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From: zaluzec-at-microscopy.com
Date: Mon, 1 Jan 2007 10:48:51 -0600
Subject: [Microscopy] Adminsitrivia: 2006 Listserver Archives on-line

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Happy New Year Colleagues

Welcome to the 14th year of the Microscopy Listserver operation.

The complete searchable Microscopy Listserver archive for 2006
(~11.8 Mb) is now on-line at:

http://www.microscopy.com

Last year we delivered nearly 4.8 Million messages to subscribers
around the world (~198.4 Gbits of traffic). I hate to say it but as
in preceeding years spam attacks continue to increase. In 2006,
the custom filters on the Listserver blocked 127,504 suspect postings for
an average of 349.3 suspect spam messages/day. Just imagine
what that would have done to your in-boxes as well as to the utility of
this server.

As you probably realize, a infinitesimal number of those suspect Emails
were actually from subscribers. The major problem continues to
be hidden attachments.

Please if your message is rejected, read the entire rejection message for
the reason and remember check the settings on your Email client program
to insure that it is set to send only plain text messages. Most Email clients
send formatted text as a hidden attachment to your mail, and this will be
stopped by the Email filters. As always, there are no exceptions allowed.
If you can't change the settings use the WWW based posting form at
http://www.microscopy.com, this will strip any attachments
from your message.

Cheers,

Nestor
Your Friendly Neighborhood SysOp

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From: microscopytoday-at-tampabay.rr.com
Date: Mon, 1 Jan 2007 11:32:58 -0600
Subject: [Microscopy] Microscopy Today January 2007 Table of Contents

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Listers,

Here is the January 2007 Microscopy Today table of contents. I will
close the subscription list for this issue on Monday, Jan. 8th, 2007.

Microscopists in North America and MSA members anywhere can subscribe
for free. Anyone else may subscribe for US$50 per year (to PARTIALLY
cover postage). All subscriptions at http://www.microscopy-today.com
Thank you.

Ron Anderson, Editor
================================

Why Penguin Beaks are Sexy!
Stephen W. Carmichael, ,Mayo Clinic

A ‘Different’ Kind of Microscopy
Fred Schamber and Kai van Beek, Aspex Corporation

Microwave Myths and Tissue Processing
Phillip McArdle, Energy Beam Sciences, East Granby, CT

Recent Developments in CrossBeam® Technology A. Thesen, H. Hoffmeister,
M. Schumann, P. Gnauck, Carl Zeiss SMT Oberkochen, Germany

Heated-Tip AFM: Applications in Nanocomposite Polymer Membranes and
Energetic Materials
Jason P. Killgore1, William King2, Kevin Kjoller3 and René M. Overney1,
1 U. of Washington, Seattle, WA, 2 U. of Illinois at Urbana-Champaign,
IL, 3 Anasys Instruments, Santa Barbara, CA

Automated S/TEM Sample Preparation for Semiconductor Process Support
Greg Cuti* and Taha Jabbar**, *Sela USA, Inc. Sunnyvale, CA, and
**Athenian Institute, Danville, CA

Serial Sectioning via Microtomy (or, How To Get Over 100 Consecutive
Serial Sections On One TEM Gird)
David Elliott, University of Arizona, Tucson, AZ

A Combined In-situ and Electron Tomography Holder for (S)TEM
C. Mitterbauer*, N.D. Browning*,** and P. V. Deshmukh***,*U. of
California, Davis, CA, **Lawrence Livermore National Lab., CA, ***E.A.
Fischione Instruments, Inc., Export, PA

Infrared Laser Confocal Microscopy: Fast, Flexible, Cost-Effective
Inspection, and Metrology Tool for Microelectronic Manufacturing
David Rideout, Olympus Micro-Imaging Orangeburg, NY

New Approaches to Managing, Marketing, and Money for Maintaining a Core
Facility (D. Sherman, Organizer)
Part Ia: Case Study: Strategic plan for an EM Facility
Elaine Humphrey

Part Ib: How to Make a Business Plan for Facility Maintenance and Growth
Donald A. Blewett, Purdue University

A Note on Storing and Testing Gold Conjugates
Jan Leunissen, Aurion, Costerweg, The Netherlands

Cross-sectional TEM Sample Preparation for Nanowires or Porous Films
Grown on a Substrate
Chengyu Song, NCEM, Lawrence Berkeley National Laboratory

New & Interesting at Cell Biology and Industry News

Netnotes
SAMPLE PREPARATION – buffers for fixation
SAMPLE PREPARATION – high pH buffer for fixation
SAMPLE PREPARATION – cells grown on collagen gels
SAMPLE PREPARATION - preservation of microbes in external mucous
SAMPLE PREPARATION – charcoal and wood
SAMPLE PREPARATION - embedding wood
SAMPLE PREPARATION - propylene oxide vs. acetone
SAMPLE PREPARATION - LR White flat embedding
SAMPLE PREPARATION - LR White contrast
SAMPLE PREPARATION – previously frozen tissues
SAMPLE PREPARATION - embedding acrylamide gel
SAMPLE PREPARATION – oil-in-water nanoemulsion
SAMPLE PREPARATION - cross section of multilayers on stainless substrate
SAMPLE PREPARATION – negative staining with ammonium molybdate
LM - dark field microscopy
LM - phase contrast vs. dark field
TEM – digital cameras
Crystallographic indices
EBSD - sharpness
EBIC vs. SE

Index of Advertisers


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From: carnahan-at-edison-labs.com
Date: Tue, 2 Jan 2007 08:28:38 -0600
Subject: [Microscopy] viaWWW: Tracor-Northern Xray detector

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both carnahan-at-edison-labs.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: carnahan-at-edison-labs.com
Name: Jim Carnahan

Organization: Edison Analytical Laboratories, Inc.

Title-Subject: [Filtered] Tracor-Northern Xray detector.

Question: The computer, including the 8" floppy drives, on our ancient Tracor-Northern (Noran) TN-5500 detector died some time ago but now we would like to rejuvenate the instrument. I recall some years ago a company was selling a PC upgrade that used the existing HV, pulse processor and other boards but with a modern PC interface and software. Does anyone know if that product still exists and know the source?

An alternative for us would be if anyone has written a Labview (National Instruments) driver for these old instruments that we could implement.

Thanks in advance,

Jim Carnahan

---------------------------------------------------------------------------

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From: kenconverse-at-qualityimages.biz
Date: Tue, 2 Jan 2007 09:41:15 -0600
Subject: [Microscopy] viaWWW: Tracor-Northern Xray detector

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Jim,
One company that several of my customers have been happy with is IXRF
http://www.ixrfsystems.com/
You can include a digital imaging option with it.
No financial interest just good experience with them.

Ken Converse
owner


QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
474 So. Bridgton Rd.
Bridgton, ME 04009
207-647-4348
Fax 207-647-2688
kenconverse-at-qualityimages.biz
qualityimages.biz



-----Original Message-----
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Sent: Tuesday, January 02, 2007 9:32 AM
To: kenconverse-at-qualityimages.biz

This Question/Comment was submitted to the Microscopy Listserver using the
WWW based Form at
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying

please copy both carnahan-at-edison-labs.com as well as the MIcroscopy
Listserver
---------------------------------------------------------------------------

Email: carnahan-at-edison-labs.com
Name: Jim Carnahan

Organization: Edison Analytical Laboratories, Inc.

Title-Subject: [Filtered] Tracor-Northern Xray detector.

Question: The computer, including the 8" floppy drives, on our ancient
Tracor-Northern (Noran) TN-5500 detector died some time ago but now we would
like to rejuvenate the instrument. I recall some years ago a company was
selling a PC upgrade that used the existing HV, pulse processor and other
boards but with a modern PC interface and software. Does anyone know if
that product still exists and know the source?

An alternative for us would be if anyone has written a Labview (National
Instruments) driver for these old instruments that we could implement.

Thanks in advance,

Jim Carnahan

---------------------------------------------------------------------------

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From: NWWhite-at-bwxt.com
Date: Tue, 2 Jan 2007 12:19:41 -0600
Subject: [Microscopy] Photoshop glitch - Followup

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Happy New Year!

Original problem: File type omitted when saving images(E.G. picture
instead of picture.tif). Also some menu windows had all the graphics,
but omitted the text describing the "radio buttons" function. No other
programs were malfunctioning. A photoshop reinstall did not fix the
issue.

The problem was finally traced to something odd in the Windows 2000 user
file. Have not figured out what or why, however. I installed an older
hard drive clone, updated the windows install and now PS works fine.

Plan to clone the now functional (was backup clone) drive onto the
problematic one. Looks like I will never find out what exactly happened
- but it works so...

Thanks for all the suggestions,

Woody White
BWXT Services



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From: pbozzano-at-cnea.gov.ar
Date: Tue, 2 Jan 2007 12:26:16 -0600
Subject: [Microscopy] EMS on line

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi listers!
I used to run the free EMS on line (http://cimesg1.epfl.ch/CIOL) to draw
SAD and Kikuchi patterns, (hkl) distances, indexing SAD patterns, etc.,
but I am having problems now. The legend "This calcul has failed for an
undefined reason" always appeared when I try to run a routine. Can
anybody help me with it?
Thanks in advanced!
Patricia



---------------------------------------------
Dra. Patricia B.Bozzano
Lab. de Microscopia Electrónica
Centro Atómico Constituyentes
Comisión Nacional de Energía Atómica
Buenos Aires, Argentina
pbozzano-at-cnea.gov.ar
Tel: 54 11 6772 7395
Fax: 54 11 6772 7362





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From: johnf-at-geology.wisc.edu
Date: Tue, 2 Jan 2007 13:20:16 -0600
Subject: [Microscopy] Re: viaWWW: Tracor-Northern Xray detector

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

}
} Email: carnahan-at-edison-labs.com
} Name: Jim Carnahan
}
} Organization: Edison Analytical Laboratories, Inc.
}
} Title-Subject: [Filtered] Tracor-Northern Xray detector.
}
} Question: The computer, including the 8" floppy drives, on our
} ancient Tracor-Northern (Noran) TN-5500 detector died some time ago
} but now we would like to rejuvenate the instrument. I recall some
} years ago a company was selling a PC upgrade that used the existing
} HV, pulse processor and other boards but with a modern PC interface
} and software. Does anyone know if that product still exists and
} know the source?
}
} An alternative for us would be if anyone has written a Labview
} (National Instruments) driver for these old instruments that we
} could implement.
}
} Thanks in advance,
}
} Jim Carnahan

Jim

One company that provides the service you mentioned is TN Analyzer,
www.tnas.net. (run by a former Tracor-Northern engineer). We upgraded
to their WinEDS system several years ago, at a very reasonable cost,
and are happy with the product.

John
--
========================================================
John Fournelle, Ph.D. office: (608) 262-7964 cell: (608) 438-7480
Cameron Electron Microprobe Lab lab: (608) 265-4798
Dept of Geology & Geophysics fax: (608) 262-0693
University of Wisconsin home: (608) 274-2245
1215 West Dayton St. email: johnf-at-geology.wisc.edu
Madison, WI 53706 amateur radio: WA3BTA
Personal http://www.geology.wisc.edu/~johnf/
Probe lab http://www.geology.wisc.edu/~johnf/sx51.html
Probe Sign Up Calender:
http://www.microscopy.wisc.edu/cgi-bin/calendar/microprobe/calendar.cgi

"The first rule of all intelligent tinkering is to save every cog and
wheel." -- Aldo Leopold

"For a successful technology, reality must take precedence over
public relations, for Nature cannot be fooled." -- Richard P.
Feynman

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From: wesaia-at-iastate.edu
Date: Tue, 2 Jan 2007 15:30:13 -0600
Subject: [Microscopy] AskAMicroscopist: solid state versus a scintilater

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We have both types of detector on one of our SEMs. While I can offer you some distinctions, others may have newer models and care to join the discussion.
 
Our SEM was originally supplied with a Robinson scintillator style detector. Its strengths were fast response for rapid scan rates up to and including TV-rate. It is good for general imaging and gives a nice topographic effect.
 
We later purchased a solid-sate detector for particular use for image analysis. Our application required lower voltage (6kV) than normal. We also required an even response across the field of view. For that, the solid-state detector was considerably better, but it has a slower response than the Robinson detector.
 
So the better detector will depend on your requirements. As it is, we run the solid state most of the time.
 
Warren Straszheim

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Below is the result of your feedback form (NJZFM-ultra-55).  It was submitted by  (wawennekes-at-woh.rr.com) from http://www.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Friday, December 29, 2006 at 20:19:28
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Email: wawennekes-at-woh.rr.com
Name: Willem Wennekes

Organization: UES

Education: Graduate College

Location: Dayton, Ohio, USA

Question: What is the difference in performance between a solid state & a scintilater (Robinson) backscatter detector?  I am very interested in a side by side comparison.

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From: sales-at-tradezone.net
Date: Tue, 2 Jan 2007 19:57:39 -0600
Subject: [Microscopy] viaWWW: Tracor-Northern X-ray detector

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Email: sales-at-tradezone.net
Name: Michael S

Organization: TradeZone.net

Title-Subject: [Filtered] Tracor-Northern X-ray detector

Question: At one time Evex (www.evex.com) of Princeton, NJ supplied x-ray microanalysis systems that interfaced to older systems like your Tracor 5500.

FYI: Though we currently don't have an inventory of tracor parts, you may want to check back with us in a few weeks to see if that changes.


Michael S.
Used Laboratory Equipment Broker
sales-at-TradeZone.net

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From: Martin.Hoppe-at-leica-microsystems.com
Date: Wed, 3 Jan 2007 08:08:33 -0600
Subject: [Microscopy] viaWWW: Re: Understanding STED

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Email: Martin.Hoppe-at-leica-microsystems.com
Name: Martin Hoppe

Organization: Leica Microsystems

Title-Subject: [Filtered] Re: Understanding STED

Question: In order to increase resolution with STED, it is not necessary to have a diffraction-unlimited detection point spread function (PSF). In STED, the diffraction-unlimited sharp excitation PSF is superimposed by the larger diffraction-limited detection PSF. In order to separate (=resolve) 2 adjacent points or molecules, they can both be located within the larger detection PSF. In STED, these points are sequentially excited due to the sharp excitation PSF in conjunction with scanning the beam across the specimen.

Regards
Martin

--------------------------------------------------------
Martin Hoppe, Ph.D.
Global Market Manager
Advanced Fluorescence Systems
Leica Microsystems CMS GmbH
Am Friedensplatz 3
D 68165 Mannheim / Germany

Phone +49 (0)621-7028-0
Cell phone: +49 (0) 172-623-0409
Fax: + 49 (0) 621-7028-1180
Email. Martin.Hoppe-at-leica-microsystems.com



-----------------------------------------------------
Email: onkel98-at-yahoo.com
Name: pipetman

Title-Subject: [Filtered] Q: understanding STED?

Question: I have a problem conceptually understanding STED.

I can see, how a diffraction-limited spot of excited fluorophores is shaped to narrow the PSF. As a result, I get something akin to a point source of emission. But as the emitted light travels back through the microscope's optics, it is subject to diffraction again (and should thus destroy the narrowed PSF).

Is there somewhere a Gaussian fit applied at the other end (similar to PALM) to trace back the original spot? Or am I missing something basic here?

Any explanation would be greatly appreciated. Thanks - p



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From: denniscward-at-earthlink.net
Date: Wed, 3 Jan 2007 14:17:27 -0600
Subject: [Microscopy] follow-up: Appeal for Spectra

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Recently I made an "appeal for spectra" to this listserve. To those who responded, we sincerely appreciate your contributions, and enthusiasm for the project.
To those who did not respond because of my use of a personal email address, I have included my "FBI domain" address in the signature below.
At the risk of redundancy I will repeat my original appeal (OK, Nestor?).
Again, thank you.

} } }
SEM/EDS has been important analytical instrumentation for the FBI for decades. We have recently expanded its utility in investigative roles, however, and therefore would like to ask the community for assistance.
Whereas most SEM/EDS users need answers provided by quantitation and structural characterization, forensic inquiries generally necessitate "identifications"of questioned materials. This is generally possible only if the analyst is able to search his analytical results (spectra, primarily) against data from reference materials, as is the case with most other mature spectroscopies. We therefore have developed tools to archive and query the spectra and associated metadata of reference materials, and have produced a data set of thousands of materials in a variety of materials categories. Spectral and data queries against this database are consistently providing significant investigative direction. Of course quantitative analysis and other analytical techniques are additionally used where appropriate.
Because the FBI has limited analytical capability and limited access to specific materials, I am appealing to you for either reference spectra that can be uploaded directly into our database, or materials that we can borrow and analyze at our facility (on a very limited, very specific basis). Literally all materials are viable candidates for inclusion, from commercial products to biologicals. Spectra must be exportable in EMSA format.
Any data contributed will be restricted to FBI use only. If you would be willing to consider participating in this project, please contact me directly for details.
We sincerely appreciate your consideration of this appeal!

Dennis Ward, FBI



Dennis Ward
FBI Laboratory
Chemistry Unit
2501 Investigation Parkway
Quantico, VA 22135
v 703.632.7424

denniscward-at-earthlink.net
Dennis.Ward-at-ic.fbi.gov


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From: mrsean-at-u.washington.edu
Date: Wed, 3 Jan 2007 19:18:51 -0600
Subject: [Microscopy] viaWWW: Can I process frozen tissue for TEM?

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Email: mrsean-at-u.washington.edu
Name: Sean Kelly

Organization: University of Washington

Title-Subject: [Filtered] Can I process frozen tissue for TEM?

Question: I have some tissue that is embedded in OCT and stored at
-70C and want to know if it can be processed for TEM.

Any experience with this or relevent publications would be greatly appreciated.

Thanks,
Sean

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From: stuart-at-foto45.co.uk
Date: Wed, 3 Jan 2007 19:20:24 -0600
Subject: [Microscopy] viaWWW: Photography Masters Project

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Email: stuart-at-foto45.co.uk
Name: Stuart Handley

Title-Subject: [Filtered] Photography

Question: Hi,the reason for ask a massive favour is, I am starting a masters degree in photography and related media in a few days time and need to put my proposal in for my research project. I am going to propose images and related images on the subject of mines and minerals. This will involve photography underground and of samples that I collect, my hope is that I can find an electron microscopists who can help me to get down to the micro level of the minerals. The photography has to be different or done in a way that has not been done before so by combining the conventional and the electro scanning microscope images plus studio images of minerals I will be able to achive my set goal. Can any one out there help in any way or put me on to some one who maybe able to. Finding access to such equipment is going to be hard, any help most appreciated, I'm doing the MA part time over three years.
Stuart Handley

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From: jbs-at-temple.edu
Date: Wed, 3 Jan 2007 20:43:20 -0600
Subject: [Microscopy] AO Ultracut

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Greetings and Happy New Year.

Does anyone know the current price of the latest version of the old
AO Ultracut microtome?

Joel



Joel B. Sheffield, Ph.D
Department of Biology
Temple University
Philadelphia, PA 19122
Voice: 215 204 8839
e-mail: jbs-at-temple.edu


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From: lcgould-at-med.cornell.edu
Date: Thu, 4 Jan 2007 08:06:52 -0600
Subject: [Microscopy] Re: Can I process frozen tissue for TEM?

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Hi Sean,
Can you process it? Yes.
Should you process it? Maybe.
Will you get good ultrastrucure? No.

A lot will depend on how the tissue was treated before it was frozen:
was it flash frozen without any fixation or cryo-protection? It will
have a lot of freezing artefacts at the EM level...ice crystal damage
to membranes, etc.
If it was lightly fixed (eg:paraformaldehyde), it might fair a bit better.
If it was fixed AND cryo-protected (eg: treated with sucrose), you
might get some decent structure.

You'll have to cut away as much of the OCT as you can and then thaw
the tissue. I would advise thawing it in a buffered glut. solution
so that it fixes as it thaws. Then process for TEM as usual and keep
you fingers crossed.
Over the years, I've had to process tissues that were not originally
intended for EM and I've been surprised by what may be rescued.
Good luck,
Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy & Histology Core Facility
Manager, Optical Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175
http://www.cornellcelldevbiology.org
http://www.cornellbiochem.org

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From: bfoster-at-mme1.com
Date: Thu, 4 Jan 2007 11:33:04 -0600
Subject: [Microscopy] Re: viaWWW: Photography Masters Project

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi, Stuart

If you are looking for art, I'd really suggest that you consider polarized light (an optical microscopy technique). The sample prep is about the same and the results are incredibly beautiful.

I have a colleague in the UK who can probably help. Contact me off-line for further info.

Good hunting!
Barbara Foster

Microscopy/Microscopy Education
313 S Jupiter Rd, Suite 100
Allen, TX 75002
P: 972-954-8011
W: www.MicroscopyEducation.com


MME is now scheduling customized, on-site courses through April. Call us today for details.

P. S.
Need a good general reference or light microscopy text for the Spring semester? Call us today to learn more about "Optimizing LIght Microscopy". Copies still available through MME... even for class-room lots ... and we give quantity discounts. Call Ken Piel at (972)954-8011.


At 11:26 AM 1/4/2007, stuart-at-foto45.co.uk wrote:



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From: bob-at-rockisland.com
Date: Thu, 4 Jan 2007 15:16:17 -0600
Subject: [Microscopy] viaWWW: Photography Masters Project

Contents Retrieved from Microscopy Listserver Archives
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Hi Stuart,

There is a neat book: "Photography Through The Microscope" By J. G.
Delly and Eastman Kodak. It has many examples of photos taken through light
microscopes along with easy to follow 'how to...' instructions. My copy was
published in 1988. I don't know if the book is still in print, but your
University library can most likely has it or find a copy for you.

Take care, Bob Carter
2000 Bayshore Road
Lopez Island, WA 98261-8595

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From: steve.klingaman-at-normandale.edu
Date: Thu, 4 Jan 2007 17:55:03 -0600
Subject: [Microscopy] AskAMicroscopist: entry-level SEM for teaching

Contents Retrieved from Microscopy Listserver Archives
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This Question was submitted to Ask-A-Microscopist by (steve.klingaman-at-normandale.edu)
from http://www.msa.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Thursday, January 4, 2007 at 14:07:06
Remember to consider the Grade/Age of the student when considering the Question
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Email: steve.klingaman-at-normandale.edu
Name: Steve Klingaman

Organization: Normandale Community College

Education: Undergraduate College

Location: Bloomington, MN USA

Title: SEM

Question: Can you make a recommendation as to an entry-level SEM for teaching purposes to be used in a life sciences lab? Would a "table top" model suffice?

Price range might be the $50 - $100K range

Thank you,


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From: swatkins-at-pitt.edu
Date: Thu, 4 Jan 2007 17:55:41 -0600
Subject: [Microscopy] viaWWW: cryo-ultramicrotome wanted

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Email: swatkins-at-pitt.edu
Name: simon watkins

Organization: University of Pittsburgh

Title-Subject: [Filtered] cryo-ultramicrotome wanted

Question: Hi folks, I am trying to buy a used Reichert/Leica Cryo-ultramicrotome. I really only need the cryokit if you have one lying around or in storage. Both of our units have failed and we cant get the parts to fix them!!!! Built in obsolescence I guess. If you have a system, or know someone who would like to sell one to my group, please contact me offline
Simon

Simon C. Watkins Ph.D. FRC Path.
Professor, Cell Biology and Physiology and Immunology
Director Graduate Program in Cell Biology and Physiology
Vice Chair, Cell Biology and Physiology
Director Center for Biologic Imaging
BSTS 225
University of Pittsburgh
3500 Terrace St.
Pittsburgh PA 15261
Tel:412-648-3051
Fax:412-648-2797
URL: http://www.cbi.pitt.edu


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From: jzheng-at-uci.edu
Date: Thu, 4 Jan 2007 19:39:20 -0600
Subject: [Microscopy] question: value of CM20 TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Question: Hi, Folks,
I have a Philips CM20 traditional TEM with an EDS system. It is about 15
years old, but has a good working condition. Could you please tell me
how much it is worth now?
Thanks, Jian-Guo

------------------------------------------------------------------
Jian-Guo Zheng, PhD
Director, Nanomaterials Characterization and Fabrication Facility (NCF2)
University of California, Irvine
3415 Calit2 Building
Irvine, CA 92697-2800
phone: 949-824-0441 (office), 949-468-9980 (cell)
fax: 949-824-8197
email: jzheng-at-calit2.uci.edu
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From: nizets2-at-yahoo.com
Date: Fri, 5 Jan 2007 04:04:12 -0600
Subject: [Microscopy] low vac SEM questions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi!

Back again with my basic questions about SEM ;-)
I took pictures using both low vac mode (1 Pa) and
high vac mode (8x10^-3 Pa) at magnifications from 3kX
up to 25kX with my tescan SEM (at 10kV with quartz). I
can't see the slightest improvement in image quality!
The only difference is a slight increase in contrast
at higher vacuum (which is not necessarily better
because low-contrasted parts are not visible).

Is it normal? What would be the use of high vacuum? Is
it useful for EDX analysis but not normal scanning?

Stephane


__________________________________________________
Do You Yahoo!?
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From: W.Muss-at-salk.at
Date: Fri, 5 Jan 2007 04:13:00 -0600
Subject: [Microscopy] STAINING: Zeil Neelson, Zeil-Neilson,Zeil Neelsen, Zeil Neilsen, Ziehl-Nielson: which one is correct

Contents Retrieved from Microscopy Listserver Archives
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To all:
} Happy, Healthy and Successful New Year"

Conc:
} Zeil Neelson {, } Zeil-Neilson {, } Zeil Neelsen {, } Zeil-Neilsen {,
} Ziehl-Nielson {:
which "name"/"term" one is correct


Dear all,
I would like to ask a question which came into minds when ToC-reading the
title of apreviousely published article on:
} Zeil Neelson [sic!]and Wade-Fite stains to demonstrate medlar bodies of
chromoblastomycosis {...

Can anybody of the group could tell or inform me wether a
} Zeil-Neelson {(SIC!) stain (for TB and mycobacterium, acid fast bacilli)
really does exist or is an other, classical, histochemical, specific stain
- I always thought to correctly be called "Ziehl-Nielson"....

When googleing shortly for } Zeil-Neelson { I found out that there exist
some other "terms" sounding very similar....see above....

Have I missed something??

Any information gladly is appreciated,

regards and THANK YOU,


Wolfgang Muss
Salzburg-AUSTRIA


OR Dr. Wolfgang Muss
Head of EM-Lab
{=with pride: 25 years in operation by 2nd of Feb.2006=}
Institute of Pathology, SALK
(Salzburger Landeskliniken gemeinnuetzige GesmbH)
(privatized General Hospital Fed. State of Salzburg)
Muellner Hauptstrasse 48
A-5020 SALZBURG AUSTRIA/EUROPE

and/or/alternatively (same Lab, same address)

Paracelsus Medical Private University (PMU)
Institute of Pathology
Electron Microscopy Lab
Muellner Hauptstrasse 48
A-5020 SALZBURG, Austria/Europe
Phone work: +43+662+4482+4720
Mobile phone work:+43+662+4482-57704
Fax-No. at work: ++43+662+4482-882 ext (please, only by indicating: "c/o
W.Muss")
E-Mail work: W.Muss-at-SALK.at

Mobile-phone private: ++43+676+5 369-456
E-Mail private: wij.Muss-at-aon.at


Ankuendigung namens der (Information on behalf of)
Society for Cutaneous Ultrastructure Research (SCUR)
PLEASE VISIT THE UPDATED WEBSITE of SCUR at
} http://www.scur.org {
-------------------------------------------------------------------------
post-congress information:
33rd Annual Meeting of the SCUR, 8th-10th June, 2006, WARSZAW, Poland
For information visit the WEBSITE, http://www.scur.org/
Click: Meetings ==} Previous Meetings ==} 33rd. Ann. Meeting
or, directly:
http://orgs.dermis.net/content/e04scur/e03meetings/e775/e896/index_ger.html


Forthcoming Meetings:
34th Annual Meeting of the SCUR, June 14-16, 2007,PRAGUE
Czech Republic
Local Host: Prof. Dr. Peter ARENBERGER
e-mail: pa-at-verum.cz

correct dates of meeting to be updated ASAP
35th Annual Meeting of the SCUR, ?11th -12th? May, 2008, OTHSU near KYOTO,
Japan
Joint Meeting with the
JSUCB, the Japanese Society for Ultrastructural Cutaneous Biology



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23, 40 -- Subject: [Microscopy] STAINING: Zeil Neelson, Zeil-Neilson,Zeil Neelsen, Zeil Neilsen, Ziehl-Nielson: which one is correct
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From: michael-at-shaffer.net
Date: Fri, 5 Jan 2007 07:06:48 -0600
Subject: [Microscopy] RE: low vac SEM questions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Stephane writes ...

} Back again with my basic questions about SEM ;-) I took
} pictures using both low vac mode (1 Pa) and high vac mode
} (8x10^-3 Pa) at magnifications from 3kX up to 25kX with my
} tescan SEM (at 10kV with quartz). I can't see the slightest
} improvement in image quality!
} The only difference is a slight increase in contrast at
} higher vacuum (which is not necessarily better because
} low-contrasted parts are not visible).

You do not give any indication of the beam current you are using (possibly
the 'spot size' adjustment for your SEM). I suspect you will not realize
the real advantages of employing high vacuum unless the beam scuurent (and
spot size) is small. Better vacuum will also provide better contrast ...
But exactly what you see is specimen and preparation dependent. I suspect
the samples are coated ... But with ??

cheerios :o)
michael shaffer

SEM/MLA Research Coordinator
{http://www.mun.ca/creait/maf/}
Inco Innovation Centre
Memorial University
St. John's, Newfoundland



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From: pburcke-at-utnet.utoledo.edu
Date: Fri, 5 Jan 2007 07:48:29 -0600
Subject: [Microscopy] viaWWW: EDS: Link exL had drive

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Email: pburcke-at-utnet.utoledo.edu
Name: Pannee Burckel

Organization: Chemistry, University of Toledo

Title-Subject: [Filtered] EDS: Link exL had drive

Question:
Dear Listers: The Link exL hard drive crashed a month ago. We need an inexpensive temporarily solution. If anyone have a it somewhere in the lab, please let me know. It is a 40S 40MB Quantum Prodrive. I could purchase an HD online but I am not sure if it can be installed with Link software. Thank you.

Pannee

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From: Anjeanette.Ormonde-at-unilever.com
Date: Fri, 5 Jan 2007 07:58:28 -0600
Subject: [Microscopy] Supplies for film processing to give away

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


In cleaning out the lab I came across a drawer full of small cylinders
that have "Polaroid Print Coater" embossed on one side and "For Black &
White Pictures Only". Inside is a fluid drenched material with a handy
little holder along one side. I've never used them and we no longer
have need to process film in the dark room but I believe the material is
used to add a protective coating to the processed photographs. Is there
anyone that would be interested in having some or all of these
materials? In addition we have some powdered Kodak Developer D-19 and
while I can't find an expiration date I'm sure it is several years old
(I've been here over 5 years and it was purchased before my arrival.)
There is also some EM film I'm sure has expired as well as some polaroid
film and even a role of film called "Kodachrome 64". I also came across
some liquid activator and fixer that was never opened. If you are
interested in any or all of these items please contact me directly for
more details.

Thanks!
Angie


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From: Sven.Terclavers-at-med.kuleuven.be
Date: Fri, 5 Jan 2007 08:10:56 -0600
Subject: [Microscopy] Stereology analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

First of all, my best wishes for 2007 - May the Light shine through your
Microscope!

As often in this lab however, the beginning of a new year brings new
challenges. We are looking for software to perform stereology analysis
(Cavalieri, Fractionator, ...). At the moment we are using already one
packge from Zeiss, incorporated into their KS300 software, however, we would
like to expand the analysis to more pc's. Therefore the following question:
is someone out there using the stereology modules for Image J? And if so,
is there a manual available or could you pass some tips?
Many thanks in advance,

Sven

°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°
Sven Terclavers
Bio-Imaging Facility
Center for Transgene Technology & Gene Therapy
Campus Gasthuisberg O&N3
Herestraat 49
3000 Leuven
Belgium
Tel: +32.(0)16.34.71.97
Fax: +32.(0)16.34.59.90
Email: Sven.Terclavers-at-med.kuleuven.be
Web: www.vib.be - www.kuleuven.be/mcm
°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°°


Disclaimer: http://www.kuleuven.be/cwis/email_disclaimer.htm



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From: renaudgeological-at-execulink.com
Date: Fri, 5 Jan 2007 14:47:06 -0600
Subject: [Microscopy] viaWWW: WIN 30 PGH Board

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi

One of the problems with assessing SEM performance is often the specimen you
select. I travel around the world looking at customers instruments training
the people to obtain more from their instrument. In order to judge the
performance of the instruments I spent a number of months experimenting with
test specimens until a came up with one where the image always changed in
some way when I changed parameters.

I remember a ListServer posting some time ago when a scientist commented
that the new FEG instruments are amazing as they are just as good (with
their specimen) at 1kV as 30kV! In my mind the test specimen was not
testing and I feel this could be the reason that you do not see changes when
you vary the parameters in your instrument.

I poor quality vacuum is great to reduce charge. But a poor vacuum causes
beam spread, a problem that will increase the spot size and, if sufficient
performance is demanded, ultimately degrade the image quality.

Steve Chapman
Senior Consultant Protrain
For electron microscopy consultancy and training world wide
Tel +44 1280 816512 Fax +44 1280 814007
Mobile +44 7711 606967 Web www.emcourses.com

----- Original Message -----
X-from: {nizets2-at-yahoo.com}
To: {protrain-at-emcourses.com}
Sent: Friday, January 05, 2007 10:05 AM

Dear Stephane,
There are several fundamental differences between high vac and low vac that
make each unique in application.
1. low vac can only use BSE or special low vac SE-type detectors that make
traditional, high-resolution SE images impossible. Low vac is usually 10 to
200 Pa. Usually these detectors require higher beam voltage (10 to 20 kV)
and beam current than high-vac SE imaging.
2. High vac requires that a sample be conductive. Try your experiment with a
freshly-plucked flower, looking at the stamen and pollen grains and you will
see that it is impossible in high vac mode but quite possible in low vac.
You may have to increase the gas pressure to 20 to 100 Pa to eliminate
charging. If you need to do high resolution imaging of the small features on
a sample surface, only high vac will allow you to use {5 kV, small aperture,
short working distance and low beam current to get detailed SE images of the
sample.
3. The scattering of the electron beam in low vac mode gives EDS
contributions from areas away from where you may want to analyse. It is nice
to be able to do EDS on anything without coating, but if you need to analyse
one small spot without contribution from neighbouring areas, you need high
vac.
Both modes have their place and strengths and it is nice to have the choice.
Regards,
Mary Mager
Electron Microscopist
Materials Eng. UBC
#419 - 6350 Stores Rd.
Vancouver, B.C. V6T 1Z4
CANADA
Phone: 604-822-5648
Fax: 604-822-3619
email: mager-at-interchange.ubc.ca

-----Original Message-----
X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com]
Sent: Friday, January 05, 2007 2:14 AM
To: mager-at-interchange.ubc.ca

Hi!

Back again with my basic questions about SEM ;-) I took pictures using both
low vac mode (1 Pa) and high vac mode (8x10^-3 Pa) at magnifications from
3kX up to 25kX with my tescan SEM (at 10kV with quartz). I can't see the
slightest improvement in image quality!
The only difference is a slight increase in contrast at higher vacuum (which
is not necessarily better because low-contrasted parts are not visible).

Is it normal? What would be the use of high vacuum? Is it useful for EDX
analysis but not normal scanning?

Stephane


__________________________________________________
Do You Yahoo!?
Tired of spam? Yahoo! Mail has the best spam protection around
http://mail.yahoo.com

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Email: renaudgeological-at-execulink.com
Name: Jim Renaud

Organization: Renaud Geological Consulting Ltd.

Title-Subject: [Filtered] WIN 30 PGH Board

Question: I am looking for a WIN 30 PGH board for the digital imaging
system on my electron microprobe. I have checked with the
manufacturer and this is a discontinued product. If you have one of
these boards and would like to sell it or know here I may be able to
find one, I would appreciate any information you could share.

Best Regards,

Jim Renaud.

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From: eoptics-at-mcmaster.ca
Date: Fri, 5 Jan 2007 16:53:37 -0600
Subject: [Microscopy] viaWWW: Conical Dark Field Imaging

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Email: eoptics-at-mcmaster.ca
Name: Fred Pearson

Organization: McMaster Unviersity/Canadian Center for Electron Microscopy

Title-Subject: [Filtered] Conical Dark Field Imaging

Question: Any there any descriptive articles available for Conical
Dark Field imaging and its advantages?



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From: Lori_mahaffey-at-yahoo.com
Date: Mon, 8 Jan 2007 08:05:07 -0600
Subject: [Microscopy] [Filtered] AskAMicroscopist: dust mites

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This Question was submitted to Ask-A-Microscopist by (Lori_mahaffey-at-yahoo.com)
from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Sunday, January 7, 2007 at 17:14:47
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Email: Lori_mahaffey-at-yahoo.com
Name: Keaton Mahaffey

Organization: Noddin Elementary

Education: K-8 Grade Grammar School

Location: San Jose Ca

Title: Dust Mites

Question: i have tried to look at an old pillow under my microscope hoping to see dust mites but i did not.
Is there something i can do to see them?

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From: cammer-at-aecom.yu.edu
Date: Mon, 8 Jan 2007 09:04:27 -0600
Subject: [Microscopy] Nikon to Olympus objective converter

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

We have a brass coupler with threads to put a Nikon objective on an
Olympus nosepiece. Don't know where it came from, but it looks homemade.

We need a few more.

Before asking our machine shop to duplicate it, we were wondering if
anybody knows whether we can purchase them.

Thanks.
____________________________________________________________________________
Michael Cammer Analytical Imaging Facility Albert Einstein Coll. of Med.
URL: http://www.aecom.yu.edu/aif/


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From: keith.morris-at-ucl.ac.uk
Date: Mon, 8 Jan 2007 09:20:50 -0600
Subject: [Microscopy] [Filtered] AskAMicroscopist: dust mites

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html



Dear Keaton,

You should be able to see them fairly easily at 10-40x, if they are there,
as they are often over 200um long - you need a decent microscope though. You
can chemically test for their presence using a chemical card. They need 70%
humidity and food to thrive - so an old unused airing cupboard pillow might
not provide any living specimens - perhaps try a mattress that's in use.
Other than allergenic dust problems with their faeces & dead mites (which
can be severe) they are harmless.

A 4 to 120x stereo microscope should also work well, although they are a
little translucent, and they are odd looking beasties. Rather than repeat
other on-line info, have a look at:

http://ohioline.osu.edu/hyg-fact/2000/2157.html

http://www.ehso.com/ehshome/dustmites.php

http://www.dustless.com/alpha/info/mites.html

http://hgic.clemson.edu/factsheets/HGIC2551.htm

http://www.funsci.com/fun3_en/dust/dust.htm

http://www.madsci.org/posts/archives/aug98/900036716.Gb.r.html

http://www.badspiderbites.com/dust-mites.php

There an optical microscope image at

http://strano16.interfree.it/micro03.htm

Keith

_________________________

Dr Keith J Morris
Imaging Facility Manager
Institute of Ophthalmology
University College London
11-43 Bath Street
London EC1V 9EL

email: keith.Morris-at-ucl.ac.uk


-----Original Message-----
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Sent: 08 January 2007 14:12
To: keith.morris-at-ucl.ac.uk

This Question was submitted to Ask-A-Microscopist by
(Lori_mahaffey-at-yahoo.com)
from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on
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Remember to consider the Grade/Age of the student when considering the
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Email: Lori_mahaffey-at-yahoo.com
Name: Keaton Mahaffey

Organization: Noddin Elementary

Education: K-8 Grade Grammar School

Location: San Jose Ca

Title: Dust Mites

Question: i have tried to look at an old pillow under my microscope hoping
to see dust mites but i did not.
Is there something i can do to see them?

---------------------------------------------------------------------------

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From: mcauliff-at-umdnj.edu
Date: Mon, 8 Jan 2007 09:35:21 -0600
Subject: [Microscopy] decalcification of Zebrafish

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Alan,

We also decomissioned our darkroom several years ago, since we had only
one remaining client that wanted prints, and she only wanted one or two
8x10's per year. Our Durst Labrator (sp?) went to another building and
is still in use, I'm told.

I purchased an inkjet, "photo-quality" printer, in case anyone really
needed prints, but it was never used and the ink cartridges eventually
went bad.

I also paid my darkroom dues over many years. I think every piece of
work clothing I owned had developer stains and I always squinted in
normal light. I will always have a fond place in my heart for
silver-based photography, and may even go back to it someday for the
artsy-fartsy part of my life, but I will not shed a tear for it in
research work. We will soon replace our old TEM with a film- and
digital-capable new one, but I expect that we will go from thousands of
sheets of 4489 per year to tens----if that.

Cheers,
Randy

Randy Tindall
Senior EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W125 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu
On-line calendar:
http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=
Week&NavType=Both&Type=TimePlan


-----Original Message-----
X-from: Debby Sherman [mailto:dsherman-at-purdue.edu]
Sent: Thursday, January 04, 2007 4:13 PM
To: Alan Nicholls
Cc: Christopher J. Gilpin; Angela Klaus; Anlee Krupp; Ann Lehman;
Anne-Marie Girard; Annette Andrews; Anthony McCormick; Bobbie Schneider;
Brigitte Shaw; Bryony James; Chere Petty; Clive Wells; David Dorward;
David Hall; Dee Breger; Donald Gantz; Douglas Cromey; Edward Seijo;
Elaine Humphrey; Elison Blancaflor; Evelyn York; Miller, F. Scott; Frank
Macaluso; Gary Chandler; Geoffrey Williams; Halina Witkiewicz; Hendrik
Colijn; Jacob Mey; Jaime Dant; Janice Pennington; Jeffrey Hanson; Jim
Conner; John Bozzola; John Curulli; John Gardner; Jon Mulholland;
Jonathan Krupp; Joseph Kulik; Judith Drazba; Julie Getz; Karen Kelley;
Karen Stevenson; Karl Wendt; Laura Raymond; Leona Cohen-Gould; Longzhou
Ma; Louis Kerr; Luisa Dempere; Margaret Bisher; Margaret Sherwood;
Marilyn Dunlap; Mark Walters; Melanie Barfels; Michael Goheen; Michelle
Ocana; Missy Hazen; Mitchell McCartney; Neelima Shah; Norman Olson;
Patricia Connelly; Patricia Jansma; Paula Allan-Wojtas; Philip Oshel;
Phillip Russell; Rakesh Bhatnagar; Rebecca Stearns; Richard Gursky;
Robin Griffin; Rogelio Pescador; Sardiaa Plaud; Steven Barlow; Stuart
McKernan; Thomas Freeman; Thomas Murray; Timothy Maugel; Tina Carvalho;
Tsuey-Chen Long; Wendy Salmon; William L'Amoreaux; William Russin; Zsolt
Lazar; Anita K. McCauley PhD; Chunfei Li Ph.D.; Earnest W. Truby Ph. D.;
Edwina W. Westbrook (Winnie); Gary M. Brown; James Hayden (Jamie);
Kenneth L. Tiekotter; Leslie M. Eibest; Mayandi Sivaguru Ph.D. (Shiv);
Qian-Chun Yu MB Ph.D.; R. Holland Cheng; S. Kelly Sears Ph.D. B.F.A.;
Sousan Abolhassani Ph.D.; Warren Straszheim Ph.D.; Barbara maloney; Ben
Fowler; Beverly Giammara; Bill Tivol; Caroline Miller; Chaoying Ni PhD;
Christian Mellen; Christine Davis; Donald Robertson; Eduardo
Rosa-Molinar; Fred Monson PhD; Greg Ning; Hilary Holloway; Jacqueline
Mills; Jan Redick; Jeff Braswell; Jeff Horn; Judy Murphy; Kent McDonald;
Kim Christensen; Lois Anderson; Ross Jr, Louis M.; Marc Pypaert; Marilee
Sellers; Michael Dunlap; Mike Gemble; Randy Nessler; Tindall, Randy D.;
Reinhard Rachel; Richard Schalek; Ron Anderson; Rosemary White; Sara
Miller; Steve Chapman; Phillips, Thomas E.

Dear colleeagues:

Anyone have a protocol for the decalcification of adult zebrafish?

Geoff

--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************



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From: jureller-at-uchicago.edu
Date: Mon, 8 Jan 2007 11:17:03 -0600
Subject: [Microscopy] Re: Nikon to Olympus objective converter

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Alan, et al.,

Having been trained in classic darkroom B&W procedures back in late
70's/early 80's (TEM 4x5, 35mm (Plus-X, Tri-X), -- and Polaroid Type
55), we decommissioned our darkroom ~8 years ago, having moved to
digital TEM and LM imaging two years prior to that. We stocked color
film (Kodachrome, Ektachrome, Kodacolor) but that was always sent out
for processing.

Basic photographic theory and practice still applies. The only thing
that has changed is the workflow, with more emphasis at the point of
capture, rather than after-the-fact processing/printing. In fact, we
perform very little printing, as 99% of needs are in digital format. Any
prints are done with a high end Fuji Pictrography printer (silver halide
based digital print system that rivals a classical photograph!)

Also, as long as you understand the technology, an inkjet can
equal/exceed a photograph. You must carefully choose the printer &
corresponding paper, and ink media. For B&W photography, one should
examine quad tone inks (multi-tone black inks).

With digital, there is a learning curve to understand concepts of camera
sensors (what the camera is actually recording), image pixels,
resolution, how to match the proper amount of digital information for a
particular output, and file storage formats (TIF, JPG, etc) and
advantages/disadvantages of each. Everything you ever did in the
darkroom is right there in your image editor (like the consumer
Photoshop Elements or even professional strength Photoshop CS).

Best regards,

Walter F. Bobrowski
Sr. Scientist
Pfizer Global R&D
2800 Plymouth Rd.
Ann Arbor, MI 48105

TEL: 734-622-7814
FAX: 734-622-5718

"The ultimate human freedom is the ability to choose one's attitude in a
given set of circumstances." -Viktor Frankl





-----Original Message-----
X-from: TindallR-at-missouri.edu [mailto:TindallR-at-missouri.edu]
Sent: Monday, January 08, 2007 10:33 AM
To: Bobrowski, Walter

Alan,

We also decomissioned our darkroom several years ago, since we had only
one remaining client that wanted prints, and she only wanted one or two
8x10's per year. Our Durst Labrator (sp?) went to another building and
is still in use, I'm told.

I purchased an inkjet, "photo-quality" printer, in case anyone really
needed prints, but it was never used and the ink cartridges eventually
went bad.

I also paid my darkroom dues over many years. I think every piece of
work clothing I owned had developer stains and I always squinted in
normal light. I will always have a fond place in my heart for
silver-based photography, and may even go back to it someday for the
artsy-fartsy part of my life, but I will not shed a tear for it in
research work. We will soon replace our old TEM with a film- and
digital-capable new one, but I expect that we will go from thousands of
sheets of 4489 per year to tens----if that.

Cheers,
Randy

Randy Tindall
Senior EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W125 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu
On-line calendar:
http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=
Week&NavType=Both&Type=TimePlan



Hi,

We had to make a few of these up in our in-shouse shop too. However, Thorlabs
now sells RMS to M25 (and reverse) commercially for cheap

http://www.thorlabs.com/newgrouppage9.cfm?objectGroup_ID=1747

regards
justin

cammer-at-aecom.yu.edu wrote:
} ----------------------------------------------------------------------------
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} ----------------------------------------------------------------------------
}
} We have a brass coupler with threads to put a Nikon objective on an
} Olympus nosepiece. Don't know where it came from, but it looks homemade.
}
} We need a few more.
}
} Before asking our machine shop to duplicate it, we were wondering if
} anybody knows whether we can purchase them.
}
} Thanks.
} ____________________________________________________________________________
} Michael Cammer Analytical Imaging Facility Albert Einstein Coll. of Med.
} URL: http://www.aecom.yu.edu/aif/
}
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--
---------------------------------------------------------------------------
Justin E. Jureller, Ph.D.
Technical Director, IBD NanoBiology Facility, The University of Chicago
Gordon Center for Integrative Science, 929 E 57th Street, Chicago, IL 60637
office: GCIS ESB06B lab: GCIS ESB18
phone: (773) 834-3864 fax: (773) 834-1917
email: jureller-at-uchicago.edu web: http://nanobio.uchicago.edu/
---------------------------------------------------------------------------

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From: cammer-at-aecom.yu.edu
Date: Mon, 8 Jan 2007 15:14:55 -0600
Subject: [Microscopy] Olympus IX70 technical question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

try B&H Photo-Video
http://www.bhphotovideo.com/bnh/controller/home?O=NavBar&A=FetchChildren&Q=&ci=9824 ,
check "Lens to Body Adapters" and "Lens Adapters and Mounts" under "General
Lens Accessories".

Even if they don't have Olympus body to Nikon lens adapter, you may combine
necessary parts from 2 adapters, these are cheap.

Vitaly Feingold
SIA
2773 Heath Line
Duluth GA 30096
Ph. 770-232-7785
Fax 770-232-1791
www.sia-cam.com
----- Original Message -----
X-from: {cammer-at-aecom.yu.edu}
To: {vitalylazar-at-att.net}
Sent: Monday, January 08, 2007 10:05 AM

never mind, I miss-understood the posting.

Vitaly Feingold
SIA
2773 Heath Line
Duluth GA 30096
Ph. 770-232-7785
Fax 770-232-1791
www.sia-cam.com
----- Original Message -----
X-from: {vitalylazar-at-att.net}
To: {vitalylazar-at-att.net}
Sent: Monday, January 08, 2007 12:23 PM

We have an Olympus IX 70 that is about six years old that we want to
rebuild for a custom laser application.

There are two optics in the light path we need to identify. We would
greatly appreciate if anybody knows the answer to please let us
know. (The alternative it to disassemble the microscope completely
which we'd rather not do.)

The light path from the sample to the detector through the side port
is as follows:

Objective lens to window to lens to prism to window. We need to
identify the lens (B) and the prism (C). B and C are diagrammed at
http://cammer.net/temp/IX70question/

Is the lens what Olympus calls the "tube lens"?
What focal length is the lens?
How far into the UV and into the IR does the lens pass } 70%?

Similarly, for the prism, how far into the UV and into the IR does
the glass pass } 70%?

Thanks!

-Michael
____________________________________________________________________________
Michael Cammer Analytical Imaging Facility Albert Einstein Coll. of Med.
URL: http://www.aecom.yu.edu/aif/


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From: jmkrupp-at-ucsc.edu
Date: Mon, 8 Jan 2007 16:12:04 -0600
Subject: [Microscopy] digital camera withdrawals

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Greetings for the New Year:

Have I become a wimp? I cannot face the new year without my TEM digital camera.

Here is the story:

About 4 years ago a PI on campus bought a digital camera for our
central campus lab's TEM. She said anyone could use it, no problem.
She got a lot of use from it and so did everyone else.

Now she is leaving and wants to take the camera with her, leaving me
without one. None of the users who got used to the camera want to go
to film, I don't miss the chemical mixing, disposal, and darkroom
time. Users really got used to taking their pictures with them at the
end of a session on a memory stick instead of waiting half a day to
see if anything turned out and then having to figure out a filing
system for all their negatives.

What would you do if someone pulled the rug, er, uh, camera, out from
under you? I need to convince our Dean that it is really the only way
to go now a days. Is that true or have I become too lazy?

There is a chance that we might be able to cut a deal with the owner
of the camera if we offer her a fair price for it. It is a Gatan 1K x
1K Bioscan mounted at the 35 mm port. Any guesses on current value? I
know it is kind of old and might be obsolete in some circles, but
it's the only one I have and I would like to keep it until we can
afford something newer. What would be a fair offer to the owner?

Jon
--


Jonathan Krupp
Microscopy & Imaging Lab
C230 Earth & Marine Science
University of California
Santa Cruz, CA 95064
(831) 459-2477
jmkrupp-at-ucsc.edu

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From: cervantes-at-bendres.com
Date: Tue, 9 Jan 2007 10:51:07 -0600
Subject: [Microscopy] Cryo TEM Imaging of Nanoparticles

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

This is somewhat of a solicitation for information from the cryo TEM community. I'm imaging small (small = less than 20 nm) polymeric nanoparticles with cryo TEM, and as always, am striving for the best image possible. I'd like to obtain good (with respect to characteristics such as resolution and signal/noise ratio) images of small, low contrast, spherical particles, to serve as comparisons for images I generate . Journal and book references would be gladly appreciated. Conventional TEM images, with or without staining, would also be very helpful.

Jessica Cervantes
Research Chemist
Bend Research, Inc
64550 Research Rd
Bend, OR 97701
(541) 382-4100
(541) 382-0212 x240
(541) 382-6177 fax

LEGAL NOTICE: This message (and/or any attachments accompanying it) is confidential and proprietary. It is intended for the addressee(s) only. Access to this e-mail by anyone else is unauthorized. If you are not an addressee any disclosure, copying, or distribution of the contents of this e-mail (and any attachments) or any action taken (or not taken) in reliance upon this message is unauthorized and may be unlawful. If you are not an addressee, please contact the sender immediately by calling (541) 382-4100.



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From: m.aindow-at-uconn.edu
Date: Tue, 9 Jan 2007 14:31:49 -0600
Subject: [Microscopy] Postdoctoral Position

Contents Retrieved from Microscopy Listserver Archives
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The following is a re-posting of a vacancy that was advertised originally in
September, but was not filled due to an administrative re-organization.

****************************************************************************

University of Connecticut
Institute of Materials Science

Position in Scanning Electron Microscopy and Optical Microscopy


The Institute of Materials Science (IMS) at UConn is an interdisciplinary
center with the threefold mission of fostering education, research and
outreach in all areas of the materials sciences. The Microscopy Laboratory
is a user facility which houses the main research microscopes (optical, SEM
and TEM) for the IMS. There is an opening in the Laboratory for a Scanning
Electron Microscopy / Optical Microscopy specialist. The appointee will be
involved in a range of academic and industrial projects, and will assist in
the operation of the Laboratory including performing routine maintenance and
training/assisting users of the microscopes.

Candidates should hold a higher degree (MS or PhD) in Materials Science or a
related discipline and must have extensive hands-on SEM and optical
microscopy experience. Experience in maintenance of electron microscopes,
use of transmission electron microscopy, microscopy of soft materials and/or
microtomy would also be beneficial. This is a fixed-term appointment and is
available from February 1st. Screening of the applications will begin
immediately and will continue until the post is filled. Applications from
under-represented groups, including minorities, women and persons with
disabilities are encouraged.

Interested candidates should send a curriculum vitae and the names of at
least three referees with postal addresses, telephone numbers and Email
addresses to: Prof. Mark Aindow, Institute of Materials Science, University
of Connecticut, 97 North Eagleville Road, Unit 3136, Storrs, CT 06269-3136
USA. Email: m.aindow-at-uconn.edu





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From: ani.issaian-at-csun.edu
Date: Tue, 9 Jan 2007 18:13:46 -0600
Subject: [Microscopy] Re: Cryo TEM Imaging of Nanoparticles

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Jessica,

Try staining the polymeric sections/particles with OsO4 at different times, ie., 5, 7, 10min, look at them with the TEM and select the best results.

Ani

---- Original message ----
} Date: Tue, 9 Jan 2007 11:03:32 -0600
} From: cervantes-at-bendres.com
} Subject: [Microscopy] Cryo TEM Imaging of Nanoparticles
} To: ani.issaian-at-csun.edu
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

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From: connellyps-at-mail.nih.gov
Date: Tue, 9 Jan 2007 19:35:00 -0600
Subject: [Microscopy] AskAMicroscopist: fixation for mitochondria

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This Question was submitted to Ask-A-Microscopist by (connellyps-at-mail.nih.gov)
from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Tuesday, January 9, 2007 at 17:33:37
Remember to consider the Grade/Age of the student when considering the Question
---------------------------------------------------------------------------
Please reply to both connellyps-at-mail.nih.gov as well as to the Microscopy Listserver
---------------------------------------------------------------------------

Email: connellyps-at-mail.nih.gov
Name: Pat Connelly

Organization: NIH

Education: Graduate College

Location: Bethesda, MD

Title: fixation for mitochondria

Question: Dear Fellow Experts,

I am in need of the "best" fixation for mitochondria in mouse and rat muscle and kidney. Past experiments have yeilded OK but not great results using 2.5%glut with and without paraformaldehyde in sodium cacodylate buffer followed by 1% OsO4 and UA block stain. I'd like to be saying "Wow, Look at these!"
Any suggestions or references? Today I did a Pub Med search and most of what I found was the same as I have been doing.

Thanks,
Pat Connelly
connellyps-at-mail.nih.gov

---------------------------------------------------------------------------

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From: WAHeeschen-at-dow.com
Date: Wed, 10 Jan 2007 07:53:49 -0600
Subject: [Microscopy] Job Posting: Microscopy technologist for Dow Chemical in Freeport

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Microscopy Technologist Position at The Dow Chemical Company in Freeport, Texas

The Dow Chemical Company, an equal opportunity employer, is seeking applications for an immediate opening for a microscopy technologist. Candidates should have training in light and electron microscopies and related sample preparation methods. The applicant should have a minimum of an Associate Degree in Science. The successful applicant will work under the direction of a Dow Researcher to deliver microscopy related solutions to real world industrial problems. The job focus will be primarily related to microscopy of polymers including; light microscopy, scanning electron microscopy and transmission electron microscopy and soft material sample preparation to include; ultramicrotomy and cryo-ultramicrotomy. The position will be at Dow's Freeport, Texas Analytical Laboratory, which houses modern LM, SEM, TEM, SPM and surface science equipment.

The salary is commensurate with experience. Please send hardcopy resume' to:
Dr. John Blackson
Building 1897 E29C
The Dow Chemical Company
Midland, MI 48667

Electronic resume' can be sent to
tdominowski-at-dow.com


Best Regards,

Bill
William A. Heeschen, Ph.D.
Building 1897 E84
The Dow Chemical Company
Midland, MI 48667
waheeschen-at-dow.com

Dow is a leader in science and technology, providing innovative chemical, plastic and agricultural products and services to many essential consumer markets. With annual sales of $40 billion, Dow serves customers in 175 countries and a wide range of markets that are vital to human progress: food, transportation, health and medicine, personal and home care, and building and construction, among others. Committed to the principles of sustainable development, Dow and its 43,000 employees seek to balance economic, environmental and social responsibilities. References to "Dow" or the "Company" mean The Dow Chemical Company and its consolidated subsidiaries unless otherwise expressly noted.

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From: zhangj16-at-msu.edu
Date: Wed, 10 Jan 2007 14:04:17 -0600
Subject: [Microscopy] Hitachi H800 vacuum problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear everyone

I had problems with Hitachi H800 TEM vacuum sequences now. From start up of
Evac, three rough pumps(RP) start to work. RP1 and 2 pump the valve of two
diffusion pump to open. RP3 cause the pre evac and a valve of column to
open. Then the vacuum level of column and pre evac are just stop at around
8*10^-1 pa, no further progress can be reach.

Please share your idea what may be the problems. Thanks.


Jiaming Zhang

Research Assistant
Department of Chem. Eng. & Mater. Sci. Michigan State University
Tel: 517-231-8013
Email: zhangj16-at-egr.msu.edu


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From: bigelow-at-engin.umich.edu
Date: Wed, 10 Jan 2007 15:13:37 -0600
Subject: [Microscopy] RE: H800 vacuum problem

Contents Retrieved from Microscopy Listserver Archives
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It sounds as though your vacuum system has basically the same
configuration as the one shown in Figure 9.5 of my book, Vacuum
Methods in Electron Microscopy (available from SPI Supplies, M. E.
Taylor, Ladd, etc.), except that you have diffusion pumps rather than
the turbomolecular pumps shown in this figure. The discussion given
of the operation of the system in this figure should give you a
pretty good understanding of how your system should operate.

In particular, I suspect that RP3 on your system might serve two
functions: 1. it might be used to rough pump the column during an
initial pump down operation, and 2. it probably serves to evacuate
the specimen chamber during specimen exchange operations. If these
assumptions are correct then the valves between RP3 and the column
should open during the initial stages of pumpdown, but once a
suitable vacuum (about 10-1 Pa) is reached in the column the valves
between RP3 and the column should close and remain closed, except
during specimen change operations. You might want to check the
operation of these valves on your system to be sure that they are
operating properly. If they do not close when they should they could
cause the column pressure to remain too high, as you describe. If
you check with your department's electrician he will have a gadget
that he can simply clamp around the wires leading to these valves
that will read the current flowing to them, and thus be able to
determine when they are activated and when they are not.

If the problem does not involve the RP3 system, then there must be
something wrong with the diffusion pumps. Either the valves between
them and the column are not opening as they should or the diffusion
pumps themselves are not working properly. There are many potential
problems with diffusion pumps, too many to discuss here; however,
most of them are discussed in considerable detail in Section 5.8 (p.
214) of my book. First check to be sure the valves in these systems
are operating properly, next check to be sure the DPs are getting
proper electrical power , then check that the flow of cooling water
is as it should be. Beyond that you have a really serious problem and
probably should call in the Hitachi service representative.

Good luck,

--
Wilbur C. Bigelow, Professor Emeritus
Materials Sci. & Engr., Univ. of Michigan
Ann Arbor, Michigan 48109-2136
e-mail: bigelow-at-engin.umich.edu;
Fx:734-763-4788; Ph:734-975-0858
Address mail to: 2911 Whittier Court
Ann Arbor, MI 48104-6731

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From: emlabservices-at-cox.net
Date: Wed, 10 Jan 2007 16:03:42 -0600
Subject: [Microscopy] Hitachi H800 vacuum problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hello Jiaming,

The vacuum level stated in your message indicates only roughing pressure. I
would check to see if both diffusion pump heaters are hot (don't touch these
directly with your hand; see if you can sense warmth by holding your hand an
inch or so away from the base of the pump), the sides of the diffusion pumps
are cool (confirming you have adequate cooling water; again be cautious),
and if all this seems ok, clean your penning head as the vacuum system
sequence relies on this reading to continue on to high vacuum pumping and
appropriate valving. I think your microscope instruction manual details a
way to do this cleaning.

Good luck !

Bob Roberts
EM Lab Services, Inc.
449 NW 62nd St.
Topeka, Kansas 66617-1780
785.246.1232 voice
785.246.0168 fax
www.emlabservices.com



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From: smiller-at-umr.edu
Date: Wed, 10 Jan 2007 17:13:27 -0600
Subject: [Microscopy] TEM postdoctoral position

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Post-Doctoral Fellowship in TEM and Materials Characterization
Materials Science and Engineering Department
University of Missouri-Rolla

The University of Missouri-Rolla, Department of Materials Science and Engineering, is seeking candidates for a postdoctoral fellowship position in transmission electron microscopy of thin films and hard materials. In addition to experience in TEM sample preparation and analysis, the ability to utilize other materials characterization techniques such as Auger and x-ray photoelectron spectroscopies and scanning electron microscopy is also desired. Experience in the characterization of interfaces is also beneficial, though not required. Candidate must be able to work independently and in a group, have excellent communication skills, and provide guidance to team members. The project is a part of a multi-university research initiative on diamond coatings.

A Ph.D. degree in materials science or a related field is preferred; persons with a MS degree may be acceptable with extensive background in TEM. A minimum of 2 years of experience operating a TEM, analyzing images and electron diffraction results, and preparation of TEM samples is required.

Interested candidates should send a Resume/Curriculum Vitae, Letter of Intent, and References to: Prof. Matt O'Keefe, 302 Materials Research Center, University of Missouri-Rolla, Rolla, MO 65409 USA. Email: mjokeefe-at-umr.edu




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From: johnf-at-geology.wisc.edu
Date: Thu, 11 Jan 2007 05:16:37 -0600
Subject: [Microscopy] HKL ebsd image question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

If anyone on the list is using the HKL ebsd system, please contact me
at johnf-at-geology.wisc.edu
I have a question regarding the quality of backscatter vs forescatter
image display from the detectors sitting above and below the camera.

Thanks.

John

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From: nizets2-at-yahoo.com
Date: Thu, 11 Jan 2007 09:05:43 -0600
Subject: [Microscopy] Re: AskAMicroscopist: fixation for mitochondria

Contents Retrieved from Microscopy Listserver Archives
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Dear Pat,

If what you mean by "best fixation" is a fixation
which does not allow artifacts or difformations of the
structure, apart from cryo-fixation I think that your
fixation is optimal (and is pretty classical).
Personally I have a preference for the addition of a
low concentration of formaldehyde (2%) because it
penetrates faster than glutar in the tissue. It acts
as a sort of "pre-fixative" before the glutar comes
and stabilizes the structures.

If you mean "increasing contrasting of the membranes",
you could consider using ferrocyanide (which I tried
successfully) and/or tannic acid (which I didn't try
but is well documented).

I found this reference for you but there are probably
others:
Berryman MA, et al (1992) Effects of tannic acid on
antigenicity and membrane contrast in ultrastructural
immunocytochemistry. J Histochem Cytochem 40, 6,
845-857

Regards or LG as they say here in Austria

Stephane


--- connellyps-at-mail.nih.gov wrote:

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} Email: connellyps-at-mail.nih.gov
} Name: Pat Connelly
}
} Organization: NIH
}
} Education: Graduate College
}
} Location: Bethesda, MD
}
} Title: fixation for mitochondria
}
} Question: Dear Fellow Experts,
}
} I am in need of the "best" fixation for mitochondria
} in mouse and rat muscle and kidney. Past experiments
} have yeilded OK but not great results using 2.5%glut
} with and without paraformaldehyde in sodium
} cacodylate buffer followed by 1% OsO4 and UA block
} stain. I'd like to be saying "Wow, Look at these!"
} Any suggestions or references? Today I did a Pub
} Med search and most of what I found was the same as
} I have been doing.
}
} Thanks,
} Pat Connelly
} connellyps-at-mail.nih.gov
}
}
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From: mcauliff-at-umdnj.edu
Date: Thu, 11 Jan 2007 09:29:07 -0600
Subject: [Microscopy] Re: fixation for mitochondria

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Pat:

I agree with Stephane, there is nothing wrong with the fixative you
are using. If more contrast is what you seek you could also try a
post-fix or mordant in potassium dichromate. Substituting Dalton's
chrome-osmium fix for your regular osmium post-fix might be worth a try.
Alternatively, you could try a "light" fix followed by
histochemistry to demo mitochondrial enzymes, they post fix with osmium.
This would be more work.

Geoff

connellyps-at-mail.nih.gov wrote:

}
} Email: connellyps-at-mail.nih.gov
} Name: Pat Connelly
}
} Organization: NIH
}
} Education: Graduate College
}
} Location: Bethesda, MD
}
} Title: fixation for mitochondria
}
} Question: Dear Fellow Experts,
}
} I am in need of the "best" fixation for mitochondria in mouse and rat muscle and kidney. Past experiments have yeilded OK but not great results using 2.5%glut with and without paraformaldehyde in sodium cacodylate buffer followed by 1% OsO4 and UA block stain. I'd like to be saying "Wow, Look at these!"
} Any suggestions or references? Today I did a Pub Med search and most of what I found was the same as I have been doing.
}
} Thanks,
} Pat Connelly
} connellyps-at-mail.nih.gov
}
} ---------------------------------------------------------------------------
}
} ==============================Original Headers==============================
} 10, 12 -- From zaluzec-at-ultra5.microscopy.com Tue Jan 9 19:35:00 2007
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} 10, 12 -- From: connellyps-at-mail.nih.gov (by way of Ask-A-Microscopist)
} 10, 12 -- Subject: AskAMicroscopist: fixation for mitochondria
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--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff-at-umdnj.edu
**********************************************



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From: sshroff-at-bmrn.com
Date: Thu, 11 Jan 2007 18:16:32 -0600
Subject: [Microscopy] viaWWW: peptide diffusion

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Email: sshroff-at-bmrn.com
Name: Shilpa

Organization: BioMarin

Title-Subject: [Filtered] peptide diffusion

Question: Hi -

I am looking at antigen presentation on the surface of a cell line. I am first incubating the cell with a protein (for about 2hrs). The cells are fixed after the incubation in 4% paraformaldehyde, permeabilized in TritonX.

If my protein were fragmented, would I have to worry about loosing the small peptides even though I fixed in paraformaldehyde? I am staining the protien with a fluorescent polyclonal to check its internalization

thanks
-shilpa

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From: phillipst-at-missouri.edu
Date: Thu, 11 Jan 2007 18:23:19 -0600
Subject: [Microscopy] Re: viaWWW: peptide diffusion

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One other concern would be that your protein was cross-linked to another
protein that was then extracted by the Triton X-100.

At 06:17 PM 01/11/07, you wrote:



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From: almecid-at-tcd.ie
Date: Fri, 12 Jan 2007 08:08:48 -0600
Subject: [Microscopy] viaWWW: Carbon Grids and Furnace

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Email: almecid-at-tcd.ie
Name: Dorothee

Organization: Trinity College Dublin

Title-Subject: [Filtered] TEM grids

Question: Can carbon TEM grids withstand 450 degrees in a furnace?


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From: phillipst-at-missouri.edu
Date: Fri, 12 Jan 2007 10:00:55 -0600
Subject: [Microscopy] Photoshop question

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I consider myself pretty skilled in Photoshop but there is one simple thing
I don't know how to do and it drives me crazy. I have searched the
Photoshop online help and other websites to no avail. I want to keep the
images anchored to the Photoshop screen frame. The problem is that I start
to zoom an image and it grows to cover the menu bar. This makes subsequent
manipulation tough. I am sure there is just some simple thing to toggle but
I can't find it. I use Photoshop CS2 (version 9). thanks for any tips you
have. tom



Thomas E. Phillips, PhD
Professor of Biological Sciences
Director, Molecular Cytology Core
2 Tucker Hall
University of Missouri
Columbia, MO 65211-7400

573-882-4712 (office)
573-882-0123 (fax)
PhillipsT-at-missouri.edu
http://www.biology.missouri.edu/faculty/phillips.html
http://www.biotech.missouri.edu/mcc/


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From: DrJohnRuss-at-aol.com
Date: Fri, 12 Jan 2007 10:05:40 -0600
Subject: [Microscopy] Re: Photoshop question

Contents Retrieved from Microscopy Listserver Archives
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Select the magnifying glass icon. In the options bar that appears
across the top of your screen, uncheck the "resize images to fit"
item. When you zoom the image the window in which it appears will not
change size or position.


On Jan 12, 2007, at 11:01 AM, phillipst-at-missouri.edu wrote:

}
}
}
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} I consider myself pretty skilled in Photoshop but there is one
} simple thing
} I don't know how to do and it drives me crazy. I have searched the
} Photoshop online help and other websites to no avail. I want to
} keep the
} images anchored to the Photoshop screen frame. The problem is that
} I start
} to zoom an image and it grows to cover the menu bar. This makes
} subsequent
} manipulation tough. I am sure there is just some simple thing to
} toggle but
} I can't find it. I use Photoshop CS2 (version 9). thanks for any
} tips you
} have. tom
}
}
}
} Thomas E. Phillips, PhD
} Professor of Biological Sciences
} Director, Molecular Cytology Core
} 2 Tucker Hall
} University of Missouri
} Columbia, MO 65211-7400
}
} 573-882-4712 (office)
} 573-882-0123 (fax)
} PhillipsT-at-missouri.edu
} http://www.biology.missouri.edu/faculty/phillips.html
} http://www.biotech.missouri.edu/mcc/
}
}
}




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From: TindallR-at-missouri.edu
Date: Fri, 12 Jan 2007 10:18:55 -0600
Subject: [Microscopy] TEM: Infiltration of lens

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Dear Listers,

Does anyone have a protocol for effective infiltration of lenses encased
in retinas? Pretty please?? We have tried extended infiltration times
(up to three days) and microwave-assisted infiltrations with Epon/Spurrs
and pure Spurrs and have still not achieved good infiltration. There
are holes on either side of the retinas to help solutions move in and
out, but the lenses are still crumbling during sectioning. The retinas
are okay.

I just know somebody's out there who can help with this......

Thanks much.

Randy

Randy Tindall
Senior EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W125 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu
On-line calendar:
http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=
Week&NavType=Both&Type=TimePlan


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From: RFleck-at-nibsc.ac.uk
Date: Fri, 12 Jan 2007 10:36:24 -0600
Subject: [Microscopy] Postdoctoral Research Opportunity at NIBSC

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National Institute for Biological Standards and Control

Hertfordshire, UK

Post-doctoral Researcher

Division of Cell Biology and Imaging

As part of a DTI initiative in Regenerative Medicine Technologies a three year post-doctoral research position has become available. The project is part of a consortium bringing together strengths in advanced imaging, robotic microwell-based engineering and combinatorial techniques. The programme aims to create a leading technology for quickly and efficiently examining large numbers of linked variables, addressing a central issue of regenerative medicine; the capacity to reproducibly control the differentiation of a high proportion of stem cells.
The successful candidate will undertake studies to investigate differentiation of cells and propose solutions to the technical challenges for scaling the patented concept. Work will require development and study of differentiation systems . Interaction between cells, the cells and carrier beads, effects of culture conditions, environment, differentiation media and the functional capacity of cells in biological assays will be considered.
We are seeking an enthusiastic post-doctoral scientist capable of pursuing productive research in molecular cell biology. You will have a Life Science PhD or appropriate subject with at least 3 years experience in microscopy in a research environment. It is essential you have experience of confocal and/or electron microscopy. In addition, you will require experience of one or more: immuno-gold/fluorescent, confocal, live cell, cryo-SEM or cryo-TEM techniques. You will be based in the recently refurbished imaging facility equipped with new high resolution electron microscopes and supporting specimen preparation equipment.

Salary will be within Pay Band E on a scale from £24,815 up to £28,478, dependent on experience.

Please see our website www.nibsc.ac.uk for a full job description.

To apply, please submit a full Curriculum Vitae (2 copies), including the names and addresses of two referees, to the Human Resources Department, National Institute for Biological Standards and Control, Blanche Lane, South Mimms, Potters Bar, Hertfordshire, EN6 3QG , or email HR-at-nibsc.ac.uk
Closing date for applications is 1 February 2007.
Please quote reference SU.CB.567 clearly on your application.




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From: PAGE.BALUCH-at-asu.edu
Date: Fri, 12 Jan 2007 11:41:28 -0600
Subject: [Microscopy] TEM artifact ?

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Hi, I am new at using the TEM and preparing my adherent culture cells for
this type of imaging so am hoping that someone may have experience with this
unusual artifact that showed up in some of my cells. I had 2 sets of cells.
One set was untreated and the others were treated with membrane permeate
pseudosubstrate peptides and or nocodazole. The cells that were not treated
in any way appeared normal upon imaging but the cells that had been treated
had fiber-like artifacts throughout the cytoplasm and nuclei. The "fibers"
were localized mainly within the cells and appeared to be consistently
0.1umin length. I am assuming the artifact is originating from the
peptides but was wondering if anyone else has had this experience. I also
repeated the post staining process with new sections to rule out any
problems with lead
citrate or uranyl acetate precipitants but had the same problem. Thanks for
any advice.
Page

Page Baluch
Arizona State University/SoLS
P.O. Box 4601
Tempe, AZ 85287-4601
Lab: 480-965-7011
Fax: 480-965-7599


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From: tivol-at-caltech.edu
Date: Fri, 12 Jan 2007 11:45:49 -0600
Subject: [Microscopy] Re: viaWWW: Carbon Grids and Furnace

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On Jan 12, 2007, at 6:08 AM, almecid-at-tcd.ie wrote:

} Question: Can carbon TEM grids withstand 450 degrees in a furnace?
}
Dear Dorothee,
Carbon can certainly withstand 450 C, so if the grid itself is made of
carbon, the answer should be "yes", assuming that there are no problems
with heating or cooling at a rate such that thermal stresses are not
introduced. Carbon films on metal grids could be a different story,
however. The large difference between the coefficients of expansion of
metal and carbon will very likely cause problems.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu


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From: ian.anderson-at-nist.gov
Date: Fri, 12 Jan 2007 17:59:40 -0600
Subject: [Microscopy] viaWWW: Microbeam Analysis Society (MAS) 2007 Topical Workshop

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Email: ian.anderson-at-nist.gov
Name: Ian Anderson

Organization: NIST

Title-Subject: [Filtered] Microbeam Analysis Society (MAS) 2007 Topical Workshop

Question: Microbeam Analysis Society (MAS)
2007 Topical Workshop
Hyperspectral Imaging II
Advanced Measurement Laboratory (AML)
May 1 ñ 4, 2007 ? NIST, Gaithersburg, MD, USA


Special provisions for students and Special provisions for students and postdocs postdocs!


http://www.microbeamanalysis.org/workshops/HI /workshops/HI-II

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From: cosandey-at-rci.rutgers.edu
Date: Fri, 12 Jan 2007 18:02:20 -0600
Subject: [Microscopy] viaWWW: PostDoc Research Associate Position

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Email: cosandey-at-rci.rutgers.edu
Name: Fred Cosandey

Organization: Rutgers University

Title-Subject: [Filtered] PostDoc Research Associate Position

Question: Research Associate Position
Transmission Electron Microscopy

The Department of Materials Science and Engineering at Rutgers University is currently seeking a candidate for a Research Associate position in Transmission Electron Microscopy.
The successful candidate should have a PhD degree in Materials Science or related discipline and have extensive experience in the use of transmission electron microscope including HAADF, GIF and EELS spectroscopy techniques. Prior experience with JEOL 2010F STEM would be preferable.
Specific projects involve STEM analysis of nanostructured electrode materials used in Li-Ion batteries, including EELS determination of valence state and structural as well as chemical analysis during Li-induced phase transformations. Also, chemical and electronic structure determination of interfaces in oxides and carbide systems.

The appointment will be for one year and may be renewable for subsequent years. Interested candidate should send their CV with three references to:

Professor Frederic Cosandey
Department of Materials Science and Engineering
Rutgers University
607 Taylor Rd.
Piscataway, NJ 08854-8065,
cosandey-at-rci.rutgers.edu, 732-445-4942 (phone), 732-445-5977 (fax)

Rutgers University is an equal opportunity/affirmative action employer.



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From: johnf-at-geology.wisc.edu
Date: Sat, 13 Jan 2007 03:33:37 -0600
Subject: [Microscopy] Re: Microbeam Analysis Society (MAS) 2007 Topical Workshop

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Re the MAS 2007 Topical Workshop on Spectral Imaging:
if the previous url did not work, try
http://www.microprobe.org/workshops/HI-II/

sdf

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From: michael-at-shaffer.net
Date: Sat, 13 Jan 2007 06:09:16 -0600
Subject: [Microscopy] RE: viaWWW: Microbeam Analysis Society (MAS) 2007 Topical Workshop

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

The correct link would be:

http://www.microprobe.org/workshops/HI-II/


} Special provisions for students and Special provisions for
} students and postdocs postdocs!
}
}
} http://www.microbeamanalysis.org/workshops/HI /workshops/HI-II
}


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From: jl523136-at-gmail.com
Date: Sun, 14 Jan 2007 10:11:42 -0600
Subject: [Microscopy] viaWWW: EXELFS analysis to obtain RDF

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Email: jl523136-at-gmail.com
Name: Juntao Li

Organization: NY

Title-Subject: [Filtered] EXELFS analysis to obtain RDF

Question: Hi, everybody,
I have a question about the EXELFS analysis.
I try to use EXELFS analysis to obtain radial distribution function of amorphous materials.
I followed the standard procedure,
1. Acquisition of high quality edge, deconvolution and background removal
2. Isolation of the oscillatory part of the intensity (normalization)
3. Scale conversion —(k) of into k space —(k)
4. Truncation of —(k)
5. Fourier distribution gives raw Radial Distribution Function (RDF)
6. Correction for phase shifts

} From step 4 to step 5 I was confused, I use Origin to the FFT, but in the image which I got, the X-axies is frequency, but in many papers I found that they got the RDF, where the x-axis is radial distance,the unit is angstrom or nm. I can not understand this, is there anybody can tell me how to get the RDF? Thanks a lot.




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From: nizets2-at-yahoo.com
Date: Mon, 15 Jan 2007 06:32:38 -0600
Subject: [Microscopy] Re: viaWWW: peptide diffusion

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Shilpa,

Although I have no direct experience to answer this
question, on the basis of what is known about PFA
fixation I would say there is a chance you lose a
non-negligible part of your peptides.
Do you retain enough to allow immunodetection is a
question you can only answer by trying. A negative
signal will be hard to interpret, though, because it
could indeed come from this technical limitation.
If I were you, I'd include as a control cells
incubated with your protein at 4°C, this would allow
the protein to bind its receptor but would block the
internalization and subsequent processing (if you can
block the processing after internalization it is even
better, but you have to prove that the processing is
actually blocked - with a gel for example). Your
antibody should recognize the peptide sequence in the
context of the full protein (?) so this would allow
you to confirm that the immunodetection actually
works.

Good luck,

Stephane

--- sshroff-at-bmrn.com wrote:

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}
} Email: sshroff-at-bmrn.com
} Name: Shilpa
}
} Organization: BioMarin
}
} Title-Subject: [Filtered] peptide diffusion
}
} Question: Hi -
}
} I am looking at antigen presentation on the surface
} of a cell line. I am first incubating the cell with
} a protein (for about 2hrs). The cells are fixed
} after the incubation in 4% paraformaldehyde,
} permeabilized in TritonX.
}
} If my protein were fragmented, would I have to worry
} about loosing the small peptides even though I fixed
} in paraformaldehyde? I am staining the protien with
} a fluorescent polyclonal to check its
} internalization
}
} thanks
} -shilpa
}
}
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} Headers==============================
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} 9, 12 -- From: sshroff-at-bmrn.com (by way of
} MicroscopyListserver)
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____________________________________________________________________________________
Food fight? Enjoy some healthy debate
in the Yahoo! Answers Food & Drink Q&A.
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From: kim.kiely-at-hrx.com.au
Date: Tue, 16 Jan 2007 01:01:17 -0600
Subject: [Microscopy] Atom Probe Engineer at the University of Sydney

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Atom Probe Engineers - 2 positions available
Electron Microscope Unit at the University of Sydney, Australia

The Electron Microscope Unit (www.emu.usyd.edu.au) is located on the
main campus of the University of Sydney and incorporates the Australian
Key Centre for Microscopy and Microanalysis as its research and training
arm. The Key Centre is a research leader in the field and has some of
the most advanced facilities in the world for microscopy and
microanalysis. As such, it seeks to develop techniques, expertise and
understanding of the relationships between the structure and function of
physical, chemical, biological and other systems. It also aims to
provide leadership in the development of innovation and ingenuity in
Australian science and engineering, and is seeking to recruit two
outstanding Atom Probe Engineers to advance this aim.

The Atom Probe Engineers will provide the national user community with
technical support for research using atom probe tomography, particularly
as the Key Centre becomes the headquarters of a new National Microscopy
and Microanalysis Research Facility during 2007. The Engineers will work
closely with the Director and all unit staff to assure the success of
the centre's research services and programs, and their integration with
the new research facility, including national and international
collaborations. They will provide technical support to users during
their training and operation of the existing advanced atom probe and the
forthcoming laser atom probe platform.

In this position, the Engineers will create solutions to specimen
fabrication, determine sound running conditions for data acquisition,
and conduct data reconstruction and analysis. So far as is possible, we
prefer to train users in the relevant areas. In addition, there is scope
for the appointees to undertake their own research in collaboration with
the Director, and leading edge projects are available for immediate
commencement. These exciting positions will require a commitment to
technical excellence and will expose the appointees to cutting-edge
research and development in nanotechnology. The advanced atom probe
platforms are Imago LEAP(tm) instruments (www.imago.com).

The Engineers will have exceptional communication, interpersonal and
problem solving skills, and the capacity to operate sophisticated
scientific instrumentation. Skills in MS Office, email and internet will
be essential, as will strong organisational and presentation skills and
the ability to work both independently and in a team. In addition, the
appointees will be committed to understanding and servicing the
technical requirements of users, and be willing to undertake national
and international travel.

To succeed, the appointees will have a Bachelor degree with Honours, and
a background in applied physics, computer systems engineering,
electronic engineering, mechanical/mechatronic engineering or materials
engineering. A PhD in these areas will be highly advantageous.

These positions are full-time fixed term for five years, subject to the
completion of a satisfactory probation period for new appointees.
Following the initial five years, longer term appointments may be
possible subject to available funding and facility demand. Membership of
a University-approved superannuation scheme is a condition of employment
for new appointees.

Remuneration package: AU$57,161- $64,050 p.a. (which includes a base
salary Level 5 $48,302 - $54,123 p.a., leave loading and up to 17%
employer's contribution to superannuation)

Depending on expertise, skills and qualifications, the remuneration
package can be potentially higher than Level 5. This will be negotiated
with quality candidates with relevant experience.

Closing: 2 February 2007

All applications must be completed online at the following url:
http://positions.usyd.edu.au, search by position reference number
92079. Specific enquiries about the role can be directed to Kim Kiely on
+61 2 9561 9068 or k.kiely-at-usyd.edu.au.



Kim Kiely
Strategic Sourcing Specialist

E kim.kiely-at-hrx.com.au
T 02 9561 9068
ABN 97 116 399 690
Level 6, 213 Miller Street, North Sydney, NSW 2060





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From: kim.kiely-at-hrx.com.au
Date: Tue, 16 Jan 2007 01:03:08 -0600
Subject: [Microscopy] Nanostructural Analysis of Steels

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Research Associate - Nanostructural Analysis of Steels
The Australian Key Centre for Microscopy and Microanalysis

The Australian Key Centre for Microscopy and Microanalysis
(www.emu.usyd.edu.au) intends to appoint an outstanding Research
Associate to conduct excellent research on a project to be conducted in
collaboration with BlueScope Steel (www.bluescopesteel.com.au). The
Research Associate will be based at the University of Sydney, and work
closely with the staff at the BlueScope Steel Research Centre in
Wollongong. The project contains scope for international travel in the
form of visits to the United States for production trials and attendance
at international conferences worldwide.

Castrip(r) is a revolutionary new method of producing steel strip
directly from liquid steel that BlueScope Steel plan to commercialise in
Australia. Currently, only plain carbon steels are produced using this
method. This project will investigate microalloying additions known to
be highly effective in conventional steels with an emphasis on nanoscale
microstructure using advanced microscopy techniques including
transmission electron microscopy (TEM) and atom probe tomography (APT).
For the first time, we will explore and optimise the effect of these
alloying additions in strip cast steels where dramatically higher
cooling rates and special deoxidation practices present opportunities
for innovative new design of steel nanostructure. Ultimately we will
perform production trials of the most promising alloys with a view to
developing design concepts for a new class of strip cast steels.

Located on the University's Camperdown campus, the Key Centre for
Microscopy and Microanalysis headquarters the National Microscopy and
Microanalysis Research Facility and is a major node of the ARC Centre of
Excellence for Design in Light Metals. Researchers have access to an
outstanding array of nanostructural analysis equipment, both within the
Unit and at our partner nodes.

The successful applicant will be expected to engage in all aspects of
the academic life of the facility. This includes approved collaboration
with facility users, other facility staff and approved involvement with
training activities. In this way, excellent opportunities for an
academically-rich experience are available through this position. In
addition, the appointee will be expected to write quality manuscripts
that are suitable for publication in peer-reviewed journals.

To succeed, a recent PhD in metallurgy, materials science, engineering,
physics, chemistry or a related discipline is essential. The position is
full-time fixed term for 2 years, subject to the completion of a
satisfactory probation period for new appointees. There is the
possibility of further offers of employment for 12 months, subject to
funding and need. Membership of a University approved superannuation
scheme is a condition of employment for new appointees. The appointment
can only commence after the Collaborating Organisation Agreement is
signed with the BlueScope Steel.

Remuneration package: AU$72,327 - $77,638 p.a. (which includes a base
salary Level A $61,117 - $65,605 p.a., leave loading and up to 17%
employer's contribution to superannuation)

Closing: 2nd February 2007

All applications must be completed online at the following url:
http://positions.usyd.edu.au and search by position reference number,
92070. Specific enquiries about the role can be directed to Kim Kiely on
+61 2 9561 9068 or k.kiely-at-usyd.edu.au.









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From: kim.kiely-at-hrx.com.au
Date: Tue, 16 Jan 2007 01:05:39 -0600
Subject: [Microscopy] Research Associate in Advanced Microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Research Associate - Advanced Microscopy
The Australian Key Centre for Microscopy and Microanalysis

The Australian Key Centre for Microscopy and Microanalysis
(www.emu.usyd.edu.au) currently has an outstanding opportunity available
for a results-driven Research Associate to conduct specialised research
investigating grain boundary chemistry using state-of-the-art microscopy
and microanalysis techniques.

Located on the University's Camperdown campus, the Key Centre hosts
world-class facilities, and is renowned for its research excellence. The
Centre headquarters the National Microscopy and Microanalysis Research
Facility and is a major node of the ARC Centre of Excellence for Design
in Light Metals. Researchers have access to an outstanding array of
nanostructural analysis equipment, both within the Unit and at our
partner nodes.

The appointee will be involved in the study of the relationship between
solute atmospheres at grain boundaries and the rate of grain boundary
migration during annealing in practically important alloy systems. He or
she will lead in the development of advanced experimental techniques
utilising Focused Ion Beam (FIB) technology, Transmission Electron
Microscopy (TEM) and Atom Probe Tomography (APT). This research project
will be carried out in collaboration with Imago Scientific Instruments
(www.imago.com), and contains scope for international travel in the form
of research visits to the United States and attendance at international
conferences worldwide.

The Key Centre attracts high-profile scientists and research groups, so
an ability to communicate and work with others will be crucial. In
addition, the appointee will need an ability to write quality
manuscripts that are suitable for publication in peer-reviewed journals.

To succeed, a recent PhD in materials science, engineering, physics,
chemistry or a related discipline is essential. This is the perfect
opportunity for a recent graduate to conduct innovative research in a
specialised field that attracts significant research coverage and
funding.

The position is full-time fixed term for 2 years, subject to the
completion of a satisfactory probation period for new appointees. There
is the possibility of further offers of employment for 12 months,
subject to funding and need. Membership of a University approved
superannuation scheme is a condition of employment for new appointees.

Remuneration package: AU$72,327 - $77,638 p.a. (which includes a base
salary Level A $61,117 - $65,605 p.a., leave loading and up to 17%
employer's contribution to superannuation)

Closing: 2nd Febaury 2007

All applications must be completed online at the following url:
http://positions.usyd.edu.au and search by position reference number,
92070. Specific enquiries about the role can be directed to Kim Kiely on
+61 2 9561 9068 or k.kiely-at-usyd.edu.au.



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From: kim.kiely-at-hrx.com.au
Date: Tue, 16 Jan 2007 01:08:15 -0600
Subject: [Microscopy] Microscopy and Microanalysis at the University of Sydney

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Electron Microscope Unit (EMU)
Careers in Microscopy and Microanalysis

Established in 1958, The University of Sydney's Electron Microscope Unit (EMU) produces quality research services, leading research programs, and internationally recognised research training in microscopy and microanalysis. It serves as national headquarters for the NANO Major National Research Facility (see: www.nano.org.au). The Unit recognises the substantial role that microscopy and microanalysis is set to play in the next decade, as biotechnology and nanoscience have increasing impact on science and society.
The Unit has recently received significant competitive research grant funding and is undergoing major expansion. As a result, we are now seeking to recruit quality engineers and scientists with backgrounds in materials science and engineering, nanotechnology, physics, chemistry or other relevant engineering disciplines to undertake research in the Australian Key Centre for Microscopy & Microanalysis, the research and training arm of the EMU. We are presently mapping program and project requirements and have a number of positions to fill, including Postdoctoral Research Fellowships, Research Assistantships, Technical Staff Positions and PhD. Scholarships. A range of tenure in these positions is available, up to 5 years.
We are interested to hear from recent graduates at the Bachelor or PhD. level that have a passion for research and are interested in investigating structure-property relationships in advanced materials using state-of-the-art microscopy and microanalysis approaches. A common theme in this research is to understand the structure and dynamics of materials structure towards the atomic level, and to relate to the engineering design and performance of materials. Necessarily, this will involve advances in microscopy research and techniques as well as advances in design in materials. This research builds on national and international collaborations, so we are looking for researchers that are also willing to undertake travel and short secondments in the laboratories of our colleagues.

The projects relate to design in:

*    Light Alloys (alloys of Mg, Ti and Al)
*    Novel Steels Systems
*    Spintronic Systems
*    Other Nanomaterials

Demonstrated research potential is essential Research experience in relevant fields will be required in some of these positions. For more details on possible projects, see www.emu.usyd.edu.au and follow the "career" link. For more details on selection criteria, see the attachment "EOI - Generic Selection Criteria" below.

To register interest for one of these positions, follow the link http://positions.usyd.edu.au and search by position reference number 92067.  For enquiries about these roles please call Fabrice Noël on +61 2 9036 7295 or f.noel-at-usyd.edu.au.
Closing: Interested parties are urged to express interest as soon as possible to feed into the research mapping process, certainly by no later than Friday, 2 February, 2007.








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From: nizets2-at-yahoo.com
Date: Tue, 16 Jan 2007 03:14:48 -0600
Subject: [Microscopy] preparation of cells for SEM - question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear listers,

I have a request for experts in SEM who have some
experience with eukaryotic cells.
We have a basic SEM which is normally used to study
minerals, so we are not equipped with critical point
dryers and the like. Now I would like to have a look
at some cells in culture with the SEM but I have no
idea how I could prepare the cells without critical
point dryer. Of course if I simply fix and dehydrate
in acetone, then air dry the cells I expect the
structure to crumble. Well perhaps it won't give nice
pictures but perhaps it will be enough for what I want
to see. On the other hand perhaps I could keep them in
a semi-hydrated state and look at them at 10 Pa vaccum
(whatever "semi-hydrated state means ;-)).

I would be grateful if you cared to share your opinion
on this subjet with me.

Stephane



____________________________________________________________________________________
Bored stiff? Loosen up...
Download and play hundreds of games for free on Yahoo! Games.
http://games.yahoo.com/games/front

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From: David.Patton-at-uwe.ac.uk
Date: Tue, 16 Jan 2007 03:36:06 -0600
Subject: [Microscopy] preparation of cells for SEM - question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Stephane, I have always found your posts interesting so it is nice to be
able to help you. I think critical point drying is best for cell
preparation but we tend to use a hexamethyldisilazane (HMDS) dehydration
protocol as it is much easier.

I think you will find looking at wet cells problematic. With a tungsten
gun XL30 ESEM I find it very difficult to get adequate resolution with
cultured cells.

My HMDS protocol is given below. If anyone would like to suggest
improvements please fire away. Although fixing in PBS goes against EM
principals the results for modest magnification SEM are often good.

Dave


Preparation of dry samples for SEM using HMDS

Safety
Work in fume hood and use gloves
Retain ethanol and HMDS (hexamethyldisilazane) for disposal in
non-chlorinated waste bottle

Fixation

Fix in 4% glutaraldehyde in buffer (usually 0.1M )*
Leave for 1 hr at room temperature or 24hrs in the fridge
Rinse in buffer x3 (total storage time should exceed fixation time by a
factor of 3).

SEM Dehydration

5m in 20% ethanol
5m in 30% ethanol
5m in 50% ethanol
5m in 70% ethanol
5m in 90% ethanol
5m in 100% ethanol
5m in 100% ethanol


5m in 100% ethanol / HMDS (2:1)
5m in 100% ethanol / HMDS (2:2)
5m in 100% ethanol / HMDS (1:2)
5m in 100% HMDS
5m in 100% HMDS
5m in 100% HMDS


Remove specimen from HMDS and place on filter paper in a petri dish and
leave in fume hood until dry.

*For 4% glutaraldehyde in 0.1M buffer
Take, say, 50mls of 0.2M buffer, 34ml d water and 16ml of 25%
glutaraldehyde.

0.1M Phosphate buffer or sodium cacodylate are used.

Fixation Using PBS
8.4ml PBS
1.6ml glutaraldehyde

Gives 10ml of a 4% glutaraldehyde fixative




-----Original Message-----
X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com]
Sent: 16 January 2007 09:20
To: David Patton

Dear listers,

I have a request for experts in SEM who have some
experience with eukaryotic cells.
We have a basic SEM which is normally used to study
minerals, so we are not equipped with critical point
dryers and the like. Now I would like to have a look
at some cells in culture with the SEM but I have no
idea how I could prepare the cells without critical
point dryer. Of course if I simply fix and dehydrate
in acetone, then air dry the cells I expect the
structure to crumble. Well perhaps it won't give nice
pictures but perhaps it will be enough for what I want
to see. On the other hand perhaps I could keep them in
a semi-hydrated state and look at them at 10 Pa vaccum
(whatever "semi-hydrated state means ;-)).

I would be grateful if you cared to share your opinion
on this subjet with me.

Stephane



________________________________________________________________________
____________
Bored stiff? Loosen up...
Download and play hundreds of games for free on Yahoo! Games.
http://games.yahoo.com/games/front

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From: TindallR-at-missouri.edu
Date: Tue, 16 Jan 2007 12:22:32 -0600
Subject: [Microscopy] preparation of cells for SEM - question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Stephane,

If your cells are in suspension I would coat cover slips with
poly-l-lysine (1mg/ml in distilled water), place a drop of the
suspension onto the cover slips and allow to settle for up to an hour in
a humidity chamber. Then, after the cells attach, just gently rinse the
cover slips, fix, and dehydrate using
David's HMDS protocol.

I would suggest 2% glutaraldehyde in buffer (0.1M phosphate, cacodylate,
or common buffer for biological EM) for 10-15 minutes, followed by three
or more buffer rinses, post-fixation in 1% osmium tetroxide (buffered or
aqueous) for the same time, followed by water rinses for 5-10 minutes
each, then into your dehydration schedule. Sputter coat and mount.

Good luck.

Randy

Randy Tindall
Senior EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W125 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu
On-line calendar:
http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=
Week&NavType=Both&Type=TimePlan


-----Original Message-----
X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com]
Sent: Tuesday, January 16, 2007 3:16 AM
To: Tindall, Randy D.

Dear listers,

I have a request for experts in SEM who have some experience with
eukaryotic cells.
We have a basic SEM which is normally used to study minerals, so we are
not equipped with critical point dryers and the like. Now I would like
to have a look at some cells in culture with the SEM but I have no idea
how I could prepare the cells without critical point dryer. Of course if
I simply fix and dehydrate in acetone, then air dry the cells I expect
the structure to crumble. Well perhaps it won't give nice pictures but
perhaps it will be enough for what I want to see. On the other hand
perhaps I could keep them in a semi-hydrated state and look at them at
10 Pa vaccum (whatever "semi-hydrated state means ;-)).

I would be grateful if you cared to share your opinion on this subjet
with me.

Stephane



________________________________________________________________________
____________
Bored stiff? Loosen up...
Download and play hundreds of games for free on Yahoo! Games.
http://games.yahoo.com/games/front

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From: tivol-at-caltech.edu
Date: Tue, 16 Jan 2007 18:33:28 -0600
Subject: [Microscopy] Re: preparation of cells for SEM - question

Contents Retrieved from Microscopy Listserver Archives
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On Jan 16, 2007, at 1:14 AM, nizets2-at-yahoo.com wrote:

} I have a request for experts in SEM who have some
} experience with eukaryotic cells.
} We have a basic SEM which is normally used to study
} minerals, so we are not equipped with critical point
} dryers and the like. Now I would like to have a look
} at some cells in culture with the SEM but I have no
} idea how I could prepare the cells without critical
} point dryer. Of course if I simply fix and dehydrate
} in acetone, then air dry the cells I expect the
} structure to crumble. Well perhaps it won't give nice
} pictures but perhaps it will be enough for what I want
} to see. On the other hand perhaps I could keep them in
} a semi-hydrated state and look at them at 10 Pa vaccum
} (whatever "semi-hydrated state means ;-)).
}
} I would be grateful if you cared to share your opinion
} on this subjet with me.
}
Dear Stephane,
When I was at the high-voltage TEM, I designed a hydration stage,
which allowed me to examine cells that were fully hydrated--as
determined by the fact that upon removal from the scope, at least some
of the cells were still viable. These were prepared by placing
formvar/carbon coated gold grids in the culture dish, so the cells
would grow on the grids, then just removing the grids from the dish,
placing them in a 100% humidity chamber, blotting off the excess fluid,
and transferring the grids to the hydration stage maintaining the
humidity as close to 100% as possible. If you can operate your VPSEM
at the vapor pressure of water for the temperature of the specimen
chamber (~25 torr), then you can look at them fully hydrated. I do not
know, however, whether you will see anything of interest, or just the
surface of the thin film of water that will still coat the cells. Good
luck.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu


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From: chappell.mark-at-epa.gov
Date: Tue, 16 Jan 2007 20:01:02 -0600
Subject: [Microscopy] AskAMicroscopist: SEM/EDX of bird feather

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This Question was submitted to Ask-A-Microscopist by (chappell.mark-at-epa.gov)
from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Tuesday, January 16, 2007 at 13:59:24
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Please reply to both chappell.mark-at-epa.gov as well as to the Microscopy Listserver
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Email: chappell.mark-at-epa.gov
Name: Mark Chappell

Organization: U.S. EPA

Education: Graduate College

Location: Cincinnati, OH

Title: SEM/EDX of bird feather

Question: I want to map the Hg in a contaminated bird feather using SEM/EDX/WDS. What's the best way to prepare the sample to minimize charging?

---------------------------------------------------------------------------

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From: gary-at-gaugler.com
Date: Tue, 16 Jan 2007 20:22:49 -0600
Subject: [Microscopy] Re: AskAMicroscopist: SEM/EDX of bird feather

Contents Retrieved from Microscopy Listserver Archives
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This should not be too difficult.

First look at Hg Z=80. So, high Z.
M alpha=2.191KeV. L alpha=9.986KeV.

So, for a good results you can do 5KV and look
for the Ma or do 20KV and look for Ma and La.

Coat the specimen with Pd, Ir, C, but not Au
(La=9.710KeV). Doing as suggested will or should
put Hg by itself as a EDS peak.

gary g.



At 06:02 PM 1/16/2007, you wrote:




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From: gary-at-gaugler.com
Date: Tue, 16 Jan 2007 20:27:47 -0600
Subject: [Microscopy] Re: AskAMicroscopist: SEM/EDX of bird feather

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As I think about this....this may not be possible.

The amount of Hg in the feathers are probably PPM.
EDS will not show this. You would need WDS or FTIR
to show up the small amounts....unless the feathers
are really loaded with Hg. The shape of the feathers
will also affect collection due to difflection of the
x-rays. But you should still get decent results to show
if any TRACE amounts of Hg are present.

gary g.


At 06:02 PM 1/16/2007, you wrote:




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From: hikonishi-at-gmail.com
Date: Tue, 16 Jan 2007 23:20:03 -0600
Subject: [Microscopy] BALTEK - RES-010 Benchtop Rapid Etching System

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Dear List members:

I am looking for the information on BALTEK - RES-010 Benchtop Rapid Etching
System. If you have any experience with the ion milling such as artifact and
over milling, please provide the information.

We have an old ion milling from BALTEK, but I heard that it always
overmills. Now our instrument has some problems, but no one wants to fix it
because of over milling problem. I looked at the instrument and I found that
the gun is much similar to the gun from Technoorg-Linda IV3. Based on some
experience with IV3, I think that people at our university might not use
proper way to operate it.

If you use BALTEK - RES-010, I would like to know the milling conditions
such as angle, voltage, and current. Also, I would like to know if you have
any experience of serious artifacts such as reaction, decomposition or phase
transformation.
Thank you,

Hiromi Konishi, Ph.D.
UW-Madison


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From: johnf-at-geology.wisc.edu
Date: Wed, 17 Jan 2007 07:12:43 -0600
Subject: [Microscopy] Re: SEM/EDX of bird feather

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}
}
} Question: I want to map the Hg in a contaminated bird feather using
} SEM/EDX/WDS. What's the best way to prepare the sample to minimize
} charging?

When you say "contaminated" do you mean massive contamination,
millions of times greater than what a human would get in their hair
(thru metabolism) eating fish that had "high" levels from Hg used in
adjacent gold mining/extraction? Only in that case would an
electron-based microanalytical technique be of any use.

I attempted to measure Hg in hair from native people from Brazil who
lived near gold extraction areas.The idea was to see if we could see
variations in the hair along length (thus, versus time). Using WDS
EPMA. I knew the approximate levels (thru some technique like INAA or
ICPMS or XRF, I forget) and it was high ppb or low ppm (again, I
forget the details). But I could never put enough current (I probably
went up to 10 or 20 nA) to see anything.

John


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From: frank.karl-at-degussa.com
Date: Wed, 17 Jan 2007 07:19:14 -0600
Subject: [Microscopy] Re: AskAMicroscopist: SEM/EDX of bird feather

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Wow, Some really different answers. I seem to remember Hg (2.19) falls
close to S (2.30), so I would use 25 kv because I would want to see both
the 2.19 M line and 9.8 L line. I like to be able to see at least two
lines, when possible, for any element. I would skip coating, having tried
it and always unhappy with my results and go with low pressure.

I suspect the comments about detectable level as are on target, but you
could very well surprise us.

Please let me know how this works out as I played with feathers using light
and SEM and have an interest.



chappell.mark-at-epa
.gov To: frank.karl-at-degussa.com
cc:
01/16/2007 09:02 Subject: [Microscopy] AskAMicroscopist: SEM/EDX of bird feather
PM
Please respond to
chappell.mark








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Email: chappell.mark-at-epa.gov
Name: Mark Chappell

Organization: U.S. EPA

Education: Graduate College

Location: Cincinnati, OH

Title: SEM/EDX of bird feather

Question: I want to map the Hg in a contaminated bird feather using
SEM/EDX/WDS. What's the best way to prepare the sample to minimize
charging?

---------------------------------------------------------------------------

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From: dljones-at-bestweb.net
Date: Wed, 17 Jan 2007 08:01:51 -0600
Subject: [Microscopy] looking for parts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

I apologise for taking space for this request, but I have looked many
places without success. Perhaps someone here may be able to give me some
pointers.

I'm looking for a keyboard for a SEM: a Zeiss DSM 9XX A. It could be from
a 940, 950, or 960 as I believe these are all the same keyboards. This is
for a SEM that is being used in my local high school so I'm looking for a
used, functioning keyboard that is not expensive.

If anyone knows where I might find this, please contact me. As I imagine
this is of no interest to the group, please respond to me directly.

I'm located in Cold Spring, New York USA.

Thank you,

dj


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From: TindallR-at-missouri.edu
Date: Wed, 17 Jan 2007 09:39:47 -0600
Subject: [Microscopy] Polaron E5100 sputter coater question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listers,

While our main sputter coater is out for a much-needed overhaul, we are
relying on a backup coater, namely a Polaron E5100. The unit is
operating beautifully, except that the voltage dial has worked loose at
some point and I can no longer tell where the zero point, and thus the
proper operating voltage, is located on the scale. This is because the
scale starts at 2.0, not zero, and I have no reference to line up the
indicator with. I tried looking at the set-screw scar on the knob shaft,
but it didn't help. Am I missing something obvious? (Wouldn't be the
first time.....)

f you will go to http://www.emc.missouri.edu/lookatthis.htm to see the
images of this coater hopefully this will make sense.

Can someone with a similar unit kindly let me know about where the zero
point would be on this machine? I can get it to coat by trial and
error, but it would be nice to have a baseline to experiment from.

Many thanks!

Searching for zero,
Randy


Randy Tindall
Senior EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W125 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu
On-line calendar:
http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=
Week&NavType=Both&Type=TimePlan


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From: fortner-at-cmt.anl.gov
Date: Wed, 17 Jan 2007 09:58:29 -0600
Subject: [Microscopy] AskAMicroscopist: SEM/EDX of bird feather

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

There may be a better way to do this- nondestructively- using x-rays
from the Advanced Photon Source Users' Facility at Argonne National
Laboratory. See this analysis of lead in human hair (a rather famous,
human, at that):

http://www.aps.anl.gov//News/APS_News/2000/20001017.htm

And

http://www.anl.gov/Media_Center/Frontiers/2002/c3facil.html

If you are interested, I can put you in contact with the investigators
on this project.

Jeffrey A. Fortner, Ph.D.
Chemical Engineering Division
Argonne National Laboratory
Argonne, IL 60439
fortner(at)cmt.anl.gov


.
-----Original Message-----
X-from: chappell.mark-at-epa.gov [mailto:chappell.mark-at-epa.gov]
Sent: Tuesday, January 16, 2007 8:02 PM
To: Fortner, Jeffrey A.

This Question was submitted to Ask-A-Microscopist by
(chappell.mark-at-epa.gov) from
http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on
Tuesday, January 16, 2007 at 13:59:24 Remember to consider the Grade/Age
of the student when considering the Question
------------------------------------------------------------------------
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Please reply to both chappell.mark-at-epa.gov as well as to the
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Email: chappell.mark-at-epa.gov
Name: Mark Chappell

Organization: U.S. EPA

Education: Graduate College

Location: Cincinnati, OH

Title: SEM/EDX of bird feather

Question: I want to map the Hg in a contaminated bird feather using
SEM/EDX/WDS. What's the best way to prepare the sample to minimize
charging?

------------------------------------------------------------------------
---

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From: ph2-at-sprynet.com
Date: Wed, 17 Jan 2007 10:03:46 -0600
Subject: [Microscopy] Re: SEM/EDX of bird feather

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Work on elemental composition in hair was done back in the 70s by Dr. Walter
McCrone using neutron activation. This was used to map elements by
concentration along the shaft. The basis was to look at hair
differentiation in forensics.

I recall Walter mentioning it in a short presentation at Inter-Micro ca.
2000? as well as in a conversation I had with him. I believe he also
published a piece in the The Microscope.

Tony


......................................................................
Andrew Anthony "Tony" Havics, CHMM, CIH, PE
pH2, LLC
5250 E US 36, Suite 830
Avon, IN 46123
(317) 718-7020 off
(317) 718-7038 fax
(317) 409-3238 cell

90% of Risk Management is knowing where to place the decimal point...any
consultant can give you the other 10%(SM)

This message is from pH2. This message and any attachments may contain
legally privileged or confidential information, and are intended only for
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distributed without this statement.


-----Original Message-----
X-from: johnf-at-geology.wisc.edu [mailto:johnf-at-geology.wisc.edu]
Sent: Wednesday, January 17, 2007 8:22 AM
To: ph2-at-sprynet.com

}
}
} Question: I want to map the Hg in a contaminated bird feather using
} SEM/EDX/WDS. What's the best way to prepare the sample to minimize
} charging?

When you say "contaminated" do you mean massive contamination,
millions of times greater than what a human would get in their hair
(thru metabolism) eating fish that had "high" levels from Hg used in
adjacent gold mining/extraction? Only in that case would an
electron-based microanalytical technique be of any use.

I attempted to measure Hg in hair from native people from Brazil who
lived near gold extraction areas.The idea was to see if we could see
variations in the hair along length (thus, versus time). Using WDS
EPMA. I knew the approximate levels (thru some technique like INAA or
ICPMS or XRF, I forget) and it was high ppb or low ppm (again, I
forget the details). But I could never put enough current (I probably
went up to 10 or 20 nA) to see anything.

John


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From: ph2-at-sprynet.com
Date: Wed, 17 Jan 2007 10:19:49 -0600
Subject: [Microscopy] Re: SEM/EDX of bird feather

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

It got the better of me:

The article was:

McCrone, Walter, and Michael Bayard: Individualization of Hair. Microscope
47(3):129-133. 1999.

Good article. Ion microprobe.

Walter passed away a few years ago, but Mike Bayard is still around (Bayard
consulting).

Tony

......................................................................
Andrew Anthony "Tony" Havics, CHMM, CIH, PE
pH2, LLC
5250 E US 36, Suite 830
Avon, IN 46123
(317) 718-7020 off
(317) 718-7038 fax
(317) 409-3238 cell

90% of Risk Management is knowing where to place the decimal point...any
consultant can give you the other 10%(SM)

This message is from pH2. This message and any attachments may contain
legally privileged or confidential information, and are intended only for
the individual or entity identified above as the addressee. If you are not
the addressee, or if this message has been addressed to you in error, you
are not authorized to read, copy, or distribute this message and any
attachments, and we ask that you please delete this message and attachments
(including all copies) and notify the sender by return e-mail or by phone at
317-718-7020. Delivery of this message and any attachments to any person
other than the intended recipient(s) is not intended in any way to waive
confidentiality or a privilege. All personal messages express views only of
the sender, which are not to be attributed to pH2 and may not be copied or
distributed without this statement.


-----Original Message-----
X-from: johnf-at-geology.wisc.edu [mailto:johnf-at-geology.wisc.edu]
Sent: Wednesday, January 17, 2007 8:22 AM
To: ph2-at-sprynet.com

}
}
} Question: I want to map the Hg in a contaminated bird feather using
} SEM/EDX/WDS. What's the best way to prepare the sample to minimize
} charging?

When you say "contaminated" do you mean massive contamination,
millions of times greater than what a human would get in their hair
(thru metabolism) eating fish that had "high" levels from Hg used in
adjacent gold mining/extraction? Only in that case would an
electron-based microanalytical technique be of any use.

I attempted to measure Hg in hair from native people from Brazil who
lived near gold extraction areas.The idea was to see if we could see
variations in the hair along length (thus, versus time). Using WDS
EPMA. I knew the approximate levels (thru some technique like INAA or
ICPMS or XRF, I forget) and it was high ppb or low ppm (again, I
forget the details). But I could never put enough current (I probably
went up to 10 or 20 nA) to see anything.

John


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From: mike.wombwell-at-quorumtech.com
Date: Wed, 17 Jan 2007 10:21:14 -0600
Subject: [Microscopy] Polaron E5100 sputter coater question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear Randy,

The maximum voltage from the HV transformer is 2800 volts so it would be
safe to set the control knob shaft to the fully clockwise position and
place the knob at the 2.8 kV end of the scale.
For reference, this is a very old coater (discontinued in around 1988)
but some parts are still available. For further support please contact
our US distributor Energy Beam Sciences
(http://www.quorumtech.com/contact_us/usa.htm).

You can also find a download of the E5100 manual on the technical
support pages of our website
(http://www.quorumtech.com/Tech_Support/polaron-range-technical-support.
htm)

I hope this helps.

Best regards,

Mike Wombwell
Quorum Technologies
Newhaven, East Sussex, UK
Tel: +44(0)1273 510535
Fax: +44(0)1273 510536
mike.wombwell-at-quorumtech.com
http://www.quorumtech.com

E & O E


-----Original Message-----
X-from: TindallR-at-missouri.edu [mailto:TindallR-at-missouri.edu]
Sent: 17 January 2007 15:44
To: Mike Wombwell

Dear Listers,

While our main sputter coater is out for a much-needed overhaul, we are
relying on a backup coater, namely a Polaron E5100. The unit is
operating beautifully, except that the voltage dial has worked loose at
some point and I can no longer tell where the zero point, and thus the
proper operating voltage, is located on the scale. This is because the
scale starts at 2.0, not zero, and I have no reference to line up the
indicator with. I tried looking at the set-screw scar on the knob shaft,
but it didn't help. Am I missing something obvious? (Wouldn't be the
first time.....)

f you will go to http://www.emc.missouri.edu/lookatthis.htm to see the
images of this coater hopefully this will make sense.

Can someone with a similar unit kindly let me know about where the zero
point would be on this machine? I can get it to coat by trial and
error, but it would be nice to have a baseline to experiment from.

Many thanks!

Searching for zero,
Randy


Randy Tindall
Senior EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W125 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu
On-line calendar:
http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=
Week&NavType=Both&Type=TimePlan


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From: eggert-at-mikroanalytik.de
Date: Wed, 17 Jan 2007 10:22:16 -0600
Subject: [Microscopy] Re: FW: AskAMicroscopist: SEM/EDX of bird feather

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Mark,

I would also try to use laboratory micro-XRF before using a synchrotron
source. The advantages against common SEM excitation are much better
detection limits (because there is no bremsstrahlung in spectra
background) and no need of vacuum. I think, micro-XRF meets the needs of
your analysis goals.

Frank Eggert

===============================
http://www.microanalyst.net
===============================


fortner-at-cmt.anl.gov wrote:

} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


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From: TindallR-at-missouri.edu
Date: Wed, 17 Jan 2007 10:44:54 -0600
Subject: [Microscopy] Polaron E5100 sputter coater question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Mike,

Your link didn't work, but I was able to get to the manual with
http://www.quorumtech.com/Manuals/E5100-manual.pdf. Thanks very much
for this. I'm posting this to the listserve, in case others have this
problem also.

I think we've got the problem solved with your suggestion of setting the
maximum setting at 2.8 on the dial. Full counterclockwise with this pot
then comes out at about 3:00, as Richard Kucklinski also noted in a
off-list posting to me. This makes sense now.

I'm suspecting an argon leak, since with the argon turned fully OFF, I'm
still getting } 40 mA coating current with the dial set at 2.0-2.2. I'll
experiment around with it to get it fine-tuned a bit better, but it's
coating reliably now.

Many thanks to all who emailed and phoned. What a resource!!

Cheers,
Randy

-----Original Message-----
X-from: mike.wombwell-at-quorumtech.com [mailto:mike.wombwell-at-quorumtech.com]

Sent: Wednesday, January 17, 2007 10:22 AM
To: Tindall, Randy D.

Dear Randy,

The maximum voltage from the HV transformer is 2800 volts so it would be
safe to set the control knob shaft to the fully clockwise position and
place the knob at the 2.8 kV end of the scale.
For reference, this is a very old coater (discontinued in around 1988)
but some parts are still available. For further support please contact
our US distributor Energy Beam Sciences
(http://www.quorumtech.com/contact_us/usa.htm).

You can also find a download of the E5100 manual on the technical
support pages of our website
(http://www.quorumtech.com/Tech_Support/polaron-range-technical-support.
htm)

I hope this helps.

Best regards,

Mike Wombwell
Quorum Technologies
Newhaven, East Sussex, UK
Tel: +44(0)1273 510535
Fax: +44(0)1273 510536
mike.wombwell-at-quorumtech.com
http://www.quorumtech.com

E & O E


-----Original Message-----
X-from: TindallR-at-missouri.edu [mailto:TindallR-at-missouri.edu]
Sent: 17 January 2007 15:44
To: Mike Wombwell

Dear Listers,

While our main sputter coater is out for a much-needed overhaul, we are
relying on a backup coater, namely a Polaron E5100. The unit is
operating beautifully, except that the voltage dial has worked loose at
some point and I can no longer tell where the zero point, and thus the
proper operating voltage, is located on the scale. This is because the
scale starts at 2.0, not zero, and I have no reference to line up the
indicator with. I tried looking at the set-screw scar on the knob shaft,
but it didn't help. Am I missing something obvious? (Wouldn't be the
first time.....)

f you will go to http://www.emc.missouri.edu/lookatthis.htm to see the
images of this coater hopefully this will make sense.

Can someone with a similar unit kindly let me know about where the zero
point would be on this machine? I can get it to coat by trial and
error, but it would be nice to have a baseline to experiment from.

Many thanks!

Searching for zero,
Randy


Randy Tindall
Senior EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W125 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu
On-line calendar:
http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=
Week&NavType=Both&Type=TimePlan


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From: mager-at-interchange.ubc.ca
Date: Wed, 17 Jan 2007 11:09:31 -0600
Subject: [Microscopy] AskAMicroscopist: SEM/EDX of bird feather

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Dear Mark,
I have done this in the past, for the Royal Museum in Victoria, BC. They
were interested in the contamination of feathers and fur in old stuffed
animals and birds, because mercury and arsenic used to be used to preserve
museum samples many years ago and the museum wanted to know if these
exhibits were contaminated with heavy elements before they were put on
display or exposed them to the public. The heavy elements were expected to
be applied to the feathers, so the amounts were large, if they were there. I
used the SEM in variable pressure mode, because fluffy things are so
difficult to coat effectively, and just scanned around with the
back-scattered detector, looking for bright things. Any I found I took a
picture of and analysed with EDX. I have also detected elevated levels of
copper in someone's hair using SEM+EDX.
Looking for smaller amounts of heavy elements or organically-bound material
in the interior of the sample would require the sample to be mounted in
epoxy, polished, carbon-coated and then careful WDX analysis.
Good luck,
Mary Mager
Electron Microscopist
Materials Eng. UBC
#419 - 6350 Stores Rd.
Vancouver, B.C. V6T 1Z4
CANADA
Phone: 604-822-5648
Fax: 604-822-3619
email: mager-at-interchange.ubc.ca

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Email: chappell.mark-at-epa.gov
Name: Mark Chappell

Organization: U.S. EPA

Education: Graduate College

Location: Cincinnati, OH

Title: SEM/EDX of bird feather

Question: I want to map the Hg in a contaminated bird feather using
SEM/EDX/WDS. What's the best way to prepare the sample to minimize
charging?

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From: eschumacher-at-mccrone.com
Date: Wed, 17 Jan 2007 11:22:34 -0600
Subject: [Microscopy] AskAMicroscopist: SEM/EDX of bird feather

Contents Retrieved from Microscopy Listserver Archives
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Posted on behalf of a colleague here at McCrone:

Actually, the work done by Dr. McCrone in the early 1970's on a forensic
hair case from Detroit, used an ion microprobe/secondary ion mass
spectrometry (SIMS) rather than neutron activation analysis (NAA). We
have abandoned the idea of mapping the trace elemental concentrations
along the shaft of the hair due to many difficulties with that approach.
More recently we concentrated trace elements in hairs by low temperature
ashing (LTA) and used SIMS to identify and measure trace element
concentrations in the ash. The quantification isn't very accurate and
the levels need to be compared with good positive and negative controls
because there is no baseline data for comparison. In other words, you
might determine that a questioned hair contains levels of lead, for
example, greater than expected in hairs from normal people where they
have not received significant exposure to lead. We presume the same
approach could be used for feathers. Of course, the other option is to
use a bulk method such as XRF at Argonne. For more information contact
Dick Bisbing off-line at dbisbing-at-mccrone.com.

Regards,

Elaine
*********************************************************************
Elaine F.Schumacher
Senior Research Scientist
McCrone Associates, Inc.
850 Pasquinelli Drive
Westmont, IL 60559-5539 USA
630-887-7100 (tel)
630-887-7417 (fax)
E-mail: eschumacher-at-mccrone.com
Web Site: www.mccrone.com



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intended recipient. If you are not the intended recipient,
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-----Original Message-----
X-from: chappell.mark-at-epa.gov [mailto:chappell.mark-at-epa.gov]
Sent: Tuesday, January 16, 2007 8:06 PM
To: Elaine F. Schumacher

This Question was submitted to Ask-A-Microscopist by
(chappell.mark-at-epa.gov)
from
http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on
Tuesday, January 16, 2007 at 13:59:24
Remember to consider the Grade/Age of the student when considering the
Question
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Email: chappell.mark-at-epa.gov
Name: Mark Chappell

Organization: U.S. EPA

Education: Graduate College

Location: Cincinnati, OH

Title: SEM/EDX of bird feather

Question: I want to map the Hg in a contaminated bird feather using
SEM/EDX/WDS. What's the best way to prepare the sample to minimize
charging?

------------------------------------------------------------------------
---

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From: sue.tyler-at-noaa.gov
Date: Wed, 17 Jan 2007 18:33:45 -0600
Subject: [Microscopy] viaWWW: TEM and ecological assesment

Contents Retrieved from Microscopy Listserver Archives
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Hi Randy

If I remember correctly the top end of the E5000 series variac scale was
2.8kV. Best results, if you do not want to cook your specimen, are achieved
at 20mA and around 800 volts in other words about 1/3 to 1/2 scale from
minimum.

Hope this helps?

Steve Chapman
Protrain for EM training & consultancy world wide
www.emcourses.com
Tel +44 1280 816512 Fax +44 1280 814007
Mobile +44 7711 606967

----- Original Message -----
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To: {protrain-at-emcourses.com}
Sent: Wednesday, January 17, 2007 4:45 PM

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Email: sue.tyler-at-noaa.gov
Name: Sue Tyler

Organization: Cooperative Oxford Laboratory

Title-Subject: [Filtered] TEM and ecological assesment

Question: My laboratory is changing directions and shifting from disease research to ecosystem health assessment. The past users of the TEM lab have asked me to justify the need for TEM in this new line of work. I am the technician and not aware of current research in the field of ecosystem health. I have received a few papers off the internet. There are a few institutions who use TEM in that field, but I was wondering if there are anymore out there? Can you point me in the right direction?

With the wealth of knowlege in "microscopy land" I am sure someone can help me.

Thanks in advance

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From: klangwor-at-uoregon.edu
Date: Wed, 17 Jan 2007 18:34:23 -0600
Subject: [Microscopy] viaWWW: EBL/ PMMA Mask

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Email: klangwor-at-uoregon.edu
Name: Kurt Langworthy

Organization: University of Oregon

Title-Subject: [Filtered] EBL/ PMMA Mask

Question: All,
I'm looking for a gold etching recipt that will allow me to use PMMA as a mask. KCN looks promising, but is highly toxic. Does anyone have any better ideas?
Thanks,
Kurt

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From: TindallR-at-missouri.edu
Date: Thu, 18 Jan 2007 10:12:20 -0600
Subject: [Microscopy] Spark emission test?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

And along a completely different line, we've been asked if we can do a
"spark emission test". Since I don't know what that is, and Google
hasn't helped, I don't know if we can or not.

Has anybody heard of this test? Seems to be a way of checking sample
composition at concentrations below those attainable by EDS, but that's
about all I can tell.

Sorry if this isn't microscopy-related, but I don't even know that, at
this point. We'll see what the collective comes up with on this one!

Thanks to all.

Randy

Randy Tindall
Senior EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W125 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu
On-line calendar:
http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=
Week&NavType=Both&Type=TimePlan


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From: NWWhite-at-bwxt.com
Date: Thu, 18 Jan 2007 10:24:47 -0600
Subject: [Microscopy] Spark emission test?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Randy,

I don't have one, but am familiar with the technique. It is not
uncommon in foundry and other metallurgical areas. The instrument
generates an electric arc to a (typically metallic) specimen and "looks"
at the resulting emission spectrum. It is not useful for microanalysis,
but the larger analysis volume works pretty well for average
compositions. I don't remember the detection limit(s), but it is pretty
low. Another advantage is that it is quick and there is little sample
preparation required.

Hope this brief response is helpful...
Woody White
BWXT Services



-----Original Message-----
X-from: TindallR-at-missouri.edu [mailto:TindallR-at-missouri.edu]
Sent: Thursday, January 18, 2007 11:13 AM
To: White, Woody N.

And along a completely different line, we've been asked if we can do a
"spark emission test". Since I don't know what that is, and Google
hasn't helped, I don't know if we can or not.

Has anybody heard of this test? Seems to be a way of checking sample
composition at concentrations below those attainable by EDS, but that's
about all I can tell.

Sorry if this isn't microscopy-related, but I don't even know that, at
this point. We'll see what the collective comes up with on this one!

Thanks to all.

Randy

Randy Tindall
Senior EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W125 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu
On-line calendar:
http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=
Week&NavType=Both&Type=TimePlan


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From: frank.karl-at-degussa.com
Date: Thu, 18 Jan 2007 10:33:13 -0600
Subject: [Microscopy] Re: Spark emission test?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Randy,
While this could be some type of laser emission spectroscopy test, I have
also read about spark test for metals. As I remember it different steels
and other metals will shoot out different colored, shaped, morphology ,
behavior (you catch the drift ?) sparks when touch to a grind wheel. It's
been reported that an experienced operator can tell what grade of stainless
steel based on the properties of sparks.

Never tried this myself.......... Too hard to get the grinding wheel under
my stereomicroscope......




TindallR-at-missouri
.edu To: frank.karl-at-degussa.com
cc:
01/18/2007 11:14 Subject: [Microscopy] Spark emission test?
AM
Please respond to
TindallR








----------------------------------------------------------------------------

The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


And along a completely different line, we've been asked if we can do a
"spark emission test". Since I don't know what that is, and Google
hasn't helped, I don't know if we can or not.

Has anybody heard of this test? Seems to be a way of checking sample
composition at concentrations below those attainable by EDS, but that's
about all I can tell.

Sorry if this isn't microscopy-related, but I don't even know that, at
this point. We'll see what the collective comes up with on this one!

Thanks to all.

Randy

Randy Tindall
Senior EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W125 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu
On-line calendar:
http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=
Week&NavType=Both&Type=TimePlan


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From: dljones-at-bestweb.net
Date: Thu, 18 Jan 2007 11:15:32 -0600
Subject: [Microscopy] Re: Spark emission test?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Randy,

Your question of "spark emission test" sounds familiar but I can't put my
finger on it. I can think of three techniques that may be what is being
asked: Spark Testing, Spark Source Mass Spectrometery, or Optical Emission
Spectroscopy.

Spark Testing - this is a technique that simply uses a grinder. A piece of
metal is pushed against the grinder. The sparks that fly off are
indicative of what the alloy is. I only know of it being used in metal
alloys. It would be the kind of test someone dealing with a scrap yard
would find useful. It can basically tell you what grade of metal you are
dealing with. It takes a fair amount of practice to be able to visually
know what alloy the spark pattern is indicating. People that do this test
frequently can do quite well with it as far as getting a good handle on
what alloy they have in their hand.

Spark Source Mass Spectrometery - This is used for a wide range of
samples, but tends to do better with samples that are conductive, although
that is not necessarily a limitation. There are two other techniques that
are related to this: Laser Ionization Mass Spectrometery and Inductively
Coupled Plasma Atomic Emission.

Optical Emission Spectroscopy - I'm not too familiar with this technique,
but I seem to recall they will often use a spark source in the process.
When they do that, I think this is then called Spark Emission
Spectroscopy. You might want to check out Bruker AXS Microanalysis, I
believe they make some of these instruments.

Perhaps if you post what they are testing and what they want from the
analysis, that may help. I hope I've given you at least some food for
thought.

Good luck,

dj



On Thu, 18 Jan 2007, TindallR-at-missouri.edu wrote:

}
}
}
} ----------------------------------------------------------------------------
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}
} And along a completely different line, we've been asked if we can do a
} "spark emission test". Since I don't know what that is, and Google
} hasn't helped, I don't know if we can or not.
}
} Has anybody heard of this test? Seems to be a way of checking sample
} composition at concentrations below those attainable by EDS, but that's
} about all I can tell.
}
} Sorry if this isn't microscopy-related, but I don't even know that, at
} this point. We'll see what the collective comes up with on this one!
}
} Thanks to all.
}
} Randy
}
} Randy Tindall
} Senior EM Specialist
} Electron Microscopy Core Facility---We Do Small Well!
} W125 Veterinary Medicine
} University of Missouri
} Columbia, MO 65211
} Tel: (573) 882-8304
} Fax: (573) 884-2227
} Email: tindallr-at-missouri.edu
} Web: http://www.emc.missouri.edu
} On-line calendar:
} http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=
} Week&NavType=Both&Type=TimePlan
}
}
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From: werner-at-rosharon.oilfield.slb.com
Date: Thu, 18 Jan 2007 13:55:03 -0600
Subject: [Microscopy] Spark emission test?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

dj is right, when metallurgists say "spark test" they generally mean
optical emission spectroscopy (OES). OES can give reliable sulfur and
phosphorous (for instance) readings at low concentrations I would never
have the chutzpa to report from EDS.

The grinding wheel test can distinguish high-carbon (short sparks, in dense
clusters) from low-carbon steels (few sparks, long, and not in
clusters). I do not think it can distinguish whether any other alloying
elements are present or not. There is a great ASTM document - STP 550
"Nondestructive Rapid Identification of Metals and Alloys by Spot Test"
from 1974 that covers chemical spot tests for qualitative alloy i.d. but
that is 'way off-topic.

Regards,
Andrew

At 11:16 AM 1/18/2007, you wrote:



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From: bigelow-at-engin.umich.edu
Date: Thu, 18 Jan 2007 13:59:24 -0600
Subject: [Microscopy] RE: Etching gold

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

In an ancient edition of The Metals Handbook (Amer Soc for Materials,
ASM) I found the following :


"The most widely used nonelectrolytic etch is a mixture of equal
volumes of 10% ammonium persulfate in water with 10% KCN in water.
Although the separate solutions are stable in air, the mixture must
be used within a few minutes of mixing. Since this solution and the
fumes from it are poisonous, it should be used under a hood (and
with other relevant precautions) . . . . . . When swabbed on
specimens of the usual gold alloys and palladium alloys the mixture
acts rapidly and smoothly. It may also be used on silver and certain
nickle alloys. For more resistant alloys, 20% solutions may be
employed"

"The same metals can be etched when made the anode in a 5% KCN
solution" (I'd suggest a voltage of about 5 V). Also mentioned is
electrolytic etching using an a.c. voltage (you can probably use a
variac as a source for this, again about 5 volts) with a 20% solution
of hydrochloric acid saturated with sodium chloride.

The great advantage of Gold and the other noble metals is their
chemical stabilite, and so your task is a non-trivial one.

Good luck,
--
Wilbur C. Bigelow, Professor Emeritus
Materials Sci. & Engr., Univ. of Michigan
Ann Arbor, Michigan 48109-2136
e-mail: bigelow-at-engin.umich.edu;
Fx:734-763-4788; Ph:734-975-0858
Address mail to: 2911 Whittier Court
Ann Arbor, MI 48104-6731

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From: smalinskas-at-yahoo.com
Date: Thu, 18 Jan 2007 14:22:27 -0600
Subject: [Microscopy] Spark emission test?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Randy,

This sounds like optical emission arc-spectrometry.
It sparks a metal sample and the different light
wavelengths are channeled and read to quantify the
elements. We use it in our lab for accurate
measurements of elemental content in different metal
alloys. This technique is considered very accurate,
considerably better and more reliable than EDS
techniques. Some elements can be detected in the ppm
range.

Stu Smalinskas, P.E.
Metallurgist
SKF
Plymouth, Michigan
(734) 414-6862

--- frank.karl-at-degussa.com wrote:

} Randy,
} While this could be some type of laser emission
} spectroscopy test, I have
} also read about spark test for metals. As I
} remember it different steels
} and other metals will shoot out different colored,
} shaped, morphology ,
} behavior (you catch the drift ?) sparks when touch
} to a grind wheel. It's
} been reported that an experienced operator can tell
} what grade of stainless
} steel based on the properties of sparks.
}
} Never tried this myself.......... Too hard to get
} the grinding wheel under
} my stereomicroscope......
}
}
}
}
}
--- Randy wrote:
}
} And along a completely different line, we've been
} asked if we can do a
} "spark emission test". Since I don't know what that
} is, and Google
} hasn't helped, I don't know if we can or not.
}
} Has anybody heard of this test? Seems to be a way
} of checking sample
} composition at concentrations below those attainable
} by EDS, but that's
} about all I can tell.
}
} Sorry if this isn't microscopy-related, but I don't
} even know that, at
} this point. We'll see what the collective comes up
} with on this one!
}
} Thanks to all.
}
} Randy
}
} Randy Tindall
} Senior EM Specialist
} Electron Microscopy Core Facility---We Do Small
} Well!
} W125 Veterinary Medicine
} University of Missouri
} Columbia, MO 65211
} Tel: (573) 882-8304
} Fax: (573) 884-2227
} Email: tindallr-at-missouri.edu
} Web: http://www.emc.missouri.edu
} On-line calendar:
}
http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=
} Week&NavType=Both&Type=TimePlan
}



____________________________________________________________________________________
Do you Yahoo!?
Everyone is raving about the all-new Yahoo! Mail beta.
http://new.mail.yahoo.com

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From: TindallR-at-missouri.edu
Date: Thu, 18 Jan 2007 14:34:50 -0600
Subject: [Microscopy] Spark emission test

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Many thanks to all who responded to my query about spark emission
testing. I have passed on the information to the client who requested
it, and even found a local source to do the test----right here in the UM
system.

Sorry for cluttering up the list with what turned out to be a
non-microscopy issue, but I learned something new.

Next question: is there anything the listserve denizens can't answer?

Thanks again!

Randy

Randy Tindall
Senior EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W125 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu
On-line calendar:
http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=
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From: dljones-at-bestweb.net
Date: Thu, 18 Jan 2007 15:57:08 -0600
Subject: [Microscopy] Re: Spark emission test?

Contents Retrieved from Microscopy Listserver Archives
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Andrew,

Actually, the grinding wheel test can tell a lot more than one would
think. You can tell stainless steels, high speed steels, if the alloy
contains cobalt, and more. I used to have an old text that listed the
spark patterns for a wide range of alloys. But I haven't been able to find
it in years. It was handed out in a technical course on welding I took
many years ago at a community college. This was an excellent reference
that had several pages of spark patterns in color identifying the various
alloys that each spark test indicated. It has to be in color as color is
one of the identifiers. If anyone knows of a reference that has an
extensive color chart like this, can you please send me the reference?
I've been looking for a long time to find this again.

Randy,

You didn't say what the specific test was you have found to be it. Is it
indeed OES?

dj


On Thu, 18 Jan 2007, werner-at-rosharon.oilfield.slb.com wrote:

} Date: Thu, 18 Jan 2007 13:59:42 -0600
} From: werner-at-rosharon.oilfield.slb.com
} To: dljones-at-bestweb.net
} Subject: [Microscopy] Spark emission test?
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} dj is right, when metallurgists say "spark test" they generally mean
} optical emission spectroscopy (OES). OES can give reliable sulfur and
} phosphorous (for instance) readings at low concentrations I would never
} have the chutzpa to report from EDS.
}
} The grinding wheel test can distinguish high-carbon (short sparks, in dense
} clusters) from low-carbon steels (few sparks, long, and not in
} clusters). I do not think it can distinguish whether any other alloying
} elements are present or not. There is a great ASTM document - STP 550
} "Nondestructive Rapid Identification of Metals and Alloys by Spot Test"
} from 1974 that covers chemical spot tests for qualitative alloy i.d. but
} that is 'way off-topic.
}
} Regards,
} Andrew
}
} At 11:16 AM 1/18/2007, you wrote:
}
}
}
} } ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
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} } ----------------------------------------------------------------------------
} }
} } Randy,
} }
} } Your question of "spark emission test" sounds familiar but I can't put my
} } finger on it. I can think of three techniques that may be what is being
} } asked: Spark Testing, Spark Source Mass Spectrometery, or Optical Emission
} } Spectroscopy.
} }
} } Spark Testing - this is a technique that simply uses a grinder. A piece of
} } metal is pushed against the grinder. The sparks that fly off are
} } indicative of what the alloy is. I only know of it being used in metal
} } alloys. It would be the kind of test someone dealing with a scrap yard
} } would find useful. It can basically tell you what grade of metal you are
} } dealing with. It takes a fair amount of practice to be able to visually
} } know what alloy the spark pattern is indicating. People that do this test
} } frequently can do quite well with it as far as getting a good handle on
} } what alloy they have in their hand.
} }
} } Spark Source Mass Spectrometery - This is used for a wide range of
} } samples, but tends to do better with samples that are conductive, although
} } that is not necessarily a limitation. There are two other techniques that
} } are related to this: Laser Ionization Mass Spectrometery and Inductively
} } Coupled Plasma Atomic Emission.
} }
} } Optical Emission Spectroscopy - I'm not too familiar with this technique,
} } but I seem to recall they will often use a spark source in the process.
} } When they do that, I think this is then called Spark Emission
} } Spectroscopy. You might want to check out Bruker AXS Microanalysis, I
} } believe they make some of these instruments.
} }
} } Perhaps if you post what they are testing and what they want from the
} } analysis, that may help. I hope I've given you at least some food for
} } thought.
} }
} } Good luck,
} }
} } dj
} }
} }
} }
} } On Thu, 18 Jan 2007, TindallR-at-missouri.edu wrote:
} }
} } }
} } }
} } }
} } }
} } ----------------------------------------------------------------------------
} } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } }
} } ----------------------------------------------------------------------------
} } }
} } } And along a completely different line, we've been asked if we can do a
} } } "spark emission test". Since I don't know what that is, and Google
} } } hasn't helped, I don't know if we can or not.
} } }
} } } Has anybody heard of this test? Seems to be a way of checking sample
} } } composition at concentrations below those attainable by EDS, but that's
} } } about all I can tell.
} } }
} } } Sorry if this isn't microscopy-related, but I don't even know that, at
} } } this point. We'll see what the collective comes up with on this one!
} } }
} } } Thanks to all.
} } }
} } } Randy
} } }
} } } Randy Tindall
} } } Senior EM Specialist
} } } Electron Microscopy Core Facility---We Do Small Well!
} } } W125 Veterinary Medicine
} } } University of Missouri
} } } Columbia, MO 65211
} } } Tel: (573) 882-8304
} } } Fax: (573) 884-2227
} } } Email: tindallr-at-missouri.edu
} } } Web: http://www.emc.missouri.edu
} } } On-line calendar:
} } } http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=
} } } Week&NavType=Both&Type=TimePlan
} } }
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From: gregm-at-andersonlabs.com
Date: Thu, 18 Jan 2007 17:54:18 -0600
Subject: [Microscopy] viaWWW: Spark Spectroscopy

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Email: gregm-at-andersonlabs.com
Name: Greg Mann

Title-Subject: [Filtered] Spark Spectroscopy

Question: Optical Emission Spectroscopy (OES) commonly uses an AC spark source to excite a small volume of metallic sample in a coronal discharge. In one application a high frequency AC source strikes an arc with a tungsten electrode. Basically the excited electrons have jumped an electron shell, and on return to their relaxed state, they emit a photon of light characteristic of their source. The light generated is directed into an evacuated spectral box, passed through a grating and separated into its spectral components. Each wavelength of interest (i.e. element of interest) is detected. Solid state detectors have can be nearly continuous in wavelength detection, while traditional photo multipliers are discreet in location, often with greater sensitivity. Other OES techniques may employ a DC arc, glow discharge source, or Inductively coupled plasma (ICP). Not to be confused the older generation spark station software (I believe it was Leica Cambridge). Or the grinding wheel test that looks at the color of the spark, and the lengths of the fingers on the generated spark to estimate manganese and carbon contents.

For more information, contact me offline, as we do quite a bit of these bulk analysis metals.

Greg M



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From: dainis-at-red5wood.com
Date: Thu, 18 Jan 2007 17:54:46 -0600
Subject: [Microscopy] viaWWW: Leica 1400 microtome manual etc

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Email: dainis-at-red5wood.com
Name: Dainis

Title-Subject: [Filtered] Leica 1400 microtome manual etc

Question: Dear Microscopists
Does anyone have a spare (or is prepared to copy) manual for the Leica 1400 microtome please?
Also has anyone used ImageJ with the CRI RGB Micro Color filter?
Thanks
Dainis

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From: mjaffer-at-science.uct.ac.za
Date: Fri, 19 Jan 2007 01:21:29 -0600
Subject: [Microscopy] Disposal of JEOL 200CX

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Dear All

We need to dispose our working 1979 vintage JEOL 200CX to make way for
a new instrument. The microscope will be taken out of service by of
February 2007. The complete instrument or parts will be available to
anyone who is interested. We have the manuals, service diagrams, a
variety of holders as well as a few spare parts for this instrument.
Please contact me offline if you are interested.

Thanks in advance.

Mohamed

************************************
Mohamed Jaffer
Electron Microscope Unit
University of Cape Town
Private Bag
Rondebosch, 7701
South Africa

Tel: +27 21 6503354
Fax: + 27 21 6891528

Email: mjaffer-at-science.uct.ac.za

************************************






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From: nmedvitz-at-bostwicklaboratories.com
Date: Fri, 19 Jan 2007 08:47:04 -0600
Subject: [Microscopy] viaWWW: TEM job opening

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Email: nmedvitz-at-bostwicklaboratories.com
Name: Neil Medvitz

Organization: Bostwick Laboratories

Title-Subject: [Filtered] TEM job opening

Question: I am looking to hire a couple people to do EM in a renal pathology lab in the Richmond Virginia area. Please contact mr via e-mail if interested.

Neil Medvitz
Electron Microscopist
Bostwick Laboratoriesô
For Absolute ConfidenceÆ

4355 Innslake Drive
Glen Allen, Virginia 23060
Phone: (804) 967-9225 x 1488
Toll Free: (800) 214-6628
Email: nmedvitz-at-bostwicklaboratories.com




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From: fschamber-at-aspexcorp.com
Date: Fri, 19 Jan 2007 10:36:01 -0600
Subject: [Microscopy] Re: Spark emission test?

Contents Retrieved from Microscopy Listserver Archives
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-----Original Message-----
X-from: dljones-at-bestweb.net [mailto:dljones-at-bestweb.net]
Sent: Thursday, January 18, 2007 5:05 PM
To: Fred Schamber

Andrew,

Actually, the grinding wheel test can tell a lot more than one would
think. You can tell stainless steels, high speed steels, if the alloy
contains cobalt, and more. I used to have an old text that listed the
spark patterns for a wide range of alloys. But I haven't been able to
find
it in years. It was handed out in a technical course on welding I took
many years ago at a community college. This was an excellent reference
that had several pages of spark patterns in color identifying the
various
alloys that each spark test indicated. It has to be in color as color is

one of the identifiers. If anyone knows of a reference that has an
extensive color chart like this, can you please send me the reference?
I've been looking for a long time to find this again.

Randy,

You didn't say what the specific test was you have found to be it. Is it

indeed OES?

dj


On Thu, 18 Jan 2007, werner-at-rosharon.oilfield.slb.com wrote:

} Date: Thu, 18 Jan 2007 13:59:42 -0600
} From: werner-at-rosharon.oilfield.slb.com
} To: dljones-at-bestweb.net
} Subject: [Microscopy] Spark emission test?
}
}
}
}
}
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----
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
America
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}
} dj is right, when metallurgists say "spark test" they generally mean
} optical emission spectroscopy (OES). OES can give reliable sulfur and
} phosphorous (for instance) readings at low concentrations I would
never
} have the chutzpa to report from EDS.
}
} The grinding wheel test can distinguish high-carbon (short sparks, in
dense
} clusters) from low-carbon steels (few sparks, long, and not in
} clusters). I do not think it can distinguish whether any other
alloying
} elements are present or not. There is a great ASTM document - STP 550
} "Nondestructive Rapid Identification of Metals and Alloys by Spot
Test"
} from 1974 that covers chemical spot tests for qualitative alloy i.d.
but
} that is 'way off-topic.
}
} Regards,
} Andrew
}
} At 11:16 AM 1/18/2007, you wrote:
}
}
}
} }
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} }
} } Randy,
} }
} } Your question of "spark emission test" sounds familiar but I can't
put my
} } finger on it. I can think of three techniques that may be what is
being
} } asked: Spark Testing, Spark Source Mass Spectrometery, or Optical
Emission
} } Spectroscopy.
} }
} } Spark Testing - this is a technique that simply uses a grinder. A
piece of
} } metal is pushed against the grinder. The sparks that fly off are
} } indicative of what the alloy is. I only know of it being used in
metal
} } alloys. It would be the kind of test someone dealing with a scrap
yard
} } would find useful. It can basically tell you what grade of metal you
are
} } dealing with. It takes a fair amount of practice to be able to
visually
} } know what alloy the spark pattern is indicating. People that do this
test
} } frequently can do quite well with it as far as getting a good handle
on
} } what alloy they have in their hand.
} }
} } Spark Source Mass Spectrometery - This is used for a wide range of
} } samples, but tends to do better with samples that are conductive,
although
} } that is not necessarily a limitation. There are two other techniques
that
} } are related to this: Laser Ionization Mass Spectrometery and
Inductively
} } Coupled Plasma Atomic Emission.
} }
} } Optical Emission Spectroscopy - I'm not too familiar with this
technique,
} } but I seem to recall they will often use a spark source in the
process.
} } When they do that, I think this is then called Spark Emission
} } Spectroscopy. You might want to check out Bruker AXS Microanalysis, I
} } believe they make some of these instruments.
} }
} } Perhaps if you post what they are testing and what they want from the
} } analysis, that may help. I hope I've given you at least some food for
} } thought.
} }
} } Good luck,
} }
} } dj
} }
} }
} }
} } On Thu, 18 Jan 2007, TindallR-at-missouri.edu wrote:
} }
} } }
} } }
} } }
} } }
} }
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} } } To Subscribe/Unsubscribe --
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} } }
} }
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} } }
} } } And along a completely different line, we've been asked if we can do
a
} } } "spark emission test". Since I don't know what that is, and Google
} } } hasn't helped, I don't know if we can or not.
} } }
} } } Has anybody heard of this test? Seems to be a way of checking
sample
} } } composition at concentrations below those attainable by EDS, but
that's
} } } about all I can tell.
} } }
} } } Sorry if this isn't microscopy-related, but I don't even know that,
at
} } } this point. We'll see what the collective comes up with on this
one!
} } }
} } } Thanks to all.
} } }
} } } Randy
} } }
} } } Randy Tindall
} } } Senior EM Specialist
} } } Electron Microscopy Core Facility---We Do Small Well!
} } } W125 Veterinary Medicine
} } } University of Missouri
} } } Columbia, MO 65211
} } } Tel: (573) 882-8304
} } } Fax: (573) 884-2227
} } } Email: tindallr-at-missouri.edu
} } } Web: http://www.emc.missouri.edu
} } } On-line calendar:
} } }
http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=
} } } Week&NavType=Both&Type=TimePlan
} } }
} } }
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From: r-holdford-at-ti.com
Date: Fri, 19 Jan 2007 17:40:59 -0600
Subject: [Microscopy] Re: viaWWW: EBL/ PMMA Mask

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Kurt: Since my company banned cyanide-based etches some years back. one
of my favorite gold etches is as follows:

4.6 grams potassium iodide
1.3 grams Iodine
100 milliliters of DI water
(ratios can be increased/decreased as needed)

Use at room temp. This removes 2-4 microns of Au in around 5 minutes,
according to my notes. You might want to test it on something
expendable before you use it on the real deal. It will store a long time.

klangwor-at-uoregon.edu wrote:
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} Email: klangwor-at-uoregon.edu
} Name: Kurt Langworthy
}
} Organization: University of Oregon
}
} Title-Subject: [Filtered] EBL/ PMMA Mask
}
} Question: All,
} I'm looking for a gold etching recipt that will allow me to use PMMA as a mask. KCN looks promising, but is highly toxic. Does anyone have any better ideas?
} Thanks,
} Kurt
}
} ---------------------------------------------------------------------------
}
} ==============================Original Headers==============================
} 6, 12 -- From zaluzec-at-microscopy.com Wed Jan 17 18:34:22 2007
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--
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Becky Holdford (r-holdford-at-ti.com)
972-995-2360
972-648-8743 (pager)
SC Packaging FA Development
Texas Instruments, Inc.
Dallas, TX
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~


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From: jeremy.wilburn-at-gmail.com
Date: Sat, 20 Jan 2007 09:27:38 -0600
Subject: [Microscopy] viaWWW: biological microscopy question

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Email: jeremy.wilburn-at-gmail.com
Name: Jeremy Wilburn

Organization: Vanderbilt University

Title-Subject: [Filtered] biological microscopy question

Question: I am a chemistry grad student working with both live cells and cultured pancreatic islets. More specifically I do electrochemical (electrophysiology) measurements with ultramicroelectrodes, and am needing to put together an instrument similar to a patch-clamp on an inverted microscope. I am in the market for an inverted microscope now, but am limited in funding (~$8-10k).

My problem is the following. Since I use a capillary electrode body (which must remain completely vertical, unlike a patch pipette), I am unable to use the top condenser for imaging (due to both space restraints and shadowing from the electrode body). I was thinking to get a fluorescence-type inverted microscope and remove the filter, making a bottom-illumination similar to a metallurgical microscope, but would still let me do fluorescence at some point...would this work?

My second option would be a more expensive scope (like Olympus IX71) that has multiple light ports...

Any other suggestions (including a good scope to look for) would be greatly appreciated.

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From: Remi.Delville-at-ua.ac.be
Date: Sat, 20 Jan 2007 09:28:00 -0600
Subject: [Microscopy] viaWWW: Electropolishing TiNiAu

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Email: Remi.Delville-at-ua.ac.be
Name: RÈmi DELVILLE

Organization: EMAT, University of Antwerp, Belgium

Title-Subject: [Filtered] Electropolishing TiNiAu

Question: Dear microscopists,

I am currently looking for a suitable electropolishing solution for the ternary shape-memory alloys TiNiAu in order to have a thinned sample for electron microscopy.

I already tried several solutions that are known to work well with TiNi. Unfortunately, the added gold at a concentration around 15 at% seems to severely hamper the electro-polishing: the same conditions as for Ni-Ti give etched specimens with bad edges of the hole.
We've played with tension, temperature, fluid speed,etc.. but without success.


Solutions tried that work with NiTi but not with my sample:

80% methanol + 20% sulphuric acid (-17ƒC)
93% acetic acid + 7% perchloric acid (5ƒC)
93% methanol + 7% perchloric acid (-10ƒC)
75% methanol + 25% nitric acid (-22ƒC)

I already looked up in the literature and couldn't find anything close to my alloy. So I was wondering if you might have a suggestion of an alternative, eg, adding an extra acid to one of the above baths or maybe something completely different.

Many thanks in advance,


RÈmi Delville

EMAT - Electron Microscopy for Material Science - University of Antwerp, Belgium



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From: peter.tomic-at-renwireless.com
Date: Sat, 20 Jan 2007 09:28:30 -0600
Subject: [Microscopy] viaWWW: FEI XL-50 Experience

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Email: peter.tomic-at-renwireless.com
Name: Peter Tomic

Organization: Renaissance Wireless

Title-Subject: [Filtered] FEI XL-50 Experience

Question: Listers,

I'd like to hear from anyone that has owned/operated an FEI XL50 SEM. I have one we are bringing up in our MEMS lab. There are some unusual aspects to this instrument such as the exchange port.

My understanding is that there were not very many XL-50's made. Does anyone currently own one?

You may respond on-list or off-list if you care to.

Peter Tomic
Renaissance Wireless Corp.
Somerset, New Jersey, USA


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From: liang-at-saturn.med.nyu.edu
Date: Sat, 20 Jan 2007 09:29:10 -0600
Subject: [Microscopy] viaWWW: Job Opening Research Technician in EM core facility

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Email: liang-at-saturn.med.nyu.edu
Name: Alice Liang

Organization: NYU school of Medicine

Title-Subject: [Filtered] Research Technician in EM core facility

Question: Research Technician ñ Electron Microscopy
The Image Core Facility of Skirball Institute, NYU School of Medicine

A full - time research technician position is currently available in the
Image Core Facility at Skirball Institute of NYU School of Medicine. The
facility provides essential electron microscopic and structural analysis
support for school of medicine and local community. The successful candidate
must have at least a B.S. degree, appropriate training and significant
biomedical research experience in histology, electron microscopy (TEM) and
tissue sample preparation. Candidates with an excellent communication skill
and experience in instrument maintenance are encouraged to apply. Skirball
is an academic organization and offers competitive salaries and excellent
fringe benefits. Interested individuals should send resume and three
references to:

Alice Liang, Ph.D.
Director of Image Core Facility
Skirball Institute of Biomolecular Medicine
Skirball 2nd floor, EM Suite
NYU School of Medicine
New York, NY 10016
Tel: 212-263-7644 (o); 212-263-7099 (Lab)
Fax: 212-263-7643
E-mail: liang-at-saturn.med.nyu.edu
http://saturn.med.nyu.edu/facilities/imagecore/

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From: rafaldb-at-gmail.com
Date: Sat, 20 Jan 2007 09:30:34 -0600
Subject: [Microscopy] viaWWW: Positions available: Technical University of Denmark

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Email: rafaldb-at-gmail.com
Name: Rafal Dunin-Borkowski

Organization: Technical University of Denmark

Title-Subject: [Filtered] Positions available: Technical University of Denmark

Question: Immediate opening:

-----------------------------------------------------------------
Senior scientists in Electron Microscopy at the Center for Electron Nanoscopy, Technical University of Denmark

Apply before: 12.02.2007 at 12:00 (noon)

The Center for Electron Nanoscopy (CEN) at the Technical University of Denmark (DTU), which has been made possible by a generous donation from the A.P. Moller Foundation, seeks to appoint one or more senior scientists to permanent positions in advanced electron microscopy for materials science and nanotechnology. The number of appointments will depend on the quality and experience of the applicants.

CEN-DTU will be fully operational in 2007 and will contain seven new electron microscopes. There will be two aberration-corrected monochromated TEMs, one of which will be equipped with a gas reaction capability.

Applicants must have outstanding international research reputations and hands-on expertise in a wide range of advanced electron microscopy techniques. They will be expected to perform experimental work with users of the facility, and to assist CEN's director in raising research funding and in developing an internationally leading program of advanced research.

Expertise in one or more of the following areas is essential: gas reaction TEM; aberration-correction; monochromated EELS; electron tomography; electron holography; direct methods; advanced image analysis and simulation for quantitative electron microscopy.

Positions are to be filled in 2007, on dates to be agreed with the successful applicants.

The salary and appointment terms will be based on the current collective agreement for Danish University faculty members.

All interested candidates, irrespective of age, gender, race, religion and ethnic background, are invited to apply.

Further information can be obtained from CEN's director Rafal Dunin-Borkowski (rafaldb-at-gmail.com).

Applications, which should include a resumÈ, list of publications and statement of research interests, should be sent to

The Rector
Technical University of Denmark
Building 101 A
DK-2800 Lyngby, Denmark

and must be received before 12:00 on 12 February 2007.

-----------------------------------------------------------------


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From: dac-at-research.umass.edu
Date: Sat, 20 Jan 2007 09:32:13 -0600
Subject: [Microscopy] viaWWW: Sputter coating details

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Email: dac-at-research.umass.edu
Name: Dale Callaham

Organization: University of Massachusetts

Title-Subject: [Filtered] Sputter coating details

Question: Hi listees,

This message is in response to the thread about Polaron E5100 sputter coaters and operating conditions: HV knob setting and voltages to be used. I saw mention that finer grain size of the sputtered film is achieved when a lower voltage (I think 700V was mentioned) is used, and I have seen this elsewhere. I'm trying to come to terms with this and sometimes it is worth going back to basics.

Several sources show graphs of the Voltage and Current relationships in a DC plasma:

http://science-education.pppl.gov/SummerInst/SGershman/Structure_of_Glow_Discharge.pdf
(Sophia Gershman must be one heck of a High School teacher!)

http://www.emitech.co.uk/sputter-coating-brief2.htm

These data show that for the range of conditions typically used in a sputter coater the actual voltage that exists in the plasma is clamped by the physics of the plasma and while the knob may set 700V or 2.2kV, the difference between the set voltage and the voltage across the plasma must be accounted for by IR drop in the winding resistance and any ballasting/limiting resistors in the circuit (the E5100 has resistance in the primary leads, 5k ohm in the secondary lead, plus the considerable winding resistance of the secondary HV winding). The description of the behaviour of the plasma is that the current increases by increasing the volume of the plasma - any you can see this for yourself - but as best I can judge by the graphs in the referenced papers, the voltage may increase only very slightly, and it is difficult for me to see that it would change the energy of electrons bombarding the target significantly.

Is the "finer grain with lower voltage" real? If it is real, what is the basis for it? Have I completely misinterpreted the data?

Sincerely,

Dale Callaham
Umass, Amherst
dac-at-research.umass.edu



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From: edelmare-at-muohio.edu
Date: Sat, 20 Jan 2007 14:54:54 -0600
Subject: [Microscopy] Re: viaWWW: biological microscopy question

Contents Retrieved from Microscopy Listserver Archives
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Jeremy:

The reflected light imaging will work, but your biggest issue will
be contrast in the biological samples. And for reflected light
imaging you do NOT need or want a high brightness light source like
those used for florescence work. Cost savings ($100 vs $5000).

As for contrast reflected light DIC might be ideal however since it
has depth of focus issues seeing the electrode as it approaches the
cell would be problematic. And it is costly. I suggest you look at
reflected light dark-field. A rare beast but might be what you need.

Another approach would be oblique illumination. I seem to remember
that some inverted scopes had/have tilting lamp/condensors so you can
tilt them off axis 35? 45? 60-degrees or so. . . . in fact I just
went and checked my IX-81 illumination column tilts backwards, and
the manual states "Even with the illumination column tilted the
specimen surface will be illuminated, which is convient for rough
confirmation of the specimen location on initial positioning of the
specimen." My guess would be that setting Köhler illumination for
highest resolution would be an issue but would still allow very
reasonable bright-field imaging.

good luck.




On 20 Jan 2007 at 9:29, jeremy.wilburn-at-gmail.com wrote:

}
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} Name: Jeremy Wilburn
}
} Organization: Vanderbilt University
}
} Title-Subject: [Filtered] biological microscopy question
}
} Question: I am a chemistry grad student working with both live cells and cultured pancreatic islets. More specifically I do electrochemical (electrophysiology) measurements with ultramicroelectrodes, and am needing to put together an instrument similar to a patch-clamp on an inverted microscope. I am in the market for an inverted microscope now, but am limited in funding (~$8-10k).
}
} My problem is the following. Since I use a capillary electrode body (which must remain completely vertical, unlike a patch pipette), I am unable to use the top condenser for imaging (due to both space restraints and shadowing from the electrode body). I was thinking to get a fluorescence-type inverted microscope and remove the filter, making a bottom-illumination similar to a metallurgical microscope, but would still let me do fluorescence at some point...would this work?
}
} My second option would be a more expensive scope (like Olympus IX71) that has multiple light ports...
}
} Any other suggestions (including a good scope to look for) would be greatly appreciated.
}
} ---------------------------------------------------------------------------
}
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Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Director
364 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu
http://www.emf.muohio.edu

"A main-frame: The biggest PC peripheral available."



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From: mgb-at-ansto.gov.au
Date: Mon, 22 Jan 2007 08:03:07 -0600
Subject: [Microscopy] viaWWW: Wanted: anode chamber for JEOL 2000fxII TEM

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Email: mgb-at-ansto.gov.au
Name: Mark Blackford

Organization: Australian Nuclear Science and Technology Organisation

Title-Subject: [Filtered] Wanted: anode chamber for JEOL 2000fxII TEM

Question: Hi All,

I'm hoping someone may be able to help locate a working anode chamber (electron gun) for a JEOL 2000fxII TEM. Our microscope can only be operated at 100kV due to electrical discharging in the anode chamber at higher accelerating voltages. Several attempts at cleaning the internal components have not been entirely successful.

A fully refurbished unit can be supplied by JEOL, but not cheaply.

I would like to contact anyone who has a suitable fully functional anode chamber, capable of working at 200kV, that they don't happen to need anymore. Please contact me off-line. Cheers,

Mark Blackford

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From: dmcdaniel-at-usuhs.mil
Date: Mon, 22 Jan 2007 08:03:29 -0600
Subject: [Microscopy] viaWWW: LR White polymerization problem

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Email: dmcdaniel-at-usuhs.mil
Name: Dennis McDaniel

Title-Subject: [Filtered] LR White polymerization problem

Question: I am having difficulty polymerizing a sample in LR White. The cells (macropahges and dendritic cells) were grown on a piece of aclar, fixed with formaldehyde/glutaraldehyde, post-fixed with uranyl acetate, dehydrated in a graduated series of EtOH, and infiltrated with LR White. I then sandwiched the aclar film containing the cells between two larger pieces of aclar (since the edges don't polymerize due to air exposure) and put the sample in the oven at 55C. After 48h, the resin immediately around the piece of aclar containing the cells appears very fragmented and cracked and is obviously unsuitable for sectioning, while the resin further from the cells appears to have polymerized properly. At first I thought it was an infiltration problem, but I did several changes of 100% resin and even left the samples overnight on a rotator at room temperature in 100% resin to ensure complete infiltration. Does anyone have any idea what they problem might be? Thanks.

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From: krensing-at-ucalgary.ca
Date: Mon, 22 Jan 2007 09:55:24 -0600
Subject: [Microscopy] Re: viaWWW: LR White polymerization problem

Contents Retrieved from Microscopy Listserver Archives
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Dennis,
I think that your problem is a result of incomplete dehydration. LR
White is supposed to handle some water without problem, but when you
sandwich the pieces, you create a limited volume for diffusion and the
water becomes too great locally. Make sure your ethanol is dry (on
molecular sieve or freshly opened).
That's my theory.
Kim

dmcdaniel-at-usuhs.mil wrote:

} Email: dmcdaniel-at-usuhs.mil
} Name: Dennis McDaniel
}
} Title-Subject: [Filtered] LR White polymerization problem
}
} Question: I am having difficulty polymerizing a sample in LR White. The cells (macropahges and dendritic cells) were grown on a piece of aclar, fixed with formaldehyde/glutaraldehyde, post-fixed with uranyl acetate, dehydrated in a graduated series of EtOH, and infiltrated with LR White. I then sandwiched the aclar film containing the cells between two larger pieces of aclar (since the edges don't polymerize due to air exposure) and put the sample in the oven at 55C. After 48h, the resin immediately around the piece of aclar containing the cells appears very fragmented and cracked and is obviously unsuitable for sectioning, while the resin further from the cells appears to have polymerized properly. At first I thought it was an infiltration problem, but I did several changes of 100% resin and even left the samples overnight on a rotator at room temperature in 100% resin to ensure complete infiltration. Does anyone have any idea what they problem might be? Thanks.
}
} ---------------------------------------------------------------------------


--
Kim Rensing Ph.D.
Manager,
Microscopy and Imaging Facility

University of Calgary,
Health Sciences Centre
B129 - 3330 Hospital Drive NW
Calgary, AB, Canada T2N 4N1
403-220-3488
krensing-at-ucalgary.ca

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From: gary.m.brown-at-exxonmobil.com
Date: Mon, 22 Jan 2007 11:22:02 -0600
Subject: [Microscopy] Re: viaWWW: LR White polymerization problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dennis,

I suspect that your polymerization problems arise from entrapped air. I
eliminate that problem by purging my oven with nitrogen, thus alleviating
other more tedious approaches. Works every time.

Regards,

Gary M. Brown
Microscopy Specialist
ExxonMobil Chemical Company
Baytown Technology & Engineering - West
5200 Bayway Drive
Baytown, Texas 77520-2101
phone: 281 834 2387
fax: 281 834 2395
e-mail: Gary.M.Brown-at-ExxonMobil.com



dmcdaniel-at-usuh
s.mil
To
gary.m.brown-at-exxonmobil.com
01/22/07 08:08 cc
AM
Subject
[Microscopy] viaWWW: LR White
Please respond polymerization problem
to
dmcdaniel-at-usuh
s.mil










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Email: dmcdaniel-at-usuhs.mil
Name: Dennis McDaniel

Title-Subject: [Filtered] LR White polymerization problem

Question: I am having difficulty polymerizing a sample in LR White. The
cells (macropahges and dendritic cells) were grown on a piece of aclar,
fixed with formaldehyde/glutaraldehyde, post-fixed with uranyl acetate,
dehydrated in a graduated series of EtOH, and infiltrated with LR White. I
then sandwiched the aclar film containing the cells between two larger
pieces of aclar (since the edges don't polymerize due to air exposure) and
put the sample in the oven at 55C. After 48h, the resin immediately around
the piece of aclar containing the cells appears very fragmented and cracked
and is obviously unsuitable for sectioning, while the resin further from
the cells appears to have polymerized properly. At first I thought it was
an infiltration problem, but I did several changes of 100% resin and even
left the samples overnight on a rotator at room temperature in 100% resin
to ensure complete infiltration. Does anyone have any idea what they
problem might be? Thanks.

---------------------------------------------------------------------------

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Headers==============================
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From: zerfasp-at-ors.od.nih.gov
Date: Mon, 22 Jan 2007 19:24:30 -0600
Subject: [Microscopy] viaWWW: Part for print processor

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Email: zerfasp-at-ors.od.nih.gov
Name: Patricia Zerfas

Organization: NIH

Title-Subject: [Filtered] Part for print processor

Question: Does anyone have a MohrPro8, model ME-42 print processor they are no longer using? I need to obtain the roller assembly that processes the print through the water and drying. My roller assembly is broken beyond repair and this model is no longer made. I am willing to pay for shipment.

Thanks,
Patricia Zerfas
National Institute of Health
Building 28A, Room 112
28 Library Drive
Bethesda, MD 20892 USA
ph # (301) 496-4464

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From: ptarq-at-evex.com
Date: Mon, 22 Jan 2007 19:35:08 -0600
Subject: [Microscopy] viaWWW: In memory of James Hillier

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Dear Colleagues:


I regret to inform you of the passing of fellow electron microscopist, friend, neighbor and mentor, James Hillier. James Hillier of Princeton, NJ developed the first operational electron microscope in 1938, died Monday January 15, 2007 at University Medical Center at Princeton. He was 91. He was a senior scientist and vice president at the Radio Corporation of America and a director of RCA's David Sarnoff Research Center.



As a graduate student at the University of Toronto in 1938, under Professor Eli Franklin Burton, Mr. Hillier and fellow student Albert Prebus designed and built the first north American commercial electron microscope prototype.



After obtaining a master's degree and a doctorate in 1941 from the University of Toronto, he was immediately hired by The Radio Corporation of America in Camden, where he developed the first commercially available electron microscope, later developing RCA's videodisc, a precursor to the DVD. He rose through the corporate ranks to become executive vice president for research and engineering and senior scientist at RCA in 1969. He also served as the director of the David Sarnoff Research Center in West Windsor.



Mr. Hillier held 41 patents for devices and processes, the most significant for the electron microscope. After receiving a joint award from the American Public Health Association and the Albert and Mary Lasker Foundation for medical research in 1960, Mr. Hillier said to a reporter from Time magazine, "The electron microscope is like the monkey wrench on the garage wall; what you do with it is the important thing."



After retiring from RCA in 1977, he focused his attention on the National Inventors Hall of Fame in Akron, Ohio, and later The James Hillier Foundation for Science Education, dedicated to funding the college education of bright scientifically oriented students. In 1993, he established the James Hillier Foundation, which awards scholarships each year to science students from Brant County, Ontario.



Dr. Hillier received honorary degrees from New Jersey Institute of Technology and the University of Toronto. A public school in Brantford, Ontario, his birthplace, has been named in his honor. He was one of the first scientists elected to the National Inventors Hall of Fame in 1980. In 1997, he was made officer of the Order of Canada, the country's highest civilian honor.



His late wife owned and managed the Flower Basket in Princeton and later owned two additional Princeton flower shops. Both artists, Mr. Hillier made photo-realistic pastels and drawings while his wife was an abstract artist and an award-winning flower arranger. Son of the late James and Ethel Cooke Hillier, husband of the late Florence M. Bell Hillier, who died in 1992 after 55 years of marriage, father of the late William W. Hillier, who died in 2002, he is survived by his son, architect James Robert Hillier of New Hope, Pa.; sisters May Hillier of Brantford, Ontario, and Thelma Henshaw of Naples, Fla.; three grandsons; a granddaughter; and three great-grandchildren.



In lieu of flowers, memorial contributions may be made to the James Hillier Foundation, 34 Hill Ave., Brantford, Ontario, Canada, N3R 4HI. {http://comdir.bfree.on.ca/hillier/index.html} http://comdir.bfree.on.ca/hillier/index.html





Sincerely,

Peter P. Tarquinio











Evex Inc.,
Peter Tarquinio
857 State Road
Princeton, NJ 08540

Telephone - 609-252-9192
Fax - 609-252-9091
E-mail - {mailto:ptarq-at-evex.com} ptarq-at-evex.com
{http://www.evex.com} http://www.evex.com









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From: LarryZ-at-fai.us
Date: Tue, 23 Jan 2007 13:04:08 -0600
Subject: [Microscopy] Rebuilding Amray 1850 FE Gun

Contents Retrieved from Microscopy Listserver Archives
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We are re-building and replacing the gun FE tip on an Amray 1850 FE.
Question: What is the tolerance, i.e., the distance from the suppressor
to the extractor? We have a sketch showing 0.765mm, but no plus or minus
tolerance. Does anyone know what the tolerance should be?

Thank you for your help.

Larry Zagorski
Sr. Metallurgist
FAI Materials Testing Laboratory
825 Chance Road
Marietta, GA 30066
770-928-1930
larryz-at-fai.us



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From: jehrman-at-mta.ca
Date: Wed, 24 Jan 2007 12:26:27 -0600
Subject: [Microscopy] instructions: Vigor TW-1000 tweezer sharpener

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Greetings listers,

I was going through some cabinets and came across a Vigor TW-1000
tweezer sharpener (sold by Pella, apparently, a long time ago, judging
by the yellowing of the box) sans instructions. I think I've figured out
how the thing works, but would like a copy of the instructions to see if
I have all the parts and all those parts in the correct location. Is
there anyone out there in the ether who has a copy they could scan,
xerox, or otherwise send to me? The beer is on me and I'll show you the
fantastically high tides if you ever find yourself in Sackville, New
Brunswick (doesn't everybody wish they were here?).

Thank in advance,

Jim

--

James M. Ehrman
Digital Microscopy Facility
Mount Allison University
Sackville, NB E4L 1G7
CANADA

phone: 506-364-2519
fax: 506-364-2505
email: jehrman-at-mta.ca
www: http://www.mta.ca/dmf


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From: msteglic-at-mdanderson.org
Date: Wed, 24 Jan 2007 13:14:16 -0600
Subject:

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Did you try contacting Ted Pella? They may be able to help.

Mannie Steglich





jehrman-at-mta.ca

01/24/2007 12:30 PM
Please respond to jehrman




To:
msteglic-at-mdanderson.org
cc:






Greetings listers,

I was going through some cabinets and came across a Vigor TW-1000
tweezer sharpener (sold by Pella, apparently, a long time ago, judging
by the yellowing of the box) sans instructions. I think I've figured out
how the thing works, but would like a copy of the instructions to see if
I have all the parts and all those parts in the correct location. Is
there anyone out there in the ether who has a copy they could scan,
xerox, or otherwise send to me? The beer is on me and I'll show you the
fantastically high tides if you ever find yourself in Sackville, New
Brunswick (doesn't everybody wish they were here?).

Thank in advance,

Jim

--

James M. Ehrman
Digital Microscopy Facility
Mount Allison University
Sackville, NB E4L 1G7
CANADA

phone: 506-364-2519
fax: 506-364-2505
email: jehrman-at-mta.ca
www: http://www.mta.ca/dmf


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From: jehrman-at-mta.ca
Date: Wed, 24 Jan 2007 13:14:33 -0600
Subject: [Microscopy] Thanks: instructions: Vigor TW-1000 tweezer sharpener

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Listers,

After the usual glut of "Out of Office" replies (gotta love those
people -- can't shoot 'em) I have a
contact who'll send me a copy of the instructions. Thanks for the other
offers.

Cheers,

Jim

--

James M. Ehrman
Digital Microscopy Facility
Mount Allison University
Sackville, NB E4L 1G7
CANADA

phone: 506-364-2519
fax: 506-364-2505
email: jehrman-at-mta.ca
www: http://www.mta.ca/dmf


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From: TindallR-at-missouri.edu
Date: Wed, 24 Jan 2007 13:44:05 -0600
Subject: [Microscopy] TEM: Fixation of mouse brain tissue

Contents Retrieved from Microscopy Listserver Archives
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Dear Listers,

Does anyone have any pet techniques/fixatives for TEM processing of
brain tissue? Specifically mouse brain, if it matters. We just did a
run with less than stellar results----poor membranes, etc. We are now
trying a couple different things, but if anyone has some tried-and-true
tips, it might save us a ton of time. No immunolabeling, just
ultrastructure.

Some recipes call for picric acid. Can anyone tell me what purpose this
serves?

Thanks a heap, as usual!

Cheers,
Randy

Randy Tindall
Senior EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W125 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu
On-line calendar:
http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=
Week&NavType=Both&Type=TimePlan


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From: bozzola-at-siu.edu
Date: Wed, 24 Jan 2007 14:09:41 -0600
Subject: [Microscopy] Re: TEM: Fixation of mouse brain tissue

Contents Retrieved from Microscopy Listserver Archives
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CNS tissue is best fixed by perfusion, without a doubt. Conventional
procedures (excision) hardly ever give very good results with CNS.

So, my first question is: did you use perfusion?

JB


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From: mcauliff-at-umdnj.edu
Date: Wed, 24 Jan 2007 14:30:54 -0600
Subject: [Microscopy] Re: TEM: Fixation of mouse brain tissue

Contents Retrieved from Microscopy Listserver Archives
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Hi Randy:

I use 2-3% glutaraldehyde in 0.1M phosphate buffer at room temp., pH
7.3-7.4, perfused via the left ventricle. While I have a small
peristaltic pump I have used gravity with excellent results. While some
will argue otherwise, one does need a fancy apparatus or high pressure,
I don't even measure the pressure. I use just a few ml of the same
buffer (without fancy additives) as a washout and at least 50-75 ml of
fix perfused in 5 minutes. In my experience most labs with less than
optimal results use 1. a tiny little needle in the ventricle 2. an
inadquate rate of flow and 3. way too much washout. Use an 18-20 g
needle (same inside diameter as the aorta!) inserted into the vent. then
have the assistant just nick the r. atrium, being careful not to go too
deep and cut the aorta .......-at-#$%^!! Going too far into the vent. can
be a problem so put a piece of cork or tygon tubing aroung the shaft of
the needle so you don't go too deep. Make everything fresh. Fix for a
few hours at room temp., multiple rinses in buffer, osmicate (reasonably
fresh osmium) thin slices/small pieces in the same buffer for several
hours, multiple buffer rinses, store in buffer if needed. If you must
keep orientation cut 200 micron Vibratome sections (before osmication)
so you can see the antomy after osmium turns everything black.
Dehydrate rapidly in graded ethanols (too much time in ethanols will
remove cytoplasm, Hyatt's book has the illustrations and refs. to prove
this), clear, infiltrate and embed. Uranyl acetate (after multiple
washes in sodium acetate to remove phosphates) will improve contrast. I
have not used K-ferricyanide to improve osmium staining.
Some labs use a little bit of picric acid as a protein coagulator I
suppose. Plenty of people get good results without it.

Geoff


TindallR-at-missouri.edu wrote:

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From: smythen-at-musc.edu
Date: Wed, 24 Jan 2007 18:40:45 -0600
Subject: [Microscopy] viaWWW: vigor tweezer sharpener

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Email: smythen-at-musc.edu
Name: Nancy Smythe

Organization: Medical University of South Carolina

Title-Subject: [Filtered] vigor tweezer sharpener

Question: Good luck. I bought one about 6 years ago without instructions. But I do get results, so if you don't get the instructions let me know and I will tell you my techniques and you can share what you know and between the two of us we can maybe get it straight!

---------------------------------------------------------------------------

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From: tivol-at-caltech.edu
Date: Wed, 24 Jan 2007 19:02:21 -0600
Subject: [Microscopy] Re: viaWWW: vigor tweezer sharpener

Contents Retrieved from Microscopy Listserver Archives
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On Jan 24, 2007, at 4:40 PM, smythen-at-musc.edu wrote:

} Good luck. I bought one about 6 years ago without instructions. But
} I do get results, so if you don't get the instructions let me know and
} I will tell you my techniques and you can share what you know and
} between the two of us we can maybe get it straight!
}
Dear Jim & Nancy,
Could you please post your techniques to the list? TIA.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu


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From: r.sims-at-auckland.ac.nz
Date: Wed, 24 Jan 2007 19:30:07 -0600
Subject: [Microscopy] 840 vacuum problem

Contents Retrieved from Microscopy Listserver Archives
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Here's one for all the 840 afficianados:

In the 'ready' state all is well, with all the valve lights as they should be, and a
pressure of around 10 - 5 in the chamber (there are 3 WDS spectros leaking away
into the chamber) and around 5 x 10 - 6 in the gun.

When I push the GUN button to vent the gun, the lights do what they should, including
the opening of LV1, but the gun pressure comes not up to atmospheric but to about
100 mbar, and the pressure in the vacuum ballast tank starts climbing, until the PiG3
light comes on and the system devotes itself, as it should, to re-evacuating the ballast
tank.

Am I correct in guessing that V1B, the gun pumpdown valve, is not closing properly
and is allowing the atmosphere to leak via LV1, the gun, and V1B, into the ballast
tank?

I would welcome support (or, I suppose, otherwise) in this guess before I start ripping
things apart.

There is a time constraint here, as we have the NZ EM conference in 10 days time
and there's to be a teaching workshop involving the 840.

Help!

cheers

rtch

--
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Microanalyst Fax : 64 9 3737435
Department of Geology email : r.sims-at-auckland.ac.nz
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Auckland
New Zealand


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From: jzheng-at-uci.edu
Date: Wed, 24 Jan 2007 20:38:58 -0600
Subject: [Microscopy] Gatan ion mill

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi,
I need an easy and quick method to clean the anode in Gatan dual mill.
Could you pleasse help? Thanks, Jeffrey



==============================Original Headers==============================
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From: peter.heimann-at-uni-bielefeld.de
Date: Thu, 25 Jan 2007 02:46:56 -0600
Subject: [Microscopy] Re: TEM: Fixation of mouse brain tissue

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Randy,
Geoff McCauliff's tips are my experiences too.
a minute comment: use gravity perfusion; a wonderful & detailed
instruction is given in:

Forssmann, W.G., Ito, S., Weihe, E., Aoki, A., Dym, M. and Fawcett,
D.W. (1977) Anat Rec 188, 307-14.

a small but important tip: Filtrate ALL solutions used for perfusion
through a 1 (or 2) mikrometer filter before use!

If you ever have seen a capillary in cross section and measured its
diameter, you know why.

Use the two resp. tree-step technique described in the paper, in my
experience perfusion with the "rinse" solution for 20-30 seconds
followed by a single or two-step perfusion (3-5 minutes) with a mixture
of freshly (!) prepared form- and glutaraldehyde (the latter prepared
by the manufacturer according to the Anderson-technique) is sufficient
for an adult wildtype mouse.

good luck,
peter heimann


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From: cammer-at-aecom.yu.edu
Date: Thu, 25 Jan 2007 10:17:50 -0600
Subject: [Microscopy] color CCD Zeiss AxioCam MRc problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi

Vacuum systems have high and low speed pumping areas their problems show up
as set out below, high (1) and low (2).

1. If a reasonable vacuum is held in the high vacuum area but the rotary
pump or the backing tank show a considerable decrease in their vacuum level,
the problem is in an area with close proximity to a main pumping line

2. If a poor vacuum is held in the high vacuum area and the rotary pump or
the backing tank also show a considerable decrease in its vacuum level, the
problem is in an area with some distance (vacuum wise) to a main pumping
line.

Always ask what did I do last and it seems that here lies the problem, first
reverse the action does the system recover? It would suggest a leak
between the gun, trying to reach atmospheric, and the column trying to
retain its high vacuum.

Good luck

Steve Chapman
Senior Consultant Protrain
For electron microscopy consultancy and training world wide
Tel +44 1280 816512 Fax +44 1280 814007
Mobile +44 7711 606967 Web www.emcourses.com


----- Original Message -----
X-from: {r.sims-at-auckland.ac.nz}
To: {protrain-at-emcourses.com}
Sent: Thursday, January 25, 2007 1:30 AM

We found that the red (typically rhodamine) images taken with our
Zeiss Axiocam MRc are fuzzy. We looked at the blue and found this a
problem too, but since in fluorescence people typically use the blue
just as a marker for nucleii rather than as stain for fine structure,
they didn't notice this problem. (Illustrations at
http://www.aecom.yu.edu/aif/temp/axiocamproblem/ ).

This made us worry about the sharpness of images of histological
stained samples, such as H&E.

First we checked that this isn't a problem due to the fluorescent
filter sets. To do this, we filtered the light to be red, green or
blue at the halogen lamp using RGB filters removed from an old 35mm
film recorder and imaged the sample with no filters in the light path
between the sample and the camera. The result was that the red and
blue images were very fuzzy.

Shooting in the b/w mode solves this problem, but then the images are
not color. Also, this may solve the problem for fluorescence, but
what about brightfield or other techniques that involve colors?

Can anyone explain why this happens and how to fix it? Is this a
problem with our sytem uniquely or is this a problem with the Axiocam
in general?

Thanks!

-Michael
____________________________________________________________________________
Michael Cammer Analytical Imaging Facility Albert Einstein Coll. of Med.
URL: http://www.aecom.yu.edu/aif/


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From: wim5-at-lehigh.edu
Date: Thu, 25 Jan 2007 11:05:58 -0600
Subject: [Microscopy] Re: 840 vacuum problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

If you use the "VENT" button rather than the "GUN VENT" to vent the
whole column and the ballast pressure holds up, your problem is most
likely that the V7 valve O-ring is leaking between the gun and
specimen chambers. This would also eliminate V1B as a possibility.

Unfortunately, you will have to tear down the column to get to the V7
O-ring. The good news is, this valve should only present a problem
when doing a gun vent. To avoid introducing more serious problems I
would just use the VENT button temporarily when needed and postpone
the repair until after your conference.

Regards,
Bill

On 1/24/07, r.sims-at-auckland.ac.nz {r.sims-at-auckland.ac.nz} wrote:
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
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} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
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}
}
} Here's one for all the 840 afficianados:
}
} In the 'ready' state all is well, with all the valve lights as they should be, and a
} pressure of around 10 - 5 in the chamber (there are 3 WDS spectros leaking away
} into the chamber) and around 5 x 10 - 6 in the gun.
}
} When I push the GUN button to vent the gun, the lights do what they should, including
} the opening of LV1, but the gun pressure comes not up to atmospheric but to about
} 100 mbar, and the pressure in the vacuum ballast tank starts climbing, until the PiG3
} light comes on and the system devotes itself, as it should, to re-evacuating the ballast
} tank.
}
} Am I correct in guessing that V1B, the gun pumpdown valve, is not closing properly
} and is allowing the atmosphere to leak via LV1, the gun, and V1B, into the ballast
} tank?
}
} I would welcome support (or, I suppose, otherwise) in this guess before I start ripping
} things apart.
}
} There is a time constraint here, as we have the NZ EM conference in 10 days time
} and there's to be a teaching workshop involving the 840.
}
} Help!
}
} cheers
}
} rtch
}
} --
} Ritchie Sims Ph D Phone : 64 9 3737599 ext 87713
} Microanalyst Fax : 64 9 3737435
} Department of Geology email : r.sims-at-auckland.ac.nz
} The University of Auckland
} Private Bag 92019
} Auckland
} New Zealand
}
}
} ==============================Original Headers==============================
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}


--
William J Mushock
Lehigh University
Whitaker Laboratory
5 East Packer Ave.
Bethlehem, PA 18015
(610)-758-4283

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From: ani.issaian-at-csun.edu
Date: Thu, 25 Jan 2007 11:14:16 -0600
Subject: [Microscopy]

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi everyone,

I am trying to order photographic paper racks which are used inside a dryer box after printing. If anyone knows were I can buy them, please let me know.
Thanks,


ANI M ISSAIAN
Electron Microscopy Facility Manager
California State University, Northridge
18111 Nordhoff street, Northridge, CA 91330-8303
Biology dept., MC 8303, Room CS2205
Phone: (818) 677-3383
Fax (818) 677-2034

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From: donovan-at-uoregon.edu
Date: Thu, 25 Jan 2007 11:24:37 -0600
Subject: [Microscopy] Nitrogen free epoxy?

Contents Retrieved from Microscopy Listserver Archives
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All,
Can anyone tell me of a source for a nitrogen free epoxy? I realize
that may be a contradiction in terms, but I need to find a mounting
media that can hold a water soluble polymer coated wire for cross
sectioning for subsequent nitrogen analysis by EPMA.

Thanks!
john

John J. Donovan donovan-at-uoregon.edu
University of Oregon (541) 346-4632 (office)
1260 Franklin Blvd (541) 346-4655 (probe)
Eugene, OR (541) 346-4778 (SEM)
97403-1272 (541) 346-4692 (FAX)

Lab Web: http://epmalab.uoregon.edu/
EPMA (SX100)Schedule: http://sweetwater.uoregon.edu/sx100
EPMA (SX50) Schedule: http://sweetwater.uoregon.edu/epma
SEM (Ultra) Schedule: http://sweetwater.uoregon.edu/zeiss
SEM (Quanta) Schedule: http://sweetwater.uoregon.edu/sem
Remote Access: http://epmalab.uoregon.edu/howto.htm
Personal: http://www.uoregon.edu/~donovan/

"Aristotle presented with a moon rock would have no difficulty in
discerning it as an object not fundamentally different from
terrestrial materials. So much for Thomas Kuhn."

"I'd rather be uncertain and wrong, than be certain and wrong."




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From: pli-at-dal.ca
Date: Thu, 25 Jan 2007 12:48:53 -0600
Subject: [Microscopy] WTB: an used Acer Altos computer for TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Our current computer (Acer Altos 11000) that controls FEI's Tecnai 12 TEM is
having serious problems. I am looking for an used Acer Altos 11000, or
12000, or 21000 to replace our current one. If you have one for sale or if
you would like to upgrade yours, could you please let me know? I will pay
for the computer, handling and shipping cost.

Thank you,
Ping

--
Ping Li, Ph.D.
Director, Scientific Imaging Suite
Department of Biology
Dalhousie University
Halifax, NS B3H 4J1
Canada

Tel: 902-494-3309
Fax: 902-494-3736
E-mail: Ping.Li-at-Dal.Ca



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From: jehrman-at-mta.ca
Date: Thu, 25 Jan 2007 13:25:22 -0600
Subject: [Microscopy] JEOL 5xxx photo CRT

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi all,

With somewhat mixed emotions, we finally decommissioned our darkroom and
transferred
the (nearly new) 4x5 setup to the Fine Arts Department. This move was
prompted by
the upgrade of our JEOL 5600 computer system, which essentially disabled
the photo system
(not that we ever used it). On a recent service visit I had the engineer
remove the system entirely.
JEOL Canada doesn't have any interest in retrieving it, so on the off
chance that somebody desperately
needs a replacement, I'm offering it to anyone who will pay shipping for
the thing. Let me know
relatively quickly - it's probably destined for the dumpster if I can't
find someone to give it to.
There's a new Polaroid back on it, if that makes it any more attractive.

Cheers,

Jim

--

James M. Ehrman
Digital Microscopy Facility
Mount Allison University
Sackville, NB E4L 1G7
CANADA

phone: 506-364-2519
fax: 506-364-2505
email: jehrman-at-mta.ca
www: http://www.mta.ca/dmf


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From: lamoreaux-at-mail.csi.cuny.edu
Date: Thu, 25 Jan 2007 14:40:10 -0600
Subject: [Microscopy] research associate postion

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

The College of Staten Island of The City University of New York has an
opening for a Research Associate to serve as a Facilities Manager for the
Program in Neuroscience and Advanced Imaging Facility. A description of the
position can be found at:

http://www.csi.cuny.edu/administration/jobs/mp12932.pdf

Applications may sent to my attention:

William L'Amoreaux, Ph.D.
Associate Professor and Deputy Chair
Director, Advanced Imaging Facility
CUNY College of Staten Island
2800 Victory Blvd.
Staten Island, NY 10314
(718) 982-3864 - office
(718) 982-3852



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5, 20 -- To: {Microscopy-at-microscopy.com}
5, 20 -- Subject: research associate postion
5, 20 -- Date: Thu, 25 Jan 2007 15:40:05 -0500
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From: jbpawley-at-wisc.edu
Date: Thu, 25 Jan 2007 19:18:50 -0600
Subject: [Microscopy] Twelfth International UBC 3D LiveCell Course

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Twelfth Annual INTERNATIONAL 12-Day Short Course on 3D Microscopy of Living Cells, June 17 - 28, 2007 (Pre-course: June 16)

Eleventh, Post-course Workshop on 3D Image Processing, June 30 -July 2

Organized by Prof. James Pawley, (University of Wisconsin-Madison)

in association with the Departments of Pharmacology and Physiology and the Brain Research Centre,
University of British Columbia, Vancouver, BC, Canada

DATES

Applications must be received by Tuesday, March 15, 2007
Deposit due Friday, April 15, 2007
Registration 5:00 - 7:00 PM Saturday, June 16, 2007
First Lecture 7:30 PM Saturday, June 16, 2007
Live-cell Course ends, noon Thursday, June 28, 2007
3D Image Processing Course, Saturday, June 30 - Monday, July 2, 2007


APPLICATIONS DUE BY MARCH 15, 2007

APPLICATIONS
Applicants must complete a questionnaire on the web to assess knowledge level, field of interest and proposed personal, live-cell, project. But don't let this put you off: if you plan to use 3D microscopy on living cells, we can usually find a way to make it work.

Enrollment will be limited to about 32 participants (exact number depends on number of 3D Systems available). Selection will be made on the basis of background and perceived need. Those without previous LM experience will be provided with access to basic texts to read before the course begins. Application forms may be down-loaded from the WWW site at

http://www.3dcourse.ubc.ca/application.htm , or obtained from:

Prof. James B. Pawley, Ph. 608-263-3147
Room 223, Zoology Research Building, FAX 608-265-5315
250 N. Mills Street, Madison, WI, 53706 JBPAWLEY-at-WISC.EDU

Additional information is available from:
http://www.3dcourse.ubc.ca/brochure.htm and links.

We expect to have at least 13, 3D microscope workstations for student use and there will be an international faculty of 22.

Application deadlines:

Application forms should be received for screening by March 15, 2007. Successful applicants will be notified by April 1, and a deposit of 50% must be received by April 15, 2007. In general, refunds of the deposit will only be possible if someone on the waiting list can take your place but this has not been a problem in previous years. The remaining balance is due before Registration.


Pre-Course Tuition (1/2 day Basic Optical principles) $150 (US)
3D Live-cell Course Tuition (includes lunches, 2 big snacks, 3 dinners, incl. the
NEW Third Edition of the Handbook of Biological Confocal Microscopy): $2,850 (US)
Workshop Tuition (includes lunches, snacks, final dinner): $1,200 (US)

Room/board about $40/day (US) depending on room type.

I hope that this includes all of the information that you need, but if not, please get back to me.

Jim Pawley

--
****************************************
Prof. James B. Pawley, Ph. 608-263-3147
Room 223, Zoology Research Building, FAX 608-265-5315
1117 Johnson Ave., Madison, WI, 53706 JBPAWLEY-at-WISC.EDU
"A scientist is not one who can answer questions but one who can
question answers." Theodore Schick Jr., Skeptical Enquirer, 21-2:39

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From: kenconverse-at-qualityimages.biz
Date: Thu, 25 Jan 2007 19:43:36 -0600
Subject: [Microscopy] 840 vacuum problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi rtch,
The problem could well be V1B or V7 (column isolation). I haven't had much
opportunity to work on JEOL spectrometers, but the question arises: is
there a thin window detector and could its vacuum manifold be part of the
problem?

The part that bothers me the most is that the gun is being held to about
100mB (75 Torr) while being vented (this is a huge gas load to handle and
maintain that kind of pressure) and PiG 3 is ***not*** being dumped
instantaneously along with a big blast of DP oil being sent into the
specimen chamber.

I would be looking for a combination of a leak in V1B or V7 and either a
restriction in the nitrogen line to LV1 or perhaps its timer is shutting it
very quickly.

Btw what additional gauging have you got that would let you know that the
gun is at about 100mB? Do you have a convection gauge or Bourdon Tube gauge
attached to the gun?

I also strongly second Jim's comment about "what was the last thing you did
before you had the problem?", but I bet you already know that.

A very interesting problem. Keep us posted on what you find.

Ken Converse
owner

QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
474 So. Bridgton Rd.
Bridgton, ME 04009
207-647-4348
Fax 207-647-2688
kenconverse-at-qualityimages.biz
qualityimages.biz



-----Original Message-----
X-from: r.sims-at-auckland.ac.nz [mailto:r.sims-at-auckland.ac.nz]
Sent: Wednesday, January 24, 2007 8:33 PM
To: kenconverse-at-qualityimages.biz


Here's one for all the 840 afficianados:

In the 'ready' state all is well, with all the valve lights as they should
be, and a
pressure of around 10 - 5 in the chamber (there are 3 WDS spectros leaking
away
into the chamber) and around 5 x 10 - 6 in the gun.

When I push the GUN button to vent the gun, the lights do what they should,
including
the opening of LV1, but the gun pressure comes not up to atmospheric but to
about
100 mbar, and the pressure in the vacuum ballast tank starts climbing, until
the PiG3
light comes on and the system devotes itself, as it should, to re-evacuating
the ballast
tank.

Am I correct in guessing that V1B, the gun pumpdown valve, is not closing
properly
and is allowing the atmosphere to leak via LV1, the gun, and V1B, into the
ballast
tank?

I would welcome support (or, I suppose, otherwise) in this guess before I
start ripping
things apart.

There is a time constraint here, as we have the NZ EM conference in 10 days
time
and there's to be a teaching workshop involving the 840.

Help!

cheers

rtch

--
Ritchie Sims Ph D Phone : 64 9 3737599 ext
87713
Microanalyst Fax : 64 9 3737435
Department of Geology email :
r.sims-at-auckland.ac.nz
The University of Auckland
Private Bag 92019
Auckland
New Zealand


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_________________________________________________________________
Need personalized email and website? Look no further. It's easy
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From: nizets2-at-yahoo.com
Date: Fri, 26 Jan 2007 05:18:28 -0600
Subject: [Microscopy] Re: color CCD Zeiss AxioCam MRc problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi!

We have the same camera and I must say that I never
noticed that. We have the B/W axiocam too and since
this one is more sensitive than the color one, I
usually use it for fluorescence work. Of course each
wavelength having a specific convergence angle it is
necessary to refocus each time you change the filter,
however this does not explain why you get a sharp
image in BW mode (except if you refocused in this
mode).
The color camera is used for more "classical"
stainings like HE.
In general, I find the image pretty grainy. For
example the digital zoom is absolutely useless because
the image becomes too "pixely". Perhaps it is becaused
I am used to the high definition of TEM images,
perhaps it is because I do most of the LM work on
single cell imaging (reaching the physical limits of
LM). To answer this question rationally I should try a
higher resolution camera, however I am pretty sure I
won't have the credits just to answer this question
;-)
I questioned the Zeiss team about the quality of my
images and they say it is normal, so I have to accept
it somehow.

Regards,

Stephane

--- cammer-at-aecom.yu.edu wrote:

}
}
}
}
----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The
} Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
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}
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}
} We found that the red (typically rhodamine) images
} taken with our
} Zeiss Axiocam MRc are fuzzy. We looked at the blue
} and found this a
} problem too, but since in fluorescence people
} typically use the blue
} just as a marker for nucleii rather than as stain
} for fine structure,
} they didn't notice this problem. (Illustrations at
} http://www.aecom.yu.edu/aif/temp/axiocamproblem/ ).
}
} This made us worry about the sharpness of images of
} histological
} stained samples, such as H&E.
}
} First we checked that this isn't a problem due to
} the fluorescent
} filter sets. To do this, we filtered the light to
} be red, green or
} blue at the halogen lamp using RGB filters removed
} from an old 35mm
} film recorder and imaged the sample with no filters
} in the light path
} between the sample and the camera. The result was
} that the red and
} blue images were very fuzzy.
}
} Shooting in the b/w mode solves this problem, but
} then the images are
} not color. Also, this may solve the problem for
} fluorescence, but
} what about brightfield or other techniques that
} involve colors?
}
} Can anyone explain why this happens and how to fix
} it? Is this a
} problem with our sytem uniquely or is this a problem
} with the Axiocam
} in general?
}
} Thanks!
}
} -Michael
}
____________________________________________________________________________
} Michael Cammer Analytical Imaging Facility
} Albert Einstein Coll. of Med.
} URL: http://www.aecom.yu.edu/aif/
}
}
} ==============================Original
} Headers==============================
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From: dljones-at-bestweb.net
Date: Fri, 26 Jan 2007 08:21:46 -0600
Subject: [Microscopy] Re: Nitrogen free epoxy?

Contents Retrieved from Microscopy Listserver Archives
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John,

I really know little of what you are doing, but I haven't seen any
answers to your question so I thought I'd throw something back at you.

Can you use something other than epoxy? For example a polyester mounting
material?

The polyester poly(ethyene terephthalate) doesn't have any nitrogen in it,
for example. I think several other forms of polyesters also do not contain
nitrogen, but I am no expert in that field.

There is a commercial product from Extec that is a polyester mount
material. I don't know if it's nitrogen free, but you could ask them for
the MSDS of the product and see. A link to their web page where they have
their cold mounting supplies is:

http://www.extec.com/coldmounting.htm

Disclaimer: I have no commercial interest in this company or it's
products.

I do use their polyester mounting material when I have samples I need to
look at close to the edge with the mount and I don't want all the added
elements usually found in epoxies. But I've not yet been concerned with
nitrogen as one of those elements...

Good luck, and I would like to know what you do end up finding if you
wouldn't mind posting your results...

dj

On Thu, 25 Jan 2007, donovan-at-uoregon.edu wrote:

}
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} All,
} Can anyone tell me of a source for a nitrogen free epoxy? I realize
} that may be a contradiction in terms, but I need to find a mounting
} media that can hold a water soluble polymer coated wire for cross
} sectioning for subsequent nitrogen analysis by EPMA.
}
} Thanks!
} john
}
} John J. Donovan donovan-at-uoregon.edu
} University of Oregon (541) 346-4632 (office)
} 1260 Franklin Blvd (541) 346-4655 (probe)
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From: hikonishi-at-gmail.com
Date: Fri, 26 Jan 2007 12:35:31 -0600
Subject: [Microscopy] Ultramicrotomy testing

Contents Retrieved from Microscopy Listserver Archives
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Hello:

We are planning to buy an ultramicrotome at a high probability, and we would
like to microtome some materials for testing. If some labs in the
manufacturers can help us, please contact me by email. Our materials are
metal and perhaps ceramics.



Hiromi Konishi, Ph.D.
Laboratory Manager
The S.W. Bailey X-ray Diffraction Laboratory
Room A353 Weeks Hall
Voice: (608) 262-9784, (608) 262-0915
Fax: (608) 262-0693
Department of Geology and Geophysics
University of Wisconsin-Madison
1215 W Dayton St. Madison WI 53706


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From: Jessica.Wagner-at-childrens.harvard.edu
Date: Fri, 26 Jan 2007 14:24:29 -0600
Subject: [Microscopy] imaging the occular view through another port?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hello list. I have a question that's just for fun. I'm using a Nikon TE-2000 wth all 4 ports and a beam splitter that can direct light 50/50 split between two ports. Is there a way to set up a camera to image not a sample but specifically the image that I'm seeing? I have a minor defect in my eye, a wrinkle caused by slight detachment of the vitreous (I think due to having LASIK done). When I look through the scope at a bright field, I can see an image of the wrinkle. I'm curious to know if I can capture it, but my guess is that the image only exists in the occulars? Maybe I could somehow use a dichroic mirror?

Thanks,
Jessica



==============================Original Headers==============================
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From: jquinn-at-www.matscieng.sunysb.edu
Date: Fri, 26 Jan 2007 14:28:55 -0600
Subject: [Microscopy] re: SEM for 12" or 300mm sample

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Folks -

I am looking for a SEM with EDS to check out
a 12" or 300mm Si sample/disk/target.

We would like to scan around the
surface of the silicon target.

Please respond "off-listserv".

kind regards,

Jim






==============================Original Headers==============================
12, 13 -- From jquinn-at-www.matscieng.sunysb.edu Fri Jan 26 14:28:54 2007
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12, 13 -- Date: Fri, 26 Jan 2007 15:23:46 -0500
12, 13 -- From: Jim Quinn {jquinn-at-www.matscieng.sunysb.edu}
12, 13 -- Message-Id: {200701262023.l0QKNkj14934-at-www.matscieng.sunysb.edu}
12, 13 -- To: Microscopy-at-microscopy.com
12, 13 -- Subject: re: SEM for 12" or 300mm sample
12, 13 -- Cc: waldvogel-at-prodigy.net
==============================End of - Headers==============================




From: bozzola-at-siu.edu
Date: Fri, 26 Jan 2007 15:01:39 -0600
Subject: [Microscopy] Re: imaging the occular view through another port?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hello,

I have experienced this same phenomenon with "floaters" in my eyes.
Under the right conditions, I can see the floaters beautifully and
sometimes even the corneal surface. As explained to me by an
ophthomologist, this is due to the projection of a shadow (of the
objects) onto the retina. Therefore, you would not be able to "go the
other way" and see the image in a viewing port. I believe that the
only way to image this would be with a slit lamp (as in an
ophthomological examination).

Maybe others on the list would care to comment since I am certainly
no expert but speaking only from personal experience.

JB

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==============================Original Headers==============================
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From: tivol-at-caltech.edu
Date: Fri, 26 Jan 2007 15:39:41 -0600
Subject: [Microscopy] Re: imaging the occular view through another port?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


On Jan 26, 2007, at 12:24 PM, Jessica.Wagner-at-childrens.harvard.edu
wrote:

} } Hello list. I have a question that's just for fun. I'm using a
} } Nikon TE-2000 wth all 4 ports and a beam splitter that can direct
} } light 50/50 split between two ports. Is there a way to set up a
} } camera to image not a sample but specifically the image that I'm
} } seeing? I have a minor defect in my eye, a wrinkle caused by slight
} } detachment of the vitreous (I think due to having LASIK done). When
} } I look through the scope at a bright field, I can see an image of the
} } wrinkle. I'm curious to know if I can capture it, but my guess is
} } that the image only exists in the occulars? Maybe I could somehow
} } use a dichroic mirror?
} }
} I have experienced this same phenomenon with "floaters" in my eyes.
} Under the right conditions, I can see the floaters beautifully and
} sometimes even the corneal surface. As explained to me by an
} ophthomologist, this is due to the projection of a shadow (of the
} objects) onto the retina. Therefore, you would not be able to "go the
} other way" and see the image in a viewing port. I believe that the
} only way to image this would be with a slit lamp (as in an
} ophthomological examination).
}
} Maybe others on the list would care to comment since I am certainly
} no expert but speaking only from personal experience.

Dear Jessica and John,
The image "that I'm seeing" can be thought of as the image that a user
with normal sight would see transformed by the effect of the defect, so
although it is easy to see the "normal" image, and as John suggests,
you could see the defect with a slit lamp, you could not see the
perturbation caused by the defect (although there are ways in theory to
calculate it from the structure of the defect). Of course, you could
look at a grid or some such test object and draw what you see, so the
distortions noted in the grid will tell you what distortions to expect
in a more complex image. You could even look at the grid separately
with each eye and compare the image from the undamaged eye to that from
the eye with the defect, and, if your brain has not already adapted to
seeing the distorted image, looking at a grid that has been distorted
in one eye could be interpreted by your brain as a 3D image of the grid
that appears to be closer to you in some places than in others, like
the effect of viewing stereo images, but much more subtle.
Yours,
Bill Tivol, PhD
EM Scientist and Manager
Cryo-Electron Microscopy Facility
Broad Center, Mail Code 114-96
California Institute of Technology
Pasadena CA 91125
(626) 395-8833
tivol-at-caltech.edu


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From: ahlst007-at-umn.edu
Date: Fri, 26 Jan 2007 15:46:29 -0600
Subject: [Microscopy] Re: Nitrogen free epoxy?

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John,

Unless you will be looking for trace amounts of
nitrogen in your polymer, you might be able to
get away with using one of the Epon epoxy
substitutes, like EMbed 812.

Of its four components, only the accelerator
DMP-30 has any N, 3 atoms per molecule, only a
small amount is used in the mix, about 1.25%
v/v, and the mass fraction of N in that 1.25% is
even less. Another accelerator that can be used
with EMbed812, BDMA, has only one N atom per
molecule.

As it happened, I was scheduled to do some
SEM-EDS this morning, so I took a look at a
sample of an EMbed 812 block, hardened with
DMP-30. I could see no detectable N peak in the
spectra. I'll send you a JPG of a spectra off-List.

As you will be doing EPMA, your sensitivity for
N maybe be greater. But you could just check
some epoxy resins to see if there is enough N
present to be a problem for your polymer work.

But of course, there is nothing sweeter than a
nice, clean elemental analysis!

Good luck in your search for an N-free medium.

Gib

Gilbert (Gib) Ahlstrand, Electron Microscopist,
Imaging Center, University of Minnesota
123 Snyder Hall
St. Paul, MN 55108
http://www.cbs.umn.edu/ic

donovan-at-uoregon.edu wrote:
} ----------------------------------------------------------------------------
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} All,
} Can anyone tell me of a source for a nitrogen free epoxy? I realize
} that may be a contradiction in terms, but I need to find a mounting
} media that can hold a water soluble polymer coated wire for cross
} sectioning for subsequent nitrogen analysis by EPMA.
}
} Thanks!
} john
}
} John J. Donovan donovan-at-uoregon.edu
} University of Oregon (541) 346-4632 (office)
} 1260 Franklin Blvd (541) 346-4655 (probe)
} Eugene, OR (541) 346-4778 (SEM)
} 97403-1272 (541) 346-4692 (FAX)

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From: rcommon-at-msu.edu
Date: Fri, 26 Jan 2007 15:58:38 -0600
Subject: [Microscopy] imaging the occular view through another port?

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You probably would not like one possible approach to solving this imaging
problem. I am thinking of Kevin Kline's solution to a similar problem in
the movie "Wild Wild West".

Ralph Common


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From: CGoldsmith-at-cdc.gov
Date: Fri, 26 Jan 2007 17:33:12 -0600
Subject: [Microscopy] viaWWW: Southeastern Microscopy Society annual meetin

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Email: CGoldsmith-at-cdc.gov
Name: Cynthia Goldsmith

Organization: Southeastern Microscopy Society

Title-Subject: [Filtered] Southeastern Microscopy Society annual meetin

Question: The 2007 annual meeting for the Southeastern Microscopy Society (SEMS) will be held April 11-13 in historic Decatur, Georgia, a suburb of Atlanta. We have an exciting program planned, including pre-meeting workshops on Bio-TEM Imaging, QuantomiX Wet SEM, Cryoultramicrotomy, and Deconvolution Microscopy. In addition, invited speakers will present information on such topics as the examination of the Gospel of Judas, World Trade Center dust, and macrophage cholesterol metabolism.

Deadlines for registration and abstract submission are February 23, 2007. There is additional meeting information available at the SEMS website at www.southeasternmicroscopy.org.


Cynthia S. Goldsmith
Secretary, Southeastern Microscopy Society (SEMS)



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From: TindallR-at-missouri.edu
Date: Mon, 29 Jan 2007 09:38:24 -0600
Subject: [Microscopy] TEM:Maleic vs. maleate vs. malic acid buffers

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Dear Listers,

I guess this is my month for desperate listserve queries, so here I go
again.

We are trying to follow a not-very-detailed protocol for brain tissue
which utilizes maleic acid buffer for wash steps before and after the
"uranyl acetate colorization solution". One would presume that this
refers to en bloc staining also using this buffer, but no details are
given as to molarity, buffer composition, etc. We have no experience
with this buffer, although I've heard of it, and are having a terrible
time finding good references regarding its use and formulation.

My research in our reference library and online shows many instances of
en bloc staining using maleic acid buffer, tris-maleate buffer, or
simply maleic acid, so my questions are:

1) Are these terms pretty much interchangeable (i.e., is tris-maleate
buffer just maleic acid buffer with the addition of tris)?

2) Do you all have any preferred formulations for this buffer in the UA
step? Most of the refs I've seen use 0.05M - 0.2M buffer with 0.5-1%
UA.

3) Is this buffer pretty much restricted to en bloc staining, at least
for use in TEM studies?

4) What are the advantages of this buffer over aqueous en bloc staining
with UA?

Thanks to all.

Cheers,
Randy

Randy Tindall
Senior EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W125 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu
On-line calendar:
http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=
Week&NavType=Both&Type=TimePlan


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From: mconstan-at-princeton.edu
Date: Mon, 29 Jan 2007 10:04:18 -0600
Subject: [Microscopy] AskAMicroscopist: SEM beam penetration

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Email: mconstan-at-princeton.edu
Name: Michael

Organization: Princeton University

Education: Undergraduate College

Location: Princeton, NJ, USA

Title: SEM beam penetration

Question: Hi. I'm an undergrad working in a lab and I would like to
use SEM to view gold nanoparticles embedded in agarose gel. About
how far could the SEM beam penetrate into 1% agarose (I'm guessing I
would have to let the water in the gel evaporate)? Would it be
better to use SE or BSE? What kV should I try? Thanks for any help
you can give me!

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From: phillipst-at-missouri.edu
Date: Mon, 29 Jan 2007 10:17:23 -0600
Subject: [Microscopy] Re: Maleate buffer

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Here is what we use with success:

Sodium Maleate (Maleic acid, monosodium salt)
MW = 138.1
50 mM = 691 mg/ 100 mls

Assume uranyl acetate stock is 5%. Dilute 1 part + 9 parts of 50 mM sodium
maleate

En bloc staining protocol:

After osmium, rinse tissue 3x 5 min each in dH2O

rinse tissue 3x 5 min each in 50 mM sodium maleate, pH 5.2

incubate tissue for 1 hr to overnight in 0.5% - 1.0% uranyl acetate in 50
mM sodium maleate, pH 5.2 (for overnight, probably should use 0.5% UA, for
1 hr probably should use 1% UA).

rinse 3x dH2O for 10 min

50% ethanol 10 min

70% ethanol 10 min

95% ethanol 10 min

3x 100% ethanol 10 min

start resin infiltration



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From: john.mardinly-at-intel.com
Date: Mon, 29 Jan 2007 11:51:40 -0600
Subject: [Microscopy] imaging the occular view through another port?

Contents Retrieved from Microscopy Listserver Archives
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What kind of detail do you need? That will probably determine your
conditions.

Understand that the beam will start scattering and interacting once it
encounters the first surface which will be agarose. Secondary electrons
are limited to escaping from the first few nm of surface, so even if
they are generated at some depth, they will not escape to be detected. I
expect you would see primarily the agarose surface.

There is also the question of imaging the agarose at all. I presume it
would charge without coating and would need to be examined in variable
pressure or environmental mode. That would eliminate the choice of SE
unless you have a gaseous SE detector, in which case the previous
comments still apply.

BSE could give you some information from some depth below the surface.
It will still be difficult and there will be some scattering.

Also, what happens to the structure of the agarose under vacuum? I
suppose there will be appreciable loss of moisture, so what you see may
not be anything like what you had. I would check the mass loss as a
result of exposure to vacuum. It might be too extreme.

I would probably start at 20kV for voltage and set the current to give a
decent BSE image. You will probably have to experiment from there.

Warren Straszheim

-----Original Message-----
X-from: mconstan-at-princeton.edu [mailto:mconstan-at-princeton.edu]
Sent: Monday, January 29, 2007 10:05 AM
To: wesaia-at-iastate.edu

Jessica;
Some time ago, there was a thread about LASIK and microscopists.
I do not recall any of the reports mentioning this sort of post-surgical
vision difficulty. Is this perhaps a subject that should be re-visited?

John Mardinly
Intel

Disclaimer: The opinions of this author do not represent the opinions of
Intel Corporation.

-----Original Message-----
X-from: Jessica.Wagner-at-childrens.harvard.edu
[mailto:Jessica.Wagner-at-childrens.harvard.edu]
Sent: Friday, January 26, 2007 12:25 PM
To: Mardinly, John

Hello list. I have a question that's just for fun. I'm using a Nikon
TE-2000 wth all 4 ports and a beam splitter that can direct light 50/50
split between two ports. Is there a way to set up a camera to image not
a sample but specifically the image that I'm seeing? I have a minor
defect in my eye, a wrinkle caused by slight detachment of the vitreous
(I think due to having LASIK done). When I look through the scope at a
bright field, I can see an image of the wrinkle. I'm curious to know if
I can capture it, but my guess is that the image only exists in the
occulars? Maybe I could somehow use a dichroic mirror?

Thanks,
Jessica



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From: VGriffiths-at-mtech.edu
Date: Mon, 29 Jan 2007 12:00:34 -0600
Subject: [Microscopy] Epoxy free mounting material

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Another possibility is to use a mounting material from long,long ago.
This is Wood's metal, a quaternary eutectic of Bi/50%, Pb/25%, Sn12.5%,
Cd12.5%. It's used for sprinkler plugs in automatic systems.
If your polymer can stand 70'C, (melting point of the alloy) it.s a good
way to go since you be sure there's no nitrogen in it. The alloy is
fairly soft and it wet's well.

Vern Griffiths
Montana Tech


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From: gradice-at-richmond.edu
Date: Mon, 29 Jan 2007 14:05:41 -0600
Subject: [Microscopy] real microscopes vs lens equations

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

I'm teaching a bioimaging course for undergraduates. We are now
discussing the lens equations that relate object and image distance
to focal length:

1/p + 1/q = 1/f

and magnification:

M = q/p

where p is the object distance and q is the image distance.

We've demonstrated these relationships using lenses on an optical
bench, and now I want to transfer to real microscopes.

The students have access to Nikon Alphaphot microscopes with a fixed
tube length of 160 mm, which I understand is measured from the
nosepiece to the eyepiece mount. The eyepiece mount is approximately
the same as the primary image plane on these 'scopes. So does that
mean that the image distance for each lens should be 160 mm plus the
distance to the middle of the objective lens? Or is it more
complicated than that, because the lens equations don't work out very
well using those values for image distance.

For example, the 10x objective should then have an image distance of
about 160 + 38 mm = 198 mm. Which would then predict an object
distance of 19.8 mm and a focal length of 18 mm. All of that makes
sense since the object should be just beyond the front focal point of
the objective to make a real inverted image at the primary image plane.

But in practice, the working distance of this lens is 6.1 mm, which
puts the actual object distance at around 6.3 mm, well inside the
presumed focal point, and puts the image distance at around 63 mm for
a 10 magnification

Something doesn't add up. What am I missing?





Gary P. Radice gradice-at-richmond.edu
Department of Biology 804-289-8107 (voice)
University of Richmond 804-289-8233 (FAX)
Richmond VA 23173 http://www.richmond.edu/~gradice



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From: Jessica.Wagner-at-childrens.harvard.edu
Date: Mon, 29 Jan 2007 16:18:54 -0600
Subject: [Microscopy] imaging the occular view through another port?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

John,

I missed that thread, but I'll be happy to add my two cents now.
Assuming the PVD (posterior vitreous detachment) doesn't get any worse,
for me the benefits of LASIK still outweigh this minor negative. The
'floater' that I see is an annoyance, but it doesn't truly inhibit my
vision through the scope (or outside the scope for that matter).

But I had LASIK only a year ago, and was never warned PVD could be a
complication, so this says to me that there is still much unknown about
the procedure and it's results. Unfortunately, complications of laser
eye surgeries really aren't tracked all that well; doctors are not
required to report them, unless they are related to a device, but even
then, many doctors are not aware of FDA regulations about device event
reporting or how/to whom they should report adverse events.

Here is an article about PVD's and LASIK:
http://www.springerlink.com/content/j3100858467pu5k1/

And thanks to everyone who offered imaging advice!

Jessica


-----Original Message-----
X-from: john.mardinly-at-intel.com [mailto:john.mardinly-at-intel.com]
Sent: Monday, January 29, 2007 12:56 PM
To: Wagner, Jessica

Jessica;
Some time ago, there was a thread about LASIK and microscopists.
I do not recall any of the reports mentioning this sort of post-surgical
vision difficulty. Is this perhaps a subject that should be re-visited?

John Mardinly
Intel

Disclaimer: The opinions of this author do not represent the opinions of
Intel Corporation.

-----Original Message-----
X-from: Jessica.Wagner-at-childrens.harvard.edu
[mailto:Jessica.Wagner-at-childrens.harvard.edu]
Sent: Friday, January 26, 2007 12:25 PM
To: Mardinly, John

Hello list. I have a question that's just for fun. I'm using a Nikon
TE-2000 wth all 4 ports and a beam splitter that can direct light 50/50
split between two ports. Is there a way to set up a camera to image not
a sample but specifically the image that I'm seeing? I have a minor
defect in my eye, a wrinkle caused by slight detachment of the vitreous
(I think due to having LASIK done). When I look through the scope at a
bright field, I can see an image of the wrinkle. I'm curious to know if
I can capture it, but my guess is that the image only exists in the
occulars? Maybe I could somehow use a dichroic mirror?

Thanks,
Jessica



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From: sbarlow-at-sunstroke.sdsu.edu
Date: Mon, 29 Jan 2007 20:07:58 -0600
Subject: [Microscopy] negative staining viruses in cesium chloride

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

One of my users is trying to visualize virus particles by negative
stain, normally a routine procedure here. However, her sample is
straight off a cesium chloride purification. Will the CsCl solution
inhibit binding of the virus particles to coated grids, or should she
be able to use standard neg. staining procedures to see her
particles. She didn't see any particles in her first attempts.
While trying to calculate virus concentrations to determine if her
sample is concentrated enough, I wondered if the CsCl will inhibit
virus sticking to the grids--I have no experience with virus samples
that have not been purified into some other buffer system.

Anyone have a protocol we can use for such a sample, or does she need
to get rid of the CsCl before she tries to do any negative stain
imaging.

Your advice is appreciated.

Steve
--
Dr. Steven Barlow
EM Facility/Biology Dept.
San Diego State University
5500 Campanile Drive
San Diego CA 92182-4614
phone: (619) 594-4523
fax: (619) 594-5676

email: sbarlow-at-sunstroke.sdsu.edu
http://www.sci.sdsu.edu/emfacility

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From: skuo-at-jhu.edu
Date: Mon, 29 Jan 2007 21:06:16 -0600
Subject: [Microscopy] viaWWW: Job Opening: Microscopist

Contents Retrieved from Microscopy Listserver Archives
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Email: skuo-at-jhu.edu
Name: Scot Kuo

Organization: Johns Hopkins University

Title-Subject: [Filtered] Job Opening: Microscopist, try#2

Question: ...Try#2: Prior attempt lost line-breaks...
{br /}

With light, fluorescence or confocal microscopy experience,
a microscopy specialist is sought to provide user support
in Johns Hopkins School of Medicine Microscope Facility
(http://www.hopkinsmedicine.org/micfac/). This facility
provides light, fluorescence and electron microscopy
services to more than 250 users throughout Johns Hopkins.
As part of a team with other full-time staff, the
candidateís main duties are to help assist in training and
supervising new users, as well as help users troubleshoot
experiments. Because of the diversity of equipment within
the facility, we will provide initial training as necessary.
Consequently, the candidate must display resourceful
independence, a willingness to learn and have strong
analytical and computer skills.
{br /}

Please refer to JHUjobs (http://jobs.jhu.edu/) posting #26703
(https://hrnt.jhu.edu/jhujobs/job_view.cfm?view_req_id=26703)
for details about minimal qualifications and application
process. More qualified individuals will be considered for
appropriate compensation.
{br /}

===============================================================================
{br /}
Scot C. Kuo, skuo-at-jhu.edu, www.jhu.edu/cmml/; www.hopkinsmedicine.org/micfac/
{br /}
Director, Imaging for Basic Biomedical Sciences, (410)955-4536
{br /}
Wood Basic Science Bldg, Room G10, 725 N Wolfe St, Baltimore MD 21205
{br /}
Associate Professor, Biomedical Engineering & Cell Biology
{br /}
===============================================================================
{br /}


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From: swtkeller-at-yahoo.com
Date: Mon, 29 Jan 2007 21:06:45 -0600
Subject: [Microscopy] viaWWW: TEM-Defect density Threshold

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Email: swtkeller-at-yahoo.com
Name: Sandra Keller

Organization: TA/SICCO

Title-Subject: [Filtered] TEM-Defect density Threshold

Question: Hi All:
I was asked by a colleague of mine to inquire from the Listserver: What is the approximate defect density in silicon, one can easily visualize using a 120-200 KV TEM (plane view and cross-sectional view)?

Thanks in advance for being such a valuable resource,
Sandra Keller

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From: mconstan-at-princeton.edu
Date: Mon, 29 Jan 2007 21:08:55 -0600
Subject: [Microscopy] AskAMicroscopist SEM beam penetration

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Email: mconstan-at-princeton.edu
Name: Michael

Organization: Princeton University

Education: Undergraduate College

Location: Princeton, NJ, USA

Title: SEM beam penetration

Question: Hi. I'm an undergrad working in a lab and I would like to use SEM to view gold nanoparticles embedded in agarose gel. About how far could the SEM beam penetrate into 1% agarose (I'm guessing I would have to let the water in the gel evaporate)? Would it be better to use SE or BSE? What kV should I try? Thanks for any help you can give me!

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From: richard.beanland-at-bookham.com
Date: Tue, 30 Jan 2007 03:10:23 -0600
Subject: [Microscopy] viaWWW: TEM-Defect density Threshold

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Sandra,
It's an easy calculation to do if you make some rough
assumptions about the amount of thin material in your TEM specimen and
the visibility of defects. For a standard cross section which has
roughly 100um of electron transparent material away from the edges of
the hole and is on average 1um thick, you have 0.01 x 0.0001 = 0.000001
cm^2 of the original wafer surface in your cross-section specimen. So
if you see just one defect you have a density of 10^6 defects cm^-2. If
your defects are only visible in thin material then revise the number
upwards. If you have a FIB section (say 30um wide and 0.3 um thick) you
need over 10^8 defects cm^-2 to catch one in your specimen.

For a plan view specimen which has the same 100um electron transparent
area and average thickness of 1um, say around a hole 50um wide then the
amount of the original wafer surface you can see is
pi*(150um)^2-pi*(50um)^2 = 0.0006 cm^2. So if you see one defect you
have a density of about 1600 defects cm^-2.

Having said that I know from experience it can take some time to find
defects in plan view specimens even when the density as high as 10^5
defects cm^-2, simply because the specimen is always bent and you have
to tilt the specimen through a diffraction condition to see them.

Defect etching is very useful for seeing low densities of defects if
processing allows. You can even do a light defect etch and then make a
TEM specimen - in which case you will find that not all of the defects
actually produce an etch pit, which just goes to show that there is no
perfect technique for measuring defect densities.

All the best

Richard



________________________________________
Richard Beanland
Materials Analysis
Bookham
Caswell
Towcester
Northants
NN12 8EQ
United Kingdom
Tel. +44 1327 356362
Fax. +44 1327 356775
http://www.bookham.com
________________________________________


-----Original Message-----
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Sent: 30 January 2007 03:08
To: Richard Beanland

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://microscopy.com/MLFormMail.html
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Email: swtkeller-at-yahoo.com
Name: Sandra Keller

Organization: TA/SICCO

Title-Subject: [Filtered] TEM-Defect density Threshold

Question: Hi All:
I was asked by a colleague of mine to inquire from the Listserver: What
is the approximate defect density in silicon, one can easily visualize
using a 120-200 KV TEM (plane view and cross-sectional view)?

Thanks in advance for being such a valuable resource,
Sandra Keller

------------------------------------------------------------------------
---

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From: nizets2-at-yahoo.com
Date: Tue, 30 Jan 2007 04:07:41 -0600
Subject: [Microscopy] basic question ultramicrotopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi

The simplest solution to your problem is to down load one of the basic Monte
Carlo simulations and consider your material to be Carbon. The Joy-Nockolds
version is on our web site under hints and tips.

Depending upon the microscope that you are using, the magnification, the kV
and the working distance, you could find the so called "secondary electron
signal" (everhart-Thornley detector) carries sufficient backscattered
information to provide the data that you require. Be aware of the fall off
in resolution when using dedicated backscatter as the performance may fall
below that required to visualise your particles. You should also consider
that depending on the microscope and your own skill the size of particles
may be too small to visualise?

You should try the complete range of kV as the information will always be
better at one particular kV. It is impossible perhaps foolish to try and
guess what that may be. You may need higher kVs for penetration but too
much will fog the image with unimportant sub surface data, whilst too low a
kV may not provide the resolution that you require. Microscopists are
scientists we should experiment!

Good luck

Steve Chapman
Protrain for EM training & consultancy world wide
www.emcourses.com
Tel +44 1280 816512 Fax +44 1280 814007
Mobile +44 7711 606967


----- Original Message -----
X-from: {mconstan-at-princeton.edu}
To: {protrain-at-emcourses.com}
Sent: Tuesday, January 30, 2007 3:10 AM

Hello colleagues,

I have (again) a pretty basic, non-life-threatening
question about ultramicrotomy (with a Leica EM UC6 in
my case).
When I am cutting ultrathin sections it may happen I
have to pause for a while (because I have to sign
autographs, or to pick up sections from water). After
the pause when I start the cutting cycle again, it
very often does not cut immediately but I have to cut
500nm or more to touch the block again.
I wondered if this was a sort of automatic protection
from the machine, which retracts a little after some
time of inactivity to avoid damage to the knife, or if
this a the normal consequence of an effect of physics.
Any comment on this?

Stephane




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From: David.Patton-at-uwe.ac.uk
Date: Tue, 30 Jan 2007 05:57:17 -0600
Subject: [Microscopy] basic question ultramicrotopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Stephane, you are lucky - no accidental 1000nm sections! When I stop to
sign autographs or explain to new users what is going on, the block in
my Ultracut E sneaks forward and I demonstrate how to ruin the knife
with a thick section. I accept this is probably due to operator error
although an expert suggested that the block is compressed during
sectioning and if left for a while will expand forward.

Dave

-----Original Message-----
X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com]
Sent: 30 January 2007 10:12
To: David Patton

Hello colleagues,

I have (again) a pretty basic, non-life-threatening
question about ultramicrotomy (with a Leica EM UC6 in
my case).
When I am cutting ultrathin sections it may happen I
have to pause for a while (because I have to sign
autographs, or to pick up sections from water). After
the pause when I start the cutting cycle again, it
very often does not cut immediately but I have to cut
500nm or more to touch the block again.
I wondered if this was a sort of automatic protection
from the machine, which retracts a little after some
time of inactivity to avoid damage to the knife, or if
this a the normal consequence of an effect of physics.
Any comment on this?

Stephane




________________________________________________________________________
____________
It's here! Your new message!
Get new email alerts with the free Yahoo! Toolbar.
http://tools.search.yahoo.com/toolbar/features/mail/

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18, 34 -- Subject: RE: [Microscopy] basic question ultramicrotopy
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From: TindallR-at-missouri.edu
Date: Tue, 30 Jan 2007 08:19:52 -0600
Subject: [Microscopy] basic question ultramicrotopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Stephane,

I have the opposite problem, maybe because I'm usually asking for
autographs, instead of signing them. If I take a break, I learned the
hard way that the first swipe of the block over the knife will usually
cut off a section about as thick as a slice of bread. I've often
wondered why----especially after one time losing a single layer of cells
which floated off into the sunset. (I had spares, so no worries, mate.)
We have Leica UCTs.

Randy

Randy Tindall
Senior EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W125 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu
On-line calendar:
http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=
Week&NavType=Both&Type=TimePlan


-----Original Message-----
X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com]
Sent: Tuesday, January 30, 2007 4:08 AM
To: Tindall, Randy D.

Hello colleagues,

I have (again) a pretty basic, non-life-threatening question about
ultramicrotomy (with a Leica EM UC6 in my case).
When I am cutting ultrathin sections it may happen I have to pause for a
while (because I have to sign autographs, or to pick up sections from
water). After the pause when I start the cutting cycle again, it very
often does not cut immediately but I have to cut 500nm or more to touch
the block again.
I wondered if this was a sort of automatic protection from the machine,
which retracts a little after some time of inactivity to avoid damage to
the knife, or if this a the normal consequence of an effect of physics.
Any comment on this?

Stephane




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From: Michal.Jarnik-at-fccc.edu
Date: Tue, 30 Jan 2007 08:40:45 -0600
Subject: [Microscopy] Re: basic question ultramicrotopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

I don't really think this is an operator error, it does happen all the
time, standard or cryo, I am not sure why. After breaking a couple of
cryoblocks (not ruining any diamond on plastic, so far), I always go
200-400 nm back (using coarse advance) when pausing for section
retrieval. Usually I have to wait for 3-4 cycles for the microtome to
start cutting again, but it solves the problem.

Michal

nizets2-at-yahoo.com wrote:
} ----------------------------------------------------------------------------
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} ----------------------------------------------------------------------------
}
} Hello colleagues,
}
} I have (again) a pretty basic, non-life-threatening
} question about ultramicrotomy (with a Leica EM UC6 in
} my case).
} When I am cutting ultrathin sections it may happen I
} have to pause for a while (because I have to sign
} autographs, or to pick up sections from water). After
} the pause when I start the cutting cycle again, it
} very often does not cut immediately but I have to cut
} 500nm or more to touch the block again.
} I wondered if this was a sort of automatic protection
} from the machine, which retracts a little after some
} time of inactivity to avoid damage to the knife, or if
} this a the normal consequence of an effect of physics.
} Any comment on this?
}
} Stephane
}
}
}
}
} ____________________________________________________________________________________
} It's here! Your new message!
} Get new email alerts with the free Yahoo! Toolbar.
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From: thoward-at-unm.edu
Date: Tue, 30 Jan 2007 09:01:49 -0600
Subject: [Microscopy] Re: basic question ultramicrotopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

I must be doing something very wrong - I'm only called
away from the microtome to be informed of malfunctioning
microscopes/gel boxes/processors/etc. - never to sign
autographs.
When I return from the crisis, sometimes the block is
further forward, sometimes back - I always retract the
knife out of paranoia. I think the block moving away from
the knife is due to thermal effects; you may generate
enough friction during cutting to cause thermal expansion
of the block, which then shrinks back when you stop
cutting. At least, that is what I was told when I was
learning to cut. I think it moves forward out of pure
contrariness. I know on the old thermal advance microtomes
you were pretty much guaranteed a trashed block/knife if
you didn't retract when re-starting...there were all kinds
of odd behaviors associated with those machines.

Tamara


On Tue, 30 Jan 2007 04:08:34 -0600
nizets2-at-yahoo.com wrote:
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy
} Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help
} http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Hello colleagues,
}
} I have (again) a pretty basic, non-life-threatening
} question about ultramicrotomy (with a Leica EM UC6 in
} my case).
} When I am cutting ultrathin sections it may happen I
} have to pause for a while (because I have to sign
} autographs, or to pick up sections from water). After
} the pause when I start the cutting cycle again, it
} very often does not cut immediately but I have to cut
} 500nm or more to touch the block again.
} I wondered if this was a sort of automatic protection
} from the machine, which retracts a little after some
} time of inactivity to avoid damage to the knife, or if
} this a the normal consequence of an effect of physics.
} Any comment on this?
}
} Stephane
}
}
}
}
} ____________________________________________________________________________________
} It's here! Your new message!
} Get new email alerts with the free Yahoo! Toolbar.
} http://tools.search.yahoo.com/toolbar/features/mail/
}
} ==============================Original
} Headers==============================
} 6, 19 -- From nizets2-at-yahoo.com Tue Jan 30 04:07:40 2007
} 6, 19 -- Received: from web37407.mail.mud.yahoo.com
} (web37407.mail.mud.yahoo.com [209.191.91.139])
} 6, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8)



From: David.Patton-at-uwe.ac.uk
Date: Tue, 30 Jan 2007 09:15:00 -0600
Subject: [Microscopy] Re: basic question ultramicrotopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

On a related theme. Without pausing to sign autographs etc has anyone
demonstrated that turning the "wheel" only moves the block up and down,
only to have puzzled students say "But it is cutting sections."?

Dave

-----Original Message-----
X-from: thoward-at-unm.edu [mailto:thoward-at-unm.edu]
Sent: 30 January 2007 15:06
To: David Patton

I must be doing something very wrong - I'm only called
away from the microtome to be informed of malfunctioning
microscopes/gel boxes/processors/etc. - never to sign
autographs.
When I return from the crisis, sometimes the block is
further forward, sometimes back - I always retract the
knife out of paranoia. I think the block moving away from
the knife is due to thermal effects; you may generate
enough friction during cutting to cause thermal expansion
of the block, which then shrinks back when you stop
cutting. At least, that is what I was told when I was
learning to cut. I think it moves forward out of pure
contrariness. I know on the old thermal advance microtomes
you were pretty much guaranteed a trashed block/knife if
you didn't retract when re-starting...there were all kinds
of odd behaviors associated with those machines.

Tamara


On Tue, 30 Jan 2007 04:08:34 -0600
nizets2-at-yahoo.com wrote:
}
}
}
}
------------------------------------------------------------------------
----
} The Microscopy ListServer -- CoSponsor: The Microscopy
} Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help
} http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
------------------------------------------------------------------------
----
}
} Hello colleagues,
}
} I have (again) a pretty basic, non-life-threatening
} question about ultramicrotomy (with a Leica EM UC6 in
} my case).
} When I am cutting ultrathin sections it may happen I
} have to pause for a while (because I have to sign
} autographs, or to pick up sections from water). After
} the pause when I start the cutting cycle again, it
} very often does not cut immediately but I have to cut
} 500nm or more to touch the block again.
} I wondered if this was a sort of automatic protection
} from the machine, which retracts a little after some
} time of inactivity to avoid damage to the knife, or if
} this a the normal consequence of an effect of physics.
} Any comment on this?
}
} Stephane
}
}
}
}
}
________________________________________________________________________
____________
} It's here! Your new message!
} Get new email alerts with the free Yahoo! Toolbar.
} http://tools.search.yahoo.com/toolbar/features/mail/
}
} ==============================Original
} Headers==============================
} 6, 19 -- From nizets2-at-yahoo.com Tue Jan 30 04:07:40 2007
} 6, 19 -- Received: from web37407.mail.mud.yahoo.com
} (web37407.mail.mud.yahoo.com [209.191.91.139])
} 6, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8)



From: rblyston-at-trinity.edu
Date: Tue, 30 Jan 2007 09:31:11 -0600
Subject: [Microscopy] Differential Ultramicrotomy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

To all:

The chucked block in the unattended ultramicrotome obeys the
Hehesenberg Uncertainty Principle. As I trust you remember the
position of a chucked block whose momentary momentum is zero will
come to rest at a less accurate location. When the circular motion
of the microtome wheel approaches zero then the product of the
position and momentum approaches the Kerplank's constant. Thus using
more mathematical rigor the conjugate quantity of the sections
becomes the standard deviation from the property of being silver.
Associated with this pause in the angular momentum of the microtome
will be section chatter due to wave-particle duality. Ultramicrotome
manufactures have tried to address this problem with an adaptor known
as the von Neumann measurement, which according to some is better
than the Landau calculation.

I hope recalling this information lays to rest in no uncertain terms
the phenomenon of zero momentum block creep or in some cases inverse
block creep.

Bob Blystone



==============================Original Headers==============================
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6, 17 -- Subject: Differential Ultramicrotomy
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From: TindallR-at-missouri.edu
Date: Tue, 30 Jan 2007 09:36:46 -0600
Subject: [Microscopy] Differential Ultramicrotomy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Zowie, Bob! Can I have your autograph?

Randy

-----Original Message-----
X-from: rblyston-at-trinity.edu [mailto:rblyston-at-trinity.edu]
Sent: Tuesday, January 30, 2007 9:32 AM
To: Tindall, Randy D.

To all:

The chucked block in the unattended ultramicrotome obeys the Hehesenberg
Uncertainty Principle. As I trust you remember the position of a
chucked block whose momentary momentum is zero will come to rest at a
less accurate location. When the circular motion of the microtome wheel
approaches zero then the product of the position and momentum approaches
the Kerplank's constant. Thus using more mathematical rigor the
conjugate quantity of the sections
becomes the standard deviation from the property of being silver.
Associated with this pause in the angular momentum of the microtome will
be section chatter due to wave-particle duality. Ultramicrotome
manufactures have tried to address this problem with an adaptor known as
the von Neumann measurement, which according to some is better than the
Landau calculation.

I hope recalling this information lays to rest in no uncertain terms the
phenomenon of zero momentum block creep or in some cases inverse block
creep.

Bob Blystone



==============================Original
Headers==============================
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Ultramicrotomy 6, 17 -- Date: Tue, 30 Jan 2007 09:31:04 -0600 6, 17 --
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From: pbond-at-plymouth.ac.uk
Date: Tue, 30 Jan 2007 10:35:08 -0600
Subject: [Microscopy] RE: Differential Ultramicrotomy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

And I thought it was loads more complicated than that!

Pet




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Tel: 44 (0)1752 233092
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From: glenmac-at-u.washington.edu
Date: Tue, 30 Jan 2007 11:47:31 -0600
Subject: [Microscopy] Re: basic question ultramicrotopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

During the cutting process, the blade is compressing the remainder of
the block, which then expands afterwards.
My mentors led me to believe the overlap with the knife was due to
expansion of the block whenever it was allowed to 'rest'. The
forces equalize when sectioning steadily. This is one reason
microtomes for ulta-thin or semi-thin ( {3 µm) sections retract
during the return stroke.
I don't know if this is really the case, but it does seem to account
for the fact that we can break knives and blocks if not separated
before resuming sectioning.

Regards,
Glen

Glen MacDonald
Core for Communication Research
Virginia Merrill Bloedel Hearing Research Center
Box 357923
University of Washington
Seattle, WA 98195-7923 USA
(206) 616-4156
glenmac-at-u.washington.edu

************************************************************************
******
The box said "Requires WindowsXP or better", so I bought a Macintosh.
************************************************************************
******


On Jan 30, 2007, at 2:09 AM, nizets2-at-yahoo.com wrote:

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} Hello colleagues,
}
} I have (again) a pretty basic, non-life-threatening
} question about ultramicrotomy (with a Leica EM UC6 in
} my case).
} When I am cutting ultrathin sections it may happen I
} have to pause for a while (because I have to sign
} autographs, or to pick up sections from water). After
} the pause when I start the cutting cycle again, it
} very often does not cut immediately but I have to cut
} 500nm or more to touch the block again.
} I wondered if this was a sort of automatic protection
} from the machine, which retracts a little after some
} time of inactivity to avoid damage to the knife, or if
} this a the normal consequence of an effect of physics.
} Any comment on this?
}
} Stephane
}
}
}
}
} ______________________________________________________________________
} ______________
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} Get new email alerts with the free Yahoo! Toolbar.
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From: opmills-at-mtu.edu
Date: Tue, 30 Jan 2007 12:05:15 -0600
Subject: [Microscopy] poor mans TEM camera

Contents Retrieved from Microscopy Listserver Archives
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All,

Our digital TEM camera is dead, the TEM is on it's last leg so I am
not going to have the camera repaired. We use this TEM for teaching
and it is difficult to teach without a camera. I am thinking that
there may be a way to install a removable low cost video camera on
the binocular eyepiece for some applications - like teaching. Has
anyone done this?

Owen

Owen P. Mills
Director, Materials Characterization & Fabrication Facilities
Electron Optics Engineer, Applied Chemical & Morphological Analysis
Laboratory

Materials Science & Engineering
Michigan Technological University
Rm 512 M&M Bldg.
Houghton, MI 49931
PH 906-369-1875
FAX 906-487-2934
mailto:opmills-at-mtu.edu
http://www.mm.mtu.edu/~opmills



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From: garsha-at-itg.uiuc.edu
Date: Tue, 30 Jan 2007 14:55:32 -0600
Subject: [Microscopy] tolerance specifications for standard microscope slide?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Does anybody happen to know of a reference or resource for the size and
tolerance specifications for a conventional 25x75 mm microscope slide?
Thanks in advance.

--
Karl Garsha
Head Applications Scientist
Roper Bioscience Advanced Microimaging Group
3440 E. Brittania Drive
Tucson, AZ 85706
Office: 520-547-2704


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From: tina-at-pbrc.hawaii.edu
Date: Tue, 30 Jan 2007 14:59:18 -0600
Subject: [Microscopy] basic question ultramicrotopy (fwd)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi, Stephane-

I've had it both ways; block retracts and when I restart it misses, or
block expands and when I restart it whacks the knife. Depends on the type
of resin, how hard it is, the sample in the resin, temperature, humidity,
all kinds of things. Some of my stuff compresses with lengthy sectioning
and the "relaxes" forward when I stop. Other times the block seems to heat
up and be expanding during sectioning, then cools off and retracts when I
stop. In all cases I have learned to retract the knife before starting
again, just in case. Annoying, yes, but better safe than sorry.

I had one customer who had lots of material embedded in LR White many,
many years ago, and brought them in whenever she wanted to try a new
antibody. I had become familiar with them and knew that over time the
tissue (squid parts) expanded out of the face of the blocks. I guess the
tissue was softer than the surrounding resin. I used this "feature" once
when all the TEMs in the state were down and they were desparate to see
if they had immunogold staining. I put the labeled grids in the SEM and
was able to see the features of the tissue by topography, and the
colloidal gold sitting on top.

Please; no autographs!

Aloha,
Tina

} I have (again) a pretty basic, non-life-threatening question about
} ultramicrotomy (with a Leica EM UC6 in my case).
} When I am cutting ultrathin sections it may happen I have to pause for a
} while (because I have to sign autographs, or to pick up sections from
} water). After the pause when I start the cutting cycle again, it very
} often does not cut immediately but I have to cut 500nm or more to touch
} the block again.
} I wondered if this was a sort of automatic protection from the machine,
} which retracts a little after some time of inactivity to avoid damage to
} the knife, or if this a the normal consequence of an effect of physics.
} Any comment on this?
}
} Stephane
}
}
}
}
} ________________________________________________________________________
} ____________
} It's here! Your new message!
} Get new email alerts with the free Yahoo! Toolbar.
} http://tools.search.yahoo.com/toolbar/features/mail/
}
} ==============================Original
} Headers==============================
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****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************




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From: beaurega-at-westol.com
Date: Tue, 30 Jan 2007 17:18:13 -0600
Subject: [Microscopy] Re: basic question ultramicrotomy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Stephane,

Your question has three answers. The block can move away from the knife
edge, towards it, or stay at the same distance. The main governing factors
are the mechanical advance setting, temperature, and other heating (or
cooling) affects.

Most microtomists would like to believe that a mechanical advance setting
on a microtome determines the final thickness of sections. The mechanical
advance is a guideline or course adjustment of how thick your sections are.
The final thickness is the sum of the positive mechanical advance and the
± thermal advance.
The section's interference color tells you how thick the section really is
as it floats on water. This assumes you are not cutting something like
polyurethanes or high index eyewear lenses which "mess up" or shift the
interference colors on a thickness chart.

Your gap is caused by a discontinuous use of the mechanical advance and
block cutting while thermal affects continue to operate and you sign your
notebook.
If the block, arm or stage are warm and cooling, the gap will be seen as
you described. If the block is cold and warming up, then the thin sections
will be too thick.
If all the temperatures of all the equipment, the air, and your body &
fingers are the same; then the mechanical advance setting will probably be
real.

It was not unusual for me to see the block retreat from the knife edge at
first, later stabilize, and finally advance towards the knife edge during a
thin sectioning session on one block. You have to remember that your body
is giving off heat, you are touching adjustments on the chuck or arc
segment holder, the air temp might be varying, you picked up the diamond
knife and mounted it, you put cooler water in the knife boat, the internal
electronics are heating the cutting arm, water from the boat cools the
knife edge and block face, or maybe you just took off a cryo unit and the
arm and stage are still warming up. These thermal conditions have affects
on thickness' (and your gap).

If you are going to stop cutting for a few minutes, you should always back
up the knife about 5-10 microns and place the arm in the recommended
position. The knife should never be parked in front of the block face.
Failure to do so can result in a block of resin being shattered and/or a
knife being ruined.

One time I was cutting a very long and 0.25 mm wide block of an automotive
coating in cross section. My lab was located on bedrock and the microtome
was on an isolation table. My sections were being cut and I was getting
bright gold sections. I started the next section and it was gold. A guy
walked up to me and stood next to the microtome table but off to the side.
He was three feet away. The middle of that section changed to another
interference color. Just his standing there made a difference in the
interference color on the rest of that cut. I usually told these people to
not move and finished the next two sections and stopped.

Once I had stopped cutting a block and backed up the knife. When I
returned, the block face did not face off uniformly. It would cut a wedge
shape from right to left. That meant the block was deforming under just
the pressure of the clamping and I was using a "hard" Epon 812 clone epoxy
formulation.

The number of variables in use during microtoming of blocks for TEM quality
thin sections is high and sometimes subtle.

AO Reichert sold or gave away at their convention booths, a nice booklet on
microtoming. I think it was called "Ultramicrotomy---Faults and Problems".
It was a blue color and old. Maybe someone on the list can tell you how
to get a copy. It's worth getting but it is not a textbook. It was more
like a reprint of an article.

Microtomy is a learned skill just like riding a bicycle, only harder. Some
people just can't do it very well. Others could and we said they had "the
touch".

Paul


At 04:08 AM 1/30/07 -0600, you wrote:
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From: cgarber-at-2spi.com
Date: Tue, 30 Jan 2007 21:18:43 -0600
Subject: [Microscopy] Specifications on microscope slides

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Try http://www.supercircuits.com/ and type PC164C in search box. Sensitivity
will be adequate. Resolution is NTSC with composite video out. Can be viewed
on TV security monitor and/or digitized with inexpensive USB digitizer from
a computer store. It will work on a binocular with eyepiece removed. You may
have to use an adapter for an eyepiece (as for microscope/telescope) if you
care about particular field of view.

I would use instead a suitable C-mount lens and a gooseneck support from a
desk lamp - camera weight including lens is only a couple of ounces.
Position camera next to binoculars, and point it at a (tilted forward) large
viewing screen.

Should fit within $350 budget (camera, lens, power supply, USB digitizer or
a TV security monitor). Will work with some limitations...

Vitaly Feingold
SIA
2773 Heath Lane
Duluth GA 30096
Ph. 770-232-7785
Fax 770-232-1791
www.sia-cam.com
----- Original Message -----
X-from: {opmills-at-mtu.edu}
To: {vitalylazar-at-att.net}
Sent: Tuesday, January 30, 2007 1:06 PM

Karl Garsha wrote:
==============================================
Does anybody happen to know of a reference or resource for the size and
tolerance specifications for a conventional 25x75 mm microscope slide?
Thanks in advance.
===============================================
What is "conventional" in one part of the world is not necessarily the same
as what would be "conventional" in some other part of the world. So-called
standard microscope slides are made both as 1" x 3" and in other instances,
25 x 75 mm. It usually does not make a difference but if in your case it
does, it is less a matter of talking about tolerances than it is in terms of
specifying the right size. Probably the 1" x 3" are more commonly
available.

There are other instances in the microscopy world where we have a similar
kind of "situation". For example, the small "JEOL" SEM mounts, in North
America are specified as 3/8" but in other countries, they are specified as
10 mm. In general, the mounts are interchangeable in the various SEMs but
the problem comes when one wants to put these mounts into storage boxes.
Those made for 3/8" won't accommodate a 10 mm mount, and vice versa.

Chuck

===================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
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From: beadrysdale-at-yahoo.com
Date: Wed, 31 Jan 2007 08:30:49 -0600
Subject: [Microscopy] viaWWW: Outlook for microscopy technicians

Contents Retrieved from Microscopy Listserver Archives
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Email: beadrysdale-at-yahoo.com
Name: Bea Drysdale

Organization: unaffiliated

Title-Subject: [Filtered] Job outlook for microscopy technicians

Question: Hello. I am considering undertaking a two-year associate degree program to become a microscopy technician. I was wondering if anyone would be willing to comment on the job prospects I might have as new graduate with no science experience beyond this associate degree. The college's placement stats. look good but I would like a second opinion. Also, I am in my early forties and wonder if that would work against me finding a job (one of the draws of microscopy is that it is such a specialized skill that I would think employers would look beyond age). Thanks for any help you can give me.

---------------------------------------------------------------------------

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From: lcgould-at-med.cornell.edu
Date: Wed, 31 Jan 2007 08:55:50 -0600
Subject: [Microscopy] Re: viaWWW: Outlook for microscopy technicians

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear Bea,
I can address your concern from my perspective as the director of a
Core microscopy facility at an Ivy League Med school. When I am in
the market for new staff, it is like a breath of fresh air to find
someone with training. In the past 6 years, I've hired 2 techs, both
of whom were fresh out of college (4-year). Each of them had
exposure to, but no experience in microscopy and needed training from
the ground up. I was fortunate that tech #2 started working while
tech #1 was still here, so that #1 could train #2, with less imput
from me. The first time around, I had to do all of the hands-on
training, and it took me away from much of my other work. Finding
someone with a microscopy background would be a godsend.
Good luck,
Lee Cohen-Gould
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy & Histology Core Facility
Manager, Optical Microscopy Core Facility
Weill-Cornell Medical College

voice (212)746-6146
fax (212)746-8175
http://www.cornellcelldevbiology.org
http://www.cornellbiochem.org

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From: TindallR-at-missouri.edu
Date: Wed, 31 Jan 2007 09:36:37 -0600
Subject: [Microscopy] viaWWW: Outlook for microscopy technicians

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Bea,

I agree with Leona that the job possibilities in microscopy are good for
people with experience and training, and I suspect that being in your
40's would not be a significant factor. It may even work in your favor,
since people (at least the ones who have never met me) might assume that
age equals maturity, stability, responsibility, etc.

That said, there is a factor that is seldom discussed, but definitely
exists. While virtually all job advertisements ask for experience and
training, it is possible to end up in a situation where procedures and
attitudes in a lab are so set in stone that much of the training you
bring to a job will have to be thrown out the window. This is sometimes
due to good reasons, i.e., standard operating procedures are in place
for tracking, verification, and certification reasons, or a lab does a
certain type of work and has really worked out the best ways to
efficiently get the job done that leave little room for variation.
Other times this can simply be a result of micromanagement and other
less-noble reasons. In cases like these, it may be that what the lab
REALLY wants is to train someone from the ground up, but they didn't
really know that. It can be a shocker when it happens.

My point is that I would encourage you to get your degree and go for it.
The microscopy world is full of wonderful and rewarding careers. But be
prepared to be flexible and open to suggestion when joining a lab. My
experience is that virtually everybody has their own, sometimes
radically different, ways of doing things and many people don't accept
change easily or quickly. Be prepared to blend in and as time goes on
you may find that your ideas and training will be allowed to express
themselves, or you may find that the people that hire you have better
ideas that you can learn from. Or, and this is where it gets good, you
will likely find that your new associates are interested in the new
ideas and attitudes you bring to the lab, as well as passing along their
own hard-won experience to you.

Best of luck,
Randy

Randy Tindall
Senior EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W125 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu
On-line calendar:
http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=
Week&NavType=Both&Type=TimePlan


-----Original Message-----
X-from: beadrysdale-at-yahoo.com [mailto:beadrysdale-at-yahoo.com]
Sent: Wednesday, January 31, 2007 8:33 AM
To: Tindall, Randy D.

This Question/Comment was submitted to the Microscopy Listserver using
the WWW based Form at
http://www.microscopy.org/MicroscopyListserver/MLFormMail.html
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---
Remember this posting is most likely not from a Subscriber, so when
replying
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Listserver
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---

Email: beadrysdale-at-yahoo.com
Name: Bea Drysdale

Organization: unaffiliated

Title-Subject: [Filtered] Job outlook for microscopy technicians

Question: Hello. I am considering undertaking a two-year associate
degree program to become a microscopy technician. I was wondering if
anyone would be willing to comment on the job prospects I might have as
new graduate with no science experience beyond this associate degree.
The college's placement stats. look good but I would like a second
opinion. Also, I am in my early forties and wonder if that would work
against me finding a job (one of the draws of microscopy is that it is
such a specialized skill that I would think employers would look beyond
age). Thanks for any help you can give me.

------------------------------------------------------------------------
---

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From: frank.karl-at-degussa.com
Date: Wed, 31 Jan 2007 09:58:20 -0600
Subject: [Microscopy] Re: viaWWW: Outlook for microscopy technicians

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Bea,
I'm always glad to see people go into microscopy, regardless of their age.
And yes I believe employer looks beyond age as it take years to acquire the
experience and background to be a general purpose microscopist. You must
never stop learning. From this point on, is where I differ from most of
the feed back I suspect you're going to get. It's just my experience and
my opinion. Please send death threats off line to prevent soaking up the
bandwidth.

In todays market I am seeing BSs hired to do the work that technicians did.
I see MSs hired to do the job that chemists with BSs did 20 years ago. It
appears that only PhDs count. This seem to be the trend in large
companies, especial those with government contracts or grants. Many
companies will assume that you can teach microscopy to anyone off the
street because you're just lookin' at stuff. SEM/EDS/TEM/STEM/microtome,
well they are computer run aren't they? Ever manager I know thinks they
can interpret photomicrographs, especially after I annotate them.

I have seem incredible bright technicians, masters of their trade, people
capable of good scientific, independent work locked into positions that
they have outgrown and are actively working at a significantly higher level
but frozen to title and pay grade (and privileges) because they don't have
a BS or better.

I am and remain a microscopist because I love the work and windows to the
world it opens for me. I have had the opportunity to meet and hob nob with
some of the best. It's the best job in the world and I really love my
current position, but I think you should realize that some opportunities
and options will remain closed to you with only a two year degree, just as
some remain closed with a BS or MS.

Stay safe............Frank






beadrysdale-at-yahoo
.com To: frank.karl-at-degussa.com
cc:
01/31/2007 09:32 Subject: [Microscopy] viaWWW: Outlook for microscopy technicians
AM
Please respond to
beadrysdale








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Email: beadrysdale-at-yahoo.com
Name: Bea Drysdale

Organization: unaffiliated

Title-Subject: [Filtered] Job outlook for microscopy technicians

Question: Hello. I am considering undertaking a two-year associate degree
program to become a microscopy technician. I was wondering if anyone
would be willing to comment on the job prospects I might have as new
graduate with no science experience beyond this associate degree. The
college's placement stats. look good but I would like a second opinion.
Also, I am in my early forties and wonder if that would work against me
finding a job (one of the draws of microscopy is that it is such a
specialized skill that I would think employers would look beyond age).
Thanks for any help you can give me.

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From: TrogadisJ-at-smh.toronto.on.ca
Date: Wed, 31 Jan 2007 10:23:30 -0600
Subject: [Microscopy] Re: viaWWW: Outlook for microscopy technicians

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi, Bea

This reply is dry and not passionate - don't let that fool you, I
really love microscopy and my job.

When you mention a lack of science background - are you talking about
biological or electronics type of science? If you have no biological
science experience, you could look for work in a clinical lab where
protocols are pretty well established and have to be standardized. If
the research field appeals to you, this may prove to be challenging and
you may want to increase your biology background as much as possible -
for example, to be able to interpret what you see in a microscope and
figure out whether an image is 'real' and significant or whether the
equipment needs a tune-up. It helps if you understand whatever you see.

These days of digital imaging and complex microscope systems, an
understanding of the electronics is also an asset - make sure your
course covers that adequately. Another area of microscopy is in the
material sciences for which biology is not essential. This also requires
some knowledge of different areas of science. Also, there are always
jobs working for one of the microscope manufacturers or retailers.

The age factor is not an issue these days, competence is more
important. There is always a need for this specialty and actually older
people have more experience of having come across a particular problem
or have done a specific technique - so keep your eyes open especially
for troubleshooting tricks.

Let me know if you need any information, encouragement, whatever.
Good luck.
Judy



Title-Subject: [Filtered] Job outlook for microscopy technicians

Question: Hello. I am considering undertaking a two-year associate
degree program to become a microscopy technician. I was wondering if
anyone would be willing to comment on the job prospects I might have
as
new graduate with no science experience beyond this associate degree.
The college's placement stats. look good but I would like a second
opinion. Also, I am in my early forties and wonder if that would work
against me finding a job (one of the draws of microscopy is that it is
such a specialized skill that I would think employers would look
beyond
age). Thanks for any help you can give me.



Judy Trogadis
Bio-Imaging Coordinator
St. Michael's Hospital, 7Queen
30 Bond St.
Toronto, ON M5B 1W8, Canada
ph: 416-864-6060 x6337
pager: 416-685-9219
fax: 416-864-6043
trogadisj-at-smh.toronto.on.ca


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From: nizets2-at-yahoo.com
Date: Wed, 31 Jan 2007 10:52:54 -0600
Subject: [Microscopy] Re: basic question ultramicrotopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Thanks to all for your answers.
I don't know why but the vast majority didn't take
seriously the possibility that I may stop cutting to
sign autographs. I will remember this ;-)

In a room with air conditioning, all doors closed and
where all conditions are there to offer an optimal
stable environment, I couldn't believe that
temperature changes could have such effects.
Apparently I was wrong, as most of you point this as
the main cause, even if other obscure reasons have
been proposed too (and even if I would tend to believe
the second category more than the first one).
It is strange to see how a so common technique which
delivers such high quality data can be subject to the
same common uncontrollable events.
Just to be fair I'll finish by pointing out that my
Leica ultramicrotome is near from perfection, it is a
real delight to use and cannot be taken responsible
for this problem.

Stephane



____________________________________________________________________________________
Yahoo! Music Unlimited
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From: gwe-at-ufl.edu
Date: Wed, 31 Jan 2007 11:30:39 -0600
Subject: [Microscopy] Re: viaWWW: Outlook for microscopy technicians

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

One thing I might add to the comments you have already gotten is that
you need to be prepared to relocate, Perhaps clear across the country.
My last hire was living in California and came to work here in Florida
for an entry level job.
Second, lack of experience may be to your advantage since many hires
are at entry level and folks with more experience are going to want more
money. You are also going to find that jobs in the engineering area
will pay higher than those in the biological area.

Greg Erdos
University of Florida, Retired.

beadrysdale-at-yahoo.com wrote:
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} Title-Subject: [Filtered] Job outlook for microscopy technicians
}
} Question: Hello. I am considering undertaking a two-year associate degree program to become a microscopy technician. I was wondering if anyone would be willing to comment on the job prospects I might have as new graduate with no science experience beyond this associate degree. The college's placement stats. look good but I would like a second opinion. Also, I am in my early forties and wonder if that would work against me finding a job (one of the draws of microscopy is that it is such a specialized skill that I would think employers would look beyond age). Thanks for any help you can give me.
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From: iancozine-at-gmail.com
Date: Wed, 31 Jan 2007 14:34:42 -0600
Subject: [Microscopy] viaWWW: Job outlook for microscopy technicians

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Email: iancozine-at-gmail.com
Name: Ian Cozine

Organization: MATC

Title-Subject: [Filtered] Job outlook for microscopy technicians

Question: Bea,

I am a 2nd year student at the EM program at MATC in Madison,
Wisconsin and I graduate this May. While I can't add many informed
comments about the outlook for technicians yet, I can say that I have
thoroughly enjoyed the program. It is a small program so there is
plenty of one on one instruction. We get to spend several hours a
week in the lab, and from day one we are working with the
instruments. I think this is one advantage over a B.S. degree. Upon
graduating I will have 2 years working with all sorts of equipment
and techniques and I think that most 4 year degrees aren't going to
provide that much hands on experience.

I do know that the placement rates are quite good. If you graduate
from the program and are reasonably proficient you will get a job.
Our instructors do a really great job of locating job leads for us
all over the country. One consideration, however, is that chances are
you will be moving for your first job.

I would be hard-pressed to think of another 2 year program where upon
graduation you will get to do such interesting work. I would not
hesitate recommending a microscopy degree to anyone.


-Ian

Email: beadrysdale-at-yahoo.com
Name: Bea Drysdale

Organization: unaffiliated

Title-Subject: [Filtered] Job outlook for microscopy technicians

Question: Hello. I am considering undertaking a two-year associate
degree program to become a microscopy technician. I was wondering
if anyone would be willing to comment on the job prospects I might
have as new graduate with no science experience beyond this associate
degree. The college's placement stats. look good but I would like a
second opinion. Also, I am in my early forties and wonder if that
would work against me finding a job (one of the draws of microscopy
is that it is such a specialized skill that I would think employers
would look beyond age). Thanks for any help you can give me.

---------------------------------------------------------------------------

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From: johnwillis-at-valcor.com
Date: Wed, 31 Jan 2007 14:45:47 -0600
Subject: [Microscopy] Camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hello:
I use a 3.2 Megapixel Digital camera PN OMCAM32 from The Microscope Store
for OPTICAL microscopy with great results. The camera mounts thru the
eyepiece and has an LCD so you can see exactly what you are getting. The
brighter the light the better the image but I get images in dimmer light
that I can use. The pictures are stored on an internal memory card which is
then downloaded to a PC via cable. It requires no special software for
download.

I talked to Eric Naff. The OMCAM32 is not listed on the web site, but the
store will reply "yes" to an e-mail request regarding it.

The Microscope Store
316 Windy Pines Lane
Rocky Mount, VA 24151
Voice (877) 409-3556
FAX (540) 489-4785
2rhonda-at-microscope-store.com
www.microscope-store.com

I hope this helps,

John R Willis
Tech Writer
Valcor Eng Corp
2 Lawrence Rd
Springfield, NJ 07081
Voice (973) 467-8400 X 7257
FAX (973) 467-8382
johnwillis-at-valcor.com
www.valcor.com


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From: john.brealey-at-imvs.sa.gov.au
Date: Wed, 31 Jan 2007 19:12:51 -0600
Subject: [Microscopy] Ultratsructure of Dendritic Cells

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Listers,

Are there any ultrastructural differences between monocytes and
monocyte-derived dendritic cells?
I've been looking at marmoset monocytes, immature dendritic cells and
matrure dendritic cells and am having difficulties differentiating between
them at the ultrastructural level.

Are there any references available to view images of these cells?

Regards,

John Brealey
EM Unit
Queen Elizabeth Hospital
Adelaide
South Australia


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From: bfoster-at-mme1.com
Date: Thu, 1 Feb 2007 07:47:35 -0600
Subject: [Microscopy] Re: EM nanoemulsion

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear Dotty,

There is an intriguing new technology which is under development that
might solve this problem. NT-MDT has integrated an AFM with an
ultramicrotome (Leica UC6-NT), called NTegra Tomo. The developer,
Dr. Anton Efimov, has recently been experimenting with a cryo
version. Tomo has proven very helpful in imaging and elucidating 3D
nanostructures for things like dried emulsions and biological
entities (c. elegans). If the nanoemulsion is cryo-stable, the cryo
version of Tomo should be a good solution. Also, because the AFM
uses local differences in elasticity to image different phases, there
would be no need to stain.

Please contact me off-line if you would like to have Dr. Efimov try
your investigator's sample as part of his test program.

Hope this was helpful,

Best regards,
Barbara Foster

Microscopy/Microscopy Education
313 S Jupiter Rd, Suite 100
Allen, TX 75002
P: 972-954-8011
W: www.MicroscopyEducation.com


MME is now scheduling customized, on-site courses through July. Call
us today for details.

P. S.
Need a good general reference or light microscopy text for the Spring
semester? Call us today to learn more about "Optimizing LIght
Microscopy". Copies still available through MME... even for
class-room lots ... and we give quantity discounts. Call Ken Piel at
(972)954-8011.



At 10:10 AM 10/27/2006, dsoren-at-umich.edu wrote:



} ----------------------------------------------------------------------------
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From: TindallR-at-missouri.edu
Date: Thu, 1 Feb 2007 08:42:34 -0600
Subject: [Microscopy] Maleate vs. maleic vs. malic, YO

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Thanks to everyone who responded to my maleic acid buffer query. We now
have some things to try.

By the way, the following response is irresistable, courtesy of the
incomparable Snoop Leunissen (Jan, that is). Foshizzle, microscopists
rock, dog.

Enjoy.

Randy

"Buffer Rap

For anyone who likes to do EM
buffers at times are a hell of a wham
the chemistry lacks in every way
yet we like to have a good display

So what is this all about mall ee 8?
Does that stuff accommodate uranyl acetate?
at what pH, what ionic strength?
I better ask the LIST for some reference

With some advice here and a helping hand I am sure I can pretend I
understand So I take some stuff from the lab supply Mix it together and
hope I will get by

Wow, man, what happens, it's workin' alright!
The negative stain is clear and bright!
No precipitate, the structures they are fine It works! Now I can advice
the next in line.

Anyway, for mallE8 to work alright, you see you need two solutions, mark
them A and B Empty bottle (C) in the middle now that should do And
mixing left and right will be the clue

Solution A has sodium hydrogen maleate
23.2 grams if it's trihydrate
Dissolve in 200 ml 1M Sodium Hydroxide
And make to 1 liter with distilled water alright

Solution B is simple
just point 1 Molar NaO-age
Solution C is the trick,
Now don't get into a rage!

Chorus:
Take 25 mls out of bottle A
transfer to Bottle C without further delay Mix in x mls of B, top up to
100 cc Get approximate pH from the listing you will see

pH x ml 0.1 M NaOH

5.2 7.2
5.4 10.5
5.6 15.3
5.8 20.8
6.0 26.9

For Tris maleate it is much the same
Two stocks again, it's almost lame
The first holds Tris as well as Maleic acid
24.2 and 23.2 grams, yep that's it!

And before I forget,
make a liter of that
now the other stock again is just NaO-age a plain 0.2 Molar is all that
it takes

Chorus...

pH x ml 0.2 M NaOH

5.4 5.4
5.6 7.75
5.8 10.25
6.0 13.0

--------------

Apologies, the listings don't rhyme.
Disclaimer: I will not be responsible for anyone getting hurt while
trying to rap the numbers!

X-from: "Data for Biochemical research", by Dawson, Elliot, Elliot and
Jones Clarendon Press, Oxford, 3rd ed.

Recipes for maleate buffer (J.Am.Chem.Soc 51 (1929), 1754) and
Tris/maleate buffer (PSEBM 68 (1948) p354 or Meth Enzym. 1 (1955)
138"



Randy Tindall
Senior EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W125 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu
On-line calendar:
http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=
Week&NavType=Both&Type=TimePlan


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From: microtomy-at-gmail.com
Date: Thu, 1 Feb 2007 13:40:02 -0600
Subject: [Microscopy] Re: LASIK and microscopists

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Since the list played a big part in my decision to go through with
LASIK 2.5 years ago, I'll put in 2 cents on this topic as well.

My doctor did warn about floaters, halos, possible mis-correction as
side effects and then gave me a realistic assessment of what it would
mean should I suffer these side effects. He spent a great deal of
time addressing my concerns and assured me that such problems were
quite rare and often very minor.

In the 2 months following the surgery, I experienced halos and
starburst patterns around streetlights and headlights while driving at
night. Eventually these symptoms subsided and I see less
starburst-type patterns now than I ever did before the surgery. At
2.5 years post surgery, I now have a slight floater in my right eye
which I rarely notice unless conditions are just so. I never noticed
it at all until 4-5 months ago. That said, I know several friends and
family members who have floaters that have NEVER had LASIK surgery, so
I do not feel confident that LASIK was the cause.

All in all, the improvement in my vision from about 20:250 to 20:20
has been an immensely positive development. I would recommend LASIK
to anyone who has been declared a good candidate by reputable eye
surgeon. Do your research on the physician. I had no less than 4
recommendations from optometrists with no connection to each other. I
also spoke with 3 patients he had treated in the past, so I felt
pretty comfortable that he was competent.

For anyone, especially a microscopist, your vision isn't something
to bargain shop for. One place in town offers LASIK "as low as $599
per eye". It didn't take long to find out that they have higher
complication rates. Ultimately, I paid close to $3,000 for both eyes
and was confident that I was getting the best treatment available in
the area.

Cheers,
Jay

On 1/29/07, Jessica.Wagner-at-childrens.harvard.edu
{Jessica.Wagner-at-childrens.harvard.edu} wrote:
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
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} ----------------------------------------------------------------------------
}
} John,
}
} I missed that thread, but I'll be happy to add my two cents now.
} Assuming the PVD (posterior vitreous detachment) doesn't get any worse,
} for me the benefits of LASIK still outweigh this minor negative. The
} 'floater' that I see is an annoyance, but it doesn't truly inhibit my
} vision through the scope (or outside the scope for that matter).
}
} But I had LASIK only a year ago, and was never warned PVD could be a
} complication, so this says to me that there is still much unknown about
} the procedure and it's results. Unfortunately, complications of laser
} eye surgeries really aren't tracked all that well; doctors are not
} required to report them, unless they are related to a device, but even
} then, many doctors are not aware of FDA regulations about device event
} reporting or how/to whom they should report adverse events.
}
} Here is an article about PVD's and LASIK:
} http://www.springerlink.com/content/j3100858467pu5k1/
}
} And thanks to everyone who offered imaging advice!
}
} Jessica
}
}
} -----Original Message-----
} X-from: john.mardinly-at-intel.com [mailto:john.mardinly-at-intel.com]
} Sent: Monday, January 29, 2007 12:56 PM
} To: Wagner, Jessica
} Subject: [Microscopy] RE: imaging the occular view through another port?
}
}
}
}
}
} ------------------------------------------------------------------------
} ----
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} America To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
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} ------------------------------------------------------------------------
} ----
}
} Jessica;
} Some time ago, there was a thread about LASIK and microscopists.
} I do not recall any of the reports mentioning this sort of post-surgical
} vision difficulty. Is this perhaps a subject that should be re-visited?
}
} John Mardinly
} Intel
}
} Disclaimer: The opinions of this author do not represent the opinions of
} Intel Corporation.
}
} -----Original Message-----
} X-from: Jessica.Wagner-at-childrens.harvard.edu
} [mailto:Jessica.Wagner-at-childrens.harvard.edu]
} Sent: Friday, January 26, 2007 12:25 PM
} To: Mardinly, John
} Subject: [Microscopy] imaging the occular view through another port?
}
}
}
}
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}
} Hello list. I have a question that's just for fun. I'm using a Nikon
} TE-2000 wth all 4 ports and a beam splitter that can direct light 50/50
} split between two ports. Is there a way to set up a camera to image not
} a sample but specifically the image that I'm seeing? I have a minor
} defect in my eye, a wrinkle caused by slight detachment of the vitreous
} (I think due to having LASIK done). When I look through the scope at a
} bright field, I can see an image of the wrinkle. I'm curious to know if
} I can capture it, but my guess is that the image only exists in the
} occulars? Maybe I could somehow use a dichroic mirror?
}
} Thanks,
} Jessica
}
}
}
} ==============================Original
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From: jehrman-at-mta.ca
Date: Thu, 1 Feb 2007 14:22:41 -0600
Subject: [Microscopy] LASIK and microscopists

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

This isn't about LASIK, but I caught the bit about floaters and just
want to relay my experience, if only to
prevent others from going through the ordeal I did last summer.

Don't ignore floaters, even if they've been noticeable for a long time.
My right eye always had a significant
number of them, and my optometrist told me to come back if I noticed an
increased number of them. That's
not very easy quantify over time, but in hindsight the number probably
did increase in the months before I had a partial
detachment of the retina. No other symptoms until a ominous black spot
appeared in the corner of my vision.
The surgeon said that the whole thing could have fallen off at any time,
probably resulting in total blindness
in that eye. Fortunately surgery corrected everything, and six months
later I have nearly perfect (well, as perfect
as it was before) vision again in that eye.

Get your eyes dilated and checked for retinal tears *every* time you
have an eye exam, especially if you're over
40. I know, it's a pain, but losing binocular vision is a much bigger
pain! Retinal detachment is *not* most common
in boxers, drag racers, sky divers - people who get their heads banged
around a lot. It happens most often to people
who are strongly nearsighted. I know quite a few of those in the
microscopy world and I don't think any of them would
be keen on saving operating expenses by only buying monocular scopes.

Cheers,

Jim

--

James M. Ehrman
Digital Microscopy Facility
Mount Allison University
Sackville, NB E4L 1G7
CANADA

phone: 506-364-2519
fax: 506-364-2505
email: jehrman-at-mta.ca
www: http://www.mta.ca/dmf


==============================Original Headers==============================
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From: Jane.LaGoy-at-bodycote.com
Date: Thu, 1 Feb 2007 15:38:25 -0600
Subject: [Microscopy] eye floaters

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

I can also add that the number of eye 'floaters' I have is increasing with
age (I'm 43), and I have never had eye surgery. The thought of trying to
zap them, and nothing else important, with a laser is terrifying, but it is
getting more difficult to use my light microscope. I am also very
nearsighted and wear contact lenses; does anyone know if there's a
correlation?

Thanks, Jane

Jane L. LaGoy
Laboratory Services Manager/
Development Engineer
Bodycote North America
155 River Street
Andover, MA 01810
978-470-1620 x450
FAX: 978-475-2951
jane.lagoy-at-bodycote.com
The only people to get even with are those who have helped you.





==============================Original Headers==============================
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From: r-holdford-at-ti.com
Date: Thu, 1 Feb 2007 17:53:30 -0600
Subject: [Microscopy] Re: eye floaters

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

I'm also extremely nearsighted and my floaters have increased with age.
Sometimes when I'm reading, I have to move my move head to get a pesky
one out of the way. I had them before wearing hard contacts for 12
years and I still have them. So I don't think there's a correlation but
I realize my opinion is not a scientific study. My optometrist once
explained to me why very near-sighted people should have their retinas
examined frequently for tears/detachments. Near-sighted eyeballs are
longer front to back than normal eyeballs. He said one can be born with
normal-sized retinas stretched to fit the larger eyeballs. (My father
is near-sighted; my mother is not.) These 'stretched' retinas are more
prone to tears/damage/detachments than normal ones. I have no training
in eye physiology so I was taking him at his word. As microscopists,
our eyes are more valuable than our hands, which are pretty darn
valuable. Take care of them.

Jane.LaGoy-at-bodycote.com wrote:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
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}
} I can also add that the number of eye 'floaters' I have is increasing with
} age (I'm 43), and I have never had eye surgery. The thought of trying to
} zap them, and nothing else important, with a laser is terrifying, but it is
} getting more difficult to use my light microscope. I am also very
} nearsighted and wear contact lenses; does anyone know if there's a
} correlation?
}
} Thanks, Jane
}
} Jane L. LaGoy
} Laboratory Services Manager/
} Development Engineer
} Bodycote North America
} 155 River Street
} Andover, MA 01810
} 978-470-1620 x450
} FAX: 978-475-2951
} jane.lagoy-at-bodycote.com
} The only people to get even with are those who have helped you.
}
}
}
}
}
} ==============================Original Headers==============================
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} 7, 29 -- To: "Microscopy Listserver (E-mail)" {Microscopy-at-MSA.Microscopy.com}
} 7, 29 -- Subject: eye floaters
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}

--
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Becky Holdford (r-holdford-at-ti.com)
972-995-2360
972-648-8743 (pager)
SC Packaging FA Development
Texas Instruments, Inc.
Dallas, TX
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~


==============================Original Headers==============================
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From: U.J.Potter-at-bath.ac.uk
Date: Fri, 2 Feb 2007 10:22:01 -0600
Subject: [Microscopy] SEM of Zebra fish

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listers,

I am about to prepare some zebra fish heads for SEM - to look at morphology
&
take some measurements. What do you think are the pros & cons of critical
point drying versus HMDS drying after fixation in GDA+PVP in SCB followed by
osmium+potassium ferrocyanide followed by staining in aqueous UA before
dehydration for small fish heads? I have had success with the HMDS drying
using cultured cells and insect tissue. The thermocirculator attached to my
CPD has problems - it is difficult to heat slowly enough so until I can
replace it I thought HMDS might be an alternative method.


Many thanks
Ursula
---------------

Ursula J. Potter
Centre for Electron Optical Studies (CEOS)
Building 3 West 2.15
The University of Bath
Claverton Down
Bath BA2 7AY
UK
Tel: 01225 385651
Email: U.J.Potter-at-bath.ac.uk

==============================Original Headers==============================
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5, 24 -- From: Ursula Potter {U.J.Potter-at-bath.ac.uk}
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From: rosslm-at-missouri.edu
Date: Fri, 2 Feb 2007 10:44:05 -0600
Subject: [Microscopy] AMRAY 1600 on EBAY

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Hi,

If anyone is interested in an AMRAY 1600 the University of Missouri-Columbia
has just listed it on Ebay. Go to : www.surplus.missouri.edu then the eBay
items link.

Any questions feel free to contact me.
Lou Ross
Senior Electron Microscope Specialist
Electron Microscopy Core Facility
W136 Veterinary Medicine
University of Missouri
Columbia, MO 65211-5120
(573) 882-4777, fax 884=2227
email: rosslm-at-missouri.edu
http://www.emc.missouri.edu


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From: Michal.Jarnik-at-fccc.edu
Date: Fri, 2 Feb 2007 13:55:35 -0600
Subject: [Microscopy] SEM of cells in monolayer

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Dear Listers,

A colleague needs to SEM image cells growing in monolayer on a coverslip
in a thin layer of collagen matrix. I tried to prepare it more or less
usual way - aldehyde fixation in cacodylate (2% formaldehyde/2%
glutaraldehyde for about 90 min) followed by osmium, dehydration in
increasing concentration of ethanol, replacing ethanol with amyl acetate
and eventually CPD from carbon dioxide. The results are suboptimal at
best - cells seem to shring and get extracted. Does anybody have a good
idea they would like to share?

Thanks,

Michal

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From: Jane.LaGoy-at-bodycote.com
Date: Fri, 2 Feb 2007 14:02:25 -0600
Subject: [Microscopy] eye floater info - thanks!

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Thanks to all who responded to my inquiry; I see an opthamologist annually
but have not asked about this problem before, so I certainly will now.

Jane L. LaGoy
Laboratory Services Manager/
Development Engineer
Bodycote North America
155 River Street
Andover, MA 01810
978-470-1620 x450
FAX: 978-475-2951
jane.lagoy-at-bodycote.com
The only people to get even with are those who have helped you.





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From: oshel1pe-at-cmich.edu
Date: Fri, 2 Feb 2007 14:45:10 -0600
Subject: [Microscopy] Re: SEM of cells in monolayer

Contents Retrieved from Microscopy Listserver Archives
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Michal,

First, skip the amyl acetate, it's not needed.
Second, how many soak-purge cycles did you do in the CPD? I.e., Fill
the chamber, flush with lqCO2 until the EtOH is gone, let cells soak
X minutes, then purge with lqCO2, and repeat N times.
I found a monolayer on coversilps usually only needed 3 soaks for 5
minutes each, but sometimes could require 4 or 5 soaks.
If all of the EtOH (or amyl acetate) is not removed, you will get
serious shrinkage.

I also didn't bother with formaldehye or osmium, just 1.25% glut (2%
is pretty strong). Depending on the kV you're using, 1% OsO4 may be
helpful, though.
Fixation in OsO4 should only need an hour. Glut can go 1 to 2 hours.

What % EtOH did you start the dehydration at? I've used 30% and 50%
successfully, any higher is too high, and sometimes one needs to
start at 15%. How long in each EtOH step? 5 minutes should be enough.

And then, you will get some shrinkage no matter what you do.

Other things to try:
Hexamethyldisilizane (HMDS). After EtOH dehydration, go through a
2:1, 1:2 EtOH:HMDS, 3 X 100% HMDS series and then air-dry for 1 to 2
hours. Room temp. or at 60 deg. C, sometimes one works better than
the other. Do in a hood!

If you have the proper equipment, freeze-drying can work very well,
and since there are no chemical fixatives or dehydrating agents
involved the cells may be "more real".

Phil

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From: dac-at-research.umass.edu
Date: Fri, 2 Feb 2007 15:07:19 -0600
Subject: [Microscopy] Re: SEM of cells in monolayer

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Michal,

The protocol sounds very "usual" as you say, and I wonder if something
just went wrong along the way?

You didn't mention any osmotic agents in the fixative, sometimes
beneficial for cultured cells; various things and concentrations are
used. I just did some samples that used 7.5% sucrose in the fixatives
and buffer washes until the OsO4 was rinsed out.

A somewhat longer time in the glutaraldehyde for SEM is sometimes
beneficial; the cells toughen a bit - tip from our old Polaron CPD
manual...

Also, while I don't think it is the problem, it is not necessary to use
amyl acetate: using 100% ethanol works just fine for the CPD process.
Make sure the ethanol is really dry - store ethanol for final changes
over good molecular sieves - Type 3A, recently baked, ~5% of ethanol
volume, let is stand some days well sealed, don't stir up fines. The CO2
also needs to be a dry grade with good molecular sieve trap on the line.

Make sure to exchane well to get all the ethanol out. The cells on
coverglass should exchange quickly, but were they in some "capsule" that
limited exchange? Was the vapor phase clear when it went supercritical,
or hazy? Was there any smell of amyl acetate when you opened the chanmber?

Could the coverglasses have gone dry at some point - even in the CPD
process? Did you do this job yourself, or have an underpaid,
un-benefitted work-study student do the work? Sorry, that's not fair; I
used to be one; they aren't ALL inattentive.... The liquid drops as gas
phase pressure increases; maybe they got above the liquid surface? The
instructions printed on our Balzers CPD-030 unit say to "drain and
refill several times" but the sample should never really be drained from
liquid during the flushing exchanges; always do partial changes to keep
the sample submerged.

Hope this helps.

Dale Callaham
Central Microscopy Facility
c/o Microbiology Department
Morrill 4 N, Rm.1
University of Massachusetts
Amherst, MA 01003

http://www.bio.umass.edu/microscopy


Michal.Jarnik-at-fccc.edu wrote:
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} Dear Listers,
}
} A colleague needs to SEM image cells growing in monolayer on a coverslip
} in a thin layer of collagen matrix. I tried to prepare it more or less
} usual way - aldehyde fixation in cacodylate (2% formaldehyde/2%
} glutaraldehyde for about 90 min) followed by osmium, dehydration in
} increasing concentration of ethanol, replacing ethanol with amyl acetate
} and eventually CPD from carbon dioxide. The results are suboptimal at
} best - cells seem to shring and get extracted. Does anybody have a good
} idea they would like to share?
}
} Thanks,
}
} Michal
}
} ==============================Original Headers==============================
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From: stephenson-at-impactanalytical.com
Date: Fri, 2 Feb 2007 16:11:29 -0600
Subject: [Microscopy] viaWWW: Outlook for microscopy technicians

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Greetings Bea,
As an aging graduate of the associates degree program of the Madison Area
Technical College (MATC), I have read this thread with particular interest.
All the comments to date have been consistent with my experience. I entered
the program a couple of years prior to your age; another classmate was a
couple years older than you are now. We both have done well. I haven't
found age to be an impediment. I think your life experience, coupled with
the recent training that some employers find attractive, will make you a
good prospect if you do well in the program (as we all know you will!).

There are many opportunities that will be open to you with just the
associates degree, simply because these programs are well known in the small
world of the microscopy community for providing people who can walk into a
lab with the demonstrated unique little fiddly, intensely controlled skills
that we require to do our work. It is hard to know if someone coming in off
the street can do that stuff, even if they have more relevant academic
training. It is also true that there are opportunities that will be closed
to you, as another commenter observed.

The best action you can take to minimize the effect of your non-science
background is to be prepared to take on additional training to customize
your fit with whatever job you find. Microscopy contains many areas of
specialization, so it is likely that any new job will require some extra
training, for you or anyone. If you do well in the program, and project
your willingness to jump right back in with additional classes as a new-hire
(often this can be done on the employer's nickel), this combined with your
life experience will be attractive to many employers.

Having said that, I had to do the same thing and after 2 years of school and
a baby in the family it was a painful prospect! But it did make all the
difference in the new job. After five and a half years in the field I feel
very much a part of the microscopy community, and love the work. I hope you
decide to join us, Bea!
Sincerely,
Matt

-----Original Message-----
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Sent: Wednesday, January 31, 2007 9:38 AM
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Email: beadrysdale-at-yahoo.com
Name: Bea Drysdale

Organization: unaffiliated

Title-Subject: [Filtered] Job outlook for microscopy technicians

Question: Hello. I am considering undertaking a two-year associate degree
program to become a microscopy technician. I was wondering if anyone would
be willing to comment on the job prospects I might have as new graduate with
no science experience beyond this associate degree. The college's placement
stats. look good but I would like a second opinion. Also, I am in my early
forties and wonder if that would work against me finding a job (one of the
draws of microscopy is that it is such a specialized skill that I would
think employers would look beyond age). Thanks for any help you can give
me.

---------------------------------------------------------------------------

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From: dsherman-at-purdue.edu
Date: Fri, 2 Feb 2007 16:28:37 -0600
Subject: [Microscopy] Re: SEM of cells in monolayer

Contents Retrieved from Microscopy Listserver Archives
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We often run into the same problem. I think some occurs when cells are left
with minimal fluid for even very short times while dehydrating. Surface
tension is a real problem with very little fluid coverage when changes are
being made. It is best to leave a little fluid in the culture dish and do a
few more changes rather than risk the problems of excess shrinkage. Even
under the best of conditions, some shrinkage is inevitable.

Years ago I did a large number of cell cultures using ducupan resin to
infiltrate the cells. Cells were fixed and then infiltrated with successive
mixtures of Ducupan:H2O. The excess 100% resin was washed away using
propylene oxide and then cultures polymerized. This minimized shrinkage but
also you should expect that surface detail could also be obscured if any
resin remained on the outside of the cells.

This method did minimize breakage of long processes from axonal and
dendritic cells.

Debby

Debby Sherman, Director Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www.agriculture.purdue.edu/microscopy




} From: {Michal.Jarnik-at-fccc.edu}
} Reply-To: {Michal.Jarnik-at-fccc.edu}
} Date: Fri, 2 Feb 2007 13:59:27 -0600
} To: {dsherman-at-purdue.edu}
} Subject: [Microscopy] SEM of cells in monolayer
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Dear Listers,
}
} A colleague needs to SEM image cells growing in monolayer on a coverslip
} in a thin layer of collagen matrix. I tried to prepare it more or less
} usual way - aldehyde fixation in cacodylate (2% formaldehyde/2%
} glutaraldehyde for about 90 min) followed by osmium, dehydration in
} increasing concentration of ethanol, replacing ethanol with amyl acetate
} and eventually CPD from carbon dioxide. The results are suboptimal at
} best - cells seem to shring and get extracted. Does anybody have a good
} idea they would like to share?
}
} Thanks,
}
} Michal
}
} ==============================Original Headers==============================
} 4, 17 -- From Michal.Jarnik-at-fccc.edu Fri Feb 2 13:55:35 2007
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9, 23 -- From dsherman-at-purdue.edu Fri Feb 2 16:28:36 2007
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9, 23 -- Subject: Re: [Microscopy] SEM of cells in monolayer
9, 23 -- From: Debby Sherman {dsherman-at-purdue.edu}
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From: Ann.Lehman-at-trincoll.edu
Date: Fri, 2 Feb 2007 17:47:55 -0600
Subject: [Microscopy] Re: SEM of cells in monolayer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

When I was working with cultured fibroblast monolayers for SEM back in
the 70's, I used aldehydes and osmium, as most do, and experienced
ripped cells and ones that appeared to 'crack' along the surface,
particularly where long filopodia extended from the cell bodies. But, my
TEM looked fine. So for kicks, I tried my usual TEM protocol for the SEM
samples, which used the same aldehydes and osmium but also used uranyl
acetate as a post-fix. I had excellent results. No cracks or tears at
all.

Something you might wish to try.

Ann Hein Lehman
Assistant Director, Electron Microscopy Facility
Trinity College
300 Summit Street
Hartford, CT 06106
v. 860-297-4289
f. 860-297-2538
e. ann.lehman-at-trincoll.edu
w. http://www.trincoll.edu/~alehman


-----Original Message-----
X-from: dsherman-at-purdue.edu [mailto:dsherman-at-purdue.edu]
Sent: Friday, February 02, 2007 5:32 PM
To: Lehman, Ann R

We often run into the same problem. I think some occurs when cells are
left
with minimal fluid for even very short times while dehydrating. Surface
tension is a real problem with very little fluid coverage when changes
are
being made. It is best to leave a little fluid in the culture dish and
do a
few more changes rather than risk the problems of excess shrinkage.
Even
under the best of conditions, some shrinkage is inevitable.

Years ago I did a large number of cell cultures using ducupan resin to
infiltrate the cells. Cells were fixed and then infiltrated with
successive
mixtures of Ducupan:H2O. The excess 100% resin was washed away using
propylene oxide and then cultures polymerized. This minimized shrinkage
but
also you should expect that surface detail could also be obscured if any
resin remained on the outside of the cells.

This method did minimize breakage of long processes from axonal and
dendritic cells.

Debby

Debby Sherman, Director Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www.agriculture.purdue.edu/microscopy




} From: {Michal.Jarnik-at-fccc.edu}
} Reply-To: {Michal.Jarnik-at-fccc.edu}
} Date: Fri, 2 Feb 2007 13:59:27 -0600
} To: {dsherman-at-purdue.edu}
} Subject: [Microscopy] SEM of cells in monolayer
}
}
}
}
}
------------------------------------------------------------------------
----
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
America
} To Subscribe/Unsubscribe --
http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
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----
}
} Dear Listers,
}
} A colleague needs to SEM image cells growing in monolayer on a
coverslip
} in a thin layer of collagen matrix. I tried to prepare it more or less
} usual way - aldehyde fixation in cacodylate (2% formaldehyde/2%
} glutaraldehyde for about 90 min) followed by osmium, dehydration in
} increasing concentration of ethanol, replacing ethanol with amyl
acetate
} and eventually CPD from carbon dioxide. The results are suboptimal at
} best - cells seem to shring and get extracted. Does anybody have a
good
} idea they would like to share?
}
} Thanks,
}
} Michal
}
} ==============================Original
Headers==============================
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9, 23 -- Subject: Re: [Microscopy] SEM of cells in monolayer
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19, 25 -- From Ann.Lehman-at-trincoll.edu Fri Feb 2 17:47:55 2007
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From: murphyjudy-at-comcast.net
Date: Fri, 2 Feb 2007 22:16:22 -0600
Subject: [Microscopy] Re: SEM of cells in monolayer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Michal,
When I did cultured cells on coverslips found problems like you were
experiencing.

1. We changed to Parducz fixative instead of Osmium which is a
wonderful hardener for filipodia, cilia, etc. and was used mainly in the
LM until SEM preps came along. Parducz is 6 parts of 2% osmium(aq) to 1
part sat'd HgCL2. (HgCl2 can be made by merely dumping in some HgCl2
into distilled water until it doesn't dissolve and go a bit further
until there is a small amt of ppt. on the bottom. We kept it in a brown
bottle and kept at room temp for many months. Take only from the top of
the bottle.) Mix it together just before use. Thus mix for instance 12
ml. of 2% aq Os and 2 ml HgCl2. We only used the mixture once and then
properly disposed of it. We often didn't bother with the glut as the
Parducz did the trick, but if we had to keep the cells before they were
prepared then we kept them in glut. (Something like a 3% glut in a
non-phosphate buffer. Phosphates ppt for SEM.)
Time: 1-2 hrs in glut (unless stored) and then 1 hr in Parducz then
either freeze drying or CPD after proper dehydration in EtOH. We never
went through amyl acetate. OR we fixed directly into Parducz for 1 hr
then dehydration and CPD or FD. (FD doesn't require dehydration and we
went directly from the Parducz - quickly froze it, then Pearse Tissue Dryer)

2. Also check how quickly your CPD is releasing pressure. If it is
more than 100 psi per minute then it is likely doing the damage.

3. Agree with the other dehydration comments.

Good Luck,

Judy


Judy Murphy, PhD
microscopyproducts
Stockton, CA


Michal.Jarnik-at-fccc.edu wrote:

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From: nbyers23-at-msn.com
Date: Sat, 3 Feb 2007 17:10:12 -0600
Subject: [Microscopy] AskAMicroscopist: how to properly draw to scale

Contents Retrieved from Microscopy Listserver Archives
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Below is the result of your feedback form (NJZFM-ultra-55). It was
submitted by (nbyers23-at-msn.com) from
http://microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on
Saturday, February 3, 2007 at 17:08:16
---------------------------------------------------------------------------

Email: nbyers23-at-msn.com
Name: Nanci Byers

Organization: Weber State University

Education: Undergraduate College

Location: Ogden, Utah USA

Question: I am trying to find some additional materials on learning
how to properly draw to scale when viewing slides through a
microscope. My professor went over the concept very quickly and I
would like some additional information. I am currently taking a
botany class.

Thanks for all your assistance!

---------------------------------------------------------------------------

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From: pbgrover-at-yahoo.com
Date: Sat, 3 Feb 2007 20:17:25 -0600
Subject: [Microscopy] Re: AskAMicroscopist: how to properly draw to scale

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear Nanci,

How refreshing to hear of a botany professor who still
teaches looking down a microscope tube and drawing!
It seems these days teachers prefer to interface a
digital camera to a microscope and broadcast it to
students' laptops or something. It takes me back to
undergrad school in the early 70s when my botany prof
taught us how to stipple. (I'm not just being
maudlin; I really think putting something on a slide
with your own hands and putting it under the lens and
focusing on it just makes it seem real). I guess that
when you say draw to scale you mean keeping
proportions accurate in two dimensions, in which case
you could use an eyepiece reticle grid and make your
drawing on a similar grid on your paper. If you're
wondering about how to figure out how many times
bigger your drawing is than the actual object, you
could put a specimen of a known size (say, a finely
graduated machinist's ruler or whatever) under the
lens, then look at it through the eyepiece while using
your other eye to look at another ruler placed on your
drawing surface, superimposing the images, then divide
the actual known dimension on the microscope stage
into the equivalent dimension on your paper to arrive
at the number of times your drawing is magnified. I
hope this makes sense, or that someone else can
explain it more succinctly or tell you a better
method. Cheers! And kudos to your teacher!

Paul Grover



--- nbyers23-at-msn.com wrote:

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} Email: nbyers23-at-msn.com
} Name: Nanci Byers
}
} Organization: Weber State University
}
} Education: Undergraduate College
}
} Location: Ogden, Utah USA
}
} Question: I am trying to find some additional
} materials on learning
} how to properly draw to scale when viewing slides
} through a
} microscope. My professor went over the concept very
} quickly and I
} would like some additional information. I am
} currently taking a
} botany class.
}
} Thanks for all your assistance!

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From: jbpawley-at-wisc.edu
Date: Sun, 4 Feb 2007 14:45:23 -0600
Subject: [Microscopy] Re: SEM of Zebra fish

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


--|
--|Dear Listers,
--|
--|I am about to prepare some zebra fish heads for SEM - to look at morphology
--|&
--|take some measurements. What do you think are the pros & cons of critical
--|point drying versus HMDS drying after fixation in GDA+PVP in SCB followed by
--|osmium+potassium ferrocyanide followed by staining in aqueous UA before
--|dehydration for small fish heads? I have had success with the HMDS drying
--|using cultured cells and insect tissue. The thermocirculator attached to my
--|CPD has problems - it is difficult to heat slowly enough so until I can
--|replace it I thought HMDS might be an alternative method.
--|
--|
--|Many thanks
--|Ursula
--|---------------
--|
--|Ursula J. Potter
--|Centre for Electron Optical Studies (CEOS)
--|Building 3 West 2.15
--|The University of Bath
--|Claverton Down
--|Bath BA2 7AY
--|UK
--|Tel: 01225 385651
--|Email: U.J.Potter-at-bath.ac.uk


Dear Ursula,

If you want to make dimensional measurements on dried tissue, I
strongly recommend that you take the time to read

Boyde, A, Maconnachie, E. (1979) Volume changes during preparation of
mouse embryonic tissue for scanning electron microscopy, Scanning
2:149-163

Boyde, A, Maconnachie, E, (1981) Morphological correlations with
dimensional change during SEM specimen preparation, Scanning Electron
Microsc. 1981, IV:27-34


These are the only two publications of which I am aware where the
authors actually made dimensional measurements of soft tissue
(embryos) as they proceeded through all the stages of fixation,
dehydration, and CPD or other drying.

The BEST they got was only 60% volume shrinkage (i.e., the final
volume was 40% of the live volume. This works out to be about 25%
linear shrinkage.).

This dirty secret often goes unremarked because, as tissue-culture
cells are tacked down to glass slides, the x-y dimensional are
stabilized by the glass. The thickness shrinks but that is harder to
measure.

Of course, I am sure that some tissues may be more robust but I can
also assure you that many are much more sensitive. And as I noted,
60% was the BEST they got. There were many ways to make it worse.

Freeze drying was better, especially if you kept the tissue frozen to
about -100deg C while you looked at it. but this has its own
problems, particularly those to do with ice crystals.

As many readers will likely find my claims "ridiculous," I do
encourage you to read the papers and then do you own tests.

Good luck!

Jim P.

--
**********************************************
Prof. James B. Pawley, Ph. 608-263-3147
Room 223, Zoology Research Building,
FAX 608-265-5315
1117 Johnson Ave., Madison, WI, 53706
JBPAWLEY-at-WISC.EDU
3D Microscopy of Living Cells Course, June 16-28, 2007, UBC, Vancouver Canada
Info: http:// www.3dcourse.ubc.ca/ Applications due by March 15, 2007
"If it ain't diffraction, it must be statistics." Anon.

==============================Original Headers==============================
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From: oshel1pe-at-cmich.edu
Date: Mon, 5 Feb 2007 07:32:34 -0600
Subject: [Microscopy] Re: SEM of Zebra fish

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Ursula,

Let me chime in here and completely agree with Jim. I have measured
shrinkage on the order of 50% to 60% in soft tissues, in this case
neuromast end organs from fish lateral lines. The original sizes were
measured both by simple measuring of the intact end organs in a light
microscope, and measuring the impressions the end organs made in
silicone casts of the lateral lines. These two measurements were in
almost exact agreement. The end organs as seen in the SEM were at
best 1/2 the size of the living and the "fixed-only", i.e., not
dehydrated, organs.
Further the shrinkage was *not* isometric. That is, the shrinkages
along X and Y axis imposed onto the end organs were different.
Keep in mind that tissues have mechanical properties, and these
properties depend on the histology of the tissue. E.g., what kind of
collagen does the tissue have? In what directions do the fibers run?
Shrinkage in the direction of the fibers will be different than
shrinkage in the direction orthogonal to the fiber direction. And
that's just the begining.
I don't know of any studies on such tissue properties and how they
affect dimensional changes. If there are such references, I would
very much appreciate it if they were posted to the list.
Best answer to measuring zebra heads: do all your measurements with a
light microscope. Make yourself a little jig, so the heads can all be
held in exactly the same set of positions so all the measurements
will be correct. Otherwise you'll have measurement errors caused by
slightly different tilts of the heads.
Do this before fixation. A rule of thumb used by fish ecologists,
back when I was doing marine ecology, was that all formalin-fixed
fish lengths were 10% too short. That might not be really true, but
the shrinkage was.

Phil

} --|
} --|Dear Listers,
} --|
} --|I am about to prepare some zebra fish heads for SEM - to look at morphology
} --|&
} --|take some measurements. What do you think are the pros & cons of critical
} --|point drying versus HMDS drying after fixation in GDA+PVP in SCB
} followed by
} --|osmium+potassium ferrocyanide followed by staining in aqueous UA before
} --|dehydration for small fish heads? I have had success with the HMDS drying
} --|using cultured cells and insect tissue. The thermocirculator attached to my
} --|CPD has problems - it is difficult to heat slowly enough so until I can
} --|replace it I thought HMDS might be an alternative method.
} --|
} --|
} --|Many thanks
} --|Ursula
} --|---------------
} --|
} --|Ursula J. Potter
} --|Centre for Electron Optical Studies (CEOS)
} --|Building 3 West 2.15
} --|The University of Bath
} --|Claverton Down
} --|Bath BA2 7AY
} --|UK
} --|Tel: 01225 385651
} --|Email: U.J.Potter-at-bath.ac.uk
}
}
} Dear Ursula,
}
} If you want to make dimensional measurements on dried tissue, I
} strongly recommend that you take the time to read
}
} Boyde, A, Maconnachie, E. (1979) Volume changes during preparation of
} mouse embryonic tissue for scanning electron microscopy, Scanning
} 2:149-163
}
} Boyde, A, Maconnachie, E, (1981) Morphological correlations with
} dimensional change during SEM specimen preparation, Scanning Electron
} Microsc. 1981, IV:27-34
}
}
} These are the only two publications of which I am aware where the
} authors actually made dimensional measurements of soft tissue
} (embryos) as they proceeded through all the stages of fixation,
} dehydration, and CPD or other drying.
}
} The BEST they got was only 60% volume shrinkage (i.e., the final
} volume was 40% of the live volume. This works out to be about 25%
} linear shrinkage.).
}
} This dirty secret often goes unremarked because, as tissue-culture
} cells are tacked down to glass slides, the x-y dimensional are
} stabilized by the glass. The thickness shrinks but that is harder to
} measure.
}
} Of course, I am sure that some tissues may be more robust but I can
} also assure you that many are much more sensitive. And as I noted,
} 60% was the BEST they got. There were many ways to make it worse.
}
} Freeze drying was better, especially if you kept the tissue frozen to
} about -100deg C while you looked at it. but this has its own
} problems, particularly those to do with ice crystals.
}
} As many readers will likely find my claims "ridiculous," I do
} encourage you to read the papers and then do you own tests.
}
} Good luck!
}
} Jim P.
}
} --
} **********************************************
} Prof. James B. Pawley, Ph. 608-263-3147
} Room 223, Zoology Research Building,
} FAX 608-265-5315
} 1117 Johnson Ave., Madison, WI, 53706
} JBPAWLEY-at-WISC.EDU
} 3D Microscopy of Living Cells Course, June 16-28, 2007, UBC, Vancouver Canada
} Info: http:// www.3dcourse.ubc.ca/ Applications due by March 15, 2007
} "If it ain't diffraction, it must be statistics." Anon.
--
Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
voice: (989) 774-3576
dept. fax: (989) 774-3462

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From: lcgould-at-med.cornell.edu
Date: Mon, 5 Feb 2007 08:49:36 -0600
Subject: [Microscopy] Re: SEM of cells in monolayer

Contents Retrieved from Microscopy Listserver Archives
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Michal,
I would differ only slightly with Phil's suggestions: since your
cells are on or in collagen, you will probably need to extend your
dehydrations: perhaps 2 changes at each concentration, starting at
50% or even 30% as Phil suggested. Collagen is an incredible sponge
and I've had a miserable time with it over the years.
Lee
--
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy & Histology Core Facility
Manager, Optical Microscopy Core Facility
Weill-Cornell Medical College

voice (212)746-6146
fax (212)746-8175
http://www.cornellcelldevbiology.org
http://www.cornellbiochem.org

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From: bigelow-at-engin.umich.edu
Date: Mon, 5 Feb 2007 15:19:12 -0600
Subject: [Microscopy] RE: Eye Floaters

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

I agree, any diminishing of visual acuity is indeed a disturbing
event for microscopists; however, I don't think age is necessarily a
primary factor involved in causing eye floaters. I am nearly 84
years old, and don't have any floaters (however I seem to recall
having a few sometime in the distant past). The only thing I can
think of that might be contributing to this is the fact that I take
large amounts of vitamins of all types, about half the amounts
recommended by Linus Pauling in his book How to Live Longer and Feel
Better, and have been doing so for more than 20 years.

My problem now seems to be the gradual development of cataracts,
which my doctor tells me is a process definitely related to aging. It
may be that the vitamins (especially vitamin C) are slowing this
precess down, but I'm afraid I'll eventually need to have the
cataracts removed.
--
Wilbur C. Bigelow, Professor Emeritus
Materials Sci. & Engr., Univ. of Michigan
Ann Arbor, Michigan 48109-2136
e-mail: bigelow-at-engin.umich.edu;
Fx:734-763-4788; Ph:734-975-0858
Address mail to: 2911 Whittier Court
Ann Arbor, MI 48104-6731

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From: MCarlyle-at-veeco.com
Date: Mon, 5 Feb 2007 15:34:37 -0600
Subject: [Microscopy] SPM - Abstract Submission Deadline for Seeing at the Nanoscale Conference

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

----Abstract deadline is February 16! -----

Only 11 days left to submit an abstract for Seeing at the Nanoscale, the
fifth annual scientific conference focusing on nanostructural imaging,
characterization, and modification using scanning probe microscopy (SPM)
and related techniques.

The conference location is Santa Barbara, California, June 24-27, 2007.
Sponsored by Veeco Instruments and the California NanoSystems Institute
(CNSI) at the University of California, Santa Barbara (UCSB), this
two-and-one-half day event includes technical presentations, a
nanotechnology poster contest, and a beach barbecue-Santa Barbara style.

Highlighted by Keynote speakers Angela Belcher and David Awschalom,
Seeing at the Nanoscale provides an optimum forum for "scientists to
speak to scientists" on a wide variety of nanotechnology topics with
technical sessions on:

Extending the Limits of SPM

Using AFM and Combined AFM-Optical Techniques to Probe Biological
Structures and Forces

Next Generation Materials and Polymer Systems

Beyond Topography: Measurement of Physical Properties at the Nanoscale
- Nanomechanical, Local Property, Electrical, Optical, Magnetic &
Thermal

Instruments and Probes - New Tools & Techniques for Nanoscience


To submit your abstract, review submission guidelines, and learn more
about the conference, visit www.veeco.com/nanoconference.

Take part as a presenter in the industry's most dynamic conference!

Veeco Instruments & CNSI



_________________________________
Marlene Carlyle
Conference Coordinator
Veeco Instruments
112 Robin Hill Road
Santa Barbara, CA 93117
Phone: 805-967-1400 Fax: 805-967-7717
mcarlyle-at-veeco.com
_________________________________




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From: mike_microscopy-at-yahoo.com
Date: Tue, 6 Feb 2007 01:42:36 -0600
Subject: [Microscopy] viaWWW: Looking for simulation software for CTEM image

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Email: mike_microscopy-at-yahoo.com
Name: Mike

Title-Subject: [Filtered] Looking for simulation software for CTEM image

Question: I am looking for Windows-based software that allows us to
simulate bright-field and dark-field images. The images to be
simulated are about the fringes observed at the interface with known
elastic strains. All atomic positions, including the atoms located on
both sides of the interface and the atoms
suffering from the elastic stains at the interface, and the indices
of the interface are available. The orientation of the crystals is
also known. In this way, the new unit cell involving the interface
and the atoms on both sides of the interface can be built.
I am a material researcher, not familiar with the principles used in
software. So hoping the software is of user friendly interface. It is
preferential that just inputting all atomic
coordinates relative to the new unit cell and giving the observed
orientation and the thickness of the specimen the bright-field and
dark-field images can be simulated and output. Please let me know
where to get such simulation software. Any input would be highly
appreciated!

---------------------------------------------------------------------------

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From: camiller-at-anatomy.iupui.edu
Date: Tue, 6 Feb 2007 01:43:20 -0600
Subject: [Microscopy] viaWWW: Joint LAS Spring Meeting

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Email: camiller-at-anatomy.iupui.edu
Name: Caroline Miller

Organization: Indiana Microscopy Society

Title-Subject: [Filtered] Joint LAS Spring Meeting

Question: There will be a joint LAS Spring Meeting, "Imaging for
Nanotechnology," hosted by the Indiana Microscopy Society in
Indianapolis, IN (home of the 2006 NFL Super Bowl Champions!)on April
20th and 21st, 2007. Co-sponsored by Central States, Iowa, Midwest
and Michigan LAS. Friday's session, April 20th will include five
speakers, Dr. M. Marko, Dr. W. Landis, R. Gursky, Dr. J. Pawley and
Dr. D. Newbury with an evening social at the "White River Gardens."
Saturday's session, April 21st will be a half day of workshops, demos
and tours of the Electron Microscoppy Center and the Indiana Center
for Biological Microscopy. Awards will be given for the best poster
either by a student or faculty/staff and for the best Biological and
Physical Science Micrograph. There is a discount if you pre-register
before March 15th. Abstract deadline is March 1st. Check out the INMS
web site for a complete program, registration form, accomodations and
application for a student fellowship. www.indianamicroscopy.org
Send your abstract or questions to the Program Chair, Caroline
Miller, camiller-at-anatomy.iupui.edu, fax # 317-278-2040.

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6, 12 -- From: camiller-at-anatomy.iupui.edu (by way of MicroscopyListserver)
6, 12 -- Subject: viaWWW: Joint LAS Spring Meeting
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From: rra-at-stowers-institute.org
Date: Tue, 6 Feb 2007 01:43:42 -0600
Subject: [Microscopy] viaWWW: Tousimis Samdri

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Email: rra-at-stowers-institute.org
Name: Rhonda Allen

Organization: Stowers Institute

Title-Subject: [Filtered] Tousimis Samdri

Question: Good Afternoon,

I am looking for a distributor of the Tousimis Samdri Critical Point
Dryer for the Kansas City, Missouri area.

Thanks,

Rhonda Allen

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From: cpetty1-at-umbc.edu
Date: Tue, 6 Feb 2007 16:43:41 -0600
Subject: [Microscopy] Electron Microscopy Mainteance Training

Contents Retrieved from Microscopy Listserver Archives
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A colleague of mine was looking to lower his costs on maintenance of his
Jeol SEM. He searched for an EM maintenance course and found the company
Protrain. We scheduled a 5 day training course with Stephen Chapman of
Protrain. and that was the best invest we ever made. Months before the
training was to begin we were asked what scopes we had and Steve then
tailored a course that he taught us at our site on our scopes. Steve
taught us normal scope maintenance and he taught us how to trouble shoot
not just the column but user induced problems. Mr. Chapman scripted
customized operating instructions for the TEM in our facilities as we
operated the microscope in addition to a digital book on Electron
Microscopy. We were also showed how to improve the safety of our
laboratories and core facility.

Don Johnson Physics Department University of Maryland Baltimore County

Chere Petty Department of Biological Sciences University of Maryland
Baltimore County

We have no financial interest in Protain.

--
Chere Petty, M.S.
Manager of Keith R. Porter Imaging Facility
Department of Biological Sciences
University of Maryland Baltimore County (UMBC)
1000 Hilltop Circle
Baltimore, Maryland 21250
Fax: 410-455-3875
Cell: 301-367-8408
cpetty1-at-umbc.edu
www.emumbc.com


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From: jzheng-at-uci.edu
Date: Tue, 6 Feb 2007 22:04:02 -0600
Subject: [Microscopy] Good opportunity for Materials Characterization Specialist

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Dear Materials Characterization Specialist:
Here is an open job at University of California. Please apply it by
following the instruction below. Thanks for your attention.
Jian-Guo



Materials Characterization Specialist
University of California, Irvine
Salary: Commensurate with experience

The University of California, Irvine is seeking a materials
characterization specialist to work in the campus-wide Nanomaterials
Characterization and Fabrication Facility (NCF2). The successful candidate
is expected to have an earned PhD degree in a relevant field, possess an
extensive knowledge in analytical instrumentation (SEM, XRD, AFM, TEM,
FTIR, TGA, DSC) and research implementation, and have rich experience in
sample preparation. He/she should have either extensive knowledge of
techniques and protocols in soft materials characterization, or
demonstrate a desire to acquire such knowledge. The applicant should also
have excellent writing and inter-personal communication skills, and strong
team spirit. Good computing skills are also desirable.
The materials characterization specialist's responsibilities include
carrying out day-to-day operations of analytical equipments including, but
not limited to, SEM, XRD, TEM and AFM/STM. He/she will also be responsible
for (1) maintaining all instruments in good working condition, (2)
training and helping users in the use of analytical equipments, (3)
preparing samples and providing help to users in sample preparation, (4)
assisting in courses related to analytical instrumentation, (5) conducting
service for off-campus and industrial users, (6) maintaining the facility
infrastructure, and (7) carrying out miscellaneous facility-related tasks
assigned by the facility director. The specialist will report to the
director of NCF2. Salary is commensurate with experience. This position
will open immediately and remain open until filled.

Please include in the application the following materials:
• Curriculum Vita
• 2-3 publications
• Three letters of recommendation letters (may be submitted shortly after
the submission of the application)

Ms. Dorothy Miles
4100 Calit2 Bldg
Irvine, CA 92697-2800
djmiles-at-uci.edu
ph: 949/824-5178
fax: 949/824-4403

The University of California, Irvine is an equal opportunity employer
committed to excellence through diversity.



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From: miranda.s.norby-at-sendit.nodak.edu
Date: Wed, 7 Feb 2007 01:11:45 -0600
Subject: [Microscopy] AskAMicroscopist: student microscopes

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Email: miranda.s.norby-at-sendit.nodak.edu
Name: Miranda Norby

Organization: Rolette Public School

Education: Graduate College

Location: Rolette, ND

Question: I teach 7-12 science-classes include Earth Science,
Biology, Chemistry, Physics. We are in the process of ordering
student microscopes. I was hoping to receice some recommendations as
to products that you have found to be student friendly and durable.
Thank you for any imput you can provide.

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From: acorn1800-at-yahoo.com
Date: Wed, 7 Feb 2007 01:13:12 -0600
Subject: [Microscopy] viaWWW: Nikon EFM microscope camera

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Email: acorn1800-at-yahoo.com
Name: Wayne

Title-Subject: [Filtered] Nikon EFM microscope camera

Question: I have recently aquired a Nikon Microscope Camera, model
EFM. I cannot find the battery compartment. I would also like a
copy of the operating manual, I can pay for copying and shipping
costs.

Thanks,
Wayne

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From: dlowry-at-asu.edu
Date: Wed, 7 Feb 2007 01:13:48 -0600
Subject: [Microscopy] viaWWW: critical point drying question

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Email: dlowry-at-asu.edu
Name: David Lowry

Organization: Arizona State University

Title-Subject: [Filtered] critical point drying question

Question: Is ethanol miscible in liquid CO2? I can't seem to find
anywhere this is explicitly stated. I have seen SEM protocols which
imply they use EtOH as dehydrating agent and then directly CPD with
CO2. We have an older Balzers CPD020 unit and the manual has a
flow-chart which shows only acetone or amyl acetate as dehydrants
prior to CO2 transition. It is my understanding that EtOH is a weaker
organic solvent than acetone, and if EtOH mixes with CO2, this may be
useful for certain CPD applications. Thanks

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From: David.Patton-at-uwe.ac.uk
Date: Wed, 7 Feb 2007 02:57:56 -0600
Subject: [Microscopy] viaWWW: critical point drying question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

We have used ethanol followed by liquid carbon dioxide for years in our
Tousimis Samdri-780 CPD. I switched from acetone, which I have been
told mixes better than ethanol with liquid carbon dioxide, to reduce
extraction from samples.

Dave

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Email: dlowry-at-asu.edu
Name: David Lowry

Organization: Arizona State University

Title-Subject: [Filtered] critical point drying question

Question: Is ethanol miscible in liquid CO2? I can't seem to find
anywhere this is explicitly stated. I have seen SEM protocols which
imply they use EtOH as dehydrating agent and then directly CPD with
CO2. We have an older Balzers CPD020 unit and the manual has a
flow-chart which shows only acetone or amyl acetate as dehydrants
prior to CO2 transition. It is my understanding that EtOH is a weaker
organic solvent than acetone, and if EtOH mixes with CO2, this may be
useful for certain CPD applications. Thanks

------------------------------------------------------------------------
---

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From: chrisj-at-hitachi-hitec-uk.com
Date: Wed, 7 Feb 2007 03:49:40 -0600
Subject: [Microscopy] Re: viaWWW: critical point drying question

Contents Retrieved from Microscopy Listserver Archives
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Dear David,

I routinely use acetone in a Balzers critical point drier but sometimes
want to dry samples that may be damaged by the acetone. In these cases (for
example preserving the outer surfaces of insect eggs prior to SEM
examination) I use ethanol. From my experience, the ethanol is nowhere near
as miscible as acetone in liquid CO2 but still works perfectly well.

You need to use more exchanges of the liquid CO2 in order to replace the
ethanol, leaving to stand for a few minutes between each exchange. If your
CPD system has a stirrer, even better! I find that the drying process in
the CPD with ethanol can take around four times longer than the
conventional technique using acetone. However as long as it achieves the
result, then the additional time has been worthwhile.

Best of luck!

Regards,

Chris

Chris Jones
-----------------------------------
Hitachi High-Technologies
7 Ivanhoe Road
Hogwood Industrial Estate
Finchampstead
Wokingham
Berks RG40 4QQ
Tel +44(0)118 932 8632
Fax +44(0)118 973 2622
chrisj-at-hitachi-hitec-uk.com
www.hitachi-hitec-uk.com


|---------+----------------------------}
| | dlowry-at-asu.edu |
| | |
| | 07/02/2007 07:20 |
| | Please respond to|
| | dlowry |
| | |
|---------+----------------------------}
} ------------------------------------------------------------------------------------------------------------------------------|
| |
| To: chrisj-at-hitachi-hitec-uk.com |
| cc: |
| Subject: [Microscopy] viaWWW: critical point drying question |
} ------------------------------------------------------------------------------------------------------------------------------|







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Email: dlowry-at-asu.edu
Name: David Lowry

Organization: Arizona State University

Title-Subject: [Filtered] critical point drying question

Question: Is ethanol miscible in liquid CO2? I can't seem to find
anywhere this is explicitly stated. I have seen SEM protocols which
imply they use EtOH as dehydrating agent and then directly CPD with
CO2. We have an older Balzers CPD020 unit and the manual has a
flow-chart which shows only acetone or amyl acetate as dehydrants
prior to CO2 transition. It is my understanding that EtOH is a weaker
organic solvent than acetone, and if EtOH mixes with CO2, this may be
useful for certain CPD applications. Thanks

---------------------------------------------------------------------------

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From: keith.morris-at-ucl.ac.uk
Date: Wed, 7 Feb 2007 06:56:49 -0600
Subject: [Microscopy] AskAMicroscopist: student microscopes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Miranda,

We get asked this fairly regularly by schools and parents (so we have stock
replies). Often professional microscopists aren't the best source of
information for cheap microscope's as all our optical systems are always
over £10,000, and most lab mainstays like our three confocal microscopes
come in at nearer £250,000 each (and users still complain about image
quality).

Cheap microscopes under £100 are always a disappointment and toy (often
called student) microscopes can be very poor. For home use or a
demonstration model, you can easily buy second-hand via ebay, but again
there are risks that the optics will be damaged or simply very dirty and
difficult to clean and you may make an expensive mistake. Look for old
branded 'laboratory' microscopes e.g. Bausch & Lomb, Prior, Leitz, Reichert,
Baker, but not Tasco toys. Famous existing brands like Zeiss and Olympus
attract a high premium. The sellers are often not microscopists though, and
many are sold as collector's items and not intended for 'scientific' use.
However many home users often get bored with new microscopes after a while,
so there is a second hand market for cheaper school/college lab grade
models. Also I would try any local microscope enthusiast clubs - they aren't
as common as the many excellent astronomy [telescope] clubs but they are
about and have knowledgeable enthusiasts with an eye for low cost quality
systems. To purchase for classes you will have to buy new, probably from a
schools supplier.

There are suppliers geared up to providing cheaper microscopes for schools &
colleges, so you can ask around at other school/college's science
departments, but expect to pay nearer £500 each for a quality setup
(although with those like bottom end Meade [www.meade.com] at around £100
you can see something at low mag (~20x objective i.e. around 180x mag) with
a quality stained section - try some on approval?.

For pond life etc. a stereoscopic 'dissecting' microscope (40x to 120x mag)
is ideal for home/school use. Also remember of course that you can get a
really long way with a good large magnifying glass (not the really small
hi-mag cheap folding lens ones, try before you buy) - I have a few excellent
ones at home for £1 and a good low mag Osram one that includes an
illuminating halogen bulb at £8. In general I would say a well made stereo
dissecting microscope is a good choice (if not the best) for younger kids as
it's great for viewing living things and enlarges what you can see already -
look for 40x rather than 4x though. Decent ones are a bit expensive (£500+).
These are ideal for small animals and things like colonies but are totally
unsuitable for viewing single cells or sections where something like 100x to
600x is preferred (expensive 20, 40 to 63x objectives), and requires a
standard compound microscope.

Generally prepared slides can rapidly get very boring for all ages, but
living or unusual things (even hamster fur) always attract an audience. Also
try your flatbed film scanner, (not the LiDe types that have a very limited
depth of focus, and any from around £60 upwards) that will be good for
looking at soft static things: leaves, fruit, nuts, household objects (scan
at max resolution and try reflected and 'film' mode). In the UK there are
sites like http://www.wedgwood-group.com/microscopes_digital.htm that cater
for schools and colleges, providing standard compound and stereo microscopes
as well as cheap PC video based microscope solutions where the whole class
of children can look at a computer screen with some pushing and shoving.
Best to try them on 'approval' as many cheap microscopes can be very
disappointing if you expect too much. For things like living
cells/microscopic organisms you would probably want some form on contrast
enhancement like Phase Contrast optics that adds to the complexity and
expense (and starts to put the microscope towards the £1,000 category).
Viewing mostly fixed & stained prepared sections obviously reduces the need
for any optical contrast enhancement.

Excellent pre-prepared stained slides of simple organisms, plant stems and
leaves or bits of rats, insects etc.. can be bought via ebay, but they tend
to be expensive and are easily broken by any age-group. Mounted slides keep
well though, so 'vintage' ones even from 50 years ago can still look OK.
Again school suppliers are geared up to provide for this sort of thing far
more cheaply (and most schools have an aging collection anyway).

At home and our Primary school (under 11) we use the Digital Blue QX-5
(£70)- it's fun but pretty useless for serious microscope work as it's so
low res (but at 640 x 480 better than the old Intel QX-3 it replaced). It
puts the image on a PC screen. I have one at home for my kids (boy 10 and
girl 12) but it only gets occasional use now the novelty has worn off. See
http://micro.magnet.fsu.edu/optics/intelplay/index.html (not updated for the
QX-5 but all applies - the site even discusses ways of contrast enhancement
etc..). Once on the PC the 640x480 images can be manipulated and pasted etc,
and the QX-5 does time-lapse for things like crystal growth. Living plants
growing and small animals. This toy has nowhere near the resolution you
require though. The similar but far better built Olympus MIC-D was great but
being over £500 it was just too expensive for most schools and is now
discontinued - there are other similar budget video microscope systems about
though.

The macro on a good digital camera (like the image stabilised 5MP Canon S2IS
- http://www.dpreview.com/reviews/canons2is)is also worth a try,
particularly with a small tripod and halogen bendy desk lamp, if very
close-up. I have an E500 digital SLR system with enlargement rings but they
are more difficult to use. You can get quite reasonable pictures by resting
a small compact digital camera lens against the eyepiece of a microscope.
Plus you can use the camera for normal photography when you get bored with
microscopy. I wouldn't fancy a large class using my E500 SLR though.

By the way, do try growing crystals on a slide, a few drops of a saturated
solution of salt (NaCl) or copper sulphate will grow superb crystals on the
surface of a slide when viewed under a microscope (but it takes a few hours
for the crystals to form and they often look best before the liquids all
gone). Just make sure you don't drive the objective tips into the solution.
It's not biology but its fun (see http://micro.magnet.fsu.edu/.

Sorry I can't offer more specific help as our microscopes are rather more
expensive than the one you wish to purchase.

Regards


Keith

Try looking in Amazon.com for decent microscope books with lots of pictures
- and they have a good customer review system for books and even microscopes
(often viewing micrographs in books or on the VDU screen is better than
trying to see it yourself down a microscope). Plus try web searches for
general sites like these (and for more specific topics) :

http://www.101science.com/Microscope.htm
http://micro.magnet.fsu.edu/
http://www.microscopy-uk.org.uk/index.html
http://www.btinternet.com/~stephen.durr/
http://www.mccroneatlas.com
http://www.diatoms.co.uk [for fun images made of diatoms]


Examples of sources for cheap school grade microscopes in the UK are:

http://www.brunelmicroscopes.co.uk/slide-sets.html
http://www.espmodels.co.uk/
http://www.technologysupplies.co.uk/
http://www.wedgwood-group.com/microscopes.htm (microscopes only)
http://www.wedgwood-group.com/microscopes_slides.htm (slides)

Have an internet search for school/lab suppliers in the US or just ask other
school science departments.

Our lab technicians dealt with all the ordering of prepared slides, we just
had to make a general request for the item. You can also buy on ebay but
that is probably more for collectors of science equipment and quality may
vary. However in the UK there are a lot of specialised suppliers of
scientific equipment for secondary schools, who supply prepared slides quite
cheaply. A small selection is:

http://www.brunelmicroscopes.co.uk/slide-sets.html
http://www.espmodels.co.uk/
http://www.technologysupplies.co.uk/
http://www.wedgwood-group.com/microscopes.htm (microscopes only)
http://www.wedgwood-group.com/microscopes_slides.htm (slides)

Again the US has many similar school suppliers.







-----Original Message-----
X-from: miranda.s.norby-at-sendit.nodak.edu
[mailto:miranda.s.norby-at-sendit.nodak.edu]
Sent: 07 February 2007 07:18
To: keith.morris-at-ucl.ac.uk

This Question was submitted to Ask-A-Microscopist by
(miranda.s.norby-at-sendit.nodak.edu)
from
http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html
on Tuesday, February 6, 2007 at 14:24:32
Remember to consider the Grade/Age of the student when considering the
Question
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Please reply to both miranda.s.norby-at-sendit.nodak.edu as well as
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---------------------------------------------------------------------------

Email: miranda.s.norby-at-sendit.nodak.edu
Name: Miranda Norby

Organization: Rolette Public School

Education: Graduate College

Location: Rolette, ND

Question: I teach 7-12 science-classes include Earth Science,
Biology, Chemistry, Physics. We are in the process of ordering
student microscopes. I was hoping to receice some recommendations as
to products that you have found to be student friendly and durable.
Thank you for any imput you can provide.

---------------------------------------------------------------------------

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From: kenconverse-at-qualityimages.biz
Date: Wed, 7 Feb 2007 07:41:20 -0600
Subject: [Microscopy] AskAMicroscopist: student microscopes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Miranda,
If she hasn't already replied to you, contact Caroline Schooly at
schooley-at-mcn.org
She is a wealth of information at the public school level.

Ken Converse
owner

QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
474 So. Bridgton Rd.
Bridgton, ME 04009
207-647-4348
Fax 207-647-2688
kenconverse-at-qualityimages.biz
qualityimages.biz



-----Original Message-----
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[mailto:miranda.s.norby-at-sendit.nodak.edu]
Sent: Wednesday, February 07, 2007 2:16 AM
To: kenconverse-at-qualityimages.biz

This Question was submitted to Ask-A-Microscopist by
(miranda.s.norby-at-sendit.nodak.edu)
from
http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html
on Tuesday, February 6, 2007 at 14:24:32
Remember to consider the Grade/Age of the student when considering the
Question
---------------------------------------------------------------------------
Please reply to both miranda.s.norby-at-sendit.nodak.edu as well as
to the Microscopy Listserver
---------------------------------------------------------------------------

Email: miranda.s.norby-at-sendit.nodak.edu
Name: Miranda Norby

Organization: Rolette Public School

Education: Graduate College

Location: Rolette, ND

Question: I teach 7-12 science-classes include Earth Science,
Biology, Chemistry, Physics. We are in the process of ordering
student microscopes. I was hoping to receice some recommendations as
to products that you have found to be student friendly and durable. Thank
you for any imput you can provide.

---------------------------------------------------------------------------

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From: mboucher-at-umn.edu
Date: Wed, 7 Feb 2007 07:49:55 -0600
Subject: [Microscopy] Cryo TEM operator needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

We are advertising for a cryo TEM operator for a new 300KV cryo TEM to be
delivered by early April, 2007.
Please see our ad on the MSA web site at:
http://www.microscopy.org/MSAUnits/PlacementOffice/JobListings.html

or go to the University of MN job site link for more details:
http://employment.umn.edu/applicants/Central?quickFind=58119

or you can contact me directly for more information.
Regards,
Mike
******************************************************************
Michael L. Boucher Sr. mboucher-at-tc.umn.edu
Lab Manager Rm 18 Office Ph 612-624-6590
I.T. Characterization Facility Rm 12 Main Off Ph 612-626-7594
MiNTeC node of the National Nanotechnology Infrastructure Network

University of MN Fax 612-625-5368
12 Shepherd Labs
100 Union Street S.E.
Minneapolis, MN 55455 http://www.charfac.umn.edu
********************************************************************



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From: mboucher-at-umn.edu
Date: Wed, 7 Feb 2007 08:20:40 -0600
Subject: [Microscopy] TEM sample preparation technician needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

We are advertising for a TEM sample preparation technician at the UMN
Characterization Facility. This position's emphasis will be on bio-medical
sample prep experience and microtomy rather than prep of hard materials.
Please see our ad at the University of MN job site link for details:
http://employment.umn.edu/applicants/Central?quickFind=59448

or you can contact me directly for more information.

Mike
******************************************************************
Michael L. Boucher Sr. mboucher-at-tc.umn.edu
Lab Manager Rm 18 Office Ph 612-624-6590
I.T. Characterization Facility Rm 12 Main Off Ph 612-626-7594
MiNTeC node of the National Nanotechnology Infrastructure Network

University of MN Fax 612-625-5368
12 Shepherd Labs
100 Union Street S.E.
Minneapolis, MN 55455 http://www.charfac.umn.edu
********************************************************************



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From: bingber-at-srrc.ars.usda.gov
Date: Wed, 7 Feb 2007 09:08:08 -0600
Subject: [Microscopy] viaWWW: critical point drying question

Contents Retrieved from Microscopy Listserver Archives
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I've also used ethanol as the transfer fluid with liquid CO2 for many years. Both Polaron and Ladd CPD's have been used without any problems. I tried amyl acetate for awhile but wasn't too fond of it. It's only advantage was being able to smell minute residues of it during the CPD flushing process.

However, the number of flushes of liquid CO2 and length of time between flushes depends on the size or thickness of your sample. Most of the ethanol is removed in the first few flushes. You really have to become familar with your samples to determine the time required to remove all of the ethanol. And as usual add a few flushes for good measure.

I've found certain arthrpods/insects etc. to be most difficult using CPD. The hard exoskeleton or cuticle allows ethanol to enter the body of the organism but doesn't easily allow the exchange of ethanol with liquid CO2. Tardigrades were especially aggravating. Alternative drying techniques than CPD with arthropods and insects are welcome.

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Dear David,

I routinely use acetone in a Balzers critical point drier but sometimes
want to dry samples that may be damaged by the acetone. In these cases (for
example preserving the outer surfaces of insect eggs prior to SEM
examination) I use ethanol. From my experience, the ethanol is nowhere near
as miscible as acetone in liquid CO2 but still works perfectly well.

You need to use more exchanges of the liquid CO2 in order to replace the
ethanol, leaving to stand for a few minutes between each exchange. If your
CPD system has a stirrer, even better! I find that the drying process in
the CPD with ethanol can take around four times longer than the
conventional technique using acetone. However as long as it achieves the
result, then the additional time has been worthwhile.

Best of luck!

Regards,

Chris

Chris Jones
-----------------------------------
Hitachi High-Technologies
7 Ivanhoe Road
Hogwood Industrial Estate
Finchampstead
Wokingham
Berks RG40 4QQ
Tel +44(0)118 932 8632
Fax +44(0)118 973 2622
chrisj-at-hitachi-hitec-uk.com
www.hitachi-hitec-uk.com


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We have used ethanol followed by liquid carbon dioxide for years in our
Tousimis Samdri-780 CPD. I switched from acetone, which I have been
told mixes better than ethanol with liquid carbon dioxide, to reduce
extraction from samples.

Dave

-----Original Message-----
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Sent: 07 February 2007 07:18
To: David Patton

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Email: dlowry-at-asu.edu
Name: David Lowry

Organization: Arizona State University

Title-Subject: [Filtered] critical point drying question

Question: Is ethanol miscible in liquid CO2? I can't seem to find
anywhere this is explicitly stated. I have seen SEM protocols which
imply they use EtOH as dehydrating agent and then directly CPD with
CO2. We have an older Balzers CPD020 unit and the manual has a
flow-chart which shows only acetone or amyl acetate as dehydrants
prior to CO2 transition. It is my understanding that EtOH is a weaker
organic solvent than acetone, and if EtOH mixes with CO2, this may be
useful for certain CPD applications. Thanks

------------------------------------------------------------------------
---


Bruce F. Ingber, Biologist
Electron Microscopy
USDA-ARS, SRRC
CSQ-EM
1100 Robert E. Lee Blvd.
New Orleans, LA 70124
bingber-at-srrc.ars.usda.gov
bingber46-at-hotmail.com
504-286-4270 desk phone
504-782-6323 cell


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From: bfoster-at-mme1.com
Date: Wed, 7 Feb 2007 09:30:18 -0600
Subject: [Microscopy] Re: AskAMicroscopist: student microscopes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi, Miranda

At the risk of being a little commercial, I'd also recommend getting
a copy of our book, Optimizing Light MIcroscopy for Biological and
Clinical labs. It has over 80 mini experiments that you might find
helpful in your classroom.

Hope this was helpful,
Barbara Foster

Microscopy/Microscopy Education
313 S Jupiter Rd, Suite 100
Allen, TX 75002
P: 972-954-8011
W: www.MicroscopyEducation.com


MME is now scheduling customized, on-site courses through July. Call
us today for details.

P. S.
Need a good general reference or light microscopy text for the Spring
semester? Call us today to learn more about "Optimizing LIght
Microscopy". Copies still available through MME... even for
class-room lots ... and we give quantity discounts. Call Ken Piel at
(972)954-8011.


At 09:24 AM 2/7/2007, miranda.s.norby-at-sendit.nodak.edu wrote:



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From: fcmalhao-at-icbas.up.pt
Date: Wed, 7 Feb 2007 12:00:09 -0600
Subject: [Microscopy] viaWWW: PVP

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Email: fcmalhao-at-icbas.up.pt
Name: Fernanda Malh“o

Organization: ICBAS

Title-Subject: [Filtered] PVP

Question: Hi everybody!

Does anyone have any explanation for what is the
function of PVP(polyvinylpyrrodine) in the
phosphate buffer for fixing perfusion?
I have seen it in an article and now I am curious.
Thanks in advance.

---------------------------------------------------------------------------


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From: martin.roe-at-nottingham.ac.uk
Date: Wed, 7 Feb 2007 12:00:44 -0600
Subject: [Microscopy] viaWWW: EDX detectors for JEOL 4000FX TEM

Contents Retrieved from Microscopy Listserver Archives
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Email: martin.roe-at-nottingham.ac.uk
Name: Martin Roe

Organization: Nottingham University

Title-Subject: [Filtered] EDX detectors for JEOL 4000FX TEM

Question: Hello listservers,
Does anyone have an old/spare high angle EDX detector (preferably a
LINK/ OI) for a JEOL 4000FX TEM?
Secondly, does anyone happen to know if an EDX detector was ever made
suitable for use on the same microscope's horizontal port (on
objective lens) i.e. a horizontally mounted detector.
Thanks
Martin Roe


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From: schooley-at-mcn.org
Date: Wed, 7 Feb 2007 12:24:35 -0600
Subject: [Microscopy] Re: AskAMicroscopist: student microscopes

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Miranda -

Please look at the "buying school microscopes" section of the Project
MICRO web page (URL below) for detailed general advice. Since you
teach a wide grade range, be sure to get both dissecting and compound
scopes. And since this isn't the best of all possible worlds, you'll
need to control price. DON'T do that by buying used equipment on
eBay; research-grade scopes that get to that marketplace are too
complex for young students to use correctly, and they usually have
condition problems. Don't buy 100x objectives on the compound
scopes; they require immersion oil and an adjustable condenser, which
students won't use properly. That said, look for monocular compound
scopes with 4-10-40x objectives, fixed condenser & rechargable LED
illumination; you'll find remarkably good imports for around $150.
Durable monocular dissecting scopes are $70, and there's a great
portable scope for field trips for the same price. I'll be happy to
look at web listings of your tentative selections, and to answer
further questions; since I'm a retiree, I promise to be prompt.

Listers: If you care enough about this topic to have read this far,
I've probably horrified some of you. Please visit the MICRO booth at
M&M '07 in Ft. Lauderdale to see some of these scopes!

Caroline
--
Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
Box 117, 45301 Caspar Point Road
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.microscopy.org/ProjectMICRO

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From: delannoy-at-jhmi.edu
Date: Wed, 7 Feb 2007 14:10:46 -0600
Subject: [Microscopy] ultracut for sale

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

We have a Riechert Ultracut for sale, still
operational. Interested parties please
contact me for pricing,

M Delannoy
410 955-1365

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From: bigelow-at-engin.umich.edu
Date: Wed, 7 Feb 2007 14:55:51 -0600
Subject: [Microscopy] RE: Floaters and cataracts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Several people have written to me about my comments on floaters and
cataracts, and so I gather there is some general interest in this
subject. Therefore, I would like to pass along some comments made by
Linus Pauling about these matters in his book "How To Live Longer and
Feel Better' (ISBN 0-7167-1775-1).

In Chapter 23 he cites research results that make the following
points relating to the eyes:
The concentration of vitamin C in the aqueous humor of the
eye is twenty five times as high as in blood plasma (suggesting that
taking significant amounts of C might be beneficial to the quality of
this fluid)
Many investigators have reported that there is very little
vitamin C in the aqueous humor of eyes that have cataracts, and that
patients with them usually have low concentrations in their blood
plasma.
High intakes of vitamins C and E and B12 appear to be
beneficial in preventing senile cataracts.
Regular intakes of high doses of vitamins C also appears to
be beneficial in preventing and controlling glaucoma.

Pauling doesn't mention floaters specifically, but it would seem
reasonable to expect that anything that would improve the quality of
the fluid in the eye might also reduce the occurrence of these pesky
inclusions.

Pauling recommends a daily intake of 6 to 18 gm of vitamin C, 400 to
1600 IU of vitamin E, and one or two Super-B 50 tablets, plus a
mineral supplement. I haven't taken vitamins at the full level he
recommends, but have been taking 2 gm of C, 400 IU of E, and one
Super-B 50, plus a theraputic vitamin+mineral tablet, daily for the
past 20 years and think doing so has been very beneficial in many
ways.

--
Wilbur C. Bigelow, Professor Emeritus
Materials Sci. & Engr., Univ. of Michigan
Ann Arbor, Michigan 48109-2136
e-mail: bigelow-at-engin.umich.edu;
Fx:734-763-4788; Ph:734-975-0858
Address mail to: 2911 Whittier Court
Ann Arbor, MI 48104-6731

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5, 15 -- Subject: [Microscopy] RE: Floaters and cataracts
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From: johnf-at-geology.wisc.edu
Date: Wed, 7 Feb 2007 15:07:22 -0600
Subject: [Microscopy] cleaning apertures chemically?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Does anyone have any experience cleaning the carbonaeous gunk off of
Mo or Pt-Ir apertures with a solvent? I know about heating up in a Mo
etc boat, but looking for alternatives. Will ammonium hydroxide do
the trick? Or something else?

thanks.
--
========================================================
John Fournelle, Ph.D. office: (608) 262-7964 cell: (608) 438-7480
Cameron Electron Microprobe Lab lab: (608) 265-4798
Dept of Geology & Geophysics fax: (608) 262-0693
University of Wisconsin home: (608) 274-2245
1215 West Dayton St. email: johnf-at-geology.wisc.edu
Madison, WI 53706 amateur radio: WA3BTA
Personal http://www.geology.wisc.edu/~johnf/
Probe lab http://www.geology.wisc.edu/~johnf/sx51.html
Probe Sign Up Calender:
http://www.microscopy.wisc.edu/cgi-bin/calendar/microprobe/calendar.cgi

"The first rule of all intelligent tinkering is to save every cog and
wheel." -- Aldo Leopold

"For a successful technology, reality must take precedence over
public relations, for Nature cannot be fooled." -- Richard P.
Feynman

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From: kenconverse-at-qualityimages.biz
Date: Wed, 7 Feb 2007 21:03:14 -0600
Subject: [Microscopy] cleaning apertures chemically?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

John,
I've had very good luck for the last 30 years or so cleaning apertures with
a cut knap polishing cloth and 1 micron diamond paste. Just place the
aperture on the cloth with a little paste and put your finger on it and rub
it in a circular motion. Do both sides. Clean ultrasonically in Joy
dishwashing liquid and hot water, rinse in hot tap water or distilled water
and immediately blow dry with a duster to avoid water spots. My
understanding about Joy is that the Proctor & Gamble labs use it for
cleaning critical parts of AAUs and can find no residue. Apparently this is
not true of all dishwashing liquids.

This works with 1 mil foil (including multi-hole strips), 5 mil countersunk
and even the little Siemems apertures that are heavily countersunk. Just
don't try it with gold foil self-cleaning apertures. The gold foil is far
too thin and fragile for this technique.

Chuck Garber at SPI tells me that most metallographic diamond pastes have
silicones in them. That's a problem, but his pastes don't. As a bonus, the
SPI prices are very good. No financial interest, just a happy user.

As you are probably aware, heating moly in a platinum boat is a problem and
even heating Pt has limited usefulness because the Pt recrystalizes and
eventually you have an aperture with alligator skin and it won't work any
more (high astigmatism). Polishing a ruined Pt aperture will restore it,
probably due to the smearing of the metal by the diamond particles.

All in all it's pretty good because you don't need to know what the aperture
is made of, there's no complicated or exotic equipment needed (beyond an
ultrasonic cleaner), no organic solvents or other nasty stuff, and you can
use the same apertures for years. Once in a great while I might fold a 1
mil aperture, but that doesn't happen very often.

Ken Converse
Owner

QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
474 So. Bridgton Rd.
Bridgton, ME 04009
207-647-4348
Fax 207-647-2688
kenconverse-at-qualityimages.biz
qualityimages.biz



-----Original Message-----
X-from: johnf-at-geology.wisc.edu [mailto:johnf-at-geology.wisc.edu]
Sent: Wednesday, February 07, 2007 4:11 PM
To: kenconverse-at-qualityimages.biz

Does anyone have any experience cleaning the carbonaeous gunk off of
Mo or Pt-Ir apertures with a solvent? I know about heating up in a Mo
etc boat, but looking for alternatives. Will ammonium hydroxide do
the trick? Or something else?

thanks.
--
========================================================
John Fournelle, Ph.D. office: (608) 262-7964 cell: (608) 438-7480
Cameron Electron Microprobe Lab lab: (608) 265-4798
Dept of Geology & Geophysics fax: (608) 262-0693
University of Wisconsin home: (608) 274-2245
1215 West Dayton St. email: johnf-at-geology.wisc.edu
Madison, WI 53706 amateur radio: WA3BTA
Personal http://www.geology.wisc.edu/~johnf/
Probe lab http://www.geology.wisc.edu/~johnf/sx51.html
Probe Sign Up Calender:
http://www.microscopy.wisc.edu/cgi-bin/calendar/microprobe/calendar.cgi

"The first rule of all intelligent tinkering is to save every cog and
wheel." -- Aldo Leopold

"For a successful technology, reality must take precedence over
public relations, for Nature cannot be fooled." -- Richard P.
Feynman

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_________________________________________________________________
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From: frank.karl-at-degussa.com
Date: Thu, 8 Feb 2007 08:21:23 -0600
Subject: [Microscopy] Question about sulfate interference in FE spot test

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Let me float a question out into E-space about microchemical/spot test. My
limited experience suggest that high levels of sulfate (SO4) interferes or
reduces the sensitivity with Chamot's test for iron. Specifically the
potassium mercuric thiocyanate, potassium thiocyanate and the potassium
ferrocyanide test. Has anyone else noticed this problem?

I solved it by repetitively dry boiling my sample in dilute (15%) HNO3.
I'm just looking for confirmation.......

Stay safe...................Frank


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From: trent-at-ornl.gov
Date: Thu, 8 Feb 2007 10:04:14 -0600
Subject: [Microscopy] Cryo ultramicrotomy -help!

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

I have just recently begun using the Leica EM FCS cryo set-up for our
ultramicrotome. I'm having a little difficulty and was hoping that members
of this list could clue me into some tricks of the trade.
I am presently trying to section polymer samples and I need them to be
around 50nm in thickness. During cutting, the sections often curl or fold
like an accordion. So I pick them up with an eyelash tool and try (with much
difficulty and cold fingers) to unfold and stretch them out onto a copper
grid. The sections have no affinity for the Cu grid and are much happier
adhering to the eyelash or wadding up into an unusable lump. Short sections
don't work any better, since these just tend to flip away. I'm using a
anti-static device but it's difficult to place it into the correct position
since I'm using it's holder to hold the copper grids close to the diamond.

Thank you for any advice!
Shawn


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From: lesley.bechtold-at-jax.org
Date: Thu, 8 Feb 2007 10:31:59 -0600
Subject: [Microscopy] portable darkrooms

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

If anyone has any information on stand-alone darkrooms, I would appreciate hearing from you. Thank you.

Lesley


Lesley S. Bechtold
Senior Manager, Histopathology & Microscopy Sciences
The Jackson Laboratory
600 Main St.
Bar Harbor, ME 04609
207-288-6322



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From: tbargar-at-unmc.edu
Date: Thu, 8 Feb 2007 10:42:53 -0600
Subject: [Microscopy] Digital cameras used in electron tomography

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Are there any technical difficulties with using a side mounted digital
camera system versus a bottom mounted camera system for acquiring a tilt
series of images for 3D-reconstruction? Please respond privately.

Tom Bargar
University of Nebraska Medical Center
Core Electron Microscopy Research Facility
986395 Nebraska Medical Center
Omaha, NE 68198-6395
402-559-7347
tbargar-at-unmc.edu


==============================Original Headers==============================
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From: tbargar-at-unmc.edu
Date: Thu, 8 Feb 2007 10:54:07 -0600
Subject: [Microscopy] 120Kv TEM's used in electron tomography

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


We are working toward acquiring a 120Kv TEM with electron tomography
capability. I would like to hear from anyone who has an instrument (of any
brand) at this level. I would like to know your experiences with whatever
manufacturer's system you chose. Please, respond privately.

Tom Bargar
University of Nebraska Medical Center
Core Electron Microscopy Research Facility
986395 Nebraska Medical Center
Omaha, NE 68198-6395
402-559-7347
tbargar-at-unmc.edu


==============================Original Headers==============================
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From: TindallR-at-missouri.edu
Date: Thu, 8 Feb 2007 10:57:40 -0600
Subject: [Microscopy] Electron Microscopy Antique Road Show

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listers,

We are clearing rooms for a couple new scope installations and one gem
we turned up is a boxed set of filmstrips and cassette tapes by Crang
and Ward, entitled "Electron Microscopy: Principles and Practices",
copyright 1975 by the W.B. Saunders Company, Philadelphia, London,
Toronto. The box shows some wear, but the filmstrips and cassettes
appear to be complete and pristine, with their orignal patina. Some
color fade due to age.

Topics covered are: 1) The TEM, 2) Fixation and Embedment of Biological
Tissues, 3) Ultramicrotomy, 4) Ultrastructural Localization of Enzime
Activity, 5) Electron Microscope Autoradiography, 6) Freeze-etching, 7)
The High Voltage Electron Microscope, 8) The SEM, 9) Specimen
Preparation for SEM, and 10) X-ray Microanalysis.

At auction, a conservative estimate of value would be whatever emotional
attachment someone might attach to them. That's a conservative
estimate. Actual value could go even higher.

Any takers for cost of shipping?

Cheers,
Randy

Randy Tindall
Senior EM Specialist
Electron Microscopy Core Facility---We Do Small Well!
W125 Veterinary Medicine
University of Missouri
Columbia, MO 65211
Tel: (573) 882-8304
Fax: (573) 884-2227
Email: tindallr-at-missouri.edu
Web: http://www.emc.missouri.edu
On-line calendar:
http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount=
Week&NavType=Both&Type=TimePlan


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8, 23 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu}
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From: krensing-at-ucalgary.ca
Date: Thu, 8 Feb 2007 11:01:25 -0600
Subject: [Microscopy] Re: Digital cameras used in electron tomography

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

I would be interested in the feedback on this. Could people post to the
listserver?
Kim

tbargar-at-unmc.edu wrote:
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}
} Are there any technical difficulties with using a side mounted digital
} camera system versus a bottom mounted camera system for acquiring a tilt
} series of images for 3D-reconstruction? Please respond privately.
}
} Tom Bargar
} University of Nebraska Medical Center
} Core Electron Microscopy Research Facility
} 986395 Nebraska Medical Center
} Omaha, NE 68198-6395
} 402-559-7347
} tbargar-at-unmc.edu
}
}

}

--
Kim Rensing Ph.D.
Research Assistant Professor
Manager, Microscopy and Imaging Facility

University of Calgary
Health Sciences Centre
B129 - 3330 Hospital Drive NW
Calgary, AB, Canada T2N 4N1
403-220-3488
krensing-at-ucalgary.ca

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5, 22 -- Date: Thu, 08 Feb 2007 10:01:23 -0700
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From: ahlst007-at-umn.edu
Date: Thu, 8 Feb 2007 11:42:11 -0600
Subject: [Microscopy] Re: Cryo ultramicrotomy -help!

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Shawn,

You can get folding grids that look like two
regular mesh grids attached along one side,
which is the "hinge". You collect a section on
one side, then fold other side over on top so
section is clamped in place mechanically, so no
adhesion of section to grid takes place. This
would also tend to flatten the section to some
extent.

Most EM vendors that sell grids would have them.

Whether these folding grids are compatible with
requirements for collecting and observing
cryosections, I don't know, but it might be
worth a try.

Just my 2.5 cents worth...

Good luck,

Gib
Gilbert (Gib) Ahlstrand, Electron Microscopist,
Imaging Center, University of Minnesota
123 Snyder Hall
St. Paul, MN 55108
http://www.cbs.umn.edu/ic


trent-at-ornl.gov wrote:
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}
} I have just recently begun using the Leica EM FCS cryo set-up for our
} ultramicrotome. I'm having a little difficulty and was hoping that members
} of this list could clue me into some tricks of the trade.
} I am presently trying to section polymer samples and I need them to be
} around 50nm in thickness. During cutting, the sections often curl or fold
} like an accordion. So I pick them up with an eyelash tool and try (with much
} difficulty and cold fingers) to unfold and stretch them out onto a copper
} grid. The sections have no affinity for the Cu grid and are much happier
} adhering to the eyelash or wadding up into an unusable lump. Short sections
} don't work any better, since these just tend to flip away. I'm using a
} anti-static device but it's difficult to place it into the correct position
} since I'm using it's holder to hold the copper grids close to the diamond.
}
} Thank you for any advice!
} Shawn
--

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9, 21 -- From ahlst007-at-umn.edu Thu Feb