concrete under the optics
console.
} A
} } new concrete slab is laid with "pea sized agregate" & felt on the sides.
} This
} } lets the column stand free from the rest of the slab.
} }
} } Regards,
} }
} } Earl Weltmer
} }
} } "Buckingham, Steve" wrote:
} }
} }
} ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
} } } To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
} } } On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} }
} -----------------------------------------------------------------------.
} } }
} } } Hi All,
} } } I have a JSM 840 that I need to relocate soon. The new
location
} is
} } } very near a railroad and interstate! Does anyone have information on
the
} } } optimum thickness of a concrete slab to prevent vibrations?
} } } Thanks,
} } }
} } } Steve Buckingham
} } } Excellatron Solid State LLC
} } } 1460 Roswell Street, Suite J
} } } Smyrna, GA 30080
} } } phone 770 438 2201
} } } fax 770 438 2118
} }
} }
} }
}




From daemon Fri Oct 13 14:40:45 2000



From: David Cockayne :      david.cockayne-at-materials.oxford.ac.uk
Date: Fri, 13 Oct 2000 20:29:13 +0100
Subject: Concrete slab

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Earl

The optimum thickness of concrete is whatever thickness of concrete it
takes to stop the train. Any other answer is incorrect.


David C

}
} ----- Original Message -----
} From: Earl Weltmer {earlw-at-pacbell.net}
} To: Buckingham, Steve {sbuckingham-at-excellatron.com}
} Cc: 'Microscopy-at-MSA.Microscopy.Com' {Microscopy-at-sparc5.microscopy.com}
} Sent: Friday, October 13, 2000 3:49 PM
} Subject: Re: Concrete slab for SEM
}
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Hi Steve,
} }
} } I don't think there is an "optimum" thickness for the concrete slab.
} } If vibration from the railroad (or any other source) is a concern most
} isolate
} } the main slab by sawing & removing the concrete under the optics
console.
} A
} } new concrete slab is laid with "pea sized agregate" & felt on the sides.
} This
} } lets the column stand free from the rest of the slab.
} }
} } Regards,
} }
} } Earl Weltmer
} }
} } "Buckingham, Steve" wrote:
} }
} }
} ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
} } } To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
} } } On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} }
} -----------------------------------------------------------------------.
} } }
} } } Hi All,
} } } I have a JSM 840 that I need to relocate soon. The new
location
} is
} } } very near a railroad and interstate! Does anyone have information on
the
} } } optimum thickness of a concrete slab to prevent vibrations?
} } } Thanks,
} } }
} } } Steve Buckingham
} } } Excellatron Solid State LLC
} } } 1460 Roswell Street, Suite J
} } } Smyrna, GA 30080
} } } phone 770 438 2201
} } } fax 770 438 2118
} }
} }
} }
}





From daemon Fri Oct 13 14:55:12 2000



From: Vitaly Feingold :      vitalylazar-at-worldnet.att.net
Date: Thursday, October 12, 2000 3:45 PM
Subject: Concrete slab for SEM

Contents Retrieved from Microscopy Listserver Archives
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Steve,

It is not the thickness, but rather the area of the slab. It is better be
minimal. Also, the soil under the slab- rock is bad, sand is good. If you
have any choice on the matter- construction is not started yet, etc.- than
have the slab on the sand cushion, make it small, just the size of the SEM
footprint, and separate from the rest of the building.
It is possible, in some cases, to cut the existing concrete slab in order to
separate it from the rest of the structure. (Consult the construction expert
first!!) Then, fill the cuts with soft silicone, rubber, etc. in order to
prevent hard objects from getting in the cuts, as they will make an acoustic
contact, which you are trying to avoid.

Also, many options from many sources are available regarding antivibration
supports and mounts, depending on your situation and requirements. Some are
expensive, some are not. Contact me off list if you need further help.

Vitaly Feingold
Scientific Instruments and Applications
2773 Heath Lane, Duluth GA 30096
(770)232-7785 ph.
(770)232-1791 fax

This message is made of 100% recycled electrons.
-----Original Message-----
} From: Buckingham, Steve {sbuckingham-at-excellatron.com}
To: 'Microscopy-at-MSA.Microscopy.Com' {Microscopy-at-sparc5.microscopy.com}




From daemon Fri Oct 13 15:10:47 2000



From: Earl Weltmer :      earlw-at-pacbell.net
Date: Fri, 13 Oct 2000 13:03:39 -0700
Subject: Re: Fw: Concrete slab for SEM

Contents Retrieved from Microscopy Listserver Archives
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Dave,

Have you considered the speed, size, & direction of the train?
In addition, the type of concrete & it's reinforcement material (ribar) also
needs to be considered.
Also whether you want the train to transmit vibration, collide with the SEM or
land on top of the SEM needs to be calculated.

Any other answer is incorrect.

Regards,

Earl

David Cockayne wrote:

} Earl
}
} The optimum thickness of concrete is whatever thickness of concrete it
} takes to stop the train. Any other answer is incorrect.
}
} David C
}
} }
} } ----- Original Message -----
} } From: Earl Weltmer {earlw-at-pacbell.net}
} } To: Buckingham, Steve {sbuckingham-at-excellatron.com}
} } Cc: 'Microscopy-at-MSA.Microscopy.Com' {Microscopy-at-sparc5.microscopy.com}
} } Sent: Friday, October 13, 2000 3:49 PM
} } Subject: Re: Concrete slab for SEM
} }
} }
} } } ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } -----------------------------------------------------------------------.
} } }
} } }
} } } Hi Steve,
} } }
} } } I don't think there is an "optimum" thickness for the concrete slab.
} } } If vibration from the railroad (or any other source) is a concern most
} } isolate
} } } the main slab by sawing & removing the concrete under the optics
} console.
} } A
} } } new concrete slab is laid with "pea sized agregate" & felt on the sides.
} } This
} } } lets the column stand free from the rest of the slab.
} } }
} } } Regards,
} } }
} } } Earl Weltmer
} } }
} } } "Buckingham, Steve" wrote:
} } }
} } }
} } ------------------------------------------------------------------------
} } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} } } } To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} } } } On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } }
} } -----------------------------------------------------------------------.
} } } }
} } } } Hi All,
} } } } I have a JSM 840 that I need to relocate soon. The new
} location
} } is
} } } } very near a railroad and interstate! Does anyone have information on
} the
} } } } optimum thickness of a concrete slab to prevent vibrations?
} } } } Thanks,
} } } }
} } } } Steve Buckingham
} } } } Excellatron Solid State LLC
} } } } 1460 Roswell Street, Suite J
} } } } Smyrna, GA 30080
} } } } phone 770 438 2201
} } } } fax 770 438 2118
} } }
} } }
} } }
} }




From daemon Fri Oct 13 15:48:24 2000



From: Earl Weltmer :      earlw-at-pacbell.net
Date: Fri, 13 Oct 2000 13:40:37 -0700
Subject: Ortec EDS detector

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All,

I have an recently rebuit Ortec EDS detector with electronics (old) from
a Zeiss TEM free to anyone.

If interested, please respond offline.

Earl Weltmer
Scanservice Corp.
(714) 573-9158




From daemon Fri Oct 13 15:56:57 2000



From: Frank Macaluso :      macaluso-at-aecom.yu.edu
Date: Fri, 13 Oct 2000 16:50:42 -0400
Subject: NYSEM Presidential Symposium 2000

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


NYSEM Presidential Symposium 2000: "Cell Polarization: Mechanisms at the
Membrane"

The New York Society for Experimental Microscopy will hold its annual
Presidential Symposium on 19 October from 9:00 AM to 5:30 PM at Columbia
University (Hammer Health Sciences Building, Room 401*). This year's
Symposium is entitled: "Cell Polarization: Mechanisms at the Membrane".
The Symposium will focus on recent breakthroughs in understanding the
molecular mechanisms involved in the generation of cell polarity.
Symposium participants will highlight recent advances in a number of model
systems in which microscopic approaches can be combined with genetic and
molecular biological approaches to understand cell polarity. One emerging
theme that will be explored during the Symposium is the degree to which
basic molecular pathways for cell polarization have been conserved despite
the diversity of structures that result in the different systems.
Registration is free, but preregistration is required for lunch (contact
Dr. Philip Leopold, Cornell Univ. Medical School, email:
pleopold-at-mail.med.cornell.edu; phone 212-746-2255)


* The Hammer Health Sciences Building is one block west of Broadway on the
corner of Fort Washington and 168th Street. There is a subway stop at
Broadway and 168th Street.


SCHEDULE - NYSEM PRESIDENTIAL SYMPOSIUM 2000
"Cell Polarization: Mechanism at the Membrane"
19 October 2000
Columbia University
Hammer Health Sciences Building, Room 401

8:30 - 9:00 On Site Registration* and Coffee

9:00 - 9:15 Gregg Gundersen (NYSEM President): Welcome and opening remarks

9:15 - 9:55 John Cooper (Department of Cell Biology & Physiology,
Washington University, St. Louis):
"Interactions of Microtubules with the Cortex During Mitosis in Budding
Yeast"

9:55 - 10:35 Kerry Bloom (Department of Biology, University of North
Carolina - Chapel Hill
"How Microtubules Polarize Nuclear Movements in Yeast"

10:35 - 10:55 Coffee Break

10:55 - 11:35 Fred Chang (Department of Microbiology, Columbia University,
New York)
"Spatial control and cytoskeletal dynamics in fission yeast"

11:35 - 12:15 Carole Parent (National Cancer Institute, NIH, Bethesda)
"Visualizing Signaling Events in Chemotaxing Eukaryotic Cells"

12:15 - 2:00 Lunch - Riverview Lounge (adjacent to Hammer 401)

2:00 - 2:40 Gregg Gundersen (Department of Anatomy & Cell Biology, Columbia
University, New York)
"Rho GTPase Pathways Integrate the Microtubule and Actin Cytoskeletons
During Cell Migration"

2:40 - 3:20 Clare Waterman-Storer (Department of Cell Biology, Scripps
Research Institute, La Jolla)
"Microtubule and Actin Interactions in Cell Polarization and Motility"

3:20 - 3:40 Coffee Break

3:40 - 4:20 Enrique Rodriguez-Boulan (Dyson Vision Research Institute,
Cornell University, New York)
"Role of Actin Filaments and Microtubules in Polarized Delivery of Plasma
Membrane Proteins"

4:20 - 4:30 Gregg Gundersen - Closing Remarks

4:30 - 5:30 Reception - River View Lounge



****************************************************************************
Frank Macaluso tel: 718-430-3547
Analytical Imaging Facility fax: 718-430-8996
Albert Einstein College of Medicine e-mail: macaluso-at-aecom.yu.edu
1300 Morris Park Avenue http://www.aecom.yu.edu/aif/
Bronx, NY 10461
****************************************************************************




From daemon Fri Oct 13 16:44:27 2000



From: Theresa Fassel :      tfassel-at-scripps.edu
Date: Fri, 13 Oct 2000 16:03:56 -0500
Subject: TEM of dynabeads

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


---------------------- Forwarded by Paula M Churt/TMG/CSC on 10/13/2000
10:59 AM ---------------------------


Diane A Ciaburri/GDDS-at-GDDS
10/13/2000 09:55 AM

To: Paula M Churt/TMG/CSC-at-CSC
cc:


Hello

Has anyone observed biological material-antibody-dynabead complex in EM?
Our user has great results with Dynabeads M-450 Epoxy Product # 140.01 in
his assay, but we would like to visualize the virus attached to the
dynabead. The manufacturer suggests the iron core may leak on sectioning
causing contamination. Any ideas?
Thanks

Theresa


Theresa A. Fassel
Core EM Unit, Mail Box MB-32
The Scripps Research Institute
10,550 N. Torrey Pines Rd.
LaJolla, CA 92037-1027
tel: (858)-784-8182
fax: (858)-784-8193
tfassel-at-scripps.edu



From daemon Fri Oct 13 18:20:05 2000



From: Gary Gaugler :      gary-at-gaugler.com
Date: Sat, 14 Oct 2000 01:33:22 -0700
Subject: Re: Cleaning of SEM Mag Standard

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Earl

The optimum thickness of concrete is whatever thickness of concrete it
takes to stop the train. Any other answer is incorrect.


David C


----- Original Message -----
} From: Earl Weltmer {earlw-at-pacbell.net}
To: Buckingham, Steve {sbuckingham-at-excellatron.com}
Cc: 'Microscopy-at-MSA.Microscopy.Com' {Microscopy-at-sparc5.microscopy.com}
Sent: Friday, October 13, 2000 3:49 PM


I run in the ETCH mode with Argon. Set power level to
5%. Put in the dummy Aluminum target. Hit PROCESS
and keep the vacuum at 60mTorr-40mTorr. Try for about 30
seconds and then look at the standard. Repeat until
clean. The lower the vacuum level, the less aggressive
is the cleaning action. The plasma will strike when the
vacuum reaches 20mTorr in the ETCH mode; 40mTorr
in the PLATE mode. When done, remove the Al target
and put back in the sputter coater target.

I coat (PLATE) at 80mTorr, 5nM/minute. This gives a
nice, smooth and even coating with Au/Pd or Pt.

I'm using a Hummer VII. BTW, if you have trouble with
the plasma not striking for PLATE when vacuum reaches
40mTorr, I have a simple fix that makes it virtually
never fail to fire.

gary g.


At 07:59 AM 10/13/00, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
}
} 10/13/00 Subject: Cleaning of SEM Mag
} Standard
} 09:36
} AM
}
}
}
}
}
}
}
}
} Hi all,
}
} I've got an NIST 484e SEM Mag Standard that has years of carbon build-up
} ("burn marks"). I want to clean it with my plasma etcher but I'm not sure
} about the parameters to use. Does anyone have experience doing this?
}
} I have an Anatech Hummer plasma coater. Do I use the etch or plasma mode?
} Argon or air for atmosphere? What vacuum and voltage, and for how long?
}
} Thanks!
}
} Diane Ciaburri
}
} dciaburri-at-gdds.com
} (413)494-2847
} General Dynamics
} 100 Plastics Ave.
} Pittsfield Ma 01201




From daemon Sat Oct 14 11:50:20 2000



From: Tamara Howard :      howard-at-cshl.org
Date: Sat, 14 Oct 2000 16:11:43 -0400 (EDT)
Subject: Re: TEM of dynabeads

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Steve,

David is right.
Our testing has shown that saw cutting the floor to isolate the column from
the rest of the building has no affect other than to cost a lot. The
vibration is transmitted through the ground, not the bldg.

Craig Franklin
Vibration Engineering Consultants
www.vibeng.com


----- Original Message -----
} From: David Cockayne {david.cockayne-at-materials.oxford.ac.uk}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Friday, October 13, 2000 5:16 PM


I don't know that the beads "leak", but they do not present a smooth
appearance in sections, either internally or - sometimes - at the surface.
the internal appearance can be discounted, but I always process some
control ("clean") beads in parallel. Sometimes there is a little fluffy
stuff (OK - flocculent material) on the surface of the blank beads, but it
could never (in my experience) be confused with viruses.

One other thing I would suggest is to have your researcher try to get as
much material aspossible on small beads. I once examined some IP samples
that were not very efficiently done on large beads - between the large
surface area of the beads and the low density of actual sample on the
beads, a *lot* of time was wasted just looking for the IP material. A real
aspirin project :)

I section Dynabeads on the not-so-great part of my diamond knife; I
haven't noticed new scratches from it, but I suspect that the material is
potentially dulling and scratchy.

Good luck!

Tamara Howard
CSHL


On Fri, 13 Oct 2000, Theresa Fassel wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hello
}
} Has anyone observed biological material-antibody-dynabead complex in EM?
} Our user has great results with Dynabeads M-450 Epoxy Product # 140.01 in
} his assay, but we would like to visualize the virus attached to the
} dynabead. The manufacturer suggests the iron core may leak on sectioning
} causing contamination. Any ideas?
} Thanks
}
} Theresa
}
}
} Theresa A. Fassel
} Core EM Unit, Mail Box MB-32
} The Scripps Research Institute
} 10,550 N. Torrey Pines Rd.
} LaJolla, CA 92037-1027
} tel: (858)-784-8182
} fax: (858)-784-8193
} tfassel-at-scripps.edu
}
}




From daemon Sat Oct 14 18:30:44 2000



From: Ronald Austin :      rla-at-mindspring.com
Date: Sat, 14 Oct 2000 18:25:00 -0500
Subject: EM tissue processor

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Ruth;
I would highly recommend the Ted Pella Microwave oven series 3450. I have
used this oven for over 3 years now and have processed over 500 specimens
(human and animal) without a single mishap to the tissue. In fact you may
find the results better then you would get by conventional processing. You
can contact Rick Giberson with Ted Pella's toll free number and he can give
you more specific information and a price to work with. You can contact me
at rla-at-mindspring.com as well.

Ron Austin (Research Associate)
LSU Medical Ct. at Shreveport
Dept. of Pathology
Shreveport, LA
318-675-4557





From daemon Sun Oct 15 12:15:12 2000



From: anderron-at-us.ibm.com
Date: Sun, 15 Oct 2000 13:04:43 -0400
Subject: Microscopy request from TV Show NOVA

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The following is a request from a producer of the PBS TV show NOVA, which
came in to the MSA Business office.

Can anyone help? Please correspond directly to the requester.

} } } } } }

Hi Folks --

I'm a producer with the science TV series NOVA. I'm working on a show
about evolution and I'm desperately looking for a way to adapt a zeiss
axiophot microscope to an Aaton 16mm film camera. So far I've struck
out on finding anyone who has gear to accomplish this feat.

Any suggestiongs?

--
Chris Schmidt
Powderhouse Productions
259 Elm Street
Somerville MA 02144
617-629-2200 work
617-776-7905 fax
781-820-0727 mobile
617-403-8699 pager
781-646-0928 home
Chris-at-powderhouse.net






From daemon Sun Oct 15 14:07:57 2000



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Mon, 16 Oct 2000 09:19:21 GMT+1200
Subject: Re: microprobe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Craig, David, Earl, Steve,

Increasing the mass under the microscope works. An isolated room-size
slab of 1m thickness can reduce vertical vibration by a factor of four
and horizontal vibration by 10 to 12 times [1]. This foundation was a
significant factor in allowing us the obtain a TEM resolution better
than one Angstrom [2].

-Mike O'Keefe

1. "Design and implementation of a site for a one-Ångstrom TEM", John H.
Turner, Michael A. O'Keefe and Robert Mueller, in 55th Ann. Proc. MSA,
Cleveland, Ohio (1997) 1177-1178.
2. "The NCEM One-Ångstrom Microscope Project Reaches 0.89Å Resolution",
Michael A. O'Keefe and Y.C. Wang in 58th Ann. Proc. MSA, Philadelphia,
Pennsylvania (2000).

----- Original Message -----
} From: "Craig Franklin" {franklin-at-vibeng.com}


Craig, David, Earl, Steve,

Increasing the mass under the microscope works. An isolated room-size
slab of 1m thickness can reduce vertical vibration by a factor of four
and horizontal vibration by 10 to 12 times [1]. This foundation was a
significant factor in allowing us the obtain a TEM resolution better
than one Angstrom [2].

-Mike O'Keefe

1. "Design and implementation of a site for a one-Ångstrom TEM", John H.
Turner, Michael A. O'Keefe and Robert Mueller, in 55th Ann. Proc. MSA,
Cleveland, Ohio (1997) 1177-1178.
2. "The NCEM One-Ångstrom Microscope Project Reaches 0.89Å Resolution",
Michael A. O'Keefe and Y.C. Wang in 58th Ann. Proc. MSA, Philadelphia,
Pennsylvania (2000).


----- Original Message -----
} From: "Craig Franklin" {franklin-at-vibeng.com}


Craig, David, Earl, Steve,

Increasing the mass under the microscope works. An isolated room-size
slab of 1m thickness can reduce vertical vibration by a factor of four
and horizontal vibration by 10 to 12 times [1]. This foundation was a
significant factor in allowing us the obtain a TEM resolution of better
than one Angstrom [2].

-Mike O'Keefe

1. "Design and implementation of a site for a one-Ångstrom TEM", John H.
Turner, Michael A. O'Keefe and Robert Mueller, in 55th Ann. Proc. MSA,
Cleveland, Ohio (1997) 1177-1178.
2. "The NCEM One-Ångstrom Microscope Project Reaches 0.89Å Resolution",
Michael A. O'Keefe and Y.C. Wang in 58th Ann. Proc. MSA, Philadelphia,
Pennsylvania (2000).


----- Original Message -----
} From: "Craig Franklin" {franklin-at-vibeng.com}


Craig, David, Earl, Steve,

Increasing the mass under the microscope works. An isolated room-size
slab of 1m thickness can reduce vertical vibration by a factor of four
and horizontal vibration by 10 to 12 times [1]. Such a foundation was a
significant factor in allowing us to obtain a TEM resolution better than
one Angstrom [2].

-Mike O'Keefe

1. "Design and implementation of a site for a one-Ångstrom TEM", John H.
Turner, Michael A. O'Keefe and Robert Mueller, in 55th Ann. Proc. MSA,
Cleveland, Ohio (1997) 1177-1178.
2. "The NCEM One-Ångstrom Microscope Project Reaches 0.89Å Resolution",
Michael A. O'Keefe and Y.C. Wang in 58th Ann. Proc. MSA, Philadelphia,
Pennsylvania (2000).



----- Original Message -----
} From: "Craig Franklin" {franklin-at-vibeng.com}


Craig, David, Earl, Steve,

Increasing the mass under the microscope works. An isolated room-size
slab of 1m thickness can reduce vertical vibration by a factor of four
and horizontal vibration by 10 to 12 times [1]. Such a foundation was a
significant factor in allowing us to obtain a TEM resolution better than
one Angstrom [2].

-Mike O'Keefe

1. "Design and implementation of a site for a one-Ångstrom TEM", John H.
Turner, Michael A. O'Keefe and Robert Mueller, in 55th Ann. Proc. MSA,
Cleveland, Ohio (1997) 1177-1178.
2. "The NCEM One-Ångstrom Microscope Project Reaches 0.89Å Resolution",
Michael A. O'Keefe and Y.C. Wang in 58th Ann. Proc. MSA, Philadelphia,
Pennsylvania (2000).



----- Original Message -----
} From: "Craig Franklin" {franklin-at-vibeng.com}



} I have a descent understanding of X-Ray diffraction but would like some
} information on the specifics of a microprobe so that I know exactly what is
} going on when I'm sitting watching the analysis take place. Is there any
} electronic information you can send or any sites you can refer me to that
} will give in depth information on the workings of a microprobe?

Keith

There is a wonderful book "Handbook of Silicate Analysis", by P J
Potts, that has great chapters on many modern instrumental
techniques, including XRF and EPMA.
Although written with a geological slant, Potts combines lucidity
with solid info, and it should be interesting to you also.

cheers

rtch

ps I hope your XRD is located downstairs.


Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand



From daemon Sun Oct 15 22:42:16 2000



From: sfield-at-island.net ()
Date: Mon, 16 Oct 2000 08:36:55 -0500
Subject: Ask-A-Microscopist: Recommendation needed booklet that will give

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ritchie,

Thanks for the heads-up. I was working from home this morning (Sunday)
and finally noticed and stopped it. I hoped it had only gone out once
or twice, but I had received five copies this afternoon when I checked
the listserver. :-(

Cheers,
Mike

----- Original Message -----
} From: "Ritchie Sims" {r.sims-at-auckland.ac.nz}


To Mike, Craig, Earl and others,

Many years ago there was an article in JEOL News detailing the
design of floors for electron microscopes at the John Innes Institute
in the UK, and more recently I saw similarly detailed plans for a
new facility in Australia (in Brisbane, I think). We have incorporated
here aspects of the John Innes Institute plans, and do not have a
vibration problem, but as we do not have trains that run anywhere
nearby, unfortunately I have no idea whether or not these floors
would be effective in reducing the vibration being discussed!

Rob

On 15 Oct 00, at 19:03, MAOKeefe-at-lbl.gov wrote:

} ----------------------------------------------------------------------
} -- The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------
} -.
}
}
} Craig, David, Earl, Steve,
}
} Increasing the mass under the microscope works. An isolated room-size
} slab of 1m thickness can reduce vertical vibration by a factor of four
} and horizontal vibration by 10 to 12 times [1]. This foundation was a
} significant factor in allowing us the obtain a TEM resolution better
} than one Angstrom [2].
}
} -Mike O'Keefe
}
} 1. "Design and implementation of a site for a one-Ångstrom TEM", John
} H. Turner, Michael A. O'Keefe and Robert Mueller, in 55th Ann. Proc.
} MSA, Cleveland, Ohio (1997) 1177-1178. 2. "The NCEM One-Ångstrom
} Microscope Project Reaches 0.89Å Resolution", Michael A. O'Keefe and
} Y.C. Wang in 58th Ann. Proc. MSA, Philadelphia, Pennsylvania (2000).
}
} ----- Original Message -----
} } From: "Craig Franklin" {franklin-at-vibeng.com}
} Date: Saturday, October 14, 2000 8:11 am
} Subject: Re: Concrete slab for SEM
}
} } -------------------------------------------------------------------
} } ----- The Microscopy ListServer -- Sponsor: The Microscopy Society
} } of America To Subscribe/Unsubscribe -- Send Email to
} } ListServer-at-MSA.Microscopy.ComOn-Line Help
} } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html--------
} } ---------------------------------------------------------------.
} }
} }
} } Steve,
} }
} } David is right.
} } Our testing has shown that saw cutting the floor to isolate the
} } column from the rest of the building has no affect other than to
} } cost a lot. The vibration is transmitted through the ground, not the
} } bldg.
} }
} } Craig Franklin
} } Vibration Engineering Consultants
} } www.vibeng.com
} }
} }
} } ----- Original Message -----
} } } From: David Cockayne {david.cockayne-at-materials.oxford.ac.uk}
} } To: {Microscopy-at-sparc5.microscopy.com}
} } Sent: Friday, October 13, 2000 5:16 PM
} } Subject: Re: Concrete slab for SEM
} }
} }
} } } -----------------------------------------------------------------
} } -------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of
} } America} To Subscribe/Unsubscribe -- Send Email to
} } ListServer-at-MSA.Microscopy.Com} On-Line Help
} } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html} ------
} } -----------------------------------------------------------------.
} } }
} } }
} } } Earl
} } }
} } } The optimum thickness of concrete is whatever thickness of
} } concrete it
} } } takes to stop the train. Any other answer is incorrect.
} } }
} } }
} } } David C
} } }
} } }
} } } ----- Original Message -----
} } } } From: Earl Weltmer {earlw-at-pacbell.net}
} } } To: Buckingham, Steve {sbuckingham-at-excellatron.com}
} } } Cc: 'Microscopy-at-MSA.Microscopy.Com'
} } {Microscopy-at-sparc5.microscopy.com} } Sent: Friday, October 13, 2000
} } 3:49 PM
} } } Subject: Re: Concrete slab for SEM
} } }
} } }
} } } } ---------------------------------------------------------------
} } ---------
} } } } The Microscopy ListServer -- Sponsor: The Microscopy Society
} } of America
} } } } To Subscribe/Unsubscribe -- Send Email to
} } ListServer-at-MSA.Microscopy.Com} } On-Line Help
} } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html} } ----
} } -------------------------------------------------------------------.
} } } }
} } } }
} } } } Hi Steve,
} } } }
} } } } I don't think there is an "optimum" thickness for the concrete
} } slab.} } If vibration from the railroad (or any other source) is a
} } concern most
} } } isolate
} } } } the main slab by sawing & removing the concrete under the optics
} } console.
} } } A
} } } } new concrete slab is laid with "pea sized agregate" & felt on
} } the sides.
} } } This
} } } } lets the column stand free from the rest of the slab.
} } } }
} } } } Regards,
} } } }
} } } } Earl Weltmer
} } } }
} } } } "Buckingham, Steve" wrote:
} } } }
} } } }
} } } -----------------------------------------------------------------
} } -------
} } } } } The Microscopy ListServer -- Sponsor: The Microscopy Society
} } } } } of
} } America
} } } } } To Subscribe/Unsubscribe -- Send Email to
} } ListServer-at-MSA.Microscopy.Com
} } } } } On-Line Help
} } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } }
} } } -----------------------------------------------------------------
} } ------.
} } } } }
} } } } } Hi All,
} } } } } I have a JSM 840 that I need to relocate soon. The new
} } location
} } } is
} } } } } very near a railroad and interstate! Does anyone have
} } information on
} } the
} } } } } optimum thickness of a concrete slab to prevent vibrations?
} } } } } Thanks,
} } } } }
} } } } } Steve Buckingham
} } } } } Excellatron Solid State LLC
} } } } } 1460 Roswell Street, Suite J
} } } } } Smyrna, GA 30080
} } } } } phone 770 438 2201
} } } } } fax 770 438 2118
} } } }
} } } }
} } } }
} } }
} } }
} } }
} } }
} }
} }
} }
} }
}
}



Robin H Cross
Director : EM Unit, Rhodes University, Grahamstown, South Africa
R.Cross-at-ru.ac.za
tel: +27 46 603 8168 - fax: +27 46 622 4377
http://www.ru.ac.za/emu/index.htm

** remember that ICEM-15 in 2002 is in Durban, South Africa **


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for Microscopy-at-sparc5.microscopy.com; Mon, 16 Oct 2000 08:30:22 +0100
Message-ID: {001701c03742$383d8920$c74101a3-at-materials.ox.ac.uk}


Dear Mike

I am sure that increasing the mass under the microscope works, as you say.
But the question was what is the "optimum thickness to stop vibrations".
There is only one "optimum", and I believe my answer was it.

David
----- Original Message -----
} From: {"MAOKeefe-at-lbl.gov"-at-sparc5.microscopy.com}
To: Craig Franklin {franklin-at-vibeng.com}
Cc: MSA List Server {Microscopy-at-sparc5.microscopy.com} ; DavidCockayne
{david.cockayne-at-materials.oxford.ac.uk}
Sent: Sunday, October 15, 2000 8:03 PM


Craig, David, Earl, Steve,

Increasing the mass under the microscope works. An isolated room-size
slab of 1m thickness can reduce vertical vibration by a factor of four
and horizontal vibration by 10 to 12 times [1]. This foundation was a
significant factor in allowing us the obtain a TEM resolution better
than one Angstrom [2].

-Mike O'Keefe

1. "Design and implementation of a site for a one-Ångstrom TEM", John H.
Turner, Michael A. O'Keefe and Robert Mueller, in 55th Ann. Proc. MSA,
Cleveland, Ohio (1997) 1177-1178.
2. "The NCEM One-Ångstrom Microscope Project Reaches 0.89Å Resolution",
Michael A. O'Keefe and Y.C. Wang in 58th Ann. Proc. MSA, Philadelphia,
Pennsylvania (2000).

----- Original Message -----
} From: "Craig Franklin" {franklin-at-vibeng.com}


Colleagues...

Can anyone recommend a introductory text for this person. Remember
Ask-A-Microscopist is a forum that is used by NON-MICROSCOPISTS
looking for help so keep try to your technical details at the right level.

Nestor
---------------------------------------------------


Below is the result of your feedback form. It was submitted by
(sfield-at-island.net) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Sunday, October
15, 2000 at 23:18:56
---------------------------------------------------------------------------

Email: sfield-at-island.net
Name: Shane Field

School: Hard Knocks

State: BC

Zip: V0N 3E0

Question: I recently came into possession of an old Bushnell microscope
(the kind that is kept in some high school science labs for 2 or 3
generations). On the top of the scope it says--CENCO 506-L Bushnell Triple
Tested 667157. (This may have been the 57 Chev 283 of scopes found in
biology classrooms all over North America) Anyway, I took Physics and
Chemistry in high school and never did learn how to operate a simple
microscope. Imagine my chagrin at not being able to figure out how it works
--I am able to view objects at low power but not high power. Would you be
able to reccomend a good (but simple) Coles Notes kind of booklet that will
give me the basics of lighting,focussing/eye piece selection etc? etc.?
Thank you-TY TY TY!

---------------------------------------------------------------------------





From daemon Mon Oct 16 08:55:05 2000



From: Leona Cohen-Gould :      lcgould-at-mail.med.cornell.edu
Date: Mon, 16 Oct 2000 09:48:33 -0400
Subject: Re: TEM of dynabeads

Contents Retrieved from Microscopy Listserver Archives
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At 4:03 PM -0500 10/13/00, Theresa Fassel wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Lee

Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175





From daemon Mon Oct 16 08:56:41 2000



From: Tobias Baskin :      BaskinT-at-missouri.edu
Date: Mon, 16 Oct 2000 08:50:15 -0500
Subject: Re: Microscopy request from TV Show NOVA

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greetings,
Years ago, I made 16 mm movies from a microscope as follows.
Hooked up an ordinary video camera to the scope and displayed the
image on a high quality monitor. In front of the monitor was a wooden
square box that telescoped and was black on the inside. At the end of
that was a 16 mm movie camera, focussed on the monitor. One frame of
the move was exposed in a 1/4 sec exposure so we were running much
slower than the tv raster rate. We were actually doing timelapse
studies like this and got very nice images for playback and analysis.
This was done in the lab of Ray Keller at UC Berkeley who would have
much more of the details to hand if you want to follow this route up.

Could this help??
Tobias Baskin


} The following is a request from a producer of the PBS TV show NOVA, which
} came in to the MSA Business office.
}
} Can anyone help? Please correspond directly to the requester.
}
} } } } } } }
}
} Hi Folks --
}
} I'm a producer with the science TV series NOVA. I'm working on a show
} about evolution and I'm desperately looking for a way to adapt a zeiss
} axiophot microscope to an Aaton 16mm film camera. So far I've struck
} out on finding anyone who has gear to accomplish this feat.
}
} Any suggestiongs?
}
} --
} Chris Schmidt
} Powderhouse Productions
} 259 Elm Street
} Somerville MA 02144
} 617-629-2200 work
} 617-776-7905 fax
} 781-820-0727 mobile
} 617-403-8699 pager
} 781-646-0928 home
} Chris-at-powderhouse.net

--
_ ____ __ ____ Tobias I. Baskin
/ \ / / \ / \ \ 109 Tucker Hall
/ / / / \ \ \ Biological Sciences
/_ / __ /__ \ \ \__ University of Missouri
/ / / \ \ \ Columbia, MO USA
/ / / \ \ \ 65211-7400
/ / ___ / \ \__/ \ ____ voice: 573-882-0173
fax: 573-882-0123



From daemon Mon Oct 16 12:21:09 2000



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Mon, 16 Oct 2000 11:39:59 -0500
Subject: Re: Microscopy request from TV Show NOVA

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Another poster related his success at using an intermediate monitor for
getting the image onto 16 mm film. I might take that a step further.

I don't know film production techniques, but was of the impression that
much of the editing has gone digital. If that be the case, are there not
utilities to record high resolution digital movies from microscope cameras.
I thought Sony had a rather decent digital video camera that John McKenzie
was touting a year or two ago. Even better cameras should be available now.

Please remember the final use and display of the sequence. I know that film
can provide greater detail than digital for still photographs, but I doubt
that this sequence would be enlarged to the degree that resolution would be
an issue. Then there is the question of final display. If I viewed the
final result on my plain, old TV, most any camera system would provide more
resolution than I could appreciate. If it was viewed on an HDTV, more
detail would be visible, but still not as much as a good video camera
should be able to provide. Maybe an IMAX presentation would require all the
resolution a camera could provide and then some. But by then, I would guess
the resolution of the microscope would be more of a limitation than the
resolution of the camera.

In short, I would suggest collecting and processing the sequence in digital
format until the final stage and then transferring it to 16-mm if need be.

Warren Straszheim

At 01:04 PM 10/15/2000 -0400, you wrote:

} The following is a request from a producer of the PBS TV show NOVA, which
} came in to the MSA Business office.
}
} Can anyone help? Please correspond directly to the requester.
} } } } } } }
} Hi Folks --
}
} I'm a producer with the science TV series NOVA. I'm working on a show
} about evolution and I'm desperately looking for a way to adapt a zeiss
} axiophot microscope to an Aaton 16mm film camera. So far I've struck
} out on finding anyone who has gear to accomplish this feat.
}
} Any suggestiongs?
}
} --
} Chris Schmidt
} Powderhouse Productions
} 259 Elm Street
} Somerville MA 02144
} 617-629-2200 work
} 617-776-7905 fax
} 781-820-0727 mobile
} 617-403-8699 pager
} 781-646-0928 home

----------------------
Warren E. Straszheim
Materials Analysis and Research Lab
Iowa State University
23 Town Engineering
Ames IA, 50011-3232

Ph: 515-294-8187
FAX: 515-294-4563

E-Mail: wesaia-at-iastate.edu
Web: www.marl.iastate.edu

Scanning electron microscopy, x-ray analysis, and image analysis of materials
Computer applications and networking



From daemon Mon Oct 16 14:18:30 2000



From: George Langford :      amenex-at-amenex.com
Date: Mon, 16 Oct 2000 15:07:43 -0400
Subject: Rescuing outdated Polapan CT 35mm film

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hallo Microscopists !

After attempting a test roll of Polapan CT 35mm instant film, I
discovered what "outdated" means in the Polaroid context - the
negative failed to strip off the celluloid and the strip looked
unusable.

Later, after finding that there are no known dealers of this
film in my area (SE PA) I retrieved the film from the waste
basket & washed it in warn water, whereupon, to my utter
amazement, usable positive images appeared out of the murk.

I am now waiting for the film strip to dry, after dipping it
in PhotoFlo as I am used to doing with Type 55 P/N negatives.

Is there a more conservative way to strip the negative off
the film ? My method seems rather crude ...

Best regards,
George Langford
amenex-at-amenex.com



From daemon Mon Oct 16 16:22:05 2000



From: Gordon Couger :      gcouger-at-couger.com
Date: Mon, 16 Oct 2000 16:13:52 -0500
Subject: Re: Concrete slab for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} Craig, David, Earl, Steve,
}
} Increasing the mass under the microscope works. An isolated room-size
} slab of 1m thickness can reduce vertical vibration by a factor of four
} and horizontal vibration by 10 to 12 times [1]. This foundation was a
} significant factor in allowing us the obtain a TEM resolution better
} than one Angstrom [2].
}
} -Mike O'Keefe
}
} 1. "Design and implementation of a site for a one-Ångstrom TEM", John
} H. Turner, Michael A. O'Keefe and Robert Mueller, in 55th Ann. Proc.
} MSA, Cleveland, Ohio (1997) 1177-1178. 2. "The NCEM One-Ångstrom
} Microscope Project Reaches 0.89Å Resolution", Michael A. O'Keefe and
} Y.C. Wang in 58th Ann. Proc. MSA, Philadelphia, Pennsylvania (2000).
}
The concrete slab need to sit on dry sand to minimize coupling to the
soil/rock below it. A layer of gravel with drain tile laid in it covered
by a 2 or 3 foot layer of sand would be a starting point. If the drain
tile won't drain by gravity a sump will be needed to pump out any water. A
water barrier need to be placed below the gravel. The truly paranoid would
put down 6 inches of tamped sand, 4 inches of benotnite clay, a layer of
heavy plastic enough sand to keep the installation of gravel from
puncturing the plastic. The benonite serves as a self sealing moisture
barrier. It is a clay that swells when it gets wet. It is not particularly
expensive if you buy it from a oil field mud company. They buy it in train
car loads and sell it in truck loads or sacks.

Gordon
Gordon Couger gcouger-at-couger.com
Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00





From daemon Mon Oct 16 18:23:47 2000



From: Caroline Schooley :      schooley-at-mcn.org
Date: Mon, 16 Oct 2000 16:14:21 -0700
Subject: Re: Ask-A-Microscopist: Recommendation needed booklet that will

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} Question: I recently came into possession of an old Bushnell microscope
} (the kind that is kept in some high school science labs for 2 or 3
} generations). On the top of the scope it says--CENCO 506-L Bushnell Triple
} Tested 667157. (This may have been the 57 Chev 283 of scopes found in
} biology classrooms all over North America) Anyway, I took Physics and
} Chemistry in high school and never did learn how to operate a simple
} microscope. Imagine my chagrin at not being able to figure out how it works
} --I am able to view objects at low power but not high power. Would you be
} able to reccomend a good (but simple) Coles Notes kind of booklet that will
} give me the basics of lighting,focussing/eye piece selection etc? etc.?
} Thank you-TY TY TY!
}
Shane -

I hope that you'll be willing to go a step beyond "booklet". There's a
very good basic book; here's the review from the Project MICRO bibliography
(URL below):

Nachtigall, W. 1995 Exploring With the Microscope 160 pp. hardbound.
6.5x9.5". $19.95. ISBN 0-8069-0866-1 Sterling Publishing Co., NY.
Although this book is intended for adult amateur microscopists, it
is well written and will provide teachers and classroom volunteers with
much useful information on "serious" light microscopy; it's unequalled as a
basic reference for beginners. Almost half of the book is devoted to
simple preparation methods for biological specimens and descriptions (with
good illustrations) of commonly encountered organisms. Adult. RECOMMENDED

If that's beyond your butget, shop around the MICRO bibliography. You may
find enough basic info in the website hotlink list.

Caroline

Caroline


Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html





From daemon Mon Oct 16 21:37:19 2000



From: Ronald Austin :      rla-at-mindspring.com
Date: Mon, 16 Oct 2000 21:30:44 -0500
Subject: EM tissue processor

Contents Retrieved from Microscopy Listserver Archives
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Hi George,
Well, George if you have talked to Rick and have been to a workshop I don't
know if I have any solutions for you, however, you said you were mostly
processing yeast and Drosphila Fly eyes. In my earlier day in EM I work for
a very short time on plant tissue and I found that I had to use a vacuum
device of some kind to get the resin to penetrate the tissue. Pella has a
Vacuum device you can use in the model of oven you have (which by the way is
the same as mine).
I found that nerve tissue take a little bit more fixation (3% Glut. in 0.1M
Cacodylate buffer pH 7.3) in the oven then other tissue does. The nerve I
mostly work with is sural nerve and I find that a temp setting of 37C with
an irritation time of 40sec x3. Cooling the tissue down, in wet ice, to
about 7-8C before and between each irritation period works for me.
What sort of fixative do you use with these tissues?

I have a vacuum system for that microwave here at Shreveport which is only a
3 or 4 hour trip from Dallas. Maybe you could work sometime in to come over,
bring your specimens with you.


Ron Austin (Research Associate)
LSU Medical Ct.
Dept. of Pathology
318-675-4557
Shreveport, LA
e-mail:
rla-at-mindspring.com




From daemon Tue Oct 17 02:41:21 2000



From: Claudia Hayward-Costa :      LS_S562-at-crystal.kingston.ac.uk
Date: Tue, 17 Oct 2000 08:35:26 +0100
Subject: TEM: Request for a rubber roll membrane

Contents Retrieved from Microscopy Listserver Archives
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Dear Electron Microscopists,

Could you please help us - please send replies to my email
address:

c.hayward-at-kingston.ac.uk


Hi,
We have a 30 year old Philips T301 transmission electron
microscope, and the rubber roll membrane (original part number
5322 360 40071) in the pneumatic film lifting mechanism of the
camera has split. In view of the age of the instrument it looks as if
we would only be able to replace it if some nice person out there
has a spare one that they might be prepared to part company with.
Is there anybody out there who has a spare camera lift roll
membrane please?

Thanks,

Bill Edwards.

Dr. C. Hayward-Costa
School of Life Sciences
Kingston University
Penrhyn Road, Kingston upon Thames
Surrey KT1 2EE, UK
44(0)208 547 2000 x 2240
Email: c.hayward-at-kingston.ac.uk



From daemon Tue Oct 17 04:31:46 2000



From: Paula Allan-Wojtas :      AllanWojtasP-at-em.agr.ca
Date: Tue, 17 Oct 2000 10:06:25 -0400
Subject: Food Structure and Functionality Symposium 2001

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The principle of mounting the microscope on a massive slab is
good, but I suspect that to isolate your microscope from the very low
frequency, long-wavelength vibrations caused by rail traffic you will
need a much softer and compliant isolation than can be provided by
any depth of sand or compacted aggregate. I have recently built a
garage / workshop within 50 metres of the London - Edinburgh
track. The building is on a heavy reinforced concrete raft
foundation. The slab is on 12" of sand over 3 feet of 2 to 3"
aggregate and the substrate is several metres of heavy gravel
material described by the geologists as raised beach. Nevertheless
you can feel the vibration caused by passing trains through your
feet.

My guess is that you need a fully floating slab, either mounted on
very compressible pads of rubber or similar elastomer, or raised on
an air cushion.

Vibration is a problem which many people worry about as demands
for high resolution and traffic increase, and solutions to it are
expensive. It strikes me that it would be a good move to do some
modelling of the physics of the situation before committing money to
it. Does anyone know how to go about this? Does software exist?

Chris



} From: "Gordon Couger" {gcouger-at-couger.com}
To: {R.Cross-at-ru.ac.za} , {"MAOKeefe-at-lbl.gov"-at-sparc5.microscopy.com}
Copies to: {Microscopy-at-sparc5.microscopy.com}


Food Structure & Functionality Symposium 2001 -
An international symposium leading Food Structure & Functionality studies through the 21st century


Being held in conjunction with the 92nd AOCS Annual Meeting and Expo , May 13-16, 2001, Minneapolis Convention Center, Minneapolis, Minnesota, USA. For information, e-mail us at meetings-at-AOCS.org

The mandate of the Food Structure & Functionality Forum : *To promote global collaboration between Food and Agriculture professionals in Structure and Functionality disciplines by facilitating and providing a forum for exchange of knowledge, expertise and research findings*.
The symposium has two themes:
* new and novel approaches (including microscopy, rheology and spectroscopy) to the study of structure-function relationships in foods;
* food system studies covering any part of the processing chain - from the raw material to the final product, and including trouble shooting.
preliminary program
May 13th - Short Courses (each short course will take a full day, they will be run in parallel)
1) Food Contaminants. Contact person - Mark Auty
2) Specific Labelling in Foods. Contact person - Marcel Paques

May 15th-17th inclusive - Technical sessions (6 sessions will run over 3 days)

May 14th
Morning.
Session 1. Dairy applications and fat based foods (Chairs: Mark Auty and Tony McKenna)

Afternoon
Session 2. Food Safety (Chairs: Judy Arnold and Maria Brandl )

Evening
-Round Table Discussion (RTD)
-Social program for Division members and FS&F symposium participants

May 15th
Morning
Session 3. New Methods and Techniques for Food Structure and Functionality Analysis
Chair: Kathy Groves

Afternoon
Session 4. Agricultural Applications of Microscopy and Imaging (Chairs: Delilah Wood and Paula Allan-Wojtas)

Evening
FS&F Division member meeting

May 16th
Morning
Session 5. Ingredients and Food Processing (Chairs: Diana Kittleson and Jim Charbonneau)

Afternoon
6. Colloidal and Interfacial Sciences (Chairs: Marcel Paques and David Pechak)

Poster Session
Posters will be displayed during the meeting and presented during breaks between technical sessions

Contact information for the chairs is shown below, in alphabetical order:


Paula Allan-Wojtas
Agriculture and Agri-Food Canada
Atlantic Food and Horticulture Research Centre
Kentville, Noval Scotia Canada B4N 1J5
Tel: (902) 679-5566
FAX: (902) 679-2311
email: allanwojtasp-at-em.agr.ca

Judy Arnold
USDA -ARS - RRC
950 College Station Rd.
P.O. Box 5677
Athens, GA 30604-5677
USA
Tel: (706) 546-3515
FAX: (706) 546-3068
email: jarnold-at-ars.usda.gov

Mark Auty
Dairy Products Research Centre
TEAGASC
Moorepark, Femoy, Co. Cork
Ireland
Tel: 011-353-25-42447
FAX: 011-353-25-42340
email: mauty-at-moorepark.teagasc.ie

Maria Brandl
USDA - ARS - WWRC
800 Buchanan Street
Albany, CA 94710
USA
Tel: (510) 559-5885
FAX: (510) 559-5948
email:mbrandl-at-pw.usda.gov

James E. Charbonneau
National Food Processors Association
Food Chemistry and Packaging Department
1401 New York Ave, NW
Washington, D.C. 20005
USA
Tel: (202) 639-5972
FAX: (202) 639-5991
email: jcharbo-at-nfpa-food.org

Kathy Groves
Leatherhead Food Research Association
Randalls Road, leatherhead
Surrey KT22 7RY
England
Tel: 44 0132 822330
FAX: 44 0132 386228
email: kgroves-at-lfra.co.uk


Diana Kittleson
Pillsbury TPC Labs
737 Pelham Blvd.
St. Paul, MN 55114
USA
Tel: (651) 917-5859
FAX: (651) 917 5850
email: dkittleson-at-pillsbury.com

Tony McKenna
New Zealand Dairy Institute
Private Bag 11 029
Palmerston North,
New Zealand
Tel: 011 64 6 350 4649
FAX: 011 64 6 356 1476
email: tony.mckenna-at-nzdri.org.nz

Marcel Paques
Wangeningen Centre for Food Sciences/Unilever Research Vlaardingen
P.O. Box 20, 6710 BA Ede
The Netherlands
Tel: 011 31 318 659690
FAX: 011-31-318 650400
email: paques-at-nizo.nl

David Pechak
Kraft Technology Centre
801 Waukegan Road
Glenview, IL 60025
USA
Tel: (847) 646-4808
FAX: (847) 646-3864
email: dpechak-at-kraft.com

Delilah Wood
USDA - ARS - WWRC
800 Buchanan Street
Albany, CA 94710
USA
Tel: (510) 559-5653
FAX: (510) 559-5777
email: wood-at-pw.usda.gov



From daemon Tue Oct 17 10:32:04 2000



From: Geoff Williams :      willi1gl-at-cmich.edu
Date: Tue, 17 Oct 2000 11:28:17 -0400
Subject: Re: Concrete slab for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


All this talk of huge amounts of concrete and agregate and sand. . .

What about isolation platforms? While it might be slightly more
expensive - at least you
won't be left with a huge construction bill if your concrete slab
doesn't isolate enough
vibration.

TMC makes specific floor platforms. We were looking at their tables
when ordering a
Confocal Mircosope. And from the catalog and price list you can get a
platform that will
easily isolate the column from nearly all vibration for around $5000 to
$6000 dollars. It
looks like they also make active vibration cancelling systems too.

Simple things like elastomer and rubber bases for concrete slabs have a
fatigue life,
which could be shorter than the remain life of the microscope.

I have no finacial interest in TMC: http://www.techmfg.com

I cannot see how you will isolate train vibrations without a system a
little more complex
than a concrete slab.


Geoff Williams

Electron Microscope Facility Supervisor
Biology Department
Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859

Geoffrey.Lloyd.Williams-at-cmich.edu
517 774-3576
517 774-3462 (fax)




From daemon Tue Oct 17 12:18:40 2000



From: Phillip Rutledge :      prutledge-at-ars.usda.gov
Date: Tue, 17 Oct 2000 11:13:23 -0600
Subject: Re: Concrete slab for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} } } "Buckingham, Steve" {sbuckingham-at-excellatron.com} 10/12/00 18:55 PM } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Hi All,
I have a JSM 840 that I need to relocate soon. The new location is
very near a railroad and interstate! Does anyone have information on the
optimum thickness of a concrete slab to prevent vibrations?
Thanks,

Steve Buckingham
Excellatron Solid State LLC
1460 Roswell Street, Suite J
Smyrna, GA 30080
phone 770 438 2201
fax 770 438 2118

Steve,

Have you tried something cheaper? When I was with The Johns Hopkins University School of Medicine we were remodeling the lab. A new darkroom sink for developing negatives was being put in right beside the TEM. The plumbers had to drill for water lines and a sink drain within 3-5 feet of the microscope. We lifted up each corner of the microscope and installed 60 or 80 durometer(I think it was 80), 4"x4"x1" rubber pads and could use the microscope without vibration. The lab was located just below street level. This might be a way to go before trying concrete slabs. The only way I've heard of using concrete slabs was to isolate the slab from the rest of the floor. A concrete slab could be very expensive. Most rubber supply houses can supply the pads. Hope you can work it out.

Phil Rutledge
USDA/ARS
151 Dixon Drive
Chestertown, MD 21620
voice: 410.778.4136 or 2120
fax: 410.778.4399
e-mail: rutledge-at-ars.usda.gov






From daemon Tue Oct 17 14:14:33 2000



From: Greg Erdos :      gwe-at-biotech.ufl.edu
Date: Tue, 17 Oct 2000 15:13:01 -0400
Subject: MSA Videotapes available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Videotapes from M&M 2000 are now available for purchase. A new list of
all tapes in the collection can be found at the MSA web site or directly at
: http://www.biotech.ufl.edu/sems/newtape00.htm

Many of the older tapes are available for immediate shipment. Others,
including the 2000 tapes, must be ordered from the video duplication
service. This usually requires about two weeks. Please remember to order
the tapes by number and title. Make checks payable to "MSA" in US funds
only. Purchase orders can be accepted. Orders may be placed by phone,
mail, fax or e-mail to the contact at the bottom of this message.

If you are a presenter in any of the tapes and feel that your tape should
not be offered for sale in the future for whatever reason (obsolete
information, bad hair day, etc.) please let me know.

Greg Erdos
Videotape Wrangler
Gregory W. Erdos, Ph.D.
Assistant Director, Biotechnology Program
PO Box 110580
University of Florida
Gainesville, FL 32611

Ph. 352-392-1295 Fax: 352-846-0251



From daemon Tue Oct 17 14:21:16 2000



From: crowj-at-mail.leon.k12.fl.us ()
Date: Tue, 17 Oct 2000 14:19:11 -0500
Subject: Ask-A-Microscopist: bacteria and fungi and yeast

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Colleagues...

Can anyone answer this one?.
Remember Ask-A-Microscopist is a forum that is used by NON-MICROSCOPISTS
and K-12 Students looking for help so keep try to your technical details at
the right level.

Nestor
---------------------------------------------------
Below is the result of your feedback form. It was submitted by
(crowj-at-mail.leon.k12.fl.us) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Tuesday, October
17, 2000 at 11:33:21
---------------------------------------------------------------------------

Email: crowj-at-mail.leon.k12.fl.us
Name: students

School: The Academic Resource Center

State: Florida

Zip: 32304

Question: Students are working with bacteria and fungi and yeast. They
want to know why yeast is killed at temperatures lower than body
temperatures, yet bacteria can grow on human bodies with a much higher
temperature (98.2 according to most sources is the average body temp).

---------------------------------------------------------------------------





From daemon Tue Oct 17 15:45:18 2000



From: Robert Palmer :      rjpalmer-at-dir.nidcr.nih.gov
Date: Tue, 17 Oct 2000 16:45:50 -0500
Subject: M&M proceedings

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello List -
Have I waited long enough to receive my proceedings volumes from the
Philadelphia M&M meeting? If so, whom should I contact regarding
"re-shipment"?
Robert J. Palmer Jr., Ph.D.
Natl Inst Dental Craniofacial Res - Natl Insts Health
Oral Infection and Immunity Branch
Bldg 30, Room 308
30 Convent Drive
Bethesda MD 20892
ph 301-594-0025
fax 301-402-0396



From daemon Tue Oct 17 16:28:32 2000



From: Amy McGough :      amcgough-at-bilbo.bio.purdue.edu
Date: Tue, 17 Oct 2000 16:33:17 -0500
Subject: TEM positions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Dear Colleagues,

We have both technical and postdoctoral openings for transmission
electron microscopists interested in studying biological
macromolecules and cellular assemblies to high resolution using
electron cryomicroscopy. The recent research emphasis of our
laboratory has been on the molecular mechanisms underlying the
assembly, organization, and dynamics of the cytoskeleton. Stable
funding is available for these positions for 5 years. Salaries are
negotiable.

The Department of Biological Sciences provides outstanding modern TEM
facilities including Philips CM300-FEG, CM200-FEG, and EM420 electron
cryo-microscopes dedicated to structural studies. Both the 300kV and
200kV field emission gun microscopes are outfitted with CCD cameras,
2k x 2k and 1k x 1k respectively, in addition to film cameras.

Purdue is home to over 50,000 students, faculty, and staff, and is
located in West Lafayette, Indiana - approximately 1 hour northwest
of Indianapolis and 2.25 hours southeast of Chicago. The area affords
the advantages associated with a major university, while providing an
affordable and attractive 'small town' environment in which to live.

The ideal candidates will be enthusiastic, self-motivated individuals
with strong communication skills who are committed to applying an
integrated structure-function approach to the study of biological
macromolecules. Interested individuals should contact Dr. McGough by
e-mail or phone. Formal applications for the technical positions
should be made via the Personnel Services department
http://www.adpc.purdue.edu/Personnel/job-home.htm.

Purdue is an equal access/equal opportunity university.



Amy M. McGough, Ph.D.
Assistant Professor, Department of Biological Sciences
and Editorial Board Member, FEBS Letters
Purdue University
West Lafayette, IN 47907-1392
E-mail: amcgough-at-bilbo.bio.purdue.edu
Office: 765-496-7501; Lab: 765-496-7716
Secretary: 765-494-4954; Fax: 765-496-1189
CM200F: 765-496-2746; CM300F: 765-496-7508
http://bilbo.bio.purdue.edu/~amcgough/
Biophysics & Cell Biology Symposium: http://bilbo.bio.purdue.edu/~rbernal/



From daemon Tue Oct 17 16:29:56 2000



From: Ekstrom, Harry :      harry.ekstrom-at-honeywell.com
Date: Tue, 17 Oct 2000 14:27:58 -0700
Subject: Concrete slab for SEM

Contents Retrieved from Microscopy Listserver Archives
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Aside from all the concrete, sand and aggregate issues, the column on our
Cameca microprobe rests on inflatable "feet" that helps reduce vibrations as
well. This of course is in addition to an isolation slab that it sits on
that was poured first.

Harry Ekstrom
Materials Laboratory

(602) 231-2744
e-mail: harry.ekstrom-at-honeywell.com


-----Original Message-----
} From: Buckingham, Steve [mailto:sbuckingham-at-excellatron.com]
Sent: Thursday, October 12, 2000 10:59 AM
To: 'Microscopy-at-MSA.Microscopy.Com'


Hi All,
I have a JSM 840 that I need to relocate soon. The new location is
very near a railroad and interstate! Does anyone have information on the
optimum thickness of a concrete slab to prevent vibrations?
Thanks,

Steve Buckingham
Excellatron Solid State LLC
1460 Roswell Street, Suite J
Smyrna, GA 30080
phone 770 438 2201
fax 770 438 2118






From daemon Tue Oct 17 19:24:25 2000



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Wed, 18 Oct 2000 13:23:50 GMT+1200
Subject: Rubber Pads for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Maybe the rubber pads might help a bit, but is there actually going
to be a problem?
The 840 has really good spring suspension anyway, I can't see that
rubber pads would add significant isolation to what's there already.

Might be instructive to ask if anyone's actually experienced
vibration sensitivity with an 840.

rtch



}
}
} Hi All,
} I have a JSM 840 that I need to relocate soon. The new location is
} very near a railroad and interstate! Does anyone have information on
} the optimum thickness of a concrete slab to prevent vibrations?
} Thanks,
}
} Steve Buckingham
} Excellatron Solid State LLC
} 1460 Roswell Street, Suite J
} Smyrna, GA 30080
} phone 770 438 2201
} fax 770 438 2118
}
} Steve,
}
} Have you tried something cheaper? When I was with The Johns Hopkins
} University School of Medicine we were remodeling the lab. A new
} darkroom sink for developing negatives was being put in right beside
} the TEM. The plumbers had to drill for water lines and a sink drain
} within 3-5 feet of the microscope. We lifted up each corner of the
} microscope and installed 60 or 80 durometer(I think it was 80),
} 4"x4"x1" rubber pads and could use the microscope without vibration.
} The lab was located just below street

Phil Rutledge USDA/ARS 151
} Dixon Drive Chestertown, MD 21620 voice: 410.778.4136 or 2120 fax:
} 410.778.4399 e-mail: rutledge-at-ars.usda.gov
}
}
}
}
Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand



From daemon Tue Oct 17 19:25:45 2000



From: Jennifer E. Taylor :      jtaylor-at-stevens-tech.edu
Date: Tue, 17 Oct 2000 20:23:05 -0400
Subject: PEG sample prep.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear List Members,
}
} We are trying to section by microtome samples of solution-cast PEG.
PEG
} is dissolved in water and THF and allowed to rest until it becomes a
} viscous solution by the evaporation of the solvent. It is then put
into
} molds and under vacuum to further rid the material of solvent.
Finally,
} the PEG becomes solid.
}
} THE PROBLEM: The PEG moldings can not be sectioned because they are
too
} brittle and crumble in the chuck.
}
} Does anyone have a protocol for preparing PEG samples that does not
lead
} to this end?
}
} Thank you very much,
}
} Jennifer Taylor




From daemon Wed Oct 18 03:47:59 2000



From: Stephan Helfer :      S.Helfer-at-rbge.org.uk
Date: Wed, 18 Oct 2000 09:42:05 BST
Subject: Re: Ask-A-Microscopist: bacteria and fungi and yeast

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear students

Thanks for your interest. There are around 500-600 different species
of yeast, many of them unrelated genetically. Some of these, such as
some species of Sporobolomyces have temperature requirements that are
below normal body heat (37C). Others, such as Candida albicans, are
pathogenic on humans, with their optimum temperature at normal body
heat. The bakers' yeast, Saccharomyces cerevisiae, can survive 40C
for short periods, but has its growth optimum at around 27C. There
are specially selected strains (especially used in brewing) which
have other growth optima.

Whilst I do not know the details, I believe that bacteria have
similarly varied optimal growth temperatures (e.g. the iron bacterium
Gallionella at 20C, the yoghurt bacterium Streptococcus thermophilus at
over 45C).
I hope this helps.

Cheers

Stephan Helfer PhD
Mycologist, Royal Botanic Garden Edinburgh

} Email: crowj-at-mail.leon.k12.fl.us
} Name: students
}
} School: The Academic Resource Center
}
} State: Florida
}
} Zip: 32304
}
} Question: Students are working with bacteria and fungi and yeast. They
} want to know why yeast is killed at temperatures lower than body
} temperatures, yet bacteria can grow on human bodies with a much higher
} temperature (98.2 according to most sources is the average body temp).
}
} ---------------------------------------------------------------------------
}
}
}
}

Cheers

Stephan
0131 248 2865
FAX 248 2901
http://www.rbge.org.uk



From daemon Wed Oct 18 04:05:25 2000



From: Jim.Slater-at-mcmail.vanderbilt.edu
Date: Tue, 17 Oct 2000 18:53:27 -0500
Subject: H-600

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The Department of Cell Biology -at- Vanderbilt University has a Hitachi H-600
TEM which we would like to disassemble and remove.

If interested in obtaining this equipment item contact Jim Slater by
October 26, 2000 -at- jim.slater-at-mcmail.vanderbilt.edu or (615) 343-4905.

Jim Slater
Administrative Officer
Department of Cell Biology
Vanderbilt University School of Medicine
Nashville, TN 37232-2175






From daemon Wed Oct 18 06:55:45 2000



From: Larry Allard :      L2A-at-ornl.gov
Date: Wed, 18 Oct 2000 07:53:05 -0400
Subject: Re: M&M proceedings

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Robert:

you may know this already, but...

I called on Monday regarding the missing Proceedings, and found that
there were 3 mailing lists, and they are now working on the third
list, so it could be an additional 2 weeks before the final volumes
are received. Hang in there...

Larry





} \------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Dr. Lawrence F. Allard
Senior Research Staff Member
High Temperature Materials Laboratory
Oak Ridge National Laboratory
1 Bethel Valley Road
Bldg. 4515, MS 6064
PO Box 2008
Oak Ridge, TN 37831-6064

865-574-4981
865-576-5413 Fax
allardlfjr-at-ornl.gov



From daemon Wed Oct 18 07:54:10 2000



From: Joseph Neilly :      joe.p.neilly-at-abbott.com
Date: Wed, 18 Oct 2000 07:52:48 -0500
Subject: PEG sample prep.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Jennifer,

PEG comes in different molecular weights. You might try a lower molecular
weight PEG whcih would be less crumbly.

Also, John Wolosewick developed a technique to section biological tissue
embedded in PEG. When I worked in his lab, we used to attach the PEG
embedded tissue to aluminum stubs with dental wax and put the stub in the
chuck of the microtome. That would prevent the chuck of the microtome from
crushing your sample.

Joe Neilly
Abbott Laboratories
D-45M, AP31
200 Abbott Park Rd.
Abbott Park, IL 60064-6202
voice: (847)-938-5024
fax: (847)-938-5027
e-mail: joe.neilly-at-abbott.com






jtaylor-at-stevens-tech.edu on 10/17/2000 09:21:39 PM
Please respond to jtaylor-at-stevens-tech.edu
To: Microscopy-at-sparc5.Microscopy.Com
cc:


Dear List Members,
}
} We are trying to section by microtome samples of solution-cast PEG.
PEG
} is dissolved in water and THF and allowed to rest until it becomes a
} viscous solution by the evaporation of the solvent. It is then put
into
} molds and under vacuum to further rid the material of solvent.
Finally,
} the PEG becomes solid.
}
} THE PROBLEM: The PEG moldings can not be sectioned because they are
too
} brittle and crumble in the chuck.
}
} Does anyone have a protocol for preparing PEG samples that does not
lead
} to this end?
}
} Thank you very much,
}
} Jennifer Taylor








From daemon Wed Oct 18 08:20:08 2000



From: Leona Cohen-Gould :      lcgould-at-mail.med.cornell.edu
Date: Wed, 18 Oct 2000 09:17:12 -0400
Subject: Re: PEG sample prep.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 8:23 PM -0400 10/17/00, Jennifer E. Taylor wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

The problem may be the molcular weight of PEG that you are using. Years
ago, I used PEG to embed heart muscle for TEM, and if memory serves, we
used a mixture of MW 10,000 and 6,000 to obtain a "sectionable"
consistancy. Our source of information at that time was work by John
Wolosowick (this was in the early-to-mid 1980's). A similar mixture may
work for you. I'lm sorry I can't be more speciic, but that was work done
at another institution, for a PI who has since passed away, so I don't have
immediate access to the records.
Try looking up John's work (It was Wolosowick & Keith Porter, I believe).

Good luck,
Lee

Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175





From daemon Wed Oct 18 08:48:47 2000



From: Jennifer Jackson :      JJackson-at-nu.ac.za
Date: Wed, 18 Oct 2000 08:45:45 -0500
Subject: BSA-gold uptake

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi

I'm planning to BSA-gold uptake into human cell-culture for TEM. Cell lines
are grown in DMEM-F12. With the aim to study the ultrastructure of the
endocytic system and determine the pH of the various organelles. Many
methods state that they grew the cells overnight in serum-free media before
starting the uptake. Does anybody know if this alters the morphology and/or
pH of the cell?

Jennifer Jackson
University of Natal, Pietermaritzburg
South Africa
email: jacksonj-at-nu.ac.za.





From daemon Wed Oct 18 09:59:50 2000



From: Ford M. Royer :      froyer-at-bitstream.net
Date: Wed, 18 Oct 2000 09:50:04 -0500
Subject: Ultra Microtome Available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have the following ultramicrotome available for sale.

Reichert (Leica) "Supernova" WITH Vibration Table ...$3,500.00

Excellent condition. Sold "As-Is, Where-Is, in Working Condition".

Please contact me directly if you are interested.

Thanks,

~ Ford
--
Ford M. Royer, MT(ASCP)
Analytical Instruments, Ltd
(Refurbished Histology, Cytology, & General Lab Equipment)
9921 13th Ave. N.
Minneapolis, MN 55441-5004
phone: 800-565-1895, Ext. 17
fax: 612-929-1895
Email: froyer-at-bitstream.net
web site: http://www.aibltd.com





From daemon Wed Oct 18 10:04:59 2000



From: Greg Strout :      gstrout-at-ou.edu
Date: Wed, 18 Oct 2000 10:00:24 -0500
Subject: Re: PEG sample prep.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


When I did some PEG work, a piece of the PEG embedded sample was glued to a
blank epoxy block and then sectioned. There are several papers out there on
the technique.

Polyethylene Glycol (PEG) Embedding and Subsequent De-embedding as a Method
for the Structural and Immunocytochemical Examination of Biological
Specimens by Electron Microscopy. Histake Kondo. 1984. J. Electr. Micros.
Tech., 1:227-241.

The application of polyethylene glycol (PEG) to electron microscopy. J.J.
Wolosewick. 1980. J. Cell Biol., 86:675-681.

Greg

"Jennifer E. Taylor" wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Dear List Members,
} }
} } We are trying to section by microtome samples of solution-cast PEG.
} PEG
} } is dissolved in water and THF and allowed to rest until it becomes a
} } viscous solution by the evaporation of the solvent. It is then put
} into
} } molds and under vacuum to further rid the material of solvent.
} Finally,
} } the PEG becomes solid.
} }
} } THE PROBLEM: The PEG moldings can not be sectioned because they are
} too
} } brittle and crumble in the chuck.
} }
} } Does anyone have a protocol for preparing PEG samples that does not
} lead
} } to this end?
} }
} } Thank you very much,
} }
} } Jennifer Taylor

--
==================================================================
Greg Strout
Electron Microscopist, University of Oklahoma
WWW Virtual Library for Microscopy:
http://www.ou.edu/research/electron/www-vl/
e-mail: gstrout-at-ou.edu
Opinions expressed herein are mine and not necessarily those of
the University of Oklahoma
==================================================================





From daemon Wed Oct 18 10:15:19 2000



From: Dee Breger :      micro-at-ldeo.columbia.edu
Date: Wed, 18 Oct 2000 11:11:08 -0400 (EDT)
Subject: digital info replies

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Listers,

On Oct 10 I posted a request for digital imaging info on behalf of graphics
prof Jonathan Lipkin (jlipkin-at-ramapo.edu). I've seen a reply from Warren
Straszheim (thanks, Warren!) but I've discovered that in that time period I
didn't receive an unknown quantity of incoming email (including my own
posting) and have no idea if anyone else responded also. So if any of you
posted a reply to Jonathan and assumed I'd been able to forward it to him,
I'd appreciate it if you'd send it to me again privately.

Many thanks,
Dee


***************************************************************
Dee Breger
Mgr. SEM/EDX Facility
Lamont-Doherty Earth Observatory
61 Route 9W
Palisades, NY 10964 USA
T: 914/365-8640
F: 914/365-8155

http://www.ldeo.columbia.edu/micro
http://www.discovery.com/area/science/micro/micro1.html
http://www.lsc.org/antarctica/front.html
Journeys in Microspace (Columbia University Press, 1995)





From daemon Wed Oct 18 10:49:16 2000



From: Lehman, Ann :      Ann.Lehman-at-trincoll.edu
Date: Wed, 18 Oct 2000 11:44:51 -0400
Subject: available print processors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have 3 print processors looking for homes - 2 free, one (new) for some
fee.

Check out details at MSA's surplus equipment site:
http://www.msa.microscopy.com/SurplusEquipment/SurplusListings.html


Ann Hein Lehman
EM Facility Manager
Trinity College
Hartford, CT 06106
v. 860-297-4289
f. 860-297-2538
e. ann.lehman-at-trincoll.edu
w. http://www.trincoll.edu/~alehman



From daemon Wed Oct 18 12:39:54 2000



From: Edwards, Mark S (NINDS) :      yosh-at-codon.nih.gov
Date: Wed, 18 Oct 2000 13:35:05 -0500
Subject: JEOL 200CX specimen holder

Contents Retrieved from Microscopy Listserver Archives
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Some time back Gatan made a ±60+ single hole, non-rotary specimen holder
for the JEOL 200-CX. They are no longer in stock and I was hoping to
find one, or the equivalent, gathering dust somewhere that I could buy
and put to good use. Reply privately to {treese-at-marinebio.mbl.edu} . I
would also be interested in any surplus single tilt holders for the JEOL
that I could try to modify for ±60 tilt. Thanks!....Tom Reese "




From daemon Wed Oct 18 13:17:30 2000



From: Billfester-at-aol.com
Date: Wed, 18 Oct 2000 14:14:50 EDT
Subject: Re: light microscope lenses

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Steve,
The chief advantage to using water immersion lenses has to do with, as you
surmised, the refractive indices in the optical system. Live cells are most
happy in aqueous media, so therefore it is best to look at them in such an
environment. Coverslip corrected water lenses have the advantage of
eliminating oil from the system, and no-coverslip "dipping" lenses eliminate
the gross errors that can occur when looking through the unpredictable
surface of water (try to take a picture of a fish from a dock, then jump in
the water and try an underwater photo).

An air-water interface produces problems similar to an air-glass interface.
In the same way that an oil lens will optimize the image of a fixed prep, a
water immersion lens will optimize observation of an aqueous prep. The
resolution of your optical system is determined generally by the lowest
refractive index. Therefore it is beneficial to eliminate air from the
equation, but there is no advantage to using an oil lens with a water prep.
An oil lens may have higher potential NA, say 1.4 at 60X rather than 1.2 for
the water 60X, but the system NA is limited by the 1.33 refractive index of
water, not the 1.515 refractive index of the oil. The highest potential NA of
the water prep system is 1.33, just as an oil system is potentially 1.5, yet
practical design concerns result in about a 1.2 limit for the water prep, 1.4
for the oil.

An advantage to using a coverslip corrected water lens, your case #1, is that
the lens designers need only account for two refractive indicies when dealing
with spherical aberration, and these two will always be in similar
proportion. As these are live cells and somewhat unpredictable, we cannot
always know how close they will be to the coverglass. Between the cell and
the coverglass is water, and between the coverglass and lens is again water.
It does not matter for the system whether the cell is very close to the
coverslip or a little further away - there will just be a little more or less
water on the objective side. Thus the optical path through water and glass is
always the same. With oil immersion, the oil and water in the path will
change depending on whether the cell is relatively near or far from the
coverslip, as the objective moves closer or further from the coverglass.
This can result in spherical aberration. A sensitive instrument like a
confocal microscope is sensitive to these errors.

As for your case #2, non-coverslip corrected water lenses, or "dipping
lenses", are crucial for live cell experiments where a number of devices may
be manipulating or even connected to the cells. For one thing we have cells
in a dish with a number of devices penetrating the water from above, giving
an irregular surface. Additionally we have a sometimes significant amount of
buffer above the cells, giving rise to working distance issues. A dipping
lens typically has a narrow tapered end, teflon coated, and can be brought
nicely into the buffer. The NA is typically a little lower than a coverslip
objective but the needs for working distance (~2-3mm) and narrow profile
outweigh the need for very high NA (0.9 vs. 1.2 for a 60X). A dry or oil lens
simply will not perform in such conditions.

I hope this gives you a bit more background.

Regards,
Bill Fester
Olympus America
Microscope Division - NY

}
} Netters
}
} In upgrading our light microscopes, we are buying some new lenses for our
} confocal scope and a brightfield scope. The salesforce is regaling us with
} tales of improved resolution, clarity, brightness etc that comes with
} spending double or triple the price for a standard oil immersion or dry
} lens. Could someone please clarify, either on-line or off, what the
} advantages and disadvantages of each of the following ( my texts on light
} microscopy don't deal with them). Are they worth the extra dollars?
}
} 1) a water immersion lens for looking at cells suspended in buffer and
} covered with a coverslip (water is used rather than oil between coverslip
} and lens) These lenses typically have a lower numerical aperture than an
} oil immersion lens.
}
} 2) a water dipping lens, used to examine cells in culture directly, without
} a coverslip. I would expect the absence of refractive indice changes
} (media/coverslip/air or oil) would explain their improved brightness.
}
} Thanks in advance for your input
}
} Steve Barlow
} SDSU EM Facility
}
}




From daemon Wed Oct 18 13:42:50 2000



From: Won-Sik Kim :      kimwonsi-at-msu.edu
Date: Wed, 18 Oct 2000 14:39:52 -0400
Subject: jet thinning recipe for 1045 steel and 1070 steel

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello

I would appreciate any advice/recommendations on jet thinning
(electropolishing) recipe for 1045 steel and 1070 steel.
Thanks


Won-Sik Kim




From daemon Wed Oct 18 14:35:34 2000



From: Jo Ann Moore :      jamoore-at-hsc.usf.edu
Date: Wed, 18 Oct 2000 15:39:57 -0400
Subject: need J mount adapter

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Fellow microscopists:

I am posting this request to help Paul, a graduate student here. If
anyone can help him out, please respond to me.

Thanks,
Jo Ann Moore
Senior Biological Scientist
Anatomy Department, College of Medicine
University of South Florida
Tampa FL


Hello folks -

I am in the process of refurbishing an old Aus-Jena microscope in our
laboratory, and would like to be able to connect a video camera to the
camera mount, but unfortunately it seems nearly impossible to find an
appropriate J-mount adapter anywhere costing less than my left leg and
right hand. I am hoping that one of the photomicroscopists around
might have a spare one of these squirreled away in a drawer or
cabinet somewhere that they wouldn't mind parting with. And if there's
a disabled Aus-Jena scope around that could be available for parts,
that'd be great as well.

Many thanks,
Paul Jantzen
Alzheimer's Research Laboratory






From daemon Thu Oct 19 11:41:16 2000



From: Phoebe J Doss :      pjdoss-at-okstate.edu
Date: Thu, 19 Oct 2000 11:23:56 -0500
Subject: Oxford/Link exL system

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello:

I have an Oxford/Link exL WDS/EDX (detector not included) system that I
will gladly send to anyone for cost of shipping. The monitor and printer
are still operational (or was the last time I could get the system to boot
up), the rest does not work at all. I also have a monitor to a Tracor 2000
system is anyone needs it.

Phoebe Doss
Manager
Electron Microscope Lab
Oklahoma State University




From daemon Thu Oct 19 17:55:32 2000



From: Chuck Buiocchi :      buiocchi-at-astro.ocis.temple.edu
Date: Thu, 19 Oct 2000 17:45:31 -0500
Subject: HELP WITH JEOL 100CX II

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Good Morning,
We moved the Instrument( JEOL 100CX II ) down the hall. Now the Diff. Pump
Light comes on for the INITIAL WARM-UP, But after ~ 20min, the Diffusion
Pump light goes OFF, and after another 10 min. the Scope shuts down.
We checked & rechecked all the cables connections, didn't help.
ANY IDEAS!!!!!
Thanks
Chuck





From daemon Thu Oct 19 20:06:56 2000



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Thu, 19 Oct 2000 15:00:31 -1000 (HST)
Subject: Re: HELP WITH JEOL 100CX II

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi, Chuck-

} We moved the Instrument( JEOL 100CX II ) down the hall. Now the Diff. Pump
} Light comes on for the INITIAL WARM-UP, But after ~ 20min, the Diffusion
} Pump light goes OFF, and after another 10 min. the Scope shuts down.
} We checked & rechecked all the cables connections, didn't help.
} ANY IDEAS!!!!!

Here's one - clean the penning gauge and see if it behaves.

Aloha,
Tina

http://www.pbrc.hawaii.edu/bemf/microangela

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************




From daemon Thu Oct 19 21:56:24 2000



From: COURYHOUSE-at-aol.com
Date: Thu, 19 Oct 2000 22:50:26 EDT
Subject: Re: HELP WITH JEOL 100CX II

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Is it level?
Ed Sharpe archivist for SMECC

{ { Subj: HELP WITH JEOL 100CX II
Date: 10/19/00 6:26:30 PM US Mountain Standard Time
From: buiocchi-at-astro.ocis.temple.edu (Chuck Buiocchi)
To: Microscopy-at-sparc5.microscopy.com

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Good Morning,
We moved the Instrument( JEOL 100CX II ) down the hall. Now the Diff. Pump
Light comes on for the INITIAL WARM-UP, But after ~ 20min, the Diffusion
Pump light goes OFF, and after another 10 min. the Scope shuts down.
We checked & rechecked all the cables connections, didn't help.
ANY IDEAS!!!!!
Thanks
Chuck


} }



From daemon Fri Oct 20 00:07:38 2000



From: Ronald Vane :      RVaneXEI-at-concentric.net
Date: Thursday, October 19, 2000 4:52 PM
Subject: HELP WITH JEOL 100CX II

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Chuck:

I haven't worked with the 100 CX but I am familiar with the JEOL way of
doing things.

The diffusion pump LED is a warning lamp (assuming the usual JEOL way) and
probably indicates that the cooling water or diff pump power is off. Check
to see if the diffusion pump is heating and that you have water flow. I
suspect bad water flow is keeping the pump heater from being turned on. The
other 10 minutes to shut down is just another JEOL safety circuit. If the
vacuum system is stays in rough pumping mode, the scope turns off after 30
minutes.

Ron Vane
XEI Scientific

-----Original Message-----
} From: Chuck Buiocchi {buiocchi-at-astro.ocis.temple.edu}
To: Microscopy-at-sparc5.microscopy.com {Microscopy-at-sparc5.microscopy.com}




From daemon Fri Oct 20 02:17:12 2000



From: Robin Cross :      r.cross-at-ru.ac.za
Date: Fri, 20 Oct 2000 09:10:09 +0200
Subject: Re: HELP WITH JEOL 100CX II

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Chuck

} We moved the Instrument( JEOL 100CX II ) down the hall. Now the Diff.
} Pump Light comes on for the INITIAL WARM-UP, But after ~ 20min, the
} Diffusion Pump light goes OFF, and after another 10 min. the Scope
} shuts down. We checked & rechecked all the cables connections, didn't
} help. ANY IDEAS!!!!!

Sounds to me as if a connection to one of the diff pump heaters
has broken. The wires become brittle from the heat and a bump
while you were moving it make have broken one of them. A more
costly possibility is that one of the heaters may have expired.

Regards

Rob





Robin H Cross
Director : EM Unit, Rhodes University, Grahamstown, South Africa
R.Cross-at-ru.ac.za
tel: +27 46 603 8168 - fax: +27 46 622 4377
http://www.ru.ac.za/emu/index.htm

** remember that ICEM-15 in 2002 is in Durban, South Africa **



From daemon Fri Oct 20 02:55:42 2000



From: Vitaly Feingold :      vitalylazar-at-worldnet.att.net
Date: Thursday, October 19, 2000 8:39 PM
Subject: HELP WITH JEOL 100CX II

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Chuck,
For the luck of details in your description- is the cooling water runs
properly through all circuits? Both diff. pumps, electronics, lenses?
Perhaps some line(s) (partially) clogged? Check 2 solenoid water valves on
the water hoses on the floor behind the TEM. Are the wires broken? Those are
easy to damage during the move.
Feel sides of the diff. pumps and electronics return water line 15 minutes
after start up. Are they getting hot?
Flowmeter in the water line never hurts...

Vitaly Feingold
Scientific Instruments and Applications
2773 Heath Lane, Duluth GA 30096
(770)232-7785 ph.
(770)232-1791 fax

This message is made of 100% recycled electrons.
-----Original Message-----
} From: Chuck Buiocchi {buiocchi-at-astro.ocis.temple.edu}
To: Microscopy-at-sparc5.microscopy.com {Microscopy-at-sparc5.microscopy.com}




From daemon Fri Oct 20 03:04:41 2000



From: Dan Kaszubski :      danzk-at-oz.sunflower.org
Date: Fri, 20 Oct 2000 03:01:30 -0500
Subject: RE: HELP WITH JEOL 100CX II

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Chuck,
This could be a cooling problem; first DP. overheats and trips off, then a
part of electronics - lens coils or power supply (whatever is cooled in your
scope) -this would shut down the scope completely.

Good luck!
Dan


Dan Kaszubski
Micro-Time Co.
Olathe,KS


Good Morning,
We moved the Instrument( JEOL 100CX II ) down the hall. Now the Diff. Pump
Light comes on for the INITIAL WARM-UP, But after ~ 20min, the Diffusion
Pump light goes OFF, and after another 10 min. the Scope shuts down.
We checked & rechecked all the cables connections, didn't help.
ANY IDEAS!!!!!
Thanks
Chuck







From daemon Fri Oct 20 06:21:13 2000



From: Keith Ryan :      kpr-at-pml.ac.uk
Date: Fri, 20 Oct 2000 12:14:54 +0100
Subject: Re: HELP WITH JEOL 100CX II

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Chuck

I had a similar problem recently (SEM) with a JSM 35C, it pumped for
20-30 minutes and then cut out.
Hints:
1. make sure the water flow is ok
2. make sure the diff pumps are getting hot
3. make sure the RP belts are tight.
My JSM problem was a loose RP belt on the pump which 'roughs' the
column - the pre-vac. in the column wasn't good enough. A new belt
cured it.

But I still have the same problem on a 200CX TEM and it's none of the
above - awaiting help from the answers to your original question! Let
me know of any good ones you might get off-line, please!

Keith Ryan
Marine Biological Association
Plymouth UK

{ { { Chuck Buiocchi {buiocchi-at-astro.ocis.temple.edu} 10/20 4:23a } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of
America


Good Morning,
We moved the Instrument( JEOL 100CX II ) down the hall. Now the Diff.
Pump
Light comes on for the INITIAL WARM-UP, But after ~ 20min, the
Diffusion
Pump light goes OFF, and after another 10 min. the Scope shuts down.
We checked & rechecked all the cables connections, didn't help.
ANY IDEAS!!!!!
Thanks
Chuck








From daemon Fri Oct 20 06:48:50 2000



From: Phillip Rutledge :      prutledge-at-ars.usda.gov
Date: Fri, 20 Oct 2000 05:41:23 -0600
Subject: Re: HELP WITH JEOL 100CX II

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} } } Chuck Buiocchi {buiocchi-at-astro.ocis.temple.edu} 10/20/00 00:19 AM } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Good Morning,
We moved the Instrument( JEOL 100CX II ) down the hall. Now the Diff. Pump
Light comes on for the INITIAL WARM-UP, But after ~ 20min, the Diffusion
Pump light goes OFF, and after another 10 min. the Scope shuts down.
We checked & rechecked all the cables connections, didn't help.
ANY IDEAS!!!!!
Thanks
Chuck

Chuck,

I used a 100 CX and when thsat happened to me, it was usually the top diff pump heater. They go bad after a while. In 13 years I've only replaced the bottom diff
pump heater 1 time. I've never used a CXII so I don't know if it has 2 diff pumps. Hope this helps.

Phil Rutledge





From daemon Fri Oct 20 08:55:01 2000



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Fri, 20 Oct 2000 08:48:48 -0500
Subject: HELP WITH JEOL 100CX II

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We had a similar problem recently, but it turned out to be my fault, rather
than the scope's. When you said you had just moved the scope, it rang a
bell...

I didn't move the scope, but I rearranged the position of its water chiller.
A couple days later, the scope was shutting down and the chiller was
laboring mightily with a water temp of over 100 F. I checked the water
level and refrigerant level and internal water flow in the chiller tank and
all seemed well. So I called the refrigeration guy.

After looking around a bit, the first thing he says is, "Did you move this
recently?" I said yes, and he felt behind the chiller and found two hoses
that had crimped when I moved it against the wall. Poor thing was hardly
getting any outside cooling water flow.

The refrigeration guy was kind. He didn't make fun of me and he didn't
charge me for the visit.

Good luck,
Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/




-----Original Message-----
} From: Chuck Buiocchi [mailto:buiocchi-at-astro.ocis.temple.edu]
Sent: Thursday, October 19, 2000 5:46 PM
To: Microscopy-at-sparc5.microscopy.com


Good Morning,
We moved the Instrument( JEOL 100CX II ) down the hall. Now the Diff. Pump
Light comes on for the INITIAL WARM-UP, But after ~ 20min, the Diffusion
Pump light goes OFF, and after another 10 min. the Scope shuts down.
We checked & rechecked all the cables connections, didn't help.
ANY IDEAS!!!!!
Thanks
Chuck





From daemon Fri Oct 20 10:31:51 2000



From: Ron Doole :      ron.doole-at-materials.oxford.ac.uk
Date: Fri, 20 Oct 2000 16:25:36 +0100 (BST)
Subject: Re: HELP WITH JEOL 100CX II

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On Fri, 20 Oct 2000, Keith Ryan wrote:
Hi There,

Have you checked the compressed air pressure or the pressure switch. I
don't have drawings but I assume that one is fitted.

Good luck,
Ron

}
} Chuck
}
} I had a similar problem recently (SEM) with a JSM 35C, it pumped for
} 20-30 minutes and then cut out.
} Hints:
} 1. make sure the water flow is ok
} 2. make sure the diff pumps are getting hot
} 3. make sure the RP belts are tight.
} My JSM problem was a loose RP belt on the pump which 'roughs' the
} column - the pre-vac. in the column wasn't good enough. A new belt
} cured it.
}
} But I still have the same problem on a 200CX TEM and it's none of the
} above - awaiting help from the answers to your original question! Let
} me know of any good ones you might get off-line, please!
}
} Keith Ryan
} Marine Biological Association
} Plymouth UK
}
} { { { Chuck Buiocchi {buiocchi-at-astro.ocis.temple.edu} 10/20 4:23a } } }
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Good Morning,
} We moved the Instrument( JEOL 100CX II ) down the hall. Now the Diff.
} Pump
} Light comes on for the INITIAL WARM-UP, But after ~ 20min, the
} Diffusion
} Pump light goes OFF, and after another 10 min. the Scope shuts down.
} We checked & rechecked all the cables connections, didn't help.
} ANY IDEAS!!!!!
} Thanks
} Chuck
}
===========================================================================
Mr. Ron Doole e-mail ron.doole-at-materials.ox.ac.uk
Department of Materials, phone +44 (0) 1865 273701
University of Oxford, fax +44 (0) 1865 283333
Parks Road.
Oxford. OX1 3PH. UK.
============================================================================




From daemon Fri Oct 20 10:52:27 2000



From: Massimo Catalano :      massimo.catalano-at-ime.le.cnr.it
Date: Fri, 20 Oct 2000 17:48:13 +0200
Subject: 5th Multinational Congress on Electron Microscopy - First

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Colleagues,

please find enclosed a summary of the first circular of the 5th
Multinational Congress on Electron Microscopy, that will be held in Lecce
(ITALY), on September 20-25 2001.

An overseas participation is strongly desired, and we hope that many
scientists from all over the world will come to Italy in September 2001.

The congress is organized by the following societies:

Austrian Society for Electron Microscopy
Croatian Society for Electron Microscopy
Czechoslovak Society for Electron Microscopy
Hungarian Society for Microscopy
Italian Society for Electron Microscopy
Slovenian Society for Electron Microscopy

The purpose of the congress is to provide an interdisciplinary forum where
scientists, both in the materials science and in the biomedical research
can present the most recent results of their activity.

Some of the topics which will be covered are:

Architecture of the nucleus and nuclear import and export,
art, biomaterials, biosensors, environmental microscopy,
cell and cell components, cell death in biology and pathology,
chemical analysis and mapping, microscopy in human health,
microscopy of nanostructural materials,
microscopy of structural and functional materials,
energy-filtered electron microscopy,
instrumental development, investigations of thin films for sensor technology,
microbiology, microscopy of semiconducting materials,
parassitology, plant cell biolgy, remote microscopy,
strain analysis, structure biology,
structural determination and refining, viruses,
teleoperation and computer assisted operation,
nanotechnology, single molecule microscopy and manipulation.

You can find further information and eventually download the brochures
(soon available) and also preregister at the web site:

www.mcem5.unile.it

The Congress contacts are:

Laboratory of Comparative Anatomy and Cytology
Dept. of Biology
University of Lecce
Via per Monteroni, 73100 Lecce, Italy
Phone: +39 0832 320657 (855)
Fax: +39 0832 320654


Institute for the study of new Materials for Electronics
University of Lecce
Via per Arnesano, 73100 Lecce, Italy
Phone: +39 0832 322362
Fax: +39 0832 325299

Thanks for your attention

Massimo






From daemon Fri Oct 20 11:55:31 2000



From: William Carmichael :      wcarmichael-at-madison.tec.wi.us
Date: Fri, 20 Oct 2000 11:45:06 -0500
Subject: re:JEOL 100CX help

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Chuck,

The JEOL scopes have a heat sensor that ensures the DP is hot. They sometimes have two sensors, one at the bottom of the DP, the hottest point, and one at the top of the DP the coolest point. My guess is during your move the sensor(s) came out of the clamp that holds it against the DP. Look for a cylinder that has two wires coming out of it and find the metal band or clamp where it fits into. I hope this helps!

Bill


_______________________________

Bill Carmichael
Electron Microscopy Faculty

Madison Area Technical College
3550 Anderson St.
Madison, WI 53704
608-243-4309

wcarmichael-at-madison.tec.wi.us
http://electron-microscopy.madison.tec.wi.us





From daemon Fri Oct 20 14:26:50 2000



From: anderron-at-us.ibm.com
Date: Fri, 20 Oct 2000 15:19:06 -0400
Subject: Light Microscope Prospective Purchaser

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


This e-mail came in to the MSA Business Office. If you sell light
microscopes you will want to respond directly to the writer. Please do not
copy the listserver.

Ron

} } } } } }

To whom this may concern:
I am Karim El Masry, Assistant Brand Manager on Haircare products in
Procter &
Gamble Egypt.

I was wondering if you could help me find video microscopes that magnify up
to
x200. I will be using the microscopes in one of my programs demonstrating
the
video microscopes capabilities.

Please advise with the company producing or supplying them. I will need at
least a good 50 microscopes within the next two weeks.

Hope to hear from you very soon.
Best Regards,
Karim
+202 394 7609

{elmasry.k-at-pg.com}

} } } } } } } }



Ron Anderson, IBM, Hopewell Jct., New York, USA. anderron-at-us.ibm.com

IBM Analytical Services; http://www.chips.ibm.com/services/asg




From daemon Fri Oct 20 15:03:00 2000



From: Tom Phillips :      PhillipsT-at-missouri.edu
Date: Fri, 20 Oct 2000 14:54:43 -0500
Subject: hardness of epon resins vs stability and cutting ability

Contents Retrieved from Microscopy Listserver Archives
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Does a "hard" formulation of an epon resin translate into a block
that can be cut thinner?

Is the "hard" formulation more stable in the electron bean since it
is more cross-linked?

TIA, tom

--
Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)



From daemon Fri Oct 20 15:09:14 2000



From: Jo Dee Fish :      jofish-at-burnham-inst.org
Date: Fri, 20 Oct 2000 13:03:05 -0700
Subject: LKB parts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear List,
Does anyone out there know where I can order parts for my LKB Ultrotome
V?
I need to order new chucks and can't find a dealer who deals with the
LKB's. Are parts available anymore? A textbook listed Cambridge
Instruments as a supplier. Anyone have a phone, e-mail or address?
Thanks for your help,
Jo Dee

--
Jo Dee Fish
Coordinator of Electron Microscopy
Cell Analysis Facility
The Burnham Institute
10901 N. Torrey Pines Rd.
La Jolla, CA 92037
(858)646-3100 ext. 3620





From daemon Fri Oct 20 17:15:25 2000



From: Debby Sherman :      sherman-at-btny.purdue.edu
Date: 20 Oct 2000 17:08:23 -0500
Subject: In situ for LM

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The following is a brief note from a graduate student who is going to try in situ localization on plant tissue for light microscopy. Any help you can give her is appreciated. Send replies to me and I will forward to her.
Thanks in advance for your help.
Debby
------
I am planning in situ hybridization using ribo-probes labelled with DIG with maize and rice tissues (coleoptiles, young leaves, roots and root tips). I am afraid that some of the organs like the root tips are very small to be embedded in paraffin and still be able to get nice cross sections. Is there any other resin that I can try?
Also the fixative that I planned to use consists of 50% methanol, 5% acetic acid, and 2% formaldehyde (40%). Do you have other suggestions for a fixative. I would appreciate any protocols which may help in this project.
Claudia Elena Vergara

----
Debby Sherman Phone: 765-494-6666
Life Science Microscopy Center FAX: 765-494-5896
c/o Dept.of Botany & Plant Path. E-mail: sherman-at-btny.purdue.edu
Purdue University
1057 Whistler Building
West Lafayette, IN 47907-1057




From daemon Fri Oct 20 18:53:05 2000



From: Chuck Buiocchi :      buiocchi-at-astro.ocis.temple.edu
Date: Fri, 20 Oct 2000 18:46:05 -0500
Subject: HELP WITH JOEL 100CX II

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Good Morning Everyone,
First let me say THANK YOU, to all who responsed to my earlier message,
greatly appreciated!!!
However some NEW additional info.
The pump is HEATING up, but the Scope dies after it switches over to pump
out the colum.
Any Ideas???
Thanks again,
Chuck





From daemon Fri Oct 20 18:53:06 2000



From: Gary M. Easton :      gary.easton-at-scannerscorp.com
Date: Fri, 20 Oct 2000 18:46:21 -0500
Subject: JSM-35 Vac controller

Contents Retrieved from Microscopy Listserver Archives
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Listers, Anyone out there with a spare vac power supply & vac
controller board for a JSM 35 SEM that they would be willing to sell?
JEOL wasn't much help. Please reply off line, either by email or phone.
Thanks in advance. Gary M. Easton Scanners Corporation 410-857-7633 x102





From daemon Fri Oct 20 23:08:24 2000



From: Gillian Bond :      gbond-at-nmt.edu
Date: Fri, 20 Oct 2000 21:58:42 -0600
Subject: Ceramic powders

Contents Retrieved from Microscopy Listserver Archives
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Hello to all on the Listserver,

A colleague's student came and asked me how to prepare a ceramic powder for
TEM observation. As I understand it, the powder is composed of alumina
particles, average size 90 microns. The particles are very porous, with
holes/pores ranging in size from around 1 to 30 microns. Their aim is to
show the presence of internal pores. And yes they have done SEM on
fractured particles, but they have been asked to do TEM as well.

Since these samples are rather different from what I generally work with,
I'm hoping that someone can give us some advice on the specimen prep.

Many thanks in advance for any help you can give us.

Gill

Dr. Gillian M. Bond
Department of Materials & Metallurgical Engineering
New Mexico Tech
Socorro, NM 87801




From daemon Sat Oct 21 05:37:14 2000



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Sat, 21 Oct 2000 10:56:09 -0500
Subject: Ceramic powder preparation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Leica may be able to help you
Their US and UK addresses are copied below

The following web site has these and other microscopy vendor
addresses, phone numbers, emails and links to their web pages

http://www.kaker.com/mvd/vendors.html
Chris

Leica Instruments, Inc.
111 Deer Lake Road
Deerfield, IL 60015
USA
Tel: 800 248 0123, 708 405 0123
Fax: 708 405 8139
Leica UK Ltd
Davy Avenue
Knowlhill, Milton Keynes MK5 8LB
UK
Tel: +44 (0) 1908 24 62 46
Fax: +44 (0) 1908 60 99 92

Date sent: Fri, 20 Oct 2000 13:03:05 -0700
} From: Jo Dee Fish {jofish-at-burnham-inst.org}
To: Microscopy-at-sparc5.microscopy.com


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Gillian M. Bond wrote:
================================================================
A colleague's student came and asked me how to prepare a ceramic powder for
TEM observation. As I understand it, the powder is composed of alumina
particles, average size 90 microns. The particles are very porous, with
holes/pores ranging in size from around 1 to 30 microns. Their aim is to
show the presence of internal pores. And yes they have done SEM on
fractured particles, but they have been asked to do TEM as well.

Since these samples are rather different from what I generally work with,
I'm hoping that someone can give us some advice on the specimen prep.

Many thanks in advance for any help you can give us.
==================================================================
We have been preparing these types of samples in our own laboratory for a
number of years for our commercial clients. We have found that such samples
,

1] Have to be vacuum embedded in order to make sure all the internal pores
are infiltrated with the resin,

2] Diamond knife ultramicrotomy is always required, because glass knives
just won't "cut" it, and

3] We prefer our own SPI-Pon™ 812 resin (although at least some of the
other "Epon substitute" resins should work just as well), in part because
you have the possibility of varying the hardness of the final resin through
a considerable range, a feature sometimes important in terms of ending up
with a block with the "right" properties (e.g. hardness).

4] If these 90 µm particles were formed in situ, and not as the result of
grinding, then they should be sputter coated with (preferably) Pt but Au
will do if Pt is not available, prior to embedding, so that once in the TEM,
one would always be able to keep track of the original outside surfaces,
since this is the way one can determine the presence of a skin (of different
morphology).

Contrast can be a problem for resolving the pores of the smallest dimensions
, so one would probably be wise to think about the use of "holey" support
films. We prefer our own SPI Materials Science Diamond Knives for this type
of work because these kinds of samples will immediately impart to the knife
edge the kinds of fine striations one works so hard to get out in the
production of a more expensive "life science" diamond knife. In other words
, using a new life science knife on these kinds of samples (in our opinion)
is quite wasteful economically.

Disclaimer: SPI Supplies offers the embedding resins, diamond knives and
holey support films for doing this kind of work. We also provide this kind
of laboratory service for those not set up or wanting to do their own
sample preparation.

Chuck
============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.spi.cc
########################
============================================










From daemon Sat Oct 21 15:53:16 2000



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Sun, 22 Oct 2000 09:47:03 GMT+1200
Subject: Re: HELP WITH JOEL 100CX II

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



}
}
} Good Morning Everyone,
} First let me say THANK YOU, to all who responsed to my earlier
} message, greatly appreciated!!! However some NEW additional info.
} The pump is HEATING up, but the Scope dies after it switches over to
} pump out the colum. Any Ideas??? Thanks again, Chuck
}
}
}

Is that after starting to evacuate via the rotary pump, or later,
when it switches over to the DP?

And does it switch off as soon as it starts the column evacuation, or
after a while?

Maybe you've got such a massive air leak that the watchdog senses
that there's not sufficient vacuum backing for the DP(s).

cheers

rtch


Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand



From daemon Sun Oct 22 15:32:52 2000



From: tonino traini :      ttrain-at-tin.it
Date: Sun, 22 Oct 2000 15:19:57 -0500
Subject: used microtome wanted for purchase

Contents Retrieved from Microscopy Listserver Archives
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Hello folks, I am looking for a used microtome which will work well
in sectioning
resin (1-3 micron). A manual microtome would be fine, especially if is a
good bargain. Thanks!

Tonino Traini CDT-MDT-DDS
Electron Microscopy Center Ud'A University
* Tel: (871) 3554143
* Fax: (735) 90514
* E-mail: ttrain-at-tin.it






From daemon Mon Oct 23 02:00:36 2000



From: Robin Cross :      r.cross-at-ru.ac.za
Date: Mon, 23 Oct 2000 08:45:55 +0200
Subject: 100CX II

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Hello Chuck

} The pump is HEATING up, but the Scope dies after it switches
} over to pump out the colum. Any Ideas???

There are two diff pumps - are they both heating?

If both are heating I think the most likely possibility, as mentioned
by others here in response to your original enquiry, is overheating of
the diff pumps because of a water cooling problem. On the other
hand, if the instrument turns off at or very soon after it tries to
switch over to high vacuum, and if the diff pumps are hot but not
overheating, then it sounds as if the rough pumping by the rotary
pump has been inadequate. Is there any pumping noise from the
rotary pumps? It may be that one of the hoses was not properly re-
connected after the move, or one of the hoses may be leaking.
They perish after a while.

Good luck.

Rob





Robin H Cross
Director : EM Unit, Rhodes University, Grahamstown, South Africa
R.Cross-at-ru.ac.za
tel: +27 46 603 8168 - fax: +27 46 622 4377
http://www.ru.ac.za/emu/index.htm

** remember that ICEM-15 in 2002 is in Durban, South Africa **
------- End of forwarded message -------

Robin H Cross
Director : EM Unit, Rhodes University, Grahamstown, South Africa
R.Cross-at-ru.ac.za
tel: +27 46 603 8168 - fax: +27 46 622 4377
http://www.ru.ac.za/emu/index.htm

** remember that ICEM-15 in 2002 is in Durban, South Africa **



From daemon Mon Oct 23 07:55:44 2000



From: ed_bachmann-at-unc.edu
Date: Mon, 23 Oct 2000 07:47:54 -0500
Subject: Looking for a high quality stereoscopic dissecting scope

Contents Retrieved from Microscopy Listserver Archives
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Email: ed_bachmann-at-unc.edu
Name: Ed Bachmann
School: University of North Carolina at Chapel Hill

Question: I am looking for a high quality new or used stereoscopic
dissecting scope for examining mosses. I would like as high a
magnification as I can get consistent with very good resolution. Is there
anyplace I can find unbiased reviews or comparisons of brands and models?
Can you comment on the use of adaptor lenses that attach to the objective
lens to boost magnification. I've been told they cause image degradation
(loss of resolution?). Thank you.

---------------------------------------------------------------------------





From daemon Mon Oct 23 09:17:01 2000



From: Bob Bagnell :      rml-at-grayhawk.med.unc.edu
Date: Mon, 23 Oct 2000 10:12:54 -0400
Subject: TEM Digital Cameras - More Resolution

Contents Retrieved from Microscopy Listserver Archives
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We have begun using a Gatan Bio-Scan digital camera on our LEO
EM910 and we like it very much. I would like to improve on this camera's
capabilities by increasing its spatial resolution. One method I've heard of
and that is used in some digital cameras is to acquire multiple images at
sub-pixel spacing and then recombine these into one image. My microscope
has the capability of shifting the image in any direction in sub-pixel
units. Has anyone tried this, or does anyone know where I can get
information on how this might be done?

_____________________________________________________________
C. Robert Bagnell Jr., Ph.D., Res.Assoc.Prof.
Microscopy Services Laboratory
Department of Pathology & Laboratory Medicine
CB #7525 UNC-CH, Chapel Hill, N.C. 27599
ph 919-966-2413 fx 919-966-6718
http://www.pathology.med.unc.edu/path/microscopy/welcome.htm





From daemon Mon Oct 23 09:28:46 2000



From: Bill Miller :      microbill-at-mohawk.net
Date: Mon, 23 Oct 2000 10:24:54 +0100
Subject: PhotoShop usage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I was talking to a friend of mine this morning about what software he
should have to do digital imaging. Reflexively I said PhotoShop and was
asked how he would use it. I told him the standard things - contrast
enhancement, color matching across different media, annotation, resizing,
etc. His response was that for the price was that all it did (a little
naivety but a fair question) . You know, sometimes you get in a rut and it
set me to wondering how other people are using PhotoShop? It's not like
I'm selling the software but I'd like to be able to give a better answer
before telling someone they should plunk down that much money. Aside from
image analysis plug-ins (which I had forgotten about when we were talking)
how else is PhotoShop being used - anything fancy to justify the cost?

Bill Miller




From daemon Mon Oct 23 09:44:54 2000



From: Mati Raudsepp :      raudsepp-at-unixg.ubc.ca
Date: Mon, 23 Oct 2000 07:39:14 -0700
Subject: Contact info for Cambridge Image Technology Cathodoluminescence

Contents Retrieved from Microscopy Listserver Archives
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Dear Colleagues:

We are trying to track down the location of "Cambridge Image Technology,"
manufacturers of cathodoluminescence stages for optical microscopes. Can
anyone help? Thanks in advance,

Mati

Mati Raudsepp, Associate Professor (Hon.)
Dept. of Earth & Ocean Sciences
6339 Stores Road
The University of British Columbia
Vancouver, BC V6T 1Z4

Tel: 604 822-6396
Fax: 604 822-6088




From daemon Mon Oct 23 10:21:08 2000



From: Walck, Scott D. :      walck-at-ppg.com
Date: Mon, 23 Oct 2000 11:13:41 -0400
Subject: RE: PhotoShop usage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


You should add the ability to do montaging with layers, selecting and
adjusting contrast ranges, selecting edges. you also have the capability of
using John Russ' Image Processing Toolkit or Provea Pro to do relatively
inexpensive image analysis.


-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)



} -----Original Message-----
} From: Bill Miller [mailto:microbill-at-mohawk.net]
} Sent: Monday, October 23, 2000 5:25 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: PhotoShop usage
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListse} rver/FAQ.html
}
}
} --------------------------------------------------------------
} ---------.
}
}
} I was talking to a friend of mine this morning about what software he
} should have to do digital imaging. Reflexively I said
} PhotoShop and was
} asked how he would use it. I told him the standard things - contrast
} enhancement, color matching across different media,
} annotation, resizing,
} etc. His response was that for the price was that all it did
} (a little
} naivety but a fair question) . You know, sometimes you get
} in a rut and it
} set me to wondering how other people are using PhotoShop?
} It's not like
} I'm selling the software but I'd like to be able to give a
} better answer
} before telling someone they should plunk down that much
} money. Aside from
} image analysis plug-ins (which I had forgotten about when we
} were talking)
} how else is PhotoShop being used - anything fancy to justify the cost?
}
} Bill Miller
}
}



From daemon Mon Oct 23 12:46:12 2000



From: Anthony Garratt-Reed :      tonygr-at-mit.edu
Date: Mon, 23 Oct 2000 13:33:38 -0400
Subject: Re: PhotoShop usage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


One of the reasons that Photoshop is in such widespread use may be Adobe's
sales policy. Like Apple years ago, they sell to Academic Institutions at
very greatly discounted prices, so that for us we aren't "plunking down
that much money" - in fact, the cost to us of Photoshop is competitive with
far less able program suites. As a result, all our graduates go off into
their jobs and immediately buy Photoshop!

In reality, though, I would still consider Photoshop because it has
everything I could possibly need (other, cheaper packages are almost always
deficient in some way or another), because it will handle 16 bit images,
because its user interface is not frilled up but is fully accessible,
because of the extensive file format support, and so on, as well, of
course, as the plug-ins. Although Photoshop has power that I will never
ever use, I'm also surprised at how often I use some feature that I would
never have considered as important, if I did a feature by feature
price-performance evaluation of cheaper products.

Tony Garratt-Reed

At 10:24 AM 10/23/2000 +0100, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

** Anthony J. Garratt-Reed
** MIT Room # 13-1027
** 77 Massachusetts Avenue
** Cambridge, MA 02139-4307
** USA
**
** Phone: (+) 1-617-253-4622
** Fax: (+) 1-617-258-6478
**





From daemon Mon Oct 23 13:21:17 2000



From: Tamara Howard :      howard-at-cshl.org
Date: Mon, 23 Oct 2000 14:12:54 -0400 (EDT)
Subject: Job post - Core Technician (biology)

Contents Retrieved from Microscopy Listserver Archives
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This is a re-post; please excuse us if you've seen it before.

Also, please respond to Dr. Spector (e-mail given in post), not to the
poster...although I will forward any messages I receive on to him.
+++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++

Microscopy Core Technician -

Cold Spring Harbor Laboratory is seeking an experienced and responsible
microscopy technician for the laboratory's core biological microscopy
facility. The individual should have practical expertise in transmission
electron microscopy, confocal and widefield fluorescence microscopy,
digital imaging, and microinjection. The successful candidate will be
involved in designing and carrying out experimental protocols for users,
training individuals in the use of various microscopes, and aligning
microscopes and keeping the facility operating at an efficient and high
level of productivity. Interested individuals should send their resume,
including a description of their expertise and the names and addresses of
3 references to: Dr. David L. Spector, Cold Spring Harbor Laboratory, One
Bungtown Road, Cold Spring Harbor, New York 11724, email:
spector-at-cshl.org




From daemon Mon Oct 23 13:45:03 2000



From: Paul Webster :      pwebster-at-mailhouse.hei.org
Date: 23 Oct 00 11:46:29 -0700
Subject: Subscribe

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------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America




Paul Webster, Ph.D
House Ear Institute
2100 West Third Street
Los Angeles, CA 90057
phone:213 273 8026
fax: 213 413 6739
e-mail: pwebster-at-hei.org
http://www.hei.org/htm/aemi.htm




From daemon Mon Oct 23 14:19:05 2000



From: Ekstrom, Harry :      harry.ekstrom-at-honeywell.com
Date: Mon, 23 Oct 2000 12:12:13 -0700
Subject: PhotoShop usage

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One nice action we do with PhotoShop is an automated process that compresses
a large tiff file or similar into an e-mailable jpeg. In the automated
batch process, it can compress a whole folder of large files in a matter of
a minute or two.

Harry Ekstrom
Materials Laboratory

(602) 231-2744
e-mail: harry.ekstrom-at-honeywell.com


-----Original Message-----
} From: Bill Miller [mailto:microbill-at-mohawk.net]
Sent: Monday, October 23, 2000 2:25 AM
To: Microscopy-at-sparc5.microscopy.com


I was talking to a friend of mine this morning about what software he
should have to do digital imaging. Reflexively I said PhotoShop and was
asked how he would use it. I told him the standard things - contrast
enhancement, color matching across different media, annotation, resizing,
etc. His response was that for the price was that all it did (a little
naivety but a fair question) . You know, sometimes you get in a rut and it
set me to wondering how other people are using PhotoShop? It's not like
I'm selling the software but I'd like to be able to give a better answer
before telling someone they should plunk down that much money. Aside from
image analysis plug-ins (which I had forgotten about when we were talking)
how else is PhotoShop being used - anything fancy to justify the cost?

Bill Miller




From daemon Mon Oct 23 14:40:32 2000



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Tue, 24 Oct 2000 08:42:33 GMT+1200
Subject: Re: Contact info for Cambridge Image Technology Cathodoluminesce

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



}
} We are trying to track down the location of "Cambridge Image
} Technology," manufacturers of cathodoluminescence stages for
} optical microscopes. Can anyone help? Thanks in advance,
}
} Mati

Don't know that one, but we've been happy with the Luminoscope we got
from Premier American Technologies, PA, phone 814/867-8600.

cheers

rtch


Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand



From daemon Mon Oct 23 15:02:34 2000



From: Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 23 Oct 2000 12:59:53 -0700
Subject: Re: TEM Digital Cameras - More Resolution

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Easy. Take multiple images with each having some
amount of overlap. Then feed these images into Visual
Stitcher Pro ($50) and voile, one single big image.

gary

At 07:12 AM 10/23/00, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From daemon Mon Oct 23 15:03:49 2000



From: Wil Bigelow :      bigelow-at-engin.umich.edu
Date: Mon, 23 Oct 2000 15:05:17 -0400
Subject: Setting the record straight ...

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Hi Listers:
Since all of us are heavily involved with the use of the internet, I
thought this message I just received from Prof. Galler, one of the true
computer experts here at the Univ. of Michigan, and one of the poeople who
has been deeply involved in the development of our computer system, might
be of interest to you. It not only sheds light on some of the political
aspects of the development of the internet, but also shows how risky it can
be to deal with news media.

I know this has some political overtones (and perhaps undertones, too)
However, I think it is also of importance for all of us who use the
Internet to have accurate information about it's development from reliable
sources.
Wil Bigelow
- - - - - - - - - - - - - - - - - - -


} X-Sender: galler-at-g.imap.itd.umich.edu (Unverified)
} Mime-Version: 1.0
} Date: Sat, 21 Oct 2000 11:00:26 -0400
} To: coe-fac-staff-at-umich.edu
} From: "Bernard A. Galler" {galler-at-umich.edu}
} Subject: Setting the record straight ...
} Status:
}
} Many of us recently saw the excellent statement by Vint Cerf and Bob Kahn
} on Gore's "Inventing the Internet" claim (appended below). I asked for and
} received permission from Vint to send it to our local paper, the Ann Arbor
} News. When I sent it to them, I added a preface which said: "I am
} submitting this important Letter to the Editor, with permission. It was
} written by two people who are generally regarded in the computer industry
} as the real inventors of the Internet."
}
} Unfortunately, they did publish "my letter" without my preface and without
} the first half of the Cerf/Kahn statement, but with my name at the bottom.
} Not only did they remove the validation of the statement, since there is no
} longer any mention of the real authors, but they cast me in the role of
} plagiarist. I have received several email messages and phone calls
} congratulating me on my letter and my excellent writing!
}
} You may be sure that I will visit the Ann Arbor News when I return to Ann
} Arbor on Monday.
} Bernie
} ===================================================================
} Al Gore and the Internet
}
} By Robert Kahn and Vinton Cerf
} Al Gore was the first political leader to recognize the importance of the
} Internet and to promote and support its development.
}
} No one person or even small group of persons exclusively "invented" the
} Internet. It is the result of many years of ongoing collaboration among
} people in government and the university community. But as the two people
} who designed the basic architecture and the core protocols that make the
} Internet work, we would like to acknowledge VP Gore's contributions as a
} Congressman, Senator and as Vice President. No other elected official, to
} our knowledge, has made a greater contribution over a longer period of time.
}
} Last year the Vice President made a straightforward statement on his
} role. He said: "During my service in the United States Congress I took the
} initiative in creating the Internet." We don't think, as some people have
} argued, that Gore intended to claim he "invented" the Internet. Moreover,
} there is no question in our minds that while serving as Senator, Gore's
} initiatives had a significant and beneficial effect on the still-evolving
} Internet. The fact of the matter is that Gore was talking about and
} promoting the Internet long before most people were listening. We feel it
} is timely to offer our perspective.
}
} As far back as the 1970s Congressman Gore promoted the idea of high speed
} telecommunications as an engine for both economic growth and the
} improvement of our educational system. He was the first elected official
} to grasp the potential of computer communications to have a broader impact
} than just improving the conduct of science and scholarship. Though easily
} forgotten, now, at the time this was an unproven and controversial
} concept. Our work on the Internet started in 1973 and was based on even
} earlier work that took place in the mid-late 1960s. But the Internet, as we
} know it today, was not deployed until 1983. When the Internet was still in
} the early stages of its deployment, Congressman Gore provided intellectual
} leadership by helping create the vision of the potential benefits of high
} speed computing and communication. As an example, he sponsored hearings on
} how advanced technologies might be put to use in areas like coordinating
} the response of government agencies to natural disasters and other crises.
}
} As a Senator in the 1980s Gore urged government agencies to consolidate
} what at the time were several dozen different and unconnected networks into
} an "Interagency Network." Working in a bi-partisan manner with officials
} in Ronald Reagan and George Bush's administrations, Gore secured the
} passage of the High Performance Computing and Communications Act in
} 1991. This "Gore Act" supported the National Research and Education
} Network (NREN) initiative that became one of the major vehicles for the
} spread of the Internet beyond the field of computer science.
}
} As Vice President Gore promoted building the Internet both up and out, as
} well as releasing the Internet from the control of the government agencies
} that spawned it. He served as the major administration proponent for
} continued investment in advanced computing and networking and private
} sector initiatives such as Net Day. He was and is a strong proponent of
} extending access to the network to schools and libraries. Today,
} approximately 95% of our nation's schools are on the Internet. Gore
} provided much-needed political support for the speedy privatization of the
} Internet when the time arrived for it to become a commercially-driven
} operation.
}
} There are many factors that have contributed to the Internet's rapid growth
} since the later 1980s, not the least of which has been political support
} for its privatization and continued support for research in advanced
} networking technology. No one in public life has been more intellectually
} engaged in helping to create the climate for a thriving Internet than the
} Vice President. Gore has been a clear champion of this effort, both in the
} councils of government and with the public at large.
}
} The Vice President deserves credit for his early recognition of the value
} of high speed computing and communication and for his long-term and
} consistent articulation of the potential value of the Internet to American
} citizens and industry and, indeed, to the rest of the world.
}
}
} Bernard A. Galler
} E-mail: galler-at-umich.edu
} Fax: 734-668-9998
}

Wilbur C. Bigelow, Prof. Emeritus
Materials Sci. & Engr., University of Michigan
Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu;
Fx:734-763-4788; Ph:734-662-5237





From daemon Mon Oct 23 15:38:15 2000



From: beth-at-dogwood.botany.uga.edu (Beth Richardson)
Date: Mon, 23 Oct 2000 18:14:03 -0500
Subject: photoshop & PEI

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Photoshop is used for all of the things mentioned, and that only scratches
the surface of what is possible.
In addition to the full blown graphics aspects, the current full versions
of Photoshop (version 5.5 with 6.0 set to release any day now) include PDF
support
and Adobe ImageReady, an application for preparing images for web viewing.
If you require basic image manipulation tools and Text capabilities,
consider
PhotoShop LE. Its $99 and has the most commonly used features of its big
brother.
A comparison of "LE" vs 6.0 is available
at : http://www.adobe.com/products/photoshople/comparison.html

George Laing
National Graphic Supply




Bill,
It's not from the microscopist's point of view but there's an article in a
magazine called Photo Electronic Imaging (PEI) that may help answer some of
your questions about Photoshop. I really enjoy this magazine. The October
issue gives lots of good info on Photoshop 6 (pages 16-17 show a 6.0
feature roundup). And, every month there's an article called Photoshop -
Instant Expert by Ben Willmore. The web site is www.peimag.com.

Beth

**************************************
Beth Richardson
EM Lab Coordinator
Botany Department
University of Georgia
Athens, GA 30602

Phone - (706) 542-1790
FAX - (706) 542-1805
Email - beth-at-dogwood.botany.uga.edu

"Between the two evils,
I always pick the one I never tried before". Mae West (1893-1980)
**************************************





From daemon Mon Oct 23 17:21:31 2000



From: Richard Leapman :      leapman-at-helix.nih.gov
Date: Mon, 23 Oct 2000 18:21:38 -0400
Subject: New position available at NIH

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


*** POSITION AVAILABLE ***

NATIONAL INSTITUTES OF HEALTH
Bethesda, Maryland
Division of Bioengineering & Physical Science
Supramolecular Structure & Function Lab.

Physical /life scientist (Biologist GS9/GS11/GS12) at BS or MS level with
solid technical experience in thin-section transmission electron
microscopy. Experience in advanced techniques in analytical electron
microscopy and structural biology (cryosectioning, high-pressure freezing,
and macromolecular preparation methods) is desirable, although on-the-job
training is possible for an experienced and meticulous microscopist. PROOF
OF U.S. CITIZENSHIP IS REQUIRED for this appointment.


For further information please contact:

Dr. Richard Leapman
Division of Bioengineering & Physical Science
Bldg. 13, Rm. 3N17
National Institutes of Health
Bethesda, MD 20892
Tel: (301) 496-2599
FAX: (301) 496-6608
E-mail: leapman-at-helix.nih.gov


Applications (with curriculum vitae or federal employment form SF-171)
should include the reference number ORS-00-0231, and should be post marked
by November 7, 2000 and sent to:

Ms. Karen Harris
National Institutes of Health
ORS Human Resources Office
31 Center Drive Msc 2157
Bldg 31, Rm 4B41
Bethesda, MD 20892

E-mail: orspersonnel-at-mail.nih.gov
Phone: 301-496-5623
FAX: 301-402-1057

Detailed information about the position is available at

http://careerhere.nih.gov/CHPublic/HRShowVac.taf?&VACANCY_uid1=5717








From daemon Mon Oct 23 17:55:14 2000



From: Ruth Yamawaki :      Ryamawaki-at-cmexchange.stanford.edu
Date: Mon, 23 Oct 2000 15:52:51 -0700
Subject: Uranyl Nitrate

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Our health and safety officer wants to know if Uranyl Nitrate is used as an
EM stain. Does anyone use Uranyl Nitrate? He did not tell me why he needs
to know.

Thanks,

Ruth

***************************************
Ruth Yamawaki
Department of Comparative Medicine
Stanford University
Stanford, CA 94305
***************************************





From daemon Mon Oct 23 18:38:21 2000



From: Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 23 Oct 2000 16:36:03 -0700
Subject: Re: Setting the record straight ...

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 12:05 PM 10/23/00, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Amazing and informative. I truly thought that the Internet was created
by DARPA and BBN. I had no idea that Gore played such a pivotal
role in its creation. When I was working with DARPA in the '70s, Gore's
name never came up. I suppose it was because the lower level creators
were just computer scientists and engineers.

Thanks for the update.

gary g.




From daemon Mon Oct 23 18:42:42 2000



From: hma-at-tupphysiol1.bp.dal.ca ()
Date: Mon, 23 Oct 2000 18:40:23 -0500
Subject: BrdU staining Question:

Contents Retrieved from Microscopy Listserver Archives
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Email: hma-at-tupphysiol1.bp.dal.ca
Name: HuaLong Ma
School: Dalhousie University

Question: I am reading a paper using BrdU staining the cell and observe the
fluorescence micrograph and confocal micrograph, could you give some
explainations about these techniques and what is special about them. By the
way, what is " Camera lucida" ? thanks


---------------------------------------------------------------------------





From daemon Mon Oct 23 19:32:01 2000



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Mon, 23 Oct 2000 17:21:43 -0700
Subject: Fwd: Setting the record straight ...

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I don't believe that one person may claim that he or she has
invented/created the Internet. Internet is a result of join efforts of
many people from many countries (for this reason it called INTERnet). As my
knowledge, Internet was grew as a fast way to communicate between members
of the scientific community at the beginning. Because of international
nature of the scientific community, Internet was "INTER" from the very
early steps. I have no idea how Al Gore's was involved in such spontaneous
out of control process. Moreover, I do believe, that the beauty of the
Internet is their "deregulation" and non-controlling by any
government-related agency. This is my personal opinion.

Sergey

} Date: Mon, 23 Oct 2000 15:05:17 -0400
} From: Wil Bigelow {bigelow-at-engin.umich.edu}
} Subject: Setting the record straight ...
} X-Sender: bigelow-at-srvr5.engin.umich.edu
} To: Microscopy Listserver {microscopy-at-sparc5.microscopy.com}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu






From daemon Mon Oct 23 22:49:44 2000



From: Robert Ruscica :      ruscica-at-etp-usa.com
Date: Mon, 23 Oct 2000 21:44:47 -0700
Subject: Re: Setting the record straight ...

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Wil,
With all due respect, no more political mesages.
Bob Ruscica

Wil Bigelow wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hi Listers:
} Since all of us are heavily involved with the use of the internet, I
} thought this message I just received from Prof. Galler, one of the true
} computer experts here at the Univ. of Michigan, and one of the poeople who
} has been deeply involved in the development of our computer system, might
} be of interest to you. It not only sheds light on some of the political
} aspects of the development of the internet, but also shows how risky it can
} be to deal with news media.
}
} I know this has some political overtones (and perhaps undertones, too)
} However, I think it is also of importance for all of us who use the
} Internet to have accurate information about it's development from reliable
} sources.
} Wil Bigelow
} - - - - - - - - - - - - - - - - - - -
}
} } X-Sender: galler-at-g.imap.itd.umich.edu (Unverified)
} } Mime-Version: 1.0
} } Date: Sat, 21 Oct 2000 11:00:26 -0400
} } To: coe-fac-staff-at-umich.edu
} } From: "Bernard A. Galler" {galler-at-umich.edu}
} } Subject: Setting the record straight ...
} } Status:
} }
} } Many of us recently saw the excellent statement by Vint Cerf and Bob Kahn
} } on Gore's "Inventing the Internet" claim (appended below). I asked for and
} } received permission from Vint to send it to our local paper, the Ann Arbor
} } News. When I sent it to them, I added a preface which said: "I am
} } submitting this important Letter to the Editor, with permission. It was
} } written by two people who are generally regarded in the computer industry
} } as the real inventors of the Internet."
} }
} } Unfortunately, they did publish "my letter" without my preface and without
} } the first half of the Cerf/Kahn statement, but with my name at the bottom.
} } Not only did they remove the validation of the statement, since there is no
} } longer any mention of the real authors, but they cast me in the role of
} } plagiarist. I have received several email messages and phone calls
} } congratulating me on my letter and my excellent writing!
} }
} } You may be sure that I will visit the Ann Arbor News when I return to Ann
} } Arbor on Monday.
} } Bernie
} } ===================================================================
} } Al Gore and the Internet
} }
} } By Robert Kahn and Vinton Cerf
} } Al Gore was the first political leader to recognize the importance of the
} } Internet and to promote and support its development.
} }
} } No one person or even small group of persons exclusively "invented" the
} } Internet. It is the result of many years of ongoing collaboration among
} } people in government and the university community. But as the two people
} } who designed the basic architecture and the core protocols that make the
} } Internet work, we would like to acknowledge VP Gore's contributions as a
} } Congressman, Senator and as Vice President. No other elected official, to
} } our knowledge, has made a greater contribution over a longer period of time.
} }
} } Last year the Vice President made a straightforward statement on his
} } role. He said: "During my service in the United States Congress I took the
} } initiative in creating the Internet." We don't think, as some people have
} } argued, that Gore intended to claim he "invented" the Internet. Moreover,
} } there is no question in our minds that while serving as Senator, Gore's
} } initiatives had a significant and beneficial effect on the still-evolving
} } Internet. The fact of the matter is that Gore was talking about and
} } promoting the Internet long before most people were listening. We feel it
} } is timely to offer our perspective.
} }
} } As far back as the 1970s Congressman Gore promoted the idea of high speed
} } telecommunications as an engine for both economic growth and the
} } improvement of our educational system. He was the first elected official
} } to grasp the potential of computer communications to have a broader impact
} } than just improving the conduct of science and scholarship. Though easily
} } forgotten, now, at the time this was an unproven and controversial
} } concept. Our work on the Internet started in 1973 and was based on even
} } earlier work that took place in the mid-late 1960s. But the Internet, as we
} } know it today, was not deployed until 1983. When the Internet was still in
} } the early stages of its deployment, Congressman Gore provided intellectual
} } leadership by helping create the vision of the potential benefits of high
} } speed computing and communication. As an example, he sponsored hearings on
} } how advanced technologies might be put to use in areas like coordinating
} } the response of government agencies to natural disasters and other crises.
} }
} } As a Senator in the 1980s Gore urged government agencies to consolidate
} } what at the time were several dozen different and unconnected networks into
} } an "Interagency Network." Working in a bi-partisan manner with officials
} } in Ronald Reagan and George Bush's administrations, Gore secured the
} } passage of the High Performance Computing and Communications Act in
} } 1991. This "Gore Act" supported the National Research and Education
} } Network (NREN) initiative that became one of the major vehicles for the
} } spread of the Internet beyond the field of computer science.
} }
} } As Vice President Gore promoted building the Internet both up and out, as
} } well as releasing the Internet from the control of the government agencies
} } that spawned it. He served as the major administration proponent for
} } continued investment in advanced computing and networking and private
} } sector initiatives such as Net Day. He was and is a strong proponent of
} } extending access to the network to schools and libraries. Today,
} } approximately 95% of our nation's schools are on the Internet. Gore
} } provided much-needed political support for the speedy privatization of the
} } Internet when the time arrived for it to become a commercially-driven
} } operation.
} }
} } There are many factors that have contributed to the Internet's rapid growth
} } since the later 1980s, not the least of which has been political support
} } for its privatization and continued support for research in advanced
} } networking technology. No one in public life has been more intellectually
} } engaged in helping to create the climate for a thriving Internet than the
} } Vice President. Gore has been a clear champion of this effort, both in the
} } councils of government and with the public at large.
} }
} } The Vice President deserves credit for his early recognition of the value
} } of high speed computing and communication and for his long-term and
} } consistent articulation of the potential value of the Internet to American
} } citizens and industry and, indeed, to the rest of the world.
} }
} }
} } Bernard A. Galler
} } E-mail: galler-at-umich.edu
} } Fax: 734-668-9998
} }
}
} Wilbur C. Bigelow, Prof. Emeritus
} Materials Sci. & Engr., University of Michigan
} Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu;
} Fx:734-763-4788; Ph:734-662-5237



From daemon Tue Oct 24 03:29:31 2000



From: Su Sajip :      SSajip-at-iqep.com
Date: Tue, 24 Oct 2000 09:19:15 +0100
Subject: Re: Contact info for Cambridge Image Technology Cathodoluminesce

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-----------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


}
} We are trying to track down the location of "Cambridge Image
} Technology," manufacturers of cathodoluminescence stages for
} optical microscopes. Can anyone help? Thanks in advance,
}
} Mati


Here is a link to the web page for Cambridge Image Technology Ltd.

http://ds.dial.pipex.com/town/park/xes84/prod01.htm


Hope this helps!

Cheers,

Su



_____________________________
Dr Su Sajip
Characterisation Development Engineer
IQE (Europe) Ltd.
United Kingdom
Cypress Drive, St. Mellons
Cardiff CF3 OEG * Wales, UK

http://www.iqep.com
Tel: +(44) 2920 839-436
Fax: +(44) 2920 839-401






From daemon Tue Oct 24 04:00:06 2000



From: Richard Beanland +44 1327 356363 :      richard.beanland-at-marconi.com
Date: Tue, 24 Oct 2000 09:41:34 +0000 (GMT)
Subject: Re: PhotoShop usage

Contents Retrieved from Microscopy Listserver Archives
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Bill,
I use PhotoShop for:
Brightness/contrast/gamma/colour balance adjustments
Re-size, rotate and annotate
Select colour channels from RGB images
Background correction
Distortion of images to show make small thickness variations more visible
Fiducial markers (very handy, I have a marker for each mag of my optical microscopes, SEM and TEM)
Getting rid of blemishes/unwanted features (should I own up to this?)
Making false colour SEM images
Making movie sequences
And probably a few more things I can't think of at the moment.
I've used it to process } 10,000 images over the last 3 years.

I would only add that although it is expensive when bought by itself, it can often be obtained much more cheaply as part of a package for a scanner or camera.

Richard


} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I was talking to a friend of mine this morning about what software he
} should have to do digital imaging. Reflexively I said PhotoShop and was
} asked how he would use it. I told him the standard things - contrast
} enhancement, color matching across different media, annotation, resizing,
} etc. His response was that for the price was that all it did (a little
} naivety but a fair question) . You know, sometimes you get in a rut and it
} set me to wondering how other people are using PhotoShop? It's not like
} I'm selling the software but I'd like to be able to give a better answer
} before telling someone they should plunk down that much money. Aside from
} image analysis plug-ins (which I had forgotten about when we were talking)
} how else is PhotoShop being used - anything fancy to justify the cost?
}
} Bill Miller
}

==============================================================
Richard Beanland,
Structural Analysis Lab,
Caswell Technology,
Caswell,
Towcester,
Northants NN12 8EQ

e-mail richard.beanland-at-marconi.com
Tel. +44 1327 356363
Fax. +44 1327 356398
==============================================================
"The information contained in this message is legally privileged and confidential information intended for the eyes of the individual or entity named above. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message is strictly prohibited. If you have received this message in error, please notify us immediately by telephone.
Caswell Technology is the trading name of Marconi Caswell Limited. Registered in London No. 3694360 Registered Office: One Bruton Street London W1X 8AQ. Holding Company: Marconi plc."







From daemon Tue Oct 24 04:35:22 2000



From: Massimo Catalano :      massimo.catalano-at-ime.le.cnr.it
Date: Tue, 24 Oct 2000 11:31:33 +0200
Subject: 5th Multinational Congress on Electron Microscopy - Lecce,

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Colleagues,

this message is to advertise the 5th multinational congress on electron
microscopy, that will be held in Lecce (Italy) from September 20 to
September 25, 2001.

The Congress is organized by the microscopy societies of the following
countries:

Austria
Croatia
Czech Rep
Hungary
Italy
Slovak Rep
Slovenia

The meeting is a forum for all scientists active in all fields of microscopy.

The topics covered will be:

Architecture of the nucleus and nuclear import and export, art,
biomaterials, biosensors, environmental microscopy, cell and cell
components, cell death in biology and pathology, chemical analysis and
mapping, microscopy in human health, microscopy of nanostructural
materials, microscopy of structural and functional materials,
energy-filtered electron microscopy, instrumental development,
investigations of thin films for sensor technology, microbiology,
microscopy of semiconducting materials, parassitology, plant cell biolgy,
remote microscopy, strain analysis, structure biology, structural
determination and refining, viruses, teleoperation and computer assisted
operation, nanotechnology, single molecule microscopy and manipulation

A large exhibition area will be available to manufacturers. Open labs and
tutorials will be organized.

Information can be found at www.mcem5.unile.it, where brochures can be
downloaded too, or directly writing to me (I am president of the congress
(materials science section) together with Prof. L. Dini, (biomedical field)).

We espect and hope to have a large participation from all around the world.
Hope to see you all in Lecce.

Thanks

Massimo

Dr. Massimo Catalano
IME-CNR
Via Arnesano
73100 Lecce -ITALY
ph: +39 0832 322362
fax: +39 0832 325299
email: massimo.catalano-at-ime.le.cnr.it
www.mcem5.unile.it
www.ime.le.cnr.it
www.ime.le.cnr.it/sime/sime.htm




From daemon Tue Oct 24 05:09:38 2000



From: Massimo Catalano :      massimo.catalano-at-ime.le.cnr.it
Date: Tue, 24 Oct 2000 12:06:53 +0200
Subject: Re: Setting the record straight ...

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listservers,

with all due respect to anyone, and keeping in mind that this listserver is
mailny populated by Americans (so I belong to a minority, here, being
Italian) I would like to espress my opinion on Robert Ruscica's message.

I strongly believe that internet is one of the few places where a human
being can "still" express his own opinion on something.

Expressing an idea, reporting an opinion, is a way to express his own
"freedom" and freedom is important to anyone.

Provided that anyone has the possibility of replying a message, of
expressing a different opinion, of stating what he thinks too, I find that
"discussing" in a positive way is always stimulating, and a high level
auditorium, as this listserver is supposed to be, is a good place to
discuss, as science and research are (unfortunately or fortunately)
strictly related to politics.

Of course this is just my opinion.

Best regards to all

Massimo




From daemon Tue Oct 24 06:39:36 2000



From: Chuck Butterick :      cbutte-at-ameripol.com
Date: 10/23/00 3:05 PM
Subject: Setting the record straight ...

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




} Email: hma-at-tupphysiol1.bp.dal.ca
} Name: HuaLong Ma
} School: Dalhousie University
}
} Question: I am reading a paper using BrdU staining the cell and observe
the
} fluorescence micrograph and confocal micrograph, could you give some
} explainations about these techniques and what is special about them. By
the
} way, what is " Camera lucida" ? thanks
}

HuaLong -

I can't help you much with your flourescence and confocal questions, but I
do know what a Camera lucida is - it's a simple optical device which allows
you to "project" and enlarge the image seen in a microscope onto a piece of
paper, so that you can then draw it more accurately. It may be as simple as
a tiltable mirror mounted on a pole with a base which also tilts, allowing
the user to make whatever adjustments necessary to optimize the
"projection".
These devices have been around for a long time, (at least a hundred years,
and probably much longer) and were often used in the original production of
some of the fine, meticulous drawings one sees in antique texts in a wide
variety of subjects. I wouldn't be surprised if some are still in use in
anatomical and paleontological studies - sometimes a hand drawing is the
best way to illustrate a certain feature (and since you need fingers to
hold a pencil, such drawings may have been the first "digital" micrographs
(sorry)). In my own former specialty, micropaleontology, Camera lucida's
are still being used now and then, and they may even still be commercially
available.
I see you're at Dalhousie; if you ever want to have a look at one, I'd be
pleased to show you ours (after wiping the dust off it); we're just across
the bridge.

Frank Thomas
MicroAnalysis Facility
Geological Survey of Canada (Atlantic)
Bedford Institute of Oceanography
Dartmouth, Nova Scotia
Canada
B2Y 4A2

(902) 426-4635


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Let me join on the bandwagon....unless we want to start posting "Bush for
President" signs on this listserv, we ought to drop the clever, some might
even say "subliminal", political agendas.

SethG



Wil,
With all due respect, no more political mesages.
Bob Ruscica

Wil Bigelow wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hi Listers:
} Since all of us are heavily involved with the use of the internet, I
} thought this message I just received from Prof. Galler, one of the true
} computer experts here at the Univ. of Michigan, and one of the poeople who
} has been deeply involved in the development of our computer system, might
} be of interest to you. It not only sheds light on some of the political
} aspects of the development of the internet, but also shows how risky it
can
} be to deal with news media.
}
} I know this has some political overtones (and perhaps undertones, too)
} However, I think it is also of importance for all of us who use the
} Internet to have accurate information about it's development from
reliable
} sources.
} Wil Bigelow
} - - - - - - - - - - - - - - - - - - -
}
} } X-Sender: galler-at-g.imap.itd.umich.edu (Unverified)
} } Mime-Version: 1.0
} } Date: Sat, 21 Oct 2000 11:00:26 -0400
} } To: coe-fac-staff-at-umich.edu
} } From: "Bernard A. Galler" {galler-at-umich.edu}
} } Subject: Setting the record straight ...
} } Status:
} }
} } Many of us recently saw the excellent statement by Vint Cerf and Bob Kahn
} } on Gore's "Inventing the Internet" claim (appended below). I asked for
and
} } received permission from Vint to send it to our local paper, the Ann
Arbor
} } News. When I sent it to them, I added a preface which said: "I am
} } submitting this important Letter to the Editor, with permission. It was
} } written by two people who are generally regarded in the computer industry
} } as the real inventors of the Internet."
} }
} } Unfortunately, they did publish "my letter" without my preface and
without
} } the first half of the Cerf/Kahn statement, but with my name at the
bottom.
} } Not only did they remove the validation of the statement, since there is
no
} } longer any mention of the real authors, but they cast me in the role of
} } plagiarist. I have received several email messages and phone calls
} } congratulating me on my letter and my excellent writing!
} }
} } You may be sure that I will visit the Ann Arbor News when I return to Ann
} } Arbor on Monday.
} } Bernie
} } ===================================================================
} } Al Gore and the Internet
} }
} } By Robert Kahn and Vinton Cerf
} } Al Gore was the first political leader to recognize the importance of the
} } Internet and to promote and support its development.
} }
} } No one person or even small group of persons exclusively "invented" the
} } Internet. It is the result of many years of ongoing collaboration among
} } people in government and the university community. But as the two people
} } who designed the basic architecture and the core protocols that make the
} } Internet work, we would like to acknowledge VP Gore's contributions as a
} } Congressman, Senator and as Vice President. No other elected official, to
} } our knowledge, has made a greater contribution over a longer period of
time.
} }
} } Last year the Vice President made a straightforward statement on his
} } role. He said: "During my service in the United States Congress I took
the
} } initiative in creating the Internet." We don't think, as some people have
} } argued, that Gore intended to claim he "invented" the Internet. Moreover,
} } there is no question in our minds that while serving as Senator, Gore's
} } initiatives had a significant and beneficial effect on the still-evolving
} } Internet. The fact of the matter is that Gore was talking about and
} } promoting the Internet long before most people were listening. We feel it
} } is timely to offer our perspective.
} }
} } As far back as the 1970s Congressman Gore promoted the idea of high speed
} } telecommunications as an engine for both economic growth and the
} } improvement of our educational system. He was the first elected official
} } to grasp the potential of computer communications to have a broader
impact
} } than just improving the conduct of science and scholarship. Though easily
} } forgotten, now, at the time this was an unproven and controversial
} } concept. Our work on the Internet started in 1973 and was based on even
} } earlier work that took place in the mid-late 1960s. But the Internet, as
we
} } know it today, was not deployed until 1983. When the Internet was still
in
} } the early stages of its deployment, Congressman Gore provided
intellectual
} } leadership by helping create the vision of the potential benefits of high
} } speed computing and communication. As an example, he sponsored hearings
on
} } how advanced technologies might be put to use in areas like coordinating
} } the response of government agencies to natural disasters and other
crises.
} }
} } As a Senator in the 1980s Gore urged government agencies to consolidate
} } what at the time were several dozen different and unconnected networks
into
} } an "Interagency Network." Working in a bi-partisan manner with officials
} } in Ronald Reagan and George Bush's administrations, Gore secured the
} } passage of the High Performance Computing and Communications Act in
} } 1991. This "Gore Act" supported the National Research and Education
} } Network (NREN) initiative that became one of the major vehicles for the
} } spread of the Internet beyond the field of computer science.
} }
} } As Vice President Gore promoted building the Internet both up and out, as
} } well as releasing the Internet from the control of the government
agencies
} } that spawned it. He served as the major administration proponent for
} } continued investment in advanced computing and networking and private
} } sector initiatives such as Net Day. He was and is a strong proponent of
} } extending access to the network to schools and libraries. Today,
} } approximately 95% of our nation's schools are on the Internet. Gore
} } provided much-needed political support for the speedy privatization of
the
} } Internet when the time arrived for it to become a commercially-driven
} } operation.
} }
} } There are many factors that have contributed to the Internet's rapid
growth
} } since the later 1980s, not the least of which has been political support
} } for its privatization and continued support for research in advanced
} } networking technology. No one in public life has been more intellectually
} } engaged in helping to create the climate for a thriving Internet than the
} } Vice President. Gore has been a clear champion of this effort, both in
the
} } councils of government and with the public at large.
} }
} } The Vice President deserves credit for his early recognition of the value
} } of high speed computing and communication and for his long-term and
} } consistent articulation of the potential value of the Internet to
American
} } citizens and industry and, indeed, to the rest of the world.
} }
} }
} } Bernard A. Galler
} } E-mail: galler-at-umich.edu
} } Fax: 734-668-9998
} }
}
} Wilbur C. Bigelow, Prof. Emeritus
} Materials Sci. & Engr., University of Michigan
} Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu;
} Fx:734-763-4788; Ph:734-662-5237




From root Tue Oct 24 08:18:14 2000
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Yes, Wil's posting definitely has political overtones. So does the
timing of the posting just 2 weeks before a presidential election.
Are we now going to be inundated by apologists for every outrageous
claim made by the Democrat candidate? I don't think I'd have time to
read all email displaying torturous mental gymnastics justifying all
the prevarications. Save it for the ballot box and keep it off the
listserver.

Chuck Butterick



______________________________ Reply Separator _________________________________


------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Hi Listers:
Since all of us are heavily involved with the use of the internet, I
thought this message I just received from Prof. Galler, one of the true
computer experts here at the Univ. of Michigan, and one of the poeople who
has been deeply involved in the development of our computer system, might
be of interest to you. It not only sheds light on some of the political
aspects of the development of the internet, but also shows how risky it can
be to deal with news media.

I know this has some political overtones (and perhaps undertones, too)
However, I think it is also of importance for all of us who use the
Internet to have accurate information about it's development from reliable
sources.
Wil Bigelow
- - - - - - - - - - - - - - - - - - -


} X-Sender: galler-at-g.imap.itd.umich.edu (Unverified)
} Mime-Version: 1.0
} Date: Sat, 21 Oct 2000 11:00:26 -0400
} To: coe-fac-staff-at-umich.edu
} From: "Bernard A. Galler" {galler-at-umich.edu}
} Subject: Setting the record straight ...
} Status:
}
} Many of us recently saw the excellent statement by Vint Cerf and Bob Kahn
} on Gore's "Inventing the Internet" claim (appended below). I asked for and
} received permission from Vint to send it to our local paper, the Ann Arbor
} News. When I sent it to them, I added a preface which said: "I am
} submitting this important Letter to the Editor, with permission. It was
} written by two people who are generally regarded in the computer industry
} as the real inventors of the Internet."
}
} Unfortunately, they did publish "my letter" without my preface and without
} the first half of the Cerf/Kahn statement, but with my name at the bottom.
} Not only did they remove the validation of the statement, since there is no
} longer any mention of the real authors, but they cast me in the role of
} plagiarist. I have received several email messages and phone calls
} congratulating me on my letter and my excellent writing!
}
} You may be sure that I will visit the Ann Arbor News when I return to Ann
} Arbor on Monday.
} Bernie
} ===================================================================
} Al Gore and the Internet
}
} By Robert Kahn and Vinton Cerf
} Al Gore was the first political leader to recognize the importance of the
} Internet and to promote and support its development.
}
} No one person or even small group of persons exclusively "invented" the
} Internet. It is the result of many years of ongoing collaboration among
} people in government and the university community. But as the two people
} who designed the basic architecture and the core protocols that make the
} Internet work, we would like to acknowledge VP Gore's contributions as a
} Congressman, Senator and as Vice President. No other elected official, to
} our knowledge, has made a greater contribution over a longer period of time.
}
} Last year the Vice President made a straightforward statement on his } role.
He said: "During my service in the United States Congress I took the
} initiative in creating the Internet." We don't think, as some people have
} argued, that Gore intended to claim he "invented" the Internet. Moreover,
} there is no question in our minds that while serving as Senator, Gore's
} initiatives had a significant and beneficial effect on the still-evolving
} Internet. The fact of the matter is that Gore was talking about and
} promoting the Internet long before most people were listening. We feel it
} is timely to offer our perspective.
}
} As far back as the 1970s Congressman Gore promoted the idea of high speed
} telecommunications as an engine for both economic growth and the
} improvement of our educational system. He was the first elected official
} to grasp the potential of computer communications to have a broader impact
} than just improving the conduct of science and scholarship. Though easily
} forgotten, now, at the time this was an unproven and controversial
} concept. Our work on the Internet started in 1973 and was based on even
} earlier work that took place in the mid-late 1960s. But the Internet, as we
} know it today, was not deployed until 1983. When the Internet was still in
} the early stages of its deployment, Congressman Gore provided intellectual
} leadership by helping create the vision of the potential benefits of high
} speed computing and communication. As an example, he sponsored hearings on
} how advanced technologies might be put to use in areas like coordinating
} the response of government agencies to natural disasters and other crises.
}
} As a Senator in the 1980s Gore urged government agencies to consolidate
} what at the time were several dozen different and unconnected networks into
} an "Interagency Network." Working in a bi-partisan manner with officials
} in Ronald Reagan and George Bush's administrations, Gore secured the
} passage of the High Performance Computing and Communications Act in
} 1991. This "Gore Act" supported the National Research and Education
} Network (NREN) initiative that became one of the major vehicles for the
} spread of the Internet beyond the field of computer science.
}
} As Vice President Gore promoted building the Internet both up and out, as
} well as releasing the Internet from the control of the government agencies
} that spawned it. He served as the major administration proponent for
} continued investment in advanced computing and networking and private
} sector initiatives such as Net Day. He was and is a strong proponent of
} extending access to the network to schools and libraries. Today,
} approximately 95% of our nation's schools are on the Internet. Gore
} provided much-needed political support for the speedy privatization of the
} Internet when the time arrived for it to become a commercially-driven
} operation.
}
} There are many factors that have contributed to the Internet's rapid growth
} since the later 1980s, not the least of which has been political support
} for its privatization and continued support for research in advanced
} networking technology. No one in public life has been more intellectually
} engaged in helping to create the climate for a thriving Internet than the
} Vice President. Gore has been a clear champion of this effort, both in the
} councils of government and with the public at large.
}
} The Vice President deserves credit for his early recognition of the value
} of high speed computing and communication and for his long-term and
} consistent articulation of the potential value of the Internet to American
} citizens and industry and, indeed, to the rest of the world.
}
}
} Bernard A. Galler
} E-mail: galler-at-umich.edu
} Fax: 734-668-9998
}

Wilbur C. Bigelow, Prof. Emeritus
Materials Sci. & Engr., University of Michigan
Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu;
Fx:734-763-4788; Ph:734-662-5237








From daemon Tue Oct 24 08:18:20 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Tue, 24 Oct 2000 08:04:34 -0500
Subject: Administrivia: Stick to Microscopy & Microanalysis... Leave out

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Mime-Version: 1.0
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Colleagues....

I've already replied privately to a number of you but
before it start to grow let's nip this one in the bud.

Unless the topic is Microscopy
and Microanalysis related please keep tangential
commentary off-line. Comments about the Internet and
what any politican's may have or have not said has
no direct bearing on our agenda.

Nestor
Your Friendly Neighborhood SysOp





From daemon Tue Oct 24 08:18:20 2000



From: Jinguo Wang :      jqw11-at-psu.edu
Date: Tue, 24 Oct 2000 09:12:18 -0400
Subject: Ask-A-Microscopist: EDS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers:

I met a problem in our EDS system, we have a TX 1255 Nortan detector and
4pai system (DTSA software). The problem is even the input counts is high
to 2000 to 3000 and the dead time is below 30%, the output counts is less
than 200, and when the input is less than 300 and dead time is less than
30%, there is no out put. I checked the discriminator adjustment in the
pulse processor, it is almost there. How can I adjust the system?

Regards!

J.G. Wang






Jinguo Wang
Materials Characterization Laboratory
194 MRI Building
5-9285




From daemon Tue Oct 24 08:40:33 2000



From: W. L. Steffens :      bsteffen-at-arches.uga.edu
Date: Tue, 24 Oct 2000 09:36:32 -0400 (Eastern Daylight Time)
Subject: Re: Uranyl Nitrate

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



On Mon, 23 Oct 2000 15:52:51 -0700 Ruth Yamawaki
{Ryamawaki-at-cmexchange.stanford.edu} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
Ruth,

Uranyl nitrate is used to make Reynolds lead citrate, a widely used TEM
post stain for ultrathin tissue sections. In this formula, the lead
nitrate is chelated by citrate. Hope this is of some use


} Our health and safety officer wants to know if Uranyl Nitrate is used
} as an EM stain. Does anyone use Uranyl Nitrate? He did not tell me
} why he needs to know.
}

W. L. Steffens, Ph.D
University of Georgia




From daemon Tue Oct 24 09:10:00 2000



From: Gerard Gagne :      gerard.d.gagne-at-abbott.com
Date: Tue, 24 Oct 2000 09:09:10 -0500
Subject: Electron Microscopy/Histology Technician Position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am posting this on behalf or Dr. Richard Prum:

Electron Microscopy/Histology Technician

Full-time position available for an Electron Microscopy/Histology
Technician to work on a NSF funded research project on biological
structural colors. Responsibilities include specimen preparation,
observation, and imaging using transmission electron microscopy (TEM) and
light microscopy. Additional opportunties for active participation in
research and publication. Required qualifications: Bachelor's degree or
equivalent in an appropriate field; previous experience in microscopy or
histology; previous experience in laboratory research. Preferred
qualifications: previous experience in specimen preparation and sectioning
for TEM; previous experience operating transmission eletron microscopes;
previous experience in fixation, sectioning, and staining for light
microscope histology; previous experience in computer data base management;
graduate degree in an appropriate field. Salary: $25,000 per year
(full-time) with benefits. Send a CV, a written statement describing
previous experience and qualifications, and the names addresses, phone, and
email addresses of three professional references to: Dr. Richard O. Prum,
Natural History Museum, Dyche Hall, University of Kansas, Lawrence, KS
66045-2454; (785) 864-3897; prum-at-ukans.edu. Review of applications begins
Nov. 1, 2000 and will continue until position is filled. EO/AA Employer

------------------------------------------------------------------------------
--
Richard O. Prum
Associate Professor, Department of Ecology and Evolutionary Biology
Curator, Division of Ornithology
KU Natural History Museum
University of Kansas
Lawrence, KS 66045
Phone: (785)864-3897
Fax: (785)864-5335
Email: prum-at-ukans.edu
------------------------------------------------------------------------------
--



From daemon Tue Oct 24 09:48:32 2000



From: michael shaffer :      epmalab-at-darkwing.uoregon.edu
Date: Tue, 24 Oct 2000 07:32:12 -0700
Subject: RE: PhotoShop usage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Bill Miller writes ...

} ... I said PhotoShop, and was asked how he would use it.
} I told him the standard things - contrast
} enhancement, color matching across different media,
} annotation, resizing, etc. ...
} ...
} how else is PhotoShop being used - anything fancy to
} justify the cost?

We use PS5 here for annotation and presentation of spacial elemental
mapping (elemental x-ray maps), and average atomic number maps
(backscatter electron images). There isn't better software for
presenting this type of data in a "perceptually" uniform way. For
example, if you have a grayscale elemental map which represents SiO2
from 0% to 100%, with most softwares and typical monitors the change
from 20% to 30% isn't perceived the same as 70% to 80%, if perceived
at all. Opening and converting such an image into a Photoshop
gamma=2.2 working space makes this gradation perceptually uniform, and
much better for presentation.
The caveat here is the "conversion" when you do this ... that is, PS
makes the simple opening of the file such an innocent process, the
user may not notice his/her data has been modified. What was once a
pixel value of '128' is now '183'!! The data is now useless for
quantitative analysis. Make it a practice to keep (possibly even
write-protect) your original image files, and put your PS projects in
a separate directory.

cheerios, =shAf= :o)

{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {}
Michael Shaffer, R.A. - mshaf-at-darkwing.uoregon.edu
Geological Science's Electron Probe Facility - University of Oregon
http://epmalab.uoregon.edu/






From daemon Tue Oct 24 10:00:40 2000



From: Sara Miller :      saram-at-duke.edu
Date: Tue, 24 Oct 2000 10:39:44 -0400 (EDT)
Subject: Re: Uranyl Nitrate

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On Mon, 23 Oct 2000, Ruth Yamawaki wrote:

} Date: Mon, 23 Oct 2000 15:52:51 -0700
} From: Ruth Yamawaki {Ryamawaki-at-cmexchange.stanford.edu}
} To: "'Microscopy-at-sparc5.microscopy.com'"
{Microscopy-at-sparc5.microscopy.com}
} Subject: Uranyl Nitrate
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Our health and safety officer wants to know if Uranyl Nitrate is used as an
} EM stain. Does anyone use Uranyl Nitrate? He did not tell me why he needs
} to know.
}
} Thanks,
}
} Ruth
}
} ***************************************
} Ruth Yamawaki
} Department of Comparative Medicine
} Stanford University
} Stanford, CA 94305
} ***************************************
}
Uranyl nitrate, like uranyl acetate and other uranium salts, is used as a
stain in EM. The salt most frequently used is uranyl acetate because of
its staining efficiency. Uranium has a heavy atomic nucleus (92) and is
the heaviest metal used as a stain in EM. In tissue, it stabilizes
nucleic acids and membranes when used prior to embedding. It is
frequently used after sections are cut to "post stain" or further
increase the contrast of membranes, etc., in sections. Uranyl salts,
particularly acetate, are also used as negative stains for small things
such as viruses and small cellular organelles in suspension. The stain
darkens the background support and cracks and crevasses in the sample to
add contrast. Uranyl salt solutions should be handled with care because
they are radioactive. The alpha radiation is generally contained by
glass (lead not necessary), but nonetheless, one should not leave dried
pipets and droplets lying around to become airborne and possibly inhaled.

Hayat MA. Positive Staining for Electron Microscopy. 1975. Van Nostrand
Reinhold, NY.

Hayat MA, Miller SE. 1990. Negative Staining. McGraw-Hill, NY.



Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265




From daemon Tue Oct 24 10:03:08 2000



From: Charlie Cooney :      cbc-at-post.queensu.ca
Date: Tue, 24 Oct 2000 10:58:07 -0400
Subject: Heating Stages for Hitachi H600 TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I am currently trying to source an used heating holder, cooling holder and
a power supply for use in an Hitachi H600 TEM. If you have either of these
holders that are not in use and would like to sell them (or donate them to
a University) please contact me to see if we can come to a mutually
agreeable price.



Chas

Charlie Cooney
Metallographer
Dept. of Materials and Metallurgical Engineering
Queen's University
Nicol Hall
Kingston, Ontario
K7L 1N6


phone 613-533-2752
fax 613-533-6610
email cbc-at-post.queensu.ca



From daemon Tue Oct 24 10:21:36 2000



From: Jinguo Wang :      jqw11-at-psu.edu
Date: Tue, 24 Oct 2000 09:03:27 -0400
Subject: Enlarger

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers:

Can anyone provide me some information about a good enlarger suitable for
conventional TEM and HRTEM negatives? Your answer will be appreciated.

Regards!

J.G. Wang






Jinguo Wang
Materials Characterization Laboratory
194 MRI Building
5-9285




From daemon Tue Oct 24 10:35:57 2000



From: sghoshro-at-nmsu.edu
Date: Tue, 24 Oct 2000 09:33:34 -0600 (MDT)
Subject: Digital camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear colleagues,

We are looking into purchasing a digital microscope camera for our Zeiss
axioplan upright compound microscope. We are particularly interested in
color cameras that can capture good fluorescent images in addition to
brightfield images.

I would very much appreciate some suggestions regarding a good camera.

Thanks in advance.

Soumitra


*************************************************************
Soumitra Ghoshroy Ph.D.
Electron Microscopy Lab and Fluorescence Imaging Facility
Box 3EML
New Mexico State University
Las Cruces, NM 88003
Tel: 505-646-3600
Fax: 505-646-5665
e-mail:sghoshro-at-nmsu.edu
http://confocal.nmsu.edu/eml





From daemon Tue Oct 24 10:45:39 2000



From: William R. Oliver :      oliver-at-cpt.afip.org
Date: Tue, 24 Oct 2000 10:07:52 -0400 (EDT)
Subject: Re: Setting the record straight ...

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




On Tue, 24 Oct 2000, Massimo Catalano wrote:

}
} Dear Listservers,
}
} with all due respect to anyone, and keeping in mind that this listserver is
} mailny populated by Americans (so I belong to a minority, here, being
} Italian) I would like to espress my opinion on Robert Ruscica's message.
}
} I strongly believe that internet is one of the few places where a human
} being can "still" express his own opinion on something.
}
} Expressing an idea, reporting an opinion, is a way to express his own
} "freedom" and freedom is important to anyone.

Of course it is. Of course the internet is a wonderful thing. Of course
we all want to discuss our political philosophies (vote for Bush).

However, the internet is also a *big* thing. There are lots and lots
of people discussing lots and lots of different things here. Not
everyone needs to discuss all topics in every conversation.

Nader is an idiot.

It is not a restriction on any inalienable liberties for a group of
people to assemble and say "Here, today, we have chosen to talk about
microscopy. If you want to talk about Gore, feel free to go to the
political group. If you want to talk about woodworking, there is a
fine woodworking discussion group over there. If you want to talk
about Dungeons and Dragons, there is a great D&D group across the
hall." That part and parcel of the right of association, and it is
just as important as a right of speech.

Bush is wonderful.

It is further inappropriate for someone to come in and say "I don't care
what everyone has agreed to! I want to talk about Gore's policiy towards
D&D--playing woodworkers, and I don't care if it *is* a microscopy group.
*I* care about Gore and woodworking and you microscopists had just
better sit down and listen!"

Buchanan is a Nazi.

That is inappropriate, and it is not an abrogation of liberty for
folk to say "Well, I'd rather you didn't."

Gore is dangerous.

On a practical note, that is *why* we have mailing lists and news groups.
Do you suggest that we do away with all of them and just have one
large mailing list and one large newsgroup called misc.stuff? Of course
not.

Gore bad. Bush good.

In large part, we have managed to avoid direct moderation of most
newsgroups and mailing lists by agreeing to abide by self-selected
and agreed upon rules of behavior. Refusing to abide by these
choices will not result in greater "freedom," but will instead result
in the opposite -- the increasing direct moderation and censorship of
newsgroups as a last-ditch attempt to control spam and intrusion.

Bush in 2000.

billo -- still working on that subliminal thing.







From daemon Tue Oct 24 11:00:41 2000



From: Gib Ahlstrand :      giba-at-puccini.cdl.umn.edu
Date: Tue, 24 Oct 2000 11:08:35 -0500 (CDT)
Subject: Re: Uranyl Nitrate

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ruth,

M.A. Hayat mentions the use of uranyl nitrate on page 34 of his book Positive
Staining (c. 1975) as an electron stain. He says that it is soluble in water,
ethanol, acetone, and ether, but not in benzene, toluene and chloroform. The
aqueous solution can hold up to 56% of anhydrous salt (w/v) at 25 C. Uranyl
nitrate solutions are more stable than are uranyl acetate solutions but its less
efficient as a stain than the acetate.

I have never used it. Ask your saftey officer why he needs to know.

Gib Ahlstrand

} Responding to the message
} from Ruth Yamawaki {Ryamawaki-at-cmexchange.stanford.edu} :
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Our health and safety officer wants to know if Uranyl Nitrate is used as an
} EM stain. Does anyone use Uranyl Nitrate? He did not tell me why he needs
} to know.
}
} Thanks,
}
} Ruth
}
} ***************************************
} Ruth Yamawaki
} Department of Comparative Medicine
} Stanford University
} Stanford, CA 94305
} ***************************************

Gib Ahlstrand
Electron Optical Facility, University of Minnesota, Dept. Plant Pathology
495 Borlaug Hall, St. Paul, MN. USA. 55108 (612)625-8249
612-625-9728 FAX, giba-at-puccini.cdl.umn.edu
http://biosci.umn.edu/MIC/consortium.html




From daemon Tue Oct 24 11:02:07 2000



From: Mati Raudsepp :      raudsepp-at-unixg.ubc.ca
Date: Tue, 24 Oct 2000 08:59:59 -0700
Subject: Contact info for Cambridge Image Technology Cathodoluminescence

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Colleagues:

Thanks to all who replied; not only did we get a link for Cambridge, we
also received additional useful information.

Mati Raudsepp
Lori Kennedy
Greg Dipple

Mati Raudsepp, Associate Professor (Hon.)
Dept. of Earth & Ocean Sciences
6339 Stores Road
The University of British Columbia
Vancouver, BC V6T 1Z4

Tel: 604 822-6396
Fax: 604 822-6088




From daemon Tue Oct 24 11:32:12 2000



From: Timothy Schneider :      Timothy.Schneider-at-Mail.TJU.EDU
Date: Tue, 24 Oct 2000 12:17:17 -0400
Subject: RE: URANYL NITRATE

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


According to the Polysciences Catalog Uranyl Nitrate is a negative stain.
Also a photopolymerizing reagent for the polymerization of acrylamide gels
at low pH. I personally have no experience with the stuff, but would
imagine that it is similar to uranyl acetate which is a commonly used
negative or positive stain. Tim

Timothy G. Schneider
Director of Electron Microscopy
Department of Pathology
Room 229 Jefferson Hall
Thomas Jefferson University
1020 Locust St.
Philadelphia Pa. 19107
215-503-4798 work
610-613-8170 cellular
timothy.schneider-at-mail.tju.edu




From daemon Tue Oct 24 11:40:59 2000



From: Richard Beanland +44 1327 356363 :      richard.beanland-at-marconi.com
Date: Tue, 24 Oct 2000 17:37:32 +0000 (GMT)
Subject: D19b developer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All,
I am looking for a good replacement for D19b developer (for nuclear emulsion plates, as used in our X-ray radiography and topography images, no longer available). We are using Suprol at the moment, but would like something as good as the old stuff. Anyone have any ideas?

Richard

==============================================================
Richard Beanland,
Structural Analysis Lab,
Caswell Technology,
Caswell,
Towcester,
Northants NN12 8EQ

e-mail richard.beanland-at-marconi.com
Tel. +44 1327 356363
Fax. +44 1327 356398
==============================================================
"The information contained in this message is legally privileged and confidential information intended for the eyes of the individual or entity named above. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message is strictly prohibited. If you have received this message in error, please notify us immediately by telephone.
Caswell Technology is the trading name of Marconi Caswell Limited. Registered in London No. 3694360 Registered Office: One Bruton Street London W1X 8AQ. Holding Company: Marconi plc."







From daemon Tue Oct 24 11:41:28 2000



From: Michael Coviello :      coviello-at-mae.uta.edu
Date: Tue, 24 Oct 2000 11:47:41 -0500
Subject: EM-Sputter coater chamber glass

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all:
I am shopping around, looking for recommendation of glass suppliers for
that could make a chamber for our CRC-100 magnetron sputter coater. The
manufacturer wants alot for theirs and I am looking for alternative
suppliers that this community might be aware of . Any suggestions would
be greatly appreciated. Thanks in advance for your assistance.

Regards,
Mike Coviello
Lab Manager
Unversity of Texas -at- Arlington




From daemon Tue Oct 24 12:20:47 2000



From: Su, Phy-Huynh :      psu-at-shctampa.usf.edu
Date: Tue, 24 Oct 2000 13:03:33 -0400
Subject: FW: Easy way to help a good cause TOMORROW

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




} -----Original Message-----
} From: Berryhill, Bridgette
} Sent: Tuesday, October 24, 2000 10:15 AM
} To: Azizan, Azliyati; Barber, Janet; Chen, Lan; Gahagan, Jackie;
} Govindraj, Prasanthi; Grimm, David; Hall,Laura; Hernandez, Dan; Howell,
} Troy; Ingrassia, Angela; Kane, Brad; McKinnon,William; McPherson,
} Charlene; Su, Phy-Huynh; Vallet, Noelle; Westling, Jennifer; 'David
} Alexandrou'; 'Elyse Drossman'; 'Jane Dunlevy'; 'MaryBeth Colter'; 'Stacey
} Kennedy'
} Subject: FW: Easy way to help a good cause TOMORROW
}
}
} From: Amy Wolfson
} Sent: Monday, October 23, 2000 9:59 PM
} To: Debby Greenberg; Marilyn Weisman; Michele Schointuch; Fran Silver;
} Carol Bassett; Victoria Rennesund; Stephanie Shaw; Andrea Sands; Bridgette
} Berryhill; Shari Hamilton; Sharon Bevis; Jane Birkhold; Darbi Bossman;
} Jennifer Capouya; Cindy Daniels; Pat Fox; Bev Kinsey; Nancy Leeds; Wendy
} McCoy; Ilene Beer; Sarah Shanks; Alina Simonds; Erin Stuart; Katya Bock;
} Lowie Bock; Connie Zink
} Cc: Mary DeLong
} Subject: Fw: Easy way to help a good cause TOMORROW
}
}
} } Subject: Easy way to help a good cause TODAY - Oct .24
} }
} }
} }
} }
} }
} } Subject: FW: Breast Cancer & the NFL
} }
} }
} } The NFL has chosen October 24, 2000 as the NFL's Breast Cancer
} } } } } Awareness day. Every time someone logs onto www.NFL.com, the NFL
} } will
} } } } } donate $5 to the Susan G. Komen Breast Cancer Foundation. This
} is
} a
} } } } simple
} } } } } opportunity to learn about one of our fabulous National Series
} } Sponsor
} } } } of
} } } } } the Komen Race for the Cure(R), as well as earn up to $50,000 for
} the
} } } } Komen
} } } } } Foundation. Tell all your friends and co-workers on October 24
} to
} } log
} } } } on!!
} }
}



From daemon Tue Oct 24 13:20:56 2000



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Tue, 24 Oct 2000 13:15:29 -0500
Subject: D19b developer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Richard,

Photographic developers that are no longer available can often be made from
scratch using just a few standard ingredients that are fairly readily
available. It may be that Eastman Kodak would consent to give you the
formula if asked, but if not, chances are pretty good that the formula is
out there floating around somewhere in an old book, journal, or even in
cyberspace.

I know that most people don't want to mess around with making their own
developers, or don't feel secure using them, but if you're interested (and
not in a huge hurry) I can check some sources to try and locate the formula.
It is probably just a minor variant of regular Kodak D-19. I have a small
collection of darkroom chemistry oriented literature, plus some good leads
on where to ask.

Good luck,
Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/





-----Original Message-----
} From: Richard Beanland +44 1327 356363
[mailto:richard.beanland-at-marconi.com]
Sent: Tuesday, October 24, 2000 12:38 PM
To: Microscopy Listserver


Hi All,
I am looking for a good replacement for D19b developer (for nuclear
emulsion plates, as used in our X-ray radiography and topography images, no
longer available). We are using Suprol at the moment, but would like
something as good as the old stuff. Anyone have any ideas?

Richard

==============================================================
Richard Beanland,
Structural Analysis Lab,
Caswell Technology,
Caswell,
Towcester,
Northants NN12 8EQ

e-mail richard.beanland-at-marconi.com
Tel. +44 1327 356363
Fax. +44 1327 356398
==============================================================
"The information contained in this message is legally privileged and
confidential information intended for the eyes of the individual or entity
named above. If the reader of this message is not the intended recipient,
you are hereby notified that any dissemination, distribution or copying of
this message is strictly prohibited. If you have received this message in
error, please notify us immediately by telephone.
Caswell Technology is the trading name of Marconi Caswell Limited.
Registered in London No. 3694360 Registered Office: One Bruton Street London
W1X 8AQ. Holding Company: Marconi plc."







From daemon Tue Oct 24 14:45:22 2000



From: Fiona Graham :      graham-at-scifs1.und.ac.za
Date: Tue, 24 Oct 2000 21:38:49 +0200
Subject: Link ISIS v. 3 users

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Does anyone know of software that will do a batch conversion of the
.IM_ image files captured using Link ISIS v. 3 Autobeam, to a standard
format (eg. pcx, jpg, etc)?

Fiona
____________________
Dr Fiona Graham
EM Unit,
University of Natal, Durban
South Africa

**** Remember ICEM-15 in 2002, is in Durban, South Africa ****
www.icem15.com




From daemon Tue Oct 24 15:13:46 2000



From: Jo Dee Fish :      jofish-at-burnham-inst.org
Date: Tue, 24 Oct 2000 13:10:01 -0700
Subject: Re: Uranyl Nitrate

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ruth,
0.01% Uranyl Nitrate is used as a local catalyst in the polymerization of
methacrylates.

Hayat MA. Principles and Techniques of Electron Microscopy. 1970. Van Nostrand
Reinhold, NY.

Hope this helps!
Jo Dee

Sara Miller wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} On Mon, 23 Oct 2000, Ruth Yamawaki wrote:
}
} } Date: Mon, 23 Oct 2000 15:52:51 -0700
} } From: Ruth Yamawaki {Ryamawaki-at-cmexchange.stanford.edu}
} } To: "'Microscopy-at-sparc5.microscopy.com'"
} {Microscopy-at-sparc5.microscopy.com}
} } Subject: Uranyl Nitrate
} }
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Our health and safety officer wants to know if Uranyl Nitrate is used as an
} } EM stain. Does anyone use Uranyl Nitrate? He did not tell me why he needs
} } to know.
} }
} } Thanks,
} }
} } Ruth
} }
} } ***************************************
} } Ruth Yamawaki
} } Department of Comparative Medicine
} } Stanford University
} } Stanford, CA 94305
} } ***************************************
} }
} Uranyl nitrate, like uranyl acetate and other uranium salts, is used as a
} stain in EM. The salt most frequently used is uranyl acetate because of
} its staining efficiency. Uranium has a heavy atomic nucleus (92) and is
} the heaviest metal used as a stain in EM. In tissue, it stabilizes
} nucleic acids and membranes when used prior to embedding. It is
} frequently used after sections are cut to "post stain" or further
} increase the contrast of membranes, etc., in sections. Uranyl salts,
} particularly acetate, are also used as negative stains for small things
} such as viruses and small cellular organelles in suspension. The stain
} darkens the background support and cracks and crevasses in the sample to
} add contrast. Uranyl salt solutions should be handled with care because
} they are radioactive. The alpha radiation is generally contained by
} glass (lead not necessary), but nonetheless, one should not leave dried
} pipets and droplets lying around to become airborne and possibly inhaled.
}
} Hayat MA. Positive Staining for Electron Microscopy. 1975. Van Nostrand
} Reinhold, NY.
}
} Hayat MA, Miller SE. 1990. Negative Staining. McGraw-Hill, NY.
}
} Sara E. Miller, Ph. D.
} P. O. Box 3712
} Duke University Medical Center
} Durham, NC 27710
} Ph: 919 684-3452
} FAX: 919 684-3265

--
Jo Dee Fish
Coordinator of Electron Microscopy
Cell Analysis Facility
The Burnham Institute
10901 N. Torrey Pines Rd.
La Jolla, CA 92037
(858)646-3100 ext. 3620





From daemon Tue Oct 24 15:59:38 2000



From: Geoff McAuliffe :      mcauliff-at-UMDNJ.EDU
Date: Tue, 24 Oct 2000 16:54:19 -0400
Subject: Re: D19b developer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Richard Beanland +44 1327 356363 wrote:

} -Hi All,
} I am looking for a good replacement for D19b developer (for nuclear emulsion plates, as used in our X-ray radiography and topography images, no longer available). We are using Suprol at the moment, but would like something as good as the old stuff. Anyone have any ideas?
}
} Richard
}
} ==============================================================
} Richard Beanland,
} Structural Analysis Lab,
} Caswell Technology,
} Caswell,
} Towcester,
} Northants NN12 8EQ
}
} e-mail richard.beanland-at-marconi.com
} Tel. +44 1327 356363
} Fax. +44 1327 356398
}

How about mixing your own from scratch? I will see if I can dig up the formula tomorrow (Wed).

Geoff
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************





From daemon Tue Oct 24 16:02:06 2000



From: COURYHOUSE-at-aol.com
Date: Tue, 24 Oct 2000 17:07:40 EDT
Subject: Re: D19b developer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Just for your information: A camera Lucida is also called a Drawing Tube and
even sometimes Une Chambre claire.
It does not project the image observed thru the microscope but allowed to
overimposed the image seen thru the microscope and a piece of paper located
on the side of the microscope and at the same time the pencil on this paper.
The brightness if the paper as the be the same as the image observed to see
both field at the same time. Than one just have to follow the contour of the
object to obtain the right drawing.
It exist drawing tubes for Compond microscopes as well as stereo`s
microscopes
My two cents information
Norm

----- Original Message -----
} From: Frank Thomas {thomasf-at-agc.bio.ns.ca}
To: {Microscopy-at-sparc5.microscopy.com} ; {hma-at-tupphysiol1.bp.dal.ca}
Sent: Tuesday, October 24, 2000 07:28


Folks we have some pretty definitive formularies for photographic chemicals
here at the museum.
Contact us off list with a wish list of developer recipes if you have not
found anything.

thanks Ed Sharpe archivist for SMECC

{ { Subj: RE: D19b developer
Date: 10/24/00 1:43:45 PM US Mountain Standard Time
From: TindallR-at-missouri.edu (Tindall, Randy D.)
To: richard.beanland-at-marconi.com ('Richard Beanland +44 1327 356363')
CC: microscopy-at-sparc5.microscopy.com ('microscopy-at-sparc5.microscopy.com')

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America
To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
-----------------------------------------------------------------------.


Richard,

Photographic developers that are no longer available can often be made from
scratch using just a few standard ingredients that are fairly readily
available. It may be that Eastman Kodak would consent to give you the
formula if asked, but if not, chances are pretty good that the formula is
out there floating around somewhere in an old book, journal, or even in
cyberspace.

I know that most people don't want to mess around with making their own
developers, or don't feel secure using them, but if you're interested (and
not in a huge hurry) I can check some sources to try and locate the formula.
It is probably just a minor variant of regular Kodak D-19. I have a small
collection of darkroom chemistry oriented literature, plus some good leads
on where to ask.

Good luck,
Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/


} }



From daemon Tue Oct 24 16:56:09 2000



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Tue, 24 Oct 2000 15:49:55 -0600
Subject: RE: PhotoShop usage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Michael Shaffer wrote:

} The caveat here is the "conversion" when you do this ... that is, PS
} makes the simple opening of the file such an innocent process, the
} user may not notice his/her data has been modified. What was once a
} pixel value of '128' is now '183'!!

I don't want to put down Photoshop, especially since we are producing a
competing product, but I think this remark points to one problem that many
"photo" applications have -- they do not treat the intensity as scientific
data. I may be mistaken, but I think Photoshop is conceptually a
"beautifying" software to make photos "look nicer" -- and it does a great
job at this, but it may also be lacking in certain areas. I'd be interested
if Photoshop can deal with inverse length scales such as on diffraction
patterns, or if it can calibrate the intensity in values such as non linear
length scales or other values. In the case Michael mentions above, for
example, one should be able to change the look-up table to optimize the
display without changing the underlying data.

Don't misunderstand me, please. I think Photoshop is a great product for
what it does, but for heavy duty data reduction and quantitative analysis
another software may be a better choice (Sorry if this sounds like a "plug"
for ours, there are many others available as well).

Michael

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: michael shaffer [mailto:epmalab-at-darkwing.uoregon.edu]
Sent: Tuesday, October 24, 2000 8:32 AM
To: Microscopy-at-sparc5.microscopy.com


Bill Miller writes ...

} ... I said PhotoShop, and was asked how he would use it.
} I told him the standard things - contrast
} enhancement, color matching across different media,
} annotation, resizing, etc. ...
} ...
} how else is PhotoShop being used - anything fancy to
} justify the cost?

We use PS5 here for annotation and presentation of spacial elemental
mapping (elemental x-ray maps), and average atomic number maps
(backscatter electron images). There isn't better software for
presenting this type of data in a "perceptually" uniform way. For
example, if you have a grayscale elemental map which represents SiO2
from 0% to 100%, with most softwares and typical monitors the change
from 20% to 30% isn't perceived the same as 70% to 80%, if perceived
at all. Opening and converting such an image into a Photoshop
gamma=2.2 working space makes this gradation perceptually uniform, and
much better for presentation.
The caveat here is the "conversion" when you do this ... that is, PS
makes the simple opening of the file such an innocent process, the
user may not notice his/her data has been modified. What was once a
pixel value of '128' is now '183'!! The data is now useless for
quantitative analysis. Make it a practice to keep (possibly even
write-protect) your original image files, and put your PS projects in
a separate directory.

cheerios, =shAf= :o)

{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {}
Michael Shaffer, R.A. - mshaf-at-darkwing.uoregon.edu
Geological Science's Electron Probe Facility - University of Oregon
http://epmalab.uoregon.edu/






From daemon Tue Oct 24 17:40:47 2000



From: jmkrupp-at-cats.ucsc.edu (Jon Krupp)
Date: Tue, 24 Oct 2000 15:37:29 -0700
Subject: D-19 developer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At last a chance to use some ancient knowledge to help out.

For Kodak products, there was a convention that said anything with a number
as a name was a public domain type formula. So if you go into some old
Kodak books you will find the formulas for things like D-19, D-11, D-76
etc.

Products that used a name for a name, like Dektol, were proprietary
formulas, not generally available.

I probably have the formulas for most of the standard type numbered
products. Let me know if you need one.

Jonathan Krupp
Microscopy & Imaging Lab
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu





From daemon Tue Oct 24 19:01:15 2000



From: Jeff Stewart :      jeff-at-metallography.com
Date: Tuesday, October 24, 2000 12:19 PM
Subject: Re: Setting the record straight ...

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Knock off the political mumbo-jumbo. It is surely inappropriate here.

Nestor can you filter this stuff?
-----Original Message-----
} From: William R. Oliver {oliver-at-cpt.afip.org}
To: Massimo Catalano {massimo.catalano-at-ime.le.cnr.it}
Cc: microscopy-at-sparc5.microscopy.com {microscopy-at-sparc5.microscopy.com}




From daemon Tue Oct 24 19:47:13 2000



From: Kun Li :      k-li-at-mailcityasia.com
Date: Wed, 25 Oct 2000 08:39:03 +0800
Subject: Re: Enlarger

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Jinguo,

I think De Vere 504 is a good one. Depending on your need and the type of paper you use, you can choose different enlarger heads such as Ilford Ilfospeed Multigrde enlarger head.

Regards,

Kun Li

Systems on Silicon Manufacturing Company Pte Ltd
FA Section
--

On Tue, 24 Oct 2000 09:03:27 Jinguo Wang wrote:
} ------------------------------------------------------------------------
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From daemon Wed Oct 25 01:55:03 2000



From: Gordon Couger :      gcouger-at-couger.com
Date: Wed, 25 Oct 2000 01:51:02 -0500
Subject: Re: D19b developer

Contents Retrieved from Microscopy Listserver Archives
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I have read that some dilution of Kodak HC110 made a good substitute for D
19. A call to Kodak's help line 800 242 2424 should get an answer. HC110
keeps for ever and can be replenished.

Gordon
Gordon Couger gcouger-at-couger.com
Stillwater, OK www.couger.com/gcouger

} From: "Geoff McAuliffe" {mcauliff-at-UMDNJ.EDU}
} } -Hi All,
} } I am looking for a good replacement for D19b developer (for
nuclear emulsion plates, as used in our X-ray radiography and topography
images, no longer available). We are using Suprol at the moment, but
would like something as good as the old stuff. Anyone have any ideas?
} }
} } Richard
} }
} } ==============================================================
} } Richard Beanland,
} } Structural Analysis Lab,
} } Caswell Technology,
} } Caswell,
} } Towcester,
} } Northants NN12 8EQ
} }
} } e-mail richard.beanland-at-marconi.com
} } Tel. +44 1327 356363
} } Fax. +44 1327 356398
} }
}
} How about mixing your own from scratch? I will see if I can dig up the
formula tomorrow (Wed).
}
} Geoff
} --
} **********************************************
} Geoff McAuliffe, Ph.D.
} Neuroscience and Cell Biology
} Robert Wood Johnson Medical School
} 675 Hoes Lane, Piscataway, NJ 08854
} voice: (732)-235-4583; fax: -4029
} mcauliff-at-umdnj.edu
} **********************************************
}
}
}





From daemon Wed Oct 25 06:08:42 2000



From: Richard Gardiner :      rbgardiner-at-home.com
Date: Wed, 25 Oct 2000 07:05:35 -0400
Subject: Developer D19b

Contents Retrieved from Microscopy Listserver Archives
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Here is a formulae for Developer D19b I have in an old file.


Metol 2.2
Hydroquinone 8.8
Sodium sulphite(anhydrous) 72
Sodium carbonate(anhydrous) 48
Potassium bromide 4
Water to 1 L.


D-19b was generally regarded as a high contrast developer for X-ray and
industrial photography. Used undiluted at 68F, the average time for
tank development is five minutes. This is a well balanced developer of
good keeping properties.

Richard Gardiner
Agriculture Canada




From daemon Wed Oct 25 06:43:00 2000



From: John Heckman :      heckman-at-pilot.msu.edu
Date: Wed, 25 Oct 2000 04:46:23 -0700
Subject: Re: D-19 developer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



D19b formula (quoted as a "manufacturer's formula" in British
Journal Photographic almanac for 1955)

metol 2.2g
hydroquinone 8.8g
sodium sulphite cryst. 144g
sodium carbonate cryst. 130g
potassium bromide 4g
water to 1litre

Chris

Date sent: Tue, 24 Oct 2000 17:37:32 +0000 (GMT)
} From: Richard Beanland +44 1327 356363 {richard.beanland-at-marconi.com}


If it's ordinary D-19 developer there are numerous sources for this formula
published. There's also one for a replenisher called D-19R (replenisher).
However, I've not seen a D-19b. I've used D-19 for TEM plates (Kodak SO-163
and 4489) for years and it's readily available in those familiar yellow
packages, at least here in the states. The formula (from Kodak's publication
J-1):

Water, about 50 degrees C 500ml

Kodak ELON Developing agent (aka Metol or p-methylaminophenol sulfate) 1.0
gram
Kodak Sodium sulfite
(anhydrous)
75.0 grams
Kodak
Hydroquinone
9.0 grams
Kodak Sodium Carbonate
(monohydrate) 30.0
grams
Kodak Potassium Bromide
(anhydrous)
5.0 grams Cold water to make 1 liter.

At a state-side price, for working solution, of about $5.00 for 3 gallons I
buy those little yellow packages. In formulating related photographic
solutions, I've found no difference in using equivalent bulk chemicals of
different manufacture. As I recall the above formula is also in the Ilford
manual (probably sans the Kodak trade names).

See what develops,

John

No financial interest in the Eastman-Kodak Company

Jon Krupp wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} At last a chance to use some ancient knowledge to help out.
}
} For Kodak products, there was a convention that said anything with a number
} as a name was a public domain type formula. So if you go into some old
} Kodak books you will find the formulas for things like D-19, D-11, D-76
} etc.
}
} Products that used a name for a name, like Dektol, were proprietary
} formulas, not generally available.
}
} I probably have the formulas for most of the standard type numbered
} products. Let me know if you need one.
}
} Jonathan Krupp
} Microscopy & Imaging Lab
} University of California
} Santa Cruz, CA 95064
} (831) 459-2477
} jmkrupp-at-cats.ucsc.edu




From daemon Wed Oct 25 06:56:29 2000



From: John Heckman :      heckman-at-pilot.msu.edu
Date: Wed, 25 Oct 2000 04:57:56 -0700
Subject: Woops, that was D-11

Contents Retrieved from Microscopy Listserver Archives
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Sorry Listers,

My posting for the D-19 formula was wrong. My message was for D-11 which
I've used for higher contrast work. Looks like the correct D-19b
formula has been posted. It's different from regular D-19.

sorry folks,

John



From daemon Wed Oct 25 07:11:37 2000



From: =?iso-8859-2?B?TGVzemVrIEvqcGnxc2tp?= :      kepinski-at-int.pan.wroc.pl
Date: Wed, 25 Oct 2000 14:13:01 +0200
Subject: TEM-sensitive samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


D.M. de Leeuw et al. Physica C 158 (1989) 391 prepared air and moisture
sensitive samples for TEM by crushing them in a mortar under decanol and
putting a droplet of the suspension onto a grid. Decanol has low evaporation
rate and thus protects the sample. However, it evaporates inside microscope
in air-lock but probably also in the column. I would like to know if the
procedure is save for modern UHV microscopes and/or there are other
procedures that could be safely used for similar air sensitive samples.

Leszek Kepinski

Institute of Low Temperature and Structure Research,
Polish Academy of Sciences,
P.O.Box 1410,
50-950 Wroclaw, Poland

e.mail : kepinski-at-int.pan.wroc.pl,
http://www.int.pan.wroc.pl





From daemon Wed Oct 25 07:58:50 2000



From: William R. Oliver :      oliver-at-cpt.afip.org
Date: Wed, 25 Oct 2000 07:20:57 -0400 (EDT)
Subject: Re: Setting the record straight ...

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On Tue, 24 Oct 2000, Jeff Stewart wrote:

} Knock off the political mumbo-jumbo. It is surely inappropriate here.
}
} Nestor can you filter this stuff?


Sheesh. Gals and guys, it was a *joke,* see. You know, there was this
big flap about a certain politician inserting subliminal messages into
things (even though he couldn't *pronounce* the word), and I was trying
to leaven my reply to Dr. Catalano with a little humor. You know --
inserting inappropriate short statements sotto voce. Ever see Kevin
Nealon's schtick on "Subliminal Man"?


As described by Dr. Gary Radford in "Subliminal Persuasion in the
Mass Media" http://alpha.fdu.edu/~gradford/subliminal.html:

Kevin Nealon, a comic on Saturday Night Live, portrays a character
called "Subliminal Man." Subliminal Man persuades people to do his
bidding by uttering "hidden," but in fact wholly audible messages under
his breath. In a commercial for Miller Lite, he says: "What they won't
do in commercials these days [buy Miller Lite] - I mean, they resort to
all sorts of tactics [brainwash]. Now take Miller Lite [six pack], it
has a great pilsner taste [gonna love it]." At the end of the ad,
Subliminal Man propositions a beautiful blonde: "Hi [your place], would
you like to join me for a Lite [your treat]? It's on me." She coos
back: "OK, but it's my treat."


And, you see, it *had* to be Bush, because he was the one in the news
about it.

Sorry, I had made the obviously silly assumption that this was
a pretty transparent schtick which merely attempted to sugar-coat the
real message, which was that frank poltical discussions *were*
inappropriate. You chose to ignore *that* message and instead only
read the bullet insertions between the paragraphs.

I would make some correllations between stiffness, lack of sense
of humor and likely candidate support, but I will leave that be
for now.

Henceforth I promise never to use humor in this group and *only*
reply in terms of self-righteous outrage and stern condemnation.


billo




From daemon Wed Oct 25 08:35:39 2000



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Wed, 25 Oct 2000 08:26:45 -0500
Subject: Equipment insurance contracts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear listers,

I hope this isn't an inappropriate question, but we have some quick
decisions to make.

I've queried the list a couple times before about peoples' experiences with
non-OEM equipment insurance contracts, but now I need to ask a more specific
question. I would be very interested in hearing from people who have
experience with CIC Corp and/or Kemper Cost Management, particularly with
electron microscopes and ancillary equipment. Both positive and negative
comments are very welcome.

Please (pretty please?) reply to me directly and not to the listserver,
especially if you have negative comments. I think Nestor frowns on public
company bashing and I don't blame him.

Thanks much.

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/






From daemon Wed Oct 25 09:28:15 2000



From: Anjeanette Ormonde :      ormonde-at-chem.nwu.edu
Date: Wed, 25 Oct 2000 09:27:28 -0500 (CDT)
Subject: Suggestions needed/stereoscopy?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,
I am a graduate student and I need some help trying to figure out
a quantitative way to evaluate my samples before running my experiments
with them. I am using a technique we call nanosphere lithography by which
we deposit polystyrene spheres onto a substrate. We want a single layer
of hexagonally packed spheres. My spheres are 400nm across which means
the spaces between them are just under 100nm. We get this packing in some
areas and can see a nice, dark blue color. However, just as in crystal
growth we also see grain boundries and other defects where we may have no
spheres or multiple layers of spheres. My substrates are 12 - 15 mm
square ITO coated glass. AFM is not a good solution because it takes
entirely too long to look at an entire sample and even then, I only have
my observations to say which area is the best. Someone mentioned
stereology to me but I am unsure of what that technique entails and what
information I can gain from it. I am looking for something that will be
able to look at the entire sample and eventually give me some sort of
number such as the % that is perfect or even the % that is defective. We
have an 80X objective but that isn't enough resolution to see the tiny
spheres in enough detail. However, I have access to some other facilities
on campus but need to know what to look for. Does anyone have any
suggestions? Thanks for your time and any comments are appreciated.

Angie Ormonde





From daemon Wed Oct 25 09:49:31 2000



From: Timothy Schneider :      Timothy.Schneider-at-Mail.TJU.EDU
Date: Wed, 25 Oct 2000 10:35:31 -0400
Subject: RE: ENLARGER

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have been very happy with my Durst Point Source enlarger with a
varicontrol illuminator. However before you invest over 10K in such a
device, you really should investigate the possibilities of a CCD camera
upgrade for your scope. I was at the Microscopy and Microanalysis 2000
meeting this past summer in Philadelphia, and based on the many different
vendors of such devices, it is obvious that the days of film are not
unlimited. While a CCD camera and its related computer needs are expensive
up front, the product they produce is very manuscript and grant application
form friendly. For labs with space restrictions, a computer work station
certainly takes up less square footage of floor space compared to a dark
room and does not have to expose the operator and the environment to
potentially hazardous chemistry that is associated with conventional dark
room operations. The down side of CCD imaging is the upfront cost and not
quite as high resolution compared to film. Tim

Timothy G. Schneider
Director of Electron Microscopy
Department of Pathology
Room 229 Jefferson Hall
Thomas Jefferson University
1020 Locust St.
Philadelphia Pa. 19107
215-503-4798 work
610-613-8170 cellular
timothy.schneider-at-mail.tju.edu




From daemon Wed Oct 25 09:53:22 2000



From: Chris Jeffree :      cjeffree-at-srv0.bio.ed.ac.uk
Date: Wed, 25 Oct 2000 15:51:05 +0000
Subject: Re: Setting the record straight ...

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Speaking of which I have a self-righteous stage from a condemned stem
free to a good home. Unfortunately it's contaminated with subliminable
materials, but could be cleaned with a solution of vacuous politicians in
HF. Replies by bush telegraph only please
Chris
}
} Henceforth I promise never to use humor in this group and *only*
} reply in terms of self-righteous outrage and stern condemnation.
}
}
} billo
}
}


==========================================
Dr. Chris Jeffree
University of Edinburgh
BIOSEM - Biological Sciences Electron Microscope Facility
Institute of Cell and Molecular Biology
Daniel Rutherford Building
King's Buildings, Mayfield Road
EDINBURGH, EH9 3JH, Scotland, UK
Tel. #44 (0) 131 650 5345
FAX. #44 (0) 131 650 6563
Mobile 07710 585 401
email c.jeffree-at-ed.ac.uk
=========================================



From daemon Wed Oct 25 09:59:38 2000



From: michael shaffer :      epmalab-at-darkwing.uoregon.edu
Date: Wed, 25 Oct 2000 07:43:03 -0700
Subject: microscope + coolpix camera problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We are presently purchasing several student polarizing
microscopes, plus one which can be used with a digital camera for
classroom presentation. We are initially considering the Coolpix
950/990 with its appropriate adapter on a 1X C-mount.
During a demo yesterday, we were quite impressed with a Coolpix's
NTSC output ... color as good as we've seen with more expensive
cameras. However, we were a bit disappointed when we tried to grab a
digital image. The camera would vignette much more than the video
display would indicate (there was actually no vignetting with the NTSC
out) ... although the color is quite accurate. But before I point you
at examples, let me first explain we tried this demo with my personal
CP800, believing we should get performance very similar to the CP950.
The vignetting problem may be due to a feature setting available on
the 950 I am not aware of.
Point your browser at the URLs below ... the 1st is full image, so
you can inspect the detail ... the 2nd will show you the vignetting.

http://darkwing.uoregon.edu/~mshaf/dogsci/x-biot.jpg
http://darkwing.uoregon.edu/~mshaf/dogsci/x-biot-sm.jpg

cheerios, shAf :o)

{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {}
Michael Shaffer, R.A. - mshaf-at-darkwing.uoregon.edu
Geological Science's Electron Probe Facility - University of Oregon
http://epmalab.uoregon.edu/





From daemon Wed Oct 25 10:11:11 2000



From: Walck, Scott D. :      walck-at-ppg.com
Date: Wed, 25 Oct 2000 11:09:08 -0400
Subject: RE: Suggestions needed/stereoscopy?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


As a starter, you need to get your hands on John Russ' Image Analysis
Handbook to find out what stereology or Quantitative Microscopy can do for
you. There are other books that you can find on the subject, but they are
cited in his book.

I would suspect that you might be able to process your using an FFT to
filter the periodic structure so that the grain boundaries are enhanced and
then further enhance the grain boundaries so that you can quantify them.
There are a couple of standard quantitative measurements that you can make
at this point to characterize your microstructure. You can do the
measurements by hand, but the computer is much easier. John Russ has a
series of Plug-ins for doing these measurements and image enhancements in
Photoshop and NIH image that are a companion to his book.

There are also a number of other commercial products out there that might be
able to help you with this, but they would be more expensive. NIH Image is
an alternative software package that is free and John's plug-ins also work
there.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)



} -----Original Message-----
} From: Anjeanette Ormonde [mailto:ormonde-at-chem.nwu.edu]
} Sent: Wednesday, October 25, 2000 10:27 AM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Suggestions needed/stereoscopy?
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.ht} ml
}
}
} --------------------------------------------------------------
} ---------.
}
}
} Hi,
} I am a graduate student and I need some help trying to
} figure out
} a quantitative way to evaluate my samples before running my
} experiments
} with them. I am using a technique we call nanosphere
} lithography by which
} we deposit polystyrene spheres onto a substrate. We want a
} single layer
} of hexagonally packed spheres. My spheres are 400nm across
} which means
} the spaces between them are just under 100nm. We get this
} packing in some
} areas and can see a nice, dark blue color. However, just as
} in crystal
} growth we also see grain boundries and other defects where we
} may have no
} spheres or multiple layers of spheres. My substrates are 12 - 15 mm
} square ITO coated glass. AFM is not a good solution because it takes
} entirely too long to look at an entire sample and even then,
} I only have
} my observations to say which area is the best. Someone mentioned
} stereology to me but I am unsure of what that technique
} entails and what
} information I can gain from it. I am looking for something
} that will be
} able to look at the entire sample and eventually give me some sort of
} number such as the % that is perfect or even the % that is
} defective. We
} have an 80X objective but that isn't enough resolution to see the tiny
} spheres in enough detail. However, I have access to some
} other facilities
} on campus but need to know what to look for. Does anyone have any
} suggestions? Thanks for your time and any comments are appreciated.
}
} Angie Ormonde
}
}
}



From daemon Wed Oct 25 11:30:12 2000



From: Frank Thomas :      thomasf-at-AGC.BIO.NS.CA
Date: Wed, 25 Oct 2000 13:26:10 -0300
Subject: Cleaning a Faraday Cage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Listers -

Does anybody know a good way to clean a dirty Faraday cage? (I know, I
shouldn't have let it get dirty in the first place....:-() I'm thinking
sonification in soapy water with lots of rinsing afterwards, but I wonder
if there are any drawbacks to this. I'm hesitant to use solvents because I
don't know what "glue" was used to attach the little aperture on top, and
it was way too expensive to screw up that way. I tried a puff of air from
one of those little rubber squeeze-bulb thingies (whatever the hell they're
called) but that just didn't do the trick.
I don't really know what the contamination is but it's partially
transparent in a 20KeV beam. Could be anything but metal, I guess.....

F.C. Thomas
MicroAnalysis Facility
Bedford Institute of Oceanography
Dartmouth, Nova Scotia
Canada
Geological Survey of Canada (Atlantic)
Bedford Institute of Oceanography
Dartmouth, Nova Scotia
Canada B2Y 4A2



From daemon Wed Oct 25 12:02:42 2000



From: Larry D. Ackerman :      mishot-at-itsa.ucsf.edu
Date: Wed, 25 Oct 2000 09:57:50 -0700
Subject: Enlarger

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The enlargers made by Durst, Beseler and Omega have always been considered
among the best. A condenser system for the light source and a point light
source will give you the sharpest image. I have had several Durst enlargers
because of their nice point light source. It helps to buy the best lens
available. Schneider lenses have a good reputation (Apo Rodagon etc). The
enlarger usually must be one designed for use with up to 4" X 5" negatives
in order to acommodate the EM negatives. In the past I purchased special
negative carrierers from a small company on the east coast named Carlwen. I
recommend getting assistance from your local professional photography dealer.
Larry D. Ackerman
Lily & Yuh Nung Jan Laboratories
Howard Hughes Medical Institute
UCSF, Box 0725, Rm U226
533 Parnassus Ave.
San Francisco, CA 94143

(415) 476-8751 FAX (415) 476-5774
mishot-at-itsa.ucsf.edu



From daemon Wed Oct 25 12:10:19 2000



From: Richard Beanland +44 1327 356363 :      richard.beanland-at-marconi.com
Date: Wed, 25 Oct 2000 18:08:18 +0000 (GMT)
Subject: D19b developer - thanks

Contents Retrieved from Microscopy Listserver Archives
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A huge thankyou to the many of you who responded to my query about D19b. Several people gave me the recipe, and one kind person even had a can of the stuff they were willing to send to me! I am a happy man.

Thanks again,

Richard

==============================================================
Richard Beanland,
Structural Analysis Lab,
Caswell Technology,
Caswell,
Towcester,
Northants NN12 8EQ

e-mail richard.beanland-at-marconi.com
Tel. +44 1327 356363
Fax. +44 1327 356398
==============================================================
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Caswell Technology is the trading name of Marconi Caswell Limited. Registered in London No. 3694360 Registered Office: One Bruton Street London W1X 8AQ. Holding Company: Marconi plc."







From daemon Wed Oct 25 12:18:22 2000



From: Tyrone L. Daulton :      tdaulton-at-nrlssc.navy.mil
Date: Wed, 25 Oct 2000 12:15:18 -0500
Subject: air sensitive specimens

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Dr. Kepinski

I assume that you mean oxygen sensitive samples when you say air
sensitive.

In that event you might try crushing the specimen in a glove box/bag
purged with a continual flow of dry nitrogen gas.
You can mount the specimen on the TEM grid and then the grid in the TEM
holder all within the glove bag. Exposure to
oxygen and atmospheric water vapor would be limited to the brief
interval between removing the holder from the
bag and inserting it into the microscope column. In fact, I have seen
people do all this in a nitrogen purged plastic bag that also
encloses the TEM's airlock to avoid oxygen exposure during transfer to
the airlock.

This may be worth a try if you are concerned about Decanol evaporation
in the TEM column.



} D.M. de Leeuw et al. Physica C 158 (1989) 391 prepared air and moisture

} sensitive samples for TEM by crushing them in a mortar under decanol
and
} putting a droplet of the suspension onto a grid. Decanol has low
evaporation
} rate and thus protects the sample. However, it evaporates inside
microscope
} in air-lock but probably also in the column. I would like to know if
the
} procedure is save for modern UHV microscopes and/or there are other
} procedures that could be safely used for similar air sensitive samples.

}
} Leszek Kepinski

--
_____________________________________________________
Tyrone L. Daulton
Director: Marine Geosciences - Electron Microscopy Center
Physicist and Senior Electron Microscopist

Naval Research Laboratory
Marine Geosciences Division (Code 7400)
Stennis Space Center, MS 39529-5004

Voice (228)-688-4877
Fax (228)-688-4093
email tdaulton-at-nrlssc.navy.mil
tld-at-howdy.wustl.edu





From daemon Wed Oct 25 12:27:17 2000



From: auto_quoter :      auto_quoter-at-yahoo.com
Date: Wed, 25 Oct 2000 10:23:59 -0700
Subject: FREE! Get A Great Price On A New Car! (61590264)

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From daemon Wed Oct 25 12:27:56 2000



From: William F. Tivol :      wft03-at-health.state.ny.us
Date: Wed, 25 Oct 2000 13:25:32 -0400
Subject: Re: Suggestions needed/stereoscopy?

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I am a graduate student and I need some help trying to figure out
a quantitative way to evaluate my samples before running my experiments
with them. I am using a technique we call nanosphere lithography by which
we deposit polystyrene spheres onto a substrate. We want a single layer
of hexagonally packed spheres. My spheres are 400nm across which means
the spaces between them are just under 100nm. We get this packing in some
areas and can see a nice, dark blue color. However, just as in crystal
growth we also see grain boundries and other defects where we may have no
spheres or multiple layers of spheres. My substrates are 12 - 15 mm
square ITO coated glass. AFM is not a good solution because it takes
entirely too long to look at an entire sample and even then, I only have
my observations to say which area is the best. Someone mentioned
stereology to me but I am unsure of what that technique entails and what
information I can gain from it. I am looking for something that will be
able to look at the entire sample and eventually give me some sort of
number such as the % that is perfect or even the % that is defective. We
have an 80X objective but that isn't enough resolution to see the tiny
spheres in enough detail. However, I have access to some other facilities
on campus but need to know what to look for. Does anyone have any
suggestions? Thanks for your time and any comments are appreciated.

Dear Angie,
Here are a couple of suggestions. I don't know how practical they are, but
they may suggest more practical possibilities. To get the average number of
spheres per unit area, you could try to measure the fraction of incident light
absorbed. Illuminate the specimen with a wavelength of light which is at the
absorption max, and either collect all the light which is not absorbed
(including scattered light), or measure the energy absorbed with a calorimeter.
The latter does not require collecting the scattered light, but may have other
problems, such as accounting for heat re-radiated. You would need to determine
the absorption of one sphere by calculating it or by measurement of a dilute
suspension of spheres in a medium which does not absorb light at the wavelength
selected and which has the same index of refraction as the spheres.
To get a measurement of the packing, you could try to measure the
diffraction of a parallel beam of light incident on the specimen. This time,
the spheres should not absorb at the wavelength selected. You could calculate
the expected intensity and compare this with what is observed. The spread of
the diffraction spots will give a measurement of the regularity of the crystal,
and the appearance of two or more distinct hexagonal patterns will tell you that
there is more than one patch being illuminated, and that the patches are about
the same size as the illuminating spot--assuming that the spot is not on the
boundary between two large patches (which is easily determined). The appearance
of rings or circles of many spots will indicate that many small patches are
being illuminated, and other possible patterns can be interpreted.
As I said, there are no guarrantees that either of these measurements are
useful, but good luck.
Yours,

Bill Tivol
Wadsworth Center
Albany NY
(518) 473-7399 WFT02-at-health.state.ny.us





From daemon Wed Oct 25 13:36:19 2000



From: Thomas Litzinger :      thomas_c_litzinger-at-yahoo.com
Date: Wed, 25 Oct 2000 11:31:40 -0700 (PDT)
Subject: suggestions for SEM newt/squid eye fixation protocol

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Hello everyone,
I was wondering if anyone had suggestions on a
protocol for the fixation of either newt or squid eye
to be viewed in the SEM.
I will be viewing a crossection of the eye. Would
it be best to fix after cutting the eye, or try to
perform a frozen fracture after fixation and
dehydration.
Any suggestions will be appreciated.
Thanks,
Thomas


__________________________________________________
Do You Yahoo!?
Yahoo! Messenger - Talk while you surf! It's FREE.
http://im.yahoo.com/



From daemon Wed Oct 25 15:54:01 2000



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Thu, 26 Oct 2000 09:53:44 GMT+1200
Subject: Re: Cleaning a Faraday Cage

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Sorry I don't know the answer to your quaestion, but I just can't
stop myself from asking:-

- How do you know it's dirty?
- How would you have prevented it from getting dirty?
- Does it matter, anyway?

cheers

rtch


}
} Does anybody know a good way to clean a dirty Faraday cage? (I
} know, I
} shouldn't have let it get dirty in the first place....:-() I'm
} thinking sonification in soapy water with lots of rinsing
} afterwards, but I wonder if there are any drawbacks to this. I'm
} hesitant to use solvents because I don't know what "glue" was used
} to attach the little aperture on top, and it was way too expensive
} to screw up that way. I tried a puff of air from one of those little
} rubber squeeze-bulb thingies (whatever the hell they're called) but
} that just didn't do the trick.
} I don't really know what the contamination is but it's partially
} transparent in a 20KeV beam. Could be anything but metal, I
} guess.....


Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand



From daemon Wed Oct 25 16:21:34 2000



From: Gary Gaugler :      gary-at-gaugler.com
Date: Wed, 25 Oct 2000 14:13:06 -0700
Subject: Fwd: D-19

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} To: John Heckman {heckman-at-pilot.msu.edu}
} From: Gary Gaugler {gary-at-gaugler.com}
} Subject: D-19
}
} Kodak still makes D-19. Part numbers are:
}
} 1464593 1 gallon
} 1946045 5 gallons
}
} gary g.




From daemon Wed Oct 25 16:34:14 2000



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Wed, 25 Oct 2000 15:27:13 -0600
Subject: Suggestions needed/stereoscopy?

Contents Retrieved from Microscopy Listserver Archives
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Have you tried SEM? The size should be easily resolvable in any SEM. You may
have to coat your sample to avoid charging.

Then take your images and run some FFTs on them. That should give you
information about packing and also faults in the packing. For a quantitative
analysis you probably need to design some filters in Fourierspace, and do a
back transform, then analyze those images.

There are a bunch of books out there that explain things in detail. John
Russ' book comes to mind.

Good luck.

Michael

Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Anjeanette Ormonde [mailto:ormonde-at-chem.nwu.edu]
Sent: Wednesday, October 25, 2000 8:27 AM
To: Microscopy-at-sparc5.microscopy.com


Hi,
I am a graduate student and I need some help trying to figure out
a quantitative way to evaluate my samples before running my experiments
with them. I am using a technique we call nanosphere lithography by which
we deposit polystyrene spheres onto a substrate. We want a single layer
of hexagonally packed spheres. My spheres are 400nm across which means
the spaces between them are just under 100nm. We get this packing in some
areas and can see a nice, dark blue color. However, just as in crystal
growth we also see grain boundries and other defects where we may have no
spheres or multiple layers of spheres. My substrates are 12 - 15 mm
square ITO coated glass. AFM is not a good solution because it takes
entirely too long to look at an entire sample and even then, I only have
my observations to say which area is the best. Someone mentioned
stereology to me but I am unsure of what that technique entails and what
information I can gain from it. I am looking for something that will be
able to look at the entire sample and eventually give me some sort of
number such as the % that is perfect or even the % that is defective. We
have an 80X objective but that isn't enough resolution to see the tiny
spheres in enough detail. However, I have access to some other facilities
on campus but need to know what to look for. Does anyone have any
suggestions? Thanks for your time and any comments are appreciated.

Angie Ormonde





From daemon Wed Oct 25 16:44:04 2000



From: Gary Gaugler :      gary-at-gaugler.com
Date: Wed, 25 Oct 2000 14:41:53 -0700
Subject: Any Pixera 600CL or 600ES experiences

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Has anyone tried using these digicams on LMs?
They have high resolution and seem like reasonably priced.

Any problems?

tnx,
gary g.




From daemon Wed Oct 25 17:21:40 2000



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Wed, 25 Oct 2000 17:16:54 -0500
Subject: Re: microscope + coolpix camera problem

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I am not familiar with the CoolPix cameras and the NTSC out function. Do
they have that available right off the camera? Is your video monitor
overscanning? Many do so that you do not see the entire height or width of
the image. If so, the vignetting would not appear too bad until you grabbed
the image and saw it all. Some monitors include an underscan switch so you
can see the whole image.

I suppose someone (Barbara Foster maybe) is going to weigh in on the
subject of vignetting in general. It seems that there have been several
discussions on this a few months back. I thought the basic problem was
using a transfer lens of too low a power for the camera. I have the
opposite problem with our Pixera (or any other 1/3" camera). We are using a
0.5x transfer lens. A 0.33x would be best to match the field of view and
the picture, but they were quite a bit more than the 0.5x lenses.

Warren S.

At 07:43 AM 10/25/2000 -0700, you wrote:

} We are presently purchasing several student polarizing
} microscopes, plus one which can be used with a digital camera for
} classroom presentation. We are initially considering the Coolpix
} 950/990 with its appropriate adapter on a 1X C-mount.
} During a demo yesterday, we were quite impressed with a Coolpix's
} NTSC output ... color as good as we've seen with more expensive
} cameras. However, we were a bit disappointed when we tried to grab a
} digital image. The camera would vignette much more than the video
} display would indicate (there was actually no vignetting with the NTSC
} out) ... although the color is quite accurate. But before I point you
} at examples, let me first explain we tried this demo with my personal
} CP800, believing we should get performance very similar to the CP950.
} The vignetting problem may be due to a feature setting available on
} the 950 I am not aware of.
} Point your browser at the URLs below ... the 1st is full image, so
} you can inspect the detail ... the 2nd will show you the vignetting.
}
} http://darkwing.uoregon.edu/~mshaf/dogsci/x-biot.jpg
} http://darkwing.uoregon.edu/~mshaf/dogsci/x-biot-sm.jpg
}
} cheerios, shAf :o)
}
} {} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {}
} Michael Shaffer, R.A. - mshaf-at-darkwing.uoregon.edu
} Geological Science's Electron Probe Facility - University of Oregon
} http://epmalab.uoregon.edu/




From daemon Wed Oct 25 17:32:12 2000



From: John J. Bozzola :      bozzola-at-siu.edu
Date: Wed, 25 Oct 2000 17:28:46 -0500
Subject: Fuji S1 digital cams on LMs

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We just received our Fuji FinePix Pro S1 camera. Beautiful instrument!!

I have not had luck interfacing it the a light microscope since the
camera stubornly insists on having an autoNikkor lens attached to it.
The usual microscope adapters cause the camera to be uncommunicative
with the computer.

I KNOW that it can be interfaced with a LM and computer because I saw
it in action at M&M2000.

So, does anyone know how to do this?

Several people are holding their breaths on this one.....


John B.
--
####################################################################
John J. Bozzola, Ph.D.
Professor & Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
####################################################################



From daemon Wed Oct 25 18:58:58 2000



From: Richard Gardiner :      rbgardiner-at-home.com
Date: Wed, 25 Oct 2000 19:53:01 -0400
Subject: Re D-11

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John:

If its D-11 you are looking for here it is:

Water 500 mL
Elon 1.0 gm
Sodium Sulfite 75 gm
Hydroquinone 9.0 gm
Sodium carbonate 30 gm
Potassium Bromide 5 gm
Water to 1 L.

D-11 is a high contrast developer for films and plates, also for line
diagrams.

Dissolve chemicals in the order given. When less contrast is desired
the developer should be diluted with an equal volume of water.

Richard Gardiner
Agriculture Canada




From daemon Wed Oct 25 20:02:29 2000



From: John J. Bozzola :      bozzola-at-siu.edu
Date: Wed, 25 Oct 2000 19:57:59 -0500
Subject: Re: microscope + coolpix camera problem

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Warren,

I just had a demo yesterday of the 990 on a Leica Orthoplan II. The
vignetting you showed was not present on our demo. Perhaps the zoom
was set wrong or the wrong type of relay lens was used. I did see a
slight amt of vignetting but it was minor--nothing like what you
showed.

We are definitely going to buy the 990 along with relay lens, wired
remote control and AC adapter. We got a price of $1864.


I really liked the software too.

JB

--
####################################################################
John J. Bozzola, Ph.D.
Professor & Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
####################################################################



From daemon Wed Oct 25 22:59:05 2000



From: Jim at ProSciTech :      jim-at-proscitech.com
Date: Thu, 26 Oct 2000 11:50:15 +1000
Subject: RE: Cleaning a Faraday Cage

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I expect that Faraday cage is actually a F cup - since it has an aperture on
top.
The F cage attracts low energy electrons towards the scintillator and the cup
is of course used to trap electrons and measure their current with a
picoampmeter for analysis.
We made our own when turning an SEM into a Probe about 20 years ago. Used were
an aperture holder, with a bit of machined carbon rod at the end and this was
capped with a 50um aperture. The carbon/aperture cup had to be insulated from
the holder. A fine wire and the aperture were held in place with a bit of
carbon dag.
Worked well, though insertion was not nearly as convenient as currently used
pneumatic F cups.

The relevance is that there is nothing "magical" about that device. The cup
most only conduct through the wire and meter to ground, but the cup must be
electrically conducting. I would not hesitate to remove the aperture, clean
this properly and replace it. A couple of small spots of dag would hold it in
place. Contamination could cause the insulating layer around the cup to conduct
and this would make the cup's reading useless. If you can measure with a good
meter any continuity between cup and holder itself, then you may need to
replace the insulating layer, which I expect is a bit of plastic sleeving; you
should be able to improvise something and that may be easier than cleaning that
plastic.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Thursday, October 26, 2000 2:26 AM, Frank Thomas
[SMTP:thomasf-at-AGC.BIO.NS.CA] wrote:
}
} Listers -
}
} Does anybody know a good way to clean a dirty Faraday cage? (I know, I
} shouldn't have let it get dirty in the first place....:-() I'm thinking
} sonification in soapy water with lots of rinsing afterwards, but I wonder
} if there are any drawbacks to this. I'm hesitant to use solvents because I
} don't know what "glue" was used to attach the little aperture on top, and
} it was way too expensive to screw up that way. I tried a puff of air from
} one of those little rubber squeeze-bulb thingies (whatever the hell they're
} called) but that just didn't do the trick.
} I don't really know what the contamination is but it's partially
} transparent in a 20KeV beam. Could be anything but metal, I guess.....
}
} F.C. Thomas
} MicroAnalysis Facility
} Bedford Institute of Oceanography
} Dartmouth, Nova Scotia
} Canada
} Geological Survey of Canada (Atlantic)
} Bedford Institute of Oceanography
} Dartmouth, Nova Scotia
} Canada B2Y 4A2




From daemon Thu Oct 26 06:19:44 2000



From: DrJohnRuss-at-aol.com
Date: Thu, 26 Oct 2000 07:08:45 EDT
Subject: Re: PhotoShop usage

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In a message dated 10/24/00 6:05:02 PM, mb-at-Soft-Imaging.com writes:

} I don't want to put down Photoshop, especially since we are producing a
} competing product, but I think this remark points to one problem that many
} "photo" applications have -- they do not treat the intensity as scientific
} data. I may be mistaken, but I think Photoshop is conceptually a
} "beautifying" software to make photos "look nicer" -- and it does a great
} job at this, but it may also be lacking in certain areas.

Michael is partly right - Photoshop does not include native capabilities for
calibration of intensity values for optical density, or the ability to
directly measure hue in color images, or to calibrate spatial distances in
microns, etc. It also has very limited image measurement capabilities. But it
has many advantages (particularly ease of use, consistency on various
computer platforms, a huge base of users so that few bugs survive, etc.) and
all of the other features that other responders to the original question have
pointed out, plus it is relatively inexpensive (particularly if you can take
advantage of the academic price). Many microscopists already have it, as
well. And the shortcomings are easily filled in using plug-ins. Those are the
reasons that my son and I decided to use it as the platform for writing
plug-ins to do scientific imaging. With the layers and actions capability in
the most recent versions (5.0 and 6.0), and the Tool Kit or Fovea plug-in
package (no Nestor, I won't make any further plug for the product - others
have already mentioned it anyway), the capabilities for scientific quality
image processing actually exceed those in many of the costly "commercial"
packages.

John C. Russ




From daemon Thu Oct 26 08:07:11 2000



From: tellis2-at-hallmark.com
Date: Thu, 26 Oct 2000 07:59:36 -0500
Subject: light microscope - vignette problem

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light microscope - vignette problem
Michael:

I had a similar problem on my metallurgical microscope when I first
installed a camera to it, at 25X and 50X

it would vignette but at higher magnifications it wouldn't . Kodak
suggested a higher C-mount magnification and

that took care of it.

This info is from the Kodak site:

"3.What is a C-Mount and why does it need to be 1.0X?
To accommodate the need for photo documentation, many microscopes
feature an additional photo port or tube that delivers an optical path
to a
camera. It is the C-Mount that adapts the microscope to the KODAK
MDS Universal Optical Adapter and MDS 290 digital camera.
C-Mounts of less than 1.0X magnification may cause images to vignette
(darkening around the edges of the image), and are therefore not
recommended. "

Terry Ellis
USA
Hallmark Cards Inc.





From daemon Thu Oct 26 08:47:52 2000



From: David_Bell-at-millipore.com
Date: Thu, 26 Oct 2000 09:38:22 -0400
Subject: Re: Fuji S1 digital cams on LMs

Contents Retrieved from Microscopy Listserver Archives
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John,

I'm one of the breath holders, as I'm currently waiting for my microscope
adapter to arrive. Got the camera and other goodies, but the adapter is on
back order. As I read the book, it seems you can't use all the automatic
bells and whistles unless you have a Nikon type D lens. Perhaps you need
to switch to aperture priority or shutter priority mode? Anyone else have
any ideas?



David Bell
Scientist
Electron Microscopy Lab
Millipore Corporation
80 Ashby Road
Bedford, MA 01730
(781) 533-2108





"John J.
Bozzola" To: microscopy-at-sparc5.microscopy.com
{bozzola-at-siu. cc:
edu} Subject: Fuji S1 digital cams on LMs

10/25/00
06:28 PM






------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


We just received our Fuji FinePix Pro S1 camera. Beautiful instrument!!

I have not had luck interfacing it the a light microscope since the
camera stubornly insists on having an autoNikkor lens attached to it.
The usual microscope adapters cause the camera to be uncommunicative
with the computer.

I KNOW that it can be interfaced with a LM and computer because I saw
it in action at M&M2000.

So, does anyone know how to do this?

Several people are holding their breaths on this one.....


John B.
--
####################################################################
John J. Bozzola, Ph.D.
Professor & Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
####################################################################







From daemon Thu Oct 26 09:52:09 2000



From: Linda Fox :      LFOX1-at-wpo.it.luc.edu
Date: Thu, 26 Oct 2000 09:48:12 -0500
Subject: subscribe

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subscribe



From daemon Thu Oct 26 13:37:56 2000



From: John Foust :      jfoust-at-threedee.com
Date: Thu, 26 Oct 2000 13:29:38 -0500
Subject: Re: microscope + coolpix camera problem

Contents Retrieved from Microscopy Listserver Archives
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At 07:43 AM 10/25/00 -0700, michael shaffer wrote:
} However, we were a bit disappointed when we tried to grab a
} digital image. The camera would vignette much more than the video
} display would indicate (there was actually no vignetting with the NTSC
} out) ... although the color is quite accurate.

In your second image, in the lower right corner for example,
I can see what I'd call true vignette, the sharp edge of
the lens tube. I'd guess you do see that small dark area
in the NTSC-out view. The rest of the ring of darkness could
be the drop-off in the light intensity of the microscope's output,
and perhaps an automatic exposure only properly exposed the
center of the view. Were you using manual or automatic
exposure settings? Did you try both methods? Where was
the zoom set?

I recently purchased an Olympus C-2500L. This is a true
SLR digital camera, roughly $1,000. I had high hopes of using it
in an afocal coupling manner with my Ortholux and a Dobsonian
telescope. In other words, I just wanted to point the camera
into the eyepiece and get a good view. There are also ~$150
adapters that will hold the camera in place in these
situations, like http://www.photosolve.com/xtendascope.asp .

I was very disappointed to learn that I should've bought a less
expensive camera non-SLR for this purpose - although I've heard
the Coolpix 8xx/9xx are suitable and otherwise nice, too. Those
with non-SLR lenses are much better at this sort of afocal
coupling. With the C-2500L, the front lens element doesn't
move when I zoom/tele. I can't avoid vignette-ing at all.

A 43 mm Plossl eyepiece might help on the telescope, and
I haven't had time to figure out which sort of microscope
eyepiece might help. I have other archived e-mails from a few
experts that I'd be glad to forward to anyone interested.

- John




From daemon Thu Oct 26 14:37:07 2000



From: COURYHOUSE-at-aol.com
Date: Thu, 26 Oct 2000 15:30:44 EDT
Subject: need namuals and prints on ..... RCA EMU3-F

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Good morning fellow listers, we have a patron that we are helping find a
set of RCA EMU3-F manuals and prints. any info leading to that would be
great. also if I could have the info on the folks that redo the florcesent
screen i will pass that along too ... thanks Ed Sharpe archivist for SMECC



From daemon Thu Oct 26 15:10:47 2000



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Thu, 26 Oct 2000 14:51:28 -0500
Subject: Re: Link ISIS v. 3 users

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We have a home-grown program that we wrote for the purpose of translating
batches of files into 8-bit TIFF. We then use other utilities to convert
those TIFF files to JPEG.

I use it only occasionally, and it may still have some bugs in it. But let
me check on its status and I can probably get it to you with some
instructions.

At 09:38 PM 10/24/2000 +0200, you wrote:

} Does anyone know of software that will do a batch conversion of the
} .IM_ image files captured using Link ISIS v. 3 Autobeam, to a standard
} format (eg. pcx, jpg, etc)?
}
} Fiona
} ____________________
} Dr Fiona Graham
} EM Unit,
} University of Natal, Durban
} South Africa

----------------------
Warren E. Straszheim
Materials Analysis and Research Lab
Iowa State University
23 Town Engineering
Ames IA, 50011-3232

Ph: 515-294-8187
FAX: 515-294-4563

E-Mail: wesaia-at-iastate.edu
Web: www.marl.iastate.edu

Scanning electron microscopy, x-ray analysis, and image analysis of materials
Computer applications and networking



From daemon Thu Oct 26 15:37:08 2000



From: John J. Bozzola :      bozzola-at-siu.edu
Date: Thu, 26 Oct 2000 15:31:35 -0500
Subject: Fuji S1: problem solved

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I just got off the phone with the Fuji representative (thank you
Gary) who worked with me to solve the interface problem with the Fuji
S1 camera. Turns out that the software had to be set up properly. Now
the system works without a hitch. The images are FANTASTIC.

We had originally purchased the camera for copystand, macro
photography but I suspect that the S1 will be spending most of its
life on the light microscope from this point on. It is supposed to
work even on fluorescent images -- so I am looking forward to
checking that out too.

Thanks also to Matt Irwin from ElectroImage who called and also
helped point me in the right direction.

This camera is really wonderful!!!!


JB
--
####################################################################
John J. Bozzola, Ph.D.
Professor & Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
####################################################################



From daemon Thu Oct 26 17:59:15 2000



From: Vachik Hacopian :      vhacopian-at-wellesley.edu
Date: Thu, 26 Oct 2000 18:54:14 -0400
Subject: parts for Zeiss EM 9S 2

Contents Retrieved from Microscopy Listserver Archives
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I have a Zeiss EM 9S 2, vintage 1975, which I would like to keep running
for as long as possible. Parts are very difficult to obtain. If any of
you are considering discarding one of these instruments, please get in
touch with me. I'd be happy to take it off your hands. Thanks.

Vachik Hacopian





From daemon Thu Oct 26 19:59:53 2000



From: patricia :      pmreis-at-morango.esb.ucp.pt
Date: Thu, 26 Oct 2000 19:53:27 -0500
Subject: Low-Vacuum SEM

Contents Retrieved from Microscopy Listserver Archives
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Good morning everyone, I' m looking for specimen preparation to
Low-Vacuum SEM observation. I work with a Jeol, JSM-5600LV and I' d
like to see lactic microorganisms and microalgae with that SEM. Does
anyone Know "SEMpore" which is a registered mark of Jeol A. B. used for
some authors to observe Cryptosporidium using a LV SEM? I' m hoping that
someone can give me some advice to specimen preparation. Regards,
Patrícia. _____________________________________________
Patrícia João Reis
Escola Superior de Biotecnologia
Rua Dr. António Bernardino de Almeida
4200 - 072 Porto Portugal Telef. +351 225580044
Fax. +351 22 5090351
E-mail: pmreis-at-morango.esb.ucp.pt





From daemon Thu Oct 26 19:59:55 2000



From: Bankman, Isaac N. :      Isaac.Bankman-at-jhuapl.edu
Date: Thu, 26 Oct 2000 19:54:35 -0500
Subject: reflectance microscopy

Contents Retrieved from Microscopy Listserver Archives
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I am planning to use reflectance microscopy on live cells and I am looking
for bathing solutions that can increase the reflectance from the cell
membrane without harming the cells. Any suggestions ? Are there such
products out there ?
Thanks,

Isaac Bankman
Johns Hopkins University
Applied Physics Laboratory






From daemon Fri Oct 27 10:07:35 2000



From: White, Woody N. :      nwwhite-at-mcdermott.com
Date: Fri, 27 Oct 2000 09:47:46 -0500
Subject: RE: Link ISIS v. 3 users

Contents Retrieved from Microscopy Listserver Archives
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Warren et al,

FWIW: When IXRF images are exported to TIFF, the code allows a max array of
1024. Larger images are reduced to that on export. Sometimes a high
resolution / low mag image is useful to have. I have had limited success
using photoshop to read the native files. I change the file type to .raw
then open w/photoshop. PShop will try to "guess" the header size. Seems to
work well with 4Kx4K arrays, but fails with 2K square. Since I'm after an
image allowing me to digitally zoom in and roam around, this is not sooo
bad.

Woody White
McDermott Technology, Inc
Lynchburg Research Center

Me: http://home.att.net/~woody.white


{ {SNIP} }
}
} We have a home-grown program that we wrote for the purpose of
} translating
} batches of files into 8-bit TIFF. We then use other utilities
} to convert
} those TIFF files to JPEG.





From daemon Fri Oct 27 17:23:07 2000



From: Wil Bigelow :      bigelow-at-engin.umich.edu
Date: Fri, 27 Oct 2000 14:42:01 -0400
Subject: RE: Cleaning Fy Cup

Contents Retrieved from Microscopy Listserver Archives
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I have very good luck cleaning things that go into electron optical
instruments using Tiles Soap Scum Remover. This is a mixture of high
powered detergents combined with a organic solvent (diethylene glycol butyl
ether). I just scrub parts with this thoroughly, or treat them with it
ultrasonically, then rinse them thoroughly with hot running water, and
finish by rinsing with reagent grade isopropyl alcohol and blowing off the
residual alcohol with a duster. I've been able to remove pump oils,
machining oils, and even silicone grease in this way, and have had no
problems when parts so cleaned are later put in a high vacuum system.

Wilbur C. Bigelow, Prof. Emeritus
Materials Sci. & Engr., University of Michigan
Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu;
Fx:734-763-4788; Ph:734-662-5237





From daemon Fri Oct 27 17:23:07 2000



From: Wil Bigelow :      bigelow-at-engin.umich.edu
Date: Fri, 27 Oct 2000 14:42:01 -0400
Subject: RE: Cleaning Fy Cup

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I thought I'd get back to you all and let you know the problem of
vignetting was not a question of "zoom" (although you cannot zoom out
and not see true vignetting because of the tube). The problem was
apparently my CP800 and its aperture adjustment for proper exposure.

Thanx to those who suggested I turn off the "flash" which sets a
different aperture priority, ... and fixing the focus at infinity. I
can now take photos similar to what was posted previously and not see
vignetting ... 'cept very slightly which suggests I ought to increase
the zoom only slightly, but might be corrected properly with the
larger CP990's CCD.

However, I still experience similar vignetting for very bright images
.. the CP800 will still close down the aperture for compensation.
However, this occurs (for example) if I take picture with crossed
polars, and then want the same image without crossed polars ... but I
could fix this with a neutral density filter.

I haven't yet had a chance to play with a CP950 or 990, but
presumably these "better" cameras come with more features ... possibly
a "aperture priority" mode which would lock-out the problem.

I cannot yet post images until I've had time to download to my home
computer ... possibly next week.

cheerios, =shAf= :o)

{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {}
Michael Shaffer, R.A. - mshaf-at-darkwing.uoregon.edu
Geological Science's Electron Probe Facility - University of Oregon
http://epmalab.uoregon.edu/




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I have very good luck cleaning things that go into electron optical
instruments using Tiles Soap Scum Remover. This is a mixture of high
powered detergents combined with a organic solvent (diethylene glycol butyl
ether). I just scrub parts with this thoroughly, or treat them with it
ultrasonically, then rinse them thoroughly with hot running water, and
finish by rinsing with reagent grade isopropyl alcohol and blowing off the
residual alcohol with a duster. I've been able to remove pump oils,
machining oils, and even silicone grease in this way, and have had no
problems when parts so cleaned are later put in a high vacuum system.

Wilbur C. Bigelow, Prof. Emeritus
Materials Sci. & Engr., University of Michigan
Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu;
Fx:734-763-4788; Ph:734-662-5237





From daemon Fri Oct 27 18:58:54 2000



From: Stuart McKernan :      mckernan-at-cems.umn.edu
Date: Fri, 27 Oct 2000 17:25:08 -0500 (CDT)
Subject: Minnesota Society meeting

Contents Retrieved from Microscopy Listserver Archives
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The next meeting of the Minnesota Microscopy Society will be on Thursday,
November 9, 2000

The theme of the meetng is "Everything you wanted to know about SPM but were
afraid to ask"; A talk to be given by Chuck Mooney, SPM Assistant Product
Manager, JEOL USA, Inc.

Abstract:
The first scanning probe microscope (SPM), the scanning tunneling
microscope, was introduced to the world in 1981 as a frightfully complex
laboratory experiment where conductors could be probed at the atomic scale.
Five years later, the Nobel Prize in Physics was awarded to its inventors, even
as the basic technique was expanded to include probing insulators with the
invention of the atomic force microscope (AFM). Since that time, many new
methods have been added to the general field of scanning probe microscopy that
allow for the probing of all manner of samples and forces. Recent breakthroughs
have allowed the measurement of the strength of bonds between atomic species as
well as the potential for 3-D sub-surface imaging and spectroscopy of atomic
species near the sample surface. Many research laboratories have added scanning
probe microscopy techniques to their analytical arsenals aided by the
introduction of commercially available instruments.
Unfortunately, the strengths and weaknesses of SPM remain a mystery to many
who could benefit from a better understanding of both the techniques that are
routine and those that are difficult, the relative levels of information that
can be extracted from those techniques, and the types of samples that are
suitable for most SPMs. In the time allowed, an introduction to the various SPM
techniques will be given, applications examples shown, as well as providing
insight into the future of probe microscopy.

Location: Medtronic, Inc., 6700 Shingle Creek Parkway, Brooklyn Center

Program: 6:00-7:00 PM Dinner
7:30-7:45 PM Business meeting
7:45-8:30 PM Speaker

Dinner: New King Buffet, 5927 John Martin Drive, Brooklyn Center

The New King Buffet is a full menu Chinese buffet. Cost is $10.00 per person.

We will first meet at the New King Buffet for dinner.
After dinner, we will reconvene at Medtronic for the business and technical
meetings.

For reservations contact Mike Coscio by Friday, November 3: (763-514-1331, or
mike.coscio-at-medtronic.com).


__________________
Stuart McKernan stuartm-at-tc.umn.edu
Office:(612) 626-7594
IT Characterization Facility, University of Minnesota Desk:(612) 624-6009
100 Union Street S. E., Minneapolis, MN 55455 NEW-} Fax:(612) 625-5368




From daemon Fri Oct 27 18:58:54 2000



From: michael shaffer :      epmalab-at-darkwing.uoregon.edu
Date: Fri, 27 Oct 2000 12:34:26 -0700
Subject: RE: microscope+coolpix (my findings)

Contents Retrieved from Microscopy Listserver Archives
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Dear listers,

I'd like more CPD advice again. I just had a very bad run on our new Pelco
CPD2--stuff came out visibly wet! And the o ring on the door is
already stretched out of shape. It's the last straw for my supervisor,
we're sending it back. But now we would like to know what to get. Does
anyone have a Tousimis Samdri-type drier? How is it? Any other *users*
with recommendations who want to tell us why they like their CPDs? Mail
from company reps, while not solicited, will be nonetheless noted.

Thanks for the help from those who wrote last time, and thanks in advance
to those with responses.

Pauline Yu
splene-at-pw.usda.gov
Microscopist Technician
USDA-ARS-WRRC



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I thought I'd get back to you all and let you know the problem of
vignetting was not a question of "zoom" (although you cannot zoom out
and not see true vignetting because of the tube). The problem was
apparently my CP800 and its aperture adjustment for proper exposure.

Thanx to those who suggested I turn off the "flash" which sets a
different aperture priority, ... and fixing the focus at infinity. I
can now take photos similar to what was posted previously and not see
vignetting ... 'cept very slightly which suggests I ought to increase
the zoom only slightly, but might be corrected properly with the
larger CP990's CCD.

However, I still experience similar vignetting for very bright images
.. the CP800 will still close down the aperture for compensation.
However, this occurs (for example) if I take picture with crossed
polars, and then want the same image without crossed polars ... but I
could fix this with a neutral density filter.

I haven't yet had a chance to play with a CP950 or 990, but
presumably these "better" cameras come with more features ... possibly
a "aperture priority" mode which would lock-out the problem.

I cannot yet post images until I've had time to download to my home
computer ... possibly next week.

cheerios, =shAf= :o)

{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {}
Michael Shaffer, R.A. - mshaf-at-darkwing.uoregon.edu
Geological Science's Electron Probe Facility - University of Oregon
http://epmalab.uoregon.edu/





From daemon Fri Oct 27 20:41:23 2000



From: Al Coritz :      al-at-boeckeler.com
Date: Fri, 27 Oct 2000 18:36:08 -0700
Subject: subscribe

Contents Retrieved from Microscopy Listserver Archives
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Al Coritz
Sales & Service Manager
RMC-Boeckeler Instruments
4650 S. Butterfield Dr.
Tucson, AZ 85714
Voice: 520-745-0001
Cell: 520-465-3598
Fax: 520-745-0004
Email:Al-at-Boeckeler.com
Website:RMCProducts.com




From daemon Sat Oct 28 07:57:11 2000



From: Chris Jeffree :      cjeffree-at-srv0.bio.ed.ac.uk
Date: Sat, 28 Oct 2000 13:41:10 +0100
Subject: Re: more CPD questions

Contents Retrieved from Microscopy Listserver Archives
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Pauline

Before damning the Pelco, are you certain your methods are good?
I do not know how experienced you are with CPD, so forgive me if
you have thought of the following already. However, CPDs are very
simple systems and most problems arise from operator errors, or
failure to understand the basic principles


If the specimens are visibly wet then they are, presumably, wet
either with the transition fluid or water - What fluid are you using?
amyl acetate, acetone, ethanol, or something else? Can you smell
it? Is the wetness of the specimen with the transition fluid or with
water? If water, the specimens are not properly solvent-dehydrated.
If transitional solvent, the sovent has not been removed by the CO2


The immersion in liquid CO2 should very quickly remove the
transition fluid unless

1) the specimens were not fully immersed in liquid CO2, but only in
gas - you must either use the dip-tube type of CO2 cylinder, or
invert a non-dip type cylinder to obtain a supply of liquid CO2 to the
CPD chamber. Remember that the dip-tube type must actually
contain a liquid level - for some time after the available liquid is
exhausted the cylinder will continue to supply gas, but you cannot
do CPD with CO2 gas

2) insufficient liquid CO2 flushing took place, so some of the
transition fluid remained in the chamber: flush for longer, or for
short bursts with soak-time between

3) The specimens were very large, and insufficient CO2 rinsing time
was allowed - see 2

4) The specimens were still wet with water: use more solvent-drying
time with drier solvents


I think I am right in saying that the dehydration and transition fluid
stages are where your problems should be sought, and that errors
of method or hardware faults in or leading up to the actual critical
point stage would not lead to wet specimens if the earlier procedures
were correctly followed.


Chris


} Dear listers,

{color} {param} 0000,0000,0000 {/param} }

} I'd like more CPD advice again. I just had a very bad run on our new Pelco

} CPD2--stuff came out visibly wet! And the o ring on the door is

} already stretched out of shape. It's the last straw for my supervisor,

} we're sending it back. But now we would like to know what to get. Does

} anyone have a Tousimis Samdri-type drier? How is it? Any other *users*

} with recommendations who want to tell us why they like their CPDs? Mail

} from company reps, while not solicited, will be nonetheless noted.

}

} Thanks for the help from those who wrote last time, and thanks in advance

} to those with responses.

}

} Pauline Yu

} splene-at-pw.usda.gov

} Microscopist Technician

} USDA-ARS-WRRC

}

}



{nofill}
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Dr Chris Jeffree
University of Edinburgh
Biological Sciences EM Facility
Daniel Rutherford Building
King's Buildings EDINBURGH EH9 3JH
Tel: +44 (0) 131 650 5345
FAX: +44 (0) 131 650 6563

Inveresk Cottage, 26 Carberry Road,
Inveresk, Musselburgh, Midlothian EH21 8PR, UK
Tel: +44 (0) 131 665 6062 / Mobile 07710 585 401
FAX: +44 (0) 131 653 6248
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~



From daemon Sat Oct 28 14:14:42 2000



From: Rosemary Walsh :      rw9-at-psu.edu
Date: Sat, 28 Oct 2000 15:12:09 -0400
Subject: Re: more CPD questions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Pauline,
I agree with Chris Jeffree on two important points:
1. use bone-dry liquid carbon dioxide with a dip tube
2. use several steps of 100% ethanol (I recommend that all using our older
Polaron E3000 use an unopened pint bottle of 100% ethanol in the last two
ethanol steps. This is esp. important during the summer months when it is
humid here)
and would add the following tips
--when dehydrating plant samples esp. vegetative or reproductive
meristems, leave samples overnight in the third 100% ethanol and change
again the next day before transferring to the CPD
--when dehydrating drosophila or other whole insects, add several
overnights in 100% ethanol
--after filling the pressure chamber with the liquid carbon dioxide,
check to see a meniscus, an indication that you have liquid carbon dioxide
(for safe viewing, it's recommended that one do this on our system by
directing a flashlight into the chamber while viewing the chamber via a
mirror placed opposite to the chamber window) -- if the temperature of your
chamber is higher than 19C, the carbon dioxide will fill the chamber and
the pressure gauge will read 800psi but what you may have is vapor
--use a flushing step just after filling with liquid carbon dioxide,
i.e., with the inlet open, open the drain valve and monitor the drain line
(at first, you'll see liquid (ethanol), a slush (ethanol/CO2) and then
white powder (dry ice) -- many of our users place samples in the
polypropylene
specimen carriers which hold the ethanol when capped and we find it
advantageous to use this step each time so that our subsequent exchanges
are replacing the ethanol within tissues or organisms
--any problems we had with gaskets were solved by purchasing those
supplied by Energy Beam Sciences.
Rosemary




From daemon Mon Oct 30 00:55:17 2000



From: Robin Cross :      r.cross-at-ru.ac.za
Date: Mon, 30 Oct 2000 08:30:35 +0200
Subject: Re: more CPD questions

Contents Retrieved from Microscopy Listserver Archives
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Dear Pauline

} I'd like more CPD advice again.

Try staying simple. We have used two Polaron E3000 CP Dryers
for at least 25 years and they have been very reliable. Some
gaskets and seals have had to be changed but other than that we
have had no problems. They do not have the automated features
that some of the others have but they produce good results.

I think they are sold in the US by SPI, and perhaps other vendors
too.

Good luck.

Rob


Robin H Cross
Director : EM Unit, Rhodes University, Grahamstown, South Africa
R.Cross-at-ru.ac.za
tel: +27 46 603 8168 - fax: +27 46 622 4377
http://www.ru.ac.za/emu/index.htm

** remember that ICEM-15 in 2002 is in Durban, South Africa **



From daemon Mon Oct 30 00:55:17 2000



From: Marta Daglow :      daglowcg-at-earthlink.net
Date: Sun, 29 Oct 2000 22:36:54 -0700
Subject: STORMFRONT JOBS (4024bb82)

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Right now Stormfront Studios is adding to its staff and looking for
people to create Next Gen Games for the X-BOX and PS2. We're looking
for an Entry Programmer, AI Programmer, Sr. Programmer, Lead Programmer,
Development Director, Sr. 3D Max Artist, 3D Animator and/or Art
Director. Interested or know of anyone who may be interested? Please
let me know if you can help us out - we need your talent!

Thanks,
Marta Daglow
EMAIL: mdaglow-at-earthlink.net
WEB: www.stormfront.com
San Rafael, CA 94903




From daemon Mon Oct 30 03:30:53 2000



From: Evgeny Zhikharev :      enzh-at-radio-msu.net
Date: Mon, 30 Oct 2000 12:23:01 +0300 (MSK)
Subject: SEM CamScan4 upgrade for low vacuum

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Dear colleagues,
I would like to carry out experiment of irradiating an object with
electrons in presence of gas media (about 1 torr) and intend to use
CamScan4 SEM for
this purpose. This device doesn't contain low vacuum accessories, so I
hope to make them by myself. Could you tell me
which are the tipical holes sizes in the differential pumping apertures,
the distance between them and expected pressure increasing in
diff. pump entrance.
Thanks in advance.
Eugeny Jikharev,
Physics & Technology Institute of Russian Academy of Sciences,
Moscow.








From daemon Mon Oct 30 05:07:14 2000



From: Ruediger Kuenzler :      kuenzler-at-dibis.de
Date: Mon, 30 Oct 2000 11:58:26 +0100
Subject: Film / Plate Holders for TEM

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Dear all,

I am looking for some TEM negative film holders for Philips/FEI
microscopes.
These are holders with the format 3,25 x 4’’ and have two components.
The holder itself and the insertion (for loading film and imaging
plates).
Placing an order in Germany it takes 6 months to get them – quite a long
time.
Is anybody out there who knows an other source to get the mentioned
holders
faster or has someone any used material available ?


Ruediger Kuenzler
--
DIBIS
Digital Biomedical Imaging Systems AG
Gewerbestraße 11; D-75217 Birkenfeld
Tel.: +49 (0)7082 940639
Fax : +49 (0)7082 940076
E-Mail: contact-at-dibis.de
E-Mail: kuenzler-at-dibis.de
Internet: http://www.dibis.de





From daemon Mon Oct 30 09:09:46 2000



From: Brandon H. Poe :      bpoe-at-ravenbrand.com
Date: Mon, 30 Oct 2000 09:31:42 -0800
Subject: TEMPORARY RESEARCH SUPPORT POSITION AVAILABLE

Contents Retrieved from Microscopy Listserver Archives
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Our laboratory is looking for an assistant to help prepare grants and publications.
Below is the text of an announcement written for our HR department. In the past this
position has been held by recent college graduates preparing applications for medical
or graduate school or by trained research personnel interested in re-entering the
workforce.
-Brandon Poe, Ph.D.

===

We are looking for someone to fill a full- or part-time, temporary job, helping our
neuroscience research laboratory to prepare reports on our research for publications
and grant applications. This will require using image analysis software (e.g. Adobe
Photoshop) and some darkroom work. Learning specific skills on the job is acceptable,
but some familiarity with computers and illustration techniques will be preferred. We
also need help with literature searches (w/ Ovid & Medline) and running a
computer-based bibliography program (i.e. Papyrus). For this work, a background in
college-level biology would be preferred. Time permitting, participation in
quantitative data analyses would be an option, and a familiarity with statistical
methods would be desirable. This job is available immediately for a period of 6-9
months. It would be suitable for a part-time college science major, a college
graduate preparing for professional school or for a return to the job market after
interruption of a career in science.

Contact: D. Kent Morest, M.D.
Professor, Department of Neuroscience
Director, Center for Neurological Sciences
The University of Connecticut Health Center
263 Farmington Avenue
Farmington, CT 06030-3401
tel: 860-679-2645
fax: 860-679-8766
email: kent-at-neuron.uchc.edu
web: http//www3.uchc.edu/~kent

--
Brandon H. Poe, Ph.D.
Department of Neuroscience
University of Connecticut
Health Center
Farmington, CT 06030
email: bpoe-at-ravenbrand.com





From daemon Mon Oct 30 09:34:24 2000



From: Leona Cohen-Gould :      lcgould-at-mail.med.cornell.edu
Date: Mon, 30 Oct 2000 10:32:13 -0500
Subject: Re: more CPD questions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


}
Pauline,
Here's my 2-cents....
}
I just had a very bad run on our new Pelco CPD2--stuff came out visibly wet!

} } } } } } } It sounds as though you had not fully purged the transitional fluid
} } } } } } } and/or you did not have a sufficient liquid CO2 level before you
} } } } } } } heated.

And the o ring on the door is already stretched out of shape.

} } } } This sounds like you are getting the O-ring wet with your transitional
} } } } fluid (ethanol?) when you are loading the chamber.

These problems will persist unless care is taken when loading samples and
doing the "run". CPD is not something you can do well without paying close
attention to each step no matter whose unit you use.

As a "veteran" of some of these same problems, with a different brand unit
than yours, I speak from painful experience!

Good luck,
Lee

Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175





From daemon Mon Oct 30 09:37:50 2000



From: michael shaffer :      epmalab-at-darkwing.uoregon.edu
Date: Mon, 30 Oct 2000 07:35:48 -0800
Subject: RE: microscope+coolpix (my findings)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have put images up on my wwwsite for your examination.
Again, the Coolpix 800 was mounted on a 1X C-mount with the
Nikon CP adapter.

A full-size crossed polar image of biotite which
exhibits only minor vignetting:
http://darkwing.uoregon.edu/~mshaf/dogsci/biotite-x.jpg

... the same image reduced in size:
http://darkwing.uoregon.edu/~mshaf/dogsci/biotite-x-s.jpg

... the same biotite image ... uncrossed polars
(no analyzer) ... which exhibits the problem vignetting
because the CP800 stopped down its aperture:
http://darkwing.uoregon.edu/~mshaf/dogsci/biotite-nx-s.jpg

A interference figure (calcite):
http://darkwing.uoregon.edu/~mshaf/dogsci/bertrand.jpg

I had previously implied the CP800 would be a very
low cost alternative for putting a "pro-sumer" digital
camera on a microscope ...~US$800. Apparently I was wrong
.. only under some lighting conditions will it work correctly.
You do need one of the CP9xx series and use their "aperture
priority" mode in most cases of illumination.

cheerios, =shAf= :o)

{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {}
Michael Shaffer, R.A. - mshaf-at-darkwing.uoregon.edu
Geological Science's Electron Probe Facility - University of Oregon
http://epmalab.uoregon.edu/




From daemon Mon Oct 30 13:09:20 2000



From: Jill Verlander Reed :      verlaj-at-medicine.ufl.edu
Date: Mon, 30 Oct 2000 14:03:24 -0500
Subject: CPD use

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Pauline -
A user had a similar problem on a recent run using our stalwart
Tousimis Samdri unit that has been in use for at least 20 years. The
problem was due to an operator error - the purge valve was accidentally
left closed so that the CO2 flow was too little or non-existent. The
next run was perfect.

Good luck. Jill

Jill Verlander Reed, D.V.M.
Associate Scientist
Director, College of Medicine Electron Microscopy Core Facility
University of Florida
P.O. Box 100215
Gainesville, FL 32610
verlaj-at-medicine.ufl.edu
Phone: (352) 846-0820
Fax: (352) 846-3299



From daemon Mon Oct 30 13:27:37 2000



From: ed_bachmann-at-unc.edu (Ed Bachmann)
Date: Mon, 30 Oct 2000 14:29:10 -0500 (Eastern Standard Time)
Subject: Steve R. Poe, USDA, APHIS - email address?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Sorry to bother the list over this, but I received an email from Steve Poe,
USDA, APHIS, responding to a question posted to the microscopy list. I tried to
reply to the address given in his message:

Steve.R.Poe%aphisn1-at-hq.usda.gov

but my message was returned with a "user unknown" error.

If anyone has an email address for Steve other than the one above, please let me
know.

Thanks,
Ed

Ed Bachmann
Odum Institute for Research in Social Science
Manning Hall CB 3355
University of North Carolina at Chapel Hill
Chapel Hill, NC 27599-3355
(919) 962-0512




From daemon Mon Oct 30 13:30:04 2000



From: Art Rosenblatt :      arsiclax-at-pacbell.net
Date: Mon, 30 Oct 2000 11:09:30 -0800
Subject: JOB POSTING

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Sales person needed for Los Angeles area. Direct sales of microscope and
digital imaging systems.
Please replay to my e-mail arsiclax-at-pacbell.net




From daemon Mon Oct 30 14:27:18 2000



From: McLean, Dorrance :      dmclea-at-sandia.gov
Date: Mon, 30 Oct 2000 13:23:21 -0700
Subject: Sorvall Mictrome Info

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear All,

Thanks for the replies to my question about thin films grown on PMMA. My
original attempts to do cross sections in the PIPs have not yielded much
success. Mainly because my samples are highly allergic to both heat and
acetone so normal things like low temperature wax on the dimpler are out ...
my next plan was to use the microtome.

However, my attempts at reviving an old (1986) unused (no one here knows how
long ago) Sorvall Microtome have been for naught! I finally located (I
think) all the blown fuses, burnt out light bulbs and loose wires, but I
don't think the cutting arm is retracting after the down stroke. The block
face seems to be rubbing against the back of the knife on the return
(upward) stroke and dragging water and cut sections from the boat up with
it.

Also, all my attempts at locating the Sorvall Company have not proven
fruitful. I get all kinds of web addresses, all in German (not my native
tongue) and they all take me to the same error message (Maybe this whole
project is just not supposed to happen). So if any one can help with an
good address or a service number for Sorvall, I would say prayers in your
name to the electron gods!

Thanks in advance for all your help.
Dorrance.





From daemon Mon Oct 30 15:20:19 2000



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Tue, 31 Oct 2000 10:22:10 GMT+1200
Subject: Multilayer crystals for JEOL 840

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi, Listers

Does anyone know if there is available for the JEOL 840/733 spectros
a multilayer which covers O to P, same as the TAP?

And about how much they cost?

I'm having some difficulty getting any real info from Osmic.

Are there any other suppliers?


cheers

rtch

Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand



From daemon Mon Oct 30 16:12:05 2000



From: Leona Cohen-Gould :      lcgould-at-mail.med.cornell.edu
Date: Mon, 30 Oct 2000 17:08:53 -0500
Subject: Re: Sorvall Mictrome Info

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} Also, all my attempts at locating the Sorvall Company have not proven
} fruitful. I get all kinds of web addresses, all in German (not my native
} tongue) and they all take me to the same error message (Maybe this whole
} project is just not supposed to happen). So if any one can help with an
} good address or a service number for Sorvall, I would say prayers in your
} name to the electron gods!
}
} Thanks in advance for all your help.
} Dorrance.

***************
Hi Dorrance,

Sorvall was bought years ago by a company called RMC (Research
Manufacturing Company), which has just become part of Boeckeler
Instruments in Tucson, AZ.

Boeckeler Instruments, Inc.
4650 South Butterfield Drive
Tucson, AZ 85714

Tel: (520) 745-0001 Fax: (520) 745-0004

email: info-at-boeckeler.com


I'm not sure that they can help you much, since RMC stopped supporting many
of the older instruments (Parts are no longer available). Give them a
call though, you never know....

Lee

(I have no financial interest in Sorvall, RMC or Boeckeler)


Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175





From daemon Mon Oct 30 16:47:11 2000



From: William A. Muller :      wamuller-at-med.cornell.edu
Date: Mon, 30 Oct 2000 17:41:34 -0500
Subject: Postdoctoral Position Available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Colleagues:
I have made great contacts through this listserver in the past and hope
that I can again this time. I am in search of a postdoctoral fellow to
continue a program of research using modern optical imaging methods to
follow membrane trafficking in vascular endothelial cells. It is a very
exciting project that cuts across the fields of cell biology, immunology,
and pathology, and uses techniques from standard transmission EM to
tracking GFP-labeled proteins. If you have the right background and are
interested, or know of a colleague who fits these criteria, please get in
touch with me.
The following is the text of an ad that appeared in the August 10 issue of
Nature and the September issue of Nature Cell Biology:

Postdoctoral position in Membrane Trafficking
Postdoctoral position open to study membrane trafficking in
endothelial cells. The ideal candidate would have experience studying
regulated membrane trafficking at the cellular and molecular levels using
biochemical, microscopic, and molecular biology approaches. Experience with
modern imaging systems is critical. Experience with endothelial cells is a
definite "plus", but not necessary. The candidate should demonstrate
excellent communication skills in English, be able to work independently,
as well as to interact with a lively group of productive investigators
studying leukocyte-endothelial cell interactions. We are based at the
Weill Medical College of Cornell University in the nicest and safest area
of New York City. We interact extensively with investigators Weill as well
as at The Rockefeller University and Memorial Sloan-Kettering Cancer
Center, which are both just across the street. The position is
fully-funded and available immediately.

Submit CV and names (and contact numbers) of three references to:

William A. Muller, MD, PhD
Department of Pathology and Program in Immunology
Box 69
Weill Medical College of Cornell University
1300 York Avenue
New York, NY 10021
e-mail: wamuller-at-med.cornell.edu


Thank you very much for your help.

Sincerely,
Bill Muller


William A. Muller, MD, PhD
Associate Professor of Pathology
Weill Medical College of Cornell University
1300 York Avenue
New York, NY 10021

Phone: (212) 746-6487
Fax: (212) 746-6991
e-mail: wamuller-at-med.cornell.edu



From daemon Mon Oct 30 21:39:24 2000



From: Adam Papworth :      ajp5-at-lehigh.edu
Date: Mon, 30 Oct 2000 23:36:10 -0400
Subject: unsubscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Unsubscribe.
--
Dr Adam Papworth
Materials Research Centre
Lehigh University
5 East Packer Avenue
Bethlehem PA 18015 USA

Phone
(Work) (610) 758 6879
(Home) (610) 758 1829
(FAX) (610) 758 4244
e-mail ajp5-at-lehigh.edu



From daemon Tue Oct 31 01:47:30 2000



From: O. O. Ilori :      sojilori-at-oauife.edu.ng
Date: Tue, 31 Oct 2000 08:31:14 +0100 (WAT)
Subject: Emulsion.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Is it possible to view an emulsion under a SEM? By emulsion I mean
particles suspended in a matrix.
If possible, how?


Mr. O. O. ILORI
DEPARTMENT OF ELECTRONIC/ELECTRICAL ENGINEERING
OBAFEMI AWOLOWO UNIVERSITY,
ILE-IFE, OSUN STATE
NIGERIA.

email: sojilori-at-oauife.edu.ng





From daemon Tue Oct 31 03:34:03 2000



From: Alan Bright :      bright-at-dial.pipex.com
Date: Tue, 31 Oct 2000 09:21:26 -0000
Subject: Sorvall Mictrome Info

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Dorrance,

Why do you not try to section your thin film samples in a cryostat, we have
manufactured and supplied many for this application with great success.

Best Regards

Alan Bright

Bright Instrument Co.Ltd.
St Margaret's Way
Huntingdon
PE18 6EB
England

Tel No:+44 (0)1480 454528
Fax No:+44 (0)1480 456031
Email: AlanBright-at-brightinstruments.com
Web Site: www.brightinstruments.com



-----Original Message-----
} From: McLean, Dorrance [mailto:dmclea-at-sandia.gov]
Sent: 30 October 2000 20:23
To: 'microscopy'


Dear All,

Thanks for the replies to my question about thin films grown on PMMA. My
original attempts to do cross sections in the PIPs have not yielded much
success. Mainly because my samples are highly allergic to both heat and
acetone so normal things like low temperature wax on the dimpler are out ...
my next plan was to use the microtome.

However, my attempts at reviving an old (1986) unused (no one here knows how
long ago) Sorvall Microtome have been for naught! I finally located (I
think) all the blown fuses, burnt out light bulbs and loose wires, but I
don't think the cutting arm is retracting after the down stroke. The block
face seems to be rubbing against the back of the knife on the return
(upward) stroke and dragging water and cut sections from the boat up with
it.

Also, all my attempts at locating the Sorvall Company have not proven
fruitful. I get all kinds of web addresses, all in German (not my native
tongue) and they all take me to the same error message (Maybe this whole
project is just not supposed to happen). So if any one can help with an
good address or a service number for Sorvall, I would say prayers in your
name to the electron gods!

Thanks in advance for all your help.
Dorrance.






From daemon Tue Oct 31 05:57:46 2000



From: Dmitri :      kolmykow-at-zeiss-msk.ru
Date: Tue, 31 Oct 2000 14:34:49 +0300
Subject: Re: SEM CamScan4 upgrade for low vacuum

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Evgeny,
1. Could you please tell more precise what signals you are going to use and
what kind of objects you are going to investigate?
2. It depends on your vac system (with or without IGP, number of pirani
gauges) but you will have to change some thresholds in the VAC electronics.
3.If you intend to insert the aperture in the objective lens (typical size
600-800micron) and use a BSD as a detector you should change:
READY threshold in a high vac measuring (gray box behind the column)
PREVAC thresholds in the VAC PCB
4. Anyway I wouldn't recommend to do this way because you will get problems
with vacuum system - it's very sensitive.

Good luck,

Dmitri


Evgeny Zhikharev wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Dear colleagues,
} I would like to carry out experiment of irradiating an object with
} electrons in presence of gas media (about 1 torr) and intend to use
} CamScan4 SEM for
} this purpose. This device doesn't contain low vacuum accessories, so I
} hope to make them by myself. Could you tell me
} which are the tipical holes sizes in the differential pumping apertures,
} the distance between them and expected pressure increasing in
} diff. pump entrance.
} Thanks in advance.
} Eugeny Jikharev,
} Physics & Technology Institute of Russian Academy of Sciences,
} Moscow.
}
}







From daemon Tue Oct 31 05:57:47 2000



From: Ken Converse :      qualityimages-at-netrax.net
Date: Tue, 31 Oct 2000 07:51:44 -0800
Subject: Re: Multilayer crystals for JEOL 840

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ritchie Sims wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hi, Listers
}
} Does anyone know if there is available for the JEOL 840/733 spectros
} a multilayer which covers O to P, same as the TAP?
}
} And about how much they cost?
}
} I'm having some difficulty getting any real info from Osmic.
}
} Are there any other suppliers?
}
} cheers
}
} rtch
}
} Ritchie Sims Phone : 64 9 3737599 ext 7713
} Department of Geology Fax : 64 9 3737435
} The University of Auckland email : r.sims-at-auckland.ac.nz
} Private Bag 92019
} Auckland
} New Zealand
Ritchie,
Try Jim Nicholino at

JNicolin-at-xraydetectors.com


His regular crystals are terrific and I imagine if he doesn't supply
multilayer crystals, he knows who does.

Ken Converse
owner
Quality Images
Delta, PA



From daemon Tue Oct 31 08:20:12 2000



From: Yurek, Peter :      Peter_Yurek-at-adc.com
Date: Tue, 31 Oct 2000 08:14:31 -0600
Subject: SEM thermal and EBIC imaging references

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello everyone!

I am looking for references (current if possible) for thermal imaging using a thin insulating layer or other thermal imaging techniques that have a minimum of one micron spatial resolution. I am currently working with ridge lasers and would like to characterize the temperatures at the facet and along the ridge. Any help would be appreciated.

I am also looking for a beginner to advanced text on EBIC imaging/contrast mechanisms.

Thanks in advance,

Peter Yurek



From daemon Tue Oct 31 08:29:03 2000



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Tue, 31 Oct 2000 14:19:41 +0000 (GMT Standard Time)
Subject: Re: Emulsion.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Yes, you can process an emulsion by freezing the sample,
fracturing it and viewing it it by cryoSEM (Low Temperature
SEM). The sample is kept frozen on a cold stage.

Dave


On Tue, 31 Oct 2000 08:31:14 +0100 (WAT) "O. O. Ilori"
{sojilori-at-oauife.edu.ng} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Is it possible to view an emulsion under a SEM? By emulsion I mean
} particles suspended in a matrix.
} If possible, how?
}
}
} Mr. O. O. ILORI
} DEPARTMENT OF ELECTRONIC/ELECTRICAL ENGINEERING
} OBAFEMI AWOLOWO UNIVERSITY,
} ILE-IFE, OSUN STATE
} NIGERIA.
}
} email: sojilori-at-oauife.edu.ng
}
}
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"




From daemon Tue Oct 31 09:46:34 2000



From: Paula Sicurello :      patpxs-at-gwumc.edu
Date: Tue, 31 Oct 2000 10:27:05 -0500
Subject: Work with me on this

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi there Boarders!

Long time no hear from me. Did you miss me? I am now on the East Coast in Washington, DC and am looking for someone to be a EM tech. with me. Here is the job announcement:

TEM position available at George Washington University. The Department of Pathology is hiring a TEM technician. Must have experience with embedding, thick and thin sectioning, scoping and darkroom work. Immunochemistry experience a plus. Bachelor's degree and 2 years experience required. Excellent tuition benefits available. If interested please check the GW human resources web site at: www.gwu.edu/cgi-bin/hrs/vacancies
Position title= Electron Microscope Technologist 2
Or contact me at patpxs-at-gwumc.edu or 202-994-2930

This is a clinical laboratory and we work mostly on clinical samples, though we do have some research samples come through (mostly HIV and AIDS related). The lab is pretty low key, but we do have a high standards.

George Washington University is smack dab in the heart of downtown Washington, DC. Walk a few blocks and you're at the White House (my friend even got to pet Buddy the Prez's dog), the mall with all the monuments and all sorts of other cool stuff.

The tuition benefit basically means you can take courses at GW for close to free (pay a reg. fee and buy books). Spouses and kids are eligible for a more limited benefit. Salary commensurate with experience, yada, yada, yada.

So if you're interested, please contact me.


Paula Sicurello
patpxs-at-gwumc.edu
202-994-2930 (phone)
202-994-2518 (fax)




From daemon Tue Oct 31 10:13:55 2000



From: Gudrun.Hugelshofer-at-rdke.nestle.com
Date: Tue, 31 Oct 2000 17:11:33 +0100
Subject: LM - Need help on staining Alginate

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,

I have semi-thin sections of a sample embedded in methacrylate resin
containing alginate and flour among other things. Could anyone give me
advice how to stain the alginate for light microscopy?

Thank your for your help.

Regards, Gudrun

Gudrun Hugelshofer
Nestlé Product Technology Centre Kemptthal, CH-8310 Kemptthal
Tel: ++41 (0)52 354 06 76 Fax: ++41 (0)52 354 07 14
E-mail: gudrun.hugelshofer-at-rdke.nestle.com






From daemon Tue Oct 31 11:48:23 2000



From: Greg Lum :      glum-at-sfsu.edu
Date: Tue, 31 Oct 2000 09:45:10 -0800
Subject: Sorvall Microtome repair

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The servicing/parts for Sorvall microtomes are available from Ventana
Medical Systems who took over RMC. Their address is:

Ventana Medical Systems
RMC-EM
3450 Broadmont, Suite 100
Tucson, AZ 85713
Ph: 520-903-9366
Fax: 520-903-0132

Visit website (www.rmc-scientific.com/microtomes) for parts catalog etc.

I don't have any commercial connection with Ventana, but do have several
Sorvall microtomes.

Greg Lum
EM Facility
San Francisco State University





From daemon Tue Oct 31 12:02:58 2000



From: Bill Miller :      microbill-at-mohawk.net
Date: Tue, 31 Oct 2000 12:59:56 -0500
Subject: Re: Emulsion.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


a word of caution - unless GREAT care is taken in the freezing process
there will be significant production of artifactual structure.... i.e. just
like freezing biological tissue. See any of the many freeze fracture/freeze
etch TEM references.

Bill Miller


At 09:19 AM 10/31/00, Patton, David wrote:
} Microscopy-at-sparc5.microscopy.com




From daemon Tue Oct 31 12:05:21 2000



From: Greg Lum :      glum-at-sfsu.edu
Date: Tue, 31 Oct 2000 10:04:23 -0800
Subject: Sorvall Microtomes again

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I spoke or wrote too soon regarding a source for Sorvall microtome parts.
The following company took over the Sorvall product line from Ventana/RMC:

Boeckeler Instruments, Inc.
4650 South Butterfield Dr.
Tucson, AZ 85714
Ph: 520-745-0001

Again, I have no connection with Ventana or Boeckeler Instruments. I just
want to fix our Sorvall microtomes.

Greg Lum
EM Facility
San Francisco State University
Ph: 415/338-1345
Email: glum-at-sfsu.edu

Greg Lum
Computer/Microscopy Consultant
Electron Microscopy Lab Mgr
Department of Biology
College of Science & Engineering
San Francisco State University
Ph: 415/338-1339
Fax: 415/338-2295
EMail: glum-at-sfsu.edu





From daemon Tue Oct 31 13:29:00 2000



From: Rajesh Patel :      rpatel-at-UMDNJ.EDU
Date: Tue, 31 Oct 2000 14:35:08 -0500
Subject: SEM available

Contents Retrieved from Microscopy Listserver Archives
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We have a functional AMRAY 1400 SEM available. If you have an interest
please
call or email me. Thank you.

Rajesh Patel
Robert Wood Johnson Medical School
Dept. of Pathology
Electron Microscopy Lab
675 Hoes Lane
Piscataway, NJ 08854

(732)235-4648
rpatel-at-umdnj.edu




From daemon Tue Oct 31 15:58:38 2000



From: CBerger338-at-aol.com
Date: Tue, 31 Oct 2000 16:53:48 EST
Subject: Kodak EDAS 290 Electrophoresis

Contents Retrieved from Microscopy Listserver Archives
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We are thinking of purchasing a Kodak EDAS 290 Electrophoresis system. Does
anyone have any experience with this system ( pros & cons), or can recommend
one that they are happy with.

Thanks,

C, Berger



From daemon Tue Oct 31 17:20:17 2000



From: Xinran Liu :      xinran.liu-at-email.swmed.edu
Date: Tue, 31 Oct 2000 17:15:41 -0600
Subject: Help for EM of cultured hippocampal cells

Contents Retrieved from Microscopy Listserver Archives
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We currently doing some EM work on rat cultured hippocampal cells.
The purpose of this experiment is to visualize individual synaptic vesicles
by transmission electron microscopy. The problem we had is that the
resolution/contrast is not high enough to distinguish the synaptic vesicles.

The following is what we did, cells were cultured on coverslips, they were
fixed in situ in 2% glutaraldehyde for 1 hr to 24 hrs, postfixed with 1%
OsO4 for 1 hour, and en bloc stained with 2% aqueous uranyl acetate for 30
min. dehydrated in ethanol and embedded in Polybed812. 40-50 nm sections
were stained by 5% uranyl acetate and lead citrate prior to EM observation.

I would appreciate it very much if someone would provide suggestions on
section staining or cell processing.

Xinran

********************************************
Xinran Liu, M.D., Ph.D.
Center for Basic Neuroscience
The University of Texas Southwestern Medical Center at Dallas
6000 Harry Hines Blvd., NA4.214A
Dallas, TX 75390-9111
Phone: (214) 648-1830
Fax: (214) 648-1801
E-mail: xinran.liu-at-utsouthwestern.edu



From daemon Wed Nov 1 11:45:59 2000



From: Erdem Yasar :      yasar-at-turkuaz.kku.edu.tr
Date: Wed, 1 Nov 2000 15:09:15 +0200 (EET)
Subject: SEM,TEM Available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have a functional JSM 5600 SEM and JEM3010 TEM available. If
you have an interest please call or email me. Thank you.


**********************************
**********************************
** Research.Asst.ERDEM YASAR **
** University of Kirikkale **
** Department of Physics **
** TEM LABORATORY **
** 71450 KIRIKKALE/TURKEY **
** erdem.yasar-at-physics.org **
** yasar-at-science.ankara.edu.tr **
** yasar-at-turkuaz.kku.edu.tr **
**********************************
**********************************









From daemon Wed Nov 1 11:47:06 2000



From: Gordon Couger :      gcouger-at-couger.com
Date: Wed, 1 Nov 2000 04:54:15 -0600
Subject: Re: Ask-A-Microscopist: Frozen sections

Contents Retrieved from Microscopy Listserver Archives
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My amateur results with a microtome have been less than satisfactory.
Embedding in paraffin has been tire some and not very successful.
Sectioning raw or fix material has only worked on a very limited range of
things.

I picked up an old A0 Microtome and a new knife that I believed used
CO2 as a coolant. I can get CO2 but it is a hassle and I have to build
a stage and delivery system for it since the stage is missing.

I am planning on building a simpler stage using copper on an insulating
base and use propane or 1,1,1,2,tetrafloroethane. The tests I have done
with tetrafloroethane look very promising. It reaches -62 f and is very
easy to use. It is normally used to chill parts on circuit boards when
looking for problems. It comes in a spray can with a plastic tube.

Modifying a propane torch is also an easy solution. I have worked with
propane all my life and I am well aware of the dangers. Having to go
outside to use it is one of the main reasons for using the
tetrafloroethane.

My questions are: Will spraying the sample directly with the coolant
cause more damage to the sample than freezing indirectly through the
stage?
If so can I just use a larger sample and trim away the damaged area? Are
there any better readily available coolants?

Thanks
Gordon
Gordon Couger gcouger-at-couger.com
Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00









From daemon Wed Nov 1 12:02:00 2000



From: Dorothy Roak Sorenson :      dsoren-at-umich.edu
Date: Wed, 1 Nov 2000 11:05:24 -0500 (EST)
Subject: Re: Help for EM of cultured hippocampal cells

Contents Retrieved from Microscopy Listserver Archives
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Xinran,

Perhaps the membranes are leaching out during the dehydration and
infiltration. Try a rapid dehydration, such as, two minutes each in 50,
70, 95, and one change of 100% EtOH. Then infiltrate in 3:1 EtOH:Epon for
ten minutes, 1:1 for 10 minutes, 1:3 for twenty minutes, full strength
Epon for thirty minutes, change to fresh full strength Epon and
polymerize. Glutaraldehyde fix for only one hour, maximum, and that is
longer than actually needed for cell monolayers. Stick with your en bloc
staining method prior to dehydration. Also, perhaps thicker sections,
like 70 nm, would increase your contrast.
Good luck!

Dotty Sorenson
Microscopy and Image Analysis Laboratory
Department of Cell and Developmental Biology
University of Michigan Medical School
Ann Arbor, Michigan
(734)763-1170
dsoren-at-umich.edu

On Tue, 31 Oct 2000, Xinran Liu wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} We currently doing some EM work on rat cultured hippocampal cells.
} The purpose of this experiment is to visualize individual synaptic vesicles
} by transmission electron microscopy. The problem we had is that the
} resolution/contrast is not high enough to distinguish the synaptic vesicles.
}
} The following is what we did, cells were cultured on coverslips, they were
} fixed in situ in 2% glutaraldehyde for 1 hr to 24 hrs, postfixed with 1%
} OsO4 for 1 hour, and en bloc stained with 2% aqueous uranyl acetate for 30
} min. dehydrated in ethanol and embedded in Polybed812. 40-50 nm sections
} were stained by 5% uranyl acetate and lead citrate prior to EM observation.
}
} I would appreciate it very much if someone would provide suggestions on
} section staining or cell processing.
}
} Xinran
}
} ********************************************
} Xinran Liu, M.D., Ph.D.
} Center for Basic Neuroscience
} The University of Texas Southwestern Medical Center at Dallas
} 6000 Harry Hines Blvd., NA4.214A
} Dallas, TX 75390-9111
} Phone: (214) 648-1830
} Fax: (214) 648-1801
} E-mail: xinran.liu-at-utsouthwestern.edu
}
}




From daemon Wed Nov 1 12:15:18 2000



From: John Sutliff :      sutliff-at-hkltechnology.com
Date: Wed, 1 Nov 2000 22:25:18 -0500
Subject: Position Available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Xinran Liu wrote:

} We currently doing some EM work on rat cultured hippocampal cells.
} The purpose of this experiment is to visualize individual synaptic vesicles
} by transmission electron microscopy. The problem we had is that the
} resolution/contrast is not high enough to distinguish the synaptic vesicles.
}
} The following is what we did, cells were cultured on coverslips, they were
} fixed in situ in 2% glutaraldehyde for 1 hr to 24 hrs, postfixed with 1%
} OsO4 for 1 hour, and en bloc stained with 2% aqueous uranyl acetate for 30
} min. dehydrated in ethanol and embedded in Polybed812. 40-50 nm sections
} were stained by 5% uranyl acetate and lead citrate prior to EM observation.
}
} I would appreciate it very much if someone would provide suggestions on
} section staining or cell processing.
}
} Xinran
}
} ********************************************
} Xinran Liu, M.D., Ph.D.
} Center for Basic Neuroscience
} The University of Texas Southwestern Medical Center at Dallas
} 6000 Harry Hines Blvd., NA4.214A
} Dallas, TX 75390-9111
} Phone: (214) 648-1830
} Fax: (214) 648-1801
} E-mail: xinran.liu-at-utsouthwestern.edu

Sounds like your sections are too thin for adequate (for your needs)
contrast. Thinner sections are not automatically "better". Try cutting pale gold
sections and see if that helps.
ALSO, check the alignment and stigmation of your microscope. You may need a
different size condernser aperature and/or a different size objective aperature.
All of these can affect contrast and resolution.

Geoff
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************




From root Wed Nov 1 13:57:03 2000
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Dear Cameron,

Thank you very much for your reply (I had a week off and found it
just now) - it was the only reply I have received.

The letter was written and signed by our Senior Technician and
when we sent it, it bounced back, obviously because his name is
not on the list. Therefore we added in the second posting that the
responses should be forwarded to my address - I did not intend to
deprive the microscopy community of the replies and am quite
happy to post this again for all to see - especially your helpful reply.

Thank you very much - if that saves our aging TEM it would make
me very happy.

Regards
Claudia




} From: Cameron Hind {Cameron_Hind-at-baxter.com}


EMPLOYMENT OPPORTUNITY

HKL Technology, a global leader in electron diffraction systems for scanning
electron microscopes, announces an opening in its North American
headquarters in Burnt Hills, New York. Burnt Hills is located in Saratoga
County, in New York’s Capital District, centrally located about 3 hours from
New York City, Boston, and Montreal. HKL Technology is a small, high
technology firm growing in a competitive market. The company is currently
seeking an individual with advanced skills and experience in materials
characterization, which must include scanning electron microscopy and
electron or x-ray diffraction, to take responsibility for our applications
lab. A masters or doctoral degree in physics, materials science, or
geological science is required. Candidates should have positive experience
in teaching others, as training new and existing users is a vital role for
this position. A successful candidate will be gregarious and confident, and
must enjoy traveling. If you are interested in this position, send e-mail
to sutliff-at-hkltechnology.com providing your full name, current address, and
the contact information of two references. Resumes should be sent by
regular mail to the address below.

John A. Sutliff
HKL Technology, Inc.
P.O. Box 179 (801 Saratoga Road)
Burnt Hills, NY 12027
sutliff-at-hkltechnology.com
www.hkltechnology.com
TEL:(518)384-0101
FAX:(518)384-0281




From daemon Thu Nov 2 07:58:56 2000



From: Robert McDonald :      robert-at-starav.geology.gla.ac.uk
Date: Thu, 02 Nov 2000 08:51:35 +0000
Subject: Leo S360 4QBSD

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Anyone know about Semper & Sprynt Boards and can help?
(Send replies to Richard Hunton {Hunton-at-Cardiff.ac.uk} , not
me!)




Hi Folks:

I'm fairly new to this business so I hope that you'll forgive what might
seem to be a 'dumb' question.

I am currently using a LEO / Cambridge S360 SEM with a 4QBSD.
My problem is that when I use probe currents of greater than about 18nA I
get what looks like a reflection of the four diode quadrants of the
detector superimposed on the backscattered image.

This appears (pretty much) independant of the working distance and
magnification. The SE image *does* have a dark spot on the picture where
the centre of the 'reflected' quadrant ghost appears.

I *would* like to be able operate at much higher probe currents than this
if possible. I am using 20 kV and I even changed the filament (you always
hope, don't you?).

Any help would be most gratefully recieved.

Robert


Robert McDonald
SEM & EPMA Laboratories
Department of Geography - Division of Earth Sciences
Gregory Building
Lilybank Gardens
Glasgow University
Glasgow G12 8QQ
Scotland

Tel 0141 330 5505 (SEM lab)
or 0141 330 5442 (Microprobe Lab)
fax 0141 330 4817



From daemon Thu Nov 2 08:15:24 2000



From: Melissa Troost :      m-troost-at-northwestern.edu
Date: Thu, 2 Nov 2000 08:09:54 -0600
Subject: job listing

Contents Retrieved from Microscopy Listserver Archives
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Specimen Preparation Engineer
Northwestern University

The Electron Probe Instrumentation Center (EPIC) at Northwestern University
has an immediate opening for a specimen preparation expert. EPIC is a part
of the world renowned Materials Research Center (MRC) and the Department of
Materials Science & Engineering at Northwestern.
The EPIC facility serves over 120 users in all aspects of Scanning and
Transmission Electron Microscopy. The role of the specimen preparation
engineer is to assist users with their specimen preparation needs, including
instruction in TEM and SEM sample preparation using IBT, FIB, PIPS,
electropolishing, ultramicrotomy, cutting/grinding/polishing, vacuum
evaporation etc.
All microscopes in EPIC are under full service contract. Thus, the duties
include training students/users, development of specialized techniques and
applications, minor maintenance, record keeping and billing.
A BS or technical degree in physical/biological sciences is required. The
candidate must have hands-on experience in all aspects of specimen
preparation as well as considerable familiarity with digital acquisition,
processing and computer assisted techniques. All levels of experience will
be considered. Compensation will be commensurate with experience and
qualifications.
Send cover letter, resume and three references to:
Prof. Vinayak P. Dravid, Director EPIC
Materials Science & Engineering
Northwestern University, 3013A MLSB
Evanston, IL 60208
E-mail: v-dravid-at-northwestern.edu
Fax: (847) 491-7820
http://epic.ms.nwu.edu/epic/index.htm

Northwestern University is an Affirmative Action/Equal Opportunity Employer.
Hiring is contingent upon eligibility to work in the United States.






From daemon Thu Nov 2 08:35:15 2000



From: Debby Sherman :      sherman-at-btny.purdue.edu
Date: 02 Nov 2000 09:31:35 -0500
Subject: Re: Help for EM of cultured hippocampal cells

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------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


It is possible to use an osmium-potassium ferrocyanide fix to enhance membranes (see Karnovsky, 1971, Russell and Burguet, 1977). However, the downside is potential bleaching of ribosomes. I would be interested in hearing from others as to their experience with this type of tissue preparation.
Debby

Debby Sherman Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: sherman-at-btny.purdue.edu
1057 Whistler Building
West Lafayette, IN 47907-1057

On Wednesday, November 1, 2000, Geoff McAuliffe {mcauliff-at-UMDNJ.EDU} wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From daemon Thu Nov 2 09:01:25 2000



From: beth-at-dogwood.botany.uga.edu (Beth Richardson)
Date: Thu, 2 Nov 2000 10:01:12 -0500
Subject: RMC microtome service

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Mime-Version: 1.0
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Hi all,
I need to find someone to work on a RMC 6000-XL ultramicrotome. The machine
has lots of internal vibration on the up/retracted stroke but it is stable
in the cutting stroke...it will cut a section then shake the dookey out of
it so that it is impossible to form a ribbon. The machine is on an
anti-vibration table.
any help would be greatly appreciated.
thanks much,
Beth

**************************************
Beth Richardson
EM Lab Coordinator
Botany Department
University of Georgia
Athens, GA 30602

Phone - (706) 542-1790
FAX - (706) 542-1805
Email - beth-at-dogwood.botany.uga.edu

"Between the two evils,
I always pick the one I never tried before". Mae West (1893-1980)
**************************************





From daemon Thu Nov 2 10:06:30 2000



From: Jo Dee Fish :      jofish-at-burnham-inst.org
Date: Thu, 02 Nov 2000 08:01:22 -0800
Subject: Re: Help for EM of cultured hippocampal cells

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Xinran,
I en bloc stain my cell cultures in 2% UA in 70% EtOH. I leave them in the 4
degree refrigerator overnight, at least 16 hours. The rest of my processing
sounds comparable to yours. This seems to give good contrast. I haven't worked
with hippocampal cell cultures, though, so don't now how it would work on them.
Good luck,
Jo Dee

Xinran Liu wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} We currently doing some EM work on rat cultured hippocampal cells.
} The purpose of this experiment is to visualize individual synaptic vesicles
} by transmission electron microscopy. The problem we had is that the
} resolution/contrast is not high enough to distinguish the synaptic vesicles.
}
} The following is what we did, cells were cultured on coverslips, they were
} fixed in situ in 2% glutaraldehyde for 1 hr to 24 hrs, postfixed with 1%
} OsO4 for 1 hour, and en bloc stained with 2% aqueous uranyl acetate for 30
} min. dehydrated in ethanol and embedded in Polybed812. 40-50 nm sections
} were stained by 5% uranyl acetate and lead citrate prior to EM observation.
}
} I would appreciate it very much if someone would provide suggestions on
} section staining or cell processing.
}
} Xinran
}
} ********************************************
} Xinran Liu, M.D., Ph.D.
} Center for Basic Neuroscience
} The University of Texas Southwestern Medical Center at Dallas
} 6000 Harry Hines Blvd., NA4.214A
} Dallas, TX 75390-9111
} Phone: (214) 648-1830
} Fax: (214) 648-1801
} E-mail: xinran.liu-at-utsouthwestern.edu

--
Jo Dee Fish
Coordinator of Electron Microscopy
Cell Analysis Facility
The Burnham Institute
10901 N. Torrey Pines Rd.
La Jolla, CA 92037
(858)646-3100 ext. 3620





From daemon Thu Nov 2 12:39:14 2000



From: JoAnn Buchanan :      redhair-at-leland.Stanford.EDU
Date: Thu, 02 Nov 2000 10:29:27 -0800
Subject: Re: Help for EM of cultured hippocampal cells

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I routinely prepare hippocampal neurons grown on glass coverslips for
thin section EM using microwave fixation. I fix for 16 seconds (8 on-20
off-8 on) on ice with chilled fixative (2% glutaraldehyde in 0.1M
cacodylate buffer). For secondary fixation, I fix 16 seconds on ice with
chilled 1-2% osmium tetroxide in 0.1M cacodylate buffer containing 0.8%
potassium ferricyanide. I let this sit for 5 mins before proceeding with
30 seconds of 5% en bloc uranyl acetate. Let the dish sit for 30 mins.
Proceed with microwave dehydration (10 seconds each ethanol change, 3 times
100%) and infiltration (5 mins each). I cut 70 nm thin sections (pale
gold/silver) and stain with 5% aqueous UA for 15-30 mins. followed by 3
mins. with lead citrate (made fresh each time).
In regards to Xinran Liu's problem, there could be several factors. The
fixation time is too long- 20 minutes of bench fixation is adequate. Are
you putting sections on formvar? This could reduce contrast. Also, make
thicker sections (60-70nm). Thirdly, check your uranyl acetate. I was
using old UA that had expired and was useless. A new bottle made enormous
difference. Freshly made stains help a lot, particularly for cell cultures
that have low contrast. You should be able to see synaptic vesicles no
problem.
If these things don't help, the problem could be with the health of your
cultures. Synaptic vesicles are the first to go in sick cultures. So make
sure the cultures are healthy and save yourself a lot of time and effort!
Good luck.

JoAnn Buchanan
Molecular and Cellular Physiology
Stanford University School of Medicine
Stanford, CA 94305
650-723-5856



From daemon Thu Nov 2 12:58:34 2000



From: Malis, Tom :      malis-at-nrcan.gc.ca
Date: Thu, 2 Nov 2000 13:50:58 -0500
Subject: RE: RMC microtome service

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dookey? I haven't come across that one yet up here in the Great White
North. Just in case you missed it, RMC announced not too long ago that they
had 're-emerged' from the hiatus caused by Ventana getting out of the EM
products business. They are alive and well in Tucson and could likely
point you in the direction of someone in your area. Contact info:

Al Coritz
Sales & Service Manager
RMC-Boeckeler Instruments
4650 S. Butterfield Dr.
Tucson, AZ 85714
Voice: 520-745-0001
Cell: 520-465-3598
Fax: 520-745-0004
Email:Al-at-Boeckeler.com
Website:RMCProducts.com

Hope you find someone suitable, eh? (That's Canuck-talk).

Tom Malis
Group Leader - Characterization
Materials Technology Laboratory
Natural Resources Canada (Govt. of Canada)
568 Booth St., Ottawa, Canada
ph. 613-992-2310
FAX 613-992-8735
email: malis-at-nrcan.gc.ca



} ----------
} From:
} beth-at-dogwood.botany.uga.edu[SMTP:beth-at-dogwood.botany.uga.edu]
} Sent: Thursday, November 02, 2000 10:01 AM
} To: microscopy-at-sparc5.microscopy.com
} Subject: RMC microtome service
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi all,
} I need to find someone to work on a RMC 6000-XL ultramicrotome. The
} machine
} has lots of internal vibration on the up/retracted stroke but it is stable
} in the cutting stroke...it will cut a section then shake the dookey out of
} it so that it is impossible to form a ribbon. The machine is on an
} anti-vibration table.
} any help would be greatly appreciated.
} thanks much,
} Beth
}
} **************************************
} Beth Richardson
} EM Lab Coordinator
} Botany Department
} University of Georgia
} Athens, GA 30602
}
} Phone - (706) 542-1790
} FAX - (706) 542-1805
} Email - beth-at-dogwood.botany.uga.edu
}
} "Between the two evils,
} I always pick the one I never tried before". Mae West (1893-1980)
} **************************************
}
}
}



From daemon Thu Nov 2 14:01:21 2000



From: Prof. Vinayak Dravid :      v-dravid-at-nwu.edu
Date: Thu, 02 Nov 2000 14:03:13 -0600
Subject: Two TEM Postdoc Positions

Contents Retrieved from Microscopy Listserver Archives
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{html}
{font face="Arial, Helvetica"} POSITION OPEN # 1 of 2 {br}
{br}
Postdoctoral Scholar/Research Associate {br}
{br}
Cryo-Electron Microscopy of Soft and Hybrid Nanostructures {br}
{br}
Northwestern University {br}
Materials Science & Engineering, Evanston, IL {br}
{br}
A postdoctoral scholar/research associate position is immediately
available at Northwestern University in the area of advanced Analytical
Cryo-TEM of soft and hybrid nanostructured materials. {br}
This project is being supervised by Prof. Vinayak P. Dravid, and concerns
with analysis of nanoscale structure, assembly/organization and chemistry
of advanced soft and hybrid nanostructures (such as patterned DNA/Lipids,
nanoparticle-DNA assemblies and nanoparticle-Lipid complexes etc..) using
Analytical TEM and Cryo-EM techniques. This is a part of extensive
cross-disciplinary and collaborative activities in nanotechnology at
Northwestern. As a result, the candidate would have ample opportunity to
learn diverse aspects of nanotechnology, from soft nanolithography to
dynamic measurements, while contributing to Cryo-TEM analysis of soft and
hybrid nanostructures. {br}
Northwestern’s electron probe instrumentation center (EPIC:
{a href="http://epic.ms.nwu.edu/" eudora="autourl"} http://epic.ms.nwu.edu {/a} )
is well equipped with several modern SEMs, TEMs and extensive specimen
preparation equipment ranging from focused ion beam (FIB) to
cryo-transfer with LN2 and LHe stages. One of the cold FEG TEMs (Hitachi
HF-2000) will soon be equipped with a Gatan Imaging Filter (GIF) which
will be extensively utilized in 2-D mapping of element- and bonding
specific spectral signatures in soft and hybrid nanostructures. {br}
The position requires a PhD in physical/biological sciences/engineering.
Considerable hands-on experience in cryo-preparation techniques (e.g.
freeze fracture/drying, ultramicrotomy) for soft/hybrid specimen is
required. Experience in analytical and cryo-TEM techniques and
computation/simulations is necessary. Prior knowledge of imaging
filter/spectral imaging is desirable but not mandatory. {br}
The position is available immediately for at least two years with
possibility for extension for additional years upon mutual agreement.
{br}
Salary and compensation would commensurate with experience, in the range:
$ 30,000-$40,000 per year. {br}
Please forward resume with three names of references to: {br}
Prof. Vinayak P. Dravid {br}
Materials Science & Engineering {br}
Director, Electron Probe Instrumentation Center (EPIC) {br}
2225 N. Campus Drive, 1133 MLSF {br}
Northwestern University, Evanston, IL 60208, USA {br}
Ph.: (847) 467-1363, Fax: (847) 491-7820 {br}
E-mail: v-dravid-at-northwestern.edu {br}
URL:
{/font} {a href="http://vpd.ms.nwu.edu/" eudora="autourl"} {font face="Arial, Helvetica" color="#0000FF"} {u} http://vpd.ms.nwu.edu {br}
{br}
{br}
{/a} {/font} {/u} {font face="Arial, Helvetica"} POSITION OPEN # 2 of 2 {br}
{br}
Postdoctoral Scholar/Research Associate {br}
{br}
Analytical TEM and Electron Holography of Nanostructured Materials {br}
Northwestern University, Evanston, IL, {u} USA {br}
{br}
{/u} A postdoctoral scholar/research associate position is immediately
available at Northwestern University in the area of advanced Analytical
TEM of nanostructured materials. {br}
This project is being supervised by Prof. Vinayak P. Dravid, and concerns
with analysis of nanoscale chemistry, structure and electrostatic fields
at interfaces and junctions in advanced nanostructures (nanocrystals,
nanotubes, thin film interfaces) using analytical TEM and holography
techniques. This is a part of extensive cross-disciplinary and
collaborative activities in nanotechnology at Northwestern. As a result,
the candidate would have ample opportunity to learn diverse aspects of
nanotechnology, from soft nanolithography to dynamic property
measurements while contributing to TEM analysis of nanostructures. {br}
Northwestern’s electron probe instrumentation center (EPIC:
{a href="http://epic.ms.nwu.edu/" eudora="autourl"} http://epic.ms.nwu.edu {/a} )
is well equipped with several modern SEMs, TEMs and extensive specimen
preparation equipment ranging including a focused ion beam (FIB) system.
One of the cold FEG TEMs (Hitachi HF-2000) will soon be equipped with a
Gatan Imaging Filter (GIF) which will be utilized in 2-D mapping of
element- and bonding specific spectral signatures in nanostructures.
{br}
The position requires a PhD in physical sciences/engineering.
Considerable hands-on experience in advanced TEM techniques such as
HRTEM, EDS/EELS, CBED and computation/simulations is required. Experience
in electron holography is desirable but not mandatory. {br}
The position is available immediately for two years with possibility for
extension upon mutual agreement. {br}
Salary and compensation would commensurate with experience, in the range:
$ 30,000-$40,000 per year. {br}
Please forward resume with three names of references to: {br}
Prof. Vinayak P. Dravid {br}
Materials Science & Engineering {br}
Director, Electron Probe Instrumentation Center (EPIC) {br}
2225 N. Campus Drive, 1133 MLSF {br}
Northwestern University, Evanston, IL 60208, USA {br}
Ph.: (847) 467-1363, Fax: (847) 491-7820 {br}
E-mail: v-dravid-at-northwestern.edu {br}
URL:
{/font} {a href="http://vpd.ms.nwu.edu/" eudora="autourl"} {font face="Arial, Helvetica" color="#0000FF"} {u} http://vpd.ms.nwu.edu {br}
{br}
{br}
{br}
{/a} {/font} {/u} {br}
{div} ******************************************************* {/div}
{div} (Vinayak P. Dravid) {/div}
{div} Materials Science & Engineering {/div}
{div} Director, Electron Probe Instrumentation Center (EPIC) {/div}
{div} 2225 N. Campus Drive, 1133 MLSF {/div}
{div} Northwestern University, Evanston, IL 60208, USA {/div}
{div} Ph.: (847) 467-1363, Fax: (847) 491-7820 {/div}
{div} E-mail: v-dravid-at-northwestern.edu {/div}
{br}
{div} {a href="http://nuinfo.nwu.edu/materials/faculty/vpd.html" EUDORA=AUTOURL} http://nuinfo.nwu.edu/materials/faculty/vpd.html {/a}
{/div}
{div} or {/div}
{div} {a href="http://vpd.ms.nwu.edu/" EUDORA=AUTOURL} http://vpd.ms.nwu.edu {/a} {/div}
*******************************************************
{/html}




From daemon Thu Nov 2 14:24:26 2000



From: Bruce Cutler :      bcutler-at-eureka.idl.ukans.edu
Date: 2 Nov 2000 14:19:20 -0600
Subject: SEM Schottky vs. cold FE

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We are in the preliminary throes of writing a grant proposal for a new SEM. This
scope will have the following characteristics: EDX, cathodoluminescence, beam
etching, BS, "conventional" SEM use, possibly variable pressure.
Questions
1. What are the advantages/disadvantages of Schottky over cold FE.
2. I have personally dealt with conventional filament replacement (W), I gather
that FE emitter replacement is much more involved than W filament replacement.
What are practicalities of FE (thermal or cold) emitter replacement with respect
to who performs the work and the cost of the emitter.
3. Is there any reason that variable pressure will preclude the use of the scope
for beam etching.
Thanks to all
Bruce
Bruce Cutler
Director, Microscopy & Electronic Imaging Lab
University of Kansas





From daemon Thu Nov 2 14:30:31 2000



From: Daniel L Flatoff :      dflatoff-at-stu.madison.tec.wi.us
Date: Thu, 02 Nov 2000 20:27:39 GMT
Subject: TEM prep of DLC coating on steel

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Greetings All,
I would like to request guidance on protocols for
preparing TEM cross section to view the interface between
diamond like carbon (DLC) coating on a steel substrate
which has been preimplanted to increase the adhesion of the
coating. Any assistance with be greatly appreciated.



Daniel L. Flatoff





From daemon Thu Nov 2 14:30:31 2000



From: Daniel L Flatoff :      dflatoff-at-stu.madison.tec.wi.us
Date: Thu, 02 Nov 2000 20:27:39 GMT
Subject: TEM prep of DLC coating on steel

Contents Retrieved from Microscopy Listserver Archives
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Greetings All,
I would like to request guidance on protocols for
preparing TEM cross section to view the interface between
diamond like carbon (DLC) coating on a steel substrate
which has been preimplanted to increase the adhesion of the
coating. Any assistance with be greatly appreciated.



Daniel L. Flatoff





From daemon Thu Nov 2 16:15:27 2000



From: The Working Boy :      brmjg-at-TTU.EDU
Date: Thu, 02 Nov 2000 16:09:11 -0600
Subject: Journals

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In the early to mid-90s, our library, due to budget constraints at the
time, let our subscriptions to microscopy journals lapse. We are now in the
position to resubscibe to a number of journals. Could I get advice on which
journals microscopists feel are the most useful. This includes journals in
both the biolgical and physical sciences, applied research electron
microscopy, as well as techniques oriented periodicals. Thanks for your
input.

Mark J. Grimson
Electron Microscopy Facility
Dept. of Biology, MS 3131
Texas Tech University
Lubbock, Texas 79409

(806)742-2704
brmjg-at-ttu.edu





From daemon Thu Nov 2 17:02:13 2000



From: Kellin Defiel :      kellind-at-exploratorium.edu
Date: Thu, 02 Nov 2000 14:49:11 -0800
Subject: Exploratorium Seeks Microscope Interface Designer

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MICROSCOPE INTERFACE DESIGNER -
Public Imaging Facility
Fixed-term Position

The Exploratorium is a museum dedicated to the public understanding of
science, art, and human perception. Founded in 1969 by Frank Oppenheimer,
it has pioneered the role of museums as active teaching centers with
original programming based on an interactive approach to learning. It
serves as an interdisciplinary resource for schools, universities,
scientists, and artists, as well as for the public.

SUMMARY
The Microscope Interface Designer will provide technical expertise to a
three-year project to develop a microscopic imaging facility for the
public, including museum visitors, students, teachers and Internet users,
to view a variety of living specimens via video and the Internet. The
facility will utilize the highest quality optics and state-of-the-art
microscopic techniques and provide a unique experience for the general
public to interact with the technology and tools used by biomedical
researchers. The project will require extensive inter-organizational and
external collaborations with research facilities, equipment and software
companies, teachers and curriculum developers, and media technology
developers. The Microscope Interface Designer will work collaboratively
with the Exploratorium's Life Sciences staff and report to the Life
Sciences Area Manager.

ESSENTIAL FUNCTIONS
· Design and develop approaches for presentation of living specimens to the
public in an accessible and comprehensible manner
· Work collaboratively with the Visitor Research & Evaluation Department to
incorporate visitor feedback in the development process
· Design and develop hardware and software interface systems to enable
public to easily use laboratory grade microscopes
· Work closely with technical experts at equipment and software companies
to develop design and technical options that enhance the project's success
· Design and oversee fabrication of all necessary technical hardware for
visitor interfaces with microscopes
· Work collaboratively with software developers to implement software designs
· Maintain and operate microscopes during the development phase

MINIMUM QUALIFICATIONS
· MS degree in biological sciences with a focus in microscopy, Ph.D. desirable
· Minimum 3 years experience with microscopic imaging techniques
· Demonstrated experience in working with biological specimens of various
sorts, particularly living tissue preparations
· Familiarity with a variety of tissue culturing techniques
· Familiarity with device control and software interfaces for computers
· Proven ability to solve technical design problems
· Ability to work collaboratively as well as independently

HOW TO APPLY: This is a three-year, fixed-term, exempt, union position
which pays $788.63/week. This position starts as soon as possible and ends
on or before September 30, 2003. Please apply to:
Dept. LS-3L
Exploratorium, 3601 Lyon Street, San Francisco, CA 94123
Fax: (415) 561-0370
E-Mail: resume-at-exploratorium.edu (attachments not accepted)
No phone calls, please
The Exploratorium is committed to a diverse workforce

See our web site at www.exploratorium.edu

Kellin Defiel
Human Resources
Exploratorium
San Francisco, CA 94123
kellind-at-exploratorium.edu
(415) 561-0337



From daemon Thu Nov 2 17:06:25 2000



From: Dee Breger :      micro-at-ldeo.columbia.edu
Date: Thu, 2 Nov 2000 17:01:25 -0600
Subject: SEM needs

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Hello colleagues,

Does anyone out there have a 40 kV/LaB6 EHT power supply box (aka Wallace
unit) for a Cambridge 250 Mark 2 that they're willing to donate, sell, or
lend for diagnostics? Or even just the mains inverter board? My users are
stacked in a holding pattern and we'd all be grateful for some help!

Many thanks,
Dee


***************************************************************
Dee Breger
Mgr. SEM/EDX Facility
Lamont-Doherty Earth Observatory
61 Route 9W
Palisades, NY 10964 USA
T: 914/365-8640
F: 914/365-8155

http://www.ldeo.columbia.edu/micro
http://www.discovery.com/area/science/micro/micro1.html
http://www.lsc.org/antarctica/front.html
Journeys in Microspace (Columbia University Press, 1995)





From daemon Thu Nov 2 17:16:44 2000



From: adam.boyes-at-virgin.net ()
Date: Thu, 2 Nov 2000 17:11:05 -0600
Subject: Ask-A-Microscopist: Moving Cells in Microscope

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Colleagues...

Can anyone answer this question . Remember
Ask-A-Microscopist is a forum that is used by NON-MICROSCOPISTS
looking for help so keep try to your technical details at the right level.

Nestor

================================================================
Email: adam.boyes-at-virgin.net
Name: Adam Boyes
School: Horndean Sixth Form

Question: When looking at plant cells containing chloroplasts through a
microscope, why is it that they move around the edges of the cell clockwise
and counterclockwise in adjacent cells?


---------------------------------------------------------------------------





From daemon Thu Nov 2 17:46:23 2000



From: =?utf-8?B?V2FsY2ssIFNjb3R0IEQu?= :      walck-at-ppg.com
Date: Thu, 2 Nov 2000 18:41:12 -0500
Subject: =?utf-8?B?UkU6IFRFTSBwcmVwIG9mIERMQyBjb2F0aW5nIG9uIHN0ZWVs?=

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I assume that you do not have access to a FIB.

What kind of steel? If you have a heat treated hard steel such as 440C or
M50, you are in for a lot of trouble. When you thin these down, the
internal stresses can bend the sample like crazy. You are best off doing a
cross section using the slotted-D approach. If your sample is magnetic, an
added benefit is that it minimizes the amount of magnetic material in the
sample. Slot a rod that fits into a 3mm OD tube. I used 304SS tubing and
rods from Small Parts Catalog. You must thin the sample to where they don't
curl. Cut them down to fit in the slot and epoxy them in. Cut your samples
so that the blade cuts perpendicular to the normal of the layers, i.e. along
the direction of the slot. You should be able to dimple and ion mill
normally. By normally, I mean low angle. The carbon resists ion milling.
You might need to use a gas mixture. We had a little paper in the MRS
Sample Prep IV, Vol 480, that showed a comparison of Ar, Ne, Ne/O2 or Ar/O2
to help with the carbon. Another option is to put water vapor into the ion
mill while you are milling with a leak valve.




-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)



} -----Original Message-----
} From: Daniel L Flatoff [mailto:dflatoff-at-stu.madison.tec.wi.us]
} Sent: Thursday, November 02, 2000 3:28 PM
} To: Microscopy-at-sparc5.microscopy.com; Microscopy-at-sparc5.microscopy.com
} Subject: TEM prep of DLC coating on steel
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
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} ListServer-at-MSA.Microscopy.Com
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}
} --------------------------------------------------------------
} ---------.
}
}
} Greetings All,
} I would like to request guidance on protocols for
} preparing TEM cross section to view the interface between
} diamond like carbon (DLC) coating on a steel substrate
} which has been preimplanted to increase the adhesion of the
} coating. Any assistance with be greatly appreciated.
}
}
}
} Daniel L. Flatoff
}
}
}



From daemon Thu Nov 2 19:02:56 2000



From: E. J. McKenzie :      elizm-at-pdx.edu
Date: Thu, 02 Nov 2000 16:50:21 -0800
Subject: technics ion mill

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Hi everyone,

haven't received a response from my last message so I'll send it again:
does anyone know who supports and supplies parts for the Technics Micro Ion
Mill? (ours was made 1976)

cheers
Liz McKenzie

*******************************************************
Geomicrobiology and Electron Microscopy Laboratory
Room S9 Cramer Hall
1721 SW Broadway
Portland State University
Portland
OR97201

ph:503 725 3362
fax:503 725 3025

*******************************************************

It is possible that your car engine is driven by psycho-kinetic energy.
But if it looks like a petrol engine and smells like a petrol engine, a
sensible working hypothesis is that it is a petrol engine.

*******************************************************




From daemon Thu Nov 2 21:49:16 2000



From: EBMet-at-aol.com
Date: Thu, 2 Nov 2000 22:43:33 EST
Subject: Preparation of solder joint cross-sections

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I would appreciate any procedures or references concerning preparation of
solder joints for microelectronic applications. The solders that I am
concerned with are high Pb, Pb-Sn-Ag and Au-Sn. Thanks.

Elliot Brown



From daemon Fri Nov 3 08:39:21 2000



From: Anthony Garratt-Reed :      tonygr-at-mit.edu
Date: Fri, 03 Nov 2000 09:33:11 -0500
Subject: Re: SEM Schottky vs. cold FE

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The advantages/disadvantages of cold vs. Schottky FE guns lie at the
extremes of performance. Cold FE will give you better ultimate image
resolution (roughly 1.5 vs. 2.5 nm, depending on manufacturer, of course)
and will give you noticably better images at low voltage (1kV or so).
Schottky will give you much higher currents at larger spot sizes (for x-ray
mapping or back-scatter diffraction pattern acquisition, for example) and
much better medium-term (tens of minutes to a few hours) beam current
stability, if that is important to you. It is also not necessary to flash
the tip periodically in a Schottky gun. For most other SEM needs, either
form will work wonderfully for you.

We have one of each, and we do not notice any difference in the maintenance
needs between them. Don't worry about tip replacements. Our cold FE is
nearly 6 years old and is still running with the original tip, as is our
Schottky, which is over three years old. I don't think this is a function
of vendor - it is my impression that microscopes from other vendors have
similar records. The tip replacement is performed by the service
engineers, and depending on your service contract, may be included in that.
It takes about 3 days (I am told!) on either instrument.

It is much more important to be clear about your microscopy needs. You say
in your posting "possibly variable pressure" ... there is a big difference
between the ESEM of FEI/Philips and the variable pressure designs of other
manufacturers, so you need to be clear about how important this factor is
to you. What structures will you need to examine by "conventional" SEM,
how important is high-speed x-ray mapping, etc. etc. etc. By asking these
kinds of questions the right microscope will eventually become clear to
you. Don't worry about the mechanics of the gun - they all work fine.

All in my humble opinion, of course!!

Tony Garratt-Reed.



At 02:19 PM 11/02/2000 -0600, you wrote:
} ------------------------------------------------------------------------
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** Anthony J. Garratt-Reed
** MIT Room # 13-1027
** 77 Massachusetts Avenue
** Cambridge, MA 02139-4307
** USA
**
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** Fax: (+) 1-617-258-6478
**





From daemon Fri Nov 3 08:42:41 2000



From: Norm Olson :      nho-at-bilbo.bio.purdue.edu
Date: Fri, 3 Nov 00 09:39:19 -0500
Subject: Fwd: Re: Freeze-plunge device availability

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{Subject: Re: Freeze-plunge device availability
{From: Dennis C. Winkler, DCWinkler-at-aol.com

{Last month you posted a query about the availability of freeze-plunge
devices.

{I am interested in the responses you received. I didn't find them
{posted on the listserver or on your web site. Would you mind sharing
{them?

I have not had a chance to post them yet but here is the information. I
received
one response and I knew of one other vendor.

1) BAL-TEC Plunge Freezing System TFD 010
Contact Johnny Hagen at 603-622-5011 for the details.
They have a single picture on their website www.bal-tec.com
List price for the system is $8674

2) This summer at MSA I saw that Gatan had a system.
Our sales person is Paul Miller, 724-779-2501
Their website is www.gatan.com but I saw no information on this system
there the last time I looked.
List price ~$20,000 (ball park figure)

The two systems do quite different things. The Bal-tec system is
designed to keep the cryogens at the proper temperature and it has a
pneumatic cylinder plunging arm. The Gatan system has an enclosed
chamber around the sample to maintain humidity and you program in how
long you want to do wicking before you plunge. You can do single sided
or double sided wicking and it looks like it has a fair amount of
flexibility for doing different types of plunge experiments.

As usual... the disclaimer is that I am not receiving kickbacks from
either company on this.

Norm Olson

*******************************************
Norm Olson
Senior Research Electron Microscopist
Department of Biological Sciences
Lilly Hall of Life Sciences
Purdue University
West Lafayette, IN 47907

Phone: 765-494-5643
FAX: 765-496-1189
email: nho-at-bragg.bio.purdue.edu
http://bilbo.bio.purdue.edu/~nho/index.htm
http://bilbo.bio.purdue.edu/~baker/

*******************************************




From daemon Fri Nov 3 09:11:11 2000



From: anderron-at-us.ibm.com
Date: Fri, 3 Nov 2000 10:07:06 -0500
Subject: Re: Preparation of solder joint cross-sections

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Importance: Normal



We tripod polish with some modification to the procedure:
You can't use water to polish the specimen, we use propylene glycol from a
squeeze bottle when actually polishing the solder;
The thin solder section is mechanically weak, we mount on a Mo grid with a
small aperture size prior to second side polishing;
Use low-angle ion milling to keep the solder from melting and to minimize
element-selective milling in the ion mill if you have to ion mill the
specimen.
Use a cold stage in the TEM or restrict the beam heating.

Good luck,

Ron



Ron Anderson, IBM, Hopewell Jct., New York, USA. anderron-at-us.ibm.com

IBM Analytical Services; http://www.chips.ibm.com/services/asg




From daemon Fri Nov 3 10:28:59 2000



From: Carrie Golash :      cdg126-at-psu.edu
Date: Sat, 04 Nov 2000 11:22:45 -0500
Subject: Fwd: Troubleshooting oocyte sectioning

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}
} Hello all -
}
} I've written to the list in the past regarding embedding of bovine
} oocytes and I wish to express thanks to all of the helpful suggestions that
} I received.
}
} I'm still having a coupld of problems, though, so I'm again seeking
} advice. I am fixing oocytes in gluteraldehyde and then embedding them in
} agarose. I am using 1% low melting point agarose, creating a bubble into
} which I place my oocytes and then sealing the bubble with 0.5% agarose. I
} then trim the agarose into a 1 mm^2 chip and dehydrate through a series of
} ethanol steps, including staining with eosin to help visualize the eggs.
} Finally, I clear in xylenes and embed the chip in a paraffin block.
}
} My problem is twofold: 1) following sectioning and rehydration,
} when I
} look at the agarose sections, I can certainly see oocytes (they stain
} specifically for an anti-ZP antibody)and they're consistent in placement
} among consequent sections, but frankly they're horribly ugly. The zona is
} "wrinkled", and the eggs look like they've undergone an osmotic trauma. 2)
} The agarose sections seem to frequently fold or rip and I'm not quite sure
} at what stage of the process that this is occuring - I feel when placing
} the sections into the waterbath or retrieving them on slides (treated with
} HistoPrep) they seem fine. Is it possible the folding is occuring during
} the deparaffination/rehydration, maybe because the agarose sections don't
} stick well to the slides?
}
} Any help in this matter is greatly appreciated! Thanks!
}
} --Carrie

Carrie Golash
John O. Almquist Research Center
Penn State University
University Park, PA 16802
W: (814) 865-5896
H: (814) 692-7926
http://www.das.psu.edu/dbrc/dbrc.htm



From daemon Fri Nov 3 10:40:15 2000



From: Gordon Vrololjak :      gvrdolja-at-nature.Berkeley.EDU
Date: Fri, 3 Nov 2000 08:36:46 -0800 (PST)
Subject: instruction manuals

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,
Anyone know if they have manuals for a FC4D Reichert cryounit
attachment for a Cryo ultramicrotome? Please contact me personally if
so.

Gordon.

\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
Gordon Ante Vrdoljak Electron Microscope Lab
ICQ 23243541 http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
gvrdolja-at-nature.berkeley.edu UC Berkeley
phone (510) 642-2085 Berkeley CA 94720-3330
fax (510) 643-6207 cell (510) 290-6793





From daemon Fri Nov 3 10:48:27 2000



From: simon watkins :      swatkins+-at-pitt.edu
Date: Fri, 03 Nov 2000 11:35:23 -0500
Subject: automated immunohistochemistry rigs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Folks, heres a new thread.... We are curious about how well automated
ICC/IHC machines work
How easy are they to use?
How flexible are the programs?
How much time do they save?
Can you program sophisticated multicolor protocols?
(of course the inflammatory question) which is the best machine?
Whats the reagent usage like?
etc

Looking forward to hearing from you all
Simon


---------------------------
Simon C. Watkins Ph.D. MRC Path
Associate Professor, Cell Biology and Physiology
Director: Center for Biologic Imaging
BSTS 225
University of Pittsburgh
Pittsburgh PA 15261
Tel:412-648-3051
Fax:412-648-8330
http://sbic6.sbic.pitt.edu
-----------------------------------




From daemon Fri Nov 3 12:07:45 2000



From: NPGSlithography-at-aol.com
Date: Fri, 3 Nov 2000 13:01:58 EST
Subject: Re: SEM Schottky vs. cold FE

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Bruce,

In the past, cold FE sources gave the best resolution, however, Schottky
(thermal) FE sources have improved significantly over the years. Since the
"resolution" specifications provided by the SEM vendors cannot always be
compared directly, it is best to use your own samples and observe the imaging
capabilities of each SEM for yourself. Such demos are common before the sale
of FE SEMs.

A difference between cold FE and Schottky FE sources that is not subjective
is the stability of the beam current. Cold FE sources will typically have
frequent spikes and/or jumps of the beam current on the order of +/-3% or
more. Readings of the beam current taken just seconds apart may show changes
on this order, and over 1 hour intervals changes of +/-10% or more are not
unusual. In contrast, the FE sources are exceptionally stable with total
noise and drift of less than 1%/hour. (I've measured ~1% drift over 12 hours
in one case.)

If you are concerned about beam current noise and drift, it may be helpful to
request a graph of beam current vs. time showing the typical performance of
the SEM in question. (But be wary of chart recording traces that may have a
slow time constant which will hide fast changes in the beam current!)

Note that for imaging, such noise/drift in the beam is not generally an
issue, because the cold FE SEMs dynamically adjust the brightness displayed
on the imaging screen to compensate for the changes in the actual beam
current. In effect, the beam intensity on the specimen is still changing,
but the displayed image does not show it.

If you need high beam currents, the Schottky source will typically give much
more current than a cold FE source.

Regarding replacement of the FE source, that is generally done under the SEM
service contract. The price I have heard for the FE source itself is ~$20k
US, although I'm sure others can give more exact numbers. The SEM vendors
themselves will be the best source of information regarding pricing.

Variable pressure SEMs will typically have a "high vacuum" mode for
conventional viewing. However, a variable pressure SEM may have an extra
aperture to support the differential pumping in the column that may limit the
lowest magnification, even in non-VP mode.

I hope this helps.

Joe
_________________________________________
Joe Nabity, Ph.D.
JC Nabity Lithography Systems
E-Beam Lithography using Commercial SEMs & STEMs
PO Box 5354, Bozeman, MT 59717 USA
Voice: (406) 587-0848
FAX: (406) 586-9514
E-mail: info-at-jcnabity.com
Web: www.jcnabity.com

In a message dated 11/2/2000 7:04:37 PM Mountain Standard Time,
bcutler-at-eureka.idl.ukans.edu writes:

} We are in the preliminary throes of writing a grant proposal for a new SEM.
} This
} scope will have the following characteristics: EDX, cathodoluminescence,
} beam
} etching, BS, "conventional" SEM use, possibly variable pressure.
} Questions
} 1. What are the advantages/disadvantages of Schottky over cold FE.
} 2. I have personally dealt with conventional filament replacement (W), I
} gather
} that FE emitter replacement is much more involved than W filament
} replacement.
} What are practicalities of FE (thermal or cold) emitter replacement with
} respect
} to who performs the work and the cost of the emitter.
} 3. Is there any reason that variable pressure will preclude the use of the
} scope
} for beam etching.
} Thanks to all
} Bruce
} Bruce Cutler
} Director, Microscopy & Electronic Imaging Lab
} University of Kansas
}





From daemon Sat Nov 4 05:14:22 2000



From: richard black :      m02jmy00-at-cwcom.net
Date: Sat, 04 Nov 2000 10:52:03 +0000
Subject: Preparation of solder joint cross-sections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I was involved in preparation, and microscopy, of lead solders for some
time at my last job, and if you wish to contact me I will be able to put
you in touch with the group that I was working with.




From daemon Sun Nov 5 07:58:33 2000



From: Mendes, Maria :      mmendes-at-mtsinai.on.ca
Date: Sun, 5 Nov 2000 07:33:31 -0600
Subject: SPURR will be discontinued?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello I would like to subscribe to your service, please let me know if there
is any service charge.
Please pass on my problem to your subscribers.
I am a research lab that does a lot of hard tissue processing, I as well as
our EM departments have been using SPURR as our plastic of choice. I have
been recently told by my supplier (Marivac) that in six months one of the
components of SPURR will be discontinued. The component is ERL
(Vinylcyclohexene Dioxide). I would like to know if others are using SPURR
and if so have they encountered the same problem. Please forward your
information to mmendes-at-mtsinai.on.ca.
Thank-you in advance for any help offered.

Maria





From daemon Sun Nov 5 10:10:55 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Sun, 5 Nov 2000 09:57:26 -0600
Subject: Administrivia: Listserver Archives Updated

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Colleagues...

The on-line Monthly Listserver Archives and Search Engine have been updated
and are now current through Oct 2000

http://www.msa.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Cheers
Nestor
Your Friendly Neighborhood SysOp




From daemon Mon Nov 6 01:21:17 2000



From: Tim Akinbo :      takinbo-at-onebox.com
Date: Mon, 06 Nov 2000 08:11:44 +0100
Subject: Re: Low Frequency Surface Microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greetings.
I am a final student of the department of Electrical Electronics,
Obafemi Awolowo University, Ile-Ife, Nigeria.
I am doing a project titled "Low-Frequency Surface Microscopy". My
supervisor wants me to use an electric field-probe to detect changes in
the capacitance of the surface as cracks, boundaries, etc. are
encountered. I would be very grateful if you can send me any
information on the topic.

My e-mail address is: femi_adeluyi-at-yahoo.com and my address is:
Olufemi Adeluyi
Department of Electronic and Electrical Engineering
Obafemi Awolowo University,
Ile-Ife, Nigeria.

Thanking you in anticipation.




From daemon Mon Nov 6 01:52:27 2000



From: erich-at-ento.csiro.au (Eric Hines)
Date: Mon, 6 Nov 2000 18:46:04 +1100
Subject: SEM, forensic techniques

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear all,
We have been asked to visualize the manufacturers stamped numbers on a
rifle which have been removed by grinding. So far secondary and backscatter
images don't tell us much.
We are told that the metal under the stamping is compressed for a
significant depth. Any ideas on how to image this compressed metal.
Cheers,
Eric Hines
Microscopy Centre
CSIRO Entomology
Canberra OZ





From daemon Mon Nov 6 04:18:29 2000



From: Krzysztof Jan Huebner :      hubner-at-IOd.krakow.pl
Date: Mon, 6 Nov 2000 11:05:00 +0100 (MET)
Subject: Re: SEM, forensic techniques

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Hi,

you can use the macro etching techniques and macro photography;
more details in the book
Vander Voort - Metallography, Principles and practise, McGraw-Hill

best regards

KJ Huebner

{hubner-at-IOd.krakow.pl} :-)

On Mon, 6 Nov 2000, Eric Hines wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear all,
} We have been asked to visualize the manufacturers stamped numbers on a
} rifle which have been removed by grinding. So far secondary and backscatter
} images don't tell us much.
} We are told that the metal under the stamping is compressed for a
} significant depth. Any ideas on how to image this compressed metal.
} Cheers,
} Eric Hines
} Microscopy Centre
} CSIRO Entomology
} Canberra OZ
}
}
}
}



From daemon Mon Nov 6 07:20:12 2000



From: Platek, Frank :      FPLATEK-at-ora.fda.gov
Date: Mon, 6 Nov 2000 06:26:27 -0500
Subject: SEM Visualization of Restored Serial Numbers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Eric,

As promised, the only reference for SEM visualization of damaged serial
numbers in gun metal I was able to quickly find was "Visualization of a
Restored Serial Number Using Scanning Electron Microscopy (SEM)" by Amy
Mongan of Forensic Analytical Specialties. The article was published as a
case report in the Journal of Forensic Sciences [J Forensic Sci
1996;41(6):1074-1076].
There are more than likely other references but I do not have them
immediately available.

Good luck!

S. Frank Platek, M.S.
Forensic Chemistry Center
U.S. Food and Drug Administration
6751 Steger Drive
Cincinnati, OH 45237-3097
(513) 679-2700 X254
(513) 679-2761 FAX
fplatek-at-ora.fda.gov

Disclaimer: Opinions/recommendations stated are solely mine and do not
necessarily represent those of the US Food and Drug Administration or any
other Federal Agency.





From daemon Mon Nov 6 07:24:20 2000



From: Co2clean-at-aol.com
Date: Mon, 6 Nov 2000 08:20:18 EST
Subject: Re: SEM, forensic techniques

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


There is a special etch that "highlights" the stampings, forget SEM methods.
I do not recall the exact mix, but Police forensics lab know it. I believe
it is a mild form of aqua reiga with one added item but forgot. I consulted
to a forensics lab and did it once and we got a few digits off an engine
block.

Good luck

Robert Sherman
Applied Surface Technologies
www.co2clean.com


}
} Dear all,
} We have been asked to visualize the manufacturers stamped numbers on a
} rifle which have been removed by grinding. So far secondary and backscatter
} images don't tell us much.
} We are told that the metal under the stamping is compressed for a
} significant depth. Any ideas on how to image this compressed metal.
} Cheers,
} Eric Hines
} Microscopy Centre
} CSIRO Entomology
} Canberra OZ



From daemon Mon Nov 6 07:47:39 2000



From: Purdy, Sam :      SPurdy-at-nationalsteel.com
Date: Mon, 6 Nov 2000 07:42:02 -0600
Subject: Eric Hiness/ rifle barrel

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Eric:

Some forensic scientists have been successful in detecting stamped
serial numbers by etching the part in a solution of 5 -10% HNO3 in water or
alcohol for 10 to20 seconds. The disturbed metal will etch more darkly than
the surrounding metal.

Best regards and good luck,

Sam Purdy
Tecnical Center
National Steel Corp.
Trenton MI
spurdy-at-nationalsteel.com





From daemon Mon Nov 6 08:21:39 2000



From: Pam Marcum :      pmarcum-at-polysciences.com
Date: Mon, 6 Nov 2000 09:13:39 -0500
Subject: automated immunohistochemistry rigs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The immuno units available are becoming many and varied. However, the main
consideration is flexibility of the unit and secondarily cost of the
reagents. Dako and Cytlologix are the most reliable and flexible available
for routine and special procedures. I believe both can be programmed for
dual staining or to separate protocols can be used on tissue for the result.
These units allow you to choose your reagents, antibodies and procedures.
The Ventana is an excellent unit with one huge drawback you are forced to
use their secondaries and limited on primaries to some extent. The cost is
very high for the reagents to run the unit. I am a histologist who also
does EM and can speak from experience of working with the units in the field
as an observer and user. Pam Marcum

-----Original Message-----
} From: simon watkins [mailto:swatkins+-at-pitt.edu]
Sent: Friday, November 03, 2000 11:35 AM
To: microscopy


Folks, heres a new thread.... We are curious about how well automated
ICC/IHC machines work
How easy are they to use?
How flexible are the programs?
How much time do they save?
Can you program sophisticated multicolor protocols?
(of course the inflammatory question) which is the best machine?
Whats the reagent usage like?
etc

Looking forward to hearing from you all
Simon


---------------------------
Simon C. Watkins Ph.D. MRC Path
Associate Professor, Cell Biology and Physiology
Director: Center for Biologic Imaging
BSTS 225
University of Pittsburgh
Pittsburgh PA 15261
Tel:412-648-3051
Fax:412-648-8330
http://sbic6.sbic.pitt.edu
-----------------------------------





From daemon Mon Nov 6 08:48:08 2000



From: Pam Marcum :      pmarcum-at-polysciences.com
Date: Mon, 6 Nov 2000 09:44:02 -0500
Subject: SPURR will be discontinued?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Maria,
We are not discontinuing Spurr's at Polysciences. We do have ERL and the
other components available now and in the future.

Pamela A Marcum
Histology/Microscopy
Product Development Manager
Polysciences, Inc.
400 Valley Road
Warrington, PA 19876
Phone: 800-523-2575  Ext 167
Fax: 215-343-0214
E-Mail: pmarcum-at-polysciences.com {mailto:pmarcum-at-polysciences.com}


-----Original Message-----
} From: Mendes, Maria [mailto:mmendes-at-mtsinai.on.ca]
Sent: Sunday, November 05, 2000 8:34 AM
To: Microscopy-at-sparc5.microscopy.com


Hello I would like to subscribe to your service, please let me know if there
is any service charge.
Please pass on my problem to your subscribers.
I am a research lab that does a lot of hard tissue processing, I as well as
our EM departments have been using SPURR as our plastic of choice. I have
been recently told by my supplier (Marivac) that in six months one of the
components of SPURR will be discontinued. The component is ERL
(Vinylcyclohexene Dioxide). I would like to know if others are using SPURR
and if so have they encountered the same problem. Please forward your
information to mmendes-at-mtsinai.on.ca.
Thank-you in advance for any help offered.

Maria






From daemon Mon Nov 6 11:14:00 2000



From: William H Roberts :      William.H.Roberts-at-usa.dupont.com
Date: Mon, 6 Nov 2000 11:47:14 -0500
Subject: SEM, forensic techniques

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Eric,

It has been many years since I worked in a Crime Lab, but the technique
that we used to raise serial numbers on guns did not involve the SEM at
all. First, we polished the surface where the serial number had been
ground off using successively finer grades of sandpaper. When we had
achieved a highly polished appearance to the surface we then brushed the
area with a dilute solution of nitric acid using a cotton tipped swab.
This was done very carefully, inspecting the surface after each wipe with
the swab using a collimated light beam held at a shallow angle with respect
to the surface. The numbers will appear due to the fact, as you mentioned,
they are compressed more than the surrounding metal and therefore are
etched at a different rate. When the numbers are visible, the surface is
flushed with water and a non-volatile liquid like glycerine is applied to
keep the surface wet until the numbers can be recorded and photographed. A
hand lens or a stereomicroscope were the only microscopical tools
necessary. I know that this description is sketchy, but it should give you
a starting point. Also, keep in mind that if the person who was trying to
remove the serial number knew his business, he (or she) may have ground
deeply enough to obliterate any trace of the number and no method will
restore it.

Regards, Bill Roberts




erich-at-ento.csiro.au (Eric Hines) on 11/06/2000 02:46:04 AM

To: Microscopy-at-sparc5.microscopy.com
cc: (bcc: William H Roberts/US/FILM/DPT)

Dear all,
We have been asked to visualize the manufacturers stamped numbers on a
rifle which have been removed by grinding. So far secondary and backscatter
images don't tell us much.
We are told that the metal under the stamping is compressed for a
significant depth. Any ideas on how to image this compressed metal.
Cheers,
Eric Hines
Microscopy Centre
CSIRO Entomology
Canberra OZ









From daemon Mon Nov 6 11:16:57 2000



From: werner-at-rosharon.wireline.slb.com (Andrew Werner)
Date: Mon, 06 Nov 2000 11:14:13 -0600
Subject: Re: SEM, forensic techniques

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Eric,

Light optical metallographic techniques, rather than electron optical,
might serve you in this case.

If you polish the region - removing as little material as possible, but
through 6 micron diamond anyway - and etch with 2% nital (2% nitric acid in
alcohol) - you ought to reveal the underlying numbers. Other people may
suggest more effective etchants for this purpose; nital is just a general
purpose old standby.

Regards,
Andrew T. Werner
Shaped Charge Research - Metallurgy Laboratory
Schlumberger Reservoir Completions Technology Center
14910 Airline Road, Rosharon, TX 77583-1590
Voice (281) 285-5272 Fax (281) 285-5273

"We shoot the hippopotamus
with bullets made of platinum
'cause if we used the leaden ones
his hide would surely flatten 'em"
- Author Unknown

At 06:46 PM 11/06/2000 +1100, Eric Hines wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From daemon Mon Nov 6 12:30:46 2000



From: michael shaffer :      epmalab-at-darkwing.uoregon.edu
Date: Mon, 6 Nov 2000 10:27:02 -0800
Subject: SEM: cathodo-luminescence references

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



One of my users has just asked me for references regarding the
technique of acquiring electron induced color CL images by obtaining
RGB channels via red-blue-green filters. One of those procedures we
have simply been taking for granted, but now want to acknowledge
earlier work, especially with respect to geologic materials and
quartz.

cheerios, =shAf= :o)

{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {}
Michael Shaffer, R.A. - mshaf-at-darkwing.uoregon.edu
Geological Science's Electron Probe Facility - University of Oregon
http://epmalab.uoregon.edu/





From daemon Mon Nov 6 13:47:25 2000



From: Chris Baker :      chris-at-collegewafer.com
Date: Mon, 06 Nov 2000 14:43:30 -0800
Subject: Ultrathin Si, GaAs, SOI, InP, Ge, GaN, 12" Si & Glass

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Visit http://www.collegewafer.com for your free quote, or call (800) 713-9375
or Fax (888)-832-0340!!! E-mail us at chris-at-collegewafer.com

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Other Wafers IN STOCK & READY to SHIP Si, GaAs, Ge, InP, InAs, InSb, GaSb, GaN,
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Also visit http://www.thecoveredcall.com learn to pick winning stocks using he
CANSLIM method of investing with a covered call twist!

ssf




From daemon Mon Nov 6 13:58:57 2000



From: Gordon Couger :      gcouger-at-couger.com
Date: Mon, 6 Nov 2000 13:58:20 -0600
Subject: Re: Low Frequency Surface Microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



If you can submerge the specimen under a liquid it will greatly enhance
the captive effects from 3 or so for most fluids to 70 for water due to
the dielectric properties of the liquid.

I have no idea if this is possible but if it is you can get a great deal
of improvement in resolution in differences in height. Cracks in
particular would be highlighted.

Gordon
Gordon Couger gcouger-at-couger.com
Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00
} From: "Tim Akinbo" {takinbo-at-onebox.com}
}
} Greetings.
} I am a final student of the department of Electrical Electronics,
} Obafemi Awolowo University, Ile-Ife, Nigeria.
} I am doing a project titled "Low-Frequency Surface Microscopy". My
} supervisor wants me to use an electric field-probe to detect changes in
} the capacitance of the surface as cracks, boundaries, etc. are
} encountered. I would be very grateful if you can send me any
} information on the topic.
}
} My e-mail address is: femi_adeluyi-at-yahoo.com and my address is:
} Olufemi Adeluyi
} Department of Electronic and Electrical Engineering
} Obafemi Awolowo University,
} Ile-Ife, Nigeria.
}
} Thanking you in anticipation.
}
}





From daemon Mon Nov 6 16:08:50 2000



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Mon, 06 Nov 2000 17:02:15 -0500
Subject: ERL 4221

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Maria Mendes wrote:
==========================================
I am a research lab that does a lot of hard tissue processing, I as well
as our EM departments have been using SPURR as our plastic of choice. I
have
been recently told by my supplier (Marivac) that in six months one of the
components of SPURR will be discontinued. The component is ERL
(Vinylcyclohexene Dioxide). I would like to know if others are using SPURR
and if so have they encountered the same problem. Please forward your
information to mmendes-at-mtsinai.on.ca.
=========================================
This posting generated some worried communcations with customers asking
about the continued availability of ERL 4221 (Vinylcyclohexene Dioxide).

The manufacturer reports to us no plans to discontinue ERL 4221 and there
seems to be enough in the distribution pipelines to last some years into the
future. ERL 4221 is currently available from SPI Supplies as I suspect it
is available also from the other major suppliers of chemicals to the
microscopy world.

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.2spi.com
########################
============================================






From daemon Mon Nov 6 16:22:37 2000



From: James Roberts :      James.Roberts-at-mail.co.ventura.ca.us
Date: Mon, 06 Nov 2000 14:17:24 -0800
Subject: Re: Eric Hiness/ rifle barrel

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Depending upon the steel the receiver is made from a Fry's or Modified Fry's reagent (a cupric Chloride in HCl solution) works much better than the HNO3 but both alternated works still better. As I said in a previous post on this the NASA publication has quite a good rundown on all of the appropriate solutions. Hatcher, Jury and Weller a less detailed but acceptable section on it. The details of how to prepare the metal is as important as the solutions chosen if it is to work. Drop me a note and I'll write the procedure up if you can't find the NASA publication.

Jim Roberts

James L. Roberts
Firearm and Toolmark Examiner
Ventura Co. Sheriff's Lab
(805) 654-2308
James.Roberts-at-mail.co.ventura.ca.us

} } } "Purdy, Sam" {SPurdy-at-nationalsteel.com} 11/06/00 05:42AM } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Eric:

Some forensic scientists have been successful in detecting stamped
serial numbers by etching the part in a solution of 5 -10% HNO3 in water or
alcohol for 10 to20 seconds. The disturbed metal will etch more darkly than
the surrounding metal.

Best regards and good luck,

Sam Purdy
Tecnical Center
National Steel Corp.
Trenton MI
spurdy-at-nationalsteel.com









From daemon Mon Nov 6 16:39:13 2000



From: Sara Miller :      saram-at-duke.edu
Date: Mon, 6 Nov 2000 17:33:28 -0500 (EST)
Subject: Confocal Technical Position Open (fwd)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Experienced Confocal Microscopy/Electron Microscopy Technologist to work
in Research Laboratory of Department of Pathology


The person we seek will be responsible for organization of a new facility
that includes a confocal microscope and an electron microscope. The
position includes overall management of the microscopy facility, user
training, and user supervision. Requirements for the position include
experience with light and transmission electron microscopy. This
individual will oversee all aspects of specimen accession and processing,
operation of the microscopes, photography, record keeping, and
supervision of a technician. Knowledge of EM, biology, and pathology, as
well as photographic procedures is required. The individual will work
with only minimum supervision.

Position Requirements

Experience with confocal and digital imaging techniques, microinjection,
visualization of living cells containing fluorescent probes,
photobleaching, and fluorescence in situ hybridization.

Minimum of five years experience working in an electron microscopy
laboratory with hands-on experience in tissue processing, dark room
photography, and operation and maintenance of electron microscope.

Two years experience working on electron microscopy of human tissue
specimens with knowledge of histology and pathology.

Excellent interpersonal and organizational skills are essential.

BachelorUs degree in science desirable.

Desirable Experience

Expertise in training in the operation of confocal microscope systems is
a distinct advantage.

Familiarity with light microscopy methods, immunofluorescent staining,
use of fluorescent probes for physiologic measurements and the general
principles of cell biological research are critical. Significant facility
with computers is desired.

Responsibilities

Serve as the technical manager of the facility and be responsible for the
operation and maintenance of the confocal and EM microscope facility.

Perform routine transmission EM, including tissue processing,
ultramicrotomy, and examination; do preventative maintenance on the
equipment; maintain the lab, order supplies, schedule instruments, and
oversee billing.

Image analysis at the light, confocal and electron microscopic levels and
preparation of micrographs for publication.


Send resume to:
Suzanne Mirra, M.D., Chair
Department of Pathology
Box 25
SUNY-Brooklyn
450 Clarkson Av.
Brooklyn, NY 11203-2098

-----------------
Submitted by
Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265





From daemon Mon Nov 6 16:43:05 2000



From: Sara Miller :      saram-at-duke.edu
Date: Mon, 6 Nov 2000 17:35:14 -0500 (EST)
Subject: Histotechnologist Position Open (fwd)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Experienced Histotechnologist to work in Neuropathology Research
Laboratory of Department of Pathology

The person we seek will be responsible for maintaining a neuropathology
research laboratory in the Department of Pathology at SUNY Downstate
Medical Center. Knowledge of immunohistochemistry, special stains, and
histopathology is required. Previous experience in handling central
nervous system tissue preferred. The individual will work with only
minimum supervision.

Position Requirements

Minimum of five years experience working in a laboratory with hands-on
experience in tissue processing, histochemistry, immunohistochemistry,
and operation of a light microscope. Experience with technical aspects of
neuropathology and performing special stains including silver
(Bielschowsky) and myelin stains.

Two years experience working on immunohistochemistry of CNS tissue
specimens with knowledge of immunoreagents and experimental protocols.

BachelorUs degree in science desirable.

Laboratory skills including communications, ability to comply with safety
and laboratory regulations, maintenance of laboratory equipment and
resources, operation of computers and office equipment.

Advanced computer skills (word processing and database management) essential.

Desirable Experience

Confocal microscopy desirable.

Salary commensurate with experience


Responsibilities

Purchasing supplies and equipment, budget reports, laboratory maintenance
and brain banking.

Will operate all microscopic, photographic and computer equipment, and
keep accurate records of all laboratory experiments and procedures. Light
and fluorescent microscopy; tissue processing for paraffin embedding,
sectioning and slide stainer for immunohistochemical procedures; computer
imaging (PhotoShop); general photography; and library and web searches

Familiarity with computer software for keeping records, report
preparation and table/figure construction using Microsoft Office software
(Word, Excel, PowerPoint) as well as Endnote and Adobe PhotoShop.

Send resume to:

Suzanne Mirra, M.D., Chair
Department of Pathology
Box 25
SUNY-Brooklyn
450 Clarkson Av.
Brooklyn, NY 11203-2098

-------------------
Submitted by
Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265





From daemon Mon Nov 6 16:49:05 2000



From: Sara Miller :      saram-at-duke.edu
Date: Mon, 6 Nov 2000 17:36:26 -0500 (EST)
Subject: EM Tech Position Open

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Experienced Electron Microscopy Technologist to work in Research
Laboratory of Department of Pathology


The person we seek will be responsible for the overall operation of the
EM laboratory in the Department of Pathology at SUNY Downstate Medical
Center. This individual will oversee all aspects of specimen accession
and processing, operation of the microscope, photography, record keeping,
and supervision of a technician. Knowledge of EM, biology, and pathology,
as well as photographic procedures is required. The individual will work
with only minimum supervision.

Position Requirements

Minimum of five years experience working in an electron microscopy
laboratory with hands-on experience in tissue processing, dark room
photography, and operation and maintenance of electron microscope.

Two years experience working on electron microscopy of human tissue
specimens with knowledge of histology and pathology.

BachelorUs degree in science desirable.

Laboratory management skills including effective written/verbal
communication skills to interact with a diverse group, ability to comply
with safety and laboratory regulations, maintenance of laboratory
equipment and resources, and operation of computers and office equipment.

Desirable Experience

Previous experience in confocal microscopy highly desirable.

Previous experience in performing immunocytochemical staining and
advanced computer skills usage (e.g. image analysis) is also desirable.

Salary commensurate with experience

Responsibilities

Maintain electron microscope in operating condition. Process clinical and
research tissues for "thick" and "thin" sectioning. Darkroom management
of photographic printing.


Send resume to:
Suzanne Mirra, M.D., Chair
Department of Pathology
Box 25
SUNY-Brooklyn
450 Clarkson Av.
Brooklyn, NY 11203-2098

-----------------
Submitted by:
Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265





From daemon Mon Nov 6 18:10:54 2000



From: Kun Li :      k-li-at-mailcityasia.com
Date: Tue, 07 Nov 2000 07:58:22 +0800
Subject: Sound absorption curtain

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers,

We are going to install a curtain for sound absorption in our TEM Lab. Is there any special curtain for this purpose?

Regards,

Kun Li


Get your FREE Email at http://www.mailcityasia.com



From daemon Mon Nov 6 18:31:05 2000



From: Nestor J. Zaluzec :      zaluzec-at-aaem.amc.anl.gov
Date: Mon, 6 Nov 2000 18:29:09 -0600
Subject: Re: Sound absorption curtain

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



In many of the microscope rooms here at ANL. we
use a product called SONEX by illbruck (http://www.illbruck.com). It is both fire
rated and works reasonably well. Log into the TPM WWW site
(http://tpm.amc.anl.gov) and you can see it . It is an acoustic foam panel which
glues to the walls of the lab. The only problem is that being a foam
product it tends to easily tear/rip if it is hit by objects.

Nestor




} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


==================================================================
Dr. Nestor J. Zaluzec
Materials Science Division
Building 212
Argonne National Lab
9700 S. Cass Ave
Argonne, Illinois 60439 USA
Tel: 630-252-7901, Fax: 630-252-4798
Email: Zaluzec-at-aaem.amc.anl.gov
==================================================================
TPMLab: http://tpm.amc.anl.gov
MMSite: http://www.amc.anl.gov
==================================================================

The box said "This program requires Win 95/98/NT or better..."
So I bought a G3 Mac !

==================================================================





From daemon Mon Nov 6 19:54:42 2000



From: Kalman Rubinson :      kr4-at-is2.nyu.edu
Date: Mon, 6 Nov 2000 20:51:03 -0500 (EST)
Subject: Re: Sound absorption curtain

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On Tue, 7 Nov 2000, Kun Li wrote:

} We are going to install a curtain for sound absorption
} in our TEM Lab. Is there any special curtain for this
} purpose?

What sort of noise? For mid- to high-frequencies, a heavy
fabric drape, interlined with an absorbent material, will do
a pretty good job. For low frequencies, no curtain will do
as you need a membrane with a seal.

Kal




From daemon Mon Nov 6 20:37:48 2000



From: Kalman Rubinson :      kr4-at-is2.nyu.edu
Date: Mon, 6 Nov 2000 21:35:05 -0500 (EST)
Subject: Re: Sound absorption curtain

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On Mon, 6 Nov 2000, Nestor J. Zaluzec wrote:
} In many of the microscope rooms here at ANL. we
} use a product called SONEX by illbruck (http://www.illbruck.com). It is both fire
} rated and works reasonably well. Log into the TPM WWW site
} (http://tpm.amc.anl.gov) and you can see it . It is an acoustic foam panel which
} glues to the walls of the lab. The only problem is that being a foam
} product it tends to easily tear/rip if it is hit by objects.

This will do nothing to keep sound out. It will absorb
high frequencies within the room.

Better sites for information and materials are Acoustic
Sciences Corporation and Auralex.

Kal






From daemon Mon Nov 6 20:40:45 2000



From: =?iso-8859-1?Q?Mar=EDa?= Alejandra Maine :      amaine-at-fiqus.unl.edu.ar
Date: Mon, 6 Nov 2000 20:38:38 -0600
Subject: suggestions for SEM/aquatic plants/fish

Contents Retrieved from Microscopy Listserver Archives
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Hello everyone,
I was wondering if anyone had suggestions on a
protocol for the fixation of either aquatic plant tissue or fish tissue
to be viewed in the SEM. My interest is to study the sorption mechanisms of
heavy metals (Cr, Cd and Pb) by aquatic plants and the bioaccumulation in
fishes.
I have no experience on SEM, so any information will be very usefull for me.
Thanks,
Regards!

Alejandra Maine





From daemon Mon Nov 6 23:14:13 2000



From: Corazon D. Bucana :      bucana-at-audumla.mdacc.tmc.edu
Date: Mon, 06 Nov 2000 23:04:40 -0500
Subject: fluorescence of collagen

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all,
I would appreciate any suggestions or comments on how to suppress
fluorescence of collagen in frozen sections that have been subjected to
indirect immunofluorescence using Alexa conjugated antibodies. I have used
normal goat and normal horse serum to block non-specific staining as well
as 4 % fish gelatin but the collagen glows brightly every time. the
samples were fixed in cold acetone before blocking. Incubation with sodium
borohydride did not reduce fluorescence.

Thanks,

Cora Bucana
Corazon D. Bucana, Ph.D.
Dept. Cancer Biology
UT M.D.Anderson Cancer Center
1515 Holcombe Blvd, Box 173
Houston, Texas 77030
Phone: 713-792-8106
FAX 713-792-8747



From daemon Tue Nov 7 02:09:10 2000



From: Chris Jeffree :      cjeffree-at-srv0.bio.ed.ac.uk
Date: Tue, 7 Nov 2000 07:57:54 -0000
Subject: Re: Sound absorption curtain

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Kal
could you expand on this a little? What type of membrane, arranged
how?, etc. What range of frequencies can be controlled this way?
I am looking for a relatively straightforward and inexpensive way of
controlling particularly the low end of the spectrum of acoustic
frequencies (generated by human voice, RVPs, etc.) in an FESEM
lab.
Chris

} For low frequencies, no curtain will do
} as you need a membrane with a seal.
}
} Kal
}
}


~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Dr Chris Jeffree
University of Edinburgh
Biological Sciences EM Facility
Daniel Rutherford Building
King's Buildings EDINBURGH EH9 3JH
Tel: +44 (0) 131 650 5345
FAX: +44 (0) 131 650 6563

Inveresk Cottage, 26 Carberry Road,
Inveresk, Musselburgh, Midlothian EH21 8PR, UK
Tel: +44 (0) 131 665 6062 / Mobile 07710 585 401
FAX: +44 (0) 131 653 6248
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~



From daemon Tue Nov 7 03:09:15 2000



From: Gordon Couger :      gcouger-at-couger.com
Date: Tue, 7 Nov 2000 03:06:25 -0600
Subject: Re: Sound absorption curtain

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Nestor

I have found that if I keep some scraps around after the original
installation of almost anything you can mix up a little glue wiht it and
make a passable fix. Some times you have to do a little painting and make
a creative dirty spot.

You will always be able to see it but no one else will.

If you are worried about getting the texture right use cheap wall paper
paste and you can wash it out if you don't like it but it has enough water
resistance to resist minor spills.

Test it where it won't show first.

Gordon
Gordon Couger gcouger-at-couger.com
Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00
}
} In many of the microscope rooms here at ANL. we
} use a product called SONEX by illbruck (http://www.illbruck.com). It is
both fire
} rated and works reasonably well. Log into the TPM WWW site
} (http://tpm.amc.anl.gov) and you can see it . It is an acoustic foam
panel which
} glues to the walls of the lab. The only problem is that being a foam
} product it tends to easily tear/rip if it is hit by objects.
}
} Nestor
}
}
}
}
}
} ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
} } On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
}
} -----------------------------------------------------------------------.
} }
} }
} } Dear Listers,
} }
} } We are going to install a curtain for sound absorption in our TEM Lab.
Is there any special curtain for this purpose?
} }
} } Regards,
} }
} } Kun Li
} }
} }
} } Get your FREE Email at http://www.mailcityasia.com
}
}
} ==================================================================
} Dr. Nestor J. Zaluzec
} Materials Science Division
} Building 212
} Argonne National Lab
} 9700 S. Cass Ave
} Argonne, Illinois 60439 USA
} Tel: 630-252-7901, Fax: 630-252-4798
} Email: Zaluzec-at-aaem.amc.anl.gov
} ==================================================================
} TPMLab: http://tpm.amc.anl.gov
} MMSite: http://www.amc.anl.gov
} ==================================================================
}
} The box said "This program requires Win 95/98/NT or better..."
} So I bought a G3 Mac !
}
} ==================================================================
}
}
}





From daemon Tue Nov 7 05:03:07 2000



From: Bart De Pauw :      Bart.DePauw-at-rug.ac.be
Date: Tue, 07 Nov 2000 11:57:42 +0100
Subject: SEM - Apoptotic cells

Contents Retrieved from Microscopy Listserver Archives
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Hello listers,

We are experimenting with apoptose. Now, we want to view apoptotic cells
under the SEM. Has anyone ideas what technique, solutions,....we have
to use ? The sample is canine ovary.

Thx.

Bart De Pauw
Ghent University
Faculty of Veterinary medicine
Department Morphology
Salisburylaan 133
9820 Merelbeke
Belgium




From daemon Tue Nov 7 07:48:30 2000



From: Kim Riddle :      riddle-at-bio.fsu.edu
Date: Tue, 07 Nov 2000 09:08:47 -0500
Subject: job listing

Contents Retrieved from Microscopy Listserver Archives
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Senior Biological Scientist: Florida USPS grade 28 BS/MS level

Description of Duties: Works with the Coordinator of Research
Programs/Services in the management of the day-to-day activities of the
Biological Science Imaging Resource, a campus wide imaging laboratory. This
facility is responsible for assisting faculty and students in the
preparation of biological images for research, publication and instruction.
The Senior Biological Scientist works closely with the Coordinator to
identify facility needs, establish priorities and develop policies within
the laboratory as well as instruct users in the operation of the electron
and light, including confocal microscopes. The incumbent must stay abreast
of research standards and practices to maintain quality control and advise
users on appropriate protocols. Knowledge in the use of MetaMorph,
MetaFluor, Photoshop and PowerPoint along with MAC and PC platforms is a
plus.

Contact Kim Riddle, 119 Bio Unit I, Department of Biological Science,
Florida State University, Tallahassee, FL 32306-4370, riddle-at-bio.fsu.edu
850.644.6519
Imaging Resource web page http://bio.fsu.edu/~taylor/imaging
On-line applications available at http://personnel.fsu.edu/emply/homepage.html

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Kimberly A. Riddle
Florida State University tel: 850.644.6519
Biological Science Imaging Resource
119 Bio Unit I, 4370 fax: 850.644.0481
Tallahassee, FL 32306 riddle-at-bio.fsu.edu
http://bio.fsu.edu/~taylor/imaging
~~~~~~~~~~~~~~~~~~~~~~~~~~~~




From daemon Tue Nov 7 07:48:30 2000



From: Yi Liu :      yliu-at-unlserve.unl.edu
Date: Tue, 7 Nov 2000 07:43:30 -0600
Subject: Position for a visiting scholar in HRTEM image processing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Colleague:

The Center for Materials Research and Analysis (CMRA) at the
University of Nebraska at Lincoln has a matching fund in connection with a
NSF award for a person to work with Prof. Y. Liu and his co-PIs on image
processing of high resolution electron micrographs. The method developed
will be applied to the TEM characterization of various nanostructured
materials, such as TiN coatings, nanowires, super-conducting materials,
recording media and permanent magnets. The initial appointment will be one
year and can be renewed the second year, depending upon progress and
availability of funding. Computer skills, knowledge of Fourier transform
and image processing are required.
Interested persons should email a letter of interest, resume and
three persons of references to yliu-at-unlserve.unl.edu--
*******************************************************************
Yi Liu
Department of Mechanical Eng. and CMRA
104 N Walter Scott Engineering Center
University of Nebraska-Lincoln
Lincoln, NE 68588-0656
Tel. (402) 472-7759 (Office)
Tel. (402) 472-8762 (EM lab)
Fax (402) 472-1465
Email: yliu-at-unlserve.unl.edu
*******************************************************************





From daemon Tue Nov 7 09:14:33 2000



From: Kalman Rubinson :      kr4-at-is2.nyu.edu
Date: Tue, 7 Nov 2000 10:05:41 -0500 (EST)
Subject: Re: Sound absorption curtain

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On Tue, 7 Nov 2000, Chris Jeffree wrote:

} could you expand on this a little? What type of membrane, arranged
} how?, etc. What range of frequencies can be controlled this way?
} I am looking for a relatively straightforward and inexpensive way of
} controlling particularly the low end of the spectrum of acoustic
} frequencies (generated by human voice, RVPs, etc.) in an FESEM
} lab.

Assuming that you want to keep external noise out, you need
to block the window since the glass transmits a lot.
Hanging drapes, of any kind, will be most effective at
higher frequencies where they can absorb energy transmitted
by the glass.

If you can seal over the window with a membrane that will
absorb at low frequencies (and you must seal it for it to
be effective at low frequencies), take a look at SheetBlok
at http://www.auralex.com/. This is effective down into the
range of about 100Hz. Alternatively, you can brick up the
window.

Kal




From daemon Tue Nov 7 09:17:32 2000



From: Larry Allard :      l2a-at-ornl.gov
Date: Tue, 07 Nov 2000 07:55:26 -0500
Subject: Re: Sound absorption curtain

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Kun Li:

We also use Sonex foam products in most of our laboratories, but are
now looking at purchasing an "enclosure" to put around a noisy water
chiller. A company called Sound Seal (www.soundseal.com) makes such
enclosures (at *very* reasonable prices), and has fabric curtains and
panels for sound absorption purposes. Check them out...

Larry





} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Dr. Lawrence F. Allard
Senior Research Staff Member
High Temperature Materials Laboratory
Oak Ridge National Laboratory
1 Bethel Valley Road
Bldg. 4515, MS 6064
PO Box 2008
Oak Ridge, TN 37831-6064

865-574-4981
865-576-5413 Fax
allardlfjr-at-ornl.gov



From daemon Tue Nov 7 10:11:58 2000



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Tue, 07 Nov 2000 10:07:53 -0600
Subject: Re: Sound absorption curtain

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


You might need to expand a bit on your goal. It seems that you are
indicating that human speech is degrading your resolution. I find that hard
to believe. Pumps, maybe. All these sources would depend on their manner of
coupling to the scope. I don't think that speech would be that effectively
coupled to be a problem.

Have you tried collecting some images where part of the image has the
source present and part does not? I would think you could even shut down
your rotary pumps for long enough to determine their influence on imaging.
Take appropriate precautions.

Like the previous discussion on mu metal shielding, it seems that you might
want to isolate the problem better and/or try some cheap and crude
solutions before investing the bigger bucks.

FWIW,
Warren Straszheim

At 07:57 AM 11/7/2000 +0000, you wrote:

} Kal
} could you expand on this a little? What type of membrane, arranged
} how?, etc. What range of frequencies can be controlled this way?
} I am looking for a relatively straightforward and inexpensive way of
} controlling particularly the low end of the spectrum of acoustic
} frequencies (generated by human voice, RVPs, etc.) in an FESEM
} lab.
} Chris
}
} } For low frequencies, no curtain will do
} } as you need a membrane with a seal.
} }
} } Kal
} ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
} Dr Chris Jeffree
} University of Edinburgh
} Biological Sciences EM Facility
} Daniel Rutherford Building
} King's Buildings EDINBURGH EH9 3JH
} Tel: +44 (0) 131 650 5345
} FAX: +44 (0) 131 650 6563




From daemon Tue Nov 7 10:34:07 2000



From: Robert Palmer :      rjpalmer-at-dir.nidcr.nih.gov
Date: Tue, 7 Nov 2000 11:35:58 -0500
Subject: 3d recon and measurement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am interested in acquiring software for 3D image reconstruction and
measurement (distance/volume) in 3D space. What is the current state of
the market in this area? I am familiar with only one company that provides
such a package (Vital Images: VoxelView), but I bet there are others. No
need to evangelize - just let me know what other products I should examine.
Money is not a big obstacle, I do not want to write code myself (I want a
turnkey solution), and I do not require image acquisition or 2D analysis
features.
Thanks for the input.
Robert J. Palmer Jr., Ph.D.
Natl Inst Dental Craniofacial Res - Natl Insts Health
Oral Infection and Immunity Branch
Bldg 30, Room 308
30 Convent Drive
Bethesda MD 20892
ph 301-594-0025
fax 301-402-0396



From daemon Tue Nov 7 12:41:16 2000



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Tue, 7 Nov 2000 08:37:19 -1000 (HST)
Subject: Re: Sound absorption curtain

Contents Retrieved from Microscopy Listserver Archives
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Speech can indeed be a problem with FESEMs. In the case of our instrument,
the shrouding around the ion pumps behind the column was acting as a drum,
transmitting speech particularly well. I removed the shrouding and the
problem was eliminated. Plus now the scope looks much more interesting
and impresses visitors! In newer models from the same company the
shrouding is now perforated to minimize the problem.

} You might need to expand a bit on your goal. It seems that you are
} indicating that human speech is degrading your resolution. I find that hard
} to believe. Pumps, maybe. All these sources would depend on their manner of
} coupling to the scope. I don't think that speech would be that effectively
} coupled to be a problem.

Aloha,
Tina

Mid-80s F, sunny with tropical breezes

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************




From daemon Tue Nov 7 14:10:05 2000



From: Chris Jeffree :      cjeffree-at-srv0.bio.ed.ac.uk
Date: Tue, 7 Nov 2000 19:59:28 -0000
Subject: Re: Sound absorption curtain

Contents Retrieved from Microscopy Listserver Archives
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Yes I am indicating exactly that. Certain frequencies of the human
voice at normal conversational amplitudes are picked up by FESEM
columns and can induce saw-tooth edges on sharp-edged features
(such as gold crystals on carbon) at magnifications in the x100k
region. Trust me on this. It is a real problem. If one is contemplating
high resolution work with an FESEM a hushed environment is
essential.

Chris

} It seems that you are
} indicating that human speech is degrading your resolution. I find that hard
} to believe. Pumps, maybe. All these sources would depend on their manner of
} coupling to the scope. I don't think that speech would be that effectively
} coupled to be a problem.
}
} Have you tried collecting some images where part of the image has the
} source present and part does not? I would think you could even shut down
} your rotary pumps for long enough to determine their influence on imaging.
} Take appropriate precautions.
}
} Like the previous discussion on mu metal shielding, it seems that you might
} want to isolate the problem better and/or try some cheap and crude
} solutions before investing the bigger bucks.
}
} FWIW,
} Warren Straszheim
}
} At 07:57 AM 11/7/2000 +0000, you wrote:
}
} } Kal
} } could you expand on this a little? What type of membrane, arranged
} } how?, etc. What range of frequencies can be controlled this way?
} } I am looking for a relatively straightforward and inexpensive way of
} } controlling particularly the low end of the spectrum of acoustic
} } frequencies (generated by human voice, RVPs, etc.) in an FESEM
} } lab.
} } Chris
} }
} } } For low frequencies, no curtain will do
} } } as you need a membrane with a seal.
} } }
} } } Kal
} } ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
} } Dr Chris Jeffree
} } University of Edinburgh
} } Biological Sciences EM Facility
} } Daniel Rutherford Building
} } King's Buildings EDINBURGH EH9 3JH
} } Tel: +44 (0) 131 650 5345
} } FAX: +44 (0) 131 650 6563
}
}


~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Dr Chris Jeffree
University of Edinburgh
Biological Sciences EM Facility
Daniel Rutherford Building
King's Buildings EDINBURGH EH9 3JH
Tel: +44 (0) 131 650 5345
FAX: +44 (0) 131 650 6563

Inveresk Cottage, 26 Carberry Road,
Inveresk, Musselburgh, Midlothian EH21 8PR, UK
Tel: +44 (0) 131 665 6062 / Mobile 07710 585 401
FAX: +44 (0) 131 653 6248
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~



From daemon Tue Nov 7 14:27:36 2000



From: Marek Malecki :      mmm-at-ucsd.edu
Date: Tue, 07 Nov 2000 12:27:05 -0800
Subject: Pfeiffer's no-oil vacuum pumps

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I have 2 Pfeiffer's no-oil vacuum pumps for 8m3/min to offer . They have
only 50hours of work. The original price the last year was $6400. Any offer
negotiable.
Marek Malecki, M.D., Ph.D.
Director and Principal Investigator

Molecular Imaging Laboratories
University of California, San Diego

address: 1500 Suite Bonner Hall, 9500 Gilman Drive, La Jolla, CA92093-0368
phone - office: 8588223373
phone - cell: 8583443347
fax: 8588223715
e.mail: mmm-at-ucsd.edu
pager: 8586161420
e.mail: mmm-at-ucsd.edu
e.pager: 1620024619-at-alphapage.airtouch.com
www site: http://mil.ucsd.edu/
ftp site: mil.ucsd.edu





From daemon Tue Nov 7 14:57:52 2000



From: William Tivol :      tivol-at-wadsworth.org
Date: Tue, 7 Nov 2000 15:48:11 -0500 (EST)
Subject: Re: Sound absorption curtain

Contents Retrieved from Microscopy Listserver Archives
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Warren E Straszheim:
}
} You might need to expand a bit on your goal. It seems that you are
} indicating that human speech is degrading your resolution. I find that hard
} to believe.

Dear Warren,
I still remember Ken Downing demonstrating that his IVEM
image would vibrate whenever one talked at the microscope--in fact,
at a DOE site visit, the head of the team, a Dr. Happer, seemed
singularly unimpressed until he was shown that clapping in the
IVEM room would cause the image to vibrate. He was apparently
quite amused, and the site visit went well after that. The IVEM
was known for a time as the "Happer clapper".
Yours,
Bill Tivol
Wadsworth Center
Albany NY



From daemon Tue Nov 7 15:06:37 2000



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Tue, 7 Nov 2000 15:02:33 -0600
Subject: RE: Sound absorption curtain

Contents Retrieved from Microscopy Listserver Archives
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I have to back Chris up on this. We have often seen voice-induced vibration
in our FESEM. No talking during high-mag, high-res work.

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/



------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Yes I am indicating exactly that. Certain frequencies of the human
voice at normal conversational amplitudes are picked up by FESEM
columns and can induce saw-tooth edges on sharp-edged features
(such as gold crystals on carbon) at magnifications in the x100k
region. Trust me on this. It is a real problem. If one is contemplating
high resolution work with an FESEM a hushed environment is
essential.

Chris

} It seems that you are
} indicating that human speech is degrading your resolution. I find that
hard
} to believe. Pumps, maybe. All these sources would depend on their manner
of
} coupling to the scope. I don't think that speech would be that effectively

} coupled to be a problem.
}
} Have you tried collecting some images where part of the image has the
} source present and part does not? I would think you could even shut down
} your rotary pumps for long enough to determine their influence on imaging.

} Take appropriate precautions.
}
} Like the previous discussion on mu metal shielding, it seems that you
might
} want to isolate the problem better and/or try some cheap and crude
} solutions before investing the bigger bucks.
}
} FWIW,
} Warren Straszheim
}
} At 07:57 AM 11/7/2000 +0000, you wrote:
}
} } Kal
} } could you expand on this a little? What type of membrane, arranged
} } how?, etc. What range of frequencies can be controlled this way?
} } I am looking for a relatively straightforward and inexpensive way of
} } controlling particularly the low end of the spectrum of acoustic
} } frequencies (generated by human voice, RVPs, etc.) in an FESEM
} } lab.
} } Chris
} }
} } } For low frequencies, no curtain will do
} } } as you need a membrane with a seal.
} } }
} } } Kal
} } ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
} } Dr Chris Jeffree
} } University of Edinburgh
} } Biological Sciences EM Facility
} } Daniel Rutherford Building
} } King's Buildings EDINBURGH EH9 3JH
} } Tel: +44 (0) 131 650 5345
} } FAX: +44 (0) 131 650 6563
}
}


~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Dr Chris Jeffree
University of Edinburgh
Biological Sciences EM Facility
Daniel Rutherford Building
King's Buildings EDINBURGH EH9 3JH
Tel: +44 (0) 131 650 5345
FAX: +44 (0) 131 650 6563

Inveresk Cottage, 26 Carberry Road,
Inveresk, Musselburgh, Midlothian EH21 8PR, UK
Tel: +44 (0) 131 665 6062 / Mobile 07710 585 401
FAX: +44 (0) 131 653 6248
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~



From daemon Tue Nov 7 16:08:00 2000



From: Kalman Rubinson :      kr4-at-is2.nyu.edu
Date: Tue, 7 Nov 2000 17:03:33 -0500 (EST)
Subject: Re: Sound absorption curtain

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On Tue, 7 Nov 2000, Chris Jeffree wrote:

} Yes I am indicating exactly that. Certain frequencies of the human
} voice at normal conversational amplitudes are picked up by FESEM
} columns and can induce saw-tooth edges on sharp-edged features
} (such as gold crystals on carbon) at magnifications in the x100k
} region. Trust me on this. It is a real problem. If one is contemplating
} high resolution work with an FESEM a hushed environment is
} essential.

OK. Where are these voices? In the room? If not, the
issue is to keep them out. Perhaps you should contact a
number of the specialist firms and consult with them via
their websites. I have gotten very useful advice and
information without (or prior to) purchase of sound
treatment materials.

Kal

} } It seems that you are
} } indicating that human speech is degrading your resolution. I find that hard
} } to believe. Pumps, maybe. All these sources would depend on their manner of
} } coupling to the scope. I don't think that speech would be that effectively
} } coupled to be a problem.
} }
} } Have you tried collecting some images where part of the image has the
} } source present and part does not? I would think you could even shut down
} } your rotary pumps for long enough to determine their influence on imaging.
} } Take appropriate precautions.
} }
} } Like the previous discussion on mu metal shielding, it seems that you might
} } want to isolate the problem better and/or try some cheap and crude
} } solutions before investing the bigger bucks.
} }
} } FWIW,
} } Warren Straszheim
} }
} } At 07:57 AM 11/7/2000 +0000, you wrote:
} }
} } } Kal
} } } could you expand on this a little? What type of membrane, arranged
} } } how?, etc. What range of frequencies can be controlled this way?
} } } I am looking for a relatively straightforward and inexpensive way of
} } } controlling particularly the low end of the spectrum of acoustic
} } } frequencies (generated by human voice, RVPs, etc.) in an FESEM
} } } lab.
} } } Chris
} } }
} } } } For low frequencies, no curtain will do
} } } } as you need a membrane with a seal.
} } } }
} } } } Kal
} } } ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
} } } Dr Chris Jeffree
} } } University of Edinburgh
} } } Biological Sciences EM Facility
} } } Daniel Rutherford Building
} } } King's Buildings EDINBURGH EH9 3JH
} } } Tel: +44 (0) 131 650 5345
} } } FAX: +44 (0) 131 650 6563
} }
} }
}
}
} ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
} Dr Chris Jeffree
} University of Edinburgh
} Biological Sciences EM Facility
} Daniel Rutherford Building
} King's Buildings EDINBURGH EH9 3JH
} Tel: +44 (0) 131 650 5345
} FAX: +44 (0) 131 650 6563
}
} Inveresk Cottage, 26 Carberry Road,
} Inveresk, Musselburgh, Midlothian EH21 8PR, UK
} Tel: +44 (0) 131 665 6062 / Mobile 07710 585 401
} FAX: +44 (0) 131 653 6248
} ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
}






From daemon Tue Nov 7 17:06:20 2000



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Tue, 07 Nov 2000 16:57:40 -0600
Subject: Re: Sound absorption curtain

Contents Retrieved from Microscopy Listserver Archives
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Well, I guess I learned something today. I usually show operators that loud
noises will show up as low energy noise in the EDS spectra. I had no idea
that it also affected the images that much. We do not do that much work
above 10 kx. I will have to bear that in mind the next time we venture into
that realm and turn down the 1812 Overture in the background. I may have to
quiet our pumps down too.

Warren

At 07:59 PM 11/7/2000 +0000, you wrote:
} Yes I am indicating exactly that. Certain frequencies of the human
} voice at normal conversational amplitudes are picked up by FESEM
} columns and can induce saw-tooth edges on sharp-edged features
} (such as gold crystals on carbon) at magnifications in the x100k
} region. Trust me on this. It is a real problem. If one is contemplating
} high resolution work with an FESEM a hushed environment is
} essential.
}
} Chris




From daemon Tue Nov 7 17:12:04 2000



From: Jaap Brink :      brink-at-tiger.3dem.bioch.bcm.tmc.edu
Date: Tue, 7 Nov 2000 17:09:41 -0600 (CST)
Subject: Re: Sound absorption curtain

Contents Retrieved from Microscopy Listserver Archives
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On Tue, 7 Nov 2000, Kalman Rubinson wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} On Tue, 7 Nov 2000, Chris Jeffree wrote:
}
} } Yes I am indicating exactly that. Certain frequencies of the human
} } voice at normal conversational amplitudes are picked up by FESEM
} } columns and can induce saw-tooth edges on sharp-edged features
} } (such as gold crystals on carbon) at magnifications in the x100k
} } region. Trust me on this. It is a real problem. If one is contemplating
} } high resolution work with an FESEM a hushed environment is
} } essential.
}
} OK. Where are these voices? In the room? If not, the
} issue is to keep them out. Perhaps you should contact a
} number of the specialist firms and consult with them via
} their websites. I have gotten very useful advice and
} information without (or prior to) purchase of sound
} treatment materials.
}
} Kal
}
(snip)

I'd like to jump into this one by adding my $0.02. I'm familiair with Ken
Downing's results related to vibrations. We tried it ourselves as well
and microphonics are indeed a very real problem when one does HR work. The
effects are indeed very clearly visible on the TV screen at high (} =
100kx) mag. Ken (together with Bob Glaeser) and we (perhaps others
have done something similar) have taken this a step further and
constructed boxes made of styrofoam that fit around the cryo-holder (side
entry types). The idea was to keep microphonics away from the holder
including those created by us talking or on the phone or listening to a
radio, but also those originating from pumps, ventilation and/or air
conditioning systems.

Jaap

-- --
Jaap Brink, Ph.D., Biochemistry, One Baylor Plaza, Baylor College of
Medicine, Rm. N420 Alkek Building, Houston, TX 77030
Phone: (713)798-6989 -- Fax: (713)796-9438 -- Email: jbrink-at-bcm.tmc.edu
URL : http://ncmi.bioch.bcm.tmc.edu/~brink




From daemon Tue Nov 7 18:41:46 2000



From: Dave Audette :      deaudette-at-yahoo.com
Date: Tue, 7 Nov 2000 16:36:34 -0800 (PST)
Subject: EDS networking

Contents Retrieved from Microscopy Listserver Archives
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Hello listers,

I operate a Noran Voyager 3.3 EDS system, which is a
UNIX based system and operates on a Sun Sparc Station
5 with SunOS 5.3. The problem is the local IT
department is reluctant to place this on the network
which is all PC based. The IT staff feels it would be
more cost efficient use of their time, if I found any
product which will replace the Unix box/software and
utilize the present hardware (detector, amplifiers,
etc.). Anyone have any experience with this?

TIA,

Dave Audette
OSRAM Sylvania
71 Cherry Hill Drive
Beverly, MA 01915
david.audette-at-sylvania.com



__________________________________________________
Do You Yahoo!?
Thousands of Stores. Millions of Products. All in one Place.
http://shopping.yahoo.com/



From daemon Tue Nov 7 19:23:53 2000



From: Bruce Cutler :      bcutler-at-eureka.idl.ukans.edu
Date: Tue, 7 Nov 2000 19:20:56 -0600
Subject: Schottky vs. cold FESEM

Contents Retrieved from Microscopy Listserver Archives
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Thanks to all who responded to my questions.
Bruce
Bruce Cutler
Director, Microscopy & Electronic Imaging Laboratory
University of Kansas





From daemon Tue Nov 7 20:32:18 2000



From: Thearith H. Ung :      tung-at-qdots.com
Date: Tue, 7 Nov 2000 18:25:37 -0800
Subject: TEM CCD Camera/EDS

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Hi Everybody,

Does anybody know of any used CCD camera and EDS in good condition that are
up for sail? I would like to purchase these to use on a used JEOL 200CX TEM.

Your help would be greatly appreciated.

Regards,
Thearith

_________________________

Thearith Ung, Ph.D.
Quantum Dot Corporation
26136 Research Road
Hayward CA 94545
Tel: +1-510-887-8775 (x4125)
Fax:+1-510-783-9729
www.qdots.com




From daemon Tue Nov 7 20:57:32 2000



From: Scott D. Davilla :      davilla-at-4pi.com
Date: Tue, 7 Nov 2000 21:51:45 -0500
Subject: Re: EDS networking

Contents Retrieved from Microscopy Listserver Archives
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} I operate a Noran Voyager 3.3 EDS system, which is a
} UNIX based system and operates on a Sun Sparc Station
} 5 with SunOS 5.3. The problem is the local IT
} department is reluctant to place this on the network
} which is all PC based. The IT staff feels it would be
} more cost efficient use of their time, if I found any
} product which will replace the Unix box/software and
} utilize the present hardware (detector, amplifiers,
} etc.). Anyone have any experience with this?

Your IT department is over simplifying the cost. They most likey
think it's only 1 or 2k to get a WinOS computer. They are very wrong.

Cost is really going to be from ~10k to ~20k to replace the Voyager
depending if you go with a Noran (Quest) replacement or some other like us.
You will be able to keep the detector (although maybe a preamp change) but
everything else is replaced. Also you will have to learn new EDS software
(your time at two to three weeks). In addition, all your old data and
analysis might or might not be movable to the new EDS system (how much is
your data worth).

Tell your managment that your IT department needs to to support you
rather than you supporting them, after all, what is the function of the TI
department. The Voyager is a plain UNIX box, if no one in the IT department
understands UNIX enough to network it, they are pretty sorry.
If that does not work, then tell then you will be glad to replace
the UNIX with a WinOS equivalent provided they pony up the funds. Also
remind managment that you will get no real work done while you learn the
new software. Add the fact that previous data results might be forever lost
to access.
Let them figure that the IT deparment is going to cost the company
10-20k plus the cost of your time because they can't seem to do their job
which is to support the computing needs of the workers.
Bottom line is that it's going to cost the company 40-60k direct
and indirect costs however if you can't move the data, then it could cost
the company big time if they need to access it in the future. Now what is
really more cost efficient?

Sorry for the rant, but it really bugs me when IT departments
operate in such a priesthood fashion, we run into it all the time with our
MacOS products so much that we came out with WinOS products for those that
have to have WinOS because of stupid IT restrictions.

Scott
-----------------------------------------------------------------------
Scott D. Davilla Phone: 919 489-1757 ext 13 (tel)
4pi Analysis, Inc. Fax: 919 489-1487 (fax)
3500 Westgate Drive, Suite 403 email: davilla-at-4pi.com
Durham, North Carolina 27707-2534 web: http://www.4pi.com




From daemon Tue Nov 7 23:29:27 2000



From: Gary Gaugler :      gary-at-gaugler.com
Date: Tue, 07 Nov 2000 21:24:00 -0800
Subject: Re: EDS networking

Contents Retrieved from Microscopy Listserver Archives
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Huh? What PCs? If they are all DOS-based, I can identify
with their concerns. If the PCs are running client TCP/IP,
the addition of the Sun should be trivial.

How are LAN IPs assigned? If there is a LAN NAT
server, then just assign the Sun a within-scope IP address.
If it is DHCP, just do it.

I don't see what the big deal is. Except....I'll venture
to say that the PC jockeys have no Unix experience.
That would be a good reason to keep the Sun away
from the PC LAN.

gg


At 04:36 PM 11/7/00, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From daemon Wed Nov 8 01:54:05 2000



From: Gordon Couger :      gcouger-at-couger.com
Date: Tue, 7 Nov 2000 13:52:06 -0600
Subject: Re: EDS networking

Contents Retrieved from Microscopy Listserver Archives
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} From: "Scott D. Davilla" {davilla-at-4pi.com}
}
} } I operate a Noran Voyager 3.3 EDS system, which is a
} } UNIX based system and operates on a Sun Sparc Station
} } 5 with SunOS 5.3. The problem is the local IT
} } department is reluctant to place this on the network
} } which is all PC based. The IT staff feels it would be
} } more cost efficient use of their time, if I found any
} } product which will replace the Unix box/software and
} } utilize the present hardware (detector, amplifiers,
} } etc.). Anyone have any experience with this?
===========
Just tell them you can't get fries wiht that and do their job. And don't
hold your breath. If you ask around there may be a couple working wiht
Linux and they can figure it out.

Just borrow some one with unix experiance to set it up from another
department for a favor to be anouced later and let the IT department spin
on it. If they are windows based the box will weld it's self shut by
molecular defusion before they take the time to figure out unix.

Hiring a consultant is a reasonable soluting if a college is near. Once it
is set up it can be maintained from any place in the world. Unless you are
adding stuff a couple of hours a month will do the job.

The best answer is some one in the shop learn to do it. It will take some
time but it will pay off in he long run.

Good luck You need it.
Gordon
Gordon Couger gcouger-at-couger.com
Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00





From daemon Wed Nov 8 02:07:23 2000



From: Gordon Couger :      gcouger-at-couger.com
Date: Tue, 7 Nov 2000 14:06:16 -0600
Subject: Re: Sound absorption curtain

Contents Retrieved from Microscopy Listserver Archives
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} From: "William Tivol" {tivol-at-wadsworth.org}
}
} Warren E Straszheim:
} }
} } You might need to expand a bit on your goal. It seems that you are
} } indicating that human speech is degrading your resolution. I find that
hard
} } to believe.
}
} Dear Warren,
} I still remember Ken Downing demonstrating that his IVEM
} image would vibrate whenever one talked at the microscope--in fact,
} at a DOE site visit, the head of the team, a Dr. Happer, seemed
} singularly unimpressed until he was shown that clapping in the
} IVEM room would cause the image to vibrate. He was apparently
} quite amused, and the site visit went well after that. The IVEM
} was known for a time as the "Happer clapper".

Noise is a problem of inches. The best solution is a corn field 5 miles
from any where in a 10 foot basment on a still night and a active noise
canceler makes it better. We put our insterments in buildings with people
and macinery. I was testing a simple load cell with a 12 bid A/D
converter. It was no trick to measure a car drive by 30 yards away a truck
100 yards a way a fellow walking by in the hall. And a load cell with a 12
bit a/d converter is not a very sensitive insterment.

There are good solutions down to 4 or 5 Hz below that you have trouble.
Floating it on an air table gets x and y but not z. floating it in liqid
work until you get a resonate situation. Active cancelation works the best
and costs the most. The things tried on this list sows that it is a
difficult problem.

You live or die by the contact with your coustomers so you lab has to be
near them. That usualy means near traffic and a lot of people. and a lot
of vibration and noise. Call your favirate noise reduction vendor and say
fixit I have this much money or go to you customers hat in hand and get
what it takes to do it right.

Good luck
Gordon
Gordon Couger gcouger-at-couger.com
Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00





From daemon Wed Nov 8 02:08:54 2000



From: Richard :      richard.langford-at-materials.oxford.ac.uk
Date: Wed, 8 Nov 2000 08:05:47 -0000
Subject: Re: 3d recon and measurement

Contents Retrieved from Microscopy Listserver Archives
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Dear Robert

The following web page gives a detailed list of software for 3-D
reconstruction.

http://biocomp.stanford.edu/3dreconstruction/index.html

Regards

Richard
--------------------------------------------------------------
Richard M Langford

Department of Materials, University of Oxford
Parks Road, Oxford, OX1 3PH, UK

Tel: +44 (0)1865 273734, Fax: +44 (0)1865 273789
email: richard.langford-at-materials.oxford.ac.uk





From daemon Wed Nov 8 03:46:37 2000



From: Tim E. Harper :      tim-at-cmp-cientifica.com
Date: Wed, 8 Nov 2000 10:43:00 +0100
Subject: Re: EDS networking

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I had the same system when I worked with ESA.
I don't remember much difficulty with SunOS 5.3. We just gave it a name & IP
address on our LAN, checked a few boxes in the set-up screen & off we went.
I don't think we even needed to call in the IT people.

If your IT people can set up a DOS box, they can set up SunOs 5.3. Should be
a 5-10 minute job, longer if they have to RTFM.

Regards

Tim


*****************************************************************
Tim E. Harper Managing Director
CMP Cientifica s.l.
Space & NanoTechnology Division
Phone +34 91 640 71 85 Fax +34 91 640 71 86
http://www.cmp-cientifica.com/

-----Original Message-----
} From: Gary Gaugler [mailto:gary-at-gaugler.com]
Sent: Wednesday, November 08, 2000 6:24 AM
To: Dave Audette
Cc: MSA listserver


Huh? What PCs? If they are all DOS-based, I can identify
with their concerns. If the PCs are running client TCP/IP,
the addition of the Sun should be trivial.

How are LAN IPs assigned? If there is a LAN NAT
server, then just assign the Sun a within-scope IP address.
If it is DHCP, just do it.

I don't see what the big deal is. Except....I'll venture
to say that the PC jockeys have no Unix experience.
That would be a good reason to keep the Sun away
from the PC LAN.

gg


At 04:36 PM 11/7/00, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America






From daemon Wed Nov 8 08:03:37 2000



From: Earl Weltmer :      earlw-at-pacbell.net
Date: Wed, 08 Nov 2000 05:33:22 -0800
Subject: change of email address

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Please change my email address from "earlw-at-pacbell.net" to
"eweltmer-at-home.com"

Thank You,

Earl Weltmer
Scanservice Corp.
(714) 573-9158




From daemon Wed Nov 8 08:03:42 2000



From: Gordon Couger :      gcouger-at-couger.com
Date: Tue, 7 Nov 2000 19:57:32 -0600
Subject: Re: EDS networking

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} From: "Gary Gaugler" {gary-at-gaugler.com}
}
} Huh? What PCs? If they are all DOS-based, I can identify
} with their concerns. If the PCs are running client TCP/IP,
} the addition of the Sun should be trivial.
}
} How are LAN IPs assigned? If there is a LAN NAT
} server, then just assign the Sun a within-scope IP address.
} If it is DHCP, just do it.
}
} I don't see what the big deal is. Except....I'll venture
} to say that the PC jockeys have no Unix experience.
} That would be a good reason to keep the Sun away
} from the PC LAN.

No it is a good reason to keep it away from the people runing the LAN. The
Lan it's self doesn't care what is hooked to it. The fool that trys to
hook it up has a lot to do wiht it. Back up everything before the come and
after they leave. So you can go back to a working state not connected to
the lan.

I can't get Unix jocks for my own projects good luck on yours. And one is
my son.

Gordon
Gordon Couger gcouger-at-couger.com
Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00





From daemon Wed Nov 8 09:18:51 2000



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Wed, 08 Nov 2000 08:56:23 -0600
Subject: Re: EDS networking

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I would echo the sentiments you have already received. It seems like your
IT folks just have no experience with UNIX. (But neither do I.) It would
take them some time to learn it, even if they are willing.

It absolutely should be a trivial matter to put a Unix box on a TCP/IP
network, and that is all you should need. If they are thinking that they
have to run Novell or some other protocol on your Unix system, that could
be a very difficult prospect. But that is not what you would want. All you
need is TCP/IP and Unix is where TCP/IP got its start.

I would suggest that you get the rules for setting up an additional PC node
on the network. Let them give you an IP number and name or tell you that
your net runs a DHCP server to hand out IP numbers. Hey, you could even
give them an old, dummy PC and say hook this up to the net and call it such
and so. Then you could have someone who does know Unix and Windows come in,
look over the setup, transfer the setting to the Unix box, and dispose of
the dummy PC.

In other words, it really should not be that tough. I bet you have someone
around that could do it. That is the way I helped another lab on campus get
their PDP-based Kevex EDS system onto the net. I had already done it with
ours.

Good luck.

Warren S.

At 04:36 PM 11/7/2000 -0800, you wrote:
} Hello listers,
}
} I operate a Noran Voyager 3.3 EDS system, which is a
} UNIX based system and operates on a Sun Sparc Station
} 5 with SunOS 5.3. The problem is the local IT
} department is reluctant to place this on the network
} which is all PC based. The IT staff feels it would be
} more cost efficient use of their time, if I found any
} product which will replace the Unix box/software and
} utilize the present hardware (detector, amplifiers,
} etc.). Anyone have any experience with this?
}
} TIA,
}
} Dave Audette
} OSRAM Sylvania
} 71 Cherry Hill Drive
} Beverly, MA 01915
} david.audette-at-sylvania.com
}
}
}
} __________________________________________________
} Do You Yahoo!?
} Thousands of Stores. Millions of Products. All in one Place.
} http://shopping.yahoo.com/




From daemon Wed Nov 8 09:22:39 2000



From: Arthur Day :      ard-at-ansto.gov.au
Date: Thu, 9 Nov 2000 02:17:19 +1100
Subject: Subject: RE: EDS networking

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Dave,

Our experience is exactly the same as Tim's. Ask your IT guys what it
is that is peculiar about your network that precludes being able to
run a unix box on it. We followed instructions provided by some user
friendly unix experts and set up our Voyagers on the network
ourselves. I've got the notes on how to do it and could send them to
you if you want. The instructions are pretty straightforward,ie the
commands to use to get to the setup screen, optional files to create
and edit if you want additional networking functions, and so on.

Maybe best if you can persuade the IT guys to assign you with a
permanent IP address. From there you shouldn't really need them for
anything else. Any other site specific info that you need should be
obtainable by looking at the network setup of any PCs (or Macs!)
running nearby.

Maybe it's a 15 minute job to do once, 5 minutes if you can avoid the damn FM.

Regards,
Arthur

}
} I had the same system when I worked with ESA.
} I don't remember much difficulty with SunOS 5.3. We just gave it a name & IP
} address on our LAN, checked a few boxes in the set-up screen & off we went.
} I don't think we even needed to call in the IT people.
}
} If your IT people can set up a DOS box, they can set up SunOs 5.3. Should be
} a 5-10 minute job, longer if they have to RTFM.
}
} Regards
}
} Tim
}
}
} *****************************************************************
} Tim E. Harper Managing Director
} CMP Cientifica s.l.
} Space & NanoTechnology Division
} Phone +34 91 640 71 85 Fax +34 91 640 71 86
} http://www.cmp-cientifica.com/
}
} -----Original Message-----
} } From: Gary Gaugler [mailto:gary-at-gaugler.com]
} Sent: Wednesday, November 08, 2000 6:24 AM
} To: Dave Audette
} Cc: MSA listserver
} Subject: Re: EDS networking
}
} At 04:36 PM 11/7/00, you wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Hello listers,
} }
} } I operate a Noran Voyager 3.3 EDS system, which is a
} } UNIX based system and operates on a Sun Sparc Station
} } 5 with SunOS 5.3. The problem is the local IT
} } department is reluctant to place this on the network
} } which is all PC based. The IT staff feels it would be
} } more cost efficient use of their time, if I found any
} } product which will replace the Unix box/software and
} } utilize the present hardware (detector, amplifiers,
} } etc.). Anyone have any experience with this?
} }
} } TIA,
} }
} } Dave Audette
} } OSRAM Sylvania
} } 71 Cherry Hill Drive
} } Beverly, MA 01915
} } david.audette-at-sylvania.com
} }
} }
} }
} } __________________________________________________
} } Do You Yahoo!?
} } Thousands of Stores. Millions of Products. All in one Place.
} } http://shopping.yahoo.com/

Arthur Day, Electron Microscope Unit Phone: 61-2-9717-3457
Ansto Materials Division Fax: 61-2-9543-7179
PMB 1, Menai (Sydney), NSW, 2234 Email: ard-at-ansto.gov.au
Australia www: http://www.ansto.gov.au/



From daemon Wed Nov 8 09:45:15 2000



From: John Foust :      jfoust-at-threedee.com
Date: Wed, 08 Nov 2000 09:33:05 -0600
Subject: Re: EDS networking

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 04:36 PM 11/7/00 -0800, Dave Audette wrote:
} I operate a Noran Voyager 3.3 EDS system, which is a
} UNIX based system and operates on a Sun Sparc Station
} 5 with SunOS 5.3. The problem is the local IT
} department is reluctant to place this on the network
} which is all PC based.

If you can find anyone there adept in Unix, they would be able
to install Samba, a networking package that will make your
box look like every other PC in the office in terms of
file sharing and printing. You can see their drives or
they can see yours.

http://us1.samba.org/samba/samba.html is the start, and
http://us5.samba.org/samba/ftp/Binary_Packages/solaris/Sparc/
has binaries or source code for your platform.

- John




From daemon Wed Nov 8 11:54:05 2000



From: Gordon Vrololjak :      gvrdolja-at-nature.Berkeley.EDU
Date: Wed, 8 Nov 2000 09:48:42 -0800 (PST)
Subject: Re: EDS networking

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Without much information on the networking setup for your lab/company, it
shouldn't be that difficult to add it to the existing windows network.
You could do it with or without your IT department.

If you have a network line, install a switch/hub, add in your Sun computer
to the network, setup its configuration, and you should be able to do
simple file transfers via ftp. You could also see if Samba is available
for your Sun OS, and then share files more easily.

More information about the network configuration that exists for your
company would be needed to give more precise suggestions.

\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
Gordon Ante Vrdoljak Electron Microscope Lab
ICQ 23243541 http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
gvrdolja-at-nature.berkeley.edu UC Berkeley
phone (510) 642-2085 Berkeley CA 94720-3330
fax (510) 643-6207 cell (510) 290-6793

On Tue, 7 Nov 2000, Dave Audette wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hello listers,
}
} I operate a Noran Voyager 3.3 EDS system, which is a
} UNIX based system and operates on a Sun Sparc Station
} 5 with SunOS 5.3. The problem is the local IT
} department is reluctant to place this on the network
} which is all PC based. The IT staff feels it would be
} more cost efficient use of their time, if I found any
} product which will replace the Unix box/software and
} utilize the present hardware (detector, amplifiers,
} etc.). Anyone have any experience with this?
}
} TIA,
}
} Dave Audette
} OSRAM Sylvania
} 71 Cherry Hill Drive
} Beverly, MA 01915
} david.audette-at-sylvania.com
}
}
}
} __________________________________________________
} Do You Yahoo!?
} Thousands of Stores. Millions of Products. All in one Place.
} http://shopping.yahoo.com/
}
}




From daemon Wed Nov 8 12:04:41 2000



From: William F. Tivol :      wft03-at-health.state.ny.us
Date: Wed, 8 Nov 2000 13:01:05 -0500
Subject: Re: Sound absorption curtain

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html







Dear Gordon,

Noise is a problem of inches.

And Hertz. Our HVEM column is very sensitive to 20 Hz, but almost
completely insensitive to frequencies a few Hz away on either side. Once you
have someone sit at the scope and watch the image as a sound generator is swept
through a range of frequencies, you will know what to be concerned about, and
then you can get the appropriate shielding.

The best solution is a corn field 5 miles
from any where in a 10 foot basment on a still night and a active noise
canceler makes it better.

If you build it, will they come? ;-)

We put our insterments in buildings with people
and macinery. I was testing a simple load cell with a 12 bid A/D
converter. It was no trick to measure a car drive by 30 yards away a truck
100 yards a way a fellow walking by in the hall. And a load cell with a 12
bit a/d converter is not a very sensitive insterment.

There are good solutions down to 4 or 5 Hz below that you have trouble.
Floating it on an air table gets x and y but not z. floating it in liqid
work until you get a resonate situation. Active cancelation works the best
and costs the most. The things tried on this list sows that it is a
difficult problem.

There is no free lunch. Floating the instrument renders it sensitive to
sound in the room, and mounting it solidly to the floor makes transmission
through the ground a problem. If the soil mechanics are such that the
frequencies transmitted to the instrument are not those to which it is
sensitive, but the sound emitted by the pumps is at the sensitive frequencies,
it can be better to bolt the instrument to the floor. As you said, an
environment completely free of vibration is best, and active cancellation is
good.
Yours,

Bill Tivol
Wadsworth Center
Albany NY
(518) 473-7399 WFT02-at-health.state.ny.us





From daemon Wed Nov 8 14:14:52 2000



From: csedax-at-alpha.arcride.edu.ar
Date: Wed, 8 Nov 2000 17:07:47 +0300
Subject: change of email address

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Please change my email address from "csedax-at-arcride.edu.ar" to
"csedax-at-ceride.gov.ar"
Thank You,

Prof. Nora Pratta
CERIDE - CONICET
Guemes 3450 - (3000)
Santa Fe, ARGENTINA



From daemon Wed Nov 8 14:36:06 2000



From: Daniel L Flatoff :      dflatoff-at-stu.madison.tec.wi.us
Date: Wed, 08 Nov 2000 20:31:38 GMT
Subject: TEM sample prep of steel

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I would like to say thanks to all for the information
provided on preparing TEM cross sections of DLC thin films
on steel. Also I would like to note that I do have access
a FIB tool at Madison Area Technical College, so anyone who
has any info on specialized techniques for the TEM prep of
DLC thin films using the FIB would greatly be appreciated.
Again, thanks to all


Daniel L. Flatoff
student
Madison Area Technical College






From daemon Wed Nov 8 15:05:56 2000



From: Jesse Rodrigues :      Jesse_Rodrigues-at-kopin.com
Date: Wed, 8 Nov 2000 16:01:39 -0500
Subject: ISO calibration of an SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Hi,

Does anyone have any experience with instituting ISO compliance of SEM's?
How do you keep your SEM calibrated and how often are calibrations
performed? What type of support does your mfg. offer?

Any information would be helpful.

Thank you,

Jesse Rodrigues
Kopin Corporation
695 Myles Standish Blvd.
Taunton, MA 02780





From daemon Wed Nov 8 15:14:46 2000



From: Thearith H. Ung :      tung-at-qdots.com
Date: Wed, 8 Nov 2000 13:08:38 -0800
Subject: TEM CCD Camera/EDS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hi all,

Thank you to those who replied and let me know that their cameras and EDS
systems are available for sale. I forgot to ask also what are some of the
best vendors that sell CCD cameras and EDS systems? This information will be
valuable in helping me to apply for grants to purchase these instruments.

Thank you in advance.

Regards,
Thearith
_________________________

Thearith Ung, Ph.D.
Quantum Dot Corporation
26136 Research Road
Hayward CA 94545
Tel: +1-510-887-8775 (x4125)
Fax:+1-510-783-9729
www.qdots.com




From daemon Wed Nov 8 15:35:32 2000



From: Anthony Garratt-Reed :      tonygr-at-mit.edu
Date: Wed, 08 Nov 2000 16:30:14 -0500
Subject: Re: EDS networking

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 04:36 PM 11/7/2000 -0800, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

} From Dave's posting, we can't tell. Before he starts any battles with his
IT department, he needs to learn (by his own research or with the help of
an advocate) what the reasons are for the IT advice. Maybe he already
knows this and didn't include it in his message (but I suspect not, for
why would he have posted his message). Without this knowledge, though, the
various responses (excepting Scott's) that have been made are not really
very helpful.

I know I'm on a soapbox here, and in any forum like this, a major degree
of *caveat emptor* has to apply. It is certainly true that, if the
OSRAM/Sylvania network is TCP/IP, then the UNIX box is easily networked.
If not, though, then Dave will have been misguided, rather than helped, by
the responses he has received.

Tony.



} Hello listers,
}
} I operate a Noran Voyager 3.3 EDS system, which is a
} UNIX based system and operates on a Sun Sparc Station
} 5 with SunOS 5.3. The problem is the local IT
} department is reluctant to place this on the network
} which is all PC based. The IT staff feels it would be
} more cost efficient use of their time, if I found any
} product which will replace the Unix box/software and
} utilize the present hardware (detector, amplifiers,
} etc.). Anyone have any experience with this?
}
} TIA,
}
} Dave Audette
} OSRAM Sylvania
} 71 Cherry Hill Drive
} Beverly, MA 01915
} david.audette-at-sylvania.com
}
}
}
} __________________________________________________
} Do You Yahoo!?
} Thousands of Stores. Millions of Products. All in one Place.
} http://shopping.yahoo.com/
}

** Anthony J. Garratt-Reed
** MIT Room # 13-1027
** 77 Massachusetts Avenue
** Cambridge, MA 02139-4307
** USA
**
** Phone: (+) 1-617-253-4622
** Fax: (+) 1-617-258-6478
**





From daemon Wed Nov 8 16:47:32 2000



From: Walck, Scott D. :      walck-at-ppg.com
Date: Wed, 8 Nov 2000 17:40:52 -0500
Subject: RE: ISO calibration of an SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We are QS9000 certified which is the automotive equivalent to ISO.
Basically, it really is up to you how often you want to do it. We have some
instruments set up for twice a year and some on an annual basis. Basically,
the ISO is a paper work tracking system that insures that you do what you
say you do in your SOP's or in our case, SLP's (System Level Procedures).
You decide what you want to do and stick to it. You write the
specifications that you need to follow and document them. A suggestion,
don't write them too stringent. You can use the ISO process to insure that
your instrumentation is maintained properly. For example if you write a
specification that your EDS detector should have a particular energy
resolution for an element and if it is out, that the detector is repaired,
then your company has no options, it must have it repaired or have it taken
out of service. However, you should be careful in writing your procedures
if you don't want it out of service. For example, you don't want the
normal resolution degradation in performance with lifetime of a detector
shutting you down.

We have the manufacturer's or our 3rd party's service engineers verify the
calibration as part of the annual or semi-annual PM visit on our service
contracts on all our scanning instruments. On our TEM, I just do the annual
calibration verification myself and record it. If I do anything that
requires a new calibration before the year is up, I just put the new
calibration data in the records book. It doesn't hurt to do it more often.
It is a real pain if you find out that it was out of calibration and you
were sending data to customers. Then you have to contact them all and tell
them that their results were done while it was not in calibration.


-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)



} -----Original Message-----
} From: Jesse Rodrigues [mailto:Jesse_Rodrigues-at-kopin.com]
} Sent: Wednesday, November 08, 2000 4:02 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: ISO calibration of an SEM
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListse} rver/FAQ.html
}
}
} --------------------------------------------------------------
} ---------.
}
}
}
}
} Hi,
}
} Does anyone have any experience with instituting ISO
} compliance of SEM's?
} How do you keep your SEM calibrated and how often are calibrations
} performed? What type of support does your mfg. offer?
}
} Any information would be helpful.
}
} Thank you,
}
} Jesse Rodrigues
} Kopin Corporation
} 695 Myles Standish Blvd.
} Taunton, MA 02780
}
}
}



From daemon Wed Nov 8 18:04:10 2000



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Wed, 08 Nov 2000 15:55:55 -0800
Subject: Re: Sound absorption curtain

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers,
A test I have heard of for the acoustic resonence frequency of your TEM
sample holder and stage is the "whistle test". While looking at a sample
with good high res. detail through the binocular eyepieces, mag. 100,000 to
200,000 X, whistle starting low and going up. You should see a frequency in
there where the sample vibrates. Never move or speak while taking a high
res. photo.
At 01:01 PM 11/8/00 -0500, you wrote:
} Dear Gordon,
}
} Noise is a problem of inches.
}
} And Hertz. Our HVEM column is very sensitive to 20 Hz, but almost
} completely insensitive to frequencies a few Hz away on either side. Once you
} have someone sit at the scope and watch the image as a sound generator is swept
} through a range of frequencies, you will know what to be concerned about, and
} then you can get the appropriate shielding.
}
} The best solution is a corn field 5 miles
} from any where in a 10 foot basment on a still night and a active noise
} canceler makes it better.
}
} If you build it, will they come? ;-)
}
} We put our insterments in buildings with people
} and macinery. I was testing a simple load cell with a 12 bid A/D
} converter. It was no trick to measure a car drive by 30 yards away a truck
} 100 yards a way a fellow walking by in the hall. And a load cell with a 12
} bit a/d converter is not a very sensitive insterment.
}
} There are good solutions down to 4 or 5 Hz below that you have trouble.
} Floating it on an air table gets x and y but not z. floating it in liqid
} work until you get a resonate situation. Active cancelation works the best
} and costs the most. The things tried on this list sows that it is a
} difficult problem.
}
} There is no free lunch. Floating the instrument renders it sensitive to
} sound in the room, and mounting it solidly to the floor makes transmission
} through the ground a problem. If the soil mechanics are such that the
} frequencies transmitted to the instrument are not those to which it is
} sensitive, but the sound emitted by the pumps is at the sensitive frequencies,
} it can be better to bolt the instrument to the floor. As you said, an
} environment completely free of vibration is best, and active cancellation is
} good.
} Yours,
}
} Bill Tivol
} Wadsworth Center
} Albany NY
} (518) 473-7399 WFT02-at-health.state.ny.us
}
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca




From daemon Wed Nov 8 18:07:00 2000



From: Eric Steel :      eric.steel-at-nist.gov
Date: Wed, 08 Nov 2000 19:03:35 -0500
Subject: Post Doctoral Positions

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{html}
The National Institute of Standards & Technology (NIST) has many Post
Doctoral Positions open. These are offered competitively through the
National Research Council (NRC).  Within
microscopy/microanalysis/surface analysis research areas we have many
possible openings described at the NRC web sites listed below.  The
Microanalysis Research and Analytical Microscopy Groups at NIST have
about twenty full time scientists specializing in the measurement methods
in our extensive facilities including SEMs (FEG, EPMA, Environmental,=85),
AEMs (FEG/LaB6 TEM/STEM/EDS/EELS), Auger probes, SIMS/TOF-SIMS,
MicroXRD/XRF, MicroRaman/IR, Synchrotron beam-line with grazing incidence
XPS, etc.   We research new and improved measurement methods
and we apply these methods to challenging analytical problems in
semiconductor and optoelectronic technology, materials science,
environmental and earth science, etc. {br}
{br}
If you have research ideas that would be related to the analytical
approaches below and are looking for a Post Doc opportunity, please
contact me. {br}
{br}
The NIST/NRC program offers a two-year Post Doc at an annual salary of
$50,000 with an additional $5,500 for research expenses. The applications
are {b} due to the NRC by Jan 15, 2001 {/b} . This includes a brief proposal
and several recommendations. {br}
{br}
{b} A candidate must be a U.S. citizen and start work (with their PhD in
hand) at NIST between July 1, 2001 and Feb 1, 2002. {/b} So, this is the
perfect opportunity for those that are graduating this spring through
next fall and others that have received their degree within the last five
years. Please note that NIST/NRC only competes Post Doc positions once a
year, unlike some other institutions. {br}
{br}
{b} General NIST-NRC Post Doc Information {br}
{/b} {font size=3D1 color=3D"#0000FF"} {u} {a=
href=3D"http://www4.nas.edu/osep/rap.nsf/vwLabInformation/03AF8E43C706150B8=
52567FB004AB8E8?OpenDocument"=
eudora=3D"autourl"} http://www4.nas.edu/osep/rap.nsf/vwLabInformation/03AF8E=
43C706150B852567FB004AB8E8?OpenDocument {/a} {br}
{br}
{/font} {/u} Specific areas of interest: {br}
{br}
{b} Chemical Imaging {br}
{/b} {font size=3D1 color=3D"#0000FF"} {u} {a=
href=3D"http://www4.nas.edu/osep/rap.nsf/ByTitle/5273986D8FFE74B18525691F00=
60BD9F?OpenDocument&50~NIST"=
eudora=3D"autourl"} http://www4.nas.edu/osep/rap.nsf/ByTitle/5273986D8FFE74B=
18525691F0060BD9F?OpenDocument&50~NIST {/a} {br}
{br}
{/font} {/u} {b} Field Emission Analytical Electron Microscopy/Nanoscale
Compositional Mapping {br}
{/b} {font size=3D1 color=3D"#0000FF"} {u} {a=
href=3D"http://www4.nas.edu/osep/rap.nsf/ByTitle/65DE73654E7869028525691F00=
60A463?OpenDocument&50~NIST"=
eudora=3D"autourl"} http://www4.nas.edu/osep/rap.nsf/ByTitle/65DE73654E78690=
28525691F0060A463?OpenDocument&50~NIST {/a} {br}
{br}
{/font} {/u} {b} Electron-Probe Microanalysis/Scanning Electron
Microscopy {br}
{/b} {font size=3D1 color=3D"#0000FF"} {u} {a=
href=3D"http://www4.nas.edu/osep/rap.nsf/ByTitle/F704FABB051A2EE48525691F00=
60A4B5?OpenDocument&50~NIST"=
eudora=3D"autourl"} http://www4.nas.edu/osep/rap.nsf/ByTitle/F704FABB051A2EE=
48525691F0060A4B5?OpenDocument&50~NIST {/a} {br}
{br}
{/font} {/u} {b} Microchemistry in the Environmental Scanning Electron
Microscope {br}
{/b} {font size=3D1 color=3D"#0000FF"} {u} {a=
href=3D"http://www4.nas.edu/osep/rap.nsf/ByTitle/944FC47A85E417348525691F00=
60C301?OpenDocument&50~NIST"=
eudora=3D"autourl"} http://www4.nas.edu/osep/rap.nsf/ByTitle/944FC47A85E4173=
48525691F0060C301?OpenDocument&50~NIST {/a} {br}
{br}
{/font} {/u} {b} Microbeam Mass Spectrometry {br}
{/b} {font size=3D1 color=3D"#0000FF"} {u} {a=
href=3D"http://www4.nas.edu/osep/rap.nsf/ByTitle/623A17BC37343CDC8525691F00=
60A44D?OpenDocument&50~NIST"=
eudora=3D"autourl"} http://www4.nas.edu/osep/rap.nsf/ByTitle/623A17BC37343CD=
C8525691F0060A44D?OpenDocument&50~NIST {/a} {br}
{br}
{/font} {/u} {b} Nanoscale Crystallographic Analysis and Compound
Identification by Diffraction Methods {br}
{/b} {font size=3D1 color=3D"#0000FF"} {u} {a=
href=3D"http://www4.nas.edu/osep/rap.nsf/ByTitle/39166A04610797758525691F00=
60C316?OpenDocument&50~NIST"=
eudora=3D"autourl"} http://www4.nas.edu/osep/rap.nsf/ByTitle/39166A046107977=
58525691F0060C316?OpenDocument&50~NIST {/a} {br}
{br}
{/font} {/u} {b} Optical Probe Microanalysis (Raman, IR Microprobes, and
NSOM) {br}
{/b} {font size=3D1 color=3D"#0000FF"} {u} {a=
href=3D"http://www4.nas.edu/osep/rap.nsf/ByTitle/78E3849178AC6C718525691F00=
60A43A?OpenDocument&50~NIST"=
eudora=3D"autourl"} http://www4.nas.edu/osep/rap.nsf/ByTitle/78E3849178AC6C7=
18525691F0060A43A?OpenDocument&50~NIST {/a} {br}
{br}
{/font} {/u} {b} Submicroscopic Chemical and Physical Characterization of
Materials and Particles {br}
{/b} {font size=3D1 color=3D"#0000FF"} {u} {a=
href=3D"http://www4.nas.edu/osep/rap.nsf/ByTitle/9C8BD7F79A5AAACE8525691F00=
60A4A1?OpenDocument&50~NIST"=
eudora=3D"autourl"} http://www4.nas.edu/osep/rap.nsf/ByTitle/9C8BD7F79A5AAAC=
E8525691F0060A4A1?OpenDocument&50~NIST {/a} {br}
{br}
{/font} {/u} {b} Surface Microanalysis by Combined Auger Electron and X-Ray
Emission Spectroscopies {br}
{/b} {font size=3D1 color=3D"#0000FF"} {u} {a=
href=3D"http://www4.nas.edu/osep/rap.nsf/ByTitle/9ACCD2318EE9230F8525691F00=
60B7E6?OpenDocument&50~NIST"=
eudora=3D"autourl"} http://www4.nas.edu/osep/rap.nsf/ByTitle/9ACCD2318EE9230=
F8525691F0060B7E6?OpenDocument&50~NIST {/a} {br}
{br}
{/font} {/u} {b} X-Ray Investigations of Thin Films, Surfaces, and
Interfaces {br}
{/b} {font size=3D1 color=3D"#0000FF"} {u} {a=
href=3D"http://www4.nas.edu/osep/rap.nsf/ByTitle/362CD137E15600568525691F00=
60A4D5?OpenDocument&50~NIST"=
eudora=3D"autourl"} http://www4.nas.edu/osep/rap.nsf/ByTitle/362CD137E156005=
68525691F0060A4D5?OpenDocument&50~NIST {/a} {br}
{br}
{/font} {/u} {b} Instrument Performance Standards for Raman
Spectroscopy {br}
{/b} {font size=3D1 color=3D"#0000FF"} {u} {a=
href=3D"http://www4.nas.edu/osep/rap.nsf/ByTitle/5E60A8DDDF3EB7DE8525691F00=
60BF12?OpenDocument&50~NIST"=
eudora=3D"autourl"} http://www4.nas.edu/osep/rap.nsf/ByTitle/5E60A8DDDF3EB7D=
E8525691F0060BF12?OpenDocument&50~NIST {/a} {br}
{br}
{/font} {/u} {b} Quantitative Surface Analysis by Electron=20
Spectroscopy {br}
{/b} {font size=3D1 color=3D"#0000FF"} {u} {a=
href=3D"http://www4.nas.edu/osep/rap.nsf/ByTitle/D0188B23B4CC87B78525691F00=
60BD89?OpenDocument&50~NIST"=
eudora=3D"autourl"} http://www4.nas.edu/osep/rap.nsf/ByTitle/D0188B23B4CC87B=
78525691F0060BD89?OpenDocument&50~NIST {/a} {br}
{br}
{/font} {/u} {br}
{br}
{div} Eric B. Steel,
Leader           &nbs=
p;  
{x-tab}      {/x-tab} e-mail:=20
eric.steel-at-nist.gov {/div}
{div} Microanalysis Research
Group       
{x-tab}      {/x-tab} Office: 301-975-3902 {/div}
{div} N.I.S.T.          &nb=
sp;            &=
nbsp;   
{x-tab}      {/x-tab} {x-tab}      =
    {/x-tab} FAX: 
301-417-1321 {/div}
{div} 100 Bureau Drive, Stop 8371 {/div}
{div} Gaithersburg, MD 20899-8371 {/div}
{a href=3D"http://www.nist.gov/cstl/div837/837.02/"=
EUDORA=3DAUTOURL} http://www.nist.gov/cstl/div837/837.02/ {/a}
{/html}




From daemon Wed Nov 8 19:20:51 2000



From: Victor Sidorenko :      antron-at-space.ru
Date: Thu, 9 Nov 2000 03:58:31 +0300
Subject: Re: Leo S360 4QBSD

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Dear Robert!

I seem you went to reflective mode of work. Sometimes it happens when
charge does not flow from sample for some reasons. In this case you
can see in backscattered electrons the image of the detector at
polepiece (and some halves or quadrants will change contrast depending
on what mode you choose), and in secondary electrons - all specimen
chamber from inside.
This effect can be used for example for testing of BSE signal path.

Advises:
1. Test the electrical path between the sample and microscope case (if
you have absorbed current amplifier or meter the resistance may be
large).
2. Check this effect on metal test sample.
2. Sputter non-metal sample by conductive film.
3. Work in low vacuum mode if you have it.

Hope it helps.
Regards

Victor Sidorenko, ANTRON Co. Ltd., Moscow, Russia.


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From daemon Wed Nov 8 19:25:44 2000



From: Gary Gaugler :      gary-at-gaugler.com
Date: Wed, 08 Nov 2000 17:18:18 -0800
Subject: Re: ISO calibration of an SEM

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That is a good question and I'm sure, one which will generate
a high degree of variability of responses.

My (un-named) FESEM company does calibrations based on
when the preventive maintenance (PM) routines are performed.
These are supposed to be done at six month intervals. Actually,
they get done only if I have a hard failure of the system and
then when that problem is fixed, a PM is done....if there is time
to do it.

In reality, I can do the key measurements myself to ensure
that the SEM is in spec. Gun brightness, image symmetry, mag
accuracy, etc.

I suppose that it depends on how much you want or need the
maker to do the calibration versus what you can do or are
allowed to do yourself.

gg



At 01:01 PM 11/8/00, you wrote:

} Hi,
}
} Does anyone have any experience with instituting ISO compliance of SEM's?
} How do you keep your SEM calibrated and how often are calibrations
} performed? What type of support does your mfg. offer?
}
} Any information would be helpful.
}
} Thank you,
}
} Jesse Rodrigues
} Kopin Corporation
} 695 Myles Standish Blvd.
} Taunton, MA 02780




From daemon Wed Nov 8 20:04:37 2000



From: Gordon Couger :      gcouger-at-couger.com
Date: Wed, 8 Nov 2000 08:02:20 -0600
Subject: Re: EDS networking

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from: "Anthony Garratt-Reed" {tonygr-at-mit.edu}
} Despite what many respondants to this message have said/implied, it is
} entirely possible that Dave's IT department does know what it is talking
} about. A Department/Plant network need not be implemented using TCP/IP,
} and if it is all PC-based, and doesn't require routing, then NetBEUI
might
} be a sensible choice. IPX/SPX (Novell) networking, or even Lantastic or
} another proprietary system may alternatively be in use.

Then let them build a gateway to put the Unix box on what ever they have.
Gordon
Gordon Couger gcouger-at-couger.com
Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00





From daemon Wed Nov 8 21:47:43 2000



From: Gordon Couger :      gcouger-at-couger.com
Date: Wed, 8 Nov 2000 08:44:04 -0600
Subject: Re: Sound absorption curtain

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} From: "William F. Tivol" {wft03-at-health.state.ny.us}
,
}
Dear Bill,

} Noise is a problem of inches.

How true and the solution is often found by Edisonian iteration.
}
} And Hertz. Our HVEM column is very sensitive to 20 Hz, but almost
} completely insensitive to frequencies a few Hz away on either side.
Once you
} have someone sit at the scope and watch the image as a sound generator
is swept
} through a range of frequencies, you will know what to be concerned
about, and
} then you can get the appropriate shielding.
========
Have you tried to find what is resonate at 20 Hz and do somting to break
it up. That is high enough you might have a small chance of doing
something about it.
}
} The best solution is a corn field 5 miles
} from any where in a 10 foot basement on a still night and a active noise
} canceler makes it better.
}
} If you build it, will they come? ;-)
}
We really do a lot of radio frequency noise testing in remote fields.
Motorola has a 3 million dollar RF test chamber to test and certify some
wireless devices for noise output on the receiving frequency. The company
that owns the net work has a particular cornfield in Iowa they use for the
same tests for considerably less money. John Deere tests the computers on
their tractors for noise output and immunity by driving them out in the
middle of a field using battery operated test instruments.

I built some sensors used on Ag machinery. It is the quietest environment
I have ever seen The only noise was from the alternator on the diesel
engine and the computers. NO 60 or 120 CPS noise at all.

Gordon
Gordon Couger gcouger-at-couger.com
Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00






From daemon Thu Nov 9 00:14:10 2000



From: Gary Gaugler :      gary-at-gaugler.com
Date: Wed, 08 Nov 2000 22:04:48 -0800
Subject: Re: EDS networking

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At 06:02 AM 11/8/00, you wrote:

} from: "Anthony Garratt-Reed" {tonygr-at-mit.edu}
} Despite what many respondants to this message have said/implied, it is
} entirely possible that Dave's IT department does know what it is talking
} about. A Department/Plant network need not be implemented using TCP/IP,
} and if it is all PC-based, and doesn't require routing, then NetBEUI
} might be a sensible choice. IPX/SPX (Novell) networking, or even
} Lantastic or
} another proprietary system may alternatively be in use.
}
} Then let them build a gateway to put the Unix box on what ever they have.
} Gordon
} Gordon Couger gcouger-at-couger.com
} Stillwater, OK www.couger.com/gcouger
} 405 624-2855 GMT -6:00

I guess that I just don't see what the big deal is here. Get a static
IP, agreed-upon host name and set the Unix box up.

If the system is on a LAN, I don't see why routing is an issue.
That will/should be transparent as long as the main router is
set at the default gateway. If a bridge is involved, I can see
that things might be more complicated--but not insurmountable.

Unix/Linux to PCs and Macs are done all the time. No big deal.
My own LAN is exactly like this. Mac G4/400, 3 P-3/800,
SGI O2, SGI Indigo 2, Sun Ultra 5, P2-400 Linux. All on
10BaseT running TCP/IP. PCs use Microsoft TCP/IP
client. Mac runs DAVE. SGI runs IRIX 6.5. Sun runs
SunOS/Solaris.

I simply do not see the big deal here. The key is to get a
static IP on the branch LAN, know the gateway IP, and
provide DNS IPs. That ought to do it.

gg




From daemon Thu Nov 9 04:16:14 2000



From: Robert McDonald :      robert-at-starav.geology.gla.ac.uk
Date: Thu, 09 Nov 2000 10:25:28 +0000
Subject: Thanks to all Re; 4QBSD

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Just a quick "Thanks" to all those who took the time to answer my 4QBSD
problem without laughing at my limited knowledge :-)

Robert




From daemon Thu Nov 9 04:57:42 2000



From: David Cockayne :      david.cockayne-at-materials.oxford.ac.uk
Date: Thu, 9 Nov 2000 10:47:58 -0000
Subject: Fw: Monte Carlo simulation

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I am in need of a Monte Carlo electron scattering programme (with or without
EDS) for SEM scattering simulations. Is there one for downloading for a PC,
freeware or otherwise?

David Cockayne
Department of Materials
University of Oxford
Parks Road
Oxford OX1 3PH
England

tele +44 01865 273654
fax +44 01865 283329

email david.cockayne-at-materials.oxford.ac.uk





From daemon Thu Nov 9 07:40:44 2000



From: David Cockayne :      david.cockayne-at-materials.oxford.ac.uk
Date: Thu, 9 Nov 2000 07:35:08 -0600
Subject: Monte Carlo simulation

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I am in need of a Monte Carlo electron scattering programme (with or without
EDS). Is there one for downloading for a PC, freeware or otherwise?




Professor David Cockayne FRS
Department of Materials
University of Oxford
Parks Road
Oxford OX1 3PH
England

tele +44 01865 273654
fax +44 01865 283329

email david.cockayne-at-materials.oxford.ac.uk





From daemon Thu Nov 9 07:46:51 2000



From: Ladd Research :      sales-at-laddresearch.com
Date: Thu, 09 Nov 2000 08:42:17 -0500
Subject: Re: Spurr will be discontinued?

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Maria,

Union Carbide is the manufacturer of ERL 4206, which is a component of
of the Spurr Kit. They have discontinued the production of ERL 4206, but
they do supply ERL 4221 which does not have the low viscosity of the ERL
4206.
We continue to have ERL 4206 and Spurr Kits in stock and we are
confident that we will have an ideal substitute for this product very
shortly.

John Arnott
We continue
--

LADD RESEARCH
131 Dorset Lane
Williston, VT 05495 USA

web site http://www.laddresearch.com

TEL 1-800-451-3406 (US) or 1-802-878-6711 (anywhere)
FAX 1-802-878-8074
e-mail sales-at-laddresearch.com

Quality Since 1955


Mendes, Maria wrote:

} Hello I would like to subscribe to your service, please let me know if there
} is any service charge.
} Please pass on my problem to your subscribers.
} I am a research lab that does a lot of hard tissue processing, I as well as
} our EM departments have been using SPURR as our plastic of choice. I have
} been recently told by my supplier (Marivac) that in six months one of the
} components of SPURR will be discontinued. The component is ERL
} (Vinylcyclohexene Dioxide). I would like to know if others are using SPURR
} and if so have they encountered the same problem. Please forward your
} information to mmendes-at-mtsinai.on.ca.
} Thank-you in advance for any help offered.
}
} Maria


--

LADD RESEARCH
131 Dorset Lane
Williston, VT 05495 USA

web site http://www.laddresearch.com

TEL 1-800-451-3406 (US) or 1-802-878-6711 (anywhere)
FAX 1-802-878-8074
e-mail sales-at-laddresearch.com

Quality Since 1955



From daemon Thu Nov 9 07:57:36 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Thu, 9 Nov 2000 07:53:01 -0600
Subject: Re: Fw: Monte Carlo simulation

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David

There are some OLD programs in the ANL Software library archives....

ftp://www.amc.anl.gov/AMC-3/ANLSoftwareLibrary/


for example here are some of David Joy's early versions of MC programs

ftp://www.amc.anl.gov/AMC-3/ANLSoftwareLibrary/3-MASLIB/MONTCARL/
ftp://www.amc.anl.gov/AMC-3/ANLSoftwareLibrary/2-EMMPDL/Xeds/Dcjaemmc/

but I would suggest that you also go to the CASINO WWW site at the
University of Sherbrooke

http://www.gme.usherb.ca/casino


Cheers....

Nestor
Your Friendly Neighborhood SysOp.




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From daemon Thu Nov 9 09:07:18 2000



From: =?utf-8?B?V2FsY2ssIFNjb3R0IEQu?= :      walck-at-ppg.com
Date: Thu, 9 Nov 2000 09:56:14 -0500
Subject: =?utf-8?B?UkU6IFRFTSBzYW1wbGUgcHJlcCBvZiBzdGVlbA==?=

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I just visited with Lucille Giannuzzi at Univ. Central Florida where she
taught me a few things about FIB. I did ask her about carbon. She told me
that they have a water injector to help with carbon films such as resists on
semiconductors. Based on my experiences with DLC films, I suspect that you
will need to use that to get reactive etching going to cut through the DLC.
You will probably need to cut the samples normally, but you will also will
need to watch out for channeling affects. Again, I learned more about this
from Lucille.

I'm not the expert here. You might consider contacting one.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)


"The opinions expressed are those of Scott D. Walck and not of PPG
Industries, Inc. nor of any PPG-associated companies."
--




} -----Original Message-----
} From: Daniel L Flatoff [mailto:dflatoff-at-stu.madison.tec.wi.us]
} Sent: Wednesday, November 08, 2000 3:32 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: TEM sample prep of steel
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
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}
}
} --------------------------------------------------------------
} ---------.
}
}
} I would like to say thanks to all for the information
} provided on preparing TEM cross sections of DLC thin films
} on steel. Also I would like to note that I do have access
} a FIB tool at Madison Area Technical College, so anyone who
} has any info on specialized techniques for the TEM prep of
} DLC thin films using the FIB would greatly be appreciated.
} Again, thanks to all
}
}
} Daniel L. Flatoff
} student
} Madison Area Technical College
}
}
}
}



From daemon Thu Nov 9 09:46:28 2000



From: John Foust :      jfoust-at-threedee.com
Date: Thu, 09 Nov 2000 09:36:07 -0600
Subject: Another digital camera twist

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The other day while on a consulting job that entailed
photographing a set of ceramic tiles in a range of colors,
I discovered an interesting quirk of at least this
Olympus C-2500L digital camera. I suspect that other
consumer/prosumer cameras would suffer the same fate.

There were four tiles ranging from, say, very light purple
to dark purple. We'd put them on a black velvet background.
We had tremendous trouble getting a accurate color. The two
light tiles would be too bright and washed-out, and the two
dark tiles would be too dark and not quite right. For that
matter, the black wasn't black.

I recalled an article I'd read about the custom chips
in this Olympus camera, and how it was much more an active
system than just a CCD pixel bucket. It tries to make a
nice picture based on what it saw.

My hypothesis was to add other objects to the scene - sure
enough, after adding a few other colorful objects at the
margins of the frame, the appearance of the tiles changed
dramatically for the better. Now they were a range.
It's as if it needs a sacrificial white and black and
other colors in order to produce a decent image.

Certainly this has implications for microscope use of
today's digital cameras. I don't see a way to turn
off this feature, at least on this camera.

- John




From daemon Thu Nov 9 10:13:09 2000



From: Tobias Baskin :      BaskinT-at-missouri.edu
Date: Thu, 9 Nov 2000 10:03:34 -0600
Subject: digitizing tablet software MAC

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greetings,
Does anyone know of software that will run a digitizing
tablet for the macintosh? I am thinking of something like "sigmascan"
which runs on the PC. We just need to measure lengths and angles,
nothing to fancy.

Thanks for the help.
Tobias Baskin
--
_ ____ __ ____ Tobias I. Baskin
/ \ / / \ / \ \ 109 Tucker Hall
/ / / / \ \ \ Biological Sciences
/_ / __ /__ \ \ \__ University of Missouri
/ / / \ \ \ Columbia, MO USA
/ / / \ \ \ 65211-7400
/ / ___ / \ \__/ \ ____ voice: 573-882-0173
fax: 573-882-0123



From daemon Thu Nov 9 11:23:58 2000



From: David Cockayne :      david.cockayne-at-materials.oxford.ac.uk
Date: Thu, 9 Nov 2000 17:11:08 -0000
Subject: Monte Carlo

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


To all those who sent me advice re a source of Monte Carlo simulations MANY
THANKS.

There were so many, PLEASE, NO MORE

And if I don't thank personally all those who replied, please accept my
thanks here.



David Cockayne




From daemon Thu Nov 9 12:40:43 2000



From: Jennifer Palmer :      jpalmer-at-cvmbs.colostate.edu
Date: Thu, 9 Nov 2000 11:33:32 -0700 (Mountain Standard Time)
Subject: t-BGE dehydration

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does anyone have references and recommendations(or caveats)for
dehydration of tissue using tBGE? We routinely prep (testis and
ovary) with PO through Epon812 and want something less toxic that
still gives good ultrastructure.

====================================
Jennifer Palmer
ARBL
Colorado State Univ
Ft. Collins, CO 80523-1683 , USA
voice:(970)491-1770
fax:(970)491-3557

jpalmer-at-cvmbs.colostate.edu
====================================




From daemon Thu Nov 9 12:43:55 2000



From: Nestor J. Zaluzec :      zaluzec-at-aaem.amc.anl.gov
Date: Thu, 9 Nov 2000 12:40:35 -0600
Subject: Re: digitizing tablet software MAC

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Tobias

Why not just simply digitize the image and then
use NIH Image to measure distances and angles, this
will produce a nicely documented measurement as
you'll have the image, and all the numerical values
stored.

I do this all the time. Of course, it presumes
that you have a digitized image to start with.

Nestor
Your Friendly Neighborhood SysOp



} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


==================================================================
Dr. Nestor J. Zaluzec
Materials Science Division
Building 212
Argonne National Lab
9700 S. Cass Ave
Argonne, Illinois 60439 USA
Tel: 630-252-7901, Fax: 630-252-4798
Email: Zaluzec-at-aaem.amc.anl.gov
==================================================================
TPMLab: http://tpm.amc.anl.gov
MMSite: http://www.amc.anl.gov
==================================================================

The box said "This program requires Win 95/98/NT or better..."
So I bought a G3 Mac !

==================================================================





From daemon Thu Nov 9 13:17:10 2000



From: Debby Sherman :      sherman-at-btny.purdue.edu
Date: 09 Nov 2000 16:43:30 -0500
Subject: TEM calibration sample

Contents Retrieved from Microscopy Listserver Archives
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Dear List,

Does anyone know a good reference for the origin of the top-bottom effect? TIA.
Yours,

Bill Tivol
Wadsworth Center
Albany NY
(518) 473-7399 WFT02-at-health.state.ny.us




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The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Hi all,
I am looking for a sample to calibrate magnifications of a TEM from 50,000X up. I have used catalase in the past. However the periodicity of catalase is ~8.7nm. That is too large for my present purposes as I am looking at structures in the 2-4nm range and want to reduce the margin of error in the measurements as much as possible. I would also like a sample that is easy to used by inexperienced microscopists in the hopes of encouraging more users to calibrate instrument magnification on a regular basis. It also needs to be relatively in expensive so the loss of a grid is not a catastrophy.
I would like a standard that would have ~1.0nm lattice. I thought I had found the perfect sample in copper phthalocyanine which has a spacing in this range. Unfortunately the only source of a grid with this crystal has discontinued carrying it. I am back to square one. My options are to prepare my own grid but need advise on how to crystallize the copper phthalocyanine (available from Sigma in powder form) or find another crystalline lattice in this size range.
I would appreciate suggestions for alternative crystals and/or methods for making the copper phthalocyanine crystals to use for this purpose.
Thanks in advance.
Debby

Debby Sherman Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: sherman-at-btny.purdue.edu
1057 Whistler Building
West Lafayette, IN 47907-1057




From daemon Fri Nov 10 02:11:40 2000



From: Ron Doole :      ron.doole-at-materials.oxford.ac.uk
Date: Fri, 10 Nov 2000 08:10:12 +0000 (GMT Standard Time)
Subject: Re: TEM calibration sample

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Hi Debbie,

What about crocidolite 0.9nm (020) and 0.45nm (021)
spacings. It's a long time since I used it but it is still
in catalogues and cheap (Agar Scientific at £7 sterling
each and probably others).

Good luck,
Ron

On 09 Nov 2000 16:43:30 -0500 Debby Sherman
{sherman-at-btny.purdue.edu} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi all,
} I am looking for a sample to calibrate magnifications of a TEM from 50,000X up. I have used catalase in the past. However the periodicity of catalase is ~8.7nm. That is too large for my present purposes as I am looking at structures in the 2-4nm range and want to reduce the margin of error in the measurements as much as possible. I would also like a sample that is easy to used by inexperienced microscopists in the hopes of encouraging more users to calibrate instrument magnification on a regular basis. It also needs to be relatively in expensive so the loss of a grid is not a catastrophy.
} I would like a standard that would have ~1.0nm lattice. I thought I had found the perfect sample in copper phthalocyanine which has a spacing in this range. Unfortunately the only source of a grid with this crystal has discontinued carrying it. I am back to square one. My options are to prepare my own grid but need advise on how to crystallize the copper phthalocyanine (available from Sigma in powder form) or find another crystalline lattice in this size range.
} I would appreciate suggestions for alternative crystals and/or methods for making the copper phthalocyanine crystals to use for this purpose.
} Thanks in advance.
} Debby
}
} Debby Sherman Phone: 765-494-6666
} Life Science Microscopy Facility FAX: 765-494-5896
} Purdue University E-mail: sherman-at-btny.purdue.edu
} 1057 Whistler Building
} West Lafayette, IN 47907-1057
}
}

----------------------
Mr. R.C. Doole
Department of Materials,
University of Oxford.
Parks Road, Oxford. OX1 3PH. UK.
Phone +44 (0) 1865 273701
Fax +44 (0) 1865 283333
ron.doole-at-materials.ox.ac.uk




From daemon Fri Nov 10 07:03:30 2000



From: Roger Moretz :      rcmoretz-at-excite.com
Date: Fri, 10 Nov 2000 04:58:14 -0800 (PST)
Subject: Re: t-BGE dehydration

Contents Retrieved from Microscopy Listserver Archives
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Jennifer:

I have routinely used an ethanol series straight into Epon (or its various
replacement incarnations) for over 20 years now, with never any poor
embedding issues. Truly dry EtOH is miscible with the epons, but requires
throrough mixing and additional graded steps. For standard tissues blocks,
try 3x in 100% EtOH, followed by 2:1 EtOH:epon, 1:1 EtOH:epon 1:2 EtOH:epon,
and at least 2 more pure epon prior to embedding. The 3 EtOH:epon steps
should be at least 45 min each (1 have used up to 1 hr for dense tissues)
and the pure epon steps can be 30 to 60 min each. I can't speak to the
t-BGE issue, but I developed a skin sensitivity to PO a long time ago, and
worked out the EtOH method to avoid the PO (I originally used acetone for
the dehydration, but had a problem maintaining dry acetone--molecular sieves
work but they can introduce particulate contaminants). Hope this helps.

Roger Moretz, Ph.D.
Dept of Toxicology
Boehringer Ingelheim Pharmaceuticals, Inc.

On Thu, 9 Nov 2000 11:33:32 -0700 (Mountain Standard Time), Jennifer Palmer
wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Does anyone have references and recommendations(or caveats)for
} dehydration of tissue using tBGE? We routinely prep (testis and
} ovary) with PO through Epon812 and want something less toxic that
} still gives good ultrastructure.
}
} ====================================
} Jennifer Palmer
} ARBL
} Colorado State Univ
} Ft. Collins, CO 80523-1683 , USA
} voice:(970)491-1770
} fax:(970)491-3557
}
} jpalmer-at-cvmbs.colostate.edu
} ====================================
}
}





_______________________________________________________
Say Bye to Slow Internet!
http://www.home.com/xinbox/signup.html




From daemon Fri Nov 10 07:59:31 2000



From: mallamaci-at-goodyear.com
Date: Fri, 10 Nov 2000 07:55:00 -0600
Subject: Top-bottom effect

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




w.r.t. double diffraction this effect is covered nicely on pages 278-279 of
Williams and Carter "Transmission Electron Microscopy."





"William F. Tivol" {wft03-at-health.state.ny.us} on 11/09/2000 02:11:26 PM

To: microscopy-at-sparc5.microscopy.com
cc:




Dear List,

Does anyone know a good reference for the origin of the top-bottom effect?
TIA.
Yours,

Bill Tivol
Wadsworth Center
Albany NY
(518) 473-7399 WFT02-at-health.state.ny.us





From daemon Fri Nov 10 08:00:38 2000



From: walck-at-ppg.com
Date: Fri, 10 Nov 2000 07:57:02 -0600
Subject: RE:TEM calibration sample@microscopy.com

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I have discussed John McCaffrey's Mag-I-Cal sample at length in the past.
You can contact South Bay Technology, Electron Microscopy Sciences, or SPI.
I believe all of them have it described on their web sites. It will do
exactly what you want it for and then some.

Try it you'll like it.


-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)



} -----Original Message-----
} From: Debby Sherman [mailto:sherman-at-btny.purdue.edu]
} Sent: Thursday, November 09, 2000 4:44 PM
} To: message to: MSA list
} Subject: TEM calibration sample
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListse} rver/FAQ.html
}
}
} --------------------------------------------------------------
} ---------.
}
}
} Hi all,
} I am looking for a sample to calibrate magnifications of a
} TEM from 50,000X up. I have used catalase in the past.
} However the periodicity of catalase is ~8.7nm. That is too
} large for my present purposes as I am looking at structures
} in the 2-4nm range and want to reduce the margin of error in
} the measurements as much as possible. I would also like a
} sample that is easy to used by inexperienced microscopists in
} the hopes of encouraging more users to calibrate instrument
} magnification on a regular basis. It also needs to be
} relatively in expensive so the loss of a grid is not a catastrophy.
} I would like a standard that would have ~1.0nm lattice.
} I thought I had found the perfect sample in copper
} phthalocyanine which has a spacing in this range.
} Unfortunately the only source of a grid with this crystal has
} discontinued carrying it. I am back to square one. My
} options are to prepare my own grid but need advise on how to
} crystallize the copper phthalocyanine (available from Sigma
} in powder form) or find another crystalline lattice in this
} size range.
} I would appreciate suggestions for alternative crystals
} and/or methods for making the copper phthalocyanine crystals
} to use for this purpose.
} Thanks in advance.
} Debby
}
} Debby Sherman Phone: 765-494-6666
} Life Science Microscopy Facility FAX: 765-494-5896
}
} Purdue University E-mail:
} sherman-at-btny.purdue.edu
} 1057 Whistler Building
} West Lafayette, IN 47907-1057
}
}





From daemon Fri Nov 10 08:02:01 2000



From: Norman_C_Miller-at-res.raytheon.com
Date: Fri, 10 Nov 2000 07:57:59 -0600
Subject: used scanning auger available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



We have a Physical Electronics 570 used Auger/ESCA/SIMS surface analysis
instrument that we must part with. We used this primarily as a scanning Auger,
but also did XPS and some SIMS. This instrument is in working order and has
been
under a service contract until recently. All offers or inquiries are welcome.

Carl Miller





From daemon Fri Nov 10 09:32:07 2000



From: michael shaffer :      epmalab-at-darkwing.uoregon.edu
Date: Fri, 10 Nov 2000 07:24:24 -0800
Subject: RE: Another digital camera twist

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


John writes ...

} ...
} I discovered an interesting quirk of at least this
} Olympus C-2500L digital camera. I suspect that other
} consumer/prosumer cameras would suffer the same fate.
}
} There were four tiles ranging from, say, very light purple
} to dark purple. We'd put them on a black velvet background.
} We had tremendous trouble getting a accurate color. ...
}
} ...
}
} My hypothesis was to add other objects to the scene - sure
} enough, after adding a few other colorful objects at the
} margins of the frame, the appearance of the tiles changed
} dramatically for the better. ...
}
} Certainly this has implications for microscope use of
} today's digital cameras. I don't see a way to turn
} off this feature, at least on this camera.

Could the camera be mis-judging the "white balance"??
On my camera I can disable "automatic", and force
"incandescent", "fluorescent", "daylight", etc.

cheerios, =shAf= :o)
{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {}
Michael Shaffer, R.A. - mshaf-at-darkwing.uoregon.edu
Geological Science's Electron Probe Facility - University of Oregon
http://epmalab.uoregon.edu/





From daemon Fri Nov 10 11:37:18 2000



From: David Cockayne :      david.cockayne-at-materials.oxford.ac.uk
Date: Fri, 10 Nov 2000 17:24:45 -0000
Subject: Monte Carlo Summary

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I recently requested, on this site, advice on programmes for Monte Carlo
simulations of electron scattering. I have been asked by Nestor to
summarise them, to save others asking the same question (and clogging the
site). Here is a summary of many replies I received. I have not accessed
many of them, and pass them on without comment.

David C


1 www.gme.usherb.cs/casino - Gauvin's comprehensive programme


2 site allowing
to do online simulations as well as the possibility of
as well as the possibility of
downloading the program for SEM (and TEM) scattering simulations.
http://callisto.my.mtu.edu/soft/mc/ss_java.html
http://callisto.my.mtu.edu/soft/mc/ (this address for downloading)


3 ftp://www.amc.anl.gov/AMC-3/ANLSoftwareLibrary/3-MASLIB/MONTCARL/

4 www.gme.usherb.cs/casino


5 www.evex.com/evexmontecarlo.exe


6 Try Cnet (www.cnet.com) and enter Monte Carlo into the "search" box. A
page will come up with a list of downloadable programs divided into
purchaseable programs and freeware. No idea whether any of them works as
I haven't tried any. I'm just familiar with Cnet as a source of
downloable software.

7 Try Electron Flight Simulator by Small World, (500)447-3340


email david.cockayne-at-materials.oxford.ac.uk




From daemon Fri Nov 10 15:27:59 2000



From: Kim DeRuyter :      fnksd1-at-uaf.edu
Date: Fri, 10 Nov 2000 12:19:37 -0900
Subject: $ for Ion Milling

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Microscopists,
A researcher here at the University of Alaska is interested in paying to
have some thin sections made from her silicate glass samples. Are there
any labs out there that will contract to do ion milling? She is not on the
list so please reply to Jessica Faust, her E-mail address is
faust-at-gi.alaska.edu
Thanks once again.
Kim DeRuyter




From daemon Fri Nov 10 16:43:41 2000



From: Jorg Wiezorek :      wiezorek+-at-pitt.edu
Date: Fri, 10 Nov 2000 17:38:15 -0500
Subject: Opening- postdoc

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


University of Pittsburgh
Department of Materials Science and Engineering

Postdoctoral Research Position in
Transmission Electron Microscopy


An additional vacancy for a POSTDOCTORAL POSITION has become available in
the area of transmission electron microscopy of thin film data storage
media as part of a collaborative research program between Professors J.M.
Wiezorek and W.A. Soffa, Department of Materials Science and Engineering of
the University of Pittsburgh, and the Seagate Technology. Candidates should
hold a Ph.D. in Materials Science, Physics or a related discipline and must
have extensive hands-on experience in a broad range of imaging, diffraction
and analytical transmission electron microscopy characterization
techniques, as well as with sample preparation methods. Demonstrated
expertise in the preparation of thin film samples suitable for
high-resolution TEM characterization in plan-view and cross-section is
highly desirable. A basic knowledge of magnetism in materials and
experience in instrument development and/or computer image
processing/simulation would be beneficial. The appointment is for one year
in the first instance and is available from December 2000. Applications
from under-represented groups, including minorities, women and people with
disabilities are encouraged.

Interested candidates should send a curriculum vitae, including publication
list, and the names of three referees with postal addresses, telephone
numbers and E-mail addresses to: Prof. J.M. Wiezorek, Department of
Materials Science and Engineering, University of Pittsburgh, 848 Benedum
Hall, Pittsburgh, PA 15261, USA.
Email: wiezorek+-at-pitt.edu




From daemon Fri Nov 10 16:43:44 2000



From: Jorg Wiezorek :      wiezorek+-at-pitt.edu
Date: Fri, 10 Nov 2000 17:38:25 -0500
Subject: Technician Position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Research Specialist Position

The Microcharacterization laboratory in the Department of Materials Science
and Engineering of the University of Pittsburgh has an opening for a
full-time technician in a multiple user facility. The facility's main
instrumentation consists of two Xpert XRD, an XL30FEGSEM with EDS, BSE and
EBSD, and two JEOL TEM's. The successful applicant will have a minimum of a
Bachelor¹s degree and 3 years experience working with scanning electron
microscopes and X-ray diffraction instruments. Responsibilities will
include daily operation and maintenance of the SEM and XRD instrumentation
including the training of users (including students), specimen preparation
and documentation of experimental data. The successful applicant must
demonstrate the necessary organizational, management and communication
skills to efficiently operate in an academic multi-user environment.
Applicants should submit a cover letter describing their experience with
different instrumentation, a resume and contact information for three
references. Salary will be commensurate with the candidate¹s experience.

Resumes should be sent to;
Professor G. H. Meier, Interim Chair
Department of Materials Science and Engineering
848 Benedum Hall
University of Pittsburgh
Pittsburgh, PA 15261

The University of Pittsburgh is an equal opportunity and affirmative action
employer.




From daemon Fri Nov 10 17:02:59 2000



From: John Foust :      jfoust-at-threedee.com
Date: Fri, 10 Nov 2000 16:58:01 -0600
Subject: RE: Another digital camera twist

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 07:24 AM 11/10/00 -0800, michael shaffer wrote:
} Could the camera be mis-judging the "white balance"??
} On my camera I can disable "automatic", and force
} "incandescent", "fluorescent", "daylight", etc.

No, I'd already preset and locked the color temperature
against a white card in the same environment. These other
color adjustments were beyond simple white balance problems.

- John




From daemon Fri Nov 10 17:40:06 2000



From: Roberto Garcia :      rgarcia-at-unity.ncsu.edu
Date: Fri, 10 Nov 2000 18:35:05 -0500
Subject: Scheduling Software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I know that this has been posted before but I lost the link. Does anyone
have a recommendation for WEB scheduling software. Thanks.
______________________________
Roberto Garcia
NCSU/Analytical Instrumentation Facility
Campus Box 7531 Room 318 EGRC
1010 Main Campus Dr.
Raleigh NC 27695-7531
P: (919) 515-8628
F: (919) 515-6965
rgarcia-at-unity.ncsu.edu
http://spm.aif.ncsu.edu/aif
http://spm.aif.ncsu.edu/asm




From daemon Fri Nov 10 18:08:39 2000



From: Douglas Keene :      DRK-at-shcc.org
Date: Fri, 10 Nov 2000 16:05:16 -0800 (Pacific Standard Time)
Subject: Nikon Cool-Pix adaptor for LM

Contents Retrieved from Microscopy Listserver Archives
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This might be of use to those of you wanting to convert
your older stereo and compound microscopes to digital
imaging capability. We recently explored the possibilities
of mounting a Nikon CoolPix 990 (CCD=3.34 million pixels) to
our 14 year old Zeiss stereoscope and Standard microscopes.
Neither has a "C" mount, which is what the Nikon adapter
fits onto. There were several very expensive (} $1,500)
possible solutions, but the most satisfying was one
proposed by Spectra Tech (716-265-4320; e-mail Michael
Specht {mspecht-at-frontiernet.net} ). For about $250, they
were able to provide an adapter which screws directly into
the camera and fits either in the ocular tube or phototube
of the microscopes. Adapters can be purchased for
different tube diameters. Contact them for further
information if interested.

I have no financial interest.

Doug

----------------------
Douglas R. Keene
Associate Investigator
Shriners Hospital Research Facilities
3101 S.W. Sam Jackson Park Road
Portland, Oregon 97201
phone: 503-221-3434
FAX: 503-412-6894 (9-5 PST)
e-mail: DRK-at-shcc.org









From daemon Sat Nov 11 07:43:18 2000



From: femi_adeluyi-at-yahoo.com ()
Date: Sat, 11 Nov 2000 07:36:08 -0600
Subject: low frequency surface microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Email: femi_adeluyi-at-yahoo.com
Name: Adeluyi Olufemi
School: Obafemi AwolowoUniversity, Ile-Ife, Nigeria

Question: I am working on a final year project titled "low frequency
surface microscopy". My project supervisor wants me to use a probe that
consists of a concentric cylinder with a pin in between. This is to
concentrate the field on the surface
to be scanned. With the occurence of cracks, boundaries, etc. on the
surface, the electric field and hence the capacitance is expected to
change. The reflected voltage in the bridge circuit implementation gives a
reflection of the surface characteristic.

My questions go thus (sorry for the preambles):
1. How do I design a probe that can help me to achieve this( what materials
should be used, what is the expected capacitance value, what are the
relevant equations)
2. At what frequencies can this function properly
3. Can you let me into the theory of this method and other similar methods?
4. Can you refer me to some links and/or send some reference materials to me?

---------------------------------------------------------------------------





From daemon Sat Nov 11 07:43:19 2000



From: Sujitha Thavapalachandran :      sujitha24-at-yahoo.com
Date: Sat, 11 Nov 2000 07:32:00 -0600
Subject: Can I please get some info?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear EMU staff,

At the moment, i am doing a physics assignment for my
HSC assessment on the following topic:

"Compare the resolving powers of light and electrom
microscopes and assess the impact of their
development".

I you could help by sending some information, I would
be very grateful. Thank you.

Sujitha



__________________________________________________
Do You Yahoo!?
Thousands of Stores. Millions of Products. All in one Place.
http://shopping.yahoo.com/





From daemon Sun Nov 12 20:13:03 2000



From: G. Macdonald :      glenmac-at-u.washington.edu
Date: Sun, 12 Nov 2000 17:57:31 -0800 (PST)
Subject: Re: Nikon Cool-Pix adaptor for LM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Doug,
does this adapter have a relay lens? What zoom to you use and is there
any vignetting of the field?


I'm buying adapters for a 990 right now.

Regards,
Glen



On Fri, 10 Nov 2000, Douglas Keene wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} This might be of use to those of you wanting to convert
} your older stereo and compound microscopes to digital
} imaging capability. We recently explored the possibilities
} of mounting a Nikon CoolPix 990 (CCD=3.34 million pixels) to
} our 14 year old Zeiss stereoscope and Standard microscopes.
} Neither has a "C" mount, which is what the Nikon adapter
} fits onto. There were several very expensive (} $1,500)
} possible solutions, but the most satisfying was one
} proposed by Spectra Tech (716-265-4320; e-mail Michael
} Specht {mspecht-at-frontiernet.net} ). For about $250, they
} were able to provide an adapter which screws directly into
} the camera and fits either in the ocular tube or phototube
} of the microscopes. Adapters can be purchased for
} different tube diameters. Contact them for further
} information if interested.
}
} I have no financial interest.
}
} Doug
}
} ----------------------
} Douglas R. Keene
} Associate Investigator
} Shriners Hospital Research Facilities
} 3101 S.W. Sam Jackson Park Road
} Portland, Oregon 97201
} phone: 503-221-3434
} FAX: 503-412-6894 (9-5 PST)
} e-mail: DRK-at-shcc.org
}
}
}
}
}
}
}
}




From daemon Sun Nov 12 23:52:16 2000



From: Joan Clark :      j.clark-at-zoology.unimelb.edu.au
Date: Sun, 12 Nov 2000 23:45:57 -0600
Subject: Soft epon-araldite blocks

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have some epon-araldite blocks to section with tiny wallaby embryos
embedded in. Unfortunately the blocks are very soft and the one micron
sections are sticky and very hard to pick up does anyone have a solution
please as I am getting very frustated
Thanks in anticipation
Joan Clark





From daemon Sun Nov 12 23:52:28 2000



From: Gary Gaugler :      gary-at-gaugler.com
Date: Sun, 12 Nov 2000 20:08:57 -0800
Subject: Re: Nikon Cool-Pix adaptor for LM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Depending on the 'scope, it may have an intermediate
lens/optics unit. I am using the CP 990 on Axioskop
and Olympus and the common adapter unit has a
lens.

gg



At 05:57 PM 11/12/00, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From daemon Mon Nov 13 00:58:07 2000



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Mon, 13 Nov 2000 19:59:43 GMT+1200
Subject: JEOL 840 Optical Microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi, listers

Can anyone advise what voltage and polarity should be applied to the
BNC plug on the short co-axial cable labelled "OM1" which issues from
the flange of the JEOL OM on an 840?

It's something to do with an electron deflector or collector.

tia

rtch


Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand



From daemon Mon Nov 13 05:04:42 2000



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Mon, 13 Nov 2000 11:00:02 +0000 (GMT Standard Time)
Subject: Re: Soft epon-araldite blocks

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have read here that you can put the blocks back in the
oven at a higher temperature. Why not try this with an
unwanted portion of a block?

Dave


On Sun, 12 Nov 2000 23:45:57 -0600 Joan Clark
{j.clark-at-zoology.unimelb.edu.au} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I have some epon-araldite blocks to section with tiny wallaby embryos
} embedded in. Unfortunately the blocks are very soft and the one micron
} sections are sticky and very hard to pick up does anyone have a solution
} please as I am getting very frustated
} Thanks in anticipation
} Joan Clark
}
}
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"




From daemon Mon Nov 13 06:25:17 2000



From: Jim at ProSciTech :      jim-at-proscitech.com
Date: Mon, 13 Nov 2000 22:21:20 +1000
Subject: RE: Soft epon-araldite blocks

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Try placing the blocks back in the oven at anything up to 100C for several
hours, or even overnight.
Remember that the blocks harden after removal from the oven for up to two
hours; not just the couple of minutes it takes to physically cool.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Monday, November 13, 2000 3:46 PM, Joan Clark
[SMTP:j.clark-at-zoology.unimelb.edu.au] wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I have some epon-araldite blocks to section with tiny wallaby embryos
} embedded in. Unfortunately the blocks are very soft and the one micron
} sections are sticky and very hard to pick up does anyone have a solution
} please as I am getting very frustated
} Thanks in anticipation
} Joan Clark
}
}




From daemon Mon Nov 13 09:46:18 2000



From: michael shaffer :      epmalab-at-darkwing.uoregon.edu
Date: Mon, 13 Nov 2000 07:39:50 -0800
Subject: RE: Nikon Cool-Pix adaptor for LM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Doug writes ...

} This might be of use to those of you wanting to convert
} your older stereo and compound microscopes to digital
} imaging capability. We recently explored the possibilities
} of mounting a Nikon CoolPix 990 (CCD=3.34 million pixels) to
} our 14 year old Zeiss stereoscope and Standard microscopes.
} Neither has a "C" mount, which is what the Nikon adapter
} fits onto. There were several very expensive (} $1,500)
} possible solutions, but the most satisfying was one
} proposed by Spectra Tech ... For about $250, they
} were able to provide an adapter which screws directly into
} the camera and fits either in the ocular tube or phototube
} of the microscopes. Adapters can be purchased for
} different tube diameters. ...

When you say your were "satisfied", what features/characteristics
would you care to mention? Did the amount of field-of-view vary? How
much of the field were you able to capture?
(If satisfaction essentially boils down to price/quality, a similarly
priced and quality eyepiece adapter is also available from Optem
International {www.optemintl.com} ... however, I am testing their
C-mount adapter).

cheerios, =shAf= :o)

{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {}
Michael Shaffer, R.A. - mshaf-at-darkwing.uoregon.edu
Geological Science's Electron Probe Facility - University of Oregon
http://epmalab.uoregon.edu/





From daemon Mon Nov 13 10:16:13 2000



From: Russell E. Cook :      recook-at-anl.gov
Date: Mon, 13 Nov 2000 10:12:23 -0600
Subject: Re: Scheduling Software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I've been looking at some of these sites lately for web-based calendars. I
don't necessarily recommend any of them. You'll see that each has
shortcomings.

1. http://www.webevent.com (Can be run on all sorts of operating systems.
Commercial software. Lower prices for non-profit institutions.)

2. http://srv.emunit.unsw.edu.au (Excellent, although it is probably only
for Windows servers. Some accounting capabilities. Not commercial
software. About $500 US.)

3. http://www.webprog.com/appoint/freetest.html (About $60 US. Requires a
Windows server. Basic.)

4. http://cmmserv.mrl.uiuc.edu/calendar (UserNumber = 2399, password =
DEMO, instrument = Zeiss DSM-960) (Windows server required.)
} -----------------------------------------------------------------------.
}
} I know that this has been posted before but I lost the link. Does anyone
} have a recommendation for WEB scheduling software. Thanks.
} ______________________________
} Roberto Garcia
} NCSU/Analytical Instrumentation Facility
} Campus Box 7531 Room 318 EGRC
} 1010 Main Campus Dr.
} Raleigh NC 27695-7531
} P: (919) 515-8628
} F: (919) 515-6965
} rgarcia-at-unity.ncsu.edu
} http://spm.aif.ncsu.edu/aif
} http://spm.aif.ncsu.edu/asm


----------------------------------------------------------------------
Russell E. Cook, Ph.D.
Electron Microscopy Center
Argonne National Laboratory
Materials Science Division, Building 212
9700 South Cass Avenue
Argonne, IL 60439-4838
(630)252-7194
FAX: (630)252-4289
recook-at-anl.gov





From daemon Mon Nov 13 10:26:55 2000



From: John Basgen :      basgen-at-maroon.tc.umn.edu
Date: Mon, 13 Nov 2000 10:23:03 -0600
Subject: Skin Biopsies

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


To those doing EM on skin biopsies:

We have been asked to embed some human skin biopsies for electron microscopy.
We used our routine protocol for processing kidney biopsies which includes
glutaraldehyde fixation, dehydration through ethanol and acetone and embeddment
in PolyBed 812. Our protocol resulted in good embeddment all the layers of the
skin except for the stratum corneum where there were numberous holes in the
PolyBed. Is it possible to get good embeddment of this keratinized zone?

Thanks for any suggestion,

John

John M. Basgen
Department of Pediatrics
University of Minnesota
Mayo Mail Code 491
420 Delaware Street SE
Minneapolis, MN 55455
USA
Phone: 612-625-7979
FAX: 612-626-2791
E-mail: basgen-at-tc.umn.edu




From daemon Mon Nov 13 15:39:45 2000



From: Hyres, James W. :      jwhyres-at-mcdermott.com
Date: Mon, 13 Nov 2000 15:29:07 -0600
Subject: RE: Nikon Cool-Pix adaptor for LM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does anyone have experience with the 990 under low light conditions? We
normally shoot brightfield on our inverted metallograph, but there is the
occasional need for darkfield and DIC.

Thanks in advance,
Jim Hyres

-----Original Message-----
} From: michael shaffer [mailto:epmalab-at-darkwing.uoregon.edu]
Sent: Monday, November 13, 2000 10:40 AM
To: DRK-at-shcc.org; Microscopy-at-sparc5.microscopy.com


Doug writes ...

} This might be of use to those of you wanting to convert
} your older stereo and compound microscopes to digital
} imaging capability. We recently explored the possibilities
} of mounting a Nikon CoolPix 990 (CCD=3.34 million pixels) to
} our 14 year old Zeiss stereoscope and Standard microscopes.
} Neither has a "C" mount, which is what the Nikon adapter
} fits onto. There were several very expensive (} $1,500)
} possible solutions, but the most satisfying was one
} proposed by Spectra Tech ... For about $250, they
} were able to provide an adapter which screws directly into
} the camera and fits either in the ocular tube or phototube
} of the microscopes. Adapters can be purchased for
} different tube diameters. ...

When you say your were "satisfied", what features/characteristics
would you care to mention? Did the amount of field-of-view vary? How
much of the field were you able to capture?
(If satisfaction essentially boils down to price/quality, a
similarly
priced and quality eyepiece adapter is also available from Optem
International {www.optemintl.com} ... however, I am testing their
C-mount adapter).

cheerios, =shAf= :o)

{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {}
Michael Shaffer, R.A. - mshaf-at-darkwing.uoregon.edu
Geological Science's Electron Probe Facility - University of Oregon
http://epmalab.uoregon.edu/





From daemon Mon Nov 13 15:39:51 2000



From: Jim Mabon :      mabon-at-uiuc.edu
Date: Mon, 13 Nov 2000 15:36:08 -0600
Subject: Need Manuals etc. for Kevex 770 XRF

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am looking for manuals for a Kevex 770 XRF. In particular
we would be interested in copies of the circuit diagrams and
documentation for the XRF unit and the Detector/Pulse
Processor/ADC interface. The Kevex DELTA computer
and software is not really of interest, since we would like to
change it to a PC platform.

Also, any suggestions of other listservers, etc. to look would
be appreciated.

Thanks in Advance,
Jim Mabon

__________________________________________
James C. Mabon, Ph.D.
Research Electron Microscopist
Materials Research Laboratory
University of Illinois at Urbana-Champaign
104 S. Goodwin Avenue
Urbana, IL 61801
http://ntweb.mrl.uiuc.edu/cmm
__________________________________________





From daemon Mon Nov 13 17:36:51 2000



From: Bob Wise :      wise-at-vaxa.cis.uwosh.edu
Date: Mon, 13 Nov 2000 17:30:07 -0500
Subject: Color scanning EM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


OK, I'll bite. What the heck is color scanning EM, as referenced in the
title of the following article?

TIA

Bob

Histochemistry of food tissue by colour scanning electron microscopy
Mitsuhiko Yamada, Masako Nishimura, Takeo Suzuki, Shigeru Kawamata, Eisaku
Oho, and Toshiaki Kimura, pp. 503-507. Journal of Electron Microscopy vol
49(3)

Robert R. Wise, Ph.D.
Associate Professor of Plant Physiology
Department of Biology and Microbiology
University of Wisconsin Oshkosh
Oshkosh, WI 54901
tele: (920) 424-3404
fax: (920) 424-1101
wise-at-uwosh.edu
http://www.uwosh.edu/departments/biology/wise/wise.html



From daemon Mon Nov 13 18:56:15 2000



From: Ron Veil :      veilcs-at-juno.com
Date: Mon, 13 Nov 2000 16:49:00 -0800
Subject: looking for an IGP for a Philips TEM410

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am interested in finding an IGP for a Philips (T)EM410 that can be
rebuilt / refurbished. Anybody got one lying around?

TIA
Ron Veil

Veil Electron Instrument Lab Customer Services
Tel: (209) 521-3332
FAX: (209) 521-3033
e-mail: veilcs-at-earthlink.net
________________________________________________________________
YOU'RE PAYING TOO MUCH FOR THE INTERNET!
Juno now offers FREE Internet Access!
Try it today - there's no risk! For your FREE software, visit:
http://dl.www.juno.com/get/tagj.



From daemon Mon Nov 13 20:40:03 2000



From: Trevor Sewell :      sewell-at-uctvms.uct.ac.za
Date: Tue, 14 Nov 2000 03:34:01 +0200
Subject: Re: Scheduling Software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have recently experimented with
www.ezbook.com

It is entirely web based and hosted on their
server. It has some useful features.

Trevor







From daemon Mon Nov 13 22:58:11 2000



From: mark.talbot-at-studentmail.newcastle.edu.au (MARK TALBOT)
Date: Tue, 14 Nov 2000 15:50:08 +1100 (EST)
Subject: SEM: removal of cytoplasm

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi everyone,

I am interested in removing the cytoplasm from plant cells for viewing cell
walls by SEM. the cells are epidermal cells of bean cotyledons, and I was
wanting a simple and rapid method to get rid of the cytoplasm which doesn't
harm the cell wall.

Also, I was wondering if anyone knew of an SEM image analysis program that
can count things, do nearest-neighbour analysis, density measurements and
so on. The things I want to measure are quite variable in shape and size,
so it might be a little difficult.

Thanks for your time.

Mark Talbot





From daemon Tue Nov 14 07:57:02 2000



From: beth-at-dogwood.botany.uga.edu (Beth Richardson)
Date: Tue, 14 Nov 2000 08:54:12 -0500
Subject: Re: SEM: removal of cytoplasm

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hi Mark,
We use a commercially available washing powder called Ariel (Procter and
Gamble) to remove the cytoplasm. Use a 5% (w/v) aqueous solution - it
contains a Bacillus subtilis derived protease.
References:
Honegger, R. 1985. Scanning electron microscopy of the fungus-plant
interface: a simple preparative technique. Trans Br. Mycol. Soc. 84:
530-533.

Mims et al. 1989. Ultrastructure of the haustorium of the peanut late leaf
spot fungus Cerosporidium personatum. Can. J. Bot. 67: 1198-1202.

It worked quite well for us...good luck,
Beth

} Hi everyone,
}
} I am interested in removing the cytoplasm from plant cells for viewing cell
} walls by SEM. the cells are epidermal cells of bean cotyledons, and I was
} wanting a simple and rapid method to get rid of the cytoplasm which doesn't
} harm the cell wall.

**************************************
Beth Richardson
EM Lab Coordinator
Botany Department
University of Georgia
Athens, GA 30602

Phone - (706) 542-1790
FAX - (706) 542-1805
Email - beth-at-dogwood.botany.uga.edu

"Between the two evils,
I always pick the one I never tried before". Mae West (1893-1980)
**************************************





From daemon Tue Nov 14 10:26:15 2000



From: MICHAEL DELANNOY :      delannoy-at-mail.jhmi.edu
Date: Tue, 14 Nov 2000 11:06:43 -0500 (EST)
Subject: Re: Skin Biopsies

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


John,
Here is a protocol from Pierre Coloumbe's lab (thanks Stacy)
for embedding pieces of skin, see how it compares to yours

Fix in 2% GA, 1% PF, 0.1M sodium cacodylate (caco) pH 7.2 overnight 4 degrees

3-4 washes same buffer 5 min ea

Post-fix 1% OsO4/0.1 M caco 1-1.5 hr room temp

2 washes 0.1 M caco

3-4 washes ddh20 5 min ea

50% ETOH (tissue can be stored 4 degrees)

Dehydration:

50% ETOH 5 min
70% " "
90% " "
95% " "

2 changes 100% (unopened bottle 200 proof) 10 min ea

3 changes 100% propylene oxide (do not let tissue contact air, keep submerged
at all times!!!) 10 min ea

1:1 propylene oxide:Epon with catalyst 3-5 hrs

1:2 " " " " " " overnight shake

Fresh change Epon with cataylyst, shake all day, transfer tissue into
new vials changing Epon and shake overnight

Fresh Epon with cataylyst, rubber mold embed, cure 60-65 degrees for 3-4
days until hard.

Good Luck,

Michael Delannoy
Johns Hopins School of Medicine
Microscopy Facility





From daemon Tue Nov 14 10:41:05 2000



From: Reinhard Windoffer :      windoff-at-mail.uni-mainz.de
Date: Tue, 14 Nov 2000 17:37:37 +0100
Subject: TILL PHOTONICS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


hello,
for life cell microscopy of fp-tagged cells we consider to buy a TILL
PHOTONICS imaging system in combination with a Olympus microscope. We
would appreciate any comments on this system or possible alternatives.
Beside single channel recording we are also interested in multiple color
imaging including FRET. We would like to hear comments on general system
performance, usability, flexibility, support, and problems with the
system.


Reinhard Windoffer


--
Dr. Reinhard Windoffer phone ++49-(0)6131 39 23720
Universitaet Mainz fax ++49-(0)6131 39 23719
Anatomisches Institut internet windoff-at-mail.uni-mainz.de
Becherweg 13 www.uni-mainz.de/windoff
D-55099 Mainz





From daemon Tue Nov 14 11:38:06 2000



From: michael shaffer :      epmalab-at-darkwing.uoregon.edu
Date: Tue, 14 Nov 2000 09:30:14 -0800
Subject: RE: Nikon Cool-Pix adaptor for LM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



James writes ...

} Does anyone have experience with the 990 under low light
} conditions? We normally shoot brightfield on our
} inverted metallograph, but there is the
} occasional need for darkfield and DIC.
} ...

With ISO senstivity selectable (100,200,400), and manual maximum
aperture and a manual selectable shutter up to 8sec (including bulb),
should allow anything you can "see" thru the eyepieces. But I do
suspect an increase in noise as you approach the limits, and you might
want a trinoc head which can throw 100% of the light at the camera.

cheerios, =shAf= :o)

{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {}
Michael Shaffer, R.A. - mshaf-at-darkwing.uoregon.edu
Geological Science's Electron Probe Facility - University of Oregon
http://epmalab.uoregon.edu/




From daemon Tue Nov 14 14:39:09 2000



From: Michael Jarnik :      M_Jarnik-at-fccc.edu
Date: Tue, 14 Nov 2000 15:32:08 -0500
Subject: EM technician position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Job Opening

Electron Microscopy Facility at Fox Chase Cancer Center is seeking a
motivated individual for a Technician/Research Assistant position.

Required qualifications: B.S. or M.S. in biology, 2+ years of experience
in biological electron microscopy.

Responsibilities include sample preparation and electron microscopy
(TEM/SEM), dark room work, computer image processing, report
preparation. The great variability of work due to collaborations with
large number of laboratories provides exceptional opportunity for
professional growth.

We offer a competitive salary commensurate with experience, an excellent
benefit package (health/dental insurance, pension plan, paid vacation)
and a very friendly working environment.

For confidential consideration, please send a CV including a statement
of experience to:

Dr. Michael Jarnik
Fox Chase Cancer Center
EM Facility
7701 Burholme Avenue
Philadelphia, PA 19111
e-mail: m_jarnik-at-fccc.edu






From daemon Tue Nov 14 15:19:08 2000



From: John Hardy :      jhardy-at-coh.org
Date: Tue, 14 Nov 2000 11:26:18 -0800
Subject: LKB Ultrotome parts?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



We are looking for a replacement belt for the control unit of an LKB
Ultrotome III 8800. Any ideas what-so-ever? It's only 25+ yrs old! Thanks
in advance.

John Hardy
City of Hope Medical Center
Duarte, CA
(626) 301-8265

jhardy-at-coh.org





From daemon Tue Nov 14 16:02:47 2000



From: Tamara Howard :      howard-at-cshl.org
Date: Tue, 14 Nov 2000 16:58:24 -0500 (EST)
Subject: Re: LKB Ultrotome parts?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


If you have a mechanical/instrument shop at your institution, ask the
gurus there for belt material. All you need to be careful of is matching
the diameter of the new belt with the old one. I've done this in the past
and it works really well. If there is no shop, go to a trusted
hardware/parts store with the old belt and ask for a similar replacement.

Giant rubber bands do not work......(but it was worth a try!)

Tamara Howard
CSHL


On Tue, 14 Nov 2000, John Hardy wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} We are looking for a replacement belt for the control unit of an LKB
} Ultrotome III 8800. Any ideas what-so-ever? It's only 25+ yrs old! Thanks
} in advance.
}
} John Hardy
} City of Hope Medical Center
} Duarte, CA
} (626) 301-8265
}
} jhardy-at-coh.org
}
}
}
}





From daemon Tue Nov 14 18:46:11 2000



From: Norman_C_Miller-at-res.raytheon.com
Date: Tue, 14 Nov 2000 18:40:56 -0600
Subject: used scanning acoustic microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


All,

We have a ten year old Panametrics Hyscan scanning acoustic microscope that we
must part with. This includes an old computer, controller, scan drive, and
tank.
Does anyone have an interest in the system or part of it?

Carl Miller





From daemon Wed Nov 15 01:11:59 2000



From: Emma Bonino :      emmabonino-at-elezioniradicali.org
Date: Wed, 15 Nov 2000 01:32:10 +0100
Subject: Un "sondaggio" e 7 buone azioni...

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Salve, sono Emma Bonino, e le propongo un "sondaggio" e 7 iniziative online.
Grazie e buona... azione!

Quali alleanze per le elezioni politiche ?
"SONDAGGIO":
http://www.radicali.it/action/sondaggio/

Azioni online:

ABORTO E CONTRACCEZIONE
Per introdurre in Italia pillola abortiva RU486
http://www.radicali.it/action/ru486/

FREE SOFTWARE
Petizione europea contro la brevettabilita' del software
http://www.radicali.it/action/freesoft/

LEGALIZZAZIONE DROGHE
Sostegno a Pannella e ai radicali sotto processo per disobbedienza civile
http://www.radicali.it/action/legalize/

FIRMA DIGITALE E VOTO ELETTRONICO
Richiesta al Governo per la legalita' delle elezioni politiche
http://www.radicali.it/action/digital/

CONTRO LA TELEVISIONE DI STATO
Per abolire la concessione unica radiotelevisiva
http://www.radicali.it/action/rai/

CONTRO IL SINDACATO DI STATO
Per l'abolizione del sistema di rinnovo automatico dell'iscrizione sindacale
http://www.radicali.it/action/sindacato/

LIBERALIZZAZIONE TELECOMUNICAZIONI
Per l'abolizione del canone Telecom
http://www.radicali.it/action/telecom/

Su questi ed altri temi si confronteranno tra pochi giorni anche le liste
delle elezioni radicali online.
Per partecipare e votare, clicca qui :
http://www.radicali.it/action/register

***
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desidera maggiori informazioni o se intende cancellarsi, puo cliccare qui:
http://www.radicali.it/action/privacy/






From daemon Wed Nov 15 04:53:24 2000



From: Emma Bonino :      emmabonino-at-elezioniradicali.org
Date: Wed, 15 Nov 2000 01:32:10 +0100
Subject: Un "sondaggio" e 7 buone azioni...

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Salve, sono Emma Bonino, e le propongo un "sondaggio" e 7 iniziative online.
Grazie e buona... azione!

Quali alleanze per le elezioni politiche ?
"SONDAGGIO":
http://www.radicali.it/action/sondaggio/

Azioni online:

ABORTO E CONTRACCEZIONE
Per introdurre in Italia pillola abortiva RU486
http://www.radicali.it/action/ru486/

FREE SOFTWARE
Petizione europea contro la brevettabilita' del software
http://www.radicali.it/action/freesoft/

LEGALIZZAZIONE DROGHE
Sostegno a Pannella e ai radicali sotto processo per disobbedienza civile
http://www.radicali.it/action/legalize/

FIRMA DIGITALE E VOTO ELETTRONICO
Richiesta al Governo per la legalita' delle elezioni politiche
http://www.radicali.it/action/digital/

CONTRO LA TELEVISIONE DI STATO
Per abolire la concessione unica radiotelevisiva
http://www.radicali.it/action/rai/

CONTRO IL SINDACATO DI STATO
Per l'abolizione del sistema di rinnovo automatico dell'iscrizione sindacale
http://www.radicali.it/action/sindacato/

LIBERALIZZAZIONE TELECOMUNICAZIONI
Per l'abolizione del canone Telecom
http://www.radicali.it/action/telecom/

Su questi ed altri temi si confronteranno tra pochi giorni anche le liste
delle elezioni radicali online.
Per partecipare e votare, clicca qui :
http://www.radicali.it/action/register

***
PS Questa email e stata inviata nel rispetto della legge sulla privacy; se
desidera maggiori informazioni o se intende cancellarsi, puo cliccare qui:
http://www.radicali.it/action/privacy/






From daemon Wed Nov 15 07:03:10 2000



From: Gunnar Kopstad :      gunnar.kopstad-at-medisin.ntnu.no
Date: Wed, 15 Nov 2000 13:57:34 +0100
Subject: EDX Kevex 7000 spare parts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello.

We still have a Kevex 7000 system connected to a LSI11/23 computer. Now,
we have problem with booting the computer when the analyser is connected to
the PDP-bus. We think, but we don't know, if we ahve a problem with the
Kevex Q23 board,which interfaces the analyser to the bus.
However, do anybody have other suggestions? Or even better, is there a Q23
board out there?

Ypurs Sincererly Gunnar Kopstad


Vennlig Hilsen
dr.ing Gunnar Kopstad
overingeniør Avd f Patologi, Rit

tlf. 73 86 86 56, tlf. privat 72 88 79 58
pers.s. 967 75 026



From daemon Wed Nov 15 07:29:02 2000



From: Emma Bonino :      emmabonino-at-elezioniradicali.org
Date: Wed, 15 Nov 2000 01:32:10 +0100
Subject: Un "sondaggio" e 7 buone azioni...

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Salve, sono Emma Bonino, e le propongo un "sondaggio" e 7 iniziative online.
Grazie e buona... azione!

Quali alleanze per le elezioni politiche ?
"SONDAGGIO":
http://www.radicali.it/action/sondaggio/

Azioni online:

ABORTO E CONTRACCEZIONE
Per introdurre in Italia pillola abortiva RU486
http://www.radicali.it/action/ru486/

FREE SOFTWARE
Petizione europea contro la brevettabilita' del software
http://www.radicali.it/action/freesoft/

LEGALIZZAZIONE DROGHE
Sostegno a Pannella e ai radicali sotto processo per disobbedienza civile
http://www.radicali.it/action/legalize/

FIRMA DIGITALE E VOTO ELETTRONICO
Richiesta al Governo per la legalita' delle elezioni politiche
http://www.radicali.it/action/digital/

CONTRO LA TELEVISIONE DI STATO
Per abolire la concessione unica radiotelevisiva
http://www.radicali.it/action/rai/

CONTRO IL SINDACATO DI STATO
Per l'abolizione del sistema di rinnovo automatico dell'iscrizione sindacale
http://www.radicali.it/action/sindacato/

LIBERALIZZAZIONE TELECOMUNICAZIONI
Per l'abolizione del canone Telecom
http://www.radicali.it/action/telecom/

Su questi ed altri temi si confronteranno tra pochi giorni anche le liste
delle elezioni radicali online.
Per partecipare e votare, clicca qui :
http://www.radicali.it/action/register

***
PS Questa email e stata inviata nel rispetto della legge sulla privacy; se
desidera maggiori informazioni o se intende cancellarsi, puo cliccare qui:
http://www.radicali.it/action/privacy/






From daemon Wed Nov 15 07:38:43 2000



From: Debby Sherman :      sherman-at-btny.purdue.edu
Date: Tuesday, November 14, 2000 8:57 PM
Subject: Fwd: TEM calibration sample

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Carol,
Thanks for the post. Since I want the grid for calibration, not resolution, I needed something with a lattice which was visible at magnifications from about 50,000X up. I may have found a couple of possiblilities. Esbestos fiber grids with 1.3nm periodicity are available commercially. Also Ted Pella has arranged to obtain Cu-phthalocyanine grids (which had been in their catalog but were discontinued) with a 1.0nm periodicity. I think that either or both of these should do the trick.
Debby


--------------------------------------








From daemon Wed Nov 15 08:15:50 2000



From: George_Munzing-at-ENGELHARD.COM
Date: Wed, 15 Nov 2000 09:09:32 -0500
Subject: LKB Ultrotome parts?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


You may want to check with the following:

Microscopical Optical Consulting (MOC) Inc.
Helmut Patzig
P.O Box 586
Valley Cottage, NY 10989
(914) 268-6450
MOCLeica-at-aol.com

They had picked up the Leica service and were able to help with my old LKB
system.

Good Luck!


George R. Munzing Jr
Strategic Technologies Group
Engelhard Corporation
25 Middlesex/Essex Tpk.
Iselin, NJ 08830
(732) 205-7030












"John Hardy" {jhardy-at-coh.org} on 11/14/2000 02:26:18 PM

To: "Microscopy" {Microscopy-at-sparc5.microscopy.com}
cc:



We are looking for a replacement belt for the control unit of an LKB
Ultrotome III 8800. Any ideas what-so-ever? It's only 25+ yrs old! Thanks
in advance.

John Hardy
City of Hope Medical Center
Duarte, CA
(626) 301-8265

jhardy-at-coh.org









From daemon Wed Nov 15 08:54:49 2000



From: Ryna B. Marinenko :      ryna.marinenko-at-nist.gov
Date: Wed, 15 Nov 2000 09:55:23 -0500
Subject: MAS Special Topics Workshop at NIST

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html





MAS SPECIAL TOPICS WORKSHOP
Dale Newbury and Ryna Marinenko

The NIST Microanalysis Research Group and the Microbeam Analysis Society
will co-sponsor an MAS Special Topics Workshop, "Understanding the Accuracy
Barrier in Quantitative Electron Probe Microanalysis and the Role of
Standards," that will be held at NIST in Gaithersburg, Maryland October
15-18, 2001. This event is part of the 2001 celebration of the NIST
Centennial. The limited attendance workshop will deal with the
experimental and theoretical factors that currently limit the accuracy of
quantitative results to approximately 2% relative. The topics will
include sample and instrument parameters, counting statistics, correction
procedures, individual matrix correction parameters, etc. In addition, one
or more sessions will be specifically devoted to discussions on
microanalysis standards. Invited speakers will be expected to provide a
short (6 pages, maximum) manuscript of their paper at the time of the
meeting. These manuscripts will be subsequently published in a monograph.
The intent of the meeting and monograph is to provide detailed information
on accuracy limits and on acceptable standards procedures in quantitative
microprobe analysis that is presently not readily available in the
literature.

Below is a proposed outline of presentations. Please feel free to suggest
possible speakers (don't be afraid to call your own number!) and additional
topics. We don't expect to be able to address all significant topics, but
rather hope to initiate an ongoing dialogue that will be addressed by
periodic special workshops on this topic.

We are now preparing a list of invited speakers, and we are actively
soliciting volunteers who would like to be considered to present a talk,
especially on microanalysis standards. Not all types or groups of
microanalysis standards have been considered in this list below. We would
like to cover as much of the periodic table as possible.

In addition, if you are simply interested in attending the meeting, please
send your name, address, telephone number, email, and FAX to receive future
information.


Factors that play a significant role in the 2% relative accuracy barrier
… Experimental factors
… Instrument stability: how good is it really?
… Electron beam performance
… WDS peak reproducibility
… EDS performance
… The role of the specimen
… Preparation issues
… Stability (beam damage)
… Charging
… Spectral processing
… Peak deconvolution in WDS
… Extracting intensities from EDS spectra (peak overlaps)
… Quantitative matrix corrections
… How well do matrix correction schemes work on data outside their native
databases?
… What is missing? Where is research needed? Who will do it?


Standards in Quantitative EPMA
… Procedures for Preparation of Bulk Standards specimens for Quantitative EPMA
… Sources, Characterization, and Validation of Standard Materials
… Commercial, private, institutional sources
… Composition, Microheterogeneity, Stability, etc.
… Traceability, certification, intercomparisons, round robins
… Industry/Technology Specific Standards
… Mineralogy and Geology - sources of standard specimens and preparation
procedures
… Pure Element and Oxide Standards
… Useful Stoichiometric Compounds - crystalline, amorphous, sintered
… Sulphides, selenides, and tellurides
… Rare Earth Elements
… Halides
… Alkali Earth Elements
… Low-atomic number elements, C, N, B, Be
… Glass standards

Contact:
dale.newbury-at-nist.gov, (301)975-3921, FAX: (301)417-1321
ryna.marinenko-at-nist.gov, (301)975-3901, FAX: (301)417-1321
Ryna B. Marinenko
NIST
100 Bureau Drive; Stop 8371
Gaithersburg, MD 20899-8371
Tele: (301)975-3901
FAX: (301)417-1321
email: ryna.marinenko-at-nist.gov



From daemon Wed Nov 15 10:51:05 2000



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Wed, 15 Nov 2000 10:43:24 -0600
Subject: Parts for LKB ultramicrotomes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


My last contact information for LKB belts and service is:

Norm Woodside for belts at about $50 apiece. Fax: 410-744-8522.

Pat Capogrosi for service. Telephone: 410-744-1580.

This information is over a year old, but worth a try. Also, the drive belts
can be almost duplicated by belts that cost about $8-10 each, if you have a
good machine or instrument repair shop with comprehensive belt catalogs.
Here are some specs you can try:

Belt thickness: 0.5mm.
Outer circumference: 18cm.
Width: 5mm.
Number of teeth: 39.
Size of teeth: 1mm by 5mm.
Height of teeth: 2mm.
(Thanks to Preston Stogsdill for this information.)

I've never been able to find one that EXACTLY fits, but I've gotten close
enough that the microtomes work fine, if you don't mind a little "bump" as
you turn the knob to raise and lower the specimen arm. The problem is
finding the exact number of teeth. The bump doesn't translate into choppy
cuts, but you might feel it in the knob itself.

Hope this helps.

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/






From daemon Wed Nov 15 13:04:15 2000



From: kszaruba1-at-mmm.com
Date: Wed, 15 Nov 2000 12:58:51 -0600
Subject: Re: Skin Biopsies

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Hi John,

Here is a protocol I developed over a few years of trying to examine stratum
corneum (it was based of course on published protocols and suggestions from this
listserve -- nothing novel). The only big differences between this and Michael
Delannoy's reply are the extended dehydration and infiltration times, along with
the use of ruthenium tetroxide. I found the latter to be a must if you are
interested in preserving the intercellular lipids of the stratum corneum. Also
I found the use of a dermatome led to increased splitting off of the outermost
layers of corneocytes, so simply excising small pieces of skin was better.
Hope this helps,

Karen Zaruba
3M Company, St. Paul, MN
kszaruba1-at-mmm.com

Preparation of Skin for TEM examination of Stratum Corneum

At necropsy, excise the skin with a minimum of stretching or force.
Pin out skin in containers of half-strength Karnovsky?s fixative, which is 2%
glutaraldehyde + 2% paraformaldehyde in cacodylate Buffer (0.15M sodium
cacodylate, pH 7.4, with 0.1 M sucrose). After about 1 hr. the outer tissue
should be trimmed away. The central areas should be divided into final
embedding sizes (1 x 1 x 3 mm) by cutting over a piece of dental wax using a
Personna razor blade.
Continue fixation for a total of 2-12 hr., then wash in buffer 3 x 5 min.
Post-fix in 1% osmium tetroxide in buffer for 30 min. Wash in buffer without
sucrose 2 x 5 min., then briefly in distilled water.
Post-fix in 0.2% ruthenium tetroxide (with or w/o 0.25% potassium
ferricyanide) in 0.1 M cacodylate buffer without sucrose for time indicated.
Rinse in buffer or distilled water, 3 x 5 min. (Use buffer if being held
overnight at this stage.) Note: RuO4 reacts violently with filter paper and
alcohols. Also reacts with benzene rings and organics.
Dehydrate in ethanol series, 25-35 min. each step from 30% through 95%, then 3
x 20 min. in 100%.
Incubate in tert-Butyl Glycidyl Ether, 2 x 30 min.
Infiltrate in a mixture of 2 parts t-BGE to 1 part Spurr?s resin for 1-2 hrs.
Then follow with 1 part t-BGE to 2 parts Spurr?s resin overnight.
Continue to infiltrate in Spurr?s resin alone, 3 changes over 6-8 hrs.
Embed in fresh resin in flat molds, orienting to allow cross-sectioning of the
epidermis. Polymerize at approximately 58 C for 1-3 days.
To visualize lipid lamellae, do not post-stain. For best contrast of
desmosomes and cellular detail, post-stain with standard Tannin/Uranyl Acetate
stain followed by Reynold?s Lead Citrate.

-----------------------------------------------------------------------
John Basgen wrote:

To those doing EM on skin biopsies:

We have been asked to embed some human skin biopsies for electron microscopy.
We used our routine protocol for processing kidney biopsies which includes
glutaraldehyde fixation, dehydration through ethanol and acetone and embeddment
in PolyBed 812. Our protocol resulted in good embeddment all the layers of the
skin except for the stratum corneum where there were numberous holes in the
PolyBed. Is it possible to get good embeddment of this keratinized zone?

Thanks for any suggestion,

John

John M. Basgen
Department of Pediatrics
University of Minnesota
Mayo Mail Code 491
420 Delaware Street SE
Minneapolis, MN 55455
USA
Phone: 612-625-7979
FAX: 612-626-2791
E-mail: basgen-at-tc.umn.edu






From daemon Wed Nov 15 13:29:40 2000



From: kszaruba1-at-mmm.com
Date: Wed, 15 Nov 2000 13:26:22 -0600
Subject: ESEM Biohazards?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Hello fellow microscopists,

As part of a hazard review on our new ESEM, many questions were raised regarding
the observation of biohazards. We won't be looking at actual human pathogens
(although maybe others do?? CDC/NIH??). However, just looking at unfixed human
skin puts us under the Class 2 category. Also we'll be observing non-pathogenic
(opportunisitic) bacteria. Here are the questions:

Are there conditions inside the ESEM that would favor the creation of an
aerosol from the sample?
Assuming any of the sample becomes detached/airborn inside the chamber, would
pumping down to higher vacuum prior to opening the door remove them (seems
logical that it would)?
How do you dispose of pump oil containing microbes/biohazards? Would
anything capable of infecting humans even survive in pump oil? (Remember
we're talking about viruses as well as bacteria here).
Finally, would the chamber walls, etc. need to be decontaminated
periodically?

If anyone has gone through this sort of safety review and generated a protocol,
we'd love to benefit from your hard work!! Otherwise we'd appreciate any
suggestions/opinions.

Thanks as always,
Karen Zaruba
3M Company, St. Paul, MN
kszaruba1-at-mmm.com






From daemon Wed Nov 15 14:19:04 2000



From: S. Kuehner :      kuehner-at-u.washington.edu
Date: Wed, 15 Nov 2000 12:13:46 -0800 (PST)
Subject: beam overlaps

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hi-
We are analyzing experimental charges of magnetite + ilmenite in a
glass of rhyolitic composition. The problem we have run into is that the
ilmenite grains are very small and thin, typicaly {10um in length, such
that nearly all ilmenite analyses overlap the glass. My question is: Does
anyone have any experience/know-how/ideas that would help in calculating
the composition of the ilmenite from an analysis that is from a mixture of
ilmenite + glass.

Analyses are with a JEOL 733 microprobe at 15kV and 5nA. We have
not yet tried a lower kV that would potentially reduce the beam
penitration depth. Our method of recalculation thus far has been to
adjust the final analysis for density differences between glass and
ilmenite following the method of Warren (LPSC ?1997). The fraction of
glass is then subtracted from the analysis based on the amount of Sio2
(all SiO2 in the glass, no SiO2 in the ilmenite). We then adjust the
result by noting that the ZAF correction applied to the original analysis
is a function of the amount of glass overlap and extrapolate this
relationship for each element in ilmenite back to a ZAF at 0% Sio2.

TEM EDS is a last resort as the sample would have to be mined out
of a polished microprobe mount.

************************************************
### Free the Psoas ###
**************

Dr. Scott Kuehner kuehner-at-u.washington.edu
Dept. of Geological Sciences ph.206-543-8393
Box 351310 Fax 206-616-6873
The University of Washington
Seattle, Washington 98195-1310
************************************************

On Wed, 15 Nov 2000 kszaruba1-at-mmm.com-at-sparc5.microscopy.com wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
}
} Hello fellow microscopists,
}
} As part of a hazard review on our new ESEM, many questions were raised regarding
} the observation of biohazards. We won't be looking at actual human pathogens
} (although maybe others do?? CDC/NIH??). However, just looking at unfixed human
} skin puts us under the Class 2 category. Also we'll be observing non-pathogenic
} (opportunisitic) bacteria. Here are the questions:
}
} Are there conditions inside the ESEM that would favor the creation of an
} aerosol from the sample?
} Assuming any of the sample becomes detached/airborn inside the chamber, would
} pumping down to higher vacuum prior to opening the door remove them (seems
} logical that it would)?
} How do you dispose of pump oil containing microbes/biohazards? Would
} anything capable of infecting humans even survive in pump oil? (Remember
} we're talking about viruses as well as bacteria here).
} Finally, would the chamber walls, etc. need to be decontaminated
} periodically?
}
} If anyone has gone through this sort of safety review and generated a protocol,
} we'd love to benefit from your hard work!! Otherwise we'd appreciate any
} suggestions/opinions.
}
} Thanks as always,
} Karen Zaruba
} 3M Company, St. Paul, MN
} kszaruba1-at-mmm.com
}
}
}
}
}




From daemon Wed Nov 15 14:56:04 2000



From: Yuhui_Xu :      Yuhui_Xu-at-hms.harvard.edu
Date: Wed, 15 Nov 2000 15:50:03 -0500
Subject: Darkroom Paper printer, tissue processor etc.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Colleges:

We are in the market for buying a automated tissue processor for electron
microscopy and a paper printer for the dark room. I will appreciate any
recommendations for certain makes and models.

Thank you in advance for any help!
Regards,


Yuhui

Yuhui Xu, MD,PhD
EM Core, NHLBI, NIH




From daemon Thu Nov 16 03:30:05 2000



From: Gudrun.Hugelshofer-at-rdke.nestle.com
Date: Thu, 16 Nov 2000 10:19:17 +0100
Subject: LM-Protein stain in vapour form

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello,

Does anyone know if there is a protein stain you can apply in vapour form in
order not to change the structure of very delicat samples when using liquids
(as for e.g. starch and fat)?

Thanks for your help. Gudrun

Gudrun Hugelshofer
Nestlé Product Technology Centre Kemptthal, CH-8310 Kemptthal
Tel: ++41 (0)52 354 06 76 Fax: ++41 (0)52 354 07 14
E-mail: gudrun.hugelshofer-at-rdke.nestle.com






From daemon Thu Nov 16 06:27:14 2000



From: Frank Thomas :      thomasf-at-AGC.BIO.NS.CA
Date: Thu, 16 Nov 2000 08:21:36 -0400
Subject: Re: ESEM Biohazards?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We've had an ESEM for about 7 years now, but we've never looked at anything
remotely human in origin in it. However, I think I have a pretty good idea
of what conditions are really like in the chamber of an ESEM, so I'll
contribute my (fairly uneducated) guesses/impressions.
Here goes:

} Are there conditions inside the ESEM that would favor the creation of
an
aerosol from the sample?

Don't think so - unless your sample was easily evaporated...

Assuming any of the sample becomes detached/airborn inside the chamber,
would pumping down to higher vacuum prior to opening the door remove them
(seems logical that it would)?

You're right....that does seem logical...

} How do you dispose of pump oil containing microbes/biohazards? Would
} anything capable of infecting humans even survive in pump oil?
(Remember
} we're talking about viruses as well as bacteria here).

Being at an oceanographic institute, there are these really big blue vats
out on the dock, put there to receive waste oil from our ships. When I
change the oil in our rotary pumps, that's where it goes. I kind of doubt
that any human pathogens could live in pump oil (but that's a real
guess....)

} Finally, would the chamber walls, etc. need to be decontaminated
} periodically?
}
Probably not, but if it was deemed necessary, I suppose a rinse with some
kind of antiseptic wouldn't be a bad idea - as long as you kept the fluids
away from the stage electrics :-)

Good luck with the new ESEM - they're pretty nice, well-built instruments.

Frank Thomas
MicroAnalysis Facility
Geological Survey of Canada (Atlantic)
Bedford Institute of Oceanography
Dartmouth, Nova Scotia
Canada




From daemon Thu Nov 16 06:48:22 2000



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Thu, 16 Nov 2000 12:45:35 +0000 (GMT Standard Time)
Subject: Re: ESEM Biohazards?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


For safety reasons we fix human cell cultures and catheters
before examination in our ESEM. Unfixed human and animal
cells suffer beam damage in tungsten filament ESEMs so we
do not feel we are missing out. It would be nice to look
at the catheter biofilms unfixed but we plumped for a
safety first approach.

Dave


On Wed, 15 Nov 2000 13:26:22 -0600
"kszaruba1-at-mmm.com"-at-sparc5.microscopy.com wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
}
} Hello fellow microscopists,
}
} As part of a hazard review on our new ESEM, many questions were raised regarding
} the observation of biohazards. We won't be looking at actual human pathogens
} (although maybe others do?? CDC/NIH??). However, just looking at unfixed human
} skin puts us under the Class 2 category. Also we'll be observing non-pathogenic
} (opportunisitic) bacteria. Here are the questions:
}
} Are there conditions inside the ESEM that would favor the creation of an
} aerosol from the sample?
} Assuming any of the sample becomes detached/airborn inside the chamber, would
} pumping down to higher vacuum prior to opening the door remove them (seems
} logical that it would)?
} How do you dispose of pump oil containing microbes/biohazards? Would
} anything capable of infecting humans even survive in pump oil? (Remember
} we're talking about viruses as well as bacteria here).
} Finally, would the chamber walls, etc. need to be decontaminated
} periodically?
}
} If anyone has gone through this sort of safety review and generated a protocol,
} we'd love to benefit from your hard work!! Otherwise we'd appreciate any
} suggestions/opinions.
}
} Thanks as always,
} Karen Zaruba
} 3M Company, St. Paul, MN
} kszaruba1-at-mmm.com
}
}
}
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"




From daemon Thu Nov 16 08:12:45 2000



From: Gregory Mulhollan :      mulhollan-at-spec.com
Date: Thu, 16 Nov 2000 08:06:24 -0600
Subject: Diode array detector (Tracor Northern 6100)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Microscopy folks,
Anyone out there have any info on the Tracor-Northern 6100 diode array
detector as used in the TN spectrophotometers? Feel free to point me to
another list that is more appropriate for this question if you know of one.
Thanks,
Greg M.
--
Gregory Mulhollan, Ph.D.
Extreme Devices Inc.
101 West 6th Street
Suite 200
Austin, TX 78701
(512)479-7740 x2231 voice
(512)479-7750 fax
mulhollan-at-extremedevices.com




From daemon Thu Nov 16 08:47:03 2000



From: O. O. Ilori :      sojilori-at-oauife.edu.ng
Date: Thu, 16 Nov 2000 15:46:31 +0100 (WAT)
Subject: SEM training

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Guys,
I am looking for a place,an institute or something where I can be
trained to operate an SEM. Any suggestions?
Thanks.

Mr. O. O. ILORI
DEPARTMENT OF ELECTRONIC/ELECTRICAL ENGINEERING
OBAFEMI AWOLOWO UNIVERSITY,
ILE-IFE, OSUN STATE
NIGERIA.

email: sojilori-at-oauife.edu.ng





From daemon Thu Nov 16 09:59:41 2000



From: michael shaffer :      epmalab-at-darkwing.uoregon.edu
Date: Thu, 16 Nov 2000 07:54:22 -0800
Subject: RE: beam overlaps

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Scott writes ...

} We are analyzing experimental charges of magnetite +
} ilmenite in a glass of rhyolitic composition.
} The problem we have run into is that the
} ilmenite grains are very small ... My question is:
} Does anyone have any experience/know-how/ideas
} that would help in calculating the composition of the
} ilmenite from an analysis that is from a mixture of
} ilmenite + glass.
}
} ...

This will indeed be a problem ... not only do x-rays generated
outside the ilmente reach the detectors, but they also travel through
different compositions on their way to the spectrometers which are
located differently relative to the ilmenites orientation in the
glass. One spectro may measure Fe largely absorbed by the ilmenite,
and another may measure Ti largely absorbed by glass. You would have
to model each based on simplistic assumptions which may be
unjustified.

cheerios, =shAf= :o)

{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {}
Michael Shaffer, R.A. - mshaf-at-darkwing.uoregon.edu
Geological Science's Electron Probe Facility - University of Oregon
http://epmalab.uoregon.edu/





From daemon Thu Nov 16 10:40:23 2000



From: Greg Erdos :      gwe-at-biotech.ufl.edu
Date: Thu, 16 Nov 2000 11:37:09 -0500
Subject: Position Open

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


There is a position for an EM technician at the Electron Microscopy Core
lab of the Biotechnology Program at the University of Florida in
Gainesville. The laboratory is mostly a fee-for-service lab serving the
needs of biological, biomedical and agricultural scientists at the
university. Applicants must be skilled in the preparation of biological
samples for both scanning and transmission electron microscopy. Experience
with both PC and Mac as well as website management is
desirable. Experience with digital imaging and fluorescence microscopy
also a plus.
This is a full time, permanent position with standard benefits of State of
Florida employees.

For further details, reply to this message or contact Greg Erdos at the
number below.



Gregory W. Erdos, Ph.D.
Assistant Director, Biotechnology Program
PO Box 110580
University of Florida
Gainesville, FL 32611

Ph. 352-392-1295 Fax: 352-846-0251



From daemon Thu Nov 16 10:40:35 2000



From: William F. Tivol :      wft03-at-health.state.ny.us
Date: Thu, 16 Nov 2000 11:38:02 -0500
Subject: Re: LM-Protein stain in vapour form

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




} Does anyone know if there is a protein stain you can apply in vapour form in
} order not to change the structure of very delicat samples when using liquids
} (as for e.g. starch and fat)?

} Thanks for your help. Gudrun

Dear Gudrun,
I don't know what you intend to do, LM, EM, or other, but I once used a
stain to detect peptides on chromatography paper (it was a long time ago). 1)
Place the protein specimen in an enclosed box in a well-ventillated hood. 2)
Mix equimolar solutions of HCl and KMnO4 (in the hood, of course), place the
beaker containing the mix in the box alongside the specimen, and seal the box.
The Cl-atoms generated will react with the peptide bonds to produce
chloropeptides. 3) Add iodide (I added KI solution, but HI or ICl would
probably work.). Instead of the last step, you could add anything which reacts
with the chloropeptides and gives a signal which can be detected by whatever
type of microscopy you want to use. This stain is extremely sensitive, because
it can label each peptide bond, so a 100-residue protein can have 100 labels
attached. Good luck; please let me know if this technique works for you.
Yours,

Bill Tivol
Wadsworth Center
Albany NY
(518) 473-7399 WFT02-at-health.state.ny.us





From daemon Thu Nov 16 10:42:52 2000



From: William H Roberts :      William.H.Roberts-at-usa.dupont.com
Date: Thu, 16 Nov 2000 10:54:00 -0500
Subject: beam overlaps

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Scott,

I don't have any specific experience with the analytes and the matrix that
you reference, but have you tried using backscattered electron imaging?
There's a difference of a little over six in the average atomic number of
ilemite and magnetite with an even greater difference between the ilemite
and the volcanic glass matrix, so atomic number contrast should be no
problem. Most EDX systems offer routines for measuring phase area based on
atomic number contrast which is fairly easily translated into volume
concentration. You should have no problem with overlap using backscattered
electrons as opposed to x-rays. In addition, with the help of an image
analysis package, you can get size, shape, orientation and other
information at the same time. Just a thought.

Bill




"S. Kuehner" {kuehner-at-u.washington.edu} on 11/15/2000 03:13:46 PM

To: "kszaruba1-at-mmm.com"-at-sparc5.microscopy.com
cc: Microscopy-at-sparc5.microscopy.com (bcc: William H Roberts/US/FILM/DPT)


Hi-
We are analyzing experimental charges of magnetite + ilmenite in a
glass of rhyolitic composition. The problem we have run into is that the
ilmenite grains are very small and thin, typicaly {10um in length, such
that nearly all ilmenite analyses overlap the glass. My question is: Does
anyone have any experience/know-how/ideas that would help in calculating
the composition of the ilmenite from an analysis that is from a mixture of
ilmenite + glass.
Analyses are with a JEOL 733 microprobe at 15kV and 5nA. We have
not yet tried a lower kV that would potentially reduce the beam
penitration depth. Our method of recalculation thus far has been to
adjust the final analysis for density differences between glass and
ilmenite following the method of Warren (LPSC ?1997). The fraction of
glass is then subtracted from the analysis based on the amount of Sio2
(all SiO2 in the glass, no SiO2 in the ilmenite). We then adjust the
result by noting that the ZAF correction applied to the original analysis
is a function of the amount of glass overlap and extrapolate this
relationship for each element in ilmenite back to a ZAF at 0% Sio2.
TEM EDS is a last resort as the sample would have to be mined out
of a polished microprobe mount.
************************************************
### Free the Psoas ###
**************
Dr. Scott Kuehner kuehner-at-u.washington.edu
Dept. of Geological Sciences ph.206-543-8393
Box 351310 Fax 206-616-6873
The University of Washington
Seattle, Washington 98195-1310
************************************************






From daemon Thu Nov 16 10:46:00 2000



From: Lehman, Ann :      Ann.Lehman-at-trincoll.edu
Date: Thu, 16 Nov 2000 11:45:39 -0500
Subject: service for x-ray diffractometer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listfolk,

This is slightly off the EM mark, but still in the ballpark for this List...

We recently received a donated xray diffractometer for which we need service
and installation assistance. Does anyone know of a local rep or contact
info??

It is a Philips APD 3720 Automated Power Diffraction System with a Philips
XRG 3100 X-Ray Generator, vintage ca. mid-80's.

Thanks, all.

Ann Hein Lehman

----------------
Ann Hein Lehman
EM Facility Manager
Trinity College
Hartford, CT 06106
v. 860-297-4289
f. 860-297-2538
e. ann.lehman-at-trincoll.edu
w. http://www.trincoll.edu/~alehman



From daemon Thu Nov 16 11:12:19 2000



From: William McManus :      billemac-at-biology.usu.edu
Date: Thu, 16 Nov 2000 10:08:04 -0700
Subject: SEM training

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I realize that you are pretty far away, but we offer an electron
microscopy course here at USU, each spring semester, which includes a
large segment on SEM.

William McManus
Supervisor
Electron Microscopy Facility
Department of Biology
Utah State University
Logan UT 84322-5305

billEMac-at-cc.usu.edu
435-797-1920


-----Original Message-----
} From: O. O. Ilori [mailto:sojilori-at-oauife.edu.ng]
Sent: Thursday, November 16, 2000 7:47 AM
To: microscopy-at-sparc5.microscopy.com


Hello Guys,
I am looking for a place,an institute or something where I can be
trained to operate an SEM. Any suggestions?
Thanks.

Mr. O. O. ILORI
DEPARTMENT OF ELECTRONIC/ELECTRICAL ENGINEERING
OBAFEMI AWOLOWO UNIVERSITY,
ILE-IFE, OSUN STATE
NIGERIA.

email: sojilori-at-oauife.edu.ng





From daemon Thu Nov 16 12:46:14 2000



From: William H Roberts :      William.H.Roberts-at-usa.dupont.com
Date: Thu, 16 Nov 2000 13:12:14 -0500
Subject: beam overlaps

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Scott,

I don't have any specific experience with the analytes and the matrix that
you reference, but have you tried using backscattered electron imaging?
There's a difference of a little over eleven in the average atomic number
of ilmenite and magnetite with an even greater difference between the
ilmenite and the volcanic glass matrix, so atomic number contrast should be
no problem. Most EDX systems offer routines for measuring phase area based
on atomic number contrast which is fairly easily translated into volume
concentration. You should have no problem with overlap using backscattered
electrons as opposed to x-rays. In addition, with the help of an image
analysis package, you can get size, shape, orientation and other
information at the same time. Just a thought.

Bill




"S. Kuehner" {kuehner-at-u.washington.edu} on 11/15/2000 03:13:46 PM

To: "kszaruba1-at-mmm.com"-at-sparc5.microscopy.com
cc: Microscopy-at-sparc5.microscopy.com (bcc: William H Roberts/US/FILM/DPT)


Hi-
We are analyzing experimental charges of magnetite + ilmenite in a
glass of rhyolitic composition. The problem we have run into is that the
ilmenite grains are very small and thin, typicaly {10um in length, such
that nearly all ilmenite analyses overlap the glass. My question is: Does
anyone have any experience/know-how/ideas that would help in calculating
the composition of the ilmenite from an analysis that is from a mixture of
ilmenite + glass.
Analyses are with a JEOL 733 microprobe at 15kV and 5nA. We have
not yet tried a lower kV that would potentially reduce the beam
penitration depth. Our method of recalculation thus far has been to
adjust the final analysis for density differences between glass and
ilmenite following the method of Warren (LPSC ?1997). The fraction of
glass is then subtracted from the analysis based on the amount of Sio2
(all SiO2 in the glass, no SiO2 in the ilmenite). We then adjust the
result by noting that the ZAF correction applied to the original analysis
is a function of the amount of glass overlap and extrapolate this
relationship for each element in ilmenite back to a ZAF at 0% Sio2.
TEM EDS is a last resort as the sample would have to be mined out
of a polished microprobe mount.
************************************************
### Free the Psoas ###
**************
Dr. Scott Kuehner kuehner-at-u.washington.edu
Dept. of Geological Sciences ph.206-543-8393
Box 351310 Fax 206-616-6873
The University of Washington
Seattle, Washington 98195-1310
************************************************






From daemon Thu Nov 16 13:33:01 2000



From: Dorothy Roak Sorenson :      dsoren-at-umich.edu
Date: Thu, 16 Nov 2000 14:30:18 -0500 (EST)
Subject: collagen stain for Epon embedded tissue

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello all,

Does anyone know of a way to stain for collagen in Epon embedded cardiac
muscle for viewing by light microscopy?

Thanks for any suggestions.

Dotty

Dotty Sorenson
Microscopy and Image Analysis Laboratory
Department of Cell and Developmental Biology
University of Michigan Medical School
Ann Arbor, Michigan
dsoren-at-umich.edu
(734)763-1170




From daemon Thu Nov 16 14:21:42 2000



From: kszaruba1-at-mmm.com
Date: Thu, 16 Nov 2000 14:16:34 -0600
Subject: Re: collagen stain for Epon embedded tissue

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Dotty,

Of course immunolabeling might work. I've had success with collagen types III
and IV in Epon and Spurr's resin, definitely not processed with immuno in mind.

On the other hand if you just want something simple to help highlight the
collagen a little, I've seen Methyl Green staining collagen more than other
components in skin cross sections.

Good luck,
Karen

---------------------------------------
Hello all,

Does anyone know of a way to stain for collagen in Epon embedded cardiac
muscle for viewing by light microscopy?

Thanks for any suggestions.

Dotty

Dotty Sorenson
Microscopy and Image Analysis Laboratory
Department of Cell and Developmental Biology
University of Michigan Medical School
Ann Arbor, Michigan
dsoren-at-umich.edu
(734)763-1170





From daemon Thu Nov 16 15:44:23 2000



From: Lucille A. Giannuzzi :      lag-at-mail.ucf.edu
Date: Thu, 16 Nov 2000 16:35:28 -0500
Subject: FIB'ing and more in FL in March

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Come join us at the joint meetings of Florida Microscopy Society, FL AVS,
and Surface Science 2001

March 12-16, 2001

University of Central Florida, Orlando, FL

THERE IS NO REGISTRATION FEE TO ATTEND THE MEETING (but please register at
http://natasha.eng.usf.edu/gilbert/avs/anouncement/registrationform.html so
we get an accurate head count for meals)

- An FIB afternoon session followed by:

- An early evenning session devoted to an FIB workshop and users group
meeting (open to all users of FIBs and all FIB manufacturers)

- There will be 2 FIB-related short courses
one on theory (see www.vacuum.org for fees and information)
FIB will be included in a comprehensive TEM specimen preparation
course March 14-16, 2001
(contact lag-at-mail.ucf.edu)

To submit an abstract for the meeting or for more information follow the FL
local affiliates link to FL from: www.microscopy.org

or contact Lucille Giannuzzi, lag-at-mail.ucf.edu


*******************************************************************
Lucille A. Giannuzzi, Ph.D.

Associate Professor, Mechanical, Materials, and Aerospace Engineering
Director, UCF/Cirent Materials Characterization Facility
University of Central Florida
12443 Research Parkway, Suite 305
Orlando, FL 32826 email lag-at-mail.ucf.edu phone (407) 275-4354,5,6
fax (407) 275-4321
--------------------------------------------------------------------
"Good judgement comes from experience.

Experience comes from making bad judgement."

Mark Twain
********************************************************************





From daemon Thu Nov 16 15:47:58 2000



From: William H Roberts :      William.H.Roberts-at-usa.dupont.com
Date: Thu, 16 Nov 2000 16:37:55 -0500
Subject: SVMicro Digital Microscope Camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers,

Does anyone have an experience that they would like to share involving the
SVMicro digital camera by Sound Vision? I'm hoping to learn anything at
all, from delivery, support, service, image quality, product reliability,
etc. Thanks in advance.

Bill Roberts





From daemon Thu Nov 16 17:13:09 2000



From: Walck, Scott D. :      walck-at-ppg.com
Date: Thu, 16 Nov 2000 18:07:20 -0500
Subject: RE: SVMicro Digital Microscope Camera anybody want to trade?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have one. It is about two years old. It can provide good images, but it
is tough to work with and you have to work too hard to get a good image.
The TWAIN software that I have is tough to set up the contrast, brightness,
and exposure. The person to whom I gave this camera didn't like it, gave up
and went back to film. I then traded him my Pixera camera for the SVMicro
and he was much happier. I don't know if the software on the SVMicro has
improved or not.

We don't do really critical work with either microscope that these are put
on and we usually don't do color.

I would trade the SVMicro for another El Cheapo Pixera for the ease of use.



Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)


"The opinions expressed are those of Scott D. Walck and not of PPG
Industries, Inc. nor of any PPG-associated companies."
--

-----Original Message-----
From: William H Roberts [mailto:William.H.Roberts-at-usa.dupont.com]
Sent: Thursday, November 16, 2000 4:38 PM
To: Microscopy-at-sparc5.microscopy.com
Subject: SVMicro Digital Microscope Camera


---------------------------------------------------------------
---------
The Microscopy ListServer -- Sponsor: The Microscopy Society
of America
To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
On-Line Help
http://www.msa.microscopy.com/MicroscopyListserv er/FAQ.html



---------------------------------------------------------------
--------.


Dear Listers,

Does anyone have an experience that they would like to share
involving the
SVMicro digital camera by Sound Vision? I'm hoping to learn
anything at
all, from delivery, support, service, image quality, product
reliability,
etc. Thanks in advance.

Bill Roberts






From daemon Thu Nov 16 19:50:55 2000



From: Ray D. Twesten :      twesten-at-uiuc.edu
Date: Thu, 16 Nov 2000 19:39:38 -0600
Subject: RE: SVMicro Digital Microscope Camera anybody want to trade?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have a SVMicro attached to an old Zeiss Ultraphot. We do not do anything
critical with it; just BF/DF and DIC at medium mags on materials samples. I
would say it works quite well. We use the Photoshop interface for the Mac;
the interface is quite easy to work with. One caveat, the sensor is quite
large (~25mm) so you need good transfer optics to get a good image over the
whole sensor.
At the time (~2yrs bp), the performance to price ratio was very high. The
whole package was around $1100. I do not know how it compares now.
Just my $0.02.


Ray D. Twesten, PhD.
Center for Microanalysis of Materials
Seitz Materials Research Laboratory
104 S. Goodwin Ave., Urbana, IL 61801
+1 217 244-6177 (Fax -2278)


-----Original Message-----
} From: Walck, Scott D. [mailto:walck-at-ppg.com]
Sent: Thursday, November 16, 2000 5:07 PM
To: 'William H Roberts'
Cc: 'Microscopy'


We have one. It is about two years old. It can provide good images, but it
is tough to work with and you have to work too hard to get a good image.
The TWAIN software that I have is tough to set up the contrast, brightness,
and exposure. The person to whom I gave this camera didn't like it, gave up
and went back to film. I then traded him my Pixera camera for the SVMicro
and he was much happier. I don't know if the software on the SVMicro has
improved or not.

We don't do really critical work with either microscope that these are put
on and we usually don't do color.

I would trade the SVMicro for another El Cheapo Pixera for the ease of use.



Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)


"The opinions expressed are those of Scott D. Walck and not of PPG
Industries, Inc. nor of any PPG-associated companies."
--

-----Original Message-----
From: William H Roberts [mailto:William.H.Roberts-at-usa.dupont.com]
Sent: Thursday, November 16, 2000 4:38 PM
To: Microscopy-at-sparc5.microscopy.com
Subject: SVMicro Digital Microscope Camera


---------------------------------------------------------------
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Dear Listers,

Does anyone have an experience that they would like to share
involving the
SVMicro digital camera by Sound Vision? I'm hoping to learn
anything at
all, from delivery, support, service, image quality, product
reliability,
etc. Thanks in advance.

Bill Roberts







From daemon Fri Nov 17 09:17:50 2000



From: beth-at-dogwood.botany.uga.edu (Beth Richardson)
Date: Fri, 17 Nov 2000 10:09:42 -0500
Subject: Casio digital camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,
We are looking for opinions or reviews on the the Casio QV 3000 digital
camera, with a 340 MB microdrive and macro focusing down to 6 cm.
any help would be greatly appreciated.
thanks,
Beth

**************************************
Beth Richardson
EM Lab Coordinator
Botany Department
University of Georgia
Athens, GA 30602

Phone - (706) 542-1790
FAX - (706) 542-1805
Email - beth-at-dogwood.botany.uga.edu

"Between the two evils,
I always pick the one I never tried before". Mae West (1893-1980)
**************************************





From daemon Fri Nov 17 09:52:26 2000



From: Marek Malecki :      mmm-at-ucsd.edu
Date: Fri, 17 Nov 2000 00:19:10 -0800
Subject: Automatic sample processors for EM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I would appreciate comments and suggestions on automatic sample processors
for EM. Vendors welcome.
Marek Malecki, M.D., Ph.D.
Director and Principal Investigator

Molecular Imaging Laboratories
University of California, San Diego

address: 1500 Suite Bonner Hall, 9500 Gilman Drive, La Jolla, CA92093-0368
phone - office: 8588223373
phone - cell: 8583443347
fax: 8588223715
e.mail: mmm-at-ucsd.edu
pager: 8586161420
e.mail: mmm-at-ucsd.edu
e.pager: 1620024619-at-alphapage.airtouch.com
www site: http://mil.ucsd.edu/
ftp site: mil1.ucsd.edu





From daemon Fri Nov 17 11:10:14 2000



From: Awbrey, Donald :      DonaldAwbrey-at-texashealth.org
Date: Fri, 17 Nov 2000 10:27:29 -0600
Subject: TEM stains

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Dear TEM netters,

Is there any other stain reagents, other than UA and LC, that are
used in TEM? If so, do they have comparable results, are they
ETOH or water based, what are there disposal precautions, and what
are their staining protocols.

Any information will be appreciated.

Thanks in advance.



Donald G. Awbrey, HT(ASCP) QIHC
Electron Microscopy / Image Analysis
DonaldAwbrey-at-TexasHealth.org
donaldawbrey-at-hotmail.com
(817)-878-5647





From daemon Fri Nov 17 12:57:30 2000



From: Della Miller :      della-at-vacuum.org
Date: Fri, 17 Nov 2000 11:07:40 -0600
Subject: AVS/Microscopy Joint Meeting

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

I am in the process of publishing a brochure that advertises the AVS short
courses and symposium in Florida. Since this is joint meeting would it be
possible to rent/exchange a mailing list from the Society for this purpose.
The meeting is March 2001.

Della

_____________________________

Della Miller
AVS West
1265 El Camino Real, Ste. 109
Santa Clara CA 95050

Phone: 408-246-3600
Fax: 408-246-7700
E-mail: della-at-vacuum.org
Web: www.vacuum.org




From daemon Fri Nov 17 13:29:16 2000



From: Dunlap, Jonathan C. :      Jonathan.Dunlap-at-sylvania.com
Date: Fri, 17 Nov 2000 14:24:30 -0500
Subject: Edwards E306A

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello All:

I have walked into a laboratory that has had an Edwards E306A Vacuum Coater
purchased in 1991. It has been used very rarely since, and by no one
presently working in the laboratory. The instruction manuals help to pump
down the system and get it ready for the application, but I have yet to get
the carbon source deposited on the sample we are trying to coat for SEM
analysis (I can get the carbon rods glowing, but nothing else happens).

I am wondering if anyone out there has this system and knows how to operate
it with the carbon evaporation source. Any help to what I could be doing
wrong, or not doing at all would be appreciated. Also perhaps if I could
call someone who has this system and they could walk me though the steps
would be great.

- Jonathan

(I have contacted the manufacturer and they have not made this coater in
years so know little about it as well)



From daemon Fri Nov 17 13:59:00 2000



From: Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 17 Nov 2000 11:36:15 -0800
Subject: Re: Casio digital camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I would recommend that you check out

www.dejanews.com

and do an ALL groups, all history search for "casio 3000."
I did this and found 800 hits. Most are as expected
in the rec.photo.digital usenet forum. I did not
immediately see any threads regarding its use on
a microscope. So, this may or may not be of
value to you.

gary g.


At 07:09 AM 11/17/00, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From daemon Fri Nov 17 14:49:58 2000



From: Leona Cohen-Gould :      lcgould-at-mail.med.cornell.edu
Date: Fri, 17 Nov 2000 15:46:52 -0500
Subject: Re: TEM stains

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 10:27 AM -0600 11/17/00, Awbrey, Donald wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_______________________________________________________

Dear Donald,

Yes there are other TEM stains, although UrAc and Lead Citrate are by far
the most commonly used. You can also use Bismuth in lieu of lead. Kits
are commercially available and come with instructions. Its a similar
protocol to that used for Pb, but with concetration and time differences.
For a comprehensive review, you can refer to Vol. 5 Part I: Staining
Methods for Sectioned Material. by PR Lewis and DP Knight in the
"Practical Methods on Electron Microscopy series edited by Audrey Glauert.
This covers general as well as cytochemical methods.

Lee


Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175





From daemon Fri Nov 17 20:38:15 2000



From: christopher :      egon-at-eclipse.net
Date: Fri, 17 Nov 2000 21:45:24 -0500
Subject: LKB Ultamicrotome III problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I came across a used Blazers Electon Beam Evaroration unit with a 552 gun
BAF 300 Freeze Etch unit BAE 300 Coating unit 300, Crystal thin film
monitor QSG 202, dual rotary vane vacuum pumps and what appears to be all
documentation. .

The unit was removed from a working lab but it was not decommissioned by
experienced professionals. It did appear to be carefully taken down. I
think it was taken for a decommissioned lab because many their notes on
procedures are included

It is for sale by a friend of mine who I won't plug on the list. But it
looks like it might be a good deal for some one that need a set up like
this and he is not very proud of it. I don't normally do this but I hate
to see something that appears to be this good go for scrap. Even though I
would like to have the vacuum chamber.

Gordon
Gordon Couger gcouger-at-couger.com
Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00




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Good Evening, all:

We have an old LKB Ultramicrotome III (series 8800) that has developed a
problem in the past few weeks. Apparently the cantilever arm is not going
down far enough on the cutting stroke to activate the kickback on the knife
holder, resulting in wet blocks nearly every stroke. Needless to say, this
is a huge pain in the butt. We're kind of fond of the old machine, so we're
interested in fixing it.
My question is: does anyone know if there is an adjustment to change at
what point the relay for the knife holder kicks in? Or, is there some way
to get the arm to travel farther down?
Thanks much!


-Chris

(Sorry....I don't have a fancy title yet...I'm still just a student!)




From daemon Sun Nov 19 06:23:00 2000



From: oftzi-at-Ladymail.cz
Date: Sat, 18 Nov 2000 15:14:08 -0400
Subject: Tired of the 40 X 40 Plan? 26724

Contents Retrieved from Microscopy Listserver Archives
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Tired of the 40 X 40 X 40 Plan? You know:

Work 40 hours per week for someone else for 40 years, then receive a 40% reduction in pay!

Is working for a "boss" too demeaning and unrewarding?

Are you sick of depending on a job with too little pay and too many hours with no personal reward and even less future?

If you're determined to retire in the next 2 - 5 years with enough income to have REAL Financial Independence and Freedom, and are not afraid to work for it, I can help you.

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YOUR FINANCIAL PAST DOES NOT HAVE TO BE YOUR FINANCIAL FUTURE!

CALL ONLY IF YOU ARE SERIOUS!

1-800-533-9350 (Free, 24 hour, 2 minute recorded message)

DON'T GO TO SLEEP WITHOUT LISTENING TO THIS!

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To be removed from future mailings, send an email to: qwertyax5-at-yahoo.com and type "Remove" in the subject line.



From daemon Sun Nov 19 11:12:22 2000



From: Gregory Mulhollan :      mulhollan-at-spec.com
Date: Sun, 19 Nov 2000 11:02:39 -0600
Subject: Diode array detector (Tracor Northern 6100)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi folks,
Anyone out there have any info on the Tracor-Northern 6100 diode array
detector as used in the TN spectrophotometers? Feel free to point me to
another list that is more appropriate for this question if you know of one.
Thanks,
Greg M.
--
Gregory Mulhollan, Ph.D.
Extreme Devices Inc.
101 West 6th Street
Suite 200
Austin, TX 78701
(512)479-7740 x2231 voice
(512)479-7750 fax
mulhollan-at-extremedevices.com





From daemon Sun Nov 19 11:16:13 2000



From: Lou Solebello :      microls1297-at-mindspring.com
Date: Sun, 19 Nov 2000 11:08:05 -0600
Subject: Microscopists in the state of Virginia

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


For microscopists in the state of Virginia. Please contact Lou
Solebello at microls1297-at-mindspring.com if you are interested in getting
involved, or would attend regular meetings held in Richmond. I would like
to start up a Virginia Microscopical Society if there is enough interest
to do so. Professionals, hobbyists, teachers all welcome. Let me know if
you are interested, and what types of activities and presentations you
would like to see. Lou Solebello





From daemon Sun Nov 19 11:16:16 2000



From: Missy Josephson :      josepem-at-vetmed.auburn.edu
Date: Sun, 19 Nov 2000 11:08:25 -0600
Subject: Leitz objectives

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear listers,
I've inherited an old (1970s?) Leitz Orthoplan microscope in great need of
a cleaning and possibly some new objectives. Can anyone give advice on who
can help me rehabilitate it? I'm in the Atlanta-Birmingham-Montgomery
(Alabama) area.
Thanks
Missy Josephson

Eleanor M. Josephson, D.V.M., Ph.D.
Assistant Professor
Department of Anatomy, Physiology and Pharmacology
College of Veterinary Medicine
111 Greene Hall
Auburn University, AL 36849-5518
Ph. (334) 844-5423
FAX (334) 844-4542
josepem-at-vetmed.auburn.edu





From daemon Mon Nov 20 00:36:20 2000



From: Robin Cross :      r.cross-at-ru.ac.za
Date: Mon, 20 Nov 2000 08:26:41 +0200
Subject: Re: LKB Ultamicrotome III problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Chris

} We have an old LKB Ultramicrotome III (series 8800) that has
} developed a problem in the past few weeks.
} My question is: does anyone know if there is an adjustment to change
} at what point the relay for the knife holder kicks in?

Yes, there is and I have the relevant LKB service note somewhere.
I'll be happy to fax it to you if you email me a fax number that will
reach you.

Regards

Rob


Robin H Cross
Director : EM Unit, Rhodes University, Grahamstown, South Africa
R.Cross-at-ru.ac.za
tel: +27 46 603 8168 - fax: +27 46 622 4377
http://www.ru.ac.za/emu/index.htm

** remember that ICEM-15 in 2002 is in Durban, South Africa **



From daemon Mon Nov 20 03:19:23 2000



From: Erdem Yasar :      yasar-at-turkuaz.kku.edu.tr
Date: Mon, 20 Nov 2000 11:08:28 +0200 (EET)
Subject: ABOUT JSM5600 SEM AND JEM3010 TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Sir/Madam ,
JEM3010 was manufactured in 1999.it has got 1.500.000x mag and digital
camera system JSM5600 has got 300.000xmag and EDS system.
we have two kind of chose for prices. If we study together (I mean we
preapare a paper together), we reseach samples without any price. On the
other hand we get approximately 30$ per each sample. But if you have many
samples we can make a discount.
Thanks for your interests..
I am waiting for your emails

**********************************
**********************************
** Research.Asst.ERDEM YASAR **
** University of Kirikkale **
** Department of Physics **
** TEM LABORATORY **
** 71450 KIRIKKALE/TURKEY **
** erdem.yasar-at-physics.org **
** yasar-at-science.ankara.edu.tr **
** yasar-at-turkuaz.kku.edu.tr **
**********************************
**********************************









From daemon Mon Nov 20 03:19:23 2000



From: Per =?iso-8859-1?Q?Hörstedt?= :      per.horstedt-at-pathol.umu.se
Date: Mon, 20 Nov 2000 10:09:43 +0100
Subject: Re: Casio digital camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Beth,
I'm using the Casio QV 3000 privately and I'm very satisfied with it. High
resolution, light sensitive CCD, easy to use functions and easily
understandable menus. Nearly all my images are correctly exposed, and the
macro setting works great, with really high resolution. It uses standard
batteries, or as I do rechargeables, that can be bought everywhere.
The camera body is slightly larger than most of the competitors, but this
makes it so much easier to hold with a steady grip.
All this together with the possibility of storing 244 images at high
resolution with the 340 mB drive makes it one of the best choices. The
Photoloader software package is easy to work with and makes the handling
of your images in the PC fast and well arranged. I'm using a Lexar card
reader, and image transfer to my PC is really fast, although USB usage is
even faster.
The only disadvantage I´ve found is that the software does not save images
in TIFF- format, only JPEG.
It's a good alternative to my Hasselblad and a lot easier to carry around
outdoors.
Now, you did not state what you need it for, ordinary photography is as you
can see OK, but I think it would be hard to get adaptors to use it, for
example, as a microscope camera .




Yours sincerely

Per Hörstedt
Department of Medical Biosciences
Pathology
Unit for Electron Microscopy
University of Umeå
S-90187 Umeå
Sweden

phone int-46-90-7851541
fax int-46-90-7851215




From daemon Mon Nov 20 03:43:09 2000



From: David Vowles :      djv23-at-msm.cam.ac.uk
Date: Mon, 20 Nov 2000 09:37:38 +0000 (GMT)
Subject: Re: Edwards E306A

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Jonathan,

We have two Edwards 306 coaters in our lab. As with all carbon rod based
evaporators you will need to experiment with the spring pressure holding
the rods together. Too much pressure and the rods will glow and not arc
properly (most likely the situation you described) or, too little pressure
and the arc will cease prematurely.
On the 306 coaters the spring pressure is adjusted by lengthening or
shortening the carbon rods - first loosen the clamping collars and slide
the rods through.

Hope this is some help.

David Vowles
Electron Microscope Unit
Dept of Materials Science and Metallurgy
University of Cambridge
Pembroke St Cambridge
UK CB2 3QZ
Tel: +44 1223 334325
Fax: +44 1223 334567
Email: djv23-at-msm.cam.ac.uk

On Fri, 17 Nov 2000, Dunlap, Jonathan C. wrote:

} I have walked into a laboratory that has had an Edwards E306A Vacuum Coater
} purchased in 1991. It has been used very rarely since, and by no one
} presently working in the laboratory. The instruction manuals help to pump
} down the system and get it ready for the application, but I have yet to get
} the carbon source deposited on the sample we are trying to coat for SEM
} analysis (I can get the carbon rods glowing, but nothing else happens).




From daemon Mon Nov 20 03:45:38 2000



From: Malcolm Haswell :      malcolm.haswell-at-sunderland.ac.uk
Date: Mon, 20 Nov 2000 09:32:38 +0000
Subject: Re: LKB Ultamicrotome III problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Christopher

there are two possibilities that spring to mind:
1. you've probably checked this but if the cutting range is set too high the arm
might not fall far enough to engage the retract mechanism.
2. something in the retract circuit may have blown. It happened once on an LKBI and
the engineer found a blown capacitor. I assume that there must be a fairly big
current activating the electromagnet.

Have you watched the cutting cycle through the binoculars? Does it appear to try to
move at the bottom of the cutting stroke or make a clicking noise? How far below the
mid knife position does the specimen arm travel (it should be several millimetres)?

Good luck

Malcolm Haswell
e.m. unit
University of Sunderland
UK

christopher wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Good Evening, all:
}
} We have an old LKB Ultramicrotome III (series 8800) that has developed a
} problem in the past few weeks. Apparently the cantilever arm is not going
} down far enough on the cutting stroke to activate the kickback on the knife
} holder, resulting in wet blocks nearly every stroke. Needless to say, this
} is a huge pain in the butt. We're kind of fond of the old machine, so we're
} interested in fixing it.
} My question is: does anyone know if there is an adjustment to change at
} what point the relay for the knife holder kicks in? Or, is there some way
} to get the arm to travel farther down?
} Thanks much!
}
} -Chris
}
} (Sorry....I don't have a fancy title yet...I'm still just a student!)




From daemon Mon Nov 20 05:59:24 2000



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Mon, 20 Nov 2000 11:52:18 +0000 (GMT Standard Time)
Subject: Re: TEM stains

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We used to use 1%KMNO4 in phosphate buffer, pH 6.5 instead
of UA. We stained for 5min. It is supposed to be good for
membranes. We flushed it down the sink.

Dave


On Fri, 17 Nov 2000 10:27:29 -0600 "Awbrey, Donald"
{DonaldAwbrey-at-texashealth.org} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
}
} Dear TEM netters,
}
} Is there any other stain reagents, other than UA and LC, that are
} used in TEM? If so, do they have comparable results, are they
} ETOH or water based, what are there disposal precautions, and what
} are their staining protocols.
}
} Any information will be appreciated.
}
} Thanks in advance.
}
}
}
} Donald G. Awbrey, HT(ASCP) QIHC
} Electron Microscopy / Image Analysis
} DonaldAwbrey-at-TexasHealth.org
} donaldawbrey-at-hotmail.com
} (817)-878-5647
}
}
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"




From daemon Mon Nov 20 07:20:17 2000



From: Roger Moretz :      rcmoretz-at-excite.com
Date: Mon, 20 Nov 2000 04:59:53 -0800 (PST)
Subject: Re: Automatic sample processors for EM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Mareck:

I have responded to these queries before, so here goes again. The unit I
have used for years is the Lynx Tissue Processor (currently available from
EM Sciences). The original unit I purchased (from the manufacturer) was
marvelous. A second unit (purchased when Leica was marketing it) took over
5 years to get it into reliable working condition. My guess is that EMS
will provide a reliable product. The only other unit I know of is sold by
RMC (recently cycled through Ventana and now Boekeler -- Tucson, AZ) that is
an updated version of the old LKB design. I know of very happy users for
this product, but I honestly do not know the current status under the new
owners. I have attempted to get a demo of the RMC device, but RMC never
followed through, and the last incarnations have occurred so quickly that I
still can't provide a personal comparison.

Roger Moretz, Ph.D.
Dept of Toxicology
Boehringer Ingelheim Pharmaceuticals, Inc.
On Fri, 17 Nov 2000 00:19:10 -0800, Marek Malecki wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I would appreciate comments and suggestions on automatic sample
processors
} for EM. Vendors welcome.
} Marek Malecki, M.D., Ph.D.
} Director and Principal Investigator
}
} Molecular Imaging Laboratories
} University of California, San Diego
}
} address: 1500 Suite Bonner Hall, 9500 Gilman Drive, La Jolla,
CA92093-0368
} phone - office: 8588223373
} phone - cell: 8583443347
} fax: 8588223715
} e.mail: mmm-at-ucsd.edu
} pager: 8586161420
} e.mail: mmm-at-ucsd.edu
} e.pager: 1620024619-at-alphapage.airtouch.com
} www site: http://mil.ucsd.edu/
} ftp site: mil1.ucsd.edu
}
}
}





_______________________________________________________
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From daemon Mon Nov 20 07:25:41 2000



From: Roger Moretz :      rcmoretz-at-excite.com
Date: Mon, 20 Nov 2000 05:12:11 -0800 (PST)
Subject: Re: TEM stains

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Donald:

In addition to the Glauert series, there are the Hayat books (one
specifically on stains, and volume one of the EM series -- both may be out
of print now, but they are all available through interlibrary loan) and any
of the basic biological TEM books also should cover (at least mentioning)
stains other than UrAc and Pb Citrate. Bismuth is the only one I have used,
and it is really a bear to work with. Potassium permanganate has also been
used as have lanthanum, phosphotungstic acid and ruthenium. The Hayat book
is "Stains and Cytochemical Methods", Plenum Press, 1993. The other one is
"Principles and Techniques of Electron Microscopy; Biological Appications,
Volume I". Wiley has a huge volume on EM methods (it is a loose-leaf beast
that comes with updates--or it used to) that includes all of these methods
as well.

Roger Moretz, Ph.D.
Dept of Toxicology
Boehringer Ingelheim Pharmaceuticals, Inc.
On Fri, 17 Nov 2000 10:27:29 -0600, Awbrey, Donald wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
}
} Dear TEM netters,
}
} Is there any other stain reagents, other than UA and LC, that are
} used in TEM? If so, do they have comparable results, are they
} ETOH or water based, what are there disposal precautions, and what
} are their staining protocols.
}
} Any information will be appreciated.
}
} Thanks in advance.
}
}
}
} Donald G. Awbrey, HT(ASCP) QIHC
} Electron Microscopy / Image Analysis
} DonaldAwbrey-at-TexasHealth.org
} donaldawbrey-at-hotmail.com
} (817)-878-5647
}
}
}





_______________________________________________________
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From daemon Mon Nov 20 07:34:46 2000



From: Roger Moretz :      rcmoretz-at-excite.com
Date: Mon, 20 Nov 2000 05:26:29 -0800 (PST)
Subject: Re: Edwards E306A

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Jonathan:
Don't feel all alone. I purchased a 306A about 16 years ago when Edwards
was manufacturing and marketing the units. Even then, they didn't seem to
know all that much about the units. I had problems with the main valve, and
got less than no help. I don't think I EVER got a decent coat of carbon out
of the unit. I did manage to melt down a set of carbon rod holders and
burned out a transformer, but it never did provide a decent carbon coat. It
did provide a good metal (read, gold, for SEM) coat, and I had purchased it
because of access to electrodes and the great rotary table provided with it.
However, I eventually went back to the old reliable Denton for everything
but the gold coating, and then managed to get a sputter coater, so the 306A
was left standing, abandoned. Justice at last.

Roger Moretz, Ph.D.
Dept of Toxicology
Boehringer Ingelheim Pharmaceuticals, Inc.
On Fri, 17 Nov 2000 14:24:30 -0500, Dunlap, Jonathan C. wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hello All:
}
} I have walked into a laboratory that has had an Edwards E306A Vacuum
Coater
} purchased in 1991. It has been used very rarely since, and by no one
} presently working in the laboratory. The instruction manuals help to
pump
} down the system and get it ready for the application, but I have yet to
get
} the carbon source deposited on the sample we are trying to coat for SEM
} analysis (I can get the carbon rods glowing, but nothing else happens).
}
} I am wondering if anyone out there has this system and knows how to
operate
} it with the carbon evaporation source. Any help to what I could be doing
} wrong, or not doing at all would be appreciated. Also perhaps if I could
} call someone who has this system and they could walk me though the steps
} would be great.
}
} - Jonathan
}
} (I have contacted the manufacturer and they have not made this coater in
} years so know little about it as well)
}





_______________________________________________________
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From daemon Mon Nov 20 07:51:21 2000



From: Gilles Grondin :      ggrond01-at-courrier.usherb.ca
Date: Mon, 20 Nov 2000 08:39:26 -0500
Subject: Mitochondrial antibodies..

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We are looking for antibodies to mitochondrial proteins , for a
immunocytochemical study on the effect of parasites in the development of
youngs birds . Does anyone or company have mitochondrial antibodies ?

Thank you very much .

Gilles Grondin

ggrond01-at-courrier.usherb.ca




From daemon Mon Nov 20 08:35:19 2000



From: Al Coritz :      al-at-boeckeler.com
Date: Fri, 17 Nov 2000 16:59:42 -0700
Subject: Casio digital camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All:

Beth...I have owned the Casio QV-3000 since April 2000. I think it a
totally awesome for Macro work. A picture is worth a 1000 words...please go
to my online photo album and look for yourself. Some of the pictures have a
reference scale in them.

http://communities.msn.com/CactusCritters

Best,

Al Coritz
Sales & Service Manager
RMC-Boeckeler Instruments
4650 S. Butterfield Dr.
Tucson, AZ 85714
Voice: 520-745-0001
Cell: 520-465-3598
Fax: 520-745-0004
Email:Al-at-Boeckeler.com
Website:RMCProducts.com


-----Original Message-----
} From: Beth Richardson [mailto:beth-at-dogwood.botany.uga.edu]
Sent: Friday, November 17, 2000 8:10 AM
To: microscopy-at-sparc5.microscopy.com


Hi,
We are looking for opinions or reviews on the the Casio QV 3000 digital
camera, with a 340 MB microdrive and macro focusing down to 6 cm.
any help would be greatly appreciated.
thanks,
Beth

**************************************
Beth Richardson
EM Lab Coordinator
Botany Department
University of Georgia
Athens, GA 30602

Phone - (706) 542-1790
FAX - (706) 542-1805
Email - beth-at-dogwood.botany.uga.edu

"Between the two evils,
I always pick the one I never tried before". Mae West (1893-1980)
**************************************






From daemon Mon Nov 20 08:42:01 2000



From: Tamara Howard :      howard-at-cshl.org
Date: Mon, 20 Nov 2000 09:36:00 -0500 (EST)
Subject: Re: Mitochondrial antibodies..

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I've used one to mitochondrial hsp70 from Affinity BioReagents that works
well. Their number is: (800) 278-2424. The catalogue number for the
antibody is MA3-028. In our hands it works in human and mouse
cells....never tried it on birds or inverts.

The other option would be to use one of the MitoTracker dyes from
Molecular probes.

No financial interest in either company, unfortunately!

Tamara Howard
CSHL

On Mon, 20 Nov 2000, Gilles Grondin wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} We are looking for antibodies to mitochondrial proteins , for a
} immunocytochemical study on the effect of parasites in the development of
} youngs birds . Does anyone or company have mitochondrial antibodies ?
}
} Thank you very much .
}
} Gilles Grondin
}
} ggrond01-at-courrier.usherb.ca
}
}
}





From daemon Mon Nov 20 08:45:12 2000



From: Timothy Schneider :      Timothy.Schneider-at-Mail.TJU.EDU
Date: Mon, 20 Nov 2000 09:33:34 -0500
Subject: ALTERNATIVE POST STAIN

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


This is for the person asking about alternative post stains. Instead of
using lead after uranyl acetate I have been very happy with bismuth (I have
been looking at various biological samples embedded in Spurrs).

STOCK SOLUTION: In 10ml of DH2O add 0.2g of NaOh, 400mg of sodium tartrate,
and 200mg of bismuth subnitrate. Shake until dissolved and store up to 2
weeks in the refrigerator.

WORKING SOLUTION: add 20 micro liters of stock to 1ml of DH2O and float the
grids on 50 micro liter drops of this for 5 minutes and rinse with H2O.

Good Luck, Tim

Timothy G. Schneider
Director of Electron Microscopy
Department of Pathology
Room 229 Jefferson Hall
Thomas Jefferson University
1020 Locust St.
Philadelphia Pa. 19107
215-503-4798 work
610-613-8170 cellular
timothy.schneider-at-mail.tju.edu




From daemon Mon Nov 20 08:57:33 2000



From: Donald Lovett :      lovett-at-tcnj.edu
Date: Mon, 20 Nov 2000 09:51:29 -0500 (EST)
Subject: Film dryers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Years ago I worked in a lab that had a small forced (and filtered) film
dryer (with heater). It was just large enough to hold two racks of TEM
negatives. I cannot find one in any of the catalogues. I can only find
film dryers for 35 mm roll film. Could anyone please recommend a source
of a similar dryer? (Vendors, please feel free to respond directly to
me).

Thanks,

Don

______________________________________________________________________
Donald L. Lovett e-mail: lovett-at-tcnj.edu
Assoc. Professor, Dept. of Biology voice: (609) 771-2876
P.O. Box 7718 fax: (609) 637-5118
The College of New Jersey
Ewing, NJ 08628-0718






From daemon Mon Nov 20 09:36:32 2000



From: Kelloes, Cathy L :      KELLOECL-at-bp.com
Date: Mon, 20 Nov 2000 09:28:17 -0600
Subject: SEM-Image system

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

I am trying to find out information on a good imaging system(photographic
only) that could be attached to my SEM, which is an older ABT-55. I do not
have the funds to buy a newer microscope, so I would appreciate any input
you all have. The cost of Polaroid film is extremely expensive, so I am
trying to find a system that I could send digital images through the e-mail
and/or print them instead of using this costly film. Thank you in advance.

Cathy Kelloes
Microscopy Technician
bp Fabrics & Fibers Unit
260 The Bluffs
Austell, GA. 30168
(770) 941-1711, Ext. 3255





From daemon Mon Nov 20 09:38:18 2000



From: Leona Cohen-Gould :      lcgould-at-mail.med.cornell.edu
Date: Mon, 20 Nov 2000 10:35:34 -0500
Subject: Re: help for a HS student

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Listers,

My friend's son is writing a report on oxytocin and called me to ask if I
had or could find any images of it. Its the typical "call X, s/he is a
scientist" type referal.
Does anyone out there have any images, or does anyone know of a good web
site I can send him to? I assume that polarization optics images of the
crystalized molecule would be pretty, but anything would be more than he
currently has.

Please respond to me off-list, and I will forward the info to him.

TIA,
Lee

Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175





From daemon Mon Nov 20 10:50:31 2000



From: Peggy Sherwood :      sherwood-at-helix.mgh.harvard.edu
Date: Mon, 20 Nov 2000 11:45:34 -0500
Subject: NESM Annual December Symposium-Dec. 1st

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


To all:

The 34th Annual NESM (New England Society for Microscopy) Symposium
will be held at the Newton Marriott, Newton, Massachusetts on Friday,
December 1st from Noon to 9:30 pm.

The meeting will consist of 2 sessions.
SESSION I (1-2:30 pm) will focus on Raman and FT-IR Spectroscopy.
Speakers will include Barbara Foster-"Bridging the
Microscopy-Spectroscopy Chasm", John Reffner-"Resolving Molecular
Chemistry with FT-IR Microspectroscopy" and Patrick Treado-"Chemical
Imaging: A Powerful Tool for Molecular Microscopy".

A Poster Session/Contest will then run concurrent with the coffee break (2:30-
3:15 pm) (details below).

SESSION II (3:15-4:45 pm) will focus on Genechip Technology. Speakers for that
session will include: Eiman H. Al-Mutari-"The Human Genome Study: cDNA Micro-
arrays and Signal Amplification", Joseph Paulauskis-"Microarray Technologies:
Promise and Problems", and Steven Lott-"Microarray Technology: Changing the
Face of Functional Genomics".

The annual business meeting/election of new officers will convene at 5:00 pm,
followed by a Cocktail Hour (6-7 pm) and dinner (7-8 pm). The after
dinner speaker will be Paul Hlava, MAS Tour Speaker, from Sandia
National Labs whose
talk will be: "Causes of Color in Minerals and Gemstones".

The DEADLINE for advance registration (and dinner reservations) is Friday,
November 24th. Payment must accompany advance registration form if you wish to
have dinner. The choices for dinner are: herbed crusted chicken, swordfish,
and vegetarian (please choose one). Please contact Mary McCann,
Treasurer, at (617) 484-7865 or by email: mccanns-at-tiac.net for
further information. Send registration forms WITH payment to NESM,
c/o Mary McCann, 161 Claflin Street, Belmont, MA 02478.
(Registration will be held at the meeting from 12Noon-1pm, but will
NOT include dinner).

Poster abstracts should be sent to: Christopher Santeufemio,
Biological Director at: 57 Bancroft Street, Pepperell, MA 01463 by
November 24th. Any questions, contact Chris at (978) 433-8031 or by
email: csanteufemio-at-epion.com.
Prizes will be awarded for Best Poster, Second and Third Place.

We hope to see you there at this most interesting meeting!

Peggy Sherwood
Corresponding Secretary, NESM

--
Peggy Sherwood
Lab Associate, Photopathology
Wellman Laboratories of Photomedicine (W224)
Massachusetts General Hospital
50 Blossom Street
Boston, MA 02114
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-3192 (fax)
sherwood-at-helix.mgh.harvard.edu



From daemon Mon Nov 20 13:02:31 2000



From: Paula Sicurello :      patpxs-at-gwumc.edu
Date: Mon, 20 Nov 2000 14:01:54 -0500
Subject: I'm hooked on you...

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi there my esteemed listers,

I've been asked to do some TEM on hookworms. I've never even seen a hookworm before, let alone do EM on one. I've heard that they're tough customers when it comes to fixation.

Does anybody out there have a protocol that is fairly simple but results in good utlrastructural preservation?

Drop me a line if you do.

I'm sinking without your help.


Paula :-)
patpxs-at-gwumc.edu






From daemon Mon Nov 20 13:23:21 2000



From: Walck, Scott D. :      walck-at-ppg.com
Date: Mon, 20 Nov 2000 14:20:26 -0500
Subject: RE: Film dryers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


There is a company called California Stainless that made film dryers that
some of the EM supply houses sold. I bought one from them a number of years
ago. Sorry that I don't have the contact info, but I'm sure that this is
just what you want.


-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)



-----Original Message-----
From: Donald Lovett [mailto:lovett-at-tcnj.edu]
Sent: Monday, November 20, 2000 9:51 AM
To: Microscopy.lst
Subject: Film dryers


---------------------------------------------------------------
---------
The Microscopy ListServer -- Sponsor: The Microscopy Society
of America
To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
On-Line Help
http://www.msa.microscopy.com/MicroscopyListserv er/FAQ.html



---------------------------------------------------------------
--------.



Years ago I worked in a lab that had a small forced (and filtered) film
dryer (with heater). It was just large enough to hold two racks of TEM
negatives. I cannot find one in any of the catalogues. I can
only find
film dryers for 35 mm roll film. Could anyone please
recommend a source
of a similar dryer? (Vendors, please feel free to respond directly to
me).

Thanks,

Don

______________________________________________________________________
Donald L. Lovett e-mail: lovett-at-tcnj.edu
Assoc. Professor, Dept. of Biology voice: (609) 771-2876
P.O. Box 7718 fax: (609) 637-5118
The College of New Jersey
Ewing, NJ 08628-0718







From daemon Mon Nov 20 14:42:56 2000



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Mon, 20 Nov 2000 20:37:17 +0000 (GMT Standard Time)
Subject: Re: ALTERNATIVE POST STAIN

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Just curious - why do you use bismuth?

Dave


On Mon, 20 Nov 2000 09:33:34 -0500 Timothy Schneider
{Timothy.Schneider-at-Mail.TJU.EDU} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} This is for the person asking about alternative post stains. Instead of
} using lead after uranyl acetate I have been very happy with bismuth (I have
} been looking at various biological samples embedded in Spurrs).
}
} STOCK SOLUTION: In 10ml of DH2O add 0.2g of NaOh, 400mg of sodium tartrate,
} and 200mg of bismuth subnitrate. Shake until dissolved and store up to 2
} weeks in the refrigerator.
}
} WORKING SOLUTION: add 20 micro liters of stock to 1ml of DH2O and float the
} grids on 50 micro liter drops of this for 5 minutes and rinse with H2O.
}
} Good Luck, Tim
}
} Timothy G. Schneider
} Director of Electron Microscopy
} Department of Pathology
} Room 229 Jefferson Hall
} Thomas Jefferson University
} 1020 Locust St.
} Philadelphia Pa. 19107
} 215-503-4798 work
} 610-613-8170 cellular
} timothy.schneider-at-mail.tju.edu
}
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"




From daemon Mon Nov 20 15:23:41 2000



From: Gary Gaugler :      gary-at-gaugler.com
Date: Mon, 20 Nov 2000 13:14:59 -0800
Subject: Re: SEM-Image system

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


See if an active or passive digital control & capture
unit would work on your SEM. Soft Imaging makes
ADDA which does active and passive very nicely.
There are others which do active and some which
only do passive. I prefer active. But if your system
can't be adapted to active, perhaps it will support
passive. If not, maybe active.

Several of the suppliers are on this list. Perhaps they
will contact you. I use ADDA and like it very much.
It is actually more than just SEM image capture.
I use it to capture chamber TV camera video and
SEM TV scan images as well as up to 4096x4096 pixel
active scan SEM image files (TIFF).

gary


At 07:28 AM 11/20/00, you wrote:

} Hi,
}
} I am trying to find out information on a good imaging system(photographic
} only) that could be attached to my SEM, which is an older ABT-55. I do not
} have the funds to buy a newer microscope, so I would appreciate any input
} you all have. The cost of Polaroid film is extremely expensive, so I am
} trying to find a system that I could send digital images through the e-mail
} and/or print them instead of using this costly film. Thank you in advance.
}
} Cathy Kelloes
} Microscopy Technician
} bp Fabrics & Fibers Unit
} 260 The Bluffs
} Austell, GA. 30168
} (770) 941-1711, Ext. 3255




From daemon Mon Nov 20 17:15:27 2000



From: Al Coritz :      al-at-boeckeler.com
Date: Mon, 20 Nov 2000 16:09:59 -0700
Subject: Re: Automatic sample processors for EM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Everyone:

The RMC "EMP 5160" EM tissue processor is available through Boeckeler
Instruments as is the entire RMC Instrument catalog. For more information
visit our website: www.rmcproducts.com

Al Coritz
Sales & Service Manager
RMC-Boeckeler Instruments
4650 S. Butterfield Dr.
Tucson, AZ 85714
Voice: 520-745-0001
Cell: 520-465-3598
Fax: 520-745-0004
Email:Al-at-Boeckeler.com
Website:RMCProducts.com


-----Original Message-----
} From: Roger Moretz [mailto:rcmoretz-at-excite.com]
Sent: Monday, November 20, 2000 6:00 AM
To: Marek Malecki; Microscopy-at-sparc5.microscopy.com


Mareck:

I have responded to these queries before, so here goes again. The unit I
have used for years is the Lynx Tissue Processor (currently available from
EM Sciences). The original unit I purchased (from the manufacturer) was
marvelous. A second unit (purchased when Leica was marketing it) took over
5 years to get it into reliable working condition. My guess is that EMS
will provide a reliable product. The only other unit I know of is sold by
RMC (recently cycled through Ventana and now Boekeler -- Tucson, AZ) that is
an updated version of the old LKB design. I know of very happy users for
this product, but I honestly do not know the current status under the new
owners. I have attempted to get a demo of the RMC device, but RMC never
followed through, and the last incarnations have occurred so quickly that I
still can't provide a personal comparison.

Roger Moretz, Ph.D.
Dept of Toxicology
Boehringer Ingelheim Pharmaceuticals, Inc.
On Fri, 17 Nov 2000 00:19:10 -0800, Marek Malecki wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I would appreciate comments and suggestions on automatic sample
processors
} for EM. Vendors welcome.
} Marek Malecki, M.D., Ph.D.
} Director and Principal Investigator
}
} Molecular Imaging Laboratories
} University of California, San Diego
}
} address: 1500 Suite Bonner Hall, 9500 Gilman Drive, La Jolla,
CA92093-0368
} phone - office: 8588223373
} phone - cell: 8583443347
} fax: 8588223715
} e.mail: mmm-at-ucsd.edu
} pager: 8586161420
} e.mail: mmm-at-ucsd.edu
} e.pager: 1620024619-at-alphapage.airtouch.com
} www site: http://mil.ucsd.edu/
} ftp site: mil1.ucsd.edu
}
}
}





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From daemon Mon Nov 20 18:19:28 2000



From: Awbrey, Donald :      DonaldAwbrey-at-texashealth.org
Date: Mon, 20 Nov 2000 18:13:25 -0600
Subject: Re:Thanks for info on TEM stains

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Dear TEM netters,

Thanks again for all of the responses. They were very
informative.

We primarily perform TEM on renal biopsies particularly
the Bowman's capsule and glomerulus. The main ultra-
structure we concentrate on is the basement membranes of
the glomerulus. Less frequently we perform TEM on tumor
tissues. This is done in a clinical setting.

Any other information out there in the "Netter Land" will
be appreciated.

Thanks Again,


Donald G. Awbrey, HT(ASCP) QIHC
Electron Microscopy / Image Analysis
DonaldAwbrey-at-TexasHealth.org
donaldawbrey-at-hotmail.com
(817)-878-5647





From daemon Mon Nov 20 19:02:37 2000



From: Thor Bostrom :      t.bostrom-at-qut.edu.au
Date: Tue, 21 Nov 2000 10:56:48 +1100
Subject: Re: SVMicro Digital Microscope Camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 16:37 16/11/00 -0500, you wrote:
} Dear Listers,
} Does anyone have an experience that they would like to share involving the
} SVMicro digital camera by Sound Vision? I'm hoping to learn anything at
} all, from delivery, support, service, image quality, product reliability,
} etc. Thanks in advance.
} Bill Roberts


We have an SVMicro attached to a Nikon Labophot-Pol petrographic
microscope, with a Nikon 1x relay lens in the adaptor. We also use it with
a Zeiss Jenaval. Alternatively you can just screw a lens on it and point it
at something (with a tripod, preferably). The camera runs off a SCSI card
in a PC.

Our experience is that the Twain interface software is a little tricky to
use, and it's difficult for new users to get good images until you have
done some tweaking. You usually need to turn the illumination down a bit,
or use a neutral density filter. The colour balance needs to be corrected
-- I use a neutral density filter as the specimen to set the neutral gray
balance -- but a pale blue filter may also work. The images tend to be very
contrasty unless you set the gamma level (the middle triangle on the
intensity histogram) a long way to the left. Adjusting the levels tends to
be very touchy, as compared to say Photoshop. However once you get the
exposure time right you can get very nice RGB images, at a true pixel
resolution of about 1000x800.


Thor Bostrom
Analytical EM Facility
Faculty of Science
Queensland University of Technology (QUT)
PO Box 2434, Brisbane, QLD 4001
AUSTRALIA




From daemon Tue Nov 21 02:29:00 2000



From: Keith Ryan :      kpr-at-pml.ac.uk
Date: Tue, 21 Nov 2000 08:15:29 +0000
Subject: Re: I'm hooked on you...

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Paula

I may have a copy of a paper somewhere which advocated hot fixatives
for nematodes because t worked better than room temp/cold fixatives.
Purely on penetration? Getting through the cuticle? Come back if
you're still desperate - I think it is in my cellar somewhere at
home!

Keith Ryan
PLymouth, UK



From daemon Tue Nov 21 04:25:02 2000



From: Thor Bostrom :      t.bostrom-at-qut.edu.au
Date: Tue, 21 Nov 2000 16:42:07 +1100
Subject: Crystallites in glass ceramic

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



A colleague here is trying to determine the presence and size of
microcrystallites in a glass ceramic. It is an alkali-lime borosilicate
glass, which appears to be amorphous by XRD. However it has a translucent
appearance which suggests that it may contain microcrystallites or some
other defects, though these are not evident by optical microscopy.

The suggestions so far are: (a) light etching of a polished surface,
followed by examination in a FESEM; or (b) preparation of an ion beam
thinned sample for TEM.

We would welcome any other suggestions. For example, would EBSD work? And
if we tried (a), is there a suitable etchant (dilute HF?) for this material?


With thanks,

Thor Bostrom
Analytical EM Facility
Faculty of Science
Queensland University of Technology (QUT)
PO Box 2434, Brisbane, QLD 4001
AUSTRALIA
Email: t.bostrom-at-qut.edu.au

=-=-=-=-=-=-=-=-=-=-=-=-=-=
Dr Thor Bostrom
Ext. 2351
Analytical EM Facility,
Faculty of Science, QUT
t.bostrom-at-qut.edu.au
=-=-=-=-=-=-=-=-=-=-=-=-=-=




From daemon Tue Nov 21 06:27:28 2000



From: Johan Hazekamp :      Johan.Hazekamp-at-unilever.com
Date: Tue, 21 Nov 2000 11:24:32 +0100
Subject: TEM/SEM: Tracer elements in Lowicryl

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear All,

Does anyone have suggestions how to add a label/element to Lowicryl HM20.
We would like to differentiate/locate the Lowicryl in some porous structures
using EDX.

thanks,

Johan Hazekamp

**************************************************
Johan Hazekamp
Central Analytical Science
Unilever Research Vlaardingen
P.O. Box 114, 3130 AC Vlaardingen
The Netherlands
Tel.: (31) 10-4605530
Fax: (31) 10 4605671
Email: Johan.Hazekamp-at-unilever.com




From daemon Tue Nov 21 08:14:14 2000



From: Ronald Anderson :      anderron-at-US.ibm.com
Date: Tue, 21 Nov 2000 08:59:49 -0500
Subject: Crystallites in glass ceramic

Contents Retrieved from Microscopy Listserver Archives
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TEM examination is best and would allow identification of the crystallites.
Ion milling silicates is potentially a problem. I'd suggest choosing a
method that doesn't involve much, if any, ion milling, such as cleaving,
crushing, or tripod polishing.

Ron Anderson, IBM, Hopewell Jct., New York, USA. anderron-at-us.ibm.com
IBM Analytical Services; http://www.chips.ibm.com/services/asg


Thor Bostrom {t.bostrom-at-qut.edu.au} on 11/21/2000 12:42:07 AM

To: microscopy-at-sparc5.microscopy.com
cc:



A colleague here is trying to determine the presence and size of
microcrystallites in a glass ceramic. It is an alkali-lime borosilicate
glass, which appears to be amorphous by XRD. However it has a translucent
appearance which suggests that it may contain microcrystallites or some
other defects, though these are not evident by optical microscopy.

The suggestions so far are: (a) light etching of a polished surface,
followed by examination in a FESEM; or (b) preparation of an ion beam
thinned sample for TEM.

We would welcome any other suggestions. For example, would EBSD work? And
if we tried (a), is there a suitable etchant (dilute HF?) for this
material?


With thanks,

Thor Bostrom
Analytical EM Facility
Faculty of Science
Queensland University of Technology (QUT)
PO Box 2434, Brisbane, QLD 4001
AUSTRALIA
Email: t.bostrom-at-qut.edu.au

=-=-=-=-=-=-=-=-=-=-=-=-=-=
Dr Thor Bostrom
Ext. 2351
Analytical EM Facility,
Faculty of Science, QUT
t.bostrom-at-qut.edu.au
=-=-=-=-=-=-=-=-=-=-=-=-=-=








From daemon Tue Nov 21 08:22:43 2000



From: Warren E Straszheim :      wesaia-at-iastate.edu
Date: Tue, 21 Nov 2000 08:14:25 -0600
Subject: Re: TEM/SEM: Tracer elements in Lowicryl

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In the early 1980s, we followed someone's suggestion to dissolve Iodoform
in epoxy resin to raise its average atomic number to provide contrast with
coal particles. It also provided elemental contrast.

It worked with epoxy; it setup just fine. But I am not sure how it would
work Lowicryl. It does list a number of precautions listed on the MSDS.

At 11:24 AM 11/21/2000 +0100, you wrote:

} Dear All,
}
} Does anyone have suggestions how to add a label/element to Lowicryl HM20.
} We would like to differentiate/locate the Lowicryl in some porous structures
} using EDX.
}
} thanks,
}
} Johan Hazekamp
}
} **************************************************
} Johan Hazekamp
} Central Analytical Science
} Unilever Research Vlaardingen
} P.O. Box 114, 3130 AC Vlaardingen
} The Netherlands
} Tel.: (31) 10-4605530
} Fax: (31) 10 4605671
} Email: Johan.Hazekamp-at-unilever.com
}




From daemon Tue Nov 21 08:24:25 2000



From: dzhao :      dzhao-at-biol.sc.edu
Date: Tue, 21 Nov 2000 09:34:31 -0500
Subject: Re: Crystallites in glass ceramic

Contents Retrieved from Microscopy Listserver Archives
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Johan
Polysciences http://www.polysciences.com/ list barium
methacrylate, lead methacrylate and lead acrylate, all of which can
be used to impart x-ray / electron opacity to acrylic polymers. I
don't know how you would formulate Lowicryl to take advantage of
this, but it looks like a possible starting point. I would be very
interested to know of specific formulations for using these
compounds in EM grade embedding, because I have a related
application.

Best wishes
Chris

Date sent: Tue, 21 Nov 2000 11:24:32 +0100
} From: "Johan Hazekamp" {Johan.Hazekamp-at-unilever.com}


Microcrystallites can be identified using back scattered electron image on
a polished surface of the sample. If the microcrystallites are big enough,
e.g., a few microns or larger, its chemical composition can be determined
on a polished surface using electron microprobe. In some cases, the shapes
and chemical composition of the phase will be enough to determine whether
the phase is crystalline.

At 04:42 PM 11/21/00 +1100, Thor Bostrom wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From daemon Tue Nov 21 09:43:42 2000



From: Kelloes, Cathy L :      KELLOECL-at-bp.com
Date: Tue, 21 Nov 2000 09:31:34 -0600
Subject: SEM Image System-Thank You

Contents Retrieved from Microscopy Listserver Archives
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To everyone who sent me information on SEM image systems, I want to tell you
how much I appreciate it. I have received so many good suggestions and
references that I now will be able to make a more knowledgeable decision.
Once again, thank you so much.

Cathy Kelloes
Microscopy Technician
bp Fabrics & Fibers Unit
260 The Bluffs
Austell, GA. 30168
(770) 941-1711, Ext. 3255





From daemon Tue Nov 21 09:51:48 2000



From: Stephen Wood :      stephenwood-at-meridiansci.com
Date: Tue, 21 Nov 2000 10:50:18 -0500
Subject: Zeiss EM109 Specimen Holder

Contents Retrieved from Microscopy Listserver Archives
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Hello All

I was wondering if anyone can help me find a tiltable and rotatable specimen
cartridge for a Zeiss EM 109 TEM.
Vendors quite welcome!

Thanks very much for your help

Kindest Regards


Stephen Wood
Meridian Scientific Services Inc.
Ottawa Canada
Tel: 613-836-6749
Fax: 613-836-5880
e-Fax:413-460-3007
e-mail: stephenwood-at-meridiansci.com
web site: http://meridiansci.com/







From daemon Tue Nov 21 12:08:29 2000



From: Tom Phillips :      PhillipsT-at-missouri.edu
Date: Tue, 21 Nov 2000 11:59:23 -0600
Subject: effect of pH on osmication??

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Any one have a reference on the effect of pH on the osmication
reaction? My attempts at a literature search on this topic have not
been much success. Happy Thanksgiving.

--
Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)



From daemon Tue Nov 21 12:11:46 2000



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Tue, 21 Nov 2000 10:07:10 -0800
Subject: Re: SEM-Image system

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Cathy,
You may find that Quartz PCI, sold by Hitachi Instruments, is most suitable
for passively capturing images from an older SEM. You get an image that is
very close to the Poaroid image, stored on the computer in your choice of 18
different formats. Visit them at: www.quartzimaging.com.
At 09:28 AM 11/20/00 -0600, you wrote:
}
} Hi,
}
} I am trying to find out information on a good imaging system(photographic
} only) that could be attached to my SEM, which is an older ABT-55. I do not
} have the funds to buy a newer microscope, so I would appreciate any input
} you all have. The cost of Polaroid film is extremely expensive, so I am
} trying to find a system that I could send digital images through the e-mail
} and/or print them instead of using this costly film. Thank you in advance.
}
} Cathy Kelloes
} Microscopy Technician
} bp Fabrics & Fibers Unit
} 260 The Bluffs
} Austell, GA. 30168
} (770) 941-1711, Ext. 3255

Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca




From daemon Tue Nov 21 12:24:39 2000



From: Adriana Pinheiro Martinelli Rodriguez :      adriana-at-cena.usp.br
Date: Tue, 21 Nov 2000 16:19:45 -300
Subject: Hello from Brazil...

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Cathy:

I saw your messages in the microscopy list and was so happy to "hear" from you. I don't know if you'll remember me, I used to be a student at the Hort Department with Dr. Hazel Wetzstein and took both EM courses at the EM center in Athens.

I'm back in Brazil since 1994 and I've been back to Athens a few times after that. I visited most of the people I've known and missed seeing you there. I knew you're in Atlanta from Dr. Farmer.

How are things going with you? Do you still have horses? I see you are still working with EM, which is good!

Well, whenever you have a chance, let me know how things are going. I'm going to the US in December January and will spend some time in Athens and also will visit some friends in Atlanta. Maybe I'll have a chance to meet you again.

Take care, regards,

Adriana

Adriana P. M. Rodriguez
Laboratório de Biotecnologia Vegetal
CENA, Universidade de São Paulo
adriana-at-cena.usp.br





From daemon Tue Nov 21 13:25:06 2000



From: OCONNELL-at-ltu.edu
Date: Tue, 21 Nov 2000 14:19:37 -0500 (EST)
Subject: Sem: Need manuals for an ISI-SS40

Contents Retrieved from Microscopy Listserver Archives
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Being new to this system, I don't know how this is going to work.
However, I purchased an ISI-SS40 a few year ago, but I did't get
any manuals with it. It appears to work ok, but there are a lot
of questions. If anynoe



Knows where I can get manuals for this
particular instrument I would greatly appreciate hearing from you.
You may e-mail me at oconnell-at-ltu.edu or call 734-668-3309and I
will get back with you.

Thank You

Dick O'Connell



From daemon Tue Nov 21 13:47:31 2000



From: Russell E. Cook :      recook-at-anl.gov
Date: Tue, 21 Nov 2000 13:45:09 -0600
Subject: Re: Crystallites in glass ceramic

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I don't see how examination in a FESEM will be helpful.
I doubt that EBSD will work because of the volume required to produce a
pattern.
I think that TEM is the only way to get the information.
..Russ Cook
} -----------------------------------------------------------------------
}
} A colleague here is trying to determine the presence and size of
} microcrystallites in a glass ceramic. It is an alkali-lime borosilicate
} glass, which appears to be amorphous by XRD. However it has a translucent
} appearance which suggests that it may contain microcrystallites or some
} other defects, though these are not evident by optical microscopy.
}
} The suggestions so far are: (a) light etching of a polished surface,
} followed by examination in a FESEM; or (b) preparation of an ion beam
} thinned sample for TEM.
}
} We would welcome any other suggestions. For example, would EBSD work? And
} if we tried (a), is there a suitable etchant (dilute HF?) for this material?
}
}
} With thanks,
}
} Thor Bostrom
} Analytical EM Facility
} Faculty of Science
} Queensland University of Technology (QUT)
} PO Box 2434, Brisbane, QLD 4001
} AUSTRALIA
} Email: t.bostrom-at-qut.edu.au
}
} =-=-=-=-=-=-=-=-=-=-=-=-=-=
} Dr Thor Bostrom
} Ext. 2351
} Analytical EM Facility,
} Faculty of Science, QUT
} t.bostrom-at-qut.edu.au
} -----------------------------------------------------------------------


----------------------------------------------------------------------
Russell E. Cook, Ph.D.
Electron Microscopy Center
Argonne National Laboratory
Materials Science Division, Building 212
9700 South Cass Avenue
Argonne, IL 60439-4838
(630)252-7194
FAX: (630)252-4289
recook-at-anl.gov





From daemon Tue Nov 21 14:06:33 2000



From: treese :      treese-at-marinebio.mbl.edu
Date: Tue, 21 Nov 2000 15:08:22 -0800
Subject: Gatan rotation/tilt holder for Philips CM120 needed

Contents Retrieved from Microscopy Listserver Archives
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We need another specimen holder for a Philips /FEI CM 120. Gatan
made a perfect holder with ±60 tilt & 360° of rotation. I am not
looking for a cryoholder. Hope someone has one of these Gatan gems
on their shelf that they would like to sell to us. thanks...Tom
Reese, NIH



From daemon Tue Nov 21 14:11:02 2000



From: A.G.Cullis :      A.G.Cullis-at-sheffield.ac.uk
Date: Tue, 21 Nov 2000 20:08:17 -0000
Subject: MSMXII Conference: Final Call for Papers

Contents Retrieved from Microscopy Listserver Archives
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Royal Microscopical Society

12th International Conference on

MICROSCOPY OF SEMICONDUCTING MATERIALS

25-29 March 2001, University of Oxford, UK

************************************************
Final Call for Papers
************************************************

This international conference will focus on the latest developments
in the study of the structural and electrical properties of
semiconductors by the application of transmission and scanning
electron microscopy, scanning probe microscopy and X-ray
techniques.

The state-of-the-art in all important subject areas will be
addressed, including the characterisation of bulk and thin film
as-grown materials, the study of lattice defect and impurity
behaviour and the investigation of advanced semiconductor
processing procedures.

Special conference sessions will concentrate on recent
developments in high-resolution imaging and analytical electron
microscopy, advances in SEM and SPM applications, the
characteristics of epitaxial layers (including III-V nitrides),
quantum wells, wires and dots, the effects of device processing
treatments (including, especially, advanced silicon technology) and
metal-semiconductor contacts and silicides. Prominent invited
speakers will introduce each topic area.

The Proceedings of the conference will be published and the final
call for papers has now been issued. Abstracts (deadline 1
DECEMBER 2000) should be submitted by E-mail to:
jenny-at-rms.org.uk

Full conference information (with the invited speaker listing, etc)
can be found at the conference Web site
http://www.rms.org.uk/currentevents2.htm#MSMXII

The conference Chairmen are Prof Tony Cullis
(a.g.cullis-at-sheffield.ac.uk) and Dr John Hutchison
(john.hutchison-at-materials.ox.ac.uk) who may be contacted with
any general enquiries.

************************************************



From daemon Tue Nov 21 14:16:09 2000



From: A.G.Cullis :      A.G.Cullis-at-sheffield.ac.uk
Date: Tue, 21 Nov 2000 20:13:51 -0000
Subject: MSMXII Conference: Final Call for Papers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Royal Microscopical Society

12th International Conference on

MICROSCOPY OF SEMICONDUCTING MATERIALS

25-29 March 2001, University of Oxford, UK

************************************************
Final Call for Papers
************************************************

This international conference will focus on the latest developments
in the study of the structural and electrical properties of
semiconductors by the application of transmission and scanning
electron microscopy, scanning probe microscopy and X-ray
techniques.

The state-of-the-art in all important subject areas will be
addressed, including the characterisation of bulk and thin film
as-grown materials, the study of lattice defect and impurity
behaviour and the investigation of advanced semiconductor
processing procedures.

Special conference sessions will concentrate on recent
developments in high-resolution imaging and analytical electron
microscopy, advances in SEM and SPM applications, the
characteristics of epitaxial layers (including III-V nitrides),
quantum wells, wires and dots, the effects of device processing
treatments (including, especially, advanced silicon technology) and
metal-semiconductor contacts and silicides. Prominent invited
speakers will introduce each topic area.

The Proceedings of the conference will be published and the final
call for papers has now been issued. Abstracts (deadline 1
DECEMBER 2000) should be submitted by E-mail to:
jenny-at-rms.org.uk

Full conference information (with the invited speaker listing, etc)
can be found at the conference Web site
http://www.rms.org.uk/currentevents2.htm#MSMXII

The conference Chairmen are Prof Tony Cullis
(a.g.cullis-at-sheffield.ac.uk) and Dr John Hutchison
(john.hutchison-at-materials.ox.ac.uk) who may be contacted with
any general enquiries.

************************************************



From daemon Tue Nov 21 16:22:29 2000



From: Michael Coviello :      coviello-at-mae.uta.edu
Date: Tue, 21 Nov 2000 16:26:10 -0600
Subject: TEM-looking for a annealing holder that fits a JEOL 100CX/200CX/1200EX

Contents Retrieved from Microscopy Listserver Archives
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Hi Y'all:
We're looking to buy or trade for an annealing holder for a
100CX/200CX/1200 EX TEM's (fits all of these and probably others). We
hope someone has one that they haven't used in years and would like to
get some money out of it Please let us know.
Regards,
Mike Coviello
Lab Manager
UT Arlington
817 272-5496




From daemon Tue Nov 21 17:59:48 2000



From: Awbrey, Donald :      DonaldAwbrey-at-texashealth.org
Date: Tue, 21 Nov 2000 17:53:38 -0600
Subject: TEM: Carboy's

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Dear TEM Netters:

Does anyone have information about a supplier of amber
colored carboy containers. We need these carboys to be
about 1-2 gallon in size. They need to made of a plastic
polymer that is resistant to hazardous chemicals. They are
to be used to store stock film processing fluids. Any info
would be greatly appreciated.

Thanks in advance.


Donald G. Awbrey, HT(ASCP) QIHC
Electron Microscopy / Image Analysis
DonaldAwbrey-at-TexasHealth.org
donaldawbrey-at-hotmail.com
(817)-878-5647





From daemon Wed Nov 22 00:54:23 2000



From: COURYHOUSE-at-aol.com
Date: Wed, 22 Nov 2000 01:43:20 EST
Subject: Has anyone out there used an AO comparison microscope before?

Contents Retrieved from Microscopy Listserver Archives
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Ahoy fellow enthusiasts!
Has anyone out there used a comparison microscope before?
I got a bridge unit and dropped it on top of 2 AO 10 scopes, there seems to
me a knob on the front that does nothing. I get one scope in once side of
the field and the image from the other scope on the other side.....

I suspect the knob makes them overlap (like we have seen on the police
shows but it does not seem to move the image.
when they compare bullets...)

any input?

thanks Ed Sharpe archivist for SMECC






From daemon Wed Nov 22 07:05:20 2000



From: Roger Moretz :      rcmoretz-at-excite.com
Date: Wed, 22 Nov 2000 04:53:07 -0800 (PST)
Subject: Re: effect of pH on osmication??

Contents Retrieved from Microscopy Listserver Archives
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Tom:

The original volume of Hayat's Principles & Techniques of Electron
Microscopy (vol. 1), now long out of print, discusses this in chapter 1. He
references a paper by Trump and Ericsson (Lab Invest 14:1245, 1965) as a
primary reference. In fact, if you were to review Ben Trump's literature,
you would find a wealth of information on the role of pH, osmolarity,
fixative concentrations and types, etc. Most of this has been summarized by
Hayat in various volumes and in the Glauert series. A quick on-line search
at PubMed will give you enough references to bury you! However, using
interlibrary loan you should be able to at least get the Glauert series,
altho' I think that precious few libraries (including most EM labs) have the
Hayat books. Although I can't lay my hands on a lot of books right now, I
would also have thought that any basic book on TEM would cover your
question, so if you have access to any of the recent books, check out the
chapter on fixatives and fixation.

Roger Moretz, Ph.D.
Dept. of Toxicology
Boehringer Ingelheim Pharmaceuticals

On Tue, 21 Nov 2000 11:59:23 -0600, Tom Phillips wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Any one have a reference on the effect of pH on the osmication
} reaction? My attempts at a literature search on this topic have not
} been much success. Happy Thanksgiving.
}
} --
} Thomas E. Phillips, Ph.D.
} Associate Professor of Biological Sciences
} Director, Molecular Cytology Core Facility
}
} 3 Tucker Hall
} Division of Biological Sciences
} University of Missouri
} Columbia, MO 65211-7400
} (573)-882-4712 (voice)
} (573)-882-0123 (fax)
}





_______________________________________________________
Tired of slow Internet? Get -at-Home Broadband Internet
http://www.home.com/xinbox/signup.html




From daemon Wed Nov 22 08:20:32 2000



From: Bemporad, Edoardo :      e.bemporad-at-materials10.dimi.uniroma3.it
Date: Wed, 22 Nov 2000 08:09:34 -0600
Subject: TEM glueing samples; selective solvants

Contents Retrieved from Microscopy Listserver Archives
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Hi everybody,
hope someone can help me:
I need to stick each other two pieces of coated metallic samples to make a
XTEM observation; then I need to stick them (already together bonded!) on
the lapping tool in order to thin them. I have tried to use superglue
(cyanoacrylic glue) for the first operation and thermoplastic glue for the
second. Unfortunately I haven't found nothing to dissolve the thermoplastic
but the superglue.


Does anyone can suggest me two different glue that can be suitable and can
be dissolved selectively?


Thank you in advance, Edoardo.



Dr. Eng. Edoardo Bemporad, Ph. D.
Assistant Professor of Materials Science
University of Rome "Roma Tre" (Italy)
Dipartimento di Ingegneria Meccanica e Industriale
(Department of Mechanical and Industrial Engineering)
Via Vasca Navale 79 - 00146 Rome, Italy
Tel: +39 06 5517.3293
Fax: +39 06 5517.3256
LIME Lab Tel: +39 06 5517.3200
LIME Web Site: http//materials.dimi.uniroma3.it/lime
E-Mail:bemporad-at-uniroma3.it







From daemon Wed Nov 22 10:10:45 2000



From: john david whitaker :      jwhitake-at-u.washington.edu
Date: Wed, 22 Nov 2000 08:05:59 -0800 (PST)
Subject: Re: TEM glueing samples; selective solvants

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I do XTEM of metal films, and have found Gatan's G-1 epoxy
to be effective in creating the "sandwich". I use crystal
bond low-melting wax to bond the sandwich to the polishing
stub for thinning. The former is resistant to most solvents;
the latter dissolves readily in acetone.

I also hear that M-bond 610 adhesive is good for gluing the
halves of the sandwich together, though I have not tried
this brand.

Good luck!

John





From daemon Wed Nov 22 10:28:19 2000



From: David Henriks :      Henriks-at-CompuServe.COM
Date: Wed, 22 Nov 2000 11:25:34 -0500
Subject: TEM glueing samples; selective solvants

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Edoardo:

The material you should use to stick the pieces together is Epotek 353ND
which is produced by Epoxy Technology. You can find information on the
product on their website at www.epotek.com/optical.html. Their distributor
in Italy is:

Anna Giallo
Kontek Comatel S.P.A.
Via Rio Vallone #5
20050 Mezzago. Milan
Italy

Tel: 39 039 6883300
Fax: 39 039 6883210
Email: kontek-at-cemb.it
http://www.kontek-comatel.com

The acetone soluble wax that you can use it our QuickStick 135. This comes
in a package with 20 small sticks and is ideal for this application. I
hope this helps!

Best regards-

David
Writing at 9:09:28 AM on 11/22/2000

***************************************************************************
************************

David Henriks
Vice President TEL: 800-728-2233
(toll free in the USA)
South Bay Technology, Inc. +1-949-492-2600
1120 Via Callejon FAX: +1-949-492-1499
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

***************************************************************************
************************

} } } } } Please visit us at http://www.southbaytech.com { { { { {

Manufacturers of precision sample preparation equipment and supplies for
metallography, crystallography and electron microscopy.

Message text written by "Bemporad, Edoardo"
}
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Hi everybody,
hope someone can help me:
I need to stick each other two pieces of coated metallic samples to make a
XTEM observation; then I need to stick them (already together bonded!) on
the lapping tool in order to thin them. I have tried to use superglue
(cyanoacrylic glue) for the first operation and thermoplastic glue for the
second. Unfortunately I haven't found nothing to dissolve the thermoplastic
but the superglue.


Does anyone can suggest me two different glue that can be suitable and can
be dissolved selectively?


Thank you in advance, Edoardo.



Dr. Eng. Edoardo Bemporad, Ph. D.
Assistant Professor of Materials Science
University of Rome "Roma Tre" (Italy)
Dipartimento di Ingegneria Meccanica e Industriale
(Department of Mechanical and Industrial Engineering)
Via Vasca Navale 79 - 00146 Rome, Italy
Tel: +39 06 5517.3293
Fax: +39 06 5517.3256
LIME Lab Tel: +39 06 5517.3200
LIME Web Site: http//materials.dimi.uniroma3.it/lime
E-Mail:bemporad-at-uniroma3.it



{



From daemon Wed Nov 22 10:44:01 2000



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Wed, 22 Nov 2000 08:40:51 -0800
Subject: Re: Crystallites in glass ceramic

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Thor,
EBSP might work, if the micro-crystallites are larger than the e-beam size.
The specimen preparation is the same as for rocks for EBSP: polish down to
0.01 micron or Syton (colloidal silica) level. Best to ask someone who has
done it. Try the web sites of HKL (www.channel.dk) or TSL.
At 04:42 PM 11/21/00 +1100, you wrote:
}
} A colleague here is trying to determine the presence and size of
} microcrystallites in a glass ceramic. It is an alkali-lime borosilicate
} glass, which appears to be amorphous by XRD. However it has a translucent
} appearance which suggests that it may contain microcrystallites or some
} other defects, though these are not evident by optical microscopy.
}
} The suggestions so far are: (a) light etching of a polished surface,
} followed by examination in a FESEM; or (b) preparation of an ion beam
} thinned sample for TEM.
}
} We would welcome any other suggestions. For example, would EBSD work? And
} if we tried (a), is there a suitable etchant (dilute HF?) for this material?
}
}
} With thanks,
}
} Thor Bostrom

Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca




From daemon Wed Nov 22 10:56:33 2000



From: Richard Beanland +44 1327 356363 :      richard.beanland-at-marconi.com
Date: Wed, 22 Nov 2000 16:53:46 +0000 (GMT)
Subject: Radiography and acoustic microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello all,
I am looking at real-time X-ray radiography systems and scanning acoustic microscopes for evaluation of solder bonds in optoelectronic packages. If anyone knows of suppliers of such systems - or even better has one they like (or don't), I would really like to hear from you. This includes manufacturers too - I'm not sure I have a comprehensive list yet.

Many thanks indeed,

Richard

==============================================================
Richard Beanland,
Structural Analysis Lab,
Caswell Technology,
Caswell,
Towcester,
Northants NN12 8EQ

e-mail richard.beanland-at-marconi.com
Tel. +44 1327 356363
Fax. +44 1327 356398
==============================================================
"The information contained in this message is legally privileged and confidential information intended for the eyes of the individual or entity named above. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message is strictly prohibited. If you have received this message in error, please notify us immediately by telephone.
Caswell Technology is the trading name of Marconi Caswell Limited. Registered in London No. 3694360 Registered Office: One Bruton Street London W1X 8AQ. Holding Company: Marconi plc."







From daemon Wed Nov 22 11:35:03 2000



From: James Roberts :      James.Roberts-at-mail.co.ventura.ca.us
Date: Wed, 22 Nov 2000 09:29:45 -0800
Subject: Re: Has anyone out there used an AO comparison microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


It depends on who's bridge you have. The two most common are the Leitz and the AO design. An AO type would say AO, Reichart or Leica on the manufactures logo depending on how new or old it is. Cambidge decided to use the Leica name on both once they owned both designs so the Leitz could also say Leica. There are other designs but these two make up probably more than 90% of all comparison bridges. I haven't worked with any of the others in over 20 years and then only a bit.

The knob in the center of the front makes it sound more likely to be an AO type. If it is, that knob should move the center prism set so that with it turned fully clock-wise you would see all of the left scope and turned fully counter-clock-wise you would see all of the right scope. With the knob centered you would see half of each field. In some other position you would see a smaller part of one field and a larger part of the other. If it is an AO style bridge it sounds to me as if the rack and gear might be damaged so the prism is not moving. The thin dividing line you see between the two fields should move as you turn the knob to give you a smaller percentage of one field and a larger percentage of the other. This lets us compare the pattern of stria over a length of the toolmark, a bullet comparison is a type of toolmark comparison. Side by side comparison is much more useful in Firearm and toolmark comparison than overlapping fields are.

If it is a Leitz design some do not let you move the dividing line off center but have other knobs that overlap the two fields or let you view just one. These bench top Leitz bridges don't have a knob in the center of the front so this probably isn't what you have.

In modern bridges the bridges that attach to separate scopes are designed for hair and fiber comparisons and the like. Bullet ("Universal Forensic") scopes have the objectives attached into the bridges in scopes that have been built since well before I entered the field over a 1/4 century ago. They are designed for much lower power and much longer working distances. The bridges for hair and fiber comparison scopes (yes they can be used for all manor of comparison but in forensic science labs that is their primary job and the way they are usually refereed to) are quite similar to bullet scope bridge designs but are designed to be attached to separate scopes that are basically conventional compound microscopes with sub-stage lighting.

If you tell me more about the bridge you are trying to use I may be able to tell you more that can help you set it up and see if it is working properly. AO then Reichart and now Leica have always said that users should not open up bridges to try repairs, as prism alignment is critical and easily disturbed. Rebuilds are available basically only at the factory, no one else has the alignment equipment to do it right. When other places have told me they could rebuild one I found that their version of a rebuild amounted to a real good cleaning job not prism replacement or realignment. If you do have an AO type and the knob is not moving the rack the center prism is on you may need such a rebuild. I did have a knob set screw come loose on an old one quite a number of years ago. I got away with going inside and tightening it up but I would not recommend it to anyone else as these are expensive pieces of equipment and rebuilds are several thousand dollars (around 12K to 15K last time I checked several years ago).

Jim


James L. Roberts
Firearm and Toolmark Examiner
Ventura Co. Sheriff's Lab
(805) 654-2308
James.Roberts-at-mail.co.ventura.ca.us

} } } {"COURYHOUSE-at-aol.com"-at-sparc5.microscopy.com} 11/21/00 10:43PM } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Ahoy fellow enthusiasts!
Has anyone out there used a comparison microscope before?
I got a bridge unit and dropped it on top of 2 AO 10 scopes, there seems to
me a knob on the front that does nothing. I get one scope in once side of
the field and the image from the other scope on the other side.....

I suspect the knob makes them overlap (like we have seen on the police
shows but it does not seem to move the image.
when they compare bullets...)

any input?

thanks Ed Sharpe archivist for SMECC









From daemon Wed Nov 22 11:52:39 2000



From: James Roberts :      James.Roberts-at-mail.co.ventura.ca.us
Date: Wed, 22 Nov 2000 09:48:38 -0800
Subject: Re: Has anyone out there used an AO comparison microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Well I guess I should read the subject line as well as the body of the note and I would have seen that it was an AO bridge. Sorry for the info on the Leitz design. As indicated in my earlier message the knob should move the prism set so that you see part of one field and part of the other and what percentage of each is determined by the position of the knob. In the AO they do not "overlap."

If the knob is not moving the prism set you may have a loose set screw or you may need a bridge rebuild. Again I would not recommend going into the bridge yourself as alignment of the left, right and center prism is critical.
Jim

James L. Roberts
Firearm and Toolmark Examiner
Ventura Co. Sheriff's Lab
(805) 654-2308
James.Roberts-at-mail.co.ventura.ca.us

} } } {"COURYHOUSE-at-aol.com"-at-sparc5.microscopy.com} 11/21/00 10:43PM } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Ahoy fellow enthusiasts!
Has anyone out there used a comparison microscope before?
I got a bridge unit and dropped it on top of 2 AO 10 scopes, there seems to
me a knob on the front that does nothing. I get one scope in once side of
the field and the image from the other scope on the other side.....

I suspect the knob makes them overlap (like we have seen on the police
shows but it does not seem to move the image.
when they compare bullets...)

any input?

thanks Ed Sharpe archivist for SMECC










From daemon Wed Nov 22 13:03:16 2000



From: Gary Gaugler :      gary-at-gaugler.com
Date: Wed, 22 Nov 2000 16:24:58 -0800
Subject: SEM resolution calibration methods

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Mary,

I have been waiting for a response from your technical support concerning
mapping . Can you have them call me.

Thank you
Larry Howard




} From: "Mary Mager" {mager-at-interchange.ubc.ca}
To: "Kelloes, Cathy L" {KELLOECL-at-bp.com}
Cc: {Microscopy-at-sparc5.microscopy.com}
Sent: Tuesday, November 21, 2000 1:07 PM


NIST used to offer a SEM calibration standard SRM-2069.
I don't see this any longer. If one needs to calibrate or
qualify a SEM's resolution at 250A or better, what is
an appropriate method?

I've used Au on C "standards" but these are more
qualitative than quantitative. The question is how to
make a quantitative measurement of SEM resolution?

Vendor responses are welcome.

Thanks,
gary g.




From daemon Wed Nov 22 19:22:36 2000



From: Dave Audette :      deaudette-at-yahoo.com
Date: Wed, 22 Nov 2000 17:17:13 -0800 (PST)
Subject: UNIX based EDS system

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


List Members,

Earlier this month I requested information to help
network an EDS system, which is a UNIX based system
and operates on a Sun Sparc Station 5 with SunOS 5.3.
I really appreciate all the info people have sent me
and I wanted to let you know that the system has been
placed on the network (TCPIP). The local IT people
suggested I use an outside expert, so we did arrange
for an ex-Sun employee to come in and do the job. It
did take, as one suggested, 15 minutes to place the
Sun on the network, but it did take another hour to
(re)write some missing files (not his exact words),
which allows someone on the pc network (me) to be able
to ftp or take files from the Sun and bring to the pc
environment. It was worth the work as we don't have
to scan in the data for our reports etc. So the
process does work and is pretty simple, but later I
may explore some of the Windows programs suggested to
facilitate these file transfers in a drag and drop
style.

Thanks again,

Dave Audette
david.audette-at-sylvania.com


__________________________________________________
Do You Yahoo!?
Yahoo! Shopping - Thousands of Stores. Millions of Products.
http://shopping.yahoo.com/



From daemon Wed Nov 22 20:34:08 2000



From: Gary Gaugler :      gary-at-gaugler.com
Date: Wed, 22 Nov 2000 18:29:26 -0800
Subject: Re: Does Mary Mager work for Quartz PCI or University of

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Looking at her return address:

mager-at-interchange.ubc.ca

suggests to me that she is with the University of British Columbia,
Canada and not part of Quartz/Hitachi. Are you having trouble
with her or Quartz/Hitachi?

gary g.


At 10:58 AM 11/22/00, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From daemon Thu Nov 23 03:41:16 2000



From: Chris Jeffree :      cjeffree-at-srv0.bio.ed.ac.uk
Date: Thu, 23 Nov 2000 09:27:52 -0000
Subject: Re: SEM resolution calibration methods

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Gary
I agree with you that an objective test is hard to come by and that
an industry-standard approach is lacking. I make the following
observations, for what they're worth:

There is no straightforward way of making a specimen that tests the
resolution of all SEMs, under all operating conditions and for all
specimen types. Gold on carbon is the best choice if that is what
you look at for a living. If you look at fine carbon structures on
carbon it's a poor test of practical resolution, although it may
indicate the general health of the instrument.

Makers differ in their approaches to resolution testing. Most employ
gold on carbon, but sometimes gold on magnetic tape or dendritic
crystalline gold or silver is preferred. Some measure the smallest
particles visible, others measure the smallest gaps between larger
particles. One practical difficulty in the former approach is that the
particles need to be most abundant in a size range close to the
dimensions you want to test for. SEM resolutions span at least an
order of magnitude from {0.5nm for in-lens fegsem to } 5nm for
tungsten sem, making production of a single universal specimen
difficult. At the limits of FEGSEM resolution, ~1nm, suitably small
gold particles on carbon or mag tape can be as rare as hen's teeth.
Another issue is that at the limit of an instrument's resolution the
image of a small particle has a bell-shaped gaussian distribution of
signal, fading imperceptibly into the background. The problem here
is to determine where the edges of the feature are located. Pixel
size is relevant here. If the feature is only two or three pixels wide,
typical of a x100k image at 1kx1k pixels, then pixel size has a
dominant effect on the measured size increments - ten pixels wide
or more begins to yield an approximation to continuous data, so the
image size must be large or the magnification high. The standard
test of resolution in microanalysis, where peaks are again
approximately normal curves, is to measure peak width at half
height, and one can approximate this approach in measuring a gold
particle by expanding the image contrast until local background is 0
and max signal is 255, setting the threshold to 127 and measuring
mean feret diameter of the feature. I think you'll find that if you
apply this type of criterion you will get a more conservative estimate
of resolution than is claimed by the manufacturer.

A variant of gold on carbon which we have tried is to deposit 1
nanometre gold colloid onto formvar/carbon, with 10nm gold to
provide something to focus on. This is a specimen that can be
characterised by TEM before examination in the SEM, so you can
determine what the actual particle size distribution is (difficult to do
that with gold on mag tape). FEGSEMs can "see" the 1nm gold
particles because there is strong signal against low background, but
as in light microscopy, where 25nm colloidal gold is readily seen
under reflected illumination, may image them to a larger diameter.
This larger diameter, measured using peak width at half height, thus
constitutes an estimate of instrument resolving power. Another
approach in principle (harder in practice) is to create perfectly
square, perfectly nanometre-sharp square edges in a perfectly
smooth surface of the element of your choice - silicon e.g., or a
layer of another element deposited thereon - and to measure the
peak width at half height of the edge transition.

Finally, theoretically it is possiible objectively to determine the
limiting dimensions recorded in any image by looking at its fourier
power spectrum. Biomolecular structure people regularly report
such data for TEM images, so presumably the technology is
availalble. Any observations on where, how, and the limitations of
applying it to sem resolution measurment would be gratefully
received.

Best wishes
Chris

} NIST used to offer a SEM calibration standard SRM-2069.
} I don't see this any longer. If one needs to calibrate or
} qualify a SEM's resolution at 250A or better, what is
} an appropriate method?
}
} I've used Au on C "standards" but these are more
} qualitative than quantitative. The question is how to
} make a quantitative measurement of SEM resolution?
}
} Vendor responses are welcome.
}
} Thanks,
} gary g.
}
}


~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Dr Chris Jeffree
University of Edinburgh
Biological Sciences EM Facility
Daniel Rutherford Building
King's Buildings EDINBURGH EH9 3JH
Tel: +44 (0) 131 650 5345
FAX: +44 (0) 131 650 6563

Inveresk Cottage, 26 Carberry Road,
Inveresk, Musselburgh, Midlothian EH21 8PR, UK
Tel: +44 (0) 131 665 6062 / Mobile 07710 585 401
FAX: +44 (0) 131 653 6248
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~



From daemon Thu Nov 23 03:41:16 2000



From: Chris Jeffree :      cjeffree-at-srv0.bio.ed.ac.uk
Date: Thu, 23 Nov 2000 09:23:57 -0000
Subject: Re: SEM resolution calibration methods

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Gary
I agree with you that an objective test is hard to come by and that
an industry-standard approach is lacking. I make the following
observations, for what they're worth:

There is no straightforward way of making a specimen that tests the
resolution of all SEMs, under all operating conditions and for all
specimen types. Gold on carbon is the best choice if that is what
you look at for a living. If you look at fine carbon structures on
carbon it's a poor test of practical resolution, although it may
indicate the general health of the instrument.

Makers differ in their approaches to resolution testing. Most employ
gold on carbon, but sometimes gold on magnetic tape or dendritic
crystalline gold or silver is preferred. Some measure the smallest
particles visible, others measure the smallest gaps between larger
particles. One practical difficulty in the former approach is that the
particles need to be most abundant in a size range close to the
dimensions you want to test for. SEM resolutions span at least an
order of magnitude from {0.5nm for in-lens fegsem to } 5nm for
tungsten sem, making production of a single universal specimen
difficult. At the limits of FEGSEM resolution, ~1nm, suitably small
gold particles on carbon or mag tape can be as rare as hen's teeth.
Another issue is that at the limit of an instrument's resolution the
image of a small particle has a bell-shaped gaussian distribution of
signal, fading imperceptibly into the background. The problem here
is to determine where the edges of the feature are located. Pixel
size is relevant here. If the feature is only two or three pixels wide,
typical of a x100k image at 1kx1k pixels, then pixel size has a
dominant effect on the measured size increments - ten pixels wide
or more begins to yield an approximation to continuous data, so the
image size must be large or the magnification high. The standard
test of resolution in microanalysis, where peaks are again
approximately normal curves, is to measure peak width at half
height, and one can approximate this approach in measuring a gold
particle by expanding the image contrast until local background is 0
and max signal is 255, setting the threshold to 127 and measuring
mean feret diameter of the feature. I think you'll find that if you
apply this type of criterion you will get a more conservative estimate
of resolution than is claimed by the manufacturer.

A variant of gold on carbon which we have tried is to deposit 1
nanometre gold colloid onto formvar/carbon, with 10nm gold to
provide something to focus on. This is a specimen that can be
characterised by TEM before examination in the SEM, so you can
determine what the actual particle size distribution is (difficult to do
that with gold on mag tape). FEGSEMs can "see" the 1nm gold
particles because there is strong signal against low background, but
as in light microscopy, where 25nm colloidal gold is readily seen
under reflected illumination, may image them to a larger diameter.
This larger diameter, measured using peak width at half height, thus
constitutes an estimate of instrument resolving power. Another
approach in principle (harder in practice) is to create perfectly
square, perfectly nanometre-sharp square edges in a perfectly
smooth surface of the element of your choice - silicon e.g., or a
layer of another element deposited thereon - and to measure the
peak width at half height of the edge transition.

Finally, theoretically it is possiible objectively to determine the
limiting dimensions recorded in any image by looking at its fourier
power spectrum. Biomolecular structure people regularly report
such data for TEM images, so presumably the technology is
availalble. Any observations on where, how, and the limitations of
applying it to sem resolution measurment would be gratefully
received.

Best wishes
Chris

} NIST used to offer a SEM calibration standard SRM-2069.
} I don't see this any longer. If one needs to calibrate or
} qualify a SEM's resolution at 250A or better, what is
} an appropriate method?
}
} I've used Au on C "standards" but these are more
} qualitative than quantitative. The question is how to
} make a quantitative measurement of SEM resolution?
}
} Vendor responses are welcome.
}
} Thanks,
} gary g.
}
}


~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Dr Chris Jeffree
University of Edinburgh
Biological Sciences EM Facility
Daniel Rutherford Building
King's Buildings EDINBURGH EH9 3JH
Tel: +44 (0) 131 650 5345
FAX: +44 (0) 131 650 6563

Inveresk Cottage, 26 Carberry Road,
Inveresk, Musselburgh, Midlothian EH21 8PR, UK
Tel: +44 (0) 131 665 6062 / Mobile 07710 585 401
FAX: +44 (0) 131 653 6248
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~



From daemon Thu Nov 23 03:43:33 2000



From: Aviles, Phil :      paviles-at-dps.state.nm.us
Date: Wed, 22 Nov 2000 16:53:48 -0700
Subject: RE: Has anyone out there used an AO comparison microscope before?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The knob on the front will not affect the image, just move across the field
to expose a portion or all of either the right or left image. This is used
in searching the surface areas of bullets or tool marks in forensic
comparisons. You're doing it right so far. Have fun.

Phil Aviles
Forensic Microanalyst

} -----Original Message-----
} From: "COURYHOUSE-at-aol.com"-at-sparc5.microscopy.com
} [SMTP:"COURYHOUSE-at-aol.com"-at-sparc5.microscopy.com]
} Sent: Tuesday, November 21, 2000 11:43 PM
} To: MICROSCOPY-at-sparc5.microscopy.com
} Subject: Has anyone out there used an AO comparison microscope
} before?
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Ahoy fellow enthusiasts!
} Has anyone out there used a comparison microscope before?
} I got a bridge unit and dropped it on top of 2 AO 10 scopes, there seems
} to
} me a knob on the front that does nothing. I get one scope in once side of
} the field and the image from the other scope on the other side.....
}
} I suspect the knob makes them overlap (like we have seen on the police
} shows but it does not seem to move the image.
} when they compare bullets...)
}
} any input?
}
} thanks Ed Sharpe archivist for SMECC
}
}
}



From daemon Thu Nov 23 07:10:25 2000



From: Richard M Langford :      richard.langford-at-materials.oxford.ac.uk
Date: Thu, 23 Nov 2000 13:03:30 -0000
Subject: FIB User List

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear All

A discussion list has been set up for focused ion beam users.

The subscribing /archive page is at:

http://www.jiscmail.ac.uk/lists/FIB-USERS.html

Regards

Richard

--------------------------------------------------------------
Richard M Langford

Department of Materials, University of Oxford
Parks Road, Oxford, OX1 3PH, UK

Tel: +44 (0)1865 273734, Fax: +44 (0)1865 273789
email: richard.langford-at-materials.oxford.ac.uk





From daemon Thu Nov 23 11:00:28 2000



From: Lhconsulting-at-aol.com
Date: Thu, 23 Nov 2000 11:50:51 EST
Subject: Re: Does Mary Mager work for Quartz PCI or University of British Columbia

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thank you for the clarification, but im still confused, your business card
states that you are the EDX Product Development person. then would you be
able to answer my questions? Why is it I cant get an answer about
imaging/elemental mapping. ( See attached email)

Larry Howard


In a message dated 11/22/00 11:04:36 PM Eastern Standard Time,
mmager-at-qrtz.com writes:

{ { Dear Larry,

I'm sorry if our technical support team did not respond to your request for
information on our mapping option. The passive, full position-tagged mapping
option is just now finished and the team is working very hard to get the
information out to all the customers that requested it. I will be happy to
phone you and answer any questions that you may have, if you will send me
your
phone number by return e-mail.

In answer to the subject of your e-mail, I work as a microscopist for the
University of British Columbia and conduct any correspondance with the MSA
Listserver in that capacity. I am also
an EDX technical consultant for the X-ray Division of Quartz Imaging Corp. I
have little to do with the Quartz PCI product except as a user and friend of
the owner of Quartz.At 01:58 PM 11/22/00 -0500, you wrote:
} Mary,
}
} I have been waiting for a response from your technical support concerning
} mapping . Can you have them call me.
}
} Thank you
} Larry Howard

Best regards,
Mary


Mary Mager
EDX Product Specialist
Quartz Imaging Corp.
810 - 1112 West Pender Street
Vancouver, B.C.
Canada V6E 3X5
tel: 604-488-3911
fax: 604-488-3922
e-mail: mmager-at-qrtz.com
--------------------

Dear Larry,

I'm sorry if our technical support team did not respond to your request for
information on our mapping option. The passive, full position-tagged mapping
option is just now finished and the team is working very hard to get the
information out to all the customers that requested it. I will be happy to
phone you and answer any questions that you may have, if youwill send me your
phone number by return e-mail.

In answer to the subject of your e-mail, I work as a microscopist for the
University of British Columbia and conduct any correspondance with theMSA
Listserver in that capacity. I am also
an EDX technical consultant for the X-ray Division of Quartz Imaging Corp. I
have little to do with the Quartz PCI product except as a userand friend of
the owner of Quartz.At 01:58 PM 11/22/00 -0500, youwrote:
} Mary,
}
} I have been waiting for a response from your technical supportconcerning
} mapping . Can you have them call me.
}
} Thank you
} Larry Howard

Best regards,
Mary


Mary Mager
EDX Product Specialist
Quartz Imaging Corp.
810 - 1112 West Pender Street
Vancouver, B.C.
Canada V6E 3X5
tel: 604-488-3911
fax: 604-488-3922
e-mail: mmager-at-qrtz.com


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From daemon Thu Nov 23 11:39:13 2000



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Thu, 23 Nov 2000 10:31:16 -0700
Subject: Re: SEM resolution calibration methods

Contents Retrieved from Microscopy Listserver Archives
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Chris,

the way people with TEMs measure the resolution is pretty straight forward:
They take an image and do a Fourier transform (either through software FFT
or with an optical diffractometer). The FFT then shows essentially the
frequencies that are on the image. For a TEM you can also calculate the
Contrast Transfer Function (CTF), which essentially gives you the same
information. By comparing the two, you can thus find out, what the
resolution of the microscope is. Since the CTF is a fairly complex function,
there are different "resolutions": Point resolution (corresponds to the
frequency where the CTF becomes zero the first time), or the "lattice
resolution" (where the intensity of the oscillations goes below a certain
threshold).

For an SEM, I assume there is only a gradually declining FFT (Intensity vs.
spatial frequency). Unless someone "defines" where the cut-off is (for
example where the intensity has gone down to 20%), I don't think you can
really determine the resolution with certainty. The sample also needs to be
defined (for example a sharp metal edge, as any charging may affect the
resolution. Also the material itself may have to be defined, as edge effects
may influence the measurement. Finally, the sample must show structure below
the resolution limit of the microscope, otherwise you measure the
"resolution" of your sample. In TEMs that is fairly simple: use an amorphous
material. For SEM samples I don't know what could be used. Something with
atomic structure on the surface? A surface reconstructed semiconductor?



Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Chris Jeffree [mailto:cjeffree-at-srv0.bio.ed.ac.uk]
Sent: Thursday, November 23, 2000 2:24 AM
To: Gary Gaugler
Cc: microscopy-at-sparc5.microscopy.com


Gary
I agree with you that an objective test is hard to come by and that
an industry-standard approach is lacking. I make the following
observations, for what they're worth:

There is no straightforward way of making a specimen that tests the
resolution of all SEMs, under all operating conditions and for all
specimen types. Gold on carbon is the best choice if that is what
you look at for a living. If you look at fine carbon structures on
carbon it's a poor test of practical resolution, although it may
indicate the general health of the instrument.

Makers differ in their approaches to resolution testing. Most employ
gold on carbon, but sometimes gold on magnetic tape or dendritic
crystalline gold or silver is preferred. Some measure the smallest
particles visible, others measure the smallest gaps between larger
particles. One practical difficulty in the former approach is that the
particles need to be most abundant in a size range close to the
dimensions you want to test for. SEM resolutions span at least an
order of magnitude from {0.5nm for in-lens fegsem to } 5nm for
tungsten sem, making production of a single universal specimen
difficult. At the limits of FEGSEM resolution, ~1nm, suitably small
gold particles on carbon or mag tape can be as rare as hen's teeth.
Another issue is that at the limit of an instrument's resolution the
image of a small particle has a bell-shaped gaussian distribution of
signal, fading imperceptibly into the background. The problem here
is to determine where the edges of the feature are located. Pixel
size is relevant here. If the feature is only two or three pixels wide,
typical of a x100k image at 1kx1k pixels, then pixel size has a
dominant effect on the measured size increments - ten pixels wide
or more begins to yield an approximation to continuous data, so the
image size must be large or the magnification high. The standard
test of resolution in microanalysis, where peaks are again
approximately normal curves, is to measure peak width at half
height, and one can approximate this approach in measuring a gold
particle by expanding the image contrast until local background is 0
and max signal is 255, setting the threshold to 127 and measuring
mean feret diameter of the feature. I think you'll find that if you
apply this type of criterion you will get a more conservative estimate
of resolution than is claimed by the manufacturer.

A variant of gold on carbon which we have tried is to deposit 1
nanometre gold colloid onto formvar/carbon, with 10nm gold to
provide something to focus on. This is a specimen that can be
characterised by TEM before examination in the SEM, so you can
determine what the actual particle size distribution is (difficult to do
that with gold on mag tape). FEGSEMs can "see" the 1nm gold
particles because there is strong signal against low background, but
as in light microscopy, where 25nm colloidal gold is readily seen
under reflected illumination, may image them to a larger diameter.
This larger diameter, measured using peak width at half height, thus
constitutes an estimate of instrument resolving power. Another
approach in principle (harder in practice) is to create perfectly
square, perfectly nanometre-sharp square edges in a perfectly
smooth surface of the element of your choice - silicon e.g., or a
layer of another element deposited thereon - and to measure the
peak width at half height of the edge transition.

Finally, theoretically it is possiible objectively to determine the
limiting dimensions recorded in any image by looking at its fourier
power spectrum. Biomolecular structure people regularly report
such data for TEM images, so presumably the technology is
availalble. Any observations on where, how, and the limitations of
applying it to sem resolution measurment would be gratefully
received.

Best wishes
Chris

} NIST used to offer a SEM calibration standard SRM-2069.
} I don't see this any longer. If one needs to calibrate or
} qualify a SEM's resolution at 250A or better, what is
} an appropriate method?
}
} I've used Au on C "standards" but these are more
} qualitative than quantitative. The question is how to
} make a quantitative measurement of SEM resolution?
}
} Vendor responses are welcome.
}
} Thanks,
} gary g.
}
}


~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Dr Chris Jeffree
University of Edinburgh
Biological Sciences EM Facility
Daniel Rutherford Building
King's Buildings EDINBURGH EH9 3JH
Tel: +44 (0) 131 650 5345
FAX: +44 (0) 131 650 6563

Inveresk Cottage, 26 Carberry Road,
Inveresk, Musselburgh, Midlothian EH21 8PR, UK
Tel: +44 (0) 131 665 6062 / Mobile 07710 585 401
FAX: +44 (0) 131 653 6248
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~



From daemon Thu Nov 23 13:37:20 2000



From: Grizzi Fabio ICH :      fabio.grizzi-at-humanitas.it
Date: Thu, 23 Nov 2000 20:32:51 +0100
Subject: immunohistochemistry

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear All

I would like to ask you how it is possible to identify the inflammatory
cells in paraffin embedded human liver specimens by means of
immunohistochemistry technique. Is there a better antibody?

Many thanks

Fabio Grizzi

Dott. Fabio Grizzi
Istituto Clinico Humanitas - Scientific Direction
Via Manzoni 56, Rozzano, Milan, Italy
Tel. ++390282244548
Fax ++390282244590
E-mail fabio.grizzi-at-humanitas.it {mailto:fabio.grizzi-at-humanitas.it}





From daemon Thu Nov 23 18:59:03 2000



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Fri, 24 Nov 2000 13:58:47 GMT+1200
Subject: Re: Does Mary Mager work for Quartz PCI or University of British

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I don't see why this is being conducted on the list.

Can't you guys sort it out in private?

cheers

rtch

Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand



From daemon Thu Nov 23 18:59:08 2000



From: COURYHOUSE-at-aol.com
Date: Thu, 23 Nov 2000 19:52:20 EST
Subject: UPDATE: Has anyone out there used an AO comparison microscope before?

Contents Retrieved from Microscopy Listserver Archives
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Thanks to James and all others that replied and helped shed some light on
this matter

What it appears we have is bridge for an AO hair and fiber comparison scope.
It seems to be fixed in the split mode, which is fine, as it will show a
half for each scope. We have 2 model 10 AO scopes in the Museum's parts
stash and I put enough stuff together to make 2 with the exception that I
am missing the parts for both of the mechanical stages... Any one have some
AO carcasses out there we can raid for parts?

thanks Ed Sharpe archivist for SMECC


{ { Subj: Re: Has anyone out there used an AO comparison microscope before?
Date: 11/22/00 1:33:22 PM US Mountain Standard Time
From: James.Roberts-at-mail.co.ventura.ca.us (James Roberts)
To: COURYHOUSE-at-sparc5.microscopy.com, MICROSCOPY-at-sparc5.microscopy.com

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It depends on who's bridge you have. The two most common are the Leitz and
the AO design. An AO type would say AO, Reichart or Leica on the
manufactures logo depending on how new or old it is. Cambidge decided to use
the Leica name on both once they owned both designs so the Leitz could also
say Leica. There are other designs but these two make up probably more than
90% of all comparison bridges. I haven't worked with any of the others in
over 20 years and then only a bit.

The knob in the center of the front makes it sound more likely to be an AO
type. If it is, that knob should move the center prism set so that with it
turned fully clock-wise you would see all of the left scope and turned fully
counter-clock-wise you would see all of the right scope. With the knob
centered you would see half of each field. In some other position you would
see a smaller part of one field and a larger part of the other. If it is an
AO style bridge it sounds to me as if the rack and gear might be damaged so
the prism is not moving. The thin dividing line you see between the two
fields should move as you turn the knob to give you a smaller percentage of
one field and a larger percentage of the other. This lets us compare the
pattern of stria over a length of the toolmark, a bullet comparison is a type
of toolmark comparison. Side by side comparison is much more useful in
Firearm and toolmark comparison than overlapping fields are.

If it is a Leitz design some do not let you move the dividing line off
center but have other knobs that overlap the two fields or let you view just
one. These bench top Leitz bridges don't have a knob in the center of the
front so this probably isn't what you have.

In modern bridges the bridges that attach to separate scopes are designed
for hair and fiber comparisons and the like. Bullet ("Universal Forensic")
scopes have the objectives attached into the bridges in scopes that have been
built since well before I entered the field over a 1/4 century ago. They are
designed for much lower power and much longer working distances. The bridges
for hair and fiber comparison scopes (yes they can be used for all manor of
comparison but in forensic science labs that is their primary job and the way
they are usually refereed to) are quite similar to bullet scope bridge
designs but are designed to be attached to separate scopes that are basically
conventional compound microscopes with sub-stage lighting.

If you tell me more about the bridge you are trying to use I may be able to
tell you more that can help you set it up and see if it is working properly.
AO then Reichart and now Leica have always said that users should not open up
bridges to try repairs, as prism alignment is critical and easily disturbed.
Rebuilds are available basically only at the factory, no one else has the
alignment equipment to do it right. When other places have told me they
could rebuild one I found that their version of a rebuild amounted to a real
good cleaning job not prism replacement or realignment. If you do have an AO
type and the knob is not moving the rack the center prism is on you may need
such a rebuild. I did have a knob set screw come loose on an old one quite
a number of years ago. I got away with going inside and tightening it up but
I would not recommend it to anyone else as these are expensive pieces of
equipment and rebuilds are several thousand dollars (around 12K to 15K la!
st time I checked several years ago).

Jim


James L. Roberts
Firearm and Toolmark Examiner
Ventura Co. Sheriff's Lab
(805) 654-2308
James.Roberts-at-mail.co.ventura.ca.us

} } } {"COURYHOUSE-at-aol.com"-at-sparc5.microscopy.com} 11/21/00 10:43PM } } }
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Ahoy fellow enthusiasts!
Has anyone out there used a comparison microscope before?
I got a bridge unit and dropped it on top of 2 AO 10 scopes, there seems to
me a knob on the front that does nothing. I get one scope in once side of
the field and the image from the other scope on the other side.....

I suspect the knob makes them overlap (like we have seen on the police
shows but it does not seem to move the image.
when they compare bullets...)

any input?

thanks Ed Sharpe archivist for SMECC


} }



From daemon Thu Nov 23 21:09:40 2000



From: steven wintonick :      crimsem-at-hotmail.com
Date: Thu, 23 Nov 2000 22:03:06 -0500
Subject: SEM/EDS - Explosives

Contents Retrieved from Microscopy Listserver Archives
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Hello All,

Looking for some information on references (websites,books,journals, etc.)
and/or SOPs/Procedures for the analysis of explosives using SEM/EDS. Thanks.

Steve Wintonick
_____________________________________________________________________________________
Get more from the Web. FREE MSN Explorer download : http://explorer.msn.com




From daemon Thu Nov 23 21:51:11 2000



From: Sally Stowe :      STOWE-at-rsbs.anu.edu.au
Date: Fri, 24 Nov 2000 14:46:23 +1100
Subject: Australian Workshop on Nanotubes and Fullerenes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} From Ying Chen, ying.chen-at-anu.edu.au

First Announcement

Australian Workshop on Nanotubes and Fullerenes

May 3-4, 2001

Australian National University, Canberra


Scope
Fullerenes, nanotubes and related nanomaterials are receiving great
interest due to the new mechanical, physical and chemical properties and
potential applications in various areas. A workshop is designed to bring
together research scientists and engineers working in various disciplines
in the broad area of nanotube and Fullerene -related materials and to
provide an opportunity to exchange new ideas and results. Postgraduate
students are particularly encouraged to present their thesis work.

Program
The program consists of invited lectures from both Australian and
international leading scientists as well as oral and poster contributions.

It will be divided into the following sessions (but not limited to):

Thermodynamics and Modeling
Synthesis and Processing
Characterization
Properties and Applications

Partial list of invited speakers

Professor David Tomanek, Michigan State University, USA

Professor H. M. Cheng
Institute of Metal Research, Chinese Academy of Science, China
Title: Carbon nanotube synthesis and hydrogen storage

Professor M. Wilson, University of Technology, Sydney
Title: Changing the diameter of carbon nanotubes

Professor G. Wallace, University of Wollongong
Title: Carbon Nanotube/Conducting polymer Composites

Dr. L.M. Dai, Molecular Science, CSIRO
Title:Controlled growth and surface modification of carbon nanotubes

Venue
The workshop will be held in the seminar room of the link building (58) of

the Research School of Physical Science and Engineering, Mills Road, The
Australian National University, Canberra, Australia

Abstract submission
Please send your abstracts and registration form by email attachment to
awnf2001-at-anu.edu.au Acceptance and presentation type (invited, oral or
poster) will be informed by e-mail. Example of abstract format and
registration forms can be found at:
http://www.rsphysse.anu.edu.au/nanotube/awnf2001/

Correspondence
Dr Ying Chen
Department of Engineering, FEIT
&Department of Electronic Materials Engineering
Research School of Physical Science and Engineering
Australian National University
Canberra, ACT 0200
Australia
E-mail: ying.chen-at-anu.edu.au, or awnf2001-at-anu.edu.au
Telephone: 61 02 6249 0380, ( 6125 0380 after 31-12-2000)
Fax: 61 02 6279 8338 ( 6125 6279 after 31-12-2000)

PhD scholarships on nanotube research are available at ANU and information

can be found at
group webpage: http://www.rsphysse.anu.edu.au/nanotube/


Dr Sally Stowe
Facility Coordinator, ANU EMU
Box 475 Canberra ACT 2601
AUSTRALIA
stowe-at-rsbs.anu.edu.au fax 61 (0)2 6249 3218 or 6279 8525



From daemon Fri Nov 24 01:47:01 2000



From: Gareth Morgan :      Gareth.Morgan-at-impi.ki.se
Date: Fri, 24 Nov 2000 08:48:30 +0100
Subject: Re: immunohistochemistry

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi

Yes it is very possible to identify such cells. Do you want to identify all
cells that might be involved in the inflammatory/immune response or subsets
of these. In the first case you could use a broad spectrum antibody to the
'leukocyte common antigen/s' CD45 family or if you want to identify the
subcategories eg macrophages, neutrophils, T/B lymphocytes etc then there
is also a wide range available.

If you want further details of supply companies mail back to me and I can
send you some web addresses - I have no connection to any companies - I am
just a user.

Have you talked to your local hospital pathology department?



At 20:32 2000-11-23 +0100, Grizzi Fabio ICH wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Med vänliga hälsningar/With best regards

Gareth

e-mail Gareth.Morgan-at-impi.ki.se

http://www.ki.se/biomedlab/index_se.html

Tel +46 8 728 3734
Fax +46 8 728 3688

"Words are the seeds of misunderstanding - use them carefully."

"Give us the wisdom to know and not to feel that not knowing is less than
wisdom itself."

Gareth Morgan MPhil MSc FIBMS,
Institutionen för Mikrobiologi, Patologi och Immunologi(IMPI), H5,
Karolinska Institutet,
Avdelningar för biomedicinsk
laboratorievetenskap och
biomedicinska ämnen,
Box 127 73,
S 112 96, Stockholm
Sverige

NB/Obs! Visiting address =

Lindhagensgatan 92
Kungsholmen



From daemon Fri Nov 24 02:29:04 2000



From: Tim E. Harper :      tim-at-cmp-cientifica.com
Date: Fri, 24 Nov 2000 11:49:38 +0100
Subject: NanoTechnology Weekly

Contents Retrieved from Microscopy Listserver Archives
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Mike
What software do you recommend for this? Does AnalySIS have
this capability?
Chris

} From: Mike Bode {mb-at-Soft-Imaging.com}
To: "'Microscopy-at-MSA.Microscopy.Com'" {Microscopy-at-sparc5.microscopy.com}


Dear All,

TNT (Trends in Nanotechnology) Weekly, a free, weekly e-mail round up of
happenings in the world of nanotechnology will be launched on 5th December
2000. More information and a sample issue can be found at www.cientifica.com

If anyone on the list would like to contribute, or comment on the
newsletter, or indeed help us to enhance is usefulness to the microscopy
community, please contact me directly.

Regards

Tim

*****************************************************************
Tim E. Harper Managing Director
CMP Cientifica s.l.
Space & NanoTechnology Division
Phone +34 91 640 71 85 Fax +34 91 640 71 86
http://www.cmp-cientifica.com/





From daemon Fri Nov 24 12:41:23 2000



From: tsr :      tsr-at-codon.nih.gov
Date: Fri, 24 Nov 2000 13:37:20 -0800
Subject: June Mahs seminar

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Wondered if you could send me information on above. My address is:
10605 Belfast Place, Potomac, MD 20854 (or by return
e-mail)...thanks!...Tom Reese



From daemon Sun Nov 26 13:25:25 2000



From: eActive.com :      confirm-at-EACTIVE.COM
Date: Thu, 16 Nov 2000 10:34:00 -0500
Subject: eActive.com - Confirmation message

Contents Retrieved from Microscopy Listserver Archives
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Hello,

Your email address is part of an email list acquired by eActive, Inc.

Your name came up as being interested in receiving information
using Video, Streaming Media, mp3, Windows Media Player,
QuickTime, Real Media Player, Flash, Shock Wave, Audio and
other Interactive Multimedia Presentations and to be kept
informed of all upcoming unique Sales, Information, Contests
and Giveaways presented by eActive.

If you do not reply to this message your email address
will be taken off the list and you will not receive
Interactive Multimedia Presentations.

eActive Inc. is committed to ensuring your Internet privacy.
In order to be absolutely certain that you have agreed to
receive Multimedia Presentations via email we are asking
you to verify your email address.

Therefore:
1) Click this link: mailto:confirm-at-eactive.com?subject=OK
or
2) Reply to this email with "OK" in the subject line.

If you have received this email in error, DO NOT REPLY,
simply delete this email and your name will be purged
from our database. You will not be contacted again by eActive.

Thank you!

If you have any questions please contact us at: admin-at-eactive.com


-----

eActive Inc.,
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Suite 210
Delray Beach, FL 33484



From daemon Sun Nov 26 19:31:57 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Sun, 26 Nov 2000 19:26:03 -0600
Subject: Administrivia: eActive.com .... is SPAM Mail

Contents Retrieved from Microscopy Listserver Archives
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Colleagues....

A general message to answer a number questions I
have been receiving.

The eActive Mail dated Nov. 16th is SPAM/JUNK mail.
Neither MSA nor the Microscopy Listserver has sold
your address to anyone. I keep hold all addresses private
and do not disclose them to anyone.

The mail you all received was just a junk mailing, which
occassionally gets through the filters.

I have added eActive.Com to the SPAM filter so they
should not be bothering us again.

Cheers...
Nestor
Your Friendly Neighborhood SysOp.





From daemon Mon Nov 27 09:07:23 2000



From: chris smith (IACR-RES) :      chris.smith-at-bbsrc.ac.uk
Date: Mon, 27 Nov 2000 14:56:12 -0000
Subject: Edwards E306A

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Hi Jonathan,
I sent a preliminary message to your private e-mail address but got an
'undeliverable' note.
Given a working address I can send you a copy of my own instruction sheet,
photos of set up, etc. used weekly for 20 yrs with total success.
Please contact me direct on: chris.smith-at-bbsrc.ac.uk
EM Unit, IACR-Rothamsted, Harpenden, UK



From daemon Mon Nov 27 11:52:04 2000



From: Erdem Yasar :      yasar-at-turkuaz.kku.edu.tr
Date: Mon, 27 Nov 2000 19:43:34 +0200 (EET)
Subject: Re: ABOUT JSM5600 SEM AND JEM3010 TEM

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Dear Sir/Madam ,
JEM3010 was manufactured in 1999.it has got 1.500.000x mag and digital
camera system JSM5600 has got 300.000xmag and EDS system.
we have one kind of chose for prices. If we study together (I mean we
preapare a paper together), we reseach samples without any price.
Thanks for your interests..
I am waiting for your emails

**********************************
**********************************
** Research.Asst.ERDEM YASAR **
** University of Kirikkale **
** Department of Physics **
** TEM LABORATORY **
** 71450 KIRIKKALE/TURKEY **
** erdem.yasar-at-physics.org **
** yasar-at-science.ankara.edu.tr **
** yasar-at-turkuaz.kku.edu.tr **
**********************************
**********************************

Home Page: http://turkuaz.kku.edu.tr/~yasar







From daemon Mon Nov 27 18:26:19 2000



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Tue, 28 Nov 2000 13:23:47 GMT+1200
Subject: Re: ABOUT JSM5600 SEM AND JEM3010 TEM

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I hate to seem unkind, but isn't once enough for this?

cheers

rtch


} Date: Mon, 27 Nov 2000 19:43:34 +0200 (EET)
} From: Erdem Yasar {yasar-at-turkuaz.kku.edu.tr}
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Re: ABOUT JSM5600 SEM AND JEM3010 TEM

} --------------------------------------------------------------------
} ---- The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------------
} ---.
}
}
} Dear Sir/Madam ,
} JEM3010 was manufactured in 1999.it has got 1.500.000x mag and
} digital
} camera system JSM5600 has got 300.000xmag and EDS system.
} we have one kind of chose for prices. If we study together (I mean
} we preapare a paper together), we reseach samples without any price.
} Thanks for your interests..
} I am waiting for your emails
}
} **********************************
} **********************************
} ** Research.Asst.ERDEM YASAR **
} ** University of Kirikkale **
} ** Department of Physics **
} ** TEM LABORATORY **
} ** 71450 KIRIKKALE/TURKEY **
} ** erdem.yasar-at-physics.org **
} ** yasar-at-science.ankara.edu.tr **
} ** yasar-at-turkuaz.kku.edu.tr **
} **********************************
} **********************************
}
} Home Page: http://turkuaz.kku.edu.tr/~yasar
}
}
}
}
}
Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand



From daemon Mon Nov 27 19:00:27 2000



From: Gordon Vrololjak :      gvrdolja-at-nature.Berkeley.EDU
Date: Mon, 27 Nov 2000 16:55:59 -0800 (PST)
Subject: Balzer's 301 Question

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Hello,
We have a Balzer's 301 Freeze fracture etch unit that worked for some time
with no problems. However, one day the unit was overheating so I changed
the oil in the rotary pump. It worked fine until recently. I found that
one fuse had blown in the Pumping unit control TCS 100. Changing the fuse
did not help, and I noticed the transformer in the pumping unit was
discolored.

I was wondering if anyone has a spare transformer or TCS 100 Pumping unit
control they are not using? If not, can anyone suggest why the
transformer chose to die on us at this moment? Previously we kept the
unit pumping constantly. Due to a vacuum leak, I shut it off until it was
to be used for coating. Unfortunately, now it won't start up.
Gordon.

\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\
Gordon Ante Vrdoljak Electron Microscope Lab
ICQ 23243541 http://nature.berkeley.edu/~gvrdolja 26 Giannini Hall
gvrdolja-at-nature.berkeley.edu UC Berkeley
phone (510) 642-2085 Berkeley CA 94720-3330
fax (510) 643-6207 cell (510) 290-6793




From daemon Mon Nov 27 23:53:37 2000



From: jekstrom-at-mediaone.net () (by way of Nestor J. Zaluzec)
Date: Mon, 27 Nov 2000 23:47:45 -0600
Subject: Ask-A-Microscopist: Apergon objective lens

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Colleagues...

Can anyone answer this question . Remember
Ask-A-Microscopist is a forum that is used by NON-MICROSCOPISTS
looking for help so keep try to your technical details at the right level.

Nestor
---------------------------------------------------------------------------

Email: jekstrom-at-mediaone.net
Name: Jim Ekstrom
School: Phillips Exeter
State: NH

Question: I picked up an objective lens that is made in the US. It has
Apergon (in italics) with 200X below it. The lens which is roughly the
size of a normal 10X objective had a different thread than a normal
objective. Can you tell me anything about it?

---------------------------------------------------------------------------





From daemon Tue Nov 28 02:04:01 2000



From: Henrik Kaker :      Henrik.Kaker-at-guest.arnes.si
Date: Tue, 28 Nov 2000 08:58:58 +0100
Subject: Java EMSA Spectrum Plotting Script

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Dear All,

I'm looking a Java EMSA spectrum plotting script for web browsers.

--
Henrik Kaker
Metal Ravne d.o.o.
SEM-EDS Lab
Koroska cesta 14, 2390 Ravne
Slovenia
Phone: +386 02 82 21 131
Fax: +386 02 82 20 436
http://www.kaker.com





From daemon Tue Nov 28 02:05:59 2000



From: Radostin Danev :      rado-at-nips.ac.jp
Date: Tue, 28 Nov 2000 17:02:04 +0900
Subject: Re: ABOUT JSM5600 SEM AND JEM3010 TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} we have one kind of chose for prices. If we study together (I mean we
} preapare a paper together), we reseach samples without any price.

If you study alone - how much for a paper? (Joking ... sorry couldn't stop
myself)

Rado

---------------------------------------------------------------------
Radostin Danev
Laboratory of Ultrastructure Research
National Institute for Physiological Sciences
Myodaiji-cho, Okazaki 444-8585, JAPAN
e-mail: rado-at-nips.ac.jp
---------------------------------------------------------------------






From daemon Tue Nov 28 03:24:51 2000



From: ken blight :      blight-at-icrf.icnet.uk
Date: Tue, 28 Nov 2000 09:18:45 +0000
Subject: MethylCellulose

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Does anyone have a method for removing hardened methycellulose from
cryocections when the film is too thick?





From daemon Tue Nov 28 04:32:55 2000



From: C.L.Healey :      MBA00CLH-at-sheffield.ac.uk
Date: Tue, 28 Nov 2000 10:27:20 +0000
Subject: optical microscopes

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Hi,

I just wondered whether it would be at all possible to ask you a
question,

"Is the resolution of an optical microscope better with green or blue
light?".

Thanks for your help,

Claire
mba00clh-at-sheffield.ac.uk



From daemon Tue Nov 28 06:59:51 2000



From: Jim at ProSciTech :      jim-at-proscitech.com
Date: Tue, 28 Nov 2000 23:16:29 +1000
Subject: RE: optical microscopes

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Claire
Blue is best
To a rough approximation the resolving power is limited to half the
wavelength of the light used. Thus blue -at-480nm = 240 nm, green
-at- 540nm = 270 nm
Chris

Date sent: Tue, 28 Nov 2000 10:27:20 +0000
} From: "C.L.Healey" {MBA00CLH-at-sheffield.ac.uk}
Organization: The University of Sheffield
To: microscopy-at-sparc5.microscopy.com


If its a good microscope, then under oil immersion the blue would give better
resolution because blue light has a shorter wavelengths than green, but . . .
Some people may find blue harder on the eye and less subjectively, it depends
on the colours of the specimen too. If highest resolution is the object then
the specimen should be very thin, say 2 um or less and at that point contrast
becomes an issue and its useful to know that the filter will render the
complimentary colours within the specimen more darkly.
So you could have fun and draw an Oswald colour circle (rainbow colours
arranged in a circle with complimentary colours opposite) and figure out which
filter would render which colours in the specimen darker or lighter.
Thus specimen contrast could be enhanced and green will be more useful to
optimize contrast with more specimens.
Such fancy colour considerations apply to viewing and B+W photography, whereas
in colour photography most people would prefer realistic colours and that, with
a daylight type film requires the right blue filter and the transformer set to
the bulb's voltage.
There is also an argument to be made that all of that is too complicated and
that a digital camera is more fun.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Tuesday, November 28, 2000 8:27 PM, C.L.Healey
[SMTP:MBA00CLH-at-sheffield.ac.uk] wrote:
}
} Hi,
}
} I just wondered whether it would be at all possible to ask you a
} question,
}
} "Is the resolution of an optical microscope better with green or blue
} light?".
}
} Thanks for your help,
}
} Claire
} mba00clh-at-sheffield.ac.uk




From daemon Tue Nov 28 07:45:35 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Tue, 28 Nov 2000 07:40:59 -0600
Subject: MSA Undergraduate Research Scholarship Program

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Press Release for Fall 2000
Microscopy Society of America
Undergraduate Research Scholarship Program

With this year's call for applications the MSA Undergraduate Research
Scholarship Program begins its 13th year providing funding for
undergraduate research. To date over 65 projects covering a wide
range of topics in the physical and biological sciences have received
support through this program. Over the years nearly all the
scholarship recipients have maintained a strong interest in imaging
sciences and have gone on to graduate school, professional school,
teaching, or industry positions.

The program, which is funded by MSA, is able to support approximately
30% to 40% of applicants. The maximal award from MSA is currently
$3000 and helps to provide student stipends, supply costs, and
limited travel expenses associated with the research. Additional
support in the form of instrument use time, equipment purchases, etc.
is generally provided by the studentís supervisor and/or through the
sponsoring institution. Abstracts reporting the research results,
are prepared by scholarship awardees, and published in "Microscopy
and Microanalysis".

The program actively seeks external sources of matching funds in
order to maintain the favorable levels of support both in terms of
the number of projects supported and the level of support for each.
We are extremely grateful for the matching support provided by MSA
sustaining members and individuals. Their support has enabled the
program to increase both the number of awards and the maximum amount
of each award to $3000.

The MSA Undergraduate Research Scholarship Program is currently
soliciting applications from students interested in conducting a
research project which involves the use of any microscopy technique.
Students should be sponsored by a member of MSA. The maximal award
is $3000.
**The application deadline is Dec 31, 2000.**
Applications can be obtained from the MSA Business Office,
businessoffice-at-msa.microscopy.com or call toll free at (800) 538-3672.
If you have any questions or require additional information regarding
the program please contact either:

Dr. Ralph Albrecht, University of Wisconsin
1656 Linden Drive, Madison, WI 53706
(608) 263-3952 or 263-4162; (608) 262-7420 FAX;
albrecht-at-ahabs.wisc.edu

Dr. Richard Ornberg, Monsanto Company
Analytical Sciences Center, 800 N. Lindbergh Blvd., St. Louis, MO 63167
(314) 694-1184; (314) 694-6727 FAX;
rlornb-at-ccmail.monsanto.com





From daemon Tue Nov 28 07:46:26 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Tue, 28 Nov 2000 07:42:21 -0600
Subject: Materials Scientist/MicroCharacterization Position Opening at DoE

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Colleagues...

A Job position for a Materials Scientists with a background in
electron beam microcharacterization has opened today and has
been posted on the US DoE BES web site:

(Position Number PN-01-SC-13-082, GS-1301-15).

http://www.science.doe.gov/production/bes/BESjobs.html

An appointee will be required to provide verification of U. S.
citizenship and/or employment eligibility under the Immigration
Reform and Control Act of 1986 (Public Law 99-603). If selected,
a male applicant born after December 31, 1959, must confirm his
selective service registration status

Applications will be accepted through January 26, 2001.

Cheers... Nestor
Your Friendly Neighborhood SysOp

P.S. Contact DoE at BES-at-science.doe.gov for additional information





From daemon Tue Nov 28 07:50:23 2000



From: Kromer, Barry :      KROMERB-at-cellutissue.com
Date: Tue, 28 Nov 2000 08:46:21 -0500
Subject: Camera adapter

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I am looking for an adapter to mount an Olympus D340 digital camera to a
standard stereo microscope eyepiece. Does anyone have a possible
solution for my problem?
Thanks.
Barry Kromer
kromerb-at-cellutissue.com




From daemon Tue Nov 28 08:32:19 2000



From: Ni, Chao-Ying :      CYNi-at-rodel.com
Date: Tue, 28 Nov 2000 09:23:18 -0500
Subject: Seeking a H2O recirculator (air cooled)

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Dear colleagues,

We have a JSM-845 SEM with a fairly old water recirculator. Because of the
age, the water recirculator does not work properly and needs to be replaced.
Could anybody help me locate a used chiller for the SEM? The chiller itself
has to be air cooled. Thanks very much.

Chao-Ying Ni
Rodel Inc.
Materials Development Center
451 Bellevue Road
Newark, DE 19713



From daemon Tue Nov 28 09:04:27 2000



From: Ni, Chao-Ying :      CYNi-at-rodel.com
Date: Tue, 28 Nov 2000 09:56:17 -0500
Subject: optical microscopes

Contents Retrieved from Microscopy Listserver Archives
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Yes, you are right. The resolution of a microscope is proportional to the
wave length of the light you use for illumination. The "green" or "blue"
light has shorter wave length compared with other lights/colors, hence has
higher resolving capability, e.g., point-to-point resolution. Also, "white
light" is composed of all of "the lights" in the visible range of the light
spectrum, and it may cause so-called chromatic aberration for microscopes.

-cy
Rodel

-----Original Message-----
} From: C.L.Healey [mailto:MBA00CLH-at-sheffield.ac.uk]
Sent: Tuesday, November 28, 2000 5:27 AM
To: microscopy-at-sparc5.microscopy.com


Hi,

I just wondered whether it would be at all possible to ask you a
question,

"Is the resolution of an optical microscope better with green or blue
light?".

Thanks for your help,

Claire
mba00clh-at-sheffield.ac.uk



From daemon Tue Nov 28 09:51:59 2000



From: Tobias Baskin :      BaskinT-at-missouri.edu
Date: Tue, 28 Nov 2000 09:46:35 -0600
Subject: RE: optical microscopes

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Greetings,
Two other points to keep in mind:

1) Detector sensitivity. As a rule, the human eye is more sensitive
to green than blue. Other detectors (cameras, film, etc) will have
their own wavelength dependence.

2) Sample character. A living cell is more likely to be
hurt/stimulated by blue than green light.

Just a few more tangential remarks on this thread.

Tobias
--
_ ____ __ ____ Tobias I. Baskin
/ \ / / \ / \ \ 109 Tucker Hall
/ / / / \ \ \ Biological Sciences
/_ / __ /__ \ \ \__ University of Missouri
/ / / \ \ \ Columbia, MO USA
/ / / \ \ \ 65211-7400
/ / ___ / \ \__/ \ ____ voice: 573-882-0173
fax: 573-882-0123



From daemon Tue Nov 28 09:52:04 2000



From: William F. Tivol :      wft03-at-health.state.ny.us
Date: Tue, 28 Nov 2000 10:47:10 -0500
Subject: Re: optical microscopes

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I just wondered whether it would be at all possible to ask you a
question,

"Is the resolution of an optical microscope better with green or blue
light?".

Thanks for your help,
Dear Claire,
In theory, the best attainable resolution with blue light would be slightly
better than that with green light; however, in practise, the lenses for a given
microscope might be designed to have lower spherical aberration for green light
(if, for example, the scope were designed to use all wavelengths of visible
light, green would be a better compromise than blue), and the properties of the
specimen would probably play a bigger role in attainable resolution than the
wavelength of light used to observe it, for example, a specimen damaged by the
shorter wavelength light would be better observed using the longer wavelength.
Also, the medium used to observe or record the image might be capable of better
resolution with green light. Now that I've probably told you more than you
wanted to know, I can say that it is always possible to ask a question, as long
as you are willing to put up with the answer.
Yours,

Bill Tivol
Wadsworth Center
Albany NY
(518) 473-7399 WFT02-at-health.state.ny.us





From daemon Tue Nov 28 09:53:07 2000



From: Philip Oshel :      peoshel-at-facstaff.wisc.edu
Date: Tue, 28 Nov 2000 09:52:37 -0600
Subject: MSA student scholarship clarification

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Listmembers:

In response to a question, I would like to add this bit to the MSA
undergraduate scholarship announcement:

This is not limited to students in the USA or North America. Any
undergraduate student anywhere is eligible to apply for the award,
provided the person supervising their research is a member of MSA.

Phil
--
}}}}}}}}}}}}}}}}}{{{{{{{{{{{{{{{{{
Philip Oshel
Supervisor, AMFSC and BBPIC microscopy facilities
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706 - 1284
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)



From daemon Tue Nov 28 10:22:40 2000



From: Dr Malcolm Roberts :      m.roberts-at-ru.ac.za
Date: Tue, 28 Nov 2000 16:16:59 +0200
Subject: Fe-rich samples

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Hallo Folks,
We're fiddling around with some rather Fe-rich amphiboles here.
Analysis by WD on our trusty JEOL 733. Totals are coming out a bit high
for an amphibole, and we've started discussing the problem a bit. One of
us has had a bell rung with regards to ZAF correction on Fe-rich
silicates. Anyone have any more ideas on this, and how we might do a bit
of believable scientific fudging?
Cheers,
Malc.

--
Dr MP Roberts Phone: [+27](0)46 603 8313
Dept of Geology Fax: [+27](0)46 622 9715
Rhodes University Cell: 083 4060 262 (usually off)
6140 Grahamstown e-mail: m.roberts-at-ru.ac.za
SOUTH AFRICA





From daemon Tue Nov 28 12:06:19 2000



From: Frank Thomas :      thomasf-at-AGC.BIO.NS.CA
Date: Tue, 28 Nov 2000 13:57:27 -0400
Subject: Green or blue?

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A little tangential to this thread, but....

We in the micropaleontology business (or formerly in it) have for years
been staining Foraminiferal and Ostracode shells with green food colouring
to enhance their appearance in binocular microscopes. It seems to bring out
the contrast a bit, and the dye tends to settle in, and accentuate,
morphological features which aid in identification. Green seems to work a
bit better than blue, but as Tobias points out, that may have more to do
with the sensitivity of the human eye. A pretty cheap little trick, too -
you wouldn't believe how many foram shells you can dye with one little
bottle.....

Frank Thomas
Geological Survey of Canada (Atlantic)
Bedford Institute of Oceanography
Dartmouth, Nova Scotia
Canada




From daemon Tue Nov 28 12:28:32 2000



From: Sara Miller :      saram-at-duke.edu
Date: Tue, 28 Nov 2000 13:17:35 -0500 (EST)
Subject: Re: MethylCellulose

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It should just dissolve in water or buffer if you soak the slides for a
while. Then recoat with more dilute MeCell if you want.

On Tue, 28 Nov 2000, ken blight wrote:

} Date: Tue, 28 Nov 2000 09:18:45 +0000
} From: ken blight {blight-at-icrf.icnet.uk}
} To: Microscopy-at-sparc5.microscopy.com
} Subject: MethylCellulose
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Does anyone have a method for removing hardened methycellulose from
} cryocections when the film is too thick?
}
}
}
}

Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265




From daemon Tue Nov 28 13:01:19 2000



From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Tue, 28 Nov 2000 08:54:21 -1000 (HST)
Subject: Reset on Reichert Cryocut

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Hello, all-

I am posting this for a colleague-

We have a Reichert-Jung Cryocut 1800 cryostat. The motor which moves the
specimen in and out is stuck in the STOP, fully advanced position and will
not retract. I know there is a manual way to get the specimen holder back
to its Home position, but I have forgotten, and it is not in my
manual. Can anyone help me with this?

Mahalo!

Dr. David T. Webb
University of Hawaii at Manoa
Dept. of Botany
dave-at-hawaii.edu

You may email him directly, or I will forward any replies.

High 70s F, windy, intermittant clouds. Beautiful November light.

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************




From daemon Tue Nov 28 15:15:05 2000



From: Gerroir, Paul J :      Paul.Gerroir-at-crt.xerox.com
Date: Tue, 28 Nov 2000 15:51:20 -0500
Subject: TEM - Availability of Transparent Sleeves for TEM Negatives?

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November 28, 2000

To all,
I just found out that Kodak has discontinued the manufacture of its
transparent sleeves, CAT 150 3812. Does anyone know of a suitable
replacement for this product and where it can be purchased, preferably in
either Canada or the N. Eastern U.S.?

Thanks for your help.
Paul

Paul J. Gerroir
Microscopy
Materials Characterization
Xerox Research Centre of Canada
2660 Speakman Drive
Mississauga, Ontario L5K 2L1

Phone: (905) 823-7091, ext. 216
FAX: (905) 822-7022
email: paul.gerroir-at-crt.xerox.com




From daemon Tue Nov 28 16:07:19 2000



From: Lesley Weston :      lesley-at-interchange.ubc.ca
Date: Tue, 28 Nov 2000 14:00:56 -0800 (PST)
Subject: Re: optical microscopes

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Blue, but to such a small extent that it really can't make much
difference. The shorter the wavelength, the higher the
resolution. However, for some black and white films used for
photomicrography, such as Technical Pan, a green filter gives better
results. Hope this helps.

Lesley Weston.



On Tue, 28 Nov 2000, C.L.Healey wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hi,
}
} I just wondered whether it would be at all possible to ask you a
} question,
}
} "Is the resolution of an optical microscope better with green or blue
} light?".
}
} Thanks for your help,
}
} Claire
} mba00clh-at-sheffield.ac.uk
}
}




From daemon Tue Nov 28 19:43:08 2000



From: richard black :      m02jmy00-at-cwcom.net
Date: Wed, 29 Nov 2000 01:28:52 +0000
Subject: Edwards E306

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Jonathan,
Tried to send message but got undeliverable message. If you want to
contact me direct I could give you some tips if that is any use. I have
used one very regularly for carbon coating for some years, with a good
degree of success.
cheers.
Richard Black




From daemon Tue Nov 28 22:59:52 2000



From: Andrew Jephcoat :      Andrew.Jephcoat-at-earth.ox.ac.uk
Date: Tue, 28 Nov 2000 22:53:40 -0600
Subject: Searching for Leitz Wetzlar LWD Objective

Contents Retrieved from Microscopy Listserver Archives
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To: ListServer
Re: Please post the message below. Thank you.

Would like to purchase (now unobtainable) Leitz Wetzlar
long working distance objective originally
manufactured for a Universal Stage. 1or 2 off.
Manufacturer Part number : 559123
Barrell details: 160/- L25/0.22 (P) (UT40/0.34).

Any information on possible source would be welcome.

--
Dr Andrew P. Jephcoat +44-(0)1865-272067
Department of Earth Sciences
Parks Road +44-(0)1865-272000 (Dept.)
Oxford OX1 3PR +44-(0)1865-272072 (FAX)
UK andrew-at-
earth.ox.ac.uk
http://www.earth.ox.ac.uk/Research/interior.htm





From daemon Wed Nov 29 03:26:46 2000



From: Chris Jeffree :      cjeffree-at-srv0.bio.ed.ac.uk
Date: Wed, 29 Nov 2000 09:17:28 +0000
Subject: Re: optical microscopes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Chuck Berger wrote:
} Take into account that the eye is most sensitive in the green }
} region & what color is your objective corrected for?

Yes, but sensitivity is a detector property, not a property of the
optics.

Correct me if I am wrong, but the corrections made to an
apochromat are primarily to bring different wavelengths to the same
plane of focus, not primarily to optimise for resolution at a particular
wavelength

Lesley Weston wrote:
} Blue, but to such a small extent that it really can't make much
} difference. The shorter the wavelength, the higher the
} resolution. However, for some black and white films used for
} photomicrography, such as Technical Pan, a green filter gives
} better results. Hope this helps.

Again this is a detector-sensitivity issue which does not alter the
fundamental fact that diffraction-limited resolving power of light
optics is higher in blue light than in green.

One of the reasons why users of Panchromatic films prefer to work
with a green filter is that it provides more natural-looking contrast
and grey-scale values, for the very reason that Chuck Berger is
referring to - that the eye has better brightness and contrast
sensitivity in green. Technical Pan has red sensitivity extended into
the near infrared, and contributions to the image from these
wavelengths may certainly degrade resolution of the recorded
image a) because they may not focus at the same plane as green
light, and b) because these longer wavelengths cannot form
diffraction limited image details as small as the shorter blue and
green wavelengths. The relationship between wavelength and
resolution becomes important here - because the near infra red
(~900-1000nm) has twice the wavelength of blue/blue-green (450-
500nm) the diffraction limited resolution is poorer by a factor of two.

Incidentally, such considerations are also important when using
monochrome CCD cameras. Many of these (such as Pulnix TM6,
for example) have spectral sensitivity extended into the infrared
(considerably further than Technical Pan). IR forms a large fraction
of the illumination intensity of a light microscope unless an IR filter
is fitted. The IR component degrades both resolution and contrast,
and the problem is in this case not adequately resolved by using a
green filter alone, since most transmit IR. Use of a heat-absorbing
glass filter, or even a petri dish with a layer of dilute copper
sulphate solution, will improve performance in such systems quite
dramatically.

(PS this will not help improve the performance of your colour ccd
camera, so don't ask :-)

Best wishes
Chris
==========================================
Dr. Chris Jeffree
University of Edinburgh
BIOSEM - Biological Sciences Electron Microscope Facility
Institute of Cell and Molecular Biology
Daniel Rutherford Building
King's Buildings, Mayfield Road
EDINBURGH, EH9 3JH, Scotland, UK
Tel. #44 (0) 131 650 5345
FAX. #44 (0) 131 650 6563
Mobile 07710 585 401
email c.jeffree-at-ed.ac.uk
=========================================



From daemon Wed Nov 29 08:41:26 2000



From: Blancaflor, Elison :      eblancaflor-at-noble.org
Date: Wed, 29 Nov 2000 08:32:40 -0600
Subject: RA position available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Research Assistant
Samuel Roberts Noble Foundation
A research assistant position is available to join the microscopy support
group. The research assistant will be assigned to work on various projects
using microscopy techniques. This will involve immunocytochemistry (light
and electron), in situ hybridization and confocal microscopy. A B.S. degree
in Biology and previous experience in light and electron microscopy of plant
material is required. Organizational skills and the ability to work
collaboratively with various research groups are essential. Salary is
commensurate with experience ($28,000 to $39,200.00). Application and job
description obtainable from the Noble Foundation website, www.noble.org
{../index.html} . To apply, send a letter outlining research interests and
experience, CV and names of 3 references to:

Jane Nance, Human Resources Department
RE: Position #PltBio30700-EB147
Samuel Roberts Noble Foundation
PO Box 2180
Ardmore, OK 73402, USA.



From daemon Wed Nov 29 08:45:48 2000



From: Philip Oshel :      peoshel-at-facstaff.wisc.edu
Date: Wed, 29 Nov 2000 08:43:52 -0600
Subject: Re: optical microscopes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Chris,

You're not wrong, but there is a subtle point here: the optics are
designed around green light, typically 550nm, and then corrected for
blue and red (if apochromatic). This means the the optics work
optimally in green light, and therefore would resolve green light
best. Theoretically, shorter, blue light has higher resolution, but
the theory isn't taken advantage of unless the optics are designed
for blue light. Note that the filter in phase contrast microscopes is
typically green, because the phase annulus in the objectives is
designed for 550nm, because the (uncorrected) optics are designed for
550nm. +/- of course.

Phil

} Chuck Berger wrote:
} } Take into account that the eye is most sensitive in the green }
} } region & what color is your objective corrected for?
}
} Yes, but sensitivity is a detector property, not a property of the
} optics.
}
} Correct me if I am wrong, but the corrections made to an
} apochromat are primarily to bring different wavelengths to the same
} plane of focus, not primarily to optimise for resolution at a particular
} wavelength
--
}}}}}}}}}}}}}}}}}{{{{{{{{{{{{{{{{{
Philip Oshel
Supervisor, AMFSC and BBPIC microscopy facilities
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706 - 1284
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)



From daemon Wed Nov 29 09:14:06 2000



From: Matthew Lynn :      mlynn-at-miami.edu
Date: Wed, 29 Nov 2000 11:11:13 -0500
Subject: RE: TEM - Availability of Transparent Sleeves for TEM Negatives?

Contents Retrieved from Microscopy Listserver Archives
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Robert
No need to apologize! On the contrary, many thanks for your most
informative comments. You clearly know rather more about it than I
do!
Best wishes
Chris

Date sent: Wed, 29 Nov 2000 09:25:16 -0500 (EST)
} From: Robert Wieland {wieland-at-ME.UDel.Edu}
To: c.jeffree-at-ed.ac.uk



Paul,

I don't know the size or cost of those Kodak sleeves, but I have used Print
File archival sleeves from Get Smart Products in Manhasset, NY. According to
my 1999 catalog:

Phone: (800) 827-0673
Fax: (516) 365-0673

Hope this helps!
Matt

Matthew J. Lynn, Ph.D.
Center for Advanced Microscopy
University of Miami
(305)284-4736
mlynn-at-miami.edu

On Tuesday, November 28, 2000 3:51 PM, Gerroir, Paul J
[SMTP:Paul.Gerroir-at-crt.xerox.com] wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} November 28, 2000
}
} To all,
} I just found out that Kodak has discontinued the manufacture of its
} transparent sleeves, CAT 150 3812. Does anyone know of a suitable
} replacement for this product and where it can be purchased, preferably in
} either Canada or the N. Eastern U.S.?
}
} Thanks for your help.
} Paul
}
} Paul J. Gerroir
} Microscopy
} Materials Characterization
} Xerox Research Centre of Canada
} 2660 Speakman Drive
} Mississauga, Ontario L5K 2L1
}
} Phone: (905) 823-7091, ext. 216
} FAX: (905) 822-7022
} email: paul.gerroir-at-crt.xerox.com




From daemon Wed Nov 29 13:30:09 2000



From: Jesse Rodrigues :      Jesse_Rodrigues-at-kopin.com
Date: Wed, 29 Nov 2000 14:22:44 -0500
Subject: Imaging etched quartz

Contents Retrieved from Microscopy Listserver Archives
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Hi,

Does anyone have any experience imaging etched quartz or glass in a
FESEM?

Thank you,
Jesse

Jesse Rodrigues
Kopin Corporation
695 Myles Standish Blvd
Taunton, MA 02780
jrodrigues-at-kopin.com
(508) 824-6696





From daemon Wed Nov 29 14:04:31 2000



From: jmkrupp-at-cats.ucsc.edu (Jon Krupp)
Date: Wed, 29 Nov 2000 11:57:22 -0800
Subject: ultramicrotome room?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greetings:

Do you have any advice regarding environmental controls for a small room to
be used for ultrathin sectioning?

I have been asked for recommendations regarding things like air filters,
temperature control, and humidity.

It will be a small room, maybe 6' x 9', in a modern university science
building. The researcher is going to put a new ultramicrotome in there and
will want to do serial sectioning on conventional resin blocks. Cryo may
come later, so if there is anything special needed for that, now is the
time to add it to the list.

I have always been OK with standard sort of rooms, regular building air
filters etc, if there is anything special I have overlooked, please let me
know so I can pass it along.

Thanks

Jonathan Krupp
Microscopy & Imaging Lab
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu





From daemon Wed Nov 29 16:15:48 2000



From: Schumacher, Elaine :      efschuma-at-uop.com
Date: Wed, 29 Nov 2000 15:58:42 -0600
Subject: RE: TEM - Availability of Transparent Sleeves for TEM Negatives?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I put in a call to the photographic equipment supplier in the Chicago area
through whom I've ordered the sleeves in the past. He contacted Kodak, and
was told that Kodak is no longer carrying this item (apparently it's not
actually manufactured by Kodak?), but that the sleeves are available from
Tiffen, a maker of filters and other photographic equipment. I was told
that the next time I place an order, I just need to let the purchasing agent
know that the supplier is Tiffen, rather than Kodak. I haven't yet been
able to get contact information for Tiffen (they have a website, but I'm
blocked from accessing it at work), or to verify this by placing an order,
but I thought I'd pass along what I've learned so far. If I find that this
is a dead end, I'll let you know.

Elaine Schumacher
UOP
Des Plaines, IL
847-391-3403
efschuma-at-uop.com



From daemon Wed Nov 29 20:13:05 2000



From: Schumacher, Elaine :      efschuma-at-uop.com
Date: Wed, 29 Nov 2000 15:58:42 -0600
Subject: RE: TEM - Availability of Transparent Sleeves for TEM Negatives?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


I put in a call to the photographic equipment supplier in the Chicago area
through whom I've ordered the sleeves in the past. He contacted Kodak, and
was told that Kodak is no longer carrying this item (apparently it's not
actually manufactured by Kodak?), but that the sleeves are available from
Tiffen, a maker of filters and other photographic equipment. I was told
that the next time I place an order, I just need to let the purchasing agent
know that the supplier is Tiffen, rather than Kodak. I haven't yet been
able to get contact information for Tiffen (they have a website, but I'm
blocked from accessing it at work), or to verify this by placing an order,
but I thought I'd pass along what I've learned so far. If I find that this
is a dead end, I'll let you know.

Elaine Schumacher
UOP
Des Plaines, IL
847-391-3403
efschuma-at-uop.com





From daemon Wed Nov 29 20:50:28 2000



From: Schumacher, Elaine :      efschuma-at-uop.com
Date: Wed, 29 Nov 2000 15:58:42 -0600
Subject: RE: TEM - Availability of Transparent Sleeves for TEM Negatives?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


I put in a call to the photographic equipment supplier in the Chicago area
through whom I've ordered the sleeves in the past. He contacted Kodak, and
was told that Kodak is no longer carrying this item (apparently it's not
actually manufactured by Kodak?), but that the sleeves are available from
Tiffen, a maker of filters and other photographic equipment. I was told
that the next time I place an order, I just need to let the purchasing agent
know that the supplier is Tiffen, rather than Kodak. I haven't yet been
able to get contact information for Tiffen (they have a website, but I'm
blocked from accessing it at work), or to verify this by placing an order,
but I thought I'd pass along what I've learned so far. If I find that this
is a dead end, I'll let you know.

Elaine Schumacher
UOP
Des Plaines, IL
847-391-3403
efschuma-at-uop.com





From daemon Thu Nov 30 00:14:34 2000



From: Sampath, Srinidhi (CORP, GEITC) :      Srinidhi.Sampath-at-geind.ge.com
Date: Thu, 30 Nov 2000 09:14:59 +0530
Subject: LM: Views on Spot cameras

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} Hi everyone:
} I would like the society's views on the Spot RT-SLider digital camera. How
} long does it take to capture an image (fluorescence) and any hardware /
} software bugs experienced by users?
}
} S. Srinidhi,
} Materials Scientist
} gJohn F. Welch Technology Center
} _______________________________________
} Inorganic Materials Laboratory
} GEITC, Upper ground floor. Unit IV,
} Innovator building, International Tech Park,
} Whitefield Road, Bangalore - 560066.
} Phone: 8410702 / 03 / 08 / 09 / 841 1580 x: 1176
} Fax:8410704.
} e-mail: srinidhi.sampath-at-geind.ge.com
}
}



From daemon Thu Nov 30 02:57:59 2000



From: E.M.M. Manders :      e.manders-at-chem.uva.nl
Date: Thu, 30 Nov 2000 09:46:15 +0100
Subject: Focus on Microscopy 2001 in Amsterdam

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************************************************
Plan Now to Participate FOCUS ON MICROSCOPY 2001
************************************************

Focus on Microscopy 2001 (FOM 20001) is a world leading international
conference on advanced microscopy. It is the joint meeting of the 13th
International Conference on Confocal Microscopy and the 13th International
Conference on 3D Image Processing in Microscopy.

FOM 2001 will be held on April 1-4, 2001 at the Academic Medical Centre
(AMC) of the University of Amsterdam, Amsterdam, The Netherlands.


For further information: http://www.focusonmicroscopy.org/
----------------------------------------------------------



Important Dates:
----------------
Camera-ready Abstract Due Date: February 1, 2001
Early Registration Due Date: February 15, 2001
Hotel Reservation Due Date: February 15, 2001


Location:
---------
The Academic Medical Centre (AMC) of the University of Amsterdam is
situated only 15 minutes from the Amsterdam city centre where you will find
all sorts of restaurants, museums, concert halls historical buildings and
nice pubs. The organisation provides you with a public transport card, so
you can easily find your way from your hotel, through the city centre, to
the conference.


Hotel Accommodation:
--------------------
Hotels can be reserved through the RAI Hotel Service. See hotelform on the
website (or ask at the Conference Office). The Conference Office does not
make reservations. You can also make you own (cheaper) reservation through
on-line hotel reservation sites such as http://www.medialink.nl/amsterdam .


Conference Topics:
------------------
Theory, Instrumentation, 4D-Imaging of living cells, Multi-photon
excitation, Recovery after photobleaching (FRAP, FLIP), Fluorescence energy
transfer (FRET), Non-linear optics (CARS, SHG, THG), Time-resolved imaging
(FLIM), Green fluorescent protein (GFP), 4D- and 3D-Image processing,
reconstruction and analysis, Virtual Reality (VR), X-Ray microscopy, Near
field microscopy, Industrial applications in lithography and data storage,
and many other techniques and applications.

This year, there will be special attention for the issue: "Microscopy of
living cells and tissue"


International Organizing Commitee:
----------------------------------
G.J. Brakenhoff, University of Amsterdam, The Netherlands; A.C. Boccara,
ESPCI, France; P.C. Cheng, SUNY at Buffalo, USA; C. Cogswell, University of
Sydney, Australia; J. Dobrucki, Jagiellonian University, Poland; R. Van
Driel, University of Amsterdam, The Netherlands; M. Gu, Swinburne
University of Technology, Australia; K.S. Ha, Korea Basic Science
Institute, Korea; S.W. Hell, MPI for Biophysical Chemistry, Germany; B.
Hermann, University of Texas HSC at San Antonio, USA; F.J. Kao, National
Sun Yat-Sen University, Taiwan; S. Kawata, Osaka University, Japan; A.
Kriette, University of Giessen, Germany; E.M.M. Manders, University of
Amsterdam, The Netherlands; M. Müller, University of Amsterdam, The
Netherlands; C. Saloma, University of Philippines, Philippines; C.J.R.
Sheppard, University of Sydney, Australia; E.H.K. Stelzer, EMBL, Germany;
T. Wilson, University of Oxford, UK.


Conference Office:
------------------
Mrs M.P.A. Beunk-Timmers
Nicolaes Tulp Institute
PO-Box 23213
1100 DS Amsterdam
The Netherlands
Fax: +31-(0)20-6963228
Phone +31-(0)20-5668585
Web: http://www.FocusOnMicroscopy.org
E-mail: info-at-FocusOnMicroscopy.org


Local Organising Committee:
---------------------------
Prof. G.J. Brakenhoff and Dr. E.M.M. Manders




---------------
Erik M.M. Manders, PhD
Swammerdam Institute for Life Sciences
Faculty of Science, University of Amsterdam

Visit: Kruislaan 316, Biulding III, room 2.07, Amsterdam, The Netherlands
Plantage Muidergracht 12, 4th floor, Amsterdam, The Netherlands
Mail: Kruislaan 316
1098 SM AMSTERDAM
The Netherlands
E-mail: e.manders-at-chem.uva.nl
Tel: +31-(0)20-5256225 (5257702)(5255136)
Fax: +31-(0)20-5256271
Web: http://wwwmc.bio.uva.nl/
http://www.FocusOnMicroscopy.org/
---------------




From daemon Thu Nov 30 03:34:50 2000



From: Malcolm Haswell :      malcolm.haswell-at-sunderland.ac.uk
Date: Thu, 30 Nov 2000 09:19:57 +0000
Subject: Re: ultramicrotome room?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


John

if cryo will be used later, it might be worth considering whether there will be
enough ventilation to prevent a build up of nitrogen gas and subsequent oxygen
depletion. If nothing else is done then an oxygen depletion monitor should be
top of the list before liquid nitrogen is used or stored in such a small room.

The sums for calculating oxygen loss when liquid nitrogen is evaporated are
fairly straight forward and I would always assume the worst scenario.

In the last two years there have been two fatal incidents, that I know of,
which have involved oxygen depletion so even if the use of liquid nitrogen is
in the future and a remote possibility it's worth considering now.

On a more trivial note it might be worth specifying floor and benches which
don't crack with constant liquid nitrogen spillages.

I hope this helps.

Malcolm Haswell
e.m. unit
School of Sciences
University of Sunderland
UK

Jon Krupp wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Greetings:
}
} Do you have any advice regarding environmental controls for a small room to
} be used for ultrathin sectioning?
}
} I have been asked for recommendations regarding things like air filters,
} temperature control, and humidity.
}
} It will be a small room, maybe 6' x 9', in a modern university science
} building. The researcher is going to put a new ultramicrotome in there and
} will want to do serial sectioning on conventional resin blocks. Cryo may
} come later, so if there is anything special needed for that, now is the
} time to add it to the list.
}
} I have always been OK with standard sort of rooms, regular building air
} filters etc, if there is anything special I have overlooked, please let me
} know so I can pass it along.
}
} Thanks
}
} Jonathan Krupp
} Microscopy & Imaging Lab
} University of California
} Santa Cruz, CA 95064
} (831) 459-2477
} jmkrupp-at-cats.ucsc.edu




From daemon Thu Nov 30 06:52:48 2000



From: Roger Moretz :      rcmoretz-at-excite.com
Date: Thu, 30 Nov 2000 04:41:37 -0800 (PST)
Subject: Re: ultramicrotome room?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Jon:
As you have said, the standard conditions and filters are really all that
are necessary. The only thing I have really had a problem with are air
currents. I generally ended up setting up a cublicle type area just barely
large enough for the microtome and me, with a curtain (the "plastic strip"
type used in cold rooms works)to minimize air currents. One other thing
that needs to be monitored/controlled is humidity (more than temperature,
even). Too dry or too humid, and forget sectioning! Of course, I was doing
massive serial sectioning, so controlling the environment became an
obsession. Hope this helps.

Roger Moretz, Ph.D.
Dept of Toxicology
Boehringer Ingelheim Pharmaceuticals

On Wed, 29 Nov 2000 11:57:22 -0800, Jon Krupp wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Greetings:
}
} Do you have any advice regarding environmental controls for a small room
to
} be used for ultrathin sectioning?
}
} I have been asked for recommendations regarding things like air filters,
} temperature control, and humidity.
}
} It will be a small room, maybe 6' x 9', in a modern university science
} building. The researcher is going to put a new ultramicrotome in there
and
} will want to do serial sectioning on conventional resin blocks. Cryo may
} come later, so if there is anything special needed for that, now is the
} time to add it to the list.
}
} I have always been OK with standard sort of rooms, regular building air
} filters etc, if there is anything special I have overlooked, please let
me
} know so I can pass it along.
}
} Thanks
}
} Jonathan Krupp
} Microscopy & Imaging Lab
} University of California
} Santa Cruz, CA 95064
} (831) 459-2477
} jmkrupp-at-cats.ucsc.edu
}
}
}





_______________________________________________________
Tired of slow Internet? Get -at-Home Broadband Internet
http://www.home.com/xinbox/signup.html




From daemon Thu Nov 30 07:38:04 2000



From: Debby Sherman :      dsherman-at-purdue.edu
Date: Wednesday, November 29, 2000 2:57 PM
Subject: Fwd: ultramicrotome room?

Contents Retrieved from Microscopy Listserver Archives
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------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Jonathan,
Assuming the building is completed, the simplist modification to a "typical" room is to install a drip ventilation ceiling rather than one with a normal type of air vent. The drip ceilings are made up of ceiling tiles that have many small slits throughout which allow air to filter down into the room. This type of ventilation virtually eliminates drafts which, aside from vibration, can be a major problem in microtomy.
If you are lucky enough to be designing a room in a building under construction, the ideal situation for microtome and microscope rooms is a "floating " foundation. This is when the room floor (obviously in the lowest level) is on concrete supports that are isolated from the main building foundation. That way vibrations from the building as a whole are not transfered directly to the floor of the room in question. Going along with this is the use of flexible morter where internal walls join outside walls.

Debby


Debby Sherman Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
1057 Whistler Building
West Lafayette, IN 47907-1057
--------------------------------------


Greetings:

Do you have any advice regarding environmental controls for a small room to
be used for ultrathin sectioning?

I have been asked for recommendations regarding things like air filters,
temperature control, and humidity.

It will be a small room, maybe 6' x 9', in a modern university science
building. The researcher is going to put a new ultramicrotome in there and
will want to do serial sectioning on conventional resin blocks. Cryo may
come later, so if there is anything special needed for that, now is the
time to add it to the list.

I have always been OK with standard sort of rooms, regular building air
filters etc, if there is anything special I have overlooked, please let me
know so I can pass it along.

Thanks

Jonathan Krupp
Microscopy & Imaging Lab
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-cats.ucsc.edu







From daemon Thu Nov 30 09:59:48 2000



From: FRIEDA CHRISTIE :      F.Christie-at-rbge.org.uk
Date: Thu, 30 Nov 2000 11:23:20 BST
Subject: Ultramicrotome room

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Jon,

I arrived in post to find that a brand new Ultracut S had been
installed in a converted cleaners cupboard measuring (4' x 10')
located several metres from the building's main plant room.
Despite standing on an anti-vibration table the resonance through
the walls from the plant room mean that I have to time my
sectioning work so that it doesn't coincide with the machinery next
door. In addition, there was a cool air ventilation shaft directly
above the Ultracut that made the water in the knife boat resemble
something out of 'A Perfect Storm'!!!. ( I subsequently had that
blocked off). One other problem that you may encounter in such a
small room is the build up of static electricity due to the dry air. I
find that it helps the fill the sink with water when cutting.
I also suggest that you consider very carefully if it is wise to work
with cryo in such a small area as, I believe that this could be a
major hazard.

These are my personal experiences and I do not speak on behalf of
my organisation, but I hope that they are of some use to you in this
matter.





From daemon Thu Nov 30 12:15:29 2000



From: Xinran Liu :      xinran.liu-at-UTSouthwestern.edu
Date: Thu, 30 Nov 2000 10:29:43 -0600
Subject: Follow-up on Hippocampal EM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


First of all, I would like to thank people who responded to my previous post
regarding problems on cultured hippocampal EM. Special thanks to JoAnn
Buchanan, Dorothy Sorenson and Michael Plociniak and others who provided
valuable suggestion that lead to the fantastic results.

Here are the major modification on my previous protocol:

1. Make sure the culture is healthy.
2. Shorten fixation and dehydration time.
3. Prolong the uranyl acetate staining to 30-40 min.
4. Section at 50-60 nm thickness (setting).
5. Observe cells at 60 kV rather than a higher accelerating voltage.

Hope this helps with people who have the similar problems as I had before.

Xinran

********************************************
Xinran Liu, M.D., Ph.D.
Center for Basic Neuroscience
The University of Texas Southwestern Medical Center at Dallas
6000 Harry Hines Blvd., NA4.214A
Dallas, TX 75390-9111
Phone: (214) 648-1830
Fax: (214) 648-1801
E-mail: xinran.liu-at-utsouthwestern.edu




From daemon Thu Nov 30 12:53:33 2000



From: giselle melville :      giselledgt-at-excite.com
Date: Thu, 30 Nov 2000 10:47:47 -0800 (PST)
Subject: Thesis question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Good afternoon

My name is Giselle. I am a graduate student working on my thesis. My
thesis topic is "Methods of preparation of petrographic thin sections for
Electron Microscopy". I have a collection of thin sections (chert) and I am
preparing the sections for analysis by a TEM 1200. The thin sections are
initially prepared by methods Ultramicrotomy and Ion Milling. TEM
parameters used will be electron diffraction and elemental composition to
obtain identification of the mineral and microstructures. I need more
information (references) on recent literature (1998-2000) on my subject or
similiar work done using these methods for analyis by TEM.

I need your help!!!

Thank you for your time.





_______________________________________________________
Tired of slow Internet? Get -at-Home Broadband Internet
http://www.home.com/xinbox/signup.html




From daemon Thu Nov 30 15:35:59 2000



From: Ladd Research :      sales-at-laddresearch.com
Date: Thu, 30 Nov 2000 16:26:24 -0500
Subject: Looking for address of Company

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Could anyone provide me with a phone number, e-mail or fax number for
the company "RAITH USA" It is a Germany Company and I thought there was
a distrbutor in the US

Thanks

D. Sicard
--

LADD RESEARCH
131 Dorset Lane
Williston, VT 05495 USA

web site http://www.laddresearch.com

TEL 1-800-451-3406 (US) or 1-802-878-6711 (anywhere)
FAX 1-802-878-8074
e-mail sales-at-laddresearch.com

Quality Since 1955



From daemon Thu Nov 30 17:13:44 2000



From: Louise_Harner-at-albint.com
Date: Thu, 30 Nov 2000 18:02:28 -0500
Subject: Pricing suggestions? B&L projection 'scope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


My boss wants to sell one of our projection microscope systems to someone she
knows. I have been given the job of suggesting a price and would appreciate
suggestions from anyone on the listserver. To prevent clutter on the listserver,
please respond to my e-mail address: Louise_Harner-at-albint.com

The projection microscope system has Bausch & Lomb optics. It is basically an
inverted monocular microscope and projects an image onto the tabletop -
magnification of 500X at the table. The top of the lamp house is about 42 inches
above the table. The light goes through a condenser, then through the slide,
into a 20X objective, and out the monocular eyepiece. The normal focusing and
x-y stage controls are supplemented by hand control extensions (x, y, and z) and
by foot control extensions (x and y). There is a catalog number on the lamp
housing: B&L cat. # 31-32-61, but I'm not sure if that is for the lamp only or
the whole system. The system was used primarily to measure fiber diameters.

Thanks in advance for your help!


Louise Harner
Albany International Research Co.
Mansfield, MA

Louise_Harner-at-albint.com




From daemon Thu Nov 30 18:24:40 2000



From: DENISE MARTIN :      DENISE.MARTIN-at-cfisd.net
Date: Thu, 30 Nov 2000 18:16:31 -0600
Subject: Seeking TEM specimens for high school students

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I work for a public school system in Houston, TX. We acquired a Philips
TEM as a donation. It is now installed. We are not set up to prepare
specimens and won't be (if ever) at least until next year.
}
} Am in serach of prepared grids you may not need anymore for our high
} school students to look at. Descriptions of grid would be beneficial,
} i.e. what specimen is and what might be occurring.
}
} Seeking specifically:
} plant tissue (leaves, stems, & roots)
} animal cells
} prokaryotic cells
} samples from various kingdoms
} dividing cells
} human tissue
}
} Please advise if you can help! THANKS!
}
} Denise Martin
} Cy-Fair ISD - Science Resource Center
} 11206 Telge Rd.
} Cypress, TX 77429
} 281-897-4695
}





From daemon Thu Nov 30 21:57:33 2000



From: Jim at ProSciTech :      jim-at-proscitech.com
Date: Fri, 1 Dec 2000 13:18:38 +1000
Subject: RE: ultramicrotome room? Draft elimination

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


In my experience, in most climatic zones a room for ultramicrotomy should be
air-conditioned. This frequently creates drafts and these must be eliminated.
The frequently used louvered airduct ceiling outlets are unsuitable and if they
are blocked this would unbalances a complex AC systems.
The cheapest, highly effective way to make such an outlet draft-free is to
mount a 600mm sheet metal inverted "Chinese hat" below the outlet so that a
150mm gap is maintained.
This hat will break the draft and direct the airflow along the ceiling and then
down the walls. Effectively the outlet area is greatly increased and the air
speed slowed and re-directed.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Thursday, November 30, 2000 10:42 PM, Roger Moretz [SMTP:rcmoretz-at-excite.
com] wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Jon:
} As you have said, the standard conditions and filters are really all that
} are necessary. The only thing I have really had a problem with are air
} currents. I generally ended up setting up a cublicle type area just barely
} large enough for the microtome and me, with a curtain (the "plastic strip"
} type used in cold rooms works)to minimize air currents. One other thing
} that needs to be monitored/controlled is humidity (more than temperature,
} even). Too dry or too humid, and forget sectioning! Of course, I was doing
} massive serial sectioning, so controlling the environment became an
} obsession. Hope this helps.
}
} Roger Moretz, Ph.D.
} Dept of Toxicology
} Boehringer Ingelheim Pharmaceuticals
}
} On Wed, 29 Nov 2000 11:57:22 -0800, Jon Krupp wrote:
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Greetings:
} }
} } Do you have any advice regarding environmental controls for a small room
} to
} } be used for ultrathin sectioning?
} }
} } I have been asked for recommendations regarding things like air filters,
} } temperature control, and humidity.
} }
} } It will be a small room, maybe 6' x 9', in a modern university science
} } building. The researcher is going to put a new ultramicrotome in there
} and
} } will want to do serial sectioning on conventional resin blocks. Cryo may
} } come later, so if there is anything special needed for that, now is the
} } time to add it to the list.
} }
} } I have always been OK with standard sort of rooms, regular building air
} } filters etc, if there is anything special I have overlooked, please let
} me
} } know so I can pass it along.
} }
} } Thanks
} }
} } Jonathan Krupp
} } Microscopy & Imaging Lab
} } University of California
} } Santa Cruz, CA 95064
} } (831) 459-2477
} } jmkrupp-at-cats.ucsc.edu
} }
} }
} }
}
}
}
}
}
} _______________________________________________________
} Tired of slow Internet? Get -at-Home Broadband Internet
} http://www.home.com/xinbox/signup.html
}




From daemon Sun Dec 10 18:24:19 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Sun, 10 Dec 2000 18:21:47 -0600
Subject: Test at 6:21 to TestList

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html







From daemon Fri Dec 1 01:47:58 2000



From: Faerber Jacques :      Jacques.Faerber-at-ipcms.u-strasbg.fr
Date: Fri, 1 Dec 2000 08:41:20 +0100
Subject: To D.Sicard : Raith adress

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



To D. Sicard

Here are two adresses of Raith GmbH, in the USA and in Germany :
Raith INC.
6 Beech Road
Islip
NY 11751-4907
Phone (516)224-1764
Fax (516)224-2620
E-mail 73164.1330-at-compuserve.com

Headquarter in Germany

Raith GmbH
Hauert 18
D-44227 Dortmund
Phone +49 (0)231 975000-0
Fax (0)231 975000-5
E-mail Raith-at-Raith.de

Web site http//WWW.raith.de

I hope the american adress is still right. The german one is good, and you
should be able to verify on the web site.

Bye

J. Faerber
IPCMS-GSI
23, rue de Loess
67037 Strasbourg CEDEX
France

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)0 88 10 72 48
E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr




From daemon Fri Dec 1 03:27:10 2000



From: loy96-at-eeng.dcu.ie (Tom)
Date: Fri, 1 Dec 2000 03:23:17 -0600 (CST)
Subject: Know Your Rights

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


INTERNATIONAL DRIVER'S LICENSE

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or revoked?

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The United Nations gave you the privilege to
drive freely throughout the world! (Convention
on International Road Traffic of September 19,
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Netherlands, January 21, 1958)

Take advantage of your rights. Order a valid
International Driver's License that can never
be suspended or revoked.

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=================================
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=================================

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cuk&#OK - mail from {loy96-at-eeng.dc



From daemon Fri Dec 1 04:30:07 2000



From: Faerber Jacques :      Jacques.Faerber-at-ipcms.u-strasbg.fr
Date: Fri, 1 Dec 2000 11:25:57 +0100
Subject: Hitachi S4700 and Jeol 6700F

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear collègues

We have tested these two FE-SEM and we want to have other opinions about
the differences between these two apparatus. Did you see a big difference
in resolution at low energy (1-2 keV). What about the practical use of the
"In Lens" detector of the S4700 ? Does it really bring more information
than the others ? I think there are some 6700F in Far East (and in the
States ?), but no one in Europe. We are interested in all you can say
about these two SEMs.

Sincerely Yours

J. Faerber
IPCMS-GSI
23, rue de Loess
67037 Strasbourg CEDEX
FRANCE

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)0 88 10 72 48
E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr




From daemon Fri Dec 1 05:28:51 2000



From: Chris Jeffree :      cjeffree-at-srv0.bio.ed.ac.uk
Date: Fri, 1 Dec 2000 11:24:34 +0000
Subject: Re: Hitachi S4700 and Jeol 6700F

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Jacques
We have also evaluated these two microscopes, and I would be interested to
compare notes with you, but not online.
Chris

}
} Dear collègues
}
} We have tested these two FE-SEM and we want to have other opinions about
} the differences between these two apparatus. Did you see a big difference
} in resolution at low energy (1-2 keV). What about the practical use of the
} "In Lens" detector of the S4700 ? Does it really bring more information
} than the others ? I think there are some 6700F in Far East (and in the
} States ?), but no one in Europe. We are interested in all you can say
} about these two SEMs.
}
} Sincerely Yours
}
} J. Faerber
} IPCMS-GSI
} 23, rue de Loess
} 67037 Strasbourg CEDEX
} FRANCE
}
} Tel 00 33(0)3 88 10 71 01
} Fax 00 33(0)0 88 10 72 48
} E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr
}
}


==========================================
Dr. Chris Jeffree
University of Edinburgh
BIOSEM - Biological Sciences Electron Microscope Facility
Institute of Cell and Molecular Biology
Daniel Rutherford Building
King's Buildings, Mayfield Road
EDINBURGH, EH9 3JH, Scotland, UK
Tel. #44 (0) 131 650 5345
FAX. #44 (0) 131 650 6563
Mobile 07710 585 401
email c.jeffree-at-ed.ac.uk
=========================================



From daemon Fri Dec 1 05:43:33 2000



From: AL :      one_2_one_sims-at-yahoo.com
Date: Fri, 1 Dec 2000 05:40:40 -0600 (CST)
Subject: Hi there

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


please check out our latest offers below on pay as you go simcards
121 sims with £10 credit (£3 card and £7 on registration) £8
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From daemon Fri Dec 1 07:28:19 2000



From: Gerroir, Paul J :      Paul.Gerroir-at-crt.xerox.com
Date: Fri, 1 Dec 2000 08:12:17 -0500
Subject: TEM - Availability of TEM Negative Sleeves/sheets

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


December 1, 2000

Thanks to everyone who responded to my cry for help.

To summarize my findings and your suggestions/comments;
Kodak has discontinued the sale of transparent sleeves, CAT 150 3812,
however, that item with the same catalogue number is now being sold by
Tiffen (1-800-368-6257). Of course these sleeves may also be available from
other outlets, eg. National Graphic Supply (1-800-223-7130). A number of
microscopists are using and have suggested I try the polyview sheets which
hold 6 TEM negatives and fit nicely into a 3-ring binder. These sheets, cat.
#1310/3406 are sold by Negafile (1-888-881-6435) but again may also be
available from other outlets, eg. Ted Pella (916-243-2200), in Canada
(1-800-243-7765).

Regards,
Paul

Paul J. Gerroir
Microscopy
Materials Characterization
Xerox Research Centre of Canada
2660 Speakman Drive
Mississauga, Ontario L5K 2L1

Phone: (905) 823-7091, ext. 216
FAX: (905) 822-7022
email: paul.gerroir-at-crt.xerox.com




From daemon Fri Dec 1 09:21:56 2000



From: simon watkins :      swatkins+-at-pitt.edu
Date: Fri, 01 Dec 2000 10:06:14 -0500
Subject: Position available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Senior technician/manager for Electron Microscopy Laboratory
Managerial position for state-of-the-art electron microscopy facility within
the Center for Biologic Imaging, University of Pittsburgh Medical School.
Duties include overseeing and conducting the processing and preparation of
samples for Transmission and Scanning Electron Microscopy and specialized
techniques including cryosectioning and immunoelectron microscopy.
Incumbent will help with training and assisting students and faculty on
equipment, oversee general lab procedures including budget, supply, purchase
orders, photography and quality control. Prior experience in electron
microscopy essential. Bachelor’s or Master’s degree or equivalent desired.
The successful applicant will be responsible for directing the 3 other staff
in the EM component of the center
The Center is a nationally recognized imaging resource using and developing
a complete array of imaging technologies using both light and electron
optics. More details about the center can be found at our web site
(http://sbic6.sbic.pitt.edu):. This position is within the EM component of
the CEnter. We have a full array of prep equipment from freeze fracture to
ultracryomicrotomes. Our microscope base includes two transmission and two
SEM scopes including a new field emission gun SEM.
If you would like further information please contact Dr. Donna Beer Stolz or
myself. Please send applications electronically to dstolz+-at-pitt.edu

---------------------------
Simon C. Watkins Ph.D. MRC Path
Associate Professor, Cell Biology and Physiology
Director: Center for Biologic Imaging
BSTS 225
University of Pittsburgh
Pittsburgh PA 15261
Tel:412-648-3051
Fax:412-648-8330

-----------------------------------




From daemon Fri Dec 1 10:57:15 2000



From: HOWARD L. MULHERN :      mulhern-at-hub.tch.harvard.edu
Date: Fri, 01 Dec 2000 11:47:14 -0500
Subject: MSA CERTIFICATION

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does anyone know what happened to MSA Certification? I stopped at their
booth in August at MSA 2000 and was assured that things getting "back
on track" but so far I've heard nothing from them.

Howard Mulhern
mulhern-at-a1.tch.harvard.edu





From daemon Fri Dec 1 10:59:41 2000



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Fri, 01 Dec 2000 11:55:22 -0500
Subject: Raith(USA) Phone Number Correction

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

J. Faerber wrote:
==================================
To D. Sicard

Here are two adresses of Raith GmbH, in the USA and in Germany :
Raith INC.
6 Beech Road
Islip
NY 11751-4907
Phone (516)224-1764 { { {NOT CORRECT
Phone (631) 224-1764 { { {CORRECT
Fax (516)224-2620 { { {NOT CORRECT
FAX (631)224-2620 { { { {CORRECT
E-mail 73164.1330-at-compuserve.com
======================================
There has been an area code change and the old numbers absolutely won't work
!

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.2spi.com
########################
============================================








From daemon Fri Dec 1 11:02:39 2000



From: Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 01 Dec 2000 08:57:57 -0800
Subject: MIL-STD-883 Method 2018 - SEM Inspections

Contents Retrieved from Microscopy Listserver Archives
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Does anyone already have a prepared procedure according
to paragraphs 2.2 and 2.3 of MIL-STD-883E, Method 2018.3
they would share with me? What I am wondering is whether
this procedure is detailed (this knob, that button, etc.) or
more general in nature?

Based on the responses I did not receive from my earlier
post about quantitative measurement of SEM resolution,
it looks like paragraph 2 cannot be met. It states a
resolution of 250A or less. Semantically, I'm sure it
meant 250A or a lesser number. A resolution of 250A
or less would tend to mean a poorer resolution than
250A and hence a larger number. Probably picky
on my part.

Would appreciate any inputs regarding this MIL-STD.

Thanks,
gary g.




From daemon Fri Dec 1 12:18:28 2000



From: Tom Januszewski :      tom.januszewski-at-email.swmed.edu
Date: Fri, 01 Dec 2000 11:43:33 -0600
Subject: Lowicryl K4M processing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Listservers,

I've recently started lowicryl
(K4M) processing of mouse tissue. I
am having good success with
skeletal muscle, heart, kidney and
lung but stomach, spleen, pancreas
and adrenal had large holes and cut
poorly. In addition, a yeast pellet
that was resuspended in 2% agarose
prior to processing was not well
infiltrated.

Processing procedure:
3% paraformaldehyde/0.5% glut.
fixation for 60 minutes with
rotation (RT)
Wash 3X for tem minutes each with
PBS buffer
Dehydrate 30% ETOH for 30 min. at
0C
50% ETOH for 60
min. at -20C
80% ETOH for 60
min. at -35C
100% ETOH (freshly
opened bottle) for 60 min. at -35C
100% ETOH for 60
min. at -35C
ETOH/K4M 1:1 for 60
min. at -35C
ETOH/K4M 1:2 for 60
min. at -35C
K4M for 60 min.
at -35CC
K4M overnight at
-35C
K4M 6-8 hrs at -35C

Embed with fresh
resin
Specimens are constantly rotated in
freezer. All alcohols and resins
are prechilled prior to use. Resin
is mixeed by gently bubbling Argon
gas to dissolve initiator.
Polymerize at -35C overnight using
UV light
Raise temp to 0C and polymerize
additional two days with UV
Polymerize at room temp. for two
days using UV

Any thoughts on what I'm doing
wrong or things that I can change
would be greatly appreciated.

Tom Januszewski
Senior Electron Microscopist
UT Southwestern Medical Center at
Dallas
Dallas, TX 75390-9039
214-648-7291
FAX: 214-648-6408
Email:
tom.januszewski-at-utsouthwestern.edu




From daemon Fri Dec 1 13:04:07 2000



From: Gerroir, Paul J :      Paul.Gerroir-at-crt.xerox.com
Date: Fri, 1 Dec 2000 13:48:26 -0500
Subject: Looking for address of Company

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


December 1, 2000

I have an old business card (how old I don't know) for Raith. The contact
name is George Lanzarotta at (516) 293-0870. The address is (was?) 70C
Carolyn Boulevard, Farmingdale, NY 11735.

Good luck,
Paul

Paul J. Gerroir
Microscopy
Materials Characterization
Xerox Research Centre of Canada
2660 Speakman Drive
Mississauga, Ontario L5K 2L1

Phone: (905) 823-7091, ext. 216
FAX: (905) 822-7022
email: paul.gerroir-at-crt.xerox.com


-----Original Message-----
} From: Ladd Research [mailto:sales-at-laddresearch.com]
Sent: Thursday, November 30, 2000 4:26 PM
To: microscopy-at-sparc5.microscopy.com


Could anyone provide me with a phone number, e-mail or fax number for
the company "RAITH USA" It is a Germany Company and I thought there was
a distrbutor in the US

Thanks

D. Sicard
--

LADD RESEARCH
131 Dorset Lane
Williston, VT 05495 USA

web site http://www.laddresearch.com

TEL 1-800-451-3406 (US) or 1-802-878-6711 (anywhere)
FAX 1-802-878-8074
e-mail sales-at-laddresearch.com

Quality Since 1955



From daemon Fri Dec 1 19:11:28 2000



From: Rick Harris :      raharris-at-ucdavis.edu
Date: Fri, 01 Dec 2000 17:03:47 -0800
Subject: Pt evaporation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Just got a new vacuum evaporator after many years of using our Balzers 400
for the same purpose. Course now we don't have e guns and must use a
little tungsten basket and a bit of Pt wire. I did this many years ago but
now I am having a problem I don't recall encountering back then. The
tungsten basket breaks just as the wire melts. I suspect the energy on
phase transition is cooling the tungsten and causing it to break but I
could be way off there. Anyone else see this problem and know the solution?

TIA


Rick A. Harris, Director
Microscopy and Imaging Facility
Section of Molecular and Cellular Biology
1241 Life Sciences Addition
University of California
Davis, CA
530 752 2914
530 754 7536 fax
http://katie.ucdavis.edu
raharris-at-ucdavis.edu




From daemon Fri Dec 1 20:18:14 2000



From: mtl :      mtl-at-njcc.com
Date: Fri, 01 Dec 2000 21:16:04 -0500
Subject: Re: Thesis question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Giselle
You probably should check out the Journal of Fuel or "Fuel". "Carbon"
occasionally has articles linking the mineral content of the feedstock with the
resultant char. The Northern Carbon Research Laboratories, U. of
Newcastle-upon-tyne used to publish regularly in this field. Penn. State also
has a very active Fuel Science Dept.
Good Luck.

J. Roy Nelson, Ph.D.
Material Testing Laboratory
mtl-at-njcc.com

giselle melville wrote:

}
} Good afternoon
}
} My name is Giselle. I am a graduate student working on my thesis. My
} thesis topic is "Methods of preparation of petrographic thin sections for
} Electron Microscopy". I have a collection of thin sections (chert) and I am
} preparing the sections for analysis by a TEM 1200. The thin sections are
} initially prepared by methods Ultramicrotomy and Ion Milling. TEM
} parameters used will be electron diffraction and elemental composition to
} obtain identification of the mineral and microstructures. I need more
} information (references) on recent literature (1998-2000) on my subject or
} similiar work done using these methods for analyis by TEM.
}
} I need your help!!!
}
} Thank you for your time.




From daemon Sat Dec 2 02:00:19 2000



From: Gordon Couger :      gcouger-at-couger.com
Date: Sat, 2 Dec 2000 01:53:26 -0600
Subject: Ask-A-Microscopist DIC vs phase for viewing living organisms

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am helping a friend find a scope. I came across a Zetopan that may fit
his budget. I use a Nachet DIC for viewing living organisms and find it
very satisfactory. Could anyone help him with the versatility of a DIC
Zetopan. I know my Nachett works as a bright field, DIC and sort of
polarizing scope lacking a rotating stage and 1/4 and 1/2 wave plates. It
is the scope I use all the time. Unless I am doing darkfield.

Having never been around a Zetopan I can't really answer his questions.

Thanks
Gordon
Gordon Couger gcouger-at-couger.com
Stillwater, OK www.couger.com/gcouger


} } I my quest to find a better 'scope I have come across (with the help
} } of one of the list members) a Reichert Zetopan DIC scope that *might*
} } be available at in my price range. Previously I was just looking for
} } a nice phase contrast job. I have worked with phase before but not
} } DIC and would like to solicit other opinions as to whether this is
} } the right scope for me. This is going to pretty much blow my budget
} } so I want to be sure.
} }
} } I will be using this primarily for observing living organisms
} } (protozoa, algae, etc). I have been looking at a lot of photos of
} } protozoa taken using Nomarski DIC. While the photos are striking, I
} } keep asking myself "but what does it really look like". The problem
} } is the false 3D effect. I'm never sure what the part of the shape is
} } real and what is generated. Doesn't this ever bother you?
} }
} } I have been told that it is possible to adjust out the 3-D effect but
} } I'm not sure what kind of image you are left with. I would assume
} } something in-between bright field and DIC. Anyone have any experience
} } with this?
} }
} } I think if the Zetopan is unable to easily switch between a DIC image
} } and a bright (or dark) field image then I may pass on it. I need the
} } more traditional illumination as well to see what the real shape is.
} } It really makes the Zetopan too special purpose for someone like
} } myself. I think I would probably be happier with a less
} } sophisticated scope that can do phase, bright, and dark field (and
} } maybe oblique lighting as well).
} }
} } I would appreciate any thoughts you all may have.
} }
} } Bill
} } --
} } Bill Tschumy
} } Otherwise -- Austin, TX
} } bill-at-otherwise.com





From daemon Sat Dec 2 10:30:43 2000



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Sat, 02 Dec 2000 11:24:29 -0500
Subject: Pt evaporation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Rick A. Harris wrote:
================================================================
Just got a new vacuum evaporator after many years of using our Balzers 400
for the same purpose. Course now we don't have e guns and must use a
little tungsten basket and a bit of Pt wire. I did this many years ago but
now I am having a problem I don't recall encountering back then. The
tungsten basket breaks just as the wire melts. I suspect the energy on
phase transition is cooling the tungsten and causing it to break but I
could be way off there. Anyone else see this problem and know the solution?
================================================================
There really is no perfect "solution". The precious group metals tend to
alloy quite readily with tungsten, Au and Pd very quickly, and I believe Pt
is quite similar in behavior.

You can reduce the magnitude of the "problem" by making sure that when you
install the tungsten basket, the wire itself is under zero stress. This
might mean a bit more time adjusting the holders and posts than you might be
doing now. Also, ball up the Pt wire and press into the very bottom of the
basket, this will enable you to get the most evaporated in the shortest
possible time period, before the wire basket breaks. And you want to use
more of a "flash" evaporation than a long slow continuous kind of
evaporation.

Another approach, but one we would not recommend, is to use a thicker wire
basket but many EM samples could be heat damaged from the additional radiant
heat. 20 mil wire is our recommended basket wire diameter.

Hope this information will be helpful.

Disclaimer: SPI Supplies manufactures tungsten wire baskets for vacuum
evaporation.

Chuck

============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.2spi.com
########################
============================================




From daemon Sat Dec 2 21:40:12 2000



From: Ronald Austin :      rla-at-mindspring.com
Date: Sat, 2 Dec 2000 21:28:05 -0600
Subject: a data base program

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does anyone out there in microscopy land know of a computer database program
( preferably PC) that has the scientist in mind and not the business man. I
have Excel, Access, and Project 2000 but they are orientated to calculate
dollars and cent not milliliters and millimeters if you know what I mean.

Ron Austin
Dept of Pathology
LSU Medical Center
Shreveport, LA
rla-at- mindspring.com
318-675-4557




From daemon Sun Dec 3 06:21:40 2000



From: Tobias Baskin :      BaskinT-at-missouri.edu
Date: Sun, 3 Dec 2000 14:53:37 -0600
Subject: Re: Ask-A-Microscopist DIC vs phase for viewing living organisms

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


yes I am using databases from dbase II on cp/m systems

ricardo

----- Original Message -----
} From: "Vr. Richard Bejsak-Collorado-Mansfeld" {ricardo-at-ans.com.au}
To: "Ronald Austin" {rla-at-mindspring.com}
Sent: Sunday, December 03, 2000 11:16 PM


Greetings,
Nomarski optics are widely considered appropriate for viewing
living cells. While it is true that the "3-D" look might or might not
be present in the cell (more about that later), one must remember
that in phase contrast there is the famous "halo", representing an
out of focus reverse-contrast image that certainly is not present in
the cell.

Nomarski optics provide contrast by means of a gradient in
optical path (= sample thickness times refractive index). That is
anywhere in the sample where the local gradient in optical path is
different than the background (which typically would have a zero
gradient) the intenstity of the image is different than the
background. When the gradient is positive the intensity is brighter
than background, when the gradient is negative then the intensity is
darker than background. The most obvious gradients of optical path
for a living cell suspended in an aqueous medium is the change in
thickness that happens at the cell's edges: at one edge the cell is
getting thicker (= postive gradient, hence bright) and at the
opposite edge the cell gets thinner (= negative gradient, hence
dark). Consquently, the topographic appearance does relate to the
sample in a real, predictable way.

Nomarski optics can be harder to adjust properly than phase
contrast. In phase, you have the phase ring in the condenser and this
has to be centered with the phase ring in the objective. That's all
you have to do. For Nomarski, you have to have first crossed
polarizers, you have to know where they are and be able to rotate at
least one of them. THen, the system also has two Wallason prisms, one
in the condenser and one above the objective. The Wallaston prism has
an optical axis and this axis of each Wallaston must be parallal and
at 45 degrees to the analyzer/polarizer alignment. Different
implementations of Nomarski differ vastly in the accessibility of the
elements and the ease of their aligment. In the good designs, it is
no trouble at all. But of course, one should know where each one is,
what it does and how it should be aligned.

Changing between nomarki and brightfied is no trouble, you
just remove the analyzer or polarizer from the optical path.

One important fact about Nomarski arises from the optical
axis of the Wallastons. This defines the direction used to assess the
gradient in optical path. The optics cannot detect gradients that run
perpendicular to the Wallaston's optical axis. This means that
features revealed in the image depend on the orientation of the cell.
A rotatable stage is therefore very desirable. What is more,
depending on your purpose, and the types of cells your are looking
at, the directional properties of Nomarski will be more or less of a
problem.

I have no experience with a Zetopan. If at all possible have
demonstration before you buy. Finally, in many scopes you can
exchange conderser elements so that you can do either phase or
Nomarski. Thus, you could expand your repertoire later, as needs
arise. I would be useful to find out if the Zetopan lets you do that.

Hope this helps,
Tobias Baskin

}
} I am helping a friend find a scope. I came across a Zetopan that may fit
} his budget. I use a Nachet DIC for viewing living organisms and find it
} very satisfactory. Could anyone help him with the versatility of a DIC
} Zetopan. I know my Nachett works as a bright field, DIC and sort of
} polarizing scope lacking a rotating stage and 1/4 and 1/2 wave plates. It
} is the scope I use all the time. Unless I am doing darkfield.
}
} Having never been around a Zetopan I can't really answer his questions.
}
} Thanks
} Gordon
} Gordon Couger gcouger-at-couger.com
} Stillwater, OK www.couger.com/gcouger
}
}
} } } I my quest to find a better 'scope I have come across (with the help
} } } of one of the list members) a Reichert Zetopan DIC scope that *might*
} } } be available at in my price range. Previously I was just looking for
} } } a nice phase contrast job. I have worked with phase before but not
} } } DIC and would like to solicit other opinions as to whether this is
} } } the right scope for me. This is going to pretty much blow my budget
} } } so I want to be sure.
} } }
} } } I will be using this primarily for observing living organisms
} } } (protozoa, algae, etc). I have been looking at a lot of photos of
} } } protozoa taken using Nomarski DIC. While the photos are striking, I
} } } keep asking myself "but what does it really look like". The problem
} } } is the false 3D effect. I'm never sure what the part of the shape is
} } } real and what is generated. Doesn't this ever bother you?
} } }
} } } I have been told that it is possible to adjust out the 3-D effect but
} } } I'm not sure what kind of image you are left with. I would assume
} } } something in-between bright field and DIC. Anyone have any experience
} } } with this?
} } }
} } } I think if the Zetopan is unable to easily switch between a DIC image
} } } and a bright (or dark) field image then I may pass on it. I need the
} } } more traditional illumination as well to see what the real shape is.
} } } It really makes the Zetopan too special purpose for someone like
} } } myself. I think I would probably be happier with a less
} } } sophisticated scope that can do phase, bright, and dark field (and
} } } maybe oblique lighting as well).
} } }
} } } I would appreciate any thoughts you all may have.
} } }
} } } Bill
} } } --
} } } Bill Tschumy
} } } Otherwise -- Austin, TX
} } } bill-at-otherwise.com

--
_ ____ __ ____ Tobias I. Baskin
/ \ / / \ / \ \ 109 Tucker Hall
/ / / / \ \ \ Biological Sciences
/_ / __ /__ \ \ \__ University of Missouri
/ / / \ \ \ Columbia, MO USA
/ / / \ \ \ 65211-7400
/ / ___ / \ \__/ \ ____ voice: 573-882-0173
fax: 573-882-0123



From daemon Sun Dec 3 15:30:59 2000



From: Caroline Schooley :      schooley-at-mcn.org
Date: Sun, 3 Dec 2000 13:17:14 -0800
Subject: Microscopy education

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I'm pleased to announce that a revised, up-to-date version of "Children's
Microscopy: a Bibliography" will be mailed as a supplement to the December
issue of Microscopy Today; watch for it. It has brief reviews and ordering
information on books, videos, CD-ROMs and websites. If you can't use it
yourself, PLEASE give it to a teacher, or a parent, or a Scout leader, or a
librarian - anyone who can use it to introduce children to the microworld.

If you aren't a Microscopy Today subscriber {microtoday-at-mindspring.com} you
can read the same text soon on Project MICRO's website (URL below). But
it's a tightly packed 16 pages, so it will be easier to browse the printed
version.

Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html





From daemon Sun Dec 3 16:36:55 2000



From: Raymond Bennett :      rbennett-at-hortresearch.co.nz
Date: Mon, 4 Dec 2000 11:31:32 +1300
Subject: Change of Address

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Howdy;

Our email address has changed to
rbennett-at-hortresearch.co.nz


Thanks

Raymond Bennett
Keith Williamson Electron Microscope Unit
Hort+Research
Private Bag 11030
Palmerston North
NEW ZEALAND



From daemon Sun Dec 3 23:08:55 2000



From: Ronald Austin :      rla-at-mindspring.com
Date: Sun, 3 Dec 2000 23:00:39 -0600
Subject: a data base program

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I wish to thank all of you who responded to my question about a database
program. I shall look into the various suggestion in the near future.

Ron Austin
Dept of Pathology
LSU Medical Center
Shreveport, LA




From daemon Mon Dec 4 08:38:08 2000



From: Robert Price :      spike-at-auburn.edu
Date: Mon, 4 Dec 2000 08:30:31 -0600
Subject: Need help with EDS detector

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We are looking for a second hand EDS detector. Please email me off list.
Thanks,
Robert
C.A.V.E.
Auburn University




From daemon Mon Dec 4 09:26:35 2000



From: Rachel Spicer :      spicer-at-oeb.harvard.edu
Date: Mon, 04 Dec 2000 10:21:49 -0500
Subject: lignin autofluorescence

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello -

Does anyone out there have suggestions on histochemical stains (or other
techniques) to reduce the autofluorescence of lignin? I'm using DAPI
staining on wood (xylem) sections, which have highly lignified cell walls,
and I would like to reduce background fluorescence. Thanks.

Rachel

******************************************
Rachel Spicer
Biological Laboratories 3119
Organismic and Evolutionary Biology
Harvard University
16 Divinity Avenue
Cambridge, MA 02138

(617) 496-3580 (phone)
(617) 496-5854 (fax)
spicer-at-oeb.harvard.edu
******************************************




From daemon Mon Dec 4 09:28:14 2000



From: Walck, Scott D. :      walck-at-ppg.com
Date: Mon, 4 Dec 2000 10:26:06 -0500
Subject: RE: a data base program

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


It is relatively easy to program in Microsoft's Visual Basic 6 using
databases. It is one of that program's strengths. You can tailor any
properties that you want. It is easy to set it up to be compatible with
Microsoft's Access, but you can have it set up for any ODBC database. You
can actually set up a working database program by just using the Form Wizard
that comes with VB6.


-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)



-----Original Message-----
From: Ronald Austin [mailto:rla-at-mindspring.com]
Sent: Saturday, December 02, 2000 10:28 PM
To: Microscopy Society of America
Subject: a data base program


---------------------------------------------------------------
---------
The Microscopy ListServer -- Sponsor: The Microscopy Society
of America
To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
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---------------------------------------------------------------
--------.


Does anyone out there in microscopy land know of a computer
database program
( preferably PC) that has the scientist in mind and not the
business man. I
have Excel, Access, and Project 2000 but they are orientated
to calculate
dollars and cent not milliliters and millimeters if you know
what I mean.

Ron Austin
Dept of Pathology
LSU Medical Center
Shreveport, LA
rla-at- mindspring.com
318-675-4557





From daemon Mon Dec 4 09:45:56 2000



From: Philip Koeck :      philip.koeck-at-csb.ki.se
Date: Mon, 04 Dec 2000 16:44:36 +0100
Subject: TEM resolution

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hallo everybody,

I've started wondering about what actually the point resolution of
a TEM in phase-contrast imaging is.

} From Raleigh's criterion I would conclude that for a typical 120 kV
TEM with a numerical aperture of about 20 mrad distances larger than
about 2 Angstrom or 'density waves' with a wavelength longer than
2 Angstrom simply cannot be resolved.

In Scherzer defocus the Phase Contrast Transfer Function (PCTF) has its
first zero at about the same resolution but there seems to be
information
at higher frequencies (even if it is inverted in contrast).

Which is the true resolution limit?

Yours sincerely,

Philip

--
Philip Koeck
Karolinska Institutet
Dept. of Bioscience
Novum
S-14157 Huddinge
Sweden
Tel.: +46-8-608 91 86
Fax.: +46-8-608 92 90
Email: Philip.Koeck-at-csb.ki.se
http://www.csb.ki.se/em



From daemon Mon Dec 4 10:49:11 2000



From: Ann Dvorak :      advorak-at-caregroup.harvard.edu
Date: Mon, 4 Dec 2000 11:55:34 -0500
Subject: tricks for better ribbons?

Contents Retrieved from Microscopy Listserver Archives
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Trying to cut serial sections of Spurr for 3D reconstruction. Anyone know
of any tricks to make the sections stay together in long ribbons? Ellen
Morgan



From daemon Mon Dec 4 13:28:57 2000



From: Michael O'Keefe :      MAOKeefe-at-lbl.gov
Date: Mon, 04 Dec 2000 11:26:15 -0800
Subject: Re: TEM resolution

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Philip,

In the field of high-resolution transmission electron microscopy, the
"resolution" is defined as the first zero in the phase contrast transfer
function (PCTF) at Scherzer (or optimum) defocus as you point out. This
definition is chosen because any higher-frequency information is phase
inverted (as you also point out). However, it results in defining the
resolution in terms of the spatial frequency at which transfer goes to zero,
rather than in terms of the highest transferred spatial frequency.

I have suggested a definition in which the resolution is defined at the 70%
pass limit within the Scherzer passband, giving d = 0.67 Cs^1/4 lambda^3/4
at an "extended" optimum defocus of sqrt(1.5Cs.lambda) instead of Scherzer's
d = 0.707 Cs^1/4 lambda^3/4 at an optimum defocus of sqrt(Cs.lambda).
Current practice seems to use the extended defocus with the crossover
(zero-transfer) frequency to get d = 0.64 Cs^1/4 lambda^3/4 at the
"extended" optimum defocus.

The information beyond the Scherzer cross-over (zero-transfer) frequency can
be utilized to provide structural information. Because of the misphasings,
this information is difficult to interpret directly. Originally, the way to
get at this higher-frequency (smaller-spacing) information was to compare
focal series of experimental images with images simulated from possible
models. In this way, we "saw" the small tunnels in Nb12O29 (with a
2.5Angstrom spacing) with a JEOL 100B (Scherzer resolution of 3.5A) in a
focal series obtained by Sumio Iijima (see "Resolution-limiting effects in
electron microscope images", G.R. Anstis and M.A. O'Keefe, In 34th Ann.
Proc. EMSA, Miami Beach (1976) 480-481). I used HRTEM image simulation
programs such as my SHRLI (simulated high-resolution lattice image) code, or
my later interactive TEMPaS (TEM processing and simulation) code (ported to
the Mac as MacTempas) with great success, but only when the model was known
or limited to several possible ones (for example "Inversion domains in GaN
grown on sapphire", L.T. Romano, J.E. Northrup and M.A. O'Keefe, Appl. Phys.
Lett. 69 (1996) 2394-2396). Currently, post-Scherzer information can be
accessed by image reconstruction algorithms from focal series, or tilt
series, or holography. I use the Philips/Brite-Euram software for
focal-series reconstruction by Coene and Thust in my one-Angstrom microscope
(OAM) project to extend the resolution of a modified Philips CM300FEG/UT
from its native resolution of 1.7Angstrom to a super-resolution of
0.89Angstrom that can image the "dumbbells" in [110] diamond (see “The NCEM
One-Ångstrom Microscope project reaches 0.89Å resolution”, M. A. O'Keefe in
58th Ann. Proc. MSA, Philadelphia, Pennsylvania (2000) 1192-1193).

For the equations that belong with a discussion of resolution, see
"Resolution in high-resolution electron microscopy", M.A. O'Keefe,
Ultramicroscopy 47 (1992) 282-297. For a (simple) explanation of the use of
focal-series to obtain super-resolution see "Push TEM limits with
super-resolution", Michael A. O'Keefe (1999), R&D Magazine October 1999, p79
or {http://www.rdmag.com/archives/basics/10microscopy.htm} . For details of
the Philips/Brite-Euram software for focal-series reconstruction by Coene
and Thust, see W.M.J. Coene, A. Thust, M. Op de Beeck and D. Van Dyck,
Ultramicroscopy 64 (1996) 109-135 and A. Thust, W.M.J. Coene, M. Op de Beeck
and D. Van Dyck, Ultramicroscopy 64 (1996) 211-230.
In the above, I have dealt only with linear terms in the transfer of
information from the specimen (more properly, the exit-surface wave from the
specimen) into the HREM image. Non-linear terms can give rise to
even-higher spatial frequencies in the image. However, the question of
whether these can be termed "resolution" is doubtful (see
"Resolution-damping functions in non-linear images", M.A. O'Keefe, in 37th
Ann. Proc. EMSA, San Antonio, Texas (1979) 556-557).

Mike O'Keefe


Philip Koeck wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hallo everybody,
}
} I've started wondering about what actually the point resolution of
} a TEM in phase-contrast imaging is.
}
} } From Raleigh's criterion I would conclude that for a typical 120 kV
} TEM with a numerical aperture of about 20 mrad distances larger than
} about 2 Angstrom or 'density waves' with a wavelength longer than
} 2 Angstrom simply cannot be resolved.
}
} In Scherzer defocus the Phase Contrast Transfer Function (PCTF) has its
} first zero at about the same resolution but there seems to be
} information
} at higher frequencies (even if it is inverted in contrast).
}
} Which is the true resolution limit?
}
} Yours sincerely,
}
} Philip
}
} --
} Philip Koeck
} Karolinska Institutet
} Dept. of Bioscience
} Novum
} S-14157 Huddinge
} Sweden
} Tel.: +46-8-608 91 86
} Fax.: +46-8-608 92 90
} Email: Philip.Koeck-at-csb.ki.se
} http://www.csb.ki.se/em

--------------------------------------------------------
Your mail has been rejected for the following reason(s):
--------------------------------------------------------
OK, Nestor -- this time it's in plain text.
-Mike




From daemon Mon Dec 4 14:24:14 2000



From: Leona Cohen-Gould :      lcgould-at-mail.med.cornell.edu
Date: Mon, 4 Dec 2000 15:23:19 -0500
Subject: Re: tricks for better ribbons?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 11:55 AM -0500 12/4/00, Ann Dvorak wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

************************
Hi Ellen,
I vaguely remember reading something about coating the sides of the
block that will become the leading and trailing faces with something
slightly tacky (like the adhesive dissolved off of 3M Magic Scotch
tape). Once that has dried, the edges of the sections are supposed
to stick together. I've never actually tried it, and I wonder how
you do manage to separate the sections where you need to.

Does anyone out there remember this? Has anyone tried it?

Lee

Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175



From daemon Mon Dec 4 14:36:31 2000



From: John J. Bozzola :      bozzola-at-siu.edu
Date: Mon, 4 Dec 2000 14:33:04 -0600
Subject: MSA Archivist position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The Microscopy Society of America is seeking an Archivist to manage
the historical documents of our Society. This position is appointed
by MSA Council for a period of three years.

We are looking for someone who is experienced in the storage and
cataloging of (primarily) paper documents. Although this is a
voluntary position (non-paying), the Society would provide funding
for expendibles and day-to-day operations.

If interested (or if you know of a qualified person), please contact me at:

--
##############################################################
John J. Bozzola, Ph.D., Director
I.M.A.G.E. (Integrated Microscopy & Graphics Expertise)
750 Communications Drive - MC 4402
Southern Illinois University
Carbondale, IL 62901 U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
##############################################################



From daemon Mon Dec 4 14:49:05 2000



From: Schumacher, Elaine :      efschuma-at-uop.com
Date: Mon, 4 Dec 2000 14:45:42 -0600
Subject: RE: Tricks for Better Ribbons

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all,

A predecessor in our laboratory came back from the MSA conference a few
years back with a sample from a vendor of something called Tacki Wax, which
was supposed to serve this purpose. I think the idea was that the sections
would be gently held together, but not so strongly that you couldn't work
them apart as needed while collecting them. I've never tried it myself, as
I don't need to do serial sectioning, but having a product name to go by
might help your search.

Elaine Schumacher
UOP
25 East Algonquin Rd.
Des Plaines, IL 60017-5017
phone: 847-391-3403
fax: 847-391-3719
efschuma-at-uop.com



From daemon Mon Dec 4 15:42:46 2000



From: James Pawley :      jbpawley-at-facstaff.wisc.edu
Date: Mon, 4 Dec 2000 14:18:55 -0600
Subject: Information about the 2001 UBC "Live Cell" course

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello all,

I am pleased to announce that the Live-cell Course continues to grow
and prosper. Over 130 students from 23 countries have taken the
course over the last 5 years.

Now I am organizing the next 11-day summer course on "3D Microscopy
of Living Cells" for June of 2001. As in the past it will be at the
University of British Columbia, in Vancouver, BC. The Fifth Workshop
on 3D Image Processing will be held June 30 - July 2.

Please check out http://www.cs.ubc.ca/spider/ladic/course/bulletin.html
if you would like to have a feeling for last years's course.

The information for 2001 is below.

Thanks for your help,

Jim Pawley


Sixth Annual INTERNATIONAL 11-Day Short Course on

3D Microscopy of Living Cells
June 18 - 28, 2001



Fifth, Post-course Workshop on

3D Image Processing,
June 30 - July 2, 2001



Organized by Prof. James Pawley,
(University of Wisconsin-Madison)
(SEE ADDRESS AT END OF MESSAGE)


in association with Dr. Elaine Humphrey
UBC BioSciences Microscopy Facility:
University of British Columbia
Vancouver, BC, Canada


DATES

Applications must be received by March 1, 2001
Deposit due April 15, 2001
Registration 5:00 - 7:00 PM Sunday, June 17, 2001
First Lecture 7:30 PM Sunday, June 17, 2001
Live-cell Course ends, noon Thursday, June 28, 2001
3D Image Processing Course, June 30, - July 2, 2001


APPLICATIONS DUE BY MARCH 1, 2001


APPLICATIONS
Applicants must complete a questionnaire to assess knowledge level,
field of interest and proposed personal, live-cell, project.
Enrollment will be limited to about 24 participants (exact number
depends on number of 3D Systems available). Selection will be made
on the basis of background and perceived need. Those without
previous LM experience will be provided with access to basic texts to
read before the course begins. Application forms requesting
information on field of interest and level of experience may be
down-loaded from the WWW site at
http://www.cs.ubc.ca/spider/ladic/course/reg1.htm, or obtained from:

Dr. Elaine Humphrey,
Biosciences EM Facility
Biosciences Building
Univ. of British Columbia
6270 University Blvd.
Vancouver, BC, V6T-1Z4

Additional information is available from:
http://www.cs.ubc.ca/spider/ladic/course/bulletin.html

We expect to have at least 11, 3D microscope workstations for student
use and there will be an international faculty of 17.

Application deadlines:

Application forms must be received for screening by March 1, 2000.
Successful applicants will be notified by April 1, and a deposit of
50% must be received by April 15, 2000. In general, refunds of the
deposit will not be possible. The remainder is due before
Registration.


3D Course tuition (includes lunches and snacks): $2150 (US)
Workshop Tuition (includes lunches and snacks): $850 (US)

Room / board about $40/day (US)

I hope that this includes all of the information that you need, but
if not, please get back to me.
--
****************************************
Prof. James B. Pawley, Ph. 608-263-3147
Room 223, Zoology Research Building, FAX 608-265-5315
1117 Johnson Ave., Madison, WI, 53706 JBPAWLEY-at-FACSTAFF.WISC.EDU
"A scientist is not one who can answer questions
but one who can question answers."
Theodore Schick Jr., Skeptical Enquirer, 21-2:39



From daemon Mon Dec 4 16:48:33 2000



From: MICHAEL DELANNOY :      delannoy-at-mail.jhmi.edu
Date: Mon, 4 Dec 2000 17:41:59 -0500 (EST)
Subject: Help with 3d software

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Mike,

Thanks for offering to put my 3D rendering question on the
discussion group.
My lab currently acquires and processes images using IPlab and a
SPOT RT camera. We have MicroTome by VayTek for deconvolution, but this
isn't much good without 3D rendering. Our primary application will be
measuring the volume of nuclei and cells which have been fluorescently
stained. Which 3D programs for a PC might work well for this?

Thanks,
Darren








From daemon Mon Dec 4 16:51:24 2000



From: MICHAEL DELANNOY :      delannoy-at-mail.jhmi.edu
Date: Mon, 4 Dec 2000 17:48:00 -0500 (EST)
Subject: chemlumanesence

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hello all,
Any one with on advice on who in the Baltimore area can do
chemlumanesence (spelling?) on live cells, realtime and time series?
What equipment do we need, which company or labs are doing this.

thanks,
Mike D.




From daemon Mon Dec 4 17:14:58 2000



From: Mike Bode :      mb-at-Soft-Imaging.com
Date: Mon, 4 Dec 2000 16:08:10 -0700
Subject: TEM resolution

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


OK,

I'll take a stab at this. Please correct me if I am wrong:

The point resolution refers to the resolution limit of a TEM for (as the
name implies) two point like objects. A pointlike object is mathematically a
delta-function and has a Fourier spectrum that is constant for all
frequencies. So the question becomes: how many of the Fourier components can
be transmitted without changing them, which in turn determines the minimum
size of the object. The answer is then basically given by the first zero of
the CTF. Until this point, the spatial frequencies are transmitted with the
same phase and almost the same amplitude. For higher frequencies, the
amplitude of the CTF oscillates, so that no reliable transmission can be
achieved. The setting, where the first zero is furthest out in Fourier space
is the Scherzer defocus. Thus the point resolution is determined by the
first zero of the CTF at Scherzer defocus.

Line resolution refers to a periodic structure with very few Fourier
components. It is therefore not necessary to transmit the entire Fourier
spectrum, but only one specific component. Thus by changing the CTF through
changing the focus, one can find a setting, where that specific frequency is
transmitted and shows up in the image. The question therefore becomes: How
well can I detect the structure. This is then in principle determined by the
amplitude of the CTF. So the line resolution is then determined by the CTF
envelope going below a certain threshold (I believe it is 20%, but I am not
sure). That threshold is of course more or less arbitrary, but should give a
reasonable estimate of the line resolution and allow comparison of the
microscope resolutions.

Disclaimer: Please use this with caution. I have not been involved with EM
theory for a few years. For a good account of high-resolution TEM, check out
the book(s) by John Spence.

Michael Bode


Michael Bode, Ph.D.
Soft Imaging System Corp.
1675 Carr St., #105N
Lakewood, CO 80215
===================================
phone: (888) FIND SIS
(303) 234-9270
fax: (303) 234-9271
email: mailto:info-at-soft-imaging.com
web: http://www.soft-imaging.com
===================================



-----Original Message-----
} From: Philip Koeck [mailto:philip.koeck-at-csb.ki.se]
Sent: Monday, December 04, 2000 8:45 AM
To: 3dem list; MSA mailing list


Hallo everybody,

I've started wondering about what actually the point resolution of
a TEM in phase-contrast imaging is.

} From Raleigh's criterion I would conclude that for a typical 120 kV
TEM with a numerical aperture of about 20 mrad distances larger than
about 2 Angstrom or 'density waves' with a wavelength longer than
2 Angstrom simply cannot be resolved.

In Scherzer defocus the Phase Contrast Transfer Function (PCTF) has its
first zero at about the same resolution but there seems to be
information
at higher frequencies (even if it is inverted in contrast).

Which is the true resolution limit?

Yours sincerely,

Philip

--
Philip Koeck
Karolinska Institutet
Dept. of Bioscience
Novum
S-14157 Huddinge
Sweden
Tel.: +46-8-608 91 86
Fax.: +46-8-608 92 90
Email: Philip.Koeck-at-csb.ki.se
http://www.csb.ki.se/em



From daemon Mon Dec 4 17:16:29 2000



From: Jon Ekman :      jekman-at-csd.uwm.edu
Date: Mon, 4 Dec 2000 17:14:59 -0600
Subject: Re: tricks for better ribbons?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all,


For Light microscopy, we used a mixture of Weldwood contact
cement from the local hardware store and toluene mixed 50/50. Place
it on the leading and trailing edges of the block using a pin. We used
a tool to pick up the sections I don't remember its name. It looked
like a small metal trough.


For EM I used to make the block face area as small as possible
focusing on a specific area of interest. Then I would cut the the
leading and trailing edges parallel and then put the block on the ultra
microtome and polish the edges with a glass knife set on zero
advance. If the leading and trailing edges are smooth the sections
stick together when coming off the block. I would make groups of
ten sections. pick up with a tungsten wire loop (premade loops work
better from EM venders of your choice) and place on a formvar
coated slot grid.


Good Luck


Jon








{color} {param} 0100,0100,0100 {/param} On 4 Dec 00, at 15:23, Leona Cohen-Gould wrote:


{color} {param} 7F00,0000,0000 {/param} } ----------------------------------------------------------------------

} -- The Microscopy ListServer -- Sponsor: The Microscopy Society of

} America To Subscribe/Unsubscribe -- Send Email to

} ListServer-at-MSA.Microscopy.Com On-Line Help

} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html

} ----------------------------------------------------------------------

} -.

}

}

} At 11:55 AM -0500 12/4/00, Ann Dvorak wrote:

} } ---------------------------------------------------------------------

} } --- The Microscopy ListServer -- Sponsor: The Microscopy Society of

} } America To Subscribe/Unsubscribe -- Send Email to

} } ListServer-at-MSA.Microscopy.Com On-Line Help

} } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html

} } ---------------------------------------------------------------------

} } --.

} }

} }

} } Trying to cut serial sections of Spurr for 3D reconstruction. Anyone

} } know of any tricks to make the sections stay together in long

} } ribbons? Ellen Morgan

}

} ************************

} Hi Ellen,

} I vaguely remember reading something about coating the sides of the

} block that will become the leading and trailing faces with something

} slightly tacky (like the adhesive dissolved off of 3M Magic Scotch

} tape). Once that has dried, the edges of the sections are supposed to

} stick together. I've never actually tried it, and I wonder how you do

} manage to separate the sections where you need to.

}

} Does anyone out there remember this? Has anyone tried it?

}

} Lee

}

} Leona Cohen-Gould, M.S.

} Sr. Staff Associate

} Director, Electron Microscopy Core Facility

} Manager, Optical Microscopy Core Facility

} Joan & Sanford I. Weill Medical College

} of Cornell University

} voice (212)746-6146

} fax (212)746-8175

}

}



{nofill}
Jon Ekman
Associate Research Specialist
Deptartment of Biological Sciences
University of Wisconsin-Milwaukee
phone W:414.229.6471
Web1 http://www.graffitimasters.com
Web2 http://www.uwm.edu/~jekman



From daemon Mon Dec 4 17:54:48 2000



From: Walck, Scott D. :      walck-at-ppg.com
Date: Mon, 4 Dec 2000 18:49:23 -0500
Subject: profiler images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I would like to know whether there are any commercial labs that can provide
up to 3000 um x 3000 um contact profilometry image maps such as the Veeco
Dektak Profiler or the KLA-Tencor Profiler can provide.

-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)





From daemon Mon Dec 4 18:19:27 2000



From: Linda Barthel :      barthel-at-umich.edu
Date: Mon, 4 Dec 2000 18:14:59 -0600
Subject: Serial sections.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Go to Rivlin and Raymond, 1987, Journal of Neuroscience Methods, 20:23-33,
also see Raymond and Rivlin, 1987, Developmental Biology, 122:120-138.
There you will find reference to the technique originally published by W.
Fahrenbach, 1984, J. Electron. Microsc. Tech., 1:387-398. In the Rivlin
paper you will find that a solution of Weldwood cement (rubber cement) was
applied to the top and bottom edges of the trapezoid block face. This glue
can be use undiluted or diluted in the appropriate solvent to obtain the
desired workable concentration.

Linda Barthel, M.S.
Research Associate II
University of Michigan
Department of Cell and Developmental Biology
4607 Medical Science Building II
Ann Arbor, MI 48109-0616

(734) 764-7476

http://www-personal.umich.edu/~praymond





From daemon Mon Dec 4 20:26:38 2000



From: COURYHOUSE-at-aol.com
Date: Mon, 4 Dec 2000 21:19:58 EST
Subject: wanted books and stuff on science fighting crime, American Journal of Police Sc

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Looking for back issues or bound volumes of
American Journal of Police Science, edited by
the Scientific Crime Detection Laboratory,

Also will purchase books showing how to use
bullet comparison microscopes, finger print cameras,
hair and fiber comparison microscopes, etc., etc.

Think of it this way, if it is using science, medicine and gadgets to solve
crime we need artifacts,ephemera and books for our museum display.

Will pay cash or trade goodies!

Thanks Ed Sharpe, Archivist for SMECC



From daemon Mon Dec 4 23:58:46 2000



From: charles j day :      wa5ekh-at-juno.com
Date: Sat, 2 Dec 2000 23:49:53 -0500
Subject: Polymers TEM/SEM position in Texas

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


There may be a position opening up in the Dallas/Ft. Worth Area for an
experienced microscopist with polymers-TEM experience. If you might be
interested please send me a resume.
Jeff Day/wa5ekh-at-juno.com
________________________________________________________________
GET INTERNET ACCESS FROM JUNO!
Juno offers FREE or PREMIUM Internet access for less!
Join Juno today! For your FREE software, visit:
http://dl.www.juno.com/get/tagj.



From daemon Tue Dec 5 08:34:46 2000



From: Ed Kurz :      ekurz-at-mail.ims.uconn.edu
Date: Tue, 05 Dec 2000 09:31:52 -0500
Subject: Wafer Cross sections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Does anyone have suggestions as to how to polish silicon wafers in cross
section. I have a wafer (~0.5mm thick ) which has been randomly fractured
with about a micron of metal on the surface and want to determine the metal
thickness. We are trying various methods - first choice is by RBS but
presently limited in max. energy so can not penetrate layer.

In my first couple of attempts at polishing (embedded wafer in thermoset)
the silicon breaks quite easily producing a very rough surface. I assume I
am being too aggressive and am trying slower more gentle procedure. Does
anyone have a procedure for polishing cross sections of such brittle material?

Thanks,
Ed



From daemon Tue Dec 5 10:16:16 2000



From: Timothy Schneider :      Timothy.Schneider-at-Mail.TJU.EDU
Date: Tue, 5 Dec 2000 09:47:29 -0500
Subject: SERIAL SECTIONS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


The following comments are for the person who is trying to cut serial
sections with Spurrs. I routinely cut serial sections with Spurrs without
resorting to any kind of glue. Probably the most important thing is how the
block is faced. I cut the trapezoid with a really long (.6 to .9 mm base)
and the top of the trapezoid is almost the same length. What's really
critical here is that those two cuts are parallel.
The two sides of the trapezoid are very short (.1mm). I cut the sections
with a Diatome knife and on a Reichert Ultracut E with a cutting speed of
0.8mm per second and a thickness setting of 80nm. I am doing this in a
small room with a blasting air vent in the ceiling. I have cut up a
cardboard box and stuck it into the ceiling tiles in such a way that the air
is diverted away from the microtome. I can pick up 15 to 25 sections in a
row on a 0.4 X 2mm copper slotted grid coated with just formvar or
formvar/carbon. I have never been any good at making the support films my
self, so I buy the slotted grids precoated from Ted Pella (catalog #s 01706,
01806, or 01816). Before picking up the ribbon, I dip a stick into
chloroform and wave that over the sections, and they expand (or relax,
depending on your point of view). I have a bunch of self locking tweezers
and after picking up the sections, I leave the grid with the sections on it
locked in the tweezers until they dry (if you put a wet slotted grid down on
filter paper it might break the film). Good luck, Tim

Timothy G. Schneider
Director of Electron Microscopy
Department of Pathology
Room 229 Jefferson Hall
Thomas Jefferson University
1020 Locust St.
Philadelphia Pa. 19107
215-503-4798 work
610-613-8170 cellular
timothy.schneider-at-mail.tju.edu




From daemon Tue Dec 5 10:43:18 2000



From: Caroline Schooley :      schooley-at-mcn.org
Date: Mon, 4 Dec 2000 17:06:29 -0800
Subject: Re: tricks for better ribbons?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} Trying to cut serial sections of Spurr for 3D reconstruction. Anyone know
} of any tricks to make the sections stay together in long ribbons? Ellen
} Morgan

Ellen -

I assume that you're talking about EM. not LM. Make sure that the top and
bottom of your block face are REALLY smooth and clean - and as small as is
consistent with the reconstruction. Viewed thru the microtome binocular,
they should look glassy, not like sandpaper. Change your trimming tool
(glass knife? Weck razor blade?) often.

Caroline


Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.msa.microscopy.com/ProjectMicro/PMHomePage.html
Intertidal invertebrates: http://www.fortbragg.k12.ca.us/AG/marinelab.html





From daemon Tue Dec 5 11:22:55 2000



From: chris smith (IACR-RES) :      chris.smith-at-bbsrc.ac.uk
Date: Tue, 5 Dec 2000 17:13:27 -0000
Subject: HP LJ IIIp - EDAX

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All,

Has anyone used a HP Laserjet IIIp with an EDAX PV9900, or know what drivers
are needed, or where we can acquire them?

We are trying to use the IIIp because it has serial and parallel connections
needed by the EDAX and by the Philips XL40 SEM with which we use it and we
cant afford to buy a new one.

Reply in confidence to chris.smith-at-bbsrc.ac.uk if you don't want to clog up
the list.

Many thanks, Chris, Plant Path., IACR-Rothamsted, UK.



From daemon Tue Dec 5 12:46:27 2000



From: Edward Hirsch :      edhirsch-at-att.net
Date: Tue, 5 Dec 2000 13:38:12 -0600
Subject: Wafer Cross sections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ed,
I represent Allied High Tech Products. We have detailed procedures for
preparing semiconductor device cross-sections. We also offer all the
necessary products to accomplish it. I would be happy to speak to you and
provide you with detailed procedures on how to prepare these samples. Since
it can be quite detailed it would be best to do this offline. Please
provide me with your phone number and I will call you.

Sincerely,

Ed

*************************************************
Edward A. Hirsch
Product Application Specialist
Allied High Tech Products
2376 East Pacifica Place
Rancho Dominguez, CA 90220
ph: (919) 846-9628
vm:(800)675-1118 x245
fx: (310)762-6808
http://www.alliedhightech.com

Equipment and Consumables for Metallurgical Sample Preparation
*************************************************



-----Original Message-----
} From: Ed Kurz [mailto:ekurz-at-mail.ims.uconn.edu]
Sent: Tuesday, December 05, 2000 8:32 AM
To: MICROSCOPY BB


Does anyone have suggestions as to how to polish silicon wafers in cross
section. I have a wafer (~0.5mm thick ) which has been randomly fractured
with about a micron of metal on the surface and want to determine the metal
thickness. We are trying various methods - first choice is by RBS but
presently limited in max. energy so can not penetrate layer.

In my first couple of attempts at polishing (embedded wafer in thermoset)
the silicon breaks quite easily producing a very rough surface. I assume I
am being too aggressive and am trying slower more gentle procedure. Does
anyone have a procedure for polishing cross sections of such brittle
material?

Thanks,
Ed




From daemon Tue Dec 5 13:57:36 2000



From: Shalvoy, Richard B **CHES :      RBShalvoy-at-archchemicals.com
Date: Tue, 5 Dec 2000 13:54:19 -0600
Subject: Value of used EDS detector?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have a two year old PGT Prism EDS detector/preamp/dewar assembly which we
are thinking of selling. But I don't have much of an idea of the value of
such a device.

Any thoughts?

Anyone interested?

Richard Shalvoy
Arch Chemicals
Cheshire, CT
203-271-4394



From daemon Tue Dec 5 13:57:41 2000



From: Nafisa Ghori :      nghori-at-stanford.edu
Date: Tue, 05 Dec 2000 11:52:02 -0800
Subject: Negative stain

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear all ,
Does any body use Ammonium Molybdate for Negative staining and also by this
chemical visualize structure better then Uranyl acetate.



From daemon Tue Dec 5 19:25:57 2000



From: Koh YinHsian-CYK006 :      Yinhsian.Koh-at-Motorola.com
Date: Wed, 6 Dec 2000 09:13:53 +0800
Subject: Polymers TEM/SEM position in Texas

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Sorry Jeff,

This is not a job application. Would like to seek your advice on how to analyse a polymer samples using TEM. We have tried on Poly-carbonate injection molded lense with hard coating. The purpose is to see the interface bonding of the hard coat on the lense. Unfortunately, when we microtome the samples, the sample curve up and we cannot manage to see anything. Would appreciate if you can share your experience on the sample preparation with us.

Thanks in advance of your help.

Regards,
YH Koh
(MQE Engineer)
Motorola CGISS Penang,
Bayan Lepas FIZ, Phase 3,
11900 Penang, Malaysia.
Tel : 60-4-8504089
Fax: 60-4-6124903


-----Original Message-----
} From: charles j day [mailto:wa5ekh-at-juno.com]
Sent: Sunday, December 03, 2000 12:50 PM
To: Microscopy-at-sparc5.microscopy.com


There may be a position opening up in the Dallas/Ft. Worth Area for an
experienced microscopist with polymers-TEM experience. If you might be
interested please send me a resume.
Jeff Day/wa5ekh-at-juno.com
________________________________________________________________
GET INTERNET ACCESS FROM JUNO!
Juno offers FREE or PREMIUM Internet access for less!
Join Juno today! For your FREE software, visit:
http://dl.www.juno.com/get/tagj.



From daemon Tue Dec 5 19:54:04 2000



From: Bill Miller :      microbill-at-mohawk.net
Date: Tue, 5 Dec 2000 19:51:05 -0600
Subject: Fw: Wavefront Reconstruction

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


forwarded from the imaging news group

"james patrick birrell" {jbirrell-at-students.uiuc.edu} wrote in message
news: {Pine.GSO.4.10.10012041635310.22505-100000-at-ux12.cso.uiuc.edu} ...
} I was wondering if anybody would know where I could obtain software to
} facilitate the acquisition/analysis of images to be used to perform
} wavefront reconstruction using images from a TEM. Specifically, I'm
} looking for any software compatable with a JEOL 4000ex microscope with a
} Gatan CCD camera.
} Thanks,
} James Birrell





From daemon Tue Dec 5 21:45:09 2000



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Tue, 05 Dec 2000 19:43:40 -0800
Subject: Re: Negative stain

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ammonium molybdate (AM) is suitable in the cases when uranyl acetate (UA)
is bad (mostly if pH is a critical factor, UA pH = 5-5.5). In most cases AM
gives less contrast than UA. AM gives you "pure" negative staining because
do not chemically interfere with most biological samples. UA from another
hand sometimes gives you mixed contrast, positive
(DNA/RNA)&negative(proteins). For instance, ribosomes and many viruses
shown mixed staining with UA. It's not bad because it may even enhance
some structural details, but you have to remember about that. My own
experience indicated that in most cases UA or other uranium salts
(U-formiate or oxalate) after conditions adjusting (concentration, time,
temperature, freshness, support film) has shown perfect results. If you
have a problem with stain spreading, you may try different support films
(carbon, formvar, parlodion). Very good results you may obtain using
"double carbon" technique - this may solv even very worse cases. I also
has have a good results with poly-lysine treatment of the grids prior
sample adsorption. Personally, I don't like the glow-discharge. But it
works in some way.

Sergey.

At 11:52 AM 12/5/00 -0800, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu






From daemon Tue Dec 5 21:47:45 2000



From: Vitaly Feingold :      vitalylazar-at-worldnet.att.net
Date: Tuesday, December 05, 2000 2:11 PM
Subject: HP LJ IIIp - EDAX

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Chris,

EDAX has the HP laser printer driver for old DEC based systems. Call EDAX,
or www.edax.com

Vitaly Feingold
Scientific Instruments and Applications
2773 Heath Lane, Duluth GA 30096
(770)232-7785 ph.
(770)232-1791 fax

This message is made of 100% recycled electrons.
-----Original Message-----
} From: chris smith (IACR-RES) {chris.smith-at-bbsrc.ac.uk}
To: 'Microscopy-at-MSA.Microscopy.Com' {Microscopy-at-sparc5.microscopy.com}





From daemon Wed Dec 6 02:14:07 2000



From: Bart De Pauw :      Bart.DePauw-at-rug.ac.be
Date: Wed, 06 Dec 2000 09:10:17 +0100
Subject: SEM - Choice of buffer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello listers,

We use cacodylate buffer for our SEM samples. Is HEPES or PIPES buffer
an alternative for the toxic cacodylate ? Our samples are mainly animal
tissue.


Bart De Pauw
Ghent University
Faculty of Veterinary Medicine
Department Morphology
Belgium




From daemon Wed Dec 6 03:04:03 2000



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Wed, 6 Dec 2000 09:01:12 +0000 (GMT Standard Time)
Subject: Re: SERIAL SECTIONS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I found your post interesting. How do you make your edges
parallel? Do you use a razor blade?

Dave



On Tue, 5 Dec 2000 09:47:29 -0500 Timothy Schneider
{Timothy.Schneider-at-Mail.TJU.EDU} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} The following comments are for the person who is trying to cut serial
} sections with Spurrs. I routinely cut serial sections with Spurrs without
} resorting to any kind of glue. Probably the most important thing is how the
} block is faced. I cut the trapezoid with a really long (.6 to .9 mm base)
} and the top of the trapezoid is almost the same length. What's really
} critical here is that those two cuts are parallel.
} The two sides of the trapezoid are very short (.1mm). I cut the sections
} with a Diatome knife and on a Reichert Ultracut E with a cutting speed of
} 0.8mm per second and a thickness setting of 80nm. I am doing this in a
} small room with a blasting air vent in the ceiling. I have cut up a
} cardboard box and stuck it into the ceiling tiles in such a way that the air
} is diverted away from the microtome. I can pick up 15 to 25 sections in a
} row on a 0.4 X 2mm copper slotted grid coated with just formvar or
} formvar/carbon. I have never been any good at making the support films my
} self, so I buy the slotted grids precoated from Ted Pella (catalog #s 01706,
} 01806, or 01816). Before picking up the ribbon, I dip a stick into
} chloroform and wave that over the sections, and they expand (or relax,
} depending on your point of view). I have a bunch of self locking tweezers
} and after picking up the sections, I leave the grid with the sections on it
} locked in the tweezers until they dry (if you put a wet slotted grid down on
} filter paper it might break the film). Good luck, Tim
}
} Timothy G. Schneider
} Director of Electron Microscopy
} Department of Pathology
} Room 229 Jefferson Hall
} Thomas Jefferson University
} 1020 Locust St.
} Philadelphia Pa. 19107
} 215-503-4798 work
} 610-613-8170 cellular
} timothy.schneider-at-mail.tju.edu
}
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"




From daemon Wed Dec 6 03:43:39 2000



From: Robert H. Olley :      r.h.olley-at-reading.ac.uk
Date: Wed, 6 Dec 2000 09:40:12 +0000 (GMT Standard Time)
Subject: RE: Polymers TEM/SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



On Wed, 6 Dec 2000, Koh YinHsian-CYK006 wrote:

{ { Would like to seek your advice on how to analyse a polymer sample using
TEM. We have tried on Poly-carbonate injection molded lens with hard
coating. The purpose is to see the interface bonding of the hard coat on
the lens. Unfortunately, when we microtome the samples, the sample curves
up and we cannot manage to see anything. Would appreciate if you can share
your experience on the sample preparation with us. } }

We have looked at surface layers in polymer samples by cutting open a
surface, then etching and looking, NOT at a cut section, but at the
cross-sectional view of the remaining surface, either by SEM or by
replication/TEM. Usually we etch our specimens, so before cutting open, a
protective layer is applied over the outer surface to prevent this being
etched downwards. Mainly we have looked at polyethylene or polypropylene
specimens, using our permanganic etching technique, and different reagents
would be needed for polycarbonate. However, it might be possible to
replicate the cut surface directly and get useful information.

+------------------------------------------------------------------------+
| Robert H.Olley Phone: |
| J.J.Thomson Physical Laboratory {direct line +44 (0) 118 9318572 |
| University of Reading {University internal extension 7867 |
| Whiteknights Fax +44 (0) 118 9750203 |
| Reading RG6 6AF Email: R.H.Olley-at-reading.ac.uk |
| England URL: http://www.reading.ac.uk/~spsolley |
+------------------------------------------------------------------------+





From daemon Wed Dec 6 08:39:14 2000



From: Timothy Schneider :      Timothy.Schneider-at-Mail.TJU.EDU
Date: Wed, 6 Dec 2000 09:24:49 -0500
Subject: MORE SERIAL SECTIONS

Contents Retrieved from Microscopy Listserver Archives
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The question was: how do I make the top and bottom edges parallel? I do it
by eye. I use Gem single edge industrial blades from Ted Pella Cat# 121-1
(I'm sure these are available from many sources). One important point is
that I spray them with 70% ETOH before using and wipe them off with a
Kimwipe EXL. A word about the way I described the trapezoid face: it
doesn't look like a trapezoid. What it looks like is a very thin shiny line
(heck, I'm talking about putting 15 to 25 of these guys on a 2mm slot, you
do the math). More good luck, Tim

Timothy G. Schneider
Director of Electron Microscopy
Department of Pathology
Room 229 Jefferson Hall
Thomas Jefferson University
1020 Locust St.
Philadelphia Pa. 19107
215-503-4798 work
610-613-8170 cellular
timothy.schneider-at-mail.tju.edu




From daemon Wed Dec 6 08:58:56 2000



From: Frank Thomas :      thomasf-at-AGC.BIO.NS.CA
Date: Wed, 6 Dec 2000 10:54:31 -0400
Subject: subscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html







From daemon Wed Dec 6 09:23:04 2000



From: carla_aiwohi-at-usgs.gov
Date: Wed, 6 Dec 2000 07:19:13 -0800
Subject: thanks-SEM conductive adhesive help

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thanks to all who responded to my question regarding conductive adhesives
for salmon skin.
I have ordered many of the products that were suggested and am looking
forward to success (finally) :)

Thanks again and have a great holiday season!


Carla Aiwohi
Western Fisheries Research Center
Seattle, WA




From daemon Wed Dec 6 09:55:48 2000



From: Tom Phillips :      PhillipsT-at-missouri.edu
Date: Wed, 6 Dec 2000 09:34:38 -0600
Subject: Re: SEM - Choice of buffer

Contents Retrieved from Microscopy Listserver Archives
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We routinely use HEPES for our fixations with paraformaldehyde,
glutaraldehyde and osmium. We see little or no difference from
classical cacodylate buffers. I chose HEPES a long time ago and in
retrospect, PIPES might have been a slightly better choice since
HEPES has a pKa of 7.5 and PIPES has a pKa of 6.76. Since one
generally uses aldehyde buffers at pH 7.0 to 7.4, either would seem
appropriate but since I read that during fixation, H+ ions are
generated and the solution tends to acidify, then having a buffer on
the high side of the pKa would be better since you would have greater
buffering power. Despite this "improved" logic, I have remained
loyal to HEPES and never had a problem. Good luck.



} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

--
Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)



From daemon Wed Dec 6 10:49:13 2000



From: Tamara Howard :      howard-at-cshl.org
Date: Wed, 6 Dec 2000 11:44:32 -0500 (EST)
Subject: Gurr buffer recipe?

Contents Retrieved from Microscopy Listserver Archives
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Would anyone happen to have the actual recipe for Gurr's buffer? I think
it is some phosphate formulation - we've been hunting the recipe and have
found some refs, but will have to interlibrary the journals/books and we
thought we'd give the 'net a try, first.

I know we can buy the pre-made buffer, but we need such a tiny amount that
we'd rather make it from scratch (plus we want to know what it is!).

Thanks!

Tamara Howard
Cold Spring Harbor Laboratory, NY




From daemon Wed Dec 6 11:13:57 2000



From: Malcolm Haswell :      malcolm.haswell-at-sunderland.ac.uk
Date: Wed, 06 Dec 2000 17:20:07 +0000
Subject: Re: SERIAL SECTIONS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Timothy

I would be a bit concerned about your use of chloroform to flatten sections,
especially in a small microtome room. We have used heat pens for the last decade
or so, because apparently the chloroform we use these days is a lot more toxic
than it used to be (as well as formaldehyde, glutaraldehyde, carbon
tetrachloride etc).

Malcolm Haswell
e.m. unit
School of Sciences
University of Sunderland
UK

Timothy Schneider wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} The following comments are for the person who is trying to cut serial
} sections with Spurrs. I routinely cut serial sections with Spurrs without
} resorting to any kind of glue. Probably the most important thing is how the
} block is faced. I cut the trapezoid with a really long (.6 to .9 mm base)
} and the top of the trapezoid is almost the same length. What's really
} critical here is that those two cuts are parallel.
} The two sides of the trapezoid are very short (.1mm). I cut the sections
} with a Diatome knife and on a Reichert Ultracut E with a cutting speed of
} 0.8mm per second and a thickness setting of 80nm. I am doing this in a
} small room with a blasting air vent in the ceiling. I have cut up a
} cardboard box and stuck it into the ceiling tiles in such a way that the air
} is diverted away from the microtome. I can pick up 15 to 25 sections in a
} row on a 0.4 X 2mm copper slotted grid coated with just formvar or
} formvar/carbon. I have never been any good at making the support films my
} self, so I buy the slotted grids precoated from Ted Pella (catalog #s 01706,
} 01806, or 01816). Before picking up the ribbon, I dip a stick into
} chloroform and wave that over the sections, and they expand (or relax,
} depending on your point of view). I have a bunch of self locking tweezers
} and after picking up the sections, I leave the grid with the sections on it
} locked in the tweezers until they dry (if you put a wet slotted grid down on
} filter paper it might break the film). Good luck, Tim
}
} Timothy G. Schneider
} Director of Electron Microscopy
} Department of Pathology
} Room 229 Jefferson Hall
} Thomas Jefferson University
} 1020 Locust St.
} Philadelphia Pa. 19107
} 215-503-4798 work
} 610-613-8170 cellular
} timothy.schneider-at-mail.tju.edu




From daemon Wed Dec 6 11:44:29 2000



From: m.andersson-at-t-online.de (Maike Andersson)
Date: Wed, 6 Dec 2000 18:48:01 +0100
Subject: Need help on staining semithin sections embedded in LR-white

Contents Retrieved from Microscopy Listserver Archives
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LM - Need help on staining semithin sections embedded in LR-white

I am doing anatomical studies on the seed coat and currently use toluidine
blue
with sodium hypochlorite prestaining and iodine/potassium iodite
poststaining, described by
Gutmann, M. : Improved staining procedures for photografic documentation of
phenolic deposits in semithin sections of plant tissue. Journal of
Microscopy 179:277-281 (1995)
Can anyone suggest a better stain, specially one that is suitable for
staining lignin ?




From daemon Wed Dec 6 13:27:07 2000



From: Barbara Plowman :      Bplowman-at-sfmail.dental.uop.edu
Date: Wed, 06 Dec 2000 11:19:42 -0800
Subject: Ammonium molybdate

Contents Retrieved from Microscopy Listserver Archives
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I have used 1% ammonium molybdate, pH7.3 -at-300milliosmoles for negative staining of liposomes. It worked better than UA.

Barbara L. Plowman
University of the Pacific
School of Dentistry
San Francisco, CA
phone: 415-929-6692
email: Bplowman-at-sfuop.edu




From daemon Wed Dec 6 13:27:12 2000



From: Zhenquan Liu :      zhenquan.liu-at-asu.edu
Date: Wed, 06 Dec 2000 12:21:15 -0700
Subject: Re: Wafer Cross sections

Contents Retrieved from Microscopy Listserver Archives
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Hi, Ed,

I think you may try the following way:

1. Apply a thin layer of M-bond on the surfaces of two pieces of your wafer
and put these two surfaces together.
2. Use a clip to press these pieces of wafers as close as possible (not to
break them).
3. Leave the sample to try (24 hours in air? or 1 hour at 120 C degree on a
hot plate).

This is a good way to protect the thin "film" (your metal features) I
think. The smaller the gap between these two pieces, the better. In this
way you can get two cross section surfaces. Even after step 3, you may
apply another thin layer of M-bond on the outside surfaces of the sample,
so that you may get 4 surfaces for examination.

4 Embed the sample in a suitable epoxy as you did before, and grinding and
polishing the cross sections carefully from coarse to fine.

When you observe the cross sections, it is easy to see what is the M-bond
and what is the other thing you want to see.

Just a suggestion.

Good luck!

Zhenquan Liu


I think you may try the following way:

1. Apply a thin layer of M-bond on the surfaces of two pieces of your wafer
and put these two surfaces together.
2. Use a clip to press these pieces of wafers as close as possible (not to
break them).
3. Leave the sample to try (24 hours in air? or 1 hour at 120 C degree on a
hot plate).

This is a good way to protect the thin "film" (your metal features) I
think. The smaller the gap between these two pieces, the better. In this
way you can get two cross section surfaces. Even after step 3, you may
apply another thin layer of M-bond on the outside surfaces of the sample,
so that you may get 4 surfaces for examination.

4 Embed the sample in a suitable epoxy as you did befoe, and grinding and
polishing the cross sections carefully from coarse to fine.

When you observe the cross sections, it is easy to see what is the M-bond
and what is the other thing you want to see.

Just a suggestion.

Good luck!

Zhenquan Liu


=================

Does anyone have suggestions as to how to polish silicon wafers in cross
section. I have a wafer (~0.5mm thick ) which has been randomly fractured
with about a micron of metal on the surface and want to determine the metal
thickness. We are trying various methods - first choice is by RBS but
presently limited in max. energy so can not penetrate layer.

In my first couple of attempts at polishing (embedded wafer in thermoset)
the silicon breaks quite easily producing a very rough surface. I assume I
am being too aggressive and am trying slower more gentle procedure. Does
anyone have a procedure for polishing cross sections of such brittle material?

Thanks,
Ed

------------------------------------
Zhenquan Liu (Dr.)
Arizona State University
Room 146A, CSSS
Tempe, AZ 85287
Tel (480) 965 4544 (o)
(480) 775 7428 (h)
Fax (480) 965 9004 (o)
Email zhenquan.liu-at-asu.edu
------------------------------------





From daemon Wed Dec 6 14:05:17 2000



From: Malcolm Haswell :      malcolm.haswell-at-sunderland.ac.uk
Date: Wed, 06 Dec 2000 16:34:41 +0000
Subject: Re: SEM - Choice of buffer

Contents Retrieved from Microscopy Listserver Archives
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Bart

I have used PIPES as a substitute for cacodylate in most routine procedures
simply because it has none of the precipitation problems of phosphate
buffers. I understand that there may be more extraction with cacodylate in
TEM so I would have thought that it may be a better buffer for SEM (less
chance of shrinkage?). I would assume that HEPES was similar but have no
experience with it.

These are my own feelings and I have seen no scientific evidence to support
this - has anyone else?


Malcolm Haswell
e.m. unit
School of Sciences
University of Sunderland
UK

Bart De Pauw wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Hello listers,
}
} We use cacodylate buffer for our SEM samples. Is HEPES or PIPES buffer
} an alternative for the toxic cacodylate ? Our samples are mainly animal
} tissue.
}
} Bart De Pauw
} Ghent University
} Faculty of Veterinary Medicine
} Department Morphology
} Belgium




From daemon Wed Dec 6 17:20:39 2000



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Wed, 06 Dec 2000 15:16:20 -0800
Subject: Fwd: Re: MORE SERIAL SECTIONS

Contents Retrieved from Microscopy Listserver Archives
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} To: Timothy.Schneider-at-Mail.TJU.EDU
} From: Sergey Ryazantsev {sryazant-at-ucla.edu}
} Subject: Re: MORE SERIAL SECTIONS
}
} If you are not limited by particular area in your sample, you may do the
} trick, which warranty the parallelness of the edges. This trick did show
} to me Prof. Borovyagim many years ago. The trick is: you do not form a
} trapezoid, instead you make two cuts by razor blade, which owerlapping, so
} you will have "/\"-shape without flat top. The top should be formed by
} two overlapped cuts. There is no flat space on the top, which we usually
} called "trapezoid". This two cuts will form a "line" which will be
} paralle to the knife edge later. You have to form side fases of the
} piramid as well. Finall, you will have something looks pretty like
} triangle prizm. Not perfect prizm, of coarse. When you start cutting,
} you have to carefully cut avay the top of the "prizm" to form
} trapezoid. The final shape of the trapezoid will depends from orientation
} of your cuts, but owerlapped cuts will form perfectly parallel sides.
}
} Another choice I friequently use: to use glass knife and trim on the
} goniometer-head when you may tilt and rotate the sample. Make one face,
} rotate 180 deg and make second face.
}
} At 09:24 AM 12/6/00 -0500, you wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu






From daemon Wed Dec 6 17:20:40 2000



From: Phillip Rutledge :      prutledge-at-msa-stoneville.ars.usda.gov
Date: Thu, 06 Apr 2000 14:37:21 -0500
Subject: LM--TISSUE PROCESSORS

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I am currently in the process of setting up a histology/microbiology/EM facility. Does anyone have comments or suggestions on a good rotary tissue processor for light microscopy? My main concerns are cost, service availability, ease in operation(changing of fluids, etc.) and reliability. I may process 10 - 60 specimens a month, more as the lab is established. I appreciate any info.

Thanks,

Phil Rutledge
prutledge-at-ars.usda.gov



From daemon Wed Dec 6 17:20:40 2000



From: Phillip Rutledge :      prutledge-at-msa-stoneville.ars.usda.gov
Date: Thu, 13 Apr 2000 06:38:28 -0500
Subject: TISSUE PROCESSORS

Contents Retrieved from Microscopy Listserver Archives
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I'm currently in the process of setting up a Histology/Microbiology/EM Lab. Having been away from histology for almost 30 years (been doing EM), I've gotten away from instrumentation associated with histology. Does anyone have a good recommendation for a rotary tissue processor? I've got info on Shandon and Tissue-Tek. Are there others out there?
Thanks for any info.

Phil
PRUTLEDGE-at-ARS.USDA.GOV





From daemon Wed Dec 6 17:22:05 2000



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Wed, 06 Dec 2000 15:20:52 -0800
Subject: Fwd: Re: MORE SERIAL SECTIONS

Contents Retrieved from Microscopy Listserver Archives
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} Date: Wed, 06 Dec 2000 15:16:31 -0800
} To: Timothy.Schneider-at-Mail.TJU.EDU
} From: Sergey Ryazantsev {sryazant-at-ucla.edu}
} Subject: Re: MORE SERIAL SECTIONS
}
} If you are not limited by particular area in your sample, you may do the
} trick, which warranty the parallelness of the edges. This trick did show
} to me Prof. Borovyagin many years ago. The trick is: you do not form a
} trapezoid, instead you make two cuts by razor blade, which overlapping, so
} you will have "/\"-shape without flat top. The top should be formed by
} two overlapped cuts without "free" space between. There is no flat space
} on the top, which we usually called "trapezoid". This two cuts will form
} a "line" which will be parallel to the knife edge later. You have to
} form side faces of the pyramid as well. Finally, you will have something
} looks pretty like triangle prism. Not perfect prism, of coarse. When you
} start cutting, you have to carefully cut away the top of the "prism" to
} form trapezoid. The final shape of the trapezoid will depends from
} orientation of your cuts, but overlapped cuts will form perfectly parallel
} sides. This technique works if sample will permit.
}
} Another choice I frequently use: to use glass knife and trim on the
} goniometer-head when you may tilt and rotate the sample. Make one face,
} rotate 180 deg and make second face. If I have enough time, I prefer this
} way, almost gives me the perfect pyramid.
}
} Sergey
}
} At 09:24 AM 12/6/00 -0500, you wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu






From daemon Wed Dec 6 17:22:48 2000



From: Phillip Rutledge :      prutledge-at-msa-stoneville.ars.usda.gov
Date: Mon, 17 Apr 2000 10:27:05 -0500
Subject: LM - Tissue Processors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I'm currently in the process of setting up a Histology/Microbiology/EM facility. Being out of Histology for almost 30 years (been doing EM) I'm not sure of the best type of tissue processor to buy. I won't be doing that many specimens, so I'm leaning towards a rotary tissue processor. I have info on Shandon and Tissue-Tek. Are there others out there? Anyone having opinions on the 2 mentioned, I would appreciate any comments. I am looking for ease of use (changing solutions, fume safeguards), reliability on service and cost effectiveness. Thanks.

Peace be with you,
Phil Rutledge (410)778-4136, 2120
prutledge-at-ars.usda.gov



From daemon Wed Dec 6 17:25:25 2000



From: Dusevich, Vladimir :      DusevichV-at-umkc.edu
Date: Wed, 6 Dec 2000 17:21:51 -0600
Subject: RE: Wafer Cross sections

Contents Retrieved from Microscopy Listserver Archives
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I usually glue (with superglue or thin epoxy) 2-4 wafers together, metal coated
surfaces facing each other. Then embed them or mount in microwise, cut with low
speed diamond saw and polish, preferably with diamond. If you do not need nice
pictures, just measurements, do not use fine polish, you will more or less ruin
edges with it.

Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
3127 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

Phone: (816) 235-2072
Fax: (816) 235-5524
Web: http://www.umkc.edu/dentistry/microscopy

} -----Original Message-----
} From: Ed Kurz [mailto:ekurz-at-mail.ims.uconn.edu]
} Sent: Tuesday, December 05, 2000 8:32 AM
} To: MICROSCOPY BB
} Subject: Wafer Cross sections
}
}
} --------------------------------------------------------------
} ----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} ---------.
}
}
} Does anyone have suggestions as to how to polish silicon
} wafers in cross
} section. I have a wafer (~0.5mm thick ) which has been
} randomly fractured
} with about a micron of metal on the surface and want to
} determine the metal
} thickness. We are trying various methods - first choice is by RBS but
} presently limited in max. energy so can not penetrate layer.
}
} In my first couple of attempts at polishing (embedded wafer
} in thermoset)
} the silicon breaks quite easily producing a very rough
} surface. I assume I
} am being too aggressive and am trying slower more gentle
} procedure. Does
} anyone have a procedure for polishing cross sections of such
} brittle material?
}
} Thanks,
} Ed
}



From daemon Thu Dec 7 00:18:20 2000



From: J. A. Kiernan :      jkiernan-at-julian.uwo.ca
Date: Thu, 7 Dec 2000 01:03:27 -0500 (EST)
Subject: Re: Gurr buffer recipe?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On Wed, 6 Dec 2000, Tamara Howard wrote:

} Would anyone happen to have the actual recipe for Gurr's buffer? I think
} it is some phosphate formulation ...
} I know we can buy the pre-made buffer, but we need such a tiny amount that
} we'd rather make it from scratch (plus we want to know what it is!).

Quite right too! (For making and wanting to know)
I haven't heard of it so can't tell you the answer, BUT:

Does it matter what the exact formulation is? It is unlikely
that either of the late Gurr Bros (Edward, and George T.) invented
an original buffer. They were vendors of stains in Britain, about
whom some older HistoNetters may well have entertaining anecdotes.

If a buffer does its job of stabilizing the pH at the correct
value, and doesn't contain anything that would react with the
other ingredients of the mixture, it shouldn't matter what you
use. Phosphate buffers make a precipitate with many metal salts,
including those of Ca, Co, Cu, Fe, Mg, and others that have
insoluble phosphates. All the techniques books published since
about 1955 have sets of tables for making buffers to cover a
wide range of pH.

John A. Kiernan,
Department of Anatomy & Cell Biology,
The University of Western Ontario,
LONDON, Canada N6A 5C1





From daemon Thu Dec 7 00:22:26 2000



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Wed, 06 Dec 2000 22:18:27 -0800
Subject: Re: Negative stain

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Debby

Ted Pella Poly-L-Lysin 0.1% w/v aqueous solution, Cat # 18026
Float your grid with film on small drop of the solution for 1 min and than
remove excess of the liquid by filter paper and put on the drop of water
for 5-10 sec, blot it and immediately move it on the drop with your sample.
Temperature - as necessary for your sample. It also helps to adsorb tuff
samples. Surprisingly, it seems to me that it does not affect background.

Concerning "double-carbon" technique. It is more tricky. The idea is to
sandwich your sample between two carbon films just before you finish the
staining. I prefer the following technique. The approx. 1 ml plastic cap
(have no idea what is that, you may use any suitable container) 0.7 cm
diameter I filled with 0.5-1% aqueous UA in the way to make flat meniscus,
so it will reflect the light. Than I will cut 3x3 mm piece of mica with
carbon film and float carbon on the UA solution. Reflecting light will
makes it visible under some particular angle (you have to practice a little
bit). So, you have the plastic cap with UA and floated piece of carbon film
on it and you can see carbon easily. Than you will adsorb sample on the
regular EM grid with carbon. Than you have to move your grid into the cap
with stain (avoid contact to the floated carbon, soak grid into the cap
away from carbon). Looking through the carbon film, still floated on the
UA, move the grid just under the film. So, you will see the grid through
floated film. Than using one smooth movement you have to move grid
vertically up picking up the carbon film. So, you will put grid just under
the film (deep under the surface) and than move grid vertically up. The
film will holds over the grid with drop of the stain solution. The last
most important step - to remove excesses of staining solution: fix grid
securely in the tweezer by rubber O-ring (or use "reverse-action" tweezer),
small triangle shape piece of filter paper (I am using Whatmann 1M paper)
insert between tweezer's "legs" (sorry, guys, I have no idea how it is
called) as closer to the grid as possible. Filter paper chip should
contact with excess of liquid holds by capillary force between the
tweezer's "legs". Keep tweezer with grid and filter paper until grid will
dry completely (at least 5 min). Enjoy the perfect spreading of the
stain! There is a number of disadvantages in this method:
-"double-carbon" will decrease the resolution;
-sandwich will slightly flat the particles, expect to see 10-15% bigger
size because of that.

Advantages:
-sandwich will protect your sample from radiation damage;
-such samples usually are more stable from all points of view;
-because of thick carbon, you may use 400 mesh naked EM grids without
"holey" support film (I still prefer to use the grids with my home-made
"holey" film).

PTA vs AM. In my point of view, PTA does not have any serious advantages
over the AM. Personally, I prefer AM. In some particular cases PTA may
work better, I guess. If there is no chance to use UA, I would try
U-formiate first and oxalate - next. U-formiate (UF) has pH around 6 or
even higher (I don't remember the exact number). It gives you a very nice
fine staining (I love it better than UA). The disadvantage of this stain -
it is not stable. You have to prepare fresh solution every time. It is
light-sensitive also (general room illumination is OK for 20-30 min, no
direct light, please!). You could store it in the dark in the ice for
couple of hours. U-oxalate (UO) is less stable than UF, you have to use it
immediately. The beauty of UO - you may use it at pH 7. People's
unsuccess with UF or UO mostly related to the storage problem, I
believe. Most people don't understand how unstable those substances
are. By the way, UA is light-sensitive also.

My staining conditions for all stains are: +4oC (if sample permits),
adsorption time - 1-2 min; staining 0.5-1% aqueous solution - 1-2 min. I
find, that it is better to adjust sample concentration rather than to play
with adsorption time. Extended staining time will not dramatically improve
the staining but may harm the sample's integrity (it depends, for IgG
staining I am using 4 min 1% UA). For UA staining I also prefer to use my
favorite "EM" buffer based on ammonium acetate with additives if necessary
(50-100 mM ammonium acetate, pH 7.8 in simplest case). It is highly
compatible with UA and gives good stain's distribution.

I am sorry if my description is not clear. Please, feel free to call me if
any question. It's much simpler to do than read.

God luck
Sergey



At 11:08 AM 12/6/00 -0500, you wrote:
} Sergey,
} Would you mind giving me the details of your treatment of grids with
} poly-lysine and the double-carbon technique that you refer to in your
} E-mail. I usually go to PTA if PH is a problem. How do you compare PTA
} with AM? I am always looking for new methods to deal with some of the
} negative stain. problems.
} Thanks very much,
} Debby
}
} Debby Sherman Phone: 765-494-6666
} Life Science Microscopy Facility FAX: 765-494-5896
} Purdue University E-mail:
} dsherman-at-purdue.edu
} 1057 Whistler Building
} West Lafayette, IN 47907-1057
}
}
} On Tuesday, December 5, 2000 10:43 PM, Sergey Ryazantsev
} {sryazant-at-ucla.edu} wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Ammonium molybdate (AM) is suitable in the cases when uranyl acetate (UA)
} } is bad (mostly if pH is a critical factor, UA pH = 5-5.5). In most cases AM
} } gives less contrast than UA. AM gives you "pure" negative staining because
} } do not chemically interfere with most biological samples. UA from another
} } hand sometimes gives you mixed contrast, positive
} } (DNA/RNA)&negative(proteins). For instance, ribosomes and many viruses
} } shown mixed staining with UA. It's not bad because it may even enhance
} } some structural details, but you have to remember about that. My own
} } experience indicated that in most cases UA or other uranium salts
} } (U-formiate or oxalate) after conditions adjusting (concentration, time,
} } temperature, freshness, support film) has shown perfect results. If you
} } have a problem with stain spreading, you may try different support films
} } (carbon, formvar, parlodion). Very good results you may obtain using
} } "double carbon" technique - this may solv even very worse cases. I also
} } has have a good results with poly-lysine treatment of the grids prior
} } sample adsorption. Personally, I don't like the glow-discharge. But it
} } works in some way.
} }
} } Sergey.
} }
} } At 11:52 AM 12/5/00 -0800, you wrote:
} } } ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } -----------------------------------------------------------------------.
} } }
} } }
} } } Dear all ,
} } } Does any body use Ammonium Molybdate for Negative staining and also by
} } } this chemical visualize structure better then Uranyl acetate.
} }
} } _____________________________________
} }
} } Sergey Ryazantsev Ph. D.
} } Electron Microscopy
} } UCLA School of Medicine
} } Department of Biological Chemistry
} } Box 951737
} } Los Angeles, CA 90095-1737
} }
} } Phone: (310) 825-1144
} } Pager: (310) 845-0248
} } FAX (departmental): (310) 206-5272
} } mailto:sryazant-at-ucla.edu
} }
} }
} }
} }

_____________________________________

Sergey Ryazantsev Ph. D.
Electron Microscopy
UCLA School of Medicine
Department of Biological Chemistry
Box 951737
Los Angeles, CA 90095-1737

Phone: (310) 825-1144
Pager: (310) 845-0248
FAX (departmental): (310) 206-5272
mailto:sryazant-at-ucla.edu






From daemon Thu Dec 7 01:25:18 2000



From: Bart De Pauw :      Bart.DePauw-at-rug.ac.be
Date: Thu, 07 Dec 2000 08:17:56 +0100
Subject: Re: LM - Tissue Processors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We use the Shandon Citadel 1000. It's a good processor, but there's one disadvantage : there's evaporation of your fluids. There's no fume safeguard on the processor, so you have to put it in a fume hood.

Phillip Rutledge schreef:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} I'm currently in the process of setting up a Histology/Microbiology/EM facility. Being out of Histology for almost 30 years (been doing EM) I'm not sure of the best type of tissue processor to buy. I won't be doing that many specimens, so I'm leaning towards a rotary tissue processor. I have info on Shandon and Tissue-Tek. Are there others out there? Anyone having opinions on the 2 mentioned, I would appreciate any comments. I am looking for ease of use (changing solutions, fume safeguards), reliability on service and cost effectiveness. Thanks.
}
} Peace be with you,
} Phil Rutledge (410)778-4136, 2120
} prutledge-at-ars.usda.gov




From daemon Thu Dec 7 02:37:27 2000



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Thu, 07 Dec 2000 00:32:20 -0800
Subject: Re: Negative stain

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Chris, thanks for your compliments.

I did not publish this technique yet. So, you may refer to our ListServer.
If you need more details - call me.

Sergey.

At 07:52 AM 12/7/00 +0000, you wrote:
} Brilliant, Sergey. Have you published these methods anywhere? If
} so, I would be interested to have the references.
} Best wishes
} Chris
}
} Date sent: Wed, 06 Dec 2000 22:18:27 -0800
} To: Debby Sherman {dsherman-at-purdue.edu}
} From: Sergey Ryazantsev {sryazant-at-ucla.edu}
} Subject: Re: Negative stain
} Copies to: Microscopy-at-sparc5.microscopy.com
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Hello Debby
} }
} } Ted Pella Poly-L-Lysin 0.1% w/v aqueous solution, Cat # 18026
} } Float your grid with film on small drop of the solution for 1 min and than
} } remove excess of the liquid by filter paper and put on the drop of water
} } for 5-10 sec, blot it and immediately move it on the drop with your
} sample.
} } Temperature - as necessary for your sample. It also helps to adsorb tuff
} } samples. Surprisingly, it seems to me that it does not affect background.
} }
} } Concerning "double-carbon" technique. It is more tricky. The idea is to
} } sandwich your sample between two carbon films just before you finish the
} } staining. I prefer the following technique. The approx. 1 ml plastic cap
} } (have no idea what is that, you may use any suitable container) 0.7 cm
} } diameter I filled with 0.5-1% aqueous UA in the way to make flat meniscus,
} } so it will reflect the light. Than I will cut 3x3 mm piece of mica with
} } carbon film and float carbon on the UA solution. Reflecting light will
} } makes it visible under some particular angle (you have to practice a
} little
} } bit). So, you have the plastic cap with UA and floated piece of carbon
} film
} } on it and you can see carbon easily. Than you will adsorb sample on the
} } regular EM grid with carbon. Than you have to move your grid into the cap
} } with stain (avoid contact to the floated carbon, soak grid into the cap
} } away from carbon). Looking through the carbon film, still floated on the
} } UA, move the grid just under the film. So, you will see the grid through
} } floated film. Than using one smooth movement you have to move grid
} } vertically up picking up the carbon film. So, you will put grid just
} under
} } the film (deep under the surface) and than move grid vertically up. The
} } film will holds over the grid with drop of the stain solution. The last
} } most important step - to remove excesses of staining solution: fix grid
} } securely in the tweezer by rubber O-ring (or use "reverse-action"
} tweezer),
} } small triangle shape piece of filter paper (I am using Whatmann 1M paper)
} } insert between tweezer's "legs" (sorry, guys, I have no idea how it is
} } called) as closer to the grid as possible. Filter paper chip should
} } contact with excess of liquid holds by capillary force between the
} } tweezer's "legs". Keep tweezer with grid and filter paper until grid will
} } dry completely (at least 5 min). Enjoy the perfect spreading of the
} } stain! There is a number of disadvantages in this method:
} } -"double-carbon" will decrease the resolution;
} } -sandwich will slightly flat the particles, expect to see 10-15% bigger
} } size because of that.
} }
} } Advantages:
} } -sandwich will protect your sample from radiation damage;
} } -such samples usually are more stable from all points of view;
} } -because of thick carbon, you may use 400 mesh naked EM grids without
} } "holey" support film (I still prefer to use the grids with my home-made
} } "holey" film).
} }
} } PTA vs AM. In my point of view, PTA does not have any serious advantages
} } over the AM. Personally, I prefer AM. In some particular cases PTA may
} } work better, I guess. If there is no chance to use UA, I would try
} } U-formiate first and oxalate - next. U-formiate (UF) has pH around 6 or
} } even higher (I don't remember the exact number). It gives you a very nice
} } fine staining (I love it better than UA). The disadvantage of this
} stain -
} } it is not stable. You have to prepare fresh solution every time. It is
} } light-sensitive also (general room illumination is OK for 20-30 min, no
} } direct light, please!). You could store it in the dark in the ice for
} } couple of hours. U-oxalate (UO) is less stable than UF, you have to
} use it
} } immediately. The beauty of UO - you may use it at pH 7. People's
} } unsuccess with UF or UO mostly related to the storage problem, I
} } believe. Most people don't understand how unstable those substances
} } are. By the way, UA is light-sensitive also.
} }
} } My staining conditions for all stains are: +4oC (if sample permits),
} } adsorption time - 1-2 min; staining 0.5-1% aqueous solution - 1-2 min. I
} } find, that it is better to adjust sample concentration rather than to play
} } with adsorption time. Extended staining time will not dramatically improve
} } the staining but may harm the sample's integrity (it depends, for IgG
} } staining I am using 4 min 1% UA). For UA staining I also prefer to use my
} } favorite "EM" buffer based on ammonium acetate with additives if necessary
} } (50-100 mM ammonium acetate, pH 7.8 in simplest case). It is highly
} } compatible with UA and gives good stain's distribution.
} }
} } I am sorry if my description is not clear. Please, feel free to call
} me if
} } any question. It's much simpler to do than read.
} }
} } God luck
} } Sergey
} }
} }
} }
} } At 11:08 AM 12/6/00 -0500, you wrote:
} } } Sergey,
} } } Would you mind giving me the details of your treatment of grids with
} } } poly-lysine and the double-carbon technique that you refer to in your
} } } E-mail. I usually go to PTA if PH is a problem. How do you compare PTA
} } } with AM? I am always looking for new methods to deal with some of the
} } } negative stain. problems.
} } } Thanks very much,
} } } Debby
} } }
} } } Debby Sherman Phone: 765-494-6666
} } } Life Science Microscopy Facility FAX: 765-494-5896
} } } Purdue University E-mail:
} } } dsherman-at-purdue.edu
} } } 1057 Whistler Building
} } } West Lafayette, IN 47907-1057
} } }
} } }
} } } On Tuesday, December 5, 2000 10:43 PM, Sergey Ryazantsev
} } } {sryazant-at-ucla.edu} wrote:
} } } } ------------------------------------------------------------------------
} } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } } -----------------------------------------------------------------------.
} } } }
} } } }
} } } } Ammonium molybdate (AM) is suitable in the cases when uranyl acetate
} (UA)
} } } } is bad (mostly if pH is a critical factor, UA pH = 5-5.5). In most
} cases AM
} } } } gives less contrast than UA. AM gives you "pure" negative staining
} because
} } } } do not chemically interfere with most biological samples. UA from
} another
} } } } hand sometimes gives you mixed contrast, positive
} } } } (DNA/RNA)&negative(proteins). For instance, ribosomes and many viruses
} } } } shown mixed staining with UA. It's not bad because it may even enhance
} } } } some structural details, but you have to remember about that. My own
} } } } experience indicated that in most cases UA or other uranium salts
} } } } (U-formiate or oxalate) after conditions adjusting (concentration,
} time,
} } } } temperature, freshness, support film) has shown perfect results. If you
} } } } have a problem with stain spreading, you may try different support films
} } } } (carbon, formvar, parlodion). Very good results you may obtain using
} } } } "double carbon" technique - this may solv even very worse cases. I also
} } } } has have a good results with poly-lysine treatment of the grids prior
} } } } sample adsorption. Personally, I don't like the glow-discharge. But it
} } } } works in some way.
} } } }
} } } } Sergey.
} } } }
} } } } At 11:52 AM 12/5/00 -0800, you wrote:
} } } } } --------------------------------------------------------------------
} ----
} } } } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } } } To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} } } } } On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } } } --------------------------------------------------------------------
} ---.
} } } } }
} } } } }
} } } } } Dear all ,
} } } } } Does any body use Ammonium Molybdate for Negative staining and also by
} } } } } this chemical visualize structure better then Uranyl acetate.
} } } }
} } } } _____________________________________
} } } }
} } } } Sergey Ryazantsev Ph. D.
} } } } Electron Microscopy
} } } } UCLA School of Medicine
} } } } Department of Biological Chemistry
} } } } Box 951737
} } } } Los Angeles, CA 90095-1737
} } } }
} } } } Phone: (310) 825-1144
} } } } Pager: (310) 845-0248
} } } } FAX (departmental): (310) 206-5272
} } } } mailto:sryazant-at-ucla.edu
} } } }
} } } }
} } } }
} } } }
} }
} } _____________________________________
} }
} } Sergey Ryazantsev Ph. D.
} } Electron Microscopy
} } UCLA School of Medicine
} } Department of Biological Chemistry
} } Box 951737
} } Los Angeles, CA 90095-1737
} }
} } Phone: (310) 825-1144
} } Pager: (310) 845-0248
} } FAX (departmental): (310) 206-5272
} } mailto:sryazant-at-ucla.edu
} }
} }
} }
} }
}
}
} ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
} Dr Chris Jeffree
} University of Edinburgh
} Biological Sciences EM Facility
} Daniel Rutherford Building
} King's Buildings EDINBURGH EH9 3JH
} Tel: +44 (0) 131 650 5345
} FAX: +44 (0) 131 650 6563
}
} Inveresk Cottage, 26 Carberry Road,
} Inveresk, Musselburgh, Midlothian EH21 8PR, UK
} Tel: +44 (0) 131 665 6062 / Mobile 07710 585 401
} FAX: +44 (0) 131 653 6248
} ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~




From daemon Thu Dec 7 03:02:50 2000



From: Sylke Helbing :      Sylke.Helbing-at-embl-heidelberg.de
Date: Thu, 7 Dec 2000 09:56:18 +0100
Subject: EM - Course on Electron Microscopy, Immunocytochemistry and

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


EMBO Practical Course on Electron Microscopy, Immunocytochemistry and
Stereology for Cell Biology at EMBL, Heidelberg, Germany, Aug 30 - Sep 8,
2001

This is a course about the production of thin sections of biological
material and their use in studying ultrastructure in the context of
molecular cell biology. The course will introduce the techniques of
cryosectioning, rapid freezing methods as an alternative to chemical
fixation, freeze substitution and resin embedding. It will instruct
participants in the sectioning of suitably prepared material and will then
concentrate on the use of these sections for localizing specific molecules
within cells. Ample time will be given to hands on practical work as well
as formal and informal discussion of available techniques for the
localization of subcellular molecules.

Further info: http://www.EMBL-Heidelberg.DE/courses/ElectronMicroscopy01/

****************************
Sylke Helbing
Course and Conference Office
European Molecular Biology Laboratory
Meyerhofstr. 1
D-69117 Heidelberg

Tel: +49-6221-387 106
Fax: +49-6221-387 158
Email: helbing-at-embl-heidelberg.de
****************************





From daemon Thu Dec 7 04:41:47 2000



From: HARRISm-at-esm-semi.co.uk
Date: Thu, 07 Dec 2000 10:31 +0000 (GMT)
Subject: Coating v's Bonding

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Hi ,

RE: Semiconductor back end processes

A general question whether anyone knows where I can obtain information
on the metallisation or backside coating (through evaporation) of
silicon wafers after backgrinding ?
My limited previous metals coating experience has given me the
impression that true metal bonding is the result of the formation of a
intermetallic/segregated region - of both metallic constituents
cooling to form what is basically a weld zone with no discernible
porosity interface .
A mechanical bond on the other hand would simply be the 'knitting' of
rough surfaces .
My interest includes the goal of successful metallisation and how it
is assessed as normal mechanical preparation of a cross section can
tear and smear the face causing difficulty in porosity / interface
evaluation . Is FIB used or are there non destructive methods for
example ?


Thanks

Martyn Harris
ESM LTD
Cardiff Rd, Newport , Gwent
South Wales , UK .
Email : harrism-at-esm-semi.co.uk




From daemon Thu Dec 7 07:20:48 2000



From: Roger Moretz :      rcmoretz-at-excite.com
Date: Thu, 7 Dec 2000 05:14:48 -0800 (PST)
Subject: Re: SERIAL SECTIONS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Malcolm:

I don't think the chloroform has gotten more toxic (unless I forgot about
that fact, having used that technique for more years than I can
remember....). Hopefully, a lot of us have gotten a tiny bit smarter about
exposure to potentially harmful chemicals. Actually, over the years I have
developed sensitivity to a number of chemicals in the EM Lab, and still tend
to be a little careless about exposure to vapors - especially formalin,
glut, osmium, xylene, acetone, EtOH. But I, too, have used the heat pens
for well over 20 years (ever since the first cautery pens became available
thru one of the EM suppliers), and urge individuals who do a lot of
sectioning to switch to the heat pens.

Roger Moretz, Ph.D.
Dept of Toxicology
Boehringer Ingelheim Pharmaceuticals

On Wed, 06 Dec 2000 17:20:07 +0000, Malcolm Haswell wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Timothy
}
} I would be a bit concerned about your use of chloroform to flatten
sections,
} especially in a small microtome room. We have used heat pens for the last
decade
} or so, because apparently the chloroform we use these days is a lot more
toxic
} than it used to be (as well as formaldehyde, glutaraldehyde, carbon
} tetrachloride etc).
}
} Malcolm Haswell
} e.m. unit
} School of Sciences
} University of Sunderland
} UK
}
} Timothy Schneider wrote:
}
} }
------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
} } On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} }
-----------------------------------------------------------------------.
} }
} } The following comments are for the person who is trying to cut serial
} } sections with Spurrs. I routinely cut serial sections with Spurrs
without
} } resorting to any kind of glue. Probably the most important thing is
how the
} } block is faced. I cut the trapezoid with a really long (.6 to .9 mm
base)
} } and the top of the trapezoid is almost the same length. What's really
} } critical here is that those two cuts are parallel.
} } The two sides of the trapezoid are very short (.1mm). I cut the
sections
} } with a Diatome knife and on a Reichert Ultracut E with a cutting speed
of
} } 0.8mm per second and a thickness setting of 80nm. I am doing this in
a
} } small room with a blasting air vent in the ceiling. I have cut up a
} } cardboard box and stuck it into the ceiling tiles in such a way that
the air
} } is diverted away from the microtome. I can pick up 15 to 25 sections in
a
} } row on a 0.4 X 2mm copper slotted grid coated with just formvar or
} } formvar/carbon. I have never been any good at making the support films
my
} } self, so I buy the slotted grids precoated from Ted Pella (catalog #s
01706,
} } 01806, or 01816). Before picking up the ribbon, I dip a stick into
} } chloroform and wave that over the sections, and they expand (or relax,
} } depending on your point of view). I have a bunch of self locking
tweezers
} } and after picking up the sections, I leave the grid with the sections
on it
} } locked in the tweezers until they dry (if you put a wet slotted grid
down on
} } filter paper it might break the film). Good luck, Tim
} }
} } Timothy G. Schneider
} } Director of Electron Microscopy
} } Department of Pathology
} } Room 229 Jefferson Hall
} } Thomas Jefferson University
} } 1020 Locust St.
} } Philadelphia Pa. 19107
} } 215-503-4798 work
} } 610-613-8170 cellular
} } timothy.schneider-at-mail.tju.edu
}
}





_______________________________________________________
Tired of slow Internet? Get -at-Home Broadband Internet
http://www.home.com/xinbox/signup.html




From daemon Thu Dec 7 07:31:58 2000



From: Roger Moretz :      rcmoretz-at-excite.com
Date: Thu, 7 Dec 2000 05:27:45 -0800 (PST)
Subject: Objectives for AO microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greetings:

I have an old American Optical Spencer microscope (rescued from a trash
bin). It is a binoc, three objective turret (with 4x, 10x, 20x) and
built-in light source scope. I have loaned the scope to a young man who is
being home schooled, and he has become really interested in microscopy, and
aquatic biology. He is at the point that he needs at least a 40x objective,
and all I have are the 3 that came with the scope. The only numbers I can
find on the scope are 1063-1AA and 1235, and neither is specifically
designated as the model or serial numbers. Can anyone direct me to a source
for objectives for this type of microscope? These old scopes used a
standard thread and tube length, so maybe there are non-manufacturer
objectives that I can put on the scope. I haven't been able to look thru an
Edmund Scientific catalog (there's one around here somewhere), but I would
hope that either a collector or someone at Leica could provide some
additional info.

Thanks in advance.

Roger Moretz, Ph.D.
Dept of Toxicology
Boehringer Ingelheim Pharmaceuticals





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From daemon Thu Dec 7 09:06:18 2000



From: Ingo Daberkow :      ingo.daberkow-at-tvips.com
Date: Tue, 5 Dec 2000 19:51:05 -0600
Subject: Fw: Wavefront Reconstruction

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi James,

In principle, wave front reconstruction can be done by:

1) Off-axis holography: This methods need a special biprism for
overlapping of two beams (one passed the specimen, the other one passed
a hole). For the wavefront construction are two commercial programs
available - one from Gatan and the other one from our company. The group
around Prof. Hannes Lichte in Dresden is very active in this field - you
should contact him, if you want to know details.

2) Focal variation: This method reconstructs the wave front from a focal
series with very small defocus steps. For the reconstruction is at least
one commercial program available - you should contact Andreas Thust in
Juelich, Germany, if you want to know more about it. Also the group from
Prof. Dirk van Dyck in Antwerp is very active in this field.
The automatic acquisition of focal series is integrated in our programs
and we are supporting also the JEOL-4000 remote control, but we are
supporting only our cameras, no Gatan cameras. Sorry.

I apologize if I might have forgotten some references - I remember also
a poster from Michael O'Keefe in Philadelphia from this year, which was
related to this matter.

Regards,
Ingo

++++++++++++++++++++++++++++++++++++++++++++++++
Dr. Ingo Daberkow
Tietz Video and Image Processing Systems GmbH
D-82131 Gauting, Germany
Tel: +49-89-8506567
FAX: +49-89-8509488
Internet: www.tvips.com
Email: ingo.daberkow-at-tvips.com



-------- Original Message --------


forwarded from the imaging news group

"james patrick birrell" {jbirrell-at-students.uiuc.edu} wrote in message
news: {Pine.GSO.4.10.10012041635310.22505-100000-at-ux12.cso.uiuc.edu} ...
} I was wondering if anybody would know where I could obtain software to
} facilitate the acquisition/analysis of images to be used to perform
} wavefront reconstruction using images from a TEM. Specifically, I'm
} looking for any software compatable with a JEOL 4000ex microscope with a
} Gatan CCD camera.
} Thanks,
} James Birrell



From daemon Thu Dec 7 12:37:25 2000



From: Lesley Weston :      lesley-at-interchange.ubc.ca
Date: Thu, 7 Dec 2000 10:32:30 -0800 (PST)
Subject: Re: SEM - Choice of buffer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I have always understood that we use cacodylate buffer because it
penetrates tissue faster and further than other buffers, taking the
fixative with it. Plus, of course, the advantages that it does not form a
precipitate with osmium, and that if you need to store tissues after
fixing, then it discourages the growth of microorganisms. It is toxic, but
surely not as much so as glutaraldehyde or OsO4, which we can't really
avoid in EM. So, I would suggest we all go on using it, and take all the
proper precautions for this and the other toxic agents that we need.

Lesley Weston.



On Wed, 6 Dec 2000, Malcolm Haswell wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Bart
}
} I have used PIPES as a substitute for cacodylate in most routine procedures
} simply because it has none of the precipitation problems of phosphate
} buffers. I understand that there may be more extraction with cacodylate in
} TEM so I would have thought that it may be a better buffer for SEM (less
} chance of shrinkage?). I would assume that HEPES was similar but have no
} experience with it.
}
} These are my own feelings and I have seen no scientific evidence to support
} this - has anyone else?
}
}
} Malcolm Haswell
} e.m. unit
} School of Sciences
} University of Sunderland
} UK
}
} Bart De Pauw wrote:
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} } Hello listers,
} }
} } We use cacodylate buffer for our SEM samples. Is HEPES or PIPES buffer
} } an alternative for the toxic cacodylate ? Our samples are mainly animal
} } tissue.
} }
} } Bart De Pauw
} } Ghent University
} } Faculty of Veterinary Medicine
} } Department Morphology
} } Belgium
}
}
}




From daemon Thu Dec 7 12:37:26 2000



From: Todd Kostman :      kostman-at-vaxa.cis.uwosh.edu
Date: Thu, 07 Dec 2000 12:29:29 -0600
Subject: LN2 Dewars

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear EMers,

I recently inherited a 160L liquid nitrogen dewar that I use to supply LN2
to the scopes in our facility. Both the previous director and I feel that
we do not get our monies worth, so to speak, from this dewar. I had our
local supplier look at it and they say it is fine. Once it gets to 1/2
tank,the remaining LN vaporizes and bleeds off within a day. The last time
I had it filled, I calculated that I used about 50L of the 160L before it
went dry. Once it got down to half, I dumped the remaining LN into a 34L
dewar (and it is stll there). Does everyone have pretty much the same
experience with these 160L dewars?

Thanks for your help, and Merry Christmas to all microscopists (who are the
salt of the earth!)


Sincerely,


Todd

Dr. Todd A. Kostman
Assistant Professor of Biology and Microbiology
Director, Electron Microscopy Facility
University of Wisconsin Oshkosh
800 Algoma Boulevard
Oshkosh, WI 54901-8640
ph: (920) 424-3069
fax: (920) 424-1101
kostman-at-uwosh.edu





From daemon Thu Dec 7 12:53:18 2000



From: S. Kuehner :      kuehner-at-u.washington.edu
Date: Thu, 7 Dec 2000 10:50:53 -0800 (PST)
Subject: Re: LN2 Dewars

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Yes, I have also found 160L dewars NOT to be cost effective with only a
small user base. I switched to a 35L dewar many years ago. Even with the
more frequent deliveries I'm saving money over the 160L.

************************************************
### Free the Psoas ###
**************

Dr. Scott Kuehner kuehner-at-u.washington.edu
Dept. of Geological Sciences ph.206-543-8393
Box 351310 Fax 206-616-6873
The University of Washington
Seattle, Washington 98195-1310
************************************************

On Thu, 7 Dec 2000, Todd Kostman wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear EMers,
}
} I recently inherited a 160L liquid nitrogen dewar that I use to supply LN2
} to the scopes in our facility. Both the previous director and I feel that
} we do not get our monies worth, so to speak, from this dewar. I had our
} local supplier look at it and they say it is fine. Once it gets to 1/2
} tank,the remaining LN vaporizes and bleeds off within a day. The last time
} I had it filled, I calculated that I used about 50L of the 160L before it
} went dry. Once it got down to half, I dumped the remaining LN into a 34L
} dewar (and it is stll there). Does everyone have pretty much the same
} experience with these 160L dewars?
}
} Thanks for your help, and Merry Christmas to all microscopists (who are the
} salt of the earth!)
}
}
} Sincerely,
}
}
} Todd
}
} Dr. Todd A. Kostman
} Assistant Professor of Biology and Microbiology
} Director, Electron Microscopy Facility
} University of Wisconsin Oshkosh
} 800 Algoma Boulevard
} Oshkosh, WI 54901-8640
} ph: (920) 424-3069
} fax: (920) 424-1101
} kostman-at-uwosh.edu
}
}
}
}




From daemon Thu Dec 7 13:42:00 2000



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Thu, 7 Dec 2000 13:34:24 -0600
Subject: LN2 Dewars

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

We have had a similar experience here, going to a 160L dewar after having to
make a trip across campus with a 30L dewar everytime we needed LN2. The
huge discount on the 160L container means we're paying about the same per
liter actually used as we were before, plus we no longer have the
aggravation of having to go and get it.

One discovery we made, however, is that by transferring LN2 from the 160L
dewar to smaller, non-pressurized ones we are able to keep the nitrogen
much, much longer. We have 10L, 50L and 30L containers in our lab, so now
when a new 160L dewar is delivered, we fill up the smaller ones immediately,
then use the 160L until it's gone. The smaller dewars will keep LN2
literally for weeks with minimal losses. Initially we were averaging 60
usable liters per 160 delivered, but now we are probably up way past 100 (I
haven't actually figured it out.).

Good luck.

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/




-----Original Message-----
} From: Todd Kostman [mailto:kostman-at-vaxa.cis.uwosh.edu]
Sent: Thursday, December 07, 2000 12:29 PM
To: Microscopy Listserver


Dear EMers,

I recently inherited a 160L liquid nitrogen dewar that I use to supply LN2
to the scopes in our facility. Both the previous director and I feel that
we do not get our monies worth, so to speak, from this dewar. I had our
local supplier look at it and they say it is fine. Once it gets to 1/2
tank,the remaining LN vaporizes and bleeds off within a day. The last time
I had it filled, I calculated that I used about 50L of the 160L before it
went dry. Once it got down to half, I dumped the remaining LN into a 34L
dewar (and it is stll there). Does everyone have pretty much the same
experience with these 160L dewars?

Thanks for your help, and Merry Christmas to all microscopists (who are the
salt of the earth!)


Sincerely,


Todd

Dr. Todd A. Kostman
Assistant Professor of Biology and Microbiology
Director, Electron Microscopy Facility
University of Wisconsin Oshkosh
800 Algoma Boulevard
Oshkosh, WI 54901-8640
ph: (920) 424-3069
fax: (920) 424-1101
kostman-at-uwosh.edu





From daemon Thu Dec 7 13:52:43 2000



From: Tom Phillips :      PhillipsT-at-missouri.edu
Date: Thu, 7 Dec 2000 13:33:39 -0600
Subject: Re: SEM - Choice of buffer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I don't agree. I seriously doubt that the diffusion rate of
cacodylate and the "Good buffers" (i.e., the buffers like PIPES,
HEPES, MES, that were designed by Dr. Good) were ever compared since
they are from different eras. I am also not sure I understand how
the diffusion rate of a buffer component "takes the fixative with it"
at the molecular level. Arsenic buffers do inhibit bacterial growth
but I have had bacteria grow in cacodylate buffers. Furthermore, if
you have aldehydes or osmium in your buffer, you have all the
anti-bacterial agent you need. Cacodylate buffers can give off toxic
gases when acidified but more importantly, you are adding needlessly
to the toxic waste stream. Aldehydes will eventually react or
breakdown into non-toxic substances but the arsenic will remain a
problem forever. Cacodylate was originally selected based on its pKa
and non-reactivity with fixatives. Phosphate caused problems with
calcium precipitation and Tris buffers had amino groups that react
with the fixatives. The choices for buffers were very limited in the
old days. Dr. Good made a significant contribution in his
description of this family of buffers. Anatomists tend to be the
most old fashioned scientists in the world.



}
}
} I have always understood that we use cacodylate buffer because it
} penetrates tissue faster and further than other buffers, taking the
} fixative with it. Plus, of course, the advantages that it does not form a
} precipitate with osmium, and that if you need to store tissues after
} fixing, then it discourages the growth of microorganisms. It is toxic, but
} surely not as much so as glutaraldehyde or OsO4, which we can't really
} avoid in EM. So, I would suggest we all go on using it, and take all the
} proper precautions for this and the other toxic agents that we need.
}
} Lesley Weston.
}
}
}
} On Wed, 6 Dec 2000, Malcolm Haswell wrote:
}
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Bart
} }
} } I have used PIPES as a substitute for cacodylate in most routine procedures
} } simply because it has none of the precipitation problems of phosphate
} } buffers. I understand that there may be more extraction with cacodylate in
} } TEM so I would have thought that it may be a better buffer for SEM (less
} } chance of shrinkage?). I would assume that HEPES was similar but have no
} } experience with it.
} }
} } These are my own feelings and I have seen no scientific evidence to support
} } this - has anyone else?
} }
} }
} } Malcolm Haswell
} } e.m. unit
} } School of Sciences
} } University of Sunderland
} } UK
} }
} } Bart De Pauw wrote:
} }
} } } ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } } -----------------------------------------------------------------------.
} } }
} } } Hello listers,
} } }
} } } We use cacodylate buffer for our SEM samples. Is HEPES or PIPES buffer
} } } an alternative for the toxic cacodylate ? Our samples are mainly animal
} } } tissue.
} } }
} } } Bart De Pauw
} } } Ghent University
} } } Faculty of Veterinary Medicine
} } } Department Morphology
} } } Belgium
} }
} }
} }

--
Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)



From daemon Thu Dec 7 15:05:43 2000



From: Linda Fox :      LFOX1-at-wpo.it.luc.edu
Date: Thu, 07 Dec 2000 15:05:16 -0600
Subject: peripheral nerve voodoo

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Friends,
Once a gain I need that voodoo that you do so well.

A colleague is asking how to embed, freeze and frozen-section (cross section) a rat sciatic perpheral nerve. Several attempts have failed to give adequate structural detail. Any tips and tricks are welcome.

Linda Fox
lfox1-at-lumc.edu



From daemon Thu Dec 7 15:05:55 2000



From: Marie E. Cantino :      cantino-at-uconnvm.uconn.edu
Date: Thu, 7 Dec 2000 16:02:56 -0500
Subject: Re: LN2 Dewars

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We got similar results when we monitored nitrogen use 10 years ago, and
again when we retested earlier this year. We tried two different 160 liter
tanks, one of which was brand new, and never got more than about 80 liters
out. I eventually gave up and went to four 50 liter tanks, which we
receive at atmospheric pressure and tap with a withdrawal device that self
pressurizes to about 9 psi. The device has a rubber (now teflon for better
thermal properties) transfer hose, so there is little loss due to warming
the hose. We usually get 40-45 liters out of these.

The losses, as I understand them, are from

1. passive losses from the tank itself (static evaporation rate) which are
higher for the 160 liter tanks
2. losses due to cooling of the transfer tube (lower in the 50 liter tank
with no phase separator)
3. depressurization loss, which is higher in the 160 liter tank pressurized
to 20 psi than for the 50 L tank at 9 psi.

Obviously if you need high pressure or use high volumes, the 160 liter tank
is better. Also, with the 50 liter tanks you have to plan ahead because it
takes some time to pressurize the tanks, but otherwise the 50 liter option
works for us.

Marie

the} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Dr. Marie E. Cantino
Dept. of Physiology and Neurobiology, U-2131
University of Connecticut
Storrs, CT 06269-2131
Phone: 860-486-3588
Fax: 860-486-6369





From daemon Thu Dec 7 16:00:32 2000



From: michael shaffer :      epmalab-at-darkwing.uoregon.edu
Date: Thu, 7 Dec 2000 13:57:42 -0800
Subject: SEM: mystery CL

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


... just for fun ... anyone know what this is?

(hint: image is electron induced cathodo-luminescence ... the
material is natural quartz ... the colors are false but
representative)

happy holidays, shAf :o)

{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {}
Michael Shaffer, R.A. - mshaf-at-darkwing.uoregon.edu
Geological Science's Electron Probe Facility - University of Oregon
http://epmalab.uoregon.edu/





From daemon Thu Dec 7 16:02:33 2000



From: michael shaffer :      epmalab-at-darkwing.uoregon.edu
Date: Thu, 7 Dec 2000 14:00:21 -0800
Subject: SEM: mystery CL (oops)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


oops! ... forgot the URL ...*smile*...
http://epmalab.uoregon.edu/images/sem/mystery-CL.jpg


... just for fun ... anyone know what this is?

(hint: image is electron induced cathodo-luminescence ... the
material is natural quartz ... the colors are false but
representative)

happy holidays, shAf :o)

{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {}
Michael Shaffer, R.A. - mshaf-at-darkwing.uoregon.edu
Geological Science's Electron Probe Facility - University of Oregon
http://epmalab.uoregon.edu/





From daemon Fri Dec 8 03:37:50 2000



From: Malcolm Haswell :      malcolm.haswell-at-sunderland.ac.uk
Date: Fri, 08 Dec 2000 09:35:03 +0000
Subject: Re: LN2 Dewars

Contents Retrieved from Microscopy Listserver Archives
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Todd

we seem to be the exception. I expect to recover about 100L from a 160L dewar
over a two to three week period. I'm sure we once got a near record 110L out
but occasionally fall a little below the 100. We still therefore find the 160L
storage dewar more convenient because the alternative would require more space,
higher delivery cost and the need for multiple trolleys for several 25 litre
dewars. We can normally get nitrogen almost to the bottom of the 160L dewar,
providing that we don't leave less than about 10L in it (I'm guessing).
The only down-side is that it is a pressure vessel and requires regular
maintenance and testing to meet UK regulations, but this means that the spring
loaded pressure relief valve is regularly checked (as well as the burst disk
valve of course). It does however mean that if our 160L dewar ever fails its
tests that we will probably just buy more 25L dewars instead of replacing it.

Good luck

Malcolm Haswell
e.m. unit
School of Sciences
University of Sunderland
UK

Todd Kostman wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Dear EMers,
}
} I recently inherited a 160L liquid nitrogen dewar that I use to supply LN2
} to the scopes in our facility. Both the previous director and I feel that
} we do not get our monies worth, so to speak, from this dewar. I had our
} local supplier look at it and they say it is fine. Once it gets to 1/2
} tank,the remaining LN vaporizes and bleeds off within a day. The last time
} I had it filled, I calculated that I used about 50L of the 160L before it
} went dry. Once it got down to half, I dumped the remaining LN into a 34L
} dewar (and it is stll there). Does everyone have pretty much the same
} experience with these 160L dewars?
}
} Thanks for your help, and Merry Christmas to all microscopists (who are the
} salt of the earth!)
}
} Sincerely,
}
} Todd
}
} Dr. Todd A. Kostman
} Assistant Professor of Biology and Microbiology
} Director, Electron Microscopy Facility
} University of Wisconsin Oshkosh
} 800 Algoma Boulevard
} Oshkosh, WI 54901-8640
} ph: (920) 424-3069
} fax: (920) 424-1101
} kostman-at-uwosh.edu




From daemon Fri Dec 8 04:55:48 2000



From: chris smith (IACR-RES) :      chris.smith-at-bbsrc.ac.uk
Date: Fri, 8 Dec 2000 10:41:37 -0000
Subject: HP LJ IIIp - EDAX

Contents Retrieved from Microscopy Listserver Archives
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Hi,

Many thanks to all who replied to this query. I should have thought of the
EDAX site, senile dementia is really kicking in now. Just as well I'm being
thrown on the scrap heap at Christmas.

ttfn, Chris



From daemon Fri Dec 8 07:11:50 2000



From: Ken Tiekotter :      tiekotte-at-up.edu
Date: Fri, 8 Dec 2000 07:02:04 -0600
Subject: Re: Objectives for AO microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Roger,

I do not know if I can help, but I have a an excellent 50x oil AO
objective with a correction collar used on an AO MicroStar series compound
microscope. This is the scope with a grey finish. The numerical aperture
of this objective is 0.80 and with the correction collar exceeds the light
gathering capacity and resolution of any dry 40x or 63x dry objective.

Do you have any items you can perhaps trade, i.e., Zeiss objective lenses?

Let me know what you think.

Ken
-------------
Ken Tiekotter, Adjunct Professor
The University of Portland
Department of Biology
5000 N. Willamette Blvd.
Portland, OR 97203

Contact tel. number: 503-413-5391

On Thu, 7 Dec 2000, Roger Moretz wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Greetings:
}
} I have an old American Optical Spencer microscope (rescued from a trash
} bin). It is a binoc, three objective turret (with 4x, 10x, 20x) and
} built-in light source scope. I have loaned the scope to a young man who is
} being home schooled, and he has become really interested in microscopy, and
} aquatic biology. He is at the point that he needs at least a 40x objective,
} and all I have are the 3 that came with the scope. The only numbers I can
} find on the scope are 1063-1AA and 1235, and neither is specifically
} designated as the model or serial numbers. Can anyone direct me to a source
} for objectives for this type of microscope? These old scopes used a
} standard thread and tube length, so maybe there are non-manufacturer
} objectives that I can put on the scope. I haven't been able to look thru an
} Edmund Scientific catalog (there's one around here somewhere), but I would
} hope that either a collector or someone at Leica could provide some
} additional info.
}
} Thanks in advance.
}
} Roger Moretz, Ph.D.
} Dept of Toxicology
} Boehringer Ingelheim Pharmaceuticals
}
}
}
}
}
} _______________________________________________________
} Tired of slow Internet? Get -at-Home Broadband Internet
} http://www.home.com/xinbox/signup.html
}
}
}





From daemon Fri Dec 8 07:46:41 2000



From: tellis2-at-hallmark.com
Date: Fri, 8 Dec 2000 07:41:45 -0600
Subject: Liq. nitrogen tanks

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I receive my liquid nitrogen in 160 L tanks from our supplier and they
generally last from 24 to 30 days with me filling up the EDX dewar twice a
week (about 3-4 liters). I have had some tanks that only lasted two weeks
and after I told our supplier about that they said the leak valve was
stuck open and they replaced or adjusted the leak valve as needed. You
should get your local supplier to inspect your tank and maybe replace or
adjust the leak valve. I would not adjust it myself unless you really know
what you are doing since an explosion is possible without proper pressure
release.
Terry Ellis
Hallmark Cards Inc.
e-mail: tellis2-at-hallmark.com




From daemon Fri Dec 8 07:57:03 2000



From: Paula Sicurello :      patpxs-at-gwumc.edu
Date: Fri, 08 Dec 2000 08:57:59 -0500
Subject: I'm coming unglued

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Happy holidays oh great wise ones of microscopy!

(Apologies to John Lennon)


Imagine all day sectioning,
it isn't hard to do.
Putting sections on bare 200 mesh grids
and heat fixing under a light to boot.

Imagine all the sections coming off
while you do the stain.
Oh oh
You have to figure out what's wrong,
while racking your brain.
If you all can help,
you'll help me from going insane.


Any suggestions will be greatly appreciated. Thanks is advance!

Paula :-)

Paula Sicurello
George Washington Univ. Medical Center
Dept. of Pathology, Ross Hall rm 505
Electron Microscope Lab
2300 Eye St.
Washington, DC 20037
202-994-2930 phone
202-994-2518 fax




From daemon Fri Dec 8 08:10:58 2000



From: Sandra Hancock :      skperkin-at-vt.edu
Date: Fri, 08 Dec 2000 10:26:13 -0500
Subject: endothelial cell junctions

Contents Retrieved from Microscopy Listserver Archives
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Hi-

We have a post-doc that is interested in looking at tight junctions in
cultured endothelial cells. He has found a vague reference for using
silver stain to visualize endothelial cell junctions. Does anyone have any
experience with this technique? Can you provide some technical details or
point us to a reference? Thank you!!

Sandy Hancock
VMRCVM





From daemon Fri Dec 8 09:31:48 2000



From: Max Sidorov :      msv-at-nl.feico.com
Date: Fri, 8 Dec 2000 16:25:13 +0100
Subject: TEM - Updated: Contrast Transfer Function Freeware (ctfExplorer)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear All:

ctfExplorer has been updated.
New features/improvements:
1. Now it is possible to export graphs to text (tab-delimited).
2. List-tree with the microscopes is wider now.
3. Tab stops in dialogs arranged in a logical sequence.
4. Now it calculates the Lichte-defocus. (Useful for holography).
5. Additional slider-control is added: allows to change "magnification",
i.e. convergence. Just to show people why it is not always a good idea to
work at 1,000,000x and why some people do high resolution work at as low as
50,000x magnification.

Here is the link: http://clik.to/ctfexplorer (always use this link. it will
direct you to the right location).

Enjoy,

Max Sidorov
---
(until December 31 2000)
TEM Applications Specialist
FEI/Philips Electron Optics
Eindhoven, The Netherlands
e-mail: maxsidorov-at-bigfoot.com (mailto:maxsidorov-at-bigfoot.com)

----------Additional Info----------
Dear All:
I wrote a piece of software which I believe would be of interest to the
microscopy community.
It's a highly interactive program which calculates/displays the contrast
transfer function of TEMs. I know that there are similar programs floating
around but ctfExplorer does not only 1d but also 2d calculations/display
with 2-fold and 3-fold astigmatism imposed. There are other unique features
to it. All parameters (defocus, voltage, Cs, etc) can be changed
interactively.

DISCLAIMER: I do work for FEI/Philips Electron Optics. However, this
software is not an official product of FEI and FEI is not responsible for
its distribution/support. This software is freeware.

To the best of my knowledge, the software does not have any nasty bugs. It
is tested under Windows 95/98 and Windows NT4.

Please give it a try. Please direct your suggestions and comments to
maxsidorov-at-bigfoot.com
I hope you'll find the software useful.

Here is the link: http://clik.to/ctfexplorer

Enjoy,

Max Sidorov
---
TEM Applications Specialist
FEI/Philips Electron Optics
Eindhoven, The Netherlands
e-mail: maxsidorov-at-bigfoot.com






From daemon Fri Dec 8 09:46:33 2000



From: Matt Ervin :      mervin-at-arl.army.mil
Date: Fri, 8 Dec 2000 10:42:11 -0500
Subject: LN2 Dewars

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Todd-
I have not calculated how much LN2 I am able to deliver from my 160 L
dewar, but it must be about 100 L since it will last me a month or more.
Perhaps my dewar is of a better grade as it is also certified for liquid He
use. I suspect that much of my LN2 loss is in cooling down the 10 L dewar
I use to transport it to the EDX dewar. Are you using a high pressure, or
low pressure dewar (mine has ~24psi max)? The high pressure dewars I have
had have an internal coil for warming up some liquid to maintain the head
pressure. If your dewar has this, there should be valves for closing off
this pressure generation coil which will extend the storage time. By the
way, what does your fill gauge read when the dewar is empty? These gauges
are frequently very inaccurate. The fact that you could fit the remainder
in a 34 L dewar when your gauge said 1/2 full, indicates to me that your
gauge probably isn't accurate. I hope that something in there helps...
Matt




Todd Kostman {kostman-at-vaxa.cis.uwosh.edu} on 12/07/2000 01:29:29 PM





To: Microscopy Listserver {Microscopy-at-sparc5.microscopy.com}
cc:


Dear EMers,

I recently inherited a 160L liquid nitrogen dewar that I use to supply LN2
to the scopes in our facility. Both the previous director and I feel that
we do not get our monies worth, so to speak, from this dewar. I had our
local supplier look at it and they say it is fine. Once it gets to 1/2
tank,the remaining LN vaporizes and bleeds off within a day. The last time
I had it filled, I calculated that I used about 50L of the 160L before it
went dry. Once it got down to half, I dumped the remaining LN into a 34L
dewar (and it is stll there). Does everyone have pretty much the same
experience with these 160L dewars?

Thanks for your help, and Merry Christmas to all microscopists (who are the
salt of the earth!)


Sincerely,


Todd

Dr. Todd A. Kostman
Assistant Professor of Biology and Microbiology
Director, Electron Microscopy Facility
University of Wisconsin Oshkosh
800 Algoma Boulevard
Oshkosh, WI 54901-8640
ph: (920) 424-3069
fax: (920) 424-1101
kostman-at-uwosh.edu









From daemon Fri Dec 8 13:03:34 2000



From: Mary Gail Engle :      mgengle-at-pop.uky.edu
Date: Fri, 08 Dec 2000 13:57:38 -0500
Subject: coming unglued (sections, that is)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Paula,
I loved your poem! I too , had the same problem with sections falling off
grids while staining, especially if using the thin bar grids. There's just
not enough surface area for the sections to stick to. I solved the
problem by putting the grids in the 60 degree oven overnight before
staining. I haven't lost any sections since, and I cut both very large and
small sections. The time in the oven doesn't seem to adversely affect the
tissue.

Good luck,
Mary Gail Engle
Electron Microscopy & Imaging Facility
University of Kentucky



From daemon Fri Dec 8 13:03:55 2000



From: Paula Sicurello :      patpxs-at-gwumc.edu
Date: Fri, 08 Dec 2000 14:05:12 -0500
Subject: Thanks to all & a little clarification

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All,


Thanks to all who have already answered my plea. I think I need to make a few things clear. Me, in my effort to be witty forgot a few major details.


1. I'm cutting Spurr's embedded samples.

2. I'm cleaning the samples in a dilute acetic acid followed by an acetone chaser.

3. I've been heat fixing the grids/sections under a light for about 10 minutes.

4. I've been doing this for that past 10 months and things worked great until 3 weeks ago. I'm new to this area
(Washington, DC) having been in California doing EM for years. Could this be a climate/weather related
problem?

5. I will use formvar if I have to. Has anyone just dipped grids in formvar, instead of casting a film, to make the grids
a little sticky? I inherited a bunch of filthy formvar with water & junk in it and it takes about a month to get supplies.

6. I haven't changed my supplier of grids or Spurr's components. So I think that things should be OK on the quality
control end.


A lot of the suggestions I received are very good, but I have already tried most of them. Any bizarre rituals that I must do? I WILL NOT swing a dead cat over my head, but I might try other things. EM is basically voodoo, after all.

Thanks again,

Paula :-)

Paula Sicurello
George Washington Univ. Medical Center
Dept. of Pathology, Ross Hall rm 505
Electron Microscope Lab
2300 Eye St.
Washington, DC 20037
202-994-2930 phone
202-994-2518 fax




From daemon Fri Dec 8 13:19:10 2000



From: DarrenScott Gray :      microscopy-at-Thunderdome.zzn.com
Date: Fri, 8 Dec 2000 14:13:59 -0500
Subject: please add me to the mailing list

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


{html} {head} {meta Name='keywords' Content='commtouch, pronto, mail, free email, free, branded, web based, free web based email, communications, internet, software, advertising banners, e-mail, free software'} {/head} {body } {div align='left'} {font } {blockquote} {blockquote} {TT} Hello, {BR}
{BR}
Please add me to the microscopy discussion group. {BR}
{BR}
Thanks, {BR}
Darren {BR}
{BR}
{/TT} {br} {br} {font} {p align=left} {br} Get your Free E-mail at http://thunderdome.zzn.com {br} ____________________________________________________________ {br} Get your own FREE Web and POP E-mail Service in 14 languages at http://www.zzn.com. {br} {/blockquote} {/blockquote} {/div} {/font} {/body} {/html}



From daemon Fri Dec 8 13:37:14 2000



From: Leona Cohen-Gould :      lcgould-at-mail.med.cornell.edu
Date: Fri, 8 Dec 2000 14:34:19 -0500
Subject: Re: I'm coming unglued

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Paula,
It is horrible to see naked grids come off the stain. We've probably
all been there. Here are my auggestions:

1. Make sure that your grids are clean to start. You can dip them in
acetone or ethanol (absolute) and then water and dry thoroughly.

2. Make sure that the sections have fully dried onto the grids. If
you're not rushed, just let them dry overnight. If you are rushed
(probably the usual situation), you can lay the grids out on a clean
glass slide that is resting on a warm (NOT HOT) hot plate (around
35-40deg.C) for a few minutes.

3. And as wierd as this seems....make sure that the water you are
using to make up your stains and to wash the grids is really clean
(deionized or double distilled or microfiltered). I had a friend to
used to come over to my building to fill a small carboy with water
because my dept. had better water treatment than hers. She swore
that her sections fell off when she used their water.

4. Maybe the simplest life saver....buy a "Coat-Quick G" grid coating
pen. It deposits a small amount of the "secret formula" adhesive
onto the grid bars, without blocking the spaces or causing problems
with dirt/stain deposition. I frequently cut enormous sections (by
EM standards) and this has been a blessing. I know that Electron
Microscopy Sciences sells them. Other companies probably do too.
Look in you favorite vendor's catalogue.


(Disclaimer: I have no financial interests in EMS)

Good luck,

Lee
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175



From daemon Fri Dec 8 13:43:11 2000



From: Gib Ahlstrand :      giba-at-puccini.cdl.umn.edu
Date: Fri, 8 Dec 2000 13:49:39 -0600 (CST)
Subject: Re: I'm coming unglued

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Responding to the message of {sa30a2a7.040-at-gwise.gwumc.edu}
from "Paula Sicurello" {patpxs-at-gwumc.edu} :

Paula,


Imagine........ a magic solution........

GRID CLEANING SOLUTION

Clean your uncoated grids each time you set up to do sectioning, because newly
cleaned copper grids will oxidize within a day and sections will no longer stick
to them very well during staining and rinsing.


*ADD 40 ml CONCENTRATED HCL TO 400 ml DISTILLED WATER

*ADD 40 ml ACETONE TO THE ABOVE

*DILUTE TO 500 ml FINAL VOLUME

*SONICATE GRIDS IN THIS SOLUTION IN A 50 ml BEAKER FOR 1-2 MINUTES

*DISCARD USED SOLUTON DOWN DRAIN AND RINSE/SONICATE GRIDS FOR 30 SECONDS TWICE
WITH 100% ACETONE

*DUMP GRIDS ONTO CLEAN FILTER PAPER TO DRY

* ITS TRADITIONAL TO COLLECT SECTIONS ON THE DULL SIDE OF THE GRIDS.


It works well for us here, and is used routinely every day. Good luck,

Gib

}
} Happy holidays oh great wise ones of microscopy!
}
} (Apologies to John Lennon)
}
}
} Imagine all day sectioning,
} it isn't hard to do.
} Putting sections on bare 200 mesh grids
} and heat fixing under a light to boot.
}
} Imagine all the sections coming off
} while you do the stain.
} Oh oh
} You have to figure out what's wrong,
} while racking your brain.
} If you all can help,
} you'll help me from going insane.
}
}
} Any suggestions will be greatly appreciated. Thanks is advance!
}
} Paula :-)
}
} Paula Sicurello
} George Washington Univ. Medical Center
} Dept. of Pathology, Ross Hall rm 505
} Electron Microscope Lab
} 2300 Eye St.
} Washington, DC 20037
} 202-994-2930 phone
} 202-994-2518 fax


Gib Ahlstrand
Electron Optical Facility, University of Minnesota, Dept. Plant Pathology
495 Borlaug Hall, St. Paul, MN. USA. 55108 (612)625-8249
612-625-9728 FAX, giba-at-puccini.cdl.umn.edu
http://biosci.umn.edu/MIC/consortium.html




From daemon Fri Dec 8 15:45:10 2000



From: Erickson, Lauren -Belle Plaine :      lrerickson-at-imcglobal.com
Date: Fri, 8 Dec 2000 15:26:41 -0600
Subject: SEM of Potash

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I'm looking for methods of preparing compacted potassium chloride fertilizer
for the SEM. We believe that grain size is indicative of compaction quality
in our process, however we're not sure of the best way to prepare the
samples. The compactor flake is approximately 2"x1"x0.25". Any advice
would be much appreciated.

Thanks,
Lauren Erickson
lrerickson-at-imcglobal.com



From daemon Fri Dec 8 17:18:22 2000



From: Gordon Couger :      gcouger-at-couger.com
Date: Fri, 8 Dec 2000 17:12:26 -0600
Subject: Wanted Wollaston prisms for Reichert Zetopan DIC scope

Contents Retrieved from Microscopy Listserver Archives
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I have a freind that need then main prism slide containing the Wollaston
prism for Reichert Zetopan DIC scope. This is the slide going in the upper
body of the scope. He also needs the polorizor that fits over the light
soruce but that is of much less importance beause it can be easily made.
While the prism is constructed of unobtainium.

He is willing to pay a reasonable price for the part.

If any of you have a spare or left over you could make a very dissapointed
man a lot happier.

Gordon
Gordon Couger gcouger-at-couger.com
Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00





From daemon Fri Dec 8 17:18:27 2000



From: sghoshro-at-nmsu.edu
Date: Fri, 8 Dec 2000 16:11:21 -0700 (MST)
Subject: Re: I'm coming unglued

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Paula,

I have noticed that in some cases the kind of grid you use is quite
important. I had similar problems with Gilder grids when using Spurr
embedded plant tissue sections. Later I switched to Veco grids, the
problem went away.

Good luck,

Soumitra


*************************************************************
Soumitra Ghoshroy Ph.D.
Electron Microscopy Lab and Fluorescence Imaging Facility
Department of Biology
Box 3EML
New Mexico State University
Las Cruces, NM 88003
Tel: 505-646-3600
Fax: 505-646-5665
e-mail:sghoshro-at-nmsu.edu
http://confocal.nmsu.edu/eml





From daemon Fri Dec 8 18:12:07 2000



From: JNunnari-at-aol.com
Date: Fri, 8 Dec 2000 19:08:51 EST
Subject: re:endothelial junctions

Contents Retrieved from Microscopy Listserver Archives
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Sandy Hancock
Try the following reference;
Zand, Underwood, Nunnari, Majno and Joris,
Endothelium and 'silver lines'. An electron microscopic study. 1982 Virchows
Archiv of Path Anat. 395: 133-144
Good luck
John



From daemon Fri Dec 8 19:03:23 2000



From: Roger Moretz :      rcmoretz-at-excite.com
Date: Fri, 8 Dec 2000 18:58:20 -0600
Subject: Re: Objectives for AO microscope

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Thanks to everyone for the response. It will take me a little while to
fully sort through everything, but I appreciate the help.

Roger Moretz, Ph.D.
Dept of Toxicology
Boehringer Ingelheim Pharmaceuticals

On Fri, 8 Dec 2000 02:31:47 -0800 (PST), Ken Tiekotter wrote:

} Dear Roger,
}
} I do not know if I can help, but I have a an excellent 50x oil AO
} objective with a correction collar used on an AO MicroStar series
compound
} microscope. This is the scope with a grey finish. The numerical aperture
} of this objective is 0.80 and with the correction collar exceeds the
light
} gathering capacity and resolution of any dry 40x or 63x dry objective.
}
} Do you have any items you can perhaps trade, i.e., Zeiss objective
lenses?
}
} Let me know what you think.
}
} Ken
} -------------
} Ken Tiekotter, Adjunct Professor
} The University of Portland
} Department of Biology
} 5000 N. Willamette Blvd.
} Portland, OR 97203
}
} Contact tel. number: 503-413-5391
}
} On Thu, 7 Dec 2000, Roger Moretz wrote:
}
} }
------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America

} } To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
} } On-Line Help
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} }
-----------------------------------------------------------------------.
} }
} }
} } Greetings:
} }
} } I have an old American Optical Spencer microscope (rescued from a trash
} } bin). It is a binoc, three objective turret (with 4x, 10x, 20x) and
} } built-in light source scope. I have loaned the scope to a young man
who is
} } being home schooled, and he has become really interested in microscopy,
and
} } aquatic biology. He is at the point that he needs at least a 40x
objective,
} } and all I have are the 3 that came with the scope. The only numbers I
can
} } find on the scope are 1063-1AA and 1235, and neither is specifically
} } designated as the model or serial numbers. Can anyone direct me to a
source
} } for objectives for this type of microscope? These old scopes used a
} } standard thread and tube length, so maybe there are non-manufacturer
} } objectives that I can put on the scope. I haven't been able to look
thru an
} } Edmund Scientific catalog (there's one around here somewhere), but I
would
} } hope that either a collector or someone at Leica could provide some
} } additional info.
} }
} } Thanks in advance.
} }
} } Roger Moretz, Ph.D.
} } Dept of Toxicology
} } Boehringer Ingelheim Pharmaceuticals
} }
} }
} }
} }
} }
} } _______________________________________________________
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From daemon Fri Dec 8 19:30:42 2000



From: Ronald Austin :      rla-at-mindspring.com
Date: Fri, 8 Dec 2000 19:27:56 -0600
Subject: Re: I'm coming unglued

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Paula
Try this cleaning procedure for your copper grids:
1) Place your grids in a clean beaker, 100ml size will do.
2) Cover the gird with Glacial Acetic Acid, approximately 20cc (the idea
here is to get the grim and grease
off the grids not to eat the metal away)
3) Sonicate the grids in this acid for about 5 to 10 minutes.
4) Rinse in 100% acetone until the smell of the acetic acid is gone.
5) Do a final rinse in 100% ETOH (denatured is ok) and pipette the ETOH off
and invert the beaker into a glass petri dish that has a clean whatman
filter paper in it and place in your 60 degree over for an hour or more. The
grids will fall on to the filter paper. Cover the bottom of the glass with a
top.
The grids will keep without father cleaning until they are all used up. I
use a glass petri dish because there is less static charge with glass.

Ron Austin
Dept. of Pathology
LSU Medical Center
Shreveport, LA
rla-at-mindspring.com
318-675-4557




From daemon Fri Dec 8 19:41:16 2000



From: Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 08 Dec 2000 17:38:09 -0800
Subject: Re: SEM of Potash

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I'd just try a simple first shot approach of putting some of
the particles on a sticky tab, and coating it with about
60A of Au/Pd. Then, take a look at it under the SEM.
Based on this imaging, other approaches may or may
not be necessary.

It may or may not make a difference relative to the
type of SEM that you are using (FESEM vs. thermionic).
I would think that these are rather large specimens.
Either system should do a nice job.

gary g.


At 01:26 PM 12/8/00, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From daemon Sat Dec 9 09:10:03 2000



From: Robert H. Olley :      r.h.olley-at-reading.ac.uk
Date: Sat, 9 Dec 2000 14:59:05 +0000 (GMT Standard Time)
Subject: Flying grids and sticking stubs

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} From time to time, someone opens a grid storage box in our lab, and
because of the static that has built up on the plastic, the grids fly out
and are randomized. We have a Zerostat gun next to the TEM, but our
laboratory/office space is fragmented worse than a SWAP file, and one
can't simply reach for the ion gun every time one opens a grid box. Does
anyone (a) have any simple solutions to this problem (b) know of a
supplier of grid boxes made of a different plastic that doesn't charge up
so horribly?

In the longer term, would there be scope for making grid boxes out of a
plastic loaded with a filling to make it conductive enough for charges to
slowly leak away, so avoiding the problem altogether?

And on a similar theme, does anyone know of storage boxes for SEM stubs
into which the stubs can fit easily, i.e. not having to be rammed in?

+------------------------------------------------------------------------+
| Robert H.Olley Phone: |
| J.J.Thomson Physical Laboratory {direct line +44 (0) 118 9318572 |
| University of Reading {University internal extension 7867 |
| Whiteknights Fax +44 (0) 118 9750203 |
| Reading RG6 6AF Email: R.H.Olley-at-reading.ac.uk |
| England URL: http://www.reading.ac.uk/~spsolley |
+------------------------------------------------------------------------+







From daemon Sat Dec 9 12:23:01 2000



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Sat, 09 Dec 2000 22:01:46 -0500
Subject: TEM and SEM storage box problems

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Antistatic sprays are available from electronic components suppliers
such as Farnell and RS Components. These are mainly intended to
de-static the plastic covers of ammeters, etc. to stop them
influencing the readings.

You could also try coating the interior surfaces of your box with a
thin layer of carbon or gold next time you have the coater running.

Chris



Date sent: Sat, 9 Dec 2000 14:59:05 +0000 (GMT Standard Time)
} From: "Robert H. Olley" {r.h.olley-at-reading.ac.uk}
To: Microscopy Newsgroup {Microscopy-at-sparc5.microscopy.com}
Copies to: # {r.h.olley-at-reading.ac.uk}


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Robert Olley wrote:
================================================================
} From time to time, someone opens a grid storage box in our lab, and because
of the static that has built up on the plastic, the grids fly out and are
randomized. We have a Zerostat gun next to the TEM, but our
laboratory/office space is fragmented worse than a SWAP file, and one can't
simply reach for the ion gun every time one opens a grid box. Does anyone
(a) have any simple solutions to this problem (b) know of a supplier of grid
boxes made of a different plastic that doesn't charge up so horribly?

In the longer term, would there be scope for making grid boxes out of a
plastic loaded with a filling to make it conductive enough for charges to
slowly leak away, so avoiding the problem altogether?

And on a similar theme, does anyone know of storage boxes for SEM stubs into
which the stubs can fit easily, i.e. not having to be rammed in?
=================================================================
There are a number of grid boxes being molded today, some of which are being
molded with plastics that have had added antistatic additives. The SPI
Slide-A-Grid™ storage boxes (see website below) are molded with such
antistatic additives. This same box is offered (but under other names) by
some of the other leading firms offering these kinds of products to EM users
. However, under certain conditions, apparently humidity related, even
these boxes reportedly can develop a charge.

For SEM storage boxes, there are two approaches to the "base plate" that
sits in the bottom of each box, the first being a "hard" plastic such as
polypropylene and the other being with a "soft" plastic, specifically an
elastomeric plastic. SPI Supplies has offered storage boxes with the soft
plastic for some number of years, the advantage being that the mounts do fit
in easily and do not have to be "rammed" in. I do not know of anyone else
who is molding mounting plates out of the soft "elastomeric plastic" (but of
course I could be wrong about that).

Note: One of the biggest sources of misfit between the storage boxes and
the 3/8" (9.5 mm) round mounts is that boxes molded in the USA tend to be
made for 3/8" diameter mounts and boxes molded elsewhere seem to be made for
10 mm diameter mounts. The problem arises when one tries to put a 10 mm
round mount into a box designed for the 3/8"round mounts. If the "ramming"
problem you described could be this kind of a problem, a mixing up of
boxes/mounts might be the reason for the described difficulty. The flip-
side of this problem is when 3/8" mounts are put into boxes molded in Europe
to take 10 mm round mounts, and the result is, the mounts fall out when the
box is tipped.

Disclaimer: SPI Supplies offers boxes for the storage of both TEM grids and
SEM mounts, the details of which can be found on our website below.

Chuck
============================================

Charles A. Garber, Ph. D. Ph: 1-610-436-5400
President 1-800-2424-SPI
SPI SUPPLIES FAX: 1-610-436-5755
PO BOX 656 e-mail:cgarber-at-2spi.com
West Chester, PA 19381-0656 USA
Cust.Service: spi2spi-at-2spi.com

Look for us!
########################
WWW: http://www.2spi.com
########################
============================================









From daemon Sun Dec 10 03:20:44 2000



From: Trevor Sewell :      sewell-at-uctvms.uct.ac.za
Date: Sun, 10 Dec 2000 10:16:12 +0200
Subject: EM position vacant in Cape Town

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UNIVERSITY OF CAPE TOWN

TECHNICAL OFFICER/SENIOR TECHNICAL OFFICER/CHIEF TECHNICAL OFFICER
ELECTRON MICROSCOPE UNIT

If you have experience in electron microscopy and the use of
computers, then we invite you to apply for this post in a fast-moving
and ever-changing environment geared to servicing research needs.

The Electron Microscope Unit (EMU) primarily serves researchers in the
faculties of Science, Engineering and the Built Environment and Health
Sciences at UCT. The EMU aims to be the foremost provider of such
services in the Western Cape. Currently, the Unit has two scanning
and two transmission electron microscopes as well as a number of light
microscopes and will take delivery of a cryo-TEM early next year.

Duties of the appointee will include instrument operation, sample
preparation, user training and laboratory management.

The level of appointment will depend on the qualifications and the
experience of the successful candidate. Therefore, the remuneration
package, including benefits, is negotiable between R75 000 - R156 100
a year.

Please send a letter of application plus your CV (including the names,
postal/email addresses, telephone/fax numbers of 2 referees) to
Associate Professor Trevor Sewell, Director: Electron Microscope Unit,
UCT, Rondebosch, 7701 by 8 January 2001; tel: (021) 650-2817; fax:
689-1528; email: sewell-at-uctvms.uct.ac.za



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M97!A {F%T:6]N+"!U {V5R('1R86EN:6YG(&%N9"!R;W5T:6YE(&UA:6YT96YA
M;F-E+B!4:&4-at-16QE8W1R;VX-"DUI8W)O {V-O {&4-at-56YI="!S965S('1H92!A
M {'!O:6YT;65N="!O9B!A(&YE=R!P97)S;VX-at-87,-at-86X-"F]P {&]R='5N:71Y
M('1O(&9O8W5S(&]N(&9U='5R92!P;&%N;F5D(&%C=&EV:71I97,-at-*'=H:6-H
M(&EN8VQU9&4-"G-E=F5R86P-at-87-P96-T {R!O9B!C {GEO+6UI8W)O {V-O {'DI
M(&%N9"!W:6QL(&QO;VL-at-9F]R(&$-at- {&5R {V]N#0IW:71H(&EN=&5R97-T(&%N
M9"!E} '!E {FEE;F-E(&EN(&5L96-T {F]N(&UI8W)O {V-O {'D-at-=VAO('=I;&P-
M"F9U {G1H97(-at-=&AE(&UI {W-I;VX-at-;V8-at-=&AE(%5N:70-at-86YD('=H;W-E('1A
M;&5N=',-at-8V]M {&QE;65N= T*=&AO {V4-at-;V8-at-=&AE(&]T:&5R('-T869F+-at-T*
M#0I&=7)T:&5R(&EN9F]R;6%T:6]N(&%B;W5T('1H92!5;FET(&UA} 2!B92!O
M8G1A:6YE9"!F {F]M(&]U {B!W96(-"G-I=&4-at-870Z#0IH='1P.B\O=W=W+G5C
5="YA8RYZ82]D97!T {R]E;74-"-at-T*
`
end




From daemon Sun Dec 10 10:16:33 2000



From: Jim at ProSciTech :      jim-at-proscitech.com
Date: Sun, 10 Dec 2000 09:58:34 -0600
Subject: RE: Flying grids and sticking stubs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


During my laboratory days I never worked in a room were static was that bad -
and that included Canadian winters. Carpet and poor humidification can make a
static problem worse.
I never liked the boxes with a sliding lid for another reason: If they dropped
when open from a modest height several grids may escape and that is really the
same problem.
Probably all of the EM suppliers carry grids boxes that hold 24 grids and have
a rotating lid with two apertures. Only one grid at a time is can to escape.
Presto: No more feral grids.

I'm sure that "smarter" stub boxes could be designed, but would people buy
them
at 2 or 3 times the price?
We (and others) have a single SEM mount Storage/mailer and this is much better
for holding pin type mounts.
Wouldn't is be lovely if all EM manufacturers used the same (I vote for pin
type) mounts.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Sunday, December 10, 2000 12:59 AM, Robert H. Olley
[SMTP:r.h.olley-at-reading.ac.uk] wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
}
} } From time to time, someone opens a grid storage box in our lab, and
} because of the static that has built up on the plastic, the grids fly out
} and are randomized. We have a Zerostat gun next to the TEM, but our
} laboratory/office space is fragmented worse than a SWAP file, and one
} can't simply reach for the ion gun every time one opens a grid box. Does
} anyone (a) have any simple solutions to this problem (b) know of a
} supplier of grid boxes made of a different plastic that doesn't charge up
} so horribly?
}
} In the longer term, would there be scope for making grid boxes out of a
} plastic loaded with a filling to make it conductive enough for charges to
} slowly leak away, so avoiding the problem altogether?
}
} And on a similar theme, does anyone know of storage boxes for SEM stubs
} into which the stubs can fit easily, i.e. not having to be rammed in?
}
} +------------------------------------------------------------------------+
} | Robert H.Olley Phone: |
} | J.J.Thomson Physical Laboratory {direct line +44 (0) 118 9318572 |
} | University of Reading {University internal extension 7867 |
} | Whiteknights Fax +44 (0) 118 9750203 |
} | Reading RG6 6AF Email: R.H.Olley-at-reading.ac.uk |
} | England URL: http://www.reading.ac.uk/~spsolley |
} +------------------------------------------------------------------------+
}
}
}
}





From daemon Sun Dec 10 11:06:58 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Sun, 10 Dec 2000 10:58:03 -0600
Subject: M&M 2001 Meeting August 5-9 , 2001

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Colleagues....

On behalf of the Microscopy Society of America and the Microbeam Analysis
Society,
we invite you to attend Microscopy and Microanalysis 2001, August 5-9, in
Long Beach,
California. The program chair, Bob Price, and Co-Chairs Edgar Voelkl (MSA) and
Inga Holl Musselman (MAS), have assembled another excellent technical program
that continues the tradition of exciting and current presentations at the
meetings.
An outstanding list of invited speakers highlights the symposia in a
number of key
areas. Special symposia this year again cover a wide range, including
"Microscopy
and Microanalysis in the Real World," a special biological symposium honoring
Dr. Inou*, and "Atom Location by Channeling Enhancement of X-Ray and EELS
Signals"
among others, plus a wide variety of applications in biology and materials
research.

Both invited and contributed abstracts are critical to the success of these
symposia,
so we urge you to submit your work. Remember that the abstract deadline is
February 15th, 2001.

A special Pre-meeting Congress, "Imaging Life: From Cells to Whole
Animals," as well
as several workshops and short courses on important, basic techniques will
precede
the meeting. Special symposia, tutorials and presentations sponsored by the
Technologists'
Forum, the MSA Education Committee, and various commercial exhibitors will
be held
during the course of the meeting. There are also Presidential Happenings,
ceremonies
for Award Winners, and the world's largest display of microscopes and
related equipment.

Our Local Arrangements Committee, headed by Robert Koch, has arranged an
excellent
venue at the Long Beach Convention Center for both the scientific sessions
and the exhibits.
They have also arranged a memorable program of social events including a
Sunday night
reception on the Queen Mary, the annual golf tournament, a harbor cruise,
and a large
number of options for enjoying the city and the surrounding area (don't
forget the theme parks!).
With its rich history, wealth of educational institutions and active
industry, Long Beach
provides a wonderful context for this meeting, and we urge you to come to
learn, teach,
share and above all to enjoy. Whatever your connection to microscopy, we
look forward
to welcoming you to Long Beach in August at Microscopy and Microanalysis 2001.

Ron Anderson, President
Microscopy Society of America


Richard Linton, President
Microbeam Analysis Society

--------------------------

The program is as usual extensive and detailed information can be found
on the WWW page.

http://www.msa.microscopy.com/MSAMeetings/MM01/MMHomePage.html



Cheers....
Nestor
Your Friendly Neighborhood SysOp.

-------------------------






From daemon Sun Dec 10 18:38:59 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Sun, 10 Dec 2000 17:56:16 -0600
Subject: Administrivia: November Archives On-Line

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Colleagues

The Microscopy Listserver Archives from November are
now on-line.

http://www.msa.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Nestor
Your Friendly Neighborhood SysOp





From daemon Sun Dec 10 18:39:00 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Sun, 10 Dec 2000 18:25:32 -0600
Subject: Administrivia: November Archives On-Line

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Colleagues

The Microscopy Listserver Archives from November are
now on-line.

http://www.msa.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Nestor
Your Friendly Neighborhood SysOp
6:25 PM CST 12/10/00





From daemon Sun Dec 10 19:34:22 2000



From: Gordon Couger :      gcouger-at-couger.com
Date: Sun, 10 Dec 2000 19:24:41 -0600
Subject: Re: Flying grids and sticking stubs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I have fought static problems in wide variety of problems and three things
help. Grounding with metal, raising the humidity to 50% and spraying the
area or items with antistatic products designed for laundry. Downy was the
one I used. Temporary relief can be had by spraying the area with water
from Windex like spray bottle. This only last for a few minutes to an hour
at most.

I am sure fabric antistatic spray would not be acceptable in many places
in a lab. But floors and tables might benifit from them.

Spraying carpets with antistatic spray once a month work for us in 15 to
20% humidity.

Your mileage my vary.
Gordon
Gordon Couger gcouger-at-couger.com
Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00

} From: "Jim at ProSciTech" {jim-at-proscitech.com}
} During my laboratory days I never worked in a room were static was that
bad -
} and that included Canadian winters. Carpet and poor humidification can
make a
} static problem worse.
} I never liked the boxes with a sliding lid for another reason: If they
dropped
} when open from a modest height several grids may escape and that is
really the
} same problem.
} Probably all of the EM suppliers carry grids boxes that hold 24 grids
and have
} a rotating lid with two apertures. Only one grid at a time is can to
escape.
} Presto: No more feral grids.
}
} I'm sure that "smarter" stub boxes could be designed, but would people
buy
} them
} at 2 or 3 times the price?
} We (and others) have a single SEM mount Storage/mailer and this is much
better
} for holding pin type mounts.
} Wouldn't is be lovely if all EM manufacturers used the same (I vote for
pin
} type) mounts.
} Cheers
} }
} } } From time to time, someone opens a grid storage box in our lab, and
} } because of the static that has built up on the plastic, the grids fly
out
} } and are randomized. We have a Zerostat gun next to the TEM, but our
} } laboratory/office space is fragmented worse than a SWAP file, and one
} } can't simply reach for the ion gun every time one opens a grid box.
Does
} } anyone (a) have any simple solutions to this problem (b) know of a
} } supplier of grid boxes made of a different plastic that doesn't charge
up
} } so horribly?
} }
} } In the longer term, would there be scope for making grid boxes out of
a
} } plastic loaded with a filling to make it conductive enough for charges
to
} } slowly leak away, so avoiding the problem altogether?
} }
} } And on a similar theme, does anyone know of storage boxes for SEM
stubs
} } into which the stubs can fit easily, i.e. not having to be rammed in?
} }
}
}





From daemon Mon Dec 11 06:21:42 2000



From: John.Catino-at-mineralstech.com
Date: Mon, 11 Dec 2000 07:13:01 -0500
Subject: Kevex Quantum

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Kevex Quantum EDS system with Sesame drive is available for no charge for
pick-up in Easton, PA. The detector is NOT included. System has Syquest
drives and monitor is in need of repair.




**********************************************************************
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the system manager.

This footnote also confirms that this email message has been swept by
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From daemon Mon Dec 11 11:12:32 2000



From: mark brady :      brady-at-MMM.COM
Date: Mon, 11 Dec 2000 11:04:52 -0600
Subject: Counting and mounting media

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am doing some bacterial counting work. My basic method
involves taking some bacteria in a measured quantity of
suspension and running it thru an anodisc (aluminum oxide)
filter having .2 micron pores.

My problem is this: when mounting the filter on a slide, I find
that the disc is almost dry before I can mount it. I have tried
adding water and or oil below and above the filter. Water
sometimes works but it is a very tricky matter because the
water may redistribute the bacteria when applied. This affects
my sampling since the bacteria may no longer be uniform.

The same problem occurs with oil. Furthermore, the oil, if
applied to a filter with some water remaining, gives a blurry
image, perhaps due to the oil-water mix.

I am also concerned with air bubbles. Is there a method by
which one can prevent air bubbles under, over and in the
pores of the filter?

-Mark





From daemon Mon Dec 11 11:18:48 2000



From: Tony Garratt-Reed :      tonygr-at-mit.edu
Date: Mon, 11 Dec 2000 12:14:25 -0500
Subject: Re: Flying grids and sticking stubs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I would *STRONGLY* recommend against using any anti-static spray on grid
boxes themselves unless you are certain of the components of the spray. I
remember one batch of grid boxes we had to throw away because the
formulation of the plastic mix during manufactur was evidently wrong, and
we got horrendous contamination of samples which had been stored in the
boxes. We don't even touch the tweezers we are going to use for our
samples with our bare hands! I would never spray some unknown material
intended for some other, much less critical (in terms of cleanliness)
application, anywhere near anything related to the samples themselves.

Tony G-R


}
} Antistatic sprays are available from electronic components suppliers
} such as Farnell and RS Components. These are mainly intended to
} de-static the plastic covers of ammeters, etc. to stop them
} influencing the readings.
}
} You could also try coating the interior surfaces of your box with a
} thin layer of carbon or gold next time you have the coater running.
}
} Chris
}
}

} }
} } } From time to time, someone opens a grid storage box in our lab, and
} } because of the static that has built up on the plastic, the grids fly out
} } and are randomized. We have a Zerostat gun next to the TEM, but our
} } laboratory/office space is fragmented worse than a SWAP file, and one
} } can't simply reach for the ion gun every time one opens a grid box. Does
} } anyone (a) have any simple solutions to this problem (b) know of a
} } supplier of grid boxes made of a different plastic that doesn't charge up
} } so horribly?
} }
} } In the longer term, would there be scope for making grid boxes out of a
} } plastic loaded with a filling to make it conductive enough for charges to
} } slowly leak away, so avoiding the problem altogether?
} }
} } And on a similar theme, does anyone know of storage boxes for SEM stubs
} } into which the stubs can fit easily, i.e. not having to be rammed in?
} }


* * * * * * * * * * * * * * * * * * * * * * * * * *
* Anthony J. Garratt-Reed M.A., D.Phil.
* MIT, Room 13-1027
* 77 Massachusetts Avenue
* Cambridge, MA 02139-4307
* USA
* Phone: (617) 253-4622
* Fax: (617) 258-6478
*





From daemon Mon Dec 11 11:35:30 2000



From: Max Sidorov :      msv-at-nl.feico.com
Date: Mon, 11 Dec 2000 18:27:52 +0100
Subject: TEM - Update again (problems with accessing): Contrast Transfer Function Freeware (ctfExplorer)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear All:

Apparently, Bravenet (who provides a quick link url for me) is down for
updates (at least that's what they say).
The quick link (http://clik.to/ctfexplorer) should start working again
shortly.
In a meantime you can use a direct link: http://www.ctfexplorer.f2s.com

Hope this helps,
Max S

-------------------
Dear All:

ctfExplorer has been updated.
New features/improvements:
1. Now it is possible to export graphs to text (tab-delimited).
2. List-tree with the microscopes is wider now.
3. Tab stops in dialogs arranged in a logical sequence.
4. Now it calculates the Lichte-defocus. (Useful for holography).
5. Additional slider-control is added: allows to change "magnification",
i.e. convergence. Just to show people why it is not always a good idea to
work at 1,000,000x and why some people do high resolution work at as low as
50,000x magnification.

Here is the link: http://clik.to/ctfexplorer (always use this link. it will
direct you to the right location).

Enjoy,

Max Sidorov
---
(until December 31 2000)
TEM Applications Specialist
FEI/Philips Electron Optics
Eindhoven, The Netherlands
e-mail: maxsidorov-at-bigfoot.com (mailto:maxsidorov-at-bigfoot.com)

----------Additional Info----------
Dear All:
I wrote a piece of software which I believe would be of interest to the
microscopy community.
It's a highly interactive program which calculates/displays the contrast
transfer function of TEMs. I know that there are similar programs floating
around but ctfExplorer does not only 1d but also 2d calculations/display
with 2-fold and 3-fold astigmatism imposed. There are other unique features
to it. All parameters (defocus, voltage, Cs, etc) can be changed
interactively.

DISCLAIMER: I do work for FEI/Philips Electron Optics. However, this
software is not an official product of FEI and FEI is not responsible for
its distribution/support. This software is freeware.

To the best of my knowledge, the software does not have any nasty bugs. It
is tested under Windows 95/98 and Windows NT4.

Please give it a try. Please direct your suggestions and comments to
maxsidorov-at-bigfoot.com
I hope you'll find the software useful.

Here is the link: http://clik.to/ctfexplorer

Enjoy,

Max Sidorov
---
TEM Applications Specialist
FEI/Philips Electron Optics
Eindhoven, The Netherlands
e-mail: maxsidorov-at-bigfoot.com






From daemon Mon Dec 11 11:45:24 2000



From: Philip Oshel :      peoshel-at-facstaff.wisc.edu
Date: Mon, 11 Dec 2000 11:41:52 -0600
Subject: MSA Undergraduate Research Scholarship Program

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Microscopy Society of America
Undergraduate Research Scholarship Program

With this year's call for applications the MSA Undergraduate Research
Scholarship Program begins its 13th year providing funding for
undergraduate research. To date over 65 projects covering a wide
range of topics in the physical and biological sciences have received
support through this program. Over the years nearly all the
scholarship recipients have maintained a strong interest in imaging
sciences and have gone on to graduate school, professional school,
teaching, or industry positions.

The program, which is funded by MSA, is able to support approximately
30% to 40% of applicants. The maximal award from MSA is currently
$3000 and helps to provide student stipends, supply costs, and
limited travel expenses associated with the research. Additional
support in the form of instrument use time, equipment purchases, etc.
is generally provided by the studentís supervisor and/or through the
sponsoring institution. Abstracts reporting the research results,
are prepared by scholarship awardees, and published in "Microscopy
and Microanalysis".

The program actively seeks external sources of matching funds in
order to maintain the favorable levels of support both in terms of
the number of projects supported and the level of support for each.
We are extremely grateful for the matching support provided by MSA
sustaining members and individuals. Their support has enabled the
program to increase both the number of awards and the maximum amount
of each award to $3000.

The MSA Undergraduate Research Scholarship Program is currently
soliciting applications from students interested in conducting a
research project which involves the use of any microscopy technique.
Students must be sponsored by a member of MSA. The maximal award is
$3000.

This is not limited to students in the USA or North America. Any
undergraduate student anywhere is eligible to apply for the award,
provided the person supervising their research is a member of MSA.

**The application deadline is Dec 31, 2000.**

Applications can be obtained from the MSA Business Office,
businessoffice-at-msa.microscopy.com or call toll free at (800) 538-3672.

If you have any questions or require additional information regarding
the program please contact either:

Dr. Ralph Albrecht, University of Wisconsin
1656 Linden Drive, Madison, WI 53706
(608) 263-3952 or 263-4162; (608) 262-7420 FAX;
albrecht-at-ahabs.wisc.edu

Dr. Richard Ornberg, Monsanto Company
Analytical Sciences Center, 800 N. Lindbergh Blvd., St. Louis, MO 63167
(314) 694-1184; (314) 694-6727 FAX;
rlornb-at-ccmail.monsanto.com



From daemon Mon Dec 11 12:33:12 2000



From: Paul Anderson :      paanders-at-lynx.dac.neu.edu
Date: Mon, 11 Dec 2000 13:26:12 -0500 (EST)
Subject: JEOL 100CXII

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


FOR SALE:
1988 JEOL 100CXII with (+/-) 45degree tilt stage, dual sample holder,
and Gatan camera system: a rugged, reliable transmission electron
microscope in perfect working order, with water chiller: $30,000.
Buyer is responsible for take-down, shipping, and set-up costs. A Kevex Delta
I Class EDS analysis unit is also included, but currently not installed.

Feel free to come by for a test drive!
317 Egan Research Center
Northeastern University
Boston, MA 02115
For directions and to set up an appointment, call (617) 373-5909.

If interested in purchasing, please contact:
Professor Terry K. Baker
(617) 373-2123





From daemon Mon Dec 11 13:23:55 2000



From: Drew R. Van Orden :      drew-at-rjlg.com
Date: Mon, 11 Dec 2000 14:18:39 -0500
Subject: Please Post - Position Opening

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Position Opening
Materials Scientist

RJ Lee Group's Bay Area Facility, a materials characterization and
consulting laboratory, is seeking a motivated individual to actively manage
projects related to the evaluation of materials and processes used in the
electronics industry. Job applicants should have an MS or equivalent in
physics, materials science or related discipline, at least two years of
relevant work experience, and be amenable to working in a hands-on and
fast-paced team environment. A working knowledge of the application and
theory associated with sample preparation methods and corresponding
analytical techniques such as optical microscopy, scanning electron
microscopy (SEM), transmission electron microscopy (TEM), Auger spectroscopy
and other surface analysis methods is critical. Strong oral and written
communication skills are a must. Job duties would involve, but not be
limited to: materials consulting, developing / evaluating analytical
approaches to constructively investigate problems, and presenting laboratory
results to a variety of technical as well as non-technical personnel.
Salary is commensurate with experience. Interested candidates should
forward qualifications along with salary history to: RJ Lee Group, Inc.,
Attn. Human Resources, 350 Hochberg Road, Monroeville, PA 15146.

RJ Lee Group is an Equal Opportunity Employer



Drew R. Van Orden, PE
RJ Lee Group, Inc.
350 Hochberg Road
Monroeville, PA 15146
(724) 387-1869
drew-at-rjlg.com






From daemon Mon Dec 11 14:43:56 2000



From: Marilyn Howton :      mhowton-at-hsc.wvu.edu
Date: Mon, 11 Dec 2000 15:38:37 -0500
Subject: EM on cell cultures

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am new to this group, so if this is question has been covered, please excuse.
I usually do TEM on tissue from biopsies, etc., and now someone wants EM on cells grown in culture. I have done this for intact monolayers (very tedious), but these would be on trypsined-off and pelleted cells. I need to know if there is an easy way to handle them as an entire pellet, so I don't have to cfg. after each fixation/dehydration step, and so they will be compacted for sectioning.
Thanks in advance for any advice.
Marilyn Howton, Pathology Dept. West Virginia University




From daemon Mon Dec 11 15:49:32 2000



From: Vr. Richard Bejsak-Collorado-Mansfeld :      ricardo-at-ans.com.au
Date: Tue, 12 Dec 2000 08:48:31 +1100
Subject: Mounting media

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear colleagues,

I would like to apologise for my humble trivial request to all highly
qualified expert like you.
I am running website www.coleoptera.org about beetles and I just open new
section FAQ with info about collecting, preparation, techniques etc.

I just like to add section about mounting small insects into permanent
slide.
Is there anything so good as Canadian balsam?

Next question will be how to best prepare insects for SEM..

Any help deeply appreciated.

Keep care and be of good cheer.

Regards

(name) Vratislav Richard Eugene Maria John Baptist
(surname) of Bejsak (Bayshark)-Collorado-Mansfeld

website: http://www.coleoptera.org
listserver: coleoptera on www.egroup.com/group/coleoptera/info.html
Coleoptera - Australia, Tenebrionidae of World
(incl. Lagriinae, Alleculinae)

University of Sydney
The Wentworth Bldg., Box 62
NSW 2006
AUSTRALIA
phone : +61 414 540 465
email: vratislav-at-bigfoot.com
ricardo-at-ans.com.au
(before Ricardo-at-compuserve.com
and ricardo-at-login.cz )

Only after the last tree has been cut down,
only after the last river has been poisoned,
only after the last fish has been caught,
only then will you find that money can not be eaten.'
CREE INDIAN PROPHECY.

Incoming mail is certified Virus Free.
Checked by AVG anti-virus system (http://www.grisoft.com).





From daemon Mon Dec 11 16:03:34 2000



From: alineves-at-unisys.com.br
Date: Mon, 11 Dec 2000 15:59:23 -0600
Subject: Ask-A-Microscopist: Retardation of a specimen in polarized light

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Below is the result of your feedback form. It was submitted by
(alineves-at-unisys.com.br) from
http://www.msa.microscopy.com/Ask-A-Microscopist.html on Monday, December
11, 2000 at 14:50:32
---------------------------------------------------------------------------

Email: alineves-at-unisys.com.br
Name: Aline Neves
School: Federal University of Rio de Janeiro
State: Rio de Janeiro

Question: How do I measure the retardation of a specimen in polarized light
microscopy. I have the Berek conpensator to achive a quantitative measure,
but exactly how do I do it? Where can I get more information about that?

---------------------------------------------------------------------------





From daemon Mon Dec 11 17:03:03 2000



From: sandeep GUPTA :      sundep-at-yahoo.com
Date: Mon, 11 Dec 2000 14:58:16 -0800 (PST)
Subject: Calibration

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Hi
I insect morphology and use Nikon SMZ-2T binocular
stereomicroscope to study the various insect body
parts. I use micrometer inside the ocular lens to
take
measurements. I do not know how to calibrate these
measurements. It will be nice if if someone can refer
literature or material on internet for calibration
and
mathematical calculations.If my question is
incomplete
please let meknow so that i can provide more
nformation.
sandeep gupta


__________________________________________________
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Yahoo! Shopping - Thousands of Stores. Millions of Products.
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From daemon Tue Dec 12 03:00:47 2000



From: Claudia Hayward-Costa :      LS_S562-at-crystal.kingston.ac.uk
Date: Tue, 12 Dec 2000 08:42:07 -0000
Subject: Re: EM on cell cultures

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Dear Marilyn,

I am working with human cell lines in suspension and process the
cells in a pellet.

After the usual problems and much help from this list I worked out
a procedure which works for me.

It is critical that the pellet is small - in my experience not more
than 250 000 cells / pellet. This means you will not or hardly see
the pelleted cells until they have been in OsO4. All the dehydration
steps and infiltration steps are performed on a rotary shaker.

Here it is:

I transfer the cell suspension into conical BEEM capsules and spin
them in home made adapters at 900 x g. (It is essential to use a
swing bucket rotor, otherwise your cells smear along the sides of
the container and float off during the dehydration steps.)

After carefully removing the supernatant with fine tipped mini
pastetts I overlay the pellet with fixative, remove it again with
pastetts and so on. As soon as the OSO4 stains the pellet one
can see it and that makes the handling easier.
I use glass pipetts with OsO4 and propylenoxide and the resin-
propylen mixtures.

I usually infiltrate twice with epon-propylenoxide (1+2 and 1+1
respectively) for 45 minutes, then twice with pure resin. The tips of
glass Pasteur pipetts have to be cut to widen the opening
otherwise it is very difficult to remove the very viscous resin.

For sectioning I carefully flatten the very fine tip of the block with a
razor blade. The cells are evenly distributed, there is no need to cut
away surplus plastic. Just shape the block face to a trapezoid and
it can go straight into the ultramicrotome.

Good luck - if you require more details feel free to ask.

Regards
Claudia

Dr. C. Hayward-Costa
School of Life Sciences
Kingston University
Penrhyn Road, Kingston upon Thames
Surrey KT1 2EE, UK
44(0)208 547 2000 x 2240
Email: c.hayward-at-kingston.ac.uk



From daemon Tue Dec 12 04:26:45 2000



From: Carole Elleman :      c.j.elleman-at-reading.ac.uk
Date: Tue, 12 Dec 2000 10:20:23 -0000
Subject: TEM cell cultures

Contents Retrieved from Microscopy Listserver Archives
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Dear Marylyn Howton - one approach to fixing cells that have been stripped
off is to spin them into a pellet(500rpm) and then take up the pellet in 2%
agar - you can then take the pellet through deydration and embedding and all
the cells remain in a pellet. You can fix the cells in the pellet but that
is rather time consuming and it may be best to do the fixation steps first -
spinning down after each and then make into a pellet.
hope this helps
Carole Elleman




From daemon Tue Dec 12 07:25:21 2000



From: joachim.prutsch-at-leica-microsystems.com
Date: Tue, 12 Dec 2000 14:17:18 +0100
Subject: RE: Wanted Wollaston prisms for Reichert Zetopan DIC scope

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Dear Gordon,


your freind could try to contact Mr. Rasche in Germany:
email: mikrovid-at-gmx.de
homepage: www.mikrovid.com

best wishes, Joachim


Dr. Joachim Prutsch
Product Manager EM Specimen Preparation

Leica Microsystems
Hernalser Hauptstr. 219 email:
Joachim.Prutsch-at-leica-microsystems.com
A-1170 Vienna Tel. +43 1 48899 - 235
Austria Fax +43 1 48899 - 350
---------------------- Weitergeleitet von Joachim
Prutsch/AUVIE/LMSCentral/Leica am 12.12.2000 14:11
---------------------------


"Gordon Couger" {gcouger-at-couger.com} am 09.12.2000 00:12:26



An: microscopy-at-sparc5.microscopy.com

Kopie: (Blindkopie: Joachim
Prutsch/AUVIE/LMSCentral/Leica)



Thema: Wanted Wollaston prisms for Reichert Zetopan DIC
scope






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I have a freind that need then main prism slide containing the Wollaston
prism for Reichert Zetopan DIC scope. This is the slide going in the upper
body of the scope. He also needs the polorizor that fits over the light
soruce but that is of much less importance beause it can be easily made.
While the prism is constructed of unobtainium.

He is willing to pay a reasonable price for the part.

If any of you have a spare or left over you could make a very dissapointed
man a lot happier.

Gordon
Gordon Couger gcouger-at-couger.com
Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00








From daemon Tue Dec 12 07:59:43 2000



From: Ed Calomeni :      ecalomeni-at-mco.edu
Date: Tue, 12 Dec 2000 08:55:23 -0500
Subject: Re: EM on cell cultures

Contents Retrieved from Microscopy Listserver Archives
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Morning Marilyn,

Since you work in a hospital setting, get some unneeded serum from your clinical lab, pellet the sample cells, add 50-100 ul of serum to pellet, mix well
pellet cells again and then overlay with 3% glut. Next day, remove plug of cells/serum, cut into em size tissue pieces and process as you normally would.

Best of Luck,

Ed

Edward P. Calomeni

Medical College of Ohio
Department of Pathology
3000 Arlington Ave.
Toledo, OH 43614

419-383-3484
419-383-3066 (fax)
ecalomeni-at-mco.edu

} } } "Marilyn Howton" {mhowton-at-hsc.wvu.edu} 12/11/00 03:38PM } } }
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I am new to this group, so if this is question has been covered, please excuse.
I usually do TEM on tissue from biopsies, etc., and now someone wants EM on cells grown in culture. I have done this for intact monolayers (very tedious), but these would be on trypsined-off and pelleted cells. I need to know if there is an easy way to handle them as an entire pellet, so I don't have to cfg. after each fixation/dehydration step, and so they will be compacted for sectioning.
Thanks in advance for any advice.
Marilyn Howton, Pathology Dept. West Virginia University






From daemon Tue Dec 12 08:10:25 2000



From: GANTZ-at-med-biophd.bu.edu
Date: Tue, 05 Dec 2000 17:30:12 -0400 (EDT)
Subject: Ammonium Molybdate as Negative Stain

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Dear Nafisa Ghori:
Ammonium Molybdate is especially useful to contrast membranes as in
vesicles or liposomes. It also can be titrated to neutral pH whereas
UA cannot.

Don Gantz
Boston University School of Medicine
Biophysics Dept.



From daemon Tue Dec 12 10:15:22 2000



From: tflore-at-lsuhsc.edu (Flores, Teresa)
Date: Tue, 12 Dec 2000 09:14:36 -0500
Subject: TEM contaminated?

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Colleagues, a pathologist asked to use our Transmission Electron
Microscope(TEM) and when I was going to help him load his copper grids, he
said he would do it as it was a fecal preparation for viral studies. I
found out that material on formar coated copper grids was not fixed. After
he viewed (no photos taken) I pulled the copper grid holder and all items
touched and ultrasonicated with absolute ethanol and then acetone and then
placed items without touching in a 70oC oven for an hour. I feel that is
enough, but would like to know if anyone else has been in this predicament.
Thanks, Teresa





From daemon Tue Dec 12 10:18:41 2000



From: jo verbeeck :      joverbee-at-ruca.ua.ac.be
Date: Tue, 12 Dec 2000 17:05:29 +0000
Subject: TEM image simulation

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I am looking for a computer programm that simulates the contrast induced

by defects in crystals. The defects I am interested in are twins and
steps in the twin planes. Which programms are available in which you
can simulate many different types of defects and that allow to change
the parameters easily?

Best regards,
Jo




From daemon Tue Dec 12 10:40:10 2000



From: Dohnalkova, Alice :      Alice.Dohnalkova-at-pnl.gov
Date: Tue, 12 Dec 2000 08:31:36 -0800
Subject: EM on cell cultures

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Marilyn,
I concentrate the cells by a gentle spin, and embed them in a drop of 2% noble
agar. After hardening and trimming the blocks to a size of you usual biopsy you
can process them without loosing any. A}

Alice Dohnalkova
S&E Assoc., Dep. of Environmental Microbiology
Battelle, Pacific Northwest National Laboratory
MS P7-50
Richland, WA 99352
tel. (509) 372-0692
fax (509) 376-1321


-----Original Message-----
} From: Marilyn Howton [SMTP:mhowton-at-hsc.wvu.edu]
Sent: Monday, December 11, 2000 12:39 PM
To: Microscopy-at-sparc5.microscopy.com


I am new to this group, so if this is question has been covered, please excuse.
I usually do TEM on tissue from biopsies, etc., and now someone wants EM on
cells grown in culture. I have done this for intact monolayers (very tedious),
but these would be on trypsined-off and pelleted cells. I need to know if there
is an easy way to handle them as an entire pellet, so I don't have to cfg. after
each fixation/dehydration step, and so they will be compacted for sectioning.
Thanks in advance for any advice.
Marilyn Howton, Pathology Dept. West Virginia University




From daemon Tue Dec 12 11:10:47 2000



From: reinhard rachel :      reinhard.rachel-at-biologie.uni-regensburg.de
Date: 12 Dec 2000 18:06:37 +0100
Subject: Re: EM on cell cultures

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"Re: EM on cell cultures" (Dec 12, 8:42am)
References: {3A35E4DF.17130.27D219-at-localhost}
X-Mailer: Z-Mail (4.0.1 13Jan97)
To: Claudia Hayward-Costa {LS_S562-at-crystal.kingston.ac.uk}

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I wonder if you have heard of the Hohenberg technique
described some 6 years ago in J.Microscopy; this
technique makes life so much easier for people working with single
cells in suspension, whether they are prokaryotes or eukaryotes.

H.Hohenberg could nicely demonstrate that the embedding and sectioning
is very much facilitated and structures are much better preserved when:
a) cells are taken up in cellulose capillaries, 200 micron in diameter;
b) cells are high-pressure frozen (as the very first step
for cryo-immobilization)
c) cells are freeze-substituted but not dehydrated at RT.
This technique has been used in the meanwhile by many other
people with success, ...
but still is not known to all working with single cells
in suspensions.

Hohenberg et al (1994) J. Microscopy 175, 34-43

have fun!
Reinhard Rachel

Dr. Reinhard Rachel
Universitaet Regensburg
Lehrstuhl fuer Mikrobiologie (Prof. Dr. K.O. Stetter)
D - 93040 Regensburg
Tel.: +49-941-943-4534
Fax.: +49-941-943-1824
e-mail: Reinhard.Rachel-at-biologie.uni-r.de



From daemon Tue Dec 12 12:15:53 2000



From: Carole Elleman :      c.j.elleman-at-reading.ac.uk
Date: Tue, 12 Dec 2000 10:20:23 -0000
Subject: TEM cell cultures

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Dear Marylyn Howton - one approach to fixing cells that have been stripped
off is to spin them into a pellet(500rpm) and then take up the pellet in 2%
agar - you can then take the pellet through deydration and embedding and all
the cells remain in a pellet. You can fix the cells in the pellet but that
is rather time consuming and it may be best to do the fixation steps first -
spinning down after each and then make into a pellet.
hope this helps
Carole Elleman







From daemon Tue Dec 12 12:23:33 2000



From: Ed Calomeni :      ecalomeni-at-mco.edu
Date: Tue, 12 Dec 2000 08:55:23 -0500
Subject: Re: EM on cell cultures

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Morning Marilyn,

Since you work in a hospital setting, get some unneeded serum from your clinical lab, pellet the sample cells, add 50-100 ul of serum to pellet, mix well
pellet cells again and then overlay with 3% glut. Next day, remove plug of cells/serum, cut into em size tissue pieces and process as you normally would.

Best of Luck,

Ed

Edward P. Calomeni

Medical College of Ohio
Department of Pathology
3000 Arlington Ave.
Toledo, OH 43614

419-383-3484
419-383-3066 (fax)
ecalomeni-at-mco.edu

} } } "Marilyn Howton" {mhowton-at-hsc.wvu.edu} 12/11/00 03:38PM } } }
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I am new to this group, so if this is question has been covered, please excuse.
I usually do TEM on tissue from biopsies, etc., and now someone wants EM on cells grown in culture. I have done this for intact monolayers (very tedious), but these would be on trypsined-off and pelleted cells. I need to know if there is an easy way to handle them as an entire pellet, so I don't have to cfg. after each fixation/dehydration step, and so they will be compacted for sectioning.
Thanks in advance for any advice.
Marilyn Howton, Pathology Dept. West Virginia University









From daemon Tue Dec 12 12:30:24 2000



From: sghoshro-at-nmsu.edu
Date: Tue, 12 Dec 2000 11:25:21 -0700 (MST)
Subject: EM on cell culture

Contents Retrieved from Microscopy Listserver Archives
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Marilyn,

You can use a tabletop airfuge with a very small rotor. Each tube holds
about 0.2 ml liquid. You can spin your sample for about an hour at a very
high speed and it will give you a very tight pellet. I used to spin the
sample in fixative and once you are done spinning, you can process the
pellet and do osmication, dehydration, infiltration and embedding.

Good luck,

Soumitra


*************************************************************
Soumitra Ghoshroy Ph.D.
Electron Microscopy Lab and Fluorescence Imaging Facility
Department of Biology
Box 3EML
New Mexico State University
Las Cruces, NM 88003
Tel: 505-646-3600
Fax: 505-646-5665
e-mail:sghoshro-at-nmsu.edu
http://confocal.nmsu.edu/eml





From daemon Tue Dec 12 12:46:34 2000



From: Timothy Schneider :      Timothy.Schneider-at-Mail.TJU.EDU
Date: Tue, 12 Dec 2000 13:34:25 -0500
Subject: PROCESSING CELL SUSPENSIONS

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Hi:

I have been working with human red blood cells grown in cell culture that
are infected with Plasmodium falciparum for the past 9 years. The way I
handle them is to use normal fixatives (glut, OsO4, uranyl acetate) while
they are in suspensions and spin them down in-between steps. Following
fixation I spin them down in warm (45 degree C) 3% agarose (Sigma A5030
25gm, type 9 ultra low gelling temperature). When the agarose cools to room
temperature the pellet becomes like Jell-O in the tip of the 15ml falcon
tube. Then I simply cut off the tip and transfer the "glued together
pellet" to a glass vial and continue with dehydration and infiltration steps
as if I was handling a small hunk of tissue. Good luck, Tim

Timothy G. Schneider
Director of Electron Microscopy
Department of Pathology
Room 229 Jefferson Hall
Thomas Jefferson University
1020 Locust St.
Philadelphia Pa. 19107
215-503-4798 work
610-613-8170 cellular
timothy.schneider-at-mail.tju.edu




From daemon Tue Dec 12 12:51:47 2000



From: DCiaburri-at-gdds.com
Date: Tue, 12 Dec 2000 13:44:03 -0500
Subject: RE: Wanted Wollaston prisms for Reichert Zetopan DIC scope

Contents Retrieved from Microscopy Listserver Archives
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Gordon,

Another person to try would be Dave Paschke, The Dawson Co.,
(617)484-7900. He had some parts for our Reichert MeF2 when I spoke to
him a year ago...you never know.

Diane Ciaburri
General Dynamics
Pittsfield, MA 01235
(413)494-2847





"joachim.prutsch-at-leica-microsystems.com"
12/12/00 08:17 AM


To: Microscopy-at-sparc5.microscopy.com
cc:
Subject: RE: Wanted Wollaston prisms for Reichert Zetopan DIC scope


Dear Gordon,


your freind could try to contact Mr. Rasche in Germany:
email: mikrovid-at-gmx.de
homepage: www.mikrovid.com

best wishes, Joachim


Dr. Joachim Prutsch
Product Manager EM Specimen Preparation

Leica Microsystems
Hernalser Hauptstr. 219 email:
Joachim.Prutsch-at-leica-microsystems.com
A-1170 Vienna Tel. +43 1 48899 - 235
Austria Fax +43 1 48899 - 350
---------------------- Weitergeleitet von Joachim
Prutsch/AUVIE/LMSCentral/Leica am 12.12.2000 14:11
---------------------------


"Gordon Couger" {gcouger-at-couger.com} am 09.12.2000 00:12:26



An: microscopy-at-sparc5.microscopy.com

Kopie: (Blindkopie: Joachim
Prutsch/AUVIE/LMSCentral/Leica)



Thema: Wanted Wollaston prisms for Reichert Zetopan DIC
scope


I have a freind that need then main prism slide containing the Wollaston
prism for Reichert Zetopan DIC scope. This is the slide going in the upper
body of the scope. He also needs the polorizor that fits over the light
soruce but that is of much less importance beause it can be easily made.
While the prism is constructed of unobtainium.

He is willing to pay a reasonable price for the part.

If any of you have a spare or left over you could make a very dissapointed
man a lot happier.

Gordon
Gordon Couger gcouger-at-couger.com
Stillwater, OK www.couger.com/gcouger
405 624-2855 GMT -6:00












From daemon Tue Dec 12 12:57:07 2000



From: Paul Webster :      pwebster-at-mailhouse.hei.org
Date: 12 Dec 00 11:01:35 -0800
Subject: RE: EM on cell cultures

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From: Paul Webster :      pwebster-at-mailhouse.hei.org
Date: 12 Dec 00 11:01:35 -0800
Subject: RE: EM on cell cultures

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Reply to: RE: EM on cell cultures
Dear Marilyn,

A good way to prepare cell monolayers for EM by embedding them as a pellet is as follows:

Fix the cells on the substrate by adding double strength fixative to the culture medium. This will contain serum which is good for the next steps. Leave the fixative on the cells for about 2-5 min and then carefully scrape off the monolayer using a small scraper specially made for this from either a small piece of teflon or a soft wooden stick previously soaked in buffer. The cells will come off the substrate in either a single monolayer (if there are tight junctions present, or as a powdery suspension.
Quickly take the detached cells from the dish and centrifuge (I use full speed on the benchtop Eppendorf for about 2 seconds to get a pellet). If you do this without protein being present in the fixative (eg serum) the cells will not pellet but will stick to the sides of the tube (smearing). Once the cells have pelleted DO NOT resuspend them. Remove the fixative and medium and replace it with fresh fixative. Leave for the amount of time you want to fix for (eg 1hr). You will find that after the fixation is complete you will be able to remove the pellet from the bottom of the tube and treat it as if it was a piece of tissue. You should be able to cut it into smaller pieces for subsequent embedding. Follow your favorite protocol for this. I am sure that you will have pellets that do not fall apart. The secret is to fix as a pellet a.s.a.p. and then do not disturb until after fixation is complete. I think the fixative cross-links extracellular components of the cells together but this only happens if the cells are in close proximity as fixation occurs.

If you do prefer to process in the tube they were pelleted in, then a neat trick for speeding up all the steps is to centrifuge after each change of solution. You will see how quickly things go into the pellet if you centrifuge in the presence of osmium tetroxide. A 2 min centrifugation at top speed will cause even a large pellet to turn black all the way through. If you continue the processing in this tube then make sure the final infiltration in the resin is sufficient to go allthe way through the pellet. Be careful to remove all dehydrating solutions and propylene oxide from the tube. I tip them upside down for about half an hour before adding the final resin changes. You will soon realize if you did this successfully - a soft pellet will be the result of too much proylene oxide in the resin. I prefer to embed them after cutting into small pieces.

As they are a pellet of individual cells they will be much easier to embed in resin than a solid tissue piece. Any poor morphology you get will probably not be due to what you do them but what happens before you put them in fixative. Trypsin is not good for morphology, neither is transfection. Ccentrifugation or scraping prior to fixation will affect morphology too. It is a good rule to physically handle the cells as little as possible prior to fixation.

The biggest surprize is that they will have poor morphology if they have only been left to grow fror a day or two after passage. It is only after about three days that they really start to recover from this. Unfortunately, they will look really good in the light microscope so poor morphology will be your problem!

I hope this helps.

Paul Webster.


Paul Webster, Ph.D
House Ear Institute
2100 West Third Street
Los Angeles, CA 90057
phone:213 273 8026
fax: 213 413 6739
e-mail: pwebster-at-hei.org
http://www.hei.org/htm/aemi.htm

Marilyn Howton wrote:
} ------------------------------------------------------------------------
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} -----------------------------------------------------------------------.
}
}
} I am new to this group, so if this is question has been covered, please excuse.
} I usually do TEM on tissue from biopsies, etc., and now someone wants EM on } cells grown in culture. I have done this for intact monolayers (very tedious), } but these would be on trypsined-off and pelleted cells. I need to know if there } is an easy way to handle them as an entire pellet, so I don't have to cfg. after each } fixation/dehydration step, and so they will be compacted for sectioning.
} Thanks in advance for any advice. } Marilyn Howton, Pathology Dept. West Virginia University
}
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From daemon Tue Dec 12 13:59:29 2000



From: Lehman, Ann :      Ann.Lehman-at-trincoll.edu
Date: Tue, 12 Dec 2000 14:56:35 -0500
Subject: Rotary shadowing

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Dear Listers,

Can anyone suggest a good reference or share a technique for preparing C-Pt
rotary shadowed collagen molecules on mica for TEM?

Thanks.

----------------
Ann Hein Lehman
EM Facility Manager
Trinity College
Hartford, CT 06106
v. 860-297-4289
f. 860-297-2538
e. ann.lehman-at-trincoll.edu



From daemon Tue Dec 12 14:22:37 2000



From: Claudia Hayward-Costa :      LS_S562-at-crystal.kingston.ac.uk
Date: Tue, 12 Dec 2000 08:42:07 -0000
Subject: Re: EM on cell cultures

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------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


Dear Marilyn,

I am working with human cell lines in suspension and process the
cells in a pellet.

After the usual problems and much help from this list I worked out
a procedure which works for me.

It is critical that the pellet is small - in my experience not more
than 250 000 cells / pellet. This means you will not or hardly see
the pelleted cells until they have been in OsO4. All the dehydration
steps and infiltration steps are performed on a rotary shaker.

Here it is:

I transfer the cell suspension into conical BEEM capsules and spin
them in home made adapters at 900 x g. (It is essential to use a
swing bucket rotor, otherwise your cells smear along the sides of
the container and float off during the dehydration steps.)

After carefully removing the supernatant with fine tipped mini
pastetts I overlay the pellet with fixative, remove it again with
pastetts and so on. As soon as the OSO4 stains the pellet one
can see it and that makes the handling easier.
I use glass pipetts with OsO4 and propylenoxide and the resin-
propylen mixtures.

I usually infiltrate twice with epon-propylenoxide (1+2 and 1+1
respectively) for 45 minutes, then twice with pure resin. The tips of
glass Pasteur pipetts have to be cut to widen the opening
otherwise it is very difficult to remove the very viscous resin.

For sectioning I carefully flatten the very fine tip of the block with a
razor blade. The cells are evenly distributed, there is no need to cut
away surplus plastic. Just shape the block face to a trapezoid and
it can go straight into the ultramicrotome.

Good luck - if you require more details feel free to ask.

Regards
Claudia

Dr. C. Hayward-Costa
School of Life Sciences
Kingston University
Penrhyn Road, Kingston upon Thames
Surrey KT1 2EE, UK
44(0)208 547 2000 x 2240
Email: c.hayward-at-kingston.ac.uk






From daemon Tue Dec 12 14:58:03 2000



From: Owen P. Mills :      opmills-at-mtu.edu
Date: Tue, 12 Dec 2000 15:55:13 -0500
Subject: Jena microscope bulb

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Can anyone tell us where to buy a 12V 50W bulb part number HLWS5-A (by
Narva) for Jena scope. Please respond to me directly at the address below.

Thanks

Owen P. Mills
Electron Optics Facility Engineer
Michigan Technological University
Department of Materials Science and Engineering
Rm 512 M&M Building
Houghton, Mi 49931
Ph: 906-487-2002
FAX: 906-487-2934
Email: opmills-at-mtu.edu
URL: http://www.mm.mtu.edu/~opmills/index.html




From daemon Tue Dec 12 15:05:12 2000



From: Paula Sicurello :      patpxs-at-gwumc.edu
Date: Tue, 12 Dec 2000 16:06:07 -0500
Subject: Summary

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Hi Everybody,

Here's a summary of the advice I received regarding my falling off section problem. It's long but read the last suggestion, it's my favorite.

Formvar coating the tradition way, or dipping the grid into formvar or 0.01% BSA. Dry by dragging grid across filter paper.
Bake the sections on to the grid-use either a 60 or 35 degree C oven overnight.
Heat fixing the sections using either a hot plate or slide warmer for 10 minutes.
Use Coat-Quick G.
Flame the grids with an alcohol lamp until the grid just changes color.
When working with Spurr's resin, use Veco grids not Gilder.
Make sure the water is utra-clean. Use double distilled, deionized, or microfiltered.
Various cleaning techniques:
1. 100% acetone wash.
2. 0.1M Nitric acid dip, rinse with dd water, dry on filter paper.
3. 50% acetic acid, 50% acetic acid, 100% acetone-dip grid into each solution then dry well before use.
4. 40 ml concentrated HCL in 400 ml dd water, add 40 ml acetone, take volume to 500 ml with dd water.
Sonicate grids in 50ml beaker for 1-2 minutes, discard solution and rinse 2 X with acetone (30 seconds each)
dry on filter paper. Do this every day to prevent oxidation from building up on the grids.
5. 100ml clean beaker, cover grids with 20ml glacial acetic acid, sonicate for 5-10 minutes, rinse with 100% acetone
until the smell of acetic acid is gone, rinse with 100% ethanol, pipette off the excess 100% ethanol, invert beaker
onto filter paper in a glass petri dish. Place in a 60 degree C oven for about 60 minutes, the grids will fall to the
bottom of the petri dish, cover the dish with the glass top, the grids will be good for a while. Glass is used to
avoid the static that plastic can have.
Put sections on the dull side.

Last but not least......

get a new job or get a technician.

I apologize for this being so lengthy, but I thought others might benefit from this information.



Paula :-)


Paula Sicurello
George Washington Univ. Medical Center
Dept. of Pathology, Ross Hall rm 505
Electron Microscope Lab
2300 Eye St.
Washington, DC 20037
202-994-2930 phone
202-994-2518 fax

Paula Sicurello
George Washington Univ. Medical Center
Dept. of Pathology, Ross Hall rm 505
Electron Microscope Lab
2300 Eye St.
Washington, DC 20037
202-994-2930 phone
202-994-2518 fax

Paula Sicurello
George Washington Univ. Medical Center
Dept. of Pathology, Ross Hall rm 505
Electron Microscope Lab
2300 Eye St.
Washington, DC 20037
202-994-2930 phone
202-994-2518 fax





From daemon Tue Dec 12 17:26:21 2000



From: francine.gauthier-at-mcc.gouv.qc.ca ()
Date: Tue, 12 Dec 2000 17:20:46 -0600
Subject: Ask-A-Microscopist: identify types of starch

Contents Retrieved from Microscopy Listserver Archives
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Colleagues...

Can anyone answer this question . Remember
Ask-A-Microscopist is a forum that is used by NON-MICROSCOPISTS
looking for help so keep try to your technical details at the right level.

Nestor

---------------------------------------------------------------------------

Email: francine.gauthier-at-mcc.gouv.qc.ca
Name: Francine Gauthier

Question: Is it possible to identify types of starch glue (rice, wheat,
corn, potato, etc)under microscope? Is there any visuals available
somewhere for comparisons?

---------------------------------------------------------------------------





From daemon Wed Dec 13 03:39:35 2000



From: GuessWho :      olivier.balmes-at-oorg2.lth.se
Date: Wed, 13 Dec 2000 10:32:32 +0100
Subject: TEM grid for plunging of organic solvents

Contents Retrieved from Microscopy Listserver Archives
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Dear list members,

We are trying to do some cryo TEM of organic solvents based samples, and
the
grids we have used so far were not very satisfactory, some solvents
obviously
influenced the formvar (and maybe the carbon ?) of the grid making it
beam
sensitive.
We are planning on purchasing another type of grid (pure carbon lacey
film,
silicon dioxide or maybe silicon nitride) and I would like to know if
anybody
has tried anything for that kind of application.
Any suggestion or comment most welcome !

O.Balmes








From daemon Wed Dec 13 04:49:08 2000



From: Faerber Jacques :      Jacques.Faerber-at-ipcms.u-strasbg.fr
Date: Wed, 13 Dec 2000 11:41:46 +0100
Subject: S4700 and JSM 6700F (fwd)

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Dear colegues

Thanks to all how ansered my questions about these two FE-SEM. Its a pitty
that I heard about MSA Listserver only two weeks ago. It would have been
interesting to become these informations a bit sooner. It was very
fruitfull to have some return of experience and offline discussions with
some. Amazing was that amost only Hitachi owners had something to tell.
And they are all pleased about their instrument. True that the Jeol is
new, and much different from the former.

We have seen that our conclusions are similar that a lot of others, and so
in a few words, what we can say about these two SEMs:

The Jeol 6700F seems to have at low primery energy (1-2 keV) and
short working distance (1-3 mm) the best resolution and the easiest way to
become nice images. Charging effect are weak, and magnetic samples have
no visible propensity to "fly" in the chamber (magnetic nano powder, for
exemple) ! The limitation now, is that this good resolution is lost very
fast with increasing the working distance (6-8 mm). And the BSE working
distance is 6-8 mm, analytical working distance 15 mm. Shure that in BSE
or in X ray microanalysis, higher energy will bee used. But...and tilt ?
And the vacuum chamber is limited in avaible ports.

On the other hand, the Hitachi may have the capacity to do so
good, but it needs more experience of the manipulator and more time.
One is shure : the "In Lens" detector of the Hitachi works well (is
probably the best) at short and long working distances, gives a realy
surface information, and with the last improvement, gives the possibility
of composition contrast at low energy (schould be less sensitif to charge
effects too, but we didn't be convinced).

So, mesured with a ladle (do you say so in english ? "Mesurer à la
louche", in french !) The Jeol seems to be best for ultra high resolution
at low energy and short working distance. The Hitachi seems to be best if
you want to have the best images possible at analytical distance (12 mm),
backscatered detector on place and the two "secondary" dectectors
together. And is also capable to reach ultra high resolution. We didn't
make detailed tests at high energy (} 15 keV). All instruments seems to be
comparable at high energy, and then you don't observe the real surface.

But, and now I'll conclued, the two apparatus are VERY good, and it is
VERY difficult to make a choice. If you have monney, buy one of eatch ! We
have choiced the Jeol one, but whith some regree for Hitachi's "In Lens"
detector, versatility and reliability.

I've said nothing about Leo's 1500 serie and Philips XL30-S-FEG, witch are
also interresting. We have test them, but it would be to long to speak
about these now. But cold FEG seems to give better high resolutions
images,and is to be prefered if you don't need the long term stability of
Shottky FE, for intensive XRay microanalysis fot exemple. Gemini's optical
concept is very interresting, but more "classical" design give as good
results.

I'm still intersted on other opinions or test results, and also avaible
for questions, true MSA list server or on my direct mail.

By and thanks to all


J. Faerber
IPCMS-GSI
23, rue de Loess
67037 Strasbourg CEDEX

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)0 88 10 72 48
E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr





From daemon Wed Dec 13 06:19:55 2000



From: olivier.balmes-at-oorg2.lth.se :      olivier.balmes-at-oorg2.lth.se
Date: Wed, 13 Dec 2000 13:12:01 +0100
Subject: TEM grids for plunging of organic solvents

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Dear list members,

We are trying to do some cryo TEM of organic solvents based samples, and
the
grids we have used so far were not very satisfactory, some solvents
obviously
influenced the formvar (and maybe the carbon ?) of the grid making it
beam
sensitive.
We are planning on purchasing another type of grid (pure carbon lacey
film,
silicon dioxide or maybe silicon nitride) and I would like to know if
anybody
has tried anything for that kind of application.
Any suggestion or comment most welcome !

O.Balmes








From daemon Wed Dec 13 07:28:27 2000



From: DrJohnRuss-at-aol.com
Date: Wed, 13 Dec 2000 08:19:25 EST
Subject: Practical Stereology textbook

Contents Retrieved from Microscopy Listserver Archives
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subject: J. C. Russ, R. T. Dehoff, Practical Stereology, 2nd Edition, Plenum
Press, New York, 2000 (!?!), isbn 0-306-46476-4

Some folks on this list have an early draft of my forthcoming (more on that
below!) book on stereology, which was distributed on the CD with the Image
Processing Tool Kit and also handed out in notes form at the various image
analysis workshops that I’ve taught for the past many years. Bob Dehoff (who
teaches those workshops with me) and I completed the final version of that
book in December, 1999, and sent it in to the publisher. It has taken them a
very long time to get around to doing anything with the book, and they are
still apparently having a lot of internal technical problems (not worth going
into here - but *.doc files and *.tif files on a CD seem to be a little too
high tech for these people, and they clearly don’t have any idea what a *.pdf
file is good for). At the M&M meeting in Philadelphia, quite a few people
asked me about the book and at that time Plenum was promising that it would
be available in September. Well, of course, it wasn’t, and still isn’t and
frankly I’m getting tired of apologizing for it. None of the delay has
anything to do with the authors, it is strictly production difficulties and a
high level of technical incompetence at the publisher’s end. Anyway, to make
a long story less long, I’ve put a copy of the entire book on the web in the
form of a pdf file. In order to keep the download times reasonable (it is
still 7.1 MB) the graphics are jpeg compressed, but I think still useful and
legible. Anyone who wants to download a copy is welcome to it. When the book
eventually (!) comes out the on-line version will evaporate. You may want to
purchase a copy to get the index and somewhat better graphics, but I will
leave that to your conscience. For the present, you can find a link to it at
{http://members.aol.com/ImagProcTK/updates} (look toward the bottom of the
page under "Data Analysis") or on my web page
{http://members.aol.com/DrJohnRuss} (go to the "links" section). In the
on-line file, the contents are linked to the book pages and of course you can
use Acrobat Reader to search for any word in the text.

John Russ



From daemon Wed Dec 13 07:54:22 2000



From: Walling, Dennis :      dwalling-at-utmb.edu
Date: Wed, 13 Dec 2000 07:48:39 -0600
Subject: Tissue staining question

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} I am looking for a tissue histology stain for human tongue
} epithelium for use with tetramethylrhodamine-labeled probes. The stain
} must not
} fluoresce under 546nm wavelength light and also must not quench
} tetramethylrhodamine fluorescence. Haematoxylin, eosin, and methylene
} blue each
} fail to meet these requirements. Does anyone have any suggestions?
}
} Dennis M. Walling, M.D.
} Assistant Professor
} Division of Infectious Diseases
} Department of Internal Medicine
} The University of Texas Medical Branch
} 301 University Boulevard
} Galveston, TX 77555-0835
}
} Telephone: (409) 747-2361
} Fax: (409) 772-6527
} E-mail: dwalling-at-utmb.edu
}





From daemon Wed Dec 13 08:18:26 2000



From: Rozeveld, Steve (SJ) :      SJROZEVELD-at-dow.com
Date: Wed, 13 Dec 2000 09:10:01 -0500
Subject: open position: Particle analyst- Light microscopy

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POSITION OPEN

The Materials Characterization Group in Dow’s Corporate R&D Analytical
Science laboratory has a full-time professional level opening for a particle
analyst/light microscopist at Dow’s facility in Midland, MI. The primary
responsibilities include working with partners to support research and
production projects across a broad range of products. Good written and oral
communication skills and the ability to work both independently and in a
team environment are extremely important.

Key responsibilities will include:
* Strong problem solving skills
* Operation of particle size characterization instrumentation
* Operation of light microscopes and supporting image analysis
software packages
* Method development
* Documentation of work
* Compliance with safety and quality programs

Qualifications:
* A candidate with a BS or MS degree in material science, chemistry,
or physics with experience in light microscopy and particle size
characterization.
* Experience with common commercially available particle size
measurement instrumentation - single-particle, ensemble and fractionation
based.
* Experience in the general area of polarized light microscopy,
digital image acquisition, and quantitative image analysis.

Please e-mail or send your resume and cover letter, with reference to this
ad to: Email: {R&D-at-Dow.com} or The Dow Chemical Company, Employee
Development Center, Workforce Planning, Midland, MI 48674. Email
respondents must list Job 00622LUSA/JMK-JB and their last name as the first
and second items on the subject line. Only those selected for an interview
will be contacted.

We are an equal opportunity employer and offer a competitive compensation
and benefits package including 401k, stock purchase, tuition reimbursement
and performance incentives. The Dow Chemical Company is the fifth largest
chemical company in the world with annual sales of US$20 billion. Dow
manufactures and supplies chemicals, plastics, and agricultural products for
customers in 164 countries and employs approximately 43,000 people
worldwide. For more news and information about Dow, visit our web site at
www.dow.com.




From daemon Wed Dec 13 08:18:26 2000



From: Rozeveld, Steve (SJ) :      SJROZEVELD-at-dow.com
Date: Wed, 13 Dec 2000 08:10:13 -0600
Subject: open position: Polymer Microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Polymer Microscopy

Company: The Dow Chemical Company

Location: Midland, Michigan

Qualifications (education, certification, language, etc.) and experience
required:

A candidate with a MS or Ph.D. degree in polymer science, material science
or chemistry is preferred with prior experience in electron microscopy.
Good written and oral communication skills and the ability to work both
independently and in a team environment are extremely important.

Job Overview:

The Surface/Microscopy/X-ray (SMX) Group in Dow's Corporate R&D
Analytical Science Laboratory has a professional level full time opening for
a polymer microscopist. The primary responsibilities include working with
partners to support research projects involving new and existing products in
Dow's polymer businesses.

Key responsibilities will include:

1. Extensive problem solving.
2. Microscopy preparation technique experience including
3. Ultramicrotomy and cryo-ultramicrotomy.
4. Operation of light microscopes, scanning and transmission electron
microscopes.
5. Interpretation of images.
6. Documentation of work.
7. Compliance with safety and quality programs.
8. Active member of project and SMX work teams.

We are an equal opportunity employer and offer a competitive compensation
and benefits package including 401k, stock purchase, tuition reimbursement
and performance incentives.
Please e-mail or send your resume and cover letter, with reference to this
ad to:
Email: {R&D-at-Dow.com} or The Dow Chemical Company, Employee Development
Center,
Workforce Planning, Midland, MI 48674.
Email respondents must list Job 00623LUSA/JMK-JB and their last name as the
first and second items on the subject line.

Only those selected for an interview will be contacted.






From daemon Wed Dec 13 08:21:55 2000



From: SMancuso-at-specialmetals.com
Date: Wed, 13 Dec 2000 09:18:34 -0500
Subject: Remote SEM

Contents Retrieved from Microscopy Listserver Archives
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Can anyone tell me where I can find information on remote SEM?
Is it possible to install remote SEM capabilities on an older existing
instrument (JEOL 35C)?
Does this technology work for LOM also?


Sam O. Mancuso
Special Metals Corporation
Research & Development
New Hartford, NY 13413
smancuso-at-specialmetals.com





From daemon Wed Dec 13 09:03:29 2000



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Wed, 13 Dec 2000 08:56:16 -0600
Subject: Ask-A-Microscopist: Starch grains

Contents Retrieved from Microscopy Listserver Archives
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Francine,

In my opinion, light microscopy is probably most useful in differentiating
starch grains, although SEM is sometimes used.

By using a light microscope with two polarizing filters, it is possible to
view a "cross" or "x-shaped" pattern within the starch grains. (This has
sometimes been referred to as a "Maltese Cross".) The appearance of this
cross can be an indicator of starch type. Also, by rotating one of the
polarizers you can observe the cross as it comes into view, rotates a bit,
then disappears again. Measuring the rotation arc of the polarizer within
which the cross is visible gives you another criterion.

Other ways of telling starches apart are, of course, their size and shape.
Sizes should be determined by averaging a number of grains, since they can
be quite variable---in fact, the amount of variation in size is yet another
criterion. Shapes range from round to various types of ovals and irregular
ovoids. SEM excels at this, allowing very precise measurements.

If you really want to get involved, it's possible to set up heated water
baths and sample a slurry of starch grains as the temperature rises, then
count the melted versus unmelted grains in the light microscope. Melted
starch grains are amoeba-like blobs, instead of nicely defined shapes.
Different starches have different melting characteristics.

There is a two-volume set of work by Edward Tyson Reichert (1913), named
"The Differentiation and Specificity of Starches in Relation to Genera,
Species, Etc." It is loaded with methods and photos.

Here is a web site you may want to look at:
http://www.siu.edu/~ebl/amylose.htm. I used to do starch research in the
lab that puts this page out, back in "the old days".

Hope this gets you started. Good luck.

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/






From daemon Wed Dec 13 09:14:10 2000



From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Wed, 13 Dec 2000 09:09:25 -0600
Subject: Re: Remote SEM: TelePresence Tutorial at M&M 2000

Contents Retrieved from Microscopy Listserver Archives
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} -----cut & paste-------- {
} Can anyone tell me where I can find information on remote SEM?
} Sam O. Mancuso

===================================================

You will need to carefully define what you mean by "Remote SEM".
Do you mean true TelePresence or just TeleObservation.

You should begin by looking at the Microscopy and Microanalysis
Proceedings for
1995, 1996, 1998, and 2000 there are a number of full session as well
as articles in there by the various groups in the world working in this area.

Viewing images on-line is do-able in virtually any imaging system, with
sufficient hardware upgrades, but remote "TeleOperation" can be a much
more involved proposition particuliarly for an older instruments.

You might consider getting a copy of the MSA Education Committee Video Tapes
made during the Tutorials presented at the Microscopy and Microanalysis 2000
meeting.

http://www.msa.microscopy.com/MSADocs/MSAVideotapeCatalogue95.html

I did a presentation therein called Microscopy and Microanalysis over the
Internet,
which discussed a pretty full range of options. I don't know if 2000
meeting videos
are ready yet, but you can contact Greg Erdos for that information at the
above
WWW page.


There are a number of vendors which can interface older instruments
to computer systems for image acquisition which the minimum starting point.
This can then, in principle, be adapted to TeleObservation. You should
talk to
the following companies: 4Pi, Quartz, Gatan , EmiSpec (to name a few
there are more
so please check the exhibitors list from the M&M 2000 meeting)
as well as literally any company that manufacturers EDS equipment (a long
list....).
Most of the Electron Optical manufacturers also offer or will soon offer
this as options on
their newer instruments but in all likelihood for your older instrument
you will have to go to the accessory market arena.

There is also going to be a session at the Microscopy & Microanalysis
Technologist's Forum at the 2001 Meeting which will discuss the
issue of TelePresence, you may wish to attend that meeting in Long Beach
August 5-9, 2001.
http://www.microscopy.com/MSAMeetings/MM01/MMHomePage.html

Nestor
Your Friendly Neighborhood SysOp

Disclaimer: I have no commerical interests in the companies mentioned
herein, but you might say I have a mild vested interest in "TelePresence" and
it's application to Research and Education ;-)

As always my TPM Lab is open to the world.... http://tpm.amc.anl.gov.
your welcome to visit anytime...







From daemon Wed Dec 13 11:12:29 2000



From: Thearith H. Ung :      tung-at-qdots.com
Date: Wed, 13 Dec 2000 09:04:31 -0800
Subject: TEM Course

Contents Retrieved from Microscopy Listserver Archives
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Dear all,

Does anyone know of any good TEM courses? Thank you.

Regards,
Thearith


_________________________

Thearith Ung, Ph.D.
Quantum Dot Corporation
26136 Research Road
Hayward CA 94545
Tel: (510)-887-8775 (x4125)
Fax:(510)-783-9729
www.qdots.com




From daemon Wed Dec 13 12:44:15 2000



From: emxray-at-uwindsor.ca
Date: Wed, 13 Dec 2000 13:35:33 -0500
Subject: Simard Company

Contents Retrieved from Microscopy Listserver Archives
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J. W. Robinson
Mechanical, Automotive, and Materials Engineering
University of Windsor
Windsor, Ontario
N9B 3P4

Phone : 519-253-4232, ext 2598

Could anyone send me any information on the L. M. Simard company,
maker of the tri-mag sputtering source? I need to order replacement parts
and the last phone number I have for them is no longer in service. Any
help will be appreciated.

Thank you.

John




From daemon Wed Dec 13 12:44:20 2000



From: DrJohnRuss-at-aol.com
Date: Wed, 13 Dec 2000 13:37:31 EST
Subject: wrong link for stereology book...

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ooops - guess I need to type more carefully. The correct URL for downloading
the Practical Stereology textbook is
{http://members.aol.com/ImagProcTK/update.html} . The link there goes to a
grad student's computer (someone who had enough storage space that I could
use for a while), and from time to time that machine may go off line (when he
is doing some "real" work), so if you don't get through at first be patient
and try again in a few hours or the next day.

Sorry for the hassle

John Russ




From daemon Wed Dec 13 14:51:03 2000



From: Ping Li :      pli-at-is.dal.ca
Date: Wed, 13 Dec 2000 16:45:09 -0400
Subject: Re: TEM user fee

Contents Retrieved from Microscopy Listserver Archives
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We just installed a Tecnai 12. I am not sure what is a reasonable charge for
users to use the TEM. We are at university and a self-supported EM suite.
Could you please let me know your opinion or the user fee (hourly rate)
charged at your work place? especially at Canadian universities.

Thank you.
Ping




From daemon Wed Dec 13 15:08:46 2000



From: Larry Stoter :      larry-at-care4free.net
Date: Wed, 13 Dec 2000 20:17:06 +0000
Subject: Re: Remote SEM

Contents Retrieved from Microscopy Listserver Archives
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} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

There is a freeware/shareware application WinVNC which basically
allows remote control over a network of any other PC and the
applications running on that PC. While not a perfect solution, it
works well running PC-driven SEMs, such as the JEOL 5500/5600/5900.
We have experimented with it in the JEOL UK Application Lab and,
considering it is 'free', are happy with it.

Addressing your specific problem, you would first have to convert
your JSM-35 to a PC driven SEM. I don't know about the US but in the
UK, there are several 3rd party companies offering such conversions.
If you don't get any information via the list, contact JEOL Inc.

Once such a conversion has been done, I see no reason why you
shouldn't control it over a LAN with WinVNC.

The same applies to any equipment driven by a PC - connect to a LAN
and use WinVNC. In fact, I'm told it will work with any platform
connected to the LAN - Unix, Mac, etc.

Regards,
--
Larry Stoter
JEOL (UK) Ltd
tel: +44-(0)1707-377117, fax: +44-(0)1707-373254, e-mail: larrys-at-jeoleuro.com



From daemon Wed Dec 13 15:23:14 2000



From: Pauline C. Yu :      splene-at-pw.usda.gov
Date: Wed, 13 Dec 2000 13:19:12 -0800 (PST)
Subject: Technovit 8100

Contents Retrieved from Microscopy Listserver Archives
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Dear Listers

I have a collaborator who wants to do IHC on colon tissue. She just bought
some Technovit 8100 but doesn't know how to use it. I use Technovit
7100 for regular histology on plant tissue but I have no protocol
experience with 8100, IHC or animal tissue. Does anyone have any
suggestions or recommended protocols for doing IHC with Technovit 8100?
This is for light microscopy.

Thanks
Pauline Yu
splene-at-pw.usda.gov
USDA-ARS-WRRC
Microscopist Technician




From daemon Wed Dec 13 15:47:02 2000



From: E. J. McKenzie :      elizm-at-pdx.edu
Date: Wed, 13 Dec 2000 13:42:25 -0800
Subject: need TEM film holders

Contents Retrieved from Microscopy Listserver Archives
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Hi everyone,

w'ere short about 3 plate holders for our Jeol 100cx TEM camera film (3&1/2
by 4"). At $25 apiece new, we were wondering if anyone had some spare?
Or if anyone knows which websites would have these sorts of things
second-hand?

cheers
Liz McKenzie

*******************************************************
Geomicrobiology and Electron Microscopy Laboratory
Room S9 Cramer Hall
1721 SW Broadway
Portland State University
Portland
OR97201

ph:503 725 3362
fax:503 725 3025

*******************************************************



From daemon Wed Dec 13 18:57:10 2000



From: Blanchette-Mackie, E. Joan Dr. (NIDDK) :      joanbm-at-bdg8.niddk.nih.gov
Date: Wed, 13 Dec 2000 18:46:59 -0600
Subject: Post Doctoral position at NIH

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Post Doctoral Position open in a multiple disciplinary laboratory at NIH
We use molecular biology, biochemistry and image analysis (confocal and
electron microscopy) techniques for studies on lipid transport in cells.

I am searching for someone who has training in electron microscopy to
investigate cholesterol mobilization in cells.

The position is ASAP,

The position is for a person not more than 4 years post PHD.

Those interested should send their CV's as inserts to the E-mail.

Thank You

--------------------------------------------------
Dr. Joan Blanchette-Mackie
Chief; Section of Lipid Cell Biology
NIDDK / NIH
Building 8, Room 427
8, Center Drive
Bethesda MD 20892 - 0850

Phone: (301) 496 2050
FAX: (301) 402 0723
E-mail: joanbm-at-bdg8.niddk.nih.gov





From daemon Wed Dec 13 18:57:15 2000



From: Broderick, Daniel :      dbroderick-at-masonwells.com
Date: Wed, 13 Dec 2000 18:50:36 -0600
Subject: Atom probe Microscopy

Contents Retrieved from Microscopy Listserver Archives
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Hello, I am a venture capitalist located in Milwaukee WI and am in need of
some basic information on Atom Probe Microscopy because I am evaluating a
potential investment in a company that manufactures such a device. What is
it and how is it different than TEM and SEM. What are the applications it
is used for? Are there companies that sell them? What do they cost? Any
references you can direct me to will be appreciated. Thank you.

Daniel Broderick
Managing Director
Mason Wells Biomedical
770 N. Water St.
Milwaukee WI 53202
414 765 7830
fx 414 765 7850





From daemon Wed Dec 13 22:44:40 2000



From: Mark Blackford :      mgb-at-ansto.gov.au
Date: Thu, 14 Dec 2000 15:39:03 +1100
Subject: least squares fitting of EELS spectra

Contents Retrieved from Microscopy Listserver Archives
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Hi,

I have EELS spectra from a material contain a mixture of Ce3+ and
Ce4+ and wish to determine the relative amounts of each oxidation
state. To do this I have collected spectra from a Ce3+ standard and
a Ce4+ standard and propose to least squares fit these to the
spectrum from my mixed state sample.

I presume this type of thing has been done before and there are
probably any number of ways to implement the fitting using Excel or a
KaleidaGraph or some purpose written software. I thought I'd ask if
anyone would like to make their solution available to me or even just
offer suggestions.

The ideal solution would be if the fitting could be done within EL/P
or even DigitalMicrograph.

I look forward to any replies. Cheers,

Mark Blackford
TEM Group
Materials Division, ANSTO
PMB 1,
Menai, N.S.W.
Australia
2234

Phone 61 2 9717 3027
Fax 61 2 9543 7179

Disclaimer:
The views expressed in this E-mail message do not necessarily
represent the official views of ANSTO from which this message was
conveyed.



From daemon Thu Dec 14 04:22:33 2000



From: Jim at ProSciTech :      jim-at-proscitech.com
Date: Thu, 14 Dec 2000 20:58:32 +1000
Subject: RE: Rotary shadowing

Contents Retrieved from Microscopy Listserver Archives
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The answer to this very much depends on what you are charging
for. To find an answer, you need to identify (a) the net costs you
wish to recover: e.g. capital costs (to be recovered over what
period?, at what rate of interest?), running costs, salaries of direct
(em technician) and indirect (clerical, admin.) support staff
including overheads, any charges for space occupancy and
services etc., less any subsidies (b) make a realistic estimate of
the maximum number of hours per year that a charge can be
recovered from. The answer is a/b. Simple, isn't it? Well, no. In
practise you will probably find that b is dependent on a/b since all
categories of users are sensitive to price, but you will have to
model this differently for different categories of users.

Our charges per hour for self-help access to a Philips CM120
Biotwin are as follows: Departmental £15, Other departments £21,
other publicly-funded research organisations £35, industrial £70.
Non-attenders incur full charge for the period booked. These
numbers were not exactly arrived at using rocket science. We
charge extra for all materials consumed (film, processing) and for
direct user-support from a technician. We do not include capital
costs recovery in our charges.

I would strongly advise imposing a special concessionary charge
on users who insist on tipping their specimens and retaining
circlips into the guts of the Compustage! An electronically-operated
trap door beneath the operator's seat is also an attractive option.



Chris




} From: "Ping Li" {pli-at-is.dal.ca}
To: {Microscopy-at-sparc5.microscopy.com}


I've noted no response, so I better offer something.
The technique was more commonly used to visualize stretched DNA fibers and you
may find more references using the Kleinschmitt technique about 30 years ago.

I have used fixed shadowcasting and negative staining on collagen too and both
methods "work". The advantage of rotary shadowcasting seems to be that it makes
it easier to follow the continuity of fibers (up-down, round and about). Rotary
shadowcasting is usually executed at a shallow angle of 6-12 degrees and so it
builds up more metal at the near vertical surfaces of the fibers.

Sticky-tape the very edge of the prepared specimen grid to a flat surface (half
of a microscope slide). A bit of tape may be required on two sides to assure
that the grid lies flat. Arrange the grid(s) a the rotation center of a rotator
within the vacuum chamber and locate the rotator to be between 60 and 100mm
from the evaporation source and a little lower than the source to achieve an
angle of say 10 degrees. The angle could be checked with a 10 degree card
segment.

The source could be C-Pt pellets or 0.1mm dia. Pt wire (try 40mm) wrapped
around a 1.5mm carbon cylinder about 5mm long that has been fashioned with a
suitable C rod sharpener. The "loaded" C rod is spring tensioned against a
second C rod that retains a flat end, but one that is reduced in size to about
3mm dia. using a conventional (but clean, e.g. no pencils) pencil sharpener.

Evacuate the system. Pt/C evaporation gives very fine granularity; about the
best short of special equipment, but a good vacuum ( { 5x10-5 torr) is
essential. Prior to evaporation start the rotator and adjust the speed to 30 to
60 rev/ minute. Increase the ampere control to achieve red heat, then back off
to give the vacuum a moment to recover. Then increase ampere to a white heat
and observe using a dark shield until the fashioned carbon tip has evaporated.
Hope this helps. This technique is much easier demonstrated than written about.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Wednesday, December 13, 2000 5:57 AM, Lehman, Ann
[SMTP:Ann.Lehman-at-trincoll.edu] wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear Listers,
}
} Can anyone suggest a good reference or share a technique for preparing C-Pt
} rotary shadowed collagen molecules on mica for TEM?
}
} Thanks.
}
} ----------------
} Ann Hein Lehman
} EM Facility Manager
} Trinity College
} Hartford, CT 06106
} v. 860-297-4289
} f. 860-297-2538
} e. ann.lehman-at-trincoll.edu




From daemon Thu Dec 14 09:09:26 2000



From: Humphrey, Charles :      cdh1-at-cdc.gov
Date: Thu, 14 Dec 2000 10:04:22 -0500
Subject: unsubscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Listserver,

unsubscribe.

Charles Humphrey



From daemon Thu Dec 14 10:28:03 2000



From: Sadhukhan, Pat :      SadhukhanPat-at-BFUSA.com
Date: Thu, 14 Dec 2000 10:24:30 -0600
Subject: Position: Material Scientist/Microscopist

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If you are a material scientist and have experience in microscopy and the
use of computers, then we invite you to apply for this position at the
Bridgestone/Firestone Research, Inc. in Akron, Ohio.

Our Microscopy Analysis Laboratories provide research and service in
microscopy for Bridgestone/Firestone Research, and, in general,
Bridgestone/Firestone (BFS) associates, as well as extend service to non-BFS
users. Currently, our laboratories have one 'state-of-the art' TEM/STEM/AIA,
one SEM/EDX, as well as one AFM/STM and a number of LOM-s and ancillary
equipments.

Duties of the appointee will include sample preparation, instrument
operation, analysis, and reporting. The level of appointment will depend on
the qualifications, and the experience of the successful candidate.

Please send a letter of application plus your CV (including the names,
postal-e-mail addresses, telephone/fax numbers of 3 referees) to:
Dr. Pat Sadhukhan
Bridgestone/Firestone Research, Inc. Phone: 330-379-7518
1200 Firestone Parkway Fax: 330-379-7530
Akron, OH 44317-0001



From daemon Thu Dec 14 10:34:33 2000



From: L. D. Marks :      ldm-at-risc4.numis.nwu.edu
Date: Thu, 14 Dec 2000 10:31:47 -0600 (CST)
Subject: Damage to SrTiO3

Contents Retrieved from Microscopy Listserver Archives
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Has anyone come across radiation damage to SrTiO3, either
knockon or radiolytic?

-------------------------------------------------------
Laurence Marks
Director, Center for Transportation Nanotechnology
Department of Materials Science and Engineering
Northwestern University
Tel: (847) 491-3996 Fax: (847) 491-7820
mailto:ldm-at-risc4.numis.nwu.edu http://www.numis.nwu.edu
-------------------------------------------------------

Workshop May 17-19 2001 "New approaches to the Phase Problem"
http://xraysweb.lbl.gov/esg/phasing/index.html




From daemon Thu Dec 14 10:47:06 2000



From: Mary Mager :      mager-at-interchange.ubc.ca
Date: Thu, 14 Dec 2000 08:44:02 -0800
Subject: Re: TEM user fee

Contents Retrieved from Microscopy Listserver Archives
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Dear Ping,
When we installed our TEM, we calculated the hourly cost of the service
contract, based on a 40 hour work week, and used that for a basis for our
charge to other users at the University. It is currently 50 dollars per
hour. The charge for commercial users is based on what commercial consultant
firms charge, in our area about 200 dollars an hour. There are, in my
experience, very few commercial users of TEM.
At 04:45 PM 12/13/00 -0400, you wrote:

} We just installed a Tecnai 12. I am not sure what is a reasonable charge for
} users to use the TEM. We are at university and a self-supported EM suite.
} Could you please let me know your opinion or the user fee (hourly rate)
} charged at your work place? especially at Canadian universities.
}
} Thank you.
} Ping
}
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca




From daemon Thu Dec 14 11:25:35 2000



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Thu, 14 Dec 2000 11:20:10 -0600
Subject: LN2 and the Amazing Exploding Funnel

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Hi Listers,

As a late follow-up to a somewhat recent string on liquid nitrogen safety, I
thought I would relate something that happened just this morning. While
transferring LN2 from one dewar to another using a funnel, the funnel
literally exploded just as the container was filled to the top. The
explosion was pretty forceful, sounding like a gunshot and scattering sharp
plastic shards all over the room and leaving behind a startled, but
otherwise unhurt technician (yup, it was me).

It was a standard-issue, large size plastic lab funnel, nothing special. We
had used the same funnel for over a year with no problems whatsoever. I
don't have a clue as to why it blew up or how there was enough pressure
generated to cause the explosion, but the remainder of the funnel looks a
lot like the hat Jughead used to wear in the Archie comics. It is now on
our bulletin board with a tastefully done joke notice about my recent brush
with eternity, courtesy of the other technician, Cheryl, who shall remain
anonymous.

Anyway, WATCH IT if you use the same low-tech LN2 transfer protocol we do
(or did).


Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/






From daemon Thu Dec 14 12:32:34 2000



From: Tom McKee :      tmckee-at-scilabs.com
Date: Thu, 14 Dec 2000 13:29:59 -0500
Subject: TEM-need PC based SAED indexing program users

Contents Retrieved from Microscopy Listserver Archives
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Good Morning MSA group

I am looking for comments from individuals who are currently using a PC
based program or spreadsheet to treat data from Al or Au ring SAED
calibration measurements. I would also like to be able to index minerals
such as asbestiform silicates. I am aware of older Fortran based
diffraction programs but I need a PC based system that someone is currently
using.

Thanks Tom McKee





From daemon Thu Dec 14 14:07:49 2000



From: Kalman Rubinson :      kr4-at-is2.nyu.edu
Date: Thu, 14 Dec 2000 15:02:29 -0500 (EST)
Subject: Re: wrong link for stereology book...

Contents Retrieved from Microscopy Listserver Archives
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On Wed, 13 Dec 2000 DrJohnRuss-at-aol.com-at-sparc5.microscopy.com wrote:

} Ooops - guess I need to type more carefully. The correct URL for downloading
} the Practical Stereology textbook is
} {http://members.aol.com/ImagProcTK/update.html} . The link there goes to a
} grad student's computer (someone who had enough storage space that I could
} use for a while), and from time to time that machine may go off line (when he
} is doing some "real" work), so if you don't get through at first be patient
} and try again in a few hours or the next day.

So far, today, none of the links get one to the download
site.

Kal





From daemon Thu Dec 14 14:10:00 2000



From: Zhenquan Liu :      zhenquan.liu-at-asu.edu
Date: Thu, 14 Dec 2000 13:07:40 -0700
Subject: Re: TEM user fee

Contents Retrieved from Microscopy Listserver Archives
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------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America

The fee depends on the purpose of charging.

If you just want to pay the cost of maintenance for the users inside the
university, then I think that you just make a calculation based on the
costs on liquid N2, N2 gas, films, darkroom expenses, fees for service
contract, and the other consumption (everything related to the machine,
except the salary of the professional staff). Also the fee could be quite
different from university to university. Recently I have been two
universities, the same machine, JEOL 2000FX, one charges user USD$35 per
hour, the other charges user only USD$20. As I know, when you charge user
at high price although it is reasinable, then you will get less users to
use, finally you get less "income" to cover the cost. If it happens, you
may have to lower the price to encourage users to use. This happened to one
university I used to work.

Yes, people always charge those commercial companies at much higher price,
3 - 5 times?

Do a good calculation!

Zhenquan Liu














-------------------------------------------------------------

Dear Ping,
When we installed our TEM, we calculated the hourly cost of the service
contract, based on a 40 hour work week, and used that for a basis for our
charge to other users at the University. It is currently 50 dollars per
hour. The charge for commercial users is based on what commercial consultant
firms charge, in our area about 200 dollars an hour. There are, in my
experience, very few commercial users of TEM.
At 04:45 PM 12/13/00 -0400, you wrote:

} We just installed a Tecnai 12. I am not sure what is a reasonable charge for
} users to use the TEM. We are at university and a self-supported EM suite.
} Could you please let me know your opinion or the user fee (hourly rate)
} charged at your work place? especially at Canadian universities.
}
} Thank you.
} Ping
}
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Engineering
University of British Columbia
6350 Stores Road
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648
e-mail: mager-at-interchg.ubc.ca

------------------------------------
Zhenquan Liu (Dr.)
Arizona State University
CSSS, CHEM
Room PSA213,
Tempe, AZ 85287
Tel (480) 965 4544 (o)
(480) 775 7428 (h)
Fax (480) 965 9004 (o)
Email zhenquan.liu-at-asu.edu
------------------------------------





From daemon Thu Dec 14 14:10:03 2000



From: Tim Booth :      tbooth-at-em.agr.ca
Date: Thu, 14 Dec 2000 14:17:59 -0600
Subject: Position: Virology Lab EM technician job, Winnipeg

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Canadian Food Inspection Agency
National Centre for Foreign Animal Disease
Winnipeg

Salary: $ 45,146 - $ 54,928 per year
Tenure: indeterminate

Education: Preference may be given to candidates with a diploma or
degree in biology (or a related field) from a recognized
technical college or university.
Experience: Significant experience in a research/diagnostic laboratory,
including performance of electron and light microscopy techniques.
Experience in a variety of software applications, including digital
imaging
Experience in working with infectious agents.

Willingness to work under the rigours of high security biocontainment.
Contact with livestock outside of the workplace will be subject to
certain restrictions.

Functions:

To run a busy Reserach/Diagnostic EM lab, under supervision of a
Principal Investigator:

Prepares samples for diagnostic examination by electron microscopy;
operates electron microscope and conducts standard examination
procedures for detection, identification and characterization of foreign

animal disease agents by electron microscopy; establishes and confirms
primary identification of foreign animal disease agents in emergency
disease investigations and/or recruits the required expertise to
identify unknown agents; catalogues specimens, micrographs and images
and keeps accurate and comprehensive
records; develops new technical procedures or modifies existing
procedures for sample preparation and examination including observation
of frozen hydrated specimens by electron cryomicroscopy. Works on
immunohistochemistry techniques to label and localise antigens using
both light and electron mciroscopy.

If interested please email application including CV to Dr. Tim Booth,
tbooth-at-em.agr.ca
Candidates must clearly demonstrate in writing, that they fully meet the

screening criteria. Failure to do so will result in your application
being screened out of the selection process with no further
consideration being given to your application.

---




From daemon Thu Dec 14 14:12:58 2000



From: William F. Tivol :      wft03-at-health.state.ny.us
Date: Thu, 14 Dec 2000 15:10:34 -0500
Subject: Re: LN2 and the Amazing Exploding Funnel

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html







As a late follow-up to a somewhat recent string on liquid nitrogen safety, I
thought I would relate something that happened just this morning. While
transferring LN2 from one dewar to another using a funnel, the funnel
literally exploded just as the container was filled to the top. The
explosion was pretty forceful, sounding like a gunshot and scattering sharp
plastic shards all over the room and leaving behind a startled, but
otherwise unhurt technician (yup, it was me).

It was a standard-issue, large size plastic lab funnel, nothing special. We
had used the same funnel for over a year with no problems whatsoever. I
don't have a clue as to why it blew up or how there was enough pressure
generated to cause the explosion, but the remainder of the funnel looks a
lot like the hat Jughead used to wear in the Archie comics. It is now on
our bulletin board with a tastefully done joke notice about my recent brush
with eternity, courtesy of the other technician, Cheryl, who shall remain
anonymous.

Anyway, WATCH IT if you use the same low-tech LN2 transfer protocol we do
(or did).


Dear Randy,
It is possible that internal stress was present in the funnel, and upon
cooling (this time) it built up past the ability of the funnel to adjust. This
happens in an altogether different temperature range to quarz high-pressure Hg
lamps. Teflon does not become too stiff at 77 K, so this shouldn't happen if
you use a teflon funnel.
Yours,

Bill Tivol
Wadsworth Center
Albany NY
(518) 473-7399 WFT02-at-health.state.ny.us





From daemon Thu Dec 14 14:28:21 2000



From: White, Woody N. :      nwwhite-at-mcdermott.com
Date: Thu, 14 Dec 2000 14:21:07 -0600
Subject: RE: LN2 and the Amazing Exploding Funnel

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


FWIW, I no longer use a funnel (would be S.Steel if I did). A number of
years ago I experienced the same scenario. My HDPE funnel exploded with
great force sending shrapnel ricocheting off the walls...

A bit of spilled nitrogen is not particularly hazardous (don't pour in your
shoe :) compared to the high speed funnel shards.

Woody White
McDermott Technology

-----------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} --------------------------------------------------------------
} ---------.
}
}
} Hi Listers,
}
} As a late follow-up to a somewhat recent string on liquid
} nitrogen safety, I
} thought I would relate something that happened just this
} morning. While
} transferring LN2 from one dewar to another using a funnel, the funnel
} literally exploded just as the container was filled to the top. The
} explosion was pretty forceful, sounding like a gunshot and
} scattering sharp
} plastic shards all over the room and leaving behind a startled, but
} otherwise unhurt technician (yup, it was me).
}
} It was a standard-issue, large size plastic lab funnel,
} nothing special. We
} had used the same funnel for over a year with no problems
} whatsoever. I
} don't have a clue as to why it blew up or how there was
} enough pressure
} generated to cause the explosion, but the remainder of the
} funnel looks a
} lot like the hat Jughead used to wear in the Archie comics.
} It is now on
} our bulletin board with a tastefully done joke notice about
} my recent brush
} with eternity, courtesy of the other technician, Cheryl, who
} shall remain
} anonymous.
}
} Anyway, WATCH IT if you use the same low-tech LN2 transfer
} protocol we do
} (or did).
}
}
} Randy Tindall
} EM Specialist
} Electron Microscopy Core Facility
} W122 Veterinary Medicine
} University of Missouri
} Columbia, MO 65211
} Tel: (573) 882-8304
} Fax: (573) 884-5414
} Email: tindallr-at-missouri.edu
} Web: http://www.biotech.missouri.edu/emc/
}
}
}
}



From daemon Thu Dec 14 14:49:46 2000



From: Steve Chapman :      PROTRAIN-at-CompuServe.COM
Date: Thu, 14 Dec 2000 15:46:06 -0500
Subject: S4700 and JSM 6700F (fwd)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi

Just one point on your comments relating to charge in the Hitachi 4700.
In any SEM with an in lens detector the signal will be made up of SE.
Should you use the lower detector in the Hitachi you will find an increase
in the contribution of BSE and therefore a much lower charge rate.

Training many people in the use of the 4500 and 4700 I find they tend to
become upper detector addicts, when the lower detector often has much to
offer, particularly with samples that tend towards charging.

Hope this helps?

Steve Chapman

Senior Consultant Protrain
Tel & Fax - 44 (0)1280 814774
E-mail- protrain-at-emcourses.com
Web Site - www.emcourses.com
Protrain for SEM, TEM & EDX Training World Wide with our own full time
Professional staff



From daemon Thu Dec 14 15:08:37 2000



From: Leona Cohen-Gould :      lcgould-at-mail.med.cornell.edu
Date: Thu, 14 Dec 2000 16:07:07 -0500
Subject: Re: TEM user fee

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 4:45 PM -0400 12/13/00, Ping Li wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

*********************************

Ping:
I run the EM Core facility for our medical college. I had to assure
my dean that my fees were in accord with other, similar facilities in
the NY-metro area. I called around to many of my colleagues and got
their fee schedules. My fees are about average...higher for some
things, lower for others. Briefly, our per hour fees for the EM are
$50 if the person has been trained and works alone, $75 if they need
my assistance. Film is billed at $1 each, which just barely covers
the cost...I'll probably have to raise that price this year.

If you'd like to see my whole price list (I have a fee for every
service available in the facility), you can go to to the website:
http://www.med.cornell.edu/research/cores/index.html
Go to the bottom of the page, click on the up-down arrows and scroll
to the Electron Microscopy Core.

I am responsible for covering all of the operating costs of the lab,
with the exception of my salary (thank heaven!). I am the sole
personnel in the facility for now, and handle about 100 clients/year.
I have received approval for a part-time tech, and will then offer
histology (paraffin embedding/sectioning, cryostat sectioning) as a
service rather than just having the equipment here for people to use
on their own.

Good luck. Setting up a fee schedule is no easy matter....be
prepared for loads of complaints about the cost, and people asking if
everyone pays the same rate. Everyone here pays the same fees, from
the Sr. Assoc. Dean of Research to the newest Assist. Prof. That way
no one gets their nose out of joint!

good luck,
Lee

Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175



From daemon Thu Dec 14 15:08:42 2000



From: Gerald Harrison :      jerry-at-biochem.dental.upenn.edu
Date: Thu, 14 Dec 2000 16:08:38 -0500
Subject: Hazards related to LN2

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello fellow Microscopy subscribers,

I think the point about hazards associated with LN2 that do not directly
involve the cold liquid itself is worthy of some note.

Over the 15 years we've been maintaining a LN2 cooled beryllium crystal,
there have been 3 incidents where luckily no injuries were sustained. Two
involved breaking the 4-L dewer -- once when a student smacked the top edge
of the dewer with the LN2 hose nozzle and once when a technician tripped
and fell with a full dewer. The first merely resulted in a very loud bang
with lots of fog, etc. as described in another post (no aluminum foil,
though) and very thankfully, the technician who fell was unhurt by either
the fall or the shattered glass. Again, lots of fog and running rivulets
of LN2 down the hallway.

The third incident was just a close call and one the was totally
unanticipated. Our dewer has a large cork in the top which is replaced
after the dewer is filled. After filling, the technician reached down to
pick up the nearly full dewer and, in the process, caused the LN2 to slosh
and a bit evaporated causing the cork to 'fire' out of the mouth of the
dewer (large 'pop gun' effect) and graze her face. She was not seriously
hurt beyond a small scrape on her temple...but, had she been hit in the eye
at such close range.... Anyway, since that graze incident, we who are
responsible for filling the EDAX dewer, wear glasses or goggles and are
very careful where the dewer is 'aimed' when we pick it up and carry it.

Gerald Harrison



From daemon Thu Dec 14 15:44:22 2000



From: Darrell Miles :      milesd-at-US.ibm.com
Date: Thu, 14 Dec 2000 16:41:20 -0500
Subject: Re: LN2 and the Amazing Exploding Funnel

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi,

We have had similar occurrences here. Both old, and new funnels have
exploded. It is my guess that it is internal stress in the plastic which
causes
the event (Or, should I say the rapid release of the stress from being
cooled
to below minus 200 degrees centigrade?)

What we have done, is to use only stainless steel funnels for LN2.

Darrell




From daemon Thu Dec 14 17:20:57 2000



From: Ekstrom, Harry :      harry.ekstrom-at-honeywell.com
Date: Thu, 14 Dec 2000 16:12:27 -0700
Subject: LN2 and the Amazing Exploding Funnel

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I'd say the use of a plastic funnel with LN2 is a no no anyway. It probably
just shattered from being at that extreme temperature for a certain length
of time, which is why the "explosion" coincided with the other dewar being
topped off.

Harry Ekstrom
Materials Laboratory

(602) 231-2744
e-mail: harry.ekstrom-at-honeywell.com


-----Original Message-----
} From: Tindall, Randy D. [mailto:TindallR-at-missouri.edu]
Sent: Thursday, December 14, 2000 10:20 AM
To: 'microscopy-at-sparc5.microscopy.com'


Hi Listers,

As a late follow-up to a somewhat recent string on liquid nitrogen safety, I
thought I would relate something that happened just this morning. While
transferring LN2 from one dewar to another using a funnel, the funnel
literally exploded just as the container was filled to the top. The
explosion was pretty forceful, sounding like a gunshot and scattering sharp
plastic shards all over the room and leaving behind a startled, but
otherwise unhurt technician (yup, it was me).

It was a standard-issue, large size plastic lab funnel, nothing special. We
had used the same funnel for over a year with no problems whatsoever. I
don't have a clue as to why it blew up or how there was enough pressure
generated to cause the explosion, but the remainder of the funnel looks a
lot like the hat Jughead used to wear in the Archie comics. It is now on
our bulletin board with a tastefully done joke notice about my recent brush
with eternity, courtesy of the other technician, Cheryl, who shall remain
anonymous.

Anyway, WATCH IT if you use the same low-tech LN2 transfer protocol we do
(or did).


Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/





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I'd say the use of a plastic funnel with LN2 is a no no anyway. It probably
just shattered from being at that extreme temperature for a certain length
of time, which is why the "explosion" coincided with the other dewar being
topped off.

Harry Ekstrom
Materials Laboratory

(602) 231-2744
e-mail: harry.ekstrom-at-honeywell.com


-----Original Message-----
} From: Tindall, Randy D. [mailto:TindallR-at-missouri.edu]
Sent: Thursday, December 14, 2000 10:20 AM
To: 'microscopy-at-sparc5.microscopy.com'


Hi Listers,

As a late follow-up to a somewhat recent string on liquid nitrogen safety, I
thought I would relate something that happened just this morning. While
transferring LN2 from one dewar to another using a funnel, the funnel
literally exploded just as the container was filled to the top. The
explosion was pretty forceful, sounding like a gunshot and scattering sharp
plastic shards all over the room and leaving behind a startled, but
otherwise unhurt technician (yup, it was me).

It was a standard-issue, large size plastic lab funnel, nothing special. We
had used the same funnel for over a year with no problems whatsoever. I
don't have a clue as to why it blew up or how there was enough pressure
generated to cause the explosion, but the remainder of the funnel looks a
lot like the hat Jughead used to wear in the Archie comics. It is now on
our bulletin board with a tastefully done joke notice about my recent brush
with eternity, courtesy of the other technician, Cheryl, who shall remain
anonymous.

Anyway, WATCH IT if you use the same low-tech LN2 transfer protocol we do
(or did).


Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/






From daemon Thu Dec 14 19:11:49 2000



From: Gordon Nord :      gnord-at-mindspring.com
Date: Thu, 14 Dec 2000 19:53:17 -0500
Subject: Re: TEM-need PC based SAED indexing program users

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Tom,

I am currently using Crystal Studio (check google to find the source) to
generate diffraction patterns for the amphiboles and others. Although it has
some strange characteristics the patterns are reasonable. Be advised that the
patterns are based on x-ray diffraction intensities - the actual SADs have
different intensity distributions as you might expect.

Email me if you need help indexing the asbestos silicates.

Tom McKee wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} Good Morning MSA group
}
} I am looking for comments from individuals who are currently using a PC
} based program or spreadsheet to treat data from Al or Au ring SAED
} calibration measurements. I would also like to be able to index minerals
} such as asbestiform silicates. I am aware of older Fortran based
} diffraction programs but I need a PC based system that someone is currently
} using.
}
} Thanks Tom McKee

--
Gordon Nord
Small Business Network Design and Construction
Macintosh and Windows - Solutions and Conflicts

Nord Consultants
20594 Cornstalk Terrace
Ashburn VA 20147

Voice 703-723-2798 (Home Office)
Cell 703-403-2776 (Mobile Office)
Email gnord-at-mindspring.com





From daemon Thu Dec 14 21:14:01 2000



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Thu, 14 Dec 2000 19:22:59 -0800
Subject: Fwd: RE: Rotary shadowing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Very good comment Jim

I would like just to point that it is not good idea "preheat" carbon rods
in the presence of sample. All contaminations from rods will contaminate
the sample first and than you will shadow that. Shadowing will develop not
only structural details but contamination ether. What I did, I preheat
rods without samples, than open the chamber, install the samples and pump
down the system again. Even when I done carbon film preparation, I prefer
to use the same technique. It takes more time. In general, shadowing
quality extremely depends from sample quality and sample preparation
procedure. Most air-dried protein samples demonstrate very poor structural
integrity preservation. Buffer composition is also important (you will
shadow not only the "sample" but salts from buffer as well).

Sergey


} Date: Thu, 14 Dec 2000 20:58:32 +1000
} From: Jim at ProSciTech {jim-at-proscitech.com}
} Subject: RE: Rotary shadowing
} To: "'Lehman, Ann'" {Ann.Lehman-at-trincoll.edu} ,
} "Microscopy-at-sparc5.microscopy.com" {Microscopy-at-sparc5.microscopy.com}
} Reply-to: "jim-at-proscitech.com" {jim-at-proscitech.com}
} Organization: ProSciTech
} X-Mailer: Microsoft Internet E-mail/MAPI - 8.0.0.4211
}
} ------------------------------------------------------------------------
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------------------------------------------------------

Sergey Ryazantsev, Ph.D.
Electron Microscopy
Department of Biological Chemistry, School of Medicine
University of California, Los Angeles
Box 951737
Los Angeles, CA 90095-1737

(310) 825-1144 (office)
Pager: (310) 845-0248
FAX: (310) 206-5272 (departmental)
mailto:sryazant-at-ucla.edu




From daemon Thu Dec 14 22:26:27 2000



From: Anaspec :      anaspec-at-icon.co.za
Date: Fri, 15 Dec 2000 10:15:41 +0200
Subject: FW: Remote SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have been doing some rotary shadowing to produce replicas on biological
samples for examination under the TEM. For shadowing we used V-shaped
tungsten wire and pure carbon for support,

They are a few problems we encountered. Every run is a trial and error
process. This is especially so with the setting of current to vaporize the
metal and the carbon. Every run is a different value although the voltage is
constant. At each run we try to follow the prefer parameter but somehow the
replica would look different from the previous one. Although, it is better
to observe the colour of the glow on the metal and carbon but that is very
hurting to the eye. I wonder if anybody has a definite value for the current
used to vaporize 0,1 mm pt wire/tungsten and pure carbon rod in the vacuum
chamber of 10 -5 torr.


Josephine
NUS

-----Original Message-----
} From: Jim at ProSciTech [mailto:jim-at-proscitech.com]
Sent: Thursday, December 14, 2000 6:59 PM
To: 'Lehman, Ann'; Microscopy-at-sparc5.microscopy.com




From daemon Fri Dec 15 03:49:00 2000



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Fri, 15 Dec 2000 01:55:02 -0800
Subject: Fwd: FW: Rotary shadowing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Josephine
This message is only partially related to your topic, but, anyway. This is
my comment.

I would say, it's very unlikely to have good reproducibility in your
set-up. The easiest and probably cheapest solution is to use thickness
monitor. It's relatively cheap (prices vary from $1500 to $5000),
precisely measure the thickness starting from a few angstroms, easy to
work. You may control the shutter by the thickness monitor in order to
close the shutter at some point, say 2 nm.

More "solid" solution is to use electron guns for shadowing. I am using
electron guns for decade and they shown outstanding ability to evaporate
many substances: W, Pt/C, C in my case. Electron gun evaporated carbon
films have outstanding stability (in compare with thermal evaporated) under
the beam. I would mention that I forgot the time when I have a problem with
drift. Last time it was, probably 15 years ago when I was looking for good
technique for carbon film preparation. Additional reason to switch to the
Electron Gun - sample thermal damage. Electron guns produces less
infra-reds than regular set-up.

Another issue, I would like to comment is a level of vacuum in the
evaporators. The level 5x10-5 torr in my point of view is not acceptable
for ANY applications related to biology. The abequate range started from
2x10-6 torr I believe. I would point that practically all commercially
available evaporators are able to pump system down to the good 10-6 even
without LN2 trap. 10-5 is a result of bad evaporator's maintenance. Replace
diffusion oil on SANTAVAC 5 (and forget the problems with DP), check the
O--rings for cracks, replace old O-rings (do you know how old your
system?), keep system clean, run system at least for 24 h a month. I just
could not believe that films produced at 10-5 torr would not contaminated
by hydrocarbons from oil, from that a problem, many colleagues described:
bad hydrophilic properties of the films.

Sergey

} Date: Fri, 15 Dec 2000 12:20:19 +0800
} From: "Howe L. C. Josephine" {michowej-at-nus.edu.sg}
} Subject: FW: Rotary shadowing
} To: "'microscopy-at-sparc5.microscopy.com'" {microscopy-at-sparc5.microscopy.com}
} Cc: "'jim-at-proscitech.com'" {jim-at-proscitech.com}
} X-Mailer: Internet Mail Service (5.5.2650.21)
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From daemon Fri Dec 15 05:58:13 2000



From: Jim at ProSciTech :      jim-at-proscitech.com
Date: Fri, 15 Dec 2000 21:52:22 +1000
Subject: RE: Rotary shadowing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Pt coating is not as fine as simultaneous Pt - C. Support film (carbon backing)
is only needed if you need to dissolve a thick biological specimen; for thin
fibres (say unstained biological material under 50nm) a replica is rather
cumbersome.
I expect that you also have problems with Pt forming an amalgam with W and so
varying amounts are evaporated. That is another advantage of using Pt - C.
Another problem is that for C evaporation you do best at around 30 Volts and
for metal, you achieve much better control with about 10 volts. The volt
settings on some evaporators can be changed but the variable that you are using
is changing amperes not volts. I expect that the control of one of your
electrodes is very touchy.
So take a pick, you have several problems and you may be able to eliminate one
or two.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Friday, December 15, 2000 2:20 PM, Howe L. C. Josephine
[SMTP:michowej-at-nus.edu.sg] wrote:
} We have been doing some rotary shadowing to produce replicas on biological
} samples for examination under the TEM. For shadowing we used V-shaped
} tungsten wire and pure carbon for support,
}
} They are a few problems we encountered. Every run is a trial and error
} process. This is especially so with the setting of current to vaporize the
} metal and the carbon. Every run is a different value although the voltage is
} constant. At each run we try to follow the prefer parameter but somehow the
} replica would look different from the previous one. Although, it is better
} to observe the colour of the glow on the metal and carbon but that is very
} hurting to the eye. I wonder if anybody has a definite value for the current
} used to vaporize 0,1 mm pt wire/tungsten and pure carbon rod in the vacuum
} chamber of 10 -5 torr.
}
}
} Josephine
} NUS
}
} -----Original Message-----
} From: Jim at ProSciTech [mailto:jim-at-proscitech.com]
} Sent: Thursday, December 14, 2000 6:59 PM
} To: 'Lehman, Ann'; Microscopy-at-sparc5.microscopy.com
} Subject: RE: Rotary shadowing
}
} ------------------------------------------------------------------------
} The Microscopy ListServer-Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe-Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} I've noted no response, so I better offer something. The technique was more
} commonly used to visualize stretched DNA fibers and you may find more
} references using the Kleinschmitt technique about 30 years ago.
} I have used fixed shadowcasting and negative staining on collagen too and
} both methods "work". The advantage of rotary shadowcasting seems to be that
} it makes it easier to follow the continuity of fibers (up-down, round and
} about). Rotary shadowcasting is usually executed at a shallow angle of 6-12
} degrees and so it builds up more metal at the near vertical surfaces of the
} fibers.
} Sticky-tape the very edge of the prepared specimen grid to a flat surface
} (half of a microscope slide). A bit of tape may be required on two sides to
} assure that the grid lies flat. Arrange the grid(s) a the rotation center of
} a rotator within the vacuum chamber and locate the rotator to be between 60
} and 100mm from the evaporation source and a little lower than the source to
} achieve an angle of say 10 degrees. The angle could be checked with a 10
} degree card segment.
} The source could be C-Pt pellets or 0.1mm dia. Pt wire (try 40mm) wrapped
} around a 1.5mm carbon cylinder about 5mm long that has been fashioned with a
} suitable C rod sharpener. The "loaded" C rod is spring tensioned against a
} second C rod that retains a flat end, but one that is reduced in size to
} about 3mm dia. using a conventional (but clean, e.g. no pencils) pencil
} sharpener.
} Evacuate the system. Pt/C evaporation gives very fine granularity; about the
} best short of special equipment, but a good vacuum ( { 5x10-5 torr) is
} essential. Prior to evaporation start the rotator and adjust the speed to 30
} to 60 rev/ minute. Increase the ampere control to achieve red heat, then
} back off to give the vacuum a moment to recover. Then increase ampere to a
} white heat and observe using a dark shield until the fashioned carbon tip
} has evaporated. Hope this helps. This technique is much easier demonstrated
} than written about.
} Cheers
} Jim Darley
} ProSciTech Microscopy PLUS
} PO Box 111, Thuringowa QLD 4817 Australia
} Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
} Great microscopy catalogue, 500 Links, MSDS, User Notes
} ABN: 99 724 136 560 www.proscitech.com
}
} On Wednesday, December 13, 2000 5:57 AM, Lehman, Ann
} [SMTP:Ann.Lehman-at-trincoll.edu] wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer-Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe-Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Dear Listers,
} }
} } Can anyone suggest a good reference or share a technique for preparing
} C-Pt
} } rotary shadowed collagen molecules on mica for TEM?
} }
} } Thanks.
} }
} } ----------------
} } Ann Hein Lehman
} } EM Facility Manager
} } Trinity College
} } Hartford, CT 06106
} } v. 860-297-4289
} } f. 860-297-2538
} } e. ann.lehman-at-trincoll.edu




From daemon Fri Dec 15 07:05:43 2000



From: DrJohnRuss-at-aol.com
Date: Fri, 15 Dec 2000 07:59:44 EST
Subject: Problems downloading stereology book?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


OK Folks, I am open to suggestions. The computer with the file on it is still
down, and I'm getting tired of all the nagging emails. Who can help me? The
file is 7.5 MB. Who has enough space and reasonable access and is willing to
host the file? The only conditions are that when the printed version of the
book eventually appears, the electronic on-line version will need to be
removed. In the meantime, anyone who wants to download the pdf file is
welcome. If you are interested in providing host space, please contact me
(off the list, please). Also note that there has to be some way for me to get
the file to you - many mail systems won't accept such a large attachment.

John Russ
John_Russ-at-NCSU.edu or DrJohnRuss-at-AOL.com



From daemon Fri Dec 15 07:37:01 2000



From: William.SHort-at-cpe.amedd.army.mil
Date: Fri, 15 Dec 2000 07:31:41 -0600
Subject: Ask-A-Microscopist: Verein Deutscher Ingenieur 3492 Method for

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Email: William.SHort-at-cpe.amedd.army.mil
Name: William Short

Question: One of my tasks is to implement the Verein Deutscher Ingenieur
3492 Method for analyzing asbestos fibers and other indoor pollutants
using the Scanning Electron Microscope and an Energy
Dispersive X-ray Analyzer.
At this point I am nearly completed with the implementation.
We have a nice old Hitachi S-450 model SEM and we use the KEVEX
EDX to get our spectral data off the unknown samples. I am putting
together the paperwork and reports to tell our customers what it is
that we analyzed from their sample submissions. I give a Concentration
result, the instrument Sensitivity determined by the filter area and air
volumes, the raw data, fiber types ( i.e. asbestos, inorganic, calcium
sulfate, or other), and a 95% Confidence Interval using the Poisson
distribution. Also included are the fiber dimensions, voltages,
a picture of the fiber morphology, scale in nanometers, and a copy
of a spectral spot return from the EDXA.
My problem is that I don't know if I'm producing the correct
results for the Confidence Interval calculation using the Poisson function.
Aren't there special considerations for the results obtained from getting 0
fibers,
1 fiber, 2, fibers, and 3 fibers ? Can you help me with this area or will
I just have to "punt" ? The VDI 3492 Method is being used in Europe for this
analysis to comply with the Federal Governing Standards for Host nations.

Any information that you could give me would be greatly

---------------------------------------------------------------------------





From daemon Fri Dec 15 08:21:07 2000



From: Hyman, S.C. :      sch10-at-leicester.ac.uk
Date: Fri, 15 Dec 2000 14:14:37 -0000
Subject: Metal shadowing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



On the subject of metal shadowing, might I recommend the excellent treatise
by Willison and Rowe (my ex-head of department!)
"Practical Methods in Electron Microscopy - Volume 8" - One of the
excellent Glauert edited series.



From daemon Fri Dec 15 08:38:06 2000



From: Earl Weltmer :      eweltmer-at-home.com
Date: Fri, 15 Dec 2000 06:35:32 -0800
Subject: EDS detector

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All,

I have some used EDS detectors from various SEMs that I would like to give
free to anyone who has use for them & pay the shipping costs.

They were working then stored ,uncooled for years so they are probably not
working now but might be used as a trade-in for a new system or ?

Please contact me offline if interested.

Thank You,


Earl Weltmer
(714) 573-9158




From daemon Fri Dec 15 09:27:35 2000



From: Heidi Smith :      SMITHH-at-em.agr.ca
Date: Fri, 15 Dec 2000 10:21:27 -0500
Subject: TEM film distributers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Seasons greetings to all!

I am currently in the process of ordering Kodak electron microscope film 4489 (3.25 x 4 in.), however, it seems that I can no longer order the film from Kodak Canada Inc. Kodak is still producing the film but only in the U.S. I guess. This may not seem problematic but the price of the film from the Canadian distributers is at least two times as much (almost three times in one case) as the price paid the last time it was ordered.

I believe that AGFA produces a similar type of film but I do not know the product name or who to contact. If anyone has information on distributers, of either Kodak or the AGFA film, with reasonable prices I would appreciate hearing from you. Alternatively, if anyone has another favorite TEM film that they use and would like to share that information I would be happy to hear from you.

Please send me the information offline at the following address:
smithh-at-em.agr.ca

Thank you in advance!
Heidi Smith
Microscopy Technician
Agriculture and Agri-Food Canada
Kentville, NS, Canada




From daemon Fri Dec 15 10:17:37 2000



From: Maria Ericsson :      maria_ericsson-at-hms.harvard.edu
Date: Fri, 15 Dec 2000 11:11:22 -0500
Subject: Re: TEM user fee

Contents Retrieved from Microscopy Listserver Archives
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} Dear Ping,

I've been running the Core EM Facility at Harvard Medical School in Boston
for about 5 years now. When I started the lab, I had absolutely no clue
what to charge, so I checked out what other Facilities were charging. After
a little research (but still feeling pretty clueless) I started charging
30$/ beam hour for Scope time, which I think was was below average in 1995.
There weren't that many EM labs in the area so I took an average of whoever
I got hold of across the country..

My idea was to charge a little less to get people interested. Which I think
worked well (I had good financial support for a few years, until things
took off.. I don't know if your lab there is new or already
established..) Now that people are hooked, we have an average use of 50-60
hour/month on 2 scopes. I'm charging 31.50$/hour for Med. School users and
45$/hour for outside, for assisted Scope use I charge 63$/hour for Med.
School and 94$/hour for outsiders. With our current use, which has been
pretty stable for the last year, the income from scope use covers the
service contracts and supplies..

I think my rates are lower than average, but I have a huge user group. I
have two half time technicians and the Facility income pretty much covers
the operating costs now (..not including my salary..!)

I have a full fee-for serivce pricelist at
http://www.hms.harvard.edu/core/em.html

Good luck with the EM lab!

Maria


} At 4:45 PM -0400 12/13/00, Ping Li wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America

____________________________

Maria Ericsson
Harvard Medical School EM Facility
220 Longwood Avenue
Boston, MA 02115
(617) 432 1698
maria_ericsson-at-hms.harvard.edu
http://www.hms.harvard.edu/core/em.html



From daemon Fri Dec 15 10:51:31 2000



From: Lucille A. Giannuzzi :      lag-at-mail.ucf.edu
Date: Fri, 15 Dec 2000 11:44:21 -0500
Subject: call for abstracts

Contents Retrieved from Microscopy Listserver Archives
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Call For Abstracts

Florida Society for Microscopy-Florida Chapter of the
American Vacuum Society & Surface Analysis 2001


Annual Symposium

Technical Meeting March 12-14 - Short Courses March 12-15
University of Central Florida; Student Union Building; Orlando, Florida



Meeting Highlights
* Technical Sessions: Microscopy in the Physical and Biological
Sciences, Surface Science, Thin Films, Focused Ion Beam Techniques,
Biomaterial Interfaces, Environmental Science, Metrology, and Electronic
Materials
* AVS & Vendor Sponsored Short Courses
* Job Fair
* 3rd Annual FIB Users Workshop, and Vacuum Educators Workshop
* Student Poster Competition (over $5000 in prizes can be awarded)
-plus the MSA Traveling Poster Display
* Equipment Exhibit with Surface, Analysis, Vacuum, and
MicroscopySciences Vendors
* No registration fee for Symposium or Equipment Exhibit

Confirmed Invited Speakers to Date
* Donald Baer Pacific Northwest National Lab
* Alice Dohnalkova Pacific Northwest National Lab
* Klaus Edinger University of Maryland
* Robert Hull University of Virginia
* Bill Lamberti Exxon
* Eero Ristolainen Tampere University of Technology
* Bruno Schueler Physical Electronics
* Catherine Taylor Wright State University
* Edgar Voelkl Oak Ridge National Lab



Abstract Submission
The abstract deadline is January 5, 2001 for hard copy abstracts and
January 12, 2001 for e-mail abstracts.
E-mail submissions are strongly encouraged. Contributed papers will be
presented as platform presentations
or poster presentations. Authors should indicate their session preference
and preferred presentation format
i.e., poster or platform. For uniformity, all the abstracts must be in the
following format: the title of the talk
should be in all capital letters followed by the names and addresses of the
authors. The presenter's name
should be underlined. Abstracts are limited to 200 words. E-mail
abstracts should be as text in the body of
the e-mail or an attached Microsoft Word file. Send abstracts to Dr.
Lucille Giannuzzi, Program Chair,
407 275-4354, lag-at-mail.ucf.edu. Authors will be contacted by their session
chair in mid-January 2001
concerning their abstract's status.


Registration
You may register on line by clicking on the following link:

http://natasha.eng.usf.edu/gilbert/avs/anouncement/registration.html


General Meeting Information
You may find more information by following
the "AVS Info; Chapters; Florida Chapter" links at:
www.vacuum.org or the "Local Affiliate
Societies" link at www.microscopy.org or contact Fred Stevie,
407-371-7626, stevie-at-lucent.com or Jo Ann
Moore, jamoore-at-com1.med.usf.edu 1-813-974-9446

In order to receive email updates and future
information about the meeting please forward your email address
to Pete Ries pjries-at-lucent.com.

*******************************************************************
Lucille A. Giannuzzi, Ph.D.

Associate Professor, Mechanical, Materials, and Aerospace Engineering
Director, UCF/Cirent Materials Characterization Facility
University of Central Florida
12443 Research Parkway, Suite 305
Orlando, FL 32826 email lag-at-mail.ucf.edu phone (407) 275-4354,5,6
fax (407) 275-4321
--------------------------------------------------------------------
"Good judgement comes from experience.

Experience comes from making bad judgement."

Mark Twain
********************************************************************





From daemon Fri Dec 15 11:01:49 2000



From: stemmer-at-rice.edu
Date: Fri, 15 Dec 2000 10:46:29 -0600 (CST)
Subject: Postdoctoral Position in TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Postdoctoral Position in Transmission Electron Microscopy - Rice University

Rice University, Department of Mechanical Engineering and Materials Science,
located in Houston, TX, is seeking candidates for a postdoctoral position in
transmission electron microscopy of multicomponent oxide thin films.
Applicants should have extensive and demonstrated experience in several areas
of TEM and a strong background and interest in materials problem solving.
Preference will be given to candidates with experience in high-resolution
imaging and electron energy-loss spectroscopy as well as conventional
diffraction contrast imaging.
Facilities at Rice include a JEOL 2010, field-emission SEM and high-resolution
X-ray diffractometers, as well as state-of-the-art sample preparation. The
project will be carried out in close collaboration with the University of
Houston using a state-of-the-art field-emission TEM (JEOL 2010F), with annular
dark-field detector, Oxford link EDS, Gatan GIF and STEM capabilities. Ability
and interest to take part in an upgrade of existing facilities to high
resolution scanning TEM capability is expected. The position is available
immediately. Duration about 1-2 years, salary is commensurate with
qualifications. Candidates with a Ph.D. in Materials Science or Physics will
be given preferred consideration.
Interested candidates should send a curriculum vitae, publication list and the
names of at least three references with their contact addresses to:

Prof. Susanne Stemmer
Rice University
Department of Mechanical Engineering and Materials Science
MS 321
6100 Main Street
Houston, TX 77005-1892
stemmer-at-rice.edu

Applicants must have proof of legal authorization to work in the United
States.
Rice University is an Affirmative Action/Equal Opportunity Employer.




From daemon Fri Dec 15 11:54:26 2000



From: Ron Doole :      ron.doole-at-materials.oxford.ac.uk
Date: Fri, 15 Dec 2000 17:57:51 +0000 (GMT Standard Time)
Subject: Re: TEM film distributers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Heidi,

Unfortunately Agfa have recently stopped supplying their
Scienta film that we used in some of our TEMs so we are now
changing to Kodak 4489 or SO163 as the Agfa runs out.

Ron

On Fri, 15 Dec 2000 10:21:27 -0500 Heidi Smith
{SMITHH-at-em.agr.ca} wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Seasons greetings to all!
}
} I am currently in the process of ordering Kodak electron microscope film 4489 (3.25 x 4 in.), however, it seems that I can no longer order the film from Kodak Canada Inc. Kodak is still producing the film but only in the U.S. I guess. This may not seem problematic but the price of the film from the Canadian distributers is at least two times as much (almost three times in one case) as the price paid the last time it was ordered.
}
} I believe that AGFA produces a similar type of film but I do not know the product name or who to contact. If anyone has information on distributers, of either Kodak or the AGFA film, with reasonable prices I would appreciate hearing from you. Alternatively, if anyone has another favorite TEM film that they use and would like to share that information I would be happy to hear from you.
}
} Please send me the information offline at the following address:
} smithh-at-em.agr.ca
}
} Thank you in advance!
} Heidi Smith
} Microscopy Technician
} Agriculture and Agri-Food Canada
} Kentville, NS, Canada
}
}

----------------------
Mr. R.C. Doole
Department of Materials,
University of Oxford.
Parks Road, Oxford. OX1 3PH. UK.
Phone +44 (0) 1865 273701
Fax +44 (0) 1865 283333
ron.doole-at-materials.ox.ac.uk




From daemon Fri Dec 15 11:58:16 2000



From: DrJohnRuss-at-aol.com
Date: Fri, 15 Dec 2000 12:55:19 EST
Subject: Stereology book - problem fixed!!

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


There are now several copies of the book available on different servers, so
access should not be a problem. The book is available at:

{http://loner.phys.lsu.edu/Users/brent/Stereology.pdf}
(Thanks to Brent Neal at Louisiana State Univ. in Baton Rouge. This was the
original host machine, but there was a general power failure just as I was
posting the original note - another proof of Murphy's Law - and it took a
while to get things back up; it is fine now)

{http://www.abdn.ac.uk/~ort056/stereology.pdf}
(Thanks to Jenny Gregory {j.gregory-at-abdn.ac.uk} in Aberdeen, Scotland)

{http://128.101.243.155/Stereology.pdf}
(Thanks to Mike Herron {herro001-at-umn.edu} at the University of Minnesota)

{http://www.biotech.ufl.edu/~emcl/Stereology.pdf}
(Thanks to Greg Erdos {gwe-at-biotech.ufl.edu} at the University of Florida in
Gainesville)

It should be available very soon from
{http://www.practical-stereology.org}
(Thanks to Greg Strout {gstrout-at-ou.edu} at the University of Oklahoma)

A few more sites are in process, and everything will be linked from the
original main pages at
{http://members.aol.com/drjohnruss/links.htm}
{http://members.aol.com/ImagProcTK/update.htm}

Many thanks to all of the others out there who offered help or advice.

John Russ



From daemon Fri Dec 15 14:28:17 2000



From: Shea Miller :      millers-at-em.agr.ca
Date: Fri, 15 Dec 2000 15:27:30 -0500
Subject: cryprotectants

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi everyone;
just before everyone disappears for the weekend.... I will be cutting some cryosections of infected wheat heads (on monday, of course), and wanted to fix briefly, then put into a cryoprotectant to keep the cells from rupturing when I section (for light microscopy). I know I have successfully used sucrose in the past, but I can't seem to find the concentration. (4% rings a very distant bell...). Can anyone out there tell me what works for them? (or if you have other cryoprotectants that you put your plant samples into before sectioning, I'd be interested in those, too!)

thanks, as always, in advance
shea



Dr. S.Shea Miller
Agriculture & AgriFood Canada
Eastern Cereal and Oilseed Research Centre
Rm. 2068 Neatby Building
Central Experimental Farm
Ottawa, Ontario
Canada K1A 0C6
Phone: (613) 759-1760
Fax: (613) 759-1701
E-mail: millers-at-em.agr.ca




From daemon Fri Dec 15 15:28:33 2000



From: George Langford :      amenex-at-amenex.com
Date: Fri, 15 Dec 2000 16:29:34 -0500
Subject: Re: User fee discussion

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Microscopists !

All this talk about what y'all charge your users is no different
than what prompted Congress to pass the Sherman Anti-Trust Act:

to protect Interstate commerce from price-fixing.

The discussion and setting of rates by communication
between vendors isn't proper to my legally untrained eye.

Though it's OK to publish your rates, if that's what
you really charge.

Now, it also turns out that what you charge users is nowhere
near what Amenex Associates as a private, for-profit company
has to charge to stay in business:

http://207.103.140.16/webpage/amnxfees.htm

Chuck Garber might want to chime in as to why independent lab
owners get steamed up about all this.

Best regards,
George Langford
amenex-at-amenex.com



From daemon Fri Dec 15 16:15:20 2000



From: John Chandler :      chandler-at-lamar.ColoState.EDU
Date: Fri, 15 Dec 2000 15:12:47 -0700
Subject: Request: EM images of veterinary specimens

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


To anyone who can help:

I was contacted today by a person who needs to locate some EM images of
veterinary specimens. They have an immediate need for images, probably
SEM's, of organs of the digestive system.

For further information, please contact them directly, not the listserv, at
the following:

Jill Kahn
952-852-7358
kahn-at-collemcvoy.com


I'm just the messenger,

John
john.chandler-at-colostate.edu
Colorado State University





From daemon Fri Dec 15 19:26:32 2000



From: Earl Weltmer :      eweltmer-at-home.com
Date: Fri, 15 Dec 2000 17:16:14 -0800
Subject: EDS detector

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi All,

Thank you to evryone who responded to the free EDS detector.
The two that I had are now on their way to the University of North Carolina.

I still have one EDS detector that was removed working from a PHI Auger
microprobe.
I also have some line voltage stabilizers that were removed from a JEOL 840
SEM.
Keep in mind the stabilizers are HEAVY and the shipping costs are
proportional.

Please contact me offline for details.

Thank You,

Earl Weltmer
Scanservice Corp.
SEM Maintenance
(714) 573-9158






From daemon Fri Dec 15 19:49:08 2000



From: Gary Gaugler :      gary-at-gaugler.com
Date: Fri, 15 Dec 2000 17:44:14 -0800
Subject: Re: User fee discussion

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I can see your point of concern regarding this issue. However,
if I understand the context within these folks are discussing
fees, there is a different reference basis. They are talking about
user fees in an academic environment. These fees are for
services to members of their academic community--and in
fact, for members of their respective institutions. This is quite
different from for-profit enterprises' sphere of interest.

If the universities engage in external fee-based work,
your point would tend to have merit. But what these folks
are trying to do is to spread their cost center's burden
over those who are using it, within their small community
of users.

If the situation is other than this, of course, that is a
different issue.

gary g.


At 01:29 PM 12/15/00, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From daemon Fri Dec 15 19:59:15 2000



From: Douglas Keene :      DRK-at-shcc.org
Date: Fri, 15 Dec 2000 17:56:49 -0800 (Pacific Standard Time)
Subject: rotary shadowing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I'm somewhat amazed by how many varied responses there are
on rotary shadowing technique, and how few seem to agree
with what works for us. So, here is yet another response.

We shadow biological molecules from the connective tissue
matrix, usually ranging in size from 16 to 300 kd. Many
are linear, some are globular. We spray the molecules in
solution with 70% glycerol. The other 30% is 100
microgram/ml of protein, preferably in a volatile buffer
such as 1% acetic acid or 0.1M ammonium bicarbonate, pH
7.8. Other buffers can be used but salt crystals can be a
big problem. Spraying is accomplished using an air brush,
and we spray onto freshly cleaved mica discs cut from
sheets using a hole punch.

The sample is dried in vacuum, though we often accumulate
20 samples so some drying occurs outside vacuum. We are
careful to pre-pump the vacuum chamber and heat the guns
thoroughly to out-gas, then vent the jar with nitrogen,
introduce the samples, and pump again. Our system uses a
turbo-molecular pump. We can vary the angle of the rotary
stage from outside the chamber, so we tilt the specimen
away from the gun and outgas thoroughly again so that little
vacuum loss is seen during evaporation. This is really
important. Gas is introduced to the rods whenever the
system reaches atmospheric pressure. It is also
important to keep the system clean, as outgassing a dirty
gun takes much longer than outgassing a clean gun. We try
to complete evaporation without entering the 10 -5 range,
and we begin in the 10-7 range. We evaporate at a slow
rate, usually taking 3 to 5 minutes to complete a run,
which also seems to improve resolution. Final film
resolution is very proportional to vacuum, the better the
vacuum the smaller the grain size. We use a quartz monitor
for controlling the amount of Pt-C coming from the electron
beam gun, but we also use a folded piece of filter paper
placed 90 degrees relative to the source, and monitor the
color which should be dark gray (not black, not brown). We
evaporate at 6 degrees relative to sample as the sample
rotates. Following Pt-C deposition, we then tilt the
sample to 90 degrees relative to a resistance carbon source
and evaporate a backing film of carbon onto the mica. The
thickness of the film is monitored with a piece of folded
filter paper placed 90 degrees relative to the carbon
source, and the correct amount is just visible tan color
(not gray) on the filter paper. Our film thickness
monitor is not sensitive enough to monitor carbon
deposition. We find this carbon film absolutely necessary
for sample stability, perhaps because we use so little
PT-C. However, too much carbon will certainly affect final
resolution, loosing edge detail. Finally, the replicas are
exposed to the vapors of 1% acetic acid in a petri dish for
about a half-hour prior to floating in distilled water (the
acid is very useful in helping the replica to release from
the mica (so that they float off as one intact film ). We
use high-transmission 600 mesh grids to support the films.

For many years we evaporated Pt wire from carbon rods using
a resistance source. We wound 2.3 cm of wire around a
cylindrical jig which was the same diameter as the
sharpened carbon rods (about 1mm). Prior to coiling the
wire, we passed it through a alcohol burner flame till it
was orange, which made it more malleable and perhaps cleaned
it a bit. The coil was transferred to the resistance
source, spanning the intersection of two rods
(therefore the site of most resistance and primary
heating) held together with moderate spring tension. The
carbon rods with accompanying Pt coil was at 6 degrees and
about 11 cm away from the mica discs. Using a welders
plate (Fullam #12511), we observed the metal as current was
increased through the rods and just after the wire melted,
the current was turned up just a bit more and left there
until the Pt was seen to evaporate completely. It was
necessary to observe this through the welders plate to get
a good shadow and know when to turn the current down to
avoid too much carbon. We evaporated a backing film of
pure carbon from a second source oriented at 90 degrees
from a distance of 11 cm so that the rods just started to
spark, counted to 1.5 seconds, at which time the amount of
carbon was probably about right (as judged by filter paper
color and a bit of luck).

Sorry for the novel,

Doug

----------------------
Douglas R. Keene
Associate Investigator
Shriners Hospital Research Facilities
3101 S.W. Sam Jackson Park Road
Portland, Oregon 97201
phone: 503-221-3434
FAX: 503-412-6894 (9-5 PST)
e-mail: DRK-at-shcc.org





From daemon Sat Dec 16 04:23:29 2000



From: Radostin Danev :      rado-at-nips.ac.jp
Date: Sat, 16 Dec 2000 19:17:04 +0900
Subject: Re: LN2 and the Amazing Exploding Funnel

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Stainless steel funnel poses also some danger - taking it with bare hand
after the fill (no such problem with the plastic ones). The funnel I use has
a crack which makes it more safe from an internal stress point of view.

Rado




From daemon Sat Dec 16 10:42:35 2000



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Sat, 16 Dec 2000 11:38:41 -0500
Subject: User fee discussion

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Gary Gaugler wrote:
==========================================================
I can see your point of concern regarding this issue. However, if I
understand the context within these folks are discussing fees, there is a
different reference basis. They are talking about user fees in an academic
environment. These fees are for services to members of their academic
community--and in fact, for members of their respective institutions. This
is quite different from for-profit enterprises' sphere of interest.

If the universities engage in external fee-based work, your point would tend
to have merit. But what these folks are trying to do is to spread their
cost center's burden over those who are using it, within their small
community of users.
=============================================================
I am obviously not a lawyer, however, one should always keep in mind that
Congress never granted any kind of exemptions to the anti-trust laws to tax-
exempt organizations. So George Langford is absolutely correct, none of us
should be discussing (with competitors) how we set our pricing.

Those of us who follow such things know that the penalties can be quite
severe for those who are found to be guilty of violating the anti-trust laws



From daemon Sat Dec 16 12:48:25 2000



From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Sat, 16 Dec 2000 13:46:00 -0500
Subject: User fee discussion

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Gary Gaugler wrote:
==========================================================
I can see your point of concern regarding this issue. However, if I
understand the context within these folks are discussing fees, there is a
different reference basis. They are talking about user fees in an academic
environment. These fees are for services to members of their academic
community--and in fact, for members of their respective institutions. This
is quite different from for-profit enterprises' sphere of interest.

If the universities engage in external fee-based work, your point would tend
to have merit. But what these folks are trying to do is to spread their
cost center's burden over those who are using it, within their small
community of users.
=============================================================
I am obviously not a lawyer, however, one should always keep in mind that
Congress never granted any kind of exemptions to the anti-trust laws to tax-
exempt organizations. So George Langford is absolutely correct, none of us
should be discussing (with competitors) how we set our pricing.

Those of us who follow such things know that the penalties can be quite
severe for those who are found to be guilty of violating the anti-trust laws



From daemon Sat Dec 16 15:47:36 2000



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Sun, 17 Dec 2000 10:48:24 GMT+1200
Subject: 840 success

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html





Last Friday I got my first quantitative analytical results out of the
JSM-840A which I have been converting to a 1 x eds, 3 x wds JXA
version over the past year.

This has been a big fun project, I've learned heaps, and I couldn't
have done it without this list and the help of the many listers who
have given freely of advice and suggestions, and more.

So thanks, Nestor, and all you good people out there. Please drop in
if you ever find yourselves in Auckland, New Zealand, it would be
great to be able to replace the mental images which I have of people
with factually-based ones!

cheers and happy Christmas

rtch

Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand



From daemon Sat Dec 16 16:55:19 2000



From: Gary Gaugler :      gary-at-gaugler.com
Date: Sat, 16 Dec 2000 14:48:49 -0800
Subject: Re: User fee discussion

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I do not agree with your analysis of this situation nor with
your conclusion. Drawing on a somewhat fading memory
of prior law classes (yes, I too am not a lawyer), I recollect
that antitrust is covered by the Sherman Antitrust Act.

The purpose of this Act and those is various states is to
prevent trusts from creating restraints on trade or commerce
and thus, reducing competition. The Sherman Antitrust Act
was designed to maintain economic liberty, and to [try to] eliminate
restraints on trade and competition.

The Act applies to all transactions and businesses involved in
interstate commerce. If the activities are local, the act applies
to transactions affecting interstate commerce. Therefore, as
I understand universities, they are not engaged in interstate
commerce. Neither are they engaged in commerce, per se.
Thus, they do not engage in transactions affecting interstate
commerce. Their fundamental basis of action is to recover
costs of ownership of university owned equipment and facilities
as applied to users of such items at the university. I do not
sense a wholesale effort by universities to engage in external
commerce. Note also that many of the managers are not
recovering the cost of their salaries. These are sunk costs
by their respective universities.

It therefore seems to me that antitrust does not apply to
universities. They are not engaged in commerce, they do
not affect interstate commerce, they do not effect
restraints on trade and commerce, and they are not
in competition with one another. And I think they would
agree that they are not in competition with private industry.

The law works in two ways: it specifies actions which are
allowed and may specify actions which are not allowed.
Lawyers exist because so much of the law is open to
interpretation. I've just provided my interpretation of the
law. Since I am not a lawyer, there is no charge for this.

gary g.





At 08:38 AM 12/16/00, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America




From daemon Sat Dec 16 18:32:55 2000



From: pli-at-is.dal.ca
Date: Sat, 16 Dec 2000 20:34:50 -0400
Subject: Re: Thanks for the user fee info

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I sincerely thank those of you who provided me with information and support
regarding the TEM user fee. I really appreciate it.

Regarding what "Garber, Charles A." wrote:

} .... So George Langford is absolutely correct, none of us
} should be discussing (with competitors) how we set our pricing.

I don't think this is relevant to what we have done. What we have discussed is
mostly about maintenance cost recovery, not making profit. Furthermore, we are
not competitors, and we mostly provide service for research and teaching within
our own university or institution. There is no competition among us.

Best regards,
Ping




From daemon Sat Dec 16 23:32:49 2000



From: Sergey Ryazantsev :      sryazant-at-ucla.edu
Date: Sun, 17 Dec 2000 03:16:47 -0800
Subject: Fwd: Re: User fee discussion

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Gary Gaugler wrote:

"It therefore seems to me that antitrust does not apply to
universities. They are not engaged in commerce, they do
not affect interstate commerce, they do not effect
restraints on trade and commerce, and they are not
in competition with one another. And I think they would
agree that they are not in competition with private industry."

Hmmm. This logic is quite interestinng. Then even though Universities
provide a service
in competition with private enterprise they are not competing. The logic in
all of this escapes me.
Hmmm.

----- Original Message -----
} From: "Gary Gaugler" {gary-at-gaugler.com}
To: "Garber, Charles A." {cgarber-at-2spi.com}
Cc: "MSA listserver" {Microscopy-at-sparc5.microscopy.com}
Sent: Saturday, December 16, 2000 2:48 PM


I am not friendly with American law ether, but our medical school stated as
"non-profit organization". So, to me it mean, that University is not
involved in trading and thereafter is not a subject for anti-trust law.

Our hospital, for instance, charge people for the service, but they did not
make a profit, same as when we charge people for using our facilities. I
don't see any problem here as long as we did not make profit on it.

Merry Christmas! Happy New Year and New Millennium for all ListServer
readers! I wish to all of us, that our microscopes will work better in New
Millennium and we will have enough users to recover at least 70% of
maintaining cost.

Sergey.


} Date: Sat, 16 Dec 2000 14:48:49 -0800
} From: Gary Gaugler {gary-at-gaugler.com}
} Subject: Re: User fee discussion
} X-Sender: gaugler-at-pop.calweb.com (Unverified)
} To: "Garber, Charles A." {cgarber-at-2spi.com}
} Cc: MSA listserver {Microscopy-at-sparc5.microscopy.com}
} X-Mailer: QUALCOMM Windows Eudora Version 5.0
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

------------------------------------------------------

Sergey Ryazantsev, Ph.D.
Electron Microscopy
Department of Biological Chemistry, School of Medicine
University of California, Los Angeles
Box 951737
Los Angeles, CA 90095-1737

(310) 825-1144 (office)
Pager: (310) 845-0248
FAX: (310) 206-5272 (departmental)
mailto:sryazant-at-ucla.edu




From daemon Sun Dec 17 15:59:39 2000



From: Tom Tottleben :      tomtot-at-charter.net
Date: Sun, 17 Dec 2000 15:33:05 -0600
Subject: LM Needs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Needed: Nikon Labophot 1 Epi-fluorscence lamphousing for 100w
mercury. Also interested in transformer.

Willing to trade have lots of older Zeiss 160mm items.

Thanks,
Tom





From daemon Sun Dec 17 19:10:55 2000



From: Zaluzec-at-sparc5.microscopy.com
Date: Sun, 17 Dec 2000 19:05:47 -0600
Subject: Administrivia: Reposting of a Truncated Message on User Fee's

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Colleagues

Chuck Garber's reply to the User Fee discussion was
truncated, due to a subtle effect of the Email system here.
In fairness I am reposting it for him so that you can
see the rest of his comments.

For those of you that are curious, the message was
truncated because of a line in the original text which
started with a "." followed by a blank/empty line. This
caused the Email system to believe the message was completed.

Nestor

Your Friendly Neighborhood SysOp

------------------------------------------------------


} From: "Garber, Charles A." {cgarber-at-2spi.com}
X-Mailer: E-Mail Connection v3.1a
Content-Length: 4627
Status:

=============================================================
I am obviously not a lawyer, however, one should always keep in mind that
Congress never granted any kind of exemptions to the anti-trust laws to tax-
exempt organizations. So George Langford is absolutely correct, none of us
should be discussing (with competitors) how we set our pricing.

Those of us who follow such things know that the penalties can be quite
severe for those who are found to be guilty of violating the anti-trust laws.

So to my (also) legally untrained eye, for universities to be discussing how
they set their pricing is no different than if independent laboratories
(like Amenex and Structure Probe) started a parallel discussion on how we
should be establishing our pricing. Anyone in academia who has ever written
a proposal for funding in recent years knows that universities are in direct
competition with each other in ways that are no different from the way
independent laboratories compete with each other. George alluded to another
issue, that is, the case when universities start offering their services to
private companies. I am not aware of anyone having gone to jail for this,
but it does raise enormous issues of ethical values, and the impact on
students, and their own views of what is right and what is wrong, when the
university's facilities are so commercialized. All of us should have some
concern about such matters.

Chuck

Disclaimer: Structure Probe, Inc. is an independent for-profit tax-paying
laboratory offering electron microscopy services to clients, often times in
direct competition with universities who claim they "don't compete with
private companies".

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
Structure Probe, Inc. /SPI Supplies FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: www.2spi.com
############################
==================================================





From daemon Mon Dec 18 07:38:51 2000



From: George Laing :      scisales-at-ngscorp.com
Date: Mon, 18 Dec 2000 08:29:44 -0800
Subject: RE: TEM film distributers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Agfa Scientia TEM film was discontinued earlier this year. I do not know
of a manufacturer other than Kodak that is selling a "dedicated" TEM in
North America.

George Laing
National Graphic Supply

Heidi Smith wrote:


} I believe that AGFA produces a similar type of film but I do not
} know the product name or who to contact. If anyone has
} information on distributers, of either Kodak or the AGFA film,
} with reasonable prices I would appreciate hearing from you.
} Alternatively, if anyone has another favorite TEM film that they
} use and would like to share that information I would be happy to
} hear from you.
}
}




From daemon Mon Dec 18 08:08:59 2000



From: Alison Tuling :      atuling-at-postino.up.ac.za
Date: Mon, 18 Dec 2000 08:04:55 -0600
Subject: Re: LN2 and the Amazing Exploding Funnel - an alternative.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I'd like to add my 0.004$ or R0.02 to the LN2 discussion:


An alternative to both the exploding plastic funnel and freezing finger
steel funnel is to make one out of very thick brown paper (held together
with staples rather than sticky tape).

I also staple a tissue paper filter in the funnel mouth to try to remove any
ice from the LN2.


Regards
Alison Tuling

Engineer
Industrial Metal and Minerals Research Institute
Department Materials science and Metallurgical engineering
University of Pretoria
South Africa
+27 11 420 4556





From daemon Mon Dec 18 08:43:55 2000



From: Roger Moretz :      rcmoretz-at-excite.com
Date: Mon, 18 Dec 2000 06:39:33 -0800 (PST)
Subject: Re: User fee discussion

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


It is interesting that this discussion comes up every 12 to 18 months or so.
I also note a subtle difference in the way the question was posed, and see
that the responses have, perhaps without intending, responded to both
aspects of the original question. First, it would appear that asking for
rates, particularly at potentially competitive facilities, is at least a
gray area, if not a violation of the anti-trust laws. Second, asking
assistance in how to determine how to set rates seems to be less
problematic. It is within that context that anti-trust laws would appear to
be less applicable. For individuals who have not had to deal with the
issues of making a facility self-supporting, it can seem a daunting task.
Thus, getting advice from other facility managers who have done the task
aids the requester to determine what costs must be included, how to
determine other factors, etc, and not miss any important/essential costs.
It is important for all of us to be aware of the differences in the way
questions are posed, especially as regards to this type of issue. This has
been an enlightening discussion all around.

Roger Moretz, Ph.D.
Dept of Toxicology
Boehringer Ingelheim Pharmaceuticals, Inc

NB: These are personal opinions only, and in no way reflect any other person
or organization.

On Sat, 16 Dec 2000 21:25:04 -0800, Earl Weltmer wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Gary Gaugler wrote:
}
} "It therefore seems to me that antitrust does not apply to
} universities. They are not engaged in commerce, they do
} not affect interstate commerce, they do not effect
} restraints on trade and commerce, and they are not
} in competition with one another. And I think they would
} agree that they are not in competition with private industry."
}
} Hmmm. This logic is quite interestinng. Then even though Universities
} provide a service
} in competition with private enterprise they are not competing. The logic
in
} all of this escapes me.
} Hmmm.
}
} ----- Original Message -----
} } From: "Gary Gaugler" {gary-at-gaugler.com}
} To: "Garber, Charles A." {cgarber-at-2spi.com}
} Cc: "MSA listserver" {Microscopy-at-sparc5.microscopy.com}
} Sent: Saturday, December 16, 2000 2:48 PM
} Subject: Re: User fee discussion
}
}
} }
------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
} } On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} }
-----------------------------------------------------------------------.
} }
} }
} } I do not agree with your analysis of this situation nor with
} } your conclusion. Drawing on a somewhat fading memory
} } of prior law classes (yes, I too am not a lawyer), I recollect
} } that antitrust is covered by the Sherman Antitrust Act.
} }
} } The purpose of this Act and those is various states is to
} } prevent trusts from creating restraints on trade or commerce
} } and thus, reducing competition. The Sherman Antitrust Act
} } was designed to maintain economic liberty, and to [try to] eliminate
} } restraints on trade and competition.
} }
} } The Act applies to all transactions and businesses involved in
} } interstate commerce. If the activities are local, the act applies
} } to transactions affecting interstate commerce. Therefore, as
} } I understand universities, they are not engaged in interstate
} } commerce. Neither are they engaged in commerce, per se.
} } Thus, they do not engage in transactions affecting interstate
} } commerce. Their fundamental basis of action is to recover
} } costs of ownership of university owned equipment and facilities
} } as applied to users of such items at the university. I do not
} } sense a wholesale effort by universities to engage in external
} } commerce. Note also that many of the managers are not
} } recovering the cost of their salaries. These are sunk costs
} } by their respective universities.
} }
} } It therefore seems to me that antitrust does not apply to
} } universities. They are not engaged in commerce, they do
} } not affect interstate commerce, they do not effect
} } restraints on trade and commerce, and they are not
} } in competition with one another. And I think they would
} } agree that they are not in competition with private industry.
} }
} } The law works in two ways: it specifies actions which are
} } allowed and may specify actions which are not allowed.
} } Lawyers exist because so much of the law is open to
} } interpretation. I've just provided my interpretation of the
} } law. Since I am not a lawyer, there is no charge for this.
} }
} } gary g.
} }
} }
} }
} }
} }
} } At 08:38 AM 12/16/00, you wrote:
} }
} ------------------------------------------------------------------------
} } } The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
} } } To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
} } } On-Line Help
http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} }
} -----------------------------------------------------------------------.
} } }
} } }
} } } -- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --
} } }
} } } Gary Gaugler wrote:
} } } ==========================================================
} } } I can see your point of concern regarding this issue. However, if I
} } } understand the context within these folks are discussing fees, there
is a
} } } different reference basis. They are talking about user fees in an
} academic
} } } environment. These fees are for services to members of their academic
} } } community--and in fact, for members of their respective institutions.
} This
} } } is quite different from for-profit enterprises' sphere of interest.
} } }
} } } If the universities engage in external fee-based work, your point
would
} tend
} } } to have merit. But what these folks are trying to do is to spread
their
} } } cost center's burden over those who are using it, within their small
} } } community of users.
} } } =============================================================
} } } I am obviously not a lawyer, however, one should always keep in mind
that
} } } Congress never granted any kind of exemptions to the anti-trust laws
to
} tax-
} } } exempt organizations. So George Langford is absolutely correct, none
of
} us
} } } should be discussing (with competitors) how we set our pricing.
} } }
} } } Those of us who follow such things know that the penalties can be
quite
} } } severe for those who are found to be guilty of violating the
anti-trust
} laws
} }
} }
}
}





_______________________________________________________
Send a cool gift with your E-Card
http://www.bluemountain.com/giftcenter/





From daemon Mon Dec 18 10:29:38 2000



From: Geoff McAuliffe :      mcauliff-at-umdnj.edu
Date: Mon, 18 Dec 2000 11:26:42 -0500
Subject: Re: Reposting/ User Fee's

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Zaluzec-at-sparc5.microscopy.com wrote:

} Colleagues
}
} Chuck Garber's reply to the User Fee discussion was
} truncated, due to a subtle effect of the Email system here.
} In fairness I am reposting it for him so that you can
} see the rest of his comments.
} --------------------------------------------------
}
} =============================================================
} I am obviously not a lawyer, however, one should always keep in mind that
} Congress never granted any kind of exemptions to the anti-trust laws to tax-
} exempt organizations. So George Langford is absolutely correct, none of us
} should be discussing (with competitors) how we set our pricing.

What rubbish!! Of course we can discuss what standards we use to set fees.
Businesses do this all the time.

} Those of us who follow such things know that the penalties can be quite
} severe for those who are found to be guilty of violating the anti-trust laws.

Everytime someone gets on this list (and other lists) and asks about setting
prices for users of lab services, Mr Langford and/or Dr. Garber starts making
veiled threats about anti-trust violations. Isn't it interesting that no actual
statues are cited, just vague references and generalizations, and that none of
the citers are legally trained?

} So to my (also) legally untrained eye, for universities to be discussing how
} they set their pricing is no different than if independent laboratories
} (like Amenex and Structure Probe) started a parallel discussion on how we
} should be establishing our pricing. Anyone in academia who has ever written
} a proposal for funding in recent years knows that universities are in direct
} competition with each other in ways that are no different from the way
} independent laboratories compete with each other.

Not true of NIH funding of individual investigators.

} George alluded to another
} issue, that is, the case when universities start offering their services to
} private companies.

If you will read the original posting, that is NOT what was being talked
about.

} I am not aware of anyone having gone to jail for this,

I am sure that if anyone had even been formally charged, you or Mr. Langford
would be sure to let us know!

}
} but it does raise enormous issues of ethical values, and the impact on
} students, and their own views of what is right and what is wrong, when the
} university's facilities are so commercialized. All of us should have some
} concern about such matters.

Ethical behavior is a two-way street.

} Chuck
}
} Disclaimer: Structure Probe, Inc. is an independent for-profit tax-paying
} laboratory offering electron microscopy services to clients, often times in
} direct competition with universities who claim they "don't compete with
} private companies".
}
} ===================================================
} Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
} President 1-(800)-2424-SPI
} Structure Probe, Inc. /SPI Supplies FAX: 1-(610)-436-5755
} PO BOX 656 e-mail: cgarber-at-2spi.com
} West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com
}
} Look for us!
} ############################
} WWW: www.2spi.com
} ############################
} ==================================================

Geoff
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************





From daemon Mon Dec 18 13:13:14 2000



From: Tom Pella :      tom_pella-at-tedpella.com
Date: Mon, 18 Dec 2000 11:07:47 -0800
Subject: Re: Film dryers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Regarding the contact info that Scott mentioned awhile back, we happen
to sell what is
probably that film dryer. You can see it at

http://www.tedpella.com/photo_html/photo11.htm

Tom

"Walck, Scott D." wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} There is a company called California Stainless that made film dryers that
} some of the EM supply houses sold. I bought one from them a number of years
} ago. Sorry that I don't have the contact info, but I'm sure that this is
} just what you want.
}
} -Scott
}
} Scott D. Walck, Ph.D.
} PPG Industries, Inc.
} Glass Technology Center
} Guys Run Rd. (packages)
} P. O. Box 11472 (letters)
} Pittsburgh, PA 15238-0472
}
} Walck-at-PPG.com
}
} (412) 820-8651 (office)
} (412) 820-8161 (fax)
}
} -----Original Message-----
} From: Donald Lovett [mailto:lovett-at-tcnj.edu]
} Sent: Monday, November 20, 2000 9:51 AM
} To: Microscopy.lst
} Subject: Film dryers
}
}
} ---------------------------------------------------------------
} ---------
} The Microscopy ListServer -- Sponsor: The Microscopy Society
} of America
} To Subscribe/Unsubscribe -- Send Email to
} ListServer-at-MSA.Microscopy.Com
} On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserv er/FAQ.html
}
}
}
} ---------------------------------------------------------------
} --------.
}
}
}
} Years ago I worked in a lab that had a small forced (and filtered) film
} dryer (with heater). It was just large enough to hold two racks of TEM
} negatives. I cannot find one in any of the catalogues. I can
} only find
} film dryers for 35 mm roll film. Could anyone please
} recommend a source
} of a similar dryer? (Vendors, please feel free to respond directly to
} me).
}
} Thanks,
}
} Don
}
} ______________________________________________________________________
} Donald L. Lovett e-mail: lovett-at-tcnj.edu
} Assoc. Professor, Dept. of Biology voice: (609) 771-2876
} P.O. Box 7718 fax: (609) 637-5118
} The College of New Jersey
} Ewing, NJ 08628-0718
}
}
}
}



From daemon Mon Dec 18 14:27:42 2000



From: Geoff McAuliffe :      mcauliff-at-umdnj.edu
Date: Mon, 18 Dec 2000 15:21:30 -0500
Subject: Re: User fee discussion

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Roger Moretz wrote:

} It is interesting that this discussion comes up every 12 to 18 months or so.

Yes. Some listmember asks for a little guidance and get several replys. Then two
people (the same two people, if my memory serves me) start yelling "anti-trust
violation". No facts, no applicable case law, just the suggestion of
lawlessness.

} I also note a subtle difference in the way the question was posed, and see
} that the responses have, perhaps without intending, responded to both
} aspects of the original question. First, it would appear that asking for
} rates, particularly at potentially competitive facilities, is at least a
} gray area, if not a violation of the anti-trust laws.

How? Please tell us exactly how asking what some other lab charges for XYZ is
illegal.

} Second, asking
} assistance in how to determine how to set rates seems to be less
} problematic. It is within that context that anti-trust laws would appear to
} be less applicable. For individuals who have not had to deal with the
} issues of making a facility self-supporting, it can seem a daunting task.
} Thus, getting advice from other facility managers who have done the task
} aids the requester to determine what costs must be included, how to
} determine other factors, etc, and not miss any important/essential costs.
} It is important for all of us to be aware of the differences in the way
} questions are posed, especially as regards to this type of issue. This has
} been an enlightening discussion all around.
}
} Roger Moretz, Ph.D.
} Dept of Toxicology
} Boehringer Ingelheim Pharmaceuticals, Inc
}
} NB: These are personal opinions only, and in no way reflect any other person
} or organization.
}
} On Sat, 16 Dec 2000 21:25:04 -0800, Earl Weltmer wrote:
}
} } Gary Gaugler wrote:
} }
} } "It therefore seems to me that antitrust does not apply to
} } universities. They are not engaged in commerce, they do
} } not affect interstate commerce, they do not effect
} } restraints on trade and commerce, and they are not
} } in competition with one another. And I think they would
} } agree that they are not in competition with private industry."
} }
} } Hmmm. This logic is quite interestinng. Then even though Universities
} } provide a service
} } in competition with private enterprise they are not competing. The logic
} in
} } all of this escapes me.
} } Hmmm.
} }
} } ----- Original Message -----
} } } From: "Gary Gaugler" {gary-at-gaugler.com}
} } To: "Garber, Charles A." {cgarber-at-2spi.com}
} } Cc: "MSA listserver" {Microscopy-at-sparc5.microscopy.com}
} } Sent: Saturday, December 16, 2000 2:48 PM
} } Subject: Re: User fee discussion
} }
} }
} } } I do not agree with your analysis of this situation nor with
} } } your conclusion. Drawing on a somewhat fading memory
} } } of prior law classes (yes, I too am not a lawyer), I recollect
} } } that antitrust is covered by the Sherman Antitrust Act.
} } }
} } } The purpose of this Act and those is various states is to
} } } prevent trusts from creating restraints on trade or commerce
} } } and thus, reducing competition. The Sherman Antitrust Act
} } } was designed to maintain economic liberty, and to [try to] eliminate
} } } restraints on trade and competition.
} } }
} } } The Act applies to all transactions and businesses involved in
} } } interstate commerce. If the activities are local, the act applies
} } } to transactions affecting interstate commerce. Therefore, as
} } } I understand universities, they are not engaged in interstate
} } } commerce. Neither are they engaged in commerce, per se.
} } } Thus, they do not engage in transactions affecting interstate
} } } commerce. Their fundamental basis of action is to recover
} } } costs of ownership of university owned equipment and facilities
} } } as applied to users of such items at the university. I do not
} } } sense a wholesale effort by universities to engage in external
} } } commerce. Note also that many of the managers are not
} } } recovering the cost of their salaries. These are sunk costs
} } } by their respective universities.
} } }
} } } It therefore seems to me that antitrust does not apply to
} } } universities. They are not engaged in commerce, they do
} } } not affect interstate commerce, they do not effect
} } } restraints on trade and commerce, and they are not
} } } in competition with one another. And I think they would
} } } agree that they are not in competition with private industry.
} } }
} } } The law works in two ways: it specifies actions which are
} } } allowed and may specify actions which are not allowed.
} } } Lawyers exist because so much of the law is open to
} } } interpretation. I've just provided my interpretation of the
} } } law. Since I am not a lawyer, there is no charge for this.
} } }
} } } gary g.
} } }
} } } }
} } } }
} } } } -- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --
} } } }
} } } } Gary Gaugler wrote:
} } } } ==========================================================
} } } } I can see your point of concern regarding this issue. However, if I
} } } } understand the context within these folks are discussing fees, there
} is a
} } } } different reference basis. They are talking about user fees in an
} } academic
} } } } environment. These fees are for services to members of their academic
} } } } community--and in fact, for members of their respective institutions.
} } This
} } } } is quite different from for-profit enterprises' sphere of interest.
} } } }
} } } } If the universities engage in external fee-based work, your point
} would
} } tend
} } } } to have merit. But what these folks are trying to do is to spread
} their
} } } } cost center's burden over those who are using it, within their small
} } } } community of users.
} } } } =============================================================
} } } } I am obviously not a lawyer, however, one should always keep in mind
} that
} } } } Congress never granted any kind of exemptions to the anti-trust laws
} to
} } tax-exempt organizations. So George Langford is absolutely correct, none
} of us
} } } } should be discussing (with competitors) how we set our pricing.
} } } }
} } } } Those of us who follow such things know that the penalties can be
} quite
} } } } severe for those who are found to be guilty of violating the
} anti-trust
} } laws
}
} _______________________________________________________
} Send a cool gift with your E-Card
} http://www.bluemountain.com/giftcenter/

Geoff
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************





From daemon Mon Dec 18 15:15:56 2000



From: Marilyn Howton :      mhowton-at-hsc.wvu.edu
Date: Mon, 18 Dec 2000 16:12:27 -0500
Subject: Re:LN2 and the Amazing Exploding Funnel

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Ah yes, this reminds me of a similar Close Encounter with liq N2 that I had:
I removed a stainless steel rack from a Dewar, the type used for holding 5"X5" boxes as a cell freezer. Heavy, and dripping liq N2, I placed it down on one of those gray round rubber-topped step stools found in most labs. It was quite exciting when the rubber exploded with a CRACK and sent shards of frozen rubber in every direction. Luckily, I was alone and never 'fessed up to the missing rubber top on the step stool..............
Marilyn




From daemon Mon Dec 18 16:06:20 2000



From: Chris Jeffree :      cjeffree-at-srv0.bio.ed.ac.uk
Date: Mon, 18 Dec 2000 21:53:29 -0000
Subject: F F: FW: Fwd: Fwded: Forwarded: Seasons Greetings

Contents Retrieved from Microscopy Listserver Archives
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Dear All

Best wishes for an environmentally conscious, socially responsible,
low stress, non-addictive, gender neutral, winter solstice holiday,
practiced within the most joyous traditions of the religious
persuasion of your choice, but with due respect for the religious
persuasion of others who choose to practice their own religion, as
well as those who choose not to practice a religion at all.

Additionally, (topical bit here!) a fiscally successful, personally
fulfilling, and medically uncomplicated recognition of the generally
accepted calendar year 2001, but not without due respect for the
alternative calendars of choice of other cultures whose contributions
have helped make our society great, without regard to the race,
creed, color, religious, or sexual preferences of the wishes.

May the deity of our choice bless us each and every one but should
you not be a believer (as above) then please accept our collective
wish as fellow sentient (?) beings for the full development of your
inner self.

Yours,
Chris
==================================================

(Disclaimer: This greeting is subject to confirmation, clarification or
withdrawal. It implies no promise by the sendeer actually to
implement any of the expressed wishes for her/himself or others and
no responsibility for any unintended emotional stress these
greetings may or may not bring to those not caught up in the holiday
spirit.)

Hope y'all manage to get a life this Christmas
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Dr Chris Jeffree
University of Edinburgh
Biological Sciences EM Facility
Daniel Rutherford Building
King's Buildings EDINBURGH EH9 3JH
Tel: +44 (0) 131 650 5345
FAX: +44 (0) 131 650 6563

Inveresk Cottage, 26 Carberry Road,
Inveresk, Musselburgh, Midlothian EH21 8PR, UK
Tel: +44 (0) 131 665 6062 / Mobile 07710 585 401
FAX: +44 (0) 131 653 6248
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~



From daemon Mon Dec 18 16:06:25 2000



From: Zhiping LUO :      luo-at-msd.anl.gov
Date: 18 Dec 00 15:44:32 -0800
Subject: RE: Fwd: Re: MRS follow-up

Contents Retrieved from Microscopy Listserver Archives
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alan {alan-at-nist.gov} , alg13 {alg13-at-cam.ac.uk} , alj {alj-at-clemson.edu} ,
anandh {anandh-at-metalrg.iisc.ernet.in} ,
"Anne.Sadoc" {Anne.Sadoc-at-lpms.u-cergy.fr} ,
ar {ar-at-Chemietechnik.uni-dortmund.de} ,
beeli {beeli-at-solid.phys.ethz.ch} ,
berger {berger-at-elpes.polycnrs-gre.fr} ,
berger {berger-at-lepes.polycnrs-gre.fr} ,
"Bertrand.Vandemoortele" {Bertrand.Vandemoortele-at-insa-lyon.fr} ,
chumin {chumin-at-servidor.unam.mx} , cm2 {cm2-at-andrew.cmu.edu} ,
daewoong {daewoong-at-stanford.edu} , dohkim {dohkim-at-yonsei.ac.kr} ,
dong {dong-at-dlut.edu.cn} , ehm {ehm-at-howdy.wustl.edu} ,
ezersky {ezersky-at-bgumail.bgu.ac.il} ,
"f.schurack" {f.schurack-at-ifw-dresden.de} ,
falko {falko-at-itap.physik.uni-stuttgart.de} ,
fleury {fleury-at-mail.yonsei.ac.kr} , frd {frd-at-ismanu.kiev.ua} ,
fzet {fzet-at-pdc.kth.se} ,
"Gerald.Kasner" {Gerald.Kasner-at-Physik.Uni-Magdeburg.de} ,
heilmaier {heilmaier-at-ifw-dresden.de} , hippert {hippert-at-lps.u-psud.fr} ,
hirai {hirai-at-kniri.go.jp} , horn {horn-at-fhi-berlin.mpg.de} ,
irfisher {irfisher-at-stanford.edu} , ishii {ishii-at-phys.chuo-u.ac.jp} ,
j8299701 {j8299701-at-ed.noda.sut.ac.jp} ,
kamanio {kamanio-at-metalrg.iisc.ernet.in} ,
kanazawa {kanazawa-at-u-gakugei.ac.jp} , kfk {kfk-at-howdy.wustl.edu} ,
"koshikawa.naokiyo" {koshikawa.naokiyo-at-nasda.go.jp} ,
ksun {ksun-at-uic.edu} , lueck {lueck-at-mf.mpi-stuttgart.mpg.de} ,
luo {luo-at-msd.anl.gov} , "m.feuerbacher" {m.feuerbacher-at-fz-juelich.de} ,
mae {mae-at-kristall.erdw.ethz.ch} ,
"masa-yoshimura" {masa-yoshimura-at-mgc.co.jp} ,
mboudard {mboudard-at-ltpcm.inpg.fr} , mcgrath {mcgrath-at-ssci.liv.ac.uk} ,
mdaniels {mdaniels-at-umich.edu} , mizutani {mizutani-at-nuap.nagoya-u.ac.jp} ,
"nadine.baluc" {nadine.baluc-at-psi.ch} , nieh1 {nieh1-at-llnl.gov} ,
ons {ons-at-banaras.ernet.in} , "p.ebert" {p.ebert-at-fz-juelich.de} ,
pae2 {pae2-at-lehigh.edu} ,
"r.haberkern" {r.haberkern-at-physik.tu-chemnitz.de} ,
rapin {rapin-at-lcsm.u-nancy.fr} , rivervil {rivervil-at-imr.tohoku.ac.jp} ,
rmala {rmala-at-netearth.iitd.ernet.in} , sato {sato-at-tamamori.nrim.go.jp} ,
sato {sato-at-kohsai.t.u-tokyo.ac.jp}
CC: Microscopy {Microscopy-at-sparc5.microscopy.com}
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From: Zhiping LUO :      luo-at-msd.anl.gov
Date: 18 Dec 00 15:44:32 -0800
Subject: RE: Fwd: Re: MRS follow-up

Contents Retrieved from Microscopy Listserver Archives
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Reply to: RE: Fwd: Re: MRS follow-up

Dear Experts of Quasicrystals,

Recently I received two e-mails from the MRS QC meeting organizer to this
entire group of recipients I am addressing, concerning the binary QCs.
Since the binary QCs are interesting, I would like to make some more
discussions.

In the literature, many binary QCs have been observed, including the Al-,
Cd-, Ti-, and Cr-based binary systems (to see Pr. Guyot's review, Rep.
Prog. Phys., 1991, 54, p. 1373, Table 1). The Ta- and Ga-based systems found
later are not listed there. Some of these constituents may be substituted
by other elements, not only for the Cd-Cu system (Bendersky et al.,
Scripta metall., 1987, 21, 531) but also for other alloy systems. Since the
substitution seems to be relatively easier in binary alloys, probably there
are some more chances to explore new QC alloys by the substitution method
(little changes in composition maybe needed), Among those QCs observed so
far, some may still remain unclear if they can form from the liquid
directly or not.

The interesting thing to us is that why some QCs can form from the liquid
directly. However, even if a QC forms directly from the liquid, it
doesn't mean that it must be a stable phase (for example, we recently observed
that the Mg-Zn-RE IQCs are not thermodynamically stable). Therefore, if a
QC is not thermodynamically stable, it should be more accurately renamed as
an "as-cast" QC instead of a "stable" QC.

With best regards.

Z. P. Luo





From daemon Mon Dec 18 16:53:13 2000



From: E. J. McKenzie :      elizm-at-pdx.edu
Date: Mon, 18 Dec 2000 14:49:50 -0800
Subject: Polaroid Model 550 film holder

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Hi everyone,

thanks for the deluge of TEM film holders - we have plenty now thanks.

Yet another plea - last week we noticed we were suddenly getting nasty
streaks on our SEM photos from light getting to our film holder. Close
examination revealed that the plastic casing has cracked through.

In light of the fact that lots of people are going digital with their SEM
images, does anyone have an old 550 Polaroid film holder that they are
willing to sell to us? If not, we are willing to sell off our 30 packs of
polaplan film that goes in this type of holder.

cheers
Liz McKenzie

*******************************************************
Geomicrobiology and Electron Microscopy Laboratory
Room S9 Cramer Hall
1721 SW Broadway
Portland State University
Portland
OR97201

ph:503 725 3362
fax:503 725 3025

*******************************************************



From daemon Mon Dec 18 17:19:17 2000



From: Sally Stowe :      STOWE-at-rsbs.anu.edu.au
Date: Tue, 19 Dec 2000 10:14:51 +1100
Subject: Re: F F: Seasons Greetings

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I must protest the blatant and insensitive hemispherism implicit in this
posting.

Merry er....just be happy, OK?

Sally Stowe
(Canberra, Australia, where its 30 degrees and rising).





Chris Jeffree wrote:

---

Dear All

Best wishes for an environmentally conscious, socially responsible,
low stress, non-addictive, gender neutral, winter solstice holiday,
practiced within the most joyous traditions of the religious
persuasion of your choice, but with due respect for the religious
persuasion of others who choose to practice their own religion, as
well as those who choose not to practice a religion at all.
...........
etc



From daemon Mon Dec 18 19:56:38 2000



From: Lou Solebello :      microls1297-at-mindspring.com
Date: Mon, 18 Dec 2000 18:49:51 -0600
Subject: Anti-trust laws

Contents Retrieved from Microscopy Listserver Archives
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Folks, this is the year 2000. Anti trust laws dont mean, nor do diddly
squat. It is all rhetoric spun off to make it look like things are peachy
keen. In reality it is pure B_ _ _S_ _T. Same for when the gurus talk
about how great the economy is. Look into your own wallet and assess if
you are REALLY doing better than you were 10-20 year ago. The answer is
NOT. Lou Solebello




From root Mon Dec 18 20:28:14 2000
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Folks, this is the year 2000. Anti trust laws dont mean, nor do diddly
squat. It is all rhetoric spun off to make it look like things are peachy
keen. In reality it is pure B_ _ _S_ _T. Same for when the gurus talk
about how great the economy is. Look into your own wallet and assess if
you are REALLY doing better than you were 10-20 year ago. The answer is
NOT. Lou Solebello





From daemon Mon Dec 18 20:28:21 2000



From: Ardlev-at-aol.com
Date: Mon, 18 Dec 2000 20:14:41 -0600
Subject: Viewing water in oil emulsion drops using a SEM or TEM

Contents Retrieved from Microscopy Listserver Archives
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To anyone who has experience in attempting to view and determine emulsions
bead size using a SEM and determining average particle size of a past
wax/paint sealant.

Please respond to Thomas Van Doozer tvd-at-gvtc.com
Thank you.





From daemon Tue Dec 19 01:11:33 2000



From: Geoff McAuliffe :      mcauliff-at-umdnj.edu
Date: Mon, 18 Dec 2000 11:26:42 -0500
Subject: Re: Reposting/ User Fee's

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Zaluzec-at-sparc5.microscopy.com wrote:

} Colleagues
}
} Chuck Garber's reply to the User Fee discussion was
} truncated, due to a subtle effect of the Email system here.
} In fairness I am reposting it for him so that you can
} see the rest of his comments.
} --------------------------------------------------
}
} =============================================================
} I am obviously not a lawyer, however, one should always keep in mind that
} Congress never granted any kind of exemptions to the anti-trust laws to tax-
} exempt organizations. So George Langford is absolutely correct, none of us
} should be discussing (with competitors) how we set our pricing.

What rubbish!! Of course we can discuss what standards we use to set fees.
Businesses do this all the time.

} Those of us who follow such things know that the penalties can be quite
} severe for those who are found to be guilty of violating the anti-trust laws.

Everytime someone gets on this list (and other lists) and asks about setting
prices for users of lab services, Mr Langford and/or Dr. Garber starts making
veiled threats about anti-trust violations. Isn't it interesting that no actual
statues are cited, just vague references and generalizations, and that none of
the citers are legally trained?

} So to my (also) legally untrained eye, for universities to be discussing how
} they set their pricing is no different than if independent laboratories
} (like Amenex and Structure Probe) started a parallel discussion on how we
} should be establishing our pricing. Anyone in academia who has ever written
} a proposal for funding in recent years knows that universities are in direct
} competition with each other in ways that are no different from the way
} independent laboratories compete with each other.

Not true of NIH funding of individual investigators.

} George alluded to another
} issue, that is, the case when universities start offering their services to
} private companies.

If you will read the original posting, that is NOT what was being talked
about.

} I am not aware of anyone having gone to jail for this,

I am sure that if anyone had even been formally charged, you or Mr. Langford
would be sure to let us know!

}
} but it does raise enormous issues of ethical values, and the impact on
} students, and their own views of what is right and what is wrong, when the
} university's facilities are so commercialized. All of us should have some
} concern about such matters.

Ethical behavior is a two-way street.

} Chuck
}
} Disclaimer: Structure Probe, Inc. is an independent for-profit tax-paying
} laboratory offering electron microscopy services to clients, often times in
} direct competition with universities who claim they "don't compete with
} private companies".
}
} ===================================================
} Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
} President 1-(800)-2424-SPI
} Structure Probe, Inc. /SPI Supplies FAX: 1-(610)-436-5755
} PO BOX 656 e-mail: cgarber-at-2spi.com
} West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com
}
} Look for us!
} ############################
} WWW: www.2spi.com
} ############################
} ==================================================

Geoff
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************






From daemon Tue Dec 19 06:51:59 2000



From: O. O. Ilori :      sojilori-at-oauife.edu.ng
Date: Tue, 19 Dec 2000 14:06:27 +0100 (CAT)
Subject: Interpretation of Thin film Micrographs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi guys,
Anybody who can give me tips on interpretation of thin film micrographs.
Or where I can get info on the same subject on the net.
Thanks for all your help.

Mr. O. O. ILORI
DEPARTMENT OF ELECTRONIC/ELECTRICAL ENGINEERING
OBAFEMI AWOLOWO UNIVERSITY,
ILE-IFE, OSUN STATE
NIGERIA.





From daemon Tue Dec 19 08:39:04 2000



From: Kim, Hyung Tae (NINDS) :      KimTH-at-ninds.nih.gov
Date: Tue, 19 Dec 2000 09:09:06 -0500
Subject: LM-need help low-power photomicrographs for negative images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Colleagues:

We are trying to take the low-power photomicrographs for negative images to
identify the connections past the interstitial subnucleus in the Nucleus
tractus solitarius by neuronal tracer.
Any guidance on this would be very much appreciated
Thank you in advance.


Hyung-Tae Kim

Postdoctoral Fellow
Laryngeal and Speech Section, MNB, NINDS
Bldg. 10 Rm 5D38
10 Center Drive, MSC 1416
Bethesda, MD 20892-1416

email : kimth-at-ninds.nih.gov
Phone : 301-402-0157
Fax : 301-480-0803



From daemon Tue Dec 19 08:39:05 2000



From: Leona Cohen-Gould :      lcgould-at-mail.med.cornell.edu
Date: Tue, 19 Dec 2000 09:36:21 -0500
Subject: Re: cryprotectants

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


At 3:27 PM -0500 12/15/00, Shea Miller wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

good luck,
Lee

Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175



From daemon Tue Dec 19 09:23:00 2000



From: Tim Richardson :      mirlyn-at-attglobal.net
Date: Tue, 19 Dec 2000 10:20:26 -0500
Subject: F F: FW: Fwd: Fwded: Forwarded: Seasons Greetings

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Raymond,

Here is the whole greeting!

Tim

================


Dear All

Best wishes for an environmentally conscious, socially responsible,
low stress, non-addictive, gender neutral, winter solstice holiday,
practiced within the most joyous traditions of the religious
persuasion of your choice, but with due respect for the religious
persuasion of others who choose to practice their own religion, as
well as those who choose not to practice a religion at all.

Additionally, (topical bit here!) a fiscally successful, personally
fulfilling, and medically uncomplicated recognition of the generally
accepted calendar year 2001, but not without due respect for the
alternative calendars of choice of other cultures whose contributions
have helped make our society great, without regard to the race,
creed, color, religious, or sexual preferences of the wishes.

May the deity of our choice bless us each and every one but should
you not be a believer (as above) then please accept our collective
wish as fellow sentient (?) beings for the full development of your
inner self.

Yours,
Chris
==================================================

(Disclaimer: This greeting is subject to confirmation, clarification or
withdrawal. It implies no promise by the sendeer actually to
implement any of the expressed wishes for her/himself or others and
no responsibility for any unintended emotional stress these
greetings may or may not bring to those not caught up in the holiday
spirit.)

Hope y'all manage to get a life this Christmas
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Dr Chris Jeffree
University of Edinburgh
Biological Sciences EM Facility
Daniel Rutherford Building
King's Buildings EDINBURGH EH9 3JH
Tel: +44 (0) 131 650 5345
FAX: +44 (0) 131 650 6563

Inveresk Cottage, 26 Carberry Road,
Inveresk, Musselburgh, Midlothian EH21 8PR, UK
Tel: +44 (0) 131 665 6062 / Mobile 07710 585 401
FAX: +44 (0) 131 653 6248
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~


===============================================================
Tim Richardson, R&D, Bio-Microtech Inc. & Bolton Bio-Research
email: mirlyn-at-attglobal.net, web: www.bio-microtech.com
ph: 905-951-7058 fax: 905-951-7052
4-670 Hardwick Road, P.O. Box 23, Bolton, Ontario, L7E 5T1, Canada






From daemon Tue Dec 19 11:03:51 2000



From: Chris Jeffree :      cjeffree-at-srv0.bio.ed.ac.uk
Date: Tue, 19 Dec 2000 16:50:34 -0000
Subject: (Fwd) Re: F F: FW: Fwd: Fwded: Forwarded: Seasons Greetings

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Thanks for your all your replies on this topic. Some liked it, and
some didn't but there was a substantial number of spoiled ballots, so
the jury is still out. Best wishes to you all anyway!
Chris
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Dr Chris Jeffree
University of Edinburgh
Biological Sciences EM Facility
Daniel Rutherford Building
King's Buildings EDINBURGH EH9 3JH
Tel: +44 (0) 131 650 5345
FAX: +44 (0) 131 650 6563

Inveresk Cottage, 26 Carberry Road,
Inveresk, Musselburgh, Midlothian EH21 8PR, UK
Tel: +44 (0) 131 665 6062 / Mobile 07710 585 401
FAX: +44 (0) 131 653 6248
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~



From daemon Tue Dec 19 12:21:29 2000



From: michael shaffer :      mshaf-at-darkwing.uoregon.edu
Date: Tue, 19 Dec 2000 10:16:38 -0800
Subject: EPMA+SEM: position announcement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
University of Oregon
EPMA/SEM Technician
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

The Department of Geological Sciences at the University
of Oregon invites applications for an electron beam specialist
in a modern microanalytical facility. The facility houses a
four-spectrometer Cameca SX-50 microprobe and a JEOL JSM-6300
SEM. It serves faculty and students in the Department of
Geological Sciences, Chemistry, Physics and the Materials
Science Institute. Primary responsibilities include instrument
maintenance, instruction and research collaboration.
Additional responsibilities include maintenance and operation
of support and sample preparation equipment as well as optical
microscopes and a network of computers used for file archival
and data/image analysis.

We seek an individual with expertise and experience in
WDS and EDS analytical techniques, SEM imaging and instrument
maintenance. Additional desired skills include experience
with network computers (UNIX/Windows), applied computer-aided
microscopy and instruction in instrument use. An advanced
degree in petrology, mineralogy or analytical chemistry is
desirable.

This is a full-time, annually renewable position.
Salary and title will be commensurate with experience and
education. Applicants should send curriculum vitae, a
statement of experience and interests, and the names,
addresses, phone numbers and email addresses of at least
three referees to the EPMA/SEM Search Committee, Department
of Geological Sciences, 1272 University of Oregon,
Eugene OR 97403-1272. We will begin reviewing completed
applications February 1, 2001, and will continue until the
position is filled.

The University of Oregon is an equal
opportunity/affirmative action institution committed to
cultural diversity and compliance with the Americans
with Disabilities Act.

A PDF of the text is available ... we would
genuinely appreciate this announcement's distribution.
http://epmalab.uoregon.edu/epma_position.pdf

Feel free to visit the facility's wwwsite for more
information regarding the Dep't of Geological Sciences,
the University of Oregon, and the community of Eugene.
http://epmalab.uoregon.edu/ ...

to ask any specific questions ...
about the facility: mailto:epmalab-at-darkwing.uoregon.edu
about the position: Jack Rice mailto:jrice-at-darkwing.uoregon.edu

... and a special thanx to my peers ...
http://epmalab.uoregon.edu/images/zircon-xmas.jpg

cheerios, shAf :o)

{} /\ {\/} /\ {\/} /\ {\/} /\ cogito, ergo zZOooOM /\ {\/} /\ {\/} /\ {\/} /\ {}
Michael Shaffer, R.A. - mshaf-at-darkwing.uoregon.edu
Geological Science's Electron Probe Facility - University of Oregon
http://epmalab.uoregon.edu/



From daemon Tue Dec 19 13:47:22 2000



From: Leona Cohen-Gould :      lcgould-at-mail.med.cornell.edu
Date: Tue, 19 Dec 2000 14:45:18 -0500
Subject: Re: Reposting/ User Fee's

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Some key points to the commercial guys....

We at university-based facilities are not competing with commercial
labs, nor are we out to steal any business away from you. Believe
me, most of us have our hands full trying to keep up with the
needs/demands of our in-house clients. We are here to provide
service at reasonable cost to the research faculty, students, and
staff. I know that my facility is expected to cover its
non-personnel operating costs (service contracts, supplies,
maintenance contracts) and NOT turn a profit. That is against NIH
guidelines (take a look at the tapes/transcripts of the M&M sessions
devoted to facility management). Since most if not all of my clients
are federally funded, I am not allowed to charge them more than a fee
calculated to cover my expenses for the procedure involved. My
institution maintains a separate EM facility for clinical
applications, in part because of the billing differences.
I think that the original question posted was a plea for help in
establishing de novo an approximation of what is a reasonable
price....high enough to cover operating costs and keep from operating
too far "in the red", but not so high as to be unreasonable or out of
the range for people operating within grant budgets.

It is not unreasonable for people to want to be able to get work done
without having to send it out or schlepp it half-way across town. It
has become too costly for individuals to maintain this type of
equipment in their own labs, and many people need access only on a
sporadic basis. That is why central core facilities get set up.


Chill out guys.

Lee
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175



From daemon Tue Dec 19 14:44:34 2000



From: Geoff McAuliffe :      mcauliff-at-umdnj.edu
Date: Tue, 19 Dec 2000 15:43:20 -0500
Subject: Re: LM-need help low-power photomicrographs for negative images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


"Kim, Hyung Tae (NINDS)" wrote:

} Dear Colleagues:
}
} We are trying to take the low-power photomicrographs for negative images to
} identify the connections past the interstitial subnucleus in the Nucleus
} tractus solitarius by neuronal tracer.
} Any guidance on this would be very much appreciated
} Thank you in advance.
}
}
} Hyung-Tae Kim
}
} Postdoctoral Fellow
} Laryngeal and Speech Section, MNB, NINDS
} Bldg. 10 Rm 5D38
} 10 Center Drive, MSC 1416
} Bethesda, MD 20892-1416
}
} email : kimth-at-ninds.nih.gov
} Phone : 301-402-0157
} Fax : 301-480-0803

Could you be more specific about the problems you are encountering? What is
keeping you from getting useful images?


Geoff
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************





From daemon Tue Dec 19 16:16:24 2000



From: sghoshro-at-nmsu.edu
Date: Tue, 19 Dec 2000 15:12:27 -0700 (MST)
Subject: control unit for Ultracut

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear all,

I am looking for a used control unit for Reichert Ultracut Ultramicrotome.
This is a separate box sits right next to the main microtome and some key
functions of the microtome can be controlled from it. If anyone has one
for sale, I will be very interested in buying it. Please contact me
directly, not to the listserve.

Thank you,

Soumitra



*************************************************************
Soumitra Ghoshroy Ph.D.
Electron Microscopy Lab and Fluorescence Imaging Facility
Department of Biology
Box 3EML
New Mexico State University
Las Cruces, NM 88003
Tel: 505-646-3600
Fax: 505-646-5665
e-mail:sghoshro-at-nmsu.edu
http://confocal.nmsu.edu/eml





From daemon Tue Dec 19 17:39:52 2000



From: Geinfam-at-aol.com
Date: Tue, 19 Dec 2000 18:35:46 EST
Subject: Electron microscope first demonstration

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Can anyone tell me the exact date in the "30's when the electron microscope
was first demonstrated?

geinfam-at-aol.com



From daemon Tue Dec 19 18:56:01 2000



From: Michael Nesson :      nessonm-at-ucs.orst.edu
Date: Tue, 19 Dec 2000 16:51:55 -0800
Subject: Wanted:Objectives for Zeiss SV8.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Help!
I'm looking for objective lenses for a Zeiss STEMI SV8 stereomicroscope.

The Zeiss part numbers include: 47 50 73 S 50 mm.
47 50 61 S
100 mm.
47 50 62 PLS
100 mm.
47 50 63 DS
100 mm.
47 50 72
Photo S 100 mm.
47 50 74 S
150 mm.
and 47 50 71 S 200
mm.

Please respond to me directly at nessonm-at-ucs.orst.edu with prices and
condition.
DON'T just hit the REPLY button and bother everyone else in the
newsgroup.

TIA,
M. Nesson
_______________________________________________________________________
Michael Nesson, Ph.D. Department of Biochemistry & Biophysics
2011 Ag&LS, Oregon State University, Corvallis, OR 97331-7305
(541)737-5245 FAX:(541)737-0481 nessonm-at-ucs.orst.edu





From daemon Tue Dec 19 19:49:00 2000



From: hadden-at-wingate.edu (Lee Hadden)
Date: Tue, 19 Dec 2000 20:47:47 -0500
Subject: RCA EMU 3G TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


How might I find out if there is anything we can do with our old RCA
EMU3G TEM other than simply scrapping it? How could I list it where
someone who might need it for parts would see it? It was working until
the day we stopped teaching the course here in 1990, and with the
arrival of our new SEM in January the old TEM has to go to make room for
it.

Thanks for any assistance.

Sincerely,
Lee Hadden
Professor of Biology
Department of Biology
Wingate University
Wingate, NC 28174

hadden-at-wingate.edu
http://www.wingate.edu
704-233-8238




From daemon Tue Dec 19 22:12:39 2000



From: Ekstrom, Harry :      harry.ekstrom-at-honeywell.com
Date: Tue, 19 Dec 2000 20:56:52 -0700
Subject: Electron microscope first demonstration

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


My source quotes M.Knoll first demonstrated the SEM in 1935 but no specific
date.

Harry Ekstrom
Materials Laboratory

(602) 231-2744
e-mail: harry.ekstrom-at-honeywell.com


-----Original Message-----
} From: "Geinfam-at-aol.com"-at-sparc5.microscopy.com
[mailto:"Geinfam-at-aol.com"-at-sparc5.microscopy.com]
Sent: Tuesday, December 19, 2000 4:36 PM
To: Microscopy-at-sparc5.microscopy.com


Can anyone tell me the exact date in the "30's when the electron microscope
was first demonstrated?

geinfam-at-aol.com



From daemon Wed Dec 20 02:38:49 2000



From: Feng Wu :      fwu-at-gbumail.bgu.ac.il
Date: Wed, 20 Dec 2000 10:34:51 +0200
Subject: preparation TEM sample of coral

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Can anybody tell me how to prepare TEM samples for biomaterials such as
coral?
Thanks.
Feng
**************************************************
Dr. Feng Wu
Dept. of Materials Engineering
Ben-Gurion University of the Negev
Beer-Sheva 84105, Israel

************************************************




From daemon Wed Dec 20 03:32:30 2000



From: Erwin Decraen :      erwin.decraen-at-bbri.be
Date: Wed, 20 Dec 2000 10:29:26 +0100
Subject: RE: Electron microscope first demonstration

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Philips delivered their first commercial electron microcope in October 1949
(bought by 'Statens Serum Institute' in Denmark for research into the polio
virus)

Source: FEI Company - Scope 11 - December 1999 - page 3

Decraen Erwin

Belgian Building Research Institute (BBRI)
Division of Materials
Av. P. Holoffe 21
B - 1342 Limelette

Tel. : + 32 (0)2 655 77 11
Fax : + 32 (0)2 653 07 29
E-mail : erwin.decraen-at-bbri.be {mailto:erwin.decraen-at-bbri.be}


-----Original Message-----
From: Ekstrom, Harry [SMTP:harry.ekstrom-at-honeywell.com]
Sent: Wednesday, December 20, 2000 4:57 AM
To: '"Geinfam-at-aol.com"-at-sparc5.microscopy.com';
Microscopy-at-sparc5.microscopy.com
Subject: RE: Electron microscope first demonstration


------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
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-----------------------------------------------------------------------.


My source quotes M.Knoll first demonstrated the SEM in 1935 but no
specific
date.

Harry Ekstrom
Materials Laboratory

(602) 231-2744
e-mail: harry.ekstrom-at-honeywell.com


-----Original Message-----
} From: "Geinfam-at-aol.com"-at-sparc5.microscopy.com
[mailto:"Geinfam-at-aol.com"-at-sparc5.microscopy.com]
Sent: Tuesday, December 19, 2000 4:36 PM
To: Microscopy-at-sparc5.microscopy.com
Subject: Electron microscope first demonstration



------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of
America
To Subscribe/Unsubscribe -- Send Email to
ListServer-at-MSA.Microscopy.Com
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-----------------------------------------------------------------------.


Can anyone tell me the exact date in the "30's when the electron
microscope
was first demonstrated?

geinfam-at-aol.com



From daemon Wed Dec 20 04:42:53 2000



From: Hyman, S.C. :      sch10-at-leicester.ac.uk
Date: Wed, 20 Dec 2000 10:18:28 -0000
Subject: Electron microscope first demonstration

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I have a date of Tues 07 April 1931 for the first two-stage (magnification
of an electron image further magnified by a second lens) image of a platinum
grid being obtained by Ruska.

Ref. T. Mulvey, Dept. Physics, The University of Aston, Birmingham. - An
article based upon the opening address of the fifth European congress on
electron microscopy, EMCON 72 held in Manchester 5-12 September 1972.



S.C. Hyman
Chief Technician
The Electron Microscope Laboratory
Faculty of Medicine and Biological Sciences
Adrian Building
University of Leicester
University Road
Leicester
LE1 7RH

Tel. (0116) 252 3370




-----Original Message-----
} From: Ekstrom, Harry [mailto:harry.ekstrom-at-honeywell.com]
Sent: 20 December 2000 03:57
To: '"Geinfam-at-aol.com"-at-sparc5.microscopy.com';
Microscopy-at-sparc5.microscopy.com


My source quotes M.Knoll first demonstrated the SEM in 1935 but no specific
date.

Harry Ekstrom
Materials Laboratory

(602) 231-2744
e-mail: harry.ekstrom-at-honeywell.com


-----Original Message-----
} From: "Geinfam-at-aol.com"-at-sparc5.microscopy.com
[mailto:"Geinfam-at-aol.com"-at-sparc5.microscopy.com]
Sent: Tuesday, December 19, 2000 4:36 PM
To: Microscopy-at-sparc5.microscopy.com


Can anyone tell me the exact date in the "30's when the electron microscope
was first demonstrated?

geinfam-at-aol.com



From daemon Wed Dec 20 06:17:36 2000



From: Frank Thomas :      thomasf-at-AGC.BIO.NS.CA
Date: Wed, 20 Dec 2000 08:12:04 -0400
Subject: Re: Electron microscope first demonstration

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} Can anyone tell me the exact date in the "30's when the electron
microscope
} was first demonstrated?

I have a little brochure in front of me called "The First North American
Electron Microscope", prepared by U.M. Franklin, G.C. Weatherly and G.T.
Simon, in 1978. In a section about the early European design efforts, they
state that Ruska had "constructed the pioneer two-stage transmission model
in 1933", but don't give any further information on it.

Frank Thomas
Geological Survey of Canada Atlantic
Dartmouth, Nova Scotia
Canada




From daemon Wed Dec 20 06:54:33 2000



From: Matt Ervin :      mervin-at-arl.army.mil
Date: Wed, 20 Dec 2000 07:51:48 -0500
Subject: Electron microscope first demonstration

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



I believe that Ernst Ruska invented the transmission electron microscope in
1931.




"Geinfam/-at-aol.com" on 12/19/2000 06:35:46 PM





To: Microscopy-at-sparc5.microscopy.com
cc:


Can anyone tell me the exact date in the "30's when the electron microscope
was first demonstrated?

geinfam-at-aol.com







From daemon Wed Dec 20 06:56:04 2000



From: Beavers, Roy :      rbeavers-at-post.cis.smu.edu
Date: Wed, 20 Dec 2000 06:53:50 -0600
Subject: Electron microscope first demonstration

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


My guess would be Ernst Ruska with the first electron micrograph in April
1931.
Hope this helps.

Roy Beavers
Southern Methodist University
Dept. of Geological Sciences
Electron Microprobe Lab
P.O. Box 750395
Dallas, Tx 75275
voice: 214-768-2756
fax: 214-768-2701
E-mail: rbeavers-at-mail.smu.edu


-----Original Message-----
} From: "Geinfam-at-aol.com"-at-sparc5.microscopy.com
[mailto:"Geinfam-at-aol.com"-at-sparc5.microscopy.com]
Sent: Tuesday, December 19, 2000 5:36 PM
To: Microscopy-at-sparc5.microscopy.com


Can anyone tell me the exact date in the "30's when the electron microscope
was first demonstrated?

geinfam-at-aol.com



From daemon Wed Dec 20 06:58:05 2000



From: jshields-at-cb.uga.edu
Date: Wed, 20 Dec 2000 08:00:51 -0500
Subject: SouthEastern Microscopy Society meeting info

Contents Retrieved from Microscopy Listserver Archives
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Hello!
This is to inform members and other interested individuals that the
newsletter for the Southeastern Microscopy Society is now online
at http://www.biotech.ufl.edu/sems/ . This issue contains
information about our annual meeting to be held May 23-25, 2001
at Clemson University.
The issue also includes information and forms for joining the
Society.
Thanks
John P. Shields, PhD
Center for Ultrastructural Research
151 Barrow Hall
University of Georgia
Athens, GA 30602
706-542-4080
FAX 706-542-4271
jshields-at-cb.uga.edu



From daemon Wed Dec 20 08:06:23 2000



From: Jim at ProSciTech :      jim-at-proscitech.com
Date: Wed, 20 Dec 2000 23:55:30 +1000
Subject: RE: preparation TEM sample of coral

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Eragonite or polyp? Hard or soft?
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Wednesday, December 20, 2000 6:35 PM, Feng Wu [SMTP:fwu-at-gbumail.bgu.ac.il]
wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Can anybody tell me how to prepare TEM samples for biomaterials such as
} coral?
} Thanks.
} Feng
} **************************************************
} Dr. Feng Wu
} Dept. of Materials Engineering
} Ben-Gurion University of the Negev
} Beer-Sheva 84105, Israel
}
} ************************************************
}




From daemon Wed Dec 20 08:06:28 2000



From: Jim at ProSciTech :      jim-at-proscitech.com
Date: Wed, 20 Dec 2000 23:53:05 +1000
Subject: RE: Electron microscope first demonstration

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In 1935? That was a TEM, not an SEM. Knoll was Ernst Ruska's PhD supervisor and
later collaborator. They designed and built the first TEMs.
The very first model had no specimen chamber and just proved that the lens
system could work. So the first model, which I seem to remember was before 1935
was really not a microscope.
Von Ardenne described the operating principle of an SEM in the late 30th, but I
believe none was build until the early 60th at Cambridge University, UK.
Cheers
Jim Darley
ProSciTech Microscopy PLUS
PO Box 111, Thuringowa QLD 4817 Australia
Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
Great microscopy catalogue, 500 Links, MSDS, User Notes
ABN: 99 724 136 560 www.proscitech.com

On Wednesday, December 20, 2000 1:57 PM, Ekstrom, Harry
[SMTP:harry.ekstrom-at-honeywell.com] wrote:
}
}
} My source quotes M.Knoll first demonstrated the SEM in 1935 but no specific
} date.
}
} Harry Ekstrom
} Materials Laboratory
}
} (602) 231-2744
} e-mail: harry.ekstrom-at-honeywell.com
}
}
} -----Original Message-----
} } From: "Geinfam-at-aol.com"-at-sparc5.microscopy.com
} [mailto:"Geinfam-at-aol.com"-at-sparc5.microscopy.com]
} Sent: Tuesday, December 19, 2000 4:36 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Electron microscope first demonstration
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Can anyone tell me the exact date in the "30's when the electron microscope
} was first demonstrated?
}
} geinfam-at-aol.com
}




From daemon Wed Dec 20 09:27:40 2000



From: Robert Blystone :      rblyston-at-trinity.edu
Date: Wed, 20 Dec 2000 09:23:01 -0600
Subject: EM invention

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To the list:

Concerning the invention of the electron microscope. The references
below answer the question.

Check the following Web sites.

http://mail.plymouth.edu/~biology/history/ruska.html

http://www.medhelpnet.com/medhist8.html

The reference below is definitive. A must read.
http://www.nobel.se/physics/laureates/1986/ruska-autobio.html

http://www.britannica.com/bcom/eb/article/5/0%2C5716%2C66125%201%2064460%2C
00.html

Ernst had a brother and they published together.
http://www.findarticles.com/cf_0/m0833/9216_355/62115190/p1/article.jhtml


Bob Blystone

Robert V. Blystone, Ph.D.
Professor of Biology
Trinity University
San Antonio, Texas 78212
rblyston-at-trinity.edu
210-999-7243 FAX 210-999-7229




From daemon Wed Dec 20 09:48:41 2000



From: Sara Miller :      saram-at-duke.edu
Date: Wed, 20 Dec 2000 10:37:59 -0500 (EST)
Subject: RE: Electron microscope first demonstration

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I think Ernst Ruska is generally credited for building the protootype of
today's microscope as his PhD project. He and Max Knoll published a
description in 1932 (Knoll M, Ruska E. The electron microscope. Z Physik
78:318-39, 1932.).

However, others contributed to the design and knowledge of electron
behavior and lenses, including de Broglie on wave theory (Phil Mag
47:446-58, 1924) and Busch on lenses (Ann Physik 15 (Ser 5):145-66,
1932). Also Rudenberg received patents describing and EM in 1932, and
Bruche and Johanson built the first electrostatic EM that could enlarge
images of self-luminous objects in 1932.

The first commercial EM was developed by von Borries and Ruska and
produced in Germany by Siemens and Halske in 1939, and the first
commercial EM in the US was the RCA developed by Hillier, Vance, and some
others.

This is probably more than you wanted to know, but "the" electron
microscope was really a series of developments, and today's microscopes
benefitted from additional contributions and refinements of others.

Hope this helps.
Sara Miller



On Tue, 19 Dec 2000, Ekstrom, Harry wrote:

} Date: Tue, 19 Dec 2000 20:56:52 -0700
} From: Ekstrom, Harry {harry.ekstrom-at-honeywell.com}
} To: "'\"Geinfam-at-aol.com\"-at-sparc5.microscopy.com'"
{"Geinfam-at-aol.com"-at-sparc5.microscopy.com} ,
} Microscopy-at-sparc5.microscopy.com
} Subject: RE: Electron microscope first demonstration
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} My source quotes M.Knoll first demonstrated the SEM in 1935 but no specific
} date.
}
} Harry Ekstrom
} Materials Laboratory
}
} (602) 231-2744
} e-mail: harry.ekstrom-at-honeywell.com
}
}
} -----Original Message-----
} } From: "Geinfam-at-aol.com"-at-sparc5.microscopy.com
} [mailto:"Geinfam-at-aol.com"-at-sparc5.microscopy.com]
} Sent: Tuesday, December 19, 2000 4:36 PM
} To: Microscopy-at-sparc5.microscopy.com
} Subject: Electron microscope first demonstration
}
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Can anyone tell me the exact date in the "30's when the electron microscope
} was first demonstrated?
}
} geinfam-at-aol.com
}
}

Sara E. Miller, Ph. D.
P. O. Box 3712
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-3265




From daemon Wed Dec 20 10:24:51 2000



From: Erdem Yasar :      yasar-at-turkuaz.kku.edu.tr
Date: Wed, 20 Dec 2000 18:11:42 +0200 (EET)
Subject: About tem specimen

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Dear Sir/Madam,
Can anybody tell me how to prepare TEM samples for Single crystal?
Thanks for your interested




**********************************
**********************************
** Research.Asst.ERDEM YASAR **
** University of Kirikkale **
** Department of Physics **
** TEM LABORATORY **
** 71450 KIRIKKALE/TURKEY **
** erdem.yasar-at-physics.org **
** yasar-at-science.ankara.edu.tr **
** yasar-at-turkuaz.kku.edu.tr **
**********************************
**********************************









From daemon Wed Dec 20 10:35:58 2000



From: Fortner, Jeffrey A. :      fortner-at-cmt.anl.gov
Date: Wed, 20 Dec 2000 10:32:12 -0600
Subject: RE: Electron microscope first demonstration

Contents Retrieved from Microscopy Listserver Archives
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An excellent, brief history of EM development, written by Ernst Ruska
himself (as the text of his Nobel Prize address) appears in Reviews of
Modern Physics Vol. 59 No. 3 Part I p.p. 627-638 (1987). It includes some
interesting photos, micrographs, and drawings

To answer the question, in this article Ruska states that he and Max Knoll
first demonstrated magnified electron images of a specimen on 7 April 1931.
...........................................................................
......................................................
Jeffrey A. Fortner
Argonne National Laboratory
CMT/205
9700 S. Cass Avenue
Argonne, IL 60439-4837

(630) 252-5594 (voice)
(630) 252-4771 (fax)



} ----------
} From: Jim at ProSciTech
} Reply To: jim-at-proscitech.com
} Sent: Wednesday, December 20, 2000 7:53 AM
} To: 'Ekstrom, Harry'; '"Geinfam-at-aol.com"-at-sparc5.microscopy.com';
} Microscopy-at-sparc5.microscopy.com
} Subject: RE: Electron microscope first demonstration
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} In 1935? That was a TEM, not an SEM. Knoll was Ernst Ruska's PhD
} supervisor and
} later collaborator. They designed and built the first TEMs.
} The very first model had no specimen chamber and just proved that the lens
}
} system could work. So the first model, which I seem to remember was before
} 1935
} was really not a microscope.
} Von Ardenne described the operating principle of an SEM in the late 30th,
} but I
} believe none was build until the early 60th at Cambridge University, UK.
} Cheers
} Jim Darley
} ProSciTech Microscopy PLUS
} PO Box 111, Thuringowa QLD 4817 Australia
} Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
} Great microscopy catalogue, 500 Links, MSDS, User Notes
} ABN: 99 724 136 560 www.proscitech.com
}
} On Wednesday, December 20, 2000 1:57 PM, Ekstrom, Harry
} [SMTP:harry.ekstrom-at-honeywell.com] wrote:
} }
} }
} } My source quotes M.Knoll first demonstrated the SEM in 1935 but no
} specific
} } date.
} }
} } Harry Ekstrom
} } Materials Laboratory
} }
} } (602) 231-2744
} } e-mail: harry.ekstrom-at-honeywell.com
} }
} }
} } -----Original Message-----
} } } From: "Geinfam-at-aol.com"-at-sparc5.microscopy.com
} } [mailto:"Geinfam-at-aol.com"-at-sparc5.microscopy.com]
} } Sent: Tuesday, December 19, 2000 4:36 PM
} } To: Microscopy-at-sparc5.microscopy.com
} } Subject: Electron microscope first demonstration
} }
} }
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Can anyone tell me the exact date in the "30's when the electron
} microscope
} } was first demonstrated?
} }
} } geinfam-at-aol.com
} }
}
}



From daemon Wed Dec 20 10:42:33 2000



From: James Roberts :      James.Roberts-at-mail.co.ventura.ca.us
Date: Wed, 20 Dec 2000 08:36:51 -0800
Subject: Electron microscope first demonstration

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I could not find an exact date ether but Burton and Kohl "The Electron Microscope," Reinhold publishing corp., 1942 in a chapter on History of the Electron Microscope says the following (p149) " In 1932 both types of electron microscopes were described and the first images were shown. E. Bruche and H. Hohannson produced the first electron images of an oxide cathode with an aperture lens system utilizing 300-volt electron beams. M. Knoll and E. Ruska developed the first magnetic electron microscope utilizing 60,000-volt electron beams. The theory of electron lenses was largely developed by J. Picht, O. Shcherzer and W. Henneberg." In the Acknowledgments at the start of the book Dr. Kohl (one of the authors) is said to have given a demonstration of the oxide cathodes experiments: "Such an electron microscope was demonstrated before the seminar of the McLennan Laboratory in April, 1934; this probably was the first such demonstration in Canada." according to Burton. This puts the non-scanning types back a few years before the SEM date given below. Burton and Kohl don't provide any better dates than these, that I could find. Hope this might help you in your search.
Jim.

James L. Roberts
Firearm and Toolmark Examiner
Ventura Co. Sheriff's Lab
(805) 654-2308
James.Roberts-at-mail.co.ventura.ca.us

} } } "Ekstrom, Harry" {harry.ekstrom-at-honeywell.com} 12/19/00 07:56PM } } }
------------------------------------------------------------------------
The Microscopy ListServer -- Sponsor: The Microscopy Society of America


My source quotes M.Knoll first demonstrated the SEM in 1935 but no specific
date.

Harry Ekstrom
Materials Laboratory

(602) 231-2744
e-mail: harry.ekstrom-at-honeywell.com


-----Original Message-----
} From: "Geinfam-at-aol.com"-at-sparc5.microscopy.com
[mailto:"Geinfam-at-aol.com"-at-sparc5.microscopy.com]
Sent: Tuesday, December 19, 2000 4:36 PM
To: Microscopy-at-sparc5.microscopy.com


Can anyone tell me the exact date in the "30's when the electron microscope
was first demonstrated?

geinfam-at-aol.com







From daemon Wed Dec 20 11:31:27 2000



From: Wil Bigelow :      bigelow-at-engin.umich.edu
Date: Wed, 20 Dec 2000 12:31:34 -0400
Subject: RE: EQUIP Disposal

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There are several companies that deal in used and rebuilt scientific equipment:

1. Capovani ABrothers Inc. Tel: 518-346-8347, http://www.capovani.com

2. E. McGrath Inc. Tel: 508-744-3546
35 Osborne St, Salem, MA 01970-2599

3. Scientific Exchange Tel: 613-692-1871
P.O. Box 1441, Ogdenssburg, NY 13669

4. National Sales Equipment 800-545-0540
83 Eastman St, Easton, MA 02334

5. International Equipment Trading 708-913-0777
960 Woodlands Pkwy., Vernon Hills, IL 60061
You might try one of these (some addresses and phone numbers may be out of
date), or ytou can probably find other companies that deal with used
equipment on the Internet.

Good luckn and Happy Holidays

Wilbur C. Bigelow, Prof. Emeritus
Materials Sci. & Engr., University of Michigan
Ann Arbor, MI 48109-2136 e-mail: bigelow-at-umich.edu;
Fx:734-763-4788; Ph:734-662-5237





From daemon Wed Dec 20 12:03:47 2000



From: William F. Tivol :      wft03-at-health.state.ny.us
Date: Wed, 20 Dec 2000 12:59:55 -0500
Subject: Re: About tem specimen

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Can anybody tell me how to prepare TEM samples for Single crystal?
Thanks for your interested

Dear Erdem,
It depends on the crystal and other parameters. Are you doing
selected-area (SAED), convergent beam (CBED), or reflection (RHEED) diffraction?
Is your specimen radiation resistant--such as a mineral--or labile--such as an
organic? Will it stay solid in the vacuum, or will it need to be kept at LN2
temperature (as does anthracene)? What do you need to measure, intensities or
lattice constants? What voltage will you use? The preparation can be very
simple--e.g., MgO, which is made by burning Mg and collecting the smoke on a
formvar-coated grid--or complicated--e.g., growing copper
perchlorophthalocyanine epitaxially on freshly-cleaved alkali halide from a
vapor or isolating a protein and finding crystallization conditions that produce
well-ordered platy single crystals. Let me know some details, and I'll see if I
can help.
Yours,

Bill Tivol
Wadsworth Center
Albany NY
(518) 473-7399 WFT02-at-health.state.ny.us





From daemon Wed Dec 20 12:04:30 2000



From: chris smith (IACR-RES) :      chris.smith-at-bbsrc.ac.uk
Date: Wed, 20 Dec 2000 18:00:58 -0000
Subject: LN2 funnels, etc

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Hi Listers,

Many thanks for the awful warnings, confessions and suggestions. We use two
ordinary 9cm plastic funnels which have cracked and one 20cm funnel labelled
KENUTUF, supplied with the mic to top up the Xray analysis dewar, which
still seems as good as new after at least six years.

I love the Origami solution but to replace the little funnels I'll go with
the stainless type for durability and add an insulated handle.

On the bung popping dewar front, the answer is not tin hats and a shout of
'incoming' but to provide a small vent. Even pressurised dewars need a
relief valve.

ttfn, Chris



From daemon Wed Dec 20 12:24:51 2000



From: Clow, Patricia :      Patricia.Clow-at-genzyme.com
Date: Wed, 20 Dec 2000 13:04:28 -0500
Subject: preparation TEM sample of coral

Contents Retrieved from Microscopy Listserver Archives
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Try Dr. Joseph L. Scott at The College of William and Mary (Williamsburg,
VA)...
jlscot-at-facstaff.wm.edu
His research includes TEM of coralline algae.

Patty Clow

~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Patricia A. Clow, Ph.D.
Staff Scientist I / Microscopy
Genzyme Corporation
One Mountain Road
PO Box 9322
Framingham, MA 01701-9322
phone: 508-270-2053
fax: 508-872-9080
email: patricia.clow-at-genzyme.com



-----Original Message-----
} From: Feng Wu [mailto:fwu-at-gbumail.bgu.ac.il]
Sent: Wednesday, December 20, 2000 3:35 AM
To: Microscopy-at-sparc5.microscopy.com


Can anybody tell me how to prepare TEM samples for biomaterials such as
coral?
Thanks.
Feng
**************************************************
Dr. Feng Wu
Dept. of Materials Engineering
Ben-Gurion University of the Negev
Beer-Sheva 84105, Israel

************************************************




From daemon Wed Dec 20 12:32:39 2000



From: NPGSlithography-at-aol.com
Date: Wed, 20 Dec 2000 13:29:27 EST
Subject: Re: User fee discussion

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Dear All,

The current discussion of user fees appears to have strong, contrary
opinions, so it seems worthwhile to take a look at what is posted at the US
Department of Justice web site.

The following is taken "http://www.usdoj.gov/atr/foia/divisionmanual/ch2.htm".

} } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } { { { { { { {
1. SHERMAN ANTITRUST ACT, 15 U.S.C. §§ 1-7

§ 1 Sherman Act, 15 U.S.C. § 1

Trusts, etc., in restraint of trade illegal; penalty

Every contract, combination in the form of trust or otherwise, or conspiracy,
in restraint of trade or commerce among the several States, or with foreign
nations, is declared to be illegal. Every person who shall make any contract
or engage in any combination or conspiracy hereby declared to be illegal
shall be deemed guilty of a felony, and, on conviction thereof, shall be
punished by fine not exceeding $10,000,000 if a corporation, or, if any other
person, $350,000, or by imprisonment not exceeding three years, or by both
said punishments, in the discretion of the court.

§ 2 Sherman Act, 15 U.S.C. § 2

Monopolizing trade a felony; penalty

Every person who shall monopolize, or attempt to monopolize, or combine or
conspire with any other person or persons, to monopolize any part of the
trade or commerce among the several States, or with foreign nations, shall be
deemed guilty of a felony, and, on conviction thereof, shall be punished by
fine not exceeding $10,000,000 if a corporation, or, if any other person,
$350,000, or by imprisonment not exceeding three years, or by both said
punishments, in the discretion of the court.

(There are many more sections, but I believe these two summarize the main
issues of the Act.)
{ { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { {

Based on a common sense reading of the above, it seems to me that "restraint
of trade" and the "attempt to monopolize" are the key factors that that
Sherman Antitrust Act is designed to prevent, however, the issue of "intent"
is not obvious.

I would think that a discussion by EM facility managers about how they charge
for the use of their facilities and services, where the main emphasis seems
to be how to cover the expenses of running the facilities, does not appear to
be an intentional "restraint of trade" or an "attempt to monopolize" their
trade.

I would agree that any suggestion of price fixing or how to undercut the
rates of private companies would certainly violate the Sherman Antitrust Act,
but I hardly think anyone can argue that any such comments have been made in
the current discussion.

However, the question remains that if the well intentioned discussions
between managers of nonprofit and/or university supported facilities on how
to charge to cover their operating costs results in wide spread rates that
actually do undercut private companies, is that illegal? I will refrain from
presenting my opinion, since I believe that only someone with the appropriate
legal background can give an authoritative answer to this question. And
considering that every lawsuit has lawyers representing the opposing sides,
the answer may only be clear if there is already a precedent on record where
a similar case has actually gone through the courts.

Joe
_________________________________________
Joe Nabity, Ph.D.
JC Nabity Lithography Systems
E-Beam Lithography using Commercial SEMs & STEMs
PO Box 5354, Bozeman, MT 59717 USA
Voice: (406) 587-0848
FAX: (406) 586-9514
E-mail: info-at-jcnabity.com
Web: www.jcnabity.com



From daemon Wed Dec 20 12:41:54 2000



From: Jo Ann Moore :      jamoore-at-hsc.usf.edu
Date: Wed, 20 Dec 2000 13:46:16 -0500
Subject: Aus Jena condenser

Contents Retrieved from Microscopy Listserver Archives
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Fellow microscopists,

We are looking for a condenser for an old light microscope, an Aus Jena
brand. Does anyone know where we might find such an item?

Thanks, Jo Ann Moore
University of South Florida
Anatomy Department




From daemon Wed Dec 20 14:45:54 2000



From: JHumenansky-at-phi.com
Date: Wed, 20 Dec 2000 14:39:24 -0600
Subject: Re: Reposting/ User Fee's

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Personnel salaries ARE part of the operating costs of running any type of
laboratory facility. In addition, the cost of an electron microscope is
also part of the operating expense of operating a lab. To suggest that
only the non personnel costs such as service contracts, supplies etc.
should be counted as operating costs really distorts the true picture.

I came from a university lab that originally was small and able to meet all
of its operating expenses including salaries. Eventually visionaries saw
the need for a larger more grand facility that would include some
instruments with an annual service contract around 35K, other contract
costs were in the range of 9K to 20K per year. The facility was built and
the rates were kept low for academic users (about $25/hr.) and rates for
non university users were scaled depending upon association with the
facility. It didn't matter that it required about 1400 hours of beam time
on one instrument just to pay the service contract because the facility
received 8-10 million dollars per year in support from NSF. Principle
instruments were obtained in grants and other subsidies not related to
operating expenses. Eventually the NSF contract expired and now with aging
equipment technology is passing them by.

The point of this is that nearly all university facilities will eventually
have to be accountable for all of their expenses and adjust their rates to
cover the costs. But universities still will have an advantage in that
they usually don't have to purchase the principle equipment from operating
expenses. How NSF granted instrumentation can be used is described in NSF
Important Rule 91.

When university labs have to account for all of their operating expenses,
they will become competitors of private non-subsidized labs. If they offer
instruments rates less than private lab, they are cheating.

John Humenansky/Staff Scientist
Physical Electronics, Inc. (PHI)
6509 Flying Cloud Drive
Eden Prairie, MN 55344
952-828-6387




From daemon Wed Dec 20 15:13:38 2000



From: Scott Whittaker :      Whittaker.scott-at-nmnh.si.edu
Date: Wed, 20 Dec 2000 16:08:19 -0500
Subject: lab design book

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I get a rare opportunity to renovate some space to move our EM suite and have to turn in the ideal lab concept very soon. I know there is a book on this very thing. Anyone recall the title and author??




From daemon Wed Dec 20 15:19:50 2000



From: Scott Whittaker :      Whittaker.scott-at-nmnh.si.edu
Date: Wed, 20 Dec 2000 16:15:13 -0500
Subject: ESEM users

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I am now in charge of a Phillips XL30 ESEM and have a couple of questions. What are you doing to prevent contamination of the specimen chamber. I had a user wanting to view human tissue today unfixed and am reluctant to do so. Anyone have a solution. Mine was to fix and process for standard SEM but that may not be an option for some of the in-situ studies or preserved museum specimens they don't want to wash or damage. Thanks




From daemon Wed Dec 20 15:19:51 2000



From: John Hunt :      hunt-at-ccmr.cornell.edu
Date: Wed, 20 Dec 2000 16:17:44 -0500
Subject: Re: LN2 funnels, etc

Contents Retrieved from Microscopy Listserver Archives
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} Small stainless steel funnels are used to fill many LN2
} anticontamination devices on electron microscopes. I have found that a
} Java jacket (designed for holding hot coffee cups) makes a great
} reuseable jacket for picking up these funnels when they are cold.

John Hunt
CCMR Microscopy Facility
255-0108




From daemon Wed Dec 20 15:39:19 2000



From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Thu, 21 Dec 2000 10:41:22 GMT+1200
Subject: video camera

Contents Retrieved from Microscopy Listserver Archives
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Hi again

I want to cheaply mount an inexpensive video camera onto the optical
microscope of, yes, you guessed it, a JEOL 840. Economically, you
might say.

Somebody been there, or near there?

I'm not very knowledgeable about optics, do I need to get

1 a lensless video camera and take off the microscope eyepiece,

2 a lensless video camera and retain the microcope eyepiece,

3 a video camera with a lens and take off the microscope eyepiece,

4 a video camera with a lens and retain the microscope eyepiece,

or

5 none of the above?


I'm sure I can devise the neccessary spacer/adaptor

tia

rtch

Ritchie Sims Phone : 64 9 3737599 ext 7713
Department of Geology Fax : 64 9 3737435
The University of Auckland email : r.sims-at-auckland.ac.nz
Private Bag 92019
Auckland
New Zealand



From daemon Wed Dec 20 16:00:29 2000



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Wed, 20 Dec 2000 15:53:54 -0600
Subject: lab design book

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Scott,

One volume is "Design of the Electron Microscope Laboratory", by Ronald
Alderson. It is part of the Glauert series, "Practical Methods in Electron
Microscopy". The edition we have is from 1975 and I don't know if there is
a newer one.

Good luck,
Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/




-----Original Message-----
} From: Scott Whittaker [mailto:Whittaker.scott-at-nmnh.si.edu]
Sent: Wednesday, December 20, 2000 3:08 PM
To: Microscopy-at-sparc5.microscopy.com


I get a rare opportunity to renovate some space to move our EM suite and
have to turn in the ideal lab concept very soon. I know there is a book on
this very thing. Anyone recall the title and author??




From daemon Wed Dec 20 16:31:48 2000



From: Ekstrom, Harry :      harry.ekstrom-at-honeywell.com
Date: Wed, 20 Dec 2000 12:50:08 -0700
Subject: RE: Electron microscope first demonstration

Contents Retrieved from Microscopy Listserver Archives
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Well Kids,

I think the point we are missing here is geinfam-at-aol.com asked when the
first EM microscope was first "demonstrated". My source states the
theoretical "description" of the SEM was brought to us by H. Stintzing in
1929. However the first "demonstration" of an SEM was in 1935 by M. Knoll.
In 1932, the first TEM was "constructed" by Knoll and Ruska. I wonder how
they all felt about the electoral college.

Harry Ekstrom
Materials Laboratory

(602) 231-2744
e-mail: harry.ekstrom-at-honeywell.com





From daemon Wed Dec 20 16:46:09 2000



From: Chris Jeffree :      cjeffree-at-srv0.bio.ed.ac.uk
Date: Thu, 21 Dec 2000 00:34:02 -0000
Subject: Re: Reposting/ User Fee's

Contents Retrieved from Microscopy Listserver Archives
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Go to this site for some interesting info.

http://ernst.ruska.de/daten_e/mainframe_e.html

Barry
EMU
UNSW


----- Original Message -----
} From: Fortner, Jeffrey A. {fortner-at-cmt.anl.gov}
To: 'Microscopy Listserver' {Microscopy-at-sparc5.microscopy.com}
Sent: Thursday, December 21, 2000 3:32 AM


I worked out the real, full-costs-recovery cost of our emlab a few
years ago, and came to the conclusion that we would have to
charge around £1250 a day to our users for access to a TEM. The
charge for basic access to a top-grade analytical FEG TEM at a
commercial rate could easily be in the order of £3000 per day. The
task for most of us, as you indicate, is to keep the running costs
down to a level which the University can tolerate. Most University
facilities I know of in the UK are free of the requirement to earn the
replacement cost of the equipment, and have to earn only limited
staff costs. I am aware that there is ever-increasing pressure on
Universities to earn more of the costs of their operations. The latest
rumour is that electricity and water charges are just around the
corner, but that one is at least a decade old now. I'll believe it when
they install the meters. Maybe it'll come, maybe it won't. But in the
past 15 years of operating a facility I swear that the user attitudes to
charging don't reflect any recognition of this. Internal users still
lobby for access to be free, or at least to appear free at the point of
use. We therefore make just a few k per annum towards our costs
by selling a little slack time to any local industries that have a need
for our services. Who exactly are we cheating by doing this? An
alternative way of looking at it is that we are offering the public some
access to a facility they paid for in the first place, at a rate which is
no more than is necessary to keep the machines running. I
personally don't know of any commercial operation anywhere within
hundreds of miles of Edinburgh that we are in competition with.
Our operation is strictly self-limiting. once we have made the few k
required to cover our costs the focus is back on internal user
support. If there is any real industrial EM business out there any
competent commercial operation would have no difficulty whatever
in surviving whatever level of pseudo-commercial activity Universities
are involved in.
If there is any real evidence to the contrary I would like to hear it
(and would be amazed)
We're no threat, so please don't keep shooting us!
Chris


} The point of this is that nearly all university facilities will eventually

} have to be accountable for all of their expenses and adjust their rates to
} cover the costs. But universities still will have an advantage in that
} they usually don't have to purchase the principle equipment from operating
} expenses. How NSF granted instrumentation can be used is described in NSF
} Important Rule 91.
}
} When university labs have to account for all of their operating expenses,
} they will become competitors of private non-subsidized labs. If they offer
} instruments rates less than private lab, they are cheating.
}
} John Humenansky/Staff Scientist
} Physical Electronics, Inc. (PHI)
} 6509 Flying Cloud Drive
} Eden Prairie, MN 55344
} 952-828-6387
}
}


~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Dr Chris Jeffree
University of Edinburgh
Biological Sciences EM Facility
Daniel Rutherford Building
King's Buildings EDINBURGH EH9 3JH
Tel: +44 (0) 131 650 5345
FAX: +44 (0) 131 650 6563

Inveresk Cottage, 26 Carberry Road,
Inveresk, Musselburgh, Midlothian EH21 8PR, UK
Tel: +44 (0) 131 665 6062 / Mobile 07710 585 401
FAX: +44 (0) 131 653 6248
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~



From daemon Wed Dec 20 19:40:24 2000



From: =?ks_c_5601-1987?B?Sm9uZG8gWXVuIMCxwbi1tQ==?= :      jdyun-at-kyungnam.ac.kr
Date: Thu, 21 Dec 2000 10:23:04 +0900
Subject: Thickness measurement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Listers:

Can anybody suggest how to measure thickness of the conductive coating for EM specimen? If it is transparent, ellipsometer could be used. Since metallic or carbon coating is not transparent, I could not use that method. Then do I have only way to measure directly, like measuring thickness on the cross-section or on the surface by using EM or SPM? Is there any other way?

Jondo Yun
jdyun-at-kyungnam.ac.kr



From daemon Wed Dec 20 20:37:38 2000



From: Joon Hwan CHOI :      jchoi-at-engineering.ucsb.edu
Date: Wed, 20 Dec 2000 18:32:22 -0800
Subject: TEM specimen preparation of titania particles

Contents Retrieved from Microscopy Listserver Archives
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Dear Collegues,

I have the following problem during my TEM observation.
I am looking at titania (TiO2), rutile, particles which are placed on top of a carbon coated cooper grid. Those particles are ca. 90 nm long and ca. 15 nm wide. At 200 kV the particles are very unstable under the electron beam. I think charging occurs. The attempt to coat the sample with carbon did show some success, but even after very long sputtering times (15 min. at 25 A) most particles twinkle.

If anybody has an idea for me how to avoid the twinkling, preferentially without sputtering any carbon on top of the sample, I would be very thankful.

Sincerely,
Joon

************************************************************
Joon Hwan CHOI, Ph.D.
Post-doctoral Researcher
Materials Department
University of California at Santa Barbara (UCSB)
Santa Barbara, CA 93106

Mailto: jchoi-at-engineering.ucsb.edu / joonhwanchoi-at-netzero.net

Office Phone (805) 893-2008 / Fax (805) 893-8971, 8486
************************************************************



From daemon Wed Dec 20 21:09:37 2000



From: Walck, Scott D. :      walck-at-ppg.com
Date: Thu, 21 Dec 2000 01:52:04 -0500
Subject: RE: TEM specimen preparation of titania particles

Contents Retrieved from Microscopy Listserver Archives
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Ernst Ruska received in 1986 the Nobel Prize in Physics "for his fundamental
work on electron optics and for the design of the first electon microscope".

You can read more on the Official Web Site of the Nobel Foundation:
http://www.nobel.se/physics/laureates/1986/index.html

Cheers,

Paul
======================
Paul Baggethun
Alcoa Technical Center
Alcoa Center, PA 15069
USA
======================

-----Original Message-----
} From: "Geinfam-at-aol.com"-at-sparc5.microscopy.com
[mailto:"Geinfam-at-aol.com"-at-sparc5.microscopy.com]
Sent: Tuesday, December 19, 2000 6:36 PM
To: Microscopy-at-sparc5.microscopy.com


I think the twinkling problem is more electron beam heating of your sample as opposed to charging. If the samples were charging, they would be moving on the carbon support film. Carbon coating the samples will help. Are you using holey carbon grids or continuous support films. The latter should help somewhat, but you have about 200A of carbon that adds to the thickness. You could also try a cold stage to see if that could help. You could also try a higher voltage machine if one is available to you. Higher voltages allow the electrons to pass through your sample with less interaction and therefore less heating occurs.


-Scott

Scott D. Walck, Ph.D.
PPG Industries, Inc.
Glass Technology Center
Guys Run Rd. (packages)
P. O. Box 11472 (letters)
Pittsburgh, PA 15238-0472

Walck-at-PPG.com

(412) 820-8651 (office)
(412) 820-8161 (fax)



-----Original Message-----
From: Joon Hwan CHOI [mailto:jchoi-at-engineering.ucsb.edu]
Sent: Wednesday, December 20, 2000 9:32 PM
To: Microscopy-at-sparc5.microscopy.com
Subject: TEM specimen preparation of titania particles


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Dear Collegues,

I have the following problem during my TEM observation.
I am looking at titania (TiO2), rutile, particles which are
placed on top of a carbon coated cooper grid. Those particles
are ca. 90 nm long and ca. 15 nm wide. At 200 kV the particles
are very unstable under the electron beam. I think charging
occurs. The attempt to coat the sample with carbon did show
some success, but even after very long sputtering times (15
min. at 25 A) most particles twinkle.

If anybody has an idea for me how to avoid the twinkling,
preferentially without sputtering any carbon on top of the
sample, I would be very thankful.

Sincerely,
Joon

************************************************************
Joon Hwan CHOI, Ph.D.
Post-doctoral Researcher
Materials Department
University of California at Santa Barbara (UCSB)
Santa Barbara, CA 93106

Mailto: jchoi-at-engineering.ucsb.edu / joonhwanchoi-at-netzero.net

Office Phone (805) 893-2008 / Fax (805) 893-8971, 8486
************************************************************




From daemon Thu Dec 21 02:39:12 2000



From: Faerber Jacques :      Jacques.Faerber-at-ipcms.u-strasbg.fr
Date: Thu, 21 Dec 2000 09:33:56 +0100
Subject: early SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


About the firts TEM, the question is answered. But about the SEM, a few
precisions.

Some interresting papers gives the essential informations about its
history. But we must keep in mind that the early SEM was a "reflexion
microscope" designed in analogy with the optical microscope. And the
development were made in paralell with the TEM. So the design looks like a
modified TEM, and not like what we know today as a SEM. It was also the
case with the first Stereoscan.

The papers :

M. Von Ardenne
Zur Geschichte der Rasterelektronenmikroskopie und der
Elektronenmikrosonde.
Optik 50 (1978) No 3, 177-188

C.W. Oatley
The early history of the scanning electron microscope
J. Appl. Phys. 53(2) Feb 1982

The first papers of Von Ardenne were from 1937 and 39 (Zeitschrift fur
Physik), and he had built an instrument and published some photography.
The intrument was destroyed by the bombardement at the end of WW II.

An other interresting paper is :

V.K. Zworykin, J. Hillier and R.L.Snder
A Scanning Electron Microscope
ASTM bulletin August 1942

After a lot of nice experiments (with FE guns!) they conclued that it was
too expensive, with a poor signal and to less resolution.


J. Faerber
IPCMS-GSI
23, rue de Loess
67037 Strasbourg CEDEX
France

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)0 88 10 72 48
E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr




From daemon Thu Dec 21 06:28:47 2000



From: Patton, David :      David.Patton-at-uwe.ac.uk
Date: Thu, 21 Dec 2000 12:22:22 +0000 (GMT Standard Time)
Subject: Re: ESEM users

Contents Retrieved from Microscopy Listserver Archives
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We fix human cells for safety reasons but sometimes look at
them wet (hard work!). Chris Gilpin argues that fixation
helps specimens resist beam damage and that most distortion
occurs during drying rather than fixation.

Dave




On Wed, 20 Dec 2000 16:15:13 -0500 Scott Whittaker
{Whittaker.scott-at-nmnh.si.edu} wrote:

} ------------------------------------------------------------------------
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} -----------------------------------------------------------------------.
}
}
} I am now in charge of a Phillips XL30 ESEM and have a couple of questions. What are you doing to prevent contamination of the specimen chamber. I had a user wanting to view human tissue today unfixed and am reluctant to do so. Anyone have a solution. Mine was to fix and process for standard SEM but that may not be an option for some of the in-situ studies or preserved museum specimens they don't want to wash or damage. Thanks
}
}

----------------------------------------
Patton, David
Email: David.Patton-at-uwe.ac.uk
"University of the West of England"




From daemon Thu Dec 21 08:38:36 2000



From: Leona Cohen-Gould :      lcgould-at-mail.med.cornell.edu
Date: Thu, 21 Dec 2000 09:35:40 -0500
Subject: Re: lab design book

Contents Retrieved from Microscopy Listserver Archives
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At 4:08 PM -0500 12/20/00, Scott Whittaker wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

*******************
Hi Scott,
I just pulled the volume off my shelf. It is part of the series
edited by Audrey Glauert: Practical Mehods in Electron Microscopy
Volume 4 "Design of the Electron Microscope Laboratory" written by
Ronald H. Alderson original copyright 1975. There may be a more
recent edition, but this one certainly covers the main considerations.

I envy your opportunity to "start from scratch" many of us end up
retrofitting spaces designed for other purposes.Have fun.

Lee

Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175



From daemon Thu Dec 21 08:59:00 2000



From: George Langford :      amenex-at-amenex.com
Date: Thu, 21 Dec 2000 10:01:38 -0500
Subject: Re: User fee discussion

Contents Retrieved from Microscopy Listserver Archives
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Hello Microscopists !

By analogy, any combination (even among nonprofits) for the purposes
of setting rates must be illegal if the well publicized divvying up
of applicants to graduate school was an illegal restraint of trade.

Private for-profit industry was not involved in that particular
conspiracy in any way.

On the other hand, the mere listing of categories of expense and
the discussion of how best to allocate those costs between users
and providers seem appropriate. That's essentially the same as
a discussion of the principles of accounting or the geometry
of the Ewald sphere.

When specific rates are mentioned, that's what looks like price
fixing.

Best regards,
George Langford, Sc.D.
amenex-at-amenex.com



From daemon Thu Dec 21 09:12:53 2000



From: Valerie Woodward :      WOODWARD-at-brk.bfg.com
Date: Thu, 21 Dec 2000 10:04:06 -0500
Subject: fluorescence euipment needed

Contents Retrieved from Microscopy Listserver Archives
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Greetings all:

I am looking for a pol nosepiece for a Leitz Ortholux 2 microscope. I welcome any responses, off-list please, to the e-mail listed below.

Thanks,


Valerie P. Woodward
Senior R&D Chemist
BFGoodrich Performance Materials
Measurement Science, D/2197
Microscopy and X-ray Analysis
9921 Brecksville Rd.
Brecksville OH 44141-3289
(216) 447-5408 (voice)
(216) 447-5575 (FAX)
woodward-at-brk.bfg.com (e-mail)




From daemon Thu Dec 21 10:15:34 2000



From: Tom Phillips :      PhillipsT-at-missouri.edu
Date: Thu, 21 Dec 2000 09:53:28 -0600
Subject: user fees

Contents Retrieved from Microscopy Listserver Archives
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I disagree that posting rates implies core facilities are attempting
price-fixing. Surely commercial entities look at the published costs
of their competitors all the time. When one airline drops its rates,
the others usually follow within 24 hrs. Price-fixing only results
if you make your decisions in concert with your competitors. Knowing
how much other core facilities charge has several benefits. First,
the core knows what level of fees allow investigators to afford to
use a technology. If I charged enough to fully reimburse the true
expenses of my core, most of my researchers would simply switch to
alternative techniques since they have limited grant dollars.
Secondly, it allows core directors to demonstrate to administrators
what portion of operating expenses other cores attempt to recover
from user fees. I always tell my administrators that I am to make a
profit that rivals our university library! Lots of cores say they try
to recover operating expenses but unlike industry, we never account
for building costs, electricity and heat, maintenance, etc.
Researchers pay for those types of things with their indirect costs
that accompany virtually all grants. This makes a comparison of
commercial vs university fees impossible to judge. Indirect costs
also help many universities pay for the "matching portion" that most
federal grant agencies require as a part of matching equipment
grants. Whether I use the flow cytometer on my campus or not, my
indirect costs help pay for it. In addition, as a tax-paying
citizen of the state of Missouri, my tax dollars help subsidize our
University. We pay these taxes, in part, because we want our
students to have access to the cutting edge technologies that will
make them competitive citizens. What good would having all these fun
toys be if they were too expensive to allow our undergrads and grads
some time on them. These subsidies have no equivalent with private
industry.

Anyone wanting to see what MU charges for confocal and other types of
LM, should surf over to
http://www.biotech.missouri.edu/mcc/prices.html . The costs for our
EM core are at http://www.biotech.missouri.edu/mbp/cores/ . I am
responsible for posting the LM core prices but Randy Tindall is
responsible for the EM core prices being posted so please direct all
lawsuits regarding them at him (Sorry, Randy). See you in court.

Merry Christmas and Happy Holidays to all. Tom




--
Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility

3 Tucker Hall
Division of Biological Sciences
University of Missouri
Columbia, MO 65211-7400
(573)-882-4712 (voice)
(573)-882-0123 (fax)



From daemon Thu Dec 21 10:19:31 2000



From: Brian W Robertson :      brobertson-at-unl.edu
Date: Thu, 21 Dec 2000 10:16:07 -0600
Subject: lab space

Contents Retrieved from Microscopy Listserver Archives
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Dear Scott
I had the same opportunity to design for an EM lab when I moved to the
University of Nebraska -- new facility, new space. Fortunately the lab
spaces offered included one that I checked to be virtually ideal. This was
in the basement of a building that had 3 floors above ground plus the
basement and that was intended to be suitable for adding another 3 floors
on top. The footings and the (1 foot thick reinforced concrete) wall
surrounding the lab were really massive and confer the best stability I've
heard of -- floor vibration velocities { 0.5micrometers per second (at
frequencies 1 to } 50 Hz) and amplitudes { 0.5 micrometers (peak to peak)
and AC magnetic fields {0.5 mG (before EM equipment installation). I got
the architectural and construction team to provide several important
provisions for use of the space for EM:
1. laminar air inlets to rooms (positioned so as not to affect the TEM
columns!),
2. near-floor level return air vent in the TEM rooms (to satisfy needs to
remove SF6 since it is denser than air),
3. acoustic lining of air inlet ducts,
4. temperature regulation to +/- 1 C with a maximum rate 1 C / hour,
5. humidity regulation in the range 40 to 60% relative humidity year round
6. water chillers in a mechanical facilities room adjacent to, but outside,
the EM facility.

The above aspects overlap and go beyond the necessarily somewhat outdated
recommendations in Anderson's book.

There were still issues that required care in order for the TEM to meet the
image drift specifications I required of the manufacturers. These measures
included adjustment of the air flow in the HREM room and reduction of the
temperature difference between the TEM water temperature and the room air
temperature. The TEM water chiller also required to be equipped with the
precision electronic temperature regulation option. Under these conditions,
there is no need to install curtains or otherwise to curtail air
circulation during high resolution work.

I am very happy that our facilities design and management staff,
construction and maintenance people, and microscope manufacturer's
representatives could all be persuaded to achieve the desired end result --
continued, essentially trouble-free, stable operations of our lab in all
conditions of weather and use of the rest of the building. It is definitely
worth taking great care over the major and minor details before you start!

If I can provide other information. please let me know.
Best wishes
Brian Robertson

***********************************************************
Assoc. Prof. Brian W. Robertson
Department of Mechanical Engineering
and Center for Materials Research and Analysis
University of Nebraska-Lincoln, N124 WSEC,
17th & Vine Sts., Lincoln, NE 68588-0656, USA
** Office 402 472 8308; FAX 472 1465; Labs 472 8762 and 472 5498 **



From daemon Thu Dec 21 10:24:22 2000



From: Bruce Brinson :      brinson-at-rice.edu
Date: Thu, 21 Dec 2000 10:22:46 -0600
Subject: Re: video camera

Contents Retrieved from Microscopy Listserver Archives
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HI Ritchie,
here is my cheap fix ( {1K$US)
As you mention in 1-4 the eye piece is the trick. If you have std 23 mm
eye pieces (For larger slip in eye pieces you can make a bushing) you can
buy a relay lens from Edmund Scientific & others (23 mm x c-mount). Then buy
a color security camera w/c-mount & connect to your video recording device.
I use a Snappy TM interface to my laptop. I can throw all this in my laptop
tote sack & aquire pretty decent images at almost any optical u-scope I walk
up to.
ball park on economics, optics $500, camera $200-300, Snappy {$100US
For higher res. images there has been a lot of buzz on this list server
about interfacing the Cool Pic TM digital camera. ck the archives.
Don't forget to recalibrate you magnification.

If you have thread on eye pieces... I'd like to know what you find out.

disclaimer: no financial interest in companies or products mentioned.

Happy holidays,
Bruce Brinson
Rice U.

Ritchie Sims wrote:

} ------------------------------------------------------------------------
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} Hi again
}
} I want to cheaply mount an inexpensive video camera onto the optical
} microscope of, yes, you guessed it, a JEOL 840. Economically, you
} might say.
}
} Somebody been there, or near there?
}
} I'm not very knowledgeable about optics, do I need to get
}
} 1 a lensless video camera and take off the microscope eyepiece,
}
} 2 a lensless video camera and retain the microcope eyepiece,
}
} 3 a video camera with a lens and take off the microscope eyepiece,
}
} 4 a video camera with a lens and retain the microscope eyepiece,
}
} or
}
} 5 none of the above?
}
} I'm sure I can devise the neccessary spacer/adaptor
}
} tia
}
} rtch
}
} Ritchie Sims Phone : 64 9 3737599 ext 7713
} Department of Geology Fax : 64 9 3737435
} The University of Auckland email : r.sims-at-auckland.ac.nz
} Private Bag 92019
} Auckland
} New Zealand




From daemon Thu Dec 21 10:49:26 2000



From: Tindall, Randy D. :      TindallR-at-missouri.edu
Date: Thu, 21 Dec 2000 10:38:45 -0600
Subject: Rates

Contents Retrieved from Microscopy Listserver Archives
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Tell you what, folks. Next time we marauding academic pirates are in need
of guidance on fees, let's just ask for links to web pages where other labs
already have their rates published. Maybe that will make everybody happy
and serve the same purpose as our usual blatant conspiracy does. That way
we can take over the whole world legally!

Yeah, that's the ticket.....

Randy

Randy Tindall
EM Specialist
Electron Microscopy Core Facility
W122 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-5414
Email: tindallr-at-missouri.edu
Web: http://www.biotech.missouri.edu/emc/






From daemon Thu Dec 21 10:51:12 2000



From: Faerber Jacques :      Jacques.Faerber-at-ipcms.u-strasbg.fr
Date: Thu, 21 Dec 2000 17:47:24 +0100
Subject: LaB6 filament problem

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Hello

Has some a recept to clean the ceramic holder from a LaB6 filament. It is
shorted (300 kohm at rohm temp., 0 at working temp.) with the filament.
The tip is OK. Is it a way to remove the evaported coating (LaB6,
decomposed LaB6 or carbon ?) on the ceramic, without dammaging the tip.
Mechanically seems to bee inpracticable, it's too small (10 mm diameter, 5
mm heigth between the ceramic holder and the graphite blocs), and I have
no "microbillage" (I don't know in english). But chemicaly ? I don't know
the manufacturer of the tip, it comes from a LEED optic.

Thanks

J. Faerber
IPCMS-GSI
23, rue de Loess
67037 Strasbourg CEDEX
France

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)0 88 10 72 48
E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr




From daemon Thu Dec 21 10:52:53 2000



From: JHumenansky-at-phi.com
Date: Thu, 21 Dec 2000 10:49:05 -0600
Subject: Re: User Fee's

Contents Retrieved from Microscopy Listserver Archives
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Your reply proves my point. If you were a business your personnel salaries
and instrument costs would have to be factored into the ACTUAL operating
costs. The instrument rates that you charge for outside clients are based
on YOUR operating costs which do not include salaries or instruments and
can therefore be much lower than what a business MUST charge to recover
their costs and make a profit. Many universities are not in competition to
private business because they are funded internally and exist primarily for
education and research. Too often however the economics of operating a
university facility collide with the reduction in institutional funding and
then outside users are sought which is the unfair competition aspect unless
the university rates for outside users are the same as what the businesses
are charging for similar equipment.

John Humenansky/Staff Scientist
Physical Electronics, Inc. (PHI)
6509 Flying Cloud Drive
Eden Prairie, MN 55344
952-828-6387




From daemon Thu Dec 21 11:49:40 2000



From: Marie E. Cantino :      cantino-at-uconnvm.uconn.edu
Date: Thu, 21 Dec 2000 12:46:27 -0500
Subject: Re: user fees

Contents Retrieved from Microscopy Listserver Archives
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Tom Phillips wrote:

} We pay these taxes, in part, because we want our
} students to have access to the cutting edge technologies that will
} make them competitive citizens. What good would having all these fun
} toys be if they were too expensive to allow our undergrads and grads
} some time on them.

I would also argue that students who never have a chance to see or use an
electron microscope during their training are less likely to seek out those
services later when they go to work in the private sector. I strongly
suspect that the availability of EM in Universities is an important factor
in creating a market for private sector EM providers.

Marie

Dr. Marie E. Cantino
Dept. of Physiology and Neurobiology, U-2131
University of Connecticut
Storrs, CT 06269-2131
Phone: 860-486-3588
Fax: 860-486-6369





From daemon Thu Dec 21 14:09:26 2000



From: Greg Erdos :      gwe-at-biotech.ufl.edu
Date: Thu, 21 Dec 2000 15:05:46 -0500
Subject: Fwd: Re: User fee discussion

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}
}
} One point that seems to have been missed is that the various
} university labs do not compete with each other but rather need to recover
} costs within their institution. This is a cost recovery and
} redistribution process from within a single fiscal entity. (All grants
} etc. are university funds and no longer belong to the granting agency or
} the PI.) So I don't get this restraint of trade nonsense. We do not
} sell anything within our institution. We provide a service that must be
} financially supported in one way or another and it is most appropriate to
} have the users of our facility provide all or part of those costs. So I
} figure we can discuss anything we damn well please among professionals
} who provide these services.
}
} At 10:01 AM 12/21/2000 -0500, you wrote:
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Gregory W. Erdos, Ph.D.
Assistant Director, Biotechnology Program
PO Box 110580
University of Florida
Gainesville, FL 32611

Ph. 352-392-1295 Fax: 352-846-0251



From daemon Thu Dec 21 18:05:52 2000



From: Nestor J. Zaluzec :      zaluzec-at-microscopy.com
Date: Thu, 21 Dec 2000 17:59:10 -0600
Subject: Re: Administrivia: User Fee's

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Colleagues....

I think all opinions have been aired and nothing new
has been said for awhile. I'm going to suggest that the
thread be left to ferment for another year when I'm sure
the question will get asked once again.

Nestor
Your Friendly Neighborhood SysOp








From daemon Thu Dec 21 18:37:38 2000



From: John F. Turner :      j.f.turner-at-csuohio.edu
Date: Thu, 21 Dec 2000 19:38:03 -0600
Subject: Re: video camera

Contents Retrieved from Microscopy Listserver Archives
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Ritch,

It's a good bet that it will work. If it doesn't, try changing the
distance between the microscope and the camera. Also, if you cannot
fill the video chip completely with the image, or if it is over filled
or distorted, try using the video lens with an eyepiece preceding it
before you consider purchasing any additional optics. The alignment of
the eyepiece and video lens from side to side, as well as the distance
separation them are critical. A small deviation can lead to large
changes in the image appearance and quality. There is a sweet spot, so
patience prevails. Again, this is not the optimal way to interface a
video camera and a microscope, but it is cost effective and performs
well in many non-critical applications. If the video lens has an
adjustable focus, it may be possible to use it without the eyepiece, and
it is worth a try if all else fails.

Best of luck

John


John Turner, Ph.D.
Director, Advanced Chemical Imaging Facility
Cleveland State University
Cleveland, Ohio 44115





From daemon Thu Dec 21 21:37:09 2000



From: Ken Converse :      qualityimages-at-netrax.net
Date: Thu, 21 Dec 2000 23:26:32 -0800
Subject: Re: Electron microscope first demonstration

Contents Retrieved from Microscopy Listserver Archives
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I beleive the first high resolution SEM was built by Oliver Wells as a PhD project
in 1959. It was enabled by the Everhart-Thornley secondary electron detector. My
understanding is that the theory of how an SEM should work was developed in the
'30s but specimen current and other available signals couldn't be amplified
sufficiently or cleanly enough to be useable. Most of them still can't be used for
high resolution work.

Ken Converse
owner
Quality Images
third party SEM service
Delta, PA

Jim at ProSciTech wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
} In 1935? That was a TEM, not an SEM. Knoll was Ernst Ruska's PhD supervisor and
} later collaborator. They designed and built the first TEMs.
} The very first model had no specimen chamber and just proved that the lens
} system could work. So the first model, which I seem to remember was before 1935
} was really not a microscope.
} Von Ardenne described the operating principle of an SEM in the late 30th, but I
} believe none was build until the early 60th at Cambridge University, UK.
} Cheers
} Jim Darley
} ProSciTech Microscopy PLUS
} PO Box 111, Thuringowa QLD 4817 Australia
} Ph +61 7 4774 0370 Fax:+61 7 4789 2313 service-at-proscitech.com
} Great microscopy catalogue, 500 Links, MSDS, User Notes
} ABN: 99 724 136 560 www.proscitech.com
}
} On Wednesday, December 20, 2000 1:57 PM, Ekstrom, Harry
} [SMTP:harry.ekstrom-at-honeywell.com] wrote:
} }
} }
} } My source quotes M.Knoll first demonstrated the SEM in 1935 but no specific
} } date.
} }
} } Harry Ekstrom
} } Materials Laboratory
} }
} } (602) 231-2744
} } e-mail: harry.ekstrom-at-honeywell.com
} }
} }
} } -----Original Message-----
} } } From: "Geinfam-at-aol.com"-at-sparc5.microscopy.com
} } [mailto:"Geinfam-at-aol.com"-at-sparc5.microscopy.com]
} } Sent: Tuesday, December 19, 2000 4:36 PM
} } To: Microscopy-at-sparc5.microscopy.com
} } Subject: Electron microscope first demonstration
} }
} }
} } ------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} } On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} }
} } Can anyone tell me the exact date in the "30's when the electron microscope
} } was first demonstrated?
} }
} } geinfam-at-aol.com
} }




From daemon Fri Dec 22 08:38:36 2000



From: Jean-Claude Lebosse :      Jean-Claude.Lebosse-at-insa-lyon.fr
Date: Fri, 22 Dec 2000 15:48:39 -0500
Subject: Use of ICXANES

Contents Retrieved from Microscopy Listserver Archives
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Dear listmembers,

Is there somebody who know how to calculate fine structures near L23 edges by using
ICXANES, while this code is restricted to spectra involving core levels of s symmetry,
i.e. K, L1...?

Best regards.

JC Le Bossé and R Chassagnon



From daemon Fri Dec 22 08:41:22 2000



From: Dr. Volker Brinkmann :      brinkmann-at-mpiib-berlin.mpg.de
Date: Fri, 22 Dec 2000 15:38:34 +0100
Subject: unsubscribe

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From daemon Fri Dec 22 09:08:48 2000



From: Zhenquan Liu :      zhenquan.liu-at-asu.edu
Date: Fri, 22 Dec 2000 08:04:45 -0700
Subject: About tem specimen

Contents Retrieved from Microscopy Listserver Archives
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Dear Erdem,

Please tell us some details about your email. Do you have a sample to
prepare? Or you just want to know the general knowledge?

Cheers!

Zhenquan Liu

---------------------------------------------------------------------------

Dear Sir/Madam,
Can anybody tell me how to prepare TEM samples for Single crystal?
Thanks for your interested




**********************************
**********************************
** Research.Asst.ERDEM YASAR **
** University of Kirikkale **
** Department of Physics **
** TEM LABORATORY **
** 71450 KIRIKKALE/TURKEY **
** erdem.yasar-at-physics.org **
** yasar-at-science.ankara.edu.tr **
** yasar-at-turkuaz.kku.edu.tr **
**********************************
**********************************



------------------------------------
Zhenquan Liu (Dr.)
Arizona State University
CSSS, CHEM
Room PSA213,
Tempe, AZ 85287
Tel (480) 965 4544 (o)
(480) 775 7428 (h)
Fax (480) 965 9004 (o)
Email zhenquan.liu-at-asu.edu
------------------------------------





From daemon Fri Dec 22 10:40:47 2000



From: michael shaffer :      epmalab-at-darkwing.uoregon.edu
Date: Fri, 22 Dec 2000 08:36:49 -0800
Subject: RE: SEM: mystery CL

Contents Retrieved from Microscopy Listserver Archives
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Warren writes ...

} Are you going to fill us in on the answer, or do you even know it?
} ...
} Inquiring minds want to know.

I thought I had ... it is a mystery to me how some posts, for example
this facility supervisor vacancy's announcement, get cross-posted
everywhere, and why the subsequent discussion of this image missed
this list :o)

http://epmalab.uoregon.edu/images/zircon-xmas.jpg

It is an image of e-beam induced CL as acquired via RGB filters. The
blue is somewhat typical of igneous (or primary) quartz ... the red
halo is evidence of radiation damage from a nearby zircon inclusion.
Other images from the same rock actually show us the "smoking gun".
We investigated a number of sandstones, and otherwize quartz bearing
rocks from this environment, and another type of radiation induced CL
we observed was red indistinct rims on qtz grains in sandstone. We
imagined radioactive fluids percolating through the rocks.


happy holidays ... shAf:o)




From daemon Fri Dec 22 11:17:32 2000



From: Ingber, Bruce F. :      bingber-at-commserver.srrc.usda.gov
Date: Fri, 22 Dec 2000 11:18:34 -0600
Subject: RE: LN2 and the Amazing Exploding Funnel

Contents Retrieved from Microscopy Listserver Archives
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My turn for True Confessions or surviving liquid nitrogen incidents.

Many years ago in another time warp (70's) a "green" technician (no, not
environmental green) was dutifully following his supervisor's orders to fill
EDX detector Dewars with liquid nitrogen. The staff had used a heavy-duty
plastic Nalgene pitcher, wrapped with lab tape, to accomplish this chore.
Thus, the technician followed protocol and used the same pitcher to collect
the cryogenic fluid. And it worked well for many, many weeks. However, one
day as he was filling the wrapped container, there was an enormous explosion
(or implosion?). The technician was left holding the handle of the pitcher
with the rest of the pitcher vanished into hundreds of small plastic pieces
and liquid nitrogen splattered everywhere. Holy mackerel! But for the grace
of God, not one piece of plastic or drop of liquid nitrogen hit the
SURPRISED technician!

However, to this day that technician remembers his mother's admonitions
about wearing clean underwear, you never know when an eighteen wheeler will
hit you.

Bruce F. Ingber, Biologist
USDA-ARS, SRRC
1100 Robert E. Lee Blvd.
New Orleans, LA 70124
(504) 286-4270 phone
(504) 286-4419 fax
bingber-at-commserver.srrc.usda.gov





From daemon Fri Dec 22 11:38:02 2000



From: =?ISO-8859-1?Q?Laboratorio_de_Microscop=EDa_-_UNER?= :      microsc-at-fi.uner.edu.ar
Date: Fri, 22 Dec 2000 13:38:39 -0000
Subject: unsuscribe

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===================================================
Fernando D. Balducci
Laboratorio de Microscopia Electrónica
Facultad de Ingeniería - Bioingenieria
Universidad Nacional de Entre Rios
Argentina
e-mail: microsc-at-fi.uner.edu.ar
tel: 54 43 975100
fax: 54 43 975077


===================================================



From daemon Mon Dec 25 17:22:49 2000



From: service-at-promoteacasino.com
Date: 25 Dec 2000 19:57:31 -0500
Subject: Promote a casino

Contents Retrieved from Microscopy Listserver Archives
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Dear Internet Marketer,

Are you in the bulk email/internet marketing business and want to make some serious $$$ by owning your own ONLINE CASINO!! Check out this latest HOT HOT concept at www.promoteacasino.com

Sincerely,

Promote a casino INC

P.S. You have been contacted because your company/you were listed as being in the internet marketing business. If you don't reply back, YOU WILL NEVER BE CONTACTED AGAIN.



From daemon Tue Dec 26 07:36:47 2000



From: Beth Bray :      bbray-at-sc.rr.com
Date: Tue, 26 Dec 2000 19:49:18 -0500
Subject: RE: preparation TEM sample of coral

Contents Retrieved from Microscopy Listserver Archives
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Hi, all
Thanks for your attention to my question on coral TEM specimen. The coral
is hard, but not dense. A lot of platelets arranged in radiation way were
observed in optical microscope. Can anybody give me some suggestion to
prepare it?
Thanks again.
**************************************************
Dr. Feng Wu
Dept. of Materials Engineering
Ben-Gurion University of the Negev
Beer-Sheva 84105, Israel

fax 972-7-6472944
tel 972-7-6461473(o)
-6281432(h)
email: fwu-at-bgumail.bgu.ac.il
************************************************
----- Original Message -----
} From: Feng Wu {fwu-at-bgumail.bgu.ac.il}
To: {Microscopy-at-sparc5.microscopy.com}
Sent: Wednesday, December 20, 2000 10:34 AM


Dear Dr. Feng Wu,

I have used the following procedure to fix specimen of Leptogorgia
virgulata, an octocoral (sea whip) found off the eastern coast of the USA.
When fixed according to this procedure, whole tissue specimen, as well as
the harvested sclerites by themselves, can be prepared for viewing in either
SEM or TEM. I hope you find this information helpful.

Regards,

Elizabeth Bray
Plant Chemist
SCE&G Central Laboratory
2102 N. Lake Dr.
Columbia, SC 29212
USA


Fixing and Embedding Protocol for Octocorals and Their Sclerites
================================================================

Reagent Preparation:
1. Purchase the following:
16% Paraformaldehyde (PFA)
50% Gluteraldehyde (GA)
NaCacodylate, reagent grade [Sodium cacodylate (CH3)2AsO2Na · 3H2O, MW =
214.02]
LR White acrylic embedding medium

2. 0.1 M NaCacodylate (CH3)2AsO2Na · 3H2O, MW = 214.02
21.4 g NaCacodylate diluted to 1000 mL with DW. Adjust the pH to 7.4 with
4% NaOH or 1:10 HCl.

3. Fixative: 2% PFA, 0.15% GA, 0.05 M NaCacodylate in filtered Sea Water:
6.3 mL of 16% PFA
0.15 mL of 50% GA
25.0 mL of 0.1 M NaCacodylate, pH = 7.4
18.55 mL of Millipore filtered Sea Water
Total Volume of fixative = 50 mL

Procedure:
1. Fix the specimen in PFA/GA/NaCaco fixative for 1 hour.
2. Rinse in 0.05 M NaCaco Buffer 3 x 10 minutes.
3. Dehydrate in a series of EtOH (ethyl alcohol) washes of ever increasing
strength:
50% EtOH 10 minutes.
75% EtOH 10 minutes.
95% EtOH 15 minutes.
100% EtOH 15 minutes x 2, done under vacuum.

4. Three (3) changes of LR White, all done under vacuum.
The first two changes for at least 30 minutes, and the last change
overnight.
The last change should be done in the BEEM capsules in which the specimen
will be cured.

NOTE: Be sure that when you fill the capsules with the LR White for the
final time, there are no air bubbles in them. This will cause the level of
embedding medium to become lower in the capsule as it sits overnight under
vacuum, and when you cap them for curing in the oven, the air inside will
lead to incomplete curing making it difficult, if not impossible, to
properly section the hard coral material. To ensure that there are no
bubbles present, allow the capsules to sit under vacuum for 30 minutes to 1
hour, go back and top off any capsules that need it, then reestablish the
vacuum and allow them to sit overnight. Before capping, ensure that the
level of embedding medium is at the top of the capsule. If it is not,
carefully add sufficient medium to restore the proper level and proceed with
capping.

5. Cap to ensure the exclusion of air and cure overnight in a 60o C oven.




From daemon Wed Dec 27 05:40:24 2000



From: Christensen, Kim :      ChristeK-at-whiteoaksemi.com
Date: Wed, 27 Dec 2000 06:31:47 -0500
Subject: TEM: Job Opening

Contents Retrieved from Microscopy Listserver Archives
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A position as a TEM Engineer is open at Infineon Technologies, Richmond.

Experience Requirements: The candidate should have at least a BS degree and
1 or more years of TEM analysis experience. They should be able to work in a
team environment, possess good written and verbal skills and excel at
problem solving. Additionally, they should understand the fundamental tools,
techniques and failure mechanisms associated with TEM analysis of
semiconductors and be able to assist technicians in planning and performing
the analysis.

Job Responsibilities: To coordinate, optimize and perform TEM analysis in
support of DRAM fabrication.

The candidate will perform:
1: Coordination of TEM analysis jobs with lab customers and TEM
technicians
2: Compilation, documentation and presentation of analysis results.
3: Coordination of tool maintenance and development.
4: TEM sample preparation using mechanical polishing and focused ion
beam (FIB) techniques.
5: TEM inspections of CMOS semiconductor interfaces, dislocations,
films and defects.

About Infineon Technologies Richmond: Infineon Technologies' Richmond is
situated on 210 acres in the White Oak Technology Park, 14 miles east of
downtown Richmond, Virginia in the eastern part of Henrico County. Infineon
Technologies Richmond is just four miles east of the Richmond International
Airport, with easy access to major interstate highways. The Atlantic Ocean,
Washington, D.C. and the Blue Ridge Mountains are all within a two-hour
driving radius. We are a fully integrated semiconductor manufacturer using
cutting-edge equipment and technology. Our facilities include a
state-of-the-art wafer fab, probe, test, assembly and module manufacturing
operations. We were Semiconductor International Magazine's choice for "Top
Fab of the Year" in 1999. For further information on benefits and employment
opportunities, please visit our website at www.whiteoaksemi.com.

Contact:

Kim Christensen
Ph: 804 952 7307
FAX: 804 952 7902
E-mail: kim.christensen-at-infineon.com




From daemon Wed Dec 27 08:59:20 2000



From: Larry Allard :      l2a-at-ornl.gov
Date: Wed, 27 Dec 2000 09:53:58 -0500
Subject: Re: lab design book

Contents Retrieved from Microscopy Listserver Archives
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Scott:

You may be referring to Vol. 4 of Practical Methods in Electron
Microscopy, A. Glauert, ed., North Holland Pub., 1975. The book is a
monograph by Ronald H Alderson on "Design of the Electron Microscope
Laboratory". It has a lot of good information, but may be getting a
bit dated for the ultimate level of environment for today's most
sophisticated and sensitive instruments.

hope this helps...

Larry






} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

Dr. Lawrence F. Allard
Senior Research Staff Member
High Temperature Materials Laboratory
Oak Ridge National Laboratory
1 Bethel Valley Road
Bldg. 4515, MS 6064
PO Box 2008
Oak Ridge, TN 37831-6064

865-574-4981
865-576-5413 Fax
allardlfjr-at-ornl.gov



From daemon Wed Dec 27 19:19:55 2000



From: Rinaldo Pires dos Santos :      rinaldop-at-uol.com.br
Date: Wed, 27 Dec 2000 19:00:20 -0600
Subject: LR White protocol for plant tissues

Contents Retrieved from Microscopy Listserver Archives
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Hi,
I am looking for a protocol for embedding plant tissues in LR White resin. I
am working with endosperm and plant embryo of seeds of Ilex paraguariensis
(maté). The endosperm contains a large amount of proteins and lipids.
Thank's.

Dr. Rinaldo Pires dos Santos
Dept. of Botany - UFRGS
Brazil
E-mail: rinaldop-at-uol.com.br
Tel: + 55 51 4964053 (home)
+ 55 51 3167634 (UFRGS)





From daemon Thu Dec 28 01:09:34 2000



From: =?ks_c_5601-1987?B?Sm9uZG8gWXVuIMCxwbi1tQ==?= :      jdyun-at-kyungnam.ac.kr
Date: Thu, 28 Dec 2000 15:38:21 +0900
Subject: Re: Thickness Measurement

Contents Retrieved from Microscopy Listserver Archives
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Dear Sergey, Tobias and other listers:

Thank you for your kind replies.
The equipment that you mentioned seems too expensive for me to use, especially when I need just a couple of times thickness measurement for the calibration of my sputter coater.
Is there other way to measure the thickness? Tobias suggested to use ellipsometry technique because the metallic or carbon coating transmits light when it is very thin. Have any you ever tried this before?

Best wishes,

Jondo Yun
Kyungnam University, Korea
jdyun-at-hanma.kyungnam.ac.kr

====================================================================
Hello Jondo

It looks like nobody answer your question.

There are special machines called "thickness monitor" on the market. You
have to go to your preferable EM supplier and ask them about that. It's
very usual piece of equipment. It measures directly the thickness of the
deposited material on the sensor. Sensor is mounted inside the vacuum
chamber close to your sample. It is pretty sensitive (down to the 0.1
nm). The cost - couple of thousand backs. If you have further questions -
I'll be happy to help you.

Have a good holiday season.

Sergey.

Sergey Ryazantsev, Ph.D.
Electron Microscopy
Department of Biological Chemistry, School of Medicine
University of California, Los Angeles
Box 951737
Los Angeles, CA 90095-1737

(310) 825-1144 (office)
Pager: (310) 845-0248
FAX: (310) 206-5272 (departmental)
mailto:sryazant-at-ucla.edu

===============================================================
Jondo,
Keep in mind that unless you deposit a really thick coating,
carbon or metal coats do transmit light so ellipsometry could work. I
have no personal experience with this, I just wanted to make the
point. Good luck. TB


} Jondo,?------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

--
_ ____ __ ____ Tobias I. Baskin
/ \ / / \ / \ \ 109 Tucker Hall
/ / / / \ \ \ Biological Sciences
/_ / __ /__ \ \ \__ University of Missouri
/ / / \ \ \ Columbia, MO USA
/ / / \ \ \ 65211-7400
/ / ___ / \ \__/ \ ____ voice: 573-882-0173
fax: 573-882-0123





From daemon Thu Dec 28 11:09:31 2000



From: Timothy Schneider :      Timothy.Schneider-at-Mail.TJU.EDU
Date: Thu, 28 Dec 2000 11:52:29 -0500
Subject: Uranyl Acetate in Acetone

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello Out There:

I am currently involved with a cryo substitution project with human red
blood cells. I have replaced the non crystalline ice with 2% Osmium in 100%
acetone and have OK morphology, but the contrast is a bit low. I am
thinking about trying to follow the Osmium step with uranyl acetate, before
embedding in plastic. Does any one out there know if 1%uranly acetate will
be OK in 100% acetone? Thanks, Tim

Timothy G. Schneider
Director of Electron Microscopy
Department of Pathology
Room 229 Jefferson Hall
Thomas Jefferson University
1020 Locust St.
Philadelphia Pa. 19107
215-503-4798 work
610-613-8170 cellular
timothy.schneider-at-mail.tju.edu




From daemon Fri Dec 29 08:52:00 2000



From: msoulek-at-foodprorecruiters.com
Date: Fri, 29 Dec 2000 08:28:33 -0600
Subject: Available position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Email: msoulek-at-foodprorecruiters.com
Name: Michael Soulek

Available position - Requesting interested parties or help with this
assignment.


I am looking for a person to fill an available Sr. Microscopist position in
the Midwest. Salary of $60-75k and a 5% bonus potential. Desire PHD or
equivalent experience in Histo and or Micro Chemistry area of knowledge.
Selected candidate will work in a million dollar + lab in a service group
on problems that are brought to the group to solve. Solid communication
and management of priorities is strongly sought in this person.

Food or plant background is sought after experience. Company does work on
a World Wide Basis

Some of the projects are to work in coloring,marking,compound ID,looking at
the different types to identify the various types of
proteins,carbohydrates,lipids or elements of the various tissues. The last
hired person came from USDA.

Contact
Michael Soulek
FOODPRO Recruiters Inc
210-494-9272
msoulek-at-foodprorecruiters.com

---------------------------------------------------------------------------





From daemon Fri Dec 29 12:34:02 2000



From: Alexander Mironov :      mironov-at-alpha400.cmns.mnegri.it
Date: Fri, 29 Dec 2000 19:29:21 +0100 (MET)
Subject: Re: Uranyl Acetate in Acetone

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Timothy,
We also worked with acetone and I have found that if one uses 4% OsO4 in
acetone the contrast should be rather good.

Happy New Year!

Yours, Sasha

On Thu, 28 Dec 2000, Timothy Schneider wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Hello Out There:
}
} I am currently involved with a cryo substitution project with human red
} blood cells. I have replaced the non crystalline ice with 2% Osmium in 100%
} acetone and have OK morphology, but the contrast is a bit low. I am
} thinking about trying to follow the Osmium step with uranyl acetate, before
} embedding in plastic. Does any one out there know if 1%uranly acetate will
} be OK in 100% acetone? Thanks, Tim
}
} Timothy G. Schneider
} Director of Electron Microscopy
} Department of Pathology
} Room 229 Jefferson Hall
} Thomas Jefferson University
} 1020 Locust St.
} Philadelphia Pa. 19107
} 215-503-4798 work
} 610-613-8170 cellular
} timothy.schneider-at-mail.tju.edu
}
}
}




From daemon Fri Dec 29 16:00:06 2000



From: Douglas Keene :      DRK-at-shcc.org
Date: Fri, 29 Dec 2000 13:52:56 -0800 (Pacific Standard Time)
Subject: Re: Thickness Measurement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Jondo,

Can you deposit your coating onto a sample of polymerized
TEM embedding media, then cut the sample at 90 degrees on a
microtome, then measure the thickness using a TEM?

Good luck,

Doug

On Thu, 28 Dec 2000 15:38:21 +0900
=?ks_c_5601-1987?B?Sm9uZG8gWXVuIMCxwbi1tQ==?=
{jdyun-at-kyungnam.ac.kr} wrote:

}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy
} Society of America To Subscribe/Unsubscribe -- Send Email
} to ListServer-at-MSA.Microscopy.Com On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} -----------------------------------------------------------------------.
}
}
} Dear Sergey, Tobias and other listers:
}
} Thank you for your kind replies.
} The equipment that you mentioned seems too expensive for
} me to use, especially when I need just a couple of times
} thickness measurement for the calibration of my sputter
} coater. Is there other way to measure the thickness?
} Tobias suggested to use ellipsometry technique because the
} metallic or carbon coating transmits light when it is very
} thin. Have any you ever tried this before?
}
} Best wishes,
}
} Jondo Yun
} Kyungnam University, Korea
} jdyun-at-hanma.kyungnam.ac.kr
}
} ====================================================================
} Hello Jondo
}
} It looks like nobody answer your question.
}
} There are special machines called "thickness monitor" on
} the market. You have to go to your preferable EM supplier
} and ask them about that. It's very usual piece of
} equipment. It measures directly the thickness of the
} deposited material on the sensor. Sensor is mounted inside
} the vacuum chamber close to your sample. It is pretty
} sensitive (down to the 0.1 nm). The cost - couple of
} thousand backs. If you have further questions - I'll be
} happy to help you.
}
} Have a good holiday season.
}
} Sergey.
}
} Sergey Ryazantsev, Ph.D.
} Electron Microscopy
} Department of Biological Chemistry, School of Medicine
} University of California, Los Angeles
} Box 951737
} Los Angeles, CA 90095-1737
}
} (310) 825-1144 (office)
} Pager: (310) 845-0248
} FAX: (310) 206-5272 (departmental)
} mailto:sryazant-at-ucla.edu
}
} ===============================================================
} Jondo,
} Keep in mind that unless you deposit a really thick
} coating, carbon or metal coats do transmit light so
} ellipsometry could work. I have no personal experience with
} this, I just wanted to make the point. Good luck. TB
}
}
} } Jondo,?------------------------------------------------------------------------
} } The Microscopy ListServer -- Sponsor: The Microscopy
} Society of America } To Subscribe/Unsubscribe -- Send Email
} to ListServer-at-MSA.Microscopy.Com } On-Line Help
} http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------.
} }
} } } Dear Listers:
} } } Can anybody suggest how to measure thickness of the
} conductive } coating for EM specimen? If it is transparent,
} ellipsometer could be } used. Since metallic or carbon
} coating is not transparent, I could } not use that method.
} Then do I have only way to measure directly, } like
} measuring thickness on the cross-section or on the surface
} by } using EM or SPM? Is there any other way?
} } } Jondo Yun
} } jdyun-at-kyungnam.ac.kr
}
} --
} _ ____ __ ____ Tobias I. Baskin
} / \ / / \ / \ \ 109
} Tucker Hall / / / / \ \ \
} Biological Sciences /_ / __ /__ \ \ \__
} University of Missouri / / / \ \
} \ Columbia, MO USA / / /
} \ \ \ 65211-7400 / / ___ / \
} \__/ \ ____ voice: 573-882-0173 fax:
} 573-882-0123
}
}
}

----------------------
Douglas R. Keene
Associate Investigator
Shriners Hospital Research Facilities
3101 S.W. Sam Jackson Park Road
Portland, Oregon 97201
phone: 503-221-3434
FAX: 503-412-6894 (9-5 PST)
e-mail: DRK-at-shcc.org








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