James, Have you looked at "operating microscopes"? Although binocular (stereo), they generally have very long working distances. I have an old Zeiss dissecting scope with a focal length in the range of 200mm. I love it, although there are certainly issues on resolution at 40X. I mostly use it at 6X.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: james99-at-uab.edu [mailto:james99-at-uab.edu] Sent: Monday, December 31, 2007 11:47 AM To: kenconverse-at-qualityimages.biz
This Question was submitted to Ask-A-Microscopist by (james99-at-uab.edu) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Wednesday, December 19, 2007 at 13:52:08 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both james99-at-uab.edu as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Email: james99-at-uab.edu Name: James Borham
Organization: University of Alabama at Birmingham
Education: Undergraduate College
Location: Birmingham, Alabama, United States of America
Title: Finding a Specialized Microscope
Question: I'm trying to find a new microscope with some specialized features, and I'm having a lot of trouble. I'm hoping one of you may be able to help me out. The feature that is needed most, and has been impossible to find, is a non-stereo microscope and/or lens with a parfocalizing distance of 90 mm or more. Do you have any idea where I could find such a thing? Is a device with such a long parfocalizing distance even classified as a microscope? I would appreciate any help on this problem.
==============================Original Headers============================== 8, 31 -- From zaluzec-at-microscopy.com Mon Dec 31 10:43:47 2007 8, 31 -- Received: from cubert.e-centre.net (morbo.e-centre.net [66.154.82.3]) 8, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id lBVGhlg5000383 8, 31 -- for {microscopy-at-microscopy.com} ; Mon, 31 Dec 2007 10:43:47 -0600 8, 31 -- Received: from [10.3.1.19] (helo=barracuda2.stayonline.net) 8, 31 -- by cubert.e-centre.net with esmtp (Exim 4.50) 8, 31 -- id 1J9Njt-0003Pf-OC 8, 31 -- for microscopy-at-microscopy.com; Mon, 31 Dec 2007 11:43:46 -0500 8, 31 -- X-ASG-Debug-ID: 1199119423-31474-157-0 8, 31 -- X-Barracuda-URL: http://10.3.1.19:8000/cgi-bin/mark.cgi 8, 31 -- Received: from et-lax-21.site.stayonline.net (unknown [74.8.222.131]) 8, 31 -- by barracuda2.stayonline.net (Spam Firewall) with ESMTP id 7472B198715F 8, 31 -- for {microscopy-at-microscopy.com} ; Mon, 31 Dec 2007 11:43:43 -0500 (EST) 8, 31 -- Received: from [172.16.7.135] ([172.16.7.135]) 8, 31 -- by et-lax-21.site.stayonline.net (8.13.8/8.12.6) with ESMTP id lBVGhgsN025334 8, 31 -- for {microscopy-at-microscopy.com} ; Mon, 31 Dec 2007 16:43:42 GMT 8, 31 -- Mime-Version: 1.0 8, 31 -- Message-Id: {p06240805c39ecc1cefb9-at-[172.16.7.135]} 8, 31 -- Date: Mon, 31 Dec 2007 10:41:26 -0600 8, 31 -- To: microscopy-at-microscopy.com 8, 31 -- From: james99-at-uab.edu (by way of MicroscopyListserver) 8, 31 -- X-ASG-Orig-Subj: AskAMicroscopist: Finding a Specialized Microscope 8, 31 --
Analytical Scientist/Electron Microscopist
The University of Wisconsin Eau Claire is seeking applicants for an Analytical Scientist/Electron Microscopist in the Materials Science Center, an interdisciplinary analytical facility, specializing in materials characterization. This is a full-time professional academic staff position beginning as early as July 1st 2008. Potential applicants may obtain a complete position description and application requirements at www.uwec.edu/Matsci/EMposition.html. Women, minorities, individuals with disabilities and veterans are encouraged to apply. The University is responsive to the needs of dual career couples. Criminal background checks are required prior to employment.
------------------------------------------------------------------------ Dr. Doug Dunham Director, Materials Science Center University of Wisconsin Eau Claire 105 Garfield Avenue Eau Claire, WI 54701 715-836-5312 fax: 715-836-3955 dunhamdj-at-uwec.edu
==============================Original Headers============================== 9, 25 -- From DUNHAMDJ-at-uwec.edu Wed Jan 2 11:09:00 2008 9, 25 -- Received: from exch07.uwec.edu (exch07.uwec.edu [137.28.1.180]) 9, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m02H8xqc001540 9, 25 -- for {microscopy-at-microscopy.com} ; Wed, 2 Jan 2008 11:08:59 -0600 9, 25 -- Received: from exch06.uwec.edu (137.28.1.171) by exch07.uwec.edu 9, 25 -- (137.28.1.180) with Microsoft SMTP Server (TLS) id 8.1.240.5; Wed, 2 Jan 2008 9, 25 -- 11:08:59 -0600 9, 25 -- Received: from CHERRYCOKE.uwec.edu ([172.28.1.61]) by exch06.uwec.edu 9, 25 -- ([137.28.1.171]) with mapi; Wed, 2 Jan 2008 11:08:59 -0600 9, 25 -- From: "Dunham, Douglas J." {DUNHAMDJ-at-uwec.edu} 9, 25 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 9, 25 -- Date: Wed, 2 Jan 2008 11:08:58 -0600 9, 25 -- Subject: Electron Microscopist Position 9, 25 -- Thread-Topic: Electron Microscopist Position 9, 25 -- Thread-Index: AchNYinSUpKjC6dCQF2sw1zt9VpmMA== 9, 25 -- Message-ID: {EEA4CA65D05DC54E874A89D9F51868193CC436717A-at-CHERRYCOKE.uwec.edu} 9, 25 -- Accept-Language: en-US 9, 25 -- Content-Language: en-US 9, 25 -- X-MS-Has-Attach: 9, 25 -- X-MS-TNEF-Correlator: 9, 25 -- acceptlanguage: en-US 9, 25 -- Content-Type: text/plain; charset="us-ascii" 9, 25 -- MIME-Version: 1.0 9, 25 -- Content-Transfer-Encoding: 8bit 9, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m02H8xqc001540 ==============================End of - Headers==============================
This looks just like a system I saw at Harvard in May 1994 for imaging lung with asbestos or other fibers for 3D reconstruction using VoxelView running on an SGI.
-mc
____________________________________________________________________________ Michael Cammer Analytical Imaging Facility Albert Einstein Coll. of Med. URL: http://www.aecom.yu.edu/aif/
==============================Original Headers============================== 7, 30 -- From cammer-at-aecom.yu.edu Wed Jan 2 13:48:24 2008 7, 30 -- Received: from mx1.aecom.yu.edu (mx1.aecom.yu.edu [129.98.1.51]) 7, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m02JmOa9017427 7, 30 -- for {microscopy-at-microscopy.com} ; Wed, 2 Jan 2008 13:48:24 -0600 7, 30 -- Received: from draco.aecom.yu.edu (draco.aecom.yu.edu [129.98.1.160]) 7, 30 -- by mx1.aecom.yu.edu (Postfix) with ESMTP id 8C5F69F0346; 7, 30 -- Wed, 2 Jan 2008 14:48:24 -0500 (EST) 7, 30 -- Received: from draco.aecom.yu.edu (unknown [127.0.0.1]) 7, 30 -- by draco.aecom.yu.edu (Symantec Mail Security) with ESMTP id 69C3E8B4006; 7, 30 -- Wed, 2 Jan 2008 14:48:24 -0500 (EST) 7, 30 -- X-AuditID: 816201a0-a80bdbb000006615-ab-477bea88354d 7, 30 -- Received: from post.aecom.yu.edu (post.aecom.yu.edu [129.98.1.100]) 7, 30 -- by draco.aecom.yu.edu (Symantec Mail Security) with ESMTP id 2AAAD718002; 7, 30 -- Wed, 2 Jan 2008 14:48:24 -0500 (EST) 7, 30 -- Received: from AIF3.aecom.yu.edu (aif3.aif.aecom.yu.edu [129.98.80.70]) 7, 30 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 7, 30 -- (No client certificate requested) 7, 30 -- by post.aecom.yu.edu (Postfix) with ESMTP id 20F1C25; 7, 30 -- Wed, 2 Jan 2008 14:48:24 -0500 (EST) 7, 30 -- Message-Id: {7.0.1.0.2.20080102144518.02309670-at-aecom.yu.edu} 7, 30 -- X-Mailer: QUALCOMM Windows Eudora Version 7.0.1.0 7, 30 -- Date: Wed, 02 Jan 2008 14:48:08 -0500 7, 30 -- To: takenomm-at-u.washington.edu, microscopy-at-microscopy.com 7, 30 -- From: Michael Cammer {cammer-at-aecom.yu.edu} 7, 30 -- Subject: Re: [Microscopy] Re: MRI ? on maize embryos 7, 30 -- In-Reply-To: {200712282032.lBSKWJou027643-at-ns.microscopy.com} 7, 30 -- References: {200712282032.lBSKWJou027643-at-ns.microscopy.com} 7, 30 -- Mime-Version: 1.0 7, 30 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 7, 30 -- X-Brightmail-Tracker: AAAAAA== ==============================End of - Headers==============================
Here is the January 2008 Microscopy Today table of contents. I will close the subscription list for this issue on Tuesday January 8, 2008.
Microscopists in North America and MSA members anywhere qualify for free subscriptions. Anyone else may subscribe for US$60 per year (to PARTIALLY cover postage). All subscriptions at http://www.microscopy-today.com .
Thank you, Ron Anderson, Editor ======================== Microscopy Used to Discover New, Cool Mineral! Stephen W. Carmichael1, Mayo Clinic
Material Contrast of Scanning Electron and Ion Microscope Images of Metals T. Suzukia*, M. Kudo*, Y.Sakai*, and T. Ichinokawa**, *JEOL Ltd., Akishima, Tokyo, **Waseda University, Tokyo, Japan
Effective Cell Identification and Segmentation in Fluorescence Microscopy with New Fluorescent Whole Cell Stains Suk J. Hong, Richik N. Ghosh, Thermo Fisher Scientific Inc., Rockford, IL
Giving your SEM or FIB a Helping Hand Neil Rowlands, Oxford Instruments, Concord, USA, Gavin Frayne, Kleindiek Nanotechnik, Tubingen, Germany, Bo Svarrer Hansen, Capres A/S, Lyngby, Denmark
An Introduction to 3D Microscopy Techniques Megan MacNeil and Duncan McMillan, Carl Zeiss MicroImaging, Inc. Thornwood, NY.
Scanning Transmission Electron Microscopy for Critical Dimension Monitoring in Wafer Manufacturing Haifeng Wang*, Jason Fang*, Jason Arjavac**,Rudy Kellner**, *Western Digital Corporation, Fremont, CA, **FEI Company, Hillsboro, OR
Nanoelectromechanics of Inorganic and Biological Systems: From Structural Imaging to Local Functionalities B. J. Rodriguez,1,2 S. V. Kalinin,1,2 S. Jesse,1 G. Thompson,3 A. Vertegel,3 S. Hohlbauch,4 R. Proksch4 ,1Mat. Sci. & Tech, and 2Ctr. for Nanophase Matl. ORNL, Oak Ridge, TN, 3 Clemson University, SC, 4Asylum Research, Santa Barbara, CA
Spatial Resolution in ACOM–What Will Come After EBSD R.A. Schwarzer, Kappstr. 65, D-71083 Herrenberg, Germany
Lab-Tek Chamber Slide for TEM Prep: A Simple, Rapid, and Reliable Protocol for In Situ Embedding Monolayer Cell Cultures in Epoxy and LR White Resin Gang Ning, Penn State University, State College, PA
Imaging Carbon Nanoparticles in Cells Mhairi Gass* & Alexandra Porter**, *SuperSTEM, Daresbury Lab., Daresbury, **Imperial College London, London, U.K.
Automatic Acquisition and Image Analysis of 2D Crystals N. Coudray*, F. Beck**, J.-L. Buessler*, A. Korinek**, A. Karathanou*, H. Rémigy***, H. Kihl*, A. Engel***, J.M. Plitzko**, J.-P. Urban*, *Université de Haute Alsace, Mulhouse, France, **Max Planck Inst. Martinsried, Germany, ***University of Basel, Switzerland
Industry News
NetNotes SAMPLE PREPARATION - osmium ignition SAMPLE PREPARATION – wood for TEM SAMPLE PREPARATION - perchloric acid hazards SAMPLE PREPARATION - differential polymer staining MICROTOMY - alternative ultramicrotome knives IMAGE ANALYSIS - stitching high-resolution microscope images IMMUNOCYTOCHEMISTRY – adherent cells IMMUNOCYTOCHEMISTRY - pre-embedding tissue cultures TEM: Objective aperture vs. EFTEM TEM – alignment problem TEM - lattice fringes TEM - micelle solutions TEM - ice contamination EDX - plants and seeds EDX - biological sample
Dear Abbe
Advertiser's Index
==============================Original Headers============================== 19, 18 -- From randerson20-at-tampabay.rr.com Wed Jan 2 15:01:45 2008 19, 18 -- Received: from hrndva-omtalb.mail.rr.com (hrndva-omtalb.mail.rr.com [71.74.56.122]) 19, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m02L1gmZ031225 19, 18 -- for {Microscopy-at-Microscopy.Com} ; Wed, 2 Jan 2008 15:01:43 -0600 19, 18 -- Received: from [127.0.0.1] (really [24.73.73.214]) 19, 18 -- by hrndva-omta03.mail.rr.com with ESMTP 19, 18 -- id {20080102210140.KPHF2900.hrndva-omta03.mail.rr.com-at-[127.0.0.1]} ; 19, 18 -- Wed, 2 Jan 2008 21:01:40 +0000 19, 18 -- Message-ID: {477BFBB0.5070109-at-tampabay.rr.com} 19, 18 -- Date: Wed, 02 Jan 2008 16:01:36 -0500 19, 18 -- From: Ronald Anderson {randerson20-at-tampabay.rr.com} 19, 18 -- User-Agent: Thunderbird 2.0.0.9 (Windows/20071031) 19, 18 -- MIME-Version: 1.0 19, 18 -- To: Listserver {Microscopy-at-Microscopy.Com} , 19, 18 -- Confocal Microscopy List {CONFOCAL-at-LISTSERV.BUFFALO.EDU} 19, 18 -- Subject: January 2008 Microscopy Today Table of Contents 19, 18 -- Content-Type: text/plain; charset=windows-1252; format=flowed 19, 18 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
Has anyone embedded pine needles in JB-4 and sectioned for LM? I had problem with orientation when I used plastic beem capsules before. I have been trying silicon rubber molds under 10psi. But it doesn't polymerize competely. What is the max psi i can go not to damage both tissue and plastic. I have been also thinking of switching to LR-white. But I dont know if i get better result. I would appreciate any kind of advice on this.
Thanks.
Sau Silwal
Send instant messages to your online friends http://uk.messenger.yahoo.com
==============================Original Headers============================== 7, 19 -- From sau_silwal-at-yahoo.com Wed Jan 2 15:16:11 2008 7, 19 -- Received: from web30112.mail.mud.yahoo.com (web30112.mail.mud.yahoo.com [209.191.69.44]) 7, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m02LGB8F011221 7, 19 -- for {Microscopy-at-Microscopy.Com} ; Wed, 2 Jan 2008 15:16:11 -0600 7, 19 -- Received: (qmail 58393 invoked by uid 60001); 2 Jan 2008 21:16:10 -0000 7, 19 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 7, 19 -- s=s1024; d=yahoo.com; 7, 19 -- h=X-YMail-OSG:Received:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 7, 19 -- b=OtgCzeGTpLV1pbFgc3Y11EJS0De4rrMzsoukd0VL3Ba+tqHRFzXqiEDkddOqKTuX3DlYbx5KhHtN+j8mT4QEArNfI5neC1lVxErBFcPaO5ujyYp32hxRMMVUQhO6a0dtwyez40q6rqVqolb5jRrBH0o5GJcj44y78ZnXcgMqhHw=; 7, 19 -- X-YMail-OSG: k1zyU3wVM1le9chAoqIW7gcRZBdH5fik_w0y5g286myMXe9z5iWTOf9UY07vpCxeUCJJja.zbDZT4_naZRIDdU4ltAwA1J4fXq8lzsq4W.pnp55Gt2jxxIiJOGXuyg-- 7, 19 -- Received: from [65.188.180.191] by web30112.mail.mud.yahoo.com via HTTP; Wed, 02 Jan 2008 21:16:10 GMT 7, 19 -- Date: Wed, 2 Jan 2008 21:16:10 +0000 (GMT) 7, 19 -- From: Saubhagya Silwal {sau_silwal-at-yahoo.com} 7, 19 -- Subject: Re: Embedding pine needles in JB-4 7, 19 -- To: Microscopy-at-Microscopy.Com 7, 19 -- MIME-Version: 1.0 7, 19 -- Content-Type: text/plain; charset=iso-8859-1 7, 19 -- Content-Transfer-Encoding: 8bit 7, 19 -- Message-ID: {757701.58226.qm-at-web30112.mail.mud.yahoo.com} ==============================End of - Headers==============================
Has anyone embedded pine needles in JB-4 and sectioned for LM? I had problem with orientation when I used plastic beem capsules before. I have been trying silicon rubber molds under 10psi. But it doesn't polymerize competely. What is the max psi i can go not to damage both tissue and plastic. I have been also thinking of switching to LR-white. But I dont know if i get better result. I would appreciate any kind of advice on this.
Thanks.
Sau Silwal
Send instant messages to your online friends http://uk.messenger.yahoo.com
==============================Original Headers============================== 7, 19 -- From sau_silwal-at-yahoo.com Wed Jan 2 15:16:51 2008 7, 19 -- Received: from web30104.mail.mud.yahoo.com (web30104.mail.mud.yahoo.com [209.191.69.36]) 7, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m02LGmYY012542 7, 19 -- for {Microscopy-at-Microscopy.Com} ; Wed, 2 Jan 2008 15:16:51 -0600 7, 19 -- Received: (qmail 90828 invoked by uid 60001); 2 Jan 2008 21:16:48 -0000 7, 19 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 7, 19 -- s=s1024; d=yahoo.com; 7, 19 -- h=X-YMail-OSG:Received:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 7, 19 -- b=T68d+yta5cWOzfibtEsYoAkJNlZ8jr7ckxVfvtrsAyzuJCy2WtA2Xgw5px2NrMBAAEK/Q8UaE9taqy8zSNuw+t3x3102mEgxwvkMB15fuRvMKquO+iL/A+HkYKH7zGdKTEjwWGm5iJhiu8NiF+8vOVtF/MgbyBWSFK/AoOao9WE=; 7, 19 -- X-YMail-OSG: CG53fXUVM1nltvLPOODYEEh5AffxIXkERFL5lqXHzwzlPPLehI7pK7gYOYMEYo2ytw-- 7, 19 -- Received: from [65.188.180.191] by web30104.mail.mud.yahoo.com via HTTP; Wed, 02 Jan 2008 21:16:46 GMT 7, 19 -- Date: Wed, 2 Jan 2008 21:16:46 +0000 (GMT) 7, 19 -- From: Saubhagya Silwal {sau_silwal-at-yahoo.com} 7, 19 -- Subject: Re: Embedding pine needles in JB-4 7, 19 -- To: Microscopy-at-Microscopy.Com 7, 19 -- MIME-Version: 1.0 7, 19 -- Content-Type: text/plain; charset=iso-8859-1 7, 19 -- Content-Transfer-Encoding: 8bit 7, 19 -- Message-ID: {902929.90487.qm-at-web30104.mail.mud.yahoo.com} ==============================End of - Headers==============================
Sau, Not sure exactly if this will solve your problem, but for what its worth, you can get capsules that are like BEEMs except that they have a completely flat bottom. They are made by TAAB but available from major supply houses (no finanical connection). This gives a nice 7 mm (or so) flat surface. You can also get this out of a standard BEEM by flipping it upside down, although you have to mess about to keep the resin from leaking (definitely a mess, but possible to do).
Hope this helps, Tobias
} } Hi, } } Happy New Year!! } } Has anyone embedded pine needles in JB-4 and sectioned } for LM? I had problem with orientation when I used } plastic beem capsules before. I have been trying } silicon rubber molds under 10psi. But it doesn't } polymerize competely. What is the max psi i can go not } to damage both tissue and plastic. } I have been also thinking of switching to LR-white. } But I dont know if i get better result. I would } appreciate any kind of advice on this. } } Thanks. } } Sau Silwal }
Sorry for the late reply, but you might contact Jamie Weichert at UW-Madison: http://www.radiology.wisc.edu/research/contrastAgentLab/index.php
He works on this sort of problem for both MRI and microCT. The issue is more likely, can the instruments get the resolution you need? But I suspect they can.
Phil
} } Daar coleagues } I am working on a project on the vascular puncture inoculation } (VPI) of maize viruses into maize embyos. We need to have a 3-D } picture of the vascular bundles in (germinating) maize embyos, and a } colleage of mine suggested MRI as a possible means to achieve that. } I wonder if anyone has tried MRI on plants/ plant tissues, or if } someone can suggest another more suitable method (apart from } LM-serial sectioning). Thanks and Happy New Year to everyone. } } } El-Desouky Ammar, Ph.D. } Dept. of Entomology, OSU, } 019 Selby Hall, } OARDC, Wooster, OH 44691 } Tel: 330-263-3830. } FAX: 330-202-3563. -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 4, 22 -- From oshel1pe-at-cmich.edu Thu Jan 3 09:00:55 2008 4, 22 -- Received: from ob2.cmich.edu (ob2.cmich.edu [141.209.20.21]) 4, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m03F0tDU006319 4, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 3 Jan 2008 09:00:55 -0600 4, 22 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 4, 22 -- by ob2.cmich.edu (8.12.10/8.12.10) with ESMTP id m03FIA9N005799 4, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 3 Jan 2008 10:18:10 -0500 4, 22 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.1830); 4, 22 -- Thu, 3 Jan 2008 10:00:05 -0500 4, 22 -- Mime-Version: 1.0 4, 22 -- Message-Id: {f06240808c3a2a83b8dca-at-[141.209.160.249]} 4, 22 -- In-Reply-To: {200712281726.lBSHQR4i001693-at-ns.microscopy.com} 4, 22 -- References: {200712281726.lBSHQR4i001693-at-ns.microscopy.com} 4, 22 -- Date: Thu, 3 Jan 2008 10:00:48 -0500 4, 22 -- To: Microscopy-at-microscopy.com 4, 22 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 4, 22 -- Subject: Re: [Microscopy] MRI ? on maize embryos 4, 22 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 4, 22 -- X-OriginalArrivalTime: 03 Jan 2008 15:00:05.0856 (UTC) FILETIME=[5349E600:01C84E19] 4, 22 -- X-CanItPRO-Stream: default 4, 22 -- X-Spam-Score: -4 () L_EXCH_MF 4, 22 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.21 ==============================End of - Headers==============================
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I am an amateur naturalist. I have made by myself a microtome and I also use to honing the blade with results quite good. Now I am testing a disposable blade. It was a kindly gift of a friend of mine who works in an histology laboratory. I wonder where could I find disposable blades because it seems they are working well . Otherwise it is quite difficult to make and honing a blade by myself. Does anybody know a factory that produces such kind of blades? Any suggestions would be greatly appreciated.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.org/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both Jill.Verlander-at-medicine.ufl.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
We returned to work today after a hiatus since 12/21. The afternoon before we shut down the lab for Christmas break, we turned off the scopes and chillers. All was well with the equipment before that.
Today, we tried to get everything going again, but the old Coolwell chiller (model S-075W) on our Zeiss EM10 is not working properly. When we tried to run it, there was no flow and the HiTemp warning light on the front panel was lit. This is supposed to shut off the system if the temp rises above 93 degrees F, which it is not (room temperature is 73 degrees, coolant temp about 71 degrees F). However, the "reset" switch on the P6 high temperature safety control box had not popped out.
If you turn the unit on and press the button (S-3) that bridges the high temperature limit control, and have the unit pumping only on itself (a short loop from supply to return), the pump runs normally. Intermittently, it will run normally either on "bypass" (which does not allow the compressor to come on) or on "normal" function. The compressor will come on when the unit is working and set on "normal." However, the unit will spontaneously shut down, and when it does, the hi temperature light comes on.
The compressor is cooled by house tap water: the supply and return lines are open and flowing. The coolant tank is full, magnetic float switch floating, and the filter looks okay - not pristine, but only slightly gray. We did have a "heat event" according to an unrelated computer that was left on during the break, but I don't see how that could have affected the chiller, since it was shut off at the time.
Does anybody have any suggestions as to what might be causing this problem and how to correct it?
Many thanks, and Happy New Year to all!
Jill Verlander Reed University of Florida College of Medicine Electron Microscopy Core Facility
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Email: jeff-at-tss-consulting.com Name: Jeff West
Organization: TSS Consulting Ltd.
Title-Subject: [Filtered] Employment Opportunity
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A minimum of 3 years experience with TEM sample prep and imaging is required. Preference is for candidates with Materials Science or Engineering degrees (BS or MS).
For further information and to apply for this position please contact: Jeff West TSS Consulting, Ltd. (877) 489-2425 Resumes may be sent to: jeff-at-tss-consulting.com
This Question was submitted to Ask-A-Microscopist by (susan.trant-at-viha.ca) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Monday, December 31, 2007 at 15:28:34 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both susan.trant-at-viha.ca as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Email: susan.trant-at-viha.ca Name: Susan Trant
Education: Graduate College
Location: Victoria, British Columbia, Canada
Title: EM film
Question: Hello everyone
I purchased Eastman Fine Grain Release Positive Film 5302 from Electron Microscopy Sciences for my TEM. The film is much thicker than the current product that I am using. I contacted some photo experts and they said that I needed a product called Dektol for a film developer.(Kodak) Does anyone use this film and if so, what developer do you use and what temperature and length of time for processing? The photography shop that I talked to also said that you do not dilute the stock solution before use. We are receiving a new digital imaging system within the year.
Thanks
Sue Trant EM Technologist Vancouver Island Health Authority Victoria, British Columbia, Canada
I trashed my files on this film about six months ago thinking that I'd never need to consult it again. There are several things that I do remember that may be of interest to you. The following is from a 25 year old memory!
This film is quite good for TEM and the sheet film is thick also. You will need to do an exposure test to see what gives the best results. If you have an older scope you should be able to change the exposure time. Do several times of the same area.
Keep film away from light! I used a very dark red filtered light that was so dark that it was nearly useless even after giving my eyes time to adjust. To load the film both into the scope camera holder and into the developing tank I would turn my back to the light and load in front of me.
Dektol seems correct as the developer. I do not remember diluting it - try using it straight from the stock solution that you make from the powder. Adjust the temperature before turning out the room light. Try hot water around a beaker with the developer in it and stir gently with the thermometer. I used a brown gal. bottle to store the developer, kept in the darkroom for a month or so with no problems as to age. Just make sure the bottle is stoppered well. If the developer starts to turn brown it is time to make up a new batch. I think the temperature for making the developer is much hotter than the film developing temperature so make sure you make it up with enough time to cool before you want to use it. (Stoppers work better than caps in my opinion)
Four minute development stands out in my mind; agitated several times. I used the old apron type from Kodak to do the loading but any standard 35 mm developing tank should work well. Make positive that you do not have any bubbles stuck to the film by banging the tank on a counter surface a few times after the developer is in the tank with the film. Water rinse twice. Kodak Rapid fixer 4 min. with agitation several times Wash in running for 30 min. Photoflo rinse for 15 sec. Hang to dry with a weight on the bottom (to eliminate curling)
I used the film without perforations (holes along the sides). This increased the area of the image greatly. If you do have a camera that can use this film and have a negative holder that you can spare, find a shop that can make the viewing holes of the holder larger by a few mm. If scanning into a computer there will be no problem.
Hoping my recollections are accurate, Pat
Patricia Stranen Connelly NHLBI Electron Microscopy Core National Institutes of Health 14 Service Road South Bldg. 14E Rm. 111B MSC 5570 Bethesda, MD 20892-5570 Phone 301-496-3491 connellyps-at-mail.nih.gov
} ---------------------------------------------------------------------------- } Email: susan.trant-at-viha.ca } Name: Susan Trant } } Education: Graduate College } Location: Victoria, British Columbia, Canada } Title: EM film } } I purchased Eastman Fine Grain Release Positive Film 5302 from Electron } Microscopy Sciences for my TEM. The film is much thicker than the current } product that I am using. I contacted some photo experts and they said that I } needed a product called Dektol for a film developer.(Kodak) Does anyone use } this film and if so, what developer do you use and what temperature and length } of time for processing? The photography shop that I talked to also said that } you do not dilute the stock solution before use. } We are receiving a new digital imaging system within the year. } } Thanks } } Sue Trant } EM Technologist } Vancouver Island Health Authority } Victoria, British Columbia, } Canada
} ==============================End of - Headers==============================
==============================Original Headers============================== 14, 24 -- From connellyps-at-nhlbi.nih.gov Thu Jan 3 10:41:04 2008 14, 24 -- Received: from NIHCESSMTP2.hub.nih.gov (nihcessmtp2.hub.nih.gov [128.231.90.116]) 14, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m03Gf4Bh003100 14, 24 -- for {microscopy-at-microscopy.com} ; Thu, 3 Jan 2008 10:41:04 -0600 14, 24 -- Received: from NIHCESMLBX6.nih.gov ([156.40.71.206]) by NIHCESSMTP2.hub.nih.gov with Microsoft SMTPSVC(6.0.3790.1830); 14, 24 -- Thu, 3 Jan 2008 11:40:55 -0500 14, 24 -- Received: from 156.40.71.188 ([156.40.71.188]) by NIHCESMLBX6.nih.gov ([156.40.71.206]) via Exchange Front-End Server mail.nih.gov ([156.40.71.167]) with Microsoft Exchange Server HTTP-DAV ; 14, 24 -- Thu, 3 Jan 2008 16:40:54 +0000 14, 24 -- User-Agent: Microsoft-Entourage/11.3.6.070618 14, 24 -- Date: Thu, 03 Jan 2008 11:38:30 -0500 14, 24 -- Subject: Re: [Microscopy] AskAMicroscopist: EM film 5302 14, 24 -- From: Patricia Connelly {connellyps-at-nhlbi.nih.gov} 14, 24 -- To: {susan.trant-at-viha.ca} 14, 24 -- CC: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 14, 24 -- Message-ID: {C3A279B6.12C8%connellyps-at-nhlbi.nih.gov} 14, 24 -- Thread-Topic: [Microscopy] AskAMicroscopist: EM film 5302 14, 24 -- Thread-Index: AchOJxJuUQ2CdroaEdygxgANk2Yv1A== 14, 24 -- In-Reply-To: {200801031512.m03FCW6H008302-at-ns.microscopy.com} 14, 24 -- Mime-version: 1.0 14, 24 -- Content-type: text/plain; 14, 24 -- charset="ISO-8859-1" 14, 24 -- X-OriginalArrivalTime: 03 Jan 2008 16:40:55.0489 (UTC) FILETIME=[6926A310:01C84E27] 14, 24 -- Content-Transfer-Encoding: 8bit 14, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m03Gf4Bh003100 ==============================End of - Headers==============================
You are invited to attend a live, interactive, web-based instructional seminar:
========================================================== "Quantitative Image and Data Acquisition for Fluorescent Specimens" Advice from a Facility Director
Presented by Brian Matsumoto, Ph.D., University of California, Santa Barbara ==========================================================
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When: ===================== Monday, 7-January, 1:30PM (New York time; 10:30 AM California time.) Duration: Approximately 1 hour.
Attendees will learn about issues affecting the quantitative accuracy of fluorescence images acquired through a microscope and will pick up tips and suggestions for improving image quality, making better use of the camera's light collection abilities, and will learn the nomenclature associated with digital imaging. Attendees will leave with a better understanding of how to characterize and optimize their optical system, camera, and software. Bring your questions to this live, interactive web-based seminar.
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About the presenter ===================== Brian Matsumoto, Ph.D. is the Director of the Integrated Microscopy Facility and is an Associate Adjunct Professor for the department of Molecular, Cellular and Developmental Biology Department at the University of California Santa Barbara. He is the author of "Basic Methods in Light Microscopy" (Cambridge University Press) and editor of "Cell Biological Applications of Confocal Microscopy" (Academic Press).
More instructional webinars are also shown at http://magbiosystems.com/education Register for any session of interest and feel encouraged to pass this along to your colleagues.
Sponsored by the Microimaging Applications Group (MAG), a group of independent imaging companies working cooperatively to provide an unparalleled range of solutions for microimaging applications.
This seminar requires that attendees use a Java-enabled browser with a high bandwidth connection. Audio is via toll-free telephone.
There is no charge to participate in this or the other on-line seminars.
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Email: samuel.connell-at-stjude.org Name: Samuel Connell
Organization: St. Jude Childrenís Research Hospital
Title-Subject: [Filtered] Imaging Scientist ñ St. Jude Childrenís Research Hospital
Question: Imaging Scientist ñ St. Jude Childrenís Research Hospital.
Currently, St. Jude Childrenís Research Hospital has an opening for an Imaging Scientist (Job Number 17216) in the Cellular Imaging Department.
The successful candidate would be responsible for assisting faculty, postdoctoral researchers, staff, and collaborators in advance microscopy techniques and methodologies, including laser scanning and spinning disk confocal fluorescence microscopy, wide-field microscopy, live cell imaging, FRET, FLIM, FRAP and TIRF, as well as routine maintenance of the instrumentation infrastructure.
Requirements:
A Bachelor's degree in an appropriate scientific field plus a minimum of ten (10) years (post-degree) of relevant and productive work experience is required OR,
A Master's degree in an appropriate scientific field plus a minimum of nine (9) years (post-degree) of relevant and productive work experience is required OR,
A PhD in an appropriate scientific field plus five (5) years (post-degree) of relevant and productive work experience is required.
To apply please visit our Web site www.stjude.org/jobs
St. Jude Childrenís Research Hospital is an Equal Opportunity Employer.
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Email: mlibbee-at-gmail.com Name: Marissa
Title-Subject: [Filtered] Denton Desk II Maintenance
Question: Greetings! I want to clean up the Denton Desk II sputter coater (equipped with AuPd target) in my lab facility. Does anyone have any suggestions as to how I to clean the plastic encasing? Are there any solvents safe to use for this purpose?
If you are active in or interested in microscopy education, and would like details about an M&M 08 symposium on teaching microscopy and microanalysis, please reply offline by sending your contact information to:
eschumacher-at-mccrone.com
We would like to compile an email address list so that we can send information about the symposium directly to those who would like to receive it. The symposium will cover programs for classroom teaching at all levels, and other methods of training. Please feel free to forward this request to interested colleagues.
Thank you,
Elaine Schumacher, McCrone Associates Charles Lyman, Lehigh University
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Based on your words "disposable" and 'blade', I think you should try disposable Weck Prep Extra Long Blades (2.25 inches long). These are available from various EM supply houses in the US and maybe elsewhere. They do say Weck on them. So check and see if that name is on one side of the blade you got from histology. Some biologists call them surgical blades. So check with your friend to see if that is what he gave you.
In any case, these are single edged "razor blades" designed for surgical cutting or chopping of tissue. Weck Blades are much sharper than regular "razor blades". In my opinion, they are about 3-5 times sharper than ordinary razor blades based on my experience using them to block trim hard Epon epoxy.
Once you start using them, you will stop using regular razor blades and ignore the extra cost.
HTH,
Paul.
At 09:03 AM 1/3/08 -0600, you wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
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Plastic encasing? Are you sure? Our Denton Desk II has a glass cylinder, which we regualrly clean (the inside) of simply but carefully running a new (cleaned) razor blade around. Slides on the glass and peels of the metal. We remove the L-seals, and then we use an ethanol soaked cotton coth to remove any remaining bits and finger Prints. The aluminium bits we polish with a "Green-Scratchey-thing" (3M) and more ethanol, then wiped down with toweling.
The only "Plastic" is the teflon bits inside, and again we use ethanol and paper towels or cotton cloth to polish them up. The case work is painted metals and we use general purpose glass/surface cleaner on it.
On 3 Jan 2008 at 14:58, mlibbee-at-gmail.com wrote:
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Richard E. Edelmann, Ph.D. Electron Microscopy Facility Director 364 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.emf.muohio.edu
==============================Original Headers============================== 10, 25 -- From edelmare-at-muohio.edu Fri Jan 4 11:57:15 2008 10, 25 -- Received: from mulnx12.mcs.muohio.edu (mulnx12.mcs.muohio.edu [134.53.6.67]) 10, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m04HvF9R021636 10, 25 -- for {microscopy-at-Microscopy.com} ; Fri, 4 Jan 2008 11:57:15 -0600 10, 25 -- Received: from mulnx23.mcs.muohio.edu (mulnx23.mcs.muohio.edu [134.53.6.10]) 10, 25 -- by mulnx12.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id m04HulEV020683; 10, 25 -- Fri, 4 Jan 2008 12:56:47 -0500 10, 25 -- Received: from [192.168.1.23] ([134.53.14.105]) 10, 25 -- by mulnx23.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id m04Hukvd000531; 10, 25 -- Fri, 4 Jan 2008 12:56:46 -0500 10, 25 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} 10, 25 -- To: "mlibbee-at-gmail.com" {mlibbee-at-gmail.com} 10, 25 -- Date: Fri, 04 Jan 2008 12:56:46 -0500 10, 25 -- MIME-Version: 1.0 10, 25 -- Subject: Re: [Microscopy] viaWWW: Denton Desk II Maintenance 10, 25 -- CC: microscopy-at-Microscopy.com 10, 25 -- Message-ID: {477E2D0E.8384.5D96491-at-edelmare.muohio.edu} 10, 25 -- Priority: normal 10, 25 -- In-reply-to: {200801031958.m03JwshZ003654-at-ns.microscopy.com} 10, 25 -- References: {200801031958.m03JwshZ003654-at-ns.microscopy.com} 10, 25 -- X-mailer: Pegasus Mail for Windows (4.41) 10, 25 -- Content-type: text/plain; charset=US-ASCII 10, 25 -- Content-transfer-encoding: 7BIT 10, 25 -- Content-description: Mail message body 10, 25 -- X-Scanned-By: MIMEDefang 2.57 on 134.53.6.67 ==============================End of - Headers==============================
I have a full set of Kidak instructions for 5302. I can scan them and send along if you would like.
Oh, here's Kodak's online:
http://www.kodak.com/US/plugins/acrobat/en/motion/products/lab/h15302. pdf
But to answer:
Safelight: Kodak OA (greenish Yellow) or 1A (the deep dark red mentioned by Patricia Stranen Connelly).
develop:
20C
Dektol (1part dektol : 2 parts water) 2 to 4 mins.
Rinse (water or stopbath)
Fix Kodak fixer 2 to 4 mins
Wash 15 to 20 mins in water or (Hypoclear 1-2 mins then 5 min wash).
I used to regularly use this film for making black and white slides by contact printing B&W Negs. I have never used it for direct EM Exposure. But as far as I know it is NOT a directly invertable film (i.e. it will not give you a positive image after exposing it directly to the beam.) It is not a "35mm slide" type of film (without some fancy developing techniques).
On 3 Jan 2008 at 10:06, susan.trant-at-viha.ca wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question was submitted to Ask-A-Microscopist by (susan.trant-at-viha.ca) } from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Monday, December 31, 2007 at 15:28:34 } Remember to consider the Grade/Age of the student when considering the Question } --------------------------------------------------------------------------- } Please reply to both susan.trant-at-viha.ca as well as to the Microscopy Listserver } --------------------------------------------------------------------------- } } Email: susan.trant-at-viha.ca } Name: Susan Trant } } Education: Graduate College } } Location: Victoria, British Columbia, Canada } } Title: EM film } } Question: Hello everyone } } I purchased Eastman Fine Grain Release Positive Film 5302 from } Electron Microscopy Sciences for my TEM. The film is much thicker } than the current product that I am using. I contacted some photo } experts and they said that I needed a product called Dektol for a film } developer.(Kodak) Does anyone use this film and if so, what developer } do you use and what temperature and length of time for processing? The } photography shop that I talked to also said that you do not dilute the } stock solution before use. We are receiving a new digital imaging } system within the year. } } Thanks } } Sue Trant } EM Technologist } Vancouver Island Health Authority } Victoria, British Columbia, } Canada } } } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 10, 11 -- From zaluzec-at-ultra5.microscopy.com Thu Jan 3 09:04:46 2008 } 10, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 10, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m03F4iEP015281 } 10, 11 -- for {microscopy-at-microscopy.com} ; Thu, 3 Jan 2008 09:04:45 -0600 } 10, 11 -- Mime-Version: 1.0 } 10, 11 -- Message-Id: {p06240806c3a2a9f2f357-at-[206.69.208.22]} } 10, 11 -- Date: Thu, 3 Jan 2008 09:04:43 -0600 } 10, 11 -- To: microscopy-at-microscopy.com } 10, 11 -- From: susan.trant-at-viha.ca (by way of Ask-A-Microscopist) } 10, 11 -- Subject: AskAMicroscopist: EM film 5302 } 10, 11 -- Content-Type: text/plain; charset="us-ascii" } ==============================End of - Headers==============================
Richard E. Edelmann, Ph.D. Electron Microscopy Facility Director 364 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.emf.muohio.edu
==============================Original Headers============================== 20, 25 -- From edelmare-at-muohio.edu Fri Jan 4 13:39:02 2008 20, 25 -- Received: from mulnx12.mcs.muohio.edu (mulnx12.mcs.muohio.edu [134.53.6.67]) 20, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m04Jd1PT004770 20, 25 -- for {microscopy-at-Microscopy.com} ; Fri, 4 Jan 2008 13:39:02 -0600 20, 25 -- Received: from mulnx23.mcs.muohio.edu (mulnx23.mcs.muohio.edu [134.53.6.10]) 20, 25 -- by mulnx12.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id m04Jd1aM002755; 20, 25 -- Fri, 4 Jan 2008 14:39:01 -0500 20, 25 -- Received: from [192.168.1.23] ([134.53.14.105]) 20, 25 -- by mulnx23.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id m04Jd1rv024391; 20, 25 -- Fri, 4 Jan 2008 14:39:01 -0500 20, 25 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} 20, 25 -- To: "susan.trant-at-viha.ca" {susan.trant-at-viha.ca} 20, 25 -- Date: Fri, 04 Jan 2008 14:39:00 -0500 20, 25 -- MIME-Version: 1.0 20, 25 -- Subject: Re: [Microscopy] AskAMicroscopist: EM film 5302 20, 25 -- CC: microscopy-at-Microscopy.com 20, 25 -- Message-ID: {477E4504.31396.636FA6B-at-edelmare.muohio.edu} 20, 25 -- Priority: normal 20, 25 -- In-reply-to: {200801031506.m03F6DV2020709-at-ns.microscopy.com} 20, 25 -- References: {200801031506.m03F6DV2020709-at-ns.microscopy.com} 20, 25 -- X-mailer: Pegasus Mail for Windows (4.41) 20, 25 -- Content-type: text/plain; charset=US-ASCII 20, 25 -- Content-transfer-encoding: 7BIT 20, 25 -- Content-description: Mail message body 20, 25 -- X-Scanned-By: MIMEDefang 2.57 on 134.53.6.67 ==============================End of - Headers==============================
Ok. Before you say that I'm glutton for punishment, just remember that I really love playing with these kinds of toys...
I just picked up an ISI Mini-SEM, and got it home to play with. It looked simple enough that I could start breaking apart the systems of the SEM and analyzing them independently. I've got the main blue control box with two modules in it, a brown box with what I assume is the HV system, the chamber and column assembly (This is the smallest chamber I've ever seen!), and the tan voltage converter box. Is there one more piece somewhere that I should have? Does anybody have any documentation on one of these that they could copy a page or two for me?
Thanks,
Justin A. Kraft
==============================Original Headers============================== 4, 27 -- From kraftpiano-at-gmail.com Fri Jan 4 14:49:58 2008 4, 27 -- Received: from rv-out-0910.google.com (rv-out-0910.google.com [209.85.198.191]) 4, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m04KnvXY018803 4, 27 -- for {microscopy-at-microscopy.com} ; Fri, 4 Jan 2008 14:49:58 -0600 4, 27 -- Received: by rv-out-0910.google.com with SMTP id k20so5235092rvb.30 4, 27 -- for {microscopy-at-microscopy.com} ; Fri, 04 Jan 2008 12:49:57 -0800 (PST) 4, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 27 -- d=gmail.com; s=gamma; 4, 27 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 4, 27 -- bh=UoJrkFgZvjOmsXeWVmx7z0cgeySQ64eop4mrdlZvpbI=; 4, 27 -- b=x1Xubwjgf4r/aDTxjY0beo3jKuxwTbC7F4LOKDw+MyBu7eTCSP93MNSzoUVTmR/Mjpc2rAzsZ4FLc5N/wlkRp9IvRkOnW03VMYHAj5G/yzOuPnR6JpHeaqs5321Pbnc5tMLbBsr9mcmf7+OyhOgR/uY1N+3UbWFnoU92CazLnk4= 4, 27 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 4, 27 -- d=gmail.com; s=gamma; 4, 27 -- h=message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 4, 27 -- b=PMpGz08Zn0JxS3va82Mur3CpqCUc8dMK3GyuOnVTpy8l4+bh89hPwVM3VsQYavQKv+pTLbzIfQApuJASu2RLAnVWPx/RBA2Gdy6ETzvnbpgiJYfamOOljURMw5sA6mZeZ8/QTRRwrsBqWQWJEw5pZukuv8gr8K7hl+Wntt6WaqM= 4, 27 -- Received: by 10.141.172.6 with SMTP id z6mr4982758rvo.136.1199479797076; 4, 27 -- Fri, 04 Jan 2008 12:49:57 -0800 (PST) 4, 27 -- Received: by 10.140.161.11 with HTTP; Fri, 4 Jan 2008 12:49:57 -0800 (PST) 4, 27 -- Message-ID: {25e2b0d20801041249o6e2ab2fft231e300d7cb48422-at-mail.gmail.com} 4, 27 -- Date: Fri, 4 Jan 2008 15:49:57 -0500 4, 27 -- From: "Justin Kraft" {kraftpiano-at-gmail.com} 4, 27 -- To: microscopy-at-microscopy.com 4, 27 -- Subject: ISI Mini-SEM 4, 27 -- MIME-Version: 1.0 4, 27 -- Content-Type: text/plain; charset=ISO-8859-1 4, 27 -- Content-Transfer-Encoding: 7bit 4, 27 -- Content-Disposition: inline ==============================End of - Headers==============================
Unlike Pat, I'm still using 5302. And unlike edelmare (sorry, seen your postings but we've never met and I don't know your name) I have always used it for EM. Did try to use it as fine grain film for pictures of gels many years ago, but when a 1 min exposure at F1.8 did not give sufficient exposure I gave that up. First, you are right, the base is more thick than the estar bases you are familiar with. I assume you have the sprocket film - Kodak stopped producing the sproketless some years ago. The problem with the sprocketless film was that apparently during the cutting process the film was friction fed. As a result you got an occasional scratch due to a piece of dust getting in the system between the gel and a roller. Scratches tended to be quite long, and gave the idea of 35mm film a bad name. There are essentially no problems with scratches on the sprocket film. The thicker base makes it also work better when loading and putting into developer spools - there is less chance of breaking the film at the sprocket hole. This means the Paterson steel spools work well.
As Pat said, the green/yellow OA filter is suffcient. Leaves plenty of light to work with in the dark room.
As far as developer - we have alwoays used the D-19 developer, full strength. Get packages to make 1G US and put into a collapsable bag - say the type juice concentrates come in for wine making kits. This reduces air head room and prevents oxidation of the developer. It will keep a lot longer that way. I'm not sure about the current status for getting developer from Kodak Canada. The last order we placed took some time. I have the recipe for making it from scratch and can send it to you next week if you need. On break for the rest of the week and not back in the lab until Monday.
I under-expose slightly and push develop. The time is 3:30 at 20-22C. If compulsive you wash with a week acetic acid stop solution (1% of stock, or about 0.4% true concentration acetic acid). Then into any clearing bath for 5 minutes.
The film has really fine grain. I regularly enlarge 10x for working prints and make publication prints at 4-5 times enlargement. You will be quite happy with the results once you get the exposure times worked out.
Since you are doing film, what chemistry are you using for your printing?
Also, Tina - if you see this - sorry about the Sugar Bowl, but if I'b been in New Orleans, the Rainbow Warriors would have been worth the price of admission alone.
And regards the World Under 20's in Pardubice - for the rest of you guys on the wrong side of the 49th - final score Canada 4, US 1
Go Canada Go.
Paul
Paul R. Hazelton, PhD Electron Microscope Unit University of Manitoba Department of Medical Microbiology 531 Basic Medical Sciences Building 730 William Avenue Winnipeg, Manitoba, Canada, R3E 0W3 e-mail: paul_hazelton-at-umanitoba.ca Phone:204-789-3313 Cell:204-781-6982 Fax:204-789- 3926
==============================Original Headers============================== 13, 22 -- From paul_hazelton-at-umanitoba.ca Fri Jan 4 15:32:53 2008 13, 22 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.23]) 13, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m04LWr6O032295 13, 22 -- for {microscopy-at-microscopy.com} ; Fri, 4 Jan 2008 15:32:53 -0600 13, 22 -- Received: from [192.168.100.101] (wnpgmb01dc2-104-85.dynamic.mts.net [142.161.104.85]) 13, 22 -- (authenticated bits=0) 13, 22 -- by electra.cc.umanitoba.ca (8.14.2/8.14.2) with ESMTP id m04LWm3b015751 13, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO); 13, 22 -- Fri, 4 Jan 2008 15:32:50 -0600 (CST) 13, 22 -- Message-ID: {477EA600.90903-at-umanitoba.ca} 13, 22 -- Date: Fri, 04 Jan 2008 15:32:48 -0600 13, 22 -- From: Paul Hazelton {paul_hazelton-at-umanitoba.ca} 13, 22 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.7.2) Gecko/20040804 Netscape/7.2 (ax) 13, 22 -- X-Accept-Language: en-us, en 13, 22 -- MIME-Version: 1.0 13, 22 -- To: susan.trant-at-viha.ca, Microscopy Listserver {microscopy-at-microscopy.com} , 13, 22 -- Philip Oshel {oshel1pe-at-cmich.edu} 13, 22 -- Subject: Re: [Microscopy] AskAMicroscopist: EM film 5302 13, 22 -- References: {200801031507.m03F7i5T026197-at-ns.microscopy.com} 13, 22 -- In-Reply-To: {200801031507.m03F7i5T026197-at-ns.microscopy.com} 13, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 13, 22 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
There will be a PreMeeting Workshop covering ESEM at the ACMM-20 Meeting in Perth Western Australia in Feb. 2008.
Here is the meeting URL : http://www.microscopy.org.au/ACMM20
Follow the link to Workshops.
Nestor Your Friendly Neighborhood SysOp
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 11, 14 -- From zaluzec-at-microscopy.com Sat Jan 5 09:48:15 2008 11, 14 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 11, 14 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m05FmEKJ027791; 11, 14 -- Sat, 5 Jan 2008 09:48:14 -0600 11, 14 -- Mime-Version: 1.0 11, 14 -- Message-Id: {p06240800c3a55694015d-at-[206.69.208.22]} 11, 14 -- In-Reply-To: {200801050918.m059IOem029941-at-ns.microscopy.com} 11, 14 -- References: {200801050918.m059IOem029941-at-ns.microscopy.com} 11, 14 -- Date: Sat, 5 Jan 2008 09:48:13 -0600 11, 14 -- To: microscopy-at-microscopy.com 11, 14 -- From: "Nestor J. Zaluzec" {zaluzec-at-microscopy.com} 11, 14 -- Subject: Re: Training Course on ESEM 11, 14 -- Cc: mmiralles-at-pi.ac.ae 11, 14 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers==============================
1.) I would definitely switch to LR white. We never use methacrylate resins anymore.
2.) The flat-bottomed capsules recommended by Tobias are much better for polymerizing LR white than BEEM-type capsules. The walls seem to be thicker and are therefore less permeable to oxygen. Also, they are offered in polypropylene. The PP capsules are really a pain to get the blocks out of, but they polymerize really nicely.
3.) I was told that putting LR white (and maybe methacrylate?) resins under vacuum can cause the initiator to evaporate from the resin, causing incomplete polymerization. If you need to vacuum to remove air from your needles, I would do it during fixation or during the post-fixation wash. If you want further details, just email me.
Have fun,
Andy Bowling
-----Original Message----- X-from: baskin-at-bio.umass.edu [mailto:baskin-at-bio.umass.edu] Sent: Wednesday, January 02, 2008 3:50 PM To: Bowling, Andrew
Sau, Not sure exactly if this will solve your problem, but for what its worth, you can get capsules that are like BEEMs except that they have a completely flat bottom. They are made by TAAB but available from major supply houses (no finanical connection). This gives a nice 7 mm (or so) flat surface. You can also get this out of a standard BEEM by flipping it upside down, although you have to mess about to keep the resin from leaking (definitely a mess, but possible to do).
Hope this helps, Tobias
} } Hi, } } Happy New Year!! } } Has anyone embedded pine needles in JB-4 and sectioned for LM? I had } problem with orientation when I used plastic beem capsules before. I } have been trying silicon rubber molds under 10psi. But it doesn't } polymerize competely. What is the max psi i can go not to damage both } tissue and plastic. } I have been also thinking of switching to LR-white. } But I dont know if i get better result. I would appreciate any kind of } advice on this. } } Thanks. } } Sau Silwal }
Some methycrylates have significant advantages over LR White. We routinely use a mixture of butyl and methylmethacrylate (BMMA) that we got from a paper by Tobias Baskin. This resin can be removed from sections using acetone in a manner analogous to xylene treatment of paraffin sections. This greatly increases immunostaining. Unlike JB-4, this resin easily cuts on water filled troughs. Since it is made from generic methacrylate resins, it is less expensive than the proprietary formulations. It is no good for TEM but you can't have everything. For TEM immunocytochemistry, I prefer LR Gold to LR White since I feel it cuts better with similar immunoreactivity.
-----Original Message----- X-from: Andrew.Bowling-at-ARS.USDA.GOV [mailto:Andrew.Bowling-at-ARS.USDA.GOV] Sent: Saturday, January 05, 2008 1:14 PM To: Phillips, Thomas E.
Sal,
1.) I would definitely switch to LR white. We never use methacrylate resins anymore.
2.) The flat-bottomed capsules recommended by Tobias are much better for polymerizing LR white than BEEM-type capsules. The walls seem to be thicker and are therefore less permeable to oxygen. Also, they are offered in polypropylene. The PP capsules are really a pain to get the blocks out of, but they polymerize really nicely.
3.) I was told that putting LR white (and maybe methacrylate?) resins under vacuum can cause the initiator to evaporate from the resin, causing incomplete polymerization. If you need to vacuum to remove air from your needles, I would do it during fixation or during the post-fixation wash. If you want further details, just email me.
Have fun,
Andy Bowling
-----Original Message----- X-from: baskin-at-bio.umass.edu [mailto:baskin-at-bio.umass.edu] Sent: Wednesday, January 02, 2008 3:50 PM To: Bowling, Andrew
Sau, Not sure exactly if this will solve your problem, but for what its worth, you can get capsules that are like BEEMs except that they have a completely flat bottom. They are made by TAAB but available from major supply houses (no finanical connection). This gives a nice 7 mm (or so) flat surface. You can also get this out of a standard BEEM by flipping it upside down, although you have to mess about to keep the resin from leaking (definitely a mess, but possible to do).
Hope this helps, Tobias
} } Hi, } } Happy New Year!! } } Has anyone embedded pine needles in JB-4 and sectioned for LM? I had } problem with orientation when I used plastic beem capsules before. I } have been trying silicon rubber molds under 10psi. But it doesn't } polymerize competely. What is the max psi i can go not to damage both } tissue and plastic. } I have been also thinking of switching to LR-white. } But I dont know if i get better result. I would appreciate any kind of } advice on this. } } Thanks. } } Sau Silwal }
Marissa, If it turns out that your chamber is glass (which I suspect), after you get it clean, buy yourself a can of unscented White Rain hairspray and spray the inside surface of the glass tube (I would hesitate to do this to plastic because of the possibility of incompatible solvents). The next time you want to clean, just place the glass in hot soapy water. When the hairspray dissolves, your Au/Pd will also depart with little or no effort, and no scratches or cut fingers.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: edelmare-at-muohio.edu [mailto:edelmare-at-muohio.edu] Sent: Friday, January 04, 2008 1:02 PM To: kenconverse-at-qualityimages.biz
Plastic encasing? Are you sure? Our Denton Desk II has a glass cylinder, which we regualrly clean (the inside) of simply but carefully running a new (cleaned) razor blade around. Slides on the glass and peels of the metal. We remove the L-seals, and then we use an ethanol soaked cotton coth to remove any remaining bits and finger Prints. The aluminium bits we polish with a "Green-Scratchey-thing" (3M) and more ethanol, then wiped down with toweling.
The only "Plastic" is the teflon bits inside, and again we use ethanol and paper towels or cotton cloth to polish them up. The case work is painted metals and we use general purpose glass/surface cleaner on it.
On 3 Jan 2008 at 14:58, mlibbee-at-gmail.com wrote:
} } } } ---------------------------------------------------------------------- } ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver using } the WWW based Form at http://www.microscopy.com/MLFormMail.html } ---------------------------------------------------------------------- } ----- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both mlibbee-at-gmail.com as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: mlibbee-at-gmail.com } Name: Marissa } } Title-Subject: [Filtered] Denton Desk II Maintenance } } Question: Greetings! I want to clean up the Denton Desk II sputter } coater (equipped with AuPd target) in my lab facility. Does anyone } have any suggestions as to how I to clean the plastic encasing? Are } there any solvents safe to use for this purpose? } } Thanks and Happy New Year!! } } Marissa } } Login Host: 63.163.107.100 } ---------------------------------------------------------------------- } ----- } } ==============================Original } Headers============================== } 7, 11 -- From zaluzec-at-microscopy.com Thu Jan 3 13:58:09 2008 } 7, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 7, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m03Jw9hA001680 } 7, 11 -- for {microscopy-at-microscopy.com} ; Thu, 3 Jan 2008 13:58:09 -0600 } 7, 11 -- Mime-Version: 1.0 } 7, 11 -- Message-Id: {p06240801c3a2eec41505-at-[206.69.208.22]} } 7, 11 -- Date: Thu, 3 Jan 2008 13:58:07 -0600 } 7, 11 -- To: microscopy-at-microscopy.com } 7, 11 -- From: mlibbee-at-gmail.com (by way of MicroscopyListserver) } 7, 11 -- Subject: viaWWW: Denton Desk II Maintenance } 7, 11 -- Content-Type: text/plain; charset="us-ascii" } ==============================End of - Headers==============================
Richard E. Edelmann, Ph.D. Electron Microscopy Facility Director 364 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.emf.muohio.edu
==============================Original Headers============================== 10, 25 -- From edelmare-at-muohio.edu Fri Jan 4 11:57:15 2008 10, 25 -- Received: from mulnx12.mcs.muohio.edu (mulnx12.mcs.muohio.edu [134.53.6.67]) 10, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m04HvF9R021636 10, 25 -- for {microscopy-at-Microscopy.com} ; Fri, 4 Jan 2008 11:57:15 -0600 10, 25 -- Received: from mulnx23.mcs.muohio.edu (mulnx23.mcs.muohio.edu [134.53.6.10]) 10, 25 -- by mulnx12.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id m04HulEV020683; 10, 25 -- Fri, 4 Jan 2008 12:56:47 -0500 10, 25 -- Received: from [192.168.1.23] ([134.53.14.105]) 10, 25 -- by mulnx23.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id m04Hukvd000531; 10, 25 -- Fri, 4 Jan 2008 12:56:46 -0500 10, 25 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} 10, 25 -- To: "mlibbee-at-gmail.com" {mlibbee-at-gmail.com} 10, 25 -- Date: Fri, 04 Jan 2008 12:56:46 -0500 10, 25 -- MIME-Version: 1.0 10, 25 -- Subject: Re: [Microscopy] viaWWW: Denton Desk II Maintenance 10, 25 -- CC: microscopy-at-Microscopy.com 10, 25 -- Message-ID: {477E2D0E.8384.5D96491-at-edelmare.muohio.edu} 10, 25 -- Priority: normal 10, 25 -- In-reply-to: {200801031958.m03JwshZ003654-at-ns.microscopy.com} 10, 25 -- References: {200801031958.m03JwshZ003654-at-ns.microscopy.com} 10, 25 -- X-mailer: Pegasus Mail for Windows (4.41) 10, 25 -- Content-type: text/plain; charset=US-ASCII 10, 25 -- Content-transfer-encoding: 7BIT 10, 25 -- Content-description: Mail message body 10, 25 -- X-Scanned-By: MIMEDefang 2.57 on 134.53.6.67 ==============================End of - Headers==============================
==============================Original Headers============================== 25, 26 -- From kenconverse-at-qualityimages.biz Sat Jan 5 19:28:32 2008 25, 26 -- Received: from dpmailmta05.doteasy.com (dpmailmta05-14.doteasy.com [65.61.218.94]) 25, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m061SWeY031404 25, 26 -- for {microscopy-at-msa.microscopy.com} ; Sat, 5 Jan 2008 19:28:32 -0600 25, 26 -- Received: from dpmail16.doteasy.com (unverified [192.168.101.16]) 25, 26 -- by dpmailmta05.doteasy.com (DEO) with ESMTP id 20210645-1814644 25, 26 -- for {microscopy-at-msa.microscopy.com} ; Sat, 05 Jan 2008 17:27:43 -0800 25, 26 -- Received: from cpe-72-227-100-25.maine.res.rr.com [72.227.100.25] by dpmail16.doteasy.com with SMTP; 25, 26 -- Sat, 5 Jan 2008 17:28:12 -0800 25, 26 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 25, 26 -- To: "MSA Listserver" {Microscopy-at-msa.microscopy.com} 25, 26 -- Subject: RE: [Microscopy] Re: viaWWW: Denton Desk II Maintenance 25, 26 -- Date: Sat, 5 Jan 2008 20:28:07 -0500 25, 26 -- Message-ID: {000c01c85003$65155540$6401a8c0-at-Ken} 25, 26 -- MIME-Version: 1.0 25, 26 -- Content-Type: text/plain; 25, 26 -- charset="us-ascii" 25, 26 -- X-Priority: 3 (Normal) 25, 26 -- X-MSMail-Priority: Normal 25, 26 -- X-Mailer: Microsoft Outlook, Build 10.0.6822 25, 26 -- Importance: Normal 25, 26 -- Thread-Index: AchO+9setx1O1FT6Tjaet3pJxiYZJQBBqjkg 25, 26 -- In-Reply-To: {200801041801.m04I1e4q026009-at-ns.microscopy.com} 25, 26 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 25, 26 -- Content-Transfer-Encoding: 8bit 25, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m061SWeY031404 ==============================End of - Headers==============================
You are invited to attend our upcoming live, interactive, web-based instructional microscopy seminars.
Pre-registration is required and connection lines are limited so reserve yours now. There is no charge to participate.
See further descriptions and pre-register at: http://www.magbiosystems.com/education
Coming up this week (January 7 - 11):
========================================================== "Quantitative Image and Data Acquisition for Fluorescent Specimens" Advice from a Facility Director
Presented by Brian Matsumoto, Ph.D., University of California, Santa Barbara
I recommend Adobe Photoshop with plug-in Image Processing Tool Kit or FoveaPro, both from Reindeer Graphics for your image analysis needs.
Regards,
Gary M. Brown Microscopy Specialist ExxonMobil Chemical Company Baytown Technology & Engineering - West 5200 Bayway Drive Baytown, Texas 77520-2101 phone: 281 834 2387 fax: 281 834 2395 e-mail: Gary.M.Brown-at-ExxonMobil.com
"Our business in life is not to succeed, but to continue to fail in good spirits." Robert Louis Stevenson
rao5-at-hotmail.c om To gary.m.brown-at-exxonmobil.com 12/26/07 09:14 cc AM Subject [Microscopy] viaWWW: Image Please respond processing software to rao5-at-hotmail.c om
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Question: Hi All, Greetings for Merry X-mas and New Year.... Is there any general purpose image processing software that can do grain size analysis? I like to process AFM, SEM and TEM images eithr in their native format or as bitmaps.
Also, do you recommend any software for processing Selective area diffraction patterns? currently I write IDL code again, not very easy for students to use.
In both cases the software is intended to be used for use in teaching & research both. Price is an important concern
This Question was submitted to Ask-A-Microscopist by (pmccurdy-at-lamar.colostate.edu) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Monday, January 7, 2008 at 12:51:06 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both pmccurdy-at-lamar.colostate.edu as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Email: pmccurdy-at-lamar.colostate.edu Name: Pat McCurdy
Organization: Colorado State University
Education: Graduate College
Location: Fort Collins, Colorado, USA
Title: Cleaning a Moly aperture
Question: Is it possible to use an argon ion gun to clean a moly SEM aperture? If not, why not?
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Email: andrea-at-ncmir.ucsd.edu Name: Andrea Thor
Organization: UCSD
Title-Subject: [Filtered] problems cutting serial thick sections (above 1-2 microns) without getting serious pitting of the blockface
Question: I am having some problems in cutting serial thick sections (above 1-2 microns) without getting serious pitting of the blockface. I heard this is not uncommon when pushing the section thickness to the extreme (such as 3-5 microns). We usually deal with the situation by refacing the block after each thick section, but this of course would make true "serial" sectioning impossible. The blocks I am working with are either cardic left ventricle or striatal tissue embedded in Durcupan.
If anyone has tried or has a protocol or techniques, I'd be very grateful to hear about them. It could save us tons of time and frustration as we develop a new set of protocols.
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Email: levilr-at-hughes.net Name: Lee Levine
Organization: Consultant
Title-Subject: [Filtered] Goniometer eyepiece
Question: I saw a simple goniometer eyepiece for measuring contact angle. Does anyone know who sells these?
Hello Andrea, What kind of knife are you using? I get good results cutting serial thick sections of resin embedded tissues from 1 to 5 microns with a Diatome Diamond Histo-Knife. I have never experienced "pitting" of the block face. I have my own bag of tricks for collecting sections as they sometimes curl up like a wood shaving as they come off the knife especially the thicker ones. Dean Abel Biological Sciences University of Iowa Iowa City IA 52242
At 03:56 PM 1/7/2008, you wrote:
} Email: andrea-at-ncmir.ucsd.edu } Name: Andrea Thor } Organization: UCSD } } Title-Subject: problems cutting serial thick sections (above 1-2 } microns) without getting serious pitting of the blockface } } Question: I am having some problems in cutting serial thick sections } (above 1-2 microns) without getting serious pitting of the } blockface. I heard this is not uncommon when pushing the section } thickness to the extreme (such as 3-5 microns). We usually deal } with the situation by refacing the block after each thick section, } but this of course would make true "serial" sectioning impossible. } The blocks I am working with are either cardic left ventricle or } striatal tissue embedded in Durcupan. } } If anyone has tried or has a protocol or techniques, I'd be very } grateful to hear about them. It could save us tons of time and } frustration as we develop a new set of protocols. Thanks very much. } } Andrea
==============================Original Headers============================== 5, 22 -- From dean-abel-at-uiowa.edu Mon Jan 7 16:51:32 2008 5, 22 -- Received: from itsnt171.iowa.uiowa.edu (itsnt171.iowa.uiowa.edu [128.255.83.12]) 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m07MpWGB000837 5, 22 -- for {Microscopy-at-msa.microscopy.com} ; Mon, 7 Jan 2008 16:51:32 -0600 5, 22 -- Received: from itsnt316.iowa.uiowa.edu ([128.255.62.155]) by itsnt171.iowa.uiowa.edu with Microsoft SMTPSVC(6.0.3790.3959); 5, 22 -- Mon, 7 Jan 2008 16:51:31 -0600 5, 22 -- Received: from abel01.uiowa.edu (128.255.62.21) by email.uiowa.edu 5, 22 -- (128.255.62.155) with Microsoft SMTP Server (TLS) id 8.0.744.0; Mon, 7 Jan 5, 22 -- 2008 16:51:30 -0600 5, 22 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 5, 22 -- Date: Mon, 7 Jan 2008 16:51:33 -0600 5, 22 -- To: Andrea Thor {andrea-at-ncmir.ucsd.edu} , 5, 22 -- Microscopy Society of America 5, 22 -- {Microscopy-at-msa.microscopy.com} 5, 22 -- From: Dean Abel {dean-abel-at-uiowa.edu} 5, 22 -- Subject: Re: [Microscopy]: problems cutting serial thick sections 5, 22 -- In-Reply-To: {200801072156.m07LuKJ6002422-at-ns.microscopy.com} 5, 22 -- References: {200801072156.m07LuKJ6002422-at-ns.microscopy.com} 5, 22 -- MIME-Version: 1.0 5, 22 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 5, 22 -- Message-ID: {ef904fdf-3d96-45de-8040-d01d1f15cef8} 5, 22 -- X-OriginalArrivalTime: 07 Jan 2008 22:51:31.0301 (UTC) FILETIME=[D8614950:01C8517F] ==============================End of - Headers==============================
Instead of using "Technovit 3040", I am looking for an alternative glue for methyl methacrylate (Technovit 9100 Embedding Medium).
Any suggestions would be appreciated.
What I need is: - a mounting medium/glue/adhesive of some sort to attach metal stubs to specimen which have been embedded in methyl methacrylate (Technovit 9100 Embedding Medium).
The company has suggested Technovit* 3040 which: (1) is a yellow, fast-curing (5-10 minutes, depending on room temperature) methyl methacrylate-based resin.
(2) has a "chemical composition that warrants a firm, durable bond with Technovit and secure fixing of the specimens to the Histobloc."
(3) consists of "two components- powder and liquid- allowing simple mixing, easy adhering to the specimen, and fast curing. For fixing the mounts, a highly viscous consistency (i.e., a mixing ratio of approximately 2-3 parts per volume powder: 1 part per volume liquid) has proven to be the most advantageous."
Thanks in advance,
Fanny Chu Ultrastructural Imaging The James C Hogg iCAPTURE Lab, University of British Columbia, St. Paul's Hospital Site Rm 166, 1081 Burrard St, Vancouver, BC, Canada (604) 806-8346, x62712, x62703
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==============================Original Headers============================== 11, 17 -- From FChu-at-mrl.ubc.ca Mon Jan 7 17:34:00 2008 11, 17 -- Received: from mail.mrl.ubc.ca (mail.mrl.ubc.ca [137.82.67.155]) 11, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m07NXwGv014331 11, 17 -- for {Microscopy-at-microscopy.com} ; Mon, 7 Jan 2008 17:33:59 -0600 11, 17 -- Received: from mrlmail-MTA by mail.mrl.ubc.ca 11, 17 -- with Novell_GroupWise; Mon, 07 Jan 2008 15:33:33 -0800 11, 17 -- Message-Id: {4782462A.EB79.00BC.0-at-mrl.ubc.ca} 11, 17 -- X-Mailer: Novell GroupWise Internet Agent 7.0.1 11, 17 -- Date: Mon, 07 Jan 2008 15:32:59 -0800 11, 17 -- From: "Fanny Chu" {FChu-at-mrl.ubc.ca} 11, 17 -- To: {Microscopy-at-microscopy.com} 11, 17 -- Subject: Adhesive for methyl methacrylate (Technovit 9100 Embedding 11, 17 -- Medium) 11, 17 -- Mime-Version: 1.0 11, 17 -- Content-Type: text/plain; charset=ISO-8859-15 11, 17 -- Content-Transfer-Encoding: 8bit 11, 17 -- Content-Disposition: inline ==============================End of - Headers==============================
Hi, For what its worth, I have glued methacrylate blocks (mixtures of methyl and butyl) to epoxy stubs with cyanoacrylate type adhesives (brand name Krazy Glue, among others). THese adhesives are known in general to stick to metal, and they are cheap and available at any hardware store. Might work?
In this case, I hope you *do* get stuck! 8--).
Tobias
} } } Instead of using "Technovit 3040", I am looking for an alternative glue } for methyl methacrylate (Technovit 9100 Embedding Medium). } } Any suggestions would be appreciated. } } What I need is: } - a mounting medium/glue/adhesive of some sort to attach metal stubs to } specimen which have been embedded in methyl methacrylate (Technovit 9100 } Embedding Medium). } } The company has suggested Technovit* 3040 which: } (1) is a yellow, fast-curing (5-10 minutes, depending on room } temperature) methyl methacrylate-based resin. } } (2) has a "chemical composition that warrants a firm, durable bond with } Technovit and secure fixing of the specimens to the Histobloc." } } (3) consists of "two components- powder and liquid- allowing simple } mixing, easy adhering to the specimen, and fast curing. For fixing the } mounts, a highly viscous consistency (i.e., a mixing ratio of } approximately 2-3 parts per volume powder: 1 part per volume liquid) has } proven to be the most advantageous." } } Thanks in advance, } } Fanny Chu } Ultrastructural Imaging } The James C Hogg iCAPTURE Lab, } University of British Columbia, } St. Paul's Hospital Site } Rm 166, 1081 Burrard St, } Vancouver, BC, Canada } (604) 806-8346, x62712, x62703 }
My bean counters are at it again. I have been asked to look into the ways other labs charge for using equipment.
We have always charged by the hour as recorded on a meter that runs when the filament is on. Minimum charge is 0.1 hour. No charges for using specimen prep equipment or for my time other than the cost of consumable supplies.
They are asking about things like minimum charges, like 1 hour minimum to start, charging based on time and extent of 'room' use, charging a portion of my salary in addition to filament time, etc.
It is pretty much a brainstorming activity for now, so anything goes.
Jon
--
Jonathan Krupp Microscopy & Imaging Lab C230 Earth & Marine Science University of California Santa Cruz, CA 95064 (831) 459-2477 jmkrupp-at-ucsc.edu
I rode in AIDS/Lifecycle 6 SF to LA June 3 - 9, 2007 to raise money for the San Francisco AIDS Foundation. Visit http://www.aidslifecycle.org for more information about the ride.
==============================Original Headers============================== 10, 20 -- From jmkrupp-at-ucsc.edu Mon Jan 7 18:10:22 2008 10, 20 -- Received: from ucsc.edu (massmail.ucsc.edu [128.114.129.84]) 10, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m080AMO1007324 10, 20 -- for {microscopy-at-microscopy.com} ; Mon, 7 Jan 2008 18:10:22 -0600 10, 20 -- Received: from [128.114.125.120] (HELO ucsc.edu) 10, 20 -- by massmail.ucsc.edu (CommuniGate Pro SMTP 5.1.7) 10, 20 -- with ESMTPS id 28141835 for microscopy-at-microscopy.com; Mon, 07 Jan 2008 16:10:17 -0800 10, 20 -- Received: by email-prod-fe-1.ucsc.edu (CommuniGate Pro PIPE 5.1.11) 10, 20 -- with PIPE id 39555430; Mon, 07 Jan 2008 16:10:16 -0800 10, 20 -- X-UCSC-EDU-ClamAVCheck: not spam, ClamAV(signaure=none) 10, 20 -- Received: from [128.114.25.127] (account jmkrupp-at-ucsc.edu HELO [128.114.25.127]) 10, 20 -- by email-prod-fe-1.ucsc.edu (CommuniGate Pro SMTP 5.1.11) 10, 20 -- with ESMTPA id 39555356 for microscopy-at-microscopy.com; Mon, 07 Jan 2008 16:10:00 -0800 10, 20 -- Mime-Version: 1.0 10, 20 -- Message-Id: {p06230905c3a86e0bb434-at-[128.114.25.127]} 10, 20 -- Date: Mon, 7 Jan 2008 16:09:58 -0800 10, 20 -- To: microscopy-at-microscopy.com 10, 20 -- From: Jon Krupp {jmkrupp-at-ucsc.edu} 10, 20 -- Subject: How do you charge for use? 10, 20 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
Overview: Schafer Corporation, Sunol, CA, is seeking a skilled and innovative Staff Engineer / Scientist to add to our mass spectrometry group. SVL performs materials characterization and related analytical services on commercial and government contracts. The activities of the group include chemical and elemental analysis of materials on a production basis, maintenance of several mass spectrometers and ancillary equipment, development of new and improved analysis techniques, interpretation, and quality assurance of analytical data. The successful candidate will primarily write software, operate instruments, perform QC/QA, reduce, and analyze data.
Responsibilities: Primary duties include scientific analytical programming, instrument operation and maintenance, developing and optimizing analytical techniques, statistical analysis, and data interpretation. Will prepare and make presentations on technical findings. As a lead engineer or scientist, this individual will be a key resource for Schafer and our customers, maintaining our expertise in the field of high sensitivity, high precision isotopic analysis.
Qualifications: The ideal candidate must have a strong background in materials science or engineering, or a related field of physics, geology, chemistry, statistics, or mathematics. Should have experience in a scientific laboratory, preferably operating SIMS, TIMS, SEM, or TEM. Experience with high vacuum technology, electronics, mechanical design, cryogenic system, and ion optics is helpful. Should have demonstrated scientific programming experience (such as Labview or FORTRAN), including statistical analysis and data interpretation. Database familiarity is helpful. Must be able to work independently and as part of a team of scientists, engineers, and technicians. Good customer service focus and commitment to quality are required.
Other qualifications include: • Bachelor's degree in physical science or engineering with minimum 5 years of technical experience. • Demonstrated ability to perform complex professional engineering / scientific work, including scientific analysis, planning, and execution. • Analytical laboratory experience in software, statistics, data evaluation, and quality control. • Proven ability to clearly write and present scientific reports and proposals. • Must be a US citizen with the ability to obtain government security clearance.
Apply at: http://jobs-schafer.icims.com/schafer_jobs/jobs/candidate/job.jsp?jobid=1331 &mode=view Schafer Corporation is an Affirmative Action, Equal Opportunity Employer.
David Sams, Ph.D. Group Leader, Mass Spectrometry AEM Group Schafer Laboratories 6705 Vallecitos Rd. Sunol, CA 94586 dsams-at-schaferlabs.com
==============================Original Headers============================== 8, 20 -- From dsams-at-schaferlabs.com Mon Jan 7 18:37:27 2008 8, 20 -- Received: from mail.schaferlabs.com (mx1.schaferlabs.com [64.168.91.154]) 8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m080bQBS020270 8, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 7 Jan 2008 18:37:26 -0600 8, 20 -- Received: from dsamsws ([10.10.10.1]) 8, 20 -- by mail.schaferlabs.com (8.12.11.20060308/8.12.11) with ESMTP id m080aKdq028197 8, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 7 Jan 2008 16:36:20 -0800 8, 20 -- Message-Id: {200801080036.m080aKdq028197-at-mail.schaferlabs.com} 8, 20 -- From: "David Sams" {dsams-at-schaferlabs.com} 8, 20 -- To: {Microscopy-at-microscopy.com} 8, 20 -- Subject: Job Posting: Mass Spectrometry Staff Engineer / Scientist 8, 20 -- Date: Mon, 7 Jan 2008 16:37:27 -0800 8, 20 -- MIME-Version: 1.0 8, 20 -- Content-Type: text/plain; 8, 20 -- charset="iso-8859-1" 8, 20 -- X-Mailer: Microsoft Office Outlook, Build 11.0.6353 8, 20 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 8, 20 -- Thread-Index: AchRjqTg+nCEQmc9SX2ktcftfgYhrg== 8, 20 -- Content-Transfer-Encoding: 8bit 8, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m080bQBS020270 ==============================End of - Headers==============================
We used to do this, indeed, we were supposed to operate under "full cost recovery". However, in their wisdom(?), the corporate types in our organisation decided that any "unit" with operational costs (including salaries) less than $1 million per year could stop charging like this and be funded entirely from overheads. We still have to charge outsiders, and still record instrument use, but no more charging. From one extreme to the other.....
cheers, rosemary
Rosemary White rosemary.white-at-csiro.au CSIRO Plant Industry ph. 61 (0)2-6246 5475 GPO Box 1600 fax. 61 (0)2-6246 5334 Canberra, ACT 2601 Australia
On 8/1/08 11:15 AM, "jmkrupp-at-ucsc.edu" {jmkrupp-at-ucsc.edu} wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi: } } My bean counters are at it again. I have been asked to look into the } ways other labs charge for using equipment. } } We have always charged by the hour as recorded on a meter that runs } when the filament is on. Minimum charge is 0.1 hour. No charges for } using specimen prep equipment or for my time other than the cost of } consumable supplies. } } They are asking about things like minimum charges, like 1 hour } minimum to start, charging based on time and extent of 'room' use, } charging a portion of my salary in addition to filament time, etc. } } It is pretty much a brainstorming activity for now, so anything goes. } } Jon }
==============================Original Headers============================== 9, 25 -- From prvs=Rosemary.White=886c556c5-at-csiro.au Mon Jan 7 20:50:42 2008 9, 25 -- Received: from act-MTAout1.csiro.au (act-MTAout1.csiro.au [150.229.7.37]) 9, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m082oeEm003539 9, 25 -- for {microscopy-at-microscopy.com} ; Mon, 7 Jan 2008 20:50:41 -0600 9, 25 -- X-IronPort-AV: E=Sophos;i="4.24,255,1196600400"; 9, 25 -- d="scan'208";a="183307521" 9, 25 -- Received: from exgw1-cbr.nexus.csiro.au ([152.83.3.66]) 9, 25 -- by act-ironport-int.csiro.au with ESMTP; 08 Jan 2008 13:50:39 +1100 9, 25 -- Received: from EXACTN1-CBR.nexus.csiro.au ([152.83.131.131]) by exgw1-cbr.nexus.csiro.au with Microsoft SMTPSVC(5.0.2195.6713); 9, 25 -- Tue, 8 Jan 2008 13:50:38 +1100 9, 25 -- Received: OWA.CSIRO.AU 152.83.131.131 from 138.194.3.58 138.194.3.58 via HTTP with MS-WebStorage 6.0.6249 9, 25 -- Received: 138.194.3.58 138.194.3.58 from via HTTP with MS-WebStorage 6.0.6249 9, 25 -- User-Agent: Microsoft-Entourage/11.0.0.040405 9, 25 -- Date: Tue, 08 Jan 2008 13:54:23 +1100 9, 25 -- Subject: Re: [Microscopy] How do you charge for use? 9, 25 -- From: Rosemary White {Rosemary.White-at-csiro.au} 9, 25 -- To: {jmkrupp-at-ucsc.edu} 9, 25 -- CC: {microscopy-at-microscopy.com} 9, 25 -- Message-ID: {C3A9310F.836B%Rosemary.White-at-csiro.au} 9, 25 -- In-Reply-To: {200801080015.m080F9aY017467-at-ns.microscopy.com} 9, 25 -- Mime-version: 1.0 9, 25 -- Content-type: text/plain; 9, 25 -- charset="US-ASCII" 9, 25 -- Content-transfer-encoding: 7bit 9, 25 -- X-OriginalArrivalTime: 08 Jan 2008 02:50:38.0856 (UTC) FILETIME=[40307080:01C851A1] ==============================End of - Headers==============================
I have no experience with the Coolwell cooler that you mention but the symptoms sound similar to our UK Flowcool cooler.
After 2 or 3 years the temperature readout becomes erratic and when I contacted the supplier/manufacturer they told me to replace the temperature probe in the water tank. They have supplied me with spares and on my machine it is a relatively simple operation.
If you can still contact the manufacturer maybe they can help.
Good luck.
Malcolm
Malcolm Haswell e.m. unit Chemispec School of Health, Natural and Social Sciences Fleming Building University of Sunderland Tyne & Wear SR1 3SD UK e-mail: malcolm.haswell-at-sunderland.ac.uk
----- Original Message ----- X-from: Jill.Verlander-at-medicine.ufl.edu
Hi Andrea,
Briefly, this type of problem is down to brittle resin blocks being sectioned at too fast a cutting speed and the thicker the section, the more likely it is to occur.
Try adjusting the hardener/plasticiser proportion of your embedding mix and/or reducing the polymerisation time/temperature to end up with a softer block.
With the blocks already processed, use the best available knife and reduce the cutting speed. If the chipping of the face recurs, your only remaining option is to reduce the section thickness.
Like Dean Abel suggests, I would be using a histodiamond (wet) with a cutting window speed of 1mm/sec and increasing the section thickness gradually from an initial 2 microns. I would also trim off most of the surrounding resin and shape the block to a point to reduce the cutting force on the knife, particularly as you are wanting to section at up to 5 microns.
Happy New Year!
Alastair McKinnon Histology & EM Facility Manager University of Aberdeen, Institute of Medical Science Foresterhill, Aberdeen, Scotland, AB25 2ZD tel: +44(0)1224 552923 (office); +44(0)1224 555911 (lab) fax: +44(0)1224 559533; email: a.d.mckinnon-at-abdn.ac.uk www.abdn.ac.uk/ims/h-em -----Original Message----- X-from: andrea-at-ncmir.ucsd.edu [mailto:andrea-at-ncmir.ucsd.edu] Sent: 07 January 2008 22:02 To: Mckinnon, Alastair D.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at ------------------------------------------------------------------------ --- Remember this posting is most likely not from a Subscriber, so when replying please copy both andrea-at-ncmir.ucsd.edu as well as the MIcroscopy Listserver ------------------------------------------------------------------------ ---
Email: andrea-at-ncmir.ucsd.edu Name: Andrea Thor
Organization: UCSD
Title-Subject: [Filtered] problems cutting serial thick sections (above 1-2 microns) without getting serious pitting of the blockface
Question: I am having some problems in cutting serial thick sections (above 1-2 microns) without getting serious pitting of the blockface. I heard this is not uncommon when pushing the section thickness to the extreme (such as 3-5 microns). We usually deal with the situation by refacing the block after each thick section, but this of course would make true "serial" sectioning impossible. The blocks I am working with are either cardic left ventricle or striatal tissue embedded in Durcupan.
If anyone has tried or has a protocol or techniques, I'd be very grateful to hear about them. It could save us tons of time and frustration as we develop a new set of protocols.
Hi Jon, We charge per/hour for equipment use: TEM, SEM, CPD, Sputter Coater, Digital LM and any dark room use (yes there are still a couple of researches using film!!).We do not allow users to process their samples here as there is not enough room nor do we train them. We can process the tissue for them at a price per every 2 samples (we figure an experimental and a control is pretty standard). This processing charge includes supplies and our time. We then charge per block for thick sections and another charge for thin sectioning. Our time is charged at rates that take into consideration who's hands are helping/training: SRA I, SRAII or SRAIV. We do have a few supplies we will sell to the researcher at our cost (tax and shipping figured into the equation): stubs, coated grids, 16% paraformaldehyde, etc. Hope this helps! Pat Kysar University of California, Davis Medical School, Pathology EM Lab 530-752-4701
----- Original Message ----- X-from: {jmkrupp-at-ucsc.edu} To: {pekysar-at-ucdavis.edu} Sent: Monday, January 07, 2008 4:15 PM
Dear Microscopists,
The first update of the list of meetings for microscopists in the year 2008 has been finished at the Petr's Microscopy Resources at the
Your submission of other meetings will be very appreciated (submission form is accessible from the page mentioned, the direct link for the submission is http://www.petr.isibrno.cz/microscopy/PMRform.php).
Regards, Petr Schauer ******************************************** Dr. Petr Schauer Scintillation and Cathodoluminescent Systems Institute of Scientific Instruments, AS CR, v.v.i. Academy of Sciences of the Czech Republic Kralovopolska 147, CZ-61264 Brno, Czech Republic ******************************************** tel.: +420 541 514 313 fax : +420 541 514 404, or +420 541 514 402 e-mail: petr-at-isibrno.cz www: http://www.petr.isibrno.cz/ ********************************************
==============================Original Headers============================== 6, 18 -- From PetrS-at-ISIBrno.Cz Wed Jan 9 08:39:46 2008 6, 18 -- Received: from pecka.isibrno.cz (pecka.isibrno.cz [195.178.70.10]) 6, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m09EdhBT031987 6, 18 -- for {Microscopy-at-Microscopy.Com} ; Wed, 9 Jan 2008 08:39:45 -0600 6, 18 -- Received: from [195.178.70.170] (aligator.isibrno.cz [195.178.70.170]) 6, 18 -- by pecka.isibrno.cz (8.14.1/8.14.1) with ESMTP id m09EdPk3022178 6, 18 -- for {Microscopy-at-Microscopy.Com} ; Wed, 9 Jan 2008 15:39:25 +0100 (CET) 6, 18 -- (envelope-from PetrS-at-ISIBrno.Cz) 6, 18 -- Message-ID: {4784DC99.3060909-at-ISIBrno.Cz} 6, 18 -- Date: Wed, 09 Jan 2008 15:39:21 +0100 6, 18 -- From: Petr Schauer {PetrS-at-ISIBrno.Cz} 6, 18 -- Organization: ISI ASCR Brno 6, 18 -- User-Agent: Thunderbird 2.0.0.9 (Windows/20071031) 6, 18 -- MIME-Version: 1.0 6, 18 -- To: Microscopy ListServer {Microscopy-at-Microscopy.Com} 6, 18 -- Subject: Meetings for Microscopists in 2008 6, 18 -- Content-Type: text/plain; charset=ISO-8859-2; format=flowed 6, 18 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I am looking for a free software to convert eds to emsa extension file.
I am using an old software for Thermo/Noran EDS. It has two types of file format: Vantage (eds extension) and EMSA (emsa extension). Recent NORAN System Six can read emsa format, but not eds format.
I can open eds file using old software and save as emsa format. However, it is inconvenient. Is there any alternative method (like free software) to convert eds format to emsa format? Please advise.
Thank you,
Hiromi Konishi, Ph.D. UW-MADISON
==============================Original Headers============================== 6, 29 -- From hikonishi3-at-gmail.com Wed Jan 9 09:24:37 2008 6, 29 -- Received: from wx-out-0506.google.com (wx-out-0506.google.com [66.249.82.232]) 6, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m09FOX31007588 6, 29 -- for {Microscopy-at-microscopy.com} ; Wed, 9 Jan 2008 09:24:36 -0600 6, 29 -- Received: by wx-out-0506.google.com with SMTP id h30so120951wxd.21 6, 29 -- for {Microscopy-at-microscopy.com} ; Wed, 09 Jan 2008 07:24:31 -0800 (PST) 6, 29 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 6, 29 -- d=gmail.com; s=gamma; 6, 29 -- h=domainkey-signature:received:received:message-id:date:from:sender:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition:x-google-sender-auth; 6, 29 -- bh=FxqSWvI1r65GAqgIcGjCQRehRahdZYVJZHkeOqMso6o=; 6, 29 -- b=XjUM7RL0u5pnygLItvC9PnsZgPRpRn4n6pd1J6WMM0CC0I1rKbGptr+wo+rAV1o2RytREUSLEdG/tcQbDWa7IWjlzjtlAcWJkvZ0eXYV6s//BZsfoQrweQEz3IgVUHlFtCxbdhT+CKpZW/G/9Y6iq4TW1k7HMvINljPRO/9jV+E= 6, 29 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 6, 29 -- d=gmail.com; s=gamma; 6, 29 -- h=message-id:date:from:sender:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition:x-google-sender-auth; 6, 29 -- b=oiMxOou6cmZL1AQAJxFDY4B6plneFMaPknYID6zK2c1jPW1ttAah9C8QMOj6XFeu6NyZgRY8gkPaTFsYo3QSsCwUlj8PrIqNvPNbBNVcUyDMEBVGdWPozyzzgEGAu78bD/8QAeRqosHlNPtES7cGrMheDrqlCUQpUuXqLr2/ZkM= 6, 29 -- Received: by 10.142.102.5 with SMTP id z5mr314726wfb.15.1199892270489; 6, 29 -- Wed, 09 Jan 2008 07:24:30 -0800 (PST) 6, 29 -- Received: by 10.143.6.4 with HTTP; Wed, 9 Jan 2008 07:24:30 -0800 (PST) 6, 29 -- Message-ID: {456d33d00801090724k6f86a0du6643470c3b98a2a6-at-mail.gmail.com} 6, 29 -- Date: Wed, 9 Jan 2008 09:24:30 -0600 6, 29 -- From: "Hiromi Konishi" {hkonishi-at-wisc.edu} 6, 29 -- Sender: hikonishi3-at-gmail.com 6, 29 -- To: Microscopy-at-microscopy.com 6, 29 -- Subject: Thermo/Noran EDS: How to convert eds to emsa file? 6, 29 -- MIME-Version: 1.0 6, 29 -- Content-Type: text/plain; charset=ISO-8859-1 6, 29 -- Content-Transfer-Encoding: 7bit 6, 29 -- Content-Disposition: inline 6, 29 -- X-Google-Sender-Auth: e7e12a6676b9b5ff ==============================End of - Headers==============================
Hiromi, Speak with your Thermo service folks. There is a software application they have that converts .eds format (and all the other Vantage file formats .grey etc.) to formats that are compatible with the System SIX software. It is intended as a one-time only tool to be used during the upgrade from a Vantage system to a System SIX system, but if you speak with their technical service folks, they might be able to work out a solution for you.
Good Luck, Bryan Bandli
hkonishi-at-wisc.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hello: } } I am looking for a free software to convert eds to emsa extension file. } } I am using an old software for Thermo/Noran EDS. It has two types of } file format: Vantage (eds extension) and EMSA (emsa extension). Recent } NORAN System Six can read emsa format, but not eds format. } } I can open eds file using old software and save as emsa format. } However, it is inconvenient. Is there any alternative method (like } free software) to convert eds format to emsa format? Please advise. } } Thank you, } } Hiromi Konishi, Ph.D. } UW-MADISON } } ==============================Original Headers============================== } 6, 29 -- From hikonishi3-at-gmail.com Wed Jan 9 09:24:37 2008 } 6, 29 -- Received: from wx-out-0506.google.com (wx-out-0506.google.com [66.249.82.232]) } 6, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m09FOX31007588 } 6, 29 -- for {Microscopy-at-microscopy.com} ; Wed, 9 Jan 2008 09:24:36 -0600 } 6, 29 -- Received: by wx-out-0506.google.com with SMTP id h30so120951wxd.21 } 6, 29 -- for {Microscopy-at-microscopy.com} ; Wed, 09 Jan 2008 07:24:31 -0800 (PST) } 6, 29 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 6, 29 -- d=gmail.com; s=gamma; } 6, 29 -- h=domainkey-signature:received:received:message-id:date:from:sender:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition:x-google-sender-auth; } 6, 29 -- bh=FxqSWvI1r65GAqgIcGjCQRehRahdZYVJZHkeOqMso6o=; } 6, 29 -- b=XjUM7RL0u5pnygLItvC9PnsZgPRpRn4n6pd1J6WMM0CC0I1rKbGptr+wo+rAV1o2RytREUSLEdG/tcQbDWa7IWjlzjtlAcWJkvZ0eXYV6s//BZsfoQrweQEz3IgVUHlFtCxbdhT+CKpZW/G/9Y6iq4TW1k7HMvINljPRO/9jV+E= } 6, 29 -- DomainKey-Signature: a=rsa-sha1; c=nofws; } 6, 29 -- d=gmail.com; s=gamma; } 6, 29 -- h=message-id:date:from:sender:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition:x-google-sender-auth; } 6, 29 -- b=oiMxOou6cmZL1AQAJxFDY4B6plneFMaPknYID6zK2c1jPW1ttAah9C8QMOj6XFeu6NyZgRY8gkPaTFsYo3QSsCwUlj8PrIqNvPNbBNVcUyDMEBVGdWPozyzzgEGAu78bD/8QAeRqosHlNPtES7cGrMheDrqlCUQpUuXqLr2/ZkM= } 6, 29 -- Received: by 10.142.102.5 with SMTP id z5mr314726wfb.15.1199892270489; } 6, 29 -- Wed, 09 Jan 2008 07:24:30 -0800 (PST) } 6, 29 -- Received: by 10.143.6.4 with HTTP; Wed, 9 Jan 2008 07:24:30 -0800 (PST) } 6, 29 -- Message-ID: {456d33d00801090724k6f86a0du6643470c3b98a2a6-at-mail.gmail.com} } 6, 29 -- Date: Wed, 9 Jan 2008 09:24:30 -0600 } 6, 29 -- From: "Hiromi Konishi" {hkonishi-at-wisc.edu} } 6, 29 -- Sender: hikonishi3-at-gmail.com } 6, 29 -- To: Microscopy-at-microscopy.com } 6, 29 -- Subject: Thermo/Noran EDS: How to convert eds to emsa file? } 6, 29 -- MIME-Version: 1.0 } 6, 29 -- Content-Type: text/plain; charset=ISO-8859-1 } 6, 29 -- Content-Transfer-Encoding: 7bit } 6, 29 -- Content-Disposition: inline } 6, 29 -- X-Google-Sender-Auth: e7e12a6676b9b5ff } ==============================End of - Headers============================== } }
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==============================Original Headers============================== 6, 20 -- From bbandli-at-mvainc.com Wed Jan 9 09:59:25 2008 6, 20 -- Received: from smtp03.atlngahp.sys.nuvox.net (smtp-out3.atlngahp.sys.nuvox.net [70.43.63.20]) 6, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m09FxMxs027503 6, 20 -- for {Microscopy-at-microscopy.com} ; Wed, 9 Jan 2008 09:59:24 -0600 6, 20 -- Received: from [192.168.1.95] (216.215.228.34.nw.nuvox.net [216.215.228.34]) 6, 20 -- by smtp03.atlngahp.sys.nuvox.net (8.13.1/8.13.1) with ESMTP id m09FxFO9003591; 6, 20 -- Wed, 9 Jan 2008 10:59:15 -0500 6, 20 -- Message-ID: {4784EFD7.9050000-at-mvainc.com} 6, 20 -- Date: Wed, 09 Jan 2008 11:01:27 -0500 6, 20 -- From: bbandli {bbandli-at-mvainc.com} 6, 20 -- Reply-To: bbandli-at-mvainc.com 6, 20 -- Organization: MVA Scientific Consultants 6, 20 -- User-Agent: Thunderbird 2.0.0.9 (Windows/20071031) 6, 20 -- MIME-Version: 1.0 6, 20 -- To: hkonishi-at-wisc.edu, Microscopy-at-microscopy.com 6, 20 -- Subject: Re: [Microscopy] Thermo/Noran EDS: How to convert eds to emsa file? 6, 20 -- References: {200801091526.m09FQ5pG008831-at-ns.microscopy.com} 6, 20 -- In-Reply-To: {200801091526.m09FQ5pG008831-at-ns.microscopy.com} 6, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 20 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Is there some one who knows how to calculate number fractions (not length or pixellength fractions) of CSL boundaries (or grainboundaries in general) from TSL and / or HKL Channel Five datasets?
Thanks for thinking along with me,
Corrie ----------------------------------------------------------- Corrie van Hoek
CORUS RD & T Ceramics Research Center Building Code 3J-22 PO Box 1000 1970 CA Ijmuiden The Netherlands tel. 02514 92626 fax. 02514 70489
********************************************************************** This transmission is confidential and must not be used or disclosed by anyone other than the intended recipient. Neither Corus Group Limited nor any of its subsidiaries can accept any responsibility for any use or misuse of the transmission by anyone.
For address and company registration details of certain entities within the Corus group of companies, please visit http://www.corusgroup.com/entities
Hi! During some works in a room next to our TEM, one worker opened a canalisation of the watercooling system, emptying the water tank of the watercooling device. I filled it again with normal tap water but I think I have to add some product to avoid mold growth. Could you recommend one such product and where I can order it, especially those of you from Germany and Austria (we are located in Vienna, Austria)? Our microscope is a Tecnai G20 and the watercooling unit is a Zephy ZEM 1000S.
Thank you in advance, Stephane
____________________________________________________________________________________ Never miss a thing. Make Yahoo your home page. http://www.yahoo.com/r/hs
==============================Original Headers============================== 4, 19 -- From nizets2-at-yahoo.com Thu Jan 10 06:40:52 2008 4, 19 -- Received: from web37403.mail.mud.yahoo.com (web37403.mail.mud.yahoo.com [209.191.91.135]) 4, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m0ACem4n001942 4, 19 -- for {microscopy-at-microscopy.com} ; Thu, 10 Jan 2008 06:40:50 -0600 4, 19 -- Received: (qmail 40247 invoked by uid 60001); 10 Jan 2008 12:40:37 -0000 4, 19 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 4, 19 -- s=s1024; d=yahoo.com; 4, 19 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Message-ID; 4, 19 -- b=Fn3gG5utMTGOd4DpVnbGJeUkMJ1vkheZBOP1Zlai3H7cinr/ZsdrN2y8+5E+BNqTAFvpRmsa+7R7cpvr9Lm5XjgKsb5AtFa1hKi8ajiPOcAevuTeV8DGXVwJ2vzxC7zQMd0X1Vr8LddylbtVm86IZpCLAk6Qsq3vB0sabN3cgcs=; 4, 19 -- X-YMail-OSG: QJ3hsoAVM1l_O63TUbf1U2TeLu.w7OQGeGK7boHrsuxxVDDcpK431mVgc_WIKpM7Fmag2Pq11gv0JaKuQnqAsHtsSe3IQ5PJX6ZT2Jr5GhK0JYN7Cdg- 4, 19 -- Received: from [80.122.101.100] by web37403.mail.mud.yahoo.com via HTTP; Thu, 10 Jan 2008 04:40:37 PST 4, 19 -- X-Mailer: YahooMailRC/818.31 YahooMailWebService/0.7.158.1 4, 19 -- Date: Thu, 10 Jan 2008 04:40:37 -0800 (PST) 4, 19 -- From: Stephane Nizet {nizets2-at-yahoo.com} 4, 19 -- Subject: Product for watercooling 4, 19 -- To: microscopy-at-microscopy.com 4, 19 -- MIME-Version: 1.0 4, 19 -- Content-Type: text/plain; charset=us-ascii 4, 19 -- Message-ID: {403898.39040.qm-at-web37403.mail.mud.yahoo.com} ==============================End of - Headers==============================
We have just inherited a BAL-TEC 060 freeze fracture system.
I would like to ask if anyone out in there Microscopy Land has similar equipment and has written their own set of operation notes for this system that they would be willing to share with us, perhaps as a pdf file or similar?
We have also inherited with the freeze fracture system a Bal-Tec SBU 020 sandblasting Unit. Does anyone have one of these also and be wiling to share the operating notes?
Many thanks, kind regards
Allan
Allan Mitchell Technical Manager Otago Centre for Electron Microscopy Department of Anatomy and Structural Biology School of Medical Sciences P.O. Box 913 Dunedin New Zealand
Phone (03) 479 5642 or 479 7301 Fax (03) 479 5086 or 479 7254
==============================Original Headers============================== 12, 21 -- From allan.mitchell-at-stonebow.otago.ac.nz Thu Jan 10 16:44:05 2008 12, 21 -- Received: from mailhub2.otago.ac.nz (mailhub2.otago.ac.nz [139.80.64.247]) 12, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0AMi3hZ003133 12, 21 -- for {microscopy-at-msa.microscopy.com} ; Thu, 10 Jan 2008 16:44:04 -0600 12, 21 -- Received: from galadriel.otago.ac.nz (galadriel.otago.ac.nz [139.80.64.213]) 12, 21 -- by mailhub2.otago.ac.nz (8.13.8/8.13.8) with ESMTP id m0AMi1eQ013135 12, 21 -- for {microscopy-at-msa.microscopy.com} ; Fri, 11 Jan 2008 11:44:01 +1300 12, 21 -- Received: from allan.otago.ac.nz ([139.80.40.92]) 12, 21 -- by galadriel.otago.ac.nz with esmtp (Exim 4.63) 12, 21 -- (envelope-from {allan.mitchell-at-stonebow.otago.ac.nz} ) 12, 21 -- id 1JD67z-0006uh-Dn 12, 21 -- for microscopy-at-msa.microscopy.com; Fri, 11 Jan 2008 11:43:59 +1300 12, 21 -- Mime-Version: 1.0 (Apple Message framework v752.2) 12, 21 -- Content-Transfer-Encoding: 7bit 12, 21 -- Message-Id: {11C3F12C-3D84-438C-A3D5-637CB20DBFEC-at-stonebow.otago.ac.nz} 12, 21 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 12, 21 -- To: microscopy-at-msa.microscopy.com 12, 21 -- From: Allan Mitchell {allan.mitchell-at-stonebow.otago.ac.nz} 12, 21 -- Subject: Bal-Tec 060 Freeze Etching System 12, 21 -- Date: Fri, 11 Jan 2008 11:44:36 +1300 12, 21 -- X-Mailer: Apple Mail (2.752.2) ==============================End of - Headers==============================
I would suggest draining the water and replacing with distilled water. The Cl in tap water could lead to corrosion of metal in contact with the water. A good additive IMO is Ethylene Glycol as a 10% mix. I use technical grade EG and add 1/2 gallon to the 4.5 gallons of distilled water for 5 gallons total in the Haskris R050 chiller reservoir.
Sometimes this holds up for a year.
gary g.
At 04:52 AM 1/10/2008, you wrote:
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==============================Original Headers============================== 11, 20 -- From gary-at-gaugler.com Thu Jan 10 16:56:56 2008 11, 20 -- Received: from qsmtp4.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 11, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m0AMutZj015411 11, 20 -- for {microscopy-at-microscopy.com} ; Thu, 10 Jan 2008 16:56:56 -0600 11, 20 -- Message-Id: {200801102256.m0AMutZj015411-at-ns.microscopy.com} 11, 20 -- Received: (qmail 7535 invoked from network); 10 Jan 2008 14:56:57 -0800 11, 20 -- Received: by simscan 1.1.0 ppid: 7531, pid: 7533, t: 0.0916s 11, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 11, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 11, 20 -- by qsmtp4 with SMTP; 10 Jan 2008 14:56:57 -0800 11, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 11, 20 -- Date: Thu, 10 Jan 2008 14:56:53 -0800 11, 20 -- To: nizets2-at-yahoo.com 11, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 11, 20 -- Subject: Re: [Microscopy] Product for watercooling 11, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 11, 20 -- In-Reply-To: {200801101252.m0ACqLYm004944-at-ns.microscopy.com} 11, 20 -- References: {200801101252.m0ACqLYm004944-at-ns.microscopy.com} 11, 20 -- Mime-Version: 1.0 11, 20 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-360B2309 ==============================End of - Headers==============================
We incorporate Sigma water bath treatment in the reservoir - 3.5ml per tank full (25L).
Seems to work well - lasting about 12 months and got this recommendation from Sigma Technical Services re using it in chiller circulators for EMs.
TechServ_Number: S5525 TechServ_Name: SigmaClean; water bath treatment TechServ_Brand: SIGMA
TechServ_re: is this product recommended for use in chiller circulators for the supply of cooling water for a transmission EM (Philips CM10).
Best regards,
Alastair
Alastair McKinnon Histology & EM Facility Manager, IMS R2.62 University of Aberdeen, Institute of Medical Science Foresterhill, Aberdeen, AB25 2ZD tel: +44(0)1224 552923 (office); +44(0)1224 555911 (lab) fax: +44(0)1224 559533; email: a.d.mckinnon-at-abdn.ac.uk www.abdn.ac.uk/ims/h-em -----Original Message----- X-from: gary-at-gaugler.com [mailto:gary-at-gaugler.com] Sent: 10 January 2008 23:00 To: Mckinnon, Alastair D.
I would suggest draining the water and replacing with distilled water. The Cl in tap water could lead to corrosion of metal in contact with the water. A good additive IMO is Ethylene Glycol as a 10% mix. I use technical grade EG and add 1/2 gallon to the 4.5 gallons of distilled water for 5 gallons total in the Haskris R050 chiller reservoir.
Sometimes this holds up for a year.
gary g.
At 04:52 AM 1/10/2008, you wrote:
} ----------------------------------------------------------------------- } ----- The Microscopy ListServer -- CoSponsor: The Microscopy Society } of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver
} watercooling device. } I filled it again with normal tap water but I think I have to add some } product to avoid mold growth. } Could you recommend one such product and where I can order it, } especially those of you from Germany and Austria (we are located in } Vienna, Austria)? } Our microscope is a Tecnai G20 and the watercooling unit is a Zephy ZEM 1000S. } } Thank you in advance, } Stephane
==============================Original Headers============================== 11, 20 -- From gary-at-gaugler.com Thu Jan 10 16:56:56 2008 11, 20 -- Received: from qsmtp4.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 11, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m0AMutZj015411 11, 20 -- for {microscopy-at-microscopy.com} ; Thu, 10 Jan 2008 16:56:56 -0600 11, 20 -- Message-Id: {200801102256.m0AMutZj015411-at-ns.microscopy.com} 11, 20 -- Received: (qmail 7535 invoked from network); 10 Jan 2008 14:56:57 -0800 11, 20 -- Received: by simscan 1.1.0 ppid: 7531, pid: 7533, t: 0.0916s 11, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 11, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 11, 20 -- by qsmtp4 with SMTP; 10 Jan 2008 14:56:57 -0800 11, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 11, 20 --
All, After much fighting of corrosion and algae issues I settled years ago on what might be the easiest and most long term "solution". That is pure distilled water. And here's the secret, you don't need to add any chemical additives if you remove all clear plastic hoses from the system and replace them with black or otherwise opaque materials. No light, no growth.
With this "solution" I have found I can run for years with a crystal clear tank and with only an occasional speck of rust visible in the bottom of the tank, which is probably left over from the prior years of running god know what through the lines. This "solution" is currently running in several SEMs and microprobes, using a variety of chillers (mostly Haskris which I have found to be much more reliable than Coolwell).
Works for me anyway. john
At 07:58 AM 1/11/2008, a.d.mckinnon-at-abdn.ac.uk wrote:
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==============================Original Headers============================== 8, 21 -- From donovan-at-uoregon.edu Fri Jan 11 10:37:25 2008 8, 21 -- Received: from smtp.uoregon.edu (mserv1.uoregon.edu [128.223.142.40]) 8, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0BGbOfe005078 8, 21 -- for {microscopy-at-microscopy.com} ; Fri, 11 Jan 2008 10:37:24 -0600 8, 21 -- Received: from Probev.uoregon.edu (probev.uoregon.edu [128.223.92.75]) 8, 21 -- (authenticated bits=0) 8, 21 -- by smtp.uoregon.edu (8.13.8/8.13.8) with ESMTP id m0BGbNgM019167 8, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 8, 21 -- for {microscopy-at-microscopy.com} ; Fri, 11 Jan 2008 08:37:23 -0800 8, 21 -- Message-Id: {200801111637.m0BGbNgM019167-at-smtp.uoregon.edu} 8, 21 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 8, 21 -- Date: Fri, 11 Jan 2008 08:37:13 -0800 8, 21 -- To: microscopy-at-microscopy.com 8, 21 -- From: John Donovan {donovan-at-uoregon.edu} 8, 21 -- Subject: Re: [Microscopy] Product for watercooling 8, 21 -- In-Reply-To: {200801111558.m0BFwJHR032212-at-ns.microscopy.com} 8, 21 -- References: {200801111558.m0BFwJHR032212-at-ns.microscopy.com} 8, 21 -- Mime-Version: 1.0 8, 21 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 8, 21 -- X-Virus-Scanned: ClamAV 0.92/5478/Fri Jan 11 07:39:22 2008 on mserv1 8, 21 -- X-Virus-Status: Clean ==============================End of - Headers==============================
I second John's "solution". It's what we have been using for our FESEM for several years without any problems. Opaque lines from the chiller to the scope are essential however. Even though this is what this scope has been on since day 1, we do have a small amount of rust/grit in the bottom of the tank but it hasn't been a problem. We also change the H2O every 6months (Per JEOL service). Cheers, Bryan Bandli
donovan-at-uoregon.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } All, } After much fighting of corrosion and algae issues I settled years ago } on what might be the easiest and most long term "solution". That is } pure distilled water. And here's the secret, you don't need to add } any chemical additives if you remove all clear plastic hoses from the } system and replace them with black or otherwise opaque materials. No } light, no growth. } } With this "solution" I have found I can run for years with a crystal } clear tank and with only an occasional speck of rust visible in the } bottom of the tank, which is probably left over from the prior years } of running god know what through the lines. This "solution" is } currently running in several SEMs and microprobes, using a variety of } chillers (mostly Haskris which I have found to be much more reliable } than Coolwell). } } Works for me anyway. } john } } At 07:58 AM 1/11/2008, a.d.mckinnon-at-abdn.ac.uk wrote: } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Hi, } } } } We incorporate Sigma water bath treatment in the reservoir - 3.5ml per } } tank full (25L). } } } } Seems to work well - lasting about 12 months and got this recommendation } } } } from Sigma Technical Services re using it in chiller circulators for } } } EMs. } } } } TechServ_Number: S5525 } } TechServ_Name: SigmaClean; water bath treatment } } TechServ_Brand: SIGMA } } } } TechServ_re: is this product recommended for use in chiller circulators } } for } } the supply of cooling water for a transmission EM (Philips CM10). } } } } Best regards, } } } } Alastair } } } } Alastair McKinnon } } Histology & EM Facility Manager, IMS R2.62 } } University of Aberdeen, Institute of Medical Science } } Foresterhill, Aberdeen, AB25 2ZD } } tel: +44(0)1224 552923 (office); +44(0)1224 555911 (lab) } } fax: +44(0)1224 559533; email: a.d.mckinnon-at-abdn.ac.uk } } www.abdn.ac.uk/ims/h-em } } -----Original Message----- } } X-from: gary-at-gaugler.com [mailto:gary-at-gaugler.com] } } Sent: 10 January 2008 23:00 } } To: Mckinnon, Alastair D. } } Subject: [Microscopy] Re: Product for watercooling } } } } } } } } } } ------------------------------------------------------------------------ } } ---- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } America To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ------------------------------------------------------------------------ } } ---- } } } } I would suggest draining the water and replacing with distilled water. } } The Cl in tap water could lead to corrosion of metal in contact with the } } water. A good additive IMO is Ethylene Glycol as a 10% mix. I use } } technical grade EG and add 1/2 gallon to the 4.5 gallons of distilled } } water for 5 gallons total in the Haskris R050 chiller reservoir. } } } } Sometimes this holds up for a year. } } } } gary g. } } } } } } } } At 04:52 AM 1/10/2008, you wrote: } } } } } } } } } } } } } ----------------------------------------------------------------------- } } } ----- The Microscopy ListServer -- CoSponsor: The Microscopy Society } } } of America To Subscribe/Unsubscribe -- } } } http://www.microscopy.com/MicroscopyListserver } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ----------------------------------------------------------------------- } } } ----- } } } } } } Hi! } } } During some works in a room next to our TEM, one worker opened a } } } canalisation of the watercooling system, emptying the water tank of the } } } } } } watercooling device. } } } I filled it again with normal tap water but I think I have to add some } } } product to avoid mold growth. } } } Could you recommend one such product and where I can order it, } } } especially those of you from Germany and Austria (we are located in } } } Vienna, Austria)? } } } Our microscope is a Tecnai G20 and the watercooling unit is a Zephy ZEM } } } } } 1000S. } } } } } Thank you in advance, } } } Stephane } } } } } ==============================Original } } Headers============================== } } 11, 20 -- From gary-at-gaugler.com Thu Jan 10 16:56:56 2008 11, 20 -- } } Received: from qsmtp4.mc.surewest.net (qsmtp.mc.surewest.net } } [66.60.130.145]) } } 11, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP } } id m0AMutZj015411 } } 11, 20 -- for {microscopy-at-microscopy.com} ; Thu, 10 Jan 2008 } } 16:56:56 -0600 } } 11, 20 -- Message-Id: {200801102256.m0AMutZj015411-at-ns.microscopy.com} } } 11, 20 -- Received: (qmail 7535 invoked from network); 10 Jan 2008 } } 14:56:57 -0800 11, 20 -- Received: by simscan 1.1.0 ppid: 7531, pid: } } 7533, t: 0.0916s } } 11, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 } } 11, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) } } 11, 20 -- by qsmtp4 with SMTP; 10 Jan 2008 14:56:57 -0800 } } 11, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 11, 20 -- } } Date: Thu, 10 Jan 2008 14:56:53 -0800 11, 20 -- To: nizets2-at-yahoo.com } } 11, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 11, 20 -- Subject: Re: } } [Microscopy] Product for watercooling 11, 20 -- Cc: MSA listserver } } {microscopy-at-microscopy.com} 11, 20 -- In-Reply-To: } } {200801101252.m0ACqLYm004944-at-ns.microscopy.com} } } 11, 20 -- References: {200801101252.m0ACqLYm004944-at-ns.microscopy.com} } } 11, 20 -- Mime-Version: 1.0 } } 11, 20 -- Content-Type: text/plain; charset=us-ascii; format=flowed; } } x-avg-checked=avg-ok-360B2309 ==============================End of - } } Headers============================== } } } } } } } } ==============================Original Headers============================== } } 25, 24 -- From a.d.mckinnon-at-abdn.ac.uk Fri Jan 11 09:47:45 2008 } } 25, 24 -- Received: from mailhub3.abdn.ac.uk (mailhub3.abdn.ac.uk } } [139.133.7.13]) } } 25, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } } ESMTP id m0BFlgKZ023642 } } 25, 24 -- for {Microscopy-at-microscopy.com} ; Fri, 11 Jan 2008 } } 09:47:44 -0600 } } 25, 24 -- Received: from ew-mail-b.uoa.abdn.ac.uk ([139.133.15.21] } } helo=VMAIL1.uoa.abdn.ac.uk) } } 25, 24 -- by mailhub3.abdn.ac.uk with esmtp (Exim 4.52) } } 25, 24 -- id 1JDM6b-00024t-4r; Fri, 11 Jan 2008 15:47:37 +0000 } } 25, 24 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 } } 25, 24 -- Content-class: urn:content-classes:message } } 25, 24 -- MIME-Version: 1.0 } } 25, 24 -- Content-Type: text/plain; } } 25, 24 -- charset="US-ASCII" } } 25, 24 -- Subject: RE: [Microscopy] Re: Product for watercooling } } 25, 24 -- Date: Fri, 11 Jan 2008 15:45:11 -0000 } } 25, 24 -- Message-ID: } } {009B2FCFB3DD25408A4BBC240F091376BD20A5-at-VMAIL1.uoa.abdn.ac.uk} } } 25, 24 -- X-MS-Has-Attach: } } 25, 24 -- X-MS-TNEF-Correlator: } } 25, 24 -- Thread-Topic: [Microscopy] Re: Product for watercooling } } 25, 24 -- thread-index: AchT3EvpoZa+Q4/RS2y35Xsyfvhq1gAi/Eww } } 25, 24 -- From: "Mckinnon, Alastair D." {a.d.mckinnon-at-abdn.ac.uk} } } 25, 24 -- To: {gary-at-gaugler.com} } } 25, 24 -- Cc: {Microscopy-at-microscopy.com} } } 25, 24 -- Content-Transfer-Encoding: 8bit } } 25, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } } ns.microscopy.com id m0BFlgKZ023642 } } ==============================End of - Headers============================== } } } } } ==============================Original Headers============================== } 8, 21 -- From donovan-at-uoregon.edu Fri Jan 11 10:37:25 2008 } 8, 21 -- Received: from smtp.uoregon.edu (mserv1.uoregon.edu [128.223.142.40]) } 8, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0BGbOfe005078 } 8, 21 -- for {microscopy-at-microscopy.com} ; Fri, 11 Jan 2008 10:37:24 -0600 } 8, 21 -- Received: from Probev.uoregon.edu (probev.uoregon.edu [128.223.92.75]) } 8, 21 -- (authenticated bits=0) } 8, 21 -- by smtp.uoregon.edu (8.13.8/8.13.8) with ESMTP id m0BGbNgM019167 } 8, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) } 8, 21 -- for {microscopy-at-microscopy.com} ; Fri, 11 Jan 2008 08:37:23 -0800 } 8, 21 -- Message-Id: {200801111637.m0BGbNgM019167-at-smtp.uoregon.edu} } 8, 21 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 } 8, 21 -- Date: Fri, 11 Jan 2008 08:37:13 -0800 } 8, 21 -- To: microscopy-at-microscopy.com } 8, 21 -- From: John Donovan {donovan-at-uoregon.edu} } 8, 21 -- Subject: Re: [Microscopy] Product for watercooling } 8, 21 -- In-Reply-To: {200801111558.m0BFwJHR032212-at-ns.microscopy.com} } 8, 21 -- References: {200801111558.m0BFwJHR032212-at-ns.microscopy.com} } 8, 21 -- Mime-Version: 1.0 } 8, 21 -- Content-Type: text/plain; charset="us-ascii"; format=flowed } 8, 21 -- X-Virus-Scanned: ClamAV 0.92/5478/Fri Jan 11 07:39:22 2008 on mserv1 } 8, 21 -- X-Virus-Status: Clean } ==============================End of - Headers============================== } }
-- ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- Bryan Bandli Senior Research Scientist MVA Scientific Consultants 3300 Breckinridge Blvd., Suite 400 (770) 662-8509 bbandli-at-mvainc.com www.mvainc.com ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- The information in this email is confidential and may be legally privileged. It is intended solely for the addressee. If you are not the intended recipient, please delete the email and notify MVA Scientific Consultants of the transmission error. MVA Scientific Consultants - 3300 Breckinridge Blvd. Suite 400, Duluth, GA 30096 - (770)662-8509 -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
==============================Original Headers============================== 5, 20 -- From bbandli-at-mvainc.com Fri Jan 11 10:56:17 2008 5, 20 -- Received: from smtp03.atlngahp.sys.nuvox.net (smtp-out3.atlngahp.sys.nuvox.net [70.43.63.20]) 5, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0BGuHYF017808 5, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 11 Jan 2008 10:56:17 -0600 5, 20 -- Received: from [192.168.1.95] (216.215.228.34.nw.nuvox.net [216.215.228.34]) 5, 20 -- by smtp03.atlngahp.sys.nuvox.net (8.13.1/8.13.1) with ESMTP id m0BGuCXC012385 5, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 11 Jan 2008 11:56:12 -0500 5, 20 -- Message-ID: {4787A036.9030005-at-mvainc.com} 5, 20 -- Date: Fri, 11 Jan 2008 11:58:30 -0500 5, 20 -- From: bbandli {bbandli-at-mvainc.com} 5, 20 -- Reply-To: bbandli-at-mvainc.com 5, 20 -- Organization: MVA Scientific Consultants 5, 20 -- User-Agent: Thunderbird 2.0.0.9 (Windows/20071031) 5, 20 -- MIME-Version: 1.0 5, 20 -- To: Microscopy-at-microscopy.com 5, 20 -- Subject: Re: Product for watercooling 5, 20 -- References: {200801111637.m0BGbreR006045-at-ns.microscopy.com} 5, 20 -- In-Reply-To: {200801111637.m0BGbreR006045-at-ns.microscopy.com} 5, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 20 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both modla-at-dbi.udel.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: modla-at-dbi.udel.edu Name: Shannon Modla
Organization: University of Delaware
Title-Subject: [Filtered] TEM Recommendations
Question: I work in a multi-user bio-imaging facility, and we recently were funded to purchase a new TEM. We are interested in a TEM that is EM tomography capable with a high resolution CCD camera and is user-friendly. We plan to upgrade to include a cryo-stage in the near future, so an instrument that is cryo-ready would be beneficial.
I was wondering if anyone could provide me with recommendations for TEMs and their experience with tomography. Also, is there a good test sample that can be used to test EM tomography both with cryo and conventional preparations for the demos?
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Email: mckee-at-helix.mgh.harvard.edu Name: Mary McKee
Organization: MGH
Title-Subject: [Filtered] service for older Reichert Ultrqacut E ultramicrotomes
Question: We have 2 older (not ancient) working Ultracut E ultramicrotomes that we would like to have routine service on (cleaning, lubricating, etc). Leica will not service them any longer. Does anyone know of an independent person for the northeast region (Boston)? Thanks in advance.
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Email: alissa.susanne-at-gmail.com Name: Alissa H
Organization: University of Maryland
Title-Subject: [Filtered] specimen preparation
Question: We have a new Zeiss AxioImager here, and I've been given the task of not only learning how to use it, but also one of my main projects involves use of the microscope to evaluate fluorescence of some bacteria. I've transformed a plasmid with YFP on it into some streptococcus. I can find the buggers, take a reasonable Z stack, and even deconvolve the image.
What I'd like to learn is better ways to prepare my samples, right now I've just been making wet mounts with saline. I'd also like to learn how to better use Zeiss' computer program that we got with the microscope. I don't find the program intuitive, like I do for most computer programs, and am getting frustrated. If anyone can give me good suggestions on where I can read about these things, I'd really appreciate it. I feel like a total newbie, and I'm not quite sure where to go to get good information.
On Jan 11, 2008, at 3:30 PM, modla-at-dbi.udel.edu wrote:
} I work in a multi-user bio-imaging facility, and we recently were } funded to purchase a new TEM. We are interested in a TEM that is } EM tomography capable with a high resolution CCD camera and is user- } friendly. We plan to upgrade to include a cryo-stage in the near } future, so an instrument that is cryo-ready would be beneficial. } } } } I was wondering if anyone could provide me with recommendations for } TEMs and their experience with tomography. Also, is there a good } test sample that can be used to test EM tomography both with cryo } and conventional preparations for the demos? } Dear Shannon, To a great extent, the type of instrument necessary depends on the specimens for which you wish to do tomography. If, for example, you want to look at bacterial cells, then you will need a higher voltage than if you only wish to look at viruses or protein complexes. Our lab has had a very good experience with FEI instruments. The Tecnai T12, which, while not our primary tomographic instrument, has both tomographic and cryo capabilities, is very reliable and user friendly. It is an off-the-shelf, but very high-end TEM. It is limited, however, to specimens thinner than a typical bacterium. Our main instrument is a Tecnai TF30H Polara with all the bells and whistles, most of which are necessary for electron cryo-tomography, especially of whole bacterial cells. It has a field-emission source, necessary for good beam coherence for phase contrast; it operates at 300 kV, which provides the penetrating power to observe ~0.5 um specimens tilted to 70 degrees (an effective thickness of 1.5 um); its cartridge system for holding specimens provides accurate and reproducible tilting for good tracking during tilt-series data collection; it has an energy filter, which provides increased contrast and resolution by looking only at elastically scattered electrons; and, finally, it is equipped with a 4k x 4k GATAN Ultracam. It too is pretty reliable, but, being more complicated than the T12, there are more things that can go wrong. The only drawback to the Polara is its price, but I would hope that any administrator who wants to get into the electron cryo-tomography field would be willing to spend what is necessary. I would suggest using a 250 nm conventional plastic section for a room temperature test object and whatever is your favorite specimen--limited to a thickness that suits the instrument--for a cryo test object. Yours, Bill Tivol, PhD EM Scientist Electron Cryo-Microscopy Facility Broad Center, Mail Code 114-96 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 4, 23 -- From tivol-at-caltech.edu Fri Jan 11 18:56:09 2008 4, 23 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 4, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0C0u9oY017616 4, 23 -- for {microscopy-at-msa.microscopy.com} ; Fri, 11 Jan 2008 18:56:09 -0600 4, 23 -- Received: from fire-dog.its.caltech.edu (fire-dog [192.168.1.4]) 4, 23 -- by wood-ox-postvirus (Postfix) with ESMTP id D31AA13E03; 4, 23 -- Fri, 11 Jan 2008 16:56:07 -0800 (PST) 4, 23 -- Received: from [192.168.159.158] (jpix-01.caltech.edu [131.215.2.133]) 4, 23 -- by fire-ox.its.caltech.edu (Postfix) with ESMTP id 78C7213E6F; 4, 23 -- Fri, 11 Jan 2008 16:56:01 -0800 (PST) 4, 23 -- In-Reply-To: {200801112330.m0BNUpBP010814-at-ns.microscopy.com} 4, 23 -- References: {200801112330.m0BNUpBP010814-at-ns.microscopy.com} 4, 23 -- Mime-Version: 1.0 (Apple Message framework v753) 4, 23 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 4, 23 -- Message-Id: {7C65E385-CA51-4A97-A54D-ABBD831BD3C9-at-caltech.edu} 4, 23 -- Cc: microscopy-at-msa.microscopy.com 4, 23 -- Content-Transfer-Encoding: 7bit 4, 23 -- From: Bill Tivol {tivol-at-caltech.edu} 4, 23 -- Subject: Re: [Microscopy] viaWWW: TEM Recommendations 4, 23 -- Date: Fri, 11 Jan 2008 16:55:59 -0800 4, 23 -- To: modla-at-dbi.udel.edu 4, 23 -- X-Mailer: Apple Mail (2.753) 4, 23 -- X-Spam-Scanned: at Caltech-ITS on fire-dog by amavisd-2.4.5 ==============================End of - Headers==============================
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (r.kirk-at-kingston.ac.uk) from http://microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Sunday, January 13, 2008 at 07:07:47 ---------------------------------------------------------------------------
Email: r.kirk-at-kingston.ac.uk Name: Dr Ruth Kirk
Organization: Kingston University
Education: Undergraduate College
Location: Surrey, UK
Question: I am currently supervising a student project which will investigate the use of different types of preparation techniques for SEM of protists. Do you have any advice on aggregating the protists during processing? That is, can you stick them to a cover slip or similar?
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.org/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both panxijiang-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: panxijiang-at-gmail.com Name: Xijiang Pan
Organization: Tsinghua University
Title-Subject: [Filtered] how to perform "bake out" procedure on the FEI CM series TEM
Question: I am wondering if there is anybody in this list farmiliar with the "bake out" procedure for the FEI CM serious TEM. The local service engineers do not have any experiences in such work. So if you know,please contact me. Thanks in advance.
Just out of curiosity, since a chiller is a relatively closed system, what about putting a dilute chlorine solution in, like that used in pools? Or perhaps a slight bleach additive? Would that damage the equipment?
--Justin A. Kraft
On Jan 11, 2008 12:05 PM, {bbandli-at-mvainc.com} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I second John's "solution". It's what we have been using for our FESEM } for several years without any problems. Opaque lines from the chiller } to the scope are essential however. Even though this is what this scope } has been on since day 1, we do have a small amount of rust/grit in the } bottom of the tank but it hasn't been a problem. We also change the H2O } every 6months (Per JEOL service). } Cheers, } Bryan Bandli } } donovan-at-uoregon.edu wrote: } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } All, } } After much fighting of corrosion and algae issues I settled years ago } } on what might be the easiest and most long term "solution". That is } } pure distilled water. And here's the secret, you don't need to add } } any chemical additives if you remove all clear plastic hoses from the } } system and replace them with black or otherwise opaque materials. No } } light, no growth. } } } } With this "solution" I have found I can run for years with a crystal } } clear tank and with only an occasional speck of rust visible in the } } bottom of the tank, which is probably left over from the prior years } } of running god know what through the lines. This "solution" is } } currently running in several SEMs and microprobes, using a variety of } } chillers (mostly Haskris which I have found to be much more reliable } } than Coolwell). } } } } Works for me anyway. } } john } } } } At 07:58 AM 1/11/2008, a.d.mckinnon-at-abdn.ac.uk wrote: } } } } } } } } } } } ---------------------------------------------------------------------------- } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ---------------------------------------------------------------------------- } } } } } } Hi, } } } } } } We incorporate Sigma water bath treatment in the reservoir - 3.5ml per } } } tank full (25L). } } } } } } Seems to work well - lasting about 12 months and got this recommendation } } } } } } from Sigma Technical Services re using it in chiller circulators for } } } } } EMs. } } } } } } TechServ_Number: S5525 } } } TechServ_Name: SigmaClean; water bath treatment } } } TechServ_Brand: SIGMA } } } } } } TechServ_re: is this product recommended for use in chiller circulators } } } for } } } the supply of cooling water for a transmission EM (Philips CM10). } } } } } } Best regards, } } } } } } Alastair } } } } } } Alastair McKinnon } } } Histology & EM Facility Manager, IMS R2.62 } } } University of Aberdeen, Institute of Medical Science } } } Foresterhill, Aberdeen, AB25 2ZD } } } tel: +44(0)1224 552923 (office); +44(0)1224 555911 (lab) } } } fax: +44(0)1224 559533; email: a.d.mckinnon-at-abdn.ac.uk } } } www.abdn.ac.uk/ims/h-em } } } -----Original Message----- } } } X-from: gary-at-gaugler.com [mailto:gary-at-gaugler.com] } } } Sent: 10 January 2008 23:00 } } } To: Mckinnon, Alastair D. } } } Subject: [Microscopy] Re: Product for watercooling } } } } } } } } } } } } } } } ------------------------------------------------------------------------ } } } ---- } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } } America To Subscribe/Unsubscribe -- } } } http://www.microscopy.com/MicroscopyListserver } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ------------------------------------------------------------------------ } } } ---- } } } } } } I would suggest draining the water and replacing with distilled water. } } } The Cl in tap water could lead to corrosion of metal in contact with the } } } water. A good additive IMO is Ethylene Glycol as a 10% mix. I use } } } technical grade EG and add 1/2 gallon to the 4.5 gallons of distilled } } } water for 5 gallons total in the Haskris R050 chiller reservoir. } } } } } } Sometimes this holds up for a year. } } } } } } gary g. } } } } } } } } } } } } At 04:52 AM 1/10/2008, you wrote: } } } } } } } } } } } } } } } } } } } ----------------------------------------------------------------------- } } } } ----- The Microscopy ListServer -- CoSponsor: The Microscopy Society } } } } of America To Subscribe/Unsubscribe -- } } } } http://www.microscopy.com/MicroscopyListserver } } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } } ----------------------------------------------------------------------- } } } } ----- } } } } } } } } Hi! } } } } During some works in a room next to our TEM, one worker opened a } } } } canalisation of the watercooling system, emptying the water tank of the } } } } } } } } watercooling device. } } } } I filled it again with normal tap water but I think I have to add some } } } } product to avoid mold growth. } } } } Could you recommend one such product and where I can order it, } } } } especially those of you from Germany and Austria (we are located in } } } } Vienna, Austria)? } } } } Our microscope is a Tecnai G20 and the watercooling unit is a Zephy ZEM } } } } } } } 1000S. } } } } } } } Thank you in advance, } } } } Stephane } } } } } } } ==============================Original } } } Headers============================== } } } 11, 20 -- From gary-at-gaugler.com Thu Jan 10 16:56:56 2008 11, 20 -- } } } Received: from qsmtp4.mc.surewest.net (qsmtp.mc.surewest.net } } } [66.60.130.145]) } } } 11, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP } } } id m0AMutZj015411 } } } 11, 20 -- for {microscopy-at-microscopy.com} ; 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} } } 25, 24 -- charset="US-ASCII" } } } 25, 24 -- Subject: RE: [Microscopy] Re: Product for watercooling } } } 25, 24 -- Date: Fri, 11 Jan 2008 15:45:11 -0000 } } } 25, 24 -- Message-ID: } } } {009B2FCFB3DD25408A4BBC240F091376BD20A5-at-VMAIL1.uoa.abdn.ac.uk} } } } 25, 24 -- X-MS-Has-Attach: } } } 25, 24 -- X-MS-TNEF-Correlator: } } } 25, 24 -- Thread-Topic: [Microscopy] Re: Product for watercooling } } } 25, 24 -- thread-index: AchT3EvpoZa+Q4/RS2y35Xsyfvhq1gAi/Eww } } } 25, 24 -- From: "Mckinnon, Alastair D." {a.d.mckinnon-at-abdn.ac.uk} } } } 25, 24 -- To: {gary-at-gaugler.com} } } } 25, 24 -- Cc: {Microscopy-at-microscopy.com} } } } 25, 24 -- Content-Transfer-Encoding: 8bit } } } 25, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } } } ns.microscopy.com id m0BFlgKZ023642 } } } ==============================End of - Headers============================== } } } } } } } } } ==============================Original Headers============================== } } 8, 21 -- From donovan-at-uoregon.edu Fri Jan 11 10:37:25 2008 } } 8, 21 -- Received: from smtp.uoregon.edu (mserv1.uoregon.edu [128.223.142.40]) } } 8, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0BGbOfe005078 } } 8, 21 -- for {microscopy-at-microscopy.com} ; 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If you are not the intended recipient, please delete the email and notify MVA Scientific Consultants of the transmission error. } MVA Scientific Consultants - 3300 Breckinridge Blvd. Suite 400, Duluth, GA 30096 - (770)662-8509 } ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- } } } } } ==============================Original Headers============================== } 5, 20 -- From bbandli-at-mvainc.com Fri Jan 11 10:56:17 2008 } 5, 20 -- Received: from smtp03.atlngahp.sys.nuvox.net (smtp-out3.atlngahp.sys.nuvox.net [70.43.63.20]) } 5, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0BGuHYF017808 } 5, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 11 Jan 2008 10:56:17 -0600 } 5, 20 -- Received: from [192.168.1.95] (216.215.228.34.nw.nuvox.net [216.215.228.34]) } 5, 20 -- by smtp03.atlngahp.sys.nuvox.net (8.13.1/8.13.1) with ESMTP id m0BGuCXC012385 } 5, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 11 Jan 2008 11:56:12 -0500 } 5, 20 -- Message-ID: {4787A036.9030005-at-mvainc.com} } 5, 20 -- Date: Fri, 11 Jan 2008 11:58:30 -0500 } 5, 20 -- From: bbandli {bbandli-at-mvainc.com} } 5, 20 -- Reply-To: bbandli-at-mvainc.com } 5, 20 -- Organization: MVA Scientific Consultants } 5, 20 -- User-Agent: Thunderbird 2.0.0.9 (Windows/20071031) } 5, 20 -- MIME-Version: 1.0 } 5, 20 -- To: Microscopy-at-microscopy.com } 5, 20 -- Subject: Re: Product for watercooling } 5, 20 -- References: {200801111637.m0BGbreR006045-at-ns.microscopy.com} } 5, 20 -- In-Reply-To: {200801111637.m0BGbreR006045-at-ns.microscopy.com} } 5, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 5, 20 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers============================== }
==============================Original Headers============================== 3, 29 -- From kraftpiano-at-gmail.com Sun Jan 13 12:49:48 2008 3, 29 -- Received: from rv-out-0910.google.com (rv-out-0910.google.com [209.85.198.189]) 3, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0DInlkf028510 3, 29 -- for {microscopy-at-microscopy.com} ; Sun, 13 Jan 2008 12:49:47 -0600 3, 29 -- Received: by rv-out-0910.google.com with SMTP id k20so1660524rvb.30 3, 29 -- for {microscopy-at-microscopy.com} ; Sun, 13 Jan 2008 10:49:46 -0800 (PST) 3, 29 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 3, 29 -- d=gmail.com; s=gamma; 3, 29 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:in-reply-to:mime-version:content-type:content-transfer-encoding:content-disposition:references; 3, 29 -- bh=bcp/zJX1peW1n8x2DKC4//HpaWYEo1lQK1S7slKox20=; 3, 29 -- b=SuLr1tQOXEU4RLmIJZj4fnKOALp7Kvqz7QSYrVZdzNdwpa54uEQf/Kjw/R5nf+89isn8wPUMxugW5a12zniVeb/qPjm6oBg1WrAG/Bh7zyv/MUgvHXxk72CsIrDbQTLAAJY053LF/KKKyVk9vr5ho+TcZFqeLYq/VdlIsHqrkuo= 3, 29 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 3, 29 -- d=gmail.com; s=gamma; 3, 29 -- h=message-id:date:from:to:subject:in-reply-to:mime-version:content-type:content-transfer-encoding:content-disposition:references; 3, 29 -- b=AoftEWdLtldcc19ecfiF3e5n7oXQv2FJtSN6La1Ek79iD8crC9bh0geNCN1+KpHj2zvebFB+VScxzHfoFaU3rfisJHVq0dQaziUKWfdXG1YKOhZuAHIjzeCs+7wqtqTzHJi+VwwRnhPH4376xoKqcKeZNdKl1NpRrT8tcV9Ya0k= 3, 29 -- Received: by 10.141.202.12 with SMTP id e12mr3312543rvq.91.1200250186517; 3, 29 -- Sun, 13 Jan 2008 10:49:46 -0800 (PST) 3, 29 -- Received: by 10.140.161.11 with HTTP; Sun, 13 Jan 2008 10:49:46 -0800 (PST) 3, 29 -- Message-ID: {25e2b0d20801131049i7ed2c6c5nc208fec10dbd475b-at-mail.gmail.com} 3, 29 -- Date: Sun, 13 Jan 2008 13:49:46 -0500 3, 29 -- From: "Justin Kraft" {kraftpiano-at-gmail.com} 3, 29 -- To: bbandli-at-mvainc.com, microscopy-at-microscopy.com 3, 29 -- Subject: Re: [Microscopy] Re: Product for watercooling 3, 29 -- In-Reply-To: {200801111705.m0BH5RW6027968-at-ns.microscopy.com} 3, 29 -- MIME-Version: 1.0 3, 29 -- Content-Type: text/plain; charset=ISO-8859-1 3, 29 -- Content-Transfer-Encoding: 7bit 3, 29 -- Content-Disposition: inline 3, 29 -- References: {200801111705.m0BH5RW6027968-at-ns.microscopy.com} ==============================End of - Headers==============================
While chlorine bleach will kill most stuff, at least one chiller manufacturer (Haskris) says that more than ~7ppm free Cl will damage the buna-N O-ring seals. I worked it out a few years ago and figured that it was ~50ml of household bleach in our chiller tank.
We have occasionally done a shock treatment with a high concentration of bleach to kill stuff in the tank, then flushed it out. We've not had any trouble with the seals so far. Minimizing the amount of light that gets to the water makes a real difference in the amount of gunk that develops.
Cheers, Henk
At 01:51 PM 1/13/2008, kraftpiano-at-gmail.com wrote:
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Hendrik O. Colijn colijn.1-at-osu.edu OSU Campus Electron Optics Facility (614) 292-0674 040 Fontana Labs, 116 W. 19th Ave www.ceof.ohio-state.edu
==============================Original Headers============================== 11, 26 -- From colijn.1-at-osu.edu Sun Jan 13 14:04:17 2008 11, 26 -- Received: from er6s1.ecr6.ohio-state.edu (er6s1.ecr6.ohio-state.edu [164.107.76.2]) 11, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0DK4Hx2010488 11, 26 -- for {microscopy-at-microscopy.com} ; Sun, 13 Jan 2008 14:04:17 -0600 11, 26 -- Received: from CONVERSION-DAEMON.er6s1.ecr6.ohio-state.edu by 11, 26 -- er6s1.ecr6.ohio-state.edu (PMDF V6.3-x13 #31056) 11, 26 -- id {01MQ1RHJAGTSACU47A-at-ecr6.ohio-state.edu} for microscopy-at-microscopy.com; 11, 26 -- Sun, 13 Jan 2008 15:04:16 -0500 (EST) 11, 26 -- Received: from HOC3.osu.edu 11, 26 -- (d118-75-136-133.try.wideopenwest.com [75.118.133.136]) 11, 26 -- by er6s1.ecr6.ohio-state.edu (PMDF V6.3-x13 #31056) 11, 26 -- with ESMTPA id {01MQ1RHI06OUACH3MM-at-ecr6.ohio-state.edu} ; Sun, 11, 26 -- 13 Jan 2008 15:04:16 -0500 (EST) 11, 26 -- Date: Sun, 13 Jan 2008 15:01:41 -0500 11, 26 -- From: "Hendrik O. Colijn" {colijn.1-at-osu.edu} 11, 26 -- Subject: Re: [Microscopy] Product for watercooling 11, 26 -- In-reply-to: {200801131851.m0DIpgXQ030978-at-ns.microscopy.com} 11, 26 -- Sender: colijn-at-er6s1.ecr6.ohio-state.edu 11, 26 -- To: kraftpiano-at-gmail.com 11, 26 -- Cc: microscopy-at-microscopy.com 11, 26 -- Message-id: {7.0.1.0.2.20080113144228.027d52c8-at-osu.edu} 11, 26 -- MIME-version: 1.0 11, 26 -- X-Mailer: QUALCOMM Windows Eudora Version 7.0.1.0 11, 26 -- Content-type: text/plain; charset=us-ascii; format=flowed 11, 26 -- X-Env-From: auth/colijn.1-at-osu.edu 11, 26 -- References: {200801131851.m0DIpgXQ030978-at-ns.microscopy.com} ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both PRICE-at-gw.med.sc.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: PRICE-at-gw.med.sc.edu Name: Bob Price
Organization: USC- School of Medicine
Title-Subject: [Filtered] Basic Confocal Microscopy and Digital Imaging Workshop
Question: Dear List Members,
Appended below is information about our fourth Basic Confocal Microscopy and Digital Imaging Workshop that will be held at the University of South Carolina School of Medicine from June 16-20, 2008.
Workshop on Basic Confocal Microscopy and Digital Imaging: Brief Synopsis
The South Carolina EPSCoR/IDeA Program and the USC School of Medicine Instrumentation Resource Facility are pleased to announce the 4th annual workshop on Basic Confocal Microscopy and Digital Imaging. The hands-on workshop will target beginning and intermediate users of confocal microscopes and will provide lectures from experts in the field of confocal microscopy and the use of Adobe Photoshop and 3-D software for processing of confocal images. Lecture material will provide information on the basics of fluorescence and fluorescent probes, biological specimen preparation (fixation, staining, optical properties and mounting materials), strategies and protocols for selection of antibody labeling, the basic components of a confocal microscope (lasers, dichroic mirrors, microscope objectives, photomultiplier tubes, etc.) and an overview of some applications of confocal microscopy.
During the laboratory portion of the workshop specimens will be processed for double and triple labeling and proper selection of user adjustable parameters to optimize image collection will be addressed and demonstrated. Participants are welcome to process their own samples or to use samples that will be provided. Several point scanning and spinning disk confocal systems from various manufacturers will be available for use so participants will have ample time for hands on use of the instruments during the workshop.
Faculty scheduled to participate include:
Dr. Bob Price, Research Professor, Cell and Developmental Biology and Anatomy, and Director, Instrumentation Resource Facility, University of South Carolina School of Medicine (http://dba.med.sc.edu/price/irf/irf.htm) Dr. Jay Jerome, Associate Professor, Pathology and Cancer Biology, Vanderbilt University (https://medschool.mc.vanderbilt.edu/facultydata/php_files/show_faculty.php?id3=1032) Dr. Ralph Albrecht, Professor, Department of Animal Sciences, University of Wisconsin-Madison (http://www.ansci.wisc.edu/facstaff/Faculty/pages/albrecht/index.htm) Dr. John Mackenzie, Professor, Microbiology, North Carolina State University (http://www.ncsu.edu/cem/index.html) Dr. Tom Trusk, Associate Professor, Department of Cell Biology and Anatomy, Medical University of South Carolina (http://cba.musc.edu/faculty/TruskT.htm)
Past workshops have been very successful with over 50 attendees at each. For further information contact Bob Price (Price-at-med.sc.edu) or visit the workshop website: http://dba.med.sc.edu/price/irf/irf.htm
Robert L. Price, PhD Research Professor and Director, Instrumentation Resource Facility School of Medicine, USC Bldg 1 Room B60 6439 Garner's Ferry Road Columbia, SC 29208
Perhaps it would comfort you to know that I have exactly the same feeling with our Zeiss axiovert. The microscope itself is well designed but the software is user-unfriendly. The main operations, those obligatory steps that you use all the time are dispersed in different commands and subcommands. I constantly feel that I am using only 0,1% of the possibilities of the system, without hope of increasing this percentage. The best answer I can give you is to arrange a course with Zeiss.
Best regards, Stephane
PS: Z-stack on bacteria with a light microscope?? Are you sure you are not talking about confocal?
----- Original Message ---- X-from: "alissa.susanne-at-gmail.com" {alissa.susanne-at-gmail.com} To: nizets2-at-yahoo.com Sent: Saturday, January 12, 2008 12:38:35 AM
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both alissa.susanne-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: alissa.susanne-at-gmail.com Name: Alissa H
Organization: University of Maryland
Title-Subject: [Filtered] specimen preparation
Question: We have a new Zeiss AxioImager here, and I've been given the task of not only learning how to use it, but also one of my main projects involves use of the microscope to evaluate fluorescence of some bacteria. I've transformed a plasmid with YFP on it into some streptococcus. I can find the buggers, take a reasonable Z stack, and even deconvolve the image.
What I'd like to learn is better ways to prepare my samples, right now I've just been making wet mounts with saline. I'd also like to learn how to better use Zeiss' computer program that we got with the microscope. I don't find the program intuitive, like I do for most computer programs, and am getting frustrated. If anyone can give me good suggestions on where I can read about these things, I'd really appreciate it. I feel like a total newbie, and I'm not quite sure where to go to get good information.
==============================Original Headers============================== 8, 11 -- From zaluzec-at-microscopy.com Fri Jan 11 17:31:25 2008 8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0BNVO20012072 8, 11 -- for {microscopy-at-microscopy.com} ; Fri, 11 Jan 2008 17:31:25 -0600 8, 11 -- Mime-Version: 1.0 8, 11 -- Message-Id: {p06240803c3adacbe9638-at-[206.69.208.22]} 8, 11 -- Date: Fri, 11 Jan 2008 17:31:23 -0600 8, 11 -- To: microscopy-at-microscopy.com 8, 11 -- From: alissa.susanne-at-gmail.com (by way of MicroscopyListserver) 8, 11 -- Subject: viaWWW: specimen preparation 8, 11 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers==============================
____________________________________________________________________________________ Never miss a thing. Make Yahoo your home page. http://www.yahoo.com/r/hs
==============================Original Headers============================== 20, 20 -- From nizets2-at-yahoo.com Mon Jan 14 08:11:50 2008 20, 20 -- Received: from web37413.mail.mud.yahoo.com (web37413.mail.mud.yahoo.com [209.191.91.145]) 20, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m0EEBoZ5002890 20, 20 -- for {microscopy-at-microscopy.com} ; Mon, 14 Jan 2008 08:11:50 -0600 20, 20 -- Received: (qmail 52176 invoked by uid 60001); 14 Jan 2008 14:11:49 -0000 20, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 20, 20 -- s=s1024; d=yahoo.com; 20, 20 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:Cc:MIME-Version:Content-Type:Message-ID; 20, 20 -- b=6FI8gNzwtHWOpPeRmsrj+Kr2cwszebzN6qHTZJgP2U9qV0/6wLXogGBSkFxpes+oCYSNMRY8dnCrcgb2ILcPSDGu7wdBcko+hYb0NJY3WmfJvAeBMFinINIaEdQ2LMmtIshVXAzR6mCKXABFVVIeWRZIjb5kDfEWDpRvXd0d/VA=; 20, 20 -- X-YMail-OSG: qaADhCQVM1mMhSXYkNxinWbgAKMDmxo6WBuogdroqI.c7TkpIZoGpeIlVKFiEMVnWjjGs4x12FVdu_6LqTuaN_XGJG9IjdNDAgjXDnWa.QyzpMBKD8JExrg8uWlltpR1OkAqqnc3XAuqSyyeGc5PIKkIuRETqzD8Hfas.Q60lHW0 20, 20 -- Received: from [80.122.101.100] by web37413.mail.mud.yahoo.com via HTTP; Mon, 14 Jan 2008 06:11:49 PST 20, 20 -- X-Mailer: YahooMailRC/818.31 YahooMailWebService/0.7.160 20, 20 -- Date: Mon, 14 Jan 2008 06:11:49 -0800 (PST) 20, 20 -- From: Stephane Nizet {nizets2-at-yahoo.com} 20, 20 -- Subject: Re: [Microscopy] viaWWW: specimen preparation 20, 20 -- To: alissa.susanne-at-gmail.com 20, 20 -- Cc: microscopy-at-microscopy.com 20, 20 -- MIME-Version: 1.0 20, 20 -- Content-Type: text/plain; charset=us-ascii 20, 20 -- Message-ID: {771437.52040.qm-at-web37413.mail.mud.yahoo.com} ==============================End of - Headers==============================
I agree about the opaque lines but found it much easier to use normal transparent hoses and wrap them in aluminium foil with a bit of tape. It's worked for years on our SEM.
Malcolm
Malcolm Haswell e.m. unit Chemispec School of Health, Natural and Social Sciences Fleming Building University of Sunderland Tyne & Wear SR1 3SD UK e-mail: malcolm.haswell-at-sunderland.ac.uk
----- Original Message ----- X-from: bbandli-at-mvainc.com
Preventing the growth of algae in cooling water systems is discussed in detail in my book "Vacuum Methods in Electron Microscopy" Two chemicals commonly used to prevent algal growth in such systems are Chloramine T and dichlorophene. Both of these chemicals can be obtained from various specialty chemical companies. You can also probably obtain algaecides from companies (and merchants) that sell water beds, and swimming pool equipment. I do not recommend the use of ethylene glycol for several reasons that are discussed in my book. Also, remember that algae require light in order to grow, and so you can substantially inhibit their growth by fully excluding light from the system (i.e. cover the reservoir with a a light-tight cover, and use fully opaque tubing). Changing from ordinary tap water to distilled water will probably not give you much of an advantage, except for possibly minimizing the formation of a bit of scale in the heated parts of the diffusion pump. However, the amount of scale formation should be quite limited since it is a closed system containing a limited amount of water. -- Wilbur C. Bigelow, Professor Emeritus Materials Sci. & Engr., Univ. of Michigan Ann Arbor, Michigan 48109-2136 e-mail: bigelow-at-umich.edu; Fx:734-763-4788; Ph:734-975-0858 Address mail to: 2911 Whittier Court Ann Arbor, MI 48104-6731
==============================Original Headers============================== 1, 14 -- From bigelow-at-umich.edu Mon Jan 14 15:19:40 2008 1, 14 -- Received: from skycaptain.mr.itd.umich.edu (smtp.mail.umich.edu [141.211.93.160]) 1, 14 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0ELJed0012094 1, 14 -- for {microscopy-at-microscopy.com} ; Mon, 14 Jan 2008 15:19:40 -0600 1, 14 -- Received: FROM [141.212.131.221] (bigelow-g4.engin.umich.edu [141.212.131.221]) 1, 14 -- BY skycaptain.mr.itd.umich.edu ID 478BD1EA.336C2.17443 ; 1, 14 -- 14 Jan 2008 16:19:38 -0500 1, 14 -- Mime-Version: 1.0 1, 14 -- Message-Id: {p06240800c3b17f44c5a5-at-[141.212.131.221]} 1, 14 -- Date: Mon, 14 Jan 2008 16:19:34 -0500 1, 14 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 1, 14 -- From: Wil Bigelow {bigelow-at-umich.edu} 1, 14 -- Subject: [Microscopy] RE: Algaecides 1, 14 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both slc6-at-lehigh.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: slc6-at-lehigh.edu Name: Sharon Coe
Organization: Lehigh Microscopy School
Title-Subject: [Filtered] Lehigh Microscopy School Courses
Question: There is still time to register for the 2008 Lehigh Microscopy School which will be held June 1-13, 2008. This will be the 38th year of course offerings which include:
SEM and X-Ray Microanalaysis (June 2-6)
Introduction to SEM and EDS for the New Operator (June 1)
Scanning Probe Microscopy: From Fundamentals to Advanced Applications (June 9-12)
Problem Solving with SEM, X-ray Microanalysis, and Electron Backscatter Patterns (June 9-13)
Quantitative X-ray Microanalysis: Problem Solving Using EDS and WDS Techniques (June 9-13)
Analytical Electron Microscopy at the Nanometer Scale (June 9-12)
Focused Ion Beam (FIB) Instrumentation and Applications (June 9-12)
Complete course descriptions and registration form are available at www.Lehigh.edu/microscopy. Contact Sharon Coe (Sharon.coe-at-Lehigh.edu) for more information.
Has anyone encountered problems with fumes escaping from specimen chamber of a Leica Freeze substitution unit ( AFS not AFS2.) during fluid changes.
A new user has noticed, especially when doing changes with Lowicryl HM20, that the fumes are escaping and therefore it is putting the user at risk and others who may be in the area at the time.The built in venting system of the AFS is ducted into a fume hood and appears to be working, at least we can feel air movement from it. Is it possible the fan is not working efficiently ?
In the short term, though not ideal, we are dealing with this by providing users with suitable respirator masks. A more long term/safer solution we are looking into is to have some sort of extract system that will draw fumes away from the user.
If you have encountered this problem before how did you overcome it?
Christine Richardson.
School of Biological & Biomedical Science Centre for Molecular Imaging University of Durham UK.
==============================Original Headers============================== 8, 28 -- From a.c.richardson-at-durham.ac.uk Tue Jan 15 07:18:37 2008 8, 28 -- Received: from hermes1.dur.ac.uk (hermes1.dur.ac.uk [129.234.8.20]) 8, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0FDIaBk008790 8, 28 -- for {Microscopy-at-microscopy.com} ; Tue, 15 Jan 2008 07:18:37 -0600 8, 28 -- Received: from smtphost4.dur.ac.uk (smtphost4.dur.ac.uk [129.234.4.18]) 8, 28 -- by hermes1.dur.ac.uk (8.13.8/8.13.7) with ESMTP id m0FDINM9008765 8, 28 -- for {Microscopy-at-microscopy.com} ; Tue, 15 Jan 2008 13:18:28 GMT 8, 28 -- Received: from weaver.dur.ac.uk (weaver.dur.ac.uk [129.234.4.167]) 8, 28 -- by smtphost4.dur.ac.uk (8.13.7/8.13.7) with ESMTP id m0FDIMVp002984 8, 28 -- for {Microscopy-at-microscopy.com} ; Tue, 15 Jan 2008 13:18:22 GMT 8, 28 -- Received: (from httpd-at-localhost) 8, 28 -- by weaver.dur.ac.uk (8.11.7p3+Sun/8.11.1) id m0FDIMX00491 8, 28 -- for Microscopy-at-microscopy.com; Tue, 15 Jan 2008 13:18:22 GMT 8, 28 -- X-Authentication-Warning: weaver.dur.ac.uk: httpd set sender to dbl0acr-at-smtphost.dur.ac.uk using -f 8, 28 -- Received: from biosci-71.dur.ac.uk (biosci-71.dur.ac.uk [129.234.133.71]) 8, 28 -- by webmailimpa.dur.ac.uk (IMP) with HTTP 8, 28 -- for {dbl0acr-at-imaphost.dur.ac.uk} ; Tue, 15 Jan 2008 13:18:22 +0000 8, 28 -- Message-ID: {1200403102.478cb29e3f019-at-webmailimpa.dur.ac.uk} 8, 28 -- Date: Tue, 15 Jan 2008 13:18:22 +0000 8, 28 -- From: a.c.richardson-at-durham.ac.uk 8, 28 -- To: Microscopy-at-microscopy.com 8, 28 -- Subject: TEM : AFS problem 8, 28 -- MIME-Version: 1.0 8, 28 -- Content-Type: text/plain; charset=ISO-8859-1 8, 28 -- Content-Transfer-Encoding: 8bit 8, 28 -- User-Agent: Internet Messaging Program (IMP) 3.2.1 8, 28 -- X-Originating-IP: 129.234.133.71 8, 28 -- X-DurhamAcUk-MailScanner: Found to be clean, Found to be clean ==============================End of - Headers==============================
here is a description of the position. Please contact me for further inquiries.
Thanks and best regards,
Loic Vilde
Failure Analysis Engineer
Description :
You will apply your scientific and analytical skills to assist in improving product reliability through methodical root cause analysis of failed products.
Your responsibilities will include to design, plan and lead all the investigations necessary to identify the root cause of failed products (field returns or test samples). You will approve, archive and distribute the associated documentation.
You will also work on developing and implementing new investigative procedures specifically aimed at our designs and on organizing failure analysis conclusions in a knowledge database. Our products use diverse materials, from thermoplastics to Titanium parts, with a special emphasis on microelectronics components and printed circuit boards.
You will interface with the Product Development Team members and Manufacturing engineers, as well as with other Environmental Qualification engineers and technical experts from other Schlumberger Technology Centres, and external failure analysis labs.
Equipment used onsite to perform these investigations includes: cold mounting station, dye, precision cut-off machine, semi-automatic grinder/polisher, carbon coater, optical microscopes, variable pressure scanning electron microscope (VP-SEM) with energy-dispersive X-Ray microanalysis (EDS) and X-ray inspection system (Non-Destructive Testing).
Technical, product development, quality management and business related training will be provided.
Notes : International Candidates Will Be Considered. Employer will assist with relocation costs
Requirements :
Education and Qualifications: * PhD in Material Science or MSc with working experience in Failure Analysis domain * Minimum 3 years working experience in similar field * English (working language) Expertise in one or several of the below domains will be highly appreciated : * Scientific/technical experimentation and methods * Analytical investigation procedures, especially sample preparation for SEM/EDS analysis of microelectronics components and electronic assemblies Others : * Ability to organize and document investigations * Strong SEM/EDS and sample preparation skills * Team player with open and direct communication style * Solid computer skills/literacy, especially in data analysis * Effective oral and written communication skills Employer :
WesternGeco, the world's largest geophysical services company, provides comprehensive worldwide reservoir imaging, monitoring, and development services, with the most extensive geophysical survey crews and data processing centers in the industry, as well as the world's largest multiclient data library. Services range from 3D and time-lapse (4D) seismic surveys to multicomponent surveys for delineating prospects and reservoir management as well as electromagnetic surveys.
Loïc VILDE Environmental Qualification / Test and Integration Manager ------------------------------------------------------------- WesternGeco - Oslo Technology Center Schlumberger House Solbråveien 23 N-1372 Asker – Norway
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mckee-at-helix.mgh.harvard.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: mckee-at-helix.mgh.harvard.edu Name: Mary McKee
Organization: MGH
Title-Subject: [Filtered] service for microtomes
Question: Thanks to everyone who responded to my question. You are great!
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both sandeep-at-frontedgetechnology.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Scanning Electron Microscope Repair & Maintenance
Question: Hello All,
We require repair and maintenance services for the SEM & X-ray detector. Please recommend the service providers. We are located in Southern California.
One of my users has been doing some subcellular fractionation of plant cells, and would like to look at the fractions to see if we can identify specific organelles. Are there any specific probes that will help us? I would appreciate any advice! We do have a confocal microscope, which should give us better resolution than a std fluorescence microscope.
Thanks in advance
shea
Dr. S. Shea Miller Agriculture and Agri-Food Canada/Agriculture et Agroalimentaire Canada Telephone/Téléphone: 613-759-1760 Facsimile/Télécopieur: 613-759-1701 Rm 2068 K.W. Neatby Bldg 960 Carling Ave. Central Experimental Farm Ottawa, ON K1A 0C6 millers-at-agr.gc.ca
==============================Original Headers============================== 10, 26 -- From MILLERS-at-AGR.GC.CA Wed Jan 16 15:07:25 2008 10, 26 -- Received: from agrpazsmtp1.agr.gc.ca (agrpazsmtp1.agr.gc.ca [192.197.71.31]) 10, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0GL7OT1009056 10, 26 -- for {Microscopy-at-Microscopy.Com} ; Wed, 16 Jan 2008 15:07:24 -0600 10, 26 -- Received: from onottaxsmtp2.AGR.GC.CA ([10.117.12.122]) 10, 26 -- by agrpazsmtp1.agr.gc.ca (8.13.6/8.13.3) with ESMTP id m0GL7ODl028085 10, 26 -- for {Microscopy-at-Microscopy.Com} ; Wed, 16 Jan 2008 16:07:24 -0500 10, 26 -- Received: from ONOTTAXMS2.AGR.GC.CA ([10.117.12.102]) by onottaxsmtp2.AGR.GC.CA with Microsoft SMTPSVC(6.0.3790.3959); 10, 26 -- Wed, 16 Jan 2008 16:07:24 -0500 10, 26 -- X-MIMEOLE: Produced By Microsoft Exchange V6.5 10, 26 -- Content-class: urn:content-classes:message 10, 26 -- MIME-Version: 1.0 10, 26 -- Content-Type: text/plain; 10, 26 -- charset="iso-8859-1" 10, 26 -- Subject: LM/Confocal- need help identifying plant organelles 10, 26 -- Date: Wed, 16 Jan 2008 16:07:24 -0500 10, 26 -- Message-ID: {FD493497DF264A4E8DC71F1FFE49129D068C69-at-ONOTTAXMS2.AGR.GC.CA} 10, 26 -- X-MS-Has-Attach: 10, 26 -- X-MS-TNEF-Correlator: 10, 26 -- Thread-Topic: LM/Confocal- need help identifying plant organelles 10, 26 -- Thread-Index: AchYg8mtFSj+TLMSTEuQ2ADiaPNl8A== 10, 26 -- From: "Miller, Shea" {MILLERS-at-AGR.GC.CA} 10, 26 -- To: {Microscopy-at-Microscopy.Com} 10, 26 -- X-OriginalArrivalTime: 16 Jan 2008 21:07:24.0256 (UTC) FILETIME=[CA919E00:01C85883] 10, 26 -- Content-Transfer-Encoding: 8bit 10, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m0GL7OT1009056 ==============================End of - Headers==============================
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (jason_jla-at-hotmail.com) from http://microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Wednesday, January 16, 2008 at 11:53:09 ---------------------------------------------------------------------------
Email: jason_jla-at-hotmail.com Name: Jason Alexander
Organization: Immanuel-St. James
Education: K-8 Grade Grammar School
Location: Grand Rapids, MI
Question: Where can I purchase a good microscope that can be used for CELLS and MICROSTRUCTURES OF METALLIC SPECIMENS? I am writing a grant for a microscope and the organization recommended I ask you this question. Thank you for your time?
==============================Original Headers============================== 7, 12 -- From zaluzec-at-ultra5.microscopy.com Wed Jan 16 19:04:36 2008 7, 12 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 7, 12 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0H14Zlu028839 7, 12 -- for {microscopy-at-microscopy.com} ; Wed, 16 Jan 2008 19:04:36 -0600 7, 12 -- Mime-Version: 1.0 7, 12 -- Message-Id: {p06240801c3b45a0b3cb3-at-[206.69.208.22]} 7, 12 -- Date: Wed, 16 Jan 2008 19:04:34 -0600 7, 12 -- To: microscopy-at-microscopy.com 7, 12 -- From: jason_jla-at-hotmail.com (by way of Ask-A-Microscopist) 7, 12 -- Subject: AskAMicroscopist: where to purchase a good microscope that can 7, 12 -- be used for CELLS and MICROSTRUCTURES 7, 12 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers==============================
This Question was submitted to Ask-A-Microscopist by (john.d.williams-at-colostate.edu) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Wednesday, January 16, 2008 at 12:26:42 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both john.d.williams-at-colostate.edu as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Email: john.d.williams-at-colostate.edu Name: Prof. John D. Williams
Organization: Colorado State University
Education: Graduate College
Location: Fort Collins, Colorado, USA
Title: Questions on an ISI-DS130S
Question: I'm being offered a scanning microscope as a donation. It is an ISI-DS130S and was in good working order 2 years ago before being crated up. Does anyone have any detailed information on this scope? I just need a good workhorse for inspecting heavily ion etched surfaces. The last significant contact I've had with an SEM was with Bob Lee at CSU in the late 1980s. So everyone should consider me a newbie. Thanks.
This Question was submitted to Ask-A-Microscopist by (john.d.williams-at-colostate.edu) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Wednesday, January 16, 2008 at 22:03:38 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both john.d.williams-at-colostate.edu as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Email: john.d.williams-at-colostate.edu Name: Prof. John D. Williams
Organization: Colorado State University
Education: Graduate College
Location: Fort Collins, Colorado, USA
Title: ISI-DS130S
Question: Does anyone have an ISI-DS130S operating/maintenance manual they would be willing sell/give me? Thanks, John Williams
Follow-up: I am grateful to Maryah Converse, daughter of Ken Converse of www.qualityimages.biz for translating the descriptions of the images of my SEM calendar "Microstructures 2008". All texts on page 14 are now in English language.
If anybody is able to put the complete calendar PDF www.elektronenmikroskopie.info/calendar_2008.pdf (39 MB) on a server with unrestricted traffic, please do so and let the list know. I will take the file off my server within 12 hours, because I spend all my free transfer volume for this month on my server ;-( If you would only like to get the updated page 14 with the English text, this is the place: www.elektronenmikroskopie.info/calendar_2008_p14.pdf (350 KB)
Best regards, Stefan
} } Dear All, } thanks for the good discussion and the helpful tips to all on the list. } } } With the best wishes for a } happy new year 2008, } success at work and in business, } } Stefan Diller } } } } Please feel free to download and print out my new SEM Calendar 2008 } with images from scanning electron microscopy: } www.elektronenmikroskopie.info/calendar_2008.pdf (39 MB). Images are } Copyright Stefan Diller 2008, please ask for permission prior to any } commercial use. }
Hi all, Happy New Year! Does anyone have experience thick-sectioning watermelon seeds? We're working with mature seeds - black seed coat - unfixed, not embedded. The seeds are left in a moist chamber overnight to imbibe water. Vibratome sectioning didn't work well. Any suggestions? Would fixing and embedding make them easier to section?
I think they're really only good for spitting...but the researcher doesn't want to hear that;-) Any help would be greatly appreciated. Many thanks, Beth
Beth Richardson Plant Biology Department University of Georgia Athens, GA 30602 706-542-1790
==============================Original Headers============================== 4, 19 -- From beth-at-plantbio.uga.edu Thu Jan 17 10:21:22 2008 4, 19 -- Received: from dogwood.plantbio.uga.edu (dogwood.plantbio.uga.edu [128.192.26.2]) 4, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0HGLLmX009715 4, 19 -- for {microscopy-at-microscopy.com} ; Thu, 17 Jan 2008 10:21:21 -0600 4, 19 -- Received: from [128.192.26.46] ([128.192.26.46]) 4, 19 -- (authenticated user beth-at-plantbio.uga.edu) 4, 19 -- by dogwood.plantbio.uga.edu 4, 19 -- (using TLSv1/SSLv3 with cipher AES128-SHA (128 bits)) 4, 19 -- for microscopy-at-microscopy.com; 4, 19 -- Thu, 17 Jan 2008 11:21:15 -0500 4, 19 -- Message-Id: {80AFBBC9-F649-4422-84BA-331CE918C9EB-at-plantbio.uga.edu} 4, 19 -- From: Beth Richardson {beth-at-plantbio.uga.edu} 4, 19 -- To: microscopy microscopy {microscopy-at-microscopy.com} 4, 19 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 4, 19 -- Content-Transfer-Encoding: 7bit 4, 19 -- Mime-Version: 1.0 (Apple Message framework v915) 4, 19 -- Subject: sectioning watermelon seeds 4, 19 -- Date: Thu, 17 Jan 2008 11:22:05 -0500 4, 19 -- X-Mailer: Apple Mail (2.915) ==============================End of - Headers==============================
Hello All, I have a researcher who wants to look at polyurethane coated wires with the SEM. His goal is to see any cracks, breaks or defects in the metal wire encased by the polyurethane. These are very tiny wires, he cannot remove the covering and he needs to look at the wire in sections for defects. We have a super SEM with a backscatter detector but no carbon coater. Any one have experience with this type of sample? How can I prep the samples for the SEM?? Any suggestions would be greatly appreciated!! Thanks! Pat Kysar University of California, Davis Medical School, Pathology EM Lab
==============================Original Headers============================== 3, 21 -- From pekysar-at-ucdavis.edu Thu Jan 17 10:28:28 2008 3, 21 -- Received: from warsaw.ucdavis.edu (warsaw.ucdavis.edu [128.120.32.41]) 3, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0HGSRFu017857 3, 21 -- for {microscopy-at-microscopy.com} ; Thu, 17 Jan 2008 10:28:28 -0600 3, 21 -- Received: from mpat3317 ([169.237.197.34]) 3, 21 -- by warsaw.ucdavis.edu (8.13.7/8.13.1/it-defang-5.4.0) with SMTP id m0HGSQoB007412 3, 21 -- for {microscopy-at-microscopy.com} ; Thu, 17 Jan 2008 08:28:27 -0800 (PST) 3, 21 -- Message-ID: {001701c85926$549f8970$22c5eda9-at-ucdsom.ucdavis.edu} 3, 21 -- From: "Pat Kysar" {pekysar-at-ucdavis.edu} 3, 21 -- To: "Microscopy Listserver" {microscopy-at-microscopy.com} 3, 21 -- Subject: SEM of Wires Encased in Polyurethane 3, 21 -- Date: Thu, 17 Jan 2008 08:30:54 -0800 3, 21 -- MIME-Version: 1.0 3, 21 -- Content-Type: text/plain; 3, 21 -- charset="iso-8859-1" 3, 21 -- Content-Transfer-Encoding: 7bit 3, 21 -- X-Priority: 3 3, 21 -- X-MSMail-Priority: Normal 3, 21 -- X-Mailer: Microsoft Outlook Express 6.00.2800.1914 3, 21 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2800.1914 3, 21 -- X-Scanned-By: MIMEDefang 2.57 on 128.120.32.41 ==============================End of - Headers==============================
Dear All, thanks to Kay at www.quantifoil.com at Jena, Germany the calendar PDF finally moved on to this download address: www.quantifoil.com/calendar_2008.pdf (38 MB) It will be available hopefully the rest of 2008.
If you would only like to get the updated page 14 with the English text, it will stay available here: www.elektronenmikroskopie.info/calendar_2008_p14.pdf (350 KB)
Visit the Geology Department at Davis. They will have a carbon coater.
On Jan 17, 2008, at 11:28 AM, pekysar-at-ucdavis.edu wrote:
} } } } ---------------------------------------------------------------------- } ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } Hello All, } I have a researcher who wants to look at polyurethane coated wires } with the } SEM. His goal is to see any cracks, breaks or defects in the metal } wire } encased by the polyurethane. These are very tiny wires, he cannot } remove the } covering and he needs to look at the wire in sections for defects. } We have a } super SEM with a backscatter detector but no carbon coater. Any one } have } experience with this type of sample? How can I prep the samples for } the } SEM?? Any suggestions would be greatly appreciated!! Thanks! } Pat Kysar } University of California, Davis } Medical School, Pathology } EM Lab } } ---------------------------------
Gordon L. Nord, Ph. D Senior Scientist International Environmental Research Foundation New York, NY
{http://www.ierfinc.org/}
==============================Original Headers============================== 5, 24 -- From gnord-at-mindspring.com Thu Jan 17 11:19:54 2008 5, 24 -- Received: from elasmtp-mealy.atl.sa.earthlink.net (elasmtp-mealy.atl.sa.earthlink.net [209.86.89.69]) 5, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0HHJsFl009275 5, 24 -- for {Microscopy-at-MSA.Microscopy.com} ; Thu, 17 Jan 2008 11:19:54 -0600 5, 24 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 5, 24 -- s=dk20050327; d=mindspring.com; 5, 24 -- b=sqpdoPTjE2p9akbrDPm5qWkCegFk57dghjW7lK6jTZszqv/ywq3qOM4hA51VyPIF; 5, 24 -- h=Received:Mime-Version:In-Reply-To:References:Content-Type:Message-Id:Content-Transfer-Encoding:From:Subject:Date:To:X-Mailer:X-ELNK-Trace:X-Originating-IP; 5, 24 -- Received: from [71.171.97.30] (helo=[10.0.1.198]) 5, 24 -- by elasmtp-mealy.atl.sa.earthlink.net with asmtp (Exim 4.34) 5, 24 -- id 1JFYPB-0003Pn-L2; Thu, 17 Jan 2008 12:19:53 -0500 5, 24 -- Mime-Version: 1.0 (Apple Message framework v753) 5, 24 -- In-Reply-To: {200801171628.m0HGSoN1019030-at-ns.microscopy.com} 5, 24 -- References: {200801171628.m0HGSoN1019030-at-ns.microscopy.com} 5, 24 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 5, 24 -- Message-Id: {3B5CEB15-5891-4CC9-9762-AF690DE41DB5-at-mindspring.com} 5, 24 -- Content-Transfer-Encoding: 7bit 5, 24 -- From: Gordon Nord {gnord-at-mindspring.com} 5, 24 -- Subject: Re: [Microscopy] SEM of Wires Encased in Polyurethane 5, 24 -- Date: Thu, 17 Jan 2008 12:19:52 -0500 5, 24 -- To: pekysar-at-ucdavis.edu, Microscopy-at-MSA.Microscopy.com 5, 24 -- X-Mailer: Apple Mail (2.753) 5, 24 -- X-ELNK-Trace: 3df33169c923931ad4c20f6b8d69d8885d2a9c731cc891172964ec30db4ae644ef813bcc8d13dfc1350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c 5, 24 -- X-Originating-IP: 71.171.97.30 ==============================End of - Headers==============================
Greetings, This is the first announcement for students who will attend the Microscopy and Microanalysis Meeting in Albuquerque, Aug.3-7 and would like to apply for a Student Bursary to help defray meeting costs. The complete description of the Bursary is listed below, including the registration process.
The contact person this year that will coordinate the initial student bursary sign-up is: Amanda Lawrence (alawrence-at-entomology.msstate.edu ). Clayton Lohen (clohen-at-vt.edu) will be helping with coordination of bursary activities once on site.
STUDENT BURSARIES The Microscopy Society of America values student members and recognizes that they are the future of both the society and the field of microscopy in general. MSA is therefore pleased to offer student bursaries of $200 to registered students. The most important purpose of these bursaries is to encourage students to attend the annual MSA/MAS Microscopy and Microanalysis meeting, where these young scientists can meet and interact with the established microscopy community as well as assisting with the meeting.
Each bursary recipient will be expected to work for a minimum of 20 hours during the meeting and/or at the pre-meeting weekend events. A student may work up to an additional 20 hours, for a total of 40 hours. These extra hours will add to the bursary total at a rate of $10 per hour. The maximum bursary will therefore be $400. The duties will involve, but are not necessarily limited to, providing support in symposia (helping with audio-visual needs, maintaining an attendance count, and helping speakers set up for their presentation), staffing the MSA Megabooth, and monitoring use of the Internet Cafe. Applicants for the bursaries must be members of MSA or MAS, and enrolled as students at a recognized educational institution. All MSA or MAS student members are eligible for bursaries, including those who are recipients of MSA Presidential Scholars Awards and MAS Distinguished Scholar Awards. Eligible on the conference website, or at on-site registration. Bursaries are limited and early application is encouraged. Don't forget to check the website for special student housing discounts as well.
How it works: The registration form for the meeting will have a check box indicating that the applicant is a registered student and is requesting a bursary. The check box will have a note beside it reminding the applicant that the bursary requires them to work at the meeting.
Students who have applied for bursaries will be contacted by the initial student bursary co-coordinator (Amanda) to schedule meeting tasks prior to arrival in Albuquerque. The student is then expected to fulfill their assigned tasks and will be issued forms which must be signed by all of the contact persons listed, indicating that all assigned tasks have been performed. Upon completion of assignments and submission of the signed forms, the bursary check will be issued by the appropriate LAC representative.
We would also like to solicit volunteers from 'non-students' as well to help with meeting activities.
Should you have questions , please contact:
Amanda Lawrence (alawrence-at-entomology.msstate.edu) Electron Microscope Center 100 Twelve Lane Mississippi State University Mississippi State, MS 39762 (662)-325-3019 Work (662)-325-0246 Fax
==============================Original Headers============================== 13, 20 -- From ALawrence-at-entomology.msstate.edu Thu Jan 17 13:14:13 2008 13, 20 -- Received: from mailhost2.groupwise.msstate.edu (mailhost2.groupwise.msstate.edu [130.18.2.186]) 13, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0HJECAA000748 13, 20 -- for {microscopy-at-microscopy.com} ; Thu, 17 Jan 2008 13:14:13 -0600 13, 20 -- Received: from Gateway2-MTA by mailhost2.groupwise.msstate.edu 13, 20 -- with Novell_GroupWise; Thu, 17 Jan 2008 13:14:11 -0600 13, 20 -- Message-Id: {478F549A.B26A.00E6.0-at-entomology.msstate.edu} 13, 20 -- X-Mailer: Novell GroupWise Internet Agent 7.0.2 13, 20 -- Date: Thu, 17 Jan 2008 13:14:02 -0600 13, 20 -- From: "Amanda M. Lawrence" {ALawrence-at-entomology.msstate.edu} 13, 20 -- To: {microscopy-at-microscopy.com} 13, 20 -- Subject: M&M 2008 student bursary and volunteer request 13, 20 -- References: {478F515B020000E6000314B8-at-mailhost2.groupwise.msstate.edu} 13, 20 -- {478F520B020000E6000314BB-at-mailhost2.groupwise.msstate.edu} 13, 20 -- {478F549A020000E6000314BE-at-mailhost2.groupwise.msstate.edu} 13, 20 -- Mime-Version: 1.0 13, 20 -- Content-Type: text/plain; charset=US-ASCII 13, 20 -- Content-Disposition: inline 13, 20 -- Content-Transfer-Encoding: 8bit 13, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m0HJECAA000748 ==============================End of - Headers==============================
I have several questions that may allow you to provide more specific information useful to the listserver community: Is the polyurethane cross-linked? Does he need to examine the wire in cross-section or might examination of the surface of the wire suffice? If a cross-section is required, which plane of the wire does he want to examine, i.e. the axial cross-section or the longitudinal cross-section? How do you intend to prepare the cross-sections: grinding and polishing; microtomy; FIB, etc?
If the polyurethane is not cross-linked, I suggest you consider dissolving it in a good solvent, thus leaving the bare wires available for subsequent sample preparation and microscopy.
Best regards,
Disclaimer: The comments and opinions given above are those of the author alone and do not represent any position of ExxonMobil Chemical Company.
Gary M. Brown Microscopy Specialist ExxonMobil Chemical Company Baytown Technology & Engineering - West 5200 Bayway Drive Baytown, Texas 77520-2101 phone: 281 834 2387 fax: 281 834 2395 e-mail: Gary.M.Brown-at-ExxonMobil.com
"Our business in life is not to succeed, but to continue to fail in good spirits." Robert Louis Stevenson
gnord-at-mindspri ng.com To gary.m.brown-at-exxonmobil.com 01/17/08 11:21 cc AM Subject [Microscopy] Re: SEM of Wires Please respond Encased in Polyurethane to gnord-at-mindspri ng.com
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Visit the Geology Department at Davis. They will have a carbon coater.
On Jan 17, 2008, at 11:28 AM, pekysar-at-ucdavis.edu wrote:
} } } } ---------------------------------------------------------------------- } ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } Hello All, } I have a researcher who wants to look at polyurethane coated wires } with the } SEM. His goal is to see any cracks, breaks or defects in the metal } wire } encased by the polyurethane. These are very tiny wires, he cannot } remove the } covering and he needs to look at the wire in sections for defects. } We have a } super SEM with a backscatter detector but no carbon coater. Any one } have } experience with this type of sample? How can I prep the samples for } the } SEM?? Any suggestions would be greatly appreciated!! Thanks! } Pat Kysar } University of California, Davis } Medical School, Pathology } EM Lab } } ---------------------------------
Gordon L. Nord, Ph. D Senior Scientist International Environmental Research Foundation New York, NY
{http://www.ierfinc.org/}
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==============================Original Headers============================== 33, 19 -- From gary.m.brown-at-exxonmobil.com Thu Jan 17 14:42:52 2008 33, 19 -- Received: from dalspc01.exxonmobil.com (dalspc01.exxonmobil.com [131.126.223.1]) 33, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0HKgohL016608 33, 19 -- for {microscopy-at-microscopy.com} ; Thu, 17 Jan 2008 14:42:51 -0600 33, 19 -- Received: from dalnmg04.na.xom.com (dalnmg04.na.xom.com [131.126.97.123]) 33, 19 -- by dalspc01.exxonmobil.com (Switch-3.1.11/Switch-3.1.11) with ESMTP id m0HKgmo7011902; 33, 19 -- Thu, 17 Jan 2008 14:42:48 -0600 (CST) 33, 19 -- In-Reply-To: {200801171721.m0HHLh8q015030-at-ns.microscopy.com} 33, 19 -- Subject: Re: [Microscopy] Re: SEM of Wires Encased in Polyurethane 33, 19 -- Importance: 33, 19 -- To: gnord-at-mindspring.com, microscopy-at-microscopy.com 33, 19 -- X-Mailer: Lotus Notes 652HF109 January 14, 2005 33, 19 -- Message-ID: {OF7191A216.E3A105D0-ON862573D3.006F4302-862573D3.0071C2DC-at-exxonmobil.com} 33, 19 -- From: gary.m.brown-at-exxonmobil.com 33, 19 -- Date: Thu, 17 Jan 2008 14:42:34 -0600 33, 19 -- X-MIMETrack: Serialize by Router on Dalnmg04.na.xom.com/S/ExxonMobil(652FP1HF193|March 33, 19 -- 02, 2006) at 01/17/2008 02:42:48 PM 33, 19 -- MIME-Version: 1.0 33, 19 -- Content-type: text/plain; charset=US-ASCII ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mgengle-at-uky.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: mgengle-at-uky.edu Name: Mary Gail Engle
Organization: Imaging Facility, University of Kentucky
Title-Subject: [Filtered] Microwave processing for TEM
Question: Having just attended a microwave workshop and being impressed with the morphology of the tissue for "normal" EM, I was wondering if any of you have experience with, or an opinion regarding the quality of colloidal gold immunolabeling using the microwave. Speed is not necessarily an issue for us, but if we could get better morphology as well as good gold labeling, we would consider purchasing the instrument.
Thank you, Mary Gail Engle, Manager, Imaging Facility University of KY
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Email: atn5613-at-rit.edu Name: Algis N
Organization: Rochester Institute of Technology
Title-Subject: [Filtered] Target glue for PSD
Question: Hello All, I am doing some research using a plasma sputtering device (VCR Group IBS TM200S)to sputter metals onto a substrate. What type of adhesive should I use to adhere the metal foil targets I will be using to the target holder? Should it be conductive and what contamination concerns should I be looking for? Thanks. Algis
Pat, I'm a little confused by "he needs to look at the wire in sections". If you mean that the wire will be cross-sectioned, then advice has already been given: mount, grind and polish. If, on the other hand, you mean that short, perhaps specific, sections will be examined for surface defects on the metal wire and "he cannot remove the covering", my question is: how thick is the polyurethane insulation? If it's only a couple of microns thick, try going to your maximum kV and see if the beam will penetrate the insulation. BSE may give the best image, but SE might also work. If the insulation is too thick for beam penetration, you're left with either stripping or sectioning.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: pekysar-at-ucdavis.edu [mailto:pekysar-at-ucdavis.edu] Sent: Thursday, January 17, 2008 11:31 AM To: kenconverse-at-qualityimages.biz
Hello All, I have a researcher who wants to look at polyurethane coated wires with the SEM. His goal is to see any cracks, breaks or defects in the metal wire encased by the polyurethane. These are very tiny wires, he cannot remove the covering and he needs to look at the wire in sections for defects. We have a super SEM with a backscatter detector but no carbon coater. Any one have experience with this type of sample? How can I prep the samples for the SEM?? Any suggestions would be greatly appreciated!! Thanks! Pat Kysar University of California, Davis Medical School, Pathology EM Lab
==============================Original Headers============================== 3, 21 -- From pekysar-at-ucdavis.edu Thu Jan 17 10:28:28 2008 3, 21 -- Received: from warsaw.ucdavis.edu (warsaw.ucdavis.edu [128.120.32.41]) 3, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0HGSRFu017857 3, 21 -- for {microscopy-at-microscopy.com} ; Thu, 17 Jan 2008 10:28:28 -0600 3, 21 -- Received: from mpat3317 ([169.237.197.34]) 3, 21 -- by warsaw.ucdavis.edu (8.13.7/8.13.1/it-defang-5.4.0) with SMTP id m0HGSQoB007412 3, 21 -- for {microscopy-at-microscopy.com} ; Thu, 17 Jan 2008 08:28:27 -0800 (PST) 3, 21 -- Message-ID: {001701c85926$549f8970$22c5eda9-at-ucdsom.ucdavis.edu} 3, 21 -- From: "Pat Kysar" {pekysar-at-ucdavis.edu} 3, 21 -- To: "Microscopy Listserver" {microscopy-at-microscopy.com} 3, 21 --
Ken,
Unfortunately, many technologists are put in your position. That is, one may be asked to analyze potentially complex, multi-component materials without substantive guidance from the requestor, in areas outside one's expertise, and with little help within our immediate technical community.
I encourage you to put some of the responsibility back onto the requestor. He/she should be able to obtain information on the composition of the polyurethane insulation from the manufacturer. If the requestor is not willing to spend any time or effort to work the problem, just how important can it be? The last point I would make regards the scope of your technical capabilities. Specifically, do you have the sample preparation and microscopy capabilities that will be needed in the analysis of this material.
Best regards,
Disclaimer: The comments and opinions given above are those of the author alone and do not represent any position of ExxonMobil Chemical Company.
Gary M. Brown Microscopy Specialist ExxonMobil Chemical Company Baytown Technology & Engineering - West 5200 Bayway Drive Baytown, Texas 77520-2101 phone: 281 834 2387 fax: 281 834 2395 e-mail: Gary.M.Brown-at-ExxonMobil.com
"Our business in life is not to succeed, but to continue to fail in good spirits." Robert Louis Stevenson
kenconverse-at-qu alityimages.bi z To gary.m.brown-at-exxonmobil.com cc 01/18/08 02:55 PM Subject [Microscopy] RE: SEM of Wires Encased in Polyurethane Please respond to kenconverse-at-qu alityimages.bi z
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Pat, I'm a little confused by "he needs to look at the wire in sections". If you mean that the wire will be cross-sectioned, then advice has already been given: mount, grind and polish. If, on the other hand, you mean that short, perhaps specific, sections will be examined for surface defects on the metal wire and "he cannot remove the covering", my question is: how thick is the polyurethane insulation? If it's only a couple of microns thick, try going to your maximum kV and see if the beam will penetrate the insulation. BSE may give the best image, but SE might also work. If the insulation is too thick for beam penetration, you're left with either stripping or sectioning.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: pekysar-at-ucdavis.edu [mailto:pekysar-at-ucdavis.edu] Sent: Thursday, January 17, 2008 11:31 AM To: kenconverse-at-qualityimages.biz
Hello All, I have a researcher who wants to look at polyurethane coated wires with the SEM. His goal is to see any cracks, breaks or defects in the metal wire encased by the polyurethane. These are very tiny wires, he cannot remove the covering and he needs to look at the wire in sections for defects. We have a super SEM with a backscatter detector but no carbon coater. Any one have experience with this type of sample? How can I prep the samples for the SEM?? Any suggestions would be greatly appreciated!! Thanks! Pat Kysar University of California, Davis Medical School, Pathology EM Lab
==============================Original Headers============================== 3, 21 -- From pekysar-at-ucdavis.edu Thu Jan 17 10:28:28 2008 3, 21 -- Received: from warsaw.ucdavis.edu (warsaw.ucdavis.edu [128.120.32.41]) 3, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0HGSRFu017857 3, 21 -- for {microscopy-at-microscopy.com} ; Thu, 17 Jan 2008 10:28:28 -0600 3, 21 -- Received: from mpat3317 ([169.237.197.34]) 3, 21 -- by warsaw.ucdavis.edu (8.13.7/8.13.1/it-defang-5.4.0) with SMTP id m0HGSQoB007412 3, 21 -- for {microscopy-at-microscopy.com} ; Thu, 17 Jan 2008 08:28:27 -0800 (PST) 3, 21 -- Message-ID: {001701c85926$549f8970$22c5eda9-at-ucdsom.ucdavis.edu} 3, 21 -- From: "Pat Kysar" {pekysar-at-ucdavis.edu} 3, 21 -- To: "Microscopy Listserver" {microscopy-at-microscopy.com} 3, 21 --
I have been using a Hitachi 4800 SEM to image E. coli bacteria, but am not sure if what I've been seeing is representative of good fixation or sample preparation.
So far I've used a number of different fixatives (both with and without microwave assistance), the latest being 1% PFA, 1% GA, 50 mM cacodylate, pH 7.4. I have not been using any osmium. My main concern comes from the fact that the E. coli always look a bit bumpy and textured, not smooth and featureless like the B. subtilis I have also been imaging with more success. Also, I almost never see flagella.
My question is if anyone has recommendations for some resources in which I could find high resolution images of high-quality E. coli samples. When I try doing a Google Images search, or a quick look at PubMed, the micrographs I find are of considerably less resolution than I typically get with the Hitachi 4800 I've been using, so I'm never sure if the surface texture is normal and expected or completely anomalous.
Many thanks, Nate Chongsiriwatana
Graduate Student Northwestern University Department of Chemical and Biological Engineering
==============================Original Headers============================== 5, 20 -- From nathanielchongsiriwatana2008-at-u.northwestern.edu Fri Jan 18 16:06:13 2008 5, 20 -- Received: from py-out-1112.google.com (py-out-1112.google.com [64.233.166.177]) 5, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0IM6DdZ004719 5, 20 -- for {microscopy-at-microscopy.com} ; Fri, 18 Jan 2008 16:06:13 -0600 5, 20 -- Received: by py-out-1112.google.com with SMTP id p76so1642904pyb.6 5, 20 -- for {microscopy-at-microscopy.com} ; Fri, 18 Jan 2008 14:06:13 -0800 (PST) 5, 20 -- Received: by 10.110.39.5 with SMTP id m5mr2356250tim.55.1200693971970; 5, 20 -- Fri, 18 Jan 2008 14:06:11 -0800 (PST) 5, 20 -- Received: by 10.70.31.17 with HTTP; Fri, 18 Jan 2008 14:06:11 -0800 (PST) 5, 20 -- Message-ID: {2d96bb2a0801181406j256d4e1asc419ea1e6006dba6-at-mail.gmail.com} 5, 20 -- Date: Fri, 18 Jan 2008 16:06:11 -0600 5, 20 -- From: "Nathaniel Chongsiriwatana" {npchong-at-northwestern.edu} 5, 20 -- Sender: nathanielchongsiriwatana2008-at-u.northwestern.edu 5, 20 -- To: microscopy-at-microscopy.com 5, 20 -- Subject: SEM - resources for images of well-prepared E. coli? 5, 20 -- MIME-Version: 1.0 5, 20 -- Content-Type: text/plain; charset=ISO-8859-1 5, 20 -- Content-Transfer-Encoding: 7bit 5, 20 -- Content-Disposition: inline 5, 20 -- X-Google-Sender-Auth: b4be85d1eb58bf35 ==============================End of - Headers==============================
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Email: vavv2009-at-yahoo.com Name: Arnold Villanueva
Question: I am wondering where one can get education (or some type of college degree or certification) to become a Microscopist .. for example, how does one obtain an associates degree (or certificate or Bachelor's) in Microscopy? any information would be greatly appreciated .. thank you!
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Email: pgan-at-ap.ansell.com Name: Gan Phay Fang
Organization: Ansell Shah Alam Sdn Bhd
Title-Subject: [Filtered] ISO 17025 Accreditation on SEM/EDX Analysis
Question: We are thinking of getting an ISO 17025 Accreditation on the SEM/EDX analysis. Our analysis is mostly getting the SEM micrographs and EDX analysis on the polymeric materials. Any advice and comments on starting up the preparation for the ISO 17025 Accreditation are much appreciated.
An Associates degree as an electron microscopy technician is available from the Madison Area Technical College (MATC) in Madison, WI, as well as from the San Joaquin Delta College of Stockton, CA. Central Michigan University in Mount Pleasant, MI also has an excellent program that combines an electron microscopy emphasis with an undergraduate biology degree. These are the programs that I am aware of.
Yours, Matthew Stephenson Impact Analytical/Michigan Molecular Institute
-----Original Message----- X-from: vavv2009-at-yahoo.com [mailto:vavv2009-at-yahoo.com] Sent: Monday, January 21, 2008 9:41 AM To: stephenson-at-impactanalytical.com
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please copy both vavv2009-at-yahoo.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: vavv2009-at-yahoo.com Name: Arnold Villanueva
Question: I am wondering where one can get education (or some type of college degree or certification) to become a Microscopist .. for example, how does one obtain an associates degree (or certificate or Bachelor's) in Microscopy? any information would be greatly appreciated .. thank you!
Gan, is accreditation really needed? When our quality system was staring up and gelling (QS 9000, ISO 17025, now TS 16949), there was a lot of discussion in our company early on about how our SEM would fit in. The end result is that our SEM and EDS are not in our company's quality system. It is more of a diagnostic tool and it doesn't factor in to the production of our product (bearings). Our unit has a sticker on it that says "Not for product conformance".
Face it, The SEM is just an imaging device like a fancy camera. Are your cameras in the quality system?... probably not. Some people think because they have a highly expensive and technical piece of equipment, that it needs to be in the quality system. If you're measuring something, then this issue merits consideration.
As far as EDS analysis goes, I report semiquantitative results without numbers. My results are described in words, because numbers imply accuracy, and any quality auditor (or client) will want to know the error limits of reported numbers.
Stu Smalinskas, P.E. SKF North American Technical Center Plymouth, Michigan (734) 414-6862
--- pgan-at-ap.ansell.com wrote: } Email: pgan-at-ap.ansell.com } Name: Gan Phay Fang } } Organization: Ansell Shah Alam Sdn Bhd } } Title-Subject: [Filtered] ISO 17025 Accreditation on } SEM/EDX Analysis } } Question: We are thinking of getting an ISO 17025 } Accreditation on the SEM/EDX analysis. Our analysis } is mostly getting the SEM micrographs and EDX } analysis on the polymeric materials. Any advice and } comments on starting up the preparation for the ISO } 17025 Accreditation are much appreciated. } }
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==============================Original Headers============================== 7, 20 -- From smalinskas-at-yahoo.com Mon Jan 21 09:54:49 2008 7, 20 -- Received: from web34406.mail.mud.yahoo.com (web34406.mail.mud.yahoo.com [66.163.178.155]) 7, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m0LFsm45023827 7, 20 -- for {microscopy-at-sparc5.microscopy.com} ; Mon, 21 Jan 2008 09:54:48 -0600 7, 20 -- Received: (qmail 58235 invoked by uid 60001); 21 Jan 2008 15:54:48 -0000 7, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 7, 20 -- s=s1024; d=yahoo.com; 7, 20 -- h=X-YMail-OSG:Received:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 7, 20 -- b=iEnq4bAb1z8p8Sk8JYubgMOeyi+uZflUVWAg6+q/xTZr+dzf8O4+tlbv1u/2xly57GcPG/sZpCZ+uCXEoT48meUmi+c8Cw3D7e+7APMZN3vXAr2V7MWaeN75DuP+66cIQUHGbszTs8XG2wRHKhiUE4Eo3cBWmfT89TIrZ3znGcA=; 7, 20 -- X-YMail-OSG: Yj6FY.IVM1mqdKVW3.jO6.Gj1f3_mqyzPnprbOx_pMkB3.6VgG6Hd0cVTRjjFZ1PV_9Yps8LMcBxrWpebSjtr2xMpTCzrx0WOfh7kt_VUUlxUmkVg.A- 7, 20 -- Received: from [141.151.33.213] by web34406.mail.mud.yahoo.com via HTTP; Mon, 21 Jan 2008 07:54:47 PST 7, 20 -- Date: Mon, 21 Jan 2008 07:54:47 -0800 (PST) 7, 20 -- From: Kestutis Smalinskas {smalinskas-at-yahoo.com} 7, 20 -- Subject: Re: [Microscopy] viaWWW: ISO 17025 Accreditation on SEM/EDX Analysis 7, 20 -- To: pgan-at-ap.ansell.com, microscopy-at-ns.microscopy.com 7, 20 -- In-Reply-To: {200801211437.m0LEb8lF018849-at-ns.microscopy.com} 7, 20 -- MIME-Version: 1.0 7, 20 -- Content-Type: text/plain; charset=iso-8859-1 7, 20 -- Content-Transfer-Encoding: 8bit 7, 20 -- Message-ID: {928897.43407.qm-at-web34406.mail.mud.yahoo.com} ==============================End of - Headers==============================
FEI servicemen recommend using only distilled or deionized (DI) water in chilled water recirculator systems.
A very effective algae killer is a long chain quaternary ammonium salt surfactant that you can buy at any quality swimming pool dealer. IMO, these quat's are much much better at killing algae than adding available chlorine from whatever chlorine source. The dosage rate for the very long chained surfactant is something like one quart per 10,000 gallon of water. That works out to a low PPM level of a biocide surfactant. Adding a chlorine-based chemical generates soluble chloride ions that can damage and can pit corrode stainless steel in DP tubing or SS fittings with O-ring channels.
There is another side to all this but this next side is rarely discussed. Algae problems are always discussed on the list server.
If you are running pure DI water, then how can your microscope ever plug up from green scale? On July 30, 2007 I emailed my FEI serviceman with 25-30 years of microscope experience with Philips-FEI and asked about algae and scaling. He emailed me the answers. No microscopes that they were asked to unplug were ever plugged up from algae. It was always green scale that caused the plugging and the scale was removed with a weak organic acid.
Here are the two sides of the chiller water problem in equation form.
This is the standard belief of the cause of green chiller water and is shown below as a simplified photosynthesis equation. Quat's efficiently interrupt the algae cycle on the left-hand side of the equation by breaking open algae cell walls and killing them. So they never really have a chance to multiply in a properly run system. Dissociated hypochlorous acid (HClO) from any chlorine source kills them too but generates additional chloride ions.
Notice that CO2 is involved in both reactions. Killing or preventing algae with a surfactant, an available chlorine oxidizer (hypochlorous acid), or stopping sunlight will eventually stop the first reaction. Stopping reaction one only shifts the chemistry focus to reaction two. The problem is that large stirred chiller systems dissolve more O2 and CO2 at higher rates than smaller chilled water systems, in my experience. These gases are dissolved in the pure DI water from the stirring and/or air exposure by the chiller's unsealed and open reservoir tank of water. Reaction two is slow and is normally unnoticed until the microscope plugs up. It takes awhile to notice the copper ion build up unless you regularly test the chilled water for PPM levels of copper by AA spectroscopy to determine the rate of copper ion buildup. Furthermore, short light paths of one to three centimeters or looking through a 6 mm piece of transparent tubing is just not that effective in spotting the first stages of soluble copper ion build up by trying to detect a faint initial green color.
I could say much more about the mechanisms, the effect of copper surface area of exposure, scale distribution in pipes, mechanical devices to add, and how the Ksp of copper carbonate enters into all this. Here are simple tests and hints to sort out the situation.
1. Test One. Adding a quaternary ammonium salt surfactant to green water will kill algae in about an hour. You can use excessive hypochlorite ion (bleach) on a chiller water sample also. A loss of green color means that a lot of algae were present. 2. Test Two. Adding ammonium hydroxide to a sample of green chiller water will turn any dissolved PPM levels of copper ions dark blue at a very high pH. A positive dark blue copper complex color means that soluble copper ions are being formed and those ions are tinting the water green. 3. BOTH of these two tests should be done to sort out what your system is doing. 4. PPM levels of copper ions will kill algae. 5. So you should either have a green algae problem or you have a green dissolved copper ion problem. When the copper ions build up in solution from the continuous dissolution of CO2 and O2, they will exceed the copper carbonate Ksp and start to precipitate the carbonate to form the green basic copper carbonate scale shown above. 6. In order to prevent scale formation, you MUST periodically drain out 25%-50% of the chiller water every 3-6 months. This draining and refilling action is another way to prevent the copper ions and carbonate ions from exceeding the Ksp value of copper carbonate, which has a lower Ksp than CaCO3. 7. If forced to use city water, you should flush out the "hard" city water in instrument dead volumes with DI water to help avoid any mixed carbonate scaling involving Ca and Mg. 8. Items 6 & 7 almost make an automatic DI water refill system at the reservoir tank mandatory in large systems. It only has to run when you will be flushing, refilling, or performing some other planned loss of chilled water.
Disclaimer: The way additional mechanical features and the chemistry interact can vary a lot in a custom installed central system and with the various needs of differnet user's instruments. It is important that users of central chilled water recirculators understand how their actions, such as flushing with city water, can have an impact on other users. IMO, this training is absolutely necessary for users and needed to make facility engineers understand why certain devices added to a central system are needed. This carbonate is the green corrosion product that slowly forms on copper roofs and gutters from only air and water exposure. The first thin brown scale color on copper roofs and in pipes is black to dark-brown copper oxide (CuO). After that forms and with further CO2 exposure, reaction two is followed.
HTH,
Paul Beauregard Senior Research Associate, retired Greensburg, PA 724-834-2247
==============================Original Headers============================== 17, 28 -- From beaurega-at-westol.com Mon Jan 21 11:22:28 2008 17, 28 -- Received: from smtp-gateway-5.winbeam.com (smtp-gateway-5.winbeam.com [64.84.97.70]) 17, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0LHMRe1009250 17, 28 -- for {microscopy-at-microscopy.com} ; Mon, 21 Jan 2008 11:22:28 -0600 17, 28 -- X-Winbeam-MailScanner-Watermark: 1201540666.03028-at-rbmMn+OtGx2c+3wAVSvNqg 17, 28 -- Received: from mail.winbeam.com (mail.winbeam.com [64.84.96.10]) 17, 28 -- by smtp-gateway-5.winbeam.com (8.13.2/8.12.8) with SMTP id m0LHHT8h024130 17, 28 -- for {microscopy-at-microscopy.com} ; Mon, 21 Jan 2008 12:17:30 -0500 17, 28 -- Received: (qmail 17002 invoked by uid 89); 21 Jan 2008 17:17:29 -0000 17, 28 -- Received: from pitts-69-72-117-190.dynamic-dialup.coretel.net (HELO millenium) (69.72.117.190) 17, 28 -- by mail.winbeam.com with SMTP; 21 Jan 2008 17:17:29 -0000 17, 28 -- Message-Id: {3.0.6.32.20080121121728.007b6560-at-pop3.norton.antivirus} 17, 28 -- X-Sender: beaurega/mail.westol.com-at-pop3.norton.antivirus (Unverified) 17, 28 -- X-Mailer: QUALCOMM Windows Eudora Light Version 3.0.6 (32) 17, 28 -- Date: Mon, 21 Jan 2008 12:17:28 -0500 17, 28 -- To: microscopy-at-microscopy.com 17, 28 -- From: Beaurega {beaurega-at-westol.com} 17, 28 -- Subject: [Microscopy] RE: Product for watercooling 17, 28 -- Mime-Version: 1.0 17, 28 -- Content-Type: text/plain; charset="iso-8859-1" 17, 28 -- Content-Transfer-Encoding: 8bit 17, 28 -- X-Winbeam-MailScanner-Information: Winbeam - Please contact Technical Support for more information 17, 28 -- X-Winbeam-MailScanner: Found to be clean Winbeam (courtesy of MailScanner) 17, 28 -- X-Winbeam-MailScanner-SpamCheck: not spam (whitelisted), 17, 28 -- SpamAssassin (not cached, score=-5.139, required 4, 17, 28 -- autolearn=not spam, AWL 0.36, BAYES_00 -0.50, local_FROM_WB -1.00, 17, 28 -- local_HAM_FROM_WB -4.00) 17, 28 -- X-Winbeam-MailScanner-From: beaurega-at-westol.com ==============================End of - Headers==============================
I only have experience in sectioning seeds of Orobanche cumana. I was successful doing semi- and ultrathin sections after embedding in LR-White. The first prepartion step was to perforate the seeds with a fine needle. Without perforating the seed coat, it was impossible to get anything into these tiny seeds.
I think seeds are always diffcult, because their coat is quite tight, their water content extremely low, and they store masses of starch grains or other storage material which are difficult to fix and to section. Watermelon seeds are quite large. Maybe you can cut them and try to embed the pieces?
Good Luck, Anne
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi all, } Happy New Year! } Does anyone have experience thick-sectioning watermelon seeds? We're } working with mature seeds - black seed coat - unfixed, not embedded. } The seeds are left in a moist chamber overnight to imbibe water. } Vibratome sectioning didn't work well. Any suggestions? Would fixing } and embedding make them easier to section? } } I think they're really only good for spitting...but the researcher } doesn't want to hear that;-) } Any help would be greatly appreciated. } Many thanks, } Beth } } Beth Richardson } Plant Biology Department } University of Georgia } Athens, GA 30602 } 706-542-1790 } } } ==============================Original Headers============================== } 4, 19 -- From beth-at-plantbio.uga.edu Thu Jan 17 10:21:22 2008 } 4, 19 -- Received: from dogwood.plantbio.uga.edu (dogwood.plantbio.uga.edu } [128.192.26.2]) } 4, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } m0HGLLmX009715 } 4, 19 -- for {microscopy-at-microscopy.com} ; Thu, 17 Jan 2008 10:21:21 -0600 } 4, 19 -- Received: from [128.192.26.46] ([128.192.26.46]) } 4, 19 -- (authenticated user beth-at-plantbio.uga.edu) } 4, 19 -- by dogwood.plantbio.uga.edu } 4, 19 -- (using TLSv1/SSLv3 with cipher AES128-SHA (128 bits)) } 4, 19 -- for microscopy-at-microscopy.com; } 4, 19 -- Thu, 17 Jan 2008 11:21:15 -0500 } 4, 19 -- Message-Id: {80AFBBC9-F649-4422-84BA-331CE918C9EB-at-plantbio.uga.edu} } 4, 19 -- From: Beth Richardson {beth-at-plantbio.uga.edu} } 4, 19 -- To: microscopy microscopy {microscopy-at-microscopy.com} } 4, 19 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; } delsp=yes } 4, 19 -- Content-Transfer-Encoding: 7bit } 4, 19 -- Mime-Version: 1.0 (Apple Message framework v915) } 4, 19 -- Subject: sectioning watermelon seeds } 4, 19 -- Date: Thu, 17 Jan 2008 11:22:05 -0500 } 4, 19 -- X-Mailer: Apple Mail (2.915) } ==============================End of - Headers============================== }
==============================Original Headers============================== 7, 32 -- From heller-at-uni-hohenheim.de Mon Jan 21 14:44:01 2008 7, 32 -- Received: from smtp1.rz.uni-hohenheim.de (smtp1.rz.uni-hohenheim.de [144.41.4.41]) 7, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0LKi0pI030518 7, 32 -- for {Microscopy-at-microscopy.com} ; Mon, 21 Jan 2008 14:44:01 -0600 7, 32 -- Received: from localhost (smtp2.rz.uni-hohenheim.de [144.41.4.42]) 7, 32 -- by smtp1.rz.uni-hohenheim.de (Postfix) with ESMTP id 806DC11D401 7, 32 -- for {Microscopy-at-microscopy.com} ; Mon, 21 Jan 2008 21:43:58 +0100 (CET) 7, 32 -- X-Virus-Scanned: amavisd-new at uni-hohenheim.de 7, 32 -- Received: from smtp1.rz.uni-hohenheim.de ([144.41.4.41]) 7, 32 -- by localhost (webmail3.rz.uni-hohenheim.de [144.41.4.42]) (amavisd-new, port 10024) 7, 32 -- with ESMTP id UrTnPOubZH1G for {Microscopy-at-microscopy.com} ; 7, 32 -- Mon, 21 Jan 2008 21:43:52 +0100 (CET) 7, 32 -- Received: from webmail.uni-hohenheim.de (webmail2.rz.uni-hohenheim.de [144.41.4.31]) 7, 32 -- by smtp1.rz.uni-hohenheim.de (Postfix) with ESMTP id 4E35B11D3FB 7, 32 -- for {Microscopy-at-microscopy.com} ; Mon, 21 Jan 2008 21:43:51 +0100 (CET) 7, 32 -- Received: by webmail.uni-hohenheim.de (Postfix, from userid 48) 7, 32 -- id CA7B060153; Mon, 21 Jan 2008 21:43:51 +0100 (CET) 7, 32 -- Received: from RZMS-AB-F5-103.net.uni-hohenheim.de (RZMS-AB-F5-103.net.uni-hohenheim.de [144.41.103.45]) 7, 32 -- by webmail.uni-hohenheim.de (IMP) with HTTP 7, 32 -- for {heller-at-10.0.0.5} ; Mon, 21 Jan 2008 21:43:51 +0100 7, 32 -- Message-ID: {1200948231.47950407b2ce8-at-webmail.uni-hohenheim.de} 7, 32 -- Date: Mon, 21 Jan 2008 21:43:51 +0100 7, 32 -- From: heller-at-uni-hohenheim.de 7, 32 -- To: Microscopy-at-microscopy.com 7, 32 -- Subject: sectioning watermelon seeds 7, 32 -- References: {200801171628.m0HGSGiA017680-at-ns.microscopy.com} 7, 32 -- In-Reply-To: {200801171628.m0HGSGiA017680-at-ns.microscopy.com} 7, 32 -- MIME-Version: 1.0 7, 32 -- Content-Type: text/plain; charset=ISO-8859-1 7, 32 -- Content-Transfer-Encoding: 8bit 7, 32 -- User-Agent: Internet Messaging Program (IMP) 3.2.2 7, 32 -- X-Originating-IP: 144.41.103.45 ==============================End of - Headers==============================
I am not specialist in botanics, but my common sense tells me that perhaps you could cut the seed in 2 in the length, it would (1) make it thin enough for the fixative to efficiently penetrate (2) avoid the need to perforate the coat. If it is still too thick (for example the tissue next to the coat has artifacts), you could perhaps cut slices.
Regards,
Stephane
----- Original Message ---- X-from: "heller-at-uni-hohenheim.de" {heller-at-uni-hohenheim.de} To: nizets2-at-yahoo.com Sent: Monday, January 21, 2008 9:49:20 PM
Dear Beth,
I only have experience in sectioning seeds of Orobanche cumana. I was successful doing semi- and ultrathin sections after embedding in LR-White. The first prepartion step was to perforate the seeds with a fine needle. Without perforating the seed coat, it was impossible to get anything into these tiny seeds.
I think seeds are always diffcult, because their coat is quite tight, their water content extremely low, and they store masses of starch grains or other storage material which are difficult to fix and to section. Watermelon seeds are quite large. Maybe you can cut them and try to embed the pieces?
Good Luck, Anne
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi all, } Happy New Year! } Does anyone have experience thick-sectioning watermelon seeds? We're } working with mature seeds - black seed coat - unfixed, not embedded. } The seeds are left in a moist chamber overnight to imbibe water. } Vibratome sectioning didn't work well. Any suggestions? Would fixing } and embedding make them easier to section? } } I think they're really only good for spitting...but the researcher } doesn't want to hear that;-) } Any help would be greatly appreciated. } Many thanks, } Beth } } Beth Richardson } Plant Biology Department } University of Georgia } Athens, GA 30602 } 706-542-1790 } } } ==============================Original Headers============================== } 4, 19 -- From beth-at-plantbio.uga.edu Thu Jan 17 10:21:22 2008 } 4, 19 -- Received: from dogwood.plantbio.uga.edu (dogwood.plantbio.uga.edu } [128.192.26.2]) } 4, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } m0HGLLmX009715 } 4, 19 -- for {microscopy-at-microscopy.com} ; Thu, 17 Jan 2008 10:21:21 -0600 } 4, 19 -- Received: from [128.192.26.46] ([128.192.26.46]) } 4, 19 -- (authenticated user beth-at-plantbio.uga.edu) } 4, 19 -- by dogwood.plantbio.uga.edu } 4, 19 -- (using TLSv1/SSLv3 with cipher AES128-SHA (128 bits)) } 4, 19 -- for microscopy-at-microscopy.com; } 4, 19 -- Thu, 17 Jan 2008 11:21:15 -0500 } 4, 19 -- Message-Id: {80AFBBC9-F649-4422-84BA-331CE918C9EB-at-plantbio.uga.edu} } 4, 19 -- From: Beth Richardson {beth-at-plantbio.uga.edu} } 4, 19 -- To: microscopy microscopy {microscopy-at-microscopy.com} } 4, 19 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; } delsp=yes } 4, 19 -- Content-Transfer-Encoding: 7bit } 4, 19 -- Mime-Version: 1.0 (Apple Message framework v915) } 4, 19 -- Subject: sectioning watermelon seeds } 4, 19 -- Date: Thu, 17 Jan 2008 11:22:05 -0500 } 4, 19 -- X-Mailer: Apple Mail (2.915) } ==============================End of - Headers============================== }
==============================Original Headers============================== 7, 32 -- From heller-at-uni-hohenheim.de Mon Jan 21 14:44:01 2008 7, 32 -- Received: from smtp1.rz.uni-hohenheim.de (smtp1.rz.uni-hohenheim.de [144.41.4.41]) 7, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0LKi0pI030518 7, 32 -- for {Microscopy-at-microscopy.com} ; Mon, 21 Jan 2008 14:44:01 -0600 7, 32 -- Received: from localhost (smtp2.rz.uni-hohenheim.de [144.41.4.42]) 7, 32 -- by smtp1.rz.uni-hohenheim.de (Postfix) with ESMTP id 806DC11D401 7, 32 -- for {Microscopy-at-microscopy.com} ; Mon, 21 Jan 2008 21:43:58 +0100 (CET) 7, 32 -- X-Virus-Scanned: amavisd-new at uni-hohenheim.de 7, 32 -- Received: from smtp1.rz.uni-hohenheim.de ([144.41.4.41]) 7, 32 -- by localhost (webmail3.rz.uni-hohenheim.de [144.41.4.42]) (amavisd-new, port 10024) 7, 32 -- with ESMTP id UrTnPOubZH1G for {Microscopy-at-microscopy.com} ; 7, 32 -- Mon, 21 Jan 2008 21:43:52 +0100 (CET) 7, 32 -- Received: from webmail.uni-hohenheim.de (webmail2.rz.uni-hohenheim.de [144.41.4.31]) 7, 32 -- by smtp1.rz.uni-hohenheim.de (Postfix) with ESMTP id 4E35B11D3FB 7, 32 -- for {Microscopy-at-microscopy.com} ; Mon, 21 Jan 2008 21:43:51 +0100 (CET) 7, 32 -- Received: by webmail.uni-hohenheim.de (Postfix, from userid 48) 7, 32 -- id CA7B060153; Mon, 21 Jan 2008 21:43:51 +0100 (CET) 7, 32 -- Received: from RZMS-AB-F5-103.net.uni-hohenheim.de (RZMS-AB-F5-103.net.uni-hohenheim.de [144.41.103.45]) 7, 32 -- by webmail.uni-hohenheim.de (IMP) with HTTP 7, 32 -- for {heller-at-10.0.0.5} ; Mon, 21 Jan 2008 21:43:51 +0100 7, 32 -- Message-ID: {1200948231.47950407b2ce8-at-webmail.uni-hohenheim.de} 7, 32 -- Date: Mon, 21 Jan 2008 21:43:51 +0100 7, 32 -- From: heller-at-uni-hohenheim.de 7, 32 -- To: Microscopy-at-microscopy.com 7, 32 -- Subject: sectioning watermelon seeds 7, 32 -- References: {200801171628.m0HGSGiA017680-at-ns.microscopy.com} 7, 32 -- In-Reply-To: {200801171628.m0HGSGiA017680-at-ns.microscopy.com} 7, 32 -- MIME-Version: 1.0 7, 32 -- Content-Type: text/plain; charset=ISO-8859-1 7, 32 -- Content-Transfer-Encoding: 8bit 7, 32 -- User-Agent: Internet Messaging Program (IMP) 3.2.2 7, 32 -- X-Originating-IP: 144.41.103.45 ==============================End of - Headers==============================
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==============================Original Headers============================== 20, 20 -- From nizets2-at-yahoo.com Tue Jan 22 06:29:57 2008 20, 20 -- Received: from web37406.mail.mud.yahoo.com (web37406.mail.mud.yahoo.com [209.191.91.138]) 20, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m0MCTv0R011560 20, 20 -- for {microscopy-at-microscopy.com} ; Tue, 22 Jan 2008 06:29:57 -0600 20, 20 -- Received: (qmail 86931 invoked by uid 60001); 22 Jan 2008 12:29:57 -0000 20, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 20, 20 -- s=s1024; d=yahoo.com; 20, 20 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:Cc:MIME-Version:Content-Type:Message-ID; 20, 20 -- b=6oC041OI8SU/KOraTWvfimPYAwKv35IFKryfR1ute6M6+i42b9g/GMbJNgWRGLL2gzJ9t1TLRm4r7jjCAEYFQPDiynzhu7PSXEFHqRFrJcbiBnPy9wrDRRUcGCP4IgQUtnd6mSWczx968yt0lEufGukRpNTMlUdvfMCRZ+Fiobo=; 20, 20 -- X-YMail-OSG: 2_q8r1IVM1nQHcRer8i4DDUeJEFsvREdXU5uUCLixlw70swFy0fXkLginyQ_AEhoEfU034wft0tOn8DZKR4KnfTfRHqcHKtRhpexRhLbnMo6z5r9Sd6oWlTdW08CQTc73VtNd0jmL.ChOBM5odO_sz83l1MuH4aoqlsz3WdiqVHn 20, 20 -- Received: from [80.122.101.100] by web37406.mail.mud.yahoo.com via HTTP; Tue, 22 Jan 2008 04:29:56 PST 20, 20 -- X-Mailer: YahooMailRC/818.31 YahooMailWebService/0.7.160 20, 20 -- Date: Tue, 22 Jan 2008 04:29:56 -0800 (PST) 20, 20 -- From: Stephane Nizet {nizets2-at-yahoo.com} 20, 20 -- Subject: Re: [Microscopy] sectioning watermelon seeds 20, 20 -- To: heller-at-uni-hohenheim.de 20, 20 -- Cc: microscopy-at-microscopy.com 20, 20 -- MIME-Version: 1.0 20, 20 -- Content-Type: text/plain; charset=us-ascii 20, 20 -- Message-ID: {195330.86606.qm-at-web37406.mail.mud.yahoo.com} ==============================End of - Headers==============================
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Email: susan.trant-at-viha.ca Name: Susan Trant
Organization: Vancouver Island Health Authority
Title-Subject: [Filtered] Jeol 100CX-II
Question: Hello Everyone
We have purchased a new Jeol TEM microscope. We are asking anyone out there if they would like our old Jeol 100CX-II. The vendors have told us that they will crate the microscope up and then it is ours to dispose of. The lucky party must pay to have the microscope shipped to their location.
Sue Trant EM Technologist Vancouver Island Health Authority 1952 Bay Street Victoria BC V8R 1J8 250-370-8402
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Title-Subject: [Filtered] Project MICRO: New Sandbox Contact
Question: We are pleased to announce that the Microscopy Society of Americaís (MSA) collection of sand for use with Project MICRO Microscopic Explorations activity 6 is now housed at McCrone Associates, Inc. in Westmont , Illinois; and is under the direction of Heidi Ullberg. Mr. Joe Neilly has faithfully dispatched sand samples to educators all over the country for many years. Joe has enjoyed his duties as ëKeeper of the Sandboxí, and as he passes the shovel to Heidi says that he will miss visiting the far reaches of the world - one sand sample at a time.
To view an inventory of the collection visit MSAís website at:
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Email: holsen-at-awscorp.com Name: Harald Olsen
Organization: American World Services
Title-Subject: [Filtered] vacuum pump applications to SEM
Question: Hello,
I work at American World Services in Washington, D.C., we represent the French vacuum pump company Normetex. I was referred to this listserv by Jon Norenburg of the American Microscopical Society as a way to get an answer to a question I have concerning and application for a specific type of ultrahigh vacuum pump.
I have heard that SEM and TEM rely on vacuum pumps, I am trying to figure out if they are similar to the product we represent. Normetex's pumps have traditionally been used in nuclear applications, as they are perfectly clean and dry and thus suited to a clean room environment. Because of their ability to safely handle corrosive or inert gases, I was wondering if they might also have an application with electron microscopy.
In terms of specifications, Normetex produces pumps that range in size from 15 m3/h to 600 m3/h, and produce an ultimate vacuum of 45 mbar on smaller models and 8x10-2 mbar on the larger models.
I am trying to figure out if these pumps would be appropriate for SEM or TEM, as well as who might best be able to make use of them. I realize that this is a very specific question, but I trust that if any resource would have an answer, it would be the members of this listserv.
Thank you,
Harald Olsen Project Assistant American World Services Corp. 1247 Wisconsin Ave., NW, Suite 201 Washington, DC 20007 (t) +1.202.296.3523 (f) +1.202.333.0017 holsen-at-awscorp.com www.awscorp.com
Has anyone on the list had any experiences (Good or Bad) updating the refrigeration system on a water chiller from R-12 to a current refrigerant (i.e. R-134a, etc.)?
We have had a older R-12 Haskris chiller die, and are looking at the possibility of replacing the compressor and condenser (water cooled), blowing and flushing out the lines, and refilling with a current R-12 replacement. The question is the mineral oil in the system. The R- 12 will replace easily, but what about the residual mineral oil.
Five years ago we updated a chiller, replacing the condenser and compressor, but we used synthetic oil (instead of mineral oil) however we still had R-12 so that´s what we used. The system has run perfectly fine 24/7 for 5 years. This would seem to say oil incompatibility is not a significant issue.
O.k., I´m being cheap. If I had $6000 I´d buy a new water chiller and be done with it. But I don´t. I´m hoping for a lower cost solution that will get me some time. Any thoughts?
Yes, we have professional refrigeration folks working on it but they are not sure on the smaller systems.
(Disclaimer: I have no financial interests in any refrigeration company, and have been working with Haskris systems for 25 years and love them.)
Richard E. Edelmann, Ph.D. Electron Microscopy Facility Director 364 Pearson Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.emf.muohio.edu
==============================Original Headers============================== 12, 23 -- From edelmare-at-muohio.edu Wed Jan 23 07:23:00 2008 12, 23 -- Received: from mulnx12.mcs.muohio.edu (mulnx12.mcs.muohio.edu [134.53.6.67]) 12, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0NDMxco018731 12, 23 -- for {microscopy-at-Microscopy.com} ; Wed, 23 Jan 2008 07:23:00 -0600 12, 23 -- Received: from mulnx24.mcs.muohio.edu (mulnx24.mcs.muohio.edu [134.53.6.11]) 12, 23 -- by mulnx12.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id m0NDMxZT009539 12, 23 -- for {microscopy-at-Microscopy.com} ; Wed, 23 Jan 2008 08:22:59 -0500 12, 23 -- Received: from [192.168.1.23] ([134.53.14.105]) 12, 23 -- by mulnx24.mcs.muohio.edu (Switch-3.1.8/Switch-3.1.7) with ESMTP id m0NDMx0D029237 12, 23 -- for {microscopy-at-Microscopy.com} ; Wed, 23 Jan 2008 08:22:59 -0500 12, 23 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} 12, 23 -- To: microscopy-at-Microscopy.com 12, 23 -- Date: Wed, 23 Jan 2008 08:22:59 -0500 12, 23 -- MIME-Version: 1.0 12, 23 -- Subject: Refurbishing - Updating Water chillers 12, 23 -- Message-ID: {4796F963.15055.D82BBEC-at-edelmare.muohio.edu} 12, 23 -- Priority: normal 12, 23 -- X-mailer: Pegasus Mail for Windows (4.41) 12, 23 -- Content-type: text/plain; charset=ISO-8859-1 12, 23 -- Content-description: Mail message body 12, 23 -- X-Scanned-By: MIMEDefang 2.57 on 134.53.6.67 12, 23 -- Content-Transfer-Encoding: 8bit 12, 23 -- X-MIME-Autoconverted: from Quoted-printable to 8bit by ns.microscopy.com id m0NDMxco018731 ==============================End of - Headers==============================
Our highschool has a Cambridge S200 SEM that was donated to us by Motorola. They actually donated two, and after months of assembling the most trustworthy pieces together as one unit, the thing now powers up, the pumps work and the screen lights up. All I can see on the screen is snow. No moving of apertures, stage or console controls seem to affect what is seen on the screen or produces an image. I am reasonably sure that the Wallace unit is putting out all the high voltages, so the detector should be getting power. The detector was removed from the scope and tested with a light, and it does produce a signal under these circumstances. Connected back to the column though, I see no signal on my oscilloscope at the test point on the input board for the signal from the detector, suggesting it is not doing anything. Feeding a test signal into the microscope at this same test point, I can visualize the signal on the screen, so the video electronics are working.
The filament fail light goes out when I turn up the filament, so I assume electrons should be speedng down the column and hitting the specimen. To the best of my ability the filament is centered and aligned in the cap properly.
This seems to be the only remaining issue with the microscope, after solving numerous others, but it has me stumped. The detector apparantly works, but it isn't working! Any advice?
-Dr. Mike Brown Science Dept Chair AAEC_PV
==============================Original Headers============================== 8, 17 -- From mbrown-at-aaechighschools.com Wed Jan 23 13:05:04 2008 8, 17 -- Received: from smtpoutwbe08.prod.mesa1.secureserver.net (smtpoutwbe08.prod.mesa1.secureserver.net [208.109.78.210]) 8, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m0NJ54jp025976 8, 17 -- for {Microscopy-at-Microscopy.Com} ; Wed, 23 Jan 2008 13:05:04 -0600 8, 17 -- Received: (qmail 26874 invoked from network); 23 Jan 2008 19:05:03 -0000 8, 17 -- Received: from unknown (HELO gem-wbe24.prod.mesa1.secureserver.net) (64.202.189.227) 8, 17 -- by smtpoutwbe08.prod.mesa1.secureserver.net with SMTP; 23 Jan 2008 19:05:03 -0000 8, 17 -- Received: (qmail 15048 invoked by uid 99); 23 Jan 2008 19:05:03 -0000 8, 17 -- Date: Wed, 23 Jan 2008 12:05:03 -0700 8, 17 -- From: mbrown-at-aaechighschools.com 8, 17 -- Subject: Issues with a Cambridge S200 SEM 8, 17 -- To: Microscopy-at-Microscopy.Com 8, 17 -- Message-ID: {20080123120503.889a5134facffa13190a02200f773d01.cdade38cdc.wbe-at-email.secureserver.net} 8, 17 -- MIME-Version: 1.0 8, 17 -- Content-Type: TEXT/plain; CHARSET=US-ASCII 8, 17 -- User-Agent: Web-Based Email 4.12.20 8, 17 -- X-Originating-IP: 71.35.53.132 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mlibbee-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: mlibbee-at-gmail.com Name: Marissa
Title-Subject: [Filtered] Allied Epoxy Bond 110
Question: I recently found Allied's 2-part Epoxy Bond 110 in my lab but could not locate the mixing literature. I've emailed Allied but am rather impatient...Does anyone know the ratio of resin to hardener and the temperature necessary to cure the epoxy?
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.org/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both swaffordisjim-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: swaffordisjim-at-gmail.com Name: Jim Swafford
Organization: retired on Dinjim ranch
Title-Subject: [Filtered] MT1 Ultra microtome
Question: Greetings. I am wanting to purchase a particular model of ultra microtome, Sorvall MT1, which was very popular in the late 1950's through 1960's. I anticipate using this instrument at Pittsburg State University which is a small school, ~6,000 students, located in Southeastern Kansas. If anyone knows the location of one of these microtomes that could be sold or donated, please contact me.
For quite some time I have wondered why NUNC did not make a Permanox Petri Dish smaller than 60 x 15 mm. I have grown to really appreciate the advantages of using the Permanox over standard Polystyrene dishes for tissue cultured cells grown for TEM experiments since every cell line that I have tried adheres well to them, they can withstand chemicals like acetone and propylene oxide that dissolve the polystyrene and the embedded cells come away from the Permanox so easily and smoothly.
With the special cells and reagents that I am now using for TEM, it is a big waste to grow cells over such a large area when a 35 x 15 mm dish would do nicely. I do want a dish not a chambered slide.
I would like to know if there are others (you) 1. who would switch to a smaller dish if they would be made available. (this is my pick) 2. who would like to use both the 60mm and 35mm dishes 3. who would only use the 60mm dishes If I get a reasonable response I will contact the company with my results to back up my request that they consider making the smaller dishes.
Comments are welcome.
For the survey, it may be best to answer me "Off-ListServer" so as not to fill up the emails of other members. I will let the ListServer know the tally after the replies come in.
Thanks to all, Pat
Patricia Stranen Connelly Biologist, Electron Microscopy NHLBI Electron Microscopy Core National Institutes of Health 14 Service Road South Bldg. 14E Rm. 111B MSC 5570 Bethesda, MD 20892-5570 Phone 301-496-3491 FAX 301-480-6560 connellyps-at-mail.nih.gov
==============================Original Headers============================== 10, 22 -- From connellyps-at-nhlbi.nih.gov Wed Jan 23 17:57:23 2008 10, 22 -- Received: from NIHCESSMTP3.hub.nih.gov (nihcessmtp3.hub.nih.gov [128.231.90.117]) 10, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0NNvNkj008142 10, 22 -- for {microscopy-at-microscopy.com} ; Wed, 23 Jan 2008 17:57:23 -0600 10, 22 -- Received: from NIHCESMLBX6.nih.gov ([156.40.71.206]) by NIHCESSMTP3.hub.nih.gov with Microsoft SMTPSVC(6.0.3790.1830); 10, 22 -- Wed, 23 Jan 2008 18:57:18 -0500 10, 22 -- Received: from 156.40.71.188 ([156.40.71.188]) by NIHCESMLBX6.nih.gov ([156.40.71.206]) via Exchange Front-End Server mail.nih.gov ([156.40.71.167]) with Microsoft Exchange Server HTTP-DAV ; 10, 22 -- Wed, 23 Jan 2008 23:57:18 +0000 10, 22 -- User-Agent: Microsoft-Entourage/11.3.6.070618 10, 22 -- Date: Wed, 23 Jan 2008 18:54:47 -0500 10, 22 -- Subject: Interest in smaller "Permanox" dishes? 10, 22 -- From: Patricia Connelly {connellyps-at-nhlbi.nih.gov} 10, 22 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 10, 22 -- Message-ID: {C3BD3DF7.13F0%connellyps-at-nhlbi.nih.gov} 10, 22 -- Thread-Topic: Interest in smaller "Permanox" dishes? 10, 22 -- Thread-Index: AcheG1Vmk7YdFMoOEdyXCwANk2Yv1A== 10, 22 -- Mime-version: 1.0 10, 22 -- Content-type: text/plain; 10, 22 -- charset="ISO-8859-1" 10, 22 -- X-OriginalArrivalTime: 23 Jan 2008 23:57:18.0661 (UTC) FILETIME=[AFCC9F50:01C85E1B] 10, 22 -- Content-Transfer-Encoding: 8bit 10, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m0NNvNkj008142 ==============================End of - Headers==============================
I have a user who was trying to use a camera without microscope to image something that he could not get on the stage of the microscope. In playing around, he discovered that Nikon objectives actually have a C-mount compatible thread. When he screwed a 2cm extender and then a 20x objective onto the CCD camera, he was able to image a specimen at a distance of about 2cm. Naively, I would have said that an infinity corrected lens should not form an image in the plane of the CCD chip, but obviously, the specimen is not at the appropriate focal point of the lens (~2mm). Can someone explain why this works? Thanks- Dave
Dr. David Knecht Department of Molecular and Cell Biology Co-head Flow Cytometry and Confocal Microscopy Facility U-3125 91 N. Eagleville Rd. University of Connecticut Storrs, CT 06269 860-486-2200 860-486-4331 (fax)
==============================Original Headers============================== 4, 19 -- From david.knecht-at-uconn.edu Thu Jan 24 08:19:42 2008 4, 19 -- Received: from mail1.uits.uconn.edu (mail1.uits.uconn.edu [137.99.25.203]) 4, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0OEJgCb025204 4, 19 -- for {microscopy-at-microscopy.com} ; Thu, 24 Jan 2008 08:19:42 -0600 4, 19 -- Received: from d46h174.public.uconn.edu (d46h174.public.uconn.edu [137.99.46.174]) 4, 19 -- by mail1.uits.uconn.edu (8.13.6/8.11.6) with ESMTP id m0OEJY9H005844 4, 19 -- for {microscopy-at-microscopy.com} ; Thu, 24 Jan 2008 09:19:34 -0500 4, 19 -- Message-Id: {D27EFCEF-F4C3-469F-9367-6E949899AAB5-at-uconn.edu} 4, 19 -- From: David Knecht {david.knecht-at-uconn.edu} 4, 19 -- To: microscopy microscopy {microscopy-at-microscopy.com} 4, 19 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 4, 19 -- Content-Transfer-Encoding: 7bit 4, 19 -- Mime-Version: 1.0 (Apple Message framework v915) 4, 19 -- Subject: Infinity corrected objectives 4, 19 -- Date: Thu, 24 Jan 2008 09:19:34 -0500 4, 19 -- X-Mailer: Apple Mail (2.915) 4, 19 -- X-UConn-MailScanner-Information: Contact UConn Help Desk 860-486-4357 for more information. 4, 19 -- X-UConn-MailScanner: Found to be clean 4, 19 -- X-UConn-MailScanner-SpamCheck: ==============================End of - Headers==============================
Mike, Are you getting emission current? Does it (emission current) appear to behave properly as you heat the filament? If not, then there is some kind of problem with the gun circuit. If so, I'd say the beam isn't making it down the column. Are the various beam-steering circuits behaving properly? Have you tried pulling out all the apertures to try and get some response? Are both the upper and lower scan coils functioning properly? Any of the coils have the potential to drive the beam off to the side somewhere and make it disappear.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: mbrown-at-aaechighschools.com [mailto:mbrown-at-aaechighschools.com] Sent: Wednesday, January 23, 2008 2:08 PM To: kenconverse-at-qualityimages.biz
Our highschool has a Cambridge S200 SEM that was donated to us by Motorola. They actually donated two, and after months of assembling the most trustworthy pieces together as one unit, the thing now powers up, the pumps work and the screen lights up. All I can see on the screen is snow. No moving of apertures, stage or console controls seem to affect what is seen on the screen or produces an image. I am reasonably sure that the Wallace unit is putting out all the high voltages, so the detector should be getting power. The detector was removed from the scope and tested with a light, and it does produce a signal under these circumstances. Connected back to the column though, I see no signal on my oscilloscope at the test point on the input board for the signal from the detector, suggesting it is not doing anything. Feeding a test signal into the microscope at this same test point, I can visualize the signal on the screen, so the video electronics are working.
The filament fail light goes out when I turn up the filament, so I assume electrons should be speedng down the column and hitting the specimen. To the best of my ability the filament is centered and aligned in the cap properly.
This seems to be the only remaining issue with the microscope, after solving numerous others, but it has me stumped. The detector apparantly works, but it isn't working! Any advice?
-Dr. Mike Brown Science Dept Chair AAEC_PV
==============================Original Headers============================== 8, 17 -- From mbrown-at-aaechighschools.com Wed Jan 23 13:05:04 2008 8, 17 -- Received: from smtpoutwbe08.prod.mesa1.secureserver.net (smtpoutwbe08.prod.mesa1.secureserver.net [208.109.78.210]) 8, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m0NJ54jp025976 8, 17 -- for {Microscopy-at-Microscopy.Com} ; Wed, 23 Jan 2008 13:05:04 -0600 8, 17 -- Received: (qmail 26874 invoked from network); 23 Jan 2008 19:05:03 -0000 8, 17 -- Received: from unknown (HELO gem-wbe24.prod.mesa1.secureserver.net) (64.202.189.227) 8, 17 -- by smtpoutwbe08.prod.mesa1.secureserver.net with SMTP; 23 Jan 2008 19:05:03 -0000 8, 17 -- Received: (qmail 15048 invoked by uid 99); 23 Jan 2008 19:05:03 -0000 8, 17 -- Date: Wed, 23 Jan 2008 12:05:03 -0700 8, 17 -- From: mbrown-at-aaechighschools.com 8, 17 -- Subject: Issues with a Cambridge S200 SEM 8, 17 -- To: Microscopy-at-Microscopy.Com 8, 17 -- Message-ID: {20080123120503.889a5134facffa13190a02200f773d01.cdade38cdc.wbe-at-email.secure server.net} 8, 17 -- MIME-Version: 1.0 8, 17 -- Content-Type: TEXT/plain; CHARSET=US-ASCII 8, 17 -- User-Agent: Web-Based Email 4.12.20 8, 17 -- X-Originating-IP: 71.35.53.132 ==============================End of - Headers==============================
==============================Original Headers============================== 23, 27 -- From kenconverse-at-qualityimages.biz Thu Jan 24 10:29:15 2008 23, 27 -- Received: from dpmailmta05.doteasy.com (dpmailmta05-20.doteasy.com [65.61.218.100]) 23, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0OGTFIq010206 23, 27 -- for {microscopy-at-msa.microscopy.com} ; Thu, 24 Jan 2008 10:29:15 -0600 23, 27 -- Received: from dpmail16.doteasy.com (unverified [192.168.101.16]) 23, 27 -- by dpmailmta05.doteasy.com (DEO) with ESMTP id 24373207-1814644 23, 27 -- for multiple; Thu, 24 Jan 2008 08:30:05 -0800 23, 27 -- Received: from cpe-72-227-100-25.maine.res.rr.com [72.227.100.25] by dpmail16.doteasy.com with SMTP; 23, 27 -- Thu, 24 Jan 2008 08:28:56 -0800 23, 27 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 23, 27 -- To: {mbrown-at-aaechighschools.com} , 23, 27 -- "MSA Listserver" {Microscopy-at-msa.microscopy.com} 23, 27 -- Subject: RE: [Microscopy] Issues with a Cambridge S200 SEM 23, 27 -- Date: Thu, 24 Jan 2008 11:28:46 -0500 23, 27 -- Message-ID: {000e01c85ea6$326e9870$6401a8c0-at-Ken} 23, 27 -- MIME-Version: 1.0 23, 27 -- Content-Type: text/plain; 23, 27 -- charset="us-ascii" 23, 27 -- X-Priority: 3 (Normal) 23, 27 -- X-MSMail-Priority: Normal 23, 27 -- X-Mailer: Microsoft Outlook, Build 10.0.6822 23, 27 -- Thread-Index: Achd81aEYK6eZdxhSSmTrIW/5AYgLAAsa/Ig 23, 27 -- In-Reply-To: {200801231908.m0NJ8Qmw030307-at-ns.microscopy.com} 23, 27 -- Importance: Normal 23, 27 -- X-MIMEOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 23, 27 -- Content-Transfer-Encoding: 8bit 23, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m0OGTFIq010206 ==============================End of - Headers==============================
Hi All - I would appreciate hearing from users of environmental SEMs, specifically as to imaging live cells. This is a new area for me, as my expertise is in TEM, so I am trying to gather as much information as possible.
We already have a variable pressure SEM, which is not ideal for viewing cells, although to my knowledge we have never tried it. I have been looking at manufacturers of ESEMs, and have found the Zeiss EVO LS and the FEI Quanta. Any user opinions on these two systems are welcome as well.
Thanks, Jessica ____________________ Jessica Cervantes Bend Research Inc Bend, OR 97701 www.bendres.com
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==============================Original Headers============================== 6, 14 -- From cervantes-at-bendres.com Thu Jan 24 10:53:47 2008 6, 14 -- Received: from mail.bendres.com (mail.bendres.com [216.228.161.112] (may be forged)) 6, 14 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0OGrl0m023702 6, 14 -- for {Microscopy-at-microscopy.com} ; Thu, 24 Jan 2008 10:53:47 -0600 6, 14 -- MIME-Version: 1.0 6, 14 -- Content-Type: text/plain; 6, 14 -- charset="iso-8859-1" 6, 14 -- Subject: ESEM Imaging of Live Cells 6, 14 -- Date: Thu, 24 Jan 2008 08:53:47 -0800 6, 14 -- Message-ID: {8943D65F9AD70E4488AD6DE09F150887EEE5E9-at-BRIEX04A} 6, 14 -- From: "Cervantes, Jessica" {cervantes-at-bendres.com} 6, 14 -- To: {Microscopy-at-microscopy.com} 6, 14 -- Content-Transfer-Encoding: 8bit 6, 14 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m0OGrl0m023702 ==============================End of - Headers==============================
The answer is quite simple. For the sake of simplicity, a compound microscope has two key "imaging" lenses: the objective and the eyepiece. Optically, the job of the eyepiece is to create a (usually) magnified image at the appropriate location to act as a "specimen" for the eyepiece. In order for there to be an image, the light carrying the original specimen information must convege to a point of focus. There are two ways to achieve that result: a. To place the object just beyond the front focal plane of the objective (FFPo), resulting in a real image at some fixed distance b. To place the object exactly at the FFPo, sending the imaging information up through the optical train in a bundle of rays which is either parallel to the optic axis (on-axis info) or some principle ray at some angle to that axis (off-axis info). Note that if these rays are parallel, they cannot converge to form an image. We say that the information "goes to inifinity", hence never forms an image. In this case, you need a second lens (the telan or tube lense) to create the necessary convergence at the right location for the eyepiece. The space between the back focal plane of the objective and the tube lens is what is known as infinity space, a design which gives considerable freedom to microscope designers.
So, if you take a lens that was meant to work in Condition b and move the object slightly further away from the front of the lens, you will change the optics to Condition a. This is another one of those cases, like NA, where what is written on the objective is only true when the microscope is properly set up and aligned for Koehler illumination.
Hope this was helpful!
Best regards, Barbara Foster, President
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Thank you to the dozens of you who sent in suggestions. Most suggestions were that electrons were not making it down the column to the specimen. I removed one of the apertures. Electrons are now getting down the column to the specimen when I dial in the place where the aperture used to be. When I turn up the filament until the fail light goes off, the screen flashes, and the nature of the snow on the screen changes. I can visualize vertical stripes down the screen. These move around to the left and right when I move the specimen. Rotating the image moves the lines also, but they always stay vertical. In fact rotating the specimen also moves the vertical lines but does not make them horizontal.
What is the issue here?
-Dr. Mike Brown Science Dept Chair AAEC_PV
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This Question was submitted to Ask-A-Microscopist by (stephen.ruiz-at-siemens.com) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Thursday, January 24, 2008 at 14:41:03 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both stephen.ruiz-at-siemens.com as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Email: stephen.ruiz-at-siemens.com Name: Stephen Ruiz
Organization: Siemens DX
Education: Graduate College
Location: Norwood, Ma., USA
Title: Digital SLR Camera
Question: Any suggestions on a good SLR digital camera for both micro and macro images?
David sent me a follow-up to this posting, asking how to incorporate all of this info into teaching microscopy. I know that a number of you teach and might find the infomration useful, so here's a copy of my answer to him.
Dear David,
There is a really neat way to do all of this. It starts with a simple experiment with a hand lens. a. Difference between object and image Using a simple hand lens, have the students look through the lens at their finger nails (be prepared for lots of silly groans!). First have them put their finger close to the lens, then have them slowly move it back. As they do, the image of their finger will become larger and larger. At some point it will disappear. Then, if they keep watching carefully, the image will reappear, inverted. Then, have them remove the lens and look directly at their finger. At this point I tell the, "Notice that at no point did your finger leave your hand." This really solidifies the concept of object (their finger) and image (what they saw through the lens, after the lens has operated on that information. Take home message: our job as microscopists is to capture in the image, with as much fidelity as possible, the information from the object. Second take home messages: Lenses can lie.
b. Find the focal length of the lens Using a simple, single hand lens, have the students find the focus the image of the overhead lights on the table in front of them. The distance from the physical center of the lens to the table top is the focal length. This set up the following concepts: Focal length Focal plane Front focal plane
c. Four cases of lens Now that they understand the concept of object/image and focal length, you can repeat Experiment A to illustrate the case of the object (1) inside the focal length (forms virtual, upright image on same side of the lens as the object (2) at the focal length (informrtion goes to infinity: no convergence of date; No image) (3) slightly beyond the focal length (real image, on other side of the lens; magnification determined by distance of object from lens) (4) a great distance beyond the focal length (light coming from "infinity"; rays form bundle which is parallel to either optic axis or principle ray through optical axis). You can reinforce all of these using simple ray diagrams found in any high school physics book.
d. All of this sets up the discussion for (1) Infinity vs. fixed tube length optics and why you just can't willy nilly change objectives from stands of one design to stands of the other (2) Spherical and chromatic aberration (3) Which then leads to discussions of different types of glassware on the microscope and how to make educated buying decisions based on corrections, working distances.
It's a great set of lecture/demonstrations that really carries through to discussions of NA, resolution versus detection, and contrast techniques...a little bit of physics that goes a long way. And because they are doing demonstrations throughout the lecture, they stay involved AND tend to remember it all (every teachers' dream).
At this point, I'd recommend getting a copy of my book... it's all in there... but we have just come to the end of the supply. I need to talk to Zeiss, to see if they are interested in updating and reprinting. My other challenge is finding the time to do that. I do have some lecture notes that I use when I teach, but they are primarily just the diagrams, etc.
Hope this was helpful.
Best regards, Barbara
At 04:11 PM 1/24/2008, you wrote: } THanks for the explanation. It makes sense and the object(ive) lesson is especially important (abberations aside). I will have to think how I can incorporate this into my microscopy course. Dave } } On Jan 24, 2008, at 2:17 PM, {mailto:bfostermme-at-sbcglobal.net} bfostermme-at-sbcglobal.net wrote: } } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
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Dear Group - what is your opinion for a TEM bottom mounted camera for the best resolution for high mag - camera with lens or fiber optic? Appreciate any comments. Thanks Barbara
==============================Original Headers============================== 1, 18 -- From maloneyb-at-fiu.edu Fri Jan 25 05:14:12 2008 1, 18 -- Received: from fmailhost06.isp.att.net (fmailhost06.isp.att.net [204.127.217.106]) 1, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0PBECAj022401 1, 18 -- for {Microscopy-at-microscopy.com} ; Fri, 25 Jan 2008 05:14:12 -0600 1, 18 -- Received: from [192.168.1.2] (adsl-074-166-189-137.sip.mia.bellsouth.net[74.166.189.137]) 1, 18 -- by isp.att.net (frfwmhc06) with ESMTP 1, 18 -- id {20080125111411H0600colrte} ; Fri, 25 Jan 2008 11:14:11 +0000 1, 18 -- X-Originating-IP: [74.166.189.137] 1, 18 -- Message-ID: {4799C431.5010803-at-fiu.edu} 1, 18 -- Date: Fri, 25 Jan 2008 06:12:49 -0500 1, 18 -- From: barbara maloney {maloneyb-at-fiu.edu} 1, 18 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.7.2) Gecko/20040804 Netscape/7.2 (ax) 1, 18 -- X-Accept-Language: en-us, en 1, 18 -- MIME-Version: 1.0 1, 18 -- To: Microscopy-at-microscopy.com 1, 18 -- Subject: digital camera with lens vs. fiber optic coupled camera 1, 18 -- Content-Type: text/plain; charset=us-ascii; format=flowed 1, 18 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
only fiber optic coupling - as far as I can tell. best regards Reinhard Rachel --
---------------------- PD Dr. Reinhard Rachel Universitaet Regensburg Centre for EM - NWF III - -at-Institute for Anatomy Universitaetsstr. 31 D-93053 Regensburg - Germany tel +49 941 943 2837, 1720 fax +49 941 943 2868 mail reinhard.rachel-at-biologie.uni-r.de office: VKL 3.1.29
==============================Original Headers============================== 5, 25 -- From reinhard.rachel-at-biologie.uni-regensburg.de Fri Jan 25 07:23:23 2008 5, 25 -- Received: from rrzmta2.rz.uni-regensburg.de (rrzmta2.rz.uni-regensburg.de [194.94.155.53]) 5, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0PDNMpS006661 5, 25 -- for {Microscopy-at-Microscopy.Com} ; Fri, 25 Jan 2008 07:23:22 -0600 5, 25 -- Received: from rrzmta2.rz.uni-regensburg.de (localhost [127.0.0.1]) 5, 25 -- by localhost (Postfix) with SMTP id C6CE5552FB 5, 25 -- for {Microscopy-at-Microscopy.Com} ; Fri, 25 Jan 2008 14:23:26 +0100 (CET) 5, 25 -- Received: from gwsmtp1.uni-regensburg.de (gwsmtp1.rz.uni-regensburg.de [132.199.4.80]) 5, 25 -- by rrzmta2.rz.uni-regensburg.de (Postfix) with ESMTP id C0F58552E8 5, 25 -- for {Microscopy-at-Microscopy.Com} ; Fri, 25 Jan 2008 14:23:26 +0100 (CET) 5, 25 -- Received: from uni-regensburg-MTA by gwsmtp1.uni-regensburg.de 5, 25 -- with Novell_GroupWise; Fri, 25 Jan 2008 14:23:21 +0100 5, 25 -- Message-Id: {4799F0D4.044C.0054.0-at-biologie.uni-regensburg.de} 5, 25 -- X-Mailer: Novell GroupWise Internet Agent 7.0.2 HP 5, 25 -- Date: Fri, 25 Jan 2008 14:23:16 +0100 5, 25 -- From: "reinhard rachel" {reinhard.rachel-at-biologie.uni-regensburg.de} 5, 25 -- To: {Microscopy-at-Microscopy.Com} 5, 25 -- Subject: [Microscopy] digital camera 5, 25 -- References: {200801251114.m0PBEYRH022815-at-ns.microscopy.com} 5, 25 -- In-Reply-To: {200801251114.m0PBEYRH022815-at-ns.microscopy.com} 5, 25 -- Mime-Version: 1.0 5, 25 -- Content-Type: text/plain; charset=US-ASCII 5, 25 -- Content-Disposition: inline 5, 25 -- Content-Transfer-Encoding: 8bit 5, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m0PDNMpS006661 ==============================End of - Headers==============================
Sales and Sales Support Positions Available in the USA
Position #1 – Central and South Eastern Region Sales Representative US / Canada
Bitplane is looking for a biologist with strong computer skills and at least one year of hands-on experience using a confocal or similar 3D advanced light microscope.
Feel free to forward this e-mail if know someone in your facility who would be interested.
The duties of this position include:
• Customer visits and analysis of customer's imaging needs. • Demonstration of the software and onsite work with the customer • Organization of exhibitions and workshops. • Sales support of existing customers. The candidate is expected to have an outgoing personality with strong communication skills and should look forward to increased responsibility. At least 50% travel will be required. Representative is required to live in the territory they cover. Benefits include a base salary, performance based commission, 401K plan, healthcare, and vacation. Representative will work out of a home office. We offer a team of 18 people, fun to work with, and a truly international environment that provides the resources required to grow. We don't mind if the candidate does not have much business experience and we are prepared to show him/her the sales skills at the job.
Position #2 – Sales Support Manager
Bitplane is looking for a candidate with a science background and knowledge of the confocal and 3D microscope community.
Feel free to forward this e-mail if know someone in your facility who would be interested.
The duties of this position include:
• Identifying potential new regional customers via web searches, literature searches, marketing campaigns, trade shows, and customer referrals. • Introduction of Bitplane products and services to potential customers via email, phone, and Webex. • Understanding potential customers needs related to products offered by Bitplane. • Organizing and planning workshops and demonstrations for regional sales representatives
The candidate is expected to have an outgoing personality with strong communication skills and should look forward to increased responsibility. Travel not required. Benefits include a base salary, 401K plan, healthcare, and vacation. Representative will work out of a home office. We offer a team of 18 people, fun to work with, and a truly international environment that provides the resources required to grow.
Bitplane is an international company specializing in the sale of software for the visualization and analysis of 3D and 4D microscope images. More information can be found at www.bitplane.com
Interested persons should respond directly to Michael C. Wussow (mike-at-bitplane.com 651-336-4600) indicating which position they are interested in and providing a copy of their CV.
Bitplane Inc. Michael C. Wussow Vice President and General Manager Bitplane Inc.
Cell Phone: 651-336-4600 Fax: 866-691-9112 Toll Free: 1-888-3D-BITPX (332-4879) Visit Our Web Site At: www.bitplane.com
==============================Original Headers============================== 19, 32 -- From mike-at-bitplane.com Fri Jan 25 09:11:08 2008 19, 32 -- Received: from host55a.simplicato.com (host55a.simplicato.com [207.99.47.55]) 19, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0PFB85w023696 19, 32 -- for {Microscopy-at-microscopy.com} ; Fri, 25 Jan 2008 09:11:08 -0600 19, 32 -- Received: from localhost (localhost.simplicato.com [127.0.0.1]) 19, 32 -- by host55a.simplicato.com (Postfix) with ESMTP id 20D48B23581 19, 32 -- for {Microscopy-at-microscopy.com} ; Fri, 25 Jan 2008 10:11:08 -0500 (EST) 19, 32 -- Received: from host55a.simplicato.com ([127.0.0.1]) 19, 32 -- by localhost (host55a.simplicato.com [127.0.0.1]) (amavisd-new, port 10024) 19, 32 -- with ESMTP id 49479-02 for {Microscopy-at-microscopy.com} ; 19, 32 -- Fri, 25 Jan 2008 10:11:07 -0500 (EST) 19, 32 -- Received: from MikeLaptop (71-34-18-33.mpls.qwest.net [71.34.18.33]) 19, 32 -- by host55a.simplicato.com (Postfix) with ESMTP id 8B15AB234F1 19, 32 -- for {Microscopy-at-microscopy.com} ; Fri, 25 Jan 2008 10:11:07 -0500 (EST) 19, 32 -- Reply-To: {mike-at-bitplane.com} 19, 32 -- From: "Michael C. Wussow" {mike-at-bitplane.com} 19, 32 -- To: {Microscopy-at-microscopy.com} 19, 32 -- Subject: Job Posting 19, 32 -- Date: Fri, 25 Jan 2008 09:10:58 -0600 19, 32 -- Organization: Bitplane Inc. 19, 32 -- Message-ID: {030e01c85f64$7d911e90$78b35bb0$-at-com} 19, 32 -- MIME-Version: 1.0 19, 32 -- Content-Type: text/plain; 19, 32 -- charset="iso-8859-1" 19, 32 -- X-Mailer: Microsoft Office Outlook 12.0 19, 32 -- Thread-Index: AchfZDp2miYtgTJARIKUoKPxVIvtaw== 19, 32 -- Content-Language: en-us 19, 32 -- x-cr-hashedpuzzle: CMEP C/eL EBo8 EIwB FBH9 Gi0j H4KJ H91A IJTk IVtl JQmz JuZ1 Koc5 K8MC LPso LmMF;1;bQBpAGMAcgBvAHMAYwBvAHAAeQBAAG0AaQBjAHIAbwBzAGMAbwBwAHkALgBjAG8AbQA=;Sosha1_v1;7;{8504F353-188F-48D3-95C7-D470356798A6};bQBpAGsAZQBAAGIAaQB0AHAAbABhAG4AZQAuAGMAbwBtAA==;Fri, 25 Jan 2008 15:10:55 GMT;SgBvAGIAIABQAG8AcwB0AGkAbgBnAA== 19, 32 -- x-cr-puzzleid: {8504F353-188F-48D3-95C7-D470356798A6} 19, 32 -- X-Virus-Scanned: by amavisd-new at simplicato.com 19, 32 -- Content-Transfer-Encoding: 8bit 19, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m0PFB85w023696 ==============================End of - Headers==============================
We're having some beam instability problems with our tool. The local fixit person would like to rule out the high voltage as the cause. He asked me to find an HV tank to substitute. Is it possible someone has a tank (or other parts) sitting around, gathering dust?
Perhaps someone has had this problem before. After turning on the beam, the current drops. It wavers and then comes back only to repeat after a short time. The time periods are approximate; 20 minutes at first, and 10 minutes in the repeat cycle. If the beam is turned off for several minutes while the HV is ramped up, there will be a longer time period before the current drops again.
TIA, Annie --
+++++++++++++++++++++++++++++
R. Ann Bliss, Technologist Chemistry Materials, Earth and Life Sciences Materials Science and Technology Division Lawrence Livermore National Laboratory
_____________________________
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This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both andrea-at-ncmir.ucsd.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: andrea-at-ncmir.ucsd.edu Name: Andrea Thor
Organization: UCSD
Title-Subject: [Filtered] problems cutting serial thick sections (above 1-2 microns) without getting serious pitting of the blockface
Question: I am having some problems in cutting serial thick sections (above 1-2 microns) without getting serious pitting of the blockface. I heard this is not uncommon when pushing the section thickness to the extreme (such as 3-5 microns). We usually deal with the situation by refacing the block after each thick section, but this of course would make true "serial" sectioning impossible. The blocks I am working with are either cardic left ventricle or striatal tissue embedded in Durcupan.
If anyone has tried or has a protocol or techniques, I'd be very grateful to hear about them. It could save us tons of time and frustration as we develop a new set of protocols.
This Question was submitted to Ask-A-Microscopist by (davefissell-at-yahoo.com) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Saturday, January 26, 2008 at 04:33:33 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both davefissell-at-yahoo.com as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Email: davefissell-at-yahoo.com Name: David Fissell
Organization: CSU-DH
Education: Graduate College
Location: Brownsburg, IN, USA
Title: Microscope Ergonomics
Question: Visual inspection with a microscope is a common work situation for many industries. It is believed, poor ergonomic design of workstations where work demands involving a microscope are high (4-6 hours/work day) result in substandard performance. My question: Are you familiar with any research/industry guidelines supporting or countering this believe? Do you know of any ergonomic guidelines/standards prescribed for microscopist workstation design? Thank You David PS: My specific area of research is the impact on visual perception in industrial behavioral situations.
I am not sure of any specific health and safety regulations issued by any any safety organizations.
But I would suggest that appropriate or similar regulations such as would be useful: 1. The provision, use and maintenance of workplace equipment (PUWER 1998 UK safety regulations) 2. Display Screen Equipment (computers and similar instruments) regulations (DSE 2002 UK safety regulations) 3. and specific safety handling regulations for specimens such as chemical, biological and microbiological where appropriate in the lab.
I did a quick Google search (see below) and found a few industry and university websites. They all seem to consider ergonomics, optimal operation, good maintenance, environment, work activity patterns and nature of the specimen and any chemicals as most important. Most users seem to prefer binocular eyepieces with a good range of easy to use adjustments (eg dioptre correction and interocular distance.
I hope this helps and sorry about all the UK safety references but I'm sure there will be similar US regulations.
Good luck
Malcolm
Malcolm Haswell e.m. unit Chemispec School of Health, Natural and Social Sciences Fleming Building University of Sunderland Tyne & Wear SR1 3SD UK e-mail: malcolm.haswell-at-sunderland.ac.uk
----- Original Message ----- X-from: davefissell-at-yahoo.com
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Email: z.zhou-at-sheffield.ac.uk Name: Zoe Zhou
Organization: The University of Sheffield
Title-Subject: [Filtered] FIB
Question: I'm using a FEI quanta 200 3D focused ion beam microscope. I find tricky to control and understand the carbon deposition quality. I wonder what the gas injection process is while doing either C or Pt deposition pads. Is it an electron or ion plasma enhanced chemical vapour deposition process? Can anybody lead me to some related literatures?
David, This particular information is considered more critical in the area of Patholgy, either in pharmaceutical or hospital settings (or associated contract work). Some pathologists are expected to spend 40 hours a week doing nothing but reviewing thousands of microscope slides. It is one case where ergonomic statistics have definitely been researched extensively, since improving efficiency by a mere 5% can have quite an impact.
I personally do not have a source for this information, but a pathology oriented website probably could help.
Good luck, ~Gregg
Gregg Sobocinski Imaging Specialist/Microscopist University of Michigan, MCDB Dept. Ann Arbor, Michigan USA
-----Original Message----- X-from: davefissell-at-yahoo.com [mailto:davefissell-at-yahoo.com] Sent: Saturday, January 26, 2008 10:57 AM To: Sobocinski, Gregg
This Question was submitted to Ask-A-Microscopist by (davefissell-at-yahoo.com) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Saturday, January 26, 2008 at 04:33:33 Remember to consider the Grade/Age of the student when considering the Question ------------------------------------------------------------------------ --- Please reply to both davefissell-at-yahoo.com as well as to the Microscopy Listserver ------------------------------------------------------------------------ ---
Email: davefissell-at-yahoo.com Name: David Fissell
Organization: CSU-DH
Education: Graduate College
Location: Brownsburg, IN, USA
Title: Microscope Ergonomics
Question: Visual inspection with a microscope is a common work situation for many industries. It is believed, poor ergonomic design of workstations where work demands involving a microscope are high (4-6 hours/work day) result in substandard performance. My question: Are you familiar with any research/industry guidelines supporting or countering this believe? Do you know of any ergonomic guidelines/standards prescribed for microscopist workstation design? Thank You David PS: My specific area of research is the impact on visual perception in industrial behavioral situations.
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (agata.sena-at-hotmail.com) from http://microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Monday, January 28, 2008 at 05:24:58 ---------------------------------------------------------------------------
Email: agata.sena-at-hotmail.com Name: Agata
Organization: Materials Division
Education: Graduate College
Location: Brazil
Question: Dear all:
I'm characterizing gold tips by SEM/EDS. We are measuring the end of the tips and some of them have a deposit. I observed Si, S, Cu and Zn as contaminant in the EDS spectra of the deposit. The measurements are made on a FEI-Nano Lab and EDAX equipments. I didn't see any carbon. Is it possible thta these impurities be coming from SEM chamber by e-beam exposion?
I finally got sick of the noise and heat being thrown off by my water chiller system for the SEM, so I decided to move it to another room. Before I do this, though, the water lines have to be run up into the ceiling, over about 20 feet, then down to the microscope again. I've taken into consideration the connection end and plumbed in a valve system so I have a cut-off to run water through the chiller and piping without circulating it through the SEM, but I'm still wondering about the lifting power of the pump. Does the power required to lift the water lower the flow rate, or does the drop on the other end create enough of a siphon effect to cancel out any effects of the lift?
I seem to be able to reason this one out either way, depending on which outcome I'm looking for...
--Justin A. Kraft
==============================Original Headers============================== 3, 27 -- From kraftpiano-at-gmail.com Mon Jan 28 08:27:32 2008 3, 27 -- Received: from rv-out-0910.google.com (rv-out-0910.google.com [209.85.198.186]) 3, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0SERWxS027504 3, 27 -- for {microscopy-at-microscopy.com} ; Mon, 28 Jan 2008 08:27:32 -0600 3, 27 -- Received: by rv-out-0910.google.com with SMTP id k20so1635869rvb.30 3, 27 -- for {microscopy-at-microscopy.com} ; Mon, 28 Jan 2008 06:27:29 -0800 (PST) 3, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 3, 27 -- d=gmail.com; s=gamma; 3, 27 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 3, 27 -- bh=FYtPDGqDD30Dzu88HqWm+shucNHLy9hZt3NVOdJhC6Y=; 3, 27 -- b=X5AcftGmXmdCJBzp/o3OZ9ZOoRDE+QKF5eGUHj9zpkztc2vC/9FletPiPi4lR6N0a8G16szwIYtQZhSekmcckT/jBZaJkGaM0xM8KtyK2+OXAGtJgETHVpq+MhH1F7CRl198We4dFvKpuXVnx4wtIaYwq9p58M2AR0kHrUc5lD8= 3, 27 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 3, 27 -- d=gmail.com; s=gamma; 3, 27 -- h=message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 3, 27 -- b=U+sLkfSEw5VwotTATdOJGxwxBbZ4F4rNGetrN/vO8SVtQ9W2vd7yf1w0LXGBiQgH2Ap4Ml04FjWEJDWlF7KQFApbF/4aTLyQRQSuCpt7iXdA03QFHVaxlQ1IHGcHBPSOPa8AY/Hv3rbEsXmRuZI5NXv6UQ7vO9mP271emE0D57s= 3, 27 -- Received: by 10.140.180.42 with SMTP id c42mr3461545rvf.145.1201530449361; 3, 27 -- Mon, 28 Jan 2008 06:27:29 -0800 (PST) 3, 27 -- Received: by 10.141.13.19 with HTTP; Mon, 28 Jan 2008 06:27:29 -0800 (PST) 3, 27 -- Message-ID: {25e2b0d20801280627xe92e3b5y529ee11de5ab638b-at-mail.gmail.com} 3, 27 -- Date: Mon, 28 Jan 2008 09:27:29 -0500 3, 27 -- From: "Justin Kraft" {kraftpiano-at-gmail.com} 3, 27 -- To: microscopy-at-microscopy.com 3, 27 -- Subject: Chiller pump and lifting power. 3, 27 -- MIME-Version: 1.0 3, 27 -- Content-Type: text/plain; charset=ISO-8859-1 3, 27 -- Content-Transfer-Encoding: 7bit 3, 27 -- Content-Disposition: inline ==============================End of - Headers==============================
I'm sure the chiller manufacturer can answer this question for you. Our own experience in doing exactly the same thing was that we were able to run our (Haskris) chiller lines through the ceiling and move the chiller two rooms away----about 20 feet as the crow walks---with no problems whatsoever. However, when we needed that room for a new scope and moved the chiller again, we went a room too far with that extra 10-odd feet. The TEM began shutting down its lenses at frequent intervals and our JEOL engineer quickly figured out that the water flow was reduced to the point that we were running right at the edge of the water's ability to cool the lenses. A few degrees and the scope would shut them down. We were due to install a new TEM anyway, so he tweaked the temperature settings to allow a slight extra increase in temperature before it shut down, and we limped over the finish line until the new scope came.
Another caution--- when the lines run overhead be very careful to check for the development of leaks. Water spraying down on equipment can be fundamentally different that water puddling on the floor (we've had both happen and we've been lucky each time). Copper lines have the nasty tendency to occasionally develop pinhole leaks from being etched internally by distilled water.
So, yes, problems are possible, but it will be a function of chiller pumping capacity and distance from the scope. Again, check with the manufacturer.
Good luck!
Randy
Randy Tindall Senior EM Specialist Electron Microscopy Core Facility---We Do Small Well! W125 Veterinary Medicine University of Missouri Columbia, MO 65211 Tel: (573) 882-8304 Fax: (573) 884-2227 Email: tindallr-at-missouri.edu Web: http://www.emc.missouri.edu On-line calendar: http://biotech.rnet.missouri.edu/cgi-bin/Calcium310.pl?Op=Splash&Amount= Week&NavType=Both&Type=TimePlan
-----Original Message----- X-from: kraftpiano-at-gmail.com [mailto:kraftpiano-at-gmail.com] Sent: Monday, January 28, 2008 8:28 AM To: Tindall, Randy D.
I finally got sick of the noise and heat being thrown off by my water chiller system for the SEM, so I decided to move it to another room. Before I do this, though, the water lines have to be run up into the ceiling, over about 20 feet, then down to the microscope again. I've taken into consideration the connection end and plumbed in a valve system so I have a cut-off to run water through the chiller and piping without circulating it through the SEM, but I'm still wondering about the lifting power of the pump. Does the power required to lift the water lower the flow rate, or does the drop on the other end create enough of a siphon effect to cancel out any effects of the lift?
I seem to be able to reason this one out either way, depending on which outcome I'm looking for...
--Justin A. Kraft
==============================Original Headers============================== 3, 27 -- From kraftpiano-at-gmail.com Mon Jan 28 08:27:32 2008 3, 27 -- Received: from rv-out-0910.google.com (rv-out-0910.google.com [209.85.198.186]) 3, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0SERWxS027504 3, 27 -- for {microscopy-at-microscopy.com} ; Mon, 28 Jan 2008 08:27:32 -0600 3, 27 -- Received: by rv-out-0910.google.com with SMTP id k20so1635869rvb.30 3, 27 -- for {microscopy-at-microscopy.com} ; Mon, 28 Jan 2008 06:27:29 -0800 (PST) 3, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 3, 27 -- d=gmail.com; s=gamma; 3, 27 -- h=domainkey-signature:received:received:message-id:date:from:to:subject: mime-version:content-type:content-transfer-encoding:content-disposition; 3, 27 -- bh=FYtPDGqDD30Dzu88HqWm+shucNHLy9hZt3NVOdJhC6Y=; 3, 27 -- b=X5AcftGmXmdCJBzp/o3OZ9ZOoRDE+QKF5eGUHj9zpkztc2vC/9FletPiPi4lR6N0a8G16s zwIYtQZhSekmcckT/jBZaJkGaM0xM8KtyK2+OXAGtJgETHVpq+MhH1F7CRl198We4dFvKpuX Vnx4wtIaYwq9p58M2AR0kHrUc5lD8= 3, 27 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 3, 27 -- d=gmail.com; s=gamma; 3, 27 -- h=message-id:date:from:to:subject:mime-version:content-type:content-tran sfer-encoding:content-disposition; 3, 27 -- b=U+sLkfSEw5VwotTATdOJGxwxBbZ4F4rNGetrN/vO8SVtQ9W2vd7yf1w0LXGBiQgH2Ap4Ml 04FjWEJDWlF7KQFApbF/4aTLyQRQSuCpt7iXdA03QFHVaxlQ1IHGcHBPSOPa8AY/Hv3rbEsX mRuZI5NXv6UQ7vO9mP271emE0D57s= 3, 27 -- Received: by 10.140.180.42 with SMTP id c42mr3461545rvf.145.1201530449361; 3, 27 -- Mon, 28 Jan 2008 06:27:29 -0800 (PST) 3, 27 -- Received: by 10.141.13.19 with HTTP; Mon, 28 Jan 2008 06:27:29 -0800 (PST) 3, 27 -- Message-ID: {25e2b0d20801280627xe92e3b5y529ee11de5ab638b-at-mail.gmail.com} 3, 27 -- Date: Mon, 28 Jan 2008 09:27:29 -0500 3, 27 -- From: "Justin Kraft" {kraftpiano-at-gmail.com} 3, 27 -- To: microscopy-at-microscopy.com 3, 27 -- Subject: Chiller pump and lifting power. 3, 27 -- MIME-Version: 1.0 3, 27 -- Content-Type: text/plain; charset=ISO-8859-1 3, 27 -- Content-Transfer-Encoding: 7bit 3, 27 -- Content-Disposition: inline ==============================End of - Headers==============================
==============================Original Headers============================== 17, 26 -- From TindallR-at-missouri.edu Mon Jan 28 08:46:08 2008 17, 26 -- Received: from um-nsmtpout1.um.umsystem.edu (um-nsmtpout1.um.umsystem.edu [209.106.228.53]) 17, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0SEk7ep008180 17, 26 -- for {microscopy-at-microscopy.com} ; Mon, 28 Jan 2008 08:46:08 -0600 17, 26 -- Received: from UM-XMAIL08.um.umsystem.edu ([209.106.228.34]) by um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); 17, 26 -- Mon, 28 Jan 2008 08:46:07 -0600 17, 26 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 17, 26 -- Content-class: urn:content-classes:message 17, 26 -- MIME-Version: 1.0 17, 26 -- Content-Type: text/plain; 17, 26 -- charset="US-ASCII" 17, 26 -- Subject: RE: [Microscopy] Chiller pump and lifting power. 17, 26 -- Date: Mon, 28 Jan 2008 08:46:07 -0600 17, 26 -- Message-ID: {91108EF9255B394CBF8B7E3789814A4103CD7318-at-UM-XMAIL08.um.umsystem.edu} 17, 26 -- In-Reply-To: {200801281428.m0SESQQF029299-at-ns.microscopy.com} 17, 26 -- X-MS-Has-Attach: 17, 26 -- X-MS-TNEF-Correlator: 17, 26 -- Thread-Topic: [Microscopy] Chiller pump and lifting power. 17, 26 -- Thread-Index: Achhugvw/AQrtpSuR16k2kxC70lBTgAAEj+A 17, 26 -- References: {200801281428.m0SESQQF029299-at-ns.microscopy.com} 17, 26 -- From: "Tindall, Randy D." {TindallR-at-missouri.edu} 17, 26 -- To: {kraftpiano-at-gmail.com} 17, 26 -- Cc: {microscopy-at-microscopy.com} 17, 26 -- X-OriginalArrivalTime: 28 Jan 2008 14:46:07.0452 (UTC) FILETIME=[83E359C0:01C861BC] 17, 26 -- Content-Transfer-Encoding: 8bit 17, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m0SEk7ep008180 ==============================End of - Headers==============================
X-from a plumbing point of view, your latter comment is correct: if the inlet and outlet are at the same height as before, there is no extra consideration from running the hose up and down. If the piping system were open, the height of the loop would be a factor. If there is a difference in height between inlet and outlet, that must be taken into account.
However, the bigger concern in this case is the pressure drop due to the sheer length of tubing involved. Moving the chiller to another room and adding 15 to 20 feet just to loop over the wall adds a lot of extra flow restriction. Extra fittings may also be significant. Chemical engineers have tables of equivalent tube lengths for the many kinds of fittings. I have forgotten the details, but each fitting adds a substantial length (maybe up to a foot) to the effective length of the loop. It is good to keep them to a minimum.
The pressure drop can be handled by switching to a larger pump or by switching to a larger size hose. However, neither option is cheap. Consider it as you make your plans.
Warren Straszheim
-----Original Message----- X-from: kraftpiano-at-gmail.com [mailto:kraftpiano-at-gmail.com] Sent: Monday, January 28, 2008 8:28 AM To: wesaia-at-iastate.edu
I finally got sick of the noise and heat being thrown off by my water chiller system for the SEM, so I decided to move it to another room. Before I do this, though, the water lines have to be run up into the ceiling, over about 20 feet, then down to the microscope again. I've taken into consideration the connection end and plumbed in a valve system so I have a cut-off to run water through the chiller and piping without circulating it through the SEM, but I'm still wondering about the lifting power of the pump. Does the power required to lift the water lower the flow rate, or does the drop on the other end create enough of a siphon effect to cancel out any effects of the lift?
I seem to be able to reason this one out either way, depending on which outcome I'm looking for...
--Justin A. Kraft
==============================Original Headers============================== 10, 37 -- From wesaia-at-iastate.edu Mon Jan 28 09:48:00 2008 10, 37 -- Received: from mailhub-3.iastate.edu (mailhub-3.iastate.edu [129.186.140.13]) 10, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0SFm0au032609 10, 37 -- for {Microscopy-at-msa.microscopy.com} ; Mon, 28 Jan 2008 09:48:00 -0600 10, 37 -- Received: from devirus-10.iastate.edu (devirus-10.iastate.edu [129.186.1.47]) 10, 37 -- by mailhub-3.iastate.edu (8.12.11.20060614/8.12.10) with SMTP id m0SFlwXu021996; 10, 37 -- Mon, 28 Jan 2008 09:47:58 -0600 10, 37 -- Received: from (despam-10.iastate.edu [129.186.140.80]) by devirus-10.iastate.edu with smtp 10, 37 -- id 4d3b_fa867f32_cdb7_11dc_91cd_00137253420a; 10, 37 -- Mon, 28 Jan 2008 09:44:57 -0600 10, 37 -- Received: from owa.eng.iastate.edu (owa.eng.iastate.edu [129.186.23.85]) 10, 37 -- by despam-10.iastate.edu (8.12.11.20060614/8.12.10) with ESMTP id m0SFlso2027001; 10, 37 -- Mon, 28 Jan 2008 09:47:56 -0600 10, 37 -- Received: from maire.eng.iastate.edu ([10.10.196.69]) by owa.eng.iastate.edu with Microsoft SMTPSVC(6.0.3790.3959); 10, 37 -- Mon, 28 Jan 2008 09:46:35 -0600 10, 37 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 10, 37 -- Content-class: urn:content-classes:message 10, 37 -- MIME-Version: 1.0 10, 37 -- Content-Type: text/plain; 10, 37 -- charset="us-ascii" 10, 37 -- Subject: RE: [Microscopy] Chiller pump and lifting power. 10, 37 -- Date: Mon, 28 Jan 2008 09:46:36 -0600 10, 37 -- Message-ID: {16A330AC32056A40B32842EC4BB8D7270277488F-at-maire.eng.iastate.edu} 10, 37 -- In-Reply-To: {200801281428.m0SESDkE028709-at-ns.microscopy.com} 10, 37 -- X-MS-Has-Attach: 10, 37 -- X-MS-TNEF-Correlator: 10, 37 -- Thread-Topic: [Microscopy] Chiller pump and lifting power. 10, 37 -- Thread-Index: AchhugSu2bTucrHASEWOYXRAREX59wABgtog 10, 37 -- References: {200801281428.m0SESDkE028709-at-ns.microscopy.com} 10, 37 -- From: "Straszheim, Warren E [M S E]" {wesaia-at-iastate.edu} 10, 37 -- To: "MSA" {Microscopy-at-msa.microscopy.com} 10, 37 -- Cc: {kraftpiano-at-gmail.com} 10, 37 -- X-OriginalArrivalTime: 28 Jan 2008 15:46:35.0057 (UTC) FILETIME=[F61BF210:01C861C4] 10, 37 -- X-PMX-Version: 5.3.2.304607, Antispam-Engine: 2.5.1.298604, Antispam-Data: 2008.1.28.73554 10, 37 -- X-ISUMailhub-test: Gauge=IIIIIII, Probability=7%, Report='__CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __IMS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0' 10, 37 -- Content-Transfer-Encoding: 8bit 10, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m0SFm0au032609 ==============================End of - Headers==============================
Like Malcolm, I know of no ergonomic guidelines that are specific to microscopy. However, folks in our lab have much experience in the areas of optical, AFM, SEM, TEM and microtomy. We receive generalized training in ergonomics at my work. However, I have significant personal experience with ergo problems and know first-hand how painful and long-lasting the problems can become.
Ergonomically healthy posture when using a stand-alone optical microscope, TEM or microtome, i.e. without a computer interface, is relatively straightforward to achieve. Good, relaxed posture is important. Ensure that a chair equipped with arm rests is available that allows each analyst to look through the oculars without hunching over or straining upward. Minimizing eye strain is essential for long periods of time at an optical microscope. Ensure that each analyst knows how to adjust the binoculars to ones interpupillary distance and focus for each eye. Try to minimize bright lighting above or around the microscope. Glare and too-bright ambient lighting can pose problems during extending work on a microscope or computer.
Unfortunately, interfacing a computer, monitor, keyboard and mouse turns a relatively ergonomically simple instrument into a nightmare for posture. Our internal ergo consultants have provided some help although they contend that computerization of microscopes generates hard-to-resolve problems. The problem that occurs when one integrates a computer with any type of microscope is that the microscope and computer, ergonomically benign tools when used correctly, each become difficult to use. It seems that one may choose to be comfortable at one or the other but not both. Trade-offs tend to be the rule. I suggest that one achieve the best ergo-friendly setup for the tool that is used most frequently. If one tends to spend most of the time at the microscope and only intermittently work at the computer, then design the ergonomics of the work area around the scope; the converse should be applied if the primary tool used during analyses is the computer.
Several key points should be made. Please note that repeated reaching or twisting means even several times during multiple short sessions at the scope. Ergo problems tend to be cumulative during a day or even days: Never repeatedly twist the body to reach a tool. Rather, roll the chair to the tool and assume good posture. It takes a little longer but will be worthwhile in the end. Avoid repeated reaching, i.e. extending ones arm, to perform a task. This can quickly lead to severe shoulder and neck pain. Take a short break each half hour on the scope. Spending a couple of minutes to stretch the hands, shoulders, back and neck can work wonders. Finally, be aware of minor aches and pains that didn't seem to be there before. A hand, thumb, arm, shoulder, back or neck that is even slightly stiff or sore should be stretched and rested before continuing extensive work on the scope. Varying the type of work done during the day can be a big help in minimizing or eliminating ergo problems. A set of cold eyes is often useful to recognize problems of which the individual may not aware.
Several guidelines that we have found helpful in the use of computers follow: The computer monitor should be adjustable for each, not most, microscopist. Set the monitor height such that the top of the monitor field is an inch or so higher than the persons eyes when looking straight ahead. Avoid looking up or down at a monitor. I have found that looking upward at a monitor may cause very significant issues with the neck and shoulders, sometimes in a very short period of time. Like monitors, keyboards and mouse(mouses, mice ?) should be adjustable for each (not most) microscopist. Avoid reaching or extending the arms to reach the keyboard or mouse. Adjustable keyboard articulating trays are very useful. The keyboard and mouse should be set to neutral posture such that the lower arms are horizontally orientally and the tops of the hands do not bend up or down. Consider using fitting the computer with a trackball in addition to or instead of a mouse. When selecting a trackball, look for one with the largest ball available. The ball should be one that is moved with the fingers and/or hands, not the thumb. I use the Kensington Expert Mouse (I know that other good trackballs exist). The ball is about the size of a billiard ball.
Keep in mind that solving and correcting ergo problems in microscopy labs often requires patience and perseverance.
Best regards,
Disclaimer: The comments and opinions given above are those of the author alone and do not represent any position of ExxonMobil Chemical Company.
Gary M. Brown Microscopy Specialist ExxonMobil Chemical Company Baytown Technology & Engineering - West 5200 Bayway Drive Baytown, Texas 77520-2101 phone: 281 834 2387 fax: 281 834 2395 e-mail: Gary.M.Brown-at-ExxonMobil.com
"Our business in life is not to succeed, but to continue to fail in good spirits." Robert Louis Stevenson
----- Forwarded by Gary M Brown/Baytown/ExxonMobil on 01/28/08 10:11 AM -----
malcolm.haswel l-at-sunderland.a c.uk To gary.m.brown-at-exxonmobil.com cc 01/28/08 05:10 AM Subject [Microscopy] Re: AskAMicroscopist: Microscope Ergonomics Please respond to malcolm.haswel l-at-sunderland.a c.uk
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
David
I am not sure of any specific health and safety regulations issued by any any safety organizations.
But I would suggest that appropriate or similar regulations such as would be useful: 1. The provision, use and maintenance of workplace equipment (PUWER 1998 UK safety regulations) 2. Display Screen Equipment (computers and similar instruments) regulations (DSE 2002 UK safety regulations) 3. and specific safety handling regulations for specimens such as chemical, biological and microbiological where appropriate in the lab.
I did a quick Google search (see below) and found a few industry and university websites. They all seem to consider ergonomics, optimal operation, good maintenance, environment, work activity patterns and nature of the specimen and any chemicals as most important. Most users seem to prefer binocular eyepieces with a good range of easy to use adjustments (eg dioptre correction and interocular distance.
I hope this helps and sorry about all the UK safety references but I'm sure there will be similar US regulations.
Good luck
Malcolm
Malcolm Haswell e.m. unit Chemispec School of Health, Natural and Social Sciences Fleming Building University of Sunderland Tyne & Wear SR1 3SD UK e-mail: malcolm.haswell-at-sunderland.ac.uk
----- Original Message ----- X-from: davefissell-at-yahoo.com
Hi,
The short answer is that it takes only 5 PSI of pressure for every 10 feet (one story tall) of vertical head or drop. So you only need 5 PSI extra to push "up hill". I am sure you have that much pressure. Suppose the chiller output is 50 PSI. The pressure at the top of the pipe would be the output of the chiller under normal SEM conditions minus 5 PSI. You will gain that 5 PSI back on the drop side of the inverted U tube. So the pressure at the SEM input should still be 50 PSI. If it's lower than 50, you could still have an air lock (see below). If you keep your bypass open, then the pressure will drop. Keep it closed and the SEM on-line during pressure measurements. The pressure losses from the delivery lines should be minor unless you use really small diameter pipes or have a lot of bends. Stick with 1/2" ID plastic, not copper. Use pipe rated for at least 100 PSI.
Your problem might be in eliminating the initial inverted U loop's air lock at the top of the loop. You might consider installing a tee and globe valve at the top of the loop to bleed off *most* of the tapped air.
Paul
At 08:28 AM 1/28/08 -0600, you wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 5, 28 -- From beaurega-at-westol.com Mon Jan 28 12:52:53 2008 5, 28 -- Received: from smtp-gateway-7.winbeam.com (smtp-gateway-7.winbeam.com [64.84.97.73]) 5, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0SIqpUH006309 5, 28 -- for {microscopy-at-microscopy.com} ; Mon, 28 Jan 2008 12:52:52 -0600 5, 28 -- X-Winbeam-MailScanner-Watermark: 1202151145.04289-at-xq9W19+GjPQfEER6/X3TDg 5, 28 -- Received: from mail.winbeam.com (mail.winbeam.com [64.84.96.10]) 5, 28 -- by smtp-gateway-7.winbeam.com (8.13.1/8.12.8) with SMTP id m0SIqKlJ020496 5, 28 -- for {microscopy-at-microscopy.com} ; Mon, 28 Jan 2008 13:52:23 -0500 5, 28 -- Received: (qmail 15267 invoked by uid 89); 28 Jan 2008 18:52:21 -0000 5, 28 -- Received: from pitts-69-72-12-130.dynamic-dialup.coretel.net (HELO millenium) (69.72.12.130) 5, 28 -- by mail.winbeam.com with SMTP; 28 Jan 2008 18:52:21 -0000 5, 28 -- Message-Id: {3.0.6.32.20080128135230.007d4720-at-pop3.norton.antivirus} 5, 28 -- X-Sender: beaurega/mail.westol.com-at-pop3.norton.antivirus 5, 28 -- X-Mailer: QUALCOMM Windows Eudora Light Version 3.0.6 (32) 5, 28 -- Date: Mon, 28 Jan 2008 13:52:30 -0500 5, 28 -- To: microscopy-at-microscopy.com 5, 28 -- From: Beaurega {beaurega-at-westol.com} 5, 28 -- Subject: Re: [Microscopy] Chiller pump and lifting power. 5, 28 -- In-Reply-To: {200801281428.m0SES07q028210-at-ns.microscopy.com} 5, 28 -- Mime-Version: 1.0 5, 28 -- Content-Type: text/plain; charset="us-ascii" 5, 28 -- X-Winbeam-MailScanner-Information: Winbeam - Please contact Technical Support for more information 5, 28 -- X-MailScanner-ID: m0SIqKlJ020496 5, 28 -- X-Winbeam-MailScanner: Found to be clean Winbeam (courtesy of MailScanner) 5, 28 -- X-Winbeam-MailScanner-SpamCheck: not spam (whitelisted), 5, 28 -- SpamAssassin (not cached, score=-0.5, required 4, autolearn=not spam, 5, 28 -- BAYES_00 -0.50) 5, 28 -- X-Winbeam-MailScanner-From: beaurega-at-westol.com ==============================End of - Headers==============================
Hi, we have recently started to work on corpus callosum formation in the developing mamalian forebrain. First we performed a pilot study to see the callosol axon projections on E 14 and E 17 mouse foetus brain regarding the reference studies. We could see the callosal axons on the coronal sections as shown in the references . But we decided to run the study with rat foetuses instead of mice because of the lack of the facilities. However we could not find any spesific timing information in the literature about CC formation in rats . We wonder when CC spesificly begins and ends to form during the rat gestation and also where we suppose to see the growing callosal axons on E 14, 15 and 17 on the coronal histological sections in rats.
I would greatly appreciate if you could inform me about these issues .
Thank you in advance for any information you can suggest me.
Yours Sincerely
Dr. Necat Yilmaz Mersin Universitesi Tip Fakultesi Histoloji ve Embriyoloji Anabilim Dali
==============================Original Headers============================== 12, 31 -- From nyilmaz-at-mersin.edu.tr Mon Jan 28 13:12:20 2008 12, 31 -- Received: from mail.mersin.edu.tr (mail.mersin.edu.tr [193.255.128.3]) 12, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0SJCJ7D019510 12, 31 -- for {Microscopy-at-microscopy.com} ; Mon, 28 Jan 2008 13:12:20 -0600 12, 31 -- Received: from localhost (localhost.mersin.edu.tr [127.0.0.1]) 12, 31 -- by mail.mersin.edu.tr (Postfix) with ESMTP id 7702B3647A3; 12, 31 -- Mon, 28 Jan 2008 21:12:37 +0200 (EET) 12, 31 -- X-Virus-Scanned: amavisd-new at mersin.edu.tr 12, 31 -- Received: from mail.mersin.edu.tr ([127.0.0.1]) 12, 31 -- by localhost (yenimail.mersin.edu.tr [127.0.0.1]) (amavisd-new, port 10024) 12, 31 -- with ESMTP id oL8gEgGfzLXP; Mon, 28 Jan 2008 21:12:08 +0200 (EET) 12, 31 -- Received: from NEJAT1 (unknown [88.227.7.183]) 12, 31 -- by mail.mersin.edu.tr (Postfix) with SMTP id 2FAFD36479E; 12, 31 -- Mon, 28 Jan 2008 21:12:08 +0200 (EET) 12, 31 -- Message-ID: {004301c861e1$a2b42530$0401a8c0-at-NEJAT1} 12, 31 -- From: "Nejat" {nyilmaz-at-mersin.edu.tr} 12, 31 -- To: {histonet-at-lists.utsouthwestern.edu} , 12, 31 -- "EM-Mail Group" {Microscopy-at-microscopy.com} 12, 31 -- Subject: Rat corpus callosum formation 12, 31 -- Date: Mon, 28 Jan 2008 21:10:57 +0200 12, 31 -- MIME-Version: 1.0 12, 31 -- Content-Type: text/plain; 12, 31 -- format=flowed; 12, 31 -- charset="windows-1254"; 12, 31 -- reply-type=original 12, 31 -- Content-Transfer-Encoding: 7bit 12, 31 -- X-Priority: 3 12, 31 -- X-MSMail-Priority: Normal 12, 31 -- X-Mailer: Microsoft Outlook Express 6.00.2900.3138 12, 31 -- Disposition-Notification-To: "Nejat" {nyilmaz-at-mersin.edu.tr} 12, 31 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 ==============================End of - Headers==============================
Anyone know of a plugin for the free image analysis program ImageTool that will allow it perform automatic particle size and aspect ratio(height to diameter) calculations? I understand this program can use Photoshop plugins also. Thanks in advance.
Gary M. Easton
Scanners Corporation Independent SEM Service 30 years experience Cambridge SEM's our specialty 410-857-7633
==============================Original Headers============================== 7, 21 -- From garyeaston-at-scannerscorp.com Mon Jan 28 15:34:33 2008 7, 21 -- Received: from omr14.networksolutionsemail.com (omr14.networksolutionsemail.com [205.178.146.64]) 7, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0SLYXkX006297 7, 21 -- for {microscopy-at-microscopy.com} ; Mon, 28 Jan 2008 15:34:33 -0600 7, 21 -- Received: from mail.networksolutionsemail.com (ns-omr14.mgt.hosting.dc2.netsol.com [10.49.6.77]) 7, 21 -- by omr14.networksolutionsemail.com (8.13.6/8.13.6) with SMTP id m0SLYXEX000470 7, 21 -- for {microscopy-at-microscopy.com} ; Mon, 28 Jan 2008 16:34:33 -0500 7, 21 -- Received: (qmail 31552 invoked by uid 78); 28 Jan 2008 21:34:32 -0000 7, 21 -- Received: from unknown (HELO ?127.0.0.1?) (70.22.75.237) 7, 21 -- by ns-omr14.lb.hosting.dc2.netsol.com with SMTP; 28 Jan 2008 21:34:32 -0000 7, 21 -- Message-ID: {479E4A67.2020901-at-scannerscorp.com} 7, 21 -- Date: Mon, 28 Jan 2008 16:34:31 -0500 7, 21 -- From: "Gary M. Easton" {garyeaston-at-scannerscorp.com} 7, 21 -- User-Agent: Thunderbird 2.0.0.9 (Windows/20071031) 7, 21 -- MIME-Version: 1.0 7, 21 -- To: Microscopy Society of America {microscopy-at-microscopy.com} 7, 21 -- Subject: Question about ImageTool 7, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 21 -- Content-Transfer-Encoding: 7bit 7, 21 -- X-Antivirus: avast! (VPS 080128-0, 01/28/2008), Outbound message 7, 21 -- X-Antivirus-Status: Clean ==============================End of - Headers==============================
On Jan 28, 2008, at 6:27 AM, kraftpiano-at-gmail.com wrote:
} I finally got sick of the noise and heat being thrown off by my water } chiller system for the SEM, so I decided to move it to another room. } Before I do this, though, the water lines have to be run up into the } ceiling, over about 20 feet, then down to the microscope again. I've } taken into consideration the connection end and plumbed in a valve } system so I have a cut-off to run water through the chiller and piping } without circulating it through the SEM, but I'm still wondering about } the lifting power of the pump. Does the power required to lift the } water lower the flow rate, or does the drop on the other end create } enough of a siphon effect to cancel out any effects of the lift? } } I seem to be able to reason this one out either way, depending on } which outcome I'm looking for...
Dear Justin, The chillers for the high-voltage scope in Albany were located in a sub-sub basement about 10 meters below the lowest point of the scope, and the water distribution manifold for the lenses was located another 5 meters above that. There was no difficulty supplying water to any part of the scope. The pump on the chiller pushed water up to the manifold, so the height is not a problem, and, as long as there is no way air can get into the lines, each liter of water pushed into the line forces one liter to be pushed into the tank, so the water should circulate the same way regardless of the height it is raised to. The flow rate can be affected by the length of the lines due to viscosity; i.e., the pump must provide enough power to overcome the resistance due to the friction of the water flowing through the lines, which is higher for longer and thinner lines. Yours, Bill Tivol, PhD EM Scientist Electron Cryo-Microscopy Facility Broad Center, Mail Code 114-96 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 6, 22 -- From tivol-at-caltech.edu Mon Jan 28 15:35:53 2008 6, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0SLZrHi007990 6, 22 -- for {microscopy-at-msa.microscopy.com} ; Mon, 28 Jan 2008 15:35:53 -0600 6, 22 -- Received: from fire-dog.its.caltech.edu (fire-dog [192.168.1.4]) 6, 22 -- by water-ox-postvirus (Postfix) with ESMTP id 85A541B91D 6, 22 -- for {microscopy-at-msa.microscopy.com} ; Mon, 28 Jan 2008 13:35:51 -0800 (PST) 6, 22 -- Received: from [192.168.159.158] (jpix-01.caltech.edu [131.215.2.133]) 6, 22 -- by fire-ox.its.caltech.edu (Postfix) with ESMTP id 12DF013E70 6, 22 -- for {microscopy-at-msa.microscopy.com} ; Mon, 28 Jan 2008 13:35:48 -0800 (PST) 6, 22 -- Mime-Version: 1.0 (Apple Message framework v753) 6, 22 -- In-Reply-To: {200801281427.m0SERjMg027658-at-ns.microscopy.com} 6, 22 -- References: {200801281427.m0SERjMg027658-at-ns.microscopy.com} 6, 22 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 6, 22 -- Message-Id: {A72D0999-8714-49D5-8162-0D71CB57ABB7-at-caltech.edu} 6, 22 -- Content-Transfer-Encoding: 7bit 6, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 6, 22 -- Subject: Re: [Microscopy] Chiller pump and lifting power. 6, 22 -- Date: Mon, 28 Jan 2008 13:35:54 -0800 6, 22 -- To: microscopy-at-msa.microscopy.com 6, 22 -- X-Mailer: Apple Mail (2.753) 6, 22 -- X-Spam-Scanned: at Caltech-ITS on fire-dog by amavisd-2.4.5 ==============================End of - Headers==============================
We have been having major problems with our in-line tap water filters becoming clogged (sometimes on a bi-weekly basis). These string-filters remove sediment from the tap water that is used to remove heat from our closed-loop water chillers. Our Physical Plant has not been able to locate the source of the sludge and we end up paying them several hundred dollars to come and change two sets of filters.
Does anyone know of a large capacity (self-cleaning) filtration system that could be used on the water coming into our small, microscopy building? Since our major use of water is probably the water chillers, it may be more advantageous to go this route rather than relying on the small, under-sink string filters.
Thank you,
John Bozzola -- +++++++++++++++++++++++++++++++++++++++++++++++++++++++
John J. Bozzola, Ph.D., Director Integrated Microscopy & Graphics Expertise (IMAGE) Southern Illinois University 750 Communications Drive - MC 4402 Carbondale, IL 62901 Telephone: 618-453-3730
This Question was submitted to Ask-A-Microscopist by (august04-at-verizon.net) from http://www.msa.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Monday, January 28, 2008 at 18:36:19 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both august04-at-verizon.net as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Email: august04-at-verizon.net Name: Charles Pique
Organization: None
Education: Graduate College
Location: Charleston, West Virginia, USA
Title: Brownian motion
Question: What magnification and conditions are needed to see the brownian motion?
As I recall, Brownian motion is in part due to the density and temperature of particles. The differentiation of particles is important from large objects (Gouy, 1889). The smaller the particles, the more impact external forces have on them. This includes thermal effects.
I think that the basic theory is formulated on particles in a fluid. If one takes this literally, one could collect rotifers and examine them under a microscope at perhaps 100X or less and study their movement. Then, increase the temperature of their medium and the movement will increase. The point is that there is no distinct and definitive direction for movement in either of the thermal conditions. Movement is random. There is no distinct vector for movement regardless of temperature.
In particular, the length of the movement is infinite over any length of interval. Being rather impossible, Brownian motion is purely an abstract concept. This is based on the inability of a particle to move an infinite distance in a finite length of time. Einstein had some publications about Brownian motion. You might do some research on/for these.
As an aside, some have identified the movement of employees in a company as Brownian motion...moving here and there to show activity but with no actual production output. But that is a stretch (?).
Let us know what you find out.
gary g
At 04:47 PM 1/28/2008, you wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 9, 20 -- From gary-at-gaugler.com Mon Jan 28 19:30:09 2008 9, 20 -- Received: from qsmtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 9, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m0T1U9ww030590 9, 20 -- for {microscopy-at-microscopy.com} ; Mon, 28 Jan 2008 19:30:09 -0600 9, 20 -- Message-Id: {200801290130.m0T1U9ww030590-at-ns.microscopy.com} 9, 20 -- Received: (qmail 2377 invoked from network); 28 Jan 2008 17:30:08 -0800 9, 20 -- Received: by simscan 1.1.0 ppid: 2374, pid: 2375, t: 0.1109s 9, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 9, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 9, 20 -- by qsmtp1 with SMTP; 28 Jan 2008 17:30:08 -0800 9, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 9, 20 -- Date: Mon, 28 Jan 2008 17:30:02 -0800 9, 20 -- To: august04-at-verizon.net 9, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 9, 20 -- Subject: Re: [Microscopy] AskAMicroscopist: Brownian motion 9, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 9, 20 -- In-Reply-To: {200801290047.m0T0lr1f019415-at-ns.microscopy.com} 9, 20 -- References: {200801290047.m0T0lr1f019415-at-ns.microscopy.com} 9, 20 -- Mime-Version: 1.0 9, 20 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-20F23A01 ==============================End of - Headers==============================
My swimming pool has some very large filters and I can clean them myself in a half hour. It would require shutting off the microscope, though. Better to locate the source of sludge.
John Mardinly,
-----Original Message----- X-from: bozzola-at-siu.edu [mailto:bozzola-at-siu.edu] Sent: Monday, January 28, 2008 2:06 PM To: Mardinly, John
We have been having major problems with our in-line tap water filters becoming clogged (sometimes on a bi-weekly basis). These string-filters remove sediment from the tap water that is used to remove heat from our closed-loop water chillers. Our Physical Plant has not been able to locate the source of the sludge and we end up paying them several hundred dollars to come and change two sets of filters.
Does anyone know of a large capacity (self-cleaning) filtration system that could be used on the water coming into our small, microscopy building? Since our major use of water is probably the water chillers, it may be more advantageous to go this route rather than relying on the small, under-sink string filters.
Thank you,
John Bozzola -- +++++++++++++++++++++++++++++++++++++++++++++++++++++++
John J. Bozzola, Ph.D., Director Integrated Microscopy & Graphics Expertise (IMAGE) Southern Illinois University 750 Communications Drive - MC 4402 Carbondale, IL 62901 Telephone: 618-453-3730
-----Original Message----- X-from: kraftpiano-at-gmail.com [mailto:kraftpiano-at-gmail.com] Sent: Monday, January 28, 2008 6:28 AM To: Mardinly, John
I finally got sick of the noise and heat being thrown off by my water chiller system for the SEM, so I decided to move it to another room. Before I do this, though, the water lines have to be run up into the ceiling, over about 20 feet, then down to the microscope again. I've taken into consideration the connection end and plumbed in a valve system so I have a cut-off to run water through the chiller and piping without circulating it through the SEM, but I'm still wondering about the lifting power of the pump. Does the power required to lift the water lower the flow rate, or does the drop on the other end create enough of a siphon effect to cancel out any effects of the lift?
I seem to be able to reason this one out either way, depending on which outcome I'm looking for...
--Justin A. Kraft
==============================Original Headers============================== 3, 27 -- From kraftpiano-at-gmail.com Mon Jan 28 08:27:32 2008 3, 27 -- Received: from rv-out-0910.google.com (rv-out-0910.google.com [209.85.198.186]) 3, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0SERWxS027504 3, 27 -- for {microscopy-at-microscopy.com} ; Mon, 28 Jan 2008 08:27:32 -0600 3, 27 -- Received: by rv-out-0910.google.com with SMTP id k20so1635869rvb.30 3, 27 -- for {microscopy-at-microscopy.com} ; Mon, 28 Jan 2008 06:27:29 -0800 (PST) 3, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 3, 27 -- d=gmail.com; s=gamma; 3, 27 -- h=domainkey-signature:received:received:message-id:date:from:to:subject: mime-version:content-type:content-transfer-encoding:content-disposition; 3, 27 -- bh=FYtPDGqDD30Dzu88HqWm+shucNHLy9hZt3NVOdJhC6Y=; 3, 27 -- b=X5AcftGmXmdCJBzp/o3OZ9ZOoRDE+QKF5eGUHj9zpkztc2vC/9FletPiPi4lR6N0a8G16s zwIYtQZhSekmcckT/jBZaJkGaM0xM8KtyK2+OXAGtJgETHVpq+MhH1F7CRl198We4dFvKpuX Vnx4wtIaYwq9p58M2AR0kHrUc5lD8= 3, 27 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 3, 27 -- d=gmail.com; s=gamma; 3, 27 -- h=message-id:date:from:to:subject:mime-version:content-type:content-tran sfer-encoding:content-disposition; 3, 27 -- b=U+sLkfSEw5VwotTATdOJGxwxBbZ4F4rNGetrN/vO8SVtQ9W2vd7yf1w0LXGBiQgH2Ap4Ml 04FjWEJDWlF7KQFApbF/4aTLyQRQSuCpt7iXdA03QFHVaxlQ1IHGcHBPSOPa8AY/Hv3rbEsX mRuZI5NXv6UQ7vO9mP271emE0D57s= 3, 27 -- Received: by 10.140.180.42 with SMTP id c42mr3461545rvf.145.1201530449361; 3, 27 -- Mon, 28 Jan 2008 06:27:29 -0800 (PST) 3, 27 -- Received: by 10.141.13.19 with HTTP; Mon, 28 Jan 2008 06:27:29 -0800 (PST) 3, 27 -- Message-ID: {25e2b0d20801280627xe92e3b5y529ee11de5ab638b-at-mail.gmail.com} 3, 27 -- Date: Mon, 28 Jan 2008 09:27:29 -0500 3, 27 -- From: "Justin Kraft" {kraftpiano-at-gmail.com} 3, 27 -- To: microscopy-at-microscopy.com 3, 27 -- Subject: Chiller pump and lifting power. 3, 27 -- MIME-Version: 1.0 3, 27 -- Content-Type: text/plain; charset=ISO-8859-1 3, 27 -- Content-Transfer-Encoding: 7bit 3, 27 -- Content-Disposition: inline ==============================End of - Headers==============================
==============================Original Headers============================== 12, 33 -- From john.mardinly-at-intel.com Mon Jan 28 19:55:18 2008 12, 33 -- Received: from mga09.intel.com (mga09.intel.com [134.134.136.24]) 12, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0T1tIww016745 12, 33 -- for {Microscopy-at-msa.microscopy.com} ; Mon, 28 Jan 2008 19:55:18 -0600 12, 33 -- Received: from fmsmga002.fm.intel.com ([10.253.24.26]) 12, 33 -- by orsmga102.jf.intel.com with ESMTP; 28 Jan 2008 17:55:05 -0800 12, 33 -- X-ExtLoop1: 1 12, 33 -- X-IronPort-AV: E=Sophos;i="4.25,265,1199692800"; 12, 33 -- d="scan'208";a="293828247" 12, 33 -- Received: from orsmsx335.jf.intel.com ([10.22.226.40]) 12, 33 -- by fmsmga002.fm.intel.com with ESMTP; 28 Jan 2008 17:53:06 -0800 12, 33 -- Received: from orsmsx423.amr.corp.intel.com ([10.22.226.104]) by orsmsx335.jf.intel.com with Microsoft SMTPSVC(6.0.3790.1830); 12, 33 -- Mon, 28 Jan 2008 17:54:04 -0800 12, 33 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 12, 33 -- Content-class: urn:content-classes:message 12, 33 -- MIME-Version: 1.0 12, 33 -- Content-Type: text/plain; 12, 33 -- charset="us-ascii" 12, 33 -- Subject: RE: [Microscopy] Chiller pump and lifting power. 12, 33 -- Date: Mon, 28 Jan 2008 17:54:03 -0800 12, 33 -- Message-ID: {36C438BD1408B1499A19BA5730AAFC63AE0BF1-at-orsmsx423.amr.corp.intel.com} 12, 33 -- In-Reply-To: {200801281427.m0SERiTd027631-at-ns.microscopy.com} 12, 33 -- X-MS-Has-Attach: 12, 33 -- X-MS-TNEF-Correlator: 12, 33 -- Thread-Topic: [Microscopy] Chiller pump and lifting power. 12, 33 -- Thread-Index: AchhufN2O1HAfgawS0G1eCfRnwMCPQAX7MAA 12, 33 -- References: {200801281427.m0SERiTd027631-at-ns.microscopy.com} 12, 33 -- From: "Mardinly, John" {john.mardinly-at-intel.com} 12, 33 -- To: {kraftpiano-at-gmail.com} 12, 33 -- Cc: {Microscopy-at-msa.microscopy.com} 12, 33 -- X-OriginalArrivalTime: 29 Jan 2008 01:54:04.0532 (UTC) FILETIME=[D3B07340:01C86219] 12, 33 -- Content-Transfer-Encoding: 8bit 12, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m0T1tIww016745 ==============================End of - Headers==============================
Our water line currently has one filter and no bypass; on days when I've had enough coffee to be especially lucid I can change a filter without the chillers noticing. However it is directly over a 208 3-phase outlet so while rushing to do the job fast, water drips and I end up standing in a pool of water while watching it drip into the outlet - not great.
I sketched out a plan (as yet unimplemented) for a system with 2 alternate branches so that the flow can be switched to an unused filter unit while the loop with the used filter is isolated and the filter replaced. I have planned a drain/flush spigot on the inlet side so I can flush the line upstream of the filters from time to time, or before a filter change. The standard filters are really cheap at McMaster-Carr (a couple of dollars, I think; I get a case at a time, we also have 'enriched' water). I can send the Prod# - we use a spun plastic (5um I think) instead of the string filters.
It is going to be hard to beat the economy of standard mass-produced filter units for a low volume application supporting some chillers, etc. If you have the usual 'before and after' pressure gages you can see the pressure drop developing across the active filter and change filters before trouble comes knocking.
Dale Callaham UMASS, Amherst
bozzola-at-siu.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } We have been having major problems with our in-line tap water filters } becoming clogged (sometimes on a bi-weekly basis). These } string-filters remove sediment from the tap water that is used to } remove heat from our closed-loop water chillers. Our Physical Plant } has not been able to locate the source of the sludge and we end up } paying them several hundred dollars to come and change two sets of } filters. } } Does anyone know of a large capacity (self-cleaning) filtration } system that could be used on the water coming into our small, } microscopy building? Since our major use of water is probably the } water chillers, it may be more advantageous to go this route rather } than relying on the small, under-sink string filters. } } Thank you, } } John Bozzola
==============================Original Headers============================== 6, 20 -- From dac-at-research.umass.edu Mon Jan 28 20:03:23 2008 6, 20 -- Received: from race1.oit.umass.edu (race1.oit.umass.edu [128.119.101.37]) 6, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0T23N8c003930 6, 20 -- for {microscopy-at-microscopy.com} ; Mon, 28 Jan 2008 20:03:23 -0600 6, 20 -- Received: from [192.168.1.23] (68-189-254-59.dhcp.oxfr.ma.charter.com [68.189.254.59]) 6, 20 -- (authenticated bits=0) 6, 20 -- by race1.oit.umass.edu (8.14.1/8.14.1) with ESMTP id m0T23MXu022800 6, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT); 6, 20 -- Mon, 28 Jan 2008 21:03:22 -0500 6, 20 -- Message-ID: {479E896E.8020000-at-research.umass.edu} 6, 20 -- Date: Mon, 28 Jan 2008 21:03:26 -0500 6, 20 -- From: Dale Callaham {dac-at-research.umass.edu} 6, 20 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.0; en-US; rv:1.8.1.11) Gecko/20071128 SeaMonkey/1.1.7 6, 20 -- MIME-Version: 1.0 6, 20 -- To: bozzola-at-siu.edu, Microscopy List {microscopy-at-microscopy.com} 6, 20 -- Subject: Re: [Microscopy] large capacity tap water filters 6, 20 -- References: {200801282211.m0SMB9Kk008641-at-ns.microscopy.com} 6, 20 -- In-Reply-To: {200801282211.m0SMB9Kk008641-at-ns.microscopy.com} 6, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 20 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
In my experience a rock thin section consisting mostly of quartz will offer opportunities to see this at magnifications of about 400x and above - occasionally even lower. Quartz that is formed hydrothermally (by crystalization from silica-bearing water deep in the earth) typically contains fluid inclusions. These are visible as voids inside the quartz. Often they are not completely filled by liquid and the remaining space is occupied by a bubble of gas. Brownian motion will be apparent from the agitation of the bubble--- it will appear to be constantly jostled about by some unseen forces. If the heat from the microscope illuminator is not completely eliminated by a filter, the jostling will become more violent as the thin section warms up and decrease when the light is removed for a time.
You will need to view the thin section in transmitted light but it is not necessary to have an actual petrographic microscope to see the effect, a biological scope will do. Some other stone sections will exhibit two-phase (gas-liquid) inclusions. Calcite, for example may contain gas-liquid inclusions or liquid-liquid inclusions. The latter consist of immiscible liquids such as brine and petroleum. However, the low mass of a gas bubble in a fluid inclusion makes it a better candidate to observe the motion and quartz more often provides a clear view.
John Twilley Conservation Scientist
On Mon, 28 Jan 2008 19:46:21 -0500, {august04-at-verizon.net} wrote:
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I agree with Dale. You should change your own filters, if possible. I have seen the dual in-line filter and switching setups used in plants that he described. Get filters with a pressure relief button to make changing cartridges easier.
The real questions are, "What is plugging the (cartridge?) filter and what is the size of the 'dirt'? Once you know the ID, from TEM for example, then you might be able to find the source or cause.
McMaster-Carr shows a fiberglass unit very similar to a water softener. It is a 'sediment and dirt' filter unit with a back wash timer. It is item 9843K11, ~$400. It says, "Rated at 50 microns."
You could try to rent one from a water softener company with the option to buy. If you are lucky, a large dealer will have softener units used in apartments for a short periods of time that are quite new and half the price. I did that to get a year old iron filter that used KMnO4. I also bought a used water softener that had the resin and sand changed out by the dealer. Both worked just fine.
Paul
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Question: Does anyone happen to know the going price/value of a 14 year old Zeiss EM900 electron microscope? (My facility is planning to purchase a CCD camera for this particular scope, but then 2 or 3 years down the road, is planning on purchasing an entirely new scope altogether. In my humble opinion, it would make more sense to buy the new scope now, complete with the digital camera, so as not to try to retro-fit a camera we buy now for the Zeiss, onto a scope that may not be a Zeiss.) Thanks in advance for your time and perspectives. Rita Kenner
Hi Listers I am doing an analysis of some immuno-TEM. I am interested in the enrichment of gold particles on a membrane. I am probing a transmembrane protein with an IgG and then a secondary (gold labeled) IgG. What I am thinking is to count the gold particles that are within X nm of the membranes in question and the gold that is not within X distance of the membrane. Then define enrichment as;
(# gold within X nm of membrane)/(length of membrane * (2 * X)) __________________________________________________
(# gold particles in field)/(area of field)
My questions are; 1) does this make sense? 2) am I reinventing the wheel (I assume yes :-) 3) if 'yes' to #2, where should I go for info 4) (most important of all) what is the value of X?
Thank you David
==============================Original Headers============================== 6, 20 -- From Elliott-at-arizona.edu Tue Jan 29 06:32:29 2008 6, 20 -- Received: from smtpgate.email.arizona.edu (gandalf.email.Arizona.EDU [128.196.133.169]) 6, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0TCWSXR008708 6, 20 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 29 Jan 2008 06:32:29 -0600 6, 20 -- Received: from gandalfs_amavis (amavis1.email.arizona.edu [10.0.0.204]) 6, 20 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 3F75D3D8D1E 6, 20 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 29 Jan 2008 05:32:26 -0700 (MST) 6, 20 -- Received: from [192.168.0.30] (doctorelliott.us [70.57.226.104]) 6, 20 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 7A9293D8D1C 6, 20 -- for {Microscopy-at-MSA.Microscopy.Com} ; Tue, 29 Jan 2008 05:32:25 -0700 (MST) 6, 20 -- Message-Id: {BCDC9C19-A0F9-483B-A11B-C62646340EBB-at-arizona.edu} 6, 20 -- From: David Elliott {Elliott-at-arizona.edu} 6, 20 -- To: Microscopy ListServer {Microscopy-at-MSA.Microscopy.Com} 6, 20 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 6, 20 -- Content-Transfer-Encoding: 7bit 6, 20 -- Mime-Version: 1.0 (Apple Message framework v915) 6, 20 -- Subject: imuno-gold TEM analysis 6, 20 -- Date: Tue, 29 Jan 2008 05:32:19 -0700 6, 20 -- X-Mailer: Apple Mail (2.915) 6, 20 -- X-Virus-Scanned: amavisd-new at email.arizona.edu ==============================End of - Headers==============================
Any "scientific" light microscope will suffice. Just look at a suspension of bacteria at 40x or 100x objective (at 40x the bacteria are just very tiny spots but you can see them moving) and you will see them dancing around. If flagella are involved in the movement, they have a faster and more regular movement like turning in circles or rushing fast through the field of view. By extension, any particle with a size close to bacteria can be visualized as well, like the debris usually present in eucaryotic cell cultures, fine dusts...
Best regards,
Stephane
----- Original Message ---- X-from: "august04-at-verizon.net" {august04-at-verizon.net} To: nizets2-at-yahoo.com Sent: Tuesday, January 29, 2008 1:51:29 AM
This Question was submitted to Ask-A-Microscopist by (august04-at-verizon.net) from http://www.msa.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Monday, January 28, 2008 at 18:36:19 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both august04-at-verizon.net as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Email: august04-at-verizon.net Name: Charles Pique
Organization: None
Education: Graduate College
Location: Charleston, West Virginia, USA
Title: Brownian motion
Question: What magnification and conditions are needed to see the brownian motion?
==============================Original Headers============================== 9, 11 -- From zaluzec-at-ultra5.microscopy.com Mon Jan 28 18:46:05 2008 9, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 9, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0T0k4im016746 9, 11 -- for {microscopy-at-microscopy.com} ; Mon, 28 Jan 2008 18:46:05 -0600 9, 11 -- Mime-Version: 1.0 9, 11 -- Message-Id: {p06240801c3c427b741b0-at-[206.69.208.22]} 9, 11 -- Date: Mon, 28 Jan 2008 18:46:02 -0600 9, 11 -- To: microscopy-at-microscopy.com 9, 11 -- From: august04-at-verizon.net (by way of Ask-A-Microscopist) 9, 11 -- Subject: AskAMicroscopist: Brownian motion 9, 11 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers==============================
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==============================Original Headers============================== 22, 20 -- From nizets2-at-yahoo.com Tue Jan 29 07:48:50 2008 22, 20 -- Received: from web37407.mail.mud.yahoo.com (web37407.mail.mud.yahoo.com [209.191.91.139]) 22, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m0TDmosY024424 22, 20 -- for {microscopy-at-microscopy.com} ; Tue, 29 Jan 2008 07:48:50 -0600 22, 20 -- Received: (qmail 49491 invoked by uid 60001); 29 Jan 2008 13:48:50 -0000 22, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 22, 20 -- s=s1024; d=yahoo.com; 22, 20 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:Cc:MIME-Version:Content-Type:Message-ID; 22, 20 -- b=p7QLK/e7CXBp28SfC+cZrP3dtEsqnA0oM4wN9QcHL2YVcNa7kRcb+438Msy4sySyy21Bq8uqsuoYqWcd0rLTAwRtvK72Vn/rrX07VdJvU5gA3S+FaIhq0RkkkVS/f4xU9kD/ziFTRfSWs09xEILF/WX2/FIKbjX/gDJXkLex2To=; 22, 20 -- X-YMail-OSG: QdrD8V8VM1kb.qt_dur1QAiTJg_dMJ0WoeLWb7gY31xTxyaGDxAu9j_NlREIV1KIlNnSy0tVI7f7XYGHAj9UrNiftc7CsObCi_99yUHc57mkvRQ2W7N_Ped7r9nm5ZFadJ1yaxfXY9evarG99.sgeC2hWZDfLuZYp2vQV6TU1Lkf 22, 20 -- Received: from [80.122.101.100] by web37407.mail.mud.yahoo.com via HTTP; Tue, 29 Jan 2008 05:48:50 PST 22, 20 -- X-Mailer: YahooMailRC/818.31 YahooMailWebService/0.7.160 22, 20 -- Date: Tue, 29 Jan 2008 05:48:50 -0800 (PST) 22, 20 -- From: Stephane Nizet {nizets2-at-yahoo.com} 22, 20 -- Subject: Re: [Microscopy] AskAMicroscopist: Brownian motion 22, 20 -- To: august04-at-verizon.net 22, 20 -- Cc: microscopy-at-microscopy.com 22, 20 -- MIME-Version: 1.0 22, 20 -- Content-Type: text/plain; charset=us-ascii 22, 20 -- Message-ID: {324504.48968.qm-at-web37407.mail.mud.yahoo.com} ==============================End of - Headers==============================
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Email: tjzhang-at-umd.edu Name: Tim
Organization: UMD
Title-Subject: [Filtered] Help please: ESEM-E3
Question: Dear All,
There is an ESEM E3 in our lab that is over 15 years old but runs well.
Last week, I found the battery on CPU board runs out. I have ordered the battery on line but I need to re-load the "start software". I wonder who has this kind of software and how to send it to RAM memory on CUP board?
There were some recent postings regarding service and maintenance of older Ultracut and Ultracut E microtomes. I didn't see any responses providing resources for after-market service in the Midwest area, and so I'm raising the question again. Is there anyone reasonably near the Chicago area who provides service for both ambient and cryo units?
Thanks in advance,
Elaine
Elaine Schumacher Senior Research Scientist McCrone Associates, Inc. 850 Pasquinelli Drive Westmont,IL 60559-5539 USA 630-887-7100 (tel) 630-887-7417 (fax) E-mail: eschumacher-at-mccrone.com Web Site: www.mccrone.com
==============================Original Headers============================== 9, 27 -- From eschumacher-at-mccrone.com Tue Jan 29 08:46:00 2008 9, 27 -- Received: from pgp.mccrone.com (65-43-97-126.ded.ameritech.net [65.43.97.126]) 9, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0TEk0A7007180 9, 27 -- for {microscopy-at-microscopy.com} ; Tue, 29 Jan 2008 08:46:00 -0600 9, 27 -- Received: from pgp.mccrone.com (localhost [127.0.0.1]) 9, 27 -- by pgp.mccrone.com (Postfix) with ESMTP id 675481A8015 9, 27 -- for {microscopy-at-microscopy.com} ; Tue, 29 Jan 2008 08:46:01 -0600 (CST) 9, 27 -- Received: from MCCRONEMSG.tmg.mccrone.com ([192.168.101.20]) 9, 27 -- by pgp.mccrone.com (PGP Universal service); 9, 27 -- Tue, 29 Jan 2008 08:46:01 -0600 9, 27 -- X-PGP-Universal: processed 9, 27 -- Content-class: urn:content-classes:message 9, 27 -- MIME-Version: 1.0 9, 27 -- Content-Type: text/plain; 9, 27 -- charset="US-ASCII" 9, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 9, 27 -- Subject: After-Market Service for Ultracut Microtomes 9, 27 -- Date: Tue, 29 Jan 2008 08:45:54 -0600 9, 27 -- Message-ID: {2A27CE0EC2A5C748974EA513DFB285A70150DC04-at-MCCRONEMSG.tmg.mccrone.com} 9, 27 -- X-MS-Has-Attach: 9, 27 -- X-MS-TNEF-Correlator: 9, 27 -- Thread-Topic: After-Market Service for Ultracut Microtomes 9, 27 -- Thread-Index: AchihaZa3fymcB7KR+ye8rnYL7E12A== 9, 27 -- From: "Elaine F. Schumacher" {eschumacher-at-mccrone.com} 9, 27 -- To: {microscopy-at-microscopy.com} 9, 27 -- Content-Transfer-Encoding: 8bit 9, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m0TEk0A7007180 ==============================End of - Headers==============================
Hi Elaine, I use David Benjamin {dbenjamin-at-dbmicroservice.com} . He is based in the Atlanta area so this info may not seem helpful but Airtran has many direct flights to your area. He's a good serviceman for microtomes, cryostats, and microscopes.
best, Beth
On Jan 29, 2008, at 9:46 AM, eschumacher-at-mccrone.com wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Greetings Fellow Microscopists, } } There were some recent postings regarding service and maintenance of } older Ultracut and Ultracut E microtomes. I didn't see any responses } providing resources for after-market service in the Midwest area, } and so } I'm raising the question again. Is there anyone reasonably near the } Chicago area who provides service for both ambient and cryo units? } } Thanks in advance, } } Elaine } } } Elaine Schumacher } Senior Research Scientist } McCrone Associates, Inc. } 850 Pasquinelli Drive } Westmont,IL 60559-5539 USA } 630-887-7100 (tel) } 630-887-7417 (fax) } E-mail: eschumacher-at-mccrone.com } Web Site: www.mccrone.com } } } } } ==============================Original } Headers============================== } 9, 27 -- From eschumacher-at-mccrone.com Tue Jan 29 08:46:00 2008 } 9, 27 -- Received: from pgp.mccrone.com } (65-43-97-126.ded.ameritech.net [65.43.97.126]) } 9, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id m0TEk0A7007180 } 9, 27 -- for {microscopy-at-microscopy.com} ; Tue, 29 Jan 2008 08:46:00 } -0600 } 9, 27 -- Received: from pgp.mccrone.com (localhost [127.0.0.1]) } 9, 27 -- by pgp.mccrone.com (Postfix) with ESMTP id 675481A8015 } 9, 27 -- for {microscopy-at-microscopy.com} ; Tue, 29 Jan 2008 08:46:01 } -0600 (CST) } 9, 27 -- Received: from MCCRONEMSG.tmg.mccrone.com ([192.168.101.20]) } 9, 27 -- by pgp.mccrone.com (PGP Universal service); } 9, 27 -- Tue, 29 Jan 2008 08:46:01 -0600 } 9, 27 -- X-PGP-Universal: processed } 9, 27 -- Content-class: urn:content-classes:message } 9, 27 -- MIME-Version: 1.0 } 9, 27 -- Content-Type: text/plain; } 9, 27 -- charset="US-ASCII" } 9, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 } 9, 27 -- Subject: After-Market Service for Ultracut Microtomes } 9, 27 -- Date: Tue, 29 Jan 2008 08:45:54 -0600 } 9, 27 -- Message-ID: {2A27CE0EC2A5C748974EA513DFB285A70150DC04-at-MCCRONEMSG.tmg.mccrone.com } } } 9, 27 -- X-MS-Has-Attach: } 9, 27 -- X-MS-TNEF-Correlator: } 9, 27 -- Thread-Topic: After-Market Service for Ultracut Microtomes } 9, 27 -- Thread-Index: AchihaZa3fymcB7KR+ye8rnYL7E12A== } 9, 27 -- From: "Elaine F. Schumacher" {eschumacher-at-mccrone.com} } 9, 27 -- To: {microscopy-at-microscopy.com} } 9, 27 -- Content-Transfer-Encoding: 8bit } 9, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id m0TEk0A7007180 } ==============================End of - } Headers==============================
==============================Original Headers============================== 6, 21 -- From beth-at-plantbio.uga.edu Tue Jan 29 09:25:25 2008 6, 21 -- Received: from dogwood.plantbio.uga.edu (dogwood.plantbio.uga.edu [128.192.26.2]) 6, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0TFPOAA000611 6, 21 -- for {microscopy-at-microscopy.com} ; Tue, 29 Jan 2008 09:25:25 -0600 6, 21 -- Received: from [128.192.26.46] ([128.192.26.46]) 6, 21 -- (authenticated user beth-at-plantbio.uga.edu) 6, 21 -- by dogwood.plantbio.uga.edu 6, 21 -- (using TLSv1/SSLv3 with cipher AES128-SHA (128 bits)); 6, 21 -- Tue, 29 Jan 2008 10:25:18 -0500 6, 21 -- Cc: microscopy microscopy {microscopy-at-microscopy.com} 6, 21 -- Message-Id: {EE74570B-F994-4E80-ADD5-9DC10280F656-at-plantbio.uga.edu} 6, 21 -- From: Beth Richardson {beth-at-plantbio.uga.edu} 6, 21 -- To: eschumacher-at-mccrone.com 6, 21 -- In-Reply-To: {200801291446.m0TEkbsK008814-at-ns.microscopy.com} 6, 21 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 6, 21 -- Content-Transfer-Encoding: 7bit 6, 21 -- Mime-Version: 1.0 (Apple Message framework v915) 6, 21 -- Subject: Re: [Microscopy] After-Market Service for Ultracut Microtomes 6, 21 -- Date: Tue, 29 Jan 2008 10:26:27 -0500 6, 21 -- References: {200801291446.m0TEkbsK008814-at-ns.microscopy.com} 6, 21 -- X-Mailer: Apple Mail (2.915) ==============================End of - Headers==============================
I've got a surgeon who is working with human livers that are very hard from the embalming process and he had heard that microwaving tissue may soften up the tissue and make it easier to cut. I'm going to let him use my Biowave and try different wattages. I have never heard of this effect before. Has anybody out there heard of or actually know if hardened fixed tissue can be softened by microwaves. Thanks, all help is appreciated.
Tom Bargar University of Nebraska Medical Center Core Electron Microscopy Research Facility 986395 Nebraska Medical Center Omaha, NE 68198-6395 402-559-7347 tbargar-at-unmc.edu
==============================Original Headers============================== 4, 20 -- From tbargar-at-unmc.edu Tue Jan 29 09:29:00 2008 4, 20 -- Received: from zixvpm01.unmc.edu (zixvpm01.unmc.edu [192.198.54.126]) 4, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0TFT028004622 4, 20 -- for {Microscopy-at-MSA.Microscopy.com} ; Tue, 29 Jan 2008 09:29:00 -0600 4, 20 -- Received: from zixvpm01.unmc.edu (ZixVPM [127.0.0.1]) 4, 20 -- by Outbound.unmc.edu (Proprietary) with ESMTP id 2FE894C084 4, 20 -- for {Microscopy-at-MSA.Microscopy.com} ; Tue, 29 Jan 2008 09:29:00 -0600 (CST) 4, 20 -- Received: from unmcnotes01.unmc.edu (host-137-197-103-35.unmc.edu [137.197.103.35]) 4, 20 -- by zixvpm01.unmc.edu (Proprietary) with ESMTP id C51AF4C06E 4, 20 -- for {Microscopy-at-MSA.Microscopy.com} ; Tue, 29 Jan 2008 09:28:58 -0600 (CST) 4, 20 -- Subject: Can hardened fixed tissue be "softened" by Microwave treatment? 4, 20 -- To: Microscopy-at-MSA.Microscopy.com 4, 20 -- X-Mailer: Lotus Notes Release 7.0.1 January 17, 2006 4, 20 -- Message-ID: {OF0B63F2D6.945FCCD8-ON862573DF.005434C9-862573DF.00550C8B-at-unmc.edu} 4, 20 -- From: Tom W Bargar {tbargar-at-unmc.edu} 4, 20 -- Date: Tue, 29 Jan 2008 09:28:57 -0600 4, 20 -- X-MIMETrack: Serialize by Router on UNMCNOTES01.UNMC.EDU/Servers/UNEBR at 01/29/2008 09:28:58 4, 20 -- AM 4, 20 -- MIME-Version: 1.0 4, 20 -- Content-type: text/plain; charset=US-ASCII ==============================End of - Headers==============================
Brownian motion is readily demonstrated using small particles in an aqueous phase. For example, India ink (consisting of carbon black particles) can be observed under high magnification (500X and above) to see the random movement of the black particles. The particles seem to "shiver" or "quake" and sometimes move off in random directions. If you have phase contrast (or darkfield), this would enhance the viewing. If not, then try offsetting the substage condenser so that the light is coming in at an angle.
John J. Bozzola, Ph.D., Director Integrated Microscopy & Graphics Expertise (IMAGE) Southern Illinois University 750 Communications Drive - MC 4402 Carbondale, IL 62901 Telephone: 618-453-3730
I am interested in buying a zeiss axioplan microscope, (late 80's early 90's) or later model axiotron if available. If anyone has one they would like to sell or know of a source I would greatly appreciate a reply. Off line is probably best. Cheers Pat
Patrick R Kelly Operations Manager Geotrack International Pty Ltd ABN16 006 821 209 37 Melville Road, Brunswick West, Victoria 3055 Australia Telephone: +613 93801077 Facsimile: +613 93801477 email: mail-at-geotrack.com.au web: http://www.geotrack.com.au
==============================Original Headers============================== 4, 24 -- From probe-at-geotrack.com.au Wed Jan 30 00:47:28 2008 4, 24 -- Received: from geotrack.com.au (mail.geotrack.com.au [203.222.101.142]) 4, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0U6lRLJ023731 4, 24 -- for {microscopy-at-microscopy.com} ; Wed, 30 Jan 2008 00:47:28 -0600 4, 24 -- Received: from PAT (pat.geotrack.com.au [192.168.5.18]) 4, 24 -- by geotrack.com.au (Postfix) with SMTP id 8FFFB1C00151 4, 24 -- for {microscopy-at-microscopy.com} ; Wed, 30 Jan 2008 17:47:11 +1100 (EST) 4, 24 -- Message-ID: {004201c8630b$fa2dba30$1205a8c0-at-geotrack.com.au} 4, 24 -- Reply-To: "Pat Kelly" {probe-at-geotrack.com.au} 4, 24 -- From: "Pat Kelly" {probe-at-geotrack.com.au} 4, 24 -- To: {microscopy-at-microscopy.com} 4, 24 -- Subject: zeiss axioplan microscope 4, 24 -- Date: Wed, 30 Jan 2008 17:47:27 +1100 4, 24 -- Organization: Geotrack International 4, 24 -- MIME-Version: 1.0 4, 24 -- Content-Type: text/plain; 4, 24 -- format=flowed; 4, 24 -- charset="iso-8859-1"; 4, 24 -- reply-type=original 4, 24 -- Content-Transfer-Encoding: 7bit 4, 24 -- X-Priority: 3 4, 24 -- X-MSMail-Priority: Normal 4, 24 -- X-Mailer: Microsoft Outlook Express 6.00.2900.3138 4, 24 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 ==============================End of - Headers==============================
I wish to purchase a flatbed scanner for TEM negatives. I have received recommendations for the Canon 9950F and the Epson V750-M. The former may no longer be available, as it is not listed on the Canon web site--only the 8800F is. Any suggestions would be welcome and highly appreciated. Vachik Hacopian
==============================Original Headers============================== 2, 18 -- From vhacopia-at-firstclass.wellesley.edu Wed Jan 30 07:36:09 2008 2, 18 -- Received: from cliff.wellesley.edu (cliff.wellesley.edu [149.130.13.51]) 2, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0UDa95N022047 2, 18 -- for {Microscopy-at-Microscopy.Com} ; Wed, 30 Jan 2008 07:36:09 -0600 2, 18 -- Received: from firstclass.wellesley.edu (firstclass.wellesley.edu [149.130.13.40]) 2, 18 -- by cliff.wellesley.edu (8.13.8/8.13.8) with ESMTP id m0UDa8Jp027778 2, 18 -- for {Microscopy-at-Microscopy.Com} ; Wed, 30 Jan 2008 08:36:08 -0500 (EST) 2, 18 -- (envelope-from vhacopia-at-firstclass.wellesley.edu) 2, 18 -- Message-id: {fc.006640d8188521f4006640d8188521f4.18859c62-at-firstclass.wellesley.edu} 2, 18 -- Date: Wed, 30 Jan 2008 08:36:05 -0500 2, 18 -- Subject: TEM - negative scanners 2, 18 -- X-Mailer: FirstClass 9.0 (build 8.949) 2, 18 -- X-FC-SERVER-TZ: 15729388 2, 18 -- To: Microscopy-at-Microscopy.Com 2, 18 -- From: "Vachik Hacopian" {vhacopian-at-wellesley.edu} 2, 18 -- MIME-Version: 1.0 2, 18 -- Content-Type: text/plain; charset=UTF-8 2, 18 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
We just changed the IGP pump of the gun chamber only 4 years after the installation of our Tecnai G20. This change was advised by a FEI engineer. We do not even have 400 working hours on this machine and I wondered if it was normal that we already have to change the pump. We work mainly with ultrathin sections of biological probes embedded in Epon. They are pretty contaminated with carbon, as evidenced by light circles remaining on the sections after even a very short illumination time (I am usually working at 120 kV, LaB6). I am wondering if this carbon contamination, evaporated by the electron beam, is not at least partly responsible for the dirtiness of the pumps (the pump of the column is bigger than the one for the gun, which would explain why it was not so dirty). If this is the case, perhaps a plasma cleaner would not only be a convenience for me but could bring a financial advantage for my boss (I know you see what I mean ;-)). What would be the cost of a plasma cleaner? What is your opinion on the question? Do you think that a plasma cleaner would increase the lifetime of the IGPs?
Best regards,
Stephane
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==============================Original Headers============================== 6, 18 -- From nizets2-at-yahoo.com Wed Jan 30 09:00:54 2008 6, 18 -- Received: from web37402.mail.mud.yahoo.com (web37402.mail.mud.yahoo.com [209.191.91.134]) 6, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m0UF0pGt005637 6, 18 -- for {microscopy-at-microscopy.com} ; Wed, 30 Jan 2008 09:00:53 -0600 6, 18 -- Received: (qmail 99976 invoked by uid 60001); 30 Jan 2008 15:00:50 -0000 6, 18 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 6, 18 -- s=s1024; d=yahoo.com; 6, 18 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:To:MIME-Version:Content-Type:Message-ID; 6, 18 -- b=GhQVB0xX2vcU83GfwvaZw+ifHUliA8IiLQHTZzdx3l1eUcyH0/480eo0thBMxgf3UWIzP9wOTjIMLyE3Vcuk+F+LF9WdARAeAKeteL2+M8tArqzjD+Ms3FCyb8Qutj6M6tE0fcvz6TzcAreJXfnLKDhyAOWq+S20/9pUAEHFJq8=; 6, 18 -- X-YMail-OSG: OzrDwaUVM1lgyKl4Xyckx0JtBXwejlTzTn4PS5.ANAmKzTTfIP7HLGPXkbnY_W4GzDVe.Mr3IfmhgAzdLAg6vS83QQMBWKgdGjifx9pPsyBVYWmv4Mk- 6, 18 -- Received: from [80.122.101.100] by web37402.mail.mud.yahoo.com via HTTP; Wed, 30 Jan 2008 07:00:50 PST 6, 18 -- X-Mailer: YahooMailRC/818.31 YahooMailWebService/0.7.162 6, 18 -- Date: Wed, 30 Jan 2008 07:00:50 -0800 (PST) 6, 18 -- From: Stephane Nizet {nizets2-at-yahoo.com} 6, 18 -- To: microscopy-at-microscopy.com 6, 18 -- MIME-Version: 1.0 6, 18 -- Content-Type: text/plain; charset=us-ascii 6, 18 -- Message-ID: {440079.99572.qm-at-web37402.mail.mud.yahoo.com} ==============================End of - Headers==============================
Postdoctoral position at Northwestern University DFT and Electron Microscopy
A postdoctoral position is available at Northwestern University for someone with expertise in both DFT (Wien2k, Vasp, PWSCF) and transmission electron microscopy. The research will be in a number of different areas including charge density measurements at both surfaces and for bulk materials; structure and energies of surface reconstructions; oxides for use either as catalysts or in solid oxide fuel cells. The applicant must hold a recent Ph.D. in physics, chemistry, or materials science. Further requirements for this position are: (1) A good knowledge of electronic structure theory. (2) Experience with first-principles calculations such as Density Functional Theory. (3) Extensive hands-on experience with transmission electron microscopy and diffraction (4) Ability to communicate effectively with coworkers and collaborators. (5) Demonstrated ability to write high-quality manuscripts suitable for publication in peer-reviewed journals.
-- Laurence Marks Department of Materials Science and Engineering MSE Rm 2036 Cook Hall 2220 N Campus Drive Northwestern University Evanston, IL 60208, USA Tel: (847) 491-3996 Fax: (847) 491-7820 email: L-marks at northwestern dot edu Web: www.numis.northwestern.edu EMM2007 http://ns.crys.ras.ru/EMMM07/ Commission on Electron Diffraction of IUCR www.numis.northwestern.edu/IUCR_CED
==============================Original Headers============================== 4, 27 -- From marksmsa-at-gmail.com Wed Jan 30 12:46:52 2008 4, 27 -- Received: from el-out-1112.google.com (el-out-1112.google.com [209.85.162.181]) 4, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0UIkpuv025139 4, 27 -- for {Microscopy-at-microscopy.com} ; Wed, 30 Jan 2008 12:46:51 -0600 4, 27 -- Received: by el-out-1112.google.com with SMTP id z25so110171ele.4 4, 27 -- for {Microscopy-at-microscopy.com} ; Wed, 30 Jan 2008 10:46:51 -0800 (PST) 4, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 27 -- d=gmail.com; s=gamma; 4, 27 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 4, 27 -- bh=VUPAIlevAYLo1qi15Tl9L4WgCnc923pGS7B9+XqMxdk=; 4, 27 -- b=b1xIIDcQclQCLwHMmJEymrfonRl5c0gXDr0SiX17t0Tet1dsOnh3lRPM0Xj3+t0DeF9+pHD/1UjVsR7ZrAy0PGs24lGbf3RO2OfIIVulahCI5IcK1qlpVDIVTg2D/UOej3J+Pi0/655XR8PzUhDEI4grr6G1Crm/6wNBAudrPNs= 4, 27 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 4, 27 -- d=gmail.com; s=gamma; 4, 27 -- h=message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 4, 27 -- b=sX80fu+LLdH9157vrLZ9s3lfmJH/alUKBwXrdvhWdvKHh1ZdZ4W6prQPQt9C6Sb0ytDCrc4Nu/aNpxU7Pp1UOQ3oZZ/S6i+z25ohFrxiofIKgkeiscO46hKEVrtkQffRLlTRXFR1kscSSKZEwr4PjJC/1aOMZVEsRpqydcjmwEw= 4, 27 -- Received: by 10.142.84.3 with SMTP id h3mr584001wfb.34.1201718810259; 4, 27 -- Wed, 30 Jan 2008 10:46:50 -0800 (PST) 4, 27 -- Received: by 10.142.223.6 with HTTP; Wed, 30 Jan 2008 10:46:50 -0800 (PST) 4, 27 -- Message-ID: {e13ba6260801301046s104dacfhdc92b1b01dff683a-at-mail.gmail.com} 4, 27 -- Date: Wed, 30 Jan 2008 12:46:50 -0600 4, 27 -- From: "L Marks" {marksmsa-at-gmail.com} 4, 27 -- To: Microscopy-at-microscopy.com 4, 27 -- Subject: Postdoctoral Position 4, 27 -- MIME-Version: 1.0 4, 27 -- Content-Type: text/plain; charset=ISO-8859-1 4, 27 -- Content-Transfer-Encoding: 7bit 4, 27 -- Content-Disposition: inline ==============================End of - Headers==============================
Postdoctoral position at Northwestern University In-Situ Tribology at the Nanoscale
A postdoctoral position is available at Northwestern University for someone with expertise in mechanical properties at the nanoscale and transmission electron microscopy, and the ability to do materials modelling. The research will involve extending established dislocation and deformation models used for grain boundaries and interfaces in strong materials to weak interfaces relevant to tribological applications [1-3]. For instance, the work might involve testing the limits of applying conventional models for restricting the motion of dislocations due to point defects in the bulk to the problem of point defects at the interface of two sliding materials. The applicant must hold a recent Ph.D. in physics, chemistry, or materials science with a preference for materials science. Further requirements for this position are: (1) A good knowledge basic mechanical properties at the atomistic nanoscale. (2) Some experience with transmission electron microscopy and diffraction in order to collaborate with others doing more experimental work 3) Ability to communicate effectively with coworkers and collaborators. (5) Demonstrated ability to write high-quality manuscripts suitable for publication in peer-reviewed journals.
Interested applicants should contact L-marks -at- northwestern.edu and send a CV including the names of referees as well as a 1 page outline of how they might use core materials science mechanical property formalism's and theories to model tribological processes at the nanoscale?
1. A. P. Merkle and L. D. Marks, Tribology Letters 26 73 (2007) 2. A. P. Merkle and L. D. Marks, Philosophical Magazine Letters, 87, 527 (2007) 3. A. P. Merkal and L. D. Marks, Appl Phys Letts 90, 064101 (2007)
-- Laurence Marks Department of Materials Science and Engineering MSE Rm 2036 Cook Hall 2220 N Campus Drive Northwestern University Evanston, IL 60208, USA Tel: (847) 491-3996 Fax: (847) 491-7820 email: L-marks at northwestern dot edu Web: www.numis.northwestern.edu EMM2007 http://ns.crys.ras.ru/EMMM07/ Commission on Electron Diffraction of IUCR www.numis.northwestern.edu/IUCR_CED
==============================Original Headers============================== 5, 27 -- From marksmsa-at-gmail.com Wed Jan 30 12:56:47 2008 5, 27 -- Received: from el-out-1112.google.com (el-out-1112.google.com [209.85.162.176]) 5, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0UIulsO005392 5, 27 -- for {microscopy-at-microscopy.com} ; Wed, 30 Jan 2008 12:56:47 -0600 5, 27 -- Received: by el-out-1112.google.com with SMTP id z25so113806ele.4 5, 27 -- for {microscopy-at-microscopy.com} ; Wed, 30 Jan 2008 10:56:47 -0800 (PST) 5, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 5, 27 -- d=gmail.com; s=gamma; 5, 27 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 5, 27 -- bh=AI4O1eDs9HFTvWG0x2fklgT3b0p52FmTtm3slMXB0G0=; 5, 27 -- b=sYmAb8FlZAgtM2iDoYIlg0u/nSWcnjqUThppTCkPeBvAdEFI6i9MdWev1SUn0dRpCSRgdAmjuzpb9NNpsioM/G9Zkw9hUM5sSVXyi5ElJkpGu+ZtCzXsET2QrCOFQ96aFlGoOB6o+zC4P7Rpy6XSnbPXsGko6KoQqlGwrbC7MMA= 5, 27 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 5, 27 -- d=gmail.com; s=gamma; 5, 27 -- h=message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 5, 27 -- b=vjbXqtQW/s8pfdARrDbsPtv4rbAfDE6WXe84dK3IDN9YLze5oZR9BE+x1yNDP3IWYipgXA9Tffa9/fWnyScQAizgq1zk2HhQYMJqhgO4kcBlbqkyKvr5FneTHnNOez0K0z5Gpq12WDgy0n3UPb9AHbm0I0xZjmh7FwJ0V4z8OvE= 5, 27 -- Received: by 10.142.246.8 with SMTP id t8mr600001wfh.8.1201719405750; 5, 27 -- Wed, 30 Jan 2008 10:56:45 -0800 (PST) 5, 27 -- Received: by 10.142.223.6 with HTTP; Wed, 30 Jan 2008 10:56:45 -0800 (PST) 5, 27 -- Message-ID: {e13ba6260801301056p6a7f29aas55134f1887f588e6-at-mail.gmail.com} 5, 27 -- Date: Wed, 30 Jan 2008 12:56:45 -0600 5, 27 -- From: "L Marks" {marksmsa-at-gmail.com} 5, 27 -- To: microscopy {microscopy-at-microscopy.com} 5, 27 -- Subject: Postdoctoral Position in Nanoscale Tribology 5, 27 -- MIME-Version: 1.0 5, 27 -- Content-Type: text/plain; charset=ISO-8859-1 5, 27 -- Content-Transfer-Encoding: 7bit 5, 27 -- Content-Disposition: inline ==============================End of - Headers==============================
Here are the leads that were suggested for possible servicing of older Ultracut microtomes. You may find some of them to be useful for other types of equipment service as well.
Tek-Net, Jon Petz, Lakewood, NJ, 732-905-5530 They send a crate for shipment of the equipment, which you return to them when you get the repaired unit back.
David Benjamin, dbenjamin-at-dbmicroservice.com, Atlanta, GA
Sercomp International, www.sercompintl.com
Please note that I haven't investigated any of these resources yet for my specific Ultracut issues, but I wanted to share the information. Thanks very much to all who replied to my posting.
Regards,
Elaine
Elaine Schumacher Senior Research Scientist McCrone Associates, Inc. 850 Pasquinelli Drive Westmont,IL 60559-5539 USA 630-887-7100 (tel) 630-887-7417 (fax) E-mail: eschumacher-at-mccrone.com Web Site: www.mccrone.com
==============================Original Headers============================== 11, 27 -- From eschumacher-at-mccrone.com Wed Jan 30 13:05:12 2008 11, 27 -- Received: from pgp.mccrone.com (65-43-97-126.ded.ameritech.net [65.43.97.126]) 11, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0UJ5CcO018299 11, 27 -- for {microscopy-at-microscopy.com} ; Wed, 30 Jan 2008 13:05:12 -0600 11, 27 -- Received: from pgp.mccrone.com (localhost [127.0.0.1]) 11, 27 -- by pgp.mccrone.com (Postfix) with ESMTP id CE45A1A8013 11, 27 -- for {microscopy-at-microscopy.com} ; Wed, 30 Jan 2008 13:05:12 -0600 (CST) 11, 27 -- Received: from MCCRONEMSG.tmg.mccrone.com ([192.168.101.20]) 11, 27 -- by pgp.mccrone.com (PGP Universal service); 11, 27 -- Wed, 30 Jan 2008 13:05:12 -0600 11, 27 -- X-PGP-Universal: processed 11, 27 -- Content-class: urn:content-classes:message 11, 27 -- MIME-Version: 1.0 11, 27 -- Content-Type: text/plain; 11, 27 -- charset="US-ASCII" 11, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 11, 27 -- Subject: Summary of Suggestions for Microtome Service 11, 27 -- Date: Wed, 30 Jan 2008 13:05:06 -0600 11, 27 -- Message-ID: {2A27CE0EC2A5C748974EA513DFB285A70150DC14-at-MCCRONEMSG.tmg.mccrone.com} 11, 27 -- X-MS-Has-Attach: 11, 27 -- X-MS-TNEF-Correlator: 11, 27 -- Thread-Topic: Summary of Suggestions for Microtome Service 11, 27 -- Thread-Index: AchjcwalgYTpq17cQSK1HknLSVnvxg== 11, 27 -- From: "Elaine F. Schumacher" {eschumacher-at-mccrone.com} 11, 27 -- To: {microscopy-at-microscopy.com} 11, 27 -- Content-Transfer-Encoding: 8bit 11, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m0UJ5CcO018299 ==============================End of - Headers==============================
On Jan 30, 2008, at 7:01 AM, nizets2-at-yahoo.com wrote:
} We just changed the IGP pump of the gun chamber only 4 years after } the installation of our Tecnai G20. This change was advised by a } FEI engineer. } We do not even have 400 working hours on this machine and I } wondered if it was normal that we already have to change the pump. } We work mainly with ultrathin sections of biological probes } embedded in Epon. They are pretty contaminated with carbon, as } evidenced by light circles remaining on the sections after even a } very short illumination time (I am usually working at 120 kV, } LaB6). I am wondering if this carbon contamination, evaporated by } the electron beam, is not at least partly responsible for the } dirtiness of the pumps (the pump of the column is bigger than the } one for the gun, which would explain why it was not so dirty). If } this is the case, perhaps a plasma cleaner would not only be a } convenience for me but could bring a financial advantage for my } boss (I know you see what I mean ;-)). } What would be the cost of a plasma cleaner? } What is your opinion on the question? Do you think that a plasma } cleaner would increase the lifetime of the IGPs?
Dear Stephane, The IGP is on full time, so even though there have been few hours that the instrument was in use, the pump has been working for the entire 4 years. This is still a short period, especially since there are relatively few contaminants in the gun volume (or should be). The mechanism of ion pumps is that residual gas is ionized and the ions are accelerated by an electric field and become embedded in the getter, which can get filled to the extent that any additional ion incident on the getter will dislodge an ion that is already there. The lifetime, therefore, depends on how good the vacuum is and how large the surface of the getter is. Other things that can shorten pump lifetime are the presence of water in the column--which is not likely to be an issue for your usage and is more of a problem in cryoEM--and the formation of spurs on the getter surface. The indication for pump replacement is loss of performance, so if your vacuum is poor, where it had been good, then the pump should be replaced (assuming that a vacuum leak has not developed). I doubt that carbon is the problem, but I could be wrong about that. A plasma cleaner can remove contamination from the column, which will give you a cleaner instrument and a better vacuum, which, in turn, will increase pump lifetime, but I don't know whether that will be enough to cover the cost--I'll let the manufacturers of these systems answer that question. Yours, Bill Tivol, PhD EM Scientist Electron Cryo-Microscopy Facility Broad Center, Mail Code 114-96 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 6, 22 -- From tivol-at-caltech.edu Wed Jan 30 14:17:14 2008 6, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0UKHEBK002305 6, 22 -- for {microscopy-at-msa.microscopy.com} ; Wed, 30 Jan 2008 14:17:14 -0600 6, 22 -- Received: from earth-dog.its.caltech.edu (earth-dog [192.168.1.3]) 6, 22 -- by water-ox-postvirus (Postfix) with ESMTP id 0C1E21BAB8 6, 22 -- for {microscopy-at-msa.microscopy.com} ; Wed, 30 Jan 2008 12:17:02 -0800 (PST) 6, 22 -- Received: from [192.168.159.158] (jpix-01.caltech.edu [131.215.2.133]) 6, 22 -- by water-ox.its.caltech.edu (Postfix) with ESMTP id 0E7951BB11 6, 22 -- for {microscopy-at-msa.microscopy.com} ; Wed, 30 Jan 2008 12:16:59 -0800 (PST) 6, 22 -- Mime-Version: 1.0 (Apple Message framework v753) 6, 22 -- In-Reply-To: {200801301501.m0UF17Bd005785-at-ns.microscopy.com} 6, 22 -- References: {200801301501.m0UF17Bd005785-at-ns.microscopy.com} 6, 22 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 6, 22 -- Message-Id: {94B58638-19FD-4442-A214-C1F9CFDB21DC-at-caltech.edu} 6, 22 -- Content-Transfer-Encoding: 7bit 6, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 6, 22 -- Subject: Re: [Microscopy] 6, 22 -- Date: Wed, 30 Jan 2008 12:17:06 -0800 6, 22 -- To: microscopy-at-msa.microscopy.com 6, 22 -- X-Mailer: Apple Mail (2.753) 6, 22 -- X-Spam-Scanned: at Caltech-ITS on earth-dog by amavisd-2.4.5 ==============================End of - Headers==============================
I've been following this discussion with interest since I have never had an instrument with IGP's. I'm surprised to find that IGP's have a (sometimes rather) limited lifetime. When you say they need to be replaced, do you mean just the adsorptive surfaces or the entire sealed system? How much does this cost? A ballpark figure is fine. I need this information for planning and teaching purposes.
Thank you,
John Bozzola Integrated Microscopy & Graphics Expertise (IMAGE) Southern Illinois University 750 Communications Drive - MC 4402 Carbondale, IL 62901 Telephone: 618-453-3730
==============================Original Headers============================== 4, 19 -- From bozzola-at-siu.edu Wed Jan 30 14:50:02 2008 4, 19 -- Received: from cstmta3.siu.edu (cstmta3.siu.edu [131.230.1.3]) 4, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0UKo2r9015938 4, 19 -- for {Microscopy-at-msa.microscopy.com} ; Wed, 30 Jan 2008 14:50:02 -0600 4, 19 -- Received: from [131.230.177.136] (ws177136.microscope.siu.edu [131.230.177.136]) 4, 19 -- by cstmta3.siu.edu (Switch-3.3.0/Switch-3.3.0) with ESMTP id m0UKo0xV010337 4, 19 -- for {Microscopy-at-msa.microscopy.com} ; Wed, 30 Jan 2008 14:50:01 -0600 (CST) 4, 19 -- Mime-Version: 1.0 4, 19 -- Message-Id: {a06240809c3c691fcef3f-at-[131.230.177.136]} 4, 19 -- In-Reply-To: {200801302018.m0UKIL32003821-at-ns.microscopy.com} 4, 19 -- References: {200801302018.m0UKIL32003821-at-ns.microscopy.com} 4, 19 -- Date: Wed, 30 Jan 2008 14:49:57 -0600 4, 19 -- To: Microscopy-at-msa.microscopy.com 4, 19 -- From: "John J. Bozzola" {bozzola-at-siu.edu} 4, 19 -- Subject: [Microscopy] Re: ion pump longevity 4, 19 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 4, 19 -- X-Spam-Score: 0.00% 4, 19 -- X-MASF: 0.00% 4, 19 -- X-Whitelist: 0.00% ==============================End of - Headers==============================
Not seeing a discussion of bakeout, I'd bring that up.
New generation pumps have built-in bakeout coils and instrument vacuum connections also have a bakeout coil surrounding each one. These pumps are essential for high vacuum that is highly beneficial for LaB6 cathodes and especially critical for FE systems. For W systems, I have never seen an IGP unless it was set up to handle both W and LaB6.
Depending on the use of the system, eventually the gun chamber will become contaminated with gas and always does when the cathode or FE tip is changed. Part of the changeout process is to bakeout the gun chamber, IGPs and vacuum connections (the connections with a gazillion bolts like between IGP and column/chamber). During this time, isolation valves that seal off the column and the gun chamber are opened while baking so the main vacuum system pulls the junk out. When done, the valves are closed and IGPs start pulling the vacuum down further.
During the bakeout process, the junk in the Ta and Ti parts of the IGP also get dislodged and dumped. This can be a separate part of "recycling" an IGP that is staturated but not requiring repair. When the IGP needs repair/rebuild, the whole thing (minus magnet) is sent off for rebuild or exchange. The following list from Duniway gives some typical costs for this.
I have not seen prices or services for the newer IGPs.
Older tools that do not have bakeout coils built-in can still be baked using off the shelf heating coil tape. They are rated in watts, width and length and are controlled by a simple thermal feedback box. These units are typically around $500 or less and can save lots of bucks if a simple bakeout is all that is needed from time to time--and with cathode/filament change.
With bakeout when the gun is changed and with reasonable care, my experience is that IGPs last for many years...perhaps up to ten years of continuous service. But of course, there is always the flakey one that does develop a flake and trips the IGP power supply and must be replaced.
gary g.
At 12:51 PM 1/30/2008, you wrote:
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==============================Original Headers============================== 17, 20 -- From gary-at-gaugler.com Wed Jan 30 15:24:50 2008 17, 20 -- Received: from smtp1.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 17, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m0ULOonG029294 17, 20 -- for {microscopy-at-microscopy.com} ; Wed, 30 Jan 2008 15:24:50 -0600 17, 20 -- Message-Id: {200801302124.m0ULOonG029294-at-ns.microscopy.com} 17, 20 -- Received: (qmail 32761 invoked from network); 30 Jan 2008 13:24:49 -0800 17, 20 -- Received: by simscan 1.1.0 ppid: 32758, pid: 32759, t: 0.1345s 17, 20 -- scanners: regex: 1.1.0 attach: 1.1.0 17, 20 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 17, 20 -- by smtp1 with SMTP; 30 Jan 2008 13:24:49 -0800 17, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 17, 20 -- Date: Wed, 30 Jan 2008 13:24:48 -0800 17, 20 -- To: bozzola-at-siu.edu 17, 20 -- From: Gary Gaugler {gary-at-gaugler.com} 17, 20 -- Subject: Re: [Microscopy] Re: ion pump longevity 17, 20 -- Cc: MSA listserver {microscopy-at-microscopy.com} 17, 20 -- In-Reply-To: {200801302051.m0UKpVjW018590-at-ns.microscopy.com} 17, 20 -- References: {200801302051.m0UKpVjW018590-at-ns.microscopy.com} 17, 20 -- Mime-Version: 1.0 17, 20 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-72B419C3 ==============================End of - Headers==============================
On Jan 30, 2008, at 12:50 PM, bozzola-at-siu.edu wrote:
} I've been following this discussion with interest since I have never } had an instrument with IGP's. I'm surprised to find that IGP's have a } (sometimes rather) limited lifetime. When you say they need to be } replaced, do you mean just the adsorptive surfaces or the entire } sealed system? How much does this cost? A ballpark figure is fine. I } need this information for planning and teaching purposes.
Dear John, For the ion pumps I've dealt with, the getter assembly is replaced. This is the plates and whatever holds them at the proper separation. I've sent the pump back to the EM manufacturer and gotten a refurbished pump back. I have had only very limited experience with this, however, because the ion pumps on the HVEM were very large with correspondingly large capacity and didn't need replacing for the entire 20+ years I was in Albany, and the pump in our FEG here at Caltech was covered by the service contract. Apparently it developed a whisker on the getter, the symptoms of which were that the vacuum would get bad fairly quickly and shut the FEG off. This happened every few months until the problem was diagnosed. Yours, Bill Tivol, PhD EM Scientist Electron Cryo-Microscopy Facility Broad Center, Mail Code 114-96 California Institute of Technology Pasadena CA 91125 (626) 395-8833 tivol-at-caltech.edu
==============================Original Headers============================== 6, 22 -- From tivol-at-caltech.edu Wed Jan 30 15:27:07 2008 6, 22 -- Received: from outgoing-mail.its.caltech.edu (outgoing-mail.its.caltech.edu [131.215.239.19]) 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0ULR50D032447 6, 22 -- for {microscopy-at-msa.microscopy.com} ; Wed, 30 Jan 2008 15:27:06 -0600 6, 22 -- Received: from water-dog.its.caltech.edu (water-dog [192.168.1.26]) 6, 22 -- by water-ox-postvirus (Postfix) with ESMTP id 154D61BAF1 6, 22 -- for {microscopy-at-msa.microscopy.com} ; Wed, 30 Jan 2008 13:27:04 -0800 (PST) 6, 22 -- Received: from [192.168.159.158] (jpix-01.caltech.edu [131.215.2.133]) 6, 22 -- by wood-ox.its.caltech.edu (Postfix) with ESMTP id CD64D13E3F 6, 22 -- for {microscopy-at-msa.microscopy.com} ; Wed, 30 Jan 2008 13:27:01 -0800 (PST) 6, 22 -- Mime-Version: 1.0 (Apple Message framework v753) 6, 22 -- In-Reply-To: {200801302050.m0UKoAts016075-at-ns.microscopy.com} 6, 22 -- References: {200801302050.m0UKoAts016075-at-ns.microscopy.com} 6, 22 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 6, 22 -- Message-Id: {6EFB292B-2DF8-4A94-BA51-F77952586F50-at-caltech.edu} 6, 22 -- Content-Transfer-Encoding: 7bit 6, 22 -- From: Bill Tivol {tivol-at-caltech.edu} 6, 22 -- Subject: Re: [Microscopy] Re: ion pump longevity 6, 22 -- Date: Wed, 30 Jan 2008 13:27:08 -0800 6, 22 -- To: microscopy-at-msa.microscopy.com 6, 22 -- X-Mailer: Apple Mail (2.753) 6, 22 -- X-Spam-Scanned: at Caltech-ITS on water-dog by amavisd-2.4.5 ==============================End of - Headers==============================
If the negtive is just slightly denser than "normal" the Canon scanner cannot give a good full tonal scan. The D-Max is insufficient. I have not tried the Epson Larry
vhacopian-at-wellesley.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I wish to purchase a flatbed scanner for TEM negatives. I have received } recommendations for the Canon 9950F and the Epson V750-M. The former may } no longer be available, as it is not listed on the Canon web site--only } the 8800F is. Any suggestions would be welcome and highly appreciated. } Vachik Hacopian } } } ==============================Original Headers============================== } 2, 18 -- From vhacopia-at-firstclass.wellesley.edu Wed Jan 30 07:36:09 2008 } 2, 18 -- Received: from cliff.wellesley.edu (cliff.wellesley.edu [149.130.13.51]) } 2, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0UDa95N022047 } 2, 18 -- for {Microscopy-at-Microscopy.Com} ; Wed, 30 Jan 2008 07:36:09 -0600 } 2, 18 -- Received: from firstclass.wellesley.edu (firstclass.wellesley.edu [149.130.13.40]) } 2, 18 -- by cliff.wellesley.edu (8.13.8/8.13.8) with ESMTP id m0UDa8Jp027778 } 2, 18 -- for {Microscopy-at-Microscopy.Com} ; Wed, 30 Jan 2008 08:36:08 -0500 (EST) } 2, 18 -- (envelope-from vhacopia-at-firstclass.wellesley.edu) } 2, 18 -- Message-id: {fc.006640d8188521f4006640d8188521f4.18859c62-at-firstclass.wellesley.edu} } 2, 18 -- Date: Wed, 30 Jan 2008 08:36:05 -0500 } 2, 18 -- Subject: TEM - negative scanners } 2, 18 -- X-Mailer: FirstClass 9.0 (build 8.949) } 2, 18 -- X-FC-SERVER-TZ: 15729388 } 2, 18 -- To: Microscopy-at-Microscopy.Com } 2, 18 -- From: "Vachik Hacopian" {vhacopian-at-wellesley.edu} } 2, 18 -- MIME-Version: 1.0 } 2, 18 -- Content-Type: text/plain; charset=UTF-8 } 2, 18 -- Content-Transfer-Encoding: 8bit } ==============================End of - Headers============================== }
-- Larry Ackerman, Associate Specialist UCSF, Dept. of Anatomy, Rm S1347 513 Parnassus Ave., Box 0452 San Francisco, CA 94143
larry.ackerman-at-ucsf.edu
==============================Original Headers============================== 5, 28 -- From Larry.Ackerman-at-ucsf.edu Wed Jan 30 16:07:40 2008 5, 28 -- Received: from emfmcb01.ucsfmedicalcenter.org (emfmcb01.ucsfmedicalcenter.org [64.54.46.97]) 5, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0UM7ewB023069 5, 28 -- for {Microscopy-at-microscopy.com} ; Wed, 30 Jan 2008 16:07:40 -0600 5, 28 -- Received: from 64.54.128.152 by emfmcb01.ucsfmedicalcenter.org with 5, 28 -- ESMTP (Tumbleweed Email Firewall SMTP Relay (Email Firewall v6.1.0)); 5, 28 -- Wed, 30 Jan 2008 14:10:41 -0800 5, 28 -- X-Server-Uuid: E2E48A14-EE5B-4280-A138-188440602EDD 5, 28 -- Received: from baluk.ucsf.edu ([128.218.123.88]) by exvs06.net.ucsf.edu 5, 28 -- with Microsoft SMTPSVC(6.0.3790.3959); Wed, 30 Jan 2008 14:07:33 -0800 5, 28 -- Message-ID: {47A0F525.4040408-at-ucsf.edu} 5, 28 -- Date: Wed, 30 Jan 2008 14:07:33 -0800 5, 28 -- From: "Larry Ackerman" {larry.ackerman-at-ucsf.edu} 5, 28 -- Reply-to: larry.ackerman-at-ucsf.edu 5, 28 -- Organization: UCSF, NeuroAnatomy 5, 28 -- User-Agent: Thunderbird 2.0.0.9 (Macintosh/20071031) 5, 28 -- MIME-Version: 1.0 5, 28 -- To: Microscopy-at-microscopy.com 5, 28 -- Subject: Re: [Microscopy] TEM - negative scanners 5, 28 -- References: {200801301347.m0UDlJv5001465-at-ns.microscopy.com} 5, 28 -- In-Reply-To: {200801301347.m0UDlJv5001465-at-ns.microscopy.com} 5, 28 -- X-OriginalArrivalTime: 30 Jan 2008 22:07:33.0413 (UTC) 5, 28 -- FILETIME=[8393C550:01C8638C] 5, 28 -- X-WSS-ID: 6BBE2A6B29O5772273-01-01 5, 28 -- Content-Type: text/plain; 5, 28 -- charset=iso-8859-1; 5, 28 -- format=flowed 5, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Title-Subject: [Filtered] 2 questions concerning microtomy of thin films
Question: I have a multi layer film in which one of the layers is water soluble. We want to cross section the sample for TEM to view the various layers, each of which are less than 500nm thick. The total thickness of the plastic substrate plus multilayer film is aprox. 500microns (the substate is aprox 300microns of this). I've embedded the film in a epoxy resin for sectioning. I don't know how to go about capturing thin sections ( {100nm) if I can't put water in the boat. I could cut them dry, but again, I'm not sure how to transfer them to the grid. I am using a Diatome diamond knife. Any suggestions would be greatly appreciated.
Another question I have regards the use of tape as a support media for cryo-sectioning. Many of our samples are films which I typically embed in epoxy resin. We now have cryo-sectioning capabilities and i would like to simply sandwich my film between tape and cryo cut thin films for TEM analyses. Is there any tape which has good adhesion properties at -120C and stand up well for TEM (glue does not interfere with sample)
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Email: hadden-at-wingate.edu Name: Lee Hadden
Organization: Wingate University
Title-Subject: [Filtered] vacuum problems JEOL JSM5600LV SEM
Question: Our JEOL 5600lv SEM does not attain operating vacuum. It goes through the PRE-EVAC and into the EVAC modes in normal time frame, but remains in EVAC, never going to READY for specimen observation. Any ideas for fixing the problem? Cooling water temp and flow rate OK as are DP temp and oil. The column will hold a vacuum for weeks + even with the SEM off.
It has been a week since my posting. I received 7 replies.
All replies were in favor of smaller dishes with one stating that 96 wells would be even better for his work.
I do not think that this is a large enough group of potential customers for the any company to consider it a valid request to make such a product.
The reason that I did not like the chamber slides was that there was so little flat area when I embedded the cells in a 4 well configuration. I also forgot to use LX-112 (Ladd) in my Epon formula instead of EM-Bed812 (Electron Microscopy Sciences) so the sides of the wells melted a bit making it very hard to get the sides off the polymerized Epon blocks. Perhaps I'll try removing the sides before embedding the next time and use the slide duplicating mold to hold the Epon onto the Permanox slide for curing or get some 2 well chambered slides.
One person, Stephane, asked where I got the 60mm dishes. My supplier is Electron Microscopy Sciences but I believe other suppliers may also carry them.
Thank you for participating. Pat
} For quite some time I have wondered why NUNC did not make a Permanox Petri } Dish smaller than 60 x 15 mm. I have grown to really appreciate the } advantages of using the Permanox over standard Polystyrene dishes for tissue } cultured cells grown for TEM experiments since every cell line that I have } tried adheres well to them, they can withstand chemicals like acetone and } propylene oxide that dissolve the polystyrene and the embedded cells come } away from the Permanox so easily and smoothly. } } With the special cells and reagents that I am now using for TEM, it is a big } waste to grow cells over such a large area when a 35 x 15 mm dish would do } nicely. I do want a dish not a chambered slide. } } I would like to know if there are others (you) } 1. who would switch to a smaller dish if they would be made available. } (this is my pick) } 2. who would like to use both the 60mm and 35mm dishes } 3. who would only use the 60mm dishes } If I get a reasonable response I will contact the company with my results to } back up my request that they consider making the smaller dishes. } } Comments are welcome. } } For the survey, it may be best to answer me "Off-ListServer" so as not to } fill up the emails of other members. I will let the ListServer know the } tally after the replies come in. } } Patricia Stranen Connelly } Biologist, Electron Microscopy } NHLBI Electron Microscopy Core } National Institutes of Health } 14 Service Road South } Bldg. 14E Rm. 111B MSC 5570 } Bethesda, MD 20892-5570 } Phone 301-496-3491 } FAX 301-480-6560 } connellyps-at-mail.nih.gov
==============================Original Headers============================== 9, 24 -- From connellyps-at-nhlbi.nih.gov Wed Jan 30 17:07:07 2008 9, 24 -- Received: from NIHCESSMTP3.hub.nih.gov (nihcessmtp3.hub.nih.gov [128.231.90.117]) 9, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0UN772Y029774 9, 24 -- for {microscopy-at-microscopy.com} ; Wed, 30 Jan 2008 17:07:07 -0600 9, 24 -- Received: from NIHCESMLBX6.nih.gov ([156.40.71.206]) by NIHCESSMTP3.hub.nih.gov with Microsoft SMTPSVC(6.0.3790.1830); 9, 24 -- Wed, 30 Jan 2008 18:07:02 -0500 9, 24 -- Received: from 156.40.71.188 ([156.40.71.188]) by NIHCESMLBX6.nih.gov ([156.40.71.206]) via Exchange Front-End Server mail.nih.gov ([156.40.71.169]) with Microsoft Exchange Server HTTP-DAV ; 9, 24 -- Wed, 30 Jan 2008 23:07:02 +0000 9, 24 -- User-Agent: Microsoft-Entourage/11.3.6.070618 9, 24 -- Date: Wed, 30 Jan 2008 18:04:26 -0500 9, 24 -- Subject: Re: [Microscopy] Interest in smaller "Permanox" dishes?-Results 9, 24 -- From: Patricia Connelly {connellyps-at-nhlbi.nih.gov} 9, 24 -- To: {connellyps-at-nhlbi.nih.gov} 9, 24 -- CC: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 9, 24 -- Message-ID: {C3C66CAA.1484%connellyps-at-nhlbi.nih.gov} 9, 24 -- Thread-Topic: [Microscopy] Interest in smaller "Permanox" dishes?-Results 9, 24 -- Thread-Index: AchjlHWjtAmjls+HEdyflQANk2Yv1A== 9, 24 -- In-Reply-To: {200801240003.m0O03d9H016406-at-ns.microscopy.com} 9, 24 -- Mime-version: 1.0 9, 24 -- Content-type: text/plain; 9, 24 -- charset="ISO-8859-1" 9, 24 -- X-OriginalArrivalTime: 30 Jan 2008 23:07:02.0666 (UTC) FILETIME=[D3048EA0:01C86394] 9, 24 -- Content-Transfer-Encoding: 8bit 9, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m0UN772Y029774 ==============================End of - Headers==============================
I also work with water soluble materials, and so often have to cut dry. I have found that a diamond knife is preferable to glass (less "sticky" so sections are easier to get off the knife surface), but that may be something you want to experiment with. To transfer sections from the knife to the grid, I use an eyelash probe (I buy mine from Ted Pella, but people make them as well). It takes a little practice, but that is what works best for me. I also use the probe to gently "flatten" the sections onto the grid surface (I use 300 mesh Copper grids with Formvar support film).
Diatome used to make a Cryo P diamond knife (don't know if they still do) with a special platform that makes it easier to transfer sections. This has been my favorite knife for dry-sectioning.
Sorry can't help with your second question, but hope this was useful.
Jessica
____________________ Jessica Cervantes Bend Research Inc 64550 Research Rd Bend, OR 97701 www.bendres.com
-----Original Message----- X-from: sandra.gardner-at-xerox.com [mailto:sandra.gardner-at-xerox.com] Sent: Wednesday, January 30, 2008 2:49 PM To: Cervantes, Jessica
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html ------------------------------------------------------------------------ --- Remember this posting is most likely not from a Subscriber, so when replying please copy both sandra.gardner-at-xerox.com as well as the MIcroscopy Listserver ------------------------------------------------------------------------ ---
Title-Subject: [Filtered] 2 questions concerning microtomy of thin films
Question: I have a multi layer film in which one of the layers is water soluble. We want to cross section the sample for TEM to view the various layers, each of which are less than 500nm thick. The total thickness of the plastic substrate plus multilayer film is aprox. 500microns (the substate is aprox 300microns of this). I've embedded the film in a epoxy resin for sectioning. I don't know how to go about capturing thin sections ( {100nm) if I can't put water in the boat. I could cut them dry, but again, I'm not sure how to transfer them to the grid. I am using a Diatome diamond knife. Any suggestions would be greatly appreciated.
Another question I have regards the use of tape as a support media for cryo-sectioning. Many of our samples are films which I typically embed in epoxy resin. We now have cryo-sectioning capabilities and i would like to simply sandwich my film between tape and cryo cut thin films for TEM analyses. Is there any tape which has good adhesion properties at -120C and stand up well for TEM (glue does not interfere with sample)
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both fvillalovoz-at-deltacollege.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: fvillalovoz-at-deltacollege.edu Name: Frank Villalovoz
Question: San Joaquin Delta College is currently seeking candidates for a full-time faculty position in electron microscopy with experience in the biological sciences. The successful candidate should have a strong background in sample preparation for both the TEM and SEM. The position requires individual training of students in microscope operation, ultramicrotomy, critical point drying and all related biological techniques. Teaching responsibilities also include light and electron optics, digital imaging, and other aspects of microscopy as needed. Degree requirements and salary are dependent upon experience.
San Joaquin Delta College in Stockton, California has a two-year training program that has been training students for almost 40 years. It is one of only two programs in the nation at the community college level. We currently have four SEMs, three TEMs, one FIB, a new AFM, and a compliment of biological and materials sample preparation instrumentation. The facility has 18,000 square feet.
Applications and a job description can be obtained at http://www.deltacollege.edu
Application information can be obtained from Jackie Layman, Human Resources, email: jlayman-at-deltacollege.edu (209) 954-5058
For additional information about the laboratory contact:
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Email: ramshes-at-musc.edu Name: Venkat Ramshesh
Organization: Medical University of South Carolina
Title-Subject: [Filtered] Announcement: LIGHT MICROSCOPY FOR THE BIOSCIENCES (LMB)
Question: Second Charleston Workshop on
LIGHT MICROSCOPY FOR THE BIOSCIENCES (LMB)
Medical University of South Carolina
May 18-23, 2008
The Second Charleston Workshop on LIGHT MICROSCOPY FOR THE BIOSCIENCES (LMB) Workshop provides a solid introduction to the concepts and practical applications of light microscopy relevant to modern cell and molecular biology. Students will have opportunities for extensive hands-on experience with state-of-the-art equipment for optical imaging,digital image processing, and fluorescence and confocal/multiphoton microscopy guided by experienced academic and commercial faculty. Lectures and laboratory exercises will include: optics of image formation; microscope alignment; phase contrast and differential interference contrast microscopy; video and digital cameras; contrast enhancement by analog and digital image processing; principles of fluorescence and fluorescence microscopy; ion imaging and fluorescent probes, including green fluorescent protein; fluorescence resonance energy transfer; and laser scanning confocal and multiphoton microscopy. A commercial faculty representing leading microscope manufacturers will make available for student use the latest and most advanced instrumentation for light microscopy, image detection and computerized image analysis. The LMB Workshop is designed for doctoral level scientists, advanced pre-doctoral students and high level technical personnel. No prior experience with microscopy is required. All students will benefit from in-depth interaction with instructors. Students are encouraged to bring their own specimens for analysis.
Tuition: $750.00
Application Deadline: April 1, 2008
Principal Instructors:
John J. Lemasters, M.D., Ph.D., Organizer
P. Darwin Bell, Ph.D.
Prakash Kara, Ph.D.
Margaret Kelly, Ph.D.
Peter Komlosi, Ph.D.
Anna-Liisa Nieminen, Ph.D.
Venkat Ramshesh, Ph.D.
To apply, send a curriculum vita and a brief letter describing your research interests and reasons for enrolling. Because the course is expected to be oversubscribed, applicants should inquire as soon as possible. Please indicate your complete mailing address, telephone/fax number and email address. Full consideration will be given to applications received by April 1, 2008.
For further information or to apply, contact:
Venkat K Ramshesh Medical University of South Carolina Center for Cell Death, Injury and Regeneration and Hollings Cancer Center 280 Calhoun Street, PO Box 250140 Charleston, SC 29425 Telephone (843) 792- 3530, FAX (843) 792-1617 E-mail: ramshes-at-musc.edu
A few cents too : as other have point out, the two main points are :
-at what vacuum levels has the pump worked ? If it works allways at -7 mbar or less, it will be earlier tired, by sputtering it Ti, building a conductive film on it insultors (leakage current, that means than even with a very good vacumm, one has a ion current from microamps to tenth of microamps), memory effects (giving pressure bursts which may shut off the gun, mmmmmmh !), and slow pumping rate. If it has worked at better than -9 mb, it will give good and faithfull service 20 years long. -It needs absolutly to be baked each time it has seen atomspheric pressure, to remove water vapor which limits its pumping cabability, as in each vacuum sytem. A lite bake is processed at 150-250 °C, a much stronger bakeout is done at 300°C. Above 300°C (up to 450 - 700°C) magnets must be removed, but this is more a refurbishment procedure than a maintenance one.
A aspect which is less known, at maybe this is your problem, is that hydrocarbons may paralyse the pumping process. Under the ion current of the pump and the e-beam of a TEM/SEM, one may polymerise them, generating a wide familly of carbon products. These can be a real poison for a ion pump. It's a known issue that one cannot put a ion pump on a vaccum vessel fororganics evaporation (only diff, turbo or cryo). One see always with a mass analyser a strong outgazing of CH4, CO, CO2 and much heavier spices, at the starting or at the shutt off of the HV of the pump, but in these cases much more. We have all seen the same problem with Penning gauges, which are a modified design of the ion pump (measuring without pumping, or pumping without measuring, different design and material choice !).
In the case of a TEM/SEM, one have diff pumps, more or less good traped, rotary vane pumps, more or les good traped too, and in your case, "carbon" samples, which outgas carbon spices. Do you have a LN2 trap near your sample stage ? Is a column vane between the sample stage and the gun ? What is the "normal" vaccum in the gun, and is it measured by a gauge, or the ion pump current ? This last point can give false diagnostic : one think the pump isn't good anymore, and the vacuum level is low, and in fact one have a good vacumm, but a wrong measuremnt, distored by the leakage current of the pump. And one changes the pump ! In that case, the real point to verify is how does the vacumm go down, dynamically, compared with the "normal" state.
I've more experience with ion pumps on clean UHV vessels than on TEMs, but our TEM (Topcon 02B, with Lab6) has the first pump, which begin to be now a bit tired after 15 years of work. Of coarse, we work on materials, not on biological samples.
Hope it helps
J. Faerber IPCMS-GSI (Institut de Physique et Chimie des Matériaux de Strasbourg Groupe Surface et Interfaces) 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
nizets2-at-yahoo.com a écrit : } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear listers, } } We just changed the IGP pump of the gun chamber only 4 years after the installation of our Tecnai G20. This change was advised by a FEI engineer. } We do not even have 400 working hours on this machine and I wondered if it was normal that we already have to change the pump. } We work mainly with ultrathin sections of biological probes embedded in Epon. They are pretty contaminated with carbon, as evidenced by light circles remaining on the sections after even a very short illumination time (I am usually working at 120 kV, LaB6). I am wondering if this carbon contamination, evaporated by the electron beam, is not at least partly responsible for the dirtiness of the pumps (the pump of the column is bigger than the one for the gun, which would explain why it was not so dirty). If this is the case, perhaps a plasma cleaner would not only be a convenience for me but could bring a financial advantage for my boss (I know you see what I mean ;-)). } What would be the cost of a plasma cleaner? } What is your opinion on the question? Do you think that a plasma cleaner would increase the lifetime of the IGPs? } } Best regards, } } Stephane } } } ____________________________________________________________________________________ } Looking for last minute shopping deals? } Find them fast with Yahoo! Search. http://tools.search.yahoo.com/newsearch/category.php?category=shopping } } ==============================Original Headers============================== } 6, 18 -- From nizets2-at-yahoo.com Wed Jan 30 09:00:54 2008 } 6, 18 -- Received: from web37402.mail.mud.yahoo.com (web37402.mail.mud.yahoo.com [209.191.91.134]) } 6, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m0UF0pGt005637 } 6, 18 -- for {microscopy-at-microscopy.com} ; Wed, 30 Jan 2008 09:00:53 -0600 } 6, 18 -- Received: (qmail 99976 invoked by uid 60001); 30 Jan 2008 15:00:50 -0000 } 6, 18 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; } 6, 18 -- s=s1024; d=yahoo.com; } 6, 18 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:To:MIME-Version:Content-Type:Message-ID; } 6, 18 -- b=GhQVB0xX2vcU83GfwvaZw+ifHUliA8IiLQHTZzdx3l1eUcyH0/480eo0thBMxgf3UWIzP9wOTjIMLyE3Vcuk+F+LF9WdARAeAKeteL2+M8tArqzjD+Ms3FCyb8Qutj6M6tE0fcvz6TzcAreJXfnLKDhyAOWq+S20/9pUAEHFJq8=; } 6, 18 -- X-YMail-OSG: OzrDwaUVM1lgyKl4Xyckx0JtBXwejlTzTn4PS5.ANAmKzTTfIP7HLGPXkbnY_W4GzDVe.Mr3IfmhgAzdLAg6vS83QQMBWKgdGjifx9pPsyBVYWmv4Mk- } 6, 18 -- Received: from [80.122.101.100] by web37402.mail.mud.yahoo.com via HTTP; Wed, 30 Jan 2008 07:00:50 PST } 6, 18 -- X-Mailer: YahooMailRC/818.31 YahooMailWebService/0.7.162 } 6, 18 -- Date: Wed, 30 Jan 2008 07:00:50 -0800 (PST) } 6, 18 -- From: Stephane Nizet {nizets2-at-yahoo.com} } 6, 18 -- To: microscopy-at-microscopy.com } 6, 18 -- MIME-Version: 1.0 } 6, 18 -- Content-Type: text/plain; charset=us-ascii } 6, 18 -- Message-ID: {440079.99572.qm-at-web37402.mail.mud.yahoo.com} } ==============================End of - Headers============================== }
==============================Original Headers============================== 12, 29 -- From jacques.faerber-at-ipcms.u-strasbg.fr Thu Jan 31 03:18:52 2008 12, 29 -- Received: from mailhost.u-strasbg.fr (mailhost.u-strasbg.fr [130.79.200.152]) 12, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0V9Ioxc010602 12, 29 -- for {microscopy-at-microscopy.com} ; Thu, 31 Jan 2008 03:18:51 -0600 12, 29 -- Received: from ipcms.u-strasbg.fr (ipcms.u-strasbg.fr [130.79.210.2]) 12, 29 -- by mailhost.u-strasbg.fr (8.13.8/jtpda-5.5pre1) with ESMTP id m0V9IjcQ016757 12, 29 -- ; Thu, 31 Jan 2008 10:18:45 +0100 (CET) 12, 29 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr [130.79.152.3]) 12, 29 -- by ipcms.u-strasbg.fr (Postfix) with ESMTP id 7CF3C107C7C5; 12, 29 -- Thu, 31 Jan 2008 10:17:03 +0100 (CET) 12, 29 -- Message-ID: {47A19224.20306-at-ipcms.u-strasbg.fr} 12, 29 -- Date: Thu, 31 Jan 2008 10:17:24 +0100 12, 29 -- From: "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr} 12, 29 -- User-Agent: Thunderbird 1.5.0.14pre (X11/20071022) 12, 29 -- MIME-Version: 1.0 12, 29 -- To: nizets2-at-yahoo.com, Microscopy-at-microscopy.com 12, 29 -- Subject: Re: [Microscopy]ion pump longevity 12, 29 -- References: {200801301556.m0UFucA3015156-at-ns.microscopy.com} 12, 29 -- In-Reply-To: {200801301556.m0UFucA3015156-at-ns.microscopy.com} 12, 29 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 12, 29 -- Content-Transfer-Encoding: 8bit 12, 29 -- X-IPCMS-MailScanner: Found to be clean 12, 29 -- X-IPCMS-MailScanner-From: jacques.faerber-at-ipcms.u-strasbg.fr 12, 29 -- X-Greylist: Sender IP whitelisted, not delayed by milter-greylist-3.0 (mailhost.u-strasbg.fr [130.79.200.152]); Thu, 31 Jan 2008 10:18:45 +0100 (CET) 12, 29 -- X-Virus-Scanned: ClamAV 0.88.7/5619/Wed Jan 30 22:55:02 2008 on mr2.u-strasbg.fr 12, 29 -- X-Virus-Status: Clean 12, 29 -- X-Spam-Status: No, score=-0.1 required=5.0 tests=AWL autolearn=disabled 12, 29 -- version=3.1.8 12, 29 -- X-Spam-Checker-Version: SpamAssassin 3.1.8 (2007-02-13) on mr2.u-strasbg.fr ==============================End of - Headers==============================
Avoid the 9950F at all costs (I have one at home, and a few years ago it was the best available but the Twain ScanGear software is absolute rubbish - it can't even scan negatives to A4 and refuses to scan if film isn't in the holders, ie. an unusual size). Plus it has lower resolution than the Epson V750 pro - as does the older Epson 4990 so avoid that one as well.
The present best of the flatbed scanner bunch for large negatives and under £1,000 (assuming you don't want to go to £10,000+) is the £600 Epson V750 Pro. We use it here to scan slide tissue sections as well as TEM negatives - it's scan detail is noticeably better than our Nikon 1x objective and it has far more even illumination (removing the microscope condenser for the 1x objective makes microscope illumination very uneven). Naturally go to a 4x or above objective and the microscope then to wins hands down - but if you want general overviews of a tissue section the Epson V750 is fast and built to scan flat things. Scans of film negatives are generally better in the supplied holders (as the height of the film is right for focus).
In the rush to digital, film scanners are becoming a niche area, so we are lucky there's still a few decent choices at under £1,000 - plus these cheap scanners knock the socks off far more expensive scanners produced in the late 1990s (in terms of image quality/resolution rather than ultimate build quality anyway).
TEM negatives should last 500 years correctly archived so most users scan at around 1,200 dpi for working copies rather than trying to archive images scanned at the full 6,400 dpi resolution where the image size is massive enough to be impractical for PC archiving (actually scanning at 3,200 dpi with the V750 often produces similar detail to 6,400 dpi with most film, but that’s still a very large image size in TIF). Don't throw away the B&W TEM film after scanning, it should last up to 100 times longer than any CD-RW/DVD-RW disk.
So have a look at the V750 Pro - it has better optics and is a more versatile scanner than it's sibling V700 - although other extras like colour targets are more related to colour balance for scanning colour film, but worth having none the less. It scans up to an A4 negative, plus it can scan in A4 reflective mode as well, and the twain Scan software is pretty good. Only downside is the flimsy film holders - some buy new/spare ones* or have their workshop build something suitable if they are scanning film all day.
I can send you a copy of my article on the subject of scanning film [RMS InFocus & Microscopy Now] if you are interested.
Independent V750 review (as a colour film slide scanner) http://www.photo-i.co.uk/index.html http://www.photo-i.co.uk/Reviews/interactive/Epson%20V750/page_1.htm
*e.g. Doug Fishers film holders http://www.betterscanning.com/ http://www.photo-i.co.uk/Reviews/interactive/Epson%204870/DF_holder/MF.htm but may not suite TEM film sizes
Keith
-------------------------------------------------------------------------- Dr keith J. Morris Molecular Cytogenetics and Microscopy Core Laboratory 00/069 and 00/070 The Wellcome Trust Centre for Human Genetics Roosevelt Drive Oxford OX3 7BN United Kingdom Telephone: +44 (0)1865 287568 Email: kjmorris-at-well.ox.ac.uk Web-pages: http://www.well.ox.ac.uk/cytogenetics/
-----Original Message----- X-from: vhacopian-at-wellesley.edu [mailto:vhacopian-at-wellesley.edu] Sent: 30 January 2008 13:48 To: kjmorris-at-well.ox.ac.uk
I wish to purchase a flatbed scanner for TEM negatives. I have received recommendations for the Canon 9950F and the Epson V750-M. The former may no longer be available, as it is not listed on the Canon web site--only the 8800F is. Any suggestions would be welcome and highly appreciated. Vachik Hacopian
==============================Original Headers============================== 2, 18 -- From vhacopia-at-firstclass.wellesley.edu Wed Jan 30 07:36:09 2008 2, 18 -- Received: from cliff.wellesley.edu (cliff.wellesley.edu [149.130.13.51]) 2, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0UDa95N022047 2, 18 -- for {Microscopy-at-Microscopy.Com} ; Wed, 30 Jan 2008 07:36:09 -0600 2, 18 -- Received: from firstclass.wellesley.edu (firstclass.wellesley.edu [149.130.13.40]) 2, 18 -- by cliff.wellesley.edu (8.13.8/8.13.8) with ESMTP id m0UDa8Jp027778 2, 18 -- for {Microscopy-at-Microscopy.Com} ; Wed, 30 Jan 2008 08:36:08 -0500 (EST) 2, 18 -- (envelope-from vhacopia-at-firstclass.wellesley.edu) 2, 18 -- Message-id: {fc.006640d8188521f4006640d8188521f4.18859c62-at-firstclass.wellesley.edu} 2, 18 -- Date: Wed, 30 Jan 2008 08:36:05 -0500 2, 18 -- Subject: TEM - negative scanners 2, 18 -- X-Mailer: FirstClass 9.0 (build 8.949) 2, 18 -- X-FC-SERVER-TZ: 15729388 2, 18 -- To: Microscopy-at-Microscopy.Com 2, 18 -- From: "Vachik Hacopian" {vhacopian-at-wellesley.edu} 2, 18 -- MIME-Version: 1.0 2, 18 -- Content-Type: text/plain; charset=UTF-8 2, 18 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
==============================Original Headers============================== 22, 23 -- From kjmorris-at-well.ox.ac.uk Thu Jan 31 04:48:26 2008 22, 23 -- Received: from morse.well.ox.ac.uk (morse.well.ox.ac.uk [129.67.44.2]) 22, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0VAmQvl026363 22, 23 -- for {Microscopy-at-Microscopy.Com} ; Thu, 31 Jan 2008 04:48:26 -0600 22, 23 -- Received: from dhcp079.well.ox.ac.uk ([129.67.44.178] helo=CytoWhizz) 22, 23 -- by morse.well.ox.ac.uk with esmtp (Exim 4.52) 22, 23 -- id 1JKWy1-0002S4-Mu 22, 23 -- for Microscopy-at-Microscopy.Com; Thu, 31 Jan 2008 10:48:25 +0000 22, 23 -- From: "Keith Morris" {kjmorris-at-well.ox.ac.uk} 22, 23 -- To: {Microscopy-at-Microscopy.Com} 22, 23 -- References: {200801301347.m0UDlhHR001876-at-ns.microscopy.com} 22, 23 -- Subject: RE: [Microscopy] TEM - negative scanners 22, 23 -- Date: Thu, 31 Jan 2008 10:48:38 -0000 22, 23 -- Message-ID: {000901c863f6$d6aac6c0$b22c4381-at-CytoWhizz} 22, 23 -- MIME-Version: 1.0 22, 23 -- Content-Type: text/plain; 22, 23 -- charset="iso-8859-1" 22, 23 -- X-Mailer: Microsoft Office Outlook 11 22, 23 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 22, 23 -- Thread-Index: AchjRrEXp2t+N5l4QUO/MqsR+Ixv+AAqcVsg 22, 23 -- In-Reply-To: {200801301347.m0UDlhHR001876-at-ns.microscopy.com} 22, 23 -- Content-Transfer-Encoding: 8bit 22, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m0VAmQvl026363 ==============================End of - Headers==============================
The first step is to discover if the problem is related to poor ultimate vacuum or a bad gauge/controller. I have an independent vacuum gauge installed on my SEM for just this purpose.
Woody
Woody White, Electron Microscopist Babcock & Wicox Technical Services Group Lynchburg, VA
-----Original Message----- X-from: hadden-at-wingate.edu [mailto:hadden-at-wingate.edu] Sent: Wednesday, January 30, 2008 5:51 PM To: White, Woody N.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MicroscopyListserver/MLFormMail.html ------------------------------------------------------------------------ --- Remember this posting is most likely not from a Subscriber, so when replying please copy both hadden-at-wingate.edu as well as the MIcroscopy Listserver ------------------------------------------------------------------------ ---
Email: hadden-at-wingate.edu Name: Lee Hadden
Organization: Wingate University
Title-Subject: [Filtered] vacuum problems JEOL JSM5600LV SEM
Question: Our JEOL 5600lv SEM does not attain operating vacuum. It goes through the PRE-EVAC and into the EVAC modes in normal time frame, but remains in EVAC, never going to READY for specimen observation. Any ideas for fixing the problem? Cooling water temp and flow rate OK as are DP temp and oil. The column will hold a vacuum for weeks + even with the SEM off.
==============================Original Headers============================== 6, 11 -- From zaluzec-at-microscopy.com Wed Jan 30 16:41:28 2008 6, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 6, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0UMfO5N004394 6, 11 -- for {microscopy-at-microscopy.com} ; Wed, 30 Jan 2008 16:41:26 -0600 6, 11 -- Mime-Version: 1.0 6, 11 -- Message-Id: {p06240802c3c6ad86a149-at-[206.69.208.22]} 6, 11 -- Date: Wed, 30 Jan 2008 16:41:22 -0600 6, 11 -- To: microscopy-at-microscopy.com 6, 11 -- From: hadden-at-wingate.edu (by way of MicroscopyListserver) 6, 11 -- Subject: viaWWW: vacuum problems JEOL JSM5600LV SEM 6, 11 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers============================== ----------------------------------------- This message is intended only for the individual or entity to which it is addressed and contains information that is proprietary to The Babcock & Wilcox Company and/or its affiliates, or may be otherwise confidential. If the reader of this message is not the intended recipient, or the employee agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by return e-mail and delete this message from your computer. Thank you.
==============================Original Headers============================== 2, 28 -- From nwwhite-at-babcock.com Thu Jan 31 07:32:41 2008 2, 28 -- Received: from bwxt.com (lynsmtp1.bwxt.com [131.184.146.8]) 2, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0VDWegm012346 2, 28 -- for {microscopy-at-microscopy.com} ; Thu, 31 Jan 2008 07:32:40 -0600 2, 28 -- Received: from ([131.184.13.224]) 2, 28 -- by lynsmtp1.bwxt.com with ESMTP id 5202488.7568410; 2, 28 -- Thu, 31 Jan 2008 08:32:23 -0500 2, 28 -- Received: from BWXSPO01.BWXS.BWXTECH.NET ([131.184.13.31]) by bwxslynbh01.BWXS.BWXTECH.NET with Microsoft SMTPSVC(6.0.3790.3959); 2, 28 -- Thu, 31 Jan 2008 08:32:23 -0500 2, 28 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 2, 28 -- Content-class: urn:content-classes:message 2, 28 -- MIME-Version: 1.0 2, 28 -- Subject: RE: [Microscopy] viaWWW: vacuum problems JEOL JSM5600LV SEM 2, 28 -- Date: Thu, 31 Jan 2008 08:32:22 -0500 2, 28 -- Message-ID: {360DDAABED436B49BA397357CCEE8BB7F87A9B-at-BWXSPO01.BWXS.BWXTECH.NET} 2, 28 -- In-Reply-To: {200801302251.m0UMpLvu028306-at-ns.microscopy.com} 2, 28 -- X-MS-Has-Attach: 2, 28 -- X-MS-TNEF-Correlator: 2, 28 -- Thread-Topic: [Microscopy] viaWWW: vacuum problems JEOL JSM5600LV SEM 2, 28 -- Thread-Index: AchjkrpFG10KD302Rj+uTDfWedbYiwAenvcg 2, 28 -- References: {200801302251.m0UMpLvu028306-at-ns.microscopy.com} 2, 28 -- To: {hadden-at-wingate.edu} , {Microscopy-at-microscopy.com} 2, 28 -- X-OriginalArrivalTime: 31 Jan 2008 13:32:23.0279 (UTC) FILETIME=[B61D5FF0:01C8640D] 2, 28 -- From: "White, Woody N." {nwwhite-at-babcock.com} 2, 28 -- Content-Type: text/plain; 2, 28 -- charset="us-ascii" 2, 28 -- Content-Transfer-Encoding: 8bit 2, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m0VDWegm012346 ==============================End of - Headers==============================
Our unit (JEOL JSM 5800LV) had an identical problem. It would reach full vacuum, but not switch over to HT READY. This problem came all of a sudden; it didn't drift into the problem with pump times creeping into longer intervals. The fix for our unit was to adjust certain set points.
Our unit is under full service contract, so it was the manufacturer's service tech that repaired our problem. The fix was mentioned in the report... "adjusted TP 1 & 2 set points, TP1 set to 2.754, TP2 set to 1.584". I don't know what that specifically means, nor whether your unit has the same vacuum design. Maybe some of this information can help you.
} Name: Lee Hadden } } Organization: Wingate University } } Title-Subject: [Filtered] vacuum problems JEOL } JSM5600LV SEM } } Question: Our JEOL 5600lv SEM does not attain } operating vacuum. It goes through the PRE-EVAC and } into the EVAC modes in normal time frame, but } remains in EVAC, never going to READY for specimen } observation. Any ideas for fixing the problem? } Cooling water temp and flow rate OK as are DP temp } and oil. The column will hold a vacuum for weeks + } even with the SEM off. }
____________________________________________________________________________________ Looking for last minute shopping deals? Find them fast with Yahoo! Search. http://tools.search.yahoo.com/newsearch/category.php?category=shopping
==============================Original Headers============================== 7, 20 -- From smalinskas-at-yahoo.com Thu Jan 31 07:46:25 2008 7, 20 -- Received: from web34411.mail.mud.yahoo.com (web34411.mail.mud.yahoo.com [66.163.178.160]) 7, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m0VDkPX9025546 7, 20 -- for {microscopy-at-sparc5.microscopy.com} ; Thu, 31 Jan 2008 07:46:25 -0600 7, 20 -- Received: (qmail 44328 invoked by uid 60001); 31 Jan 2008 13:46:24 -0000 7, 20 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 7, 20 -- s=s1024; d=yahoo.com; 7, 20 -- h=X-YMail-OSG:Received:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding:Message-ID; 7, 20 -- b=W7LFUax5kc+I2B9hyx1yi7y2YumVI4BxzQx8trMQaJX8pTTg0FbmH0HnJrI/nQ2dqNNzB/214pFfoUKhWaTHQruLzDl5eHllKyWB6xQFr+u0VDB56Hfq9bg7F1GXsMSRxOi7wPikBUC78ktEGF4kx5JyZAdYmqGAiWblfUjBLZ8=; 7, 20 -- X-YMail-OSG: DBqSMvEVM1kQTMv9VBnBvhOZnpZNJlhGcqcvFGNOo1LWg7ufNSaIg1YiQl4a7ZmSGxoTnpQM6vrc3oSB4lMYfO6jEz0QfaViSvy0dPsPDH_013gvTbg- 7, 20 -- Received: from [141.151.33.213] by web34411.mail.mud.yahoo.com via HTTP; Thu, 31 Jan 2008 05:46:24 PST 7, 20 -- Date: Thu, 31 Jan 2008 05:46:24 -0800 (PST) 7, 20 -- From: Kestutis Smalinskas {smalinskas-at-yahoo.com} 7, 20 -- Subject: Re: [Microscopy] viaWWW: vacuum problems JEOL JSM5600LV SEM 7, 20 -- To: hadden-at-wingate.edu, microscopy-at-ns.microscopy.com 7, 20 -- In-Reply-To: {200801302243.m0UMh72r008475-at-ns.microscopy.com} 7, 20 -- MIME-Version: 1.0 7, 20 -- Content-Type: text/plain; charset=iso-8859-1 7, 20 -- Content-Transfer-Encoding: 8bit 7, 20 -- Message-ID: {732516.40379.qm-at-web34411.mail.mud.yahoo.com} ==============================End of - Headers==============================
I agree about the V750 pro being the current best in that price range. David
On Jan 31, 2008, at 3:52 AM, kjmorris-at-well.ox.ac.uk wrote:
} } } } ---------------------------------------------------------------------- } ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } Hi Vachik, } } Avoid the 9950F at all costs (I have one at home, and a few years } ago it was } the best available but the Twain ScanGear software is absolute } rubbish - it } can't even scan negatives to A4 and refuses to scan if film isn't } in the } holders, ie. an unusual size). Plus it has lower resolution than } the Epson } V750 pro - as does the older Epson 4990 so avoid that one as well. } } The present best of the flatbed scanner bunch for large negatives } and under } £1,000 (assuming you don't want to go to £10,000+) is the £600 } Epson V750 } Pro. We use it here to scan slide tissue sections as well as TEM } negatives - } it's scan detail is noticeably better than our Nikon 1x objective } and it has } far more even illumination (removing the microscope condenser for } the 1x } objective makes microscope illumination very uneven). Naturally go } to a 4x } or above objective and the microscope then to wins hands down - but } if you } want general overviews of a tissue section the Epson V750 is fast } and built } to scan flat things. Scans of film negatives are generally better } in the } supplied holders (as the height of the film is right for focus). } } In the rush to digital, film scanners are becoming a niche area, so } we are } lucky there's still a few decent choices at under £1,000 - plus } these cheap } scanners knock the socks off far more expensive scanners produced } in the } late 1990s (in terms of image quality/resolution rather than } ultimate build } quality anyway). } } TEM negatives should last 500 years correctly archived so most } users scan at } around 1,200 dpi for working copies rather than trying to archive } images } scanned at the full 6,400 dpi resolution where the image size is } massive } enough to be impractical for PC archiving (actually scanning at } 3,200 dpi } with the V750 often produces similar detail to 6,400 dpi with most } film, but } that’s still a very large image size in TIF). Don't throw away the } B&W TEM } film after scanning, it should last up to 100 times longer than any } CD-RW/DVD-RW disk. } } So have a look at the V750 Pro - it has better optics and is a more } versatile scanner than it's sibling V700 - although other extras } like colour } targets are more related to colour balance for scanning colour } film, but } worth having none the less. It scans up to an A4 negative, plus it } can scan } in A4 reflective mode as well, and the twain Scan software is } pretty good. } Only downside is the flimsy film holders - some buy new/spare ones* } or have } their workshop build something suitable if they are scanning film } all day. } } I can send you a copy of my article on the subject of scanning film } [RMS } InFocus & Microscopy Now] if you are interested. } } Independent V750 review (as a colour film slide scanner) } http://www.photo-i.co.uk/index.html } http://www.photo-i.co.uk/Reviews/interactive/Epson%20V750/page_1.htm } } *e.g. Doug Fishers film holders } http://www.betterscanning.com/ } http://www.photo-i.co.uk/Reviews/interactive/Epson%204870/DF_holder/ } MF.htm } but may not suite TEM film sizes } } Keith } } ---------------------------------------------------------------------- } ---- } Dr keith J. Morris } Molecular Cytogenetics and Microscopy Core } Laboratory 00/069 and 00/070 } The Wellcome Trust Centre for Human Genetics } Roosevelt Drive } Oxford } OX3 7BN } United Kingdom } Telephone: +44 (0)1865 287568 } Email: kjmorris-at-well.ox.ac.uk } Web-pages: http://www.well.ox.ac.uk/cytogenetics/ } } } } -----Original Message----- } X-from: vhacopian-at-wellesley.edu [mailto:vhacopian-at-wellesley.edu] } Sent: 30 January 2008 13:48 } To: kjmorris-at-well.ox.ac.uk } Subject: [Microscopy] TEM - negative scanners } } } } } ---------------------------------------------------------------------- } ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } I wish to purchase a flatbed scanner for TEM negatives. I have } received } recommendations for the Canon 9950F and the Epson V750-M. The } former may } no longer be available, as it is not listed on the Canon web site-- } only } the 8800F is. Any suggestions would be welcome and highly appreciated. } Vachik Hacopian } } } ==============================Original } Headers============================== } 2, 18 -- From vhacopia-at-firstclass.wellesley.edu Wed Jan 30 07:36:09 } 2008 } 2, 18 -- Received: from cliff.wellesley.edu (cliff.wellesley.edu } [149.130.13.51]) } 2, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } m0UDa95N022047 } 2, 18 -- for {Microscopy-at-Microscopy.Com} ; Wed, 30 Jan 2008 07:36:09 } -0600 } 2, 18 -- Received: from firstclass.wellesley.edu } (firstclass.wellesley.edu } [149.130.13.40]) } 2, 18 -- by cliff.wellesley.edu (8.13.8/8.13.8) with ESMTP id } m0UDa8Jp027778 } 2, 18 -- for {Microscopy-at-Microscopy.Com} ; Wed, 30 Jan 2008 08:36:08 } -0500 (EST) } 2, 18 -- (envelope-from vhacopia-at-firstclass.wellesley.edu) } 2, 18 -- Message-id: } {fc. } 006640d8188521f4006640d8188521f4.18859c62-at-firstclass.wellesley.edu} } 2, 18 -- Date: Wed, 30 Jan 2008 08:36:05 -0500 } 2, 18 -- Subject: TEM - negative scanners } 2, 18 -- X-Mailer: FirstClass 9.0 (build 8.949) } 2, 18 -- X-FC-SERVER-TZ: 15729388 } 2, 18 -- To: Microscopy-at-Microscopy.Com } 2, 18 -- From: "Vachik Hacopian" {vhacopian-at-wellesley.edu} } 2, 18 -- MIME-Version: 1.0 } 2, 18 -- Content-Type: text/plain; charset=UTF-8 } 2, 18 -- Content-Transfer-Encoding: 8bit } ==============================End of - } Headers============================== } } } } ==============================Original } Headers============================== } 22, 23 -- From kjmorris-at-well.ox.ac.uk Thu Jan 31 04:48:26 2008 } 22, 23 -- Received: from morse.well.ox.ac.uk (morse.well.ox.ac.uk } [129.67.44.2]) } 22, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id m0VAmQvl026363 } 22, 23 -- for {Microscopy-at-Microscopy.Com} ; Thu, 31 Jan 2008 } 04:48:26 -0600 } 22, 23 -- Received: from dhcp079.well.ox.ac.uk ([129.67.44.178] } helo=CytoWhizz) } 22, 23 -- by morse.well.ox.ac.uk with esmtp (Exim 4.52) } 22, 23 -- id 1JKWy1-0002S4-Mu } 22, 23 -- for Microscopy-at-Microscopy.Com; Thu, 31 Jan 2008 10:48:25 } +0000 } 22, 23 -- From: "Keith Morris" {kjmorris-at-well.ox.ac.uk} } 22, 23 -- To: {Microscopy-at-Microscopy.Com} } 22, 23 -- References: {200801301347.m0UDlhHR001876-at-ns.microscopy.com} } 22, 23 -- Subject: RE: [Microscopy] TEM - negative scanners } 22, 23 -- Date: Thu, 31 Jan 2008 10:48:38 -0000 } 22, 23 -- Message-ID: {000901c863f6$d6aac6c0$b22c4381-at-CytoWhizz} } 22, 23 -- MIME-Version: 1.0 } 22, 23 -- Content-Type: text/plain; } 22, 23 -- charset="iso-8859-1" } 22, 23 -- X-Mailer: Microsoft Office Outlook 11 } 22, 23 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 } 22, 23 -- Thread-Index: AchjRrEXp2t+N5l4QUO/MqsR+Ixv+AAqcVsg } 22, 23 -- In-Reply-To: {200801301347.m0UDlhHR001876-at-ns.microscopy.com} } 22, 23 -- Content-Transfer-Encoding: 8bit } 22, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id m0VAmQvl026363 } ==============================End of - } Headers============================== }
==============================Original Headers============================== 6, 23 -- From Elliott-at-arizona.edu Thu Jan 31 10:06:28 2008 6, 23 -- Received: from smtpgate.email.arizona.edu (gandalf.email.Arizona.EDU [128.196.133.169]) 6, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0VG6Rrq012449 6, 23 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 31 Jan 2008 10:06:27 -0600 6, 23 -- Received: from gandalfs_amavis (amavis4.email.arizona.edu [10.0.0.207]) 6, 23 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 10A6F3DF99B 6, 23 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 31 Jan 2008 09:06:27 -0700 (MST) 6, 23 -- Received: from [150.135.145.126] (unknown [150.135.145.126]) 6, 23 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 4F2AF3DF994 6, 23 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 31 Jan 2008 09:06:23 -0700 (MST) 6, 23 -- Mime-Version: 1.0 (Apple Message framework v753) 6, 23 -- In-Reply-To: {200801311052.m0VAqILG031107-at-ns.microscopy.com} 6, 23 -- References: {200801311052.m0VAqILG031107-at-ns.microscopy.com} 6, 23 -- Content-Type: text/plain; charset=ISO-8859-1; delsp=yes; format=flowed 6, 23 -- Message-Id: {05B35F5A-AA6E-442A-9337-89C5833EE59B-at-arizona.edu} 6, 23 -- From: David Elliott {Elliott-at-arizona.edu} 6, 23 -- Subject: Re: [Microscopy] RE: TEM - negative scanners 6, 23 -- Date: Thu, 31 Jan 2008 09:06:22 -0700 6, 23 -- To: Microscopy ListServer {Microscopy-at-MSA.Microscopy.Com} 6, 23 -- X-Mailer: Apple Mail (2.753) 6, 23 -- X-Virus-Scanned: amavisd-new at email.arizona.edu 6, 23 -- Content-Transfer-Encoding: 8bit 6, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m0VG6Rrq012449 ==============================End of - Headers==============================
I agree with Jessica that you should use cryomicrotomy and collect dry sections. This is a challenging technique but with practice you will find it to be valuable. Microtome vendors and other training facilities offer workshops on the cryomicrotomy.
In our lab we use a 'plastic chuck' for sectioning films at ambient and low temperature. The chuck consists of a piece of plastic with approximate dimensions of 4 mm x 7 mm x 3 mm (width x length x thickness). These dimensions should be set to your personal preference and to fit your sample and microtome chuck. Use a stiff plastic such as high density polyethylene or polypropylene that will hold the sample tight but is not rigid or inflexible. Using a sharp razor blade, bisect the 3 mm thickness, cutting through nearly the full length of the plastic piece and leaving a couple of millimeters of at the other end of the piece to hold the arms of the chuck together. The end results is a Y-shaped piece of plastic (see diagram below). To use the chuck, slide the film between the arms of the plastic chuck leaving less than 1 mm exposed; the more film is exposed, the more likely it is to move side-to-side during microtomy. Listers may contact me off-line for a drawing of the chuck.
I recommend that you do not embed films in epoxy if at all possible. The embedding medium, whatever it might be, only contributes to the difficulty of cutting. The problem is that the film has several layers, each with different cutting properties at low temperature; the problem is often even worse at ambient temperature. Adding the embedding medium contributes another medium that most probably has very different cutting properties than any material in the film.
Disclaimer: The comments and opinions given above are those of the author alone and do not represent any position of ExxonMobil Chemical Company.
Regards,
Gary M. Brown Microscopy Specialist ExxonMobil Chemical Company Baytown Technology & Engineering - West 5200 Bayway Drive Baytown, Texas 77520-2101 phone: 281 834 2387 fax: 281 834 2395 e-mail: Gary.M.Brown-at-ExxonMobil.com
"Our business in life is not to succeed, but to continue to fail in good spirits." Robert Louis Stevenson
cervantes-at-bend res.com To gary.m.brown-at-exxonmobil.com 01/30/08 07:40 cc PM Subject [Microscopy] RE: viaWWW: 2 questions Please respond concerning microtomy of thin films to cervantes-at-bend res.com
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Sandra -
I also work with water soluble materials, and so often have to cut dry. I have found that a diamond knife is preferable to glass (less "sticky" so sections are easier to get off the knife surface), but that may be something you want to experiment with. To transfer sections from the knife to the grid, I use an eyelash probe (I buy mine from Ted Pella, but people make them as well). It takes a little practice, but that is what works best for me. I also use the probe to gently "flatten" the sections onto the grid surface (I use 300 mesh Copper grids with Formvar support film).
Diatome used to make a Cryo P diamond knife (don't know if they still do) with a special platform that makes it easier to transfer sections. This has been my favorite knife for dry-sectioning.
Sorry can't help with your second question, but hope this was useful.
Jessica
____________________ Jessica Cervantes Bend Research Inc 64550 Research Rd Bend, OR 97701 www.bendres.com
-----Original Message----- X-from: sandra.gardner-at-xerox.com [mailto:sandra.gardner-at-xerox.com] Sent: Wednesday, January 30, 2008 2:49 PM To: Cervantes, Jessica
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Title-Subject: [Filtered] 2 questions concerning microtomy of thin films
Question: I have a multi layer film in which one of the layers is water soluble. We want to cross section the sample for TEM to view the various layers, each of which are less than 500nm thick. The total thickness of the plastic substrate plus multilayer film is aprox. 500microns (the substate is aprox 300microns of this). I've embedded the film in a epoxy resin for sectioning. I don't know how to go about capturing thin sections ( {100nm) if I can't put water in the boat. I could cut them dry, but again, I'm not sure how to transfer them to the grid. I am using a Diatome diamond knife. Any suggestions would be greatly appreciated.
Another question I have regards the use of tape as a support media for cryo-sectioning. Many of our samples are films which I typically embed in epoxy resin. We now have cryo-sectioning capabilities and i would like to simply sandwich my film between tape and cryo cut thin films for TEM analyses. Is there any tape which has good adhesion properties at -120C and stand up well for TEM (glue does not interfere with sample)
As I have suggested in the past, a digital camera can be used to copy TEM negatives (or any larger format film). I put my negatives, emulsion side up, on a light box, mask off the unwanted light with heavy black paper or cardboard, and photograph the negative with an older (4 years or so) Canon G3 camera mounted on a copy stand to insure stability. Most better digital cameras will focus close enough to fill the frame with the negative (3.25 x 4 inches). I download the images to Photoshop and invert to a positive. This is much faster than using a flatbed scanner. The quality is fine for most applications. If you must have higher quality for publication or making mural size prints, take the negatives to a shop that has a drum scanner.
Geoff vhacopian-at-wellesley.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I wish to purchase a flatbed scanner for TEM negatives. I have received } recommendations for the Canon 9950F and the Epson V750-M. The former may } no longer be available, as it is not listed on the Canon web site--only } the 8800F is. Any suggestions would be welcome and highly appreciated. } Vachik Hacopian } } } } -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff-at-umdnj.edu **********************************************
==============================Original Headers============================== 5, 28 -- From mcauliff-at-umdnj.edu Thu Jan 31 10:15:48 2008 5, 28 -- Received: from zix04.umdnj.edu (zix04.UMDNJ.EDU [130.219.34.127]) 5, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0VGFlbo027927 5, 28 -- for {microscopy-at-microscopy.com} ; Thu, 31 Jan 2008 10:15:48 -0600 5, 28 -- Received: from zix04.umdnj.edu (ZixVPM [127.0.0.1]) 5, 28 -- by Outbound.umdnj.edu (Proprietary) with ESMTP id 41CDB4BF14 5, 28 -- for {microscopy-at-microscopy.com} ; Thu, 31 Jan 2008 11:15:46 -0500 (EST) 5, 28 -- Received: from umdnj.edu (imail2.UMDNJ.EDU [130.219.34.139]) 5, 28 -- by zix04.umdnj.edu (Proprietary) with ESMTP id 778864BE91 5, 28 -- for {microscopy-at-microscopy.com} ; Thu, 31 Jan 2008 11:15:45 -0500 (EST) 5, 28 -- Received: from ([10.32.15.171]) 5, 28 -- by imail2.umdnj.edu with ESMTP id KP-BRCRF.140113879; 5, 28 -- Thu, 31 Jan 2008 11:15:20 -0500 5, 28 -- MIME-version: 1.0 5, 28 -- Content-transfer-encoding: 7BIT 5, 28 -- Content-type: text/plain; charset=ISO-8859-1; format=flowed 5, 28 -- Received: from [127.0.0.1] ([10.32.15.102]) 5, 28 -- by umduwc02.umdnj.edu (Sun Java(tm) System Messaging Server 6.3-5.02 (built 5, 28 -- Oct 12 2007; 32bit)) with ESMTP id {0JVI00M3DNTJ8530-at-umduwc02.umdnj.edu} for 5, 28 -- microscopy-at-microscopy.com; Thu, 31 Jan 2008 11:15:20 -0500 (EST) 5, 28 -- Message-id: {47A1F430.8030401-at-umdnj.edu} 5, 28 -- Date: Thu, 31 Jan 2008 11:15:44 -0500 5, 28 -- From: Geoff McAuliffe {mcauliff-at-umdnj.edu} 5, 28 -- User-Agent: Thunderbird 2.0.0.9 (Windows/20071031) 5, 28 -- To: vhacopian-at-wellesley.edu, microscopy-at-microscopy.com 5, 28 -- Subject: Re: [Microscopy] TEM - negative scanners 5, 28 -- References: {200801301338.m0UDc1UX023749-at-ns.microscopy.com} 5, 28 -- In-reply-to: {200801301338.m0UDc1UX023749-at-ns.microscopy.com} ==============================End of - Headers==============================
I doubt if microwave exposure (cooking) will soften embalmed tissue. You might try soaking the organ in water (tap water) for a week, changing the water every day. There is good evidence that formalin-fixed tissue can be "unfixed", at least to some extent, by prolonged washing in water. Of course, it will depend on the composition of the embalming fluid, the length of the exposure, etc.
Geoff
tbargar-at-unmc.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } I've got a surgeon who is working with human livers that are very hard from } the embalming process and he had heard that microwaving tissue may soften } up the tissue and make it easier to cut. I'm going to let him use my } Biowave and try different wattages. I have never heard of this effect } before. Has anybody out there heard of or actually know if hardened fixed } tissue can be softened by microwaves. Thanks, all help is appreciated. } } Tom Bargar } University of Nebraska Medical Center } Core Electron Microscopy Research Facility } 986395 Nebraska Medical Center } Omaha, NE 68198-6395 } 402-559-7347 } tbargar-at-unmc.edu } } } ==============================Original Headers============================== } 4, 20 -- From tbargar-at-unmc.edu Tue Jan 29 09:29:00 2008 } 4, 20 -- Received: from zixvpm01.unmc.edu (zixvpm01.unmc.edu [192.198.54.126]) } 4, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0TFT028004622 } 4, 20 -- for {Microscopy-at-MSA.Microscopy.com} ; Tue, 29 Jan 2008 09:29:00 -0600 } 4, 20 -- Received: from zixvpm01.unmc.edu (ZixVPM [127.0.0.1]) } 4, 20 -- by Outbound.unmc.edu (Proprietary) with ESMTP id 2FE894C084 } 4, 20 -- for {Microscopy-at-MSA.Microscopy.com} ; Tue, 29 Jan 2008 09:29:00 -0600 (CST) } 4, 20 -- Received: from unmcnotes01.unmc.edu (host-137-197-103-35.unmc.edu [137.197.103.35]) } 4, 20 -- by zixvpm01.unmc.edu (Proprietary) with ESMTP id C51AF4C06E } 4, 20 -- for {Microscopy-at-MSA.Microscopy.com} ; Tue, 29 Jan 2008 09:28:58 -0600 (CST) } 4, 20 -- Subject: Can hardened fixed tissue be "softened" by Microwave treatment? } 4, 20 -- To: Microscopy-at-MSA.Microscopy.com } 4, 20 -- X-Mailer: Lotus Notes Release 7.0.1 January 17, 2006 } 4, 20 -- Message-ID: {OF0B63F2D6.945FCCD8-ON862573DF.005434C9-862573DF.00550C8B-at-unmc.edu} } 4, 20 -- From: Tom W Bargar {tbargar-at-unmc.edu} } 4, 20 -- Date: Tue, 29 Jan 2008 09:28:57 -0600 } 4, 20 -- X-MIMETrack: Serialize by Router on UNMCNOTES01.UNMC.EDU/Servers/UNEBR at 01/29/2008 09:28:58 } 4, 20 -- AM } 4, 20 -- MIME-Version: 1.0 } 4, 20 -- Content-type: text/plain; charset=US-ASCII } ==============================End of - Headers============================== } } }
-- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff-at-umdnj.edu **********************************************
==============================Original Headers============================== 9, 29 -- From mcauliff-at-umdnj.edu Thu Jan 31 10:21:58 2008 9, 29 -- Received: from zix04.umdnj.edu (zix04.UMDNJ.EDU [130.219.34.127]) 9, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0VGLuWl014928 9, 29 -- for {microscopy-at-microscopy.com} ; Thu, 31 Jan 2008 10:21:57 -0600 9, 29 -- Received: from zix04.umdnj.edu (ZixVPM [127.0.0.1]) 9, 29 -- by Outbound.umdnj.edu (Proprietary) with ESMTP id 0E6224BF07 9, 29 -- for {microscopy-at-microscopy.com} ; Thu, 31 Jan 2008 11:21:56 -0500 (EST) 9, 29 -- Received: from umdnj.edu (imail2.UMDNJ.EDU [130.219.34.139]) 9, 29 -- by zix04.umdnj.edu (Proprietary) with ESMTP id 242A54BE8B 9, 29 -- for {microscopy-at-microscopy.com} ; Thu, 31 Jan 2008 11:21:55 -0500 (EST) 9, 29 -- Received: from ([10.32.15.171]) 9, 29 -- by imail2.umdnj.edu with ESMTP id KP-BRCRF.140114859; 9, 29 -- Thu, 31 Jan 2008 11:21:30 -0500 9, 29 -- MIME-version: 1.0 9, 29 -- Content-transfer-encoding: 7BIT 9, 29 -- Content-type: text/plain; charset=ISO-8859-1; format=flowed 9, 29 -- Received: from [127.0.0.1] ([10.32.15.102]) 9, 29 -- by umduwc02.umdnj.edu (Sun Java(tm) System Messaging Server 6.3-5.02 (built 9, 29 -- Oct 12 2007; 32bit)) with ESMTP id {0JVI00MMNO3U8530-at-umduwc02.umdnj.edu} for 9, 29 -- microscopy-at-microscopy.com; Thu, 31 Jan 2008 11:21:30 -0500 (EST) 9, 29 -- Message-id: {47A1F5A2.4070709-at-umdnj.edu} 9, 29 -- Date: Thu, 31 Jan 2008 11:21:54 -0500 9, 29 -- From: Geoff McAuliffe {mcauliff-at-umdnj.edu} 9, 29 -- User-Agent: Thunderbird 2.0.0.9 (Windows/20071031) 9, 29 -- To: tbargar-at-unmc.edu, microscopy-at-microscopy.com 9, 29 -- Subject: Re: [Microscopy] Can hardened fixed tissue be "softened" by Microwave 9, 29 -- treatment? 9, 29 -- References: {200801291530.m0TFUBG4007543-at-ns.microscopy.com} 9, 29 -- In-reply-to: {200801291530.m0TFUBG4007543-at-ns.microscopy.com} ==============================End of - Headers==============================
My IGP on my CM series TEM lasted 11-13 years. 4 years is quite short., IMO.
If you think you have carbon or hydrocarbon issues, have your serviceman check the long column tubing liner just under the anode cap for a black coating of residue that builds up on the liner's inner wall. There should be a tool in the factory tool kit to remove this tubing liner. Look at the amount of the deposit and the color. Ask the serviceman if that amount of deposit seems normal or excessive. I would have a supply of 6-8 inch long wooden "Q-tip swabs" and Pol polish handy for him.
HTH,
Paul
At 09:01 AM 1/30/08 -0600, you wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 6, 16 -- From beaurega-at-westol.com Thu Jan 31 11:24:28 2008 6, 16 -- Received: from mail.winbeam.com (mail.winbeam.com [64.84.96.10]) 6, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m0VHOSca002606 6, 16 -- for {microscopy-at-microscopy.com} ; Thu, 31 Jan 2008 11:24:28 -0600 6, 16 -- Received: (qmail 16520 invoked by uid 89); 31 Jan 2008 17:24:26 -0000 6, 16 -- Received: from pitts-69-72-117-2.dynamic-dialup.coretel.net (HELO millenium) (69.72.117.2) 6, 16 -- by mail.winbeam.com with SMTP; 31 Jan 2008 17:24:26 -0000 6, 16 -- Message-Id: {3.0.6.32.20080131122424.007b6420-at-pop3.norton.antivirus} 6, 16 -- X-Sender: beaurega/mail.westol.com-at-pop3.norton.antivirus (Unverified) 6, 16 -- X-Mailer: QUALCOMM Windows Eudora Light Version 3.0.6 (32) 6, 16 -- Date: Thu, 31 Jan 2008 12:24:24 -0500 6, 16 -- To: nizets2-at-yahoo.com, microscopy-at-microscopy.com 6, 16 -- From: Beaurega {beaurega-at-westol.com} 6, 16 -- Subject: Re: [Microscopy] ion pump longevity 6, 16 -- Mime-Version: 1.0 6, 16 -- Content-Type: text/plain; charset="us-ascii" ==============================End of - Headers==============================
A modern flatbed film scanner (e.g. the Epson V750) can scan a negative into PhotoShop within a few minutes at 1,200 dpi - and there's no photo download from camera to PC involved afterwards. At 6,400 dpi things do take a bit longer, but that's well beyond the resolution of just about any camera prime lens and indeed most colour slide film for that matter. If you compare a camera macro image of a negative/slide to an Epson V750 film scan, it's no contest - the Epson wins hands down.
TEM film has a higher theoretical resolution than 6,400 dpi but the resulting TIF image file size from a slow 6,400 dpi V750 scan would be highly prone to corruption if archived digitally on a PC anyway. You can easily use 3,200 dpi to 'enlarge' selected areas of the negative though. Plus we don't need Digital ICE scratch removal, which is the main process that markedly slows down film scan speeds [but it can introduce artifacts and affect detail, and anyway it isn't available for B&W film].
Besides, any process that passes an image back through another set of optics will always further degrade that image. You should use a 8-10x inspection magnifier and a light box to compare the scanned image with the original film - the difference in resolution is small but clearly visible. Whatever scanner is used, some detail is lost (just as detail was lost when the original view was captured). Plus you really need to get the film/specimen height and flatness right on a flatbed scanner, normally this means using the supplied film holder (if the films at the wrong height from the platen the scan image can be out of focus).
You do always get an apparent large reduction in shadow detail with a pro-sumer V750 type flat bed scanner with colour slide film (shadows look far too black), but the detail is actually still there and you can bring it right back to the correct level of brightness using Photoshop's 'shadow/highlight' command. This quickly restores the shadow detail to the scanned image. You can use PhotoShop's 'unsharp mask' [not much] to add back a bit of sharpness at 100% mag as well. Always look at the original film using a light box and 8-10x magnifier to ensure you get this post-scan processing just right if that’s important to you.
The Epson 750 Pro produces image scans of comparable (if not indistinguishable) quality to those of an £10k Hasselblad Imacon Flextight 848 semi-drum scanner - granted at far lower scan speeds. Besides 100 TEM film scans using a Hasselblad in your Reprographics department would cost you more than an Epson V750 pro anyway.
Keith
-------------------------------------------------------------------------- Dr keith J. Morris Molecular Cytogenetics and Microscopy Core Laboratory 00/069 and 00/070 The Wellcome Trust Centre for Human Genetics Roosevelt Drive Oxford OX3 7BN United Kingdom Telephone: +44 (0)1865 287568 Email: kjmorris-at-well.ox.ac.uk Web-pages: http://www.well.ox.ac.uk/cytogenetics/
-----Original Message----- X-from: mcauliff-at-umdnj.edu [mailto:mcauliff-at-umdnj.edu] Sent: 31 January 2008 16:23 To: kjmorris-at-well.ox.ac.uk
Greetings all:
As I have suggested in the past, a digital camera can be used to copy TEM negatives (or any larger format film). I put my negatives, emulsion side up, on a light box, mask off the unwanted light with heavy black paper or cardboard, and photograph the negative with an older (4 years or so) Canon G3 camera mounted on a copy stand to insure stability. Most better digital cameras will focus close enough to fill the frame with the negative (3.25 x 4 inches). I download the images to Photoshop and invert to a positive. This is much faster than using a flatbed scanner. The quality is fine for most applications. If you must have higher quality for publication or making mural size prints, take the negatives to a shop that has a drum scanner.
Geoff vhacopian-at-wellesley.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I wish to purchase a flatbed scanner for TEM negatives. I have received } recommendations for the Canon 9950F and the Epson V750-M. The former may } no longer be available, as it is not listed on the Canon web site--only } the 8800F is. Any suggestions would be welcome and highly appreciated. } Vachik Hacopian } } } } -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff-at-umdnj.edu **********************************************
==============================Original Headers============================== 5, 28 -- From mcauliff-at-umdnj.edu Thu Jan 31 10:15:48 2008 5, 28 -- Received: from zix04.umdnj.edu (zix04.UMDNJ.EDU [130.219.34.127]) 5, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0VGFlbo027927 5, 28 -- for {microscopy-at-microscopy.com} ; Thu, 31 Jan 2008 10:15:48 -0600 5, 28 -- Received: from zix04.umdnj.edu (ZixVPM [127.0.0.1]) 5, 28 -- by Outbound.umdnj.edu (Proprietary) with ESMTP id 41CDB4BF14 5, 28 -- for {microscopy-at-microscopy.com} ; Thu, 31 Jan 2008 11:15:46 -0500 (EST) 5, 28 -- Received: from umdnj.edu (imail2.UMDNJ.EDU [130.219.34.139]) 5, 28 -- by zix04.umdnj.edu (Proprietary) with ESMTP id 778864BE91 5, 28 -- for {microscopy-at-microscopy.com} ; Thu, 31 Jan 2008 11:15:45 -0500 (EST) 5, 28 -- Received: from ([10.32.15.171]) 5, 28 -- by imail2.umdnj.edu with ESMTP id KP-BRCRF.140113879; 5, 28 -- Thu, 31 Jan 2008 11:15:20 -0500 5, 28 -- MIME-version: 1.0 5, 28 -- Content-transfer-encoding: 7BIT 5, 28 -- Content-type: text/plain; charset=ISO-8859-1; format=flowed 5, 28 -- Received: from [127.0.0.1] ([10.32.15.102]) 5, 28 -- by umduwc02.umdnj.edu (Sun Java(tm) System Messaging Server 6.3-5.02 (built 5, 28 -- Oct 12 2007; 32bit)) with ESMTP id {0JVI00M3DNTJ8530-at-umduwc02.umdnj.edu} for 5, 28 -- microscopy-at-microscopy.com; Thu, 31 Jan 2008 11:15:20 -0500 (EST) 5, 28 -- Message-id: {47A1F430.8030401-at-umdnj.edu} 5, 28 -- Date: Thu, 31 Jan 2008 11:15:44 -0500 5, 28 -- From: Geoff McAuliffe {mcauliff-at-umdnj.edu} 5, 28 -- User-Agent: Thunderbird 2.0.0.9 (Windows/20071031) 5, 28 -- To: vhacopian-at-wellesley.edu, microscopy-at-microscopy.com 5, 28 -- Subject: Re: [Microscopy] TEM - negative scanners 5, 28 -- References: {200801301338.m0UDc1UX023749-at-ns.microscopy.com} 5, 28 -- In-reply-to: {200801301338.m0UDc1UX023749-at-ns.microscopy.com} ==============================End of - Headers==============================
==============================Original Headers============================== 21, 23 -- From kjmorris-at-well.ox.ac.uk Thu Jan 31 11:33:56 2008 21, 23 -- Received: from morse.well.ox.ac.uk (morse.well.ox.ac.uk [129.67.44.2]) 21, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0VHXuHx016349 21, 23 -- for {Microscopy-at-Microscopy.Com} ; Thu, 31 Jan 2008 11:33:56 -0600 21, 23 -- Received: from dhcp079.well.ox.ac.uk ([129.67.44.178] helo=CytoWhizz) 21, 23 -- by morse.well.ox.ac.uk with esmtp (Exim 4.52) 21, 23 -- id 1JKdIR-0006Do-Qk 21, 23 -- for Microscopy-at-Microscopy.Com; Thu, 31 Jan 2008 17:33:55 +0000 21, 23 -- From: "Keith Morris" {kjmorris-at-well.ox.ac.uk} 21, 23 -- To: {Microscopy-at-Microscopy.Com} 21, 23 -- References: {200801311623.m0VGN5t8018582-at-ns.microscopy.com} 21, 23 -- Subject: RE: [Microscopy] Re: TEM - negative scanners 21, 23 -- Date: Thu, 31 Jan 2008 17:34:08 -0000 21, 23 -- Message-ID: {000901c8642f$7c139dc0$b22c4381-at-CytoWhizz} 21, 23 -- MIME-Version: 1.0 21, 23 -- Content-Type: text/plain; 21, 23 -- charset="iso-8859-1" 21, 23 -- X-Mailer: Microsoft Office Outlook 11 21, 23 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 21, 23 -- Thread-Index: AchkJY/ZmC9QjEHURfuwINJhalAmEgAATiMg 21, 23 -- In-Reply-To: {200801311623.m0VGN5t8018582-at-ns.microscopy.com} 21, 23 -- Content-Transfer-Encoding: 8bit 21, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m0VHXuHx016349 ==============================End of - Headers==============================
I suspect that the Canon and Epson scanners will be more readily available for negatives but it is still worth checking the specification and prices of the Microtek i800 (semi pro) and i900 (pro). They both have pretty good resolution and an excellect Dmax as well as using a separate glassless drawer for negatives up to about 12 x 10 inches.
eg i800 review: "What's affordable about $400 list? How about a Dmax of 4.0, 48-bit color and 9600x4800 dpi optical resolution on a legal-sized scanning bed with your choice of High-Speed USB 2.0 or FireWire ports?" from http://www.imaging-resource.com/SCAN/MI8/MI8.HTM
The i900 is about twice the price but has an improved Dmax of 4.2.
I personally use the Microtek Scanmaker 8700 which still serves me well but doesn't have the resolution or high Dmax of the two above.
Malcolm
Malcolm Haswell e.m. unit School of Health, Natural and Social Sciences Fleming Building University of Sunderland Tyne & Wear SR1 3SD UK e-mail: malcolm.haswell-at-sunderland.ac.uk
----- Original Message ----- X-from: vhacopian-at-wellesley.edu
In response to Tom Bargar's question,
I am not aware that microwaves can soften embalmed human liver tissues. On the other hand, plant biologists often soak plant tissues in glycerol to soften them for sectioning.
Check out the following article:
John C. Guenther and Frances Trail. 2005. The development and differentiation of Gibberella zeae (anamorph: Fusarium graminearum) during colonization of wheat. Mycologia, 97(1), 2005, pp. 229-237.
In this study, the authors did the following:
After a 24 h fixation, samples were placed in a solution of glycerol and ethanol (1:1) for an additional 24 h to soften tissues for sectioning.
They then further dehydrated the specimens in alcohol, embedded (in paraffin, in this case) and sectioned as usual. You might give it a try. Certainly, use the web to search for the use of glycerine as a softener since I know it is used successfully.
John J. Bozzola, Ph.D., Director Integrated Microscopy & Graphics Expertise (IMAGE) Southern Illinois University 750 Communications Drive - MC 4402 Carbondale, IL 62901 Telephone: 618-453-3730
All: is there anyone in the north Texas area that has an SEM that will take a 300mm (12-inch) wafer? I need to image the surface of a fully processed, bumped wafer without coating it or breaking it.
-- ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Becky Holdford (r-holdford-at-ti.com) 972-995-2360 972-648-8743 (pager) SC Packaging FA Development Texas Instruments, Inc. Dallas, TX ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
==============================Original Headers============================== 3, 21 -- From r-holdford-at-ti.com Thu Jan 31 16:15:53 2008 3, 21 -- Received: from soda.ext.ti.com (soda.ext.ti.com [198.47.26.145]) 3, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m0VMFrei018228 3, 21 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 31 Jan 2008 16:15:53 -0600 3, 21 -- Received: from dlep32.itg.ti.com ([157.170.170.70]) 3, 21 -- by soda.ext.ti.com (8.13.7/8.13.7) with ESMTP id m0VMFllG014552 3, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 3, 21 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 31 Jan 2008 16:15:52 -0600 3, 21 -- Received: from [156.117.194.120] (localhost [127.0.0.1]) 3, 21 -- by dlep32.itg.ti.com (8.13.7/8.13.7) with ESMTP id m0VMFlia025841 3, 21 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 31 Jan 2008 16:15:47 -0600 (CST) 3, 21 -- Message-ID: {47A24892.2020405-at-ti.com} 3, 21 -- Date: Thu, 31 Jan 2008 16:15:46 -0600 3, 21 -- From: Becky Holdford {r-holdford-at-ti.com} 3, 21 -- Organization: SC Packaging Development -- FA Development 3, 21 -- User-Agent: Thunderbird 2.0.0.9 (Windows/20071031) 3, 21 -- MIME-Version: 1.0 3, 21 -- To: MSA ListServer {Microscopy-at-MSA.Microscopy.Com} 3, 21 -- Subject: 300mm wafer-capable SEM in North Texas? 3, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 3, 21 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
The Microbeam Analysis Society is sponsoring a Technical Conference on EBSD May 20-22 at the University of Wisconsin-Madison. The 3 day workshop will start with a full day tutorial session for newcomers to the technique. Student participation is being encouraged with 10 $500 scholarships. For more information and online registration materials, go to www. microbeamanalysis.org. Space is limited so early registration is strongly recommended.
John Fournelle johnf-at-geology.wisc.edu
==============================Original Headers============================== 2, 24 -- From johnf-at-geology.wisc.edu Thu Jan 31 18:09:44 2008 2, 24 -- Received: from mail.geology.wisc.edu (mail.geology.wisc.edu [144.92.206.10]) 2, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1109iK2003552 2, 24 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 31 Jan 2008 18:09:44 -0600 2, 24 -- Received: from localhost (mail.geology.wisc.edu [127.0.0.1]) 2, 24 -- by localhost (Postfix) with ESMTP id 095DD5C812D 2, 24 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 31 Jan 2008 18:09:44 -0600 (CST) 2, 24 -- X-Virus-Scanned: amavisd-new at geology.wisc.edu 2, 24 -- Received: from mail.geology.wisc.edu ([127.0.0.1]) 2, 24 -- by localhost (mail.geology.wisc.edu [127.0.0.1]) (amavisd-new, port 10024) 2, 24 -- with ESMTP id s1dDmOaTrh0F for {Microscopy-at-MSA.Microscopy.Com} ; 2, 24 -- Thu, 31 Jan 2008 18:09:37 -0600 (CST) 2, 24 -- Received: from [144.92.206.57] (beamer.geology.wisc.edu [144.92.206.57]) 2, 24 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 2, 24 -- (No client certificate requested) 2, 24 -- by mail.geology.wisc.edu (Postfix) with ESMTP id 6DBC15C8117 2, 24 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 31 Jan 2008 18:09:37 -0600 (CST) 2, 24 -- Mime-Version: 1.0 2, 24 -- Message-Id: {p0623091ec3c812cab2e5-at-[144.92.206.57]} 2, 24 -- Date: Thu, 31 Jan 2008 18:09:35 -0600 2, 24 -- To: Microscopy-at-MSA.Microscopy.Com 2, 24 -- From: John Fournelle {johnf-at-geology.wisc.edu} 2, 24 -- Subject: EBSD workshop May 20-22 2, 24 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mikroskop-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: mikroskop-at-gmail.com Name: Jack Hietpas
Organization: Syracuse University
Title-Subject: [Filtered] magnification in CL images
Question: Hello: We have a JEOL 8600 Superprobe with what has been described as the "Poor man's" cathodoluminescence (CL) detector, which is simply a PMT inserted in the optical path of the probe's integrated light microscope. I have a very basic question concerning the system: where does the magnification of CL image come from? In addition why is the field of view the exact same as that in BSE or SEI? I find it hard to believe (perhaps incorrectly) that the magnification comes from the light microscope: the magnification is continuous as opposed to the fixed mag (focal length) of the light microscope and the mag range of the CL images is beyond the useful mag of the microscope. What am I missing?
I don't know your exact setup, but my guess is that the signal from the PMT is synchronized with the beam scanning on the sample. For each position on the sample (pixel) the magnitude of the PMT is recorded as the intensity on the image. Just like a SE or BSE images.
Hendrix
On Jan 31, 2008 7:40 PM, {mikroskop-at-gmail.com} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both mikroskop-at-gmail.com as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: mikroskop-at-gmail.com } Name: Jack Hietpas } } Organization: Syracuse University } } Title-Subject: [Filtered] magnification in CL images } } Question: Hello: We have a JEOL 8600 Superprobe with what has been described as the "Poor man's" cathodoluminescence (CL) detector, which is simply a PMT inserted in the optical path of the probe's integrated light microscope. I have a very basic question concerning the system: where does the magnification of CL image come from? In addition why is the field of view the exact same as that in BSE or SEI? I find it hard to believe (perhaps incorrectly) that the magnification comes from the light microscope: the magnification is continuous as opposed to the fixed mag (focal length) of the light microscope and the mag range of the CL images is beyond the useful mag of the microscope. What am I missing? } } -jh } } } Login Host: 69.205.65.34 } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 8, 11 -- From zaluzec-at-microscopy.com Thu Jan 31 18:34:01 2008 } 8, 11 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m110XxSQ016698 } 8, 11 -- for {microscopy-at-microscopy.com} ; Thu, 31 Jan 2008 18:34:00 -0600 } 8, 11 -- Mime-Version: 1.0 } 8, 11 -- Message-Id: {p06240803c3c81966eb8e-at-[206.69.208.22]} } 8, 11 -- Date: Thu, 31 Jan 2008 18:33:58 -0600 } 8, 11 -- To: microscopy-at-microscopy.com } 8, 11 -- From: mikroskop-at-gmail.com (by way of MicroscopyListserver) } 8, 11 -- Subject: viaWWW: magnification in CL images } 8, 11 -- Content-Type: text/plain; charset="us-ascii" } ==============================End of - Headers============================== }
==============================Original Headers============================== 4, 30 -- From drix00-at-gmail.com Thu Jan 31 19:11:44 2008 4, 30 -- Received: from an-out-0708.google.com (an-out-0708.google.com [209.85.132.249]) 4, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m111BhZc030147 4, 30 -- for {microscopy-at-microscopy.com} ; Thu, 31 Jan 2008 19:11:44 -0600 4, 30 -- Received: by an-out-0708.google.com with SMTP id b33so224852ana.21 4, 30 -- for {microscopy-at-microscopy.com} ; Thu, 31 Jan 2008 17:11:43 -0800 (PST) 4, 30 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 30 -- d=gmail.com; s=gamma; 4, 30 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:cc:in-reply-to:mime-version:content-type:content-transfer-encoding:content-disposition:references; 4, 30 -- bh=QmtmXaAwdd+cpAgKgBkc/W1R96IpgB3pQeYIOiuK0SM=; 4, 30 -- b=wjMK8fSGAuExBtV3TWnZiE+4QPtelxnQgJcLrbaTOHo14XvxXah3z52eMxBlVNMqYy+nb5xokNxQjsZP3lovq8Keyt+8QXnID9Nn4SkIizOuteiPFpg99h1gJOYPlji7iO8Kfg7J3B0tW7GgehSPjtj2hy+hOEbU+Br4A9Zf2fk= 4, 30 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 4, 30 -- d=gmail.com; s=gamma; 4, 30 -- h=message-id:date:from:to:subject:cc:in-reply-to:mime-version:content-type:content-transfer-encoding:content-disposition:references; 4, 30 -- b=suK0R7qfXBlDj+kd5Wob9vjDoeA3lk70JL/FUAKSQEPupeM9JcaRXLhGAhIHwiX8BjR6K9KxfF5rJLCNNqqiqTyrBjCB2gWgbaEGvF2dKhCxbbzm4gAs3QRY6tf7oEozYBgnQ93KuarOfo+c6cgw5DB+EjN+7KGyPz2+H55Us5Q= 4, 30 -- Received: by 10.101.70.5 with SMTP id x5mr5961529ank.59.1201828303554; 4, 30 -- Thu, 31 Jan 2008 17:11:43 -0800 (PST) 4, 30 -- Received: by 10.100.91.18 with HTTP; Thu, 31 Jan 2008 17:11:43 -0800 (PST) 4, 30 -- Message-ID: {a779eeae0801311711y4f33dfa4hbf10abfc046f80e8-at-mail.gmail.com} 4, 30 -- Date: Thu, 31 Jan 2008 20:11:43 -0500 4, 30 -- From: drix {drix00-at-gmail.com} 4, 30 -- To: mikroskop-at-gmail.com 4, 30 -- Subject: Re: [Microscopy] viaWWW: magnification in CL images 4, 30 -- Cc: microscopy-at-microscopy.com 4, 30 -- In-Reply-To: {200802010040.m110eUxR025164-at-ns.microscopy.com} 4, 30 -- MIME-Version: 1.0 4, 30 -- Content-Type: text/plain; charset=ISO-8859-1 4, 30 -- Content-Transfer-Encoding: 7bit 4, 30 -- Content-Disposition: inline 4, 30 -- References: {200802010040.m110eUxR025164-at-ns.microscopy.com} ==============================End of - Headers==============================
I have someone who wants to look at "changes" at the EM level caused by experimental drugs, in guinea pig eyes. Hopefully he'll come up with a more specific aim, but in the meantime does anyone have experience fixing guinea pigs? I'll get him to perfuse the animals, but would like to choose the best fixative. Any suggestions?
Thanks
Diana
Diana van Driel
Discipline of Ophthalmology Sydney University GPO Box 4337 Sydney, NSW AUSTRALIA 2001
The Microtek i800 scanner optical resolution is quoted at 4,800 dpi to the Epsons V700/V750s 6,400 dpi - so it's probably on a par with the older 4,800 dpi Epsons and Canons - both of which were perfectly fine for TEM film scans at 1,200 dpi anyway (other than Canon's Scangear being too stupid to notice any film was on the platter).
Dmax is less important with B&W film (and this includes TEM) but is essential resolving shadows with colour slides. Most modern scanners will have a high enough DMax for B&W TEM film. The Hasselblad flextight has a superb DMax and shadow detail in a colour slide was resolved very well, but no better than the cheaper Epsons after post-processsing (the colour slide film itself was the limiting factor really). The better resolution of the 8,000 dpi Hasselblad Flextight 848 drum scanner just picked out the detail of the colour film grain better, and surprisingly didn't really get any extra information from B&W TEM film (but I couldn't get to this scanner and tweak it optimally for the TEM film - it was operated by the universities Reprographics staff at £10 a scan.
Keith
-------------------------------------------------------------------------- Dr keith J. Morris Molecular Cytogenetics and Microscopy Core Laboratory 00/069 and 00/070 The Wellcome Trust Centre for Human Genetics Roosevelt Drive Oxford OX3 7BN United Kingdom Telephone: +44 (0)1865 287568 Email: kjmorris-at-well.ox.ac.uk Web-pages: http://www.well.ox.ac.uk/cytogenetics/
-----Original Message----- X-from: malcolm.haswell-at-sunderland.ac.uk [mailto:malcolm.haswell-at-sunderland.ac.uk] Sent: 31 January 2008 17:46 To: kjmorris-at-well.ox.ac.uk
Vachik
I suspect that the Canon and Epson scanners will be more readily available for negatives but it is still worth checking the specification and prices of the Microtek i800 (semi pro) and i900 (pro). They both have pretty good resolution and an excellect Dmax as well as using a separate glassless drawer for negatives up to about 12 x 10 inches.
eg i800 review: "What's affordable about $400 list? How about a Dmax of 4.0, 48-bit color and 9600x4800 dpi optical resolution on a legal-sized scanning bed with your choice of High-Speed USB 2.0 or FireWire ports?" from http://www.imaging-resource.com/SCAN/MI8/MI8.HTM
The i900 is about twice the price but has an improved Dmax of 4.2.
I personally use the Microtek Scanmaker 8700 which still serves me well but doesn't have the resolution or high Dmax of the two above.
Malcolm
Malcolm Haswell e.m. unit School of Health, Natural and Social Sciences Fleming Building University of Sunderland Tyne & Wear SR1 3SD UK e-mail: malcolm.haswell-at-sunderland.ac.uk
----- Original Message ----- X-from: vhacopian-at-wellesley.edu
Diana,
Years ago when we were perfusing mice and hamsters, we used a modified Krebs' Perfusion Buffer to flush the circulatory system, then fixed with a solution of 1% glut, 3% formaldehyde in PBS. Recipe for 100 mL Krebs is as follows (added in the order in which they appear):
0.17 g dextrose 0.02 g NaNO2 0.028 g heparin 2.0 mL 1M HEPES 1.0 g BSA 0.16 mL CaCl2 solution (0.147 g/mL) pH 7.4
It takes about 40 mL to perfuse a mouse, so scale up accordingly.
Daryl Meyer
----- Original Message ----- X-from: dianavd-at-eye.usyd.edu.au
There are three little tricks for extending the life of ion pumps that have not been mentioned yet that I thought I could contribute based on years of experience with JEOL and Topcon TEM's:
1) The hammer. Yes, just smack the bajeezus out of the ion pump body (not the magnet) with a hammer. The shock knocks most of the whiskers and flakes loose. Do it with the emitter cold, because it will shock the whole TEM. Put emphasis on a large number of mild whacks in every direction of the pump housing. Keep the pump HV on, reading pump current and you will see lots of little spikes as the whiskers and flakes get dislodged. Wear hearing protection because you will need to do this for about 20 minutes. When you stop seeing any spike in ion current with hammer whacks, you are done.
2) Vent the system, pump it down, and restart the ion pump prematurely with over current protection turned off. The pump should get hot. That's basically a poor man's bake. It works.
3) Hi-pot. This is a technique where the service engineer brings in a high current ~10KV power supply and connects it to the pump for about an hour. The high voltage and high current blast all the whiskers and flakes quite nicely. However, if you have never done this yourself, get the service engineer to do it and be paranoid because it is VERY DANGEROUS! If the standard power supply for ion pumps wasn't dangerous enough, this is one that should treated with the greatest caution.
John Mardinly, Intel
This is not necessarily an opinion of Intel Corporation
-----Original Message----- X-from: beaurega-at-westol.com [mailto:beaurega-at-westol.com] Sent: Thursday, January 31, 2008 9:25 AM To: Mardinly, John
Hi,
My IGP on my CM series TEM lasted 11-13 years. 4 years is quite short., IMO.
If you think you have carbon or hydrocarbon issues, have your serviceman check the long column tubing liner just under the anode cap for a black coating of residue that builds up on the liner's inner wall. There should be a tool in the factory tool kit to remove this tubing liner. Look at the amount of the deposit and the color. Ask the serviceman if that amount of deposit seems normal or excessive. I would have a supply of 6-8 inch long wooden "Q-tip swabs" and Pol polish handy for him.
HTH,
Paul
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==============================Original Headers============================== 19, 32 -- From john.mardinly-at-intel.com Fri Feb 1 14:48:11 2008 19, 32 -- Received: from mga11.intel.com (mga11.intel.com [192.55.52.93]) 19, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m11KmAUv022712 19, 32 -- for {Microscopy-at-msa.microscopy.com} ; Fri, 1 Feb 2008 14:48:11 -0600 19, 32 -- Received: from fmsmga001.fm.intel.com ([10.253.24.23]) 19, 32 -- by fmsmga102.fm.intel.com with ESMTP; 01 Feb 2008 12:45:49 -0800 19, 32 -- X-ExtLoop1: 1 19, 32 -- X-IronPort-AV: E=Sophos;i="4.25,292,1199692800"; 19, 32 -- d="scan'208";a="511891848" 19, 32 -- Received: from orsmsx334.jf.intel.com ([10.22.226.45]) 19, 32 -- by fmsmga001.fm.intel.com with ESMTP; 01 Feb 2008 12:48:10 -0800 19, 32 -- Received: from orsmsx423.amr.corp.intel.com ([10.22.226.104]) by orsmsx334.jf.intel.com with Microsoft SMTPSVC(6.0.3790.1830); 19, 32 -- Fri, 1 Feb 2008 12:47:57 -0800 19, 32 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 19, 32 -- Content-class: urn:content-classes:message 19, 32 -- MIME-Version: 1.0 19, 32 -- Content-Type: text/plain; 19, 32 -- charset="us-ascii" 19, 32 -- Subject: RE: [Microscopy] Re: ion pump longevity 19, 32 -- Date: Fri, 1 Feb 2008 12:47:56 -0800 19, 32 -- Message-ID: {36C438BD1408B1499A19BA5730AAFC63BB8886-at-orsmsx423.amr.corp.intel.com} 19, 32 -- X-MS-Has-Attach: 19, 32 -- X-MS-TNEF-Correlator: 19, 32 -- Thread-Topic: [Microscopy] Re: ion pump longevity 19, 32 -- Thread-Index: AchkLze/C1E+7SDCT8eefxWk4GsNmwAxle8AAAeDmAA= 19, 32 -- References: {200801311724.m0VHObHi002733-at-ns.microscopy.com} 19, 32 -- From: "Mardinly, John" {john.mardinly-at-intel.com} 19, 32 -- To: {beaurega-at-westol.com} 19, 32 -- Cc: {Microscopy-at-msa.microscopy.com} 19, 32 -- X-OriginalArrivalTime: 01 Feb 2008 20:47:57.0722 (UTC) FILETIME=[B9DEA7A0:01C86513] 19, 32 -- Content-Transfer-Encoding: 8bit 19, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m11KmAUv022712 ==============================End of - Headers==============================
As usual Gary Brown is dead on with his comments. I agree that embedment is generally best avoided in these multilayer applications. We have seen swelling of the surface layer due to epoxy interaction as well, surprising some real polymer gurus around here. His specimen holder sounds elegant - I think I'll make one. We've taken a more down-and-dirty, and semi-disposable, approach here that may be useful in some of your applications.
We use some 15 mil (0.37 mm - thicker wouldn't hurt) high impact polystyrene (HIPS) stock that was lying around to form a specimen support by cutting a piece approximately twice as long as Gary cited, then folding it in half to form an open envelope. The specimen is sandwiched between the folded ends with the fold near the bottom of the chuck and the specimen just protruding from the open end of the sandwich, also just above the microtome jaws. Typically I will trim away the clamped envelope to form a little truncated pyramid at the top and trim the sample to project just above, such that almost all the sample is encased in the HIPS sandwich and the clamp. For specimens that need more support (i.e. very thin films), the disposable nature of this setup comes in handy. I trim away the entire assembly with the specimen inside to form a mesa with HIPS and sample on the face (this is especially helpful when cutting thick sections of multilayer films for light microscopy). The HIPS sides of the pyramid are beveled as well to keep the HIPS sectioning to a minimum. It is generally not a major problem to winnow the sections of interest away from the sectioned support material, but make sure you take the time to get to know the support material microstructure intimately just in case! Good luck!
Yours, Matt
Matthew Stephenson Impact Analytical/MMI 1910 W. Saint Andrews Rd. Midland, MI 48640 (989)832-5555 X506 stephenson-at-impactanalytical.com
-----Original Message----- X-from: gary.m.brown-at-exxonmobil.com [mailto:gary.m.brown-at-exxonmobil.com] Sent: Thursday, January 31, 2008 11:19 AM To: stephenson-at-impactanalytical.com
Sandra,
I agree with Jessica that you should use cryomicrotomy and collect dry sections. This is a challenging technique but with practice you will find it to be valuable. Microtome vendors and other training facilities offer workshops on the cryomicrotomy.
In our lab we use a 'plastic chuck' for sectioning films at ambient and low temperature. The chuck consists of a piece of plastic with approximate dimensions of 4 mm x 7 mm x 3 mm (width x length x thickness). These dimensions should be set to your personal preference and to fit your sample and microtome chuck. Use a stiff plastic such as high density polyethylene or polypropylene that will hold the sample tight but is not rigid or inflexible. Using a sharp razor blade, bisect the 3 mm thickness, cutting through nearly the full length of the plastic piece and leaving a couple of millimeters of at the other end of the piece to hold the arms of the chuck together. The end results is a Y-shaped piece of plastic (see diagram below). To use the chuck, slide the film between the arms of the plastic chuck leaving less than 1 mm exposed; the more film is exposed, the more likely it is to move side-to-side during microtomy. Listers may contact me off-line for a drawing of the chuck.
I recommend that you do not embed films in epoxy if at all possible. The embedding medium, whatever it might be, only contributes to the difficulty of cutting. The problem is that the film has several layers, each with different cutting properties at low temperature; the problem is often even worse at ambient temperature. Adding the embedding medium contributes another medium that most probably has very different cutting properties than any material in the film.
Disclaimer: The comments and opinions given above are those of the author alone and do not represent any position of ExxonMobil Chemical Company.
Regards,
Gary M. Brown Microscopy Specialist ExxonMobil Chemical Company Baytown Technology & Engineering - West 5200 Bayway Drive Baytown, Texas 77520-2101 phone: 281 834 2387 fax: 281 834 2395 e-mail: Gary.M.Brown-at-ExxonMobil.com
"Our business in life is not to succeed, but to continue to fail in good spirits." Robert Louis Stevenson
cervantes-at-bend res.com To gary.m.brown-at-exxonmobil.com 01/30/08 07:40 cc PM Subject [Microscopy] RE: viaWWW: 2 questions Please respond concerning microtomy of thin films to cervantes-at-bend res.com
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Sandra -
I also work with water soluble materials, and so often have to cut dry. I have found that a diamond knife is preferable to glass (less "sticky" so sections are easier to get off the knife surface), but that may be something you want to experiment with. To transfer sections from the knife to the grid, I use an eyelash probe (I buy mine from Ted Pella, but people make them as well). It takes a little practice, but that is what works best for me. I also use the probe to gently "flatten" the sections onto the grid surface (I use 300 mesh Copper grids with Formvar support film).
Diatome used to make a Cryo P diamond knife (don't know if they still do) with a special platform that makes it easier to transfer sections. This has been my favorite knife for dry-sectioning.
Sorry can't help with your second question, but hope this was useful.
Jessica
____________________ Jessica Cervantes Bend Research Inc 64550 Research Rd Bend, OR 97701 www.bendres.com
-----Original Message----- X-from: sandra.gardner-at-xerox.com [mailto:sandra.gardner-at-xerox.com] Sent: Wednesday, January 30, 2008 2:49 PM To: Cervantes, Jessica
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Title-Subject: [Filtered] 2 questions concerning microtomy of thin films
Question: I have a multi layer film in which one of the layers is water soluble. We want to cross section the sample for TEM to view the various layers, each of which are less than 500nm thick. The total thickness of the plastic substrate plus multilayer film is aprox. 500microns (the substate is aprox 300microns of this). I've embedded the film in a epoxy resin for sectioning. I don't know how to go about capturing thin sections ( {100nm) if I can't put water in the boat. I could cut them dry, but again, I'm not sure how to transfer them to the grid. I am using a Diatome diamond knife. Any suggestions would be greatly appreciated.
Another question I have regards the use of tape as a support media for cryo-sectioning. Many of our samples are films which I typically embed in epoxy resin. We now have cryo-sectioning capabilities and i would like to simply sandwich my film between tape and cryo cut thin films for TEM analyses. Is there any tape which has good adhesion properties at -120C and stand up well for TEM (glue does not interfere with sample)
Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (yashaa-at-hotmail.com) from http://www.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Friday, February 1, 2008 at 16:42:01 ---------------------------------------------------------------------------
Email: yashaa-at-hotmail.com Name: Rasha ElBashir
Organization: Aachen university of applied science
Education: Graduate College
Location: Aachen, Germany
Question: I need some information for my study about the scanning transmission electron microscope (STEM), how does it work, what is it's principle and other basic information
Please Google "scanning transmission electron microscope" and within the first 10 entries you will be off to a great start.
Roseann
On Feb 1, 2008, at 3:00 PM, yashaa-at-hotmail.com wrote:
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==============================Original Headers============================== 6, 26 -- From RCsencsits-at-lbl.gov Fri Feb 1 17:18:07 2008 6, 26 -- Received: from ironport1.lbl.gov (ironport1.lbl.gov [128.3.41.47]) 6, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m11NI6Wj000454 6, 26 -- for {microscopy-at-microscopy.com} ; Fri, 1 Feb 2008 17:18:06 -0600 6, 26 -- X-Ironport-SBRS: None 6, 26 -- X-IronPort-Anti-Spam-Filtered: true 6, 26 -- X-IronPort-Anti-Spam-Result: Ao8CAAo3o0eAAykY/2dsb2JhbACuZQ 6, 26 -- X-IronPort-AV: E=Sophos;i="4.25,292,1199692800"; 6, 26 -- d="scan'208";a="87803098" 6, 26 -- Received: from mta1.lbl.gov ([128.3.41.24]) 6, 26 -- by ironport1.lbl.gov with ESMTP; 01 Feb 2008 15:18:05 -0800 6, 26 -- Received: from [131.243.35.239] (0-17-f2-2d-d1-b7.dhcp.lbl.gov [131.243.35.239]) 6, 26 -- by mta1.lbl.gov (8.13.8/8.13.8) with ESMTP id m11NI5QS016516; 6, 26 -- Fri, 1 Feb 2008 15:18:05 -0800 (PST) 6, 26 -- Cc: microscopy-at-microscopy.com 6, 26 -- Message-Id: {5F1C4471-F05A-4969-801F-E3457F60C30D-at-lbl.gov} 6, 26 -- From: Roseann Csencsits {RCsencsits-at-lbl.gov} 6, 26 -- To: yashaa-at-hotmail.com 6, 26 -- In-Reply-To: {200802012300.m11N0r1G031688-at-ns.microscopy.com} 6, 26 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 6, 26 -- Content-Transfer-Encoding: 7bit 6, 26 -- Mime-Version: 1.0 (Apple Message framework v915) 6, 26 -- Subject: Re: [Microscopy] AskAMicroscopist: info on scanning transmission electron 6, 26 -- Date: Fri, 1 Feb 2008 15:18:04 -0800 6, 26 -- References: {200802012300.m11N0r1G031688-at-ns.microscopy.com} 6, 26 -- X-Mailer: Apple Mail (2.915) ==============================End of - Headers==============================
There is also a good overview on the FEI site. You can download "All you wanted to know about Electron MIcrosopy.... but didn't dare to ask."
Caveat: MME and The MIP have no commercial interest.
Hope this is helpful. Barbara Foster, President
Microscopy/Microscopy Education 7101 Royal Glen Trail, Suite A McKinney TX 75070 P: (972)924-5310 Skype: fostermme W: www.MicroscopyEducation.com
MME is now scheduling customized, on-site courses through July 2008. Call us today for details.
We are sorry to report that Optimizing Light Microscopy for the Biological and Clinical Laboratory is no longer available.
At 05:29 PM 2/1/2008, RCsencsits-at-lbl.gov wrote:
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Mine is manual insertion and retraction of the solid state STEM detector('s) diodes. The top side of the detector has four diodes and another underneath. Switching amongst the diodes provides BF and DF STEM.
Specimens are held in a standard 3mm diameter TEM grid and are typically imaged at 30KV for highest resolution. Instead of a constant beam of electrons flooding the specimen (like a TEM), the STEM does electron beam scanning of the specimen. Quite a different approach. Sometimes, very good and interesting results.
gary g.
At 02:52 PM 2/1/2008, you wrote:
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==============================Original Headers============================== 9, 21 -- From gary-at-gaugler.com Fri Feb 1 17:52:57 2008 9, 21 -- Received: from smtp2.mc.surewest.net (qsmtp.mc.surewest.net [66.60.130.145]) 9, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m11NqvpY026274 9, 21 -- for {microscopy-at-microscopy.com} ; Fri, 1 Feb 2008 17:52:57 -0600 9, 21 -- Message-Id: {200802012352.m11NqvpY026274-at-ns.microscopy.com} 9, 21 -- Received: (qmail 23015 invoked from network); 1 Feb 2008 15:52:56 -0800 9, 21 -- Received: by simscan 1.1.0 ppid: 23012, pid: 23013, t: 0.0776s 9, 21 -- scanners: regex: 1.1.0 attach: 1.1.0 9, 21 -- Received: from unknown (HELO thor.gaugler.com) (66.60.171.211) 9, 21 -- by smtp2 with SMTP; 1 Feb 2008 15:52:56 -0800 9, 21 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 9, 21 -- Date: Fri, 01 Feb 2008 15:52:54 -0800 9, 21 -- To: yashaa-at-hotmail.com 9, 21 -- From: Gary Gaugler {gary-at-gaugler.com} 9, 21 -- Subject: Re: [Microscopy] AskAMicroscopist: info on scanning 9, 21 -- transmission electron 9, 21 -- Cc: MSA listserver {microscopy-at-microscopy.com} 9, 21 -- In-Reply-To: {200802012252.m11Mq68E022133-at-ns.microscopy.com} 9, 21 -- References: {200802012252.m11Mq68E022133-at-ns.microscopy.com} 9, 21 -- Mime-Version: 1.0 9, 21 -- Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-48833211 ==============================End of - Headers==============================
I learned about the plastic microtomy chuck from Lou Ban, a microscopist who worked at Exxon Chemical some time ago. I've used it for +20 years with success. Thanks, Lou.
You make good points regarding the specifics of use of the chuck during microtomy. Your disposable chuck is ideal for the case in which a cross-section of a thin film is needed for SEM/EDS. After cryo-sectioning or cryo-facing (cryo-planing), the chuck/film assembly can be transferred directly to the SEM for analysis.
We all have insight into what makes a better mousetrap, or in this case, a microtomy chuck. The folks in our lab over the years used the chuck with variations that fit their tastes and needs. Without folk's new ideas and modifications of old ones, man wouldn't be where he is today.
Regards,
Gary M. Brown Microscopy Specialist ExxonMobil Chemical Company Baytown Technology & Engineering - West 5200 Bayway Drive Baytown, Texas 77520-2101 phone: 281 834 2387 fax: 281 834 2395 e-mail: Gary.M.Brown-at-ExxonMobil.com
"Our business in life is not to succeed, but to continue to fail in good spirits." Robert Louis Stevenson
stephenson-at-imp actanalytical. com To gary.m.brown-at-exxonmobil.com cc 02/01/08 03:51 PM Subject [Microscopy] RE: viaWWW: 2 questions concerning microtomy of Please respond thi to stephenson-at-imp actanalytical. com
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Greetings Sandra,
As usual Gary Brown is dead on with his comments. I agree that embedment is generally best avoided in these multilayer applications. We have seen swelling of the surface layer due to epoxy interaction as well, surprising some real polymer gurus around here. His specimen holder sounds elegant - I think I'll make one. We've taken a more down-and-dirty, and semi-disposable, approach here that may be useful in some of your applications.
We use some 15 mil (0.37 mm - thicker wouldn't hurt) high impact polystyrene (HIPS) stock that was lying around to form a specimen support by cutting a piece approximately twice as long as Gary cited, then folding it in half to form an open envelope. The specimen is sandwiched between the folded ends with the fold near the bottom of the chuck and the specimen just protruding from the open end of the sandwich, also just above the microtome jaws. Typically I will trim away the clamped envelope to form a little truncated pyramid at the top and trim the sample to project just above, such that almost all the sample is encased in the HIPS sandwich and the clamp. For specimens that need more support (i.e. very thin films), the disposable nature of this setup comes in handy. I trim away the entire assembly with the specimen inside to form a mesa with HIPS and sample on the face (this is especially helpful when cutting thick sections of multilayer films for light microscopy). The HIPS sides of the pyramid are beveled as well to keep the HIPS sectioning to a minimum. It is generally not a major problem to winnow the sections of interest away from the sectioned support material, but make sure you take the time to get to know the support material microstructure intimately just in case! Good luck!
Yours, Matt
Matthew Stephenson Impact Analytical/MMI 1910 W. Saint Andrews Rd. Midland, MI 48640 (989)832-5555 X506 stephenson-at-impactanalytical.com
-----Original Message----- X-from: gary.m.brown-at-exxonmobil.com [mailto:gary.m.brown-at-exxonmobil.com] Sent: Thursday, January 31, 2008 11:19 AM To: stephenson-at-impactanalytical.com
Sandra,
I agree with Jessica that you should use cryomicrotomy and collect dry sections. This is a challenging technique but with practice you will find it to be valuable. Microtome vendors and other training facilities offer workshops on the cryomicrotomy.
In our lab we use a 'plastic chuck' for sectioning films at ambient and low temperature. The chuck consists of a piece of plastic with approximate dimensions of 4 mm x 7 mm x 3 mm (width x length x thickness). These dimensions should be set to your personal preference and to fit your sample and microtome chuck. Use a stiff plastic such as high density polyethylene or polypropylene that will hold the sample tight but is not rigid or inflexible. Using a sharp razor blade, bisect the 3 mm thickness, cutting through nearly the full length of the plastic piece and leaving a couple of millimeters of at the other end of the piece to hold the arms of the chuck together. The end results is a Y-shaped piece of plastic (see diagram below). To use the chuck, slide the film between the arms of the plastic chuck leaving less than 1 mm exposed; the more film is exposed, the more likely it is to move side-to-side during microtomy. Listers may contact me off-line for a drawing of the chuck.
I recommend that you do not embed films in epoxy if at all possible. The embedding medium, whatever it might be, only contributes to the difficulty of cutting. The problem is that the film has several layers, each with different cutting properties at low temperature; the problem is often even worse at ambient temperature. Adding the embedding medium contributes another medium that most probably has very different cutting properties than any material in the film.
Disclaimer: The comments and opinions given above are those of the author alone and do not represent any position of ExxonMobil Chemical Company.
Regards,
Gary M. Brown Microscopy Specialist ExxonMobil Chemical Company Baytown Technology & Engineering - West 5200 Bayway Drive Baytown, Texas 77520-2101 phone: 281 834 2387 fax: 281 834 2395 e-mail: Gary.M.Brown-at-ExxonMobil.com
"Our business in life is not to succeed, but to continue to fail in good spirits." Robert Louis Stevenson
cervantes-at-bend res.com To gary.m.brown-at-exxonmobil.com 01/30/08 07:40 cc PM Subject [Microscopy] RE: viaWWW: 2 questions Please respond concerning microtomy of thin films to cervantes-at-bend res.com
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Sandra -
I also work with water soluble materials, and so often have to cut dry. I have found that a diamond knife is preferable to glass (less "sticky" so sections are easier to get off the knife surface), but that may be something you want to experiment with. To transfer sections from the knife to the grid, I use an eyelash probe (I buy mine from Ted Pella, but people make them as well). It takes a little practice, but that is what works best for me. I also use the probe to gently "flatten" the sections onto the grid surface (I use 300 mesh Copper grids with Formvar support film).
Diatome used to make a Cryo P diamond knife (don't know if they still do) with a special platform that makes it easier to transfer sections. This has been my favorite knife for dry-sectioning.
Sorry can't help with your second question, but hope this was useful.
Jessica
____________________ Jessica Cervantes Bend Research Inc 64550 Research Rd Bend, OR 97701 www.bendres.com
-----Original Message----- X-from: sandra.gardner-at-xerox.com [mailto:sandra.gardner-at-xerox.com] Sent: Wednesday, January 30, 2008 2:49 PM To: Cervantes, Jessica
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html ------------------------------------------------------------------------ --- Remember this posting is most likely not from a Subscriber, so when replying please copy both sandra.gardner-at-xerox.com as well as the MIcroscopy Listserver ------------------------------------------------------------------------ ---
Title-Subject: [Filtered] 2 questions concerning microtomy of thin films
Question: I have a multi layer film in which one of the layers is water soluble. We want to cross section the sample for TEM to view the various layers, each of which are less than 500nm thick. The total thickness of the plastic substrate plus multilayer film is aprox. 500microns (the substate is aprox 300microns of this). I've embedded the film in a epoxy resin for sectioning. I don't know how to go about capturing thin sections ( {100nm) if I can't put water in the boat. I could cut them dry, but again, I'm not sure how to transfer them to the grid. I am using a Diatome diamond knife. Any suggestions would be greatly appreciated.
Another question I have regards the use of tape as a support media for cryo-sectioning. Many of our samples are films which I typically embed in epoxy resin. We now have cryo-sectioning capabilities and i would like to simply sandwich my film between tape and cryo cut thin films for TEM analyses. Is there any tape which has good adhesion properties at -120C and stand up well for TEM (glue does not interfere with sample)
And if things are so bad then on have to try some of the tricks presented by John, here are two more :
-make a E-4 to E-6 O2 inlet in the pumpe while it's working. It may refresh the Ti surface, by O2 ion reactive etching. Not easy to preform on a microscope. One must have a leakvalve on the scope.
-make a vacuum in the 10E1 - 10 mbar range under air. Same effect that former trick, but with more pressure and not pure oxygen. It's a DC glow discharge. On a microscope it needs to put the vacuum control board in manual mode, and to short some securities... The pump will warm up too. One have together "baking" and ion etching.
Jacques
J. Faerber IPCMS-GSI (Institut de Physique et Chimie des Matériaux de Strasbourg Groupe Surface et Interfaces) 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
john.mardinly-at-intel.com a écrit : } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } There are three little tricks for extending the life of ion pumps that } have not been mentioned yet that I thought I could contribute based on } years of experience with JEOL and Topcon TEM's: } } 1) The hammer. Yes, just smack the bajeezus out of the ion pump body } (not the magnet) with a hammer. The shock knocks most of the whiskers } and flakes loose. Do it with the emitter cold, because it will shock the } whole TEM. Put emphasis on a large number of mild whacks in every } direction of the pump housing. Keep the pump HV on, reading pump current } and you will see lots of little spikes as the whiskers and flakes get } dislodged. Wear hearing protection because you will need to do this for } about 20 minutes. When you stop seeing any spike in ion current with } hammer whacks, you are done. } } 2) Vent the system, pump it down, and restart the ion pump prematurely } with over current protection turned off. The pump should get hot. That's } basically a poor man's bake. It works. } } 3) Hi-pot. This is a technique where the service engineer brings in a } high current ~10KV power supply and connects it to the pump for about an } hour. The high voltage and high current blast all the whiskers and } flakes quite nicely. However, if you have never done this yourself, get } the service engineer to do it and be paranoid because it is VERY } DANGEROUS! If the standard power supply for ion pumps wasn't dangerous } enough, this is one that should treated with the greatest caution. } } John Mardinly, Intel } } This is not necessarily an opinion of Intel Corporation } } -----Original Message----- } X-from: beaurega-at-westol.com [mailto:beaurega-at-westol.com] } Sent: Thursday, January 31, 2008 9:25 AM } To: Mardinly, John } Subject: [Microscopy] Re: ion pump longevity } } } } } ------------------------------------------------------------------------ } ---- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ------------------------------------------------------------------------ } ---- } } Hi, } } My IGP on my CM series TEM lasted 11-13 years. 4 years is quite short., } IMO. } } If you think you have carbon or hydrocarbon issues, have your serviceman } check the long column tubing liner just under the anode cap for a black } coating of residue that builds up on the liner's inner wall. There } should } be a tool in the factory tool kit to remove this tubing liner. Look at } the } amount of the deposit and the color. Ask the serviceman if that amount } of } deposit seems normal or excessive. I would have a supply of 6-8 inch } long } wooden "Q-tip swabs" and Pol polish handy for him. } } HTH, } } Paul } } At 09:01 AM 1/30/08 -0600, you wrote: } } } } } ----------------------------------------------------------------------- } } } ----- } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } } America } } } To Subscribe/Unsubscribe -- } } } http://www.microscopy.com/MicroscopyListserver } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ----------------------------------------------------------------------- } } } ----- } } } Dear listers, } } } } We just changed the IGP pump of the gun chamber only 4 years after the } } } installation of our Tecnai G20. This change was advised by a FEI } engineer. } } } We do not even have 400 working hours on this machine and I wondered if } } } it } was normal that we already have to change the pump. } } } We work mainly with ultrathin sections of biological probes embedded in } } } Epon. They are pretty contaminated with carbon, as evidenced by light } circles remaining on the sections after even a very short illumination } time } (I am usually working at 120 kV, LaB6). I am wondering if this carbon } contamination, evaporated by the electron beam, is not at least partly } responsible for the dirtiness of the pumps (the pump of the column is } bigger than the one for the gun, which would explain why it was not so } dirty). If this is the case, perhaps a plasma cleaner would not only be } a } convenience for me but could bring a financial advantage for my boss (I } know you see what I mean ;-)). } } } What would be the cost of a plasma cleaner? } } What is your opinion on the question? Do you think that a plasma } } } cleaner } would increase the lifetime of the IGPs? } } } Best regards, } } } } Stephane } } } } } } } } } ________________________________________________________________________ } ____ } ________ } } } Looking for last minute shopping deals? } } Find them fast with Yahoo! Search. } } } http://tools.search.yahoo.com/newsearch/category.php?category=shopping } } } ==============================Original } } } Headers============================== } } } 6, 18 -- From nizets2-at-yahoo.com Wed Jan 30 09:00:54 2008 } } 6, 18 -- Received: from web37402.mail.mud.yahoo.com } } } (web37402.mail.mud.yahoo.com [209.191.91.134]) } } } 6, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP } } } id } m0UF0pGt005637 } } } 6, 18 -- for {microscopy-at-microscopy.com} ; Wed, 30 Jan 2008 } } } 09:00:53 -0600 } } } 6, 18 -- Received: (qmail 99976 invoked by uid 60001); 30 Jan 2008 } } } 15:00:50 -0000 } } } 6, 18 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; } } 6, 18 -- s=s1024; d=yahoo.com; } } 6, 18 -- } } } h=X-YMail-OSG:Received:X-Mailer:Date:From:To:MIME-Version:Content-Type:M } essa } ge-ID; } } } 6, 18 -- } } } b=GhQVB0xX2vcU83GfwvaZw+ifHUliA8IiLQHTZzdx3l1eUcyH0/480eo0thBMxgf3UWIzP9 } wOTj } IMLyE3Vcuk+F+LF9WdARAeAKeteL2+M8tArqzjD+Ms3FCyb8Qutj6M6tE0fcvz6TzcAreJXf } nLKD } hyAOWq+S20/9pUAEHFJq8=; } } } 6, 18 -- X-YMail-OSG: } } } OzrDwaUVM1lgyKl4Xyckx0JtBXwejlTzTn4PS5.ANAmKzTTfIP7HLGPXkbnY_W4GzDVe.Mr3 } Ifmh } gAzdLAg6vS83QQMBWKgdGjifx9pPsyBVYWmv4Mk- } } } 6, 18 -- Received: from [80.122.101.100] by web37402.mail.mud.yahoo.com } } } via HTTP; Wed, 30 Jan 2008 07:00:50 PST } } } 6, 18 -- X-Mailer: YahooMailRC/818.31 YahooMailWebService/0.7.162 } } 6, 18 -- Date: Wed, 30 Jan 2008 07:00:50 -0800 (PST) } } 6, 18 -- From: Stephane Nizet {nizets2-at-yahoo.com} } } 6, 18 -- To: microscopy-at-microscopy.com } } 6, 18 -- MIME-Version: 1.0 } } 6, 18 -- Content-Type: text/plain; charset=us-ascii } } 6, 18 -- Message-ID: {440079.99572.qm-at-web37402.mail.mud.yahoo.com} } } ==============================End of - } } } Headers============================== } } } } } } } ==============================Original } Headers============================== } 6, 16 -- From beaurega-at-westol.com Thu Jan 31 11:24:28 2008 } 6, 16 -- Received: from mail.winbeam.com (mail.winbeam.com } [64.84.96.10]) } 6, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP } id m0VHOSca002606 } 6, 16 -- for {microscopy-at-microscopy.com} ; 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==============================Original Headers============================== 10, 29 -- From jacques.faerber-at-ipcms.u-strasbg.fr Mon Feb 4 07:01:57 2008 10, 29 -- Received: from mailhost.u-strasbg.fr (mailhost.u-strasbg.fr [130.79.200.151]) 10, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m14D1uSU031600 10, 29 -- for {microscopy-at-microscopy.com} ; Mon, 4 Feb 2008 07:01:56 -0600 10, 29 -- Received: from ipcms.u-strasbg.fr (ipcms.u-strasbg.fr [130.79.210.2]) 10, 29 -- by mailhost.u-strasbg.fr (8.13.8/jtpda-5.5pre1) with ESMTP id m14D1qDv064694 10, 29 -- for {microscopy-at-microscopy.com} ; Mon, 4 Feb 2008 14:01:52 +0100 (CET) 10, 29 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr [130.79.152.3]) 10, 29 -- by ipcms.u-strasbg.fr (Postfix) with ESMTP id 49FFD107CE5F 10, 29 -- for {Microscopy-at-Microscopy.Com} ; Mon, 4 Feb 2008 14:01:12 +0100 (CET) 10, 29 -- Message-ID: {47A70C99.7080603-at-ipcms.u-strasbg.fr} 10, 29 -- Date: Mon, 04 Feb 2008 14:01:13 +0100 10, 29 -- From: "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr} 10, 29 -- User-Agent: Thunderbird 1.5.0.14pre (X11/20071022) 10, 29 -- MIME-Version: 1.0 10, 29 -- To: Microscopy-at-microscopy.com 10, 29 -- Subject: Re: [Microscopy] ion pump longevity more 10, 29 -- References: {200802012057.m11Kv7On031382-at-ns.microscopy.com} 10, 29 -- In-Reply-To: {200802012057.m11Kv7On031382-at-ns.microscopy.com} 10, 29 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 10, 29 -- Content-Transfer-Encoding: 8bit 10, 29 -- X-IPCMS-MailScanner: Found to be clean 10, 29 -- X-IPCMS-MailScanner-From: jacques.faerber-at-ipcms.u-strasbg.fr 10, 29 -- X-Greylist: Sender IP whitelisted, not delayed by milter-greylist-3.0 (mailhost.u-strasbg.fr [130.79.200.151]); Mon, 04 Feb 2008 14:01:52 +0100 (CET) 10, 29 -- X-Virus-Scanned: ClamAV 0.88.7/5678/Mon Feb 4 02:15:53 2008 on mr1.u-strasbg.fr 10, 29 -- X-Virus-Status: Clean 10, 29 -- X-Spam-Status: No, score=-0.1 required=5.0 tests=AWL autolearn=disabled 10, 29 -- version=3.1.8 10, 29 -- X-Spam-Checker-Version: SpamAssassin 3.1.8 (2007-02-13) on mr1.u-strasbg.fr ==============================End of - Headers==============================
Sorry for the oups! I've forgotten the unit for the first trick, one has to read "E-4 to E-6 mbar O2 (oxygene)". And a little "-" is missing in the second one " 10E-1 to 10 mbar".
It's not a good thing to answer to quickly !
And if things are so bad then on have to try some of the tricks presented by John, here are two more :
-make a E-4 to E-6 mabr O2 inlet in the pump while it's working. It may refresh the Ti surface, by O2 ion reactive etching. Not easy to perform on a microscope. One must have a leakvalve on the scope.
-make a vacuum in the 10E-1 - 10 mbar range under air. Same effect that former trick, but with more pressure and not pure oxygen. It's a DC glow discharge. On a microscope it needs to put the vacuum control board in manual mode, and to short some securities... The pump will warm up too. One have together "baking" and ion etching.
Jacques
J. Faerber IPCMS-GSI (Institut de Physique et Chimie des Matériaux de Strasbourg Groupe Surface et Interfaces) 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
john.mardinly-at-intel.com a écrit : } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } There are three little tricks for extending the life of ion pumps that } have not been mentioned yet that I thought I could contribute based on } years of experience with JEOL and Topcon TEM's: } } 1) The hammer. Yes, just smack the bajeezus out of the ion pump body } (not the magnet) with a hammer. The shock knocks most of the whiskers } and flakes loose. Do it with the emitter cold, because it will shock the } whole TEM. Put emphasis on a large number of mild whacks in every } direction of the pump housing. Keep the pump HV on, reading pump current } and you will see lots of little spikes as the whiskers and flakes get } dislodged. Wear hearing protection because you will need to do this for } about 20 minutes. When you stop seeing any spike in ion current with } hammer whacks, you are done. } } 2) Vent the system, pump it down, and restart the ion pump prematurely } with over current protection turned off. The pump should get hot. That's } basically a poor man's bake. It works. } } 3) Hi-pot. This is a technique where the service engineer brings in a } high current ~10KV power supply and connects it to the pump for about an } hour. The high voltage and high current blast all the whiskers and } flakes quite nicely. However, if you have never done this yourself, get } the service engineer to do it and be paranoid because it is VERY } DANGEROUS! If the standard power supply for ion pumps wasn't dangerous } enough, this is one that should treated with the greatest caution. } } John Mardinly, Intel } } This is not necessarily an opinion of Intel Corporation } } -----Original Message----- } X-from: beaurega-at-westol.com [mailto:beaurega-at-westol.com] } Sent: Thursday, January 31, 2008 9:25 AM } To: Mardinly, John } Subject: [Microscopy] Re: ion pump longevity } } } } } ------------------------------------------------------------------------ } ---- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ------------------------------------------------------------------------ } ---- } } Hi, } } My IGP on my CM series TEM lasted 11-13 years. 4 years is quite short., } IMO. } } If you think you have carbon or hydrocarbon issues, have your serviceman } check the long column tubing liner just under the anode cap for a black } coating of residue that builds up on the liner's inner wall. There } should } be a tool in the factory tool kit to remove this tubing liner. Look at } the } amount of the deposit and the color. Ask the serviceman if that amount } of } deposit seems normal or excessive. I would have a supply of 6-8 inch } long } wooden "Q-tip swabs" and Pol polish handy for him. } } HTH, } } Paul } } At 09:01 AM 1/30/08 -0600, you wrote: } } } } } ----------------------------------------------------------------------- } } } ----- } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } } America } } } To Subscribe/Unsubscribe -- } } } http://www.microscopy.com/MicroscopyListserver } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ----------------------------------------------------------------------- } } } ----- } } } Dear listers, } } } } We just changed the IGP pump of the gun chamber only 4 years after the } } } installation of our Tecnai G20. This change was advised by a FEI } engineer. } } } We do not even have 400 working hours on this machine and I wondered if } } } it } was normal that we already have to change the pump. } } } We work mainly with ultrathin sections of biological probes embedded in } } } Epon. They are pretty contaminated with carbon, as evidenced by light } circles remaining on the sections after even a very short illumination } time } (I am usually working at 120 kV, LaB6). I am wondering if this carbon } contamination, evaporated by the electron beam, is not at least partly } responsible for the dirtiness of the pumps (the pump of the column is } bigger than the one for the gun, which would explain why it was not so } dirty). If this is the case, perhaps a plasma cleaner would not only be } a } convenience for me but could bring a financial advantage for my boss (I } know you see what I mean ;-)). } } } What would be the cost of a plasma cleaner? } } What is your opinion on the question? Do you think that a plasma } } } cleaner } would increase the lifetime of the IGPs? } } } Best regards, } } } } Stephane } } } } } } } } } ________________________________________________________________________ } ____ } ________ } } } Looking for last minute shopping deals? } } Find them fast with Yahoo! Search. } } } http://tools.search.yahoo.com/newsearch/category.php?category=shopping } } } ==============================Original } } } Headers============================== } } } 6, 18 -- From nizets2-at-yahoo.com Wed Jan 30 09:00:54 2008 } } 6, 18 -- Received: from web37402.mail.mud.yahoo.com } } } (web37402.mail.mud.yahoo.com [209.191.91.134]) } } } 6, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP } } } id } m0UF0pGt005637 } } } 6, 18 -- for {microscopy-at-microscopy.com} ; Wed, 30 Jan 2008 } } } 09:00:53 -0600 } } } 6, 18 -- Received: (qmail 99976 invoked by uid 60001); 30 Jan 2008 } } } 15:00:50 -0000 } } } 6, 18 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; } } 6, 18 -- s=s1024; d=yahoo.com; } } 6, 18 -- } } } h=X-YMail-OSG:Received:X-Mailer:Date:From:To:MIME-Version:Content-Type:M } essa } ge-ID; } } } 6, 18 -- } } } b=GhQVB0xX2vcU83GfwvaZw+ifHUliA8IiLQHTZzdx3l1eUcyH0/480eo0thBMxgf3UWIzP9 } wOTj } IMLyE3Vcuk+F+LF9WdARAeAKeteL2+M8tArqzjD+Ms3FCyb8Qutj6M6tE0fcvz6TzcAreJXf } nLKD } hyAOWq+S20/9pUAEHFJq8=; } } } 6, 18 -- X-YMail-OSG: } } } OzrDwaUVM1lgyKl4Xyckx0JtBXwejlTzTn4PS5.ANAmKzTTfIP7HLGPXkbnY_W4GzDVe.Mr3 } Ifmh } gAzdLAg6vS83QQMBWKgdGjifx9pPsyBVYWmv4Mk- } } } 6, 18 -- Received: from [80.122.101.100] by web37402.mail.mud.yahoo.com } } } via HTTP; Wed, 30 Jan 2008 07:00:50 PST } } } 6, 18 -- X-Mailer: YahooMailRC/818.31 YahooMailWebService/0.7.162 } } 6, 18 -- Date: Wed, 30 Jan 2008 07:00:50 -0800 (PST) } } 6, 18 -- From: Stephane Nizet {nizets2-at-yahoo.com} } } 6, 18 -- To: microscopy-at-microscopy.com } } 6, 18 -- MIME-Version: 1.0 } } 6, 18 -- Content-Type: text/plain; charset=us-ascii } } 6, 18 -- Message-ID: {440079.99572.qm-at-web37402.mail.mud.yahoo.com} } } ==============================End of - } } } Headers============================== } } } } } } } ==============================Original } Headers============================== } 6, 16 -- From beaurega-at-westol.com Thu Jan 31 11:24:28 2008 } 6, 16 -- Received: from mail.winbeam.com (mail.winbeam.com } [64.84.96.10]) } 6, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP } id m0VHOSca002606 } 6, 16 -- for {microscopy-at-microscopy.com} ; Thu, 31 Jan 2008 } 11:24:28 -0600 } 6, 16 -- Received: (qmail 16520 invoked by uid 89); 31 Jan 2008 17:24:26 } -0000 } 6, 16 -- Received: from pitts-69-72-117-2.dynamic-dialup.coretel.net } (HELO millenium) (69.72.117.2) } 6, 16 -- by mail.winbeam.com with SMTP; 31 Jan 2008 17:24:26 -0000 } 6, 16 -- Message-Id: } {3.0.6.32.20080131122424.007b6420-at-pop3.norton.antivirus} } 6, 16 -- X-Sender: beaurega/mail.westol.com-at-pop3.norton.antivirus } (Unverified) } 6, 16 -- X-Mailer: QUALCOMM Windows Eudora Light Version 3.0.6 (32) } 6, 16 -- Date: Thu, 31 Jan 2008 12:24:24 -0500 } 6, 16 -- To: nizets2-at-yahoo.com, microscopy-at-microscopy.com } 6, 16 -- From: Beaurega {beaurega-at-westol.com} } 6, 16 -- Subject: Re: [Microscopy] ion pump longevity } 6, 16 -- Mime-Version: 1.0 } 6, 16 -- Content-Type: text/plain; charset="us-ascii" } ==============================End of - } Headers============================== } } } ==============================Original Headers============================== } 19, 32 -- From john.mardinly-at-intel.com Fri Feb 1 14:48:11 2008 } 19, 32 -- Received: from mga11.intel.com (mga11.intel.com [192.55.52.93]) } 19, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m11KmAUv022712 } 19, 32 -- for {Microscopy-at-msa.microscopy.com} ; 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-- J. Faerber IPCMS-GSI (Institut de Physique et Chimie des Matériaux de Strasbourg Groupe Surface et Interfaces) 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
I was asked by a biological microscopist for information pertaining to preparation and microtomy of polymer films and materials. Like many of us, we are being asked to broaden our area of expertise into unfamiliar territory. The following suggestions are based on guidance by several other microscopists and much experience over the years. I don't claim to be an expert, only a microscopist with a few proven tricks up my sleeve. This is not a comprehensive or authoritative monograph, just friendly advice. My hope is that this will cause a flurry of responses with additional suggestions and ideas, questions (and answers) on preparation of other sample types, and correction of any wrong statements by me.
Like biological microscopy, material science offers many challenges. The broad spectrum of materials often requires only several key preparation and microscopy tools and techniques. I would start with the materials that you are presented with and worry about other potential challenges when they arise. That said, there are several approaches to microtomy of materials that are worth mentioning. As in every other aspect of microscopy, sample preparation is absolutely essential.
Frankly, I learned most of the methods that I used by trial and error with help from a few colleagues. I'm sure that a several if not many good review papers and books deal with preparation techniques for catalyst-related materials such as zeolites, aluminas, silicas, etc. These are exceptionally important materials used to support catalyst species. The microtome vendors can provide much insight into preparation of materials for microscopy. Alternatively, other organizations such as McCrone Associates may offer workshop.
Consider polishing if larger flat surfaces of hard materials are needed for SEM analysis. Look for contract labs that will grind and polish your samples until you have a sufficiently high sample volume that you need to invest in grinding and polishing equipment.
Microtomy is the key to successful analysis of polymers. Several points follow: I generally try to avoid embedment. Exceptions to this are fibers, fabrics and tiny particles that require rigid supports during microtomy. When embedment is unavoidable, try to match the hardness of the medium to the sample as best possible. IMPORTANT: Do not cure any embedding medium at a temperature approaching the polymer melting point. If you don't know the melting temperature, use an ambient curing epoxy such as EpoFix. Polyolefins, a major class of polymers including polyethylene, polypropylene, etc., require special care since their melting temperatures can be as low as 40-50C or as high as 130-170C. Embedded or not, section the polymer at a temperature below it's glass transition temperature (Tg). When uncertain, lower the cutting temperature to { -130C. Cryo-section slowly since friction induced at higher rates can cause heating of the polymer above it's Tg where it will soften and plastically deform.
I use a standard approach to microtomy of hard materials like minerals and catalyst supports. Low viscosity is an important requirement for any embedding medium for small particles. I generally use LR White - hard grade - without the accelerator because it is has very low viscosity, is devoid of accelerator, is easy to use. The low viscosity allows it to readily infiltrate most catalyst support particles giving good embedment. Oven cure LR White at 80-90C for several hours in a nitrogen-purged oven. On occasions, I will use EpoFix epoxy. Call your supplier of embedding resins to find an epoxy:accelerator mixture that will give a hard block. Cure at as high a temperature as is recommended by the manufacturer or your supplier. If the material is not in a finely powered form and grinding permits, use a garnet mortar and pestle to finely divide the material. Sprinkle the powder toward one end of a flat embedding mold. Take care to have good density of particles in the mold but avoid piles or clumps of catalyst since they may not be well infiltrated with the embedding medium. Degas the embedding medium in a vacuum to remove bubbles and absorbed air. Epoxies and other higher viscosity embedding resins will take longer to degas than lower viscosity resins like LR White. Slowly layer the degassed embedding medium into the mold. Fully cover the sample and fill the mold. Identify the sample with a small paper label printed in pencil (ink runs when exposed to epoxy or LR White) in the opposite end of the mold from the sample. Place the mold plus catalyst into a vacuum and infiltrate for half an hour or so. If the embedding resin begins to foam due to release of air from within the powder, gently break vacuum several times until the material is stable in the vacuum. Heat cure in a nitrogen-purged oven for the required time.
Best regards,
Gary M. Brown Microscopy Specialist ExxonMobil Chemical Company Baytown Technology & Engineering - West 5200 Bayway Drive Baytown, Texas 77520-2101 phone: 281 834 2387 fax: 281 834 2395 e-mail: Gary.M.Brown-at-ExxonMobil.com
"Our business in life is not to succeed, but to continue to fail in good spirits." Robert Louis Stevenson
==============================Original Headers============================== 12, 18 -- From gary.m.brown-at-exxonmobil.com Mon Feb 4 10:24:49 2008 12, 18 -- Received: from hoespc02.exxonmobil.com (hoespc02.exxonmobil.com [158.35.223.2]) 12, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m14GOm4m030674 12, 18 -- for {microscopy-at-microscopy.com} ; Mon, 4 Feb 2008 10:24:49 -0600 12, 18 -- Received: from dalnmg04.na.xom.com (dalnmg04.na.xom.com [131.126.97.123]) 12, 18 -- by hoespc02.exxonmobil.com (Switch-3.1.11/Switch-3.1.11) with ESMTP id m14GOk97021184 12, 18 -- for {microscopy-at-microscopy.com} ; Mon, 4 Feb 2008 10:24:47 -0600 (CST) 12, 18 -- Subject: Microtomy of polymer films and hard particles 12, 18 -- Importance: 12, 18 -- To: microscopy-at-microscopy.com 12, 18 -- X-Mailer: Lotus Notes 652HF109 January 14, 2005 12, 18 -- Message-ID: {OFB8D03867.228FDDFC-ON862573E5.0058B16B-862573E5.005A27DB-at-exxonmobil.com} 12, 18 -- From: gary.m.brown-at-exxonmobil.com 12, 18 -- Date: Mon, 4 Feb 2008 10:24:44 -0600 12, 18 -- X-MIMETrack: Serialize by Router on Dalnmg04.na.xom.com/S/ExxonMobil(652FP1HF193|March 12, 18 -- 02, 2006) at 02/04/2008 10:24:47 AM 12, 18 -- MIME-Version: 1.0 12, 18 -- Content-type: text/plain; charset=US-ASCII ==============================End of - Headers==============================
We have a job opening for Director of the Imaging Technology Group ITG has a job opening for a new director.
The Beckman Institute at the University of Illinois, Urbana Champaign has a position opening for one (1) Director, Imaging Technology Group (ITG). The ITG's primary mission is to provide state-of-the-art imaging and visualization resources for researchers at the Institute and the University of Illinois. This mission is accomplished through two facilities: a Microscopy Suite, and a Visualization, Media, and Imaging Laboratory. A secondary mission of the ITG is to develop advanced technologies with an emphasis on projects in remote and virtual instrumentation. The ITG Director sets the vision for this group of approximately 25 staff members, writes grants and directs special projects to support that vision, and conducts daily management of the staff.
Read the announcement: http://www.itg.uiuc.edu/announcements/director.htm and if you have questions, please contact jobs-at-beckman.uiuc.edu.
Cheers,
Jonathan M. Ekman Imaging Technology Group Beckman Institute for Advanced Science and Technology University of Illinois at Urbana-Champaign 405 N. Mathews Avenue Urbana, IL 61801 USA Tel: 217-244-6292 Fax: 217-244-6219
==============================Original Headers============================== 7, 19 -- From jekman-at-uiuc.edu Mon Feb 4 14:11:24 2008 7, 19 -- Received: from expredir3.cites.uiuc.edu (expredir3.cites.uiuc.edu [128.174.5.186]) 7, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m14KBNju018540 7, 19 -- for {Microscopy-at-microscopy.com} ; Mon, 4 Feb 2008 14:11:23 -0600 7, 19 -- Received: from rollinsitg (rollins.itg.uiuc.edu [130.126.126.232]) 7, 19 -- by expredir3.cites.uiuc.edu (8.14.2/8.14.2) with ESMTP id m14KBMnq017277 7, 19 -- for {Microscopy-at-microscopy.com} ; Mon, 4 Feb 2008 14:11:22 -0600 (CST) 7, 19 -- From: "Jon Ekman" {jekman-at-uiuc.edu} 7, 19 -- To: {Microscopy-at-microscopy.com} 7, 19 -- Subject: Job opening for Director of the Imaging Technology Group 7, 19 -- Date: Mon, 4 Feb 2008 14:11:22 -0600 7, 19 -- Message-ID: {000d01c8676a$1cd6c290$e87e7e82-at-rollinsitg} 7, 19 -- MIME-Version: 1.0 7, 19 -- Content-Type: text/plain; 7, 19 -- charset="us-ascii" 7, 19 -- Content-Transfer-Encoding: 7bit 7, 19 -- X-Mailer: Microsoft Office Outlook 11 7, 19 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 7, 19 -- Thread-Index: AchnahzBmSMGV2XiT/2U23oeuKbtzg== ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both emer.ryan-at-dit.ie as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: emer.ryan-at-dit.ie Name: Emer Ryan
Organization: CREST DIT
Title-Subject: [Filtered] grease for Sample exchange holder JEOL 8600 Superprobe.
Question: Hello all, I am using a JEOL JXA 8600 superprobe at the moment. Unfortunately, the main microscopist is not available to help me with my query. I am having a problem with sample exchange, as the rod doesn't slide freely in and out of the exchange chamber. I have cleaned/degreased the rod and re-greased again with the lubricant that is there (no brand name available!) but after putting the sample in place, the rod becomes black and sticks on removal. As I am relatively new to microscopy - I would like some advice from someone with a similar instrument regarding the correct grease to use for this situation.
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Email: yongli-at-mcb.harvard.edu Name: Yong Li
Organization: Harvard University
Title-Subject: [Filtered] service for LKB 8800 Ultrotome III
Question: Hi,
There are a LKB 8800 Ultrotome III and its controller LKB 8802A in the lab and they need to be serviced. But as LKB has disappeared, I can't find any company to service on these two machines. Does anyone know a company that does services on the LKB machines?
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Email: jhuisken-at-gmail.com Name: Jan Huisken
Organization: UCSF
Title-Subject: [Filtered] Filter sets for Leica MZ16 F
Question: Hi there,
We are planning on upgrading our Leica Stereoscopes (MZ16F) with new filter sets. It turned out that Leica charges almost twice as much as we would pay at Chroma for just the glass (ca. $1400 vs. $750). Is anybody aware of a company that sells the empty filter holder so that we can mount the filters ourselves?
For a use such as the Jeol SEM's rod, I use Dow Corning High Vacuum Grease. It's a silicon type grease. I do not use Apezon (L or M) vacuum greases for dynamic use, only for static seals. Apiezon greases become sticky, and hard. Silicon grease outgases a bit when fresh, but slides very well. I suppose you may find other brands too (Nye, Monsanto or others). Some people use a dropplet of dif pump oil (DC704/705), but it needs to be cleaned and re-oiled more frequently.
The black traces may be dirt loosen by the freshly greased rod. In case, clean it away with ethanol or aceton, and but a new set of (new) grease. Wip away all grease which could accumulate at the end of the rod. It will be only a dust trap !
I would not use an "unkown" brand of grease, unless you know it has be given by the manufacturer of the intrument for that use.
Hope it helps
J. Faerber IPCMS-GSI (Institut de Physique et Chimie des Matériaux de Strasbourg Groupe Surface et Interfaces) 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
emer.ryan-at-dit.ie a écrit : } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at } http://microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both emer.ryan-at-dit.ie as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: emer.ryan-at-dit.ie } Name: Emer Ryan } } Organization: CREST DIT } } Title-Subject: [Filtered] grease for Sample exchange holder JEOL 8600 } Superprobe. } } Question: Hello all, } I am using a JEOL JXA 8600 superprobe at the moment. Unfortunately, } the main microscopist is not available to help me with my query. } I am having a problem with sample exchange, as the rod doesn't slide } freely in and out of the exchange chamber. } I have cleaned/degreased the rod and re-greased again with the } lubricant that is there (no brand name available!) but after putting } the sample in place, the rod becomes black and sticks on removal. } As I am relatively new to microscopy - I would like some advice from } someone with a similar instrument regarding the correct grease to use } for this situation. } } Thanks in advance. } } } Login Host: 147.252.65.57 } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 8, 11 -- From zaluzec-at-microscopy.com Tue Feb 5 01:24:51 2008 } 8, 11 -- Received: from [10.1.1.21] (dsl-58-6-20-170.wa.westnet.com.au [58.6.20.170]) } 8, 11 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m157OfHB002999 } 8, 11 -- for {microscopy-at-microscopy.com} ; Tue, 5 Feb 2008 01:24:44 -0600 } 8, 11 -- Mime-Version: 1.0 } 8, 11 -- Message-Id: {p06240801c3cdbfa665aa-at-[10.1.1.21]} } 8, 11 -- Date: Tue, 5 Feb 2008 01:24:40 -0600 } 8, 11 -- To: microscopy-at-microscopy.com } 8, 11 -- From: emer.ryan-at-dit.ie (by way of MicroscopyListserver) } 8, 11 -- Subject: viaWWW: grease for Sample exchange holder JEOL 8600 Superprobe } 8, 11 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } ==============================End of - Headers============================== }
==============================Original Headers============================== 10, 30 -- From jacques.faerber-at-ipcms.u-strasbg.fr Tue Feb 5 05:05:59 2008 10, 30 -- Received: from mailhost.u-strasbg.fr (mailhost.u-strasbg.fr [130.79.200.152]) 10, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m15B5w9j015729 10, 30 -- for {microscopy-at-microscopy.com} ; Tue, 5 Feb 2008 05:05:59 -0600 10, 30 -- Received: from ipcms.u-strasbg.fr (ipcms.u-strasbg.fr [130.79.210.2]) 10, 30 -- by mailhost.u-strasbg.fr (8.13.8/jtpda-5.5pre1) with ESMTP id m15B5tSO063782 10, 30 -- for {microscopy-at-microscopy.com} ; Tue, 5 Feb 2008 12:05:55 +0100 (CET) 10, 30 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr [130.79.152.3]) 10, 30 -- by ipcms.u-strasbg.fr (Postfix) with ESMTP id 405767E4038 10, 30 -- for {Microscopy-at-Microscopy.Com} ; Tue, 5 Feb 2008 12:05:06 +0100 (CET) 10, 30 -- Message-ID: {47A842E9.2060007-at-ipcms.u-strasbg.fr} 10, 30 -- Date: Tue, 05 Feb 2008 12:05:13 +0100 10, 30 -- From: "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr} 10, 30 -- User-Agent: Thunderbird 1.5.0.14pre (X11/20071022) 10, 30 -- MIME-Version: 1.0 10, 30 -- To: Microscopy-at-microscopy.com 10, 30 -- Subject: Re: [Microscopy] viaWWW: grease for Sample exchange holder JEOL 8600 10, 30 -- Superprobe 10, 30 -- References: {200802050735.m157Zpfi028274-at-ns.microscopy.com} 10, 30 -- In-Reply-To: {200802050735.m157Zpfi028274-at-ns.microscopy.com} 10, 30 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 10, 30 -- Content-Transfer-Encoding: 8bit 10, 30 -- X-IPCMS-MailScanner: Found to be clean 10, 30 -- X-IPCMS-MailScanner-From: jacques.faerber-at-ipcms.u-strasbg.fr 10, 30 -- X-Greylist: Sender IP whitelisted, not delayed by milter-greylist-3.0 (mailhost.u-strasbg.fr [130.79.200.152]); Tue, 05 Feb 2008 12:05:55 +0100 (CET) 10, 30 -- X-Virus-Scanned: ClamAV 0.88.7/5692/Tue Feb 5 08:21:55 2008 on mr2.u-strasbg.fr 10, 30 -- X-Virus-Status: Clean 10, 30 -- X-Spam-Status: No, score=-0.1 required=5.0 tests=AWL autolearn=disabled 10, 30 -- version=3.1.8 10, 30 -- X-Spam-Checker-Version: SpamAssassin 3.1.8 (2007-02-13) on mr2.u-strasbg.fr ==============================End of - Headers==============================
The College of Microscopy, located in Westmont, IL, is offering the following electron microscopy short courses:
March 25 to 27 - Transmission Electron Microscopy
March 31 to April 4 - Scanning Electron Microscopy
In addition to lectures, these courses emphasize hands-on training using state of the art equipment. For further details and registration information, please follow the link below.
www.collegeofmicroscopy.com
Elaine Schumacher Senior Research Scientist McCrone Associates, Inc. 850 Pasquinelli Drive Westmont, IL 60559-5539 USA 630-887-7100 (tel) 630-887-7417 (fax) E-mail: eschumacher-at-mccrone.com Web Site: www.mccrone.com
==============================Original Headers============================== 11, 27 -- From eschumacher-at-mccrone.com Tue Feb 5 09:15:13 2008 11, 27 -- Received: from pgp.mccrone.com (65-43-97-126.ded.ameritech.net [65.43.97.126]) 11, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m15FFD3G007402 11, 27 -- for {microscopy-at-microscopy.com} ; Tue, 5 Feb 2008 09:15:13 -0600 11, 27 -- Received: from pgp.mccrone.com (localhost [127.0.0.1]) 11, 27 -- by pgp.mccrone.com (Postfix) with ESMTP id 4DF3E1A801A 11, 27 -- for {microscopy-at-microscopy.com} ; Tue, 5 Feb 2008 09:15:14 -0600 (CST) 11, 27 -- Received: from MCCRONEMSG.tmg.mccrone.com ([192.168.101.20]) 11, 27 -- by pgp.mccrone.com (PGP Universal service); 11, 27 -- Tue, 05 Feb 2008 09:15:14 -0600 11, 27 -- X-PGP-Universal: processed 11, 27 -- Content-class: urn:content-classes:message 11, 27 -- MIME-Version: 1.0 11, 27 -- Content-Type: text/plain; 11, 27 -- charset="US-ASCII" 11, 27 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 11, 27 -- Subject: Short Course Announcement: TEM and SEM 11, 27 -- Date: Tue, 5 Feb 2008 09:15:07 -0600 11, 27 -- Message-ID: {2A27CE0EC2A5C748974EA513DFB285A70150DC3D-at-MCCRONEMSG.tmg.mccrone.com} 11, 27 -- X-MS-Has-Attach: 11, 27 -- X-MS-TNEF-Correlator: 11, 27 -- Thread-Topic: Short Course Announcement: TEM and SEM 11, 27 -- Thread-Index: AchoCePUhJHnuV+dQFaDzT0uNKb/Tg== 11, 27 -- From: "Elaine F. Schumacher" {eschumacher-at-mccrone.com} 11, 27 -- To: {microscopy-at-microscopy.com} 11, 27 -- Content-Transfer-Encoding: 8bit 11, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m15FFD3G007402 ==============================End of - Headers==============================
As the topic is still discussed, here are a few sentences on our experience:
For an IGP pump, four years is short. 400 working hours far too short. On our FEI CM12, after 17 years of use, always at 120 keV, about 48 weeks each year and } 40 hours each week, at least 10 or sometimes 15 different users per year, we only have the second IGP now in place, since about 3 or 4 years. We have a TEM with a good vacuum, indeed. Since end 1999, we have a slow-scan CCD - so we do not use film any more. So the TEM is always on and under vacuum, at least 360 days per year. LaB6 filament: changed once in 3 years, only, although frequently used. What we do: we always use LN2, every day. Every evening, we shut down the IGP, for four hours, during the time when the anti-contaminator is warming up. This is part of the software package we have (v.12.5).
This extends the lifetime of the IGP, we were told, by preventing the water, which was trapped at the anti-contaminator, from reaching the IGP. - This apparently helps to keep a good vacuum status, in general.
I have no financial interest in FEI - just a satisfied customer. best regards - and hopefully always a good vacuum, Reinhard
--
---------------------- PD Dr. Reinhard Rachel Universitaet Regensburg Centre for EM - NWF III - -at-Institute for Anatomy Universitaetsstr. 31 D-93053 Regensburg - Germany tel +49 941 943 2837, 1720 fax +49 941 943 2868 mail reinhard.rachel-at-biologie.uni-r.de office: VKL 3.1.29
==============================Original Headers============================== 8, 23 -- From reinhard.rachel-at-biologie.uni-regensburg.de Tue Feb 5 14:47:56 2008 8, 23 -- Received: from rrzmta2.rz.uni-regensburg.de (rrzmta2.rz.uni-regensburg.de [194.94.155.53]) 8, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m15KltU0003015 8, 23 -- for {Microscopy-at-Microscopy.Com} ; Tue, 5 Feb 2008 14:47:56 -0600 8, 23 -- Received: from rrzmta2.rz.uni-regensburg.de (localhost [127.0.0.1]) 8, 23 -- by localhost (Postfix) with SMTP id 818BD55FA3 8, 23 -- for {Microscopy-at-Microscopy.Com} ; Tue, 5 Feb 2008 21:48:03 +0100 (CET) 8, 23 -- Received: from gwsmtp1.uni-regensburg.de (gwsmtp1.rz.uni-regensburg.de [132.199.4.80]) 8, 23 -- by rrzmta2.rz.uni-regensburg.de (Postfix) with ESMTP id 7433D5607B 8, 23 -- for {Microscopy-at-Microscopy.Com} ; Tue, 5 Feb 2008 21:48:03 +0100 (CET) 8, 23 -- Received: from uni-regensburg-MTA by gwsmtp1.uni-regensburg.de 8, 23 -- with Novell_GroupWise; Tue, 05 Feb 2008 21:47:54 +0100 8, 23 -- Message-Id: {47A8D8F3.044C.0054.0-at-biologie.uni-regensburg.de} 8, 23 -- X-Mailer: Novell GroupWise Internet Agent 7.0.2 HP 8, 23 -- Date: Tue, 05 Feb 2008 21:45:23 +0100 8, 23 -- From: "reinhard rachel" {reinhard.rachel-at-biologie.uni-regensburg.de} 8, 23 -- To: {Microscopy-at-Microscopy.Com} 8, 23 -- Subject: ion pump longevity 8, 23 -- Mime-Version: 1.0 8, 23 -- Content-Type: text/plain; charset=US-ASCII 8, 23 -- Content-Disposition: inline 8, 23 -- Content-Transfer-Encoding: 8bit 8, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m15KltU0003015 ==============================End of - Headers==============================
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Email: yuhong.wu-at-solvay.com Name: Yuhong Wu
Title-Subject: [Filtered] Polymer Failure Analysis book
Question: Dear All, I wonder if any of you works on failure analysis of polymers(parts, film/fibers, composites etc.). And if there is any good book/reference on that. I have a few books that have sections of fracture/failure/fractography of polymers, a book named "Compositional and failure analysis of polymers", another book (coming soon) "Fractography - Observing, Measuring and Interpreting Fracture".
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Email: yuhong.wu-at-solvay.com Name: Yuhong Wu
Title-Subject: [Filtered] Polymer Failure Analysis book
Question: Dear All, I wonder if any of you works on failure analysis of polymers(parts, film/fibers, composites etc.). And if there is any good book/reference on that. I have a few books that have sections of fracture/failure/fractography of polymers, a book named "Compositional and failure analysis of polymers", another book (coming soon) "Fractography - Observing, Measuring and Interpreting Fracture".
I have a book that covers all kinds of fiber fracture and damage, polymeric and natural ones. It's an atlas with lots of images and descriptions of the failure mode:
Atlas of fibre fracture and damage to textiles. Second Edition. Hearle J W S, Lomas B & Cooke W D. Woodhead Publishing. ISBN 1 85573 319 6
Best regards,
Petra __________________________________ Dr. Petra Wahlbring Lead Engineer Analytical Test Lab GTC*L Colmar-Berg, Luxembourg e-mail: petra.wahlbring-at-goodyear.com Tel. +352 8199 3725 Fax. +352 8199 5643
- May Contain Confidential and/or Proprietary Information. May not be copied or disseminated without the express written consent of The Goodyear Tire & Rubber Company. -
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Email: yuhong.wu-at-solvay.com Name: Yuhong Wu
Title-Subject: [Filtered] Polymer Failure Analysis book
Question: Dear All, I wonder if any of you works on failure analysis of polymers(parts, film/fibers, composites etc.). And if there is any good book/reference on that. I have a few books that have sections of fracture/failure/fractography of polymers, a book named "Compositional and failure analysis of polymers", another book (coming soon) "Fractography - Observing, Measuring and Interpreting Fracture".
I am trying to prepare stripped and spread red blood cell skeletons for negative stain EM. Briefly it involves isolating red blood cells through centrifugation, hypotonic lysis to remove the hemoglobin, Triton X-100 extraction, and sucrose density gradient centrifugation under high salt conditions. This should yield the basic skeleton of the red blood cell which contains actin, spectrin, and 4.1. I am following the protocol by Shen et al (Ultrastructure of unit fragments of the skeleton of the human erythrocyte membrane. J. Cell. Biol. 99:810-821).
The problem I am having is that the skeletons I am isolating are aggregating on the grids, and I am not sure if it is a problem with my sample preparation protocol, my stain, or the grids. I've tried different support films (holey and continuous, plastic and carbon) and different negative stains (UA and Ammonium molybdate of different concentrations). The grids have been both glow-discharged and non-glow-discharged. Diluting the sample with buffer does not remedy the problem. Is there a chance my prep is aggregating in solution? Some protocols add a little DTT to their suspension so I will next experiment with that. If there is anyone who has experience preparing these skeletons, any pointers would be greatly appreciated. Thank you.
Andrea Nans Graduate Student NYU Med Center
==============================Original Headers============================== 4, 27 -- From andrea.nans-at-gmail.com Wed Feb 6 10:18:43 2008 4, 27 -- Received: from mu-out-0910.google.com (mu-out-0910.google.com [209.85.134.187]) 4, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m16GIgwo001754 4, 27 -- for {Microscopy-at-Microscopy.Com} ; Wed, 6 Feb 2008 10:18:42 -0600 4, 27 -- Received: by mu-out-0910.google.com with SMTP id g7so2529651muf.0 4, 27 -- for {Microscopy-at-Microscopy.Com} ; Wed, 06 Feb 2008 08:18:41 -0800 (PST) 4, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 27 -- d=gmail.com; s=gamma; 4, 27 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 4, 27 -- bh=MwZSAoi9R9uiFpjlI+xlIDpFDz+x4bkjcbdubnCYNds=; 4, 27 -- b=Z6ctCGjMqGcwrjqmFShqSjAMcQJhZWlOcdyNtIT5zQGQq42jWi83vLPGhw8iYlmDy4fZ9nROmmufvH0WMffTSzc7T3vh7u9iQvVoMx4uoS9kxieLyP4G6OfrZBaJ/YYjpd92/Zrj8JIeywtSOuZM0TUCW5Db2n4t5eJS0TcU9Kc= 4, 27 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 4, 27 -- d=gmail.com; s=gamma; 4, 27 -- h=message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 4, 27 -- b=Iom8NtV0LwEIqbCtySHnT6WZOEHEBOpAUxmOmGe/fUaI8Elrpel4An3ZoIu6yMRenFKnJTXsSkDtNKNlLHnz8/TC/o3paD/hgBqaxxLIk5xTSYq2oZ6vVfvdvdhfTJmjzyKvqMtgyop61FINmohLaejdm1uVX6L57J+m1nthCT0= 4, 27 -- Received: by 10.82.159.15 with SMTP id h15mr18250597bue.36.1202314721523; 4, 27 -- Wed, 06 Feb 2008 08:18:41 -0800 (PST) 4, 27 -- Received: by 10.82.179.4 with HTTP; Wed, 6 Feb 2008 08:18:41 -0800 (PST) 4, 27 -- Message-ID: {f4f4e6200802060818o54a61c4w6b122e870c042861-at-mail.gmail.com} 4, 27 -- Date: Wed, 6 Feb 2008 11:18:41 -0500 4, 27 -- From: "Andrea Nans" {andrea.nans-at-gmail.com} 4, 27 -- To: Microscopy-at-Microscopy.Com 4, 27 -- Subject: TEM Sample Prep- Red Cell Skeleton Spread 4, 27 -- MIME-Version: 1.0 4, 27 -- Content-Type: text/plain; charset=ISO-8859-1 4, 27 -- Content-Transfer-Encoding: 7bit 4, 27 -- Content-Disposition: inline ==============================End of - Headers==============================
I am thinking about buying a Wacom Cintiq monitor/tablet so that I can trace objects during on the screen for Photoshop operations (e.g., area measurements using the Fovea Pro toolkit). I would welcome comments from those using this approach about its ease and success rate. The 6 x 10 inch workspace monitor is ~$1000 while the 17 x 10 is ~$2000. Any thoughts on the size of the tablet or the DTF vs. Cintiq models would also be useful. Thanks, Tom
Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
Lothar Engel et al., An Atlas of Polymer Damage - Surface Examination by Scanning Electron Microscope, Prentice-Hall, New Jersey (1981) has been useful to me. It is not comprehensive but helped in understanding the morphology of polyolefin failure as seen using optical, SEM and TEM.
Regards,
Disclaimer: The comments and opinions given above are those of the author alone and do not represent any position of ExxonMobil Chemical Company.
Gary M. Brown Microscopy Specialist ExxonMobil Chemical Company Baytown Technology & Engineering - West 5200 Bayway Drive Baytown, Texas 77520-2101 phone: 281 834 2387 fax: 281 834 2395 e-mail: Gary.M.Brown-at-ExxonMobil.com
"Happiness equals reality minus expectations" Tom Magliozzi
yuhong.wu-at-solv ay.com To gary.m.brown-at-exxonmobil.com 02/05/08 11:25 cc PM Subject [Microscopy] viaWWW: Polymer Failure Please respond Analysis book to yuhong.wu-at-solv ay.com
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Email: yuhong.wu-at-solvay.com Name: Yuhong Wu
Title-Subject: [Filtered] Polymer Failure Analysis book
Question: Dear All, I wonder if any of you works on failure analysis of polymers(parts, film/fibers, composites etc.). And if there is any good book/reference on that. I have a few books that have sections of fracture/failure/fractography of polymers, a book named "Compositional and failure analysis of polymers", another book (coming soon) "Fractography - Observing, Measuring and Interpreting Fracture".
This Question was submitted to Ask-A-Microscopist by (mcaruso2-at-uiuc.edu) from http://www.microscopy.org/Ask-A-Microscopist/Ask-A-Microscopist.html on Wednesday, February 6, 2008 at 17:31:31 Remember to consider the Grade/Age of the student when considering the Question --------------------------------------------------------------------------- Please reply to both mcaruso2-at-uiuc.edu as well as to the Microscopy Listserver ---------------------------------------------------------------------------
Email: mcaruso2-at-uiuc.edu Name: Mary M Caruso
Organization: University of Illinois at Urbana-Champaign
Education: Graduate College
Location: Urbana, IL, USA
Title: Stain for imaging amines in epoxy resin
Question: Do you know of a stain for electron microscopy that is functional group specific? We would like to add a stain or dye that would tag amines in an epoxy matrix to show that some of them have not completely reacted in the 3D polymer network. I tried ninhydrin as a stain and can see the purple color appear on the surface of the resin but imaging these were more difficult. Thank you for your help. ~Mary
We used MetaMorph back at UCL and I bought a small $100 Wacom Graphire version 2 A5 Tablet for image editing and tracing regions. The pen with this set was superior to the latest (cheaper) Graphire v4 as the buttons were located further down the pen as a single rocker and using the buttons was far easier - which really affects how nice it is to use the tablet. The old style pen looked more like the far more expensive Intuos 3 style. We didn't need any larger than the little A5 pad for drawing around images of cells/tissue on 22 inch CRT screen (any photos/negatives were scanned into the PC rather than placed under the tablet to trace over). I would be interested to hear from any who found the larger A4/A3 Wacon Intuos useful for tracing (we don't do 'graphics' with it at work, it's always just tracing, although my kids use a Graphire 2 with Corel Painter X at home).
Initially the works Wacom Graphire 2 tablet was hardly used and I disliked it as the pen (and Wacom mouse) was so poor for basic Windows chores. However when I had hundreds of cells to trace the Wacom Graphire 2 pad came into it's own and it really helped tracing speed and accuracy (I just wouldn't use a mouse to trace now if the pad was available). That said you need the PC optical mouse as well to use menus etc.., they work happily with the pen, you just drop the pen into it's holder and take over the curser with the optical mouse when you have finished tracing (the pen providing the double click to complete the trace).
When an unknown user lost the Graphire Pen (it looks like a normal pen and probably got thrown away) we bought a cheap Graphire 4 pad - but I was really unhappy with the pen button locations and ended up paying £30 for a replacement Graphire 2 pen and used that pad instead (may just be habit). The graphire 4 was half the price of the Graphire 2 (which did include a rather naf mouse). So I would be tempted to go upmarket beyond the $80 Graphire 4, and go for something like an Intuos - and we found the smaller A5 size perfect to fit on the desktop with a MS Intellimouse mouse.
I think an A4 tablet might have taken up too much room, but we bought the little A5 Graphire 2 on a tight budget to try it out and never felt the need to upgrade. I think the Cintiq may be going over the top just to trace objects (and you can use the Intuos pen with the Cintiq if you upgrade later) - but if that's all someone ever does in the lab, why not. Personally I like using my PC monitor with a space saving A5 Wacom pad (that’s the same size as the mouse pad). Our old Magiscan image analyzer back in the late 1980s had a light pen for drawing on the CRT screen (push on the glass screen to 'mouse click') for image editing and that worked really well [largely as the Magiscan image editing software was so good], so an expensive Cintiq should be very successful as well. I did look into light pens but they are expensive and aren't so software/LCD screen friendly as tablets.
Enlarging the object [Zoom/magnify] onscreen really helped with accurate tracing, and metaMorph has a useful back untrace function if you slip too far off target. Some software just zooms in the area where you tracing, which can be useful (I think ImagePro Plus v4/5 [Bio-rad LaserPix] did that, but I can't remember exactly).
So the very cheap A5 wacom tablet was a success for us for occasional tracing of microscope images - nearly always for tracing around cells from wide field phase contrast [transmission] optical microscope images, eg. For cell extention/retraction studies during wound healing. Have you tried a cheaper Wacom Intuos 3 tablet and are wanting to upgrade for some reason ?
Regards
Keith
-------------------------------------------------------------------------- Dr keith J. Morris Molecular Cytogenetics and Microscopy Core Laboratory 00/069 and 00/070 The Wellcome Trust Centre for Human Genetics Roosevelt Drive Oxford OX3 7BN United Kingdom Telephone: +44 (0)1865 287568 Email: kjmorris-at-well.ox.ac.uk Web-pages: http://www.well.ox.ac.uk/cytogenetics/
-----Original Message----- X-from: PhillipsT-at-missouri.edu [mailto:PhillipsT-at-missouri.edu] Sent: 06 February 2008 16:36 To: kjmorris-at-well.ox.ac.uk
I am thinking about buying a Wacom Cintiq monitor/tablet so that I can trace objects during on the screen for Photoshop operations (e.g., area measurements using the Fovea Pro toolkit). I would welcome comments from those using this approach about its ease and success rate. The 6 x 10 inch workspace monitor is ~$1000 while the 17 x 10 is ~$2000. Any thoughts on the size of the tablet or the DTF vs. Cintiq models would also be useful. Thanks, Tom
Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
I'm looking for a control box for my Ultracut E ultramicrotome. If anyone has one they would like to sell please contact me. Thanks.
Tom Bargar University of Nebraska Medical Center Core Electron Microscopy Research Facility 986395 Nebraska Medical Center Omaha, NE 68198-6395 402-559-7347 tbargar-at-unmc.edu
==============================Original Headers============================== 4, 20 -- From tbargar-at-unmc.edu Thu Feb 7 09:35:00 2008 4, 20 -- Received: from zixvpm02.unmc.edu (zixvpm02.unmc.edu [192.198.54.127]) 4, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m17FZ0fH024002 4, 20 -- for {Microscopy-at-MSA.Microscopy.com} ; Thu, 7 Feb 2008 09:35:00 -0600 4, 20 -- Received: from zixvpm02.unmc.edu (ZixVPM [127.0.0.1]) 4, 20 -- by Outbound.unmc.edu (Proprietary) with ESMTP id DFB89A0062 4, 20 -- for {Microscopy-at-MSA.Microscopy.com} ; Thu, 7 Feb 2008 09:34:57 -0600 (CST) 4, 20 -- Received: from unmcnotes01.unmc.edu (host-137-197-103-35.unmc.edu [137.197.103.35]) 4, 20 -- by zixvpm02.unmc.edu (Proprietary) with ESMTP id 6B64739804A 4, 20 -- for {Microscopy-at-MSA.Microscopy.com} ; Thu, 7 Feb 2008 09:34:56 -0600 (CST) 4, 20 -- Subject: looking for an Ultracut E control box 4, 20 -- To: Microscopy-at-MSA.Microscopy.com 4, 20 -- X-Mailer: Lotus Notes Release 7.0.1 January 17, 2006 4, 20 -- Message-ID: {OF1DB1D819.63B728E6-ON862573E8.00555EA9-862573E8.0055982B-at-unmc.edu} 4, 20 -- From: Tom W Bargar {tbargar-at-unmc.edu} 4, 20 -- Date: Thu, 7 Feb 2008 09:34:55 -0600 4, 20 -- X-MIMETrack: Serialize by Router on UNMCNOTES01.UNMC.EDU/Servers/UNEBR at 02/07/2008 09:34:56 4, 20 -- AM 4, 20 -- MIME-Version: 1.0 4, 20 -- Content-type: text/plain; charset=US-ASCII ==============================End of - Headers==============================
On Feb 7, 2008, at 3:40 AM, kjmorris-at-well.ox.ac.uk wrote:
} I have not used the Cintiq (I don't have that kind of money), but I have been using the Wacom Intuos for years now. For tracing objects it is fantastic. For use with photoshop it is great. I have found an added benefit. Switching between the 'pen' and the mouse has alleviated some of my carpal tunnel problems. The improvement has been great enough I got one for home also. Another advantage for me is that I now have a mouse with 5 buttons that can be individually programed for different applications. I have gotten very used to this convenience. It is hard to go back to the one button Mac mouse :-) I find the Wacom mouse (I have only used it on the larger tablets) to be very usable.
David } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I am thinking about buying a Wacom Cintiq monitor/tablet so that I can } trace objects during on the screen for Photoshop operations (e.g., } area } measurements using the Fovea Pro toolkit). I would welcome comments } from those using this approach about its ease and success rate. The } 6 x } 10 inch workspace monitor is ~$1000 while the 17 x 10 is ~$2000. Any } thoughts on the size of the tablet or the DTF vs. Cintiq models would } also be useful. Thanks, Tom } } Thomas E. Phillips, Ph.D } Professor of Biological Sciences } Director, Molecular Cytology Core } 2 Tucker Hall } University of Missouri } Columbia, MO 65211-7400 } 573-882-4712 (office) } 573-882-0123 (fax) } phillipst-at-missouri.edu } } http://www.biology.missouri.edu/faculty/phillips.html } http://www.biotech.missouri.edu/mcc/ } } } } } ==============================Original } Headers============================== } 6, 23 -- From PhillipsT-at-missouri.edu Wed Feb 6 10:23:14 2008 } 6, 23 -- Received: from um-nsmtpout1.um.umsystem.edu } (um-nsmtpout1.um.umsystem.edu [209.106.228.53]) } 6, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } m16GNEgD004656 } 6, 23 -- for {Microscopy-at-msa.microscopy.com} ; Wed, 6 Feb 2008 } 10:23:14 -0600 } 6, 23 -- Received: from UM-XMAIL06.um.umsystem.edu } ([209.106.228.32]) by } um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); } 6, 23 -- Wed, 6 Feb 2008 10:23:13 -0600 } 6, 23 -- x-mimeole: Produced By Microsoft Exchange V6.5 } 6, 23 -- Content-class: urn:content-classes:message } 6, 23 -- MIME-Version: 1.0 } 6, 23 -- Content-Type: text/plain; } 6, 23 -- charset="us-ascii" } 6, 23 -- Subject: Wacom tablets/touch-screen monitors } 6, 23 -- Date: Wed, 6 Feb 2008 10:23:11 -0600 } 6, 23 -- Message-ID: } {0510DC719E56F64BB2AD84EE64CE6BAD03726878-at-UM-XMAIL06.um.umsystem.edu} } 6, 23 -- X-MS-Has-Attach: } 6, 23 -- X-MS-TNEF-Correlator: } 6, 23 -- Thread-Topic: Wacom tablets/touch-screen monitors } 6, 23 -- Thread-Index: Acho3JDHWj+Txz4CR9u0MVm9e7Fvlw== } 6, 23 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu} } 6, 23 -- To: {Microscopy-at-msa.microscopy.com} } 6, 23 -- X-OriginalArrivalTime: 06 Feb 2008 16:23:13.0653 (UTC) } FILETIME=[924AD250:01C868DC] } 6, 23 -- Content-Transfer-Encoding: 8bit } 6, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id m16GNEgD004656 } ==============================End of - } Headers============================== } } } } ==============================Original } Headers============================== } 25, 23 -- From kjmorris-at-well.ox.ac.uk Thu Feb 7 04:36:05 2008 } 25, 23 -- Received: from morse.well.ox.ac.uk (morse.well.ox.ac.uk } [129.67.44.2]) } 25, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id m17Aa4Xs031815 } 25, 23 -- for {Microscopy-at-Microscopy.Com} ; Thu, 7 Feb 2008 04:36:05 } -0600 } 25, 23 -- Received: from dhcp079.well.ox.ac.uk ([129.67.44.178] } helo=CytoWhizz) } 25, 23 -- by morse.well.ox.ac.uk with esmtp (Exim 4.52) } 25, 23 -- id 1JN46u-0000lC-E5 } 25, 23 -- for Microscopy-at-Microscopy.Com; Thu, 07 Feb 2008 10:36:04 } +0000 } 25, 23 -- From: "Keith Morris" {kjmorris-at-well.ox.ac.uk} } 25, 23 -- To: {Microscopy-at-Microscopy.Com} } 25, 23 -- References: {200802061635.m16GZtDL025918-at-ns.microscopy.com} } 25, 23 -- Subject: RE: [Microscopy] Wacom tablets/touch-screen } monitors } 25, 23 -- Date: Thu, 7 Feb 2008 10:36:24 -0000 } 25, 23 -- Message-ID: {000801c86975$49d64b50$b22c4381-at-CytoWhizz} } 25, 23 -- MIME-Version: 1.0 } 25, 23 -- Content-Type: text/plain; } 25, 23 -- charset="iso-8859-1" } 25, 23 -- X-Mailer: Microsoft Office Outlook 11 } 25, 23 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 } 25, 23 -- Thread-Index: Acho3llI7zRt/XwiRjyXX6VRewlknAAiwREg } 25, 23 -- In-Reply-To: {200802061635.m16GZtDL025918-at-ns.microscopy.com} } 25, 23 -- Content-Transfer-Encoding: 8bit } 25, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id m17Aa4Xs031815 } ==============================End of - } Headers============================== }
==============================Original Headers============================== 5, 22 -- From Elliott-at-arizona.edu Thu Feb 7 09:54:53 2008 5, 22 -- Received: from smtpgate.email.arizona.edu (frodo.email.Arizona.EDU [128.196.133.168]) 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m17FsrZh004883 5, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 7 Feb 2008 09:54:53 -0600 5, 22 -- Received: from frodos_amavis (amavis7.email.arizona.edu [10.0.0.235]) 5, 22 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id 97DC3290775 5, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 7 Feb 2008 08:54:52 -0700 (MST) 5, 22 -- Received: from [192.168.0.30] (doctorelliott.us [70.57.226.104]) 5, 22 -- by smtpgate.email.arizona.edu (Postfix) with ESMTP id B57F428C5A5 5, 22 -- for {Microscopy-at-MSA.Microscopy.Com} ; Thu, 7 Feb 2008 08:54:44 -0700 (MST) 5, 22 -- Message-Id: {BCEA8D2D-9479-4951-B862-397D3CC039B0-at-arizona.edu} 5, 22 -- From: David Elliott {Elliott-at-arizona.edu} 5, 22 -- To: Microscopy ListServer {Microscopy-at-MSA.Microscopy.Com} 5, 22 -- In-Reply-To: {200802071040.m17Aeku2004223-at-ns.microscopy.com} 5, 22 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 5, 22 -- Content-Transfer-Encoding: 7bit 5, 22 -- Mime-Version: 1.0 (Apple Message framework v915) 5, 22 -- Subject: Re: [Microscopy] RE: Wacom tablets/touch-screen monitors 5, 22 -- Date: Thu, 7 Feb 2008 08:54:31 -0700 5, 22 -- References: {200802071040.m17Aeku2004223-at-ns.microscopy.com} 5, 22 -- X-Mailer: Apple Mail (2.915) 5, 22 -- X-Virus-Scanned: amavisd-new at email.arizona.edu ==============================End of - Headers==============================
Memorial University of Newfoundland, located in St. John's, is pleased to announce a short course on "Mineral Liberation Analysis" this spring, May 12-14, as well as an advanced course May 15-16. The courses are aimed at minerals industry professionals and attendance is limited. More information can be found in the announcement broshure: http://www.mun.ca/creait/maf/MUN_MLA_Short_Course_2008_2.pdf
... and at our Micro-analysis Facility's website: http://www.mun.ca/creait/maf/index.php and http://www.mun.ca/creait/maf/mla.php
Briefly, "Mineral Liberation Analysis" (MLA) refers primarily to economic minerals and quantifying the degree to which they're liberated (or locked) relative to other non-economic minerals. However, these specific mineral relationships are only a subset of mineral associations for which the MLA is also quite capable of quantifying. Other applications include point-counting to rare-phase searches. In the past, MLA instrumentation has been employed primarily at minerals' industry research locations worldwide; however, has recently found its way into university research in the last several years.
"Mineral Liberation Analysis" is a software solution that automates the SEM's BEI and stage, as well as integrates high-speed x-ray spectral acquisition (e.g., dual Bruker SDD x-ray detectors). The software was developed at the University of Queensland's Julius Kruttschnitt Mineral Research Centre (JKMRC), which is now the technology transfer company JKTech Pty Ltd: http://www.jktech.com.au/Products_Services/MLA/index.htm
Genuinely, Michael Shaffer :o) SEM-MLA Research Coordinator INCO Innovation Ctr Memorial University 230 Elizabeth Avenue St. John's Newfoundland A1C5S7 (709) 737-6799 (Ofc & Msg) (709) 737-6790 (SEM Lab) (709) 737-6193 (FAX) http://www.mun.ca/creait/maf/
==============================Original Headers============================== 6, 27 -- From michael-at-shaffer.net Thu Feb 7 10:06:33 2008 6, 27 -- Received: from smtprelay.hostedemail.com (smtprelay0062.hostedemail.com [216.40.44.62]) 6, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m17G6WQX017640 6, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 7 Feb 2008 10:06:33 -0600 6, 27 -- Received: from emd2-omf02.hostedemail.com (ff-bigip1 [10.5.19.254]) 6, 27 -- by smtprelay01.hostedemail.com (Postfix) with ESMTP id 907F8FB9EF 6, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 7 Feb 2008 16:06:31 +0000 (UTC) 6, 27 -- X-SpamScore: 50 6, 27 -- X-Spamcatcher-Summary: 50,0,0,418fa7ea1b279db1,adfbb84ca675e7e0,michael-at-shaffer.net,-,RULES_HIT:10:355:379:539:541:542:967:973:988:989:1042:1155:1156:1160:1260:1277:1311:1313:1314:1345:1391:1437:1515:1516:1518:1534:1541:1593:1594:1711:1730:1747:1766:1792:2075:2078:2198:2199:2377:2378:2380:2393:2525:2553:2567:2682:2685:2857:2859:2892:2933:2937:2939:2942:2945:2947:2951:2954:3022:3353:3636:3657:3865:3866:3867:3868:3869:3870:3871:3874:3934:3936:3938:3941:3944:4250:4321:4470:5007:6119: 6, 27 -- 6261:7679,0,RBL:none,CacheIP:none,Bayesian:0.5,0.5,0.5,Netcheck:none,DomainCache:0,MSF:not bulk,SPF:,MSBL:none,DNSBL:none 6, 27 -- X-Spamcatcher-Explanation: 6, 27 -- Received: from roamingwolf (roaming-wolf.creait.mun.ca [134.153.130.141]) 6, 27 -- (Authenticated sender: michael-at-shaffer.net) 6, 27 -- by emd2-omf02.hostedemail.com (Postfix) with ESMTP 6, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 7 Feb 2008 16:06:31 +0000 (UTC) 6, 27 -- From: "michael shaffer" {michael-at-shaffer.net} 6, 27 -- To: "MSA Microscopy list" {Microscopy-at-microscopy.com} 6, 27 -- Subject: Short course announcement: SEM-platform image analysis 6, 27 -- Date: Thu, 7 Feb 2008 12:43:59 -0330 6, 27 -- Message-ID: {000a01c869a4$745b9090$8d829986-at-CREAIT.MUN.CA} 6, 27 -- MIME-Version: 1.0 6, 27 -- Content-Type: text/plain; 6, 27 -- charset="US-ASCII" 6, 27 -- Content-Transfer-Encoding: 7bit 6, 27 -- X-Mailer: Microsoft Office Outlook 11 6, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 6, 27 -- Thread-Index: AchppHJoVzH9ijfXSOaB7eEctjC6KQ== ==============================End of - Headers==============================
Keith, thanks for a thorough and informative post! And Tom, have you actually been using a tablet already and now are considering an upgrade to Cintiq? Or you haven't use either yet?
Anyway, I have just one comment to add now: that our hand (even my clumsy one) seems to adjust very easily to any scaling when you trace with a Wacom pen on your tablet while looking at the screen. Seriously, I remember I was surprised at first. The hand learns immediately and has no trouble following the outline, even if the magnification on the screen is much higher. That means that bigger tablet may not be better. Think about how exactly you will be using it - setting on your desk in front of the monitor? or on your lap? on your knee sitting with your legs crossed? We found Wacom's second small size to be optimal. Bigger tablet can feel really bulky and harder to balance; besides, there are those buttons on the tablet's sides, and you won't be able to reach them as easy.
Vlad ________________________________________________ Vlad Speransky, Staff Scientist Supramolecular Structure and Function Resource National Institute of Biomedical Imaging and Bioengineering, NIH 13 South Dr, Rm. 3N17 MSC 5766 Bethesda, MD 20892 301 496-3989 vladislav_speransky-at-nih.gov
==============================Original Headers============================== 5, 22 -- From vladislav_speransky-at-nih.gov Thu Feb 7 11:57:56 2008 5, 22 -- Received: from nihrelayxway3.hub.nih.gov (nihrelayxway3.hub.nih.gov [128.231.90.108]) 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m17HvtHn002067 5, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 7 Feb 2008 11:57:55 -0600 5, 22 -- X-IronPortListener: NIH_Relay 5, 22 -- X-SBRS: None 5, 22 -- X-IronPort-AV: E=Sophos;i="4.25,316,1199682000"; 5, 22 -- d="scan'208";a="300074426" 5, 22 -- Received: from unknown (HELO helix.nih.gov) ([128.231.90.83]) 5, 22 -- by nihrelayxway3.hub.nih.gov with ESMTP; 07 Feb 2008 12:57:56 -0500 5, 22 -- Received: from [128.231.217.77] (db477.niaid.nih.gov [128.231.217.77]) 5, 22 -- by helix.nih.gov (8.13.6/8.13.6/2SCANNER) with ESMTP id m17HvtTV21648775 5, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 7 Feb 2008 12:57:55 -0500 (EST) 5, 22 -- Mime-Version: 1.0 (Apple Message framework v753) 5, 22 -- Content-Transfer-Encoding: 7bit 5, 22 -- Message-Id: {4CE9FF05-2EF1-42AE-B116-D26BA3D66A52-at-nih.gov} 5, 22 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 5, 22 -- To: Microscopy-at-microscopy.com 5, 22 -- From: Vlad Speransky {vladislav_speransky-at-nih.gov} 5, 22 -- Subject: [Microscopy] RE: Wacom tablets/touch-screen monitors 5, 22 -- Date: Thu, 7 Feb 2008 12:57:37 -0500 5, 22 -- X-Mailer: Apple Mail (2.753) ==============================End of - Headers==============================
I have not complained before about course presentations but I feel Michaels short course announcement goes beyond a course announcement and becomes a sales presentation on a product.
Those of us who earn our crust through microscopy and selling a service take great care not to step into a sales presentation situation; Michael I believe has overstepped the mark?
Steve Chapman FRMS Protrain for EM training & consultancy world wide www.emcourses.com Tel +44 1280 816512 Fax +44 1280 814007 Mobile +44 7711 606967
==============================Original Headers============================== 6, 28 -- From protrain-at-emcourses.com Thu Feb 7 12:02:24 2008 6, 28 -- Received: from smtp01.dial-up.net (smtp01.dial-up.net [196.26.208.170]) 6, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m17I2MV5006102 6, 28 -- for {microscopy-at-microscopy.com} ; Thu, 7 Feb 2008 12:02:23 -0600 6, 28 -- Received: from 5ac8bf5d.bb.sky.com ([90.200.191.93]:3460 helo=HP6220) 6, 28 -- by smtp01.dial-up.net with esmtpa (Exim 4.68 #0) 6, 28 -- (envelope-from {protrain-at-emcourses.com} ) 6, 28 -- id 1JNB4l-0007D2-Tp by authid {f1f952b73f4f9e0d4f7dbb81b71a957d} with fixed_login 6, 28 -- for {microscopy-at-microscopy.com} ; Thu, 07 Feb 2008 20:02:21 +0200 6, 28 -- Message-ID: {001701c869b3$852e5dd0$0200a8c0-at-HP6220} 6, 28 -- Reply-To: "Steve Chapman" {protrain-at-emcourses.com} 6, 28 -- From: "Steve Chapman" {protrain-at-emcourses.com} 6, 28 -- To: "American Society" {microscopy-at-microscopy.com} 6, 28 -- Subject: Michaels Short Course announcement 6, 28 -- Date: Thu, 7 Feb 2008 18:01:17 -0000 6, 28 -- Organization: Protrain 6, 28 -- MIME-Version: 1.0 6, 28 -- Content-Type: text/plain; 6, 28 -- format=flowed; 6, 28 -- charset="Windows-1252"; 6, 28 -- reply-type=original 6, 28 -- Content-Transfer-Encoding: 7bit 6, 28 -- X-Priority: 3 6, 28 -- X-MSMail-Priority: Normal 6, 28 -- X-Mailer: Microsoft Outlook Express 6.00.2900.3138 6, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 6, 28 -- X-Scan-Signature: c3253b1522b319b8bde41fef99cb3562{17}} 6, 28 -- X-Trace: smtp01.dial-up.net 1JNB4l-0007D2-Tp cc945f1d1d9f618f8dfc8a00dac4f84b ==============================End of - Headers==============================
I haven't used any type of tablet before. I must admit that reading about them in some of the posts makes me wonder why I haven't. It is a less expensive option. But some of the other posts are clearly extolling the Cintiq which allows direct tracing on a near horizontal monitor. I have used a mouse to trace 1000's of objects and I am fairly adept but I know that I would have to be 10x faster tracing an object on a photograph or, in this case, the monitor. I was mostly looking to hear that this was as good as it seems on paper and it sounds like it should be. I am definitely going to bite the bullet and buy either the small or medium size monitor. I found the medium size one for 1900 and probably will go with that. I will eventually post a comment about my results once I get the new toy. I appreciate all the helpful comments. Tom
Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
-----Original Message----- X-from: vladislav_speransky-at-nih.gov [mailto:vladislav_speransky-at-nih.gov] Sent: Thursday, February 07, 2008 11:59 AM To: Phillips, Thomas E.
Hello Tom and Keith,
Keith, thanks for a thorough and informative post! And Tom, have you actually been using a tablet already and now are considering an upgrade to Cintiq? Or you haven't use either yet?
Anyway, I have just one comment to add now: that our hand (even my clumsy one) seems to adjust very easily to any scaling when you trace with a Wacom pen on your tablet while looking at the screen. Seriously, I remember I was surprised at first. The hand learns immediately and has no trouble following the outline, even if the magnification on the screen is much higher. That means that bigger tablet may not be better. Think about how exactly you will be using it - setting on your desk in front of the monitor? or on your lap? on your knee sitting with your legs crossed? We found Wacom's second small size to be optimal. Bigger tablet can feel really bulky and harder to balance; besides, there are those buttons on the tablet's sides, and you won't be able to reach them as easy.
Vlad ________________________________________________ Vlad Speransky, Staff Scientist Supramolecular Structure and Function Resource National Institute of Biomedical Imaging and Bioengineering, NIH 13 South Dr, Rm. 3N17 MSC 5766 Bethesda, MD 20892 301 496-3989 vladislav_speransky-at-nih.gov
==============================Original Headers============================== 5, 22 -- From vladislav_speransky-at-nih.gov Thu Feb 7 11:57:56 2008 5, 22 -- Received: from nihrelayxway3.hub.nih.gov (nihrelayxway3.hub.nih.gov [128.231.90.108]) 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m17HvtHn002067 5, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 7 Feb 2008 11:57:55 -0600 5, 22 -- X-IronPortListener: NIH_Relay 5, 22 -- X-SBRS: None 5, 22 -- X-IronPort-AV: E=Sophos;i="4.25,316,1199682000"; 5, 22 -- d="scan'208";a="300074426" 5, 22 -- Received: from unknown (HELO helix.nih.gov) ([128.231.90.83]) 5, 22 -- by nihrelayxway3.hub.nih.gov with ESMTP; 07 Feb 2008 12:57:56 -0500 5, 22 -- Received: from [128.231.217.77] (db477.niaid.nih.gov [128.231.217.77]) 5, 22 -- by helix.nih.gov (8.13.6/8.13.6/2SCANNER) with ESMTP id m17HvtTV21648775 5, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 7 Feb 2008 12:57:55 -0500 (EST) 5, 22 -- Mime-Version: 1.0 (Apple Message framework v753) 5, 22 -- Content-Transfer-Encoding: 7bit 5, 22 -- Message-Id: {4CE9FF05-2EF1-42AE-B116-D26BA3D66A52-at-nih.gov} 5, 22 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 5, 22 -- To: Microscopy-at-microscopy.com 5, 22 -- From: Vlad Speransky {vladislav_speransky-at-nih.gov} 5, 22 -- Subject: [Microscopy] RE: Wacom tablets/touch-screen monitors 5, 22 -- Date: Thu, 7 Feb 2008 12:57:37 -0500 5, 22 -- X-Mailer: Apple Mail (2.753) ==============================End of - Headers==============================
==============================Original Headers============================== 16, 26 -- From PhillipsT-at-missouri.edu Thu Feb 7 13:39:10 2008 16, 26 -- Received: from um-tsmtpout1.um.umsystem.edu (um-tsmtpout1.um.umsystem.edu [209.106.228.23]) 16, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m17JdAOi030895 16, 26 -- for {Microscopy-at-msa.microscopy.com} ; Thu, 7 Feb 2008 13:39:10 -0600 16, 26 -- Received: from UM-XMAIL06.um.umsystem.edu ([209.106.228.32]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); 16, 26 -- Thu, 7 Feb 2008 13:39:09 -0600 16, 26 -- x-mimeole: Produced By Microsoft Exchange V6.5 16, 26 -- Content-class: urn:content-classes:message 16, 26 -- MIME-Version: 1.0 16, 26 -- Content-Type: text/plain; 16, 26 -- charset="us-ascii" 16, 26 -- Subject: RE: [Microscopy] RE: Wacom tablets/touch-screen monitors 16, 26 -- Date: Thu, 7 Feb 2008 13:39:07 -0600 16, 26 -- Message-ID: {0510DC719E56F64BB2AD84EE64CE6BAD038C96E1-at-UM-XMAIL06.um.umsystem.edu} 16, 26 -- In-Reply-To: {200802071759.m17Hx6SO003334-at-ns.microscopy.com} 16, 26 -- X-MS-Has-Attach: 16, 26 -- X-MS-TNEF-Correlator: 16, 26 -- Thread-Topic: [Microscopy] RE: Wacom tablets/touch-screen monitors 16, 26 -- Thread-Index: AchpsyjSCQHx2QGeTue5OKjW/VH0WwADVTDg 16, 26 -- References: {200802071759.m17Hx6SO003334-at-ns.microscopy.com} 16, 26 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu} 16, 26 -- To: {vladislav_speransky-at-nih.gov} 16, 26 -- Cc: {Microscopy-at-msa.microscopy.com} 16, 26 -- X-OriginalArrivalTime: 07 Feb 2008 19:39:09.0951 (UTC) FILETIME=[1C0148F0:01C869C1] 16, 26 -- Content-Transfer-Encoding: 8bit 16, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m17JdAOi030895 ==============================End of - Headers==============================
We are attempting to repair a Wild M20 light microscope for a colleague. The fine focus knob is no longer independently moveable but is "fused" to the coarse focus. I believe the gearing mechanism and associated metal parts need to be cleaned of polymerized lubricant. I have disassembled the scope to gain access to the gearing but cannot figure out how to remove the focus knobs to expose the gearing. Does anyone know how this might be done? Thank you. -- +++++++++++++++++++++++++++++++++++++++++++++++++++++++
John J. Bozzola, Ph.D., Director Integrated Microscopy & Graphics Expertise (IMAGE) Southern Illinois University 750 Communications Drive - MC 4402 Carbondale, IL 62901 Telephone: 618-453-3730
No I don't present my last book, I just need your help to solve a mysterious peak in EDX ;-) Here is the story: A colleague was analysing a piece of zeolite in SEM with EDX (Tescan SEM, Oxford Instr. EDX). The specimen was sputter-coated with carbon and the HT was 20 keV (W filament). I told her to try stick with a deadtime of about 20% and adjusted the parameters accordingly. At that moment we had the nice Al peak (1,49 keV), Si peak (1,74 keV) and a smaller but very clear Ca peak (3,69 keV). Happyness at its best. Then I left her to have a cup of coffee...eer I mean to study a very difficult case and when I was back she said she found some mercury in the sample! (which is absolutely unexpected even as contaminant). She had increased the spot size and had a deadtime of more than 70%!! And indeed she had a very tiny Hg peak at 2,19 keV! All others parameters being the same, when I decreased the spot size the Hg peak disappeared. This is obviously an artifact, but after reading the book from Goldstein, Newbury at al. I cannot find to which artifact belongs that Hg peak. Any thought about it?
Best regards,
Stephane
PS: I will summarize the answers I got from my question about the IGP pump replacement soon
____________________________________________________________________________________ Never miss a thing. Make Yahoo your home page. http://www.yahoo.com/r/hs
==============================Original Headers============================== 7, 19 -- From nizets2-at-yahoo.com Fri Feb 8 02:15:33 2008 7, 19 -- Received: from web37410.mail.mud.yahoo.com (web37410.mail.mud.yahoo.com [209.191.91.142]) 7, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m188FWUP013082 7, 19 -- for {microscopy-at-microscopy.com} ; Fri, 8 Feb 2008 02:15:32 -0600 7, 19 -- Received: (qmail 83548 invoked by uid 60001); 8 Feb 2008 08:15:32 -0000 7, 19 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 7, 19 -- s=s1024; d=yahoo.com; 7, 19 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Message-ID; 7, 19 -- b=ZuHUvPzVvVcxTFeG/DZUKSUZzfXJBzbC2EligDPZuZSzGDp4Mb0sUIRO0Lh4loLr2QhuZxiokdnkAiLfSdv1Fel1wI3WHvn7WcOSPKBqJwjlPJfP3lelZHP6Engxx8ID1+6J9jh1paTTX1pQyPVYYUg/6OoA1RZjC+mYUb3WABM=; 7, 19 -- X-YMail-OSG: rwxGQEEVM1kny9k7VgIi2iKvuAvYQTepPc_QTIfgXqWgqlMCk3Rn8z0sIZUV_rHAAyGNJNFRGMtVSsxV9qKotYk4Z3Q.83iPMmNYIls6qgIfasJQUKU- 7, 19 -- Received: from [80.122.101.100] by web37410.mail.mud.yahoo.com via HTTP; Fri, 08 Feb 2008 00:15:32 PST 7, 19 -- X-Mailer: YahooMailRC/818.31 YahooMailWebService/0.7.162 7, 19 -- Date: Fri, 8 Feb 2008 00:15:32 -0800 (PST) 7, 19 -- From: Stephane Nizet {nizets2-at-yahoo.com} 7, 19 -- Subject: [Microscopy] The mercury mystery 7, 19 -- To: microscopy-at-microscopy.com 7, 19 -- MIME-Version: 1.0 7, 19 -- Content-Type: text/plain; charset=us-ascii 7, 19 -- Message-ID: {345314.82936.qm-at-web37410.mail.mud.yahoo.com} ==============================End of - Headers==============================
I know it might sound silly, but why don't you have a go at making your own? You already have the tablet. http://www.bongofish.co.uk/wacom/wacom_pt1.html
I wasn't actually aware of the cintiq until the post on here, I've made do with a regular tablet. After seeing the cintiq kit that wacom sell I think it's superb, then I saw the price, and then I thought I'd bet I could make my own. A couple of searches and I found this website; many people have already done this with their own tablets and I'm about to have a bash too!
Hope this helps
-----Original Message----- X-from: PhillipsT-at-missouri.edu [mailto:PhillipsT-at-missouri.edu] Sent: 06 February 2008 16:34 To: a.a.evans-at-Bradford.ac.uk
I am thinking about buying a Wacom Cintiq monitor/tablet so that I can trace objects during on the screen for Photoshop operations (e.g., area measurements using the Fovea Pro toolkit). I would welcome comments from those using this approach about its ease and success rate. The 6 x 10 inch workspace monitor is ~$1000 while the 17 x 10 is ~$2000. Any thoughts on the size of the tablet or the DTF vs. Cintiq models would also be useful. Thanks, Tom
Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
I have to say, having got so used to a mouse, I find a Wacom standard mouse pad A5 sized tablet and a stylus pen so intuitive as it is controlled just like the mouse (even to the same scale of movement), but with far better resolution - although I have to admit the little Wacom also meets my budget requirements rather well and I'm less fussy about an perfect trace (biological variation being rather greater than an exact outline). Plus MetaMorph can undo [backtrace] so easily.
With a light pen on a 15inch CRT you are moving your arm around a lot more (and back in the 1980/90s the screens were tough glass) - but I was impressed compared the basic 1980s ball mouse - although the image editing was hardware based back then not software, and so was lightening fast.
I wouldn't fancy a stylus on a modern soft fronted CRT screen - my touchscreen PDAs and video camera screens are looking pretty manky after a few years use with a stylus - as does my kids Nintendos, but they at least have a plastic sticky screen protector (that looks naff with bubbles everywhere). I find bending down to trace things a lot more fatiguing that looking horizontally at a vertical PC monitor. Plus the A5 Wacom just hot USB2 unplugs and goes in the drawer afterwards as desktop space is a premium these days. Also Wacom tablets survive a hot cup of coffee and sandwich crumbs with ease - and if they didn't they are also cheaper to replace (stylues pens excepted, they are always getting lost). I guess I just don't like tablet PCs really - handwriting is so last millennium - plus I want plenty of raw power i.e. an imaging workstation desktop [laptops need not apply].
I would be interested to get an update on the Cintiq if you get one - I'm sure a lot of others on the list-server would as well.
Thanks for an interesting post.
Keith
-------------------------------------------------------------------------- Dr keith J. Morris Molecular Cytogenetics and Microscopy Core Laboratory 00/069 and 00/070 The Wellcome Trust Centre for Human Genetics Roosevelt Drive Oxford OX3 7BN United Kingdom Telephone: +44 (0)1865 287568 Email: kjmorris-at-well.ox.ac.uk Web-pages: http://www.well.ox.ac.uk/cytogenetics/
-----Original Message----- X-from: PhillipsT-at-missouri.edu [mailto:PhillipsT-at-missouri.edu] Sent: 07 February 2008 19:52 To: kjmorris-at-well.ox.ac.uk
I haven't used any type of tablet before. I must admit that reading about them in some of the posts makes me wonder why I haven't. It is a less expensive option. But some of the other posts are clearly extolling the Cintiq which allows direct tracing on a near horizontal monitor. I have used a mouse to trace 1000's of objects and I am fairly adept but I know that I would have to be 10x faster tracing an object on a photograph or, in this case, the monitor. I was mostly looking to hear that this was as good as it seems on paper and it sounds like it should be. I am definitely going to bite the bullet and buy either the small or medium size monitor. I found the medium size one for 1900 and probably will go with that. I will eventually post a comment about my results once I get the new toy. I appreciate all the helpful comments. Tom
Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
-----Original Message----- X-from: vladislav_speransky-at-nih.gov [mailto:vladislav_speransky-at-nih.gov] Sent: Thursday, February 07, 2008 11:59 AM To: Phillips, Thomas E.
Hello Tom and Keith,
Keith, thanks for a thorough and informative post! And Tom, have you actually been using a tablet already and now are considering an upgrade to Cintiq? Or you haven't use either yet?
Anyway, I have just one comment to add now: that our hand (even my clumsy one) seems to adjust very easily to any scaling when you trace with a Wacom pen on your tablet while looking at the screen. Seriously, I remember I was surprised at first. The hand learns immediately and has no trouble following the outline, even if the magnification on the screen is much higher. That means that bigger tablet may not be better. Think about how exactly you will be using it - setting on your desk in front of the monitor? or on your lap? on your knee sitting with your legs crossed? We found Wacom's second small size to be optimal. Bigger tablet can feel really bulky and harder to balance; besides, there are those buttons on the tablet's sides, and you won't be able to reach them as easy.
Vlad ________________________________________________ Vlad Speransky, Staff Scientist Supramolecular Structure and Function Resource National Institute of Biomedical Imaging and Bioengineering, NIH 13 South Dr, Rm. 3N17 MSC 5766 Bethesda, MD 20892 301 496-3989 vladislav_speransky-at-nih.gov
==============================Original Headers============================== 5, 22 -- From vladislav_speransky-at-nih.gov Thu Feb 7 11:57:56 2008 5, 22 -- Received: from nihrelayxway3.hub.nih.gov (nihrelayxway3.hub.nih.gov [128.231.90.108]) 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m17HvtHn002067 5, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 7 Feb 2008 11:57:55 -0600 5, 22 -- X-IronPortListener: NIH_Relay 5, 22 -- X-SBRS: None 5, 22 -- X-IronPort-AV: E=Sophos;i="4.25,316,1199682000"; 5, 22 -- d="scan'208";a="300074426" 5, 22 -- Received: from unknown (HELO helix.nih.gov) ([128.231.90.83]) 5, 22 -- by nihrelayxway3.hub.nih.gov with ESMTP; 07 Feb 2008 12:57:56 -0500 5, 22 -- Received: from [128.231.217.77] (db477.niaid.nih.gov [128.231.217.77]) 5, 22 -- by helix.nih.gov (8.13.6/8.13.6/2SCANNER) with ESMTP id m17HvtTV21648775 5, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 7 Feb 2008 12:57:55 -0500 (EST) 5, 22 -- Mime-Version: 1.0 (Apple Message framework v753) 5, 22 -- Content-Transfer-Encoding: 7bit 5, 22 -- Message-Id: {4CE9FF05-2EF1-42AE-B116-D26BA3D66A52-at-nih.gov} 5, 22 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 5, 22 -- To: Microscopy-at-microscopy.com 5, 22 -- From: Vlad Speransky {vladislav_speransky-at-nih.gov} 5, 22 -- Subject: [Microscopy] RE: Wacom tablets/touch-screen monitors 5, 22 -- Date: Thu, 7 Feb 2008 12:57:37 -0500 5, 22 -- X-Mailer: Apple Mail (2.753) ==============================End of - Headers==============================
==============================Original Headers============================== 16, 26 -- From PhillipsT-at-missouri.edu Thu Feb 7 13:39:10 2008 16, 26 -- Received: from um-tsmtpout1.um.umsystem.edu (um-tsmtpout1.um.umsystem.edu [209.106.228.23]) 16, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m17JdAOi030895 16, 26 -- for {Microscopy-at-msa.microscopy.com} ; Thu, 7 Feb 2008 13:39:10 -0600 16, 26 -- Received: from UM-XMAIL06.um.umsystem.edu ([209.106.228.32]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.3959); 16, 26 -- Thu, 7 Feb 2008 13:39:09 -0600 16, 26 -- x-mimeole: Produced By Microsoft Exchange V6.5 16, 26 -- Content-class: urn:content-classes:message 16, 26 -- MIME-Version: 1.0 16, 26 -- Content-Type: text/plain; 16, 26 -- charset="us-ascii" 16, 26 -- Subject: RE: [Microscopy] RE: Wacom tablets/touch-screen monitors 16, 26 -- Date: Thu, 7 Feb 2008 13:39:07 -0600 16, 26 -- Message-ID: {0510DC719E56F64BB2AD84EE64CE6BAD038C96E1-at-UM-XMAIL06.um.umsystem.edu} 16, 26 -- In-Reply-To: {200802071759.m17Hx6SO003334-at-ns.microscopy.com} 16, 26 -- X-MS-Has-Attach: 16, 26 -- X-MS-TNEF-Correlator: 16, 26 -- Thread-Topic: [Microscopy] RE: Wacom tablets/touch-screen monitors 16, 26 -- Thread-Index: AchpsyjSCQHx2QGeTue5OKjW/VH0WwADVTDg 16, 26 -- References: {200802071759.m17Hx6SO003334-at-ns.microscopy.com} 16, 26 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu} 16, 26 -- To: {vladislav_speransky-at-nih.gov} 16, 26 -- Cc: {Microscopy-at-msa.microscopy.com} 16, 26 -- X-OriginalArrivalTime: 07 Feb 2008 19:39:09.0951 (UTC) FILETIME=[1C0148F0:01C869C1] 16, 26 -- Content-Transfer-Encoding: 8bit 16, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m17JdAOi030895 ==============================End of - Headers==============================
==============================Original Headers============================== 30, 22 -- From kjmorris-at-well.ox.ac.uk Fri Feb 8 03:41:41 2008 30, 22 -- Received: from morse.well.ox.ac.uk (morse.well.ox.ac.uk [129.67.44.2]) 30, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m189fbU5008790 30, 22 -- for {Microscopy-at-Microscopy.Com} ; Fri, 8 Feb 2008 03:41:38 -0600 30, 22 -- Received: from dhcp079.well.ox.ac.uk ([129.67.44.178] helo=CytoWhizz) 30, 22 -- by morse.well.ox.ac.uk with esmtp (Exim 4.52) 30, 22 -- id 1JNPjk-00039Z-W4 30, 22 -- for Microscopy-at-Microscopy.Com; Fri, 08 Feb 2008 09:41:37 +0000 30, 22 -- From: "Keith Morris" {kjmorris-at-well.ox.ac.uk} 30, 22 -- To: {Microscopy-at-Microscopy.Com} 30, 22 -- References: {200802071952.m17Jq3sp010657-at-ns.microscopy.com} 30, 22 -- Subject: RE: [Microscopy] RE: RE: Wacom tablets/touch-screen monitors 30, 22 -- Date: Fri, 8 Feb 2008 09:41:57 -0000 30, 22 -- Message-ID: {000901c86a36$d8e45500$b22c4381-at-CytoWhizz} 30, 22 -- MIME-Version: 1.0 30, 22 -- Content-Type: text/plain; 30, 22 -- charset="us-ascii" 30, 22 -- Content-Transfer-Encoding: 7bit 30, 22 -- X-Mailer: Microsoft Office Outlook 11 30, 22 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 30, 22 -- Thread-Index: AchpwulfBvyYUz37TFCmi3ahbZZK6AAbx/Yg 30, 22 -- In-Reply-To: {200802071952.m17Jq3sp010657-at-ns.microscopy.com} ==============================End of - Headers==============================
I am planning my 2008 year professional development right now, and I would like to take a short (but intensive) course in SEM imaging. I would like to learn how to bring out better images, as well as some sample preparation techniques for biological samples. I can get away for up to a week for a course, possibly with return visits at a later date for follow-up. If anyone has taken such a course, or offers one, please send your comments to me off-list.
Thanks,
Justin A. Kraft
==============================Original Headers============================== 3, 27 -- From kraftpiano-at-gmail.com Fri Feb 8 07:28:27 2008 3, 27 -- Received: from hs-out-2122.google.com (hs-out-0708.google.com [64.233.178.246]) 3, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m18DSQ4r029395 3, 27 -- for {microscopy-at-microscopy.com} ; Fri, 8 Feb 2008 07:28:27 -0600 3, 27 -- Received: by hs-out-2122.google.com with SMTP id 55so2231296hsc.2 3, 27 -- for {microscopy-at-microscopy.com} ; Fri, 08 Feb 2008 05:28:26 -0800 (PST) 3, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 3, 27 -- d=gmail.com; s=gamma; 3, 27 -- h=domainkey-signature:received:received:message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 3, 27 -- bh=3pv8xHOR3W+r5uFAlFwkuUQ8MvP/js89aPiz8V3PLiU=; 3, 27 -- b=VOrT1pspi1ZLxxQDkzYBQs30PX7lT1NTJkkVRGDwj+HC9IMHBoyMrHSsCPEKf8Vza0hvB1FEG5H2ON8tQv5wyK9D6h+hamU+BxwTPTe7BGMz7iFQhlKQEWiykTcMyfDn+QxxQ2QIC6AlmnplOJff+3Sq8xLIOYHFEsGGIfhudIo= 3, 27 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 3, 27 -- d=gmail.com; s=gamma; 3, 27 -- h=message-id:date:from:to:subject:mime-version:content-type:content-transfer-encoding:content-disposition; 3, 27 -- b=ZW0F8u9UadW4y29Hz08OGgkHM2IQN23l8N8PYOsoU6w5w3DvUvxHQfBko7TP0IskfHcsAKo8SS7ExJunpdO7ozMh+UlljEjYmZiW9S/njz+M7d03TSvywTmgQEBW6cay7mkkUMTLd9akQaIlVlN1WlwUQ+FlKodXoHwF2LkbKSo= 3, 27 -- Received: by 10.141.206.13 with SMTP id i13mr8481525rvq.211.1202477305311; 3, 27 -- Fri, 08 Feb 2008 05:28:25 -0800 (PST) 3, 27 -- Received: by 10.141.13.19 with HTTP; Fri, 8 Feb 2008 05:28:25 -0800 (PST) 3, 27 -- Message-ID: {25e2b0d20802080528g65072e1bw7f4a894d2155fb03-at-mail.gmail.com} 3, 27 -- Date: Fri, 8 Feb 2008 08:28:25 -0500 3, 27 -- From: "Justin Kraft" {kraftpiano-at-gmail.com} 3, 27 -- To: microscopy-at-microscopy.com 3, 27 -- Subject: SEM Short Course recommendations. 3, 27 -- MIME-Version: 1.0 3, 27 -- Content-Type: text/plain; charset=ISO-8859-1 3, 27 -- Content-Transfer-Encoding: 7bit 3, 27 -- Content-Disposition: inline ==============================End of - Headers==============================
----- Original Message ---- X-from: "A.A.Evans-at-Bradford.ac.uk" {A.A.Evans-at-Bradford.ac.uk} To: nizets2-at-yahoo.com Sent: Friday, February 8, 2008 9:39:31 AM
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____________________________________________________________________________________ Looking for last minute shopping deals? Find them fast with Yahoo! Search. http://tools.search.yahoo.com/newsearch/category.php?category=shopping
____________________________________________________________________________________ Looking for last minute shopping deals? Find them fast with Yahoo! Search. http://tools.search.yahoo.com/newsearch/category.php?category=shopping
==============================Original Headers============================== 35, 19 -- From nizets2-at-yahoo.com Fri Feb 8 07:43:16 2008 35, 19 -- Received: from web37405.mail.mud.yahoo.com (web37405.mail.mud.yahoo.com [209.191.91.137]) 35, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m18DhGLl010046 35, 19 -- for {microscopy-at-microscopy.com} ; Fri, 8 Feb 2008 07:43:16 -0600 35, 19 -- Received: (qmail 45074 invoked by uid 60001); 8 Feb 2008 13:43:16 -0000 35, 19 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 35, 19 -- s=s1024; d=yahoo.com; 35, 19 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Message-ID; 35, 19 -- b=yvzB1fhWrLCTEjVjxuDI1OZdOb+48GuKHPyVNzdq/cL6GR9ZpU3Xo1s7aeZ6VjU5CbvAuKJA0MQgmiGCijSAOhIQliOZ6/gayJjliC+oEunXPr0u488uj3WIkiWmFk2fQ6onfABRaWf+ko7Bcfp4G3WC2D3gm2n8M0OTj4cXBKE=; 35, 19 -- X-YMail-OSG: NfS_BpsVM1lBNjhWS3jzANGMW6FCX38RismqrT5rpvUTmZmZZAGZiNN1lLWwgeAP2fM1B_JshqjhnmZ4f90cJqKhbl2G56MPcE2pSfhdEiLV_g9PEjjcRpBG4fk1SNovBtTJgmw7S4MpTpqeZ04Nv.9d.VXuN2TbIypQTWlbFQ9c 35, 19 -- Received: from [80.121.2.186] by web37405.mail.mud.yahoo.com via HTTP; Fri, 08 Feb 2008 05:43:15 PST 35, 19 -- X-Mailer: YahooMailRC/818.31 YahooMailWebService/0.7.162 35, 19 -- Date: Fri, 8 Feb 2008 05:43:15 -0800 (PST) 35, 19 -- From: Stephane Nizet {nizets2-at-yahoo.com} 35, 19 -- Subject: Re: [Microscopy] Wacom tablets/touch-screen monitors 35, 19 -- To: microscopy-at-microscopy.com 35, 19 -- MIME-Version: 1.0 35, 19 -- Content-Type: text/plain; charset=us-ascii 35, 19 -- Message-ID: {118138.43483.qm-at-web37405.mail.mud.yahoo.com} ==============================End of - Headers==============================
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Lockheed Martin- KAPL, Inc. has an open position for a surface scientist (experienced in Auger / ESCA techniques). A brief description of the job is given below. NOTE: US Citizenship REQUIRED.
The complete job announcement (Req ID 66233BR) can be found at the Lockheed Martin career web site (LockheedMartin.com).
Jim McGee ************************************ James J. McGee Materials Engineer, Test Operations Lockheed Martin, KAPL, Inc. Mail Bin 149 PO Box 1072 Schenectady, NY 12301-1072
Required skills: MS degree in the physical sciences (e.g. materials science, chemistry, geology, solid state physics) or engineering, plus hands-on training and/or experience operating scanning Auger electron microscopy (SAM), Electron Spectroscopy for Chemical Analysis (ESCA), and Secondary Ion Mass Spectrometry (SIMS). Desired skills PhD degree in physical sciences with 5 years or more experience in surface and microanalysis (SAM, ESCA, and SIMS) and demonstrated problem solving experience in areas of metallurgy, alloy testing/development, solid inorganic materials, and/or ceramics. Previous experience and nuclear or radioactive materials, corrosion, metallurgy, and ceramics is a also desirable.
Specific Job Description: Characterize the surfaces of materials to support environmental testing and failure analysis of in-service components. Utilize field-emission Auger electron microscopy and imaging, ESCA spectroscopy and imaging, and SIMS. Execute data and image processing (e.g. Target-Factor Analysis (TFA, PCA), curve fitting, quantitative analysis, data assimilation, and reporting of results. Participate in multidisciplinary, collaborative investigations and team-oriented problem solving with other materials characterization specialists and materials scientists/engineers using associated analytical techniques (FEG-SEM, EBSD, EPMA, XRD, TEM, FTIR, Raman, FIB, TOF-SIMS).
The major duties will be the use of advanced surface analytical techniques to determine the microstructure and microchemistry of metals, alloys, and ceramic materials. Ensure instrumentation is properly maintained and in proper operating condition. Analyze and interpret data and communicate results to sponsoring groups by oral and written reports. Serve as part of research teams to design experiments that will elucidate structure-composition-property-processing relationships. Stay current in surface and microanalytical techniques and applications and propose/implement new capabilities or characterization tools.
==============================Original Headers============================== 14, 28 -- From mcgeejj-at-kapl.gov Fri Feb 8 08:22:47 2008 14, 28 -- Received: from mars.bias2000.bettis.gov (bettis.gov [65.170.176.212]) 14, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m18EMkMN023867 14, 28 -- for {microscopy-at-microscopy.com} ; Fri, 8 Feb 2008 08:22:46 -0600 14, 28 -- Received: from atlas.bias2000.bettis.gov (unverified) by mars.bias2000.bettis.gov 14, 28 -- (Clearswift SMTPRS 5.0.9) with SMTP id {T8504c37ef7ac1002681698-at-mars.bias2000.bettis.gov} for {microscopy-at-microscopy.com} ; 14, 28 -- Fri, 8 Feb 2008 09:22:51 -0500 14, 28 -- Received: from ibetpex02.bias2000.bettis.gov ([172.16.2.129]) by atlas.bias2000.bettis.gov with Microsoft SMTPSVC(6.0.3790.1830); 14, 28 -- Fri, 8 Feb 2008 09:22:50 -0500 14, 28 -- Content-class: urn:content-classes:message 14, 28 -- MIME-Version: 1.0 14, 28 -- Content-Type: text/plain; 14, 28 -- charset="us-ascii" 14, 28 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 14, 28 -- Subject: Open Position - Surface scientist for materials science applications 14, 28 -- Date: Fri, 8 Feb 2008 09:22:50 -0500 14, 28 -- Message-ID: {8807BA5B50FE0E40957843C1A36ACB83B36342-at-ibetpex02.bias2000.bettis.gov} 14, 28 -- In-reply-to: {8807BA5B50FE0E40957843C1A36ACB833D087D-at-ibetpex02.bias2000.bettis.gov} 14, 28 -- X-MS-Has-Attach: 14, 28 -- X-MS-TNEF-Correlator: 14, 28 -- Thread-Topic: Open Position - Surface scientist for materials science applications 14, 28 -- Thread-Index: Acg7M8tz2LXeTCaQQxK7z5idvCoucQvKUYTA 14, 28 -- References: {8807BA5B50FE0E40957843C1A36ACB833D087D-at-ibetpex02.bias2000.bettis.gov} 14, 28 -- From: "McGee, James" {mcgeejj-at-kapl.gov} 14, 28 -- To: {microscopy-at-microscopy.com} 14, 28 -- X-OriginalArrivalTime: 08 Feb 2008 14:22:50.0730 (UTC) FILETIME=[15EBBCA0:01C86A5E] 14, 28 -- Content-Transfer-Encoding: 8bit 14, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m18EMkMN023867 ==============================End of - Headers==============================
The math would fit for the escape of Al x-rays during the detection of Ca (3.69 - 1.49). I wouldn't ordinarily expect that you would detect that sort of an escape peak, but at 70 dead time, who knows...? When your colleague cranked the dead time, did you also detect a new peak at 1.95, corresponding to Ca minus Si? If your mystery peak is an escape peak, I would think you would see this one as well.
Yours, Matt
Matthew Stephenson Impact Analytical/MMI 1910 W. Saint Andrews Rd. Midland, MI 48640 (989)832-5555 X506 stephenson-at-impactanalytical.com Visit Impact Analytical in Booth # 3305 at PittCon March 3rd - 7th
-----Original Message----- X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com] Sent: Friday, February 08, 2008 3:23 AM To: stephenson-at-impactanalytical.com
Hi all!
No I don't present my last book, I just need your help to solve a mysterious peak in EDX ;-) Here is the story: A colleague was analysing a piece of zeolite in SEM with EDX (Tescan SEM, Oxford Instr. EDX). The specimen was sputter-coated with carbon and the HT was 20 keV (W filament). I told her to try stick with a deadtime of about 20% and adjusted the parameters accordingly. At that moment we had the nice Al peak (1,49 keV), Si peak (1,74 keV) and a smaller but very clear Ca peak (3,69 keV). Happyness at its best. Then I left her to have a cup of coffee...eer I mean to study a very difficult case and when I was back she said she found some mercury in the sample! (which is absolutely unexpected even as contaminant). She had increased the spot size and had a deadtime of more than 70%!! And indeed she had a very tiny Hg peak at 2,19 keV! All others parameters being the same, when I decreased the spot size the Hg peak disappeared. This is obviously an artifact, but after reading the book from Goldstein, Newbury at al. I cannot find to which artifact belongs that Hg peak. Any thought about it?
Best regards,
Stephane
PS: I will summarize the answers I got from my question about the IGP pump replacement soon
____________________________________________________________________________ ________ Never miss a thing. Make Yahoo your home page. http://www.yahoo.com/r/hs
==============================Original Headers============================== 7, 19 -- From nizets2-at-yahoo.com Fri Feb 8 02:15:33 2008 7, 19 -- Received: from web37410.mail.mud.yahoo.com (web37410.mail.mud.yahoo.com [209.191.91.142]) 7, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m188FWUP013082 7, 19 -- for {microscopy-at-microscopy.com} ; Fri, 8 Feb 2008 02:15:32 -0600 7, 19 -- Received: (qmail 83548 invoked by uid 60001); 8 Feb 2008 08:15:32 -0000 7, 19 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 7, 19 -- s=s1024; d=yahoo.com; 7, 19 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Ty pe:Message-ID; 7, 19 -- b=ZuHUvPzVvVcxTFeG/DZUKSUZzfXJBzbC2EligDPZuZSzGDp4Mb0sUIRO0Lh4loLr2QhuZxiokd nkAiLfSdv1Fel1wI3WHvn7WcOSPKBqJwjlPJfP3lelZHP6Engxx8ID1+6J9jh1paTTX1pQyPVYYU g/6OoA1RZjC+mYUb3WABM=; 7, 19 -- X-YMail-OSG: rwxGQEEVM1kny9k7VgIi2iKvuAvYQTepPc_QTIfgXqWgqlMCk3Rn8z0sIZUV_rHAAyGNJNFRGMtV SsxV9qKotYk4Z3Q.83iPMmNYIls6qgIfasJQUKU- 7, 19 -- Received: from [80.122.101.100] by web37410.mail.mud.yahoo.com via HTTP; Fri, 08 Feb 2008 00:15:32 PST 7, 19 -- X-Mailer: YahooMailRC/818.31 YahooMailWebService/0.7.162 7, 19 -- Date: Fri, 8 Feb 2008 00:15:32 -0800 (PST) 7, 19 -- From: Stephane Nizet {nizets2-at-yahoo.com} 7, 19 -- Subject: [Microscopy] The mercury mystery 7, 19 -- To: microscopy-at-microscopy.com 7, 19 -- MIME-Version: 1.0 7, 19 -- Content-Type: text/plain; charset=us-ascii 7, 19 -- Message-ID: {345314.82936.qm-at-web37410.mail.mud.yahoo.com} ==============================End of - Headers==============================
==============================Original Headers============================== 16, 26 -- From stephenson-at-impactanalytical.com Fri Feb 8 08:56:16 2008 16, 26 -- Received: from sa1.mitcon.org (sa1.mitcon.org [192.251.56.4]) 16, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m18EuGON005155 16, 26 -- for {microscopy-at-microscopy.com} ; Fri, 8 Feb 2008 08:56:16 -0600 16, 26 -- Received: from localhost (localhost [127.0.0.1]) 16, 26 -- by sa1.mitcon.org (Postfix) with ESMTP id 71D8B58967 16, 26 -- for {microscopy-at-microscopy.com} ; Fri, 8 Feb 2008 09:56:16 -0500 (EST) 16, 26 -- X-Virus-Scanned: by amavisd-new-2.5.2 (20070627) (Debian) at mitcon.org 16, 26 -- Received: from sa1.mitcon.org ([127.0.0.1]) 16, 26 -- by localhost (sa1.mitcon.org [127.0.0.1]) (amavisd-new, port 10024) 16, 26 -- with ESMTP id yhkowhGbtSV5 for {microscopy-at-microscopy.com} ; 16, 26 -- Fri, 8 Feb 2008 09:56:14 -0500 (EST) 16, 26 -- X-Greylist: whitelisted by SQLgrey-1.6.8 16, 26 -- Received: from mitconexch.mitcon.org (mitconexch.mitcon.org [10.10.10.2]) 16, 26 -- by sa1.mitcon.org (Postfix) with ESMTP id 862E858964 16, 26 -- for {microscopy-at-microscopy.com} ; Fri, 8 Feb 2008 09:56:14 -0500 (EST) 16, 26 -- Received: by mitconexch.mitcon.org with Internet Mail Service (5.5.2657.72) 16, 26 -- id {1J7CQV8L} ; Fri, 8 Feb 2008 09:56:14 -0500 16, 26 -- Message-ID: {089C7709BE9235448E3622C6F38D828F06C6959C-at-mitconexch.mitcon.org} 16, 26 -- From: stephenson-at-impactanalytical.com 16, 26 -- To: microscopy-at-microscopy.com 16, 26 -- Subject: RE: [Microscopy] The mercury mystery 16, 26 -- Date: Fri, 8 Feb 2008 09:56:10 -0500 16, 26 -- MIME-Version: 1.0 16, 26 -- X-Mailer: Internet Mail Service (5.5.2657.72) 16, 26 -- Content-Type: text/plain ==============================End of - Headers==============================
Good Morning: I have a potential project to compare the mechanical properties of collagen in the kneecap with its structure and arrangement. First I'm not sure this is a TEM project as another type of microscopy might be better. Secondly I assume I would have to embed and section the patella and could really use some help on the "how to". If anyone has experience working with this material I would be pleased to get some advice. Thanks bob harris
Guelph Regional Integrated Imaging Facility (GRIIF) Transmission Electron Microscope Facility Dept. of Molecular and Cell Biology New Science Complex, 488 Gordon St. University of Guelph Guelph Ontario, Canada, N1G 2W1 Phone: 519-824-4120 X 56409 Fax: 519-837-1802
==============================Original Headers============================== 5, 26 -- From bharris-at-uoguelph.ca Fri Feb 8 09:28:31 2008 5, 26 -- Received: from dargo.cs.uoguelph.ca (dargo.cs.uoguelph.ca [131.104.94.197]) 5, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m18FSUqi018813 5, 26 -- for {microscopy-at-microscopy.com} ; Fri, 8 Feb 2008 09:28:30 -0600 5, 26 -- Received: from aragorn.cs.uoguelph.ca (css.webmail.uoguelph.ca [131.104.93.20]) 5, 26 -- by dargo.cs.uoguelph.ca (8.13.1/8.13.1) with ESMTP id m18FSNCg003666 5, 26 -- for {microscopy-at-microscopy.com} ; Fri, 8 Feb 2008 10:28:23 -0500 5, 26 -- Received: from webmail.uoguelph.ca (localhost.localdomain [127.0.0.1]) 5, 26 -- by aragorn.cs.uoguelph.ca (8.13.1/8.13.1) with ESMTP id m18FSNsu011443 5, 26 -- for {microscopy-at-microscopy.com} ; Fri, 8 Feb 2008 10:28:23 -0500 5, 26 -- Received: from 131.104.190.70 ([131.104.190.70]) by webmail.uoguelph.ca 5, 26 -- (Horde MIME library) with HTTP; Fri, 08 Feb 2008 10:28:23 -0500 5, 26 -- Message-ID: {20080208102823.yk0o50vlcs4sko48-at-webmail.uoguelph.ca} 5, 26 -- Date: Fri, 08 Feb 2008 10:28:23 -0500 5, 26 -- From: Robert J Harris {bharris-at-uoguelph.ca} 5, 26 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 5, 26 -- Subject: TEM: Collagen in the Patella 5, 26 -- MIME-Version: 1.0 5, 26 -- Content-Type: text/plain; 5, 26 -- charset=ISO-8859-1; 5, 26 -- DelSp="Yes"; 5, 26 -- format="flowed" 5, 26 -- Content-Disposition: inline 5, 26 -- Content-Transfer-Encoding: 7bit 5, 26 -- User-Agent: Internet Messaging Program (IMP) H3 (4.1.1) 5, 26 -- X-Scanned-By: MIMEDefang 2.63 on 131.104.94.197 ==============================End of - Headers==============================
Dear listers, Below please find a description of a postdoctoral opportunity at Oak Ridge National Laboratory. Feel free to post or forward to any qualified candidates.
*Combined Scanning Transmission and Scanning Probe Microscopy of Ferroelectrics*
*Materials Science and Technology Division *
*Oak Ridge National Laboratory *
*Oak Ridge, Tennessee *
*Project Description: *
The Materials Science and Technology Division at Oak Ridge National Laboratory (ORNL) is seeking a candidate to fill a postdoctoral position in the field of combined scanning transmission electron microscopy and scanning probe microscopy of ferroelectric thin films. The position is available immediately. This program takes advantage of ORNL’s suite of advanced electron microscopes, including 4 aberration-corrected instruments, as well as recently acquired (S)TEM/STM and (S)TEM/AFM capabilities.
The successful applicant must demonstrate experience in electron microscope operation, preferably FEI microscopes, as well as skills in analysis and interpretation of microscopic and spectroscopic data. This position provides an opportunity to join an experienced team working in a highly collaborative environment on the projects ranging from heterogeneous catalysis to oxide materials to semiconductors. Close interactions with the scanning probe microscopy program at ORNL’s Center for Nanophase Materials Sciences (CNMS) are anticipated.
*Qualifications: PhD degree required *
This position requires a Ph.D. in Materials Science, Physics, or related field, with an emphasis on advanced TEM or STEM. Knowledge of oxide crystal chemistry is a plus. Excellent oral and written communication skills are required, and presentations and publication of scientific results in peer-reviewed journals are expected. The applicant must have the ability to work in a team and interact effectively with a broad range of colleagues. Applicants cannot have received the most recent degree more than five years prior to the date of application and must complete all degree requirements before starting their appointment.
*How to Apply: *
Qualified applicants may apply online at https://www2.orau.gov/ORNL_POST/ .
All applicants will need to register before they can begin the online application. For complete instructions, on how to apply, please see the instructions at
http://www.orau.gov/orise/edu/ornl/ornl-pdpm/application.htm . When applying for this position, please reference the position title and number ORNL08-23-MSTD). Questions regarding the position can be directed to Drs. Albina Y. Borisevich, albinab-at-ornl.gov {mailto:albinab-at-ornl.gov} , Stephen J. Pennycook, pennycooksj-at-ornl.gov {mailto:pennycooksj-at-ornl.gov} , and Sergei V. Kalinin, sergei2-at-ornl.gov {mailto:sergei2-at-ornl.gov} . Applications will be accepted until the position is filled. This appointment is offered through the ORNL Postgraduate Research Participation Program and is administered by the Oak Ridge Institute for Science and Education (ORISE). The program is open to all qualified U.S. and non-U.S. citizens without regard to race, color, age, religion, sex, national origin, physical or mental disability, or status as a Vietnam-era veteran or disabled veteran.
-- -- Albina Y. Borisevich R&D Associate Electron Microscopy Group Oak Ridge National Laboratory Materials Science and Technology Division PO Box 2008 Oak Ridge TN 37831-6031
http://stem.ornl.gov/
For express mail add: 1 Bethel Valley Road phone: (865) 576-4060 fax: (865) 574-4143
==============================Original Headers============================== 17, 25 -- From albinab-at-ornl.gov Fri Feb 8 14:48:18 2008 17, 25 -- Received: from emroute1.ornl.gov (emroute1.ornl.gov [160.91.4.119]) 17, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m18KmIrJ012567 17, 25 -- for {microscopy-at-microscopy.com} ; Fri, 8 Feb 2008 14:48:18 -0600 17, 25 -- Received: from emroute1.ornl.gov ([127.0.0.1]) 17, 25 -- by emroute1.ornl.gov (PMDF V6.3-x14 #31501) 17, 25 -- with ESMTP id {0JVX0052CTSHE9-at-emroute1.ornl.gov} for 17, 25 -- microscopy-at-microscopy.com; Fri, 08 Feb 2008 15:48:18 -0500 (EST) 17, 25 -- Received: from CONVERSION-DAEMON.emroute1.ornl.gov by emroute1.ornl.gov 17, 25 -- (PMDF V6.3-x14 #31501) id {0JVX00601TSH6H-at-emroute1.ornl.gov} for 17, 25 -- microscopy-at-microscopy.com; Fri, 08 Feb 2008 15:48:17 -0500 (EST) 17, 25 -- Received: from [160.91.48.156] (simulations1.ornl.gov [160.91.48.156]) 17, 25 -- by emroute1.ornl.gov (PMDF V6.3-x14 #31501) 17, 25 -- with ESMTPSA id {0JVX0017RTSHY6-at-emroute1.ornl.gov} for 17, 25 -- microscopy-at-microscopy.com; Fri, 08 Feb 2008 15:48:17 -0500 (EST) 17, 25 -- Date: Fri, 08 Feb 2008 15:48:17 -0500 17, 25 -- From: Albina Borisevich {albinab-at-ornl.gov} 17, 25 -- Subject: Postdoctoral position in Combined Scanning Transmission and Scanning 17, 25 -- Probe Microscopy of Ferroelectrics 17, 25 -- To: microscopy-at-microscopy.com 17, 25 -- Message-id: {47ACC011.9010404-at-ornl.gov} 17, 25 -- MIME-version: 1.0 17, 25 -- Content-type: text/plain; charset=UTF-8; format=flowed 17, 25 -- Content-transfer-encoding: 8BIT 17, 25 -- User-Agent: Thunderbird 2.0.0.9 (Windows/20071031) ==============================End of - Headers==============================
I have gone on line today to reserve my room for M&M 2008 and I chose the Double Tree Hotel. The rate for two was $125 instead of $119 as published on the Microscopy web site (I challenged this). There were no rooms available for Wed. and Thurs. at the discounted meeting rate. I inquired about non-discount rooms, not wishing to pack up and move and they were available at $165, if I remember correctly. Luckily the thrifty little voice in my head spoke up and I was able to get a AAA discount and the cost was $151 and $143.
The message from MSA was correct about the rooms going quickly!
Pat Connelly
==============================Original Headers============================== 5, 21 -- From connellyps-at-nhlbi.nih.gov Fri Feb 8 16:50:41 2008 5, 21 -- Received: from NIHCESSMTP2.hub.nih.gov (nihcessmtp2.hub.nih.gov [128.231.90.116]) 5, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m18Mof2X029643 5, 21 -- for {microscopy-at-microscopy.com} ; Fri, 8 Feb 2008 16:50:41 -0600 5, 21 -- Received: from NIHCESMLBX6.nih.gov ([156.40.71.206]) by NIHCESSMTP2.hub.nih.gov with Microsoft SMTPSVC(6.0.3790.1830); 5, 21 -- Fri, 8 Feb 2008 17:50:36 -0500 5, 21 -- Received: from 156.40.71.188 ([156.40.71.188]) by NIHCESMLBX6.nih.gov ([156.40.71.206]) via Exchange Front-End Server mail.nih.gov ([156.40.71.167]) with Microsoft Exchange Server HTTP-DAV ; 5, 21 -- Fri, 8 Feb 2008 22:50:36 +0000 5, 21 -- User-Agent: Microsoft-Entourage/11.3.6.070618 5, 21 -- Date: Fri, 08 Feb 2008 17:47:51 -0500 5, 21 -- Subject: Hotel for M&M 5, 21 -- From: Patricia Connelly {connellyps-at-nhlbi.nih.gov} 5, 21 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 5, 21 -- Message-ID: {C3D24647.1531%connellyps-at-nhlbi.nih.gov} 5, 21 -- Thread-Topic: Hotel for M&M 5, 21 -- Thread-Index: AchqpKJK4PmbVtaXEdyvlAANk2Yv1A== 5, 21 -- Mime-version: 1.0 5, 21 -- Content-type: text/plain; 5, 21 -- charset="US-ASCII" 5, 21 -- Content-transfer-encoding: 7bit 5, 21 -- X-OriginalArrivalTime: 08 Feb 2008 22:50:36.0270 (UTC) FILETIME=[04CC38E0:01C86AA5] ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both friess-at-limedion.de as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: friess-at-limedion.de Name: Frank Frieþ
Organization: LMI
Title-Subject: [Filtered] 6300F/6400F/6600F
Question: Hi,
is there someone here who is running a JEOL 6xxxF Series with the serial (RS232) interface activated ? If it can be read (and maybe even be controled) some parameters like magnification, voltage etc. by a connected computer system (e.g. EDS) this would be an indicater that the serial is working. (as I found out the interface was often not activated when the devices were sold). I would be happy to find someone with a working RS232.
There are a few basic FA books on polymers out there. It seems that no one book covers the topic sufficiently to be "the" reference. The book you now have and the Engel book previously recommended are good. My other favorites: Failure of Plastics and Rubber Products by David Wright Plastics Failure Analysis and Prevention, John Moalli (Editor) Plastics Failure Guide by Myer Ezrin
-- Larry D. Hanke, P.E. Materials Evaluation and Engineering, Inc. Practical Solutions Through Technology and Innovation http://www.mee-inc.com (763) 449-8870
==============================Original Headers============================== 3, 24 -- From hanke-at-mee-inc.com Fri Feb 8 18:05:11 2008 3, 24 -- Received: from mail.namisolutions.com (mail.namisolutions.com [12.40.181.38]) 3, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1905BLu024651 3, 24 -- for {microscopy-at-microscopy.com} ; Fri, 8 Feb 2008 18:05:11 -0600 3, 24 -- Received: (qmail 27771 invoked by uid 508); 8 Feb 2008 18:05:11 -0600 3, 24 -- Received: from 216.14.180.82 by mail.namisolutions.com (envelope-from {hanke-at-mee-inc.com} , uid 507) with qmail-scanner-1.24-st-qms 3, 24 -- (clamdscan: 0.87/5723. spamassassin: 3.0.2. perlscan: 1.24-st-qms. 3, 24 -- Clear:RC:1(216.14.180.82):. 3, 24 -- Processed in 0.050229 secs); 09 Feb 2008 00:05:11 -0000 3, 24 -- X-Antivirus-NAMISOLUTIONS-Mail-From: hanke-at-mee-inc.com via mail.namisolutions.com 3, 24 -- X-Antivirus-NAMISOLUTIONS: 1.24-st-qms (Clear:RC:1(216.14.180.82):. Processed in 0.050229 secs Process 27764) 3, 24 -- Received: from unknown (HELO ?192.168.1.4?) (216.14.180.82) 3, 24 -- by mail.namisolutions.com with SMTP; 8 Feb 2008 18:05:11 -0600 3, 24 -- Message-ID: {47ACEE32.9080100-at-mee-inc.com} 3, 24 -- Date: Fri, 08 Feb 2008 18:05:06 -0600 3, 24 -- From: Larry Hanke {hanke-at-mee-inc.com} 3, 24 -- User-Agent: Thunderbird 2.0.0.9 (Windows/20071031) 3, 24 -- MIME-Version: 1.0 3, 24 -- To: yuhong.wu-at-solvay.com, microscopy-at-microscopy.com 3, 24 -- Subject: Re: [Microscopy] viaWWW: Polymer Failure Analysis book 3, 24 -- References: {200802060523.m165NqpL004269-at-ns.microscopy.com} 3, 24 -- In-Reply-To: {200802060523.m165NqpL004269-at-ns.microscopy.com} 3, 24 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 3, 24 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Dear All, has someone out there a procedure for opening up the column of a 1997 EM 208S at the specimen stage to get a jammed prep-holder out again? Anything special to take care of?
Best regards, Stefan
Aktuelles: "Die Ästhetik des Unsichtbaren" - Pflanzenoberflächen unter dem Elektronenmikroskop Siehe www.elektronenmikroskopie.info/ausstelllungen/wuerzburg Mein Jahreskalender 2008 zum herunterladen und selbst ausdrucken im Format DIN A3: www.quantifoil.com/calendar_2008.pdf (38 MB) Falls Sie Bilder daraus kommerziell verwenden möchten fragen Sie mich bitte vorher...
Here is a summary of the different answers and finally what I think is the right one. I want to thank everybody for their help, I received lots of answers, almost all different but all very interesting.
1) Hg may be an escape peak of Ca. However in my book, and as someone stated, this does not really fit well. Ca (3,69) -1,74 (following my book) is not very near to 2,19 (Hg peak)
2) It is not a contamination, since this Hg peaks appears only at (too) high a deadtime. It clearly indicates an artifact.
3) One comment stated "It's the Ca peak (3.69) exciting the Al peak (1.49) leaving a 2.20 kV X-ray to exit the sample". I don't understand it!! (sorry)
3) The most reasonable answer is a pileup peak of O+Si. The 2 peaks are the 2 highest in the spectrum and their addition perfectly fits to the Hg peak. The person who proposed that solution seems to be experienced with this type of material and problem and this pileup does not seem so uncommon for high deadtimes.
I hope this can help others.
Best regards, Stephane
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==============================Original Headers============================== 10, 19 -- From nizets2-at-yahoo.com Tue Feb 12 05:01:35 2008 10, 19 -- Received: from web37409.mail.mud.yahoo.com (web37409.mail.mud.yahoo.com [209.191.91.141]) 10, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id m1CB1Yvo000426 10, 19 -- for {microscopy-at-microscopy.com} ; Tue, 12 Feb 2008 05:01:34 -0600 10, 19 -- Received: (qmail 79686 invoked by uid 60001); 12 Feb 2008 11:01:34 -0000 10, 19 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 10, 19 -- s=s1024; d=yahoo.com; 10, 19 -- h=X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Message-ID; 10, 19 -- b=sdb/1OOan8CsPMUHncrtuhqj8zYyHcAPSmDXyPjCm5lwwbhnNx79T6WLyjUmI4F01WlChpm93S2hkggQhuG9talchtgqOOvYPOxRT4N4gRLoJKr9/l8NyaKfyhBG/LlwjtJ1Rj78XiNuFzzLCc2uZdP+yOfNowahfwVsp7t/HR4=; 10, 19 -- X-YMail-OSG: 4Z_mM5gVM1myonBevvr8LbyC8CbETsr5OQopq60o42SdJcLD6.Mqkjnc0M9pMxFE848O5mOgCs5Q8mH097Rm8zB50PCE19nCOPx99SgYEPr6b.AzPzY- 10, 19 -- Received: from [80.122.101.100] by web37409.mail.mud.yahoo.com via HTTP; Tue, 12 Feb 2008 03:01:34 PST 10, 19 -- X-Mailer: YahooMailRC/818.31 YahooMailWebService/0.7.162 10, 19 -- Date: Tue, 12 Feb 2008 03:01:34 -0800 (PST) 10, 19 -- From: Stephane Nizet {nizets2-at-yahoo.com} 10, 19 -- Subject: the mercury mystery solved!! 10, 19 -- To: microscopy-at-microscopy.com 10, 19 -- MIME-Version: 1.0 10, 19 -- Content-Type: text/plain; charset=us-ascii 10, 19 -- Message-ID: {494167.73968.qm-at-web37409.mail.mud.yahoo.com} ==============================End of - Headers==============================
I concur with number 3. I strongly suspected O+Si was the explanation. Since I hadn't seen it suggest as an answer, I threw some SiO2 into our older SEM yesterday afternoon and collected spectra at 20%, 30%, and 60% deadtime. I pasted the spectra and analytical results into a file which you may find here (ftp://www.marl.iastate.edu/General/SiO2_with_Hg.pdf ). You can see the "Hg" peak increase with increasing deadtime. You can also see the O+O and Si+Si sum peaks, but the "Hg" O+Si sum peak seems to be the highest in this case.
The analytical results quantify the effect. The Hg mass fraction is reported as 1.3, 1.7 and 6.8%. I did not push the exercise to lower deadtimes. I also did not try tweaking our old Kevex pulse processor to improve its pile-up rejection (for the record, the Kevex feeds an IXRF Systems box). I should repeat this experiment on our newer EDS system and see if it performs any better.
The moral of the story is that sum peaks are one of the main reasons that deadtime needs to be limited. Also, just because you can't see a peak (e.g., O or C) doesn't mean in can't pile up and appear in the spectrum.
Warren Straszheim Materials Analysis and Research Lab Iowa State University
-----Original Message----- X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com] Sent: Tuesday, February 12, 2008 5:03 AM To: wesaia-at-iastate.edu
Dear list,
Here is a summary of the different answers and finally what I think is the right one. I want to thank everybody for their help, I received lots of answers, almost all different but all very interesting.
1) Hg may be an escape peak of Ca. However in my book, and as someone stated, this does not really fit well. Ca (3,69) -1,74 (following my book) is not very near to 2,19 (Hg peak)
2) It is not a contamination, since this Hg peaks appears only at (too) high a deadtime. It clearly indicates an artifact.
3) One comment stated "It's the Ca peak (3.69) exciting the Al peak (1.49) leaving a 2.20 kV X-ray to exit the sample". I don't understand it!! (sorry)
3) The most reasonable answer is a pileup peak of O+Si. The 2 peaks are the 2 highest in the spectrum and their addition perfectly fits to the Hg peak. The person who proposed that solution seems to be experienced with this type of material and problem and this pileup does not seem so uncommon for high deadtimes.
I hope this can help others.
Best regards, Stephane
==============================Original Headers============================== 19, 37 -- From wesaia-at-iastate.edu Tue Feb 12 09:15:23 2008 19, 37 -- Received: from mailhub-3.iastate.edu (mailhub-3.iastate.edu [129.186.140.13]) 19, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1CFFNn1023916 19, 37 -- for {Microscopy-at-msa.microscopy.com} ; Tue, 12 Feb 2008 09:15:23 -0600 19, 37 -- Received: from devirus-10.iastate.edu (devirus-10.iastate.edu [129.186.1.47]) 19, 37 -- by mailhub-3.iastate.edu (8.12.11.20060614/8.12.10) with SMTP id m1CFFNv5018276; 19, 37 -- Tue, 12 Feb 2008 09:15:23 -0600 19, 37 -- Received: from (despam-10.iastate.edu [129.186.140.80]) by devirus-10.iastate.edu with smtp 19, 37 -- id 147f_50b9343c_d97d_11dc_9726_00137253420a; 19, 37 -- Tue, 12 Feb 2008 09:15:16 -0600 19, 37 -- Received: from owa.eng.iastate.edu (owa.eng.iastate.edu [129.186.23.85]) 19, 37 -- by despam-10.iastate.edu (8.12.11.20060614/8.12.10) with ESMTP id m1CFFGnv027152; 19, 37 -- Tue, 12 Feb 2008 09:15:21 -0600 19, 37 -- Received: from maire.eng.iastate.edu ([10.10.196.69]) by owa.eng.iastate.edu with Microsoft SMTPSVC(6.0.3790.3959); 19, 37 -- Tue, 12 Feb 2008 09:15:09 -0600 19, 37 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 19, 37 -- Content-class: urn:content-classes:message 19, 37 -- MIME-Version: 1.0 19, 37 -- Content-Type: text/plain; 19, 37 -- charset="us-ascii" 19, 37 -- Subject: RE: [Microscopy] the mercury mystery solved!! 19, 37 -- Date: Tue, 12 Feb 2008 09:15:20 -0600 19, 37 -- Message-ID: {16A330AC32056A40B32842EC4BB8D727028845D3-at-maire.eng.iastate.edu} 19, 37 -- In-Reply-To: {200802121102.m1CB2rwt001699-at-ns.microscopy.com} 19, 37 -- X-MS-Has-Attach: 19, 37 -- X-MS-TNEF-Correlator: 19, 37 -- Thread-Topic: [Microscopy] the mercury mystery solved!! 19, 37 -- Thread-Index: AchtZteWLKGGWczDQ0C7FkvLJ5KnvQAHM+qA 19, 37 -- References: {200802121102.m1CB2rwt001699-at-ns.microscopy.com} 19, 37 -- From: "Straszheim, Warren E [M S E]" {wesaia-at-iastate.edu} 19, 37 -- To: "MSA" {Microscopy-at-msa.microscopy.com} 19, 37 -- Cc: {nizets2-at-yahoo.com} 19, 37 -- X-OriginalArrivalTime: 12 Feb 2008 15:15:09.0402 (UTC) FILETIME=[0E5E13A0:01C86D8A] 19, 37 -- X-PMX-Version: 5.3.2.304607, Antispam-Engine: 2.5.1.298604, Antispam-Data: 2008.2.12.70212 19, 37 -- X-ISUMailhub-test: Gauge=IIIIIII, Probability=7%, Report='ECARD_KNOWN_DOMAINS 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __IMS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __STOCK_SUBJ_9 0' 19, 37 -- Content-Transfer-Encoding: 8bit 19, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id m1CFFNn1023916 ==============================End of - Headers==============================
You can find a fairly detailed description of various vacuum greases in Sect. 10.12 (p.458) of my book 'Vacuum Methods in Electron Microscopy' I would recommend one of the perfluorinated polyphenyl ether greases Brayco 803 or Krytox LVP for most applications in electron microscopy. These greases are excellent lubricants, and are chemically inert (and so should not cause any corrosive reactions with metal parts), and have vapor pressures in the 10^-10 Torr range. However, I have recently learned of a grease called Santovac-5GB that is based on the polyphenyl ether vacuum fluid Santovac-5 which also has excellent properties (vapor pressure of 4 x 10^-10 Torr, stable up to 450 C, excellent as a lubricant, no tendency to spread or bleed, etc.) and which might be equally good. Some people prefer not to use the perfluorinated compounds in their instruments, and so this might be a good alternative choice. You can find a detailed description of both types of grease in the SPI online catalog. The perfluorinated polyphenyl ether diffusion pump fluids Fomblin Y-25/9 and Krytox 1625 are also very good lubricants, particularly for O-rings on rotating shafts. -- Wilbur C. Bigelow, Professor Emeritus Materials Sci. & Engr., Univ. of Michigan Ann Arbor, Michigan 48109-2136 e-mail: bigelow-at-umich.edu; Fx:734-763-4788; Ph:734-975-0858 Address mail to: 2911 Whittier Court Ann Arbor, MI 48104-6731
==============================Original Headers============================== 1, 14 -- From bigelow-at-umich.edu Tue Feb 12 14:20:07 2008 1, 14 -- Received: from hellskitchen.mr.itd.umich.edu (smtp.mail.umich.edu [141.211.14.82]) 1, 14 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1CKK7bA015436 1, 14 -- for {microscopy-at-microscopy.com} ; Tue, 12 Feb 2008 14:20:07 -0600 1, 14 -- Received: FROM [141.212.131.221] (bigelow-g4.engin.umich.edu [141.212.131.221]) 1, 14 -- BY hellskitchen.mr.itd.umich.edu ID 47B1FF75.490E8.32703 ; 1, 14 -- 12 Feb 2008 15:20:05 -0500 1, 14 -- Mime-Version: 1.0 1, 14 -- Message-Id: {p06240800c3d7a6c6b3b5-at-[141.212.131.221]} 1, 14 -- Date: Tue, 12 Feb 2008 15:20:02 -0500 1, 14 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 1, 14 -- From: Wil Bigelow {bigelow-at-umich.edu} 1, 14 -- Subject: [Microscopy] RE: Vacuum Grease 1, 14 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
Basically, the lifetime of ion getter pumps is determined by the amount of titanium available in their cathodes for producing the gettering action that is the basis of the pumps' operation, and also by the pressure range at which the pumps are operated. This matter is discussed in some detail in Sect. 7.1.8 (p 294) of my book Vacuum Methods in Electron Microscopy. As noted there, most manufacturers rate pump life on the basis of continuous operation at a pressure of 10^-6 Torr. Typical values are 45,000 to 50,000 hours (5 to 6 years). However, if the pump operates at a pressure of 10^-7 Torr (not unusual for pumps on the electron guns of modern instruments) then the operating life would be ten times as long, or of the order of 50 years. The development of "flakes", whiskers, and contamination on the insulators can shorten pump life, as mentioned by others. All of these factors are discussed in the above reference.. -- Wilbur C. Bigelow, Professor Emeritus Materials Sci. & Engr., Univ. of Michigan Ann Arbor, Michigan 48109-2136 e-mail: bigelow-at-umich.edu; Fx:734-763-4788; Ph:734-975-0858 Address mail to: 2911 Whittier Court Ann Arbor, MI 48104-6731
==============================Original Headers============================== 1, 14 -- From bigelow-at-umich.edu Tue Feb 12 14:40:04 2008 1, 14 -- Received: from skycaptain.mr.itd.umich.edu (smtp.mail.umich.edu [141.211.93.160]) 1, 14 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1CKe4eo028454 1, 14 -- for {microscopy-at-microscopy.com} ; Tue, 12 Feb 2008 14:40:04 -0600 1, 14 -- Received: FROM [141.212.131.221] (bigelow-g4.engin.umich.edu [141.212.131.221]) 1, 14 -- BY skycaptain.mr.itd.umich.edu ID 47B20422.33DAB.3474 ; 1, 14 -- 12 Feb 2008 15:40:02 -0500 1, 14 -- Mime-Version: 1.0 1, 14 -- Message-Id: {p06240801c3d7b048edf3-at-[141.212.131.221]} 1, 14 -- Date: Tue, 12 Feb 2008 15:40:01 -0500 1, 14 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 1, 14 -- From: Wil Bigelow {bigelow-at-umich.edu} 1, 14 -- Subject: [Microscopy] RE: Ion pump life 1, 14 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both wesley-smith-at-ukzn.ac.za as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: wesley-smith-at-ukzn.ac.za Name: James Wesley-Smith
Organization: University of KwaZulu-Natal, Durban, South Africa
Question: Dear colleagues If anyone out there has a Polaron E6300 vacuum evaporator, I would really appreciate if you could send me a scanned copy of the circuit diagramn needed for some troubleshooting.
Many thanks in advance!
James
Dr James Wesley-Smith Electron Microscope Unit University of KZN, Westville Campus Private Bag X54001 Durban 4000 South Africa
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Email: z.zhou-at-sheffield.ac.uk Name: Zoe
Title-Subject: [Filtered] carbon contamination while doing EELS
Question: I found bad carbon contamination while put Cr3C2 samples in a 2010 FEGTEM for EELS analysis. They were thin film cross sections made by conventional sandwich method by Epoxy glue, and Gatan PIPS ion milling. I have examined three samples made by the same route, two turned out contaminated easily, but one seemed not much affected. The samples were subjected to selected area EELS in diffraction mode and STEM ADF imaiging. Carbon accumulated at the edge of the selected area circle. Once the beam was focused, carbon K-edge was enchanced in the EELS acquired. Amorphous carbon in EEL spectra stops me from studying the real carbon bonding from the Cr3C2 phase.
My questions are: (1)Why were some samples contaminated, other not much? (2)What could be the most possible contamination sources? (3)How can I minimise the contamination both during sample preperation and TEM examination? (3)How reliable is the carbon data acquired?
I suspect that may be breaking down your carbide to form your amorphous carbon when you are focusing the beam onto the sample.
I had a sample of a pulsed laser deposited diamond-like carbon film. You can see an image of this film on our website in the application note #59 (http://www.southbaytech.com/appnotes/59%20EELS%20of%20PLD%20DLC.pdf) that was prepared by MicroCleaving(TM). The film was amorphous, but there are alternating light and dark bands in the DLC film. EELS showed that the darker bands were SP3 bonding while the light bands were SP2/SP3 or more graphitic. We only had a parallel EELS on a CM200FEG. When the spot was focused down in order to isolate a dark band, the earliest EELS spectra showed no Pi-star peak, but almost instantly, started growing one and a spot formed. If the beam was spread out, no contamination was seen. It was only in the highly focused beam that we got it and in the first instant, you could clearly see that the dark bands had no SP2 bonding. I always wanted to revisit these samples with a GIF to examine the sample without the conversion, similar to what Jim Bentley did with his GIF and carbon films.
Another question for you if you think that it is actual contamination is have you plasma cleaned your sample? If you always plasma clean your samples before putting them in the microscope and other samples do not contaminate in your microscope, then the source is not your sample and not your microscope, but is probably the mechanism that I described above.
For a source of contamination, check the O-rings and lubrication on your whisper lock on the PIPS. The same Ar gas that is used for the guns is used for the lift assembly. A colleague of mine has demonstrated conclusively that the Ar gas was contaminated by the lubrication from the lift mechanism in the PIPS. We strongly advise our customers who have either a Gentle Mill(TM) or an IV3/4 equipped with a low energy gun to not share the gas line with a PIPS instrument for that reason. Your difference in contamination could be the amount of time the samples were left in the PIPS. However, if you plasma clean your samples, you will eliminate this source or any other external source of contamination on your samples.
Disclaimer: South Bay Technology manufactures and sells the MicroCleave(TM) Kit and the PC-2000 Plasma Cleaner. We also distribute the Technoorg-Linda Gentle Mill(TM) and IV3/4 ion mills in the United States.
-Scott
Scott D. Walck, Ph.D. Technical Director South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673
US Toll Free: 1-800-728-2233 Tel: (949) 492-2600 Fax: (949) 492-1499
www.southbaytech.com walck-at-southbaytech.com
-----Original Message----- X-from: z.zhou-at-sheffield.ac.uk [mailto:z.zhou-at-sheffield.ac.uk] Sent: Tuesday, February 12, 2008 3:55 PM To: Walck-at-SouthBayTech.com
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both z.zhou-at-sheffield.ac.uk as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: z.zhou-at-sheffield.ac.uk Name: Zoe
Title-Subject: [Filtered] carbon contamination while doing EELS
Question: I found bad carbon contamination while put Cr3C2 samples in a 2010 FEGTEM for EELS analysis. They were thin film cross sections made by conventional sandwich method by Epoxy glue, and Gatan PIPS ion milling. I have examined three samples made by the same route, two turned out contaminated easily, but one seemed not much affected. The samples were subjected to selected area EELS in diffraction mode and STEM ADF imaiging. Carbon accumulated at the edge of the selected area circle. Once the beam was focused, carbon K-edge was enchanced in the EELS acquired. Amorphous carbon in EEL spectra stops me from studying the real carbon bonding from the Cr3C2 phase.
My questions are: (1)Why were some samples contaminated, other not much? (2)What could be the most possible contamination sources? (3)How can I minimise the contamination both during sample preperation and TEM examination? (3)How reliable is the carbon data acquired?
I have had the same experience with many different samples. The contamination is one the sample and why or how some are cleaner than others seems to be a mystery. However it must be correlated to cleanliness of glues, acetone, and alcohols used during preparation. Try to keep them clean, generally not sharing with others helps. After a soak, follow with a rinse of fresh liquid before drying with clean air.
If possible plasma clean your specimens before putting them in the scope. Any plasma cleaning will help, but I have found the H2/O2 recipes superior to Ar or Ar/O2.
I am assuming that you are using the liquid N2 cold trap on the 2010. Have is cold before inserting the sample.
If you have some old liquid freon in the lab, you can use it to rinse the sample before inserting in the TEM. This is a great help, but not for the environment.
If you do not have freon, or access to a plasma cleaner, and must work with the samples as they are, then you could try "locking the carbon in place on the surface of the sample". This might be done by using a large spot size beam spread over a relatively large area, to bake the carbon in place. I am not sure how well this works or for how long you have to let the beam set to be useful, but others will probably offer advice on this approach.
Good luck. You are not alone in your plight.
Roseann
Roseann Csencsits, PhD Donner TEM Facility Manager Lawrence Berkeley Lab 01-365 1 Cyclotron Road Berkeley, CA 94720 510-486-4548
On Feb 12, 2008, at 4:01 PM, z.zhou-at-sheffield.ac.uk wrote:
} } } Email: z.zhou-at-sheffield.ac.uk } Name: Zoe } } Title-Subject: [Filtered] carbon contamination while doing EELS } } Question: I found bad carbon contamination while put Cr3C2 samples in } a 2010 FEGTEM for EELS analysis. They were thin film cross sections } made by conventional sandwich method by Epoxy glue, and Gatan PIPS } ion milling. I have examined three samples made by the same route, } two turned out contaminated easily, but one seemed not much affected. } The samples were subjected to selected area EELS in diffraction mode } and STEM ADF imaiging. Carbon accumulated at the edge of the selected } area circle. Once the beam was focused, carbon K-edge was enchanced } in the EELS acquired. Amorphous carbon in EEL spectra stops me from } studying the real carbon bonding from the Cr3C2 phase. } } My questions are: } (1)Why were some samples contaminated, other not much? } (2)What could be the most possible contamination sources? } (3)How can I minimise the contamination both during sample } preperation and TEM examination? } (3)How reliable is the carbon data acquired? } } Zoe
==============================Original Headers============================== 17, 26 -- From RCsencsits-at-lbl.gov Tue Feb 12 23:25:04 2008 17, 26 -- Received: from ironport1.lbl.gov (ironport1.lbl.gov [128.3.41.47]) 17, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1D5P2Dk014947 17, 26 -- for {Microscopy-at-microscopy.com} ; Tue, 12 Feb 2008 23:25:03 -0600 17, 26 -- X-Ironport-SBRS: 4.7 17, 26 -- X-IronPort-Anti-Spam-Filtered: true 17, 26 -- X-IronPort-Anti-Spam-Result: AgAAAEIOskeAAykYk2dsb2JhbABBj3kBAQEBBwQGCSCcFw 17, 26 -- X-IronPort-AV: E=Sophos;i="4.25,344,1199692800"; 17, 26 -- d="scan'208";a="90460314" 17, 26 -- Received: from mta1.lbl.gov ([128.3.41.24]) 17, 26 -- by ironport1.lbl.gov with ESMTP; 12 Feb 2008 21:24:40 -0800 17, 26 -- Received: from [10.0.1.6] (c-76-126-228-210.hsd1.ca.comcast.net [76.126.228.210]) 17, 26 -- by mta1.lbl.gov (8.13.8/8.13.8) with ESMTP id m1D5Oc5U016080; 17, 26 -- Tue, 12 Feb 2008 21:24:39 -0800 (PST) 17, 26 -- Cc: Microscopy-at-microscopy.com 17, 26 -- Message-Id: {DC3023B7-51DF-4CE1-96D3-00D4CED6C527-at-lbl.gov} 17, 26 -- From: Roseann Csencsits {RCsencsits-at-lbl.gov} 17, 26 -- To: z.zhou-at-sheffield.ac.uk 17, 26 -- In-Reply-To: {200802130001.m1D01VlZ020494-at-ns.microscopy.com} 17, 26 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 17, 26 -- Content-Transfer-Encoding: 7bit 17, 26 -- Mime-Version: 1.0 (Apple Message framework v919.2) 17, 26 -- Subject: Re: [Microscopy] viaWWW: carbon contamination while doing EELS 17, 26 -- Date: Tue, 12 Feb 2008 17:34:42 -0800 17, 26 -- References: {200802130001.m1D01VlZ020494-at-ns.microscopy.com} 17, 26 -- X-Mailer: Apple Mail (2.919.2) ==============================End of - Headers==============================
The reason many microscopists don't want to use perfluorinated compounds in their electron microscopes goes back to the 1970s when diffusion pump oils based on these compounds were first introduced. (See Sect.5..4.5 p. 186 of "Vacuum Methods in Electron Microscopy") When first put into use as diffusion pump fluids it was found that these compounds broke down into small molecular fragments which were easily pumped out of the vacuum system, and were therefore essentially non-contaminating. Eureka! It was thought that the problem of oil contamination from diffusion pumps was solved. HOWEVER, after some use it was found that instruments using these fluids developed high-voltage instabilities due to micro-discharges along the ceramic insulators in their electron guns. This problem was more severe in TEMS, which operate at higher accelerating voltages, than in SEMs. About that time the Santovac polyphenyl-ether fluids came along, and most microscopists adopted them for use in their diffusion pumps..
I have not heard of anyone having this problem from the use of the Brayco or Krytox vacuum greases on O-rings, even on those in specimen stage mechanisms, however, and they are superb vacuum greases. They are excellent lubricants, they don't migrate or bleed, they are stable at temperatures up to around 250 C, and have vapor pressures in the 10^-10 Torr range.
I have not had any experience with the Santovac-5GB grease, but it sounds very interesting, too. -- Wilbur C. Bigelow, Professor Emeritus Materials Sci. & Engr., Univ. of Michigan Ann Arbor, Michigan 48109-2136 e-mail: bigelow-at-umich.edu; Fx:734-763-4788; Ph:734-975-0858 Address mail to: 2911 Whittier Court Ann Arbor, MI 48104-6731
==============================Original Headers============================== 3, 14 -- From bigelow-at-umich.edu Wed Feb 13 14:16:11 2008 3, 14 -- Received: from tombraider.mr.itd.umich.edu (smtp.mail.umich.edu [141.211.93.161]) 3, 14 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id m1DKGB0M005139 3, 14 -- for {microscopy-at-microscopy.com} ; Wed, 13 Feb 2008 14:16:11 -0600 3, 14 -- Received: FROM [141.212.131.221] (bigelow-g4.engin.umich.edu [141.212.131.221]) 3, 14 -- BY tombraider.mr.itd.umich.edu ID 47B35009.7B868.11765 ; 3, 14 -- 13 Feb 2008 15:16:09 -0500 3, 14 -- Mime-Version: 1.0 3, 14 -- Message-Id: {p06240802c3d8fa326965-at-[141.212.131.221]} 3, 14 -- Date: Wed, 13 Feb 2008 15:16:08 -0500 3, 14 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 3, 14 -- From: Wil Bigelow {bigelow-at-umich.edu} 3, 14 -- Subject: [Microscopy] RE: perfluorinated cmpds in EMs 3, 14 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
Position available at Binghamton University, one of the four university centers of the State University of New York, for an experienced electron microscopist to manage a facility that serves users in biological and materials sciences. Responsibilities include instrument maintenance (Hitachi H-7000 TEM, Hitachi S-570LB SEM), training users, service work. Applicants should have broad expertise in techniques for specimen preparation. Experience with light microscopic digital imaging also desirable. Bachelor's required; Master's preferred. Salary commensurate with education and experience. More information and application online at http:// binghamton.interviewexchange.com/. Screening of applications will begin March 1 and will continue until position is filled. The State University of New York and Binghamton University are Equal Opportunity/Affirmative Action Employers.
Dr. Curt Pueschel Associate Professor Department of